LOCUS       DACDPS010000001       136494 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.001, whole genome shotgun sequence.
ACCESSION   DACDPS010000001 DACDPS010000000
VERSION     DACDPS010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 136494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 136494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..136494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.001"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(136..2322)
                     /locus_tag="IWJ07_000001"
     CDS             complement(136..2322)
                     /locus_tag="IWJ07_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP9331612.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            2540..3223
                     /locus_tag="IWJ07_000002"
     CDS             2540..3223
                     /locus_tag="IWJ07_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAP9331613.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            complement(3304..4023)
                     /locus_tag="IWJ07_000003"
     CDS             complement(3304..4023)
                     /locus_tag="IWJ07_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP9331614.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(4335..4541)
                     /locus_tag="IWJ07_000004"
     CDS             complement(4335..4541)
                     /locus_tag="IWJ07_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9331615.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            4786..5388
                     /locus_tag="IWJ07_000005"
     CDS             4786..5388
                     /locus_tag="IWJ07_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAP9331616.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            5529..6809
                     /locus_tag="IWJ07_000006"
     CDS             5529..6809
                     /locus_tag="IWJ07_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP9331617.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            6822..7550
                     /locus_tag="IWJ07_000007"
     CDS             6822..7550
                     /locus_tag="IWJ07_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9331618.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(7619..9142)
                     /locus_tag="IWJ07_000008"
     CDS             complement(7619..9142)
                     /locus_tag="IWJ07_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP9331619.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            9394..10575
                     /locus_tag="IWJ07_000009"
     CDS             9394..10575
                     /locus_tag="IWJ07_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9331620.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(10635..12263)
                     /gene="treC"
                     /locus_tag="IWJ07_000010"
     CDS             complement(10635..12263)
                     /gene="treC"
                     /locus_tag="IWJ07_000010"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAP9331621.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(12714..15425)
                     /locus_tag="IWJ07_000011"
     CDS             complement(12714..15425)
                     /locus_tag="IWJ07_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP9331622.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(15435..16136)
                     /locus_tag="IWJ07_000012"
     CDS             complement(15435..16136)
                     /locus_tag="IWJ07_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9331623.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            16322..16921
                     /locus_tag="IWJ07_000013"
     CDS             16322..16921
                     /locus_tag="IWJ07_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9331624.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            17002..18015
                     /locus_tag="IWJ07_000014"
     CDS             17002..18015
                     /locus_tag="IWJ07_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9331625.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            18123..19040
                     /gene="rbsK"
                     /locus_tag="IWJ07_000015"
     CDS             18123..19040
                     /gene="rbsK"
                     /locus_tag="IWJ07_000015"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAP9331626.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            19068..19463
                     /gene="rbsD"
                     /locus_tag="IWJ07_000016"
     CDS             19068..19463
                     /gene="rbsD"
                     /locus_tag="IWJ07_000016"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAP9331627.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            19463..20836
                     /locus_tag="IWJ07_000017"
     CDS             19463..20836
                     /locus_tag="IWJ07_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9331628.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            21193..23337
                     /locus_tag="IWJ07_000018"
     CDS             21193..23337
                     /locus_tag="IWJ07_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9331629.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(23389..24429)
                     /locus_tag="IWJ07_000019"
     CDS             complement(23389..24429)
                     /locus_tag="IWJ07_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP9331630.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(24706..26508)
                     /locus_tag="IWJ07_000020"
     CDS             complement(24706..26508)
                     /locus_tag="IWJ07_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9331631.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(26522..27274)
                     /locus_tag="IWJ07_000021"
     CDS             complement(26522..27274)
                     /locus_tag="IWJ07_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9331632.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(27283..27645)
                     /locus_tag="IWJ07_000022"
     CDS             complement(27283..27645)
                     /locus_tag="IWJ07_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9331633.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(27821..28693)
                     /locus_tag="IWJ07_000023"
     CDS             complement(27821..28693)
                     /locus_tag="IWJ07_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAP9331634.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(28744..29232)
                     /locus_tag="IWJ07_000024"
     CDS             complement(28744..29232)
                     /locus_tag="IWJ07_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP9331635.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(29370..30200)
                     /locus_tag="IWJ07_000025"
     CDS             complement(29370..30200)
                     /locus_tag="IWJ07_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAP9331636.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            complement(30216..30824)
                     /locus_tag="IWJ07_000026"
     CDS             complement(30216..30824)
                     /locus_tag="IWJ07_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAP9331637.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(30817..31134)
                     /locus_tag="IWJ07_000027"
     CDS             complement(30817..31134)
                     /locus_tag="IWJ07_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9331638.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(31290..32018)
                     /locus_tag="IWJ07_000028"
     CDS             complement(31290..32018)
                     /locus_tag="IWJ07_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAP9331639.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            32177..33058
                     /locus_tag="IWJ07_000029"
     CDS             32177..33058
                     /locus_tag="IWJ07_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9331640.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(33295..34338)
                     /locus_tag="IWJ07_000030"
     CDS             complement(33295..34338)
                     /locus_tag="IWJ07_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9331641.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(34341..35009)
                     /locus_tag="IWJ07_000031"
     CDS             complement(34341..35009)
                     /locus_tag="IWJ07_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9331642.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            35151..37487
                     /locus_tag="IWJ07_000032"
     CDS             35151..37487
                     /locus_tag="IWJ07_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9331643.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(37602..37913)
                     /locus_tag="IWJ07_000033"
     CDS             complement(37602..37913)
                     /locus_tag="IWJ07_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9331644.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            38094..39569
                     /locus_tag="IWJ07_000034"
     CDS             38094..39569
                     /locus_tag="IWJ07_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAP9331645.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            39616..40200
                     /locus_tag="IWJ07_000035"
     CDS             39616..40200
                     /locus_tag="IWJ07_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAP9331646.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            40202..40621
                     /locus_tag="IWJ07_000036"
     CDS             40202..40621
                     /locus_tag="IWJ07_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAP9331647.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(40902..41186)
                     /locus_tag="IWJ07_000037"
     CDS             complement(40902..41186)
                     /locus_tag="IWJ07_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAP9331648.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(41538..42248)
                     /locus_tag="IWJ07_000038"
     CDS             complement(41538..42248)
                     /locus_tag="IWJ07_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAP9331649.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(42245..43717)
                     /locus_tag="IWJ07_000039"
     CDS             complement(42245..43717)
                     /locus_tag="IWJ07_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9331650.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            43855..44328
                     /locus_tag="IWJ07_000040"
     CDS             43855..44328
                     /locus_tag="IWJ07_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9331651.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(44481..45032)
                     /locus_tag="IWJ07_000041"
     CDS             complement(44481..45032)
                     /locus_tag="IWJ07_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9331652.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            45137..45667
                     /locus_tag="IWJ07_000042"
     CDS             45137..45667
                     /locus_tag="IWJ07_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAP9331653.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(46150..46593)
                     /locus_tag="IWJ07_000043"
     CDS             complement(46150..46593)
                     /locus_tag="IWJ07_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9331654.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(46609..47769)
                     /locus_tag="IWJ07_000044"
     CDS             complement(46609..47769)
                     /locus_tag="IWJ07_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9331655.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            48276..49976
                     /locus_tag="IWJ07_000045"
     CDS             48276..49976
                     /locus_tag="IWJ07_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9331656.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(50078..50911)
                     /locus_tag="IWJ07_000046"
     CDS             complement(50078..50911)
                     /locus_tag="IWJ07_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAP9331657.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(51215..53380)
                     /locus_tag="IWJ07_000047"
     CDS             complement(51215..53380)
                     /locus_tag="IWJ07_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9331658.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            53640..55934
                     /locus_tag="IWJ07_000048"
     CDS             53640..55934
                     /locus_tag="IWJ07_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP9331659.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            55927..56592
                     /gene="pgmB"
                     /locus_tag="IWJ07_000049"
     CDS             55927..56592
                     /gene="pgmB"
                     /locus_tag="IWJ07_000049"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP9331660.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            56585..57592
                     /locus_tag="IWJ07_000050"
     CDS             56585..57592
                     /locus_tag="IWJ07_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP9331661.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            57614..58633
                     /locus_tag="IWJ07_000051"
     CDS             57614..58633
                     /locus_tag="IWJ07_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9331662.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(58914..60281)
                     /gene="nhaC"
                     /locus_tag="IWJ07_000052"
     CDS             complement(58914..60281)
                     /gene="nhaC"
                     /locus_tag="IWJ07_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP9331663.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(60390..61811)
                     /locus_tag="IWJ07_000053"
     CDS             complement(60390..61811)
                     /locus_tag="IWJ07_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9331664.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(62054..63931)
                     /gene="tdc"
                     /locus_tag="IWJ07_000054"
     CDS             complement(62054..63931)
                     /gene="tdc"
                     /locus_tag="IWJ07_000054"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP9331665.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(64222..65478)
                     /locus_tag="IWJ07_000055"
     CDS             complement(64222..65478)
                     /locus_tag="IWJ07_000055"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAP9331666.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            66058..67359
                     /locus_tag="IWJ07_000056"
     CDS             66058..67359
                     /locus_tag="IWJ07_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP9331667.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(67428..68027)
                     /locus_tag="IWJ07_000057"
     CDS             complement(67428..68027)
                     /locus_tag="IWJ07_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9331668.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            68281..69162
                     /locus_tag="IWJ07_000058"
     CDS             68281..69162
                     /locus_tag="IWJ07_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9331669.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            69175..70785
                     /locus_tag="IWJ07_000059"
     CDS             69175..70785
                     /locus_tag="IWJ07_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAP9331670.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(70862..71521)
                     /locus_tag="IWJ07_000060"
     CDS             complement(70862..71521)
                     /locus_tag="IWJ07_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP9331671.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(71804..72469)
                     /locus_tag="IWJ07_000061"
     CDS             complement(71804..72469)
                     /locus_tag="IWJ07_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9331672.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(72616..73221)
                     /gene="dhaL"
                     /locus_tag="IWJ07_000062"
     CDS             complement(72616..73221)
                     /gene="dhaL"
                     /locus_tag="IWJ07_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAP9331673.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(73249..74232)
                     /gene="dhaK"
                     /locus_tag="IWJ07_000063"
     CDS             complement(73249..74232)
                     /gene="dhaK"
                     /locus_tag="IWJ07_000063"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAP9331674.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(74234..74629)
                     /gene="dhaM"
                     /locus_tag="IWJ07_000064"
     CDS             complement(74234..74629)
                     /gene="dhaM"
                     /locus_tag="IWJ07_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAP9331675.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            74783..75103
                     /locus_tag="IWJ07_000065"
     CDS             74783..75103
                     /locus_tag="IWJ07_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAP9331676.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(75215..76084)
                     /locus_tag="IWJ07_000066"
     CDS             complement(75215..76084)
                     /locus_tag="IWJ07_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9331677.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(76180..77484)
                     /locus_tag="IWJ07_000067"
     CDS             complement(76180..77484)
                     /locus_tag="IWJ07_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9331678.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(77481..77990)
                     /locus_tag="IWJ07_000068"
     CDS             complement(77481..77990)
                     /locus_tag="IWJ07_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9331679.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(78165..80300)
                     /locus_tag="IWJ07_000069"
     CDS             complement(78165..80300)
                     /locus_tag="IWJ07_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9331680.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(80383..82038)
                     /locus_tag="IWJ07_000070"
     CDS             complement(80383..82038)
                     /locus_tag="IWJ07_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9331681.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(82040..82354)
                     /locus_tag="IWJ07_000071"
     CDS             complement(82040..82354)
                     /locus_tag="IWJ07_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP9331682.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            82524..82784
                     /locus_tag="IWJ07_000072"
     CDS             82524..82784
                     /locus_tag="IWJ07_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAP9331683.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            82798..83112
                     /locus_tag="IWJ07_000073"
     CDS             82798..83112
                     /locus_tag="IWJ07_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAP9331684.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(83842..84396)
                     /locus_tag="IWJ07_000074"
     CDS             complement(83842..84396)
                     /locus_tag="IWJ07_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAP9331685.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(84522..86450)
                     /locus_tag="IWJ07_000075"
     CDS             complement(84522..86450)
                     /locus_tag="IWJ07_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP9331686.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(86756..87472)
                     /locus_tag="IWJ07_000076"
     CDS             complement(86756..87472)
                     /locus_tag="IWJ07_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP9331687.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(87473..89296)
                     /gene="glpO"
                     /locus_tag="IWJ07_000077"
     CDS             complement(87473..89296)
                     /gene="glpO"
                     /locus_tag="IWJ07_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAP9331688.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(89310..90806)
                     /gene="glpK"
                     /locus_tag="IWJ07_000078"
     CDS             complement(89310..90806)
                     /gene="glpK"
                     /locus_tag="IWJ07_000078"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAP9331689.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(90942..92402)
                     /locus_tag="IWJ07_000079"
     CDS             complement(90942..92402)
                     /locus_tag="IWJ07_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9331690.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(92392..93687)
                     /locus_tag="IWJ07_000080"
     CDS             complement(92392..93687)
                     /locus_tag="IWJ07_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9331691.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(94019..94696)
                     /locus_tag="IWJ07_000081"
     CDS             complement(94019..94696)
                     /locus_tag="IWJ07_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAP9331692.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(94726..95817)
                     /locus_tag="IWJ07_000082"
     CDS             complement(94726..95817)
                     /locus_tag="IWJ07_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAP9331693.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(95918..96352)
                     /locus_tag="IWJ07_000083"
     CDS             complement(95918..96352)
                     /locus_tag="IWJ07_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9331694.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(96936..98393)
                     /gene="ascB"
                     /locus_tag="IWJ07_000084"
     CDS             complement(96936..98393)
                     /gene="ascB"
                     /locus_tag="IWJ07_000084"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP9331695.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(98390..100285)
                     /locus_tag="IWJ07_000085"
     CDS             complement(98390..100285)
                     /locus_tag="IWJ07_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9331696.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(100343..102118)
                     /locus_tag="IWJ07_000086"
     CDS             complement(100343..102118)
                     /locus_tag="IWJ07_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9331697.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(102168..103664)
                     /locus_tag="IWJ07_000087"
     CDS             complement(102168..103664)
                     /locus_tag="IWJ07_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9331698.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(103858..105480)
                     /locus_tag="IWJ07_000088"
     CDS             complement(103858..105480)
                     /locus_tag="IWJ07_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP9331699.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(105929..107242)
                     /locus_tag="IWJ07_000089"
     CDS             complement(105929..107242)
                     /locus_tag="IWJ07_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP9331700.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(107487..107711)
                     /locus_tag="IWJ07_000090"
     CDS             complement(107487..107711)
                     /locus_tag="IWJ07_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9331701.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(107731..108099)
                     /locus_tag="IWJ07_000091"
     CDS             complement(107731..108099)
                     /locus_tag="IWJ07_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP9331702.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(108296..109006)
                     /locus_tag="IWJ07_000092"
     CDS             complement(108296..109006)
                     /locus_tag="IWJ07_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9331703.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(109024..109521)
                     /locus_tag="IWJ07_000093"
     CDS             complement(109024..109521)
                     /locus_tag="IWJ07_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9331704.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(109558..110010)
                     /locus_tag="IWJ07_000094"
     CDS             complement(109558..110010)
                     /locus_tag="IWJ07_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9331705.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(110145..110750)
                     /locus_tag="IWJ07_000095"
     CDS             complement(110145..110750)
                     /locus_tag="IWJ07_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP9331706.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(111200..111820)
                     /locus_tag="IWJ07_000096"
     CDS             complement(111200..111820)
                     /locus_tag="IWJ07_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9331707.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(111822..112691)
                     /locus_tag="IWJ07_000097"
     CDS             complement(111822..112691)
                     /locus_tag="IWJ07_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAP9331708.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(112714..113121)
                     /locus_tag="IWJ07_000098"
     CDS             complement(112714..113121)
                     /locus_tag="IWJ07_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP9331709.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(113140..114429)
                     /locus_tag="IWJ07_000099"
     CDS             complement(113140..114429)
                     /locus_tag="IWJ07_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9331710.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(114456..114758)
                     /locus_tag="IWJ07_000100"
     CDS             complement(114456..114758)
                     /locus_tag="IWJ07_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9331711.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(114783..116876)
                     /locus_tag="IWJ07_000101"
     CDS             complement(114783..116876)
                     /locus_tag="IWJ07_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9331712.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(117365..118129)
                     /locus_tag="IWJ07_000102"
     CDS             complement(117365..118129)
                     /locus_tag="IWJ07_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAP9331713.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(118240..119109)
                     /gene="mdcB"
                     /locus_tag="IWJ07_000103"
     CDS             complement(118240..119109)
                     /gene="mdcB"
                     /locus_tag="IWJ07_000103"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAP9331714.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(119134..120210)
                     /locus_tag="IWJ07_000104"
     CDS             complement(119134..120210)
                     /locus_tag="IWJ07_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAP9331715.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(120234..120827)
                     /locus_tag="IWJ07_000105"
     CDS             complement(120234..120827)
                     /locus_tag="IWJ07_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAP9331716.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(120831..122507)
                     /gene="mdcD"
                     /locus_tag="IWJ07_000106"
     CDS             complement(120831..122507)
                     /gene="mdcD"
                     /locus_tag="IWJ07_000106"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAP9331717.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(122497..122796)
                     /locus_tag="IWJ07_000107"
     CDS             complement(122497..122796)
                     /locus_tag="IWJ07_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAP9331718.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(122898..124544)
                     /gene="mdcA"
                     /locus_tag="IWJ07_000108"
     CDS             complement(122898..124544)
                     /gene="mdcA"
                     /locus_tag="IWJ07_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAP9331719.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(124551..125462)
                     /locus_tag="IWJ07_000109"
     CDS             complement(124551..125462)
                     /locus_tag="IWJ07_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP9331720.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            125582..126439
                     /locus_tag="IWJ07_000110"
     CDS             125582..126439
                     /locus_tag="IWJ07_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9331721.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(126626..126988)
                     /locus_tag="IWJ07_000111"
     CDS             complement(126626..126988)
                     /locus_tag="IWJ07_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9331722.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(127177..127359)
                     /locus_tag="IWJ07_000112"
     CDS             complement(127177..127359)
                     /locus_tag="IWJ07_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9331723.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            127334..128773
                     /locus_tag="IWJ07_000113"
     CDS             127334..128773
                     /locus_tag="IWJ07_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9331724.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(128780..131332)
                     /locus_tag="IWJ07_000114"
     CDS             complement(128780..131332)
                     /locus_tag="IWJ07_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAP9331725.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPKGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYELGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(131446..132777)
                     /locus_tag="IWJ07_000115"
     CDS             complement(131446..132777)
                     /locus_tag="IWJ07_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9331726.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(132743..134221)
                     /locus_tag="IWJ07_000116"
     CDS             complement(132743..134221)
                     /locus_tag="IWJ07_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9331727.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(134526..136361)
                     /gene="tdc"
                     /locus_tag="IWJ07_000117"
     CDS             complement(134526..136361)
                     /gene="tdc"
                     /locus_tag="IWJ07_000117"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP9331728.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
BASE COUNT        39873 a        28384 c        23827 g        44410 t
ORIGIN      
        1 ttttccgaat aaactgtgtt ccagaaatag cttcttcgaa aataagctga aatttcacaa
       61 aaatttgaga agcaattttc gtacattcct tcttctttct tgaaactgac cacttctgtc
      121 acaccctctc ttctatcact ttcctttttt cacacgactc ccggtttctt tgataaatct
      181 cgatactttc aattttcttg aatttttcat ttgaggtaca catagataca gacgatttct
      241 tgctctagtc atcgcaacat aaaatagtct tcgttcttct tctatcgtcg tcactggttc
      301 tgcttttatt tcattaaagt cgattacttt cttttctgtc gctccgttat aaggcaaact
      361 tccttcgtta caaccaatca aaaagacatt tcggtaacta agacctttac ttttgtgaat
      421 gctctggata catagtgcat caagttttcc ttgtctgtaa gcaatcaagc gggtttttaa
      481 ttctttagct gtcgcttggg tttcttgcga taaggcttgg agtcttctga aagtagcatg
      541 ctgctttact tcttctcgta tccattcttg atcactctgg tcgatttcct tagctatacg
      601 tggcagatga agatagcttt gaacaagcga atagatttct tgatgctggt ctgttttacg
      661 caacgcttcc tcaaactctc ggatttctgc agatttcgat tttaaattgg ggcttatcga
      721 caaaatataa tctgcaacgg ctatattttg atatttagga gaaaagtatt gatcgcattc
      781 actttttttc aggtagcagt ctgccatcag ctgccggagc aattgctggt aggcctgatt
      841 tttcttttgc agattcagtg ctgtttgcca atgaacaatt ttttgataga aatgttggaa
      901 agacttgata ccaaagatac cttcatatgg actatctgct tccagtacaa aaggaatatt
      961 cgttttcagc agagattgga caagctgctg cgtttgtgaa gctgatcggt ataatatgat
     1021 tgattcctta tatttcccat ttttttgaat aaatgtctga atttgtttga ttagccactg
     1081 actttctgct ttagtatcag aaaacaaata ggcttccata acacctgctt ctttttgtgc
     1141 tggaatcatc gcctttggta tccgatcatt tttattatag ctgatcaatc tatttgctcc
     1201 ttggacaata gctgcccgag aacgataatt agttaataag aaaagagttt tggtaggata
     1261 ttctttttca aagttcttga tgtatgctgg ctctgatcct ctgaatccat agatactctg
     1321 atcatcatct ccaacaacaa tcaacttgat cctgttttcc tttgtcatcc gtttcacgat
     1381 ttcccattga agtggattag tatcttgaaa ttcatcaatg aatagataat catatttttg
     1441 gccgatataa tgctggatct cctcattctc caaagcgctt ttcagatttt ggagtacatc
     1501 attgaactct agcaattgct ggcttctttt ttgttcttgg agttccgtga gcaatccata
     1561 cagtcgttct gatatcggct gtcgtgcatt cttatcaatg gaatcatatt tttctttaac
     1621 taactgaacc atttcttcta ctgaaaggtt catattctgc caatagtcta accgagaagc
     1681 aatcgttttt tctccatatt gatcaaatat ataaagatta tatttacgta tcagctgttg
     1741 caaaacagca ttcacttttt gtgcagtatc ctttgaaaag tctccttgta ttccttgcat
     1801 tgctacttca ggaaattttc gacaaatttc attgtatatc ctaaaaaaga tactatggaa
     1861 tgttccgata tggacagcgg tttttctctg gctgaaatta tctactcgtt tttttagttc
     1921 atttgcagcg ttccttgtaa aagtgatcat tagtatgcgt tcttcgtcta cttgtttttg
     1981 cgtcatcaga taaacagctt ttgcacagat tgtttgcgtt ttgccgctcc cagctcctgc
     2041 tatgattcga agatgtttct tcatatcata tgtgatagct gctttttgtt cttgattgaa
     2101 gcgattatag gaacgtttct ccatcatttt aaagaaagag ctgtttcctt cactttcttt
     2161 tacttgtact tcttcctcaa taagtccggg tgcttcgcca taagccgctt cataggtagc
     2221 aatgatttct tcttgtagcc gaaagacttg ttgaagcttt ttatacttgt tctcccagcg
     2281 tttcaattct ttttgtaatc ggagaatttc tttttcattc aactgattga tttccttccc
     2341 atctttcttc tttgaatttg tttatttcaa gttggttatt taaagtttcg ttgtctattt
     2401 tttctttata ctaacagacc tttctaaaaa tgaaaatggg aagcgatatg attttagttt
     2461 tctgaaaatt tttccttttt ttcaatcgtt tatgctaaga taaggaagac aaaaatattc
     2521 ataaaaggtg atgaaaaaaa tgcagacttc ctttcacgag gggcgtacaa taggactttt
     2581 gctgacattt atcggaggag ccatggacag ttatacttat atccaatatg gtgcttttgc
     2641 ctctgctcaa actggcaata tcattctatc gatcatccaa gcatttgacg gagaatggct
     2701 aagtgttggt aaaaaagtat tgtctaccct tttctttttc ttagggattc ttctaacaaa
     2761 gtatctaatt gattatttta gaaaaaaaga aaaacatttc tggcgcttgt ttgttctata
     2821 ttatgaagca gtgatttttt tctttgttag tttgacaccc gtccatgagc atcccgcatt
     2881 agtaacgatt ttgattgctt ttactgcatc gatccagtgg gtagcttttg ataaaatcaa
     2941 cgggcgtgcc tacactaatt tatttacaac tggaaattta aaaggggtag caacgaactt
     3001 atatgattat tgtatttcta aagaaaaagc agattttgat cgatttttcc actatctaag
     3061 tgtcgttctt gcctttatcg caggagctat tgtccttgtt ttctgtcatc atctgtttgg
     3121 cgcccattcg attttgatcg tttccatttt atttttgatc atggccctca ctcagacatt
     3181 tcatttatgg caattttatc atacgaatcg gttcactatt tgatctcaat gaatcaaaaa
     3241 aactaagcaa gatgctgctc acagagaaat atctgcgagt gagcaatttg cttagttttt
     3301 atattattta gttgtaaaga ttccctttgc tttcgggtta tttgtagtgt agcggatcga
     3361 ctgggcatct gcctcttctg tcattttctt tgcccgcatc ttgatttctt gtacgatttg
     3421 ttctccacat gccggatcgg ctacgaaatc atatttgtcc ccatcgatca ctattttggg
     3481 acatatatca aactgttcat accactcttc gtatcgtcga ttgagttctt tatagtagga
     3541 atagagttca ggatcttgtt ctagctgttc atattcccgg cctctttttt tgattctcgc
     3601 taacatagtt tcaaaggata cacgaatata aaccagtaga tctggtttct tttgtggcga
     3661 aatttcttct aactccttca gcatcgtttc taaaagacct tcatactgct ttacttctac
     3721 ttcagaaaca cgccctaaat ctgcattcaa atgaaacagc atactatctt cgtagatgga
     3781 gcggtctagt acgttgtcat catggctgaa agcatctttg attgccaaaa agcgtttgtt
     3841 caaaaagaaa atctgtaata aaaaagtata cttttctgga ttagaataaa atagcggcaa
     3901 tacttcatta tcttccacgt tctcataaaa aggttttgtt tctaaatctt gggcaagcat
     3961 ttcagttagt gtgcttttcc ctgcaccgat cgttcctgca acaatgatca caattatcgg
     4021 catccttcct gtaataaaat ctgttcttca ttttactact tcatttatag aaataaaagc
     4081 cttgacttta tctgctcctt aaaagctact tctctaaaag tagccaaaag taactcctat
     4141 tcatgaaaat aaaggctgtg acaaaagtct tgtcgcagcc cttttttcaa ataaacagta
     4201 ttctcctgcc aacacctcgg tcacaaggca atattcataa aacatgaaaa gctgtttttt
     4261 aacattattc cttgtaactc agagctaaac gggagactca caacctcttt atgctctcaa
     4321 cattttatct gtttttattc ttctattgta cttgatttgc gtacaactaa ctgccagtgc
     4381 agattatgag cggtatcatt gtcttctact gcgcatttca tgacacgtat catcacacta
     4441 ttttcataga ttttttctat ttttcctttt attggtgatt caaataaatc agatttaaat
     4501 gcatatgttt cgccgacttc aaacaattgt tcattttcca taagaatccc tccatcacct
     4561 tttataaggt cagtaatttt ttaacataaa tcactcgaaa aagcacaaac ttgtcataaa
     4621 aagtactttt cgtatcaaaa ctgtcacttt ttttggtaag gttcttggaa aacgaagatt
     4681 tttttaggag atcatgacgg ttttgatgtc aaaaccactt tttatgaaac aaaagacgtt
     4741 ttttattttt tttagcataa aatttgtggt ggaggtacgt atgagatgaa agataaagtt
     4801 gtgaacaaat tattagaagc cttattcagc ggagacaaat cggaagaaga tatggtgtat
     4861 gtccaagtaa aatttgtctt agaagtttta ttgaataatc ttggaaaact agcggtcgtt
     4921 ctagtctttt ctctggtgac aggctcatgg gcggaaacag gaatcacctt tctttcttat
     4981 atctgcatcc gcagatatgc ttatggattg cattccgact ctgaattcgt ttgtctgctg
     5041 tggacactac tttatttatg gggcgttccc ttggtgatga aacatctgca attgactatc
     5101 tccttcccaa tgatggtctt tttgttaatc agctgctttt tgttactgct tcgctatgga
     5161 tcaagaggta cagcaatcaa tcccattgaa cccgagaaac gtcccccatt attaaagaag
     5221 gcgatcagca tgtttctaat tttttctctc attacgttat ttttctcagc ttcttacttt
     5281 tctacctatt tgctacttgg gattgtttta gaaatagcta cgttgcttcc tattacaaac
     5341 tacttgatga attttggagg aatctttcat gaaaaaaaca atcattaaac aattaactaa
     5401 cttatctgac ttttttgtat ctggctgctg ctttggtatg agttctgaat tcaaaatccc
     5461 tgaaaaatta aaaaaataat acgccaattg tgctataatt tggtaaaaat caatgggagc
     5521 tgtactttat gtttttatgg tccatagcgt tgcaatattt gatttacaca atttgtatct
     5581 tattgattga ccatcacatc cgatacaaat atctgtttgc gggagtcatt ctgatttctt
     5641 gtattttgat tggatgggtc tattcgattt tagggatttt tactgctcta attgctatag
     5701 gaatgctgat gcttactttt ttcttctttt cgacttcgcg tcaacaattg gtttacgcgc
     5761 ttccttttgg gttattgacg tcgcttttag gagatcactt aacaagcatc ggcgattttt
     5821 ttgttttgaa atcttcggtt attgaaccag gagattcatt gcagtatttt cacattgtct
     5881 tatccgcttt tttatctgct ttattagctt tttgtttaaa aaaatcgtta gagacttggt
     5941 ttcacactgt ggaccgccgt atgatcggta cgatcgggac attgatatta tttacatact
     6001 atatcattat tttttatacc cgtttttctg gtgaaacacc tgatgtctta gctttgaata
     6061 cgagtttttt cattctatat ctgtgtgttg gcctgatcat cttggtttat tattggaaaa
     6121 tctctaataa gaaattagct gatcagttat tagagcaaca agtcaggatg caacaggatt
     6181 atatcaaaga tctagaaaaa aactatcaag acttgagaga attcaaacat gattaccaaa
     6241 atctgctttt ttctcttcac agctatattt cagaaggcga cttagaagga ttacggtctt
     6301 attatgatca aacgatatta cctacacgcc agatgatgaa cctttttccg gccaatctaa
     6361 gtatgttgga taatatgaaa gttcctgaga tccgtagttt actgtcatta aaactcatga
     6421 tggcacagga aaaagggttg atcgtccagc ttcattttcc tgagccaatc gaactggata
     6481 cgaaacatac ggtaaacctt gtacgtatgc ttggtattat cttggacaat gcgattgaag
     6541 gagctagtgc tgcaaagaag aaaaaaatcg aattgaccat actcaagaag aatcattccg
     6601 tgatgattcg tgtgatcaat acaacaacaa atacattacc gttaaaccag ttgaaacaaa
     6661 aagggttctc aacaaaaaca aatccaaaaa acgaaggcct tggcttgttt atcttggatg
     6721 aattgacaaa gaccaatcct tatcttttcc ttgaaacatc aattgaaaac cagcgttttt
     6781 ctcaaacgat ttatatacaa ataaaataaa ggagtaatga tatgattccc atttatattt
     6841 gtgaagacga tccaaaacaa ttagaagttt tatcaacggt gatcaaaaat agaatcatga
     6901 ttgaaaactt agacagttat gtccatattt ctacctctga tccggaagaa cttctcaatg
     6961 cagccaagat cagaacttct tcttttggta tttacttttt agatatcgaa ctagaagaca
     7021 gtgaaatgga tggtgtagcg atcggtaaat tgatccgtga attagatcct ttaggaaaaa
     7081 taatctatgt gacttcccat acggaaatgt cgatgaaaat cttaaaaagc aacatcgaac
     7141 cgactgatta tatcgtcaaa gaagatcttt atgatttaaa agaaaatgtc gagcatatcc
     7201 tgagcaaaat tttcgaagat tttcaaatgt cccaaatcca aaaagatatc ttcaaaatag
     7261 agtttaatga tgagatcaaa tttttaccga tcaaagacat ccaatatttt tctacttcgc
     7321 taggagcgcc acataaactg gaagtccatt tgactcatgc ccaattacaa ttctatgaga
     7381 agatcaaaga aatcgagaaa atgcatcgga attttatccg ttgtcataaa tcctatgtta
     7441 tcaatacaca aaatgtgcgg tcgattaata aaaaaaccag agaagtcacg ttggcaaacg
     7501 gcgagaccat cccagcctct atccgtggat taaagaaatt gatttcataa aaagaaccga
     7561 atagtttgga ccgactaaat gaccagccga tccaaaccct tcggttcttt tttgcctatc
     7621 agtgcaaaag catttcctgt gcaatttcac gtccatccat tgtatctgga taaaatgttg
     7681 gccaattctc catttctttc agcaatactt cttgactttc atttcctgca tataaatgaa
     7741 aatgttcgga gtcagccggt gcaatcgtgt gatcactgaa ttgaatatat ctaaaagcgc
     7801 cactttcctt atcttctgct tcgaatagat accttacgcc acggtttcct gcctcgtagg
     7861 tcaagatttc tttccctaca taacgataat ttgaacgttt cgttgttcca ttcttaaacg
     7921 taaatgccat ctctttctca ctgatcttga ttgaagcaat atctgtttga tagcccttcg
     7981 tataatattc tttatattct tgtgctgtct tatcctgatt caatttttct ttatattcaa
     8041 aaacaggatc aaaagtccca tcttctacat acgtatctac tgtttgccaa gttcctgccc
     8101 aatctgaaag agagcgatct tgtacatcct cgtcttcaaa ataaccgttg tagacgctct
     8161 tttctttttt gggggtcaaa gtatcttcta gcgggttttc tgtctctgtc gtttttttca
     8221 acgcttcgag gtttgcttcc atgatagaaa gatagttttc tccattttct atttgttcat
     8281 tcgtcaatcc ttccaaagga ttcaatactt ctaaagatac acctgcttcg tttgctaacg
     8341 ttctggcaat actatctttg gcgttttctt caaaataaat atagttgatt ccatgttctt
     8401 taacaaagtg tttcagttca gcaattctcg ccgcagacgg ttcttcacta gaagaaagac
     8461 cagcaatcga tacttgattc aatccatatt ctaacgctag atagttgaat gcagcatgtt
     8521 gcgtgacaaa agatctttgt tttgcttgct taaaggtttc ttgataaagc tgatggagtg
     8581 cttgaagttt ctcggtatag gctgctgcat tttttgtcca tacttcctgt ttttctgggt
     8641 aagcttcaat cagctgatca cgtatgtttg ctacctgctt gattgctaaa cttggagcta
     8701 gccatacatg tgggtctagc tcatgtgtat ggccttcttc cccgtgttca tggtcatgat
     8761 catgtccttc ttcacttccc ggaagtaaaa gcataccttc tgtcgctttt atcgtatgga
     8821 cattgccttc ttgtaatgtt ttttggattg cggggaccca tgtttccatg ttttcatcat
     8881 tataaacaaa gatatctgta tcttggatct ttgccatgtc tttagcagaa ggttcataat
     8941 cgtgggaatc agtccctgct ggaatcaata actctacttc tccttcatcc ccgacgattg
     9001 cttttgtaaa atcatacatg ggataaaagg tcgtcatcac gctcaatttt tctttactgt
     9061 cctcggcttt cccacttgcc ccgcaagcag ttaacatacc tgctaaaacc agcatggcca
     9121 atatacctat caatcgtttc atttatgaca ctcctcttct catcttcttt catttgaatg
     9181 ttccgattcg cttttcgcaa accgtaatag ttacgtttta gcaacaggat ttaatttatc
     9241 atatggttaa aagaactgtc aagcgttttc ttattttaat tgatgagaaa gtttatatga
     9301 atgattgacg atatttttgc aatcaacctc tatcattttc cttttatacc cgcttttgtc
     9361 tataatgaag ttatagataa aggaggaaaa agagtgaaaa aagaaacaga tacaacgaat
     9421 cgtttgatcc gctttttagg aggaaaaacc tcttactatg ttttaggaat agttatctta
     9481 cttgcgctga tcatttattt tttccatcag atctcgttta tattcaagcc tgtcggaatc
     9541 attatcgcta ctatcttgcc cccattattg tttgcactga tcttgtatta tcttttggaa
     9601 ccagtcatca aacgtttgag caagcacctt tcgaggaact tgtcagtcat tatcgtttat
     9661 gtgattattt tatttttatt ggggcttgga agcatatggc ttattccttt tcttgaagaa
     9721 caggcaaaga cattgatcag tagtttgcca gatttgtttc aagattttca agagagtctc
     9781 cgacaatttt tggagaaaac accttttgcc gattcattta atcaattttt cgcttctatt
     9841 gacgacgtga cgtctgatat agctggattt attggaaatt attgggaaag tggcgcacaa
     9901 cggatcagca acattttttc aactgttaca gctattttta ttactttatt tactggtcca
     9961 attgtcgctt ttttcctttt gagagatcct caaaaatttt atcagtcagt tttagcgatt
    10021 gtcccaccag catttcgtac agattttaaa aatttgacaa agattgctaa tcaacaattg
    10081 ggttcatttc taaaggggca aatcattgct tcatttatcc ttggagcagt ctactgggtt
    10141 tgtttcttac tgatcggatt agaatttgct agtgttttag ctatttctgc tgggttgtta
    10201 tgtatcattc catatattgg gccgtttatc gccttctttc caggtttgat cattgcattc
    10261 caagactcta cttttatggc tgtcaaattt gtcattgtct ggtttgccgt acaacttctc
    10321 cacggtgact tggtgattcc tcgagtaatg ggtgatcgtt tacagatcca tccgattacg
    10381 atcttaatcg ttttacttgt catgggtgat ctaatgggaa ttgtgggcgt tatttttggt
    10441 attcctatct atacgttagt caaattactc gttactttta ttttccgtaa attcaaacaa
    10501 cgctacaata aattttatgg cgataaagga gagtatgaaa actcttcatt ttctgaagaa
    10561 gattatttta aataaaacac aaaacgtcga tcttacgaga atagctcgta ggaacgacgt
    10621 tttgtgtttt tgttttaatt tttcagcaaa gcaattgcct ggtatggttt tagctccaga
    10681 ctttgatgaa gaacagtatc ggaataatta tcgataagaa tattggcttc tgtgaatcct
    10741 tctgggatgg cgatccttgt atctttatca tagaaatttg ttaatacaag cagctgctgt
    10801 ccttcaaatt ggcgaagaaa gccataaact tccggatgat ttgcttcata agcatgataa
    10861 tctccttgtg caacgattgg atatgttttt ctcagccgaa tcaatttctt gtaataggac
    10921 aaaatagaat cttcagaaga tagttctttt tctacattaa tctcttctag cttgcctcca
    10981 acttttaacc aaggtttccc tgtagtaaat cctgcttgtt ctgtgtctga ccattgcatg
    11041 ggtgttcgag agttatcgcg agattttgct ttgatccgac gaaacgcttc tttttctgtg
    11101 aatccttgtt caaggagtaa cttataagca ttcaaacttt ctacgtcaat atagtcggaa
    11161 atcgatgaat agtctgggtc gatcatgccg atttcttctc ccatataaat atagggcgtc
    11221 ccacggttca aatggatcat ggttgctagc atctctgctc cttgttttcg atactgatcg
    11281 atagctacaa atcgattgag cgcacgtggc tgatcatgat tattaaaaaa tagagcattc
    11341 cagccgttac cctcactcat cttttctccc catgtatgaa atagtcgctt taattctgca
    11401 aaatcaaatg gaggagtaga ccatttattc ccattttcat aatcgacttt caaatgatgg
    11461 aaattaaaga ccattgataa ttcttctcta ctaggttctg tatacaagat acaattttcg
    11521 atcgtcgtag agctcatttc accgactgtg atgatagtgg aatccgtccc aaacgttgct
    11581 tggttcaatt cttttagata cgtatgggtg attggtttgt ccgtatattc aggtttgcca
    11641 tcattttctt tattattttt caattgttcg tctttaccga taacattgat aacatcgaaa
    11701 cggaatcctt ttaccccttt ttccatccaa aaacgaacaa ctttgaatag ttcctctctt
    11761 actgcttggt tccgccagtt caagtcagct tgtgtccgat catataagtg caaataatat
    11821 ttccccgtat ctccaaatgg cgaccaagca cttccaccaa attttgatac ccaatccgtt
    11881 ggctcttctc ttaaaatgaa gaagtcctga taatgctggt cccctgctag tgcttttttg
    11941 aaccattcat gttctgtcga aacgtgattc agcaccatat cgagcatgat ttctatccgg
    12001 tatttctttg cttcggctat tagctgttcg aattcctcca tcgtaccaaa gataggatcg
    12061 atggctgtgt aatcagagat atcataaccg ttgtcttttt gagggcttgg ataaataggg
    12121 tttagccaaa tcatgtcgat gcctagatca ttcagatacg ggagtttttc tttgattcct
    12181 tttaaatctc caattccatc accattactg tctttgtaag atttgggata gatttggtaa
    12241 atgactttgt ctttaaatgt catggatatt tacctccatt caactgattt attacctacc
    12301 acaacttata tatacaagtt atcatttatt attataactg aaccgaaagc gtttgtaaaa
    12361 tttatcttta tacattctaa gaggtctgtt gcccaataaa aaacaagaga tcagacatgt
    12421 atttgtctat cctcttgttt aaaatagcaa aatattcttg tttcttctat aaatggtgtt
    12481 caaaagcttt tatctacggc aatcagtctt ctcaaatgta aaacatgagg agctaatttt
    12541 atttgagtat tctttttgct cgatgcagga cggctttttt acaacctctg attcatggtg
    12601 ttcaatcagc tgtccttcgg tattagctca aaaaacggca aaatatgaaa aactattttg
    12661 ccgtttgttt ctctaatact caggtcaaac cgctgatttc acaacctctg tttttattca
    12721 ttcgatttca acgcatcaat catatcgata tgctttaatt tgaaatgggt aacaatcatc
    12781 acaataaccg taaatacgat agttaatcct gctgataaga cataagccca ccatgtgatc
    12841 actaatggaa agatgacatt ctccatagac gcttgttgca agatgaagtc agtcaatata
    12901 tatcctacac caaatcctcc gatgattcct aaaagagtaa agatgatatt ttctcgaaca
    12961 atgtacatcg tgacttcttt atcaaaaaat ccaaggactt ttattgtaga taattcgcgt
    13021 acacgctcgg aaatattgat attagttaga ttatacaaga cgataaaagc cagcagtcct
    13081 gataatacga caaagattaa gacaaccgag tctaagttcg ccattgattc ttcttgcgtt
    13141 tcgatttgtt tggacatgaa agacgtattt gtcacagcgt ctgtggcaag cagtttctcg
    13201 gaaagcattt cttcttcttt tgttgtcatc ttatctgtac gcagcaaaaa tgcattatcc
    13261 atcatcgatt gatcagtaac tgaagcataa tattcagggg acatataaat aaagctacct
    13321 agatagtttt tcacgatttc ttttacttcg actttccatt ctttttgatc ttcatcataa
    13381 agcgaaatat gatctcctgt agataagtca aatagttctg ctactttttg cgtaatagcg
    13441 attcctgttt taggcaagga gcgtgggttc tcttcaagat cagttaaatg gataaaacga
    13501 tccaaatcag ctgttttact tggtacgatc actgtcagac tttgcgaagc atgtcctttt
    13561 tttctcaatt ctacggtttg agtatgaata gctaggatct catttacttt ttcttgcttc
    13621 tgcagcatat ccgctgcttt ttgacgacta tcttccgtgg atgaatccaa tgtaacgatt
    13681 gcttgatagt caatgatcgg tccaaattgt ttggctgaaa cagaacttag agaatctttc
    13741 aatcccacgc ctgcaaccat cagcccggca catccagcaa ttccgataat cgccataagc
    13801 attcttgact tatatcggaa gagatttcgg taactgattt tttgattaaa gctaaggcgt
    13861 gaccacagcg gagtgatgta ttcaagaaaa atgcgtttcc ctggcttcgg tgctttcggt
    13921 tgaagcaatg cagcaggccg ttctcttaaa tctttgaata aaacgatcat tgcagcgcca
    13981 aatgcggcaa tgaagaatgc ggcagctgct tgtaaaatcg gcggccagtc ataaaaaata
    14041 agcgcactcc caatatcata ccgctcgtta gacagctcaa aaatgatcct tggcaaaaga
    14101 ttcgttccta atatcgttcc taaaacaata cctatccctg aggctagact tgcatagatt
    14161 gcatattttt ttgcgatttc aactttcgta taacctaacg cctttaatgt cccgatttcc
    14221 ctgcggtttt cttctaccat acgtgtcatt gtcgtaaagg tgatcaatgc ggcaataaag
    14281 aaaaagaaaa caggaaaaac attggcaatc gcagcgatac gatcagacag atcaccaaat
    14341 tcttgaaaac cgacattttc tgaacgttca ttgtataggt atgatggttt agttaaattt
    14401 tcaatttttt ctgcatttcg attcaattct tcctctgcag aggccagttc acgttctttc
    14461 tcaactaatt gcctttcttg ttcagcaagt tgagcttctt gttgagataa tgtttgaaca
    14521 gcgattgttc gttgcgcttc aggaagctgc tggatagttg ctgactgttg cgctaactgt
    14581 tttttagcat cttctaaccg tgttcttgct tgcgttgttt gtgctttgcc atccgctatt
    14641 ttttgctgtg ccggttctaa ttcttcgttg gccgattttt tcagctcttc taagcgttct
    14701 tcgggtcgat cttgtaaata cttttctgcc ttttcttgat tttgctccat tttatctttg
    14761 taagctttgc tgtacgtatt caaattcttt acgtttgtaa aatcaagata taagacagaa
    14821 taaatatctg ttttaaaaac ggattcaggt aaatagacaa aataatcgac gctaccattt
    14881 ccgacgttcg cgtatcctct ctccaaattg ttgatgaaca atggagaacg gacaaagccc
    14941 gtgatggtat actctttttc tttcaattca tctgaatcaa tctgataaat atcaccgatt
    15001 ttataccctt catcttccgc tttttcatcc aatacaagct ggtttggttt ttccgggagt
    15061 tttccatcag ttaattgtag cttgttctgc ttgttatttt tgttataaga aaatatttga
    15121 atcacttcgt ttttgccagt attggaataa tagatctgtt ttcctgtttc tacttttgca
    15181 tctgggatct tttctgcttg agcaatatct ttttgtgtca agccaccagt agaaacgatt
    15241 tgcaaatcag aaagtcctgc tgtaccaaca tagtgatcca tcgttttttg catgacaggt
    15301 ccagcagttt tgacacctac aaataaaagt gttcctagca tgacaataag gatgattgca
    15361 atgaatcgtc ctttagattg ccgaatttcg cgaaaacctg ctttaagata tgttcttgtt
    15421 ttcataaaat ccccctacca tacaatctca tcaatggatg aaggatgatc atttaattca
    15481 acagacctta ctttagcatc attaatgtga atgactctat cagcgatggg cgctaaagca
    15541 gaattatgtg taatgatcaa aacggtattg ccgtgttgtc tgctagcgtt ttgcaataat
    15601 ttcaatactt gcttgcccgt ttcaaaatcc agcgcgcctg tcggttcatc acataaaaga
    15661 agtttgggat ttttagctag tgcacgagca attgaaaccc tctgctgttc gcctcctgat
    15721 aattgggacg gaaagttatt caatcgatga gctaggccca cttgcctcaa tacctctaca
    15781 ggatcaagcg catcaggaga aacttccgtt gccaattcca cattttcttt tgctgttaga
    15841 ttaggaacga gattgtagaa ttggaaaacg aaccctacat ctgttcgtcg gtaagcagta
    15901 agctgtttat ctgaaaattg cgcaatatct gtgtcatcaa taatgatctg accttcatca
    15961 ggtgtatcca tcccacctaa aatattaaga atcgttgact ttcctgctcc acttggacca
    16021 agtataatca ctaattcgcc tttttcaatt gaaaaagaaa tatcgtcatt tgctgtaatg
    16081 gtcgtttctc ccattttgta tcgtttgtat tcatttttta cttcgacata cttcactctg
    16141 attcctccat tttcacgata tttctcgcta tatctatgca tcgaatgcgt tttctcacac
    16201 ttatttattt taccactgaa tttctctaga ctaaagaaag gaatccttta atttagttga
    16261 caaataaacg atttatgatt tacttacata aaaaacattg ttatttgaaa gaaggaacat
    16321 catgaatgat ttaaaggaaa atatgatttt tgatttatca aaaactgctt ttgtcgcgat
    16381 cgacatgcaa aaagagatcg taaataacgg tacactctct ccacacactg cagcatctat
    16441 tctgacaaac aatgatttgt tagtaagaac attgaaacgt accgctgcct tgaaagtttt
    16501 agtgaacgta gacatctcca ctttccctta tttgagtcaa caaactgata tgggtggaat
    16561 gactgcttct gtacctccag aattcactga tttacttttg aaggattctt tgaaagatac
    16621 agataatatg ttgactatca caaaatacaa tccaagtgct ttttttggta cttctcttga
    16681 tcttcaactt cgccggcgtg gaatcgaaac tatcatttta agcggtgtag cgacaacaaa
    16741 cggtgtatat gctactgcat tagatgcctt tcaacacggg taccatatcg tgcttgctga
    16801 agatgcttgc agtgatcgtg acaaagaatc ccatcaattg ttcataaaaa agatatttcc
    16861 gaaaactgca cgagtacgct caacgaaaca aataatcgaa gctatccaac aaagcaaata
    16921 agtactggca cacgtgattt tcatttactt ctttttgatt tcatgggaaa ataaccacag
    16981 gatttagata ggagtatgaa catggtgaaa aaaaagatca caatcaaaga agtcgctaaa
    17041 ctttccggcg tttcaatcac aactgtctcc ttgatattaa atggaaatac ggagaaattc
    17101 agtccgaaaa ctgttcagaa agttttagct gccaaagaag aattaggcta tcagcctgac
    17161 tattttgcta gacaaatgat taccaaggaa acgaaaacaa ttggtgtact cgttcctgat
    17221 atcacgaatc cattcttcag tacactgatg cgaggaattg aggatatctt gtataagcag
    17281 aatttcgtca cgatattatg taatgctgat tctgatcatc aaaaagaaat cgaatatcta
    17341 gccgagctca cacgtcgagg tgtggatgga ttcattatcg cgacttctgc tgtctctaca
    17401 gatgcaatca acgaaaattt gaaaaaacaa ggccggccct tcattgtttt ggatcagaaa
    17461 aaatctgaag gatttagtga tgccgtacga acagacgatt ttcgcggtgg ttacctagcg
    17521 ggcatgcact tgcttagtct aggacatcaa acgatcgccc ttgtctatcc tgaaaatcca
    17581 ccggaaaatg tccacgctcg gattgagggg tttaaatctg ctcttgatgt ttaccaaatc
    17641 cctcacgacc aactgatcct cttacctaca cagttctcaa aacaaggtgg ctaccagatc
    17701 acagcagaac ttcttgattc tgctgcaact ggtgtgtttg ctttaaatga tgaattagct
    17761 tttggattat atcgcggatt agaggaagct ggaaaaagca tccctgaaga ctacagcatt
    17821 attggctatg ataatataga tatgtgcgag tatatcaagc cgaaactgac aacgatcgca
    17881 caaccaatct tcgaattggg acaaacatct gccaagttat tgttagatcg gatccaattc
    17941 ccagaaaaag aatgggaaga aaaaagatta cctgtacgat tagaaaaaag attttcaact
    18001 gcacccttga aatagctttc aaggtgtgct atatttttat cgaactatta aaacgtttta
    18061 ctaaaacgtt ttaaaaaata attttcccat caaaataaat gagatagttg gaggtttgta
    18121 aaatgaacac ggttacagta attggaagta ttaatatgga cactaccctt cgtttaacca
    18181 acatgcccaa gccaggcgaa acgatgcatg cgcatgagat ttttcacgct ggcggaggta
    18241 aaggtgccaa ccaagcagtg gcagccaaac gttcaggcgc acggacatct tttattggag
    18301 gcgttggggc tgacagcgaa ggacaacagt tattagatct actgacaaaa gaaaacatcg
    18361 atacatctgg gatagctgaa atccaaggcg ccacaaccgg acaagcaatg attatggtag
    18421 acgcagcagg agaaaatagt atcttgatcc atgcaggggc aaataatgct ttccatgaac
    18481 aagaagtcct aaaaaacaag caactgatta caaacagtga ctttattatt gctcagtttg
    18541 aaagtagctt agatgcgacg atcctagctt tttctattgc aaaagacgca ggaaaaacaa
    18601 cgatcctgaa tcctgcacca gctcgtgaaa cgattcctac tgaactactt gaaaagacag
    18661 atatcattat accaaatgaa acagaaacag aaatcattac aggaatcaga gtgacagatc
    18721 ataacagttt ggtcgcagca gccgaaaaac tccatgaatt aggtattgga acagtaatca
    18781 ttacactagg cagtgccggc gctttctatc atacagaaaa agaacacggt atcgtccctg
    18841 cttttaaagt ggatgccgtt gacacaacag ccgcaggtga tacatttatt ggcgcattaa
    18901 gcagcacact tcagccagac cttagtaact tgaaagaagc gatcctttat ggaaatctag
    18961 cctcttctgt tgctgtgcag tcttatggtg cgcaaccttc tatcccttac cgcgaagctt
    19021 tagaacaatc agcagtctaa aattaacaat gagaggaaga aacaaacatg aaaaaaggaa
    19081 aagtaatcaa ttcagatatt tcacgagtaa tcgcacaaat ggggcacttt gataaattaa
    19141 gtatcgggga tgcaggcatg cctgtcccct ttggtactga aaaaatagat ttggctgtct
    19201 caaacggtct tccaagtttt ttatctgttt tagaaaacgt tttagaagaa cttgaggtac
    19261 aatcgattga attggcggaa gaaataaaga caatgaaccc ggaaatattg gaaaaaatca
    19321 aattattgtt accagataca ccgattagct ttatcccaca tgaagaaatg aaaaaagagt
    19381 tgaataactg tcatgcattt attcgtacag gcgaaatgac accttacgcc aatattatat
    19441 tagtgagtgg agtggtcttc taatggaaac gacaatgaat cctgaacaga tggtactgtc
    19501 cgagcagatt catatgctga ttgaacagtt gggaggaaaa gacaatattg attctgtcct
    19561 tccatgtgcg actcggcttc gtatcaaatt gaaaaaacag ccagtagcca atgcagaaaa
    19621 attaaaacag caaatccctt ccattaagta tttcatttta ctcgatagat atgcagaaat
    19681 aatcatggat cagggattgg ctcctgctta caaggaattg acgcgtcaaa tcgatcattc
    19741 agctccgaaa gaaaagctaa atccgatcga aaaactattt ggtttgatat cggaaatctt
    19801 tacgccgatc attccgctgt ttgccggttc tggtatatta aaaggtctgg ttgttttagc
    19861 gactacgatt ggctggctta gtgaaacaag cggaacttat catattttgt ctgctgcgtc
    19921 gaatgccgtc ttttattttc tgccaatctt tcttgctttt tctgctgcca aaacgtttaa
    19981 agtgaatgga tatattgcgg ctgtcattgc tgcagcactg attgaaccga atctgacctc
    20041 tttactttca gacagtggca aagcagctac tgattttctg gggattcctg tagtactgat
    20101 gcagtatacg tctactgtca tgccggcgat tttaggtatt ttcttttatt cttttgttga
    20161 aaaattcctc aaaaaatata ttcgcgaaaa tatgcaatta gtatttgtac ctttactttc
    20221 attagttatc acggttcctt tgacgttaat ggtattcggg cctgcgggtg tttaccttgg
    20281 cgaaggttta gctgccgcaa ttacttggat gatggatatt aacggaccga tcagtggcgc
    20341 aatcatcgct ggtggttgga atattttggt aatatttggt atccaatggg ctgtaaaccc
    20401 tgtcatgatc agtaatatct ctgcttatgg gttcgaccga atcgttccgt tgacaggagc
    20461 agccaatttt ggaatggctg gtgcagcttt aggtgtattt ctgcgaagta aacgttcaaa
    20521 aacccgttcg atctccggtt ctgcttttgc ttcgatttta cttgcgggag tgaccgaacc
    20581 aactgtttac ggaattgcga tcccattgaa aaaaccattt gttgctgctt gcattggtgc
    20641 agctgctggt ggagctgtaa tgggatttgc ccaagtgaaa gcgatcgctt ttgtatttgg
    20701 tagtctgacc acattgcctg cattcatttc cggtactttt ttctggtact tagcaggact
    20761 ggctgttagt ctagtcgtag caatgatcac tactctcgtt agcgggtttg atgaagacct
    20821 aatgtcttat gaatagttaa tgaaaaagac tgaagtatga gatgtttatc acatatttca
    20881 gtctttttct ttttaggatc attattttta taaatttttg tgatactttc caaactttgg
    20941 tataatttac atatgcataa ccatttttat ccccacttgt ttatccccaa acaaaggtgg
    21001 tctatgcaat aaaatgaacg tcaaagaaaa acgaatgaat gctcgttgct tatttacaaa
    21061 ttgttttatt tagaatacac agacagattt atgaaaagaa aaagcttttc gacacgatta
    21121 tctgttccaa aaaatgtcta atgaatgctg aataagacag aaaatcaatg aaaggaaaag
    21181 gactagttta ttatgaaaaa taaaaaaacg atacgtttgc tagctgctac ttggctgatt
    21241 gctccgtctg ttttgtcgac caataccgta tttgccgtag ataatcaaat agctgatcaa
    21301 acagtcacaa cagatacgac tacatccagc tcagctacta gtgaaacaac ggattctttt
    21361 acagaagacc caactggaac aactaactca acaacaggat cgaccgagcc aactgaaatt
    21421 gatccggctg atccatcaga aaatgaaaag ccagccgaga catattccct tacaattgct
    21481 ccttctttga ttggtaagat ccaactgact gcttcgacag cggattcccc tgaacagcaa
    21541 cctttggata ttacggaaag tggaacggta gaaggtttat tagcggaaac gaaggtaact
    21601 tacactatta cgccagctga aggtgaacaa attgcttcag tcgctgttaa tggtgccaaa
    21661 gaagtaaatt atgatgcaaa gacctttaca gtaggtacga acaatttatc tattactgca
    21721 gaatttaaag ctggaccgga tacttcaaca gagaaaccaa ctgatcctag tgaagaagtc
    21781 cctagtacac cagatccgga aacgccaaac aaaccagatg tgggtaataa caacaataat
    21841 aatggttcaa ataattcaaa caataataac aataattcga acagcgggaa ttcttcgaat
    21901 caaacaaacc catctggatc gaataacaat ggaaatactc agagagaacc aaaccatcca
    21961 aacaatggtt catcgaatgg cggaacgaac aatgcttcat ctcgtccaaa tacagcaaat
    22021 caaagcggta tagagaatcc atcttctgct tcttctgatt ttgttgtaaa atcaccaatt
    22081 gatgctgtat tgcctacaaa tacaacgaat gtccaacaag caatcgttcg agaagcattc
    22141 aagcatcttg gaaaaccata tgtatgggga gcaaaaggtc caaatacttt tgactgttct
    22201 ggtttgactt attacgtata tatgaaagca accggtcatt atatcggtgg ctggacaggc
    22261 gaacagcaat atgcaggtac gcagattcca gtaagtcagg cccaacctgg cgacctcgtc
    22321 ttctggggac cttcttccgg tgttactcac catgtaggta tctatattgg gaacggacaa
    22381 tttatccatg cgcctcaacc aggagataaa gttcgagtaa cttctatttc agatttcacg
    22441 cctgattttg ctgtccgagt aaacttagca ggtttacctg ctgcttcagg ttcgctgaca
    22501 ggcggctcat ctattttaga tggattagat ggctcgttcc atttcagtca aaaccaaaca
    22561 actgatcagt tcttgaaaaa gattgcagat gatgcacagg aaatcgggca aaaagaagga
    22621 atctacgcat cagtcatgat ggctcaagct atccttgaaa gtggatctgg aaacagctta
    22681 ttatcaagcg aaccaaatca taaccttttt ggtatcaaag gatcatataa aggaagcagc
    22741 gtgaccttca atacacttga acaagatagt tccggacaaa gctaccaaat ccgcgcacaa
    22801 ttccgtaaat atccttctta caaggaaagt ttggaagact atgccgactt gatcaaaaat
    22861 ggactgactg gcaatcctga tttctacaaa ccaacttgga agtcggaaac aaaagactac
    22921 aaagaagcaa ccaaatattt ggaaggacgt tatgctacag atcgccaata ttcacaaaaa
    22981 ttgaacgcga tcatcgaagc gtacgacctg acaaaatatg atgaaccgaa aaaagaagca
    23041 gcaacaactg aagaagcaaa cgctgaaatc ctcaatatgt ctaagcgatt cgatgtgcca
    23101 atcaaatgga caacagatgg cgtgccaatc acaggattag aattttcaac gataaaacgc
    23161 gtcaacaacg cttcttccat cttccgtgtc gcttcaatct gggatttgtg gaacaatttt
    23221 acggcaagac agataccaga aacaactact cgtacagtta aagctgcaac tgttcctgta
    23281 ctaaccttgt taagtttaga caaatcattc tggcttgatc tgcctttaaa gaaatagaat
    23341 aaattaaaaa ggggatgtga cactgtcaca ctcccttttt aatttattct atttctttat
    23401 atttattttg tattccgtta caaactcgcg tacttggtta ggttccagag tgacatctcc
    23461 aaaactttca agtcgctccg ctcctggcag ttcttgagtc tcaaatgtga taccgccatg
    23521 atttactaat ttattcccgc gcatttccgg tccatcttct ccaaaatttg ctgtaaaaat
    23581 cacgattcct gaagcatctg tttttacttg gatcgatact ttttcatctg ggcttgtcaa
    23641 ttccgcagca tttagtaaac cagattgttt tttcaacaaa aatggatgat cgatgccgcc
    23701 cactatcatt gattggcgaa aatcactggc aaagacctca cctaattttt taggacgttg
    23761 gaagtcaaat gctgtgccac ttactgaaac tacttctcct gtaggcgttg tatctttatt
    23821 gagcgtagca aataatcggc tattcaacca aagtatatgt tcgtcgattg actgtgctgg
    23881 atcaccggtt aaattgaaat agacatgatt tgtaggattg aatactgttt tgcggtcact
    23941 gatcccttgg accgtcactc gccatgtatc atcgttcgtc agcgtatacc gtacttctac
    24001 atctaaagtt ccaggaaatc cgttttccat atctgggcta gtctgataga aaatgaccga
    24061 tgcttcgttt tcaccattga tgactgtata atgccactgc ttggcttcaa agctaggtgc
    24121 accaccatgt aaacaatgac cagtgtccgg gtctgttttt acttcgaacg tttcatcatc
    24181 taatttgaat gtgccattcg taatccgtcc agccatacgt ccgattgaag caccgatata
    24241 agggtctttt tctagatatt cttttgcttc atcaaaacct aagatcaact ctcgtttttc
    24301 attatcgatt attgtttgaa aatgaacaac ccgtgcccca agatcactga cagacaaaga
    24361 tgtcccattt tgattcgtga tcgtaatcaa gtgatagttt tcgccaaaat tttgttcatt
    24421 gatttccatg ttccttgcac cctcttttct ttcatttgca ctgattccag tatacatgga
    24481 aaccgttttc ttaacagctt attccgtgag taacgagaaa aaatttacta tattctaata
    24541 aaagaccgtg aaaaaaaggt tgtgacaaaa atattgtcac aacctctttt tccgaacaaa
    24601 cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc aaacgaaaaa tatgaggagc
    24661 tatttttgct tggctgttct tattacttga tgcaggacag ctttttcaca atctcttttt
    24721 caatacgatt ccagtatata accatgtcgt cagtacatag tagatgaaat acaatacgaa
    24781 gaatatcgta aacggaaccc aaatatcttt gtatggattt tgcatgaata gaccaaacat
    24841 ttgtaatccg aataagacat ggactacacc taagattcct ggtgctaaga ataacacgcc
    24901 tacttctcta cggatcgatt gtttaagcaa cccgcgtcgt gttcctatct tttcaagcat
    24961 ttcataacga gcaatgtcac tcttgcttcc tgacaagatc ttgaacatca aacagctagc
    25021 aagcatggtc aagaaagcta aacctaggaa gaatcccata aattcaaaac cagagaatag
    25081 accattatac acttcataca tttcatattt ctggttgctg tactctactt tttgcaaaga
    25141 tggattgttc attttatttt gagcaacaag tgtttcgatt ggtttcaggt ttgcttggaa
    25201 gtcttttacc tgaactagct gtaaagtgga ttgtggcaag ttcagttgat caaattctga
    25261 ttgactgact accttgattt ctttttcttg ctgttccggc aattcatagc ttcgaagttc
    25321 ctcttgtaca gtcagttcat tcattttttt agcaggaact ttttctttcg tgatcttcgg
    25381 cggtgttcca tcttcatgtt ttatcatgat caatggttga gccgcaaatt cacttgcatt
    25441 atagtaaatc gttcccgcat cttctttttg agtataagaa gcagaaagtg ttggtgaaag
    25501 ttctttgatt ttttgctgat cttctgcccg aggattattc atcactagat catatccaga
    25561 attgttttct gtcaagtctg caatttcatt tttaaaacct aatcctaccg tcaaagcgcc
    25621 cagtgccaag gcaaacagca tagcgaccat tgagagcatt tgtgtatatt cacgaatacg
    25681 aaagtttaac tgtgacaatg tgaaattgtt caattttttc aatgaaatct gttctgattt
    25741 ctttagtaat gataaaaaga aaatgatcac agaatggaaa atgaaataag ttcctaatac
    25801 gatcgtaact aaggctaccc ctataccaaa gacttgatag gtccccacgt gtttcatcat
    25861 atagtaaccg cagcctagaa aagcaattcc cagtactact tcaaggaaaa acaagaattt
    25921 atgctgcttg attcttgttg gtgtctgatc cgcacgaatc aatgctaaaa tcggtttttt
    25981 gacaatcgaa aaagcattga caacagcagc taataaaaac aaaacagcaa aaaacaaaac
    26041 agtgatcaac aacccgtgaa tactaaaagg tgtaaaatgc gaaatattga gatctaactg
    26101 atccactaat aatcggtgga cgatacttgt caatcctaca ccgacagctg aacccactaa
    26161 agtagctgcg atccctacta agaatgtttc agaaaaaatc atctgtgcga tttttctgcc
    26221 ttttgctccc agcatcataa acattgcata gtctttttgc cgcatcgtca tcaaaaagga
    26281 atttgcatat aaaatataaa cgaatgtgat gatccctagt aaaacagaac cgaatcggaa
    26341 aatgataact gtagccgcaa cgatcgtatt acttttcaaa aaagcttcgt tgcttgccat
    26401 tgattcaaac atgtaaaaaa tagcagatgc cataactaat cctgagaata acaccaagta
    26461 gtctcttaaa cggctcttga tcccagtaag tgataatttc caaagcatag tcaatctccc
    26521 cctactgttc caagtttcca agaatagata gtatttctcg atagaaggaa tcacggcttt
    26581 gatctccacg tttcacttct tgatggatac cgccatcctt gatgaataaa atacgttggc
    26641 aataacttgc agaaaacgga tcatgcgtca caagaagaat cgatacatga tcttttgtat
    26701 tcaattcgtc catcgtatct aaaagatccc gggcactttt ggaatccagc gcacctgtag
    26761 gctcatcccc taatagaatc gttggttgtg tgatcaatgc acgtgcggcg gcgacccgtt
    26821 gtttttgtcc accggaaatc tcagaaggat agctttccaa aatatgagag atggataaac
    26881 gttctgcaat tttttggaca cgttgcttga tttcttttgg tctgacgcct tgtaaagaaa
    26941 gaggaacagc gatattttct tgtgctgtca agctttctaa taaattgaaa tcttggaaaa
    27001 taaagccaat ctcattcgca cgaaaatctg ctagttgatt tccttttaat gtggtcacat
    27061 ctttttgatt gattcggata tgcccatctg ttggtttatc taatgtggat aaaatattca
    27121 aaagtgtcga cttaccagaa ccagaagcgc ccatgatccc aataaattcg cctttttcta
    27181 catcgaagct gatgccgctt aatgcttgcg tctttttctc attgtttttt ccgtatactt
    27241 ttgttacatt ctgtacttcg actattttgt tcatttcttt ccctaccctt taattagttt
    27301 ttccagtgct gttttcggca cgtctttttt ttcgacttct tcatacgaag tgacaccttt
    27361 atttttgttc caggtgattt ttaaatatgc tcctttacga agcggtcgag tcaattgtcc
    27421 cttgaagtcc agttcttttt ccttaccctc tttcgtatag ccaggcagga tatatctata
    27481 gttgactgtt tcttgtccag agtctgattt tgaaactgtt tttgtaccat ctgtcgtaat
    27541 ttgtacatag tattcttctc cacccataac tacgtaatct gcgattttca cccctgcaag
    27601 taccacaacg atgaaaacaa gtagaccgat cagaagtttt ttcacttttt ccccttctct
    27661 cttactttat atactgatta tagaaaaaag cgaacaattc tgccttcgaa tcaactatca
    27721 ttcttctttc aattttgtca ttttctttaa aacaactaaa aaaagagttc aagacaataa
    27781 tccgtcctaa actccttctt cctatcttac aaaaagtatg tcacttacgg tctttcatgt
    27841 cgcctgtatt gataacaggt tgcgttcctt tgtctgtaat gattgagaca agtaaattat
    27901 tgatcaattg gactttccga tcatcggtga aattgatttc ttgtccatct tcgatttgct
    27961 ctaaggccat ttgagtgatt gtgacagctc cttcaacaat ggtttgtcga gcagacaaaa
    28021 tggcttttgc ttgttgacgt tggagcatgg cactagcgat ttctgttgca taggctaaat
    28081 ggttcaagcg tgtttcgatt acttcgacac ctgctactgc taaacgctct tgcagctctt
    28141 tttgcaactc gtcagacact gccgtcgtgt ttcctcgaag cgtcaaatca ttctcattga
    28201 atgtatcata cgggtattgg ctagcgatat gccgaatggc tgtctcactt tggatttcta
    28261 caaaatcttg ataatacgct acatcgaata atgcctttgc tgtatcaacg actttaaaga
    28321 ctactactgc tgagatttcg atggggtttc catccaaatc attgactttt aaaacagaac
    28381 tattgaaatt ccgaactttc aacgaaacag tcattttttg tgccaaaggt atcgtaagga
    28441 aaaagccatt ttctcgaatc gtacctaaat attgtccaaa aaacaaaatt acttttgctt
    28501 gattaggact gactacagtt gccgaactta acaacaataa cgtgatcaac caaagaaaaa
    28561 tacttaaaaa aagtgcccaa actttcgctt gccacatacc taaatagaag aaaaacaatc
    28621 caactaaagc taatacgacc aatccgacaa tccctgcata accattgata taaaatgctc
    28681 ttttttctct catgctactt cctgcgctcc tttatccttc aataaatatc taatgttact
    28741 tttttaattt ttttttcgaa tacgcgccat tacaccttct agttctaact ctttgtcttc
    28801 agtgatgtac caaccgcttg cctgtgtccc tccggctgcc tgcggtaaat aatccaattt
    28861 tgttcgaacc cagtgctttt cttctgtaaa atagtcttct accaagacct ctagatccat
    28921 ccctgctgaa agaggcaaat cttcccgaac agatttaaag ataaatcgtt ccttcttccc
    28981 atcatacttc aatattcctg tatcgataat cggtaaagaa taataatcca gctctttctt
    29041 caccgtcaat aaatgtttga aaacttcatg gtacacttgc tctaattggc gaccttctga
    29101 attgttcggg taattaacca tcactttttc tttgtctgga tcatagaagg tttggcttag
    29161 ttgagtgata taagtatgta atctttccat ttctgttttt aacttttctc tttgaccttc
    29221 ttctaccttc atatactctt tcaccctgtt ttctttttag atattttctt tcttatttta
    29281 ctatagttat gtgcgttaag cgatgaaaaa cagcaaagat tggaaggatc tttgctgttt
    29341 tttcttaaaa tatccagttg agtacaggat caatctgttt cattgtcctc ttgatccgtc
    29401 gtaatattga tcgatgtgtc tttagcagtg atctttagct ttttctgtcc ttgttctaac
    29461 aaagtatttg ttattggctg gtagtctttt tcaatataag attcaccaga cgtttggata
    29521 gtatagccac tgttttccat ctcatgcaaa gataaattgc caccgtttag gatgactttt
    29581 ccttctttgc tgatcaaact gtcttctaga tggaagttgc tgttttcacc tgtgacgatc
    29641 aagttatcca cattggcctc atctaatcga agtgagccta attcatcttt cgcagttatt
    29701 gttcctcctg taaattcatt gaacgatacc cagccatcat gtgtcttagt attcactttt
    29761 ttgattgata cttcgttgat atcaattgca ctattattcc cttcgattgt caattcagat
    29821 aattcatgat ctgcagggac aacaatagtg atttgtgcac ttgtgtcttc taaactaatg
    29881 attgcacctg ttgatttttt gactgcattc acagtcaaca cgccattttt ctcctttatt
    29941 tctggctttt ctgtcttatc ataagtcgtt tttatctcaa aatccaatcc gcgtatgacc
    30001 gaaactgttt gattttctgc atggatgacg attttgtttg catccttgat tttttttgtc
    30061 tcagtaacca tatcgatcaa ccgaggacca ttatcccaag tcagactctt ttgagcgcct
    30121 aatgtataag ttgcacctgc taggaggcca ccagtaagca tcagaccaat accggtaatc
    30181 aaaaaatgtt tcaatctcaa tttatgtgtc ctccttcaaa cgcccgctga atgcagatct
    30241 ctttttgtaa taaaacgtcg tcccatccat ttaacaaagt tcatcatgac ataaaaaagc
    30301 ccttttgttc ctttcagcaa cagtggcgaa ataaatactg cgcttcctgt ggcgaacaga
    30361 ccgacaccga taaagaataa tccactcaga atcgattgtc caaacacact gattcctgag
    30421 atgatcatga ataggccaac acctaagata ctgatacata aaacatagat actaaaaatg
    30481 agcgctagta aagcaaccaa tagtccccca ataaccaata taaaccccag tgctagtgga
    30541 atgaggacag gagaagcaaa taaaccgata atgattaacc aaatcaggcg aatttgtctt
    30601 tttggatttt tcactaactc gttttctgct tgttcttctt ctgaaaagta actgattttt
    30661 aattttaagg caaactgctt tggtgtacca tattcatcaa tgatctgatc taatgttttt
    30721 ccagaatcta aaaaatattc ctcataatac tggatcaaat caaaacgatc gtcttcagga
    30781 atttcttgaa gatgatccac aacttcattc aaatattcat tcatttgtgc tctcctccaa
    30841 taaccggtct aaagaatctc gaaaaacaat ccattcatca ataatttctt ctaaatgacg
    30901 acttcccatt ggtgtcaacg agtaatatcg acggtttctg ccttcaaaag gttcatcata
    30961 ggtctcaaga tatccttctt ttttcaatct tctcaaaacc ggatatagtg tagattcact
    31021 gacagagaga ctttcttgta cgatttttgt taaggcatac ccatacatat cttccttgct
    31081 taatgctgct agcacagtac catcaagaag aacagttgga atctggatgt gcattacgtc
    31141 accttctatt ctcaagtgta atattttatt tcaatctata ttatacaaca tataatatag
    31201 tttcaaaatc agtttgtcaa atacaaaaaa actgactaaa agatttgatc ctttttagtc
    31261 agcaaaaaat ggcttctctt tgatttttat tataacagtg gcataatcca atttgtccac
    31321 atccagctca gtggcatgat aacgatcatt gcaggtatca tgtcagctac tgggaacatt
    31381 ttcacgttga tcattctaaa accggtagcg accatcaaga atccgccgca agctttaaaa
    31441 tctaagatca tgtctggtgt agtcaatgga taaataaatt ttgccaataa gaacaaaata
    31501 taaaaaataa tgaattgggg aattgctatc acagaaacga cataccctaa attacacgca
    31561 aaaatagctg ctgtgaaaaa atcaaggaca gatttagaaa tcaaaatcgt actgtctcct
    31621 gtcatcccag aatctaaact tccatagatc cccgtaccac ttgcacaaaa tagaacaatg
    31681 accgtcacta atgtgctgat aaattcatcg tgggtcatac caagcttttc actagggaag
    31741 attttagaaa taggtacttg catcagcgca gcccccttat ttatcccatt acctaaatga
    31801 acaatcaaac caatcgctgt tcctatcact aaagcaaaaa taacagccgg catatttttc
    31861 atcggcgcaa tggaatagat tcccatcccc atcgaacaca caccaaaaac catcgtcagc
    31921 tgtgctttaa aatgttcact taatttattt cctactaatc cgccaattag tccgccaaac
    31981 aaaacggaac ctacattgat caaaactcca gttggcatca aaaaactcct ctctttttct
    32041 acgaagcgaa aaaaatcaga aaaatttctg taattattgc tttcgctgca agaaagattg
    32101 taacagacag agaagattac accaattcat tttttgtgtt aaagtattct ttattggaat
    32161 aagtaggagg aatatgatgg atatccataa attcgaagtt tttcttgatt tagccgaaac
    32221 aatgaatttc accaaaactg ctgatcgaca attcaccacc caaggaaata tttctaaaca
    32281 gattctttcg ttagaaacag aattagacgt caaattattc gaacgcgccc atagaaaaat
    32341 cgaattgacc gaagctggaa gcttattgct cccatatgta aaaaatgtcg tagaacaata
    32401 ccatgctatg caagaagtgc ttgaaaccta tacgaaagac aacaatcttt cattaaatat
    32461 cttaacgata ccgacaatga tcaattataa aggtttttcc aaaatcaccg agtttttgaa
    32521 acgccatcct gaatttacag tccagttgaa agaagtggaa agtgtcgaat taacttacaa
    32581 tgacgccaat gaacatagtg acacgatcca ctttgctaga agtttccagg ccccaccaag
    32641 ttcgattgag tggttgccta ctgaaacaga cgattttgta gcagttctac ctaaaaatca
    32701 tcctttagct agtaaaaaga aattggatct aagcgaactg aaaaaggaga actttttgct
    32761 ccttggcccc actacgaact tgtatcaacc tgtactagat ctgtgtcgcg aagctggatt
    32821 tgaaccaaaa atctcttata aaggagcaag aatcgacttg atcatcaata tgattgcaaa
    32881 tgacatgggg attgctattg ttatggaaaa aacagtcaag aatctattaa aagaaaatac
    32941 tgtgattctc cctatttctc caacaacaga gagttatctt gcatttactc gtaaagtcgg
    33001 tgagcattct ttggcttcag atacattttg ggagtattta aatcaagaag aaaactaagc
    33061 cctacatttt ctgtccatct atccacccaa aaaaagagag cgacgcatga ctttcgctct
    33121 ctttcttgca tcatgtcctt gtttcaatga cacctctaac atttctcgta gttcaataag
    33181 aaagcagcga tttttatgcc aaaagaaccg taagaacatt tccctaatca caaaaaaacg
    33241 actaaaaacc cctaaaaacc ttcaatagaa tttttagggg ttttattctt tttcctattg
    33301 tttacttaac cgtatcattg ctgtcgttcc tactccttcg atactgctat aatcaatacg
    33361 tccatcatgc agttctagaa tttttttaac cagtgaaagt cctagcccat tcccagaaat
    33421 ttgacgactt gtatctcctt gataaaattt ttcaaacatt cgatctctgg tctcttcatt
    33481 cattcctaca cctgaatcgg tcacttccag tacaatctct gtggctgtct caaatagttt
    33541 gatatgaatc tgtccattga tttgattgta ctttatggcg ttatccatga tattcagcca
    33601 cacttggtac aaaaactctt cattgcctgt gtaatttact gcagcaagtt cgatatccaa
    33661 ttctagctgt tctttctccc atttaggctg aaaaaaaacg attacttctc tgatttgttc
    33721 atctaatcga tactctttct tttccagttg gatacgctgg ttttccagtt tgttcaattt
    33781 taagatgttc tctgttaatt gtgaaagctg cgtgatactt tcgataatcc gatataaaaa
    33841 tatttggcgt tcttcgtcag agagatttgg ggcttgtaac aattgaacat atccttggat
    33901 tgttgctagt ggtgttttga attcatggga tacactggac acaaagtcat tcctcagtgt
    33961 ctcgatacta ttgagttctt ggaccataac gttgaaatct ttatacagtt gctgtacttc
    34021 tgctactttc tgctgttcat ccattcgaat cgaaaaatct cccgttgcta ccagactcat
    34081 atttgtccct agcgcactga ttggatgcag gatacgccgc ccaacaaaaa tcgatattcc
    34141 tgtacccacg accatactga accccgcaaa gacgaataac gggtataacg ttggttttcc
    34201 agccgattca gcaggcgaga tagaatgtcg agacaataag aaaataaaac cgagaaaaca
    34261 cagcagtgtc actaagatcg aaatgaagac aatactagca aaatagatcc ataattgtga
    34321 aaatagcttt tttttcatct ttaaataacc gccttatagc ctagtccccg tactgtgatg
    34381 atttgaaaat cttccgtctt ttcaaaacgg gtccttaaac gtttgatatg tacatcgatc
    34441 gttcgttctt ctgtattcgt atctaatccc caaatatcat ccatcaactg ttggcgagtg
    34501 aagatcttgt tgggataaga cagtaattta tacagaagca gaaattcttt ttgaggcaga
    34561 atcaacgaat gctgatcttg taacactgta taagcatcta gctctaattt tgtttgtccg
    34621 attatcaagc tactgctgta attgatcttt gaacgtcgaa gcaatgcttc cacacgtagg
    34681 atcatctcat tgacatcgat gggttttgtc atataatcat ctgctcctaa tttgaacccg
    34741 cgtttcttat cctcaaaatc ggcctttgcc gtaatcatta gaacaggtgt gtcaaatctg
    34801 gtgctacgta atgagtctag caagtcatag ccatccattt tcggcatcat gatatctgta
    34861 atgattaatt ctatacgtgt cgcttctagt tttttcagcg catcctctcc attcaatgcg
    34921 gtgatgactg aaaagcccgc tcgttccaac acagaacggt aaagcaactg taattgcgga
    34981 tcatcttcta ctactaaaat acgattcatt gatctccctc tgttttttga aaaatttcta
    35041 gattttttct tgtagttcat cttcagttaa catttatttc gtatactacg atcattgttc
    35101 tgatatactt attagtatac cgaatatttc aatacgggag aagggtgatt atgcttcaat
    35161 taaaagatat aaaaaaatat tataaagtcg gcgaaaccac caccaaagca ttggatggtg
    35221 tgtctgtcgc tttcagacaa aaagaattcg tagcgatttt aggtcctagt ggttctggta
    35281 aaacgactat gctgaatgtg attggtggac tagataatta tgattccggt gatatggtaa
    35341 tcaacggaaa gtctactaaa gactttaagg acagtgactg ggatgcctac cgaaacaact
    35401 ctatcggttt catttttcaa agttacaact tgattggtca tctaggcatt attgaaaacg
    35461 ttgaacttgg gatgacatta agtggtgttt ctaaagacga aaaacgaaaa cgggcagaag
    35521 atgcattgca ccgagtcggc ttgacaaatc atatgcataa aaagccaaat cagttatctg
    35581 gtggacaaat gcagcgagtg gccatcgctc gagcactagc caatgaccca gatatccttt
    35641 tgtgcgatga accaaccgga gcgttagata cagaaacaag tatccagatc atggaattga
    35701 tccaagagtt atcgaaagaa aaattagtga tcatggtcac acataatcct gaactggccc
    35761 atcaatacgc agatcgaatc attgaatttt ctgatggtaa gatcttaaca gattcccatc
    35821 ctcacatcga acgaccaaaa gatgatcaat ttaacttacg cagaacaaag atgagttttt
    35881 ggacagcgtt aaagctttcc ttcaacaata tccgaacaaa aaaaggacgt actttcttga
    35941 cctcctttgc ttccagtatt gggatcatcg gaatcgcgat cgttctgtcc ctttcttctg
    36001 gattccaaaa acaaatcgat aacacgcaag ctgaaacgat ggcgaaattt ccgattacta
    36061 tttctaaggt aacgactaac cagactcgtg acgatgccgg attgggtgca agcaaagcgg
    36121 attatccaga cagcaaaacg attactgcaa aagtcagcga agaagataaa gcacagcaca
    36181 cgaataagat tgatcaaaca tacgtcgatt acgtaacaga catcgatcct aacttaagta
    36241 ataatatcgg atttacccgt actacaggaa tcaatctttt acgtgatgta aatggcaaag
    36301 tacagcctgt cagcttctcg aatcaaaatc ctgatacgga atctctttct ttatctagta
    36361 cgatgtcagc aatgacaggt gtgggagtgt caagtttccc tacacagctt gacactagca
    36421 aagaaaactt tttaaaagac aattattctt tacttgccgg aagttatcca gcctcagcaa
    36481 cagatgtcgt gctgatcgta gacggcaata ataatactaa tatcaacgca ttgaagaatc
    36541 ttggatttga tgtgaaagaa gacgaaaaac ttgattttga tgatatcgtt ggtacaacat
    36601 tcaaattagt aaacaacaat acttactata caaaacttcc aactgggaat tttatcccta
    36661 atacagatta tgatgcgatg tatcagaatg cttctgatga attgaagatc tcggggatcc
    36721 tacgcgtgaa atcttcttct acaatgaatc tcttatctcc aggaatcgct tatagcgatc
    36781 aattaacgac tcaaatcgtc aacgaaaaca aagaatcaga aatcgtcaaa gctcaaagag
    36841 acagtggtgt aaatgtctta actactgaaa aagttgacga aaatgccaaa caaacattgc
    36901 ttagttattt aggtggagac agcttgcctt caagtatcat gatttatccg aacaattttg
    36961 aagacaaaga aaagatcttg gattatctag atgattacaa taaaggcaaa tcagatgaag
    37021 ataaaatcat ttatactgat ttagctggaa cgatgactga actgactggc ggattgatgg
    37081 atgctatcac ttatgtcctt attgcttttg cagggatctc tttagtaaca agtatgatca
    37141 tgatcagtat cattacttat acatctgtga ttgaacggac aaaagaaatc ggtgtactaa
    37201 aagctttagg tgcacgtaaa aaagacatca ctcgcgtatt tgacgcagaa acgtgtattt
    37261 taggtatttc ttctgggata ttaggtgtgt ttattgcatg gctagctacg ttccctatca
    37321 atagcatctt atacaacatg acggatctaa aaaatgttgc tcaactaaat cctgtccatg
    37381 cgattattct agtgatcgtc tctactatat tgacgatgct tggcggtcat cttcctgcac
    37441 gaatggcagc gaaaaaagac gccgctatcg ctttgcgtgc agaataaggt tgtgacaacc
    37501 acatttagat ttgagtcata agagcgctga aatagtagaa aacagcaaga aacgaggaag
    37561 taatatgttc cttgtttcgc gctgtttttt ttaattattt tttataattc tggtatactt
    37621 ttctttcctt tgtaccacaa ctcttctggc gtatacctgt cttttccatt tatgaatact
    37681 gttccatctg cttcaatcgt tacttcagtc ccaatccaca tattacgaga aagtgcgaga
    37741 agattttttc tttttcctct tccttttttt ccaaattgca aggcatcggt tatctcttct
    37801 gtccaatgag atttcacgta ttttacttct ccgttttcaa atgtaacttt caaaatattc
    37861 gctatatttt ccacttttac tgctttagga aatgactcct ccattttttt cactttcctt
    37921 cctccaacac taagtatgcg ctttctttta gtgataagta tatcatagtt tattctatta
    37981 tcaaaaaaac gcatccattc cattgtagaa tactttaccg aacaagttga attgttttcc
    38041 tctttttcac cttttactat agataggtaa agacaactaa ggaggaatct ctcatgacag
    38101 atcaaccgta tgatttaaga aaagtcatcg aagaactaaa aacacttcca ggtcaatatc
    38161 acgaaacaga tattgaaatc gatcctgaag cagaattatc tggtgtttac cgttatatcg
    38221 gtgctggcgg tacagtaaaa cgtccgactc aagaaggacc agctatgact ttcaacaata
    38281 tcaaaggttt ccccggcaca cgtgtgaaca tcggaacaat ggctagtcgt aaacgtgtag
    38341 gtgaaatttt gcaccatgat tatcgaacac ttggtcgttt cttgaaagac gctgtagaaa
    38401 atccaatcaa acctgtgaaa gtagacaaag cagacgctcc agcacaagaa gtcgttcatc
    38461 tagctaccga tgcagacttc gatattcgca acattttgcc tgcacctaca aatactcctg
    38521 aagatgctgg tccatacatc acaatgggtg tagtcttagg atcagatcca gaaaaaacaa
    38581 tgagcgatgt aacgatccat cgtatggttc tagaagataa agatacgatc ggtatgtaca
    38641 tcatgcctgg cggacgccac attggtcact tccaaaaaca attcgaagcc ttgaataaac
    38701 caatgcctat cactatcaat attggtttag atccagcaat tgccattggc acgactttcg
    38761 aaccacctac cactccactt ggctacaatg aactatggtg tgcaggagct ttgcgtaacg
    38821 aacctattca aatcgttgat ggtatagcag tcgatgaagt gggtattgct cgtgctgaat
    38881 tcatcattga agcagaaatc atgccaaatg aaacaatgca agaagatatc aacacgaact
    38941 caggaaaagc aatgccagaa tttcctggct atactgggga tgctaatcca gctgtacaag
    39001 tagtgaaagt caaagcagtt actcaccgaa aagacaatcc aatcatgcaa acaacgattg
    39061 gtcccagtga agaacacgta tctatggccg gtattcctac agaagcaagt attcttgcct
    39121 tggtcgataa agctattcca ggaaaagtag taaatgtcta caatcctcct gcaggtggtg
    39181 gtaaattgat gaccatcatg caaatccgta aagaaaacga agcggatgaa ggtattcagc
    39241 gtcaagcagc tattcttgct ttatcagcct tcaaagaatt gaagacagtc attttagtcg
    39301 atgaagatgt tgatattttc gacatgaacg atgtagtttg gactttcaac actcgtttcc
    39361 aaggagataa agatatcatc gtgatcccag gaatgaggaa ccatccattg gacccatcag
    39421 aattaccaga atataatcct ggctctattc gtttccgtgg gataagtgca aaaactatct
    39481 ttgacggtac agttcctttc gatatgaaag aacattttgt acgtgcaaaa ttccaagaag
    39541 tagaagattg ggaaaaatat ttgaaataat gaatgatctg caactgtttt gacatcataa
    39601 aaaggagtgc ggaaaatgga acaattggca caacggaaga aaaaaatcgt gattggtgta
    39661 agtggcgctt caggcaccat ttatgccatc aacctagtga agaaactaaa agaatatccg
    39721 atgatagaaa cacacggaat catcagtcca tgggcaaaac agaacttaaa gctggaatcc
    39781 gacttatcac taactgaatt cgaaagcctg tttgattacc attattcaaa caaagattta
    39841 ggtgctgctg tcgccagcgg atcatttttg accgatggaa tgatcatcgt accggcaagt
    39901 atgaaaacag tcgcaggtat ctctgttggt ttaggcgaca acctgattgc acgtgctgct
    39961 gatgtaacaa tgaaggagca acgaaagtta gtcattgttc ctcgtgaaac gccattaaat
    40021 gccattcact tggaaaactt gactaaatta gcacgactag gtgtgcaaat cataccacca
    40081 attcctgctt tttataatca gcctcaaacg atccaagatc ttgttaatca ccatacaatg
    40141 aaacttttgg atcaatttga aattgtaaat gagcattcaa aacgttggca gggtgattaa
    40201 tatggaaaaa gaaacgatag ttgaagtaac caaactaggt ttcacgatcc aagctgctgt
    40261 taaagtaatc ggtaaagact tattgatcac tttaacaggc ggagatacac cacatatcgg
    40321 aacagtcact acattttctc gtgatacaga gatgcaaact gtacgtttcc ctagccacga
    40381 tggtcggttc cataaagacg atgtcttagc cgaaaaaata gctgtgatca tcaaaactgc
    40441 cgttccggga agttgtacga tcacttccgg tatccatgtt gaccatatct ccaatgcaca
    40501 aatcaacgcc tcttttccga tgtcagaaga gttaggttac aagattttag attggttgga
    40561 aaaaatagat ttccatatca aagaaccagt ttattataaa gatggtgaaa aaccattatg
    40621 aaagaagagg ttgtgaaatc agcgttatga cctgagtatg agagaaaaaa atggaaaaat
    40681 agcttctcat attttgccgt ttttgagcta ataccgaggg acagctgatt gaacgccgtt
    40741 tatcaagagg ttgtgataaa gccgcttcgt ttcgagtagt aagaaaaatt ttgtaaacat
    40801 gggtttccac atttttgcat aaatttgact tactaccgag aaactggctt ttgaacaccg
    40861 tttatcaaga ggttgtgaca cgtcttttgt cacaacctct tttatttttg cttcactgta
    40921 actgctacaa tatcaccgat ttcttttccg atgtttttac gaatatcttt tcgtagtcca
    40981 aggatgtagc agactgaacc atctggattt ttcacgccca tattcacgat actgccttca
    41041 tacggcacat tgtcaaaagt tgcttggact ttaactcttc cagcaccgaa ttcttttctt
    41101 atatcatacg gaaaaataat atatgccccg cccttgccga cttcggaagc atagattttt
    41161 gattcaaaag aataaatttc cctcatttta tttctcctat tttttacatc tagtatagca
    41221 tagcaaaaaa aagaaggaac tgtgacaaaa gtcttgtcgc agttcctttt ttcgaataaa
    41281 cggtgttctc ctgccaactc ctcggtcaca agtcacaata gtcaacaaac atgagaagct
    41341 gttttttgaa attgctcctt gtgactcaga gttaaacggc aggctcacaa cctcttcctt
    41401 aaacggtgtt caaaagcttg catctacggc actaagccca atcagatgaa aaatatggag
    41461 tgctattttt atctgattgt tcttactgct tgatgcagag cagctttttc acaacctcct
    41521 acatggtgtt caaaatatta ttcgcctttt gcttctttca ctaattttgc gaatgcttgt
    41581 tgttggaatt cagcagctaa ggctttcgta cgttgttctt gctctgcttt gctcattttt
    41641 ggattttcag tcattgcttt taccataccg tcttgaacat aagcttttgc ttcactttcc
    41701 atcgtacttt gctgtttctt cactaattca gctaacttgg ctgcttggtc cgtacttaat
    41761 tctacccatg tctctgggat tttgaatgta ttcactcggc tgtcatattc gtgattgtta
    41821 tcagcaatat tgaaccagaa tttatacatg tcgtttttgt attcccaata agtagttttc
    41881 gtcacttttt cagcatcgcc gtctgtcttt tctactttat ttgtttcgtt atccgtacca
    41941 gttgcttttg gtttttcttg tttttcattt gttcggtaaa gatacacttt ttcttttcca
    42001 ttccctaaag ctttatacaa aagcatatct gctccttggg aatcagcact ggaaacaatt
    42061 ttttctgttt ttgtttccgt tactttttcc attccaaagt gattggacaa attagcgacg
    42121 atcaaactta gtgaagcgac gaagattagt ccgaaaatca aagataaaaa ggtttgccat
    42181 gtttttttag cgaacacgtt cgttaaagca aaagcaagca agctgacaat caaaactcca
    42241 ataatcatgc attatccccc gctttcatta gaacaggttc ttttttcttc aataaaaagg
    42301 caataataaa gccaagaatc ccaaagatga tcgctacgat aaacgcggca tggtaacctg
    42361 acagtgtggc attgattgcc tgatctttat aagctaatgg cgtattatcc aatacagatt
    42421 tgcttgggat atttgctttt gtcacattcg tcaacacact gatcaaaata gctgtaccaa
    42481 tcgagcttgc gatttgacgg aatgtattat ttaccgctgt cccatgactg atcaagttat
    42541 ttggcaatgc attcatacca gaagtcgtta ccggcatcat gaccatggag atcccaaaca
    42601 tacggatcgc ataaaggatc acgatatata aaactggagt cgtttttgtc aagaaagcaa
    42661 atggtgcagt tgccgctgtc aaaatgaaca tccctgtgat ggctaaacgt ttcgcgccgt
    42721 atttatcgaa aatcgcaccc gtcacaggca tcatagcccc catgatcaat gcacccggca
    42781 acaacatcaa gccagaatgg aatgctgatt ctccacgaat attttgaata tacaatggta
    42841 cgaccatttc tgcccctacc atcgccatat ttgttacacc agctaaaaca gctgcaatcg
    42901 tgaatgtaga tgatttgaat acacgaagtt ccaagaatgg atgttccaaa tgtaattgac
    42961 gccatgagaa caaggcgatg aaaatcacac caacaactaa gaaaccaatt actttcaagc
    43021 ttccccagcc gtcatctcct acactagaaa atccgtaaag taaactaccg aatccaatcg
    43081 ttgataaaat agcggataaa atatcaatgc taggatttga caatggaata acacttttca
    43141 ttaggaagaa tgctaaaacc aagacaagaa caacgattgg cagtaccata ccaaacaagt
    43201 cacgccaatg gtagttatca atgatataac cagataatgt agggcctaaa gcaggagcaa
    43261 gtccaataac gatccctgac atccccattg ctgatcctct tttttctggc gggaaaatag
    43321 atagcatgat attttgcaat aatggcatcg atactcccac accgcaagct tggatcaaac
    43381 gaccgattag taatgtacta aaattaggtg ctgtaaagca agtaatcgtt ccgatcaaaa
    43441 agatcgtcat ggcagaaaga tataaatttc ttgaactgaa tttattgatc agccatgctg
    43501 ttattgggat catgatcccg ttgactagca agaaaccagt cgttaaccat tgcacatcag
    43561 acgctgaaat atcgaaagct ttcatcaatg taggaaaagc tgtcgtcaat aaggtctgat
    43621 tcaaaacggt acaaaatgta ccaattaata agacaacaac taaaagtgtc cgattatatg
    43681 gttttccgta aatatctacg ggttggttgt tactcatatt tctccttctt tctgaatttt
    43741 ttctgaaact actagaatac tttagtccta aattcgctat ttgtcaacct acttgacaca
    43801 taagttaata cggatataat taagggaagc aagaggacaa aaggagtgac aaaaatggtt
    43861 ggggcttccc ttaaacgttt gttggaaaat gatcaattgg ttgttggcat cagtgaatta
    43921 agtgatatcg taggtgtgtc tccacgtcaa ttaaggtatt gggaacaaaa gggattcatt
    43981 cgttcgatcg aagctgatgc gaattgtcct agaaaatatc gtttacctac agtcataaaa
    44041 gtagaactga tcaaaaaata tttagatgac ggatttactt tgtcaaaagc tgcagaaaag
    44101 gcagaaatcc gccaaaagaa aatgcaccat gtccgtaaag ttttttcaaa agcaatcaaa
    44161 gatatcgaat tgatcgacga acgctatacg attttacaaa tcggtaactt caaagaagag
    44221 aatgctaaga tgtacatcat tcatgacaac gagacagacg aattaactta caagctcctt
    44281 cctgtcgatg aaaaagctga ctacgaacag ttgttaaaag aactctaaac gccactaatc
    44341 tcctaagtac caagcttttt aataagtaga tgctacataa atacttgaaa aaaggcctgt
    44401 ttgaacataa gaatatccga acggtgatct gcgtttcttt catatagaaa ggaattctcc
    44461 atcactgttc ggatattttt ttattttttt cggttacttt cttctaatac atctgttagc
    44521 caatttgcat attctgtttc ccgattgatc gcatgcgcta aaatcaggta gtgtccgtaa
    44581 ttttcatgtt tttctttttc agtaggaaag atcgtttttt ttcgctctaa tagatgaagt
    44641 cgtttttctt catgaaggtc acgctgctgc tgaataattt ctactaatgc cggatcattc
    44701 acgtctttga cgaaatacaa tttcagaaca aattcgtctc gattaaccgg tagttcattg
    44761 gtgggtgtat gaatccactc tgccaattgt tccttccctt cttctgttaa ttcataaact
    44821 ttcttttcca atttttcacc tgtgatctct acttgatgct gaatgattcc ttgctcttcc
    44881 atttttgcaa gttcaggata aatctgacta tgttttgctt gccaaaattc tcctatttca
    44941 tttttgaacg attgattcaa ttcatatcct gtcatcttct tcttacttaa taaccctaac
    45001 aaaatatatc gaagtgtatt ttttcttgcc atcagttttc cctccttgac aaatctttaa
    45061 atccattcta tcataaatga gtaatcgaaa acatgtagtt tattacatgt tttaatttat
    45121 aaggagatgc ttaaacatga aagaatttaa agaattggaa gattttattg gaactcactt
    45181 tatctatacg tatgacaatg gttgggaata tgaatggtat gccaaaaatg accatactgt
    45241 tgattaccgt atccatggcg gaatggtcaa aggccgttgg gtaaaagacc aagaagctga
    45301 tatcgttaaa ttgacggacg gcgtattcaa aatcacttgg acagagccaa cagggacaga
    45361 cgtggcattg gactttatgc caaatgaaaa gaaacttcac ggaatgatct ttttccctaa
    45421 atgggttgaa gaacatccag aaatcactgt tactttccaa aacgaacata tcgcagaaat
    45481 ggaagcagct cgtgaaaaat atgaaactta tccaaaatta cttgttcctg aatttgctac
    45541 gatcacttat atgggtgatg caggtataga caatgaagat gttattagcg aagaaccata
    45601 cgatggaatg ccagatgata tccgtagcgg aaaatacttc gatgaaaatt ataaacgagt
    45661 aaaataaggt tgtgaaagag aggttgtgaa atcagcgttc tgacctgagt attagagaaa
    45721 gaaacggaaa aatagttcct catgttttgc cgttttttga gctaataccg agggtcagct
    45781 gattgaacac cgtttatcaa gaggttgtga aatcagcgtt atgctttgag taataagaac
    45841 gctagttaga aaatagcttt tcatattttc taggctagtg ggcttattac cgagaagcag
    45901 ccgtttgaac accgtttatc aagaggttgt gaaatcagcg ttttgacctg agtattagag
    45961 aaacaaatgg aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca
    46021 gctgattgaa caccgtttat caaagattgt aacaactgca tattcacaca gttaaattag
    46081 tgacttcaaa atgaaataat gctcccagca aaaaattagg ctgggagcat tatttcgtta
    46141 ttctgttcat caaaaatcag cgttttgatt ccatttcatc acaagttcct cctcatttac
    46201 tgcttcatct ttttgctgtg tttgaattgt aaaaccttgt gaaagataga aatgaatagc
    46261 tgattgattt ttttgataga ctgtcaaagt cagtgtttta ttggtttctt ttattttttt
    46321 cattagttgc gtaccgattc tttgttgccg gtattccctt ttcacaaaga ttcctgcgat
    46381 aaactgatca atcaccccag caaaaccaat aatttcttga tctatttctg ctaagtaaat
    46441 cactgcatct ggtaaagctt tcctaacaga ttctacttga ttctcccaat aagttgatgg
    46501 aatgaattca tgagaatcca agttagcatg cagccaaatc gatagaacag attccagttc
    46561 tttggcagac aattttgttg ttgtgcggat cattttttct ctccttactt attcattgat
    46621 tttaaaataa aaaccatttt tttgaagctc ttttacaagt tttcgcccct caaaaaagta
    46681 tatcttctct tgttttactt ttctaatacc aaaagaaaca gcaactttag gatcatcagc
    46741 tgaaacgatt cgataagttc caccaggcaa ttgatccagc ttgactttcc catgatggtc
    46801 gctttgtact gaaaacggca caccatttct agtaagtgtt tttttcttat gtttcaaaat
    46861 gaatgaacta ccatttaccg gtttccctga cgaatcacta ataataaatg aaaaatcaag
    46921 cactttttta cttaatctat actgatagat tagtcgtccg ataaacacta acagtatgac
    46981 tacagctatt atcgttcctg caagaataaa ccattgccgc atggttcttt cctgatttcc
    47041 ttttccttct tcttttttta ccgattcgtt ataaggaatc cgatgtcctg tcactagcaa
    47101 acgatgcgtg ttgatcatat acggcgtaca tgtaagcaag gtaactaagt cttctccttc
    47161 tgaaatcgtt agaacagacg tgtcatctgg cagtaccact tgtatatttt taacttgata
    47221 agccaatttc tcccccaaaa cagtcaaaat aaacgtgtct ccgttttcta aacgatctaa
    47281 ttcggtaaat aaccgtcgat taggtaaacc gctgtgagca gagatcactg aatgcgtcga
    47341 atttccacca agcgggaaag aggttccttg caagacgcct gctccatttt caaggatcct
    47401 accattgagc cgatcataaa gtgaaatatt taattggatc ttaggtatat tgatacttcc
    47461 gatcagccat tcctctaatt ggctggatgt tgtactttca tttctgttct tttcattgaa
    47521 aggatcagta ccaggattga atccttggtc agttaatcgt tggtttttct cctccatttt
    47581 ctttcgctgc acttcaattt cagctgcggt ccgtttttgt acttgttcga tacgtttttg
    47641 atcaataaaa ctatttaaag aatccacata aaatggatat aaagcgatca gtaaccctac
    47701 gacgatacat attaataaaa acaaacgaaa caaaagtcga cgagactgtt tttgcttact
    47761 ctgcttcaca tgtgtcctcc cttgcaagtt tttttgttct ttcttttatt atatgggttc
    47821 aggaaaaaaa tagaaaatga aagccgatga ggttgtgaaa aagctgtcct gcatcaagta
    47881 ataagtacag cccaaaaaaa tagctactca tattttttgt tgggttgggc ttattaccgc
    47941 agatgcaagc ttttgaacac cgtttaagga agaggttgtg aaaactgcgt tatgctttga
    48001 gtaataagag cgctagtcag aaaatagctt ctcatatttt ctaggctagt gagcttatta
    48061 ccgagaagca gctgattgaa caccgtttac cgagaggtca tgaaaaagct actttgtttc
    48121 gagtattaag aaaaattctg tttcaaaaca aggagatgca acaaaactta tgtcgcatcc
    48181 tctctcctta gaactttcac ttatgttttt cttgtaaaat tcataaatat tttcatgaat
    48241 tttctaattt cttgcctacc tctcgcatag gatatatgat gaatttatct aaagtttgca
    48301 aaggagttcg acaattgaaa aaatatggcc catttttagc aatttttttc attgctttgg
    48361 catttctcat gcctcattta ttcacacata aactaatttt gggtgctgat gccccattcc
    48421 attacaatcg gttctatgat gccgcgcaac aaattaaata tggggatttc cattattttc
    48481 cttcccttta tggattccag caaagtggca ggatcgtgaa tgccgtttac ggcccatttt
    48541 tcgcttattt acaaggattg atcgtgttgc ttgcaggtag ctggtttcat tatcagattc
    48601 tttcaaattt cattctttat cttatttcag gattttctat gctgatttta ctgagatatg
    48661 tcaatgttcg aaattgggta agtgtaccag tagccgtttt attcatgagt acgtattcta
    48721 ttcaatactg gtctatcaac caaggattca ctagctgggg gacttgtttt tatcctttat
    48781 gtatgatccc tattgctgat ttcgtccttc ataaaaagtt ccctgttatc aaagtcgctg
    48841 tctcagtggg acttatgaca cagatccatt tactgtcttc gatcatgtta gctttgatgt
    48901 atatcccttt ttatctttat tactttttta accagactga aaaaggaaaa gcactgcttc
    48961 aactgattaa gtcggtcttg ctttattttg ttctgacagc aaatatttgg gcaggattag
    49021 ctattgttta cagtgggaat aagattcttc ctccctttgt taacagagaa atgagtcaaa
    49081 aggcgataga tctggatggc agttattggc tccattaccc taaggccttt cctatattga
    49141 tattatcagg ttttttgctt tttattttct ttcaacaaaa gaacacctta ttccaaaaaa
    49201 tcgtgttgta cacaagtctt gttttctttt ttctttctac tagcttgatt ccatggacga
    49261 cattgatcga aaaaaatgtg ccttttatta gtttgatcca gtttccattt cgatttttcg
    49321 tgccatttac gctattgctt ctactttacc ttgccctttc actgaaccag tggtctgaac
    49381 aaaagtggtt ccgtctttta agtatatttg gcattcttat tttttcagca cagacgatcc
    49441 agaacttata ccagcatctg gaaaagtggg aaaatgaaac atttgtcagt cgacatactt
    49501 atctattcga tacgccggaa gaagcaagga aaacattctt cagcaaagat atgtctacca
    49561 gcttgttcgt tttccaaaaa accacacctg actatctgcc aatctataag gagacaaaag
    49621 acaataaata cgaccggtat aacgaagaaa tactagaaaa cgaaacaact tttataaaaa
    49681 cacatccagg cggtcttttg acaatcaagt ggaaaaacac tggggacaaa actgttcata
    49741 tccctgttat cgtctacgat cggactgtgc tacagcaaaa tggaaaaaca ttgactgatt
    49801 atgaagtaac agatatcggg accccaatcg tgaagcagca aaaaggaatc aacgagctca
    49861 cgcttcatta tcaaacgcca atctatttct atttcattct ttctcttact ttgattggct
    49921 ggtttacctt gctatgttta tttatttatc atagatacaa gcttttacgt gcttaggcaa
    49981 aagggataaa tacatccgat caaaaaactc tccaaacggt ataatcgttt ggagagtttt
    50041 attttaatca gtttttttat gttatatgtt atatttttta ttcgttcaca tctaattcga
    50101 tttccacccc gtagtgatca ctgatgattg gactatcgat cccatcaaaa atccgttgat
    50161 attttttaac ctgcattcct tcagggacga aaataaaatc tatacgcaat ttttctgtat
    50221 tttgttccca accatctatt ttcttttcca cagtagctgc ttttcctttt tcattcgcga
    50281 caacatacga atcttgaaga agaaggcaac tttctcttac agtataataa ccttgagagt
    50341 ctactggatt attgaaatca cctaggaaaa aaagtggtac gcgagtttcc aaaaagtagt
    50401 tttccaagtt tttccactca aaataaaagc cacttttttt atctgtccac caagaaaagt
    50461 ggcagctcga gacaaaaatg tcctgatcat ctatctttgt tttcccaatc aagatcttgc
    50521 gtgtctgata atcttctttc ttttgaaatt gggagacggt aaggacctga ctttccaatg
    50581 tttttttcgt aagcagtgca tttccttctt catatttatc ataaccgatg tggctcattt
    50641 cccaactcca aaaatactct tttccgtgtt cctttaaaag ctgaactaaa cggtaggcaa
    50701 aattatcttt acggataggt acttgattct ttaccggaca gaacttcatt agcttcgcag
    50761 gttctacttc ttcagattcc agtaattggt tgatttcttg taaagcaatc acttcataat
    50821 cttctaacag gatcttatct gctaattctt gcaatttttt ctctggttct ggctcaagcc
    50881 agctatgcgt gttgagtgtc atgaatttca ttggttttct ccttgtcttc atttcgaaac
    50941 aaaagggttg tgacaaaatt tttgtcacaa cctctccagc aaatagtgtt ctcctgctaa
    51001 ctcctcagta ataagctcaa tggaccagga gctatttttt gtttggctgt ttttatcact
    51061 tggtgcaggg cagctttttc acgacctctc cactaaacag tattcaaact gctgcttctc
    51121 ggtaataagc ccaaccaaac gaaaaatagg aggagctatt tttgtttggc tgttcttatg
    51181 acttgatgca ggacagcttt ttcacaacct ctccttacat tttaatggag ccgatcactt
    51241 taccagctgt cactgtacct aattgcttga tcactacttc ttctacttta tcgtttgtaa
    51301 atacgatcat gatcgttgga tctttttctt cgttttggat cacatcaaga tccatctcag
    51361 ctaatttcgt tcctgttcct acttcttggc cttctttgac agcaatctca aaagctggct
    51421 gattcatttg gactgtatcg attcccatat ggatcaaaac ttcgatacca tctttcgttt
    51481 ccagcccaat tgcatgtttg ctagggaaaa tacttgtcac ttttccagcg atcggcgcaa
    51541 caacttctct tgatgttgga ataacagcaa agccgtcacc catcattttt tgtgagaata
    51601 ccggatcttt tacttcagat aaaggaatca cttctccaga agctgcggaa actagttctt
    51661 gttctatacc taagtattgc ttactacctg cttgtccatt tcctgtagaa accatttctg
    51721 gctctggaat ctctgctcct gattgcagca gatcttcgat atctgatttc aagacatctg
    51781 cttttggtcc ataaactgct tggactccat tgtctttgac gatcaatccc atcgcccctg
    51841 ctcgtttcca ggcttcttca gtccctactt tttctcgatc atttacactt acgcgtaacc
    51901 gagtcataca agcatcgaca tctttgatat tttcttttcc tcccagcaaa tgaatgatct
    51961 ggaccacttg ctgatctgct gttcctgttc cgctgttaga tacaacggtt tcttcactgt
    52021 cattgtcgta atttccgtta cgtcccggtg tagcaaagtt gaattttttg atcaagaaat
    52081 ttgctaagaa atacgtaaca acaccaaaag cgatcacaca caacacgaag ttgataagat
    52141 ctccacctag acccgctttg atagctaacg gtgtccgagt cagcaattcg atattcccaa
    52201 atgaatggac ccgcaatggc aagatatctg ccatcgcaaa tgcagcccct tggatcactg
    52261 aataaatgac atataatggc acagcagcaa acataaacat gaactccaat ggttctgtta
    52321 cacctgttag aaagacagcc aacgctgctg aaaaatacat tgatttatat cttgctttct
    52381 tatcaggatc gacattacga tacatcgcaa aagccattcc catcaagata cctgaagaac
    52441 caatcatttg tcctactttg aaacgagccg gcgtccagtt atgcaacaca aactcatatt
    52501 gcgacatatc ccctgcacct ttaagattca ctaaatcagt ggcccaagcc aaccataacg
    52561 gatcttgccc aaatacttgt gtccccgcct gtgcgcctga taaaatctca tacgatccgc
    52621 caagctgtgt atagttgata ggaatcgtca gcatatgatg caatccaaat ggcaatagta
    52681 aacgttctaa tgttccaaat aaaaacggtg ctagaatcgg tgcgctttct tgtgattctg
    52741 caatccataa accaaaatta ttgattccag cctgtacgtt tggccacacc acagcaagaa
    52801 tcaatgccac gattgttgac cataagataa caacaaacgg aacaaagcgc tttccattga
    52861 aaaatgacaa agcatccggc aattttctat aattatagta cttattataa gccattgccc
    52921 cgacaaatcc agcgatgatc cctacgaata ctcccatatt caaagctggt gcttccaata
    52981 cacttgtaaa aaatccttta accatgatct tcgtaccgaa cagagtgtgg gtaaatgcat
    53041 cttcattcgc aagcatgtca cttgtgacac caaagatagc cccagtgatc cgattgatta
    53101 aaataaatga gatcccagct gcaaatgctc cgcctgctct ttcttttgcc caacttcctc
    53161 ctattgctaa agcaaataaa atatgaagat taccaataat tgcccaacca atattttcaa
    53221 ttactccgcc cgttgtgaca agcggcgtcc aatcaggatt gatcaaggga attgatttcc
    53281 ctatactgat catcaaccca gctgccggca taactgctac cacaaccatt aaagcctttc
    53341 caaatttttg ccaaaactca aaactaaata tctttttcat tccgtttagc tcctttacgc
    53401 aatcggtttc gttaaacgta ttatagcgtg cgttttcttt tttgtaaatg ctttattttt
    53461 tatttttctg tctataccat tctataaaac ctataatacg tgtgttccct tctataattt
    53521 tctcctaaaa atttattttt ttaagctttt ttctatgaaa tattttcttt tgtctattga
    53581 cttgtgcaat cggttgcgtt tattatgaac gtataaaaca gctcattagg aggagaaaaa
    53641 tgaaacagat caaacgacta tttgctattg atccatggaa aatcagcacg acacaattag
    53701 ataaaaaaaa tcttcgactg caagaatctt taacaagtat tggaaacggt tatatgggga
    53761 tgcgcgggaa ctttgaagaa acttactcag gggaccacca tcaaggtact tatttggcgg
    53821 gtgtctggta tcccgataag acacgtgtcg gctggtggaa aaatggttat ccagaatact
    53881 ttggaaaagt gatcaacgcc ttgaacttta tcgcgataga tatttcgatc aacgatcagc
    53941 tgatcgactt ggcttctttg gaaccagaag acttttactg ggaattagat atgaaaaatg
    54001 gtgtattatc tcgtacattc acaatcacta cttctactaa taaagtacgc atttccttcg
    54061 aacgttttct gagcattgtc aaaaaagaat cagcttatat ctatttaact atagaaatgc
    54121 ttgaaggtag cggaacagtc aaactcgtat caaaattgga tggaaatgtt caaaacgaag
    54181 acagcaacta tgatgaacat ttttggaatg agattgatcg tggaactata gaagaaatcg
    54241 gctatttgac gactaaaact attccaaaca actttgggat tgaacaattt actgttactg
    54301 cagcaatgcg ccatttcgtt aatggaaaat cagtcatccc tgactatcac gaagatactt
    54361 tagcattaca tggttgcttg tctgcagaat tatctcaagg tgagacatgg tcagtggaaa
    54421 aagaaattat cgttcttact agtagagata ttccagaagc gcagcaagca gaaaaagctg
    54481 tctcagaatt aacgaaccag acaaacaatt atatccaagc aaaagaagag caaacagcgg
    54541 cttgggcaga acgatggaag cttgccgatg tggtcatcga aggagacgaa gaagcacaac
    54601 aaggtattcg attcaatttg ttccaactat tctctactta ctacggcgaa gatgaacgat
    54661 tgaatatcgg ccccaaagga ttcacaggag aaaaatacgg cggagccact tactgggata
    54721 cagaagcgta tgctgtccct ctttatcttg ctttagccaa acctgaagta actaagaatc
    54781 tgctgaaata tcggcataat caactgccgc aagcaatcca caacgctcag caacaaggat
    54841 taaaaggcgc gctttatcct atggtcacct tcactggtgt cgaatgccat aacgagtggg
    54901 aaatcacatt cgaggagatt caccgaaatg gcgcgattgc ctatgcaatc tacaattata
    54961 cgaattacac cggcgatacg gaatatttga aaaatgaagg actggaagtt ttagcagaaa
    55021 tcgcacgctt ctgggcagac cgtgtccatt tttcaaaacg agcaaataaa tacatgatcc
    55081 acggtgtgac cggcccaaac gaatatgaga acaatatcaa taacaattgg tatacaaata
    55141 cgatcgcagc ttgggtcttg cgttacacgc gagaaaatta tttaacttat caaaaagaag
    55201 ctgctatcac tatcagcgaa gaagaactag ccaaatggaa agatatcgca gagaacatgt
    55261 acttcccagt tgacgaagaa ttaggaatct ttgtccagca tgacacattc ttagataaag
    55321 atctcatgcc tgtatccgat attcccacaa gcgatcttcc tctgaatcag cattggtctt
    55381 gggacaaaat ccttagatct tgcttcatca agcaggcaga tgtactacag gggatttatt
    55441 tcttcaatga tacttttaca aaagaagaaa agcaaaaaaa ctttgatttc tatgaaccaa
    55501 tgactgtcca tgagtcttcc ctttcgccaa gtatccattc gattttagca gcagaattag
    55561 gaatggaaag caaagctgta gaaatgtacc agcgaacagc tcgcttggat ttggataatt
    55621 acaataacga cacagaagac ggcttgcaca tcacttccat gactggtagc tggttgtcga
    55681 ttgtccaagg atttgctcaa atgaaaactg cccatgatca gttgagattc gcaccatttc
    55741 tgccaaaaac ttggaaagct tattctttcc atatcaatta tcgcggacgt ttattgttca
    55801 tctcagtcac agatgaacaa gttactttcg agttattaga aggttctcct ctagaaattt
    55861 acgtctatga acagtcttat tctttagcgg atcgtctaga aataacgcta agaaaggagg
    55921 aagcaaatgt ttaaaggggt attgtttgat ttagacggtg tcattaccga tactgctgaa
    55981 ttccattatc gtgcttggcg aaagcttggt caggaaatcg gcattacgat cgaccacaca
    56041 ttcaacgaac agctaaaagg cgtcagccga gaagactcac tctctttgct tttagcttat
    56101 ggcggaaaag aacactcttt ttcaaaggaa gaatttgcag aattagcgaa aagaaaaaac
    56161 gattattatt tagagatgat ccagacgatc gaaccaaaag atgtcttccc cggggtcgtg
    56221 cctttactgg attctttaaa agaagaaaaa atcaagattg ctcttgcttc tgcaagtaaa
    56281 aacgggccat tcctacttga aaaaatgggt cttacacctt actttgatgc tattgccgac
    56341 ccggcagaag caacaaacgg aaaacctgcg ccagatatct tcctccttgc agcaaaagct
    56401 gtcggactaa aagcagaaga atgtatcggg atcgaagacg cgcaagccgg tatccaagcc
    56461 atcctctcaa gtggcgctca accaattggc gtgggacgtg ctgaagacct tggagaggag
    56521 atcccgcttg tccctgatac gacttttctt acgatcgatt acttgaaaga aaagtggcat
    56581 gaccatggat aatccagaga tctccgtcgt accagttcca gagactgaaa taaagaaaat
    56641 cacattgaag aacgactact tagaagtcgt tcttctcaat tacggtgccc gcctgcacca
    56701 aatcttcgca ccagacaaag aaggaaaatc agaaaatgtt ttattgtctt acgatcgttt
    56761 cgaagatgtt ctaacagaca aaagcttttt tggagcaact gtcggacctg tagccggaag
    56821 gatccgggat gcttcttggg gtgagcatag attggagaaa aactgcggat cacaccatat
    56881 ccacggtggc acgaaaggct ggtctttcca attttgggaa gtagaagttt tcaaaactcc
    56941 atcatcaata ggtgtggtct tttacttaaa agatgaattt tctacctatc caggaccaat
    57001 cactgctacg atcacttacc gtttaacaaa aaacgaatta gagatgacaa cctctgctag
    57061 tagccttgtt gaaaccatct gcaatccgac caaccatgcc tacttcaacc tttcaggaaa
    57121 tggaacaaga gacatctatg aacatcagtt aacggtcttt ttagatggtg tgcttgaatt
    57181 agatcaagaa aaactgccta ctggaaattg gacaaaaaaa gaaaaactgc caatcgactt
    57241 tagaaaatcc ccaacactgc aagagatcct tgcatgttat ccagatggac tagatgatgt
    57301 atttcttttg catcatccta ggctttccaa aacttcctta caattattcg agaagcattc
    57361 tggtcgccaa atgactatag caacttctaa taaaagtatg gtattatttt ctacgactgg
    57421 attcgaagct gatttctctg tcaatggaaa acagatgcat tcaaactacg gattagcaat
    57481 cgaaccgcaa gaaatccctg atatcgttca tttccccaaa tttggatcga tcaacctcca
    57541 tcctggtcaa gaacggatca gtcagacgat ttatcggttc tctgcacaat aacaagagaa
    57601 aggtgttttt atcatggcta ttaccgtaaa agatgtggcg aaaaaagccg gtgttgccac
    57661 ctcgaccgtc tctcgtgtca tcaacgatca tcctagtatt tccgagacaa caaagaagaa
    57721 agtccgaaaa attatggatg aactaggtta tgttcctaac atgactgccc gcaatctagg
    57781 aaaacgcatt tccagtgcca tcggcgtaat cttaccccct cttgattcaa aagagcggat
    57841 cggtaaccca ttttatcttg agatcatgga agcaatcaac gaagaagcgc ggaattatga
    57901 tatgactaca gcaatcgcta ctgctaaaaa ttttgaattg ctgttagaga acgtccagcg
    57961 tatgcattta caaaaacaag tagatggttt tattctagtg tattcagata gtcaagatac
    58021 tgtcattgat tatttatacc aaaaccacat ccctttcact ttgatcggac agccttatca
    58081 tcatgagaac gaaatcgttt atgtagataa tgataaccag cttctaggta aacaagcaac
    58141 agaatttttg atccagaatg gacataagaa tatcctgttt gtgacaaaca cgactcatga
    58201 aagtttgtat ttcgaacgat atttcggtta tcaaaaagca atgatgatgg ctggtcttcc
    58261 tgcgttatcc agtgtagtac tagagcatcc ggaggattat ctgtcctttg agaaaacgtt
    58321 gaaagaaaac gaagccacag cattggtggt cattgacgat atcttcgccc ttcgcacgat
    58381 gcagttggcg caaatgtatg gctatgtcat tccagagaca ctctccgtca ttagcttcaa
    58441 caactcgatc ttctcaacat taacacaccc ttaccttaca agtatcgata tcgatatctc
    58501 cgaattagga cgtgtcgcta tgcaaaagct gcgcgaattg atccatgaag aattgagcag
    58561 tggcgtacgt ttggtcatcc ctcatcgctt gatcaaaaga gagacagtcg ttcctttaga
    58621 aaacccatac taaatagctg actcactaaa tacgctgacc atttatccta tggtcagctt
    58681 tttatgtaaa agaagatact acttctaaca cattgtttat ctgtaaaaaa gagcgtggga
    58741 caaaagtaaa gaacactttt gtctcacgct ctaaacacgt ataaacggcg gaagcagaag
    58801 caactccttc ggaaataagc tgaaattcac aaaaatttaa aaagcaattt tcttgagttt
    58861 cttcttattt cttggagtta aacgcttctg tcccagcctc ttttttcctt ccttcaattt
    58921 tttacacgtc gacgattcaa taaaaaaccg cctaatattg taaagacagg tgcaagaatc
    58981 gagaaaaagg cgaatggcaa gtaatgcaac gcgctaactt tcatcgttcc cataatgaat
    59041 gtgccgctga ctccccacgg tacgagagaa ttgacggtcg cacctgaatc cgctagtgtt
    59101 cgtgtcaaat aggtttttga taagccaacg tcatcaaaag aacgcttgaa tgtttctcct
    59161 ggaagaatga tggacagata ctgttctcca acgatcagat tgattccgat acagctcaag
    59221 gcagtaaacc cgactaaacg agaaggtcgg tccatctttt cttttaattc ttgaacgatg
    59281 gtatcaacga tcagatattt gatcagtaac ccacctaatg ccaatgcaag taaaatcaaa
    59341 ctagcagaac ctaacatact catgatcccg cctcgagaaa gcagttcatc tagctttgga
    59401 tcaggtgtat ttgcgacata accattcatc aagatgtctg ctgcttttcc gaccgtcaaa
    59461 cttttctcat ggataaaaga aagaacgaga gcagctgtcg atcccgctag taatgtaggg
    59521 atagccggaa tttttttgat tgccccccca aataaaataa ctagtggaat caatgtccaa
    59581 ggagagatcc aaaagctcgc tttcatgttt tggatcattt cattgatact agctagatcg
    59641 gctgaatgac tgttatgtcc taatatcgta taaaaaatca acgaaaggac gaatgctggg
    59701 aaatccgtat aacgcatacc agcgatatgt tcgaatagat taactcctcc aatacccgct
    59761 gccaagttgg tcgtatctga taaaggcgag cagttattcc ctaaaaaggc accggaaacg
    59821 actgcgcctg tagtcattgc atcattgaat ccaagtaaat gaccgatccc caaaaaagca
    59881 attccaatcg tcgaaatcgt tgtaaatgaa ctgccgacag tcactccgac aattgcacaa
    59941 acaagaaatg ctgtcggtaa aaagaaacgc acagaaacaa gtttaaatcc ataaaccata
    60001 atcgtaggga tggttccaga taaaatccat gaagcaacta agacaccaat caaaagaaaa
    60061 atcaggattg gaatgatccc tggagtgatt cctttgatga tcccgtcatg gatctcatcc
    60121 catgtgaact ttttaaatct gccataaaac aacaaacaca tgaaagctgc aaggatcggg
    60181 atatgtggcg agagctggaa tccgatgatc agtactccta ggagtgccaa caacacgatc
    60241 aatacagcga tactttcttt taaagaaata tttttattca tcgtcgatct cctatttcaa
    60301 atgaaaaggc cctgccatga aaaaactatt cacttggcag gacctcctac taaatcagcg
    60361 acaaaacgtc ggctgaaaga cttatttttt tatttttctt ctaaactttt ttctacctca
    60421 atcccccaag aatctttctt gaagtgattg atgatcaatg gaataacgaa catgaccact
    60481 gtgatagctg ctactaataa tgcatcactc aatgtcgctg tacctaatgt agcaaatacg
    60541 acaacgatga tagagaagat cagcatgcag ctgactaccc aagctaatcc gtttccgttt
    60601 ttaccaattc ggtaaggacg ttcgaattct ggatgtttct tacgcatttt aagcaatgaa
    60661 acagcaatca aaatgtacac gatacaataa ataacagttg tagcatttgt aagtgtcaag
    60721 aatacagcac ttacgtcatc cattacacca taaagcaaag caaataatga aataacgatt
    60781 gattgtgtta gaacgacatt tctagatacg ccgtatttgt tttctttatg gaatccgaat
    60841 tttggtggaa ggaatccttc acgagctact tggatgattg tttttgatgg tccagtaacc
    60901 caagctgata attgtaaaag cacaccaata aataccatga agctgaagat atttccgatc
    60961 acttctggta agcctaggat ttgacagtca atcaagatag gctgcgtgat atttgctaat
    61021 tccatttttc catctggtac gacatttgct acaagcatcg cattgatgac attcaataaa
    61081 actaatccga tcaaagcgat aaatacgcct tttgtataat tttttgttgc gtctttcaaa
    61141 cgaggcatgt aaacagaact catttcgatc ccaacaaaaa taaatgtgat accagctaag
    61201 tatttcaatg aatcaatatg ttccaagtca gggaaagctt ttgaccatga aaagtcacct
    61261 agatagccgt ttgagactag tccgactttg aaggctgcca aaacacctaa aacgaacatg
    61321 atgactactg ggatatatac ccctagccat acaccgatgt tcccgccgac ttttgccata
    61381 tcgaatttca agttcaagat cgtgatgatc cagtagataa ctaaaataca acctaaaacg
    61441 aaccagtgat tattgccaag ttctacatta ccaaatgtgt ttcctaacaa tggtcctaaa
    61501 gtagaagcga ccattaccat acctgggaac atttgcaccc ataatagcca tgcaacgaca
    61561 aatccccatt tgctgcccag tgctgttttt acccataact gaggaccgcc ttcttgagga
    61621 agcatcgtgg aaagctcacc ggaaaccatt gatatcggac cggcaaaaaa caaaactgca
    61681 aaaattgaat aaaaaatcaa tgtccaaccg actgcggcta acgtaggaat actccgaacc
    61741 gttgcacaca gtgccattgt cagaccgata aaggtcccaa gacccatttt acttacttca
    61801 gatttactca ccgttgacac tctccttgat tttttggata aaataacggt gtctttgaag
    61861 tagtttcagc aataaacgaa acaattcagc gaatatttga ggggattctt tgagtttatt
    61921 gcttgaaggg tttcttcaaa gacaacctca aaacaaaggg atcgaagagc ttaggtctcc
    61981 ccttgctctt cagatccaaa catgcgtcag aaacagttag tattcaagct gttttctaac
    62041 atgattacct tgcttatttt gcttcgcttg ccaataattg gtctgcgtag attttttcca
    62101 atttttcttg aagagctgct ttgatttttt cagcatattc ttcaaagtgt tcttctttgt
    62161 tcatgtatgg tgtcattacg ctggcacgca atacagttac ttttccggca cggttccatt
    62221 cttcatctga gaatcctagt tggttcacaa attgtaatgg gctgttgcca tagtctggga
    62281 tagcaaagtc agtatgtgaa gttaagaact cattaccgta aatgcttcct ttcacgtatg
    62341 aagaataatc atatacgtca tggttcaatt tattcattgc aactaagtca tcgttgcctt
    62401 tttctttgaa tacatagtct accatattga aatctggata agttaatgga tgaacttcga
    62461 tttctttgtc tcctacttta aatgacaagt cattcaagaa gttgtagaaa cgatgtgcac
    62521 cttcgattga tgcacccatt aatttaccat atcctgtgac attcaaaggt aagacatggt
    62581 gtgctgccca aacgctggct gctgttgccc ctgcttttga accttccaag atataagccc
    62641 caagtaatgc tgggatatct gcgccttttt caaatacata tgtcgcaaag taagagataa
    62701 cgtctctcat gcggatatct ttgatgacga taccaccggc agaatatgga acatagccca
    62761 ttttatgtgg gtcaatcgtt acagattctg cttcttcgat tgctttatat gcactgtgta
    62821 cttcttctaa gatataatct ttgttttctg taaagacatt gtatttgtaa tgaacatctt
    62881 ttaaatcttc aaatggaatg aagttgttgt cttcgtctaa gaaaatcgca cggccgtatc
    62941 cgccataagc agcatctacg tgtaagtaga agtaaatacc gtctttttct aatacgcgac
    63001 gcaatgcaac gattttatcg ataccgtcga ttgctccttc ttctgttgat cctacaacac
    63061 cgactacacc taggattggt gttttttcag ctgctagacc acgaacgatt ttttcaagtt
    63121 cgttgatgtc catgcgatag ttgtggtcaa ctggcactgg aatcacttgg tctaaaccga
    63181 taccgatgat gtcggcagct tttagccatg aatagtgttt agtttgtgga accaaccatt
    63241 ttcctaattt ttctaaatgt tttccgctac gtgctgagtg agctttgatt tcgtcgattt
    63301 tttctgggac gctgtctaac agattcatga tttcttctgt tgacaagttc attaattccc
    63361 aatcgctttt tccagcaact agttcaggtg tcacttcttt cattgcaagc ggcaatgatt
    63421 tgatgttgcg ggcataccaa agtccttcta agtttgctaa agaaccatca gcaacgatgt
    63481 ggccccagcc atctttatag ctcattaatt ttgcaaattc cattcctact tcttcttcca
    63541 tttggcttgt tgcaggagaa gattcatagg caacgttatt accattccat agcatagcaa
    63601 aattgtacgc tagtaaagat ggcatcaatg tttcagagtt catatgaccc cagtaacgtc
    63661 cggcattatg ccaaggaacg gaatgggtac gcatccgcgc tgaaatttct gatagaacat
    63721 cctttgtttt attcacagtg tgttcaaaac tagcactgct tttttcttct ggcgtaataa
    63781 taggcatgtc ttgaggcatg tagttttgac gccagcctaa atgttcatcc accaattcat
    63841 tcaacaaagc tttatagatt tggccgttct cagctttgtc cccaatgaat agggcattta
    63901 ggtttagatc tttcgacaat gattcactca tagtgaaacc ctcctaaaaa aatatttaaa
    63961 ttttgtcgag ccctacatta cacctgttct tctattctgt aaactaagtt ttggcctgat
    64021 ttttaatcat attttaatat gtatcaatta tccgaattgt tcacactttt gaaataatta
    64081 aaaaaaacga gggaatttac aaatgtaaac tccctcgcaa agattgtatt aacgacaacc
    64141 agcgttctat tttaggatgg gtttcaaaat tggaatgttg taatagtatt tccctttttt
    64201 aaggtaaaaa cttactaaat tctaatcaat tactttggcc aaaaagtaat ttttcttacc
    64261 ttttcgaata accacaaact taccatcgaa ttcatcggat ggatttatga caaaatttaa
    64321 atcggtaacg cggtcaccgt taatacttat agctccgttt gaaacatctt cacgtgcttg
    64381 acgtttagat ggttcaattt ttgtggaaac aagcagttcc acgatatttt caggtgtgct
    64441 tgaaatttca acgttcggca ttttaccgaa tccttgcgcg atttcttccg catttagttc
    64501 tttaatattt ccagagaaca atgcttgcgt tattttttgc gcttctttca agtcttcttc
    64561 actatgcaca aatcttgtaa cttcttctgc caaacggcgt tgtgcttcac gtttctcagg
    64621 ttctgtctct acttttttcg ctaagtcttc gatttcttct tgagacaaga aagtaaagaa
    64681 tttcaagtat ttcactacat cgcgatcatc ttgattcaac cagaattggt agaactcaaa
    64741 tggtgaagtt ttcttcggat caagccaaac ggcaccgcct gcagtttttc cgaatttcgt
    64801 tccgtcagcc ttcagcatca atgggatcgt taaaccgaac gcttctgctt ctggtccttc
    64861 taattttcgg attaggtcta atccagctgt gatatttccc cattggtctg ctccaccgat
    64921 ttgcaattgc acatcgtatg ttttatgcag atgcaaaaag tcgatagatt gcaggatttg
    64981 gtaagtaaac tctgtaaatg agattcctac ttccaaacgg ctggcaacga tatcttttgc
    65041 taacatcgtg ttgatgttga agtttttacc gtagtctctc aaaaattcaa gtaaagagat
    65101 ttttgacaac cagtcgtagt tgttcacaaa tgtgatattt gcatctttac caaacaattt
    65161 tctcatttga ttggaaagtg cgtccacgtt atgttgcact tgttccatcg tttgcaacac
    65221 acgttctgtt ttgcggccgc ttgggtcgcc gatcgttcct gttccgccgc caatcagaat
    65281 atatggatga tggcctgcca attggaatct tttcatcatc ataaatggaa tcaaatgtcc
    65341 gatatgcatc gaatcaccag ttggatcaac gccgcaataa agtgaaatgc tcttttcttc
    65401 aacgagctcg cgaagtcttt cttcgtttgt ttgctgatta atggcgtcgc gccaagttaa
    65461 ttcatcaata atattcattt tactgacctc cgtgtagttt ttttatgaaa gcaggaacct
    65521 gctagcatat gtaattaatt atttcttcag gataggatag gaaataaaaa aagtcccttg
    65581 gcatataagc caagggacga aatctcgcgg taccacccaa tttgtatgtc tagatatcca
    65641 acatacctct cgaacattta acgctgtcgc gtggcaatca ctgccaaaga ctcctgaaat
    65701 gtaattcgct ttaaagcatg tactggtttg cagcgaccac cagctttctg aaacattggc
    65761 tttaacaact acttcgtttc gatcttcgtc ttgttattat taatatgtgt ctatcataga
    65821 gcgcaacttt ttctttgtca acagaaaaag gatatttttt ttaaattcct cattttttct
    65881 tttttgaatc cgctctaatt ccgaacattt tattttcaat tattgttaaa agaaataact
    65941 ttttctgtat tttttctgca atttatcgag aagtcacttt actttgcctc ttattctttc
    66001 ttcgttacaa taaggaatgg gacaagtaaa aaaatgaaaa agcaagggac gaggtatatg
    66061 gaaaaaaagc aaaaaattat tttaggagcc acttctgctt taactatcgt tattttaatt
    66121 ctaagtttgt tttttgtcat ccgatcatct acaaaaaaag aaccaatcat tcctgaaaaa
    66181 aaaataacag aaaaagcaaa agaaccgaca gatgataagc aacaactagt tactcaagca
    66241 aaaaatctgg ctcaaagcta cgattatgac caagcgattg cgttattgga aaaagatagt
    66301 aagaacggat ctactgaaac acaacagctt cttcaaacac taaaaaagga gaaagaacaa
    66361 ctgatcgact ggtctgatcc tactcagatc tctcatgtct ttttccacag cttgatcgtg
    66421 gacccgcaaa aagccttcca ctctcaacag gcacaaggat acaaagatta tatggtgact
    66481 gttgaagaat tcaaccgttc gatcgaacaa ctttatcaaa atgattttgt tttagttaat
    66541 ttgaatgatt tgatccaaaa agacgaacaa ggaaatcttt cctttaacgg gcttgcgcta
    66601 cctgaaggaa aaaaaccatt ggttctttca caagatgatg tcagctatta tgaatatatg
    66661 gataattccg gttttcctag ccgattggtc gtatcaaata acaatgaaat caaaaatgtc
    66721 tataaagaga aagataaaga agaaatcggt gactttgaca tggttcctct gatcgatgcc
    66781 tttgtcaaga aacatcctga tttttcttat cgaggcgcaa aaggtacctt ggccctgaca
    66841 ggttacaacg gagttttagg ttatcgaaca tcaaaatctc aatatggaga caatgaaaaa
    66901 acaaaaaaag agattaaaga agctaaaaaa gtggcggaac agttgaagaa agacggctgg
    66961 agctttgctt cccatacatg gggtcacttg aatatgactc aagcttcact agaagatgta
    67021 aaaaaagaca atgaattgtg gcagcaagaa gttgcaccta tcctaggaaa aacaaatatt
    67081 ctgatttatc catttggtgc agacatcagc gactggcagc cttattcagc agaaaatcaa
    67141 aaatttgctt acttgaagag tcaaggtttc gatattttct gtaatgtcga tgcttctaca
    67201 ccagcttggg gacaattagg tacggatttc tatcgaaatg ctcggatcaa tatcgatggc
    67261 attcgttttc aatctgattt agacggaaaa aatcctattc ttgatccatt catcaatgtc
    67321 catgatgtct atgacaaaga agcacgcgaa tctgcttgat tttacgtcat ttatgaacca
    67381 aaaaagccga actgacattt tatcgtcagt ttcggctttt ttatgcttta tttcttcttc
    67441 ggtgcgattc ccttgatcag gacttctgtc cattctgctg tatatggaag cagttttgcg
    67501 tatatctccg gcttagaaat tccttctacc attgcttgaa aatagaggat caagaactca
    67561 tccgaatatt tcaaatccac ttttccttct ttccgccctc gatggaaaag atcaagcatg
    67621 atactgaaac tttcttgcgt atacgcatcc atcgcgtcag aaaattcttt tttttctttt
    67681 tttgtatagt atgtcatcaa atcgatataa aattgtttgt tcactcgatt gagataacgg
    67741 attttgtttt cactcatggc aatcagtgtt tcttcaaatg gaagattttc cgcaatcaac
    67801 gctttcgctt cttgccctaa atcattcata aatgcaaaaa atacttcata aatcagccct
    67861 tccttgctgc caaaatattt aaaaatcgtc gctttactta cctgagcgtt ctcagctacc
    67921 ttatccattg tcactccagc ggttcctatg tctgtgttca tcaagtcaaa agctgcttgt
    67981 aaaatctctt tctttttctc ttgtgctctc ttttcaaaac cgttcatttg tagagcctcc
    68041 cgtaatttta aacttttata atatgtatag ttcaaattta ttctgaggtt tattataact
    68101 atctattgag aaagtatcaa gctagcaatt ccttgtttat gaattcattg acagaaattt
    68161 ataaaaagaa tattatgtac taaaataata aaaaaagttt tataaaaagg tataaaacct
    68221 ttttattaaa ctatttaatc ttgtttagta tatattttta gaaaaaggag tggggttttt
    68281 atggcagaaa tcgtcaaagt cacacatttg aaaaagagct ttggtcgttt cgaagcttta
    68341 aaagatgtct ctttcaccgt acattcaggt gaagtcgttg gatttatcgg accaaatggt
    68401 tctggaaaat ccacgacgat tcggacactt ttagggatta taaaaaaaga cggcggaacg
    68461 gcaacgattt ttgggcaaga tgtatgggaa gatagtttat ctatccatga acgtctttct
    68521 tatgttccag gcgatatctc tttgtgggga aatttgactg gaggagcgat catcgatctc
    68581 ttcatgaagc ttcatggaaa agggagtgct caaaaaaaag atgaactgat tcaacgtttc
    68641 tctcttgatc ctaagaaaaa agcaaaaaac tattcaaaag gaaatcgcca aaaagtgggc
    68701 ttgattgctg ctttggctgt tgattcggat ctctatttat ttgatgagcc tacttctggt
    68761 ttagatcctt tgatggaaac gattttccaa gaagaagttg aaaaattgaa agaacagggc
    68821 aaatccatcc tactttcttc tcatattctc agtgaagtcg agcgactagc ggataaagtc
    68881 gtgatcatac gcgaaggaac actcgatgaa gaaggaacac tcgatgaatt gcgtcatttg
    68941 actcgttcga ccatcactat agaaacagaa gcagagaata gtcacttagc acaattgaac
    69001 ggtgtccatg actttgtgca aaaaggtcac ctcgctcaat ttgctgctga taacgaagct
    69061 ttcgatgtga ttttagctga agcgtccaaa ttaggtgtga aaaaatttga atctattcca
    69121 ccgactcttg aggatttatt catgagacat tatgaaggat gaggaggaag cgaaatgact
    69181 gataaatttt cacgctggtc cacccttttt tctcaaaacc ttagccgtga ctggaaaaag
    69241 atcctgatat ggctagtcgg tgtcactgct ttttctggtg gttttgtccc tgcgtttgaa
    69301 gaattgactt ccggacaagg attagctgga ttatacgaaa ccttgcaaaa cccggcaatg
    69361 attgcaatgg tcggaccaac tccagcttcg aatgctgctg agtatacttt aggagcgatg
    69421 tatgcaaatg aaatgttgct tttttgcagt ttgtttgctg ctgtgatctc tgttttacac
    69481 gtggtgagcc aaacgagaaa aaaagaagaa cttggtctga ttgaacttat tcgttccttt
    69541 cgagtcggac gtcaagcaaa ctctttagct gtcatcgctg aaagtattct gatcaatttg
    69601 cttttagcaa tatcgatcag ctgtacgatg atttttttcc aagcggattc gatcactgca
    69661 gaaggtgctt ggctttttgg gctatctgta ggtagtatgg ggattttggg gagtgccgtt
    69721 gcactgtttg ctgcacaact gatgccaagt tcagccagtt cgaatggtct gtctttagcg
    69781 tttttaggtg gactttacct tctacgagca ataacagatg tagctgattc ctccctttcg
    69841 atgtggaatc ctttaggctg gtcttatttg acctatccat ttacaactaa caactggatt
    69901 cctttgcttt atttgttttt gttcagtcta atcttgataa tcagttcatt tgttttagag
    69961 gaacatcgag atttgaacac cggctatatc cccgaacgtg aaggaaaacc gtatgctaaa
    70021 cgttctcttt tatccttgcg tggtttatta ttctatctaa ataaagggat gacaatcagt
    70081 tggcttatca cttttggttt gatgggggcg gcatacggct ctatctacgg gaatatgcaa
    70141 cagtttttag aaagtaacga cttaatgaaa cagatgttta tccaatctgg cgtctcaatt
    70201 gaaaaaaact ttacaagcac gatcatggta gtcttgattt gtctagctgc tattttacca
    70261 attgctagca tcaatcgttt atttacagaa gaaacccacg gacgtatgaa tcagctgttc
    70321 gcaacaaaaa tttctaggaa ccaactttat tggaccaata tgttactagc tattctattt
    70381 gctatattgg caatcctgat tagttctggt gcactagggc tttctgcttt ggcttcgatg
    70441 aaggatgcga aagacatgga tctattgatg tttcttaaag ccgggttcaa tttcctgcct
    70501 gccgttttag tgtttatcgg aattagtggc tttctattcg gatggcttcc tcgaatcggt
    70561 aaacttgctt atatctactt agcttactcc tttgcactca attactttgg cggaattttg
    70621 gatttaccag aatggttttc caaaacagct gtccctagct gggttccacg gatgccaaac
    70681 gattcttttg atctttccgt gttccttacg ttatcatgta tcagccttgc actgttcgtc
    70741 cttggttcta ttggttatag gcgaagagac ttactagaag gataataaaa aagcatgtac
    70801 aatttcatgc tagcctccaa atctgggtca aaaatccaac atttggaggt cattttttta
    70861 tttacaagtc ttctaactgc tctttatcaa tgatccagac ttctcgattc tttcttttta
    70921 cataaccatt ctcctctaat aatttaaatt ttctagataa agtttcgggg gtcgtaccga
    70981 taaatgcagc aagttctttc attttcattg gcaggaaaaa gtgatccttc cctgtcgctt
    71041 tagctaagtc aagcagatag acagcgactc gttcttctac tttttccatc atcaaaaaag
    71101 ttgtctgctg ctgtactttt gttaatttct caacgttgat tgctaaaagt ttcagactaa
    71161 gggcgggata atgcagtaag agttccgaga aatctttttg tctcaatatg caaatttccg
    71221 ttttttctag tgcttcgcca aataaagact catttggaac gcctagcaac tggctctctc
    71281 cctcatatcc gcccggctct attactcgta aaagctgttc ctttccgcta gcagataatt
    71341 gatacacttt catactgccg gaggccacga tcactaattg aggttctcca ttcggcgcaa
    71401 gaatctgttc tcctttttcc acatagtgat gtttgacaag tgcgtgtact ttttcttgat
    71461 cttgctgtac taagtgattg aacaaaggaa caaatgaaac acataaatga tcatgactca
    71521 taatctctcc tccttctaaa cagctatttc taataaagaa acataacttc ccttattcca
    71581 actgataatt ttcttttatc atagcataaa cgtcaagcga accagatacc agcaaataga
    71641 gtggatttct ttttgtccct tgctttattt tccttctttt gagaccttaa gtaattgatt
    71701 cctatcaatt ttttaaatat ttactttttg aataatcaaa aacaaacctt cactcgcaag
    71761 ccgagagtaa ggtttgtttt tcgcattgct tatttcgtgt tgtctattga aatttttccg
    71821 cacctagctt ttgatatagt ggtgctagtt cttgtaaggt ttcagcggtt atttgctcaa
    71881 cgtgttcgat ttcttgcgga tagaagaata gtttacccat ttgaaaggtt cggtctctca
    71941 tcgctttttt caactcttct tgtttctttg tatcggacaa atagtcagac aaagctaagt
    72001 ggtcgtcaag ttcatgttct ggttgcggcc atatgtaata atccttgata tcgactgttt
    72061 tgctccactc tttcagtatc gcaagcaact ggttatacgc tgcaatgcta ccatatcgtt
    72121 cttcgctttt ataatgttgg tacatcaaat agatttggta ttgtttttga ttcaacaaaa
    72181 ctgctagaca ggcattctcg tcttgacgag attcgcttcg gtacgcgctc caaaaatgac
    72241 tgcgtaaatt ccagccattc gtccagcttt cgattttagg tttgctcatg ccgtacatat
    72301 caggcaaaag tgcagctgtc tggagctgaa tctctttcca tttttgccag tgttctttat
    72361 attctttctt gatttgttca atcttatctg ttggatggct ctctttgagc tgtttgaatg
    72421 cgtagattgg tcgatcaaac aactgataat ctgattttcc aagttccatt gtttcgcctc
    72481 tttccgttat cttttgtatt ctacctgaat tttgcgatat ggacaaatag aggttgtgaa
    72541 aaagctgttc tgcatcaagt cataagaaca gccaaacaaa aaactgcgat gagcatcacc
    72601 gcagttttat tgaacttaag gttgattgat cacatctaaa agcgttgaaa ataaaatggc
    72661 actagaagca gctcctgggt cgatatgtcc gattgcacgc tctcctagat aggaagctcg
    72721 tcctttttta gctaccaaga ctttggtgtg ttcttttgcc tggtcaatga tttcttttgt
    72781 taaattccct gccttcaatg cctctgtgac tggcagccaa acatctaaca tcgttttatc
    72841 gtcagcttct gcctttcctc ggctttgaat acccgctgtt ccctgctcga tcacttcgcc
    72901 taatttttct tgagaatcaa tagaatcgac ctcttttgtc gctttcatca tgttcatgaa
    72961 tgcagatccg tataacggac cagaagcccc accgactttc gatatcatcg ccatagagaa
    73021 tactttgaat gtatcgctga ttgtttcagg atgatcggct tgaaaagctt cttcataagc
    73081 tgaaacccct cttcccatgt tatttccatg gtctccatcg ccaatcgggg tatctagatc
    73141 actgagatat tgtttgttct gattgattgc ctctgcaaat tgcgtgagcc attcttggat
    73201 ttcttgaacg gttagtttca tattatcacc cttttatctt gagatttttt accatgaaat
    73261 cgtctgtaca ttgctttcta aagaatcttt ccattgtgtc tccgtcaagt cgatcagtgt
    73321 tagagaaaga ccttgcatat ctagtgaggt cataaaatta ccgacttttc ggaaaactac
    73381 attgacgcct ctttcttcca acagagctaa aacatcattc atgaacacga actgttccat
    73441 caaaggcgtc ccgcccattc cgttgactag tacacctgct tcttttggta aattcgagta
    73501 atcatctaaa atcttagtaa caagttcctt tgccagtact ttagaaggct gcatcttttc
    73561 tcgacgatag cctggctcac cgtggatacc aacaccatac tcgatctcat cttctcctag
    73621 ttcgaatcct ggtttcccta cttctggtac agttgctgct cgaagcgcta gaccgatcgt
    73681 cttagttgcc tgtacgacct tttctcccag ttctttcaac tcagctaggg aagcaccgtt
    73741 tcttgctgca tcgcccacga ttttgtgtac aaggacagta cctgcaactc cgcgttttcc
    73801 agcagtatac gtactatttt caacggcgat atcatcatct acgatcacac tttctacttc
    73861 aatatcgtcc atagcagcta attcctttgc catatcgaag ttcaatgcat cccctgtata
    73921 attcttaacg atcagcaaaa ctccttttcc actatcggct tccttaatag ccgtctgaat
    73981 ctgatctgga gttggagaag taaacacatc accaagcacc gctgcactta acatcccttc
    74041 ccctacaaac ccggcatggg atggttcatg tccgcttcct ccaccagaaa ccaaaccaac
    74101 ttgttccgct ttctgattct tggctacaac acgggaagat tctacacgat gaacaagttc
    74161 tggatagctt tttactaatc cttccagcat ttcttcgaca atattgcttg gatcattcat
    74221 gatttttttc aatttattcc catcctttct ttccggcatt ttgtgcttct gttaagattt
    74281 gctgagtatc atctgttata ccggcagtga tggctgctgc aaaagctcct tccactaaag
    74341 gcgcatccac cagatgataa cgttgctgct gctcttcttc caacatgtct tttgccagtt
    74401 ccgagttcaa taccgcgcta ccgatatccg caaaaacaaa ataactagct gcatcgcttg
    74461 attcattgac tgcttctacg attttcatcg ggtctgatcc gatctcgcca tttattccgc
    74521 cgagagaaaa aatttgcacc tcttcggatt tttgcatctg ctcaatcatt tctttaatac
    74581 catctgttac tttttgacta tgagaaataa gcaaaatact ttttttcaca agaatcttcc
    74641 tttcttttac atagttgcta acgattggtt tcgctttcat taatgaacaa ttcaagtata
    74701 taataaattt ctaattttgg gaaaatatgt gtagtgcttc ttgaagtttg ttatacttta
    74761 attaaaagag aggagtgatc gaatgattac aacaacaacg aacagtgtag agggaaaagc
    74821 cgtagtagct tataaaggga tcgtttttgg tgaggtcatt actgggatcc atgcatttaa
    74881 agacttggag gcggggttgc gaaatatctt tggcggccgc tcaaaaagct atgaaaacga
    74941 actaatgggt gcaagagaag aagcacttgc cgaaatggcc gaacgtgcaa aagcattagg
    75001 agctgatgcg atcattggcg taaaaatgga ctatgaagta ttaggcagcg acaatggcat
    75061 gctgatggtc acatgtagtg gaacagctgt ctccacgaga taagtagggg acatctgttt
    75121 taataactaa agagagatgg gacatgtagt ggcactcaag aattagattt ttcagtctaa
    75181 cttcgaggta ctctaccttc ccatcctctc ttttttattt ctttttgcgg atgattccgt
    75241 aagctgctgc gactaacaca cccgcccctg ctaaggaata actgacagaa gctgtttttt
    75301 catttgtttt tggcaaagtc tgacttgttg atttggatgg ctctgtcgtt tgctgaactg
    75361 tttctgtcgt gcttccagag gtcactgatt tttctcctgc cgaatgcaaa atatctttta
    75421 ttcgttggaa actagctgct aagtcagctt tttttgcttc aaaatcatta gcaactgttt
    75481 gtgcttcgct gatctttccg tggattgctt gactggccgc tgtgatatcc gcttgagttt
    75541 gacttagctt tccagaagct gtttgagcaa cctgatttaa ttgctgattg aattcttttg
    75601 cttgttggat cttcccgtca agatctgttt ggacagcttc cgcttttgct ttcgcttcgg
    75661 caagcttttt ctcaagttgt gcacggtcat ttcccacttt atttttaaga tcagctaaat
    75721 cttgatcgat tgctgcttgg tcttctttca atttgttttt tgcagcttct aatgcttctt
    75781 cgagttcttt tcttttattt tccaagtctt gcaaatgatc gatttggtca gaagaggtag
    75841 aactatttat tgatgtatct tctgaagatg aaatcgtttc tcctgaatta tctgtcggta
    75901 cattggaagt catttctgtt tccgaggcac cgttttcagc accagaagag cttatggttg
    75961 ttgaaggttc tgtttgagca ggttcagaag tttccgcaaa agctgtctgt cccaataaga
    76021 aaatactgga aacagcaatt ggccagatta acggcttcat tctttttttc atcacttttc
    76081 ccatccttac tactttttaa ctaatttctt attataagca aaaatgtttg gaagaaccat
    76141 aaacacaaca ggctgtttgt ctaaaaaacg gtgcatcttt tatttttttg tacgttccgg
    76201 gcttttccat gtcccgcttc cataacctaa aatcgttttg actgctggaa tgaaagtcgt
    76261 cacgatcgtt atcgcgttca ctatccagaa caaaagcata tatagtggtg cgaaaagaag
    76321 gtatttcaat tttcttcgtc gatcatctac aatcagcgaa gctagcaact ggaaaaatcc
    76381 tgcgaccatt tcaaagcaga tgaacacaaa actgaatgta aaagtaatat aaatctgttc
    76441 gtagtttcct tgatatacat aatgcccgat taatccagca aaaagaacta cggaaataca
    76501 aaagaatatt gaccagacaa tactcaatgt ttgatcaatg aagatgatcg ttcttccaat
    76561 atgtttaaaa ggatggagca tcactttttt gaaattcgtc agccagactt ctgttccacc
    76621 ttttgcccaa cgttttctgt gtcgatatag catcttcaac gtcacaggta cttccatgaa
    76681 aaagataatc tcaggagcaa aaacagataa ccagtcattt aactgatgat cccaagcaat
    76741 actgatgtct tctgtcgctc gatcttgtct gaatccgccc acatcgatca aggcttcttt
    76801 tcgatacatc gtatttgctc cactgtacgc ataaagacca ccgaaaacag ctgactgtgt
    76861 tcgtttaatg atgcccacga tacttgagaa ttcaaccgtt tgagattttg caatcaattt
    76921 ggtacgattt tggacatcca tatttgcagt gaccgcagca atatgtcttg caccagggcg
    76981 aatgaaataa ttcacatacc taatcagtgc atctggctcc ggtaccgtgt ctgcatcatt
    77041 gcttaaaatc aatttccctt ttgcaaaggc aagtccgata ttaaacgcgt gggcttttcc
    77101 tttatttttt tcgatccgaa ccacacgaag attagcgtat tttttcatca gtctagctaa
    77161 tatttctggt gtttggtccg ttgaaccatc gtctgtcact aatacctcat agttgtgata
    77221 attcaatttc gtcatcaaat actcgattgt atattcgatc acaatttctt cattatgggc
    77281 tggcaccatg atcgtgatga acggttcgac actcaaggga acatctaccc attctttttg
    77341 cttatgtttg tataaaaaca cataacacca gacaccaata aaccatacaa atccgcctaa
    77401 aatcggataa gaaacgagaa aaaaatacat aatagacaaa caagtatcta agaataaatt
    77461 acctgtatgg gtgattgaga tcatttcagt tccaccccat tttctttata caaatctgca
    77521 atgaatgtcg tatctagatt tttctcttcg gagacagaat aaaaacggac cgtttctctc
    77581 tcttcttttg ttccaaaacg ttccgtatac acttcttcaa gtaattcttg tctttttttc
    77641 aatttttcat cttcatattc aaaagattta tttacttttt tgtatagatt tcgattgttc
    77701 cacctagtca atacaaatga ggtaatcagg aaactgaaaa aaatcaatac aaaaaagccg
    77761 attaagaaat acagcatttt cttttctgat aaaaaagcgt acaaggaaaa atccttggct
    77821 ctttctggtc tcgttaatgg gaaaaagacc catataaaag gaagaacaac gatcatccat
    77881 cctaaaagag ccactaacgt ttgccaaatc tttaaccagt aattaccttt tgaaaaatac
    77941 ttatctttca ccaaaattga tgcaacttct tctcttgtta cttttttcat atcctcacct
    78001 cacgtttttt tgttacgacg taggagttta ccatacattt catttctttt cacataaagt
    78061 gttcctaaat ttttcctaag aaaaagaaca aaacatttct catcagcaaa tgaaaaatat
    78121 tttgttcttt ttatcagttg ttctttttat tttttatctc ttaattatgt tgctctttcc
    78181 aatacgacaa tgcttttcat tcctctagat atcgccgtgt acatttgtct gcgtcttctt
    78241 tgctcatctc catcgttttc aaatggatat aagattacat gatcaaattc cagtcctttt
    78301 gctagactca aaggaatgat ttggcagttt tctaatccat atgaattttt tttaatcgat
    78361 tcttgtagcg tttgggtttc ctcttctgtt tgggtgataa ctgccgcttt ttctcctttt
    78421 agagaaacca aaatctcagc tagtattttc aacaattctt cagaggatcg aaaagagcgg
    78481 tattcgggtt cttttccttg cggtcggatc gatacgatgt cgatcttctc ttgatccaca
    78541 gcataggttt tgaaaagctc tgtgattgct ccgctagaac ggtaactatt tctaagatca
    78601 taccgatgga tcggcagatg gtagtcgtcg aaacaacgca taatatcagc aaaagtagtg
    78661 gaagaattaa agattgcctg attctcgtca cctactaaag taaagtgagt ttttggaaat
    78721 aattcgatca atagacctaa ttgtgccagg gtataatctt gtacttcatc aatcaataga
    78781 tagcgaaatg ctggtacatt taccttttca acaagtaaat gacgaatagc taatataatg
    78841 actgcttcat caacagtacg tgtgggttgc caagcataag ctgagccata tcgtttttca
    78901 taaattcttt caaacaactg atgctcatcc acccatgcca tttcttcgat ttgtctggtt
    78961 atttttcgat attttttttg taaaagtttc cttgcataag ctacaatact ttgttccgaa
    79021 tcatcagaaa tcaatttacc gaataattcc aactgcctat cttctgtcaa cgagagtacc
    79081 tgattctgca cagcaggact ttttgcttgt ttcaaaagat gattcttcca ttcgctcatc
    79141 agtgcctttt tcgttgcttg aagcctatcg atcaatttag gatgagcagg aactttctca
    79201 tatatttttt cgatcgtttt tttagaaagg actgtttttc catttttagt aagtccacgg
    79261 ataaaattcg gatgatctaa aaacaaagga tctgattgtt ttagattatc gataaacttt
    79321 ttgctacgca aacgttctgt ctgctccgat acattttctc ccgtgatacg tttgaaataa
    79381 gcctcttcac cttctatttc ctctgctgaa agctgtgaaa ccagctgtat gatggttaga
    79441 ttcagtggat ttctttcacc taatgatggt aggacattcg aaatatagtc gataaaccga
    79501 tgattaggtg atagaatcag caagtcatcg gatgtcatcg tatctcgata taaatagagc
    79561 agatacgcaa tacgctgcat gatagcagag gttttaccac ttcctgctac gccatttacc
    79621 aagatatttt gacttttcac atcccgaatg attgtgtttt gttccgattg gatcgtagca
    79681 gtgatagcct tcatttcatt ggtctcatct tggcctaaga cttctaataa tacgtcatct
    79741 tgaatagcta cagacgtatc aaaaaacttg accaaacgat ctctttccaa aagaaattgt
    79801 cgtctttctt taataaccgt atcgatttta ttttgacgaa ccgtataaga agatggacca
    79861 atcatgttat tgtaaaaaag ttcggcaatt ggtgaacgcc aatcataaac aagattttca
    79921 tctgcatcat ctgtgaaatt tgctgtaccg atataaaagg attcttttgg ctcattatcc
    79981 agaaagtcta caacgacttt tccaaagtag ggacttttca ataaacgttc gatctttgct
    80041 agctgatttt ctgcgttttt ctgtttgata ttcatctgat cgatttctct atttttcatt
    80101 tcaatcatcg aaaaagtatc caggttatct aagtaactgt caaaattcaa tcgaagctcg
    80161 ccagccattt ctttcaacga ctggtttcct tctatttttg ccgtatccat cgattcccgc
    80221 agttcgtttt ttttatcaat tagcttttga tacacgttct tcagatatgc ttcttctaac
    80281 tgtttttgtg tgttgaccat acacattctc ctcttttttt aaagtggtgg aaccgattag
    80341 tttacctctt ttaagagtcg aatgcaagct ttttaacgag tatcatttgt taggatatat
    80401 cagttcttca ggtcggacag tttgatataa agaaaatgca ccatcaaggt tttttacgtg
    80461 gtagccagac tgcttcaaga ttctttccgc tagatagcta cgtaatccgc tatgacagct
    80521 gacgatatat tcttgttggc tgtctaattc atttaatcgg tcccgaagtt cattcaaagg
    80581 gatatgtttg gcttccttga atttgccctt ttccaattct tctgctgttc gcacatctaa
    80641 taaaattttt ccttttgcta gttctgtcgg caattcatgc cattgtacgg tttcactaag
    80701 cccttctgca atgttcattg ctgcatatcc tagcatgttt actggatctt tggctgagcc
    80761 aaatggcggt gcatacgtca attctaattc cggtaaatcg aagatcgtca aatgcccttt
    80821 gattgctgtt gctaaaatat cgatccgttt atccacacct tttgccccaa caccttgtgc
    80881 accataaatc tcgccagtct ctggatggaa gatcaacttc agtaaaatat ctgttgcttc
    80941 tggataatag cttgcatgat cttttcctga tacatgaatg acttcaaaag acagttcgtt
    81001 ttctttggct gttctttcac ttaaaccggt ggatgcggca gtcattccaa acgttcgtat
    81061 aattgctgtt ccaataccgc cttgatttct tcgagaaatt ccggaaatcg tgtccgcaac
    81121 ttgtctacct tgacgattgg ctggtgaagc aagagaaata agtgcatctt ttccagtgat
    81181 ttgctgtttt acaacgatag catcccccac cgcataaata tcaggaatat tcgtttgata
    81241 acgttcgtct actaggatac ccccgcgtat gcctaattcg attccagcgg tcactgctag
    81301 ctggttatca ggacgaacac caatggataa aataactaaa tcagcaggga tggtacgccc
    81361 atcatccagt cgcaatcgtt tcccgtggtc ttcaaatcca gcgacagcat gagaagtaat
    81421 cacttctact ccttgatgag acaattcttt ttctataaaa gctgccattt cttcatccag
    81481 tgttggaaga atatgtggtg cttgttcgat caccatgact tctaaacctc ttctttttag
    81541 gttttctgcc atttccaatc cgatgaatcc tgcaccaacg atcacggcac gctttggctg
    81601 tttttctaat gcatgcatca ctttatcgat atctggtaca tttctaatag aaaaaacatt
    81661 gtcggcttct gttatccctg gaatcgatgg aacaactggt tttgcaccag gagataaaac
    81721 caatgcatcg tactgttcaa tgtgtttttg accctcatgt acgacttcta ccgttttgtt
    81781 ttcgggaaag atggctgtca cttcatgatt tggacgcaca tctaaacaaa aacgagcgga
    81841 taatgattct ggcgtttgga caagaagatt ttcccgttca ctgatttctc ccgaaagata
    81901 ataaggcaag ccgcaatttg caaaagaaac atacggtcct ttttcaaaaa caacgatttc
    81961 tgcatcttcc attaatcggc gaagccgtgt agcagcagac attccacctg ctacacctcc
    82021 gacaataacg attttcatct tatttccctc ctactgttcg atcaaaccat tgattcatgc
    82081 caccacgtac attcactaca tcataaccag atttctttaa ttctttagct gcttgtttac
    82141 tcctcatccc tgattgacaa atcacataga cggtcttttc tttgtctcca tcatatctgt
    82201 ttatactttg taatggaaca tttttagctc cttttatatg cccgccccgg tattctgcag
    82261 gtgttctaac atcaagtaac gctggatttt ccaaaagctt ttcctttaat tctgggactg
    82321 ttatcgattt tattgattga aatagcaaat tcattttctg cacctcattt acatttttat
    82381 aaatacctca tggggtatat taaacaggag ctttattaat atgtcaacca gaatgactct
    82441 tcattttttt cactactttt tatatttgca taaaataccg ataaggggta ttatctaatt
    82501 ataaaaatag aggagaatgt gttatggaga ttgataataa aaatatcctg aaccgtttga
    82561 aacgtacaga gggacaaatt cgtggtatcc aaaaaatgat cgaagacgaa aaagaatgta
    82621 tggatgtcat cactcaatta agcgctgttc gttctagtat tgatcgcgta atgggcatga
    82681 tcgttgcaga taatctgaaa aattgctttg agaaacctga tagtaatcca gaagaacaag
    82741 cggctaaact agaacaagcg atcaagatga ttattaaaaa gtaaaggaat gatttcaatg
    82801 aaagtcgttt tccatattga tgaattagaa aaatggtcag aaacagggaa aaacgtaaaa
    82861 aacctgataa aagcttctcc agaaacgacg attgtcgtat ctgtaaatgg catcgcgatt
    82921 accggttatc tagactctgc caatgcagaa ttcttagatt tgcaaggagt cgtttttcac
    82981 gcttgcgcca atgctatgcg tgctaatcat atttcagaaa gttctttacc tgaacaagta
    83041 atcgttgtac cagcaggtgt ccttgattta gttgaactgc aatcccaagg ttatgcttat
    83101 atcaagcctt agtcagtaga ggttgcgaca gaagtgatca gcttcaagaa atttcggctt
    83161 atttttcgaa gaagctactt ctggaaaacc gtgaatcaaa ggttgtgaaa acgctgtcct
    83221 gcatcaagta ataagaacag ccaaacaaaa atagcttctc atatttttcg tttggttggg
    83281 cttattaccg cagatgcaag cttttgaaca ccgtttaagg aagaggttgt gaaatcagcg
    83341 gtttgaccta agtattagag aaaaaaacgg gaaaatagct cctcatattt tgccgttttt
    83401 tgagctaata ccgaaggtca gctgattgaa caccgtttaa ggaagagatt gtgaaaaagt
    83461 cgcttagttt cgagtattaa gaaaaatttt gtgaaaatgg atttctacat ttttgcataa
    83521 atttggctta ataccgagaa actggctttt gaataccgtt taaggaagag gttgtaaaat
    83581 cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagctgctca tattttgccg
    83641 ttttttgagc taataccgaa ggtcagctga ttgaacaccg tttaccaaga ggttgtgagc
    83701 ctaccattta actccgaatc acaagaaata atttcaaaaa caacttctcc tgtttttggt
    83761 ttggttggct tattatcgag aagtagctac tgggagtcta tttatcaaaa aaaggaatgc
    83821 gacataggtc acaccccttt tttattttct ttgaaacatc tttcggtact cgacagcata
    83881 caagacaaaa gattgtaaaa gaccgtaagt gatgacggca aaaatgactc tcacccacag
    83941 cgttggtaat tctgggaaaa gcagcaaaag gatcatgcca acatagatga cagcagtcgc
    84001 tactttgcca taccacttcg ctccacttaa tttgatattt ttagatagcc cgattaatcc
    84061 agctacaaac ataaagcctt cttttatcaa gaatagacca aacaacagcc aaagccacgg
    84121 tcgataccat gccatagaca aaatcagaaa aagttgtgtc aatttatctg caaacggatc
    84181 aagcaccttc cctaagtctg ttacctgatt atatctccga gcaatgaccc cgtctagata
    84241 atcagacaaa cctgagacaa caatgaccgc tcctgctata taaaatgact gtctcagcac
    84301 aaaataaaga taaatcggca gcaaaaaaat acggaatatc gataaaagat tcggaatcgt
    84361 ttgtatttct ttacgccagt ctgtgcttaa tttcatttta tcgccctacc tcctcagcac
    84421 tcttttcttt ttattttagc ttacacgggg aataaaacca atgatttcac tgtttttatt
    84481 tttctttaca gccactgata ttatgagcta atttgagata atcacttgct gttttcatcg
    84541 tttactcttc tttcttcctg tcgtttgcgc atttctttta ctagtacttt atgagctttt
    84601 tttgatcctc gctctaggtc acgctgccgt ttacgtgttt tataatcgat aaacagactg
    84661 tctaaatcgt aacctgtcgg gtacagatct tctgctgaac gcaccagacg aacacgctga
    84721 cgaggcacgt ggattatttt tttgtctaaa tatacatcaa tcgtatcttc acctatatct
    84781 tcataaacta acgcttcttt ttggtattca ttgacaaata tccgatcacc ttttgcaaag
    84841 agctggcgtt cttttttatt ctccttccta tttgtaactg ctgtcttagg aagaaattct
    84901 tttttctgtg tccgataggc tttttccgcc aagtaacgct ctgcttgatg gatcaatttc
    84961 atcgacatat ccattttatg agcaatccac aaagcttggc tttctcctgt ttctccaacc
    85021 cgcaactgat atttaggacg aagtgcttcc cgatcgaaag ccatagcagc cggcacaaaa
    85081 tcttcatgat ctccggcgaa tttcttgatt tctccataat gggtcgtggc aatgatcaat
    85141 gccccttgct catacataga ttccatgatt gcgatcgcta gtgcagcccc ttcatttggc
    85201 tctgttccac tgccaatctc atctaaaagt accaacgtat tcctttttat ctttttcaaa
    85261 atagcagcga tgttttgcat atgaccagaa aatgtactca acgcattttc taaattttgg
    85321 tggtcaccga catcgacgaa aatctcatca aagacagcaa gctctgtccc ttcttttgcc
    85381 ggaacttgca taccgaacat cgccatcaaa gtcaataaac cgactgtctt caacacgact
    85441 gttttgcctc cggcatttgc tccggtgata atcaagcctc gatagtcttt accgatttca
    85501 acatccaatg gtaccgcatg ctcaggtaaa aatggatgtc tgccttgctt gataatgata
    85561 tgttccgatt tattgattct aggtgtgctt ccattgattt ctcggctaaa ttttgcccga
    85621 gcaaagatga tatctaacac cgtgatcgtt tcgatcaata aatcgatcac ttcttcgttt
    85681 tctgcgatag caccagtcaa tgccgccagg acttgatatt cttcactgat ttcttcagct
    85741 tttaacaact gatagtgttc attcaattta gaaatcacag ttggttcaat aaaaaccgtc
    85801 gttcttttat ttgattcatc gataatcgtt ccatctattt tatttttata gctggctttg
    85861 ataggaatcg tatagtattc gcctttttga acaatcattg cttcttggat catttctttg
    85921 tttttcggat gacgcaagaa tttcagtaat tttgactgga tttctttttc gattagttgt
    85981 aattgtttgc gtactttacg caaattacgt gacgcgtcgt cactgacttt attatttttt
    86041 attttctggt caatcaactc ttctacattt atcaaatcag gaagatgttt gctataagca
    86101 aacaacaagg gtgtctggta ttgattctta tcaaaaaact ttgtgatcat ccgactgctt
    86161 ctaagaaaat ccgcatattc gatcaaatcg gttggctgca gaatcaaccc ttttttgact
    86221 tgttctgtca gtgcatcgat cctcggcaac cccataaatg gaacatgctg actgctttct
    86281 aagatcaagc gtgcttctct tgtttcttct tgacgatcca agacagtttg taggtttgtc
    86341 gagaccgtca tatcgctgat tcgttttttg ctatagtttc caattgctct tgcttggatt
    86401 tcctttttga tccgatcaaa ttgtaattga ctaatcattt cgttgtccat attctctcct
    86461 tttcttgaag agatacctgt cccaaaataa ataagggcct agagataaat aaaaggatag
    86521 aacacagctt aaaaaagtca tgtcctatcc ttattagaat acaaagatat tgttttcctg
    86581 acaggtaccg tttcataagc acaccaaata aactttgttt attggctatt cgacttactg
    86641 attagatacc agctacactc acaaaacata ccccattctt ctaggtctta tttattttcc
    86701 acttaactat agcaagacac attttggttg tcaaacttta gtatgtttta tagatttaca
    86761 ccattaatga atagacacct accgcgataa ttgctccgat cattggacct gcgattggta
    86821 cccatgaata tgcccaatct gaagttcctt tattagctat tggcaatacc gcatgtgcta
    86881 atcgaggacc aaagtcccga gcagggttga ttgcatatcc tgttggccct cctaatgaaa
    86941 gaccgatggc taagatcaat ccacctacag cgaatacgtt cgttccaggt gcaaattcgt
    87001 tttgtccaaa agctagcaac ccaagaacta atacaaatgt accaattaat tctgtgatca
    87061 catttgctgg ataattccgt atagcaggac ctgttgcaaa cgttcctaaa atcgcgcttt
    87121 catctttcgt tgcattccaa tgtggcaaat aagaaagcca aacaagaatg gcccctgcaa
    87181 aagctcctaa aacttgcgca atgatgaaag gaacgacaag attccaacta attgcccctg
    87241 tcatcgccat agctagagag actgcaggat tcaaatgagc tgggcccatg aatccagaaa
    87301 tatagacagc cattgttaca gcgagtcccc aaccaaatgc gatgacaatc cagccagaag
    87361 cctgtgcttt acttttgttt aaattcacag ccgcacacac accatctcca agcaaaacta
    87421 ggatcagtgt cccaataaac tctcccatga tttgagtcat atcactgttc atttatttct
    87481 ccccctttag ttcccgcaga tctgattctg caatcaatgc ttctaaacgt ttagtctgtt
    87541 gcgccttctc ctcttctgtc cattcaaaat atccagcaat tgcatcgatc acaggttgct
    87601 tgatctcatc aagctgatct cgttcaaaca acagatgatt tgtacgacga atgagataat
    87661 cgcctggggc caaaaccatt tcttcttcta aaccataacg taaccgagct gactcagcga
    87721 gactcaaacc aggataaggt gccatttctt ttgctaattc aaagatacgt cgggcattag
    87781 tcccgtaaaa atcagcgata taagtagcat cttcttcctc taaaccggct gctactcctt
    87841 cttttgctaa ttctgtcact gtttcttcta atttagttgg atcgaagttt ccacctgaaa
    87901 tctgatattt tttagagtcg atttctttcg tctctattcc gtattcttct tttaataatt
    87961 ggcgtatcaa tcgcaaagct ccttctgcca tcttacggta atcggtgatt ttcccaccag
    88021 ataaagtcag caaaccatct ggttctcttt ctaaagaact acctctggaa atcgtagaag
    88081 gtgctttttc atcacggctg ttttccaaat ggttcaacac gtcttctact tcagcacgag
    88141 aaactttgtt ttccttatat tcacttactg tatcgacaac tttattgaag ctcttgtctg
    88201 aaatcgatcc attatctcca ccattgtaat cagaaccaga attaccaatc aacagtggac
    88261 gaagtcctgc ccagcttgct tcgatatcat tcaatgtgat atttgcttct ggatagcgat
    88321 gattgatcac atccaataga taatctacat cttcttgtgt gactttcgga tccgtaaaat
    88381 ctccttggta atccgtatct gtcgtaccaa agtaagtctt gttttctctt ggaatagcaa
    88441 aaaccatccg cccatcttgc tttcctgtat cgaagtatgt gggttgcggt acaggcagtt
    88501 ttttcgcatc tacaactaaa tggatccctt tagttggacg cattttagga gagactggac
    88561 gcgtaaaatt caagtttcgt actttatcta cccaaggacc actcgtattg atcactaatt
    88621 ttgctttgat ttcgatcact tcatctgtca gcaggtcacg agctttgacg ccaacgattt
    88681 gatcgccatc atataaaaag ccaaccgctt tcattttact tactagataa gccccatctt
    88741 cggcagcctt tttgatatta tcaatcacta aacgggcatc attgttgcgg aaatctagat
    88801 agacacccgc gccttttagc ccttcttttt tcaaaaacgg ttctctttcc aatacttctt
    88861 ctggtgtcag cgtataattc tcatatttgg ttcctgtcac atttgccagt ttgtcataaa
    88921 ggtccattgc tactttgacc gagaacatat tgaaggttgt tgctccttcg ccctcataga
    88981 ttggcaaaag cattggatct ggttttggga tgtgtggggc aatactttgt acgactgcac
    89041 gttcaccaac tgtgtcagct actacttcca catcaaatgt cttcagataa cgaataccgc
    89101 catgaacaag tttggtcgag cgagaggaag tcccttctgc aaaatcctgc atttcgatca
    89161 atcctgtttt gattcctgat gctgctgact ggactgccac accggcacca gtgattccgc
    89221 caccgatgat caacagatcc aattcttctt gctgcatttt ttgaatgcta tcttgtcttg
    89281 tcttgtttga aaacatatgg atttcctcct tattcaaatg cttgggtagc ttttactgct
    89341 tttttccaac cgttgtaaag tttttctcgg cgctcttcct ccatttgaac ttcaaatagc
    89401 ttgccagctt cataaaattc gcggatctcg tctgtatctt tccaataacc gacagctaat
    89461 cctgctaaaa atgctgcccc tagcgccgtt gtttccaagt tttctgctcg ttggatcggt
    89521 acattcaaga tatctgtttg gaattgcatc agatattcat tgtttgctgc accgccatct
    89581 actttcaata caggaatctt gattccagtg tcatcttgca tcgtatcgat gatatctctc
    89641 acttgataag caatcgactg taaagtagct ttaatgatat cttcttttgt cgtaccacgt
    89701 gtcaagccaa acatagcccc tcgtgctgcc tgatcccaat aaggagcacc tagcccaacg
    89761 aatgcaggaa caacataaac ctcatcttcg cttgtagagc gtttcgctgc gtcttctgaa
    89821 tcgctagctt tttgcaacat ttgaagtcca tctctcagcc attgaatcga agaaccagcc
    89881 acaaaaatac ttccttctaa agcatagtaa acctttccat ttatgccgta accaattgtc
    89941 gtcaataaat tatttttgga taattgcggt ttttcaccgg tattcatgac aataaatgaa
    90001 cctgtaccat aagtattctt gaccatgcct ggttcgaatg ccatttgtcc gaataatgcg
    90061 gcttgctggt ctcctgccat ccctgaaatt ggtgcttcgc ttccataaaa atgataacct
    90121 tgcgtataac cataaacttc tgagttagaa gtgacttttg gtaacatcac tcgaggaata
    90181 tttagtaaat ctaaaatctc ctgatcccaa tctagctgat ggatattgaa caacatcgtc
    90241 cgactggcat tcgagtaatc agtaacatgt gcttgtccat cggtcagttt ccatactaac
    90301 caagaatcga tcgttccaaa catcaattcg cctttttcag ctcgttcttg cgcgccttct
    90361 acatgatcaa gaatccagcg tatcttcgtt gctgagaaat aggcatcgat cactaaccct
    90421 gttttttcgt ggaaaaattc ttgatagcct tcatttttta agttatctgc aattccagct
    90481 gactgtctgg attgccaaac aactgcatga tagattggac gtcctgtatc tttttcccat
    90541 attactgttg tttcacgctg gttcgttatg ccaatcccag caatctgatt tggtttgatc
    90601 cctgattcga tgaacgctcc ggcaataact gactgtacag aattccagat ttcatttgca
    90661 tcatgttcca cccaactttc ttgcgggaaa tattgcgtga actctttttg agaactgccg
    90721 atgtgtcttc ctttcttgtc aaagataatt gctcgtgagc tagttgtacc ttggtcaatc
    90781 gccatgatat acgttgattc tgtcattgtg attccctcca ttatatgcaa acgctttctt
    90841 taactactct tatcatacta ctgttttaaa aaaagaaact gtcaaaattt tattcttttt
    90901 ttcttttttt gacagttttt tattatgaag ctatttttta atcaattctt ttcttttttc
    90961 agcgtctcta ttcttctttt caatcgaata atgtcgtaag gtgacgcaaa ttctgacaga
    91021 taatattcac actgtttttt gtttctctgg caaaatgtga tcaccaaatc atattcttta
    91081 ccttcttcat aacttccaat ctcaataccg ccgattcccc tcagttctct gatcagatat
    91141 tgttgaaaag caatacgata aatcggcaga ctttctaaat cgattccaat cactactggt
    91201 tttgcaatat aaaaatcgat catcaaaaga acattggcat actggatcat caggtaacta
    91261 agctcactat cttcagctct tttcctcggc aattgctgca ccgctgtttc ctgtaatttc
    91321 gccagtaact gtgttaagga atgcccgagc attttctgct gctgttccaa aagccgatca
    91381 cgctcataga cttcgatctt tccaacaaag aaaaaccatt cattgttgat ctgggaaatt
    91441 tggtagccta ctgccttttc ttctttgatt ttcaaacgac gaggacgata atgccataac
    91501 atcgtttccc gaatgtaagt atctaaaaca gcggttggta actttttgct ccgtgtcaaa
    91561 tcatactgat aaaatatttc ttcgtctact atcgaaaaag aaacaaaaaa gctgtaaaac
    91621 atcattgttt cgtatacatt cagttctgct gctgttcgac taagatgcat cacgatgatt
    91681 ggatcgattg cttgataaag ccggtccgac tgatagagta atttcttttc tttcaaagtt
    91741 gcgtacgtac ttttttcatt caatagtctt tttttcgtaa tcccgagcca gcaagcaatc
    91801 tgtgcttctg ctgatgccgt aaacgttgtt tctaatacac ggtccaatcc tagaatgaac
    91861 ggtctttttt ctggtgtatt ttgcaaaaaa agcggtcgct gatcttccgg gatatattgg
    91921 aatagttcat aataaaaata tcgtatctgt agctcttctc cgtataattg accgtttttg
    91981 atttgtaatc caaattcctc taagagctgg ttcaattctt tgatctttcg aaaaacagaa
    92041 gactcactga tattaaacgc tgtcgctaat cgaacgatgg atacttgctg atgttctaat
    92101 aaatagacga gcatcttaaa cttcaagctt tcctgcaata aaaacaatag aattttttcc
    92161 aaacttacat cttcgtccaa cacaagcagc gcctgatact catttcgctg tacttctact
    92221 tttttttcca tcattcggcc aatcaattca atatcttcca aatattgatc aaacgaggct
    92281 tttgacagtt ggacatgctc tctcaaacca ttgatagagg ctgttccccc ttgcagaaca
    92341 aggtttcgca aaatcaggat ctgtaactgc tcttttcttt ctaataatgc ttcaatcttc
    92401 ataggcagta ttcgccacct ttgaccacac catatctggc acacggtatt ctggctggaa
    92461 aaatagctca tgttcttcta actgttcaaa cgtccatcct tcttctactg caattgtcag
    92521 ttggtcaaga aggtggaaca tctctttttt gctttggagc tgtgctccaa gaatcttttt
    92581 ttcagaatca aatagaaatt tgagtttaac tttgctttga tttattgctg taagcttgta
    92641 gtctacgtat cctgtttcaa ttttttgggg ataaaaaatg ctctcttctt ctgtcaaacc
    92701 tacgcttcct aagaaatagc caaaccactg attgccgact ggacgatagg ttccctgatc
    92761 ctttgtctgt gagccactaa ttgtcttgct gacatttcgt gctgtacgga cagcgttatt
    92821 tactagagaa acatacattt tttcatttgt tggacgaaaa gttacttgga tcgcatcgcc
    92881 aatcgcataa acgtcttttt gagacgtttg caagtagtca tctacccaga tcgttccatc
    92941 atcattcaat gttaattgtt cttcccacat ctgattgtcg gggcgagaat gattagccag
    93001 caaaacaaga tcatagttct cctttctttt ttccgtctct acaatagcgg tttcattttc
    93061 ggtcaaagac acaacttgtt cttctaaaaa aagtgaaagc ccttgatttt tcatttcctt
    93121 tactaacggt tctgtcattt caggatctag ataacgaaaa agaattgact ttcggctttc
    93181 gtaaagatgg atttgttttt ttcttgatgc caacccttca gctatttcga ttcctgcttg
    93241 tcctgcacca ataattgcaa ttttctttgc attcatgatc ttttgctgaa gagccgttac
    93301 gttttgtatc tttttaactg ggtgaataag cgttgattct gcagaaatat ttcgaaactt
    93361 ttgatttgaa ccagtcgcta gaatcaaacg atcaaaatcc aatttttttc ctcctgctaa
    93421 aacgactttt ttttctttca atcctattac tgtcttttct gtgtaaacat ggattcgata
    93481 gcggtcagtc agttcttctt tcgtgatcca agaaagttgt ttttcactgg taacctttcc
    93541 ttttaatatc aaattgaacc cgctgggaat ccatcctaac ttcgattgct tttcaatcaa
    93601 atatatttca cttgttggca tcaattggcg aagataaata gccgcatgaa tccctgcaaa
    93661 cgacccgccg acaattacaa tgttcatctg cattcctctt tcttataagg tgttctactg
    93721 ctaacttctc aatcagaaaa cacagtattc ataactcgct ttcaacctta tgatacatag
    93781 cgatgcagca gaatcaaccc tctccatcat accgtgttct aggtctgctt ctcaataatg
    93841 tactagtgga cctaattgaa gagtatcatt tctgaagaga tgtttcaaaa aatgtactct
    93901 tataaaagaa aaaaactcgc cttttacgta cctctgtcca aataggaaac agaaaagaac
    93961 gaaaagacga gttttagcgt gtgtaatgta atagttagaa tagacacatc atttattttt
    94021 aattctttaa tgcttcaaca actgcttcgc ctaattgatg agcagattga ggattttgcc
    94081 ctgtaagaag ccgcccatca atttgaacca cttctgaaaa agctgctcca gactgatagt
    94141 ttgctccttt ttctttcaaa gcatcttctg ctaagaatgg tacttcattc gttgtaccat
    94201 ttgcttcctc ttcttcatta ctgaacccag tcactgtacg tccagctaaa atagattttc
    94261 cagactcatc tgtaactggc aacaatccta caacaccgtg acaaacagct gagaccagcc
    94321 cttcatttcg atagatttgt gaagcgactt tcccaagatc tattgcttct ggaaaatccc
    94381 acattgttcc gtggccacca gcataataaa ccgctgcata atcatttgca tccacatcat
    94441 caggacgtaa ggtatttccc aaggcccgat ttctgaaatc ttcatcttca taataggccc
    94501 aatcgatttc atccatattc aaaacacttt gcggttctaa aggtacatat ccgcctttag
    94561 gactgacaaa atctgcatcg atcccatttt ttactaatac atcataaaaa tgagtcagtt
    94621 cacttaacca caaacctgta ggacgctcga tcttttcgta cttttctaca tttgttaata
    94681 cgatcaatac tttcattttc ccatcctctt tctataaaaa ttgcgttatt gataacgtgc
    94741 tttcaagaaa ttatataatg ggcttggcaa ataaagctct ttatatgctt cttcaaaagg
    94801 tttcgttaca aattcatcct cacggttttc caataatttc tctgcccagt caggatcgac
    94861 gatcatttgc tgtccaattg ctgcaagttc tgcatatttc aatacctgct ctacatcttc
    94921 gcgattgcgg atcccaccga caccaactaa agggatcttt ccttgaatcg tgttatggac
    94981 cacttcaaga atcgatttat cgttgtattt atcagaacga gctattcgat catacatatt
    95041 taatgaaaca tgcagataat ctagttttga ctcacgtaat cgttcaagaa gccagatcgt
    95101 gtcttcaaaa cggatacctg gtgtttcata ttcttctggt gagaaacgat accctacgat
    95161 gaatggtttt ttagcatatt gttcaaccgc tgagaaaacc gttgttaata attcttcaat
    95221 aaaacgataa cgattttcca agctaccgcc ccattgatcc tctcgtctat tcgaatgcgg
    95281 agagaaaaat tgttggatca aatacgtatt tgctccatgc aactcgattc catcaaatcc
    95341 tgattcaatc gctctacgtg tggctttgcc aaatgcttgg attgtttcaa cgatttcttc
    95401 ttcactcatt gctcgtggtg tttccgcatc aggacgtttt gctgcaactg cacttgcaga
    95461 tacaggctgt tctccagata aagttgctcg acttgtcatt cgtccaccgt ggaaaatctg
    95521 aataatcgct tttgcgcctt ctccttggat tgctttcgct agctcactta atcgaggaat
    95581 catttcatca tgagcgatgc tgagttcacc tggccagcct tttccgatat cttggacatt
    95641 tgcagcacca gtaataacag caccaagacc tttagaacgt tgtttataat aagtgatctc
    95701 atcttcggtg atcactccat tgaaaaagct gagttgtgtc gtcataggcg acagcatcaa
    95761 tcggttcttc aattgaactc ctgaaggtaa agtaattgat tgatcaaatt gtgacattgt
    95821 cattcctccc ataattcgct ctgattaacc tatcgagaat catctatcat taaaccactc
    95881 gattagttgt acatgcaact aatttgctta aaaaatttta ttttttttgt aacttatctg
    95941 ttgcatcatt cattgtctgg gctaattctt tacttgcttc atttcccaaa actgctttgt
    96001 atgactgatg aagttcttcc cagctaccat cgacttcctt agcaaattgg cgtccttgtt
    96061 ctgtaagata aactaatgac aagcgacctt cgctaactgt tttgacgtat cctttatatt
    96121 ctaatttttc aacaaaacga gcaatcgttg atggggcgga atagagcatt tgtgcaatat
    96181 ctttttgtgg tattccatct ttttctttta atatcaataa aataaaacca taagttggtg
    96241 ccagacctgt ccttctaaac gcttcatcag ctaatttatt caacatgcga tctagctttt
    96301 tcacgctaaa gaaaagacat tcatctaacg ctttttcaat tggcttttcc atttcatccc
    96361 ctccattggc gtacatatca tagtatgatt tttatttttt gtcaaaaaac acggaataat
    96421 gtccgcctcc tctgtctaga tttgaaaaaa agaagtagcc atcttcaaag aatttcggct
    96481 tgttttcgac aaaactcctt ctggaacacc gtgaatcaga ggttgtgaaa aagtcgttta
    96541 gttttgagta tgaagaaaaa ttttgtgaaa atggctttcc acatttttgc ataaatttgg
    96601 cttaataccg aaaaactggc ttttgaacac cgtttatatc agaggttgtg aaaaagccgt
    96661 cctgcatcaa gtaataagaa cagccaaaca aaaatagttc ctcatatttt gtcgtttttt
    96721 gagctaatgc cgatggtcag ctgattgtac accgtttatt gagaggtcgt gagcctgccg
    96781 ttgaactccg agtcacaagg agcaatttca aaaaacagct tctcatgttt tatgactatg
    96841 gtgacttgtg atcgaggagt tggcaggaga atactgttta ttcgtaaaga aggggctgtg
    96901 acaagacttt tgtcacagtc ccttatttcc ttttattata ggatttcgcc atttgattgg
    96961 atgacttgtc tgtaccaatc aaatgatttt ttctttaacc gtttctttgt gccattccct
    97021 tcgtcatctt gatcaacata gatatatcca taacgttttg acatttgtga agtagaagcg
    97081 ctgacgatat caatgcagcc ccacgacgta taaccaaata aatctactcc atctttgata
    97141 gcttccttca tctgctcaat atgctgtttc aaatagtcaa tgcggtaatc gtcttgaacg
    97201 gttccatctt cttctacttt gtctatagct ccgagtccgt tttcagctat gaacagtggt
    97261 ttttgatacc tatcatataa ttgattcaaa gcaattctca aacccttagg atcaatctgc
    97321 cagccccatt cacttgatgg aagatacgga tttttaattc ctccagttat taagtttcct
    97381 tggacccctt cccaattttc tggatgagca ctgactgcac tagacatgta gtagctaaaa
    97441 gaaacaaaat caacagggta ggctgctaag atttcttcat cacctacttc tatttttaag
    97501 ttgacacctt tttcttcaaa cattcgttgc gtatacgctg gataatatcc tcttgcttga
    97561 acatcactaa agaacaaaga agttctttgc tgctcaattg ttttttgaat atcatccgga
    97621 tcacaagaat acggaacaag aagctgatag ctgatcatac aaccgacttg tgaacctttg
    97681 ataatttcat gacaagcttt tgtagctaaa gcacttgcta taaactgatg gtgtgcacct
    97741 tggtatttcg cttgttccaa atggggatgg ttttcttcaa ttactcctac gctaacaaaa
    97801 gggtgatgtt ttacacagtt gatctcattg aaagtcatcc aatattttac tttattttta
    97861 tagcgtttga atactgtttc agcgaaacga acgtaaaact gaataagctt tcgattaggc
    97921 catccaccat attctgtaac caaatataac ggcatttcat aatgggaaag agaaacgact
    97981 ggctcaatcc catattttag acattcgtca aacacttgat cataaaaaaa taatcccgct
    98041 tcattgggtg tttcttcatc accatgagga aaaattcttg tccatgcgat agacatccga
    98101 tagcatttaa aacccatttc agcaaataaa cggatgtctt ctttaaagtg atgatagaaa
    98161 tcatttccgt gtcttttagg ataataatac ttgctgttgg gatcctgcgc cgcttttact
    98221 tgctgatgcg tcatcatatg ccattgatct gtccattttt ctttaggtaa atcgctgtcg
    98281 aatgtataca catcagacac agataatcct ttttcatcta catcccatgc accttcacat
    98341 tgattggccg ctgtcgctcc gccccataaa aatccttctg gaaatttttt catatcgatc
    98401 cccctaaact tgctagtttt cttgtttgat tatttttctt atgactagta atgatatttt
    98461 tgaatctact actatttgta acaataacca ttgtggtcgg atcatatccc tctttgatta
    98521 attcttgcaa atctacttcc atcaataaat cgcctttacg tacttttttt ccatcagata
    98581 ctgcactttg aaagtatttg ccatttaaat tcactgtatc aataccgata tggatcagta
    98641 tctctgctcc ttcttcactg actaccccaa ttgcatgttt cgttggaaaa actgttgaaa
    98701 cttccccgtt tacaggactg tagatttttc cgtccgttgg aatgatcgca aacccttctc
    98761 ctaaagatcc actagcaaat gttggatcgt ttacttcaga taaatcaatt atttcacctg
    98821 ctgcaggagt acttaacact atatcctttg aggattcttc ttcgatttca tccttgtagc
    98881 cgaaaagaaa agttagcata caggaactga tcatcgctaa gaaataacta gcaactagcc
    98941 aaacaaatcc ttcaccaata taaatcggga aagacaaaat acttcttgta gcaactgcca
    99001 aagcaccggc tcctgcggca ccagctactc caccaaatat agctccacat atacaagcaa
    99061 tgtaaaatgg tcgtttatat ttaagagtca caccataaac agcaggttca gtgattccaa
    99121 ataatgctgt gataaatgca gataaagata aactttttac tttcttattt ttcgttttca
    99181 aaaatacccc aagtactgct cctgcttgcg cagctacggc agtcatacat gctggaccta
    99241 gcgtatcacg accgtagaaa gaaagattgt tggtcattac tggtacaagt ccccaatgta
    99301 acccaaaaat cactaacact tgccaaaatc cgccgataac agcaccagat aaaacagggc
    99361 taaagttata aacagctaaa tatccagctg caatcagctg acttgcataa tctgcaattg
    99421 gacctacaac taataaagta attgggacca tcacgatgat acaaatgaat ggtgtcaata
    99481 aatttcgaat actttcgtgc aagcttttgc gaatcttagg ctcaatataa gaaagaaccc
    99541 atacagctaa aattgctgga ataaccgtac ttgtatacct tgctagaatc acgggcatcc
    99601 ctagaaatcg caaggttatg ctatcactat atgcactgac aatcgtagga taaacgagag
    99661 ctgctgcaat aaccatagcg atgaaacgat ctgtattgaa cttttttgca gcagtatatg
    99721 ccaaaaatat aggcaagaaa tagaaaaatg catcagctgc tgcattcaaa atgatataag
    99781 ttcctgattc agtagttaat aaattcaaac tcgtacataa ggcgagtact cctttgatca
    99841 aaccacttcc tgccaagacg cctaaagtcg gggcaaaaac agcggcgatc atgtctataa
    99901 aagagtttaa aatgcctttt ttctcagttc ctctctttcc actagatgca tcatcttcta
    99961 gtttcatttg tttggataat acgtcataca cttcatttac tgcatttcct actacgactt
   100021 gaaacattcc tccagcttcg actacagtga tcacaccttc tagttgctct accttctcct
   100081 tatctgcctt cttattatct tttagggtaa agcgtaacct tgttgcacaa tggtaaacat
   100141 tgctgacatt ttcttcgcct ccgacaaatc ggacaatatc tttcgctaat tgtgaatagt
   100201 ccatttttac ttcccccttg ttaattaatg ccgtagcaat ccttcttaca agataagtat
   100261 agttcttaga aaacggtttc tccattcaat tagtacccct tataatagcg aacgaaactg
   100321 tacaatagtg tgtatggttt acttagccga gcaaatcact cactgcttga aagaaaacaa
   100381 gtatatcgtt tctcttatag tcaaaggaga caaaaaaaac acgcttgatc ttcctttgct
   100441 gatgtataag tggtatctca agctgatact ctgtgatttt tgtttctact tggcttttga
   100501 taagacagca caacaaagtc tcggcattta acggaagcat aactaggtca gaactaggag
   100561 agattagttg ctgaatggaa tctatctttt tatggatttt tatttgattt tcttctttgt
   100621 agtaatgatc aagcagtttt gttttctttt tttcttcaac tatggtcagc ttttcacgca
   100681 aaatctgata aatctcccaa gattcaaact catctagaac actaggtaga taaatcaaat
   100741 catcccactc aaaaataagc tcttgctcag tagtcaaaat tgcatcatat tccgttattt
   100801 tgtttgattg atttttgaaa agatacactg gttctatccg gcattctttg atccacatgt
   100861 tcatactttg ttgaatctgt ttttttagtg tattcaaaat actaattggc tggttactaa
   100921 caatcagtat ctccccttgt tttcgaaaag gattcaacgc ttcggcaaga tacaaaacaa
   100981 aataagcgct ttcagctaaa ttgggacgta attgaaaatg tattgggaag aaacgccgaa
   101041 ttaaataaat ttctaaagga taacgggaga tgatcttttg tacaaaatga ttcatggtat
   101101 tatgtccggc atccatcctc gttttcattt gtttgatatg ggttagcaaa aattctgttt
   101161 catggaacaa taacgaaaca ggaagatcta aaaaacgaat aatcgtttgc tcaaatgctc
   101221 ttagattctt ctctatttct ttgttttttt catcattaat catcaaataa tttaactcta
   101281 atagtcgatt ttcaattgct tgtttttctt gattggaaaa agaatagggt agttctgatg
   101341 ataacttttt tgtgagggtc tctaccatcg gaataaataa tgtatacctg tttgtttttt
   101401 ctaaaagttc ttctcttcga agaatggtca aaacgagata ccgagaaaat cgtatgaagt
   101461 cctctcctga aacaatataa ttatatgata taagaacctc ttttaatgtc ttttggacag
   101521 tcggcatgat attggaaaaa attgtactga tttgttgagg caaccttgcg tgttggatca
   101581 ccgtttctgt tccaacaaga gataagaaaa ttctttttga attttcttca gcaatgattt
   101641 ttagtccttt tgtcgacgaa acttccagtt ctaatttagg attgcgctcg atccaacgca
   101701 aaatcgtttt tatttgtaat gatacggctg tttttgaaag aaagaaaatg tctgctatct
   101761 tttgcatact gacataccct ttttgaaaca ataagtacgt cacaatagtt gaagctcgga
   101821 aatccatgct ggaatagatt ttatgtttcg tcacctcttg tatgatttgc tgtttagttt
   101881 cagattcaat tgattctaaa aaataacctg aacgtttatt tgactggata atgattttct
   101941 tctcgtagat agcaagcata tttttgattt cagtttggat cgttttcttg ttggtaccta
   102001 aagcttcagc taattcttcg cttgttatcc aatgattctt atttcttaat actacaagaa
   102061 tttggagttg tcgtttattt aagtccattt gcttacacct cctctttgtt ataaacatac
   102121 aggaaaaacc tctcaaatga caatagtcac ttgcagaggt ttaaaaatca tttgaagaaa
   102181 gatctatatt caaggttttt accattagac tggataattt gtttataata ttcaaaacta
   102241 tcttttttca atctcctaaa gcttccgctc ccatcatttt gtctatccac ataaataaag
   102301 ccatatcgct tcctcatttc ccctgttcct gccgatacga tatcgattgg tccccaccat
   102361 aagtaaccaa tgatttggca accatctttt attgcctcag caatttcctg aagatgtatt
   102421 tttagatact cacaacgttt tggatcgtgg attcgattca attcatctgg tgcttcatct
   102481 agtccaatgc cattttcaac tatgaataat ggtaattcat aacggtctgt caattcattc
   102541 atgacataac gaaaccctag aggatcaatt ggccaactcc aaggttcggg agatttttcc
   102601 ttcaaaaagg gattatcgaa accatttgct ccgccagtat ctacgcgcat ttctgcttct
   102661 tttttataga cagcgctacg ataataacta aatgcaatat aatctgccgt gttagcttgc
   102721 aagcactgtt cgtctccatc taacattact ggtgcacagt cttcttcctt ccatgtcctg
   102781 taaatataag ctggataatg ccctcggcac atgacatcgg agaaaaacca tgaattgcgt
   102841 ttaaattgca gtgaaccaaa tacattctct ggatcacagt catatgcata agtcgctaat
   102901 gcagaaaagc tacacataat acccatctta gcatttggat cgattttctt taataattga
   102961 gtagcacggg cgttcgctag aaacatatga tgacaggctt gataaatcac tttttttgtt
   103021 agtgtagcaa tgggttcaag tttatctcta gcagggaatg taaccaatcc agcagctgca
   103081 taaggcatac gaaaagcatt attgatctca ttaaaggtca tccaatattt cactttccct
   103141 tggtagcgtt caaagacggt ctgcgcataa aattcaaaaa agtcgattaa ttttcgattt
   103201 tgccaccccc catactctcc taccaatgct aagggggttt cataatgact aagagtaatt
   103261 actggctcga tatcatatcg atttaatgta ttgattagtc tgtcataaaa tacgagacca
   103321 gcctcatttg gcttctgttc gtctcctctt ggaaatattc gtgcccatga gatgcttgtt
   103381 cgaaaagctt ttaaacctaa ctccttcagt aaacgaatat cctcttcaaa tcgatgataa
   103441 aaatcaatcg cttcatgaga aagataatgg agtgattcgt ctaaagcaat ctcccaaatc
   103501 tttttttctt cattccactg aatactagga tgctggttca tgatcccgat aagcgtatct
   103561 gtcacattag gtaatttgcc gtcttccaac catgcacctt cacattgatt agcagctact
   103621 gctccgcccc ataaaaaatt ttctggaaat atttcctgat ccataactat ctctcctcgc
   103681 tatcaacaat ttcttacttg aagtatagct cgattcatca aaacaagata ttctaactat
   103741 ttcggcagaa gagaagaaaa aaattgaatg ctactcttca tttttaatat ttatatataa
   103801 caaatacata agacgatccc cgaaagaaaa cactcgttaa ccatctaagc aaacgattta
   103861 ttttacttcc aacacaaatg cttcataagg tcgtaaatca actttgccgt ctggtaactg
   103921 tttcagagga taatttgaca caataaccct taaagcttca cgtggaacag tgaacgtctg
   103981 cttttcactg gacatattac aaataacgag ccattgctgg tcttcatata tgcgagaata
   104041 ggcaaagaca tgctcaggcg tcttttccac taattcatag tctccccaga cgatgatgtc
   104101 atgttttcta cgcaaatcga tcaatttctt gtacatataa aaaacagaat cttgatctgc
   104161 taacgcttct tctgcattga tttctttata atttggattg actcgaagcc atggaattcc
   104221 agtagtaaac ccagcatgtt cacgggcgtt ccattgcata ggcgtcctag cattgtctct
   104281 tcctttcgca ttgattgatt ccaagatttc ctcttttgta aatccagaag aaatcctttc
   104341 gttatacata ttgattgtct caatatcttt tgcttccgcg atatcatcga ttgggcaatt
   104401 cgtcatgcct aactcttctc cttgatagat ataaggggtg cctttcatca tatggagcaa
   104461 gatagcaaac atcttggcac tttcttttcg atacacatcg tcatttcccc aacgagaaac
   104521 aattcgcgga agatcgtgat tattccaaaa taaactattc catccttcat cacctaaaga
   104581 tgtttgccat ttggataata cttttttcag ttcagagata ctcaagggtt tcaaatccca
   104641 tttgtctttg ccttcttgct gatctaatcc tatgtgttcg aattggaaga ccatggataa
   104701 ttcatttctt ttaggattgg aataaagttt agcgatttcg gtagttgctc cccatgtttc
   104761 gccaacagtc atcagatttt tgtcaccaaa agttgcttga ttcatttcct gaagatactc
   104821 atgcagttta ggtccattac ttgtgatttc tttatccggt tctttgccaa tcatatcgat
   104881 cacgtccata cgaaagcccc caataccttt atcgatccag aaattcatca tttgataaat
   104941 ctcttggcga acctgtttat ttttccaatt caaatcaggt tgacgcttgc tatacaagtg
   105001 cagaaaatat tgccccgttt tttcatccaa ctgccaagca gaaccactga atattgagcg
   105061 caatctatta ggctcttttc cattgaccgg atcacgccaa atataatagt cccgataagg
   105121 attttcttta tttttacggg cctcaatgaa ccacggatgt tcatcagaag tatgattcac
   105181 caccagatcc atgatgatac ggatatttct tttctttgct tcttcaatca gttcttccat
   105241 ttcttccatc gtaccgaatt ctgccatgat tgcttgataa ttactgatat catacccatt
   105301 gtcatcattc ggagactcgt atactggaga aagccaaatt gcatcaatac ctaaatacgc
   105361 cagataatcc aacctggaaa taatcccccg caaatctcca ataccatctt gatttgaatc
   105421 ttgaaaactt cttggataga tttgataaac aactgaatgg tgccaccatt tttgttccat
   105481 gattttcctc ctataaatgg atgtttctat tttttgctta atcatttatt ttagtctctc
   105541 tcactaaaaa ttgtaacgct ttcaaatagc tattgcacct tgttatcggt aacagaataa
   105601 aacaaaaaaa cagctgagga aattccctca actgtttttt gaaaggattg atttatgcag
   105661 ggaagaatgc tgatttcaca acttctttat aaacggtgtt cactcagctg tcccttggta
   105721 ttagctcaaa aaacggcaaa atatgaggaa ctattttttc gtttttttct ctaatactca
   105781 ggccagaaca ctgatttcac aacctcttta taaacggtgt tcactcagct gtccctcggt
   105841 attagctcaa aaaacggcaa aatataagaa actatttttt cgtttttttc tctaatactc
   105901 aggccagaac gctgatttca caacctcttt atttcacatt caatatccac atgaatccca
   105961 caaatacaaa gaacagacca tacatgattg gagatacttc ttttcctctt ttagctgcta
   106021 acatggtaat tggatagaag ataaaaccta atgcaatccc atctgaaata ctataagtca
   106081 aaggcatgcc gatcaagatc aagaatgaag ggatcgcaat ctctaagttt ccccattcga
   106141 tttgtcgcaa agattgtgcc atcaatactc ctaccataat caatgctggt gcagttactt
   106201 gtgaagtaac aactgatagt aatggagaaa agaacaagcc taacaagaag aaaatccctg
   106261 tagtaatagc tgtgatacct gaacgtcctc caacggcgat accagctgat gattctacaa
   106321 atgcaccaac aggagaagta ccaaataagg aacccgcaag catagctgtt gaatcagaag
   106381 ctaaagcttt ccctacacga ggcatcttgt tgtctttcat gaaacctgct tggtttgcta
   106441 atccaactaa agttcctgct gtatcaaaga aagtcacaag caagaatgtc agcactacga
   106501 cccacatttg aagagaattg atgtccccta catgttttaa tgcaactaaa aatgttggtt
   106561 tcaaacttgg tgctgcggca acaatttcag aaggcattgg aatcaaacct gtgaccagac
   106621 ctaaaatcgt tgtagctgcc atcccgatga agatgccacc tggtacacgc cgaacaagca
   106681 agatagcagt tacgactaat ccaaaaatcg tcaaccaagt agaaccaacg ttgagtgatc
   106741 ctaatcctac taatgtagat tcattcgaaa cgatgatccc accttcgctc agaccaagga
   106801 aagcgataaa tagaccgata ccgccagaaa tcgcatattt caaatctgct gggatcgcat
   106861 caatgatcat ttcccgcagc ttgaagattg tgatcaaaat aaaaatcaaa gaagcaacga
   106921 atacgccggc taatgctgtt tcccatggaa tccccattcc taaacaaaca gaataagcaa
   106981 aaaaagcatt gatcccaaga gcaggtgccg ttccaattgg ataacgggct aataccccca
   107041 ttaaaacaca gccgatcgca ctggctaatg cagtcgcagt aaacactgct ccttcatcca
   107101 ttccagaagc acctaataca gttggattta caaataagat ataagccatt gagataaatg
   107161 ttgtaaatcc tgccagtacc tcacgtttta ccgtggtgtt caatttttct atttcgaagt
   107221 aggaaagtat tttttccttc atcttccatc cccctaaatc cgaacattat aaataaagtc
   107281 cgttaaaatt tcgatttatt aataaaacaa cgtgttttat catatactaa aacaaaacat
   107341 aagtaaacaa aaaattcgga ttttaagcaa aatccgaacg ttccatctga ttaaatccct
   107401 ttatctcaaa gttcatgaac attcactttg gattacaaga atttctttat tgtgttaata
   107461 aagtttcttc attctagtct caaaaatcaa tagtgtgatt tgattttacc atttcgtttc
   107521 tgttcttctt tgatgaattc ttttgtatcg acaggattca gattatttag tccgccaaaa
   107581 cctgtatcga ccatttttcc ccagcctttt gtcttctttt ttcctgattt gagattcttt
   107641 gctcgaagct tctcactatg ttttgctcgc cgatacttca aataaccttc taatcttccc
   107701 attagatcca ctgtttttcc tcctttttat tcaatcagac gactcagctt ttcctataac
   107761 aaaataattg ccttcagggt cagaaaaatt gaaagtcaag atgccattgt tatctgtgat
   107821 ttctcctggt tcttccatgt tctcgtagat agtttcaaat tcatccgtaa aaaatactaa
   107881 agaaggcggt ggtcctaata cctctggtga aaacttctgc acaaatactt ttgggaatac
   107941 gcctaattct aattcttttg agatgctgag tacaatactc ttaaattctt ctggcaattc
   108001 gattgtttca gaaatttctg caccaatttt ttctacaaag aatcggcaaa tcatctctac
   108061 gtcattgaca taaagcatca cacgtgtttt ttgtatcatc atttgaccct tctttctatt
   108121 tgattttctt tcctgtctat aaactgattc cattatatca cgaaagcgca aacaccttgt
   108181 gtttgatact attcaagaaa aataaagcaa tagtttctag gattgctttc ctccacaaac
   108241 tttttttaca aaaaaaaaaa cggcaatgaa tgccgctctt cttatttcga ttcttttata
   108301 attctgcttc tgattcatct ttggttagtg tgtgtctaac actagttacg ccaaatgttt
   108361 gatcgatttt ttccttcaac gcttcgattg cctgtgcgcc acgtgccgct tgttcagcat
   108421 tgatggattc aatgaccagc accgctgctt gtgtttcttc cgtcagcatt gttctttggg
   108481 cttcacgcca gttgagacag cgacagatcg ctccttgttc atcatagtag atgatttctt
   108541 ccggaagtgc cggagcatcg gaatctgcac ctaaaggcag gaatgattcg ccaccttggg
   108601 cttttcctaa atgcagattt cctgcgatct tttcgatatc ttctccgccg caaggtacgc
   108661 caaaagacaa ggaaatacta ttatagatat ctacaagcgg attgattggg aaaaattccc
   108721 gttcttgatt tgcacgtttc agtaaagctt caatagaaga acgtgcacct tttttagtct
   108781 tgaatgtggt aaatgcctga cgccactgag caatgacttc gttttggctg aatgtttctt
   108841 ctgtaaggta atttcttgct tcttttgctg cctcattcaa taattgatga tacgtttctt
   108901 cactttcttt tgttacgtgg ttatcgatac catttacgga taagatttcg atggttgctt
   108961 caggaaatac ttcccagaaa ttggtatcca ctattacttt tttcatctgt tcacacctct
   109021 tgattagaat aattgcttga tgaatagata gtctgtttgg ggatcatctc ccatataaaa
   109081 aacatgttcg ctgtatttga taaatccttt tttacgataa aaagcgagcg caacatcgtt
   109141 gtgttcccaa acaccgagcc aaatggctgt tttttgcttt tcttttgctt tttcaatagc
   109201 tgtatctagt aaaaatgtac cgataccttg atgtttaaat ttttttcgta catagattcg
   109261 ctcgatttct aaaaaatgat cagccatttc ctcagattga ctcgttcctg tatttagttt
   109321 caggtaaccc gcaggctctt gatcgatact cactaggaaa aaatcggttt ctttatcttt
   109381 tagctcttct gtaagttttt ctagtgcata agcttgtgtc aagtaatcag ctaaatgctc
   109441 cggtgaatta tcttttccaa atgtatctgt aaatgtttcg atactaattt gttgtagtaa
   109501 gtctacatct gaaggcgtca tttttcgaat ttcgtattta ttcacggctt cttcctttca
   109561 atagatcctt ttattgcctt ttttgacaaa attccaatct tcgctaacat tcttgcatac
   109621 tttatttaaa agatattcca attgttttgc ttcttcgtca gaaagaccat ttaatgcgac
   109681 tgcattggag tagttgtttt ctcgaatgat gaaaggatag atttctgctc cttttttagt
   109741 cggatagagt ttcttgattt ttttattttc tttatcttcc aaacgttcga tcatcccatt
   109801 ggattctaat ttcttgattg ctcgggcagt cgttgtacga tctactttga tcatttctgc
   109861 tagtttttct tggatgattc caggattttc gcaaatacgt actaaataaa gatactgtcc
   109921 tcttgttaaa tcgacttctt tgaattcgat attgctaata gaatctagcg ctcgggcaat
   109981 cattccgatg tctcgaagga tttcagccat ttcatcccac ctttattttg tttgtaagac
   110041 tagtttacct ctttttgttg caaatgcaac tattttcaat aaaaaaatca agttcttttt
   110101 taataactag taaagtatat tcttttcttc ttttacgaaa aagctcaatc acaatagatc
   110161 tggtttttgt tttctaaaat aagtaactgt ttcttcgttg ttcgcttttc tgatagcttt
   110221 ggcaactctt ctatactaaa aaatcccatg ttggaagttt cattattctc aataaacttg
   110281 gcctcttcac cgtggatcgc acaagcaaaa acaagtttat aatactgaaa aagctgtgga
   110341 atatcttttt gtttattcgt atcaaaaact gctcgcaact gagtagtttc tactgttaat
   110401 cctgtttctt catagacttc tttacgcaca ttttcttcag gagacaatcc tatttcagca
   110461 aatccaccag gaagtgacca ttctttcgtc cgtttatctt cgattagtag gaccttgcca
   110521 tcttttttga tataggcacg aacatctact tttggtgttg gatgtccttc tccaatcgtt
   110581 gtgagcgctt ttatttcttc gaaagtttct tttcctattt cactgatcag ttccaatgaa
   110641 atcgtccgga tctcctcaaa acgctcttta tcaaatacat cttttccata atatagacca
   110701 gcttgagaaa gtgcgagtag tcttttgtat tgagccaact tcttgttcat tctcttccct
   110761 tcttttttag caaatgtatg actatgtaat caagtataga agactggttt aattacggca
   110821 aataaatata atgaaatctg caataaataa aagaatgtta aaaaactcga gaacaaaaga
   110881 cattcgactt ctctggggct gtgaataaat ggcgttcaaa agcttgcatc tgcggtaata
   110941 agccccacca aacgaaaaat atgacgagct attcttgtct ggctgttctt attaattgag
   111001 gcaggacagc tttttcacaa cctccccact aaacggtgtt ctcctaccaa ctcctcggtc
   111061 acaagtcaca atattcatca aacatgagaa gctgtttttt tgaaattgct ccttgtgact
   111121 cggagctgaa cgacaggctc acgacctctc ggtaaacagc gttcaaaagc caatttttcg
   111181 atattaagcc atttctttgt tacttattgt cattgatttc tttttctaat ttatccaata
   111241 gtggagtgta atccacttga gtatagtctg ttacatcaaa ttcatatagt gttcctaact
   111301 ggaatttgtc atattttcgg tcggcgatga tctttttgaa tgcttctttc gtaatatgat
   111361 tggtcccttt cgttcgatct tccaatacgt caccatagtg ataatgatcc atgatatagc
   111421 tcaaatgacg atctgattca cgatcacgtt tgtaccgacg ttgacataac acttcaaagt
   111481 ctgctgttag gcgaatcgtg atcacctgat atccgtatct atctgctaat tcttggagtc
   111541 gtgctttttg cttatcacta aaaggatact cggacacaat cactttcttt cctgcttcca
   111601 tatacaaatc aagtgctttg taatagaaag gataaacttt cgtcctttct tgttccgccc
   111661 gttcttccaa attgttaaac ccgatggatt ctgcatagta ttccttgatt tcatcgagcg
   111721 tgatggcata catatctgga aatcgttccc gtagtttatt cataagatat gtttttcctg
   111781 taccaggact accagccaga agaatgaaat actttttcat ctcatttatt tcctaagtta
   111841 attggacttt ccaagttcat aaacagtcca atcttatgct tgacaatttc ttttactgct
   111901 tcatttgctg gaatcacatt atggcgaaga gacttgttga tttctgtttc actaaaggtt
   111961 tctttagcta ctttcgtcca gttgactaaa agctccgttc caacattgaa tttgcggatc
   112021 ccgttattga tcaattcagg ataatcttct tctttcacac ctgttcctcc atggataact
   112081 agggggacat caactactgc atcgatttct ttcagcaagg gaatgtttac ttctgttttt
   112141 gatttgaatt gtccatgatt cgtaccgata gcaatagcta atgcatccac gcctgtagct
   112201 tctacaaatt tcactgcgtc ttccggtctt gtatatacct tgtcattttc agcgacatgg
   112261 attccttctt ctgtaccgcc gattgttcca agttctcctt ctacggaaac accgtgcgca
   112321 tgggcatatt ctactactgc ttttgtcttc aggatatttt ctttgaaagg cagatgggac
   112381 ccatcataca tgacagaagt atatccgctg tcgattgctt ctttgatatc atcaaagtct
   112441 cgggcgtggt ctaagtgcaa gactgcatca accatttctt cttctgcaat agctgtcatt
   112501 acattgacta acacatgcat gccgatatat ttagcggtat cgacacttgt ttgaataatg
   112561 atcggcgaac ccatttcttt tgctgcgcct agcatagcag gaagcatttc taggttatgc
   112621 gtattaaaag ctccaactgt aaaattcaac tcttctgctg tttttgttac ttctttcaat
   112681 gttgtataca tcttttattg ccaccttatc tttttatttg tttcctttga tcgttacttg
   112741 ttttgcaatt tgtcgcatct catccatttt gcccatgtag taatcgatcc cttgatcatc
   112801 accagcacga gcagcatctg cacgcttctg gttatagagg ctcatcagtt ttttataccc
   112861 atctccgatc gtctgtttat tttccaaact cttttctaaa tattggatgg cttgatcgtt
   112921 cacatacaat tcagaaaatt taagtccaac ttgttcataa agtttcgcta atttatgctt
   112981 atcttgggta gtggaaatct cttgtttcaa ttgtgtaatc tctgattgaa tcttttcggt
   113041 cgcttctggc gatagggctt tttcttcttt tgccagaact tttttcggtt ctttttcttt
   113101 tttcttaaaa aatccaaaca tcattttccc tactttcaat taaaatgctt tcaaaactgg
   113161 gcttaagatc caggcatata agagtgctgc ggctactgta tctacatcgg ttgctgtagc
   113221 attgatgaat cccatcgaat taaagatcgt tactagcaaa gcgggcaata atgtaataaa
   113281 gaaaccatga gcaataccgc caatgatcgc tcctcggcgt ccgccaactg cgttgccaaa
   113341 tatccctgct gtccctccgg caaagaaatt ggttagcata ccaggtaaaa tcatcgctaa
   113401 gccgaacatg ggcaaagtaa acatggcaat aatcgtacca atcgttgtcg taatgaaacc
   113461 taaaatgaca gcatttgggc tataaggaaa gaaaactgga caatctaagg cagggatcgc
   113521 atcaggaact aatttcatag ctatccctcg aaatgccggc acgatttcac ctaacagtag
   113581 acgtacacca gctagcaata catacacacc cactacgaat tggatggctt gtaaaaaggc
   113641 aaacatgata tagttttgat ctcctgataa ttcagaagcg aatggttcgc ctgcaaataa
   113701 aacagtgatg atatatagcg ggaccatcac gaccatgaca gacaaataag tatcttgcag
   113761 aaattcgaaa tgtgtcggca acttgatatc ttcgatcgac tttttatttt cgcctttctc
   113821 accaaaaagc ttagcaacac cggcttcaaa taagtacccg atcgtacaaa aatgacctaa
   113881 agcaatatca tttgatccag tgattttacg aatgatcggt tgcgctactg ccggcatagc
   113941 aatggcgaaa caagctccga taaaaccacc gaccaagatc aatacgatcc ctcgcagacc
   114001 ggcaaaatag ccaaagactg tcgtcatcgt tgccatccat aaaattgctt gaccagttaa
   114061 aaaaatgtac ttccattttg tgaaacgagc caaaataata ttgaatacaa aaatggctaa
   114121 gaacgtcagt gcaatatctc tacctaaacc caaatcattc attgcttgac cattgataga
   114181 ttcaatagaa gggatgatcc cttgcatatg aaatccttca gtaaagattt tcccgaaata
   114241 agttaaactt ccaacgatga tgctcgaacc ggcactcaac acttgaaatc ctaggagtgt
   114301 ctttaacgtg ccagagatga cttgtcctgc tgatttcttc tgcagtagta gacccaacat
   114361 agcaatcaat gcaatggtga tcgatgcctg agtcaaaata ttttcgatga taaaattgat
   114421 aacgcccatg tttttaccct cttttctttt tgtatttaga taactccctt atcttgcaga
   114481 actggggtga ttttttcttc aatttcattt tttgaaacaa tatttttcaa atagatcatc
   114541 gccgttttag gatcaactgc gaacttttcg aactgcgaac gaaaattttc tgctgtgatg
   114601 atcacatctg gtttcatgga aactgcggaa gaaatatcac aatggtccag tttggcttct
   114661 actcctaatt tgttcaaaac atcctcagca gacatctgtg ctgcaaaact acttcctaaa
   114721 cctgcaccgc atacgaataa aatgttcaat ttgttcatct ttgttctccc ccgtatcatt
   114781 tattatttct caactactga aaaaagcagt tgtttgaaat cttcgatatt tgtgcattca
   114841 atcaatcgat ccaacttctc ttcgttgttg atcaattcaa ttagttcttt catgatgttc
   114901 agatgcgagt aggagtctac tgctgccaaa caaaaaataa tctttactgg atccatttca
   114961 ggattaccga aatcgattgg ttgctcaatc gtagctacgc tgactcctaa tttattgacc
   115021 ccatcttctg gtcgggcatg ggctagtgcc atgtgtttgc caatcacgat atacggtccg
   115081 taatcattca ctgcacgaat catcgcttcg acatagcgct cttcgacaat gttttcttta
   115141 accaatggtc tagcagctag ttcaatcgat tcctcccaag tagtggcttt ttctttgatc
   115201 atgatattgc tgtccgtcaa aatatctttt aacataggct ggatctccct tttgttgatt
   115261 tctaaattgt ttgtttcgaa taatttttct aacttgccat agatgattcg atctactcgg
   115321 ccaccacttt ctttcatgac atctattatt tgagtgaata actccgtcga ttcatgactg
   115381 ttaggctcta accgctgtag ttcttcattt cttttcaaaa agtcttctat aaccaagata
   115441 ttcgtctcag ttaaaatcgg atcgacaact aaaagcggct tatttcgata attcaattga
   115501 aaggtgctga aaaccagatt gacatccaat ttatctacta attcgatttc ttttgaactt
   115561 aaaactgcag taatctcgat ctgaggaaac ttttctttca gattctctgc taacagattt
   115621 ccagtagcca tcccatggtt acagacaacg actgatttat aaacataatt caagtctcgc
   115681 ttgattacac tggcgatcgt cgaaaagtgg atgacaagaa aagctatctc gtcctccagt
   115741 atcttgccac caattacttc ttcgatcgta ggaacaaaat gagtgactgc gcggtagatt
   115801 gggaaataat ttgttcggat gttttctttc aatggattca tgacttggat atgattcttg
   115861 attcttacaa tcagtgcagc catgtgctta tagagatttt cgaataaagc ttcttccttt
   115921 agatggaaag gtacgtgtgt ctcgttctct acaaattgga tcaattggat cgttagtaat
   115981 tgagcttgta cccactcgat tgaattggag atatctctag cattgaacgt ctcaatagtg
   116041 aaacgaagat aatttctttc tacttgcggg acattgtcca attgaaattc ctcgatcatg
   116101 tggtcaataa atgcagaaaa gctactgtct tttatttctg tatttttcca attaatggca
   116161 gagataaatt catgtttcct gattcgctgg atccagatca atgtaaaaag caggatttgt
   116221 tttttataaa tatcatcttc ttgtttcaac aacttattgg aatagatctc ctgtattttt
   116281 ttgatttcca ccaaaggaaa ataatggaga aagatttttt gagccggtgt taattgtgca
   116341 tcttgattca taaagaagtc ggtcattccc agattctgat tgataaaatc atagatcatc
   116401 gtacggatag atcgttctga acctttataa accagtcctt gctttccata actgacgatc
   116461 tctatcccat aactgaccag tgttgctcgt aaccgtctca tatcttcatc taatgtactt
   116521 ttagagattt gaaattcttc ttcttttttg tttagatgga tcgggattcg gctgtaagca
   116581 acggctaaaa ctagatcaaa aatacgttcg cgccgattca aaaagccgtc tttcacatct
   116641 tttatttcag tttctaatcg agcttgcttt tccttttcca gcttcaacaa aaaaactttg
   116701 ttgcgaattg tacggatctc cggaagcttt ttactgctca accagatatt cgcttcaaga
   116761 acttcctgac ggatcgtccg ctgactgaca ttgaactcac ttgctagatg ttttgctttt
   116821 actggatatt gggctgtcat gattctgtga agtatttcaa ttgttcttgt tttcatagaa
   116881 atgatctcct tctgatttca agtatatggt tccgcttaca taataaaaag tagatatttc
   116941 tgccacttga agtggaaaga aagtaagcaa agcaaacaaa aaataagcca tatgcttttt
   117001 catctcctca acgaaaaaga catacgccta ttattacaaa aattttgccc atctatacaa
   117061 aaaagaggtt gtgaaaaagc tgtcctgcat caagtaataa gaacaactaa actaaaatag
   117121 tgcttcctat ttttcgtttg gttgttctta ttatcgcaga tgcaagcttt taaacaccat
   117181 ttagtggaga ggttgtgaca gagatgttca gccttgagcg ataagaagcg attttgaaaa
   117241 atcgcttccc atattttaca aaatcgtgcc ttattgccga taggctatct ctggcacact
   117301 gtttattcag aaaaaggagc tgcgacaagg cttttgtcac aactccgttc ataaatattt
   117361 cctgtcaatc cagcataatc ggctgaataa aatcggcttg ccatcttttt tctttctcag
   117421 aaaaatcaaa ataccatatc tgagaaaaat actgtcgttg tgtcttactt cctgagatca
   117481 atcgcccatc ttttcgatcg actgaataat cacagcatct gaaatcaatt cggatggcaa
   117541 aactattttc gtggatctgt cgataatttc ctacttcctg aatagttatt tttttagtat
   117601 gatttcttac accgtctgct ttgttttttt gcagaacttt ctgatgttcg gcaaacagct
   117661 tgtctgtata acagcttctg gctttttcag gagtttcttc ttcccaagcg gattggatct
   117721 tcatgaatat cttttggatt tccagaaact gtgctttctt ttctttagcc gttcctgaca
   117781 tgcgggacaa tatctccgct ttcagccttt tcttttgtct tttattcttg acgatagaaa
   117841 aagcagaaac ccctcctact gacaaaacag ctaatgcaag aaatcccgtt tgctcataac
   117901 tagaaggacc tgtacctgga ccataatacc cataagaatc tctgccgtaa gaacgactgc
   117961 caaaataact gcccccatat gaacgaccac caaatgaacc tctgcccgaa cttcgaccgc
   118021 tagatgtacc tccaccatga ccacccgcga cagcgaatcc ttgggtcggc agagcaaaca
   118081 aacataggcc cactaacaaa atgattgcta aataattgat ttttttcatt gtcttcctcg
   118141 ttatctttta ttacaaaaaa ctatttttat gatagccttg ctaaaaggaa gagtcaataa
   118201 ccagatgcat aaactgtttt tttagcaatt ttgtcttatt tagtcattga gtaacatcgg
   118261 acaaatgtat gtgtcggcag tccataattc ctctatcttc agcatgaaca agcttgcaga
   118321 caagagatct gcgcttcctc caggagagat ttggttttcc tgacaaaagt gcaacagtgc
   118381 tgtaaatgga cgatttggta aagcactttg actagcagca tgagcaagtt tttgcatttc
   118441 cactaacgtt ttctgatcac tgcgatgcaa tacgcatgtg tcatctagcg aactcatcaa
   118501 aactaacaac atatgcattt gtttctgtac atatgaatca ttatctagct ccttagctgc
   118561 ttggattgca agcctgatat gcggataccc taatatcgct tcttcatatg caccatttac
   118621 gccatatttt tgcttcaccc tatgaccatg agtggttttt ttatgacgat attttgtatc
   118681 aggaaaagca gcaagcttgc tgacatcatc aagtattttc gataaagaaa aaggctgttc
   118741 catactgatt ttttgtgcaa tgacactgat caatagcccc attgcccaaa tcgctccttt
   118801 atgggtattg atgccatttg tagcaatcag cattgccttt tccgcttttc gcccaatctc
   118861 agcaattctt tctcttaact cctgatcaat agcatgatgc catgctattt gagccatttc
   118921 ttcaaaaaaa ggagttagcg tcttagcaga cttaagaaat aaagcatagt ccatatcgga
   118981 atgtgcaccc gcatccagtg catccactaa tccaggtttt ggacttaatg ttacttctgc
   119041 gatcaatgct tcctctacca acgcagcgag ttgctgagtc actgcttttt ttcgttcgtc
   119101 atttatcatg acactcactc cttcttccta ctattacttg ctgaaacctt ttttgatagc
   119161 tacccaaatg atatgaagta accagcttaa agcaaatgca caacttgccg tcattagata
   119221 agctacaaga atcatgactc ctgaaggtaa ctgcagccaa ctatctttaa acaataataa
   119281 cagtacttgg aaaacaaata cattggctaa atacgtacga taagcataaa cagacaacca
   119341 tttgaacaat ggcaattttg gcgaatcgtt tacaatcatt ttggaagcgc ctatgaagac
   119401 agcaaagatg atgactaaac tgtataaact ttggatcgta ttcaagtaag gtgcattcgc
   119461 aaagctcaag tctcctggat gtgcgagcaa tgaacggaca gaaaagaacg cgataactaa
   119521 ggcaacaggt aagaaaagat aacgtactct gtccaaatat tgatagactt tttcgtgata
   119581 aacaaatgcg gctaccccat aaatcatgaa actagcaaca aaccgatcta ataagtacca
   119641 gctgtcatga taaggcccag tcaatacttg tgtttgatag aacagatacc atgcgacata
   119701 taaaatcgtt gaaccaatca atactggcca tacatatttt ttattagcaa atactttgta
   119761 accgagccaa acaaaaaacg gcatgaacaa ttggatctgc agcatcataa cggtatacca
   119821 taaatgaggc gctccatcac ctgtgacata tttcagtaaa aagcttgcag tatccgtata
   119881 cggagtttgt tgttggactg ctggaaatag ccaaagataa gccgtcgtcc atagaagata
   119941 cggaacgact agttcaaaaa acttttcttt caaaaattcc caataagaca aatgctgact
   120001 tgtttttacc atattataaa caattgcaaa aataaacatt ggtgctgtgt atttaacgat
   120061 gacgtaaaag gtaccaagaa aagctgtatt tgttgcgctg tggctgtttg ccaaagcaaa
   120121 gctcaatatc gtttgcatca taacagccgt tacagctgat actttctgga agtcacttac
   120181 gttgattttg atttttttgt tttcaaacat ctctttctct ctctttttct ttattaccaa
   120241 agttctttgg ttaatttagg tccttcgact gttttcaata gaattttttt ctctggctct
   120301 ctcaaatact cttttagtga aaaaccgcct tgccgagtca ctacttgggt atcgaattgc
   120361 tcaaaaaaag caggatgcga ttgaattacc tgaattggca attcaatcgg tgaatttgcg
   120421 tacagcagca aatcaaaatc actggtttct tttactgccg gaatcccagt cgataattca
   120481 aatcctaagc tgccgccgat tccccattta catcctgata atatgtttct agcagcatga
   120541 aattgctgat aaacaggata atccatgatt ttctcttgtc tatacgattc ttgttcgatg
   120601 atgtcccacg gatgaatgac ttgtttgacg gcatgttttg agatttctgt tgaccatctc
   120661 tgattgcggg caggtccacg taaaccaatc ggtagtattt ctgcgtgatc cgtcgacgca
   120721 cgacgaactg ttccgaacat cttttcagtt agaacgaccc attcaggcaa ggtgaaccct
   120781 tctatttcat gtttatcaaa aaggacaata tcatgtgctt gaatcatttt ttatgcatcc
   120841 cattcttcgt ccattttggc acggacttta tttgtttcga cacgtccgaa tgttttggct
   120901 gtttcgtttg tgtatcgatt tgtcagcatc gtgtcatttg tattgcggac atcttgaatg
   120961 gcttcattga ttgcttcgat gactttgctg acattttgtt ctgaagcagc ttggtcggtg
   121021 atatcttcta ggaaatgata gatcgctcct agttttgtat aattattgat atcgtaagcg
   121081 atagctggaa ctttttcagc agctgcttcg atttcagcaa ccgaacgttt tgtcacacgt
   121141 gcagcacttt ctttagacat cgcttggacg gtaatttctt tattgtttaa agcaatcaag
   121201 cggcttgatt gtaatccatg tgctaaaaat gcacctgaaa tagcatttcc tgcaatcaat
   121261 ccaataactg gatgtccagc ttgacggagt tttgcataag cagcagcact attagccaaa
   121321 gccacatgga taccgatcaa ttcttcgtta tagccgtatg cttggcttgg tacatcgatc
   121381 acaacgatga ttggccgttt cacttttttg tctttatctt cttcgtaaac gtgatccaaa
   121441 atttgtccca tgcggaaacc ttcaactaaa ccaacttcac cgtttcgtac acggtacatc
   121501 gggtttttag gatcaggtac gattgatgtc acgatggctt cttctatgaa tttacttgat
   121561 ttaccataat acacagtact aatagaagaa gttggatttt tcatgccagt tagtgccgca
   121621 aaccaatcgt aaccaacact tgctgcagct ggttcatttc cttctgctac ttctggtaag
   121681 tcaattgatt ttgcttgatg gttagcaatt gcttcattgt aatcgtcaat ggccattggt
   121741 tttgaaagat ctaaagaatc aagtaatgat aaataaaatg ctgagctttc actgcgatgc
   121801 gtgtcttttc gttcgtccat tgctgcgatg acagcagctt tgattgcttc tacactatca
   121861 gtcactaatt catcaatgat ttttgattct acccgttgac gagatcctaa tgtattccaa
   121921 atcaagtctt tgtcactaga atcaaattct cgaacgcctg cttcttgttc aataacttct
   121981 ggtccattca aaccgactct cgcttttttt gttgcgatta aataactcat taatgctgaa
   122041 gtaatcgaca taccgccaaa tgatcctaca cgtcctggaa tcaaaccgat gactggtaca
   122101 tattgtttta gagcaatgat tacatttccg atttctgaaa tagataacag tccgtagttt
   122161 gcttcttgca aacgaacacc acctgtatct agtacaatga ctggaaagat ctcatttcct
   122221 tttttatttt cttctaacac atgttcaagt gcagcaacga tttttgcacc ggaaacttca
   122281 ccgataccgc caccttggaa ttttccttcc atagcgatta ctactgcttg tttgccattg
   122341 atcgtgccac ggctgacaac gatcccgtca tcactttcag ggacgattcc ttgtgctggt
   122401 aaacttggcg cgatgatttg atcgaatgga tcaatcaatt cacgtccatt gtcaagtaga
   122461 gcgttcactc gttctctggc gtttaattct acaaaactat ttttcatctg aaagttcctc
   122521 cattgcttgc gtcaatcgta ggtacacaac tcctggtgta gcaccgaagt cattgatcac
   122581 gatatctgct gaaattggat aacgatcaaa aaatctcgtt aatacattgt tccatacttc
   122641 gccgaagcca tcgcttcctg tacaaatttt tacagtagat tctgtttttt ctgttggaaa
   122701 catcaagatt tccaaatctc ctgaaccgac cactcctaca tggattttct tattgatcgg
   122761 ctgtttcgtt ggatatttat aagctaattg ttccatggat ctctcccctt tttgtaaaac
   122821 agagcataca cgttctgctt attcgtcttt cttcttccat ctgggtaaat ggcgacaaaa
   122881 ggtttttctc gccaatttta tgaccaagtt ctgaatttca ttggtggttc atacaggcca
   122941 tctgaccatt cgatcaaatc atcgatggtt tgtgcagcta ataatgaacg tttcgcttca
   123001 ctacgtttga tgcctagatc ttctggtaat ttcacgatgc cggctgcacg caagctatct
   123061 agtgattctt tcgtgctagt cattccaaga ggtgtaacac cgccaattgc tgcaattgct
   123121 gcttgtcgtt cttcttttgt ttttgctttg tacaaataag caattccttc ttctgtcacg
   123181 atatgtgtag tatcttcgct ataaatcatc actggtgcat tggctaagcc tgcttgtttt
   123241 tgaacagcta gtgcatctaa ttcttcgaca aagactggtt ttttatttga accatatgtt
   123301 tcaaccattt gaacaactaa tttttggcct ttccctaatg gatcatcatt gtcgcgaagt
   123361 gattgccatg ctggtgtaga atgacgacga ccgccaggat tatgtcccat gttaggcgca
   123421 ccgccaaaac cagataaacg tccttttgtt acggtagaag agtttccttc gtaatctaat
   123481 tgcaaggttg aaccgataaa catatcgatg gcatattgtc ctgcagcttg tgataatgtt
   123541 cggttactgc gcatcgtgcc gtctttaccg acaaaaaaga catctgggcg agcagcaata
   123601 tatttttcca taccaacttc gccaccaaaa ctatggatgc tttcaaccca acctgattcg
   123661 atggctggaa tcatcgttgg atgtgggttc aatacccagt tcttcgcgat tttccctttt
   123721 aaccctagtg attcaccata agttggcagt aacaattcga tagctgctgt attgaatcca
   123781 ataccgtgat tcaaagattg tacgccgtgt tctgcgtaga ttcctttgat tgccatcatt
   123841 gccattagaa tttgcaattc tgtgatgttt tgaggatctc gtgtgaataa agcttctaat
   123901 tgataaggtt tatctgcttt cacgacaacg tctacccagt caccagggat atctacacgt
   123961 ggtaaagaat cgacaacttc gttgacttga acgatcacga taccatcttt gaaagctgca
   124021 gcttcgatga ttgttggtgt ttcttccgta ttgaaacctg tgtacaagtt gccgtcttta
   124081 tctgctttat ctgcagctac taaaacaaca tttggaatca aatcgatgaa taagcgtcca
   124141 taaagttcca aataagtatg gatatcacct aatttcattg tgccgtcttc aatcatttgg
   124201 gcgacacgca agctttgtgc accagcataa gagaaatcga tcttgcttgc gattcctttt
   124261 tcaaaaatat tcaaatgttc tggtcgtgag atactagaca tgatcaaatg cagatcattc
   124321 actttttctg catctacttt ttcaagactt tctgaaagaa aacttgcttg tttttgattg
   124381 tcgccttcta atacaacttt gtcgccggga gcaatcagtt tttccattac ttctacgatt
   124441 ttttcagttt ctacatactt gccattgagc aagttgctga ttttttccaa tcgctgcgct
   124501 tttttttcgc gtttagaagc ccagttgcgg tttggttttt ccataattag tcatctccat
   124561 ttttgatata gaaacgtggc gtcatcaata ccatgttggt ctagattgat cgtacggatc
   124621 cctttatcta caccaaattt tgtatgaatc agctttgtca aagtattgcc ggggggaaat
   124681 tcaatcgtca gatccattcc taattctttt gccaagtcca tcatttggtt ccactgcaca
   124741 gggaaagcta ggttttccaa caaatcttta cgaatctctt ctgcgtcctg agtggctctt
   124801 ccatccgtat tttttatata caaacatcta gcatcagata gatggacaga ctgcatatac
   124861 ggcttgaatt tcttgaccgt atcattcatc aagagacagt gagatggcac agggactttc
   124921 agcagcttcg ctgtttgcgc ttggtgccgt ttggcttctg ccaacgtaac ggataaacct
   124981 tcgatactcc cagataacgt atgttgcatc ggacaatttt catttgacag ataaactgga
   125041 tggtcaggat caaatgtttt tgctacaatt tcctcagctt cgcttctcgt cacacctact
   125101 actacgccca ttgcataacc gcttggataa gcttctttca ttaagactgc gcggtgatag
   125161 actagttccg ctgcattctc aaatctcaaa gatcgacaag caacagctgc acaaaaagca
   125221 ccaatcgaat gtcctgctgc aatatcaggc tgaataccgt ttttttccat ttcatctaaa
   125281 taaaatactg cttttgtcag taatgctagc tggataaaaa ccgtatcttg atagttttct
   125341 tctttatcaa ctaaccgata ccccgtcaca ttttctaaac gttccatgta catttcaggg
   125401 atatccgcta acatccctaa cttctgactg ccttgtccag gaaataagaa acctgtactc
   125461 atgagcctcg cctcctcagc acttactttt tccactgcta tcttagtcaa tttctgctat
   125521 aataaaaaat gccaaaaaaa gaaaaaacca taacaaaaag gtatggatgg gatggataat
   125581 tatgaaattg cttcatctgc agtattttaa aaaagtattc gaaacaaaaa atgttaccca
   125641 agcagccaaa gaattattta tttctcaacc tgccttgagc cgtgcgatca aacatttgga
   125701 aaacgaatta ggtgtccctt tgttttacca tagcggcaga aacattgaga cgaccgttta
   125761 tgcagaagaa ttttatccat atgcagtcaa gacattagac acattagaag aaggaatcaa
   125821 attattgtct tcggtcaatg agaagatcgt tacttccgtc atattgtatt tagaggtagc
   125881 ctctgtttct attccgaact tggtccgaat ttttatggaa aagcatcctg atatccaatt
   125941 aacgatcatg caacatcatt taccagatga tattaccgat cctgtactgt acatcacttc
   126001 ggagatgaag cctggcatga ccaatattcc tatcatgaaa gaacctgttt atgtcgccat
   126061 tcctaagaaa catcctcttg ctaaaaaaga acatctgcaa ttagaagata tccaacgcac
   126121 ccccatcttg atgctgtcaa agagcaacgc cttccgtcat acactggatg cagcacttga
   126181 agcgaaaagt atcgaattga cgatcagttc gacgacagat gatcctgcga cattacgttc
   126241 tattgtcaag caaggattag ctgttagctt tttcccaaaa gtttcttggt cttacgataa
   126301 aaatgatcct ttcgtcttgc gggaaatcaa agatttgccc ttgacacgaa caatttattt
   126361 atcttctagc ttcacagaag atcatccttt gatcaaaatg attgcaaaaa cgatgcggga
   126421 attttattta ggaaaataaa aaaaaaagag ctgggacaaa attcttgtcg cagctctttt
   126481 ttcgaacaaa cggtgttcca gaagtaattt cttagaaaat aagccgaaat ttcccaaaaa
   126541 tttgagaagc aattttcgta aattccttct tatttcttga agctaaacat ttctgtcaca
   126601 acctcttctc tattgtccta gacattcatt gcgttgattt aaaacttcca tcaatcaaat
   126661 ctgctagttc atctagcttt tctattttac gtagatcaat agagatccaa tgttctttat
   126721 tcatgtgata ggctggaaaa tacccctctt ttcctcgcaa aattccgcca agttcaggat
   126781 cgaccttcaa atcaagaata ttgatgatcc tatggtcact tgcatcatca tagatttttt
   126841 cttctggaac agacatcagg accccaaacc attttttgct tttttgatgt ctgaatacgc
   126901 aataatcagg aaatttttta aaaatatatt ctggagacaa attgtatgtt tcattgatgt
   126961 gatcgattag ttcttttttc gttagcatct gcttccatct ctttcttatt gattcgcttt
   127021 attcaaagat accacgtaag aatagaaaaa tgaatacaag tagttgctaa tcaaatactt
   127081 cgatattttt cttttttatc aactcttaaa tgttcaatcc gcctgtgttg gtcttgctac
   127141 catctactat taaaatgttt attattgttt gcccacctat tttttgattt tagtggtatc
   127201 atttttcaag ttcatgatga aattcttatc atcagtaaat gctccagaaa aataaacaat
   127261 gattgaaaaa tcagttgaaa cttgaccacc gtttattttc tccgtattct tagaatagaa
   127321 gggagtctga ttgatgtatt cactaatgaa aaaaatcatt accgaaaaag atattcgacg
   127381 tcatgtttct ttagtagaac aattgctgaa tcatacaaag ataaccgtaa atgaattagc
   127441 tgaaatcata gggacaaccg aacgaacgat tttttctgac cttcaatcta ttcgctctca
   127501 gctgcccgaa ggctgggaca tcatcagcga tcaagcagga attagtttgc aaaaccagca
   127561 aaatttatta acaaatgacc tttgggagat ttttttcaaa caatcagtca gtgtcgaact
   127621 gctgaaaaat ctgcttttta caaaaaaagt tgctgtccct gattttctag ccgaccatgg
   127681 gctttcttat gggacattga aaagacatgt gactaaaatc aatcagcgtt tagcaagtta
   127741 cgacttgcag atcgatttga cgaaatatac agcttgtatt cttggaaaag agcgtgcgat
   127801 tcgtactttt tatcatcgat tgctgattcc tttcactcat aacaactatt ttttcgagga
   127861 ttattcgatc catgaatccc attattttca atttttaaga aacctcagtc aaactgaact
   127921 agcggttgaa acagaagaga ttttcggaac atgttggttc tttatcaata cgatccgtat
   127981 aaaagcgaat tgccgattag attcttctat ccataccaac agcacactct cctccctgta
   128041 tgactctgca ttgaaaaaat tgtatttaac ggaaggtatt tatcttaaag acactgagct
   128101 ttcttttgct tctttctgct ttctggaaag ttggaattac aacaataact atggacaaga
   128161 aaccgctcgc tgcctgcatc attctccatt tttagaggtc ctcgaaacgt ttgtagagga
   128221 actagcttca gagctgtctc ttgatcaact aaaaaaaaca tctctgactg ataacttggc
   128281 tctcctcata ttaaaatatc atgaatcgcc gatattgatt gaagcattgg accgtcaata
   128341 ccatcgtttt ttagaaacat atgatcagca atatgcacac ttgtatcaac agaaagagaa
   128401 tttactgaac gatctaaaaa aagaaataga aatcaatgaa ccaaattact ttctccaact
   128461 cttgtctcta ctgatccaac aaacgatttt ttctatcaaa ccgaatttgt tcaatgtcta
   128521 ttttattttc caaggagaac cttcatggaa ggcgttcttg cagcaggaat taaaagatta
   128581 cttaggaaaa cgtgtctgct tcttacctat cgaactaacc gatctttcca ctatttcttt
   128641 tgaaaaaaca gatatcttga tttccaattt ccctctagat tctctagata tccctattgt
   128701 atacatttct tctattccaa cgaaaaatga gctcaatcgt ttgactgaac tcactttcaa
   128761 aactttttta tagtaacggc tagcgactgt attctctagc tgttttttct tttttgcgaa
   128821 acagtttgtt catgatccct ttttctattt ctacaactaa gaatagaaga atcacatttg
   128881 ctccaatcaa taactgttgt gtcatcgtta aacttactgt accgatcagc tgctggccga
   128941 ctggtagaaa aataaccatc ccttgcaata aagcaaggat acctaatgaa agaaaaagcg
   129001 ctttgttttg caaaagacct tggttcaatg acggcttgga caactctctg caattgatca
   129061 tgtaaacagc ttgagctaag acgatatttt gcagaagaat ggtttgctgt aagctttgcc
   129121 cttcaaactg catagccaat aaataggcag gaaccatgat cagtagggag acatagatga
   129181 tccgaaacaa atgataacca gataggattc cttgctttgg atctcttggt gggcgcttca
   129241 tgatttctgg atctggcgac tcgaacccta gttcataaga taaagtgatt gtcgttacca
   129301 tattgaccca taaaatctgt actggggaca aaggtagcgg gcgattcagc ataagtgccc
   129361 aaatcaagat caatccttga gctaatgcag ttggcaagaa gaaattgatg gttttttgca
   129421 aattatccaa aatccgccgg ccctctttta cggcttttgc aatcgtatgg aaattatcat
   129481 ctcctaaaac catatcagct gcctgtttgc ttacttcact acctttgatt cccatagcaa
   129541 ttcctacatc ggatcttttc aaagcaggtg catcatttac gccgtctccg gtcattccga
   129601 cgatttctcc atttttctgc aaagctttaa caattcgcag tttatgttct ggtgtcgttc
   129661 gagcaaagac atcgactatt ggcacttgct gaatcaactc ttcatcagac ataagatcca
   129721 aatcgatccc ttctaaaact ttggcagtat gttttaagcc aatttgttcc gcaatcgctt
   129781 ttgctgtgtc tttgtgatcc cctgtaatca ttttcactga gatccctgct tgttggcatt
   129841 cctttacagc tttgactgca ctttcttttg gcggatcgat caatccggcg atccctgcaa
   129901 aagttaattg ggttaaagtc tcatgcgtga gttcctgttt cgaatctact tttttataag
   129961 caaatcctaa aacacgctga ccttttctcg caaattctgc agctgtctga ctccatgctt
   130021 cttgttctgc agaagaaaga gttgatttag caaataatac ttctggtgca cctttgacgt
   130081 aaatcaccgc tcctttttcc tccgcatgca tcgttgccat atatttgtat tcagaactga
   130141 acggaatctt tgcttctatt tttttcaaag gtagattggc tgtttctgtg tgtcgaatca
   130201 gcgcaacttc tgtcggattg ccacttaaat tttctgtttt ctgttcttct tttgttttta
   130261 attcctgaca gtttttcata atggacaaca cgcaggcttc ttcttttgca gctacatcca
   130321 caactgtcat ttcatttttt gtcaatgttc ctgtcttatc tgaacagatc actgtcatag
   130381 aacctaatgt ttctaccgaa ggaagtcctt tgatgatcgc cttttctttt gccatttcat
   130441 gtactccaac ggatagaatc atggtcaaaa cagctggcaa tcctttgggt accattgaca
   130501 ccaccaaagc aatcgtagcc gaaagaagga tatgccaatc cataccatat cgaaatgtcg
   130561 taaaaataac taagaatagg ctcaaacaaa gaatgccttg aaaaatttgt ttgtttaatc
   130621 ggttgatttt tttaatcaga ggcgtcgttt gttgttcgac cgattgcaat gcttgattga
   130681 ttttaccgat ttctgtcgtg tttcctgttt ctacaacgac accgactgca gaaccagatt
   130741 gtacaagcgt tcctgaaaaa gccatatttt tctggtcgcc gatcgattct tcttctgtca
   130801 aaacggcatg acttttttca gatgcttctg attctcccgt caaaatcgat tcttccacta
   130861 ataaatcatg tgcttcgatt aatcggatat ctgctggaac gacatctccg gcacgtaaga
   130921 caatgacatc tccttttacc agttctttcg cagggatttt ttcttgcatc ccgtttgata
   130981 gtactatcgc ttcttgtccc atcagctgct tcaaaccatt caatgattct tccgcttttc
   131041 tttcttgcca gtaactaacg agtccattga cgatcactac gaataaaata atacttcctt
   131101 caatatattc actagaagca aatttcaaaa aagaggcaat gatcaacacg atcatcagca
   131161 gatcagtaaa atgctttgct atttttttcc atcgttttgt ctgctgcctt gcttttattt
   131221 cattatatcc ttgatcagtc aggcgtttct gtcgttcttc ttgcgacaag ccttctgttg
   131281 atgtttgata gtttttcaat acatcttcta ccgtcaatct gtaccagttc atcctatccc
   131341 ctcctgttta attgatactg ttattatacg cagggaagtt ttttcaggcg tatcgattat
   131401 ttcagggggg tctgaaataa ttcgttttgc aaaatgtatc tgggcctaac tgatatttct
   131461 attgcttatt cttttatata caaaatttaa gaatacaaac cagataacca ataaaaaagg
   131521 aataaagcgt gtcgtgcttt cgaataacaa gctgaaagca attgaagtaa aaaacaccaa
   131581 caaagcaaga tctttatatc tttgtgtagt gttccgtttc ttttctttaa taaattgaac
   131641 atgtgcacac aagatcgaag accacacaaa tagaaaacaa ctcgttgtca tgcctgccaa
   131701 aagctgaaaa aacaatcgac tatcagctac aaaaaaatgt agacaaagac cggcaaaaaa
   131761 taacattcct gtaattaaaa taccgtgtaa aggtacgcct ttcttagaaa gcgcacaaaa
   131821 ccaatggggc aaactttttt cttttgctgt ttgatataaa atcctgctag tactgtagac
   131881 tgcactattt gctgaggaca aagaagcaac caaaatcatt agattgacaa ttgctgctcc
   131941 gccttggcaa tggatcactt gcagcatagt cactaaagga ctttctttta aatctaaaca
   132001 atgccatgga acaacaaata aaatcgacaa aatcgttcct atatagaaaa agagaatacg
   132061 ccaaggcaga cagttgattg cttttttcaa attcttttgc ggatcacttg tctcgccaac
   132121 ggttaagccg atcatttcaa ttccagcaaa actgaataaa accataggaa atgagactac
   132181 cattccagca aagccaaacg gtagtaattc atcgatctgt ccaaaatttt ctttgattcc
   132241 gatagactgg gtatgaaaat ctgagacact caatcctata cccaataaga taaaaaggaa
   132301 aatccccaag atcttgatga ttgagaaaaa agattccaat tcaccaaacc acttgactgc
   132361 aacaaggttg actgccatta agatcattag tgcgctacct ccgcttatca aagctggtac
   132421 atgtacaaac cagaaatgga tatacaatcc tattgctgtt aattccgcaa gtccagctcc
   132481 aagccagcaa agccaataag tccaattgac aaaaaatgcc cactttttgc ctaaataata
   132541 gtcaacgaaa tcactgatag agttaaatgt actatttgct aaaaataaat cacccatggc
   132601 ccgcatcaaa ccataaatag ccaaaccgca tatcaaataa acgagaataa cagctggccc
   132661 tgctgatgca attgcctttg ctgaacctaa aaataatcct gttccaattg cgccacctaa
   132721 tgcgattaac tggatatttc gattagttag agtccgtttc attgccggtt tttccatcaa
   132781 gttcaccttt ttctttttca agcagcatgg cgacttcctg ttcccaagaa ggtttcttta
   132841 ttttttcaat acagagtggt actacaaaca aaatgacact gatggctgtg actgcaatta
   132901 gattgatcca tgttccagct ttcatcgtca gtccgacaga aaatccgatt gccgcaaata
   132961 aaacaataac gacagtccaa actccaagat tgctcttttt ctcttttttt ccaattctaa
   133021 atggtcgatt caactttgca tgtgtataac gcaatctcaa aactgcgatc cccatgatga
   133081 catagacgat acagtagata acagtagttg aatttacgag catcaaaaat gcttgattga
   133141 cgccaggaat cagcaaataa aaaagtgcaa agattgagat aatgacagac tgagtcaaaa
   133201 taacggattt ggatacgtca aattgattcg tcttccaaaa attgaatttt ggtggatacg
   133261 caccttttct agcactttct gtcactgtct ttgctggtcc tgaggcccaa gcagataatt
   133321 ggactgctac accaatgaag acaagtaaac taaacagatt cacaataaga tgtggcaaac
   133381 caagaatttg gcaataaatc gaaatagatt gcgcgatatt attcaattcc atttgtcctt
   133441 ttggtacgac atttgctaca actaaagcat tcaaaatatt gacgaaaaat ataaaaatta
   133501 acgcggcaaa tactccgcga atatacgttt taactggatt ttccaatcga gtgatataca
   133561 cagaggacat ttcgatacca gtaaaaataa acatgatcgg cgcaaaataa acaaatgagg
   133621 atgcagtcgt agtatctggt attaatttgt cccatgaaaa actgcctaat gttccggttg
   133681 gtacgatccc aactttgatc cacgcagcaa accctaataa gagcatcatc gtcaaaggca
   133741 cgtacaatcc taaccaaaca ccgacttttc cgccgatttt tgccatatca aatttcaaat
   133801 tcaaaaaagt gatgatccaa taaacgacta aaatgacaac taatgtaaat ttgctgttca
   133861 aaccaagcgg cacattatcg ataatattac caaacaacgg agcgagtgct gaagcgacca
   133921 tcaccatccc aggaaacatt tggacccata acagccatga gacaacgaac ccccattttt
   133981 ttcctagagc attcgatacc caaagttctg gaccaccttt ttggggaaac attccagcaa
   134041 attcaccaga gatcaatgag attggaaaag cgtacaataa tactgcaacc agcatgtaaa
   134101 aaatcatcgt ccagctcgtt gctgctacat ctggaatatt tcgtacactt gctacaaggg
   134161 cgcaggtcat tccgataaag gtcatggtcg tgatttgctt tgatgaacca gtatctttca
   134221 tctttattcc ttctttcaat agcgacatat gtttttgttt tcttttattt ttgttcaaga
   134281 acagaaatga caaggtttca tcgattcagt acaaaaccca gtatcaccga aacatccatt
   134341 gaacgatctt agtgaactgg gtttgataca tgaaacgaac gatccaactt atagaacgtt
   134401 ttataaaaaa gaatatcttg gcgaggaata tatgaaaaac agggggaaat acaattgttt
   134461 tgatgaagca aataattgtc tgttctccat atgtctcttg ttattctttc acacaaaaat
   134521 cagttctatt ggatgatttc gtttagtttc ttttccattg ctttggcgat tttttttgta
   134581 taaaaattga aaattcgatc atcattcaaa taaggagtca tgatagccgc acgtagcaga
   134641 gtgattttct gttctttttt ccattcttct ttcgttagac ccattctttc aacaaaacga
   134701 atcggagaat caccgtaatc ttcttgtgta aatgtagtat gagaagtaat aaaccgttca
   134761 ccataaacat cgccatccat ataagaagac cgatcgaaca ttttttcatt taattcattg
   134821 atagcgttca aatctgtaca gccttgttct ttgaataccc aattgaccat attaaaatca
   134881 ggatgattca atggatagac ttcgattgtt tttcctttaa cagtaaaagt cagatgatcg
   134941 agaaattctc tgaatctttg agctgcctcg attgaagccc caatcaattg accatatccg
   135001 gtcacattca atggcaatac acgatgtgct gtccagactg cggcggcggt tgctccagct
   135061 tttgatcctt ccaaaatata agctcctaac atatctggag cctttactga tttttcaaac
   135121 acatatggcg cgaaataaga aatgatattt ctcatatttt tatgtttgat tacgattcct
   135181 ccagcagcat aagggacata gcccatctta tgaggatcta tcgtgacaga atccgcttct
   135241 gaaatagctc tgaacccttc atatacctct ttgtcaatcg ttacgcagtg gtggaaaaca
   135301 tgatgttctt caaagaattc agctaaggaa gcatacggca caaattctcc tgcttcgttt
   135361 agaaacagtg aacgcgcata gccgccatat gcggcatcaa catgtaaata gaaatagatc
   135421 ccttcatctt ttaacctttc tcttaattgg acgattttat ctacactgtc tacttgacct
   135481 tcttctgtcg ttcctacgac agcaaccaca cctagaattg ggattttttg tccagctaat
   135541 tcacgaattg ttttttccaa agcattaata tccattcgat aatcctcttg aactgggata
   135601 gcaaccatct gatctaagcc gactccgcaa atatctaacg ccttcatcca agaatagtgt
   135661 tttgtttgag ggaccagcca tttccctaat ctttggatat tttttccgct acgggaagaa
   135721 gctgctttga cctcatccat ttcttcgtcc gtaaagcttt cggtcatttc caaaatttct
   135781 tcaacagata aattcaaaag ttcccactca gacatttttt tcactttttc aggcagcact
   135841 tccttgacag ctaaaggaat cgatttgatg caacgggcgt accataatcc ttcaagatta
   135901 gcaatagaac catctgctgc aatgtgtccc catccgtctg tcatattgaa taatgaagca
   135961 aaatcctgtc ctacttctgc ttccatttgc gaagttgcca tagaagattc caatgcaaca
   136021 ttatttgggt tccacagcat cgcatagttg taagctaata aagcaggcat caatgtttca
   136081 gcattcattt gtccccagta acggcctgct gaatgccatg ggatcgatcc tgcgcgtaat
   136141 cgctgactga cttcatccaa gacctctatc atatggtcgc gggtggctag ataatctgga
   136201 gtgagcttat ctccttcgct aattgcaggc atatctgaag gaatataatt ttctctccat
   136261 ccaagatgtt catctaccat cttgttcaac aacattttat aaactggtcc attttcagct
   136321 ttgtcgccta taaagacggc cttgatatcc atatctttca tttttcttcc ctccatattt
   136381 tgttggtcaa tgtcttgcag atacatgtgt ttatctgtaa cacctcttca ctatagcgcc
   136441 aaaaatgaga aatttttttt tataacatca aatccacgta gatttttaca aaca
//
LOCUS       DACDPS010000002        97294 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.002, whole genome shotgun sequence.
ACCESSION   DACDPS010000002 DACDPS010000000
VERSION     DACDPS010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 97294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 97294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..97294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.002"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IWJ07_000118"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IWJ07_000118"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAP9331729.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IWJ07_000119"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IWJ07_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAP9331730.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IWJ07_000120"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IWJ07_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAP9331731.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IWJ07_000121"
     CDS             complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IWJ07_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAP9331732.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IWJ07_000122"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IWJ07_000122"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAP9331733.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IWJ07_000123"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IWJ07_000123"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAP9331734.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="IWJ07_000124"
     CDS             complement(8053..8523)
                     /locus_tag="IWJ07_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAP9331735.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="IWJ07_000125"
     CDS             complement(8569..8994)
                     /locus_tag="IWJ07_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAP9331736.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="IWJ07_000126"
     CDS             complement(9061..9330)
                     /locus_tag="IWJ07_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAP9331737.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="IWJ07_000127"
     CDS             complement(9341..10936)
                     /locus_tag="IWJ07_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9331738.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IWJ07_000128"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IWJ07_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAP9331739.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="IWJ07_000129"
     CDS             complement(14489..15049)
                     /locus_tag="IWJ07_000129"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAP9331740.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="IWJ07_000130"
     CDS             15402..16376
                     /locus_tag="IWJ07_000130"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP9331741.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="IWJ07_000131"
     CDS             16546..17946
                     /locus_tag="IWJ07_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAP9331742.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVNLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="IWJ07_000132"
     CDS             complement(18024..18746)
                     /locus_tag="IWJ07_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9331743.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="IWJ07_000133"
     CDS             complement(18825..19331)
                     /locus_tag="IWJ07_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP9331744.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="IWJ07_000134"
     CDS             19454..21253
                     /locus_tag="IWJ07_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAP9331745.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22895)
                     /locus_tag="IWJ07_000135"
     CDS             complement(21321..22895)
                     /locus_tag="IWJ07_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9331746.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="IWJ07_000136"
     CDS             complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="IWJ07_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAP9331747.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="IWJ07_000137"
     CDS             complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="IWJ07_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAP9331748.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24672..25310)
                     /locus_tag="IWJ07_000138"
     CDS             complement(24672..25310)
                     /locus_tag="IWJ07_000138"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAP9331749.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25311..25961)
                     /locus_tag="IWJ07_000139"
     CDS             complement(25311..25961)
                     /locus_tag="IWJ07_000139"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP9331750.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="IWJ07_000140"
     CDS             complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="IWJ07_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAP9331751.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="IWJ07_000141"
     CDS             complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="IWJ07_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAP9331752.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29104..29844)
                     /locus_tag="IWJ07_000142"
     CDS             complement(29104..29844)
                     /locus_tag="IWJ07_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAP9331753.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="IWJ07_000143"
     CDS             complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="IWJ07_000143"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAP9331754.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31215..32153)
                     /locus_tag="IWJ07_000144"
     CDS             complement(31215..32153)
                     /locus_tag="IWJ07_000144"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAP9331755.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32146..32637)
                     /gene="def"
                     /locus_tag="IWJ07_000145"
     CDS             complement(32146..32637)
                     /gene="def"
                     /locus_tag="IWJ07_000145"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP9331756.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32652..35060)
                     /gene="priA"
                     /locus_tag="IWJ07_000146"
     CDS             complement(32652..35060)
                     /gene="priA"
                     /locus_tag="IWJ07_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAP9331757.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35313..35618)
                     /locus_tag="IWJ07_000147"
     CDS             complement(35313..35618)
                     /locus_tag="IWJ07_000147"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP9331758.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="IWJ07_000148"
     CDS             complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="IWJ07_000148"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAP9331759.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(36724..37008)
                     /locus_tag="IWJ07_000149"
     CDS             complement(36724..37008)
                     /locus_tag="IWJ07_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9331760.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(37026..37916)
                     /locus_tag="IWJ07_000150"
     CDS             complement(37026..37916)
                     /locus_tag="IWJ07_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAP9331761.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(37936..38154)
                     /locus_tag="IWJ07_000151"
     CDS             complement(37936..38154)
                     /locus_tag="IWJ07_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9331762.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(38124..39824)
                     /locus_tag="IWJ07_000152"
     CDS             complement(38124..39824)
                     /locus_tag="IWJ07_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAP9331763.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            40091..40864
                     /locus_tag="IWJ07_000153"
     CDS             40091..40864
                     /locus_tag="IWJ07_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9331764.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(40945..41514)
                     /gene="thiT"
                     /locus_tag="IWJ07_000154"
     CDS             complement(40945..41514)
                     /gene="thiT"
                     /locus_tag="IWJ07_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAP9331765.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(41580..41682)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(41688..42659)
                     /locus_tag="IWJ07_000155"
     CDS             complement(41688..42659)
                     /locus_tag="IWJ07_000155"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP9331766.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(42753..44126)
                     /gene="glmU"
                     /locus_tag="IWJ07_000156"
     CDS             complement(42753..44126)
                     /gene="glmU"
                     /locus_tag="IWJ07_000156"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAP9331767.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(44629..45447)
                     /gene="purR"
                     /locus_tag="IWJ07_000157"
     CDS             complement(44629..45447)
                     /gene="purR"
                     /locus_tag="IWJ07_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAP9331768.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(45614..46438)
                     /locus_tag="IWJ07_000158"
     CDS             complement(45614..46438)
                     /locus_tag="IWJ07_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP9331769.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(46411..47091)
                     /locus_tag="IWJ07_000159"
     CDS             complement(46411..47091)
                     /locus_tag="IWJ07_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP9331770.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(47114..48052)
                     /locus_tag="IWJ07_000160"
     CDS             complement(47114..48052)
                     /locus_tag="IWJ07_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAP9331771.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(48297..49148)
                     /locus_tag="IWJ07_000161"
     CDS             complement(48297..49148)
                     /locus_tag="IWJ07_000161"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAP9331772.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(49302..51875)
                     /gene="mprF"
                     /locus_tag="IWJ07_000162"
     CDS             complement(49302..51875)
                     /gene="mprF"
                     /locus_tag="IWJ07_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP9331773.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            52016..52822
                     /gene="proC"
                     /locus_tag="IWJ07_000163"
     CDS             52016..52822
                     /gene="proC"
                     /locus_tag="IWJ07_000163"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAP9331774.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(52901..54457)
                     /gene="rny"
                     /locus_tag="IWJ07_000164"
     CDS             complement(52901..54457)
                     /gene="rny"
                     /locus_tag="IWJ07_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAP9331775.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(54868..55917)
                     /gene="recA"
                     /locus_tag="IWJ07_000165"
     CDS             complement(54868..55917)
                     /gene="recA"
                     /locus_tag="IWJ07_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAP9331776.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(56026..57267)
                     /locus_tag="IWJ07_000166"
     CDS             complement(56026..57267)
                     /locus_tag="IWJ07_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAP9331777.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(57507..58085)
                     /gene="pgsA"
                     /locus_tag="IWJ07_000167"
     CDS             complement(57507..58085)
                     /gene="pgsA"
                     /locus_tag="IWJ07_000167"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAP9331778.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(58294..59178)
                     /locus_tag="IWJ07_000168"
     CDS             complement(58294..59178)
                     /locus_tag="IWJ07_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9331779.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            complement(59956..61242)
                     /locus_tag="IWJ07_000169"
     CDS             complement(59956..61242)
                     /locus_tag="IWJ07_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP9331780.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(61235..62503)
                     /locus_tag="IWJ07_000170"
     CDS             complement(61235..62503)
                     /locus_tag="IWJ07_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP9331781.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            62755..63216
                     /gene="mscL"
                     /locus_tag="IWJ07_000171"
     CDS             62755..63216
                     /gene="mscL"
                     /locus_tag="IWJ07_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAP9331782.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(63275..63721)
                     /locus_tag="IWJ07_000172"
     CDS             complement(63275..63721)
                     /locus_tag="IWJ07_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9331783.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(63814..64059)
                     /locus_tag="IWJ07_000173"
     CDS             complement(63814..64059)
                     /locus_tag="IWJ07_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9331784.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(64174..64752)
                     /locus_tag="IWJ07_000174"
     CDS             complement(64174..64752)
                     /locus_tag="IWJ07_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9331785.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(64829..65359)
                     /locus_tag="IWJ07_000175"
     CDS             complement(64829..65359)
                     /locus_tag="IWJ07_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAP9331786.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(65363..66223)
                     /gene="rlmB"
                     /locus_tag="IWJ07_000176"
     CDS             complement(65363..66223)
                     /gene="rlmB"
                     /locus_tag="IWJ07_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAP9331787.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(66216..66611)
                     /locus_tag="IWJ07_000177"
     CDS             complement(66216..66611)
                     /locus_tag="IWJ07_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP9331788.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(66608..68020)
                     /locus_tag="IWJ07_000178"
     CDS             complement(66608..68020)
                     /locus_tag="IWJ07_000178"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAP9331789.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(68017..68553)
                     /gene="cysE"
                     /locus_tag="IWJ07_000179"
     CDS             complement(68017..68553)
                     /gene="cysE"
                     /locus_tag="IWJ07_000179"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAP9331790.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(68875..70335)
                     /locus_tag="IWJ07_000180"
     CDS             complement(68875..70335)
                     /locus_tag="IWJ07_000180"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAP9331791.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(70505..71662)
                     /locus_tag="IWJ07_000181"
     CDS             complement(70505..71662)
                     /locus_tag="IWJ07_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAP9331792.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(71696..73066)
                     /gene="radA"
                     /locus_tag="IWJ07_000182"
     CDS             complement(71696..73066)
                     /gene="radA"
                     /locus_tag="IWJ07_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAP9331793.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(73129..73656)
                     /locus_tag="IWJ07_000183"
     CDS             complement(73129..73656)
                     /locus_tag="IWJ07_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAP9331794.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            73886..74692
                     /gene="proB"
                     /locus_tag="IWJ07_000184"
     CDS             73886..74692
                     /gene="proB"
                     /locus_tag="IWJ07_000184"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAP9331795.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            74689..75936
                     /locus_tag="IWJ07_000185"
     CDS             74689..75936
                     /locus_tag="IWJ07_000185"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAP9331796.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            76012..76356
                     /locus_tag="IWJ07_000186"
     CDS             76012..76356
                     /locus_tag="IWJ07_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9331797.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(76473..76799)
                     /locus_tag="IWJ07_000187"
     CDS             complement(76473..76799)
                     /locus_tag="IWJ07_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9331798.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(77414..78616)
                     /locus_tag="IWJ07_000188"
     CDS             complement(77414..78616)
                     /locus_tag="IWJ07_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9331799.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            78733..79248
                     /locus_tag="IWJ07_000189"
     CDS             78733..79248
                     /locus_tag="IWJ07_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9331800.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(79447..79893)
                     /locus_tag="IWJ07_000190"
     CDS             complement(79447..79893)
                     /locus_tag="IWJ07_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP9331801.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(79940..81418)
                     /gene="msr"
                     /locus_tag="IWJ07_000191"
     CDS             complement(79940..81418)
                     /gene="msr"
                     /locus_tag="IWJ07_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP9331802.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(81772..82401)
                     /locus_tag="IWJ07_000192"
     CDS             complement(81772..82401)
                     /locus_tag="IWJ07_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP9331803.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(82385..82849)
                     /locus_tag="IWJ07_000193"
     CDS             complement(82385..82849)
                     /locus_tag="IWJ07_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9331804.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(83336..84604)
                     /locus_tag="IWJ07_000194"
     CDS             complement(83336..84604)
                     /locus_tag="IWJ07_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAP9331805.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(84835..85572)
                     /locus_tag="IWJ07_000195"
     CDS             complement(84835..85572)
                     /locus_tag="IWJ07_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9331806.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(85639..86208)
                     /locus_tag="IWJ07_000196"
     CDS             complement(85639..86208)
                     /locus_tag="IWJ07_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAP9331807.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            86336..87208
                     /locus_tag="IWJ07_000197"
     CDS             86336..87208
                     /locus_tag="IWJ07_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9331808.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            87550..88422
                     /locus_tag="IWJ07_000198"
     CDS             87550..88422
                     /locus_tag="IWJ07_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9331809.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            88419..90377
                     /locus_tag="IWJ07_000199"
     CDS             88419..90377
                     /locus_tag="IWJ07_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9331810.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(90709..91560)
                     /locus_tag="IWJ07_000200"
     CDS             complement(90709..91560)
                     /locus_tag="IWJ07_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9331811.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            91683..92150
                     /locus_tag="IWJ07_000201"
     CDS             91683..92150
                     /locus_tag="IWJ07_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAP9331812.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(92413..92895)
                     /locus_tag="IWJ07_000202"
     CDS             complement(92413..92895)
                     /locus_tag="IWJ07_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9331813.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(92995..93858)
                     /locus_tag="IWJ07_000203"
     CDS             complement(92995..93858)
                     /locus_tag="IWJ07_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9331814.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            94456..95331
                     /locus_tag="IWJ07_000204"
     CDS             94456..95331
                     /locus_tag="IWJ07_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9331815.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(95476..95628)
                     /locus_tag="IWJ07_000205"
                     /pseudo
     CDS             complement(95476..95628)
                     /locus_tag="IWJ07_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(95896..97227)
                     /locus_tag="IWJ07_000206"
     CDS             complement(95896..97227)
                     /locus_tag="IWJ07_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9331816.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
BASE COUNT        27401 a        20856 c        17399 g        31638 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gttttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc tcctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatgggat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttatact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaataa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtca atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggtcgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22021 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22081 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22141 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22201 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22261 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22321 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22381 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22441 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    22501 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    22561 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    22621 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg ttttgctaat
    22681 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    22741 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    22801 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    22861 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    22921 ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg taccatagca
    22981 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23041 caaaactgtt cgtttcagaa aatgatcatg aaaaagccgc gttaagtcta gcatttctta
    23101 agataacaac gattcttttt cgctttcgac ctaaaaataa aaaaagaaat tattcatcac
    23161 tgaataattt cttaaaggga aaaatgaata atacaaatat aatcatgttc aacagcagtg
    23221 taggtcctaa aaaccttgta ataaaatacg tgttatttac tactgctaat ttaaaaatca
    23281 actggaccac catcgtaaac aattcataac ccgtgacaaa gatgatcata ctgaaaaatt
    23341 cagtaaagat gttgacatgg ataacgtctt tcattacata catcaaccat acaattaaag
    23401 ggagagctac tgcatagata ccgataacgc caatatagta agcgtcatag atagccccga
    23461 gaacaatcgt ggtaatcagt aaataacgtt tttcaaacgc catactgcaa cataataatg
    23521 ccaatatcag aaagtgggca tttgacatat aggtgccttt cgaccactcc cctagcattc
    23581 ttgttaaatg accatctatc aacatcaaaa agaacaaaac gattggcaga tagtacttca
    23641 ttgtttcttt cttcaacatg gactaatcct ccgctagccg ttggacaatc gtcacaactg
    23701 acacgtcata catttctgca taaggtttca cataaacttc ccgatccagc ccataactat
    23761 ctggtttcgt tttaataacc gtaccgatcg gcaagttagc tggagaattc cctccaagac
    23821 cggatgtctg gacaacatcc ccttctttga tatccgtatc accagttaat tgggtcacca
    23881 ctaaggcatg gagcttttca tcatagtttt taagcaaacc aaacgcttcg ccattagccg
    23941 aagatacccg tactggaaaa tgattggagc tttcattaga ggatgacagt aattcgattt
    24001 tagacgaagc cgtattgacc tcgatcactc ggccaatcaa tcctttttgc gacatgactg
    24061 ccatatttgc ttcgataccg tctttagaac ccttgtctac gatcagcatg tcctgccaac
    24121 tgtcaggcga acgagtaatg acatttgccg ttactttttc gtaactagtc agcgtctgat
    24181 tcaactcaag ttcttttttc aacgcttcga tttctctctc gtaattcttg ttttgctgtg
    24241 aaacgtcatc atagctatcg attttttctt ttagccgctc attttcggca taagttgaaa
    24301 aaagattatg gacagaatct actccatttc gtacccaccg tactggggca gagattgttt
    24361 tatcaaccat tgcaacactg tcattaacga tggattggaa gaaattgggc tttccttcat
    24421 tcgctcgctg cgcagcggtc acacttagta tcgtcaccac gatgataaca ataaccaagg
    24481 taataataat atttttatta ggattgaact tcttcacaaa gacacctcga attaattaga
    24541 ctcttgtgtt caattttatc actaaatgcc gtgaaacaaa aggtaagttg ctcctcttaa
    24601 gctttttttt agcaaataat agattctgtc atttttagcc tgaaagacaa atttttttat
    24661 ttgtttatgg atcaatcttt gctttgaata actgcaatca taccatctga aaaagaaaat
    24721 tctgcggtat ctgaaagaaa ttcgttactt gcataagatg tcggatattc tacttcttta
    24781 ttttccaata catacttgct gccttttaat gtaagattcg agacgggagt caagcagcaa
    24841 taagctaaat acttcatatc tggtatctgt cttactatat gctctccagg caaataataa
    24901 gacaaataat tttgacaatc tctgataata agctgatggg caaagggttt gaagcgaggt
    24961 tccataccta accagagatt ggctaaaaga tgatccaatc ttccgcccgt agctccaatc
    25021 aaagtgactt ccgcttgcgg gtaacgttca aatgttttta agatacctaa ctgtgtatct
    25081 gtatcgtctt tttctgcagg ggaacgggta tactctttgg cttttgcaaa tacttccttc
    25141 tgctcttcgt tcgacaatga atcaaaatca cccactgcca gatctactgg gtagcctgct
    25201 tgaagcaggt acagcgcacc acggtctatc ccgacatagg catcgtattt ggacagcaga
    25261 gactgcggcc agttttgcgg attacctcca gcagctaata aaattttcat ttagtctaat
    25321 gcctctttta atcgttcgat ttgtccgact ggattttcgg ctccatagat atatgatcct
    25381 gcaacgaaga catccgcccc tgcatcttta cagatttttg ctgtttctgg aacgattccg
    25441 ccatctactt cgattgcata agtccaattg ttctctctac gtagatcagc gagttccgta
    25501 attttttcta ctgtttcttc aataaagctt tgtccgccga acccaggatt gacagtcatg
    25561 actagtactt gatcagccaa aggcaaaaca tgtttgatca tggagactgg tgttcctgga
    25621 ttgataacta ctccagcttt aactcctaag ctttttgtca tttgaagtgc ccgatgaata
    25681 tgaggagttg cttcgacgtg gacagtaatg atatctgctc cagcttttgc aaatgctgga
    25741 atgtagtttt ctggatcaac gatcatcaga tggacgtcta acggtaattt agtaacagga
    25801 cggattgcct gaacaatatt tggccctaat gtgatatttg ggacaaatcg gccatccatt
    25861 acatctacgt ggatgtaatc tgcgcctcct ttttccacta actcaatgtc tctttgtaaa
    25921 ttggcaaaat ctgcactcaa aatggatggt gctatcttca ttcttgtttc ctcctatttt
    25981 cgtttacctt ctttttttgc atacattggt tttctttttt cgatttcttg taagaattgc
    26041 aaataattgt cgtaacgtgt ctgtgcgata ccacctgttt ctactctatg ttttacttcg
    26101 cagccaggtt ctttagcatg catgcactct ctaaatttgc aatggacaga agcttctaca
    26161 aattcaggaa attgtctcgg cagttctcct gcttcaattg tcaaaaagtc aatcgagcta
    26221 aatcctggtg tatctgctac taacccgcca tagataggaa tcagttccac atgacgtgtt
    26281 gtatgccgtc ctcttccaag tgcatcagaa atctctcctg ttgctaaatt cagttccggt
    26341 gatatttgat tcagtagcgt cgattttcct gctccagact gacccataaa gaccgatagc
    26401 cgttctggga agtaacggat cagttctgtc gtatcacccg tatgattggg tacaatcacc
    26461 ggatagccta ttttagtata ggtttgcttg atctcttcga ttttttctgg gtcgtctgac
    26521 aagtcggtct ttgtcaaaaa aataatcggt tcgatcgact cgtattccaa ggtaactaga
    26581 aagcggtcaa gtagattata agaaaatgca ggctctacga tacttgtgac aatgattcca
    26641 agatcaacat ttgtgactgg tgggcgaact aattgatttt ttctcggcaa gatttccaga
    26701 agatagccgt ctgtttgatt ttcactctca aagatcacct gatctccgac aagcggcgtg
    26761 attttccgat tccggaaatt tcctcttcct cttgtttgat aggtctctcc gtttgcgtat
    26821 acatagtaaa aaccgctgat tgcttttctg atttgtcctt tgataagtgc cacctccgtt
    26881 tttgtttatt ttaccatacg aagaggaaag taggtgttat tccatgcaaa cttctcaaag
    26941 tatatggctt gaaaattgat caaaagagat agaaacacaa aaaagagaat gcaacaaatc
    27001 aatgtcgcat tcccttctga tatattttat tcagcactta ttttgaactt tctgaggtgc
    27061 tagctgatga acttgtggag ctggaagatt cttcttttga agagttgctt gtgctactgg
    27121 agctacttga gctgctagaa ctgtctgaag agtctttttc agaactagaa gacgctggat
    27181 ctggtccttt agagtaaatt acgttcacaa ccattccttt ggtaattgtc gttcctgccc
    27241 ctggtactgt acgaatcact ttatctttat ccaccgtgtt tgaatattcg gaagtctcat
    27301 gcccgatata ttctgcaccg acactagcta aataactttg tgcttcgctt tttgtatagc
    27361 cggagatatc attcaattta ggctctgttc cttggcttac gacaaaattg atgtaagttt
    27421 cttctgctac aacttcaccc gaggctggat cctgactgat gatcgaacct gatggtacag
    27481 tgtcgctata gtcataagta gcactaatat tattgatatt gaatccttgt gtaacaagcc
    27541 aattacgaac agtttcgtag ttttctccta catgactact caaagtcact gtttctggtc
    27601 ctttgctgac tgtgaaagta atcgtgtctg ttttaggatc gacttctgta tcttctgatg
    27661 gagattgcga aatgatcatt ccgctttcta cctctgaact gaattcctct tctttttgaa
    27721 tcaagtcagc agaaaaccct aattctttca aacttgcttc ggcatcttca taacttttac
    27781 cactgtaatc atctaatttg atttttttcg tgccgctact aatatacaga gtgacttcgc
    27841 gtttttgctt gactgtcgtt cccgctgccg ggtctgtctt cactacatta ccttcttcga
    27901 ttttgtcatc tgcaatttct tctgtttttg ttgctacttc taatttcgct tttgcgagtg
    27961 tttctctagc agaagcttca gaaagatcag aaacatcagg aacaacaact tctcctcgcc
    28021 cgccagaagc aaagaataca gctgttccga ttcctaaaac agcaagaata gctaatagga
    28081 tcagccacca ttttttctgt cctttctttt taggatcagg cagaacttct tcctcctctg
    28141 gtaaggtttc gttttctggc ttgtcttttg gcagaggcat agatttgaat gaatcaggca
    28201 ttggtgcatc ttccgtaagg ggtgtgataa cttttgtttc attatccatc acatgaggct
    28261 gccatttttc ttcgtttgca cgagctggtg ataatactgt agaaagatca tcagacattt
    28321 cctcggctga cttataacgg tctgcaggtt ctttagccgt tgcccgtaaa atcacgtttt
    28381 ccagtgcttg tggcacattt ggatcaagtg cttttataga tggaagatca tcttgaaaat
    28441 gttttaaagc aattgttaca gcagattcac catcaaacgg tacgctaccc gtcaacatct
    28501 cataaaggat gatccctaaa gcataaatat ctgactgctt cgttgccata cttcctctgg
    28561 cctgctcagg ggacagataa tgaacagatc ctagcatcgt attcgtctgc gtgatcgatg
    28621 tttcagataa agcgatagca atcccgaaat ccgtgatttt tacaacacct tcattatcga
    28681 tcagtacgtt ttggggtttc aaatctcggt ggatgatttg atggttatga gctaaagaaa
    28741 tagcagaaag gatctgctgc ataatattta ctgctgtttc ataaggaaca ggataatgtg
    28801 tttggatata tcgtttcaag tccattcctt tgacgtattc catcaccaga tactgcatgt
    28861 tgtcttcttc accaacatcg taaacactaa caatgtttgg atgaacgagt tctgttgctg
    28921 ctaaagcttc tctctgaaat cgtcgaatcg cagtctggtc attttgaaag tcaaatcgta
    28981 agactttaac tgctacatca cgatccaaga ttaaatcgtg cgccaaaaat acatttgcca
    29041 taccaccgct accgatattt ccagtgattt gataacggcc atttaatttt ttgcccaact
    29101 cgatcataca ctgtcctcct tatattcgat aagaagaacg gtgatattat ccgctccgcc
    29161 tgcttcattg gcacgatcaa tcagctcagt tactttatga gacaatgggc cttcttgatt
    29221 gatgatcgtt cctatttctt cttcagacag catgttcgtc aagccatctg aacataacag
    29281 cagtcgatct tttagttgcc aaagatgagt agaaacatct acttcgactg ttccaggcat
    29341 gccgacagaa cgtgttaaaa tatttttcct tggatggttc actgccattt cttcgctgat
    29401 ttctcctgat ttgactagtt cattgactaa agaatgatcc tccgtcaatt gcatgatttt
    29461 atcttcacga atcaaataag ctctgctatc cccaacattg gcaatcgtga accagtcatc
    29521 tgacagagcg gccgctacga ttgttgttcc cataccgtta tattccggct gttcttgtcc
    29581 acgctgataa atcagcatat tttcttcttg aatcgattga atgaaccatt gggcgatttt
    29641 ttcatcgtca acagcttctt gtttttccca agcttcgcct agattcgtta ccgccatctg
    29701 actggcaatg tctccggcac gatgaccacc catcccgtct gctaatacag caagcttgag
    29761 acctgaacgg ctggtaaata cactggcata gtcttgattt gtgttacgtc gtcttcctac
    29821 atctgattga tattcaatct gcattttttc acctcattac tttttgcgca aacaacaaat
    29881 gaaaaatcca tctgtgtaaa actgatgcgg atacaaagtc aacattttct cttcgattga
    29941 tgattgtagc ataggattgg tcaaaacatc aatcctctca aaatcagggt gtctttccaa
    30001 gaaagcagaa acaacgtctt ggttttcttc ttttaagatc gtacaagtgc tataagtcag
    30061 aataccggaa gactttaaag ttggtgcgca gctttctaaa atttccaatt ggatttttgg
    30121 taaattcgct aattcatccg gctgtttttt ataacgaata tctggttttc gccgaagcag
    30181 cccaagacct gaacaaggcg cgtctactaa aatccgatca aactgctctg cttcgaactg
    30241 tttactgact tctctagcat ccatcttttg agcgtagaca gcagaagaga cacctagacg
    30301 tgctgcatta tcttcaatga gtttgacttt gtgttcgtgt acatctaaag cgaccacttc
    30361 tccaccgcat gacggatcta aaaaagaagc aatatgcgtc gtcttacctc cgggtgctgc
    30421 acaggcatct aagacatgat gttcaggctc gatttgcata gacggcgcta caagcatcga
    30481 actttcatct tggatcgtca tgacgccatc gtggaacaat gagctagaag ctaaatgccc
    30541 ttttttcgct actataccgt atggactgac ttggcttttt tcagctgtga tctcttcttc
    30601 ttgcaaaaca gtgatggcct gatctctgga aagtcggcgt gtatctactc gggcactagc
    30661 atggctaggc tgatatagcg ataaaccgag cttttcagtt tcttcgtatc cgatttgatc
    30721 caccagtcgt ttcgtcatcc atagaggcag gctgattttg acagaaagtc tttcgatagg
    30781 atcagcaatg ccttgcagat ctggtgctcc ttgacgctga taattcctca agacaccatt
    30841 tacaaatttc cctgtcccag gatttccttt tacttttgcg atttcgaccg cctcgtttaa
    30901 aatagcatgt gcagggactt tatctaaaaa ttctaattgg tatacagaaa gataaagaag
    30961 catcttgacc cagtcatcca ctttttttgc tttagcgatc aatggtgcaa cgtaaaattc
    31021 caatagtcgc tgatggctaa tcgtaccata gatcaattct gtaaataaac ccacatcttt
    31081 ttgatttagc tcgctttgtt tgatcatctc attcaacaaa agattcgagt aggcgccacc
    31141 cttgttcact cgctctaatg tcagtaatgc catatatcgt actgattttt tcattttttt
    31201 agggattttt ttcattattt cacctgctga ccaatctgaa cttgctgacc gcttccgtta
    31261 aggaaatcat gtacggcctg ttttccttta ccagctggct gtaattcatt gatggctaag
    31321 acggtctgtt taccgcaagc aatccataga ttttttttgt ctttccggat gatcgtacca
    31381 ggtgctttgt ctgtagtttc tgccaatact tcaaccgata ataatttcca gcgtatgccc
    31441 tcataggtcg tgaatgcaat tggccaagga cgcattccgc gtacttggtt gtcaatttct
    31501 tctgcagttt tatgccaatc gattgcttct tgttcccgtg tgatgtttgg cgaaaaagtt
    31561 gcttctgact cgtcttgagg tactggtttt tgtccatcca aaatattggg caatgtttct
    31621 agcaatagct ttcttcctaa aaggctaagc ttttcaaaca tcgtccctac atcatcttgt
    31681 ttagtgattg gtatgctttc ttgcgcatag attccgcctg catccatttt tttgatcatt
    31741 tccatgatcg ttacaccagt ttctttctcc ccattcatga tggcataatg aacaggcgca
    31801 cctccccgat atttaggaag taaagaagca tggacattga ttgcccctaa ttttggtact
    31861 tgcaataact tttcaggcaa gaattggcca aatgccgcag tgacgatcac atcaggcacc
    31921 aattcaatga tttcttccat ttctggcgaa ccggagattt tctctggctg cagcacacgg
    31981 ataccgtgct tcagagccgc tttttttacc ggtgtcggcg taattactcg tttccgccca
    32041 actggacggt cgggctgtgt taccactgcc tggatctcgt aacctgattc tatcagccct
    32101 tcaagaatcg gcacagaaaa agcaggtgtg cccataaata ctagcttagt catcaagatg
    32161 ttcctccata tattcttcaa gttcttcttc cgggatctgc tgtatcattt tctcaatgaa
    32221 caagataccg tctaagtgat cgatttcatg ctggaaggca cgggctaaat agccaaaagc
    32281 cgtcacttct atctcttctc cgtctcgatc ataataacgc acggtcactt catctgctcg
    32341 cttgaccgtt ccatagacat gcggaatact gagacaacct tctacgtcaa ggctttcacc
    32401 ttttgcttcg atgatttctg ggttaatcag ttcaaatttc tcgccttcat ctacttcgat
    32461 cacagcaatt ctcttgtttt gtcctacttg aggagcagcg atccctattc cgtcattcgc
    32521 aatcattgtc tcatataaat tgtctaataa agcgattgtc tcgtccgtga ttacatcgat
    32581 tggctgagca gttcttttta atttatcatt agggtggatc aatataggat agcgcataat
    32641 ttggcccttt cttaaataaa gtttaatggt tcgttgtcaa ttgccacaaa aagaccttgt
    32701 cgctgttctt tctggcttcc attcaaaatt tcatccaaaa catgaggaag attcggttca
    32761 tgtttatatt tgatgattag ttgataataa tatcggtttt tcactctcaa aatcatgctg
    32821 ggcgttggcc ctaatagcaa gctttgaggg cttaacactt cttttagctg attggcaatc
    32881 tgaaagattt tctttgccgc tacttgttct tcaggatggc tggcggttat tttcacagta
    32941 aagtaataag gaggatagcc tccacgatgc cgtacatgca tttcctgctg ataaaactgc
    33001 tcataatttt gttttttagc caattctatg gcgtaatgtt gaggattgaa tgtttggatc
    33061 acgacttcgc ctgctttttc cgctcgccct gctcgtcctg aaacttgggt aagcaattgg
    33121 aatgtatgct cactagaacg aaaatccggt aaattcaggg aggtatcagc attcaacacc
    33181 ccaactaatg tcacttctgg aaagtccaaa cctttagcaa tcatttgtgt ccctaataat
    33241 atatctgctt cttttgcacc gaatttttgt aaaatctgtt cgtgtgcccc ttttcttcga
    33301 gtggtatcta catccatacg caaaatccta gcttgcggat aaagttctcg gagttcctct
    33361 tctacttttt gagtacctgt tccatagtag cggattttat tgccgccaca attaggacag
    33421 cgattgggaa taggttcttc atgtccgcaa taatggcagc gcattgaacg tgtatccata
    33481 tgcagtgtaa gagagatatc gcaattagga catggaagaa caaatccaca gtccctacac
    33541 atcacaaaag acgaatatcc tcgacgattg agcagtagga cagtttgttc ttttttgtct
    33601 aatcgatttt gtattttttc ctgcaaatta gcagaaaaag tagaggtccg atgattctga
    33661 aactcttcac gcatatctac gatctcaata gctggaagct gtgcagctgc tttggctcgg
    33721 tggtttaact gcaataactg ataaacgcct ttttgagctc gtgctcttga ttctaaagag
    33781 ggcgtcgcgc ttcctagaac tattgggcaa tgatgatatt tactgcgcca aattgctaga
    33841 tctctcgcat gataacgcgg tgtctcatct tgtttgtaac ttgattcatg ttcttcatcg
    33901 ataatgatca gcccgatatt tttcagcggg gcaaaaatag cagatcgtgc gcctactact
    33961 acttgcgctt cttcccgctc aatcttgcgc cattcatcat atttttcacc ttgggaaagt
    34021 ccgctatgta agaccgcaac tgattctccg aagcgtcctt tgaatcgctc gaccatttgc
    34081 ggagtcaatg cgatttcagg cactaacata atagccgttt tattttctga caaaacatca
    34141 gcgattgcct gaagatatac ttctgttttc ccgctgccag tgattccctc cagcaaaaag
    34201 acctgatctt tttgctgctg acctgctgtg atgatttgtt cgacagcatt tttttgttct
    34261 gcattcagtt ccaatgccgt cgtttggtca aatactcggt ctttataagg gtcgcgataa
    34321 cgttctgatt ctataaactc cagccagccc ttttcttttc cttgattcaa gacagcagta
    34381 ctgattcctt tatttttgaa ataagcaatt ggttcttctt ggctcagttc agataaacac
    34441 aaaagaagtt ctttttgttt cttggcattt tgccgtaatt ttgcccattc ttcttcgatt
    34501 tgcttttcag ttaacaaaga acggaccaaa cgaatcgttt ttactttatt tcgtgtatgg
    34561 acttcatatt tgatatctac taattgctct tggcgccagc gaatcaattg agaaaggctg
    34621 ccttcttctt ctgcttcctt ccaggaaatc acttctcgct gatcaaacaa tacctgaact
    34681 tctggtgtgt ctgccaaagg atagatcaat ttatcatatt ctgctttcat cacactcgga
    34741 agcatcgttt gtagacaagt gattttgaat gcgtaagtaa tttttttcat ataatcagct
    34801 aatgaaagta attcttcatt gaccaccgga gcaaggtcta gcaaacgaat aactgccttc
    34861 aaagatggat tagctgattc ttctgatttt tggatagcca taacaaatcc ttggacatga
    34921 cgattgccat taccaaaagg gacttctact cgcatcccga cttgcaaagc tgtttccatt
    34981 tcagagggaa ccaaataagt gaatggctga tcggtctgca tagtaggtac gtccacaatg
    35041 acttcagctg ttaatgtcat ggttcgtttc ctcctttttt tgtcttttct tatcaagcga
    35101 caaaaagttc cttgcctatt ttactagaga tcgatggatt tgtcttatct tctgccaaat
    35161 cctaacaaaa aattaacagc agataagaaa aaaaagaact gtgtcaaaaa gttcagacac
    35221 agccttttct accagacaat aagtggcgaa tgtttgaaaa ggaaacagct gggtttgctt
    35281 ttctttttag atgttcgctc tatttttctt tattacattt tttgctcttc acggatacgt
    35341 gattccaatt cgtgttgttc acgctctttt tgtgcttgac gttcctcttc ttccatcttc
    35401 aaacgttcac gttttaattc aggatgtgga tcattgacta catttcctgc atcgatttct
    35461 tctaaagctt gaccgacgct ttttactgaa tcaaaagaat ctagagtagg ctgtgcactt
    35521 gcatccaatt catgcgcgcg tttacttgct aaaatcacta gagaatattt tgaatttaca
    35581 cgatctaata gtgagtcaat tgaaggtttt aacatcataa gactacatct cctttaacat
    35641 tttaatatat ttaccgatta cgcgatctac tcgaaaatgt tcactggcaa taatgttttt
    35701 gattcgttca actgctagcg gcacttcatc atttacaaca gcatagtcgt aaagagccat
    35761 catttcgatt tcttcttttg caactttcat tcgtttttcg attacttcat ctgcatctgt
    35821 tcctcggcct acgatccgtg attttaattc cgctaaatct ggcggagtca aaaagatgaa
    35881 tacaccatcg ggcacttttt ctttgacttg ttttgctcct tggacttcaa tctccaagaa
    35941 aacatctttt ccttcatcca atgtcttgtt gacatatgac aaaggagttc cataataatt
    36001 cccgacatat tctgcgtatt ccagcatctc tccagcttcg atcattgatt caaactcttc
    36061 tttgcttcgg aagaaataat caacgccttc gacctcccct tcacgcattt tacgggtagt
    36121 cattgagatc gaatactgaa aatcattatc ctcactctca aaaattgctt ttcgtactgt
    36181 gccttttcct acaccggatg gaccagacaa cacgattaat aatccacgct ctgacatgac
    36241 gacgtccttt caactagtct ttttgtactt atcgtttatt ttgcactttt tttctgcaac
    36301 tttcaaggtt atctttaatt atagctagaa attggtaaaa aagctaccct gtaccaagag
    36361 gtcgtaaaaa agctgtcctg catcaagtaa taagaacagc caaacataaa cagcttctca
    36421 tgtttttcgt ttggttgggg ttattatcgc agatgcaagc ttttgaacgc cgtgtataag
    36481 aggtcgtgaa gaagccgatt agtttcgagt atgaagaaaa attttgtgaa aatagtttct
    36541 cacatttttg cataaatttg gcttaatacc gagaaaatgg tttttgaaca tcacttattc
    36601 aaaataaaaa aagattgtga cacgaatttt gtcacaacct ttaaatgtat tcctagagca
    36661 aagaagctaa gcgaagaaat ccaattctgt ttatttattg tatttctttg ttcagcatcc
    36721 ttgctaaaag cccatattgg acaaagtatt cactacaccc atgatctcat cttttcgaga
    36781 acgtgctttt tctgtcggat cttctacctc ttctgtgtca attcgaacaa taaatttccg
    36841 attcatcgtc acgtaagcac cgtcgttgat caaaataaac agttcgttct cattatagtc
    36901 tgcacaggca tctttccata cgtcaaagcc gtttttttca gaagggatat cccgtttaac
    36961 cacagttttt ccacttaatt cgtggaacgt tacgtcataa tatttcatta tctctacctc
    37021 tttcgtcatt caatattttg tacttgttct ctgatttttt ctaagatcgt cttagactgg
    37081 acgacttgat ttttaatttc gattgcattt gatttggaac cgatcgtgtt gatttcccga
    37141 ttcatttctt ggatcaggaa atctaattcc cgacctacag gacattgtgc ttcgataagt
    37201 tcatctagtt tttgtacatg gatcgccaga cgatccagtt cctcgtgaat atctccgcgt
    37261 tccaacaaga tggctagctc cgtcaacaac cgttgctcat cgactttcgc tccaagaaat
    37321 tcctccagct ttttttgata ccttatttga aattcctttt catagatttc tacaaaacta
    37381 tcgagttttt ttagtacttc tttgaattct tcagtattct ttttcaacac taaagctaaa
    37441 gcttgacctt ctttttctcg actcgcctca atagaatcaa gtgcgctttt caatgtatct
    37501 agagctaact gtgccaattc ttccatgctc tcttccggtt tttcttctac cgttacgaag
    37561 ttttcgtcca gcacgaagcg ttccgccatc cgactaattg acacttgatt ttgactaccg
    37621 tatcgttgtt gaattccgtc tgtcaattga gtgataagtg aatcaatcaa ttcccagttg
    37681 atgacgatct gtttttcgtt tttgccaata taagctagat tcacaaatac ttcgattcga
    37741 ccgcggctta actgctgttt aataagctga cgaaaatcgt tttcaacaaa gttcaggatt
    37801 ttaggacttc gtatttgaac atctagaaaa cgctggttga cactttttat ttcaatatct
    37861 aactgatacg cttttgtttc gcggatggct ttgccaaaac cagtcatact tttcattcga
    37921 cagggtcctt cctcattaca tattttctat tgctttttgc agttgttcaa actcttcgtt
    37981 cgtcaatgtc aggcctttcc ccattttttc atggtctggc gcccaatctc gaagatcgaa
    38041 ttttggcggg cgaccattcc agctgactag gttcagttct ttacgccacc ccttgttgtt
    38101 ttctgacaat atagcgattt cttctacgat ttcatatgaa aattcttgtg ccatggttgt
    38161 tcctccttga gataatataa atataggatc agtaatgtgt caactggttg cttaagcaat
    38221 tcatgctgca aacgatcctt gcgttttcct tgtagcagat aatcaaaata aacagttttc
    38281 gtgcgctcat tttgtatttt ttgctggtat tttcgatacc ttcgccattc ttcagtactt
    38341 aattgaccaa tttgtgccaa taaatcatcc ggcatacttt ttatatcttg ttggcagatc
    38401 atttgaatca tcatgtctac ttgatagatt tcattgggca gtaaaaaatg tttttttgct
    38461 aaaagttcta gcatcgccaa tccgcatgtc tctggtacga ccctgttctt atataaatta
    38521 cgcagttttt gccaaaattt tatgcttttg aaaaaagata attctaactg aatttctttt
    38581 gttaagtaag acaaaaatcc tcgccagtat gttcttgcag gtttaacaga atgaaatgta
    38641 taccagttcc catagtaata gcctaatcgt tttttcattt ccagatagcc taattgtaaa
    38701 tttaattggt tcctttggct atcaaaaagc agaaagctgc ctagtgtcca caatgtttga
    38761 catttccaat ggataaaaat gtctggcata cgaatttttt tcgctgggcc tggcccttgc
    38821 acatctacga caaatgcctc tgttgctcct ttgttaatcg caatatcaat aggtattttg
    38881 ttgcgatacc caccgtctgc atatttattt tttccgatct ttcgataagc catagcggga
    38941 taaaatgaag cagaggcgag tatccaatct ggtatttctt ctgtttttgt ctgttggata
    39001 tcagtcacta cttccgctag ttctggcagt ttagtagaca ccgtatataa acgtattttg
    39061 ggattatgtt tgagtaagga caaatccaaa ttgtctttga ccatcttttc caatggagca
    39121 gaagaaaccc cgccttcaat tagtgctgtt ttcgtcattt ggcgtctttc tcgatagaag
    39181 cgttcacgaa ggtcttcaac tgcagccatt tctggtaacg caagcacttg tcttgtcgac
    39241 agctttttcc ataaattcac agcttttttc atatctcctt gtaaaatcaa tgcgccgttc
    39301 aacgcaccta ccgaggtacc tgtgataatc tcaaagttga tatcatgttc cttcagcgcc
    39361 tgccacacac caatctgata agcaccgtgc gctcctcctc cacctaaaac aagtgctgta
    39421 tgatagacca gctttttatt taattgtgtg gttccttgat agccgttttt atgaaaaata
    39481 cgcaaccatt cttctgatag aggagattga aataaaaaac aacagttttc tttaataaaa
    39541 aaacgagcgc aagcttccat ataccgaaaa aattttttcc attcaaaagt ttcaccttca
    39601 atcaacagat tattaaaaat aacatctcct ctacttttag caatagtaaa atagacggta
    39661 tattttttcg atactgcttt aaaagcacat tgcttttttg cagaacgcca aaaagcgtct
    39721 ctactttctt gatgactttg aaaaaatgga aggggttgtt ttgcttgctg gataggcgaa
    39781 tacagcgaca ttttctgtga aaagtaaact ttttttatct ccatcgcgct ctcctttttc
    39841 ttcaagtata ccagtacgtc ataaaaaggt catgagtctc gtattcgact ttgtagattc
    39901 cataagaaaa caattcataa aacctccttt aaataaaaaa atacaaaaat cacaaaaatt
    39961 aattgaaaat gagagtgttt tcaatgaaaa cgtgattttg taatggaatt ttaaaataaa
    40021 agaaacaagc ttttttaatg caaatgttgt aaaataaaag agattaagag gaaataagag
    40081 gaggaacatg atgactgagt cagtttatgt acatattgat accacaagta atgctgtttt
    40141 aacaaaaggg ctgactgcta ctgactttgc aaacagtatc gtgcattttc ctcaaaactt
    40201 gctcttgctt gatccatctg cttctgctgg agaatatgaa tcccatacag ggctgaaagt
    40261 aatccgtggt acagaaaata ttcagcgttt cttttcctct tctcgtaaca ggcgtggatt
    40321 cgatgaattg aagtggatcg actttactga tttaacgatg ctgaaagaat tgacaccttt
    40381 agaaatttca gaattattat attttggtca tatgaaaaca catcttcatt cgccgttttt
    40441 ttataaacta cagaataatt ttgtatactt cgatcttaat gatcagctga atcggattta
    40501 ttatcgctat ttagatgaat tttataggat ttttgctagt aaattaagtt cgatcttgtc
    40561 agataagatc aatgtaaaaa aatctttttt caaaaaagaa acacctgttg aaaaaatcag
    40621 tgtggaatta ttgaaagaaa tgcgtaattt gatgcaggaa gggatcattt tttcttttga
    40681 tcaagttggt ttgatcgatg gtaaatatcg tatcccaatc tatgtgattg aagattatat
    40741 ttggaaatta aaaggcaccc ggtatcgaga agaggaaatc atcgctactt taatctactc
    40801 tgtcaccgat cgacactggc aattggtaga agatgccgat ctttcttatc tgccattgaa
    40861 gtagatctat ttcttatcaa aaaataaaaa ggaaacaaga aactaggaca tgattctgtc
    40921 tcaagtttct tgcttctttt ttatttacag ctcctgtcgt ttatctggca caaaaatcga
    40981 aggagtcgtt tgttggatcc ctaataaaac gatgatcgtt acaatagctg tactcaaccc
    41041 gctggcacca ttcattacga aagaaaagag ccaaggattc atcccccata gtgcatacgc
    41101 tccccagaaa accactcctg caataaaatg ccaaaaatat ctggaaagtg cgccaatcaa
    41161 tgcaccaaac acgatgcttt ttctaatttg tttcgtatta tcgtctacat agcctttttg
    41221 gacatttgaa gccatgagcc ctgctgcgcc tgcaaaagta taggcaatcg ggtactcaat
    41281 caacacttga acgacactta aataataaac ttgtcccaac ggaaaatgta ggatgcccca
    41341 aatgaaacct gccagcatcc ctgcctttgt ccctcttctt aacgcaaaaa gagtcaatgg
    41401 aatctgccct aatgaaatag aaaaactgct gccgatctgg atcggaatca acgacaacac
    41461 catcgctaac gctgcaacaa tcgttccttc tacccaaact ctattttttc ccataaaaaa
    41521 acttcctcct ttgaataaaa aagtcacaaa ggaggaagca gctttcgaaa atagtcaaac
    41581 agctgtttat tcacaattcc tacgctcgca taaccgatac aggtacgaag ggtttagaaa
    41641 tagattcaat ctcagcccaa ttggactccc ctttgtgagt ttatttatta tttatttttt
    41701 gtttcaaaca atggactaac tggtttgttt tcatggatac gtttgattgc atctcccatt
    41761 agtccgccaa cacttacttc atcgattttg tcgattttac gatcttctgg aagatagatc
    41821 gagtctgtaa caactagacg ctcgatagct gaatcttcta ttctttgtaa agcagggccg
    41881 gataatactg gatgtgtaca tgaagcatac acactagttg caccagcttc tttcaaagca
    41941 ttggcagcca atgtgatcgt acctgctgta tcgatcatat catcgatcag tacacaagtt
    42001 ttgcctttga cttgtccaat gatattcatt acttctgcaa catttgcttt tggacggcgt
    42061 ttgtctatga tcgcaatcgg agatttcaaa aattccgcta atttacgtgc ccgtgtgaca
    42121 ccaccatggt ctggtgaaac aacgacaaca tcatcgcctt tgattccttt ttctaagaaa
    42181 taattggcaa taagtggcgc acccatcaaa tgatcgactg gaatatcaaa gaaaccttgg
    42241 atttggactg catgaaggtc caaagtcagc attcttgttg caccagcttt ttgaatcatg
    42301 tttgctacta atttcgctgt gattggttcc cgtgcacgtg ctttacggtc ttgacgcgca
    42361 tagccataat aaggcatgac tacgttgatt gtttttgcac ttgctcgttt cagtgcgtca
    42421 atcatgatca gcaactccat caagttatca ttgactgggc tgctcgttga ttgaataaca
    42481 tagacatgcg atccacgaat actttcttca atgtttactt gaatttctcc atcactaaat
    42541 tgagtaacag atgattttcc taactcgacg ccgactgctg ctgcaatttt ctctgctaat
    42601 gggcgattgg aatttaacgc aaaaattttt aatcttgggt caaagtaatg ttttgacatg
    42661 gggacctcca ctttcttttt cacaactcta gaattctacc taataaatag tagtcatatt
    42721 ttttaaataa atcaagtgat tttgcaaaag tcttagttta gataagggag tttttttgca
    42781 tatccttctt tattgacttg tcttgctctc gcaattgcca gcgcatattc tggaatattg
    42841 tctgtaattg ttgacccggc agcaatcgaa ctgtttttag ctacctcgac aggtccgatg
    42901 atattcgtgc tagaaccaat aaagctgtgg tcgccgacag tcgtatgatg tttgttcttt
    42961 ccatcatagt tgacgaagac aacaccacat ccgacattga tttcttctcc cagcgtagca
    43021 tcaccaacat aagtcaagtg accgacttta gttcctttgc cgatttgagc atttttcact
    43081 tccacaaagt ttccgatatg gacgttttcg ccaatttcag cttttggtcg taaatgtgca
    43141 taaggtccca catctgcatg tttcttgaca tggctgcttt caatgactga gttttcaatg
    43201 acgacgtgat cctcaatcct actgtctacg atttttgaat gtgacccgat gacacaatca
    43261 gaaccaatga ctgtatttcc ttggatttgt acacctgctt cgatcaatgt gtctggaccg
    43321 atttctacgc ctgcatcgat gtatgtcgtt gctgaatcga caaagcttac gccattgacc
    43381 atatgcatct ggttaattcg ctttctcatg atctcatttg ctttagctaa cgcgatccga
    43441 tcattgactc ccattgattc ttcaaaatca tccgtttgat aagcagcaac tgtatggcct
    43501 tcttctttta agatttcgat gatatctgtt agatagtatt caccttgagc attgtttgtt
    43561 ccaactttgc ttaacgcatc gaacaaagct tcgttgtcaa aacagtaagt tcctgtattg
    43621 atttcttgta caagagcttc ttctggtgtt gcatcttttt gttccacgat tttttctacg
    43681 atcccaatat ggtcacggat gattcgtcca tatcctgttg ggttttctgc ttgtgccgtc
    43741 aagatcgttg cacttgcatt ttttccttgg tgatattcaa aaagattgtt cagcgtttca
    43801 gtagtcaata atggtgtatc gccactgata actaacgttg ttcctttttt tccttccaag
    43861 aaagaagcag cttgcaaaac agcatgtcct gttcccaatt gttctgcttg taacgcatat
    43921 ttactgcgtt ctcctagttg tgctttaact tgttctgctc cgtgtccaac gatcgtaatc
    43981 acttcatctg gtttcgtttc actgacccga ttaatgatat gttctaccat aggtttacca
    44041 gaaacgggat gtaaaacttt atataatttt gacttcatgc gggtaccttt ccctgccgcc
    44101 aaaataattg cataacgtgc gtccaactgc gtcactccta aatatagttt gctgatttca
    44161 gtttatccca gtatacccat tttttcaaca aaacaagtga ttttctttta tcccagcgtt
    44221 ttctggataa aaaaatagat agaccataac gggcctatct atttggtaac cggtgctctc
    44281 ctgccaactc ctcggtcaca agtcacacta ctcataaaac atgaaaagct gttttttgaa
    44341 atcgtttctt gtgacttaga gttaaacggt aggcttacaa cctcttcatt aaacgttttt
    44401 caatcagctg accttcggta ttagctcaat aaacggcaaa atatgaggag caatttttcc
    44461 gtttattgag ctaatactca ggacaggacg ctgatttcac aacctctaga taaacggtgt
    44521 tcaaaagcca gtttctcggt attaagccaa atttatgaaa aaatgtggaa agccattttc
    44581 acaaaatttt tcttactact cgaaactaag cggctttttc acaacctctt attctgaaaa
    44641 ataatttcct ggcacaaccg taatattttt cgtttgagta tctacatctt tgacataaag
    44701 cagtgacgta taatcatcga tggcgcgatg accgttgaat tttgattcgg caaatacagt
    44761 gatgccaaca agttctgatt cgaattcttc aatcaagctc ttcattccgt tgactgttcc
    44821 gccgcctttc atgaaatcat ccactacaag gacacgtgaa ccacgtttca aacttctctt
    44881 agacagttcc atcttttcaa ttctttcaga agatccagac acatagttga cacttactgt
    44941 tgatccttct gttatttttg agtcccggcg tacaataacg aatggcgcat tcagataata
    45001 ggacacagct tgggcaatag gtacgccttt tgtggctaca gtcattacag catcgatttt
    45061 ttcaccaagg tatttagaag caatgattct tccaacttga cgcagcagtt caggttctcc
    45121 taacaaatca gagagataaa catatcctcc tggaagcaga cggtcttgtt ctgatagacg
    45181 ttctgctaga gattcaatat attttttcgc ctcttcatat gaaatttcag ggatgaacaa
    45241 tacaccacct gcagcacccg ggattgtttc caaggttcca taccctcgtt ctttaaatgt
    45301 tttcttgatg attcccaaat cttcactgat agaagattta gcagattcat aacgttcggc
    45361 aaagcttgtt aaggaaatca attcatgcgg gtgattcaat aaatactgtg tcatgtcaac
    45421 tagacgttca ctgcgacgta ctttcaccta ttccaactcc ttttaagaaa ttttatcgtt
    45481 ttacattata taagtttttc ttcaaaaatt cgagcatttt atgaaaaaat aagaaaatag
    45541 ttcggtattt attaaaataa aaagaagcct ataaaaaatt tttcgttttt ataaggcttc
    45601 ttttctttat aatttagtta ttttttcttc tgaaggcaac caatattcgt ttgatgatat
    45661 ttacaatcag gaacaaagca ataaagacta atgtgattgt tgctcctgga ggtgtatcta
    45721 gataaaacga gctagttagt cctgacagca tccctagaaa tcctacacaa acactgatag
    45781 caatcacgac attgaagctt ttgcctaatc gcataccgat tgctgcggga agaaccatga
    45841 ttgcggatat caaaagtgca ccggcaatcg ggatcatcac agcaatagcc acaccagtaa
    45901 tcacattgaa catcatcgac atcaaatgaa cgggtagccc atccacgtgg gcggtatctt
    45961 catcaaaagt cagaacatac atcggccgtt tgaataaaaa gaacaatata ccgatggcaa
    46021 cgaataagat tcctaaaaag atgacttgct cccatgtaat ggtcacgatc gaaccaaaca
    46081 gataggattg gatacttgtt gctgaattcc ctccgctcaa attcatcaat actaacgcta
    46141 gcgccaaacc accagccatt aaaatggcaa tggagatttc tgaataggtt cggtagatcg
    46201 ttcgcagata ctccaatatc acagctgcta atacaacgac tagcatggtc gtaagtgtag
    46261 gattaaggtt caaaaagaat cctaacgcta cccctgctaa agagacatga gaaagcgtat
    46321 cagccatcag cgattgtctt ctgatcacta aaaacacacc tagcatcggg gcgattccgg
    46381 caatgaagat agcggcaaga aacgctcttc tcatgaattc atatgaaagc aacgccattg
    46441 agaatcctcc tttctcacca gtcggatctg tctgtccgca tactgtttga tatcttcatg
    46501 atcatgggtg atcattagaa ctgatttccc atggacatgc gcactatgtc ggagaagacg
    46561 ataaaattca tttctggatt cttcatccat acctgttgtt ggttcatcaa gaacaaataa
    46621 gtcagggtca gtagcaaaaa tacgcgctaa actgattcgc tgcttttgtc cgccagacaa
    46681 ttcacctatt cgccgatttc gcatatccca catccctaca gcttctaaag cacgctgtac
    46741 atgttgatga tctttttcag agagacgctt gaaccagcgg cctcttggat atcttcctga
    46801 acggactaat tcgatcaccg tactaggaaa acctgcattg aaagatgcaa cttgttgagg
    46861 aatataaccg atacttaatt tttttccttc ttgatttttc tcagcaatct ttatcattcc
    46921 ttttgaaggt ttcagtaatc ctaacgatgc tttcaccaag gttgacttgg ctgctccatt
    46981 ttctccagtt aaaatgacaa attcacccgc atctacatga taagagacat cttctagtac
    47041 aggttcatca tcatagtaaa aggtcaaatc agatatttcg atataacgca ccttacaacc
    47101 tccattctaa aacttaattg atactttctt ttaaagcttt cagatttttt tccatgacag
    47161 aaatataatt ttcgcctgct ttgatttgtt cattagtcaa gctttctaaa ggattcaaca
    47221 cttctagctt cactcctgtt tcattcgaca atgtctcagc taccttcgaa gaggcatttt
    47281 cttcaaaata aatcactttg atgttgtttt tcttcacaaa ttcttttagc tcagctaagc
    47341 gacttggtgt gggttcctga tctggtgaaa cgcccgaaat cgctacttgt tttaagtcgt
    47401 attgattcgc taaataagca aaagcagcat gctgagtgac aaatgtgcgg tttgttgcat
    47461 cttttagctc atcggagtat ttttgatcca gtgcttcaag ctttttgata taagcatcgg
    47521 cattcttcgt aaaagtttcc tttttatctg gatatttttc gcctaattct tcagcaatcg
    47581 ttctgacttc ttttatagct aataccggat cgagccaaac atgtggatcg tattcatgtg
    47641 aatgaccatg cccttcttct gattcttcat gttcgtctgc ttcttgcgat tctaaacggt
    47701 cgattccttt agctgcttcg atcatcaagg tctgtttgct atccactgaa tcctttaacg
    47761 aatccacaaa aaattccatg tctgaactat tatacacgaa aacatctgca tcggaaattt
    47821 ctgctctttc ttttgcactt ggttcgaaat catgcggctc cgttccagca gggatcaaaa
    47881 gtttcacgtt tccttcatcc cctacgatct cttttgtaaa atcatacatc ggataaaaag
    47941 tcgtaacgat cgtcaaatcc tcttttttgt ctgcttcctt gttcgtattt ccacatgctg
    48001 caaacagtaa agcacctatc attaccgtag ctgtcaaaag atattttttc atttatatgc
    48061 tcctttcaat gaacaaatcg gaattttacg atttattaac gtgaactaat gtatcaaaat
    48121 aaaaaaacac agtcaataga aatccatcaa tttctgattt aagcatgtat attcttgatt
    48181 aaatcgtaaa atggatgagt tgctgctcat atctagccat ttcactacaa taaaaaactg
    48241 aagaatacca ttttaggatt cctcagtttt tcatcaccta ttttacttct tatattttat
    48301 tgcagggttc gtactaaata aacttcctca caaaaacctt ttaacccatt atatactctc
    48361 tgcgctcttg aataatgttg acacaaggca aaaacggttg gtccgcttcc actcatcaat
    48421 gcagcatctg ctccgtactt gatcatacgc tctttgattt gatggatgac tggatgccgc
    48481 tttcccgtta tatcttctaa actgtttccc atttttttga tcatctgctg ataatcttgc
    48541 tctcggatgg cttcagcaag tccgtggatg tcttgatgtt ggatggcttg cgtatctact
    48601 tctctaaaca ctgtacgtgt agagacactc attcttggtt tgaccaacac aacccagcac
    48661 tgtggcatag aaggtaatac ttggatttct tcccctttgc caccgacaaa tgccgtactt
    48721 ccgtagacac agtaaggaac atctgtaccg acttccaaac cgatttctgc cagttcttct
    48781 aacgaacagc ccagattcca taattggttg atccctctta gagcagctgc acagtctgta
    48841 cttcctccgc ctaatccagc tgctactgga atattcttct taacaaagat cttgacaccg
    48901 gttttgatat tgtatttatt ttttagcaat aaagcagctt gataaacatt gttccgttca
    48961 tctaccggta aaaatgcttt atttgtttct acaatgattt tgtcttctgt tatttcttca
    49021 aaaaatagat gatctgccaa gtctacgctc gacatcacca tttccaactc atgataccca
    49081 tcttgccgtc tatataatac atccagtcct aaatttatct tggctggcgc tttttcaatc
    49141 aattccatcc tctcacccat tcgaaaagtc taagtcgcaa gtattctatc atagggaaaa
    49201 ataaaagtat agcttatgtc tgcctagtgg ttgattttgt catcgtttca acagctttca
    49261 cagaattttc tcttcataat ccaaacaagt tctagtgatt cttataattc tgtgagcact
    49321 tcttctgcca aactcggaac atttcctttt tcttttccta tcagactgag tagctgcatg
    49381 gtagcaaatc cgaaattact tcctttccta taagcaaaat aacgtccttc ccaatgatcg
    49441 acatatttta atttcttttc ataagcagac tggaaggaat agaaaaaatt accataatta
    49501 tatacgatat gcatgacttt ttcttgaaag aaagaaaaat ctgtttctcc aacattagct
    49561 aatggagcta agcctagatt ggcttccgag tagcccattt tctcgtatac aataaacaga
    49621 ttggctaaca gatagtcagc taattcctca gattctttag gtaagacccg caacaaatca
    49681 tatcccgcct gcttcttcgt catcggctga gcggtgatga agccaacgac tttttgatct
    49741 ttacggacaa taccgacacc gctagataat acatatgatt tatcaaatcg tcccacagag
    49801 aagtattttt ctcgtgcacc ttgaagccat ttttgtgaaa cttcatttac agcttgatgt
    49861 aattcttccg gcagttgatc gtaccaagta aaatggtatc cttctttcat caaacgttga
    49921 tattcaagtt gctgactcag ccgagacaag tcttctgttg ctagatcaac aatccctgct
    49981 tcacctactt tcgtgaagtg aaaacccaat tcatgaagca tcactacgta gctttctgat
    50041 atcttataaa acgcaagctg atagcctaaa caatctgctt gtttcataaa ctgattcgtt
    50101 gctgcccgca tcattttagg attgccaact ggatctccta aaacaaagca tttgtttcct
    50161 ttgatctgat aaccaaaaac gacttgatct tggtcgtctt cttggtaata aaaataactg
    50221 taacctgaca agcgaagctg atggcttgta gctgtgcctc cgtatgtttc caataaacgg
    50281 tcaaaacggt ctttttcgta ctcactgcca agcttcgtag tatcttcagc taaatattga
    50341 taaagcgcca ctaaagctag caatgagatg cccaaaccaa taagccctga gaaccacaca
    50401 tcatctgaag gaaacaaaat aaaatgattt cctgttattc gaccgactaa aaagcgtccg
    50461 ctgtgatagc ctgcaattgc gtaaacgata aacaaaaagc cgaacaagct agcatcaaag
    50521 attatagctc cccaagaata aacgaatttt gttcgataaa attcttttct ggataaccac
    50581 acagctaaca gaatcaagac atataccaat atcaactgcc aagataacgt acgtgtgatt
    50641 gtatttaaaa taccaaaaga tagtaaagca atcgtaggca gaaatgcctt tttgactttt
    50701 actgaaagtg ctctagcaag gccgagtaat aaaaagccaa ccaatagatt cactgtctga
    50761 tctaaaaaat tgaatgagaa cggcaataat acttgaaata gccgactgat tgcagacaga
    50821 ttagtgattg ttgatagaag taccatcatg atccccgcaa aatagacagc acttacgagc
    50881 acgatatgcg caaccttttg gaaaaacagt cgcggaatgt tatctaaaaa tcgattgatt
    50941 ttgactcctg tatgtgtgat gaacaataga agtcccgtca taaaaggtag aacataataa
    51001 aacaaccgat aatataatag ccaaactact gctacatctt gccgaacaga caattgactc
    51061 attcctaaga tcatcaaaac gtcaaaagtc cccattccac ctgggaccat cgtcagcatt
    51121 cctatcaatg tagcaatcat gaacatagga tagatcgaaa gcatcgaaat atctattctc
    51181 atcaacttgc cgatgatcaa aaataccgca agggctcccg tccattgtcc aaaggatgcc
    51241 agtagcagac caagcaagcc ttttggataa aactcgaaga acaatgtctt tctctttaga
    51301 taagcaaaaa agaaaatgac tggtgctaaa aaactaccgc cgaaaagcca gatccaatag
    51361 cttcgaaaaa tactttctga ctgaataaaa aagacatcaa taaatgtaag aaacgaccat
    51421 acagacaaac cagtcagcat aaatagtgcg acttttgata cagtggctaa aaccattttt
    51481 ctatttgtct gctttccata aaaactagcc cgcagacttg ctcctaccac accaccaaaa
    51541 ccggcgagat tgttgatcgt gtttgttacc cacgcagcaa gaagaaactc ttttctttcc
    51601 atttttggtt ttccttgttt ttctaagact ctgatcgtca cccaatcata caacaacatc
    51661 ggagtgacac ccagcagtcc agccagaatc atgagcagga tcgtcatgcg gctttgttgt
    51721 cccatccttt gccaaatatc ttgccaactc atgccattag caatatttgc gacttgattt
    51781 gccacaaaga ctaacacgga gccgaaaaaa atcagtttta aaatcagact atgttccttt
    51841 atccattgca gcatcaagta tcgtttattt ttcacatttt cccattccta acttgtatac
    51901 ttttgtgtca taccccaatg ttatcttatt tattttaatt tttccacttt agacctgttc
    51961 tttgcttgcg atgatagaaa aagaaagata tcctaatgct aaaggagagg ataacatgaa
    52021 agtaggattt atcggcacag ggaatatggc acaagctatt attgatggtt tattaaaaaa
    52081 aggaacagtc tcgaaagaag aggtgttcgt ttccagtgga cattttgaaa atgcacaaaa
    52141 atttgctgct gaaactggcg tccatgcatg caaaagtaat caggaagttg catcacaatc
    52201 agatatcgtt cttttagccg tgaaaccaaa aatcattgaa aaagtattgg aagaactaag
    52261 agaatatagc cagaaagtct tattcatttc tattgcttca ggaaaatcat taatagattt
    52321 agaaacagcg cttgaagatc ctcaggcctc catcgtacgt gtgatgccga atgtcaatgt
    52381 cagcgtagga gcaggcgttt ctgcgatttg caaaaatgaa gctgccacgg aggaacagct
    52441 tcagcaagct acgatgattt ttgaagcaat cggcagcgtc tatcctttag cagaatcaga
    52501 tttcagtaca ttcattggcc ttgctggcag ctcacccgca ttcgtttaca tgctgatcga
    52561 tgcgatggga cggactggtg tactgaatgg tttgtccaaa cagacagcca ctgagattgc
    52621 tgcaaaagca attatgggtt cttgtcaaaa attcctttca agtgataaaa atccatggga
    52681 acttgtagac caagtttctt ctcctggcgg tacaactgtt gcaggtgtag ttgccttaga
    52741 agaagcagga ttcatcccag caatcatcaa aggaatcaat acgacgatcg agaaagatct
    52801 tgagatgttg aataaaaaat aatgatagca atgttttttc gtgccttaca aaaaaagaag
    52861 agctgtggct tttgccgcgg ctcttctttt gtatttttta ttatttagca taatcagttg
    52921 cacgagtttc ccgaataacg attactttga tgtgtccagg ataatccagt tcttcttcga
    52981 ttcgtttacg aatatcacgt actaaacgaa cagcgtctaa atccgagatt tcttctggct
    53041 tgaccattac ccgaacttcg cgtccagctt gaactgcgta acttgattct acaccggcga
    53101 agctgttcga aatattttcc agacttctta aacgacggat gtagttttct aatgattcac
    53161 ttcgcgcacc aggtcttgct gctgagagcg catctgctgc agctacaagg acagagataa
    53221 cagaagtagc ttccacatct ccatgatgag aagcgatcgc gttgattact actgggtttt
    53281 ctttgtactt ggcagctaat tccgcaccga tctcaacgtg agatccttca atctcatggt
    53341 caagtgcttt cccaatatca tggagcaacc cggcacgttt tgctaattgg atatcttcgc
    53401 caagctctgc tgctaagata ccagctaatt ttgcaacctc gaccgaatgc ttcaagacgt
    53461 tttgtccata actagttctg aatcgcagac gtccgatgat cttgattaga tctggatgaa
    53521 gtgtatgagc accgacttca aacgctgcct cttccccgta ttcgcgaata cgttcatcca
    53581 tttctttacg agatttctcg accatttctt cgatacgcgc tggatggatt cgtccatctt
    53641 gtatcagttt ttccaaggtc atccgtgcga tttctcttcg gattggatcg aatccggaaa
    53701 gaacaactgc ttccggtgta tcatcgatga ttaaatcgat accagtcagc gtctcaagcg
    53761 ttcgaatatt acgaccttct cgtccaatga tacgaccttt catttcatca ttcggaaggg
    53821 taacgactga aacagtcgtt tctgaaactt gatctgctgc gcagcgttga atcgctaatg
    53881 ataataagtt tttcgctttg cgatctgctt cttctttggc acgctgttca gattctttta
    53941 ccattagagt taactcatga ttcagctctt cttcagtcga tttcatgatg acatctcgtg
    54001 cgtcctcttt tgataaacca gcaatttttt ctagttcttt ttgttgatct tcaatcattt
    54061 tttcaacgtc tttttctcgc tcttcaatta attgctgttt tgaactaagt gcttcttctt
    54121 tactttcaag tgagttctcg cgtttttcta acgagtcatc tttacgattc agcgtttgtt
    54181 cgcgttgaat caaacgattc tcttgagatt ttaattctaa cttactttct ctcagctcac
    54241 tttcaacttc agaacggtat ttctgatttt cttccttagc ttctaacaat gcttcttttt
    54301 ttagagtttc tgcttctttt tttgcactat cgatgattcc agcagctgat gattgtgcac
    54361 cagctatttc tttctcgtga cgtgactttg cgaccataaa ccctaaacca agaccgacaa
    54421 ttaaaccgat gatagcgagg agaatgttga ataccataaa atccacctcc atactatctt
    54481 tttcattttt atcatctata cagcttctat gataaattat tctaaactcg tcaatatgca
    54541 tcttcgcata tatgtgtaca aaaaacatgc acaactttat tctaatgttt gtaccccgta
    54601 gtgtcaactt taaataaaca aagaaacggc tttctactta ttcattaaac gatggttttt
    54661 aaaataaatg acactagttt catataggta aacctttaaa ttgagaaact tttctattaa
    54721 attattcttt gtagatgaac agatattttt attttatgta cccactcgta taattaaata
    54781 actcacgatt tctttattca ataaaaaaac ggcaggtcaa ggagagtacc tactcttcag
    54841 accagccgtt tttgtttatc ataattttta ttcttctact aaaggcaatt cttcttgtcc
    54901 tttttcatct tctgggacag cgacttcttc accaatacca taagctgctc taactagagc
    54961 agaaacttct gccatcattt caggatgatt agccatatag attttagcgt tttcccgtcc
    55021 ttgaccaatc cgatcttctt tataagaata ccaagcaccg cttttatcaa caatgtcttt
    55081 ttctactgcc atgtctagaa gttctccttc ttgtgagatc cctaaaccat acatgacatc
    55141 tacttcagcg attttgaaag gaggtgctac tttgtttttt acgactttga tctttgtacg
    55201 gttaccaaca atatctgtac cttgtttcaa ttgttctgca cgacgtactt ccaaacggat
    55261 cgttgcgtag aatttcaatg cgcgtcctcc aggagtgatt tctggattcc caaacatcac
    55321 gcctactttt tcacggattt ggttgatgaa aatagcgatt gtttttgttt tgttgatcga
    55381 accagagagt ttacgcaatg cttgagacat caaacgtgct tgtaacccga catgtgagtc
    55441 acccatttcg ccgtcgattt ctgctcgtgg aactaaagca gcaactgaat cgactactac
    55501 gatatctaca gccccacttg atactaaagc atcagcgatc tctagacctt gttctcctgt
    55561 gtcaggctgt gaaagaagta gttcatcgat attcacacct aatttttgtg catattgcgg
    55621 atctaacgca tgctcagcat caatgaaagc ggccgttccg ccattttttt gtacttctgc
    55681 aatagcgtgt agtgcaactg ttgttttacc tgaactttca ggtccatata cttcgatgat
    55741 acgtccacgg gggtaaccgc cgacacctaa agcgacatct aacgcaagag agccacttgg
    55801 aattgtagag atttgttggt cgattttttc gcccaatttc ataatagagc ctttaccata
    55861 gctcttttca atttttttca gtgcagcatc taaggctgct ttacgatcat ctgccaatac
    55921 gataatcctc cttatattta caaatcagag caacatttca ttatccttat catatctttt
    55981 ttagtgaaaa aaagcaagaa aaaaccgaac aaatattcgc atttatcatt ttcttaaaag
    56041 ctgttttctc aaaagatcaa atccttgcat aaccgcatta tatctgatag cagcacgatc
    56101 tctggcaaag tgatattttt ccgaaaccac gccgtcttta gatgaaatag cgatccatac
    56161 tgttcctgca gattgacctt ctaacgtgtc cggtccagcc actccggtga aagctacact
    56221 gtaatctgtt cttgctatct cacgtgcttg aatggccatc tgctccacac attctttgct
    56281 gactgttcca tattctgcta atagctccgg atcaatctcc agaaaaccag cttttgtttg
    56341 gagcgagtag gtaacgaatc cgcctggaaa tacttcagat acaccagcaa tgtcgcctaa
    56401 tgcggcttgg aaggcgccgg ctgtcaaact ttccgctgct gttacagtct gcttgttttc
    56461 tttcagtagt tccactacga cttttgctag cgaattatca tcgccgtatc cataaaaata
    56521 ttcccctact cgttcttgga ttttttcttc taatgctaaa agagcttgat tccctgcctg
    56581 aacatcattt gttttcactg tcagtcgtaa tgtcacttca tttggtttgg catatggtgc
    56641 gatcgttgga ttgatctgtg tctcaatcag atcttttagc tcagtgacca gtcgcgattc
    56701 accaatccca taaaagcgca aaactttaga aattagtttt tcttcagaag gaaaacgttc
    56761 ctctagtaaa ggacgtactt gttccacaaa cattggtttc aactcattag gcggaccggg
    56821 aagaaggata tacgcatgtg tatctgtttg ataaaaagtc cctaaagcaa gccctgtacg
    56881 atttggtaga ggaaccccgc cttcgataat ctgggactgt tgcagattgt ttttggtcat
    56941 ttttcgatgg gttgtttcaa aatatgccaa taattttttg tatccttccg tattttgtat
    57001 gagagactta ccgaggtgag ccgcagttgc ttcttttgtc aggtcatctt ctgtcggtcc
    57061 taatccgcca cataagagta tgagttcact tcttgtttca gctagagcaa tcaactcttc
    57121 taaacgctgc tgattatctc ctacaactgt ttgataataa acttcgatac ctagatcagc
    57181 tagctgttct gacaagaaag tggcattcgt attcacgact tgtcctagta aaagttctgt
    57241 tccgacagct attatttctg ctttcattac ttcccgctcc cttatatagt atacgctgta
    57301 tttttatatt tttttattta aaaagagggc aaggagctgt gacaaaaatc ttgtcacagc
    57361 tccttgttcc aaataaacag tgttctcctg ccaaaacctc ggtcataagt cacaatatac
    57421 ataaaacatg aaaagctgtt ttttgaaatt atcccttgtg actcagagtt agacggcagg
    57481 ctcacaacct ctccactaaa cgatacttac attgatcctt tgaacacacc gctgtttttg
    57541 atgaagtaat ccacacctga atagatagta aagatcagac aagcataaag catgatttga
    57601 tctactggaa taccgatcag gctgaatggg atattattga taaacagcaa gataatagca
    57661 accatttgtg tagccgtctt gacttttccc ggccaagctg cagccatgac ttctccatgt
    57721 tcgacaagta gtaaacgcaa gcctgtgacg gctaattcac gacagacaat gatcgctact
    57781 acccaaccag gggcctttcc ttggtcaacc aacatgataa aagcagtcat aactagcatt
    57841 ttatctgcca acgggtccgc aaacttgcca aaattagtga caaggcctcg cgcacgggca
    57901 atttttccat ctaaccagtc tgtgatacta gctaaagcaa aaataactgc acctactagc
    57961 tgtgtgacag ccagtgtggt gtctcctacg ttaaattgtc cccaatctaa aggtaaacct
    58021 acaaccaaaa tgaaaatcgg gatcatacaa attctaagta cagttaattt atttggtaaa
    58081 ttcacactat cgactccttt cttccctatt ctacgtgact atcagcgaag tttccaccga
    58141 tattatttac ttttccaaat gaatttattc aaaggagtat ttttattgaa attgatcttt
    58201 ttgaattaac gaagaaggac cgcgacacag atttgtcacg atccttcttt tttataccta
    58261 caagtaaaca gatgcgtaaa ctaatttata cgatcattct gcgtattgaa gagtaaggtt
    58321 cacatttttt ggactctgat tgttttcccc tggattaact ggtacggatt ctccattcgc
    58381 tttgatcgtt gcattgcttg ctgcaccaat cgtgatggtc gcatttgttg ctgtttccgg
    58441 taacacggta ctctgtgttt catttgctgc cagtgtccct tgataaacat aggcattgtc
    58501 tactagcaca cctaaccata cacgattgac agcagtaaat tcaagggtta caggcttggt
    58561 agcgtctgtg atatttactg cgatggcact gccagaatct tctcctgtag ctattgccat
    58621 tttcttttct tcacttgatt ttgttgttga ttctactgta gaagactctt tggtctgcgt
    58681 gctttgctct gaggaagagg actcctcgac tgatccttct actttaagcg aagaagctgt
    58741 gtctccgatt attggatcag cttgacgatc ttgccaagtc atatagccca cgacaacgac
    58801 aatcgccaaa gcaaccaatc ctaataaaat gacaggaaga cttgtccaaa agcgacttgg
    58861 atgcgattct tcctgatgca ttgcttttct agaagtactt actgtttcag gctgcggacg
    58921 ttcagggacc gatcctgtca atttgctttt cccatcaaaa acatcgacta aatggtctcc
    58981 atcttctcct acagcttcag cgtattgccg aataaatgcc cgcacgtaaa atttaccagg
    59041 caattggtca aaatcatcat tgtcgattgc ttccaaataa cgtttttgaa tttttgtaat
    59101 ctgctgtaat tcatctaaag atattttttt attcaggcgt gcttgccgta atttttctcc
    59161 gatggtttca aaagccactc gttcatcttc tccagtctgt ttgattctcc gaagcatctg
    59221 ctgttgcaac gaaagacgtt atgtttgggt gctgtgacaa aagtcatgtc acagctcctt
    59281 ttttccgaat aaacattgtt ctcctgccaa ctcctcggtc acaagtcaca atatccaata
    59341 aatataagaa gctgtttttt gaacttgttc cttgtgactc ggagttaaac ggcaggctca
    59401 cgacctcttt cttaaacggt gttcaatcag ctgttcctcg gtattagctc aaaaaccggc
    59461 aaaatataag gagatatttt tccgtatgtt tctctaatac tcaggtcaaa acgctgattt
    59521 cacaacctct tccttaaacg gtgttcaaaa gcttgcatct gcggtaataa gtccaaccaa
    59581 gttaaaaaca ttaagaagct gtttttactt ggctgttctt attacttgat gcagggcagc
    59641 tttttcacaa cctcttggta cacggtgttc cagaagtagc ttcttcggca ataagccgaa
    59701 atatcacaaa aatttgagaa gcaattttcg taaattcctt cttatttctt gaagctgacc
    59761 acttctgtca caacctcttt gattacttcg tttgatttta ttttaatata agttgattaa
    59821 aagagagcat gatttgtttt attttctcac taatttttcc aacacaacta agaataccat
    59881 aaaagctgat tttggaggag gttcctacta caagtctaaa acgaaattta agacttttcc
    59941 tcataaaata ctggtttatt ttggtctcat gtaaaactcg ctgaaggcct cttcacttat
    60001 aaatgcttcc ccagctgcca gtacgtcttt catttggatc gaatcgatga tttcaggaag
    60061 atcaaataaa gttcgatcgc caaataaact ttgcgtaaat tgattggcaa tatattcgat
    60121 cgaatttaat gattggaaat attgtccaag cattttcttc ttcagcaaat cgagatttgt
    60181 ttctgacact tccggatcat cagcgaatcc aagaatgatt tctttgactt tttctgctgc
    60241 tttctctggt tcgtccgtat ctccgctgaa gtccgcaaag tgaaattcac gatccaagct
    60301 gaattcaaaa ccaaagctgt catcgataat tccttgattg tacatcgcta gatagtttcg
    60361 agaagtattg cctaaaatca tttggaacaa cagattgatt gctgttttat agcgtaacag
    60421 ttctgttcct tcttgcggca atgtgtctaa tcctttaata ccaagtacaa atttatctcg
    60481 tgtaatggct gcttccaatg cactttgttt gatgatttct tttgtgtttt cagggaaata
    60541 acgtacaatt tcttgctttg gagggaaatt ttttgcttct tggttttccc gaataagttt
    60601 catcaatttc tcaggctcca tcttaccaac tacaaataaa accatattgc ttggctgata
    60661 aaacgtccga tagcacgtat acaagtcttg agctgtgatt ttgtcgatac tttcaaccgt
    60721 gcccgcaata tcgatatgca atgggtgtgt aggatacaag ttatttagta tgccaaaaaa
    60781 catccgccag ttagggtcgt cttcatacat ttggatttcc tgaccgatga ttcctttttc
    60841 cttgtttaca gtttcttctg tgaagtaagg tgcttgtaca aaatcgatca gagtcgtcag
    60901 attcttttct acctgatccg tcgtagaaaa cagatagctg gttttcgtaa aacttgtgaa
    60961 agcattggcg gaagctcctt gtttcccaaa taattgaaag acatcaccat cttctttttc
    61021 aaacaactta tgttctaaaa aatgggcaat tccatctgga actttcacct tttctttttc
    61081 accataggga ataaattcgt tatcgatcga accataattc gtactgaaca aaccatatgt
    61141 cttgtgatag tcatttttag gcaacaaata cacggtcaaa ccgtttggca gtacttcatg
    61201 ataaagtgtt tcattgattt gctcatattc tgttttattc atttgcattt cctcctgcta
    61261 aaaagaagat tgcctgcaat tcgatttgct cagctacttg ttgtatctct ttgatcgtta
    61321 cgctctccat acgtttcaac cattcttcat cagataattt tgtttccggc agccagctgt
    61381 ccaaaaaaga agcttcgatt gccgcttgcg gagagtctag agaaagcaga tattggtttc
    61441 tcagcattgc ttttgtctga gacagttcca actctgttat ttcaccattt cgcaaacttt
    61501 ctaattgctc gtgaatcaga tgaagtactt gatttcggtt cttttcatcg ataccggttt
    61561 ggacactcat gaatcctcgg aacgtatcca cgctactaga ggcataataa gctagacttt
    61621 ctttctctct gacattcatg aaaagtttag agtgaggaaa accgccaaat aaaccgttaa
    61681 agaccattag cgcaaaacgt tctggttcat cgtaatacac atttgtctga taagctaggt
    61741 tcagctttgc ttgaacgatt ggctcacgta cttgtcgttc cttcacgata ttcacttgag
    61801 gttgggtata aaacagatct ggataaactg tttcccgcga ctcaaatggt aattgcccaa
    61861 ctgcttcagc tgctttgtcc ggatcaacat cgccgacaac aaaaatgtcg atttgatcat
    61921 ttgccatcat ttcttgatag taggacgcta gcgaactagc agtgatcttg tccaaatctt
    61981 cgactacgcc caaccctggg atcttttgtt ccgggctgtt ttcaaaataa agttcttgca
    62041 aggcatagga agcaaaagtt tgtttatctt ccttcaaact ttccaaatat agacgcagat
    62101 tgttcttttc caagtcaaac gtcaactgat caaactgatg atttttaata tttggataaa
    62161 ataaaacctc ttttaaaaaa tctaccgctt gagaaaaaag gtttggatca ttgacatatt
    62221 caccattcac aaaactgatc cctgcgttta gccaatgtaa atttcctttt tttctaactg
    62281 ataatccaaa gcttgcgcca taaagctctg caagttgcgc acttaacttt gtctgatccg
    62341 aataattcaa actatttgtt tccaacatgc tgctgagaag tgaacgtttc gtgatgactt
    62401 cttgttgcag acgcgcagta aaacggataa aaatccggat cgttttgtat tgttttgttg
    62461 gtaagacatg aagattcacg ccttctgcca attgatacac catatttata tgctcctttt
    62521 cacagttgat tgcccgctta ctcttttaat gggtcaacct gcctttttta cttattcctc
    62581 tgcttgaaat ttataagttc aggcaattcg ccgttttttc taaatgatgt tacactttat
    62641 agtatagcat agatgttttt tatcaaaaga aacagacggc taaaagcgta tattgttttt
    62701 ttatttttta tttttcgata tactaaagac aaataaaaga agggagggtc attcatgatc
    62761 aaagaattca aagagttcat catgcgtgga gatgtcttgg atcttgctgt cggcgttgtt
    62821 attggtagtg cgtttacaag catcgtaaac caagtggtgc aaggcttgat cacaccacta
    62881 ataggttggt ttgtcgcaat gatcacaggt acttctgatt tagaaggagc actttcaatt
    62941 cttgactggt cgccgacaaa aggcgtcaca tttgcgtttg gtaacgttat tagtgctatc
    63001 attacatttc tgatcactgg ttttgtctta ttcttagttg tcaaagcggc aaaccgtgcg
    63061 aagaatttac gctccaaaga agagcaagtg gaagtggaag aaactcctac tgcagaagat
    63121 tatcttagtg acatacgcga tcttcttgcc aaacaagtgg cagaagacaa tacacatcca
    63181 gtagagcaaa caaatctttc cgatacttct aaataataaa gaatctaaaa aaaagaaagc
    63241 cgattttcag tgtcggcttt ctttttttag attcttaact tgctacttgt tcctttttca
    63301 tgtcgatatg tggaatatca tcttccaaat acacatctga gattgccttg aatccaaacg
    63361 actcataaaa agcttgtagg tattcttgtg cgccgatcac gattggtttt tctggaaaat
    63421 ttttctggat catgttaatc gtttcttgaa caatctttct tcctaaacca tctttacggt
    63481 tgtcctgatg aacaaggaca cgtccaaaat ggatactctc cgcttcttca tatactcgcg
    63541 tgtaggcaag gatcttccca tcagtatctt gaaaaaacgt atgccaagct tctccatcaa
    63601 tgtcatcaac ttcttgataa ggacactgtt gttccacaac aaaaactgcg attcgttctt
    63661 tgacgatcgc gtaaaactct gctgttgaca attcttcaaa tttctttacc tgatattcca
    63721 ctaagcaaac gccccctgct tttttattct tagcataccg attcgcacac aaaaaagcaa
    63781 acgaacctta cgaatctcag gctcgtttgc tttttaaact gcttcagtta aaaattcgat
    63841 ctcaactgtc cgagtaagta catctgaata gctgtaagat actcgttcaa aagaattttc
    63901 ttcaggatcc aaatcaacaa cgaatactga aggataggtt tctgttaaaa cgccgcggcg
    63961 ttctgtttga cgctttcttc ctgtctgagc aaccaacatg attgggctgc cgatacgacc
    64021 ttctaaatct ttcttgattg aagctaaagt tgttggcata cagttcacct ctactatcaa
    64081 ctataccaca tatttttaaa attttcaagt ttaccataaa aaaaaaagaa atgcaataaa
    64141 aataaataag aagctcatta agatttttct aaattattcc tcattataaa atttcaagtg
    64201 tttttttatt ttgagtttta ctctcccgta agcgctcatt acttgcttgt gatccattgc
    64261 taaacattca gccatgtcta caaaatcaac gccttgcata taataatgaa aaattttagc
    64321 ttctattttt gaaaactgga tttcgcaatt ttcgattgct tcttttaata aaagatgctg
    64381 aattgctaaa ggttcttgca atgtgatctc tgctaaaaag tctggtcctt ctgtctctat
    64441 tttttggtcg attgagactt catctttttg atcacgacgc ttcagcgcgt tctgcttcct
    64501 caacagagaa tagatttgat tgcttataac gcttttataa taacttccaa agctaacacc
    64561 tttgcttttt tcgtaagttc tcaatgcctg atataaagcg atccttgctt cttgttccca
    64621 atcctcagaa acataatttc ttaaataata ggtgtttttc aaacgtagta aaagtggaaa
    64681 atatttttga tacagggcag caaatgcttc ttcgtcacca ttacgtgcag cgcgcaataa
    64741 ctcctcagac ataaaataat tcctccataa attcgtttat ggaggaatct tatcatcaat
    64801 gattcatcga actgattaga acgatatgtt atttattttt tctcattaaa tcgtccaatt
    64861 tttgggacaa tttttttaac tgctcttcat tccatggaga attacgtcgg aaatcaataa
    64921 aatgcatatc tttattttct tgggctattg ttttttccgt tttttttatc gtcttatata
    64981 attcataagc agaagttcgt aatgctcctt gggaaaagat catccactgt tctgctaaat
    65041 cgcttgtcgc cactgtcact tgtgtcaaac ggtcattcag ctgaccagcg attctctcga
    65101 tataactgtc agccgtttcg tcttctttcg taaaaacaac atccaactgg tatttcttat
    65161 aattttgttg gatgcccgga actagttggg catcgaagac aacgatgact tccattcctt
    65221 catatttcgc ataatttgat aaacgatgta aaagagcttc tcttgcatcc gccattttgt
    65281 cctgcttttt aagctggaca agttccggcc acgaaccaat catgttgtag ccgtctacaa
    65341 tcaacagctg ttttttcatg gtctactccc cctgacgttg ggaaaaagcc tgatacataa
    65401 gcaaaccagc agcaacactg gcatttaagc tctggacgtg cccggtcatc gggatcgtca
    65461 gcaattcatc catttctttt ttcaatcctg gactcattcc tttgccttca ttaccaatga
    65521 ctaaagcaat tgatcctttg gcattccatt tttggaagtt tgtgccggtc atatctgtgc
    65581 caaataccca aaatcctgtt tctttgagct gttgcacagc ttgtaccaaa ttcgtcacgc
    65641 gtgcaacagg gacatgttcg actgccccag tggaagtctt tgttacgata ggtgtgattc
    65701 caactgctcg gtgttttggt atgatcacac catcgacgcc acttgcgtct gctgtccgta
    65761 aaatcgaccc aaaattgtgt ggatcttcga tgctatccaa aatcaaaaag aaggggtctt
    65821 cttttttctc ttttgttcgt tcaatcagtt ggtcaaggga taaatattca taaggtgtaa
    65881 tcgcaagaac gactccttga tgcacgccgt gatcgctcaa agtttctaat ttttgtttag
    65941 gaacccattt gactggcaca gcttgttcac gtgcgatttc tttcagttct tctacttttt
    66001 ctcctcgtga atcttcttga agaaagagtt tattccctcg cccttgttgt agcgcttcta
    66061 ctgcggcatg gtgcccaaag acgaaattat cttctaatgc ttcttcattt tgatcagaaa
    66121 ctgactgatt tcgtccagtt tgctttttat ttgaatatct attttgtttt ttgtcttcaa
    66181 atccacgttt cgattgatgt ttgtttgttc tttctttacc catttttttc accaactttc
    66241 tgaatacacc agcggatcaa ttcttccatc cgctcggttt gttttgtcaa atgcagatag
    66301 cccatgagcg cttcgaaccc tgttgcgacc cgatacgtcg taacatcagc attttttgcg
    66361 ctcgtatggc ttttagcatt tcttcctctg cgataataaa gttcttcttc ttctgtcaaa
    66421 agatgttctt ccagcatttg ctgaatcaaa aaggcttgtg cattcgcaga aacataattc
    66481 gttgccgcac gatgcagctt attgggtttt gtttctcctt gatcaatcag gtggtcacga
    66541 acatagattt cgtaaatcgc atctccaaca taagccaagg ctaaaccgtt caattgtgtg
    66601 taatctctca tgcttctctt ctccatcggg ttccttgcgg tgtatcttct aataagatcc
    66661 ctttttcttt caacaaatca cgaatttcgt cgcttcggac aaaatcacga ttgcttcttg
    66721 cttgatttcg ttcttcgatc aattgttcaa tctcttcatc taataactct tctgctgtaa
    66781 ataaaatacc gaagatcgaa agccattgcg agaataaagc ttctccttgt gccaagacag
    66841 cggcagaaac tttcggctgg tctagataag tgttcagcca cttagcaagc tcatagacca
    66901 ctgtgatccc atttgctgca ttaaaatcat cgtccatttc ttcgatgaat cgctgttcta
    66961 aattttctaa caaagcgatt ttatccgcat catctgccaa attctctgag gcagtttctt
    67021 ggcggaattt caaattgtca aacgtattct tcagtcgttg taaattggtg gctgcttctt
    67081 tcatcgtcgt ttcactgtag cggatcggac gacggtattg tgtggtcgcc atgaagaaac
    67141 gcaagacttg tggatcaacg tttttgatca tttcatgtac agtgatgaaa ttccctagag
    67201 atttactcat tttttcatcg tcttcgccaa tcgttacata gccattgtgc atccagtaat
    67261 tagcgaattt tttccctgtt ttcgcttcac tttgtgcgat ttcattttca tgatgaggaa
    67321 attctaaatc ttgcccgcca ccatggatat cgatcgtttc tcctaaatgt tttgttgcca
    67381 taacggaaca ttcaatatgc caaccgggac gtcctttgcc ccatggtgaa tcccaagaaa
    67441 tctcgccagg ttttgcttgt ttccataaag cgaaatctaa tgggtcttct ttttgctgct
    67501 gttcttcccc tgtacgctga cttgctccga cttccagttc atcgatggat tggtcactta
    67561 atttcccata attttcaaat ttacgggtac ggtaatacac atcacctgaa gattcataag
    67621 catagccttt ttcaaccaac acttgaataa atgctaaaat atctggcata tgatccatga
    67681 ctcttggatg cagtgtagca ggttcgacat tcaacgcttt cgtatcttct tcaaatgctc
    67741 ggatgaaacg atcagcaact tctggtgctg taatacctaa ttcgtttgct gtacggatga
    67801 ttttatcgtc tacatccgtg aaattcgata cgaaatcgac tttatagcct cgatattcaa
    67861 aatagcgtcg aattgtatcg aaagcaatgg tgctacgagc atttccgata tggatataat
    67921 tatacacagt cggtccgcag acatacatac cgacatgtcc cttttgtata gggatgaatt
    67981 cttctttttt tctggtcaat gtattgtaga tttttatcat tctgatctcc tcactttttc
    68041 tcctttgatt cgaacgactt ttgctggtac tcctaccgct gtagcgtcgt ctggaacatc
    68101 tttcaacaca acggcacctg caccgatttt ggcgtttttt ccgatcgtta ccggtcctaa
    68161 tatctgcgca ttcgcagaga gcatggctcc ttgtttaaca gttgggtgtc ttttacccgt
    68221 ttcttttcct gtgccgccta atgttacgcc atggaataaa atcacatcgt cctcgatttc
    68281 tgcggtttcg ccaatgacga ttcccattcc atggtcgatg aacacgccag ttccaatcgt
    68341 tgctcctgga tggatctcaa caccagttag gaaacgccag aattgagcat tgatcttcgc
    68401 caataaaaag agccggtgct gatataaaaa atgagagaaa cgatgccaaa acaaggcatg
    68461 aaaaccaggg tatgtcaaaa cgatctcagc tgtcgaacgt gcagccggat cgtttctctt
    68521 tgctgcatca atcgctcggg taaaccaact catgactttc cactcctttc gtaagaattt
    68581 tatgtaggaa atcatcttct tataaagaaa agaagcgcct tttaaataag tttccttatc
    68641 aaaagacgct ttccgcgtgg ttccactttt cttcataatc aatgattatc tctgacagct
    68701 aacgcctgtt atacgttctt ggctactttg tttcctataa aacagttttt cctgttttct
    68761 gtgtttcgcc aagaccactc ccagaggcat ttcggcaacc attcattgat accttgcacc
    68821 acccggtatc tctcttgtaa tgatcagctg cttacttttt tctgttcatc gtgtttagtt
    68881 cagtacttga tttaaatgtg ctaatgattt ttcacgacca agcaattcga tcgtttcagg
    68941 caattctgga ccatgcattt gtcctgatac agctacacgg attggcatga aaagattttt
    69001 tccttttacg ccagtttctt tttgaacagc tttgatggca gctttgacag ttggagcatc
    69061 tactacttcc atttgttcta gctgtgcttt gaatgcgtgg agaacagtag gaactgtttc
    69121 accagcaagt acttcttttg ctgcttcatt caatacagga tgttcattga aaaatagcgt
    69181 agacagatca acgatttctg cagcatagct catttgaggt tgatacaggc taacgatctt
    69241 cttcacccat tcgattgtct tttcatctgg gttttctgct acacgtccat cagcgattag
    69301 ataaggcaga cacatgtcag taaatgcatc tagatccatt gctttgatgt aatggttgtt
    69361 tacccattgc agttttttcg catcgaaagc tgcgggtgat ttacttaggc gttttggatc
    69421 gaacattttg atcaattctt ggtgttcgaa gatttcgtct tcgcccacag gtgaccagcc
    69481 caataatgca ataaagttga acattgcatc tggtagatag cctaattcgc gatattgttc
    69541 gataaattgc aagattgatt cgtcacgttt gcttagtttt ttacctgttt cactgttgat
    69601 gatcaatgtc atatgtccga aggttggtgg tgtccagcca aatgcttcat agatcatcag
    69661 ttgttttggt gtattggcaa tatggtcatc tccacgtaaa acgtgagtga ttttcatcaa
    69721 atgatcatcg accgcaacag caaaattata agttggcata ccatcgcgtt tttggatgac
    69781 aaagtcgcca ccgatattat cggattcgaa cgaaatagcg cctttcacca tatctgtaaa
    69841 tgcgtattct gtattgcgag gcacacggaa acgtacaaca gattctagac cttgtgcttc
    69901 tttttctgct tgttcttcag gtgtcaagtt cgcacatgtt cctgcgtaat gaggcatttc
    69961 tcctcttgcg cgttgtgctt cccgttctgc ttctaattct tcttctgtgc aatagcattt
    70021 gtatgctcga ttgctagcaa gcagctgatc aatcagcggt tgatagattt ctttacgttc
    70081 agattgacgg taagggccgt attctccagg attttcagga gattcatccc aattcattcc
    70141 caaccaagca aggttttcca actggctttt ttcgccatct tcgatattac gtttttggtc
    70201 agtatcctca atacgaataa taaagtctcc atcattgtgg cgggcaaaca aataattgaa
    70261 taatgcagtt cttgcatttc caatatgcag gtgcccagtt ggacttggtg catagcgtac
    70321 gcgtactttc gtcattttct tcctcttctt tcaaactaga ctttgactaa cagcaaaaat
    70381 tccgtcagtt acggcttctc aaatatcatt tatttaaagc cactcagcac aaattttacc
    70441 ttttctaagg taaatagtaa agccaactgg ttgaatttcc ttaccagttg gctgaatttc
    70501 ttttttattt tgattgttta gtctttttct ctgtctcttt tttattgtcg attcctcgtc
    70561 ctgcatgtgc aggtttagca aagatcatgc gtccagcagc tgtttgaagg gcacttgtta
    70621 caacgacttc gatacgttca ttcatgtaat gctgtccatc ttctacgaca accatcgtgc
    70681 cgtcatcaag gtaggctact ccttgttggc gttctgtccc ggcttttact actagaacat
    70741 tcatcgtttc accagggatg acgactggtt ttacggcatt tgccaacgca ttgatattaa
    70801 gaacaggtac attttggaat tcagagactt tattcaagtt atagtcattg gttacaatga
    70861 caccatctaa aagcttagcc aatttgatca atttgctgtc tacttcactg atatcttcaa
    70921 agtctccatc gtacatttcg acagatatac cctcttcttt ttgcaaggca tttaggatat
    70981 ctaatccgcg acgaccacgc acacgtttca aactatcccc tgagtccgcg atgtattgta
    71041 gttcgtaaag aacaaaatta gggatcatca aggttccttc taagaatcca gtctttgtaa
    71101 tatcataaat ccgaccgtca atgatcacgc ttgtatccaa gattttatat ttatggaaat
    71161 tttctcctga ttttcgttct aacagttgac tctcaacttg actagctgtt tcttgctcga
    71221 tttttttgct tcgtgaacca aaaactttct tccattcttc gattcgtgtc gttcccattc
    71281 tcaatcccag atagcctaag atgatcatca gtaaaatcgg caatacacta ttgacgaatg
    71341 gaatttccat attgtaaaga ggggtcgaga tcacaacacc taaagtcaat ccgatgatcg
    71401 caccgatcgc accgaagaga agataagtca agctgacctc acttagtttt tgttctactt
    71461 gtttgataat agctgagata tgtttggcca acagcaaaga taataaaaag aaaataagtg
    71521 cgcctaacag gctgttggta aatgtattat tcagccatcc gtttgactgt tgaccgattg
    71581 cctgccaagc tgagggtaaa aatgaaatac ccaagcttgc gccgacaatg atcatcagca
    71641 aagtaatgac acgtttttgc atcgtttttc ctccgtttcg acaatcattt attagttagc
    71701 ggaacacacg cttcaacgtt tcagccaaag ttgctactcc aactatctcg attccttttg
    71761 gcgcttccca gttacctaag ttgtttttag gtaggtatac ttttgtgaag ccgagttttt
    71821 ggacttcttt gactcgttgt tcaatatgac tgacacgtct gatttctcca gtcagtccaa
    71881 tttcaccgat gaaacattcc gttggctgtg ttcctttttc tttatagctt gaagcaatac
    71941 tgacagcgat agctaaatca atcgctggtt catttaattt taccccacct gcagctttta
    72001 agtaagcatc ttgattctga agaagcaacc cagctctttt ttccagcact gccatgatca
    72061 gagatacccg attgaaatca agacctgttg ttgttcgttt ggcatttccg aacatagtag
    72121 gtgtgactaa tgcttggatc tctaccaaaa ttggacgtgt cccttccatt gccacaacga
    72181 tagcagagcc agttgcaccg tctaggcgtt cttctaagaa aatctgtgaa ggattagcga
    72241 cttcttccaa tccatgctca tgcatttcaa aaatcccaat ctcattcgtt gagccaaatc
    72301 ggttttttac tgctcgcaaa atccgaaaac tatgatgttg ttcaccttca aaatataaca
    72361 ccgtatccac catatgttct aacattcttg ggccggcgat cgagccttct ttggtaacat
    72421 gaccgacgat aaaaatggct atcccatttg ttttagcggt tttgagtagt tctgcagtgg
    72481 tttctcttac ttggctgaca cttcctgcca cacttgtgat gtctggctgc gtcatcgttt
    72541 ggatggaatc aatgataaca taatcagggg agatatgttc gatggcacga ctgatttcgt
    72601 tcatatctgt ttcagcgtat agataaaatt ctgtgttgat acttccgagg cgttccgcgc
    72661 gtaattttat ctgctctgcg ctttcttcgc cggatacata taagactttt ccgcctatgg
    72721 ctgctaattg ctgagaaact tgtaacagca aagtggattt cccgatacct ggatcgccac
    72781 caatcaagac catagaccct ggcaccacac ctccgcctaa cacacggttc agttcttcta
    72841 attttgtttt gatccgaggt tcttttttag ggacgacgtc tgcgattttt gttggttttg
    72901 ctttttctcc tgtcaaactt gtccgagttc tgcggtccgt tgtatcttgt tcgatttctt
    72961 cgaccatcgt attccatttt ccgcaatttg ggcagcgtcc caagaactta ggcgacacat
    73021 acccgcaatt ttggcaaaca aattgtactt ttgatttttt tgccattgta ccgcctcctt
    73081 ttatcctaat catgagacat taaatcaagc ttaagtatct ttaacgtttt atttactgga
    73141 agaaccaaat ccgccttgac gttgagcagt cgtttgatct tgatctgctt ttaaaaacgg
    73201 caaaaagatc ccttggccga tacgctcgcc ttttttgatc actacatcct tgaaaccaaa
    73261 gttggtgaat tggaacatga tatggccttc atttgcctca ttattgtaat aatcactgtc
    73321 aatcacacct actccgtttg tcaacactaa aaaccgcttc aacggattgc ttgaacggtt
    73381 agcaagctgc aaaaattcat cttctcccat gtaagctttg attccagtag gcacgagggt
    73441 tggtttcaat tgtttttcat tttctgatac atcttttttc aatccatctg cgactgtttc
    73501 caaccccttt ttccagcctg ctttccagat acttggaatc acgacctctt ctgctgcttc
    73561 aaaatcatag ccagcagcat gatgtgtagc gcgctgcggt agattgatat tttgatcttt
    73621 atatttagaa ataacttcaa atcctctttt tctcaccggt tttcctccct aaataaacta
    73681 ctcctattct aacatatccg aggctgtaaa agactttctt tacaaattct tcaagaaatt
    73741 ctgctatctt aaagtaaacc attaattcgt tctttttcca aacgactgca agttgtacaa
    73801 aaaaactatt ttttacatga ctttactcgt caaaacaagt agaatagaac gaaattaatg
    73861 agtggatatt tgattgaggt gtcacatgcg taaagaaatt aaaaatgcaa aacgaattat
    73921 tgtcaaagta ggaacaagta cattgattta tcccaatgga aatatcaatc ttgcggcaat
    73981 cgatcagtta gcttttgtcc tttctgatct tagaaatcgg ggaaaggaag tgatcttagt
    74041 ctcatcagga gctgtaggcg ttggtatgca taaattacat ttgtcagaac gtccgacttt
    74101 gattcctgaa cagcaagctg cggcggctgt tggtcaagcc gaactgatga atatctataa
    74161 tcaaagattc agtgtatata atcaacagac ggcacagatc ctcgtgacaa gagacgtggt
    74221 agattatcct gaaagtcgta aaaatgtgag gaatacattg gaacagcttt tgattatgga
    74281 cacgatccct atcgtgaacg aaaacgatac tgtagcagtg gatgaattag atcatctgac
    74341 aaaatttggc gataatgatc agctgtcagc catcatggca aaacttgtcg cggcagacct
    74401 tttgatcgtc ctttctgata tcgacgggtt ttattcagat aatccgacta caaatcccga
    74461 ggctcagatg tatcgctata tcgataaaat cgatgaaaag attctggcac aagcaggtgg
    74521 tgctggtagc acatttggaa caggcggtat gcagagtaaa ttaaaagccg cttctaggat
    74581 atttgaagca aacagcgcaa tgattttagc aaatggaaaa aatccggcaa ttatttttga
    74641 tattcttgca ggaaaagaag taggtacttt atttaaggag gacttgaaat gactgacttg
    74701 atgcaattag gacaacaggc aaaagatagc gcgcaacaac tagcacttat gcctacaaac
    74761 cgcaaaaatg agctacttgt tcaaatggca caaaccatca aaaagaatca acaagcgatc
    74821 attgaagcaa atcataaaga cttagaaaaa gcagtcgaaa acaatatcag tgaaacgatg
    74881 caagaccgct tgcagctgac acctgagcga atcaatgcca tggctacaga gatcgaaaaa
    74941 attgcttcgt tagacgatcc tattggaaaa gtagatgaga tgtggacgaa caccgacgga
    75001 ttgagaatcg gcaaaaagcg cgtgccacta ggcgtgatcg gcatcattta cgaatctcgt
    75061 ccaaatgtca cgacagatgc agctagtcta gcattcaaat cagggaatgc cgttatcctt
    75121 cgcggtggca aagaagcatt cttctcgaat caattattag tccaattatt gcagcaagta
    75181 ctggtcagtg ctggcgaatc tccttatgcc atccagtttg tcgatgatac ttctcatgaa
    75241 actgcaggac agttgatgcg attgacagaa tacttagatg tgctgattcc tcgaggcgga
    75301 gcaaaattaa tccaacgagt caaagaacag gcaacggtac ctatcattga aacaggtaca
    75361 ggaaataatc acgtatatat cgataaagaa gcacagttat ctatggcagt gaacatcatc
    75421 gtcaatgcaa aagcttcccg tccatctgtc tgtaacgcgg cagaaacatt gctgatccat
    75481 tctgagatcg cacctttttt cttgccagct attgaaaaag aactggtaga acacggggtc
    75541 tctttacgtg cggatgctcg tgcgttagaa tatctagaga cggctgcttt agcagaagac
    75601 acagattggg atactgaata tctagattat atccttgctg tcaaagtggt cgattctttg
    75661 gatgaagcaa ttgcacatat caatcggcat aatactaaac attcagaaac gatcgtgaca
    75721 gatagttatg cggctagtca acgtttctta aatgaagtgg acgcggcggc tgtctatgtc
    75781 aatgcctcca ctcgttttac agacggatcg gtatttggtt ttggtgcaga gattggtatc
    75841 tctactcaaa agttgcatgc ccgtggtcca atggggttaa atgaattaac ttccacaaaa
    75901 tatatcattt atggcgatgg acaaatccgc gaataatgtt tcgtgtgaaa cattattccc
    75961 gccttttgcc ttttttctgt aaaggttcta tccaactaga aaggtgtcaa tatgtttgaa
    76021 attttaatga ttttttttat ttaccttatt tctctgaata tcgctgcttt tctgggagtc
    76081 ggtattttat cccttttctt tcaattcaaa aaacgaagca tagggagtca acgggaaaaa
    76141 tggtcgcagt attttgacaa aatcgggcaa aaagggctgg ttacacgctt gcacattagt
    76201 tacatggttg cattatgctt attagcgaca atcaattact attctttttt cgatcattca
    76261 atcgcttata cgattacgct actgattgct gggatttttc acttatctta taaatatcag
    76321 ctgaataaaa accacttgaa tcggactttc cgctaaaaaa tatccgtacg ataagtagag
    76381 tgccttgaag atcggcaaaa aactgccgga ctttttagag gctgtgacaa agattttgtc
    76441 acagcctctt tttccatata aacggcgttc gcctaccagc tgcctggtaa atctgtttcc
    76501 tcttcgttaa gaagcgaaaa tccgtcaaat gtttcgccat cataataacg aagcagctca
    76561 tccatggaca agttcatcgt tttttctttt tcttcttccg ctgattcagc cagtagctgt
    76621 tcccgataca cctgatacat accagctatt ttttgctgca tcgcttttct agtttcacct
    76681 tccgcattga ttattggcaa ttctttgata tagccacgat aaatcaaccg atcatctgaa
    76741 aattcaatgg gtaattcaac gagtgtcaac ccgacttcct gaaaatattc ttccagcatc
    76801 tattatcccc ccattctttt tcttaaatga attttagctt acctactgaa aattttccag
    76861 tttcattatt ttgagaaaaa aagggaatgt gacaaaaatc gtgtcacatc ccctttttcc
    76921 gaataaatgg tgttcttctg ccaactcctc aatcataagt cacaatattc aacaaacatg
    76981 agaaactgtt ttttgaaatt gttccttgtg attcagagtt aaacgacagt ctcacaacct
    77041 cttggtaaac ggcgttcaat cagctgaccc tcggtattag ctcaaaaaac ggtaaaatat
    77101 gagaagctat ttttccgttt tgtttctcta atacttagga taaaacgctg attttacaac
    77161 ctctgttaaa cggtgttcaa tcagctgtcc ttcggtatta gctcaaaaaa cggcaaaata
    77221 tgaggaacta tttttccgtt tgtttctcta atactcagga caaaacgctg attttacaac
    77281 ctcctgataa acggtgttca aaagcttgca tctgcggtaa taagcccaac caaacgaaaa
    77341 atatgagaag ctatttttgt ttggctgttc ttatgacttg atgcaggaca gctttttcac
    77401 aacctctgat ttcttattct tcttcatttg tagcaaaata attacggtga taaatgatac
    77461 aacatacact gataagaaca atggcacctg caaaataaaa cgcatagctt tcaccaatat
    77521 gttcaccaat cacacctgca ataaatggtg ctaaagctcc accgaaaaag cggacaaaac
    77581 tatagctgga cgaagcaacg tttcgttgca gacctggtat ttccatagca atcgtcgtca
    77641 acagtgtatt gataattcct tggaaaaatc ctgctaacac aatgctgatc ggtacaagga
    77701 ctggcataga agttccagct cctaataaca gcaaacaaat aatgaataag acaaatgctg
    77761 ctaaaattgt ccgataagtc gtaaacttcc gctcaagagc cggtgcaacg aaaatcgaag
    77821 caatagccag aagcacaccc cagccaaaaa atacaaaacc gacttctaat tcagaatagc
    77881 caatcaataa aaatggcgca tacgcaagta aagtaaagaa tccgaaatta tataacaatg
    77941 cgattagtcc aactgaacga agtttatgat tgcttaacgc tttgacacct tcccaaaggc
    78001 ctacttttct ctttggtttc tcagtttcag gtaaaagtac cgctagggct ataaaaccgc
    78061 aaaacatcaa aaatgctacc ccaaaaaatg gataacgcca tgaaccactg cccaagaccc
    78121 caccgactag agggccaaca gccattccgc agccaagtgc tgcttcatac atcatgattg
    78181 ctttttctgt gttaccaact aaaatactga cgatcgttgc taaagcagta gagacaaata
    78241 aagcattccc gacaccccag ccggcacgta acccaatcag cattccgata ttcccagata
    78301 atccgccaag accagcgaat acaacgatga taaacaatcc tgtcatcaat gtttttttca
    78361 aaccgatccg actagataag aaacctgtaa atagcatcac cacaccagtg actaacatgt
    78421 aactagtaaa taacagggtc gtttcagctg gcgtagcgtt taactgtgca gcaatcgttt
    78481 ttaagattgg gtctactaga cccactccca taaaagcgat catacaggta gctgctacaa
    78541 ctagcgctga acttttcccc tttgtttctt catttgtttg ttcttgtata tctatttgat
    78601 tctccaatat ttccatccct ttctctctat tagcatcata catgatttat tttacatgtc
    78661 aatatgcaca tgtaaaacgt ggaaacttta ctgtgatata ataaagaaaa taagacgaga
    78721 ggtaggattt ttatgaatac tttagcctat atattattat gcgtgctttt atccaaacca
    78781 cgttctgggt atgaattgaa gcagctgatc acaatttttt gggaagccca tcacagtcag
    78841 atctatacaa cgcttgccaa gttagaccag cagggttatg tgaccgtttt ggacaaagac
    78901 gagcactctc aaaaaaagat ctaccacttg actgatgaag gacgtgctct tgtagaggaa
    78961 tggatcaaag aggaaactcc cgctcctacg caaaaagatg aatttctagc aaaaatctat
    79021 gctttttcga cgctcgataa aacaacagct actggactgc tattcactag agaacaacag
    79081 ctgaacaaaa tcatgcaaaa aaatcaaaaa aaattgaatg acctctcgct agacaagaaa
    79141 caagaatttg gacgttatgt tgtgatccag cgcagaatta tgctttgtca gcaggaactc
    79201 caatggtgtc agcaagtaag agaacaaatg cagcagtttt ttgagtaagg gataaacaga
    79261 acctttctag aaatatctat tgaagagcaa attatcttca ataaaagagc aatacagttg
    79321 gaaaagaaaa agcgatccga acgacaggcc aaaacctatc gttcggatca ctatttttct
    79381 agaactgcat tcaagagact atgacaaaaa gcatgccaag gccttttttc aaataacagt
    79441 gttccatcat tcattacttt gaattgcttt tagcacactt cttgaaagcg ggccaacgat
    79501 gatcaactgc aacggtaaag cggcaatcac attcattttc caagcattca tataagcaga
    79561 gaataattga tcaggtattc cttgttccat gatcacaccg aataaagaca tacatgtcac
    79621 cattcctaaa accatcaagc atgaaatagt taagatcatt tggaccttgc tttctggatt
    79681 tactggtagt ttaaaggcaa tttttttcgc cagcacaccg atgatgaaga catccaaaat
    79741 gaaagccacc acgaatccag gcactagacc aatcaacaaa cttgataaat gaagatcttg
    79801 atgcagccat aaattatacg cgctcatccc caagaccatc aaaaaacaca tcattgttgt
    79861 aaaaattatt ccttcttttt tatttgtagg caatctgtac cactccttta aatttgtcac
    79921 gaaaatcatc gtagcacatt taaaaatttc tcgtaagtac tttttttatt ttctgccctt
    79981 ttatttcata aacttcatca gccgtacgct cgacaaagta agaatcatga gaagtaaaca
    80041 aaatcgttcc cggatatatt tgcattagct tctctaaagc ttcgatcgtt gccatatcaa
    80101 taaaattagt cggttcatct aacagcaaca cattacttgg cttagtaaaa agcaaagcaa
    80161 tcgtcagacg ggtcgcttct cccccactta atgtacataa cggtttcgtc aaagtttcct
    80221 gagcaaaccc taagtgattc agcaaactac ggacaagcga ttctgaaaac tctgtttgtc
    80281 tcatgacaaa acgaatgatg gattcttcag atgtcatttg ataacccttt tgctgatata
    80341 cttgaaagct tactttcgga gagagaagga ttccttctcc ttgtttgcgg atatgttcta
    80401 ataaggttgt cttacctgaa ccattttcac cgacaatcgc gattttttta cctaaagaaa
    80461 actgaaaatc accatttacc agtaatgttc tcccacttct ttccaattgg acattttgtc
    80521 ccatgatggg atagcggctg tggagttcaa gagattttgg tactggaaaa cggatttgtt
    80581 tcatctgctg cggcttggtt gtttctccta ttttttggag tcttctctct aatgttttcg
    80641 cattcttttg gatggctttt tgtacactgt ctttttgttt agaggaagac aaccgatcca
    80701 gtctgatttg ttgctttttc tttttactgg acactttttc caacttttcc gcttgttttt
    80761 tcttttcttg gatcgatttt tccaatcgtt ttttctcttt catgatcgac tctgcttctc
    80821 gtagctgagt ctttttctcc aattcctttt gagtgagata ggcactataa ttccccgaaa
    80881 actctcggat ttctccgtct tttacttccc agattttcga tgctagctca tctagaaaat
    80941 agcgatcatg actaacaaaa accagcgtac cataataata acgaagctct tcaatcagtc
    81001 gatcaatact ttttctatct agatgggttg ttggctcgtc tagaagcatc ccttgttcat
    81061 aagaagaaag aagacgtatc aactcgatct ttcgttcttc ccctccgctc catttccgct
    81121 ctgaagtttc ttccagtctt aactggaaat gactggctaa ttccaaattt aggtcctttg
    81181 cttcagcgag ataggttaat tgaggcaggt agttgaaggt gatctctgtt tggattttcc
    81241 catgatcggg aaaaagttca ccggctatta gtttgagcaa agtcgattta ccgacaccat
    81301 tttctcctat gatccctata cgatcttgct gatagacacg aagagaatca atagatagtt
    81361 ctagctggtt gccaaagctg acttgtagat ttgttatatt tactgctaaa ttttccatat
    81421 gttttacact cctatttcat gtaaaacaac aattatcgat actaaaaaag agcagaaaaa
    81481 tctgtttgca aaaaaactgc atcacatctt ttcctactct gaacgaaata actatgacta
    81541 aatcaccaaa tctgtcatag ctttcaccta ttctactgtc gttcgaaaaa atggacatac
    81601 tccatccatg aaattgttgt ttatacatgt cttttgaatt tatttagttg ttattcaaaa
    81661 gttcgaaacg taatttcatc gatgcagtca tttttccgac ctcctttttt attaggttac
    81721 cacacacgat tcaaaaaaag caatagctac tgaatagtaa ctgaaaacct tttattcgaa
    81781 catagtgatc ttttctggtg ctaaacgaac agactcgtaa ccttcttcta ccgcttttcc
    81841 tattgataaa ataataactg gaacataacg gtctttatcc aaaccaaatg cctctgccaa
    81901 ctgatctgct tcgaatccac caattggatt tgtttcatag ccatgatcac gcgcaactaa
    81961 catgaattgc atagccgcta agctagaatc gactttgact acatcgttca tttcttctct
    82021 tgaaaaactc ttgtaataag gaatgatcgc agctaattgt tgatctctga cttcttttgg
    82081 catcttccct gattcgtaag cttggttgta gatttcttcg ccatattcgt aacattgcat
    82141 atcgccaaaa atcatgacca ttgcagaaga agtatcattt tgtctcgtat tgaaacggat
    82201 caaaggacgc aagatttctt ttgcttcctc gctttcagcg accacaaagc gccatggttt
    82261 catattgaca gaagaaggtg cagtcgtcgc ttctttgatc atttccagca tttcttcgtg
    82321 tgggattttg tatgtttcat catatacgcg aacggatctt ctaccagtca taattgttga
    82381 aaaatcattt ttttgttcca ttattattaa cctctttcgt ttctgttgtt atattgcaaa
    82441 gttgggtatt caatttatct aaaagtgtta gaaattgttt catttctaca tcgctgaaag
    82501 ccggggatag cacggaacct gtatctttat ggtccatttc acaatgcttg attttcttct
    82561 gggctttttc tgttagcgct acatacactt cccggttgtt ctgaggattt ctctgacgcg
    82621 aaacatagcc atttgcttcc aataccttca gatgacgagt aactgctcct tgatcaatct
    82681 gcaacttttc ttgcaaactg ctttgtagca atggattttc ttctttcagt ctcatcatga
    82741 tctcgtatct agtaagactt ataccgatat tttcttcaaa gatctggacg attttttgat
    82801 cggctaattt gatttgatat aaaaggtgac taatctcttc taaagacata atttcctcct
    82861 ttctaataat tgactcgtca acgattgact tgtcaataat agaataaaaa aagaaaaaag
    82921 tcaaagaatc agctgtgtaa taaaaaaaac gctctcaata gaaaagtata gagaaaagaa
    82981 attttttgta gaatgaacgg aaaaaatgat aaagaaagca caacgctaaa accaagatta
    83041 ttgattctta tcaaactata gaagatagct cttctgcagt agataatttt acaccgccaa
    83101 aaatttccga aatcgccttt tgcaagaatc ccgtcgaata ttagaaattg gatcagtatg
    83161 cttaaacagt aataaaaaag ttatgacaag atgttgtcac aacctttttt gagtaaacgg
    83221 tgttcaaaaa cttgcatctg cggtaataag ttcaaccaaa caaaaaacat gaaaatctgt
    83281 ttttgtttgg ctgttcttac tacttgatgc agggcagctt tttcactacc tctttttaat
    83341 aaccataaaa tcctattcct tcatatctcc agcccatcgg tgttagccaa tctctttcct
    83401 tagcactcaa ggtatagtga tgggtaccag ttttttcatt tggattaaat agtcgataaa
    83461 ttggcttgga accaccagaa taaaaactta gaccttcata acgccaaccg atttttgtta
    83521 aagagttgat ctcagattgt gccattgtgt aatgatggtc tccactgtaa ggattgtata
    83581 aacggtagac aggttgtccg ctgtttggtg cttgccaagc aatcccttca tattcccagc
    83641 ctacagttgg cagataattt ttttctccgg cattcaatgt atataaatgt tctcctgtat
    83701 gacggttata cagacgatac atcggagaag ttgaagttgg tgctgtatca tactgtgtca
    83761 gattataggt ttcgattaca ttgttcaatg tagaaccata attaggcgca gtggcataac
    83821 gacctgttag ccaagccgta gcatctttgt aagagttcgt attgcttttc catgcaccgg
    83881 aatagtagta aacgcctggc tgaaaagaag tcgtcttcaa taccgttacg ttatcttgca
    83941 atgattcttt gtacgaagga tattttctaa aagcggcatt caccgtaatc cattgcccat
    84001 tcacatattc cgaagtaggc atcgtaacag attggccatt atagcttcct ttaatcccaa
    84061 atagattgta attaggggca gaagccaaag tactgttccc ccagccactt tcaacgatgg
    84121 cttgagcaat catgactgat gcatataagt cattggcact tgccaaactt tgtgcagttg
    84181 aggcaatact atcaatgaac gcttgttgag aattagcaga tgagcgagca taaaaagcat
    84241 agccagtata gttttcgaat tcagacaaat ctgcttgatg ggtatgtgtc aatgtttgcc
    84301 catctgtcaa ttggttcgtg atttcttctg cattctctgg attgttctga gcttctgctg
    84361 atgtactgtc ttcggtttgt gtttgttctg ttgtttcagt tgttggttct tcggttgaaa
    84421 ttgtattttc tgagacagta gctgactgca aagtactttc tgtatcgttt tcaattcctg
    84481 tttctgaaga actcgatgtc tgagttgcga cagtttctgt cgattcttca gctagcactg
    84541 ttgtgttggc aactgctaga tttcctaaaa gaataacgct aaataataaa ttctttgctt
    84601 tcatcttttt tctcctttga attatgaaac ttttcctaaa taacagtgta actaaaaaag
    84661 taaaagcaag tcaatgtcca tatttaaata acatttgttt ctttttaata aatttgcttt
    84721 ttctgcataa aaagacgtaa atgaagaata ttcttctcca ttcacgcctt aaattgatgg
    84781 tcgtttgaat aactatttaa caagtaattc tctctggata ttatccgtta tggattatac
    84841 ttcctgtgtc gtcggacgga taatcatgtt gctgacagac atccggtctg gcgtttctat
    84901 cgcaaatgct acagcagaag ctatatcttc agatgttaat ccccattctt tttgcgcttc
    84961 atgaagttca agagcagcct ttttgtcgct gatggtttga tataattcag tttgaacagc
    85021 tcctggagag atgatcgtag atttgatgtt attttgccgt tgctcttgac gtaatccttc
    85081 catgattgca cggacagcaa attttgttcc gcaataaacg gcggaatcag gataaacaac
    85141 atgtcccgcc acagaatcag ttgaaatgat atgcccagat ttttgttttt ccatgattgg
    85201 aagtacggcg gaaattccat ttaacacacc catgatgttg atatcaagca tattttgcca
    85261 ttcatcacgg tgaccttcaa ctaatggagc ggttggcatg attcctgcat tattgtacaa
    85321 tacatcgatt cgaccgaact tttccatcgc taaatcgata actgcttgga cttgtgcaaa
    85381 atctcttaca tctgctgttt gaatatatag ctcagcttct ggtaaagatt cttttattgc
    85441 ttttaggcga tcttctcggc gtgcagctat aactagttta gccccttttt ctgctaatag
    85501 cttcgttgtt gcttcaccga ttccgcttga agcgcccatg atgacaatca ctttttctga
    85561 taaatcattc attctattct cctcctgttt tgaaaaatct cttgcttata ctcactatct
    85621 cttatttcat cttactgttc aaataatccg ttcgacaggt ttgacgattt tggcagggat
    85681 tcctgcaacg atggtattgt ctgggacatc tttcgtaaca gttgcattcg ctgcaacaat
    85741 ggcattctcg ccaatcgtta cgcctggcag aatagttgct cctgctccga tccaagcatt
    85801 tttcttgatg tggattggct ttactgacaa gcctcttcgt tcttttggat cggttagatg
    85861 gttcaccgtg atgaggttga cacgaggacc aatcaatact gaatcttcta aacagatgcc
    85921 acctaagtca acgaacatca cttgacgatt gataaagaca tttttcccta tactaatatg
    85981 tttgccgaaa tctgtataaa aaggcagcag aacagtcacc gtatcatcta ctttgtcact
    86041 aatgatattt tctagccgtt tgcggatatc tgctggttca tggtacccag tattcagcgc
    86101 tgctaaaggc tcttcgtttt ccgcttggat ctgatggatc acttcaaaga gcggatccgt
    86161 tactcgaatc tctttattga cgatttgttt ttcgatgagg cttgtcattt tgctccctcc
    86221 ttttatcctt actttataac ggacggctta tgatgtctaa tacttataaa acatacttaa
    86281 ctattccttt taactatact tagtggtatg cttttttaaa aacaggaggg aaaaaatgga
    86341 tattcgtata ctgacgtatt ttttagcagt agctagagaa aaaaatatca gccgagcagc
    86401 aaaatctctg catatcacgc aacctactct ctcaaaacaa ctgaaagatt tagaagaaga
    86461 acttggtgtg acacttttca ctcgaggctc ccgagagata cagttgacgg attctggtcg
    86521 atatctttat tcaaaaggaa aagaaatctt gtcgttggta gaaaaaacgg cgaacaattt
    86581 aacaaatgac gcaatcatct ctggagaaat ctacattggc ggaggcgaaa ctaaagcaat
    86641 ctctttcatt tctgatagcc ttcattcgtt gatcaaggcg catcctgata tccagtttca
    86701 tttatatagc gggaatgcag atgatatcat agacaaactg gatagcggat tacttgattt
    86761 tggactcgta atcgaaccga caaacaaaca aaaataccag tcatttcgac tgccagccca
    86821 agatcgttgg ggacttttga tacatcggaa tcatccttta gcaaagcaaa cagtcatcca
    86881 tccgcaggaa ctgaaaactg ttcctttgat catttcccgg caaacgtcag tggacagtca
    86941 actagctgaa tggctaggtt tcagcataga tcagttatcg atcgtcggaa catataactt
    87001 attgtacaat gcttctttga tggtcgataa cgggactttt ggtgcattag ctctagatgg
    87061 gatcatcaat acttatggga cggaaaaagt attcatccca ctcgatccgc cattacaagc
    87121 ttcactgaat ctcatttgga aaaaagacca gcctctttct cctgctgctc gtaaattttt
    87181 agatgtccta acggagaaat acagttaagt agagtttttt tccttcgctt tcagcttttt
    87241 aacaacgaat aagttaagca aaagatccaa tgattaaata acataagttt cttaatttgt
    87301 tccttccttt tatttctgat aattccagta aattactata attataaaaa aagaattgac
    87361 aaaacataac tgtctatata aaattgatta cgttaacaat aattcgtttc atgtgactag
    87421 atcatactag gcatgaaaaa tctctgagga acatggtttc cctaggaggt ttttcgtgtc
    87481 tatttttgtt tggccgcaat caactggtct agcaaacgtg aaaaccaaat cttcaaaaag
    87541 ggtgcgcgca tgaaaatcaa aaagatcttt aatcaaaatg cagtattggt agatgacggt
    87601 ggacaggaaa aagtagctat tggaaaaggg attggatttg acagaaaaag aaatgatttg
    87661 atttttgatc gtgatgtcga acggatattt gtcatggaac cagaaggtca aatcaagttg
    87721 caaaatttac ttaatcagat tgatgaacgc tttttatttg cagcagaaac gatcattgat
    87781 catgcggaaa ctgttttgat ggaaaagttg aatgaacatg tattgatcgc tttgaccgac
    87841 cacattgctt tttctgcgga gaacattaat aacggtatcg tcatccgcaa caaactttta
    87901 agagaaatcg aagtgcttta cagtgatgaa ttcagtattg ctcaatgggc agtagagtat
    87961 ctgacaaaag agcttgggat tccatatact tatgacgaag caggatatat cgcaatccat
    88021 attcacagcg gacgaagcgg tcggaacaat aaccatcgca gtatccgtga agtgacgatt
    88081 gtatctgaca tcatccattt agtggaatca gaattggaaa cagatatcca ttcagaacca
    88141 ttttcactga attattcaag actggtgaat catttacgac tgcttctaca acgaacccat
    88201 gcgcaacaat atgctgtttt agacacggaa atcgtagaaa tggtcaaacg aaaataccca
    88261 gaaagctata agatttctaa aaaaatccgt gtgttgctga ccaaagaata ccagctcgcg
    88321 atcacgaaag aagaactggg ttacttggcc atccatatcg agcgtttaag aagcgcaatc
    88381 gtacaaacaa atgttaacaa tcatgaggag gaaaaaaatt gaaagcatat ctacaacgaa
    88441 tgggacgttc ccttcaactt ccagttgcgg tcttacctgc tgctgcttta ctagtaggga
    88501 tcggaaactg gtgggcatct tatagtaacg acattgtcgc tcatttttta caagctggcg
    88561 gtaacgcagt tttaggtcaa ttacctttgc tgtttgccgt tgggttagca ctagggatgt
    88621 cagtggacaa atcaggtgct gctgccttgg caggtttggt cgcatttgaa gttccagtaa
    88681 atgttttaaa aactgattca gtagctactt tattgaatat caaaccagaa tcagttgatc
    88741 catcttttgc tcagatcagt aacgtattta ttggtattat tgccggattg attgcggcag
    88801 ctctttataa tcgtttccac gaagcaaaat taccgatggc tttatctttc tttagcggca
    88861 agcgtctagt gccaatcttg gctgcctttg tcatgttgct gatcagtgca gtgctactgc
    88921 tcgtatggcc tccggtttac tctgctttag tttcttttgc taaattcatt ttgagtttag
    88981 gctggggtgg tgcaggtctt tatggcttct tcaaccgttt actgatccct acaggattgc
    89041 atcaagcaat gaactccgtg ttctggtttg acgtagcagg aatcaatgat attggtaaat
    89101 ttttggcttc tgaaggaaca aaaggcgtaa caggaatgta tcaagctgga tttttcccaa
    89161 tcatgatgtt cgggcttcca gcaggagcat atgctattta cagaaatgca cgtccagaaa
    89221 acaaagcaaa aacagcttcc ttgatgttag cagcagcttt tgcttcattc tttactggtg
    89281 taacagaacc acttgaattc tcatttatgt tcgttgcttg gccattatat gtcttgcatg
    89341 caattttcac tgggatttct ttagcagtga gtgcattctt ccattggaca gcaggatttg
    89401 cctttagtgc cggatttgtc gattacttcc tatctttgaa aaatccgatt gcgaatcagc
    89461 cattgatgtt gatcgtccaa ggattagtaa cagcagtgat ttactacttt ggctttaact
    89521 ttgctatcaa aaaattccat ctgatgactc ctggccgcga ggaagcagat ctttcagatg
    89581 acgatactgc aacgactaat tcagacaata aatatgcggc acaagcaagc aaaatctacg
    89641 ctgcattagg cggagccgac aatgtaactt cgatcgataa ctgtactaca cgccttcgtt
    89701 tgcaagtcaa agatacaggc ctgatcgatc aaaataaaat caaagccact ggcgtacctg
    89761 gtatgaagat catcgacgga aaaaatgcgc aagtcatcgt aggtacagaa gtacaattcg
    89821 ttgcagatga gatggcaaaa ctacatggtg gtgctgcaac acgacctgca caaacaaaca
    89881 ccgtggttaa aacagaaaca tttgccggtg gaacgatcga acaagatatt tacgctatcg
    89941 caaatgggaa actgattcca atcactgaag tttcagatga tgtattcgcg gaaaaaatga
    90001 tgggtgacgg ctatgctgta ttaccagaaa atggagaaat cttctctcca atcgctggta
    90061 cgatcacaaa cattttccca acgaaacatg ctgtcggaat ccaaacagat gctggtatcg
    90121 aagtcttgct tcatatgggc atcaacactg ttgacttaaa aggcgaacca ttcactttat
    90181 atgtaaaaga aggtcaaaaa gtcgcaagag gtcaattgat tgctttagtt gatcttgccg
    90241 ctattcaatc tgcaggcaaa aacacggata tggtcgttgt attcacaaat ggcgacaaag
    90301 tccaaagctt agaaatcgaa cccgcacgtg atgtaaaagc aaatgataaa atcggctctg
    90361 tttccaacaa agcctgagcc atcgtcttcc ccagactttt ttatcgataa acaaacagga
    90421 gctgagacaa aaatcttgtc tcagctcctt tttccgaata aaaacggcgt tctcctgcca
    90481 actccgcggt aataagtcca gccaaacgta acatatgaag agctattttt tatttgactc
    90541 ttcttattac ttgatgcaga acagcttttt tacaacctct gataaacggt gttcaaaaac
    90601 cagtttatcg gtatgaagtc aaatttatgc aaaaatgtgg aaagccattt tcacaaaatt
    90661 tttcttcata ctcaaaacta atcggctttt tcacaacctc tttatacatt attcctgatc
    90721 caatatttct tttaatgctg cttcaagaga agttaatggt tttcccaata ctttttctaa
    90781 atcatcagag gaaatagcta actgatcctt tttaatatca tgttggatcg ctaaaaacat
    90841 gtcagctgct tcttgaggga atccggcttc ttttagattt tcgacaaacg ctgcatcact
    90901 tgcttcaata atttctaact ttttacctgt cgcacgctct aatgccttgg ctaattcttc
    90961 atagtcagtt agattgccgg ataattctaa gatttctgga gagtcacttt ctactagtac
    91021 ttttgctgca gcttctgcat attcacgttt caatgcccaa cctgtttttc catttcctgc
    91081 agcataaacg aattgaccag tttttaaagc ctgcccaatg ataggtaatt cattctctac
    91141 ataccaatta ttccgtaaaa atgtatggga aatacctgaa tccttaatca ttttttcagt
    91201 gaattggtga tctggcgcta atacactgat cgagtgatcg gcatctgcaa aacttgtata
    91261 agcgatataa gataccccag ctgcttgtgc tgccttcact acattcttat gttcggcttg
    91321 acgatttcct ggaacgccag agacaaaaag caatcgatct attcctttca acgcttcttt
    91381 cattgagtct aaatcatcat aatcaccaat acgtatatta aatccagctt ctttcagtgc
    91441 ttctcctttt tcttcactac gtactagtac atagatttca ttttgtggaa ccatcttttt
    91501 taagaattcc agcgcataac caccaaaacc tccagttgct cctgttacta aataatgcat
    91561 atttgccact ccctattctt tttatttgtt aagatttaca gctgtacatt tataaattac
    91621 acctttatca taaagagtgt ggtaagataa gtcaagcaaa aaaaatgaaa ggatgacaac
    91681 tgatgaagta ttctctgcaa tttagtgatg caatccatgt acttgcctat attgaaatgt
    91741 ttcaagatac ccgatcactt tctagtgaga tgattgccag tagtatcgaa acaaatgcag
    91801 caaatgtacg taaaatcatg agtcagctca aaaaatccgg tttgatcttt acaaaaaccg
    91861 gaaaaccgca gccttccctt gccaaaagtc cagatcagat ttctttacta gatgtctatc
    91921 gcagcatcga aggtaataca cagcttatcc aagtcgatcc aaaaacaaat ccaaattgcg
    91981 tcgttggtgc gaatatccaa gaagctttag aagaaagcta cgctcggctg cagaaaaagg
    92041 cagaagaaga aatggctaag atcacacttg atagtctgat acataaaata gccgttttgg
    92101 aaaaagaaaa aagaccagaa aacacaggcc tcgtagagag atttttataa ttctctctac
    92161 tgtgcctgca tttttctgat cggaatcgtc tacataaatt cttttattct ccgggggaca
    92221 aaagccttgt catgcccctt tttcctctct aatgctcagg tcaaagcgct gaagtcatga
    92281 cgtcttgata aacggtgttc aagtagctga ccctcggcat tagttctgaa aaatgcaaaa
    92341 tatggaaagc tatttcccat ttttcctctc taatgctcag gtcaaagcgc tactttcacg
    92401 acctctatat tatcatggtt tattgactac cggtatccag atttctccca tatatttccc
    92461 gttggtaact cccatttcga ctacagcatt tggaccccca acataggcgt aatcttctac
    92521 ttctcttaaa acatgcccaa aggccgtatt cgtcaaatca tttcttaatt cttcaggcga
    92581 ttctgctaaa gcgattacaa caagatacgt tccttttggg aatgggatga ttcgggtagt
    92641 gtttggaaca tcctgactcg tttgaacacc gacatagtac atcattttcc cgttataggg
    92701 ttcgttgact gtaaagaaat aatgattttc agcagcttgt ttcaatgcat ctattgttcc
    92761 attaccgata actttttgct caaatgcttg tttctctgca gccaaaccga caaaatccgt
    92821 attgctactc ttcaattcca caccaatacc cgtcaccgta aaatctggtt tttcttcgat
    92881 cctatacttt gccatttctc ccaccctttc tattatatta agttcatctt acataaaatt
    92941 aaagggaaat gacacgatta cgctcttttt tctagggata tttttatttt ttaactattt
    93001 ttttgctttc actgcaatcc aaatctcact gtaaacatta ctgaaatcct ctccccattt
    93061 tgtaaaagag atctcgggag cttcgactaa ctcataatct gaagaaggaa gccattctgc
    93121 atagattttc ccccaagtat cttgtaaagt ttggggaaat ggccctttat taggaaaaat
    93181 ggcccaagtg tgttcttcta tagcaagcat ttctaaatca gcgtacggat tttcttttgt
    93241 cgtagcaaag ccaatcatgt gcatcaaact gcccttttct tccaagcggg ctccatcaaa
    93301 tccataagaa acgttgatta cttgatttgg atataaatct cctaacttat gcatctcttc
    93361 cctttgagtc tccgtgatcg tctgcgctaa cttttgtatt tcttgattga ctccttcaaa
    93421 tttgacagga acttttttcg ccacacctgc cacataaaac tgttctttct tttcgatacg
    93481 aaattccatc gattttcctc ctctgacatc aataaaaaat gtcaagcgtg gaaaagattt
    93541 ttgtatgccg tatctgctga tttctgaagg cagatatcct gaccaatcac gaaaagctct
    93601 tgaaaaacct tcgactgttt catatccata tctaaacgcc gtttctgtca ctcttgatcc
    93661 atctaggaga tcgcgattag ccaaagccaa tctacggttt ttgatatagt ccattagcga
    93721 catccctgca ataaacgtaa agatgcgttt taattgatat tcagagattc ctgtattttc
    93781 ggctattatt ttataggaaa gtccttctgt caaatgttct tcgatgtaat cgatcaattg
    93841 gttcagatct ttaagcatat ttatcttcct ccttgcttgc ctttttatca tattatcaaa
    93901 gaaagtcaat tacttgatga atcaggaacc aatttagtca gatgtagaaa atgttgtatg
    93961 gatctaaaga aaattctcaa gcagcgtttc gtaacggata attggtatta tctgcctatc
    94021 tatgttttac ctttagaaaa ataaattttg gggaagacac attctaagta aacaaagcgg
    94081 taaaaatatc ttttattccc ttagttaatc cgcttactac ctactgtaca aagcaaattg
    94141 ttgttcaaac tgctgatcag aacgctgctt ggatttttct ttgcgataca tcatacctct
    94201 tttatcaatt ggaacaatta tttatttcta acaaaatcac ttcatttttg tcagataaat
    94261 aaaaaacaaa aaaagttcca tctttaaaac acgctattct tttacttttt ctcacattct
    94321 ctaagaaaag aaaaggagaa tgaatttttg acaattcgag caaaagtgat tatcaaatat
    94381 gtaaaatcag ctactttttt cttaaacgtt tctttatact aaatataaat aagtgtttca
    94441 tgaaaggaga actagatgaa taattggctt gagtattttc ctgaaaacgt tttggaacgc
    94501 ggttacagct atcatctgca cggttttgtc cgtcatttga actatacaag caagtacttg
    94561 tccgcaacag tttctggcac ggaagattat aaggtagtga tcacttggga tgaaaagacc
    94621 aacatgactt gtgattgtct atatgccata gaaggaaaaa aatgcaaaca catggctgct
    94681 gtcttattcg cttacgaaga acgtccaata aaaaaatcca actactcact ttccgaactc
    94741 tcgtcccttg tttcctccgc tagttcctct cttgtacggg agcttttaac agagattctt
    94801 atagaacatc cgcaatttat cgaacgcttc aaagttaaga tgccatttca tgcaataaat
    94861 tattcagata aattaacaac tatcatccat aaatacgatc atattatcaa gaaaaacaaa
    94921 aacaggaaaa cagcgaagtt catcatggag atgcgaaagt tcatccaaga agcagttgaa
    94981 tcgctcattc agcaaaatgc ctatcttccc gcattcgaat tgatcaatga agtgattgct
    95041 actctcgaaa cattctattg ggagcctgag gatgaacgaa cccttcttct gatagaggac
    95101 tgttattatt tatggaaaga attattagca gaagcacctc atgcagaaaa aaggcaaatg
    95161 ttctcttggt tcgtctgcca agtcgatcat actgacgcct cttattcaaa acgctactcc
    95221 ataaaaatcc tgaaagaaga ctttcgggaa aaagaatttt ctaaccaaaa gaaaaaaata
    95281 gataaaaaaa ctaaagaaac ggtaaagaaa gacgatttca atgaaaaata gcaagtaaac
    95341 gataacaacc atcctaaatc cacaagaaat acagcagacc ttgctttaaa aacacgaatc
    95401 atgcaaagga tgaagtaaag aagacttaac gcgtacccaa tattagtcat ttgactatta
    95461 ggtacgcgtt ttgttttata agtcatgaca aatttccttc gttgctgggt agattttccg
    95521 gcattgttga tagattttat gatacttttc tacattttgt aagagaggtt ccactgcatc
    95581 tgtatattgg ataaaattct gacaacaact ttcaggtgtg tcgaaccact ctaatcttta
    95641 aagataaata ttaaaataac gattaataat attaacagtc tttatttttc accaccaaaa
    95701 acctatcttt gcatatacta aatagacgta tatacacaga taggtttgtc ttgtgaaaaa
    95761 taatgattat ccttaccaaa aaatgtttgt ttttctacat gttcgtactt atcaaaaaat
    95821 atgatcctta ttgaaattac tggctattta tattccaacg acttcttgtg ttaatttttg
    95881 ttatctcata gtaaattatg tttcattttc acttaattta gttgtcacgt gttcgtcaat
    95941 tcgtaaaaac ggtaaatata taaacacact tgcggctata attaatattt gtaaaacagc
    96001 tgctcgccag tctccggctg ttgctaaaaa tcccgatata actggtggcg tagtccaagg
    96061 aatttgcact actacatgat taattaatcc cacagcagtt gcaaaatatg ctactgtcaa
    96121 agaaaaaatc ggtgcaagca caaagggaat aatcagactg atattaaata caataggcaa
    96181 tccaaaaata ataggttcgc ttatattgaa tatagcggga gttaccgata atttagcaaa
    96241 gtccttataa tcttttctac gactgaagat aaatattgca atcaataaag caatcgtatt
    96301 tcccgatcca cccattaccg caaaggtatc tttaaaggcc atattgataa tatgtggaat
    96361 ttgctctttg tttgcataag ctgccatatt ttcttgcata ttctgaatca aaaacggatc
    96421 taacaatgaa cctgatatta ctgcttgatg gataccaaat ccaaaaaata gattagcaat
    96481 agatgtaatt aacaaaaatc ctggtaaacc agttgtaatt ttacttaatg gctctgtaac
    96541 tatcgtggta attagcttat ttatatccat attaaacaat tgaatgatta taaatgacaa
    96601 taaagcaaac gaggtaatcg taataatgat tgggattaat acattaaaag actttaaaac
    96661 agcgggagga atatttccgg atagctgaat ctgtagtttc ttattattag aaagtttaat
    96721 aaataattct gtagccgcta atccaacaaa tatacctaca aacattcctg aagctcctgt
    96781 ataggatata ggtatcacac ccgtcacttc aacagcttta tccgcatttt caggagtaaa
    96841 catcatctgc ataggtgtta atacaacaaa tgtcgaaata gcaactaata ccgctgcaat
    96901 atgatttgta taatttctgc ttgcacacat atgataagat actgctgcta caactaataa
    96961 tgtagttatg cttaatgtac cattggcaat tgaagtacca atttgttgta acgtggtcaa
    97021 tgtactagta tcgacaattt cccccatgaa tcctgttggt tctaagatca ctgaattaat
    97081 aagtgtcaca aatcctgcta acatcataaa tggcataaat gtagcaaacg catctcgcaa
    97141 agttcttaaa tggatctgat tccccaactt agtcgcaact ataccaaatt tttcaaatac
    97201 aatgtccttc ctgctacttt tgcccatatt ttttctcctt tatgcttatt gtaatcgatt
    97261 actttttcca cccaaataat acatcactta tcaa
//
LOCUS       DACDPS010000003        91381 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.003, whole genome shotgun sequence.
ACCESSION   DACDPS010000003 DACDPS010000000
VERSION     DACDPS010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 91381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 91381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..91381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.003"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1271
                     /locus_tag="IWJ07_000207"
     CDS             <1..1271
                     /locus_tag="IWJ07_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9331817.1"
                     /translation="SLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLILMGCYEIY
                     IRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLKATV
                     LTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYTIGLFLFL
                     GLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVWRLNGFSS
                     LFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLTLIAPEDA
                     VAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIELDNSNNKN
                     TVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTKLAIASRY
                     LSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(1416..2156)
                     /gene="truA"
                     /locus_tag="IWJ07_000208"
     CDS             complement(1416..2156)
                     /gene="truA"
                     /locus_tag="IWJ07_000208"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP9331818.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(2347..2982)
                     /locus_tag="IWJ07_000209"
     CDS             complement(2347..2982)
                     /locus_tag="IWJ07_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP9331819.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(3129..4370)
                     /gene="sstT"
                     /locus_tag="IWJ07_000210"
     CDS             complement(3129..4370)
                     /gene="sstT"
                     /locus_tag="IWJ07_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAP9331820.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            4568..5566
                     /locus_tag="IWJ07_000211"
     CDS             4568..5566
                     /locus_tag="IWJ07_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9331821.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            5579..7597
                     /locus_tag="IWJ07_000212"
     CDS             5579..7597
                     /locus_tag="IWJ07_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAP9331822.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(7657..8373)
                     /locus_tag="IWJ07_000213"
     CDS             complement(7657..8373)
                     /locus_tag="IWJ07_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP9331823.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(8600..9142)
                     /locus_tag="IWJ07_000214"
     CDS             complement(8600..9142)
                     /locus_tag="IWJ07_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP9331824.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(9147..9374)
                     /locus_tag="IWJ07_000215"
     CDS             complement(9147..9374)
                     /locus_tag="IWJ07_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopZ"
                     /protein_id="HAP9331825.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(9453..11333)
                     /locus_tag="IWJ07_000216"
     CDS             complement(9453..11333)
                     /locus_tag="IWJ07_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP9331826.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(11522..12136)
                     /locus_tag="IWJ07_000217"
     CDS             complement(11522..12136)
                     /locus_tag="IWJ07_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9331827.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            12278..12838
                     /locus_tag="IWJ07_000218"
     CDS             12278..12838
                     /locus_tag="IWJ07_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9331828.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(13143..13457)
                     /locus_tag="IWJ07_000219"
                     /pseudo
     CDS             complement(13143..13457)
                     /locus_tag="IWJ07_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(13711..15346)
                     /locus_tag="IWJ07_000220"
                     /pseudo
     CDS             complement(13711..15346)
                     /locus_tag="IWJ07_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15669..16148)
                     /gene="rlmH"
                     /locus_tag="IWJ07_000221"
     CDS             complement(15669..16148)
                     /gene="rlmH"
                     /locus_tag="IWJ07_000221"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAP9331829.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            16576..17865
                     /locus_tag="IWJ07_000222"
     CDS             16576..17865
                     /locus_tag="IWJ07_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP9331830.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(17904..18509)
                     /locus_tag="IWJ07_000223"
     CDS             complement(17904..18509)
                     /locus_tag="IWJ07_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAP9331831.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(18714..19178)
                     /locus_tag="IWJ07_000224"
     CDS             complement(18714..19178)
                     /locus_tag="IWJ07_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9331832.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(19200..19520)
                     /locus_tag="IWJ07_000225"
     CDS             complement(19200..19520)
                     /locus_tag="IWJ07_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP9331833.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(19664..20743)
                     /gene="pepA"
                     /locus_tag="IWJ07_000226"
     CDS             complement(19664..20743)
                     /gene="pepA"
                     /locus_tag="IWJ07_000226"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAP9331834.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            20943..21293
                     /locus_tag="IWJ07_000227"
     CDS             20943..21293
                     /locus_tag="IWJ07_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP9331835.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            21516..22463
                     /locus_tag="IWJ07_000228"
     CDS             21516..22463
                     /locus_tag="IWJ07_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9331836.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(22520..23938)
                     /gene="pepV"
                     /locus_tag="IWJ07_000229"
     CDS             complement(22520..23938)
                     /gene="pepV"
                     /locus_tag="IWJ07_000229"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAP9331837.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            24087..24932
                     /locus_tag="IWJ07_000230"
     CDS             24087..24932
                     /locus_tag="IWJ07_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAP9331838.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            24936..25376
                     /locus_tag="IWJ07_000231"
     CDS             24936..25376
                     /locus_tag="IWJ07_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAP9331839.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            25426..25620
                     /locus_tag="IWJ07_000232"
     CDS             25426..25620
                     /locus_tag="IWJ07_000232"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9331840.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            25756..26442
                     /locus_tag="IWJ07_000233"
     CDS             25756..26442
                     /locus_tag="IWJ07_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9331841.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIRRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(26850..27539)
                     /locus_tag="IWJ07_000234"
     CDS             complement(26850..27539)
                     /locus_tag="IWJ07_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9331842.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(27514..29643)
                     /locus_tag="IWJ07_000235"
     CDS             complement(27514..29643)
                     /locus_tag="IWJ07_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAP9331843.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(29691..31028)
                     /locus_tag="IWJ07_000236"
     CDS             complement(29691..31028)
                     /locus_tag="IWJ07_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAP9331844.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(31280..31639)
                     /gene="rplT"
                     /locus_tag="IWJ07_000237"
     CDS             complement(31280..31639)
                     /gene="rplT"
                     /locus_tag="IWJ07_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAP9331845.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(31692..31892)
                     /gene="rpmI"
                     /locus_tag="IWJ07_000238"
     CDS             complement(31692..31892)
                     /gene="rpmI"
                     /locus_tag="IWJ07_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAP9331846.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(31964..32488)
                     /gene="infC"
                     /locus_tag="IWJ07_000239"
     CDS             complement(31964..32488)
                     /gene="infC"
                     /locus_tag="IWJ07_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAP9331847.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            32808..33440
                     /locus_tag="IWJ07_000240"
     CDS             32808..33440
                     /locus_tag="IWJ07_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP9331848.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(33442..34347)
                     /locus_tag="IWJ07_000241"
     CDS             complement(33442..34347)
                     /locus_tag="IWJ07_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP9331849.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(34432..35085)
                     /locus_tag="IWJ07_000242"
     CDS             complement(34432..35085)
                     /locus_tag="IWJ07_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP9331850.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVAI"
     gene            35407..36351
                     /gene="mvk"
                     /locus_tag="IWJ07_000243"
     CDS             35407..36351
                     /gene="mvk"
                     /locus_tag="IWJ07_000243"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAP9331851.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            36344..37321
                     /gene="mvaD"
                     /locus_tag="IWJ07_000244"
     CDS             36344..37321
                     /gene="mvaD"
                     /locus_tag="IWJ07_000244"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAP9331852.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            37334..38419
                     /locus_tag="IWJ07_000245"
     CDS             37334..38419
                     /locus_tag="IWJ07_000245"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAP9331853.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            38416..39480
                     /locus_tag="IWJ07_000246"
     CDS             38416..39480
                     /locus_tag="IWJ07_000246"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAP9331854.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(39729..40532)
                     /locus_tag="IWJ07_000247"
     CDS             complement(39729..40532)
                     /locus_tag="IWJ07_000247"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAP9331855.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(40552..41394)
                     /gene="kduI"
                     /locus_tag="IWJ07_000248"
     CDS             complement(40552..41394)
                     /gene="kduI"
                     /locus_tag="IWJ07_000248"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAP9331856.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(41410..42417)
                     /locus_tag="IWJ07_000249"
     CDS             complement(41410..42417)
                     /locus_tag="IWJ07_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP9331857.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(42424..43068)
                     /locus_tag="IWJ07_000250"
     CDS             complement(42424..43068)
                     /locus_tag="IWJ07_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP9331858.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(43168..43926)
                     /locus_tag="IWJ07_000251"
     CDS             complement(43168..43926)
                     /locus_tag="IWJ07_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAP9331859.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(44053..45204)
                     /gene="fucO"
                     /locus_tag="IWJ07_000252"
     CDS             complement(44053..45204)
                     /gene="fucO"
                     /locus_tag="IWJ07_000252"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAP9331860.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(45286..45597)
                     /gene="rhaM"
                     /locus_tag="IWJ07_000253"
     CDS             complement(45286..45597)
                     /gene="rhaM"
                     /locus_tag="IWJ07_000253"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAP9331861.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(45599..46444)
                     /gene="rhaD"
                     /locus_tag="IWJ07_000254"
     CDS             complement(45599..46444)
                     /gene="rhaD"
                     /locus_tag="IWJ07_000254"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAP9331862.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(46458..47714)
                     /gene="rhaA"
                     /locus_tag="IWJ07_000255"
     CDS             complement(46458..47714)
                     /gene="rhaA"
                     /locus_tag="IWJ07_000255"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAP9331863.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLEEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(47711..49171)
                     /gene="rhaB"
                     /locus_tag="IWJ07_000256"
     CDS             complement(47711..49171)
                     /gene="rhaB"
                     /locus_tag="IWJ07_000256"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAP9331864.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            49324..50193
                     /locus_tag="IWJ07_000257"
     CDS             49324..50193
                     /locus_tag="IWJ07_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9331865.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(50319..51218)
                     /locus_tag="IWJ07_000258"
     CDS             complement(50319..51218)
                     /locus_tag="IWJ07_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9331866.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(51383..52207)
                     /locus_tag="IWJ07_000259"
     CDS             complement(51383..52207)
                     /locus_tag="IWJ07_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP9331867.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(52218..53513)
                     /locus_tag="IWJ07_000260"
     CDS             complement(52218..53513)
                     /locus_tag="IWJ07_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HAP9331868.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(53529..54008)
                     /locus_tag="IWJ07_000261"
     CDS             complement(53529..54008)
                     /locus_tag="IWJ07_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAP9331869.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(54014..55042)
                     /locus_tag="IWJ07_000262"
     CDS             complement(54014..55042)
                     /locus_tag="IWJ07_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAP9331870.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(55059..56165)
                     /locus_tag="IWJ07_000263"
     CDS             complement(55059..56165)
                     /locus_tag="IWJ07_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP9331871.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(56394..58991)
                     /gene="adhE"
                     /locus_tag="IWJ07_000264"
                     /gene_synonym="adhC"
     CDS             complement(56394..58991)
                     /gene="adhE"
                     /locus_tag="IWJ07_000264"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAP9331872.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(59296..59676)
                     /gene="yajC"
                     /locus_tag="IWJ07_000265"
     CDS             complement(59296..59676)
                     /gene="yajC"
                     /locus_tag="IWJ07_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAP9331873.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(59773..60918)
                     /gene="tgt"
                     /locus_tag="IWJ07_000266"
     CDS             complement(59773..60918)
                     /gene="tgt"
                     /locus_tag="IWJ07_000266"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAP9331874.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            61136..61834
                     /locus_tag="IWJ07_000267"
     CDS             61136..61834
                     /locus_tag="IWJ07_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP9331875.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(62002..64641)
                     /locus_tag="IWJ07_000268"
     CDS             complement(62002..64641)
                     /locus_tag="IWJ07_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9331876.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(65260..66291)
                     /gene="queA"
                     /locus_tag="IWJ07_000269"
     CDS             complement(65260..66291)
                     /gene="queA"
                     /locus_tag="IWJ07_000269"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAP9331877.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            66484..67659
                     /locus_tag="IWJ07_000270"
     CDS             66484..67659
                     /locus_tag="IWJ07_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9331878.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            67663..68304
                     /locus_tag="IWJ07_000271"
     CDS             67663..68304
                     /locus_tag="IWJ07_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9331879.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            68301..69218
                     /locus_tag="IWJ07_000272"
     CDS             68301..69218
                     /locus_tag="IWJ07_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9331880.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            69215..69886
                     /locus_tag="IWJ07_000273"
     CDS             69215..69886
                     /locus_tag="IWJ07_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9331881.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(70427..70915)
                     /locus_tag="IWJ07_000274"
     CDS             complement(70427..70915)
                     /locus_tag="IWJ07_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAP9331882.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(71116..71382)
                     /locus_tag="IWJ07_000275"
     CDS             complement(71116..71382)
                     /locus_tag="IWJ07_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP9331883.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(71382..71645)
                     /locus_tag="IWJ07_000276"
     CDS             complement(71382..71645)
                     /locus_tag="IWJ07_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP9331884.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(71638..71910)
                     /locus_tag="IWJ07_000277"
     CDS             complement(71638..71910)
                     /locus_tag="IWJ07_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9331885.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            72121..72306
                     /locus_tag="IWJ07_000278"
     CDS             72121..72306
                     /locus_tag="IWJ07_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9331886.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(72448..74271)
                     /locus_tag="IWJ07_000279"
     CDS             complement(72448..74271)
                     /locus_tag="IWJ07_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAP9331887.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(74869..77574)
                     /locus_tag="IWJ07_000280"
     CDS             complement(74869..77574)
                     /locus_tag="IWJ07_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAP9331888.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(77880..78827)
                     /locus_tag="IWJ07_000281"
     CDS             complement(77880..78827)
                     /locus_tag="IWJ07_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAP9331889.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            79017..79286
                     /gene="rpsN"
                     /locus_tag="IWJ07_000282"
     CDS             79017..79286
                     /gene="rpsN"
                     /locus_tag="IWJ07_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP9331890.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(79462..79824)
                     /locus_tag="IWJ07_000283"
     CDS             complement(79462..79824)
                     /locus_tag="IWJ07_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP9331891.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(79835..80956)
                     /locus_tag="IWJ07_000284"
     CDS             complement(79835..80956)
                     /locus_tag="IWJ07_000284"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAP9331892.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(80972..81322)
                     /locus_tag="IWJ07_000285"
     CDS             complement(80972..81322)
                     /locus_tag="IWJ07_000285"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAP9331893.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(81452..82963)
                     /locus_tag="IWJ07_000286"
     CDS             complement(81452..82963)
                     /locus_tag="IWJ07_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP9331894.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(83285..84637)
                     /locus_tag="IWJ07_000287"
     CDS             complement(83285..84637)
                     /locus_tag="IWJ07_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAP9331895.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(84790..85866)
                     /locus_tag="IWJ07_000288"
     CDS             complement(84790..85866)
                     /locus_tag="IWJ07_000288"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAP9331896.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            86062..87807
                     /locus_tag="IWJ07_000289"
     CDS             86062..87807
                     /locus_tag="IWJ07_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAP9331897.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(88087..88524)
                     /locus_tag="IWJ07_000290"
     CDS             complement(88087..88524)
                     /locus_tag="IWJ07_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAP9331898.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(88508..89881)
                     /locus_tag="IWJ07_000291"
     CDS             complement(88508..89881)
                     /locus_tag="IWJ07_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9331899.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(90203..90691)
                     /locus_tag="IWJ07_000292"
     CDS             complement(90203..90691)
                     /locus_tag="IWJ07_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9331900.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(90713..91315)
                     /locus_tag="IWJ07_000293"
     CDS             complement(90713..91315)
                     /locus_tag="IWJ07_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP9331901.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        26642 a        19231 c        16359 g        29149 t
ORIGIN      
        1 tttcgctgtt attttcgatt atttatacat tttccgccta tcgtgccatt gacatattcc
       61 atcgtgcttc attgggagaa gctgtcgctt taacattttt gcctttgata ctaatggggt
      121 gttacgaaat ttatattaga gattaccaaa aatggtactg gttgtctatt ggcatgacgc
      181 tagtagtgta tactcattta ctttctgtcg ctatggtttc tgtatttatt ggaggaacgt
      241 tgtttctttc tttttatttt tgggaccaaa aaatcgctag actcttaagt cttttaaaag
      301 ctaccgtcct cacctttttt ttaagtgcgg gattcttaat tccttttatt caacaatcta
      361 gagcacaaga attgaaagtc ccactgggaa aagaattaag tggtatggct cctagtgata
      421 tgctgacaca tattttaaat aataactata acaactatac tattggatta ttcttatttc
      481 tcggattgat tggtgctttt atttttataa aaaaattaac tgctgatgat ttatttattt
      541 tctttcttgg tgtatttgtt cttttttgtt caaccaattt atttccttgg caacttttta
      601 accatacacc agttaagagt ctacaatttg tctggcggtt aaatggtttt tcttctttat
      661 ttatagccta tacgatgagt atagtgatct attatatttt ttctactaaa aataattcat
      721 ggaaaataat ggtctttacg tttcttgcac taatattgca tctctcagga gtaagcaatt
      781 ctatccatgc taacaaagat tcattaacac ttatagcacc tgaagacgct gttgctatag
      841 ctgaaaatta caatcatact gattatgcaa acaaagagtc aatctatcat cctgatatga
      901 ttaataatga tcaatattta ctaggtaacc aggaaataaa tccaacaact cattttatga
      961 gtaacaaact tacaattgaa ttagataatt cgaataataa aaatactgtc ttgacgacac
     1021 ctatttatcg ttataaaggg caagtggctt ctattaatgg caaactagta caaacaaaat
     1081 tatctaagtt tggcacaact gaattaacta ttcctccagg tataaataaa gtcgttatta
     1141 cttatcagta cacaaaacta gcaattgctt caagatattt atctatcgtt actttgattt
     1201 tatttttact ttatcgattt actttttcta aacaaaaaca accgagaaga gaagtacaac
     1261 attctcacta gatttttatt taagaaagac tttttagctg tacacataaa caggctaaaa
     1321 agtctttttt tatatttctt taatttgctt catacattat ttaacatcga tatttatact
     1381 gtgaaacatg cgtttcatcc attatcaaag tatttttatc gataataaac ttcatccaaa
     1441 aacagacctt gtgcagggac ggtttctcct gcatgttgcc gcactttgct ttcaaatatt
     1501 tcatcgattg tttctatcgg catggcacct gtgccgattt ctagtaatgt ccccatgata
     1561 atccgcacca ttttatataa gaatccgtct ccgacaaaag taaaatgaag catttctccc
     1621 tttcgttcga tagataattc atcaatcgta cgcactgtgg atttttttga ttttttcaat
     1681 gcagaaaaac caatgaaatc atgtgtaccc actaattttt cgcaagctgc attcagcttg
     1741 tccatatcca actcttgagg gaaataaaag ctgtggtttc gctcaaatgc tgaaggaatc
     1801 acgttgttcc aaacataata gctgtatttt ttgcccaccg cattatatct tgaatggaag
     1861 cgctcaggca tttcttcgat tcttttgatg acaatatccc gcggaaggta acgattcata
     1921 aagtcgatca tttctgccag tccaatttct gtgtttgttt taaaatttgc tacttgtcct
     1981 cgcgcatggg tgccagcatc tgttcttcct gatccaataa tttctatctc ttcatttgtc
     2041 atctgatgca atactttttc gatttttcct tgaatggttt tatcgtcatc tccaagtctt
     2101 tgccatcctg aatatctttt tccgtcatat tcgattgtca acttgatgtt tctcatattt
     2161 tagtggctcc tctgtcttcc atttggtctt ctctctacct ttttaagatt gtagcacaaa
     2221 atatagctcc ttgcttctct aagacagaag ctatacaaga aaactgtgga aatatatatt
     2281 tcagaaaaac aaaaaacagc caccggatga agtatccgct gactgttcaa tttgacggct
     2341 caagttttac tgtttgagat gcaagatact caaccaggca atgggcacat aattaaagaa
     2401 aaaactgatt ctttggcaaa aacctatttt attcagtaat ggccaagaat ttatagaagg
     2461 aattcttgcc agtccataaa tcgccgcaaa aagcaaactg atcacagaca gtccaaggta
     2521 taagtggctt acgtccattt gcccattttt tcgatagatc aagaaaagaa gaactggaaa
     2581 caataaaaaa ccactgtaac ctaaacctga tccgatatta tggacccatg tcgagacatt
     2641 ccacgaagat tcatgggtat tgatactgaa caaacctgta aagatacaat cacctatacc
     2701 atagaacgcg atggctgctg taactagggt agctaacaca cgagatgtac taacaaatgc
     2761 agcatacaat gcaggcaatg acaggatgaa aaagatccct gaaaccacag accaaataag
     2821 aaacgctttt cttacaggac tggaaacctc tccaaataca ctgatcacgc tcttcatttg
     2881 atctaattca ggataataca atcctaatac gtacggagtc aaaaaatcac tcagcacgcc
     2941 taataataaa aagaaaaaac caaatttacg taaaaaatcc atgatacgcc tccaataaca
     3001 attacctatg cttttaattg ttattataaa cgactttatc ttgatttaac agaaacctct
     3061 atttacacga acatctatat aactcattaa aaactcgaat cattttaaaa atactaaata
     3121 tctaacgttc aacgttcact taatccatct aaacaatctt tcaatttgat tttttcccca
     3181 cttcttttac gcgcgcctag ctctcctatt gcagtaaaca acagatcact tgacgaattt
     3241 aaggcagttt ccattgaatc ctggataaca ccgataataa atccaatacc gacgatttgc
     3301 atcgcgacat cattagagat accaaacaaa ctacatgcta atggaatcaa taacagtgaa
     3361 ccgcccgcaa cacctgaagc tccacaagca gctaaagcag acaaaataca taaaaggact
     3421 ttcaaaacaa aaggtgcttc aatccctaaa gaatgtacag cagtcaacgt catcaaggaa
     3481 atggtaatcg cggcaccgcc catattgatc gtagcaccta acgggatcga tatggaataa
     3541 gactcttttg tcaatttcaa tctttcagcc aacatcatgt ttatcgggat atttgcggca
     3601 gaacttcggg taaagaaagc gggaagtgcg ctttctttca aacaaaagaa aatcaatgga
     3661 tagggatttt ctttaataaa caaaaacgtg aggaatgggt acacaactaa tgcgacaaac
     3721 agcatcgtac cgatcaaaac aagtaaaagc tgcagatact ctgccaatcc agcgattcca
     3781 gtcgtagcaa ttgattgata aaccaatcct aaaatcccta tcggagcaaa tgcaataatc
     3841 acttgtacga cttgtgacaa cgctatagat atctgatcca ccaactcttt tgtcgattct
     3901 gaacgtgctc gaaaagctag tcccaacaat acagcccaga ataagacgcc aaggtaattc
     3961 gcatcaataa gagattgaac aggattttgt gtgacattcg tcaacatagt agataaaaca
     4021 ctttccaaac tttgcggtgc tttttcagat actgtttctt gtaaagtaat aggaacagta
     4081 aaaattgaac ttacaataac tgctacaagt gcagaaagca atgttcccac taaatataag
     4141 atcaaaatag gcttcacgaa tgtcttattc ccaattttat gcttggcaat tgacgccatc
     4201 gtcaagaaaa aaaccagaat aggtgcgatt gccttcaagc aactgacaaa caacgttccc
     4261 aatattgaaa taaatgtcca tgtggggaaa aagataccaa acaatattcc gactaaaata
     4321 ccaacaacaa tacgttgaac taacgacata tccaaaaact tctctaacaa atgaccaccc
     4381 tttcacaatt ttaacaatca ttgtatcatt ttatgcaaag aaactaaagg tataattttc
     4441 taaaaattct aacaatacaa acaaccattt cttgctaatt ttgtactttt tctgtctaca
     4501 cgttgttttt tgagatattc taagttagga tgttattaat gggcaactta tcaggaaacg
     4561 ttttcttatg tttttgtcca atttttttgt gaggtgttta ttcttgaaaa aaataaagaa
     4621 ggagagttat ggcttcctga caatctttgt cgcttgtata atgatggttt tacagcgtat
     4681 attgatttca gcagtcacta ttcctacata tgcaacaaac cctggtcgat tctacatcta
     4741 tacattttta caaacttccg ttgttattgg tgcaaatttg atcccacttt acatgggtta
     4801 ccaatatcaa aaaatcgccc gtttaaaagt tttacattat ttaagtcgtt ttgcctttat
     4861 attcctaata gccacactag tttgcaatat ctttttttac gtacaagctg gcagcttgaa
     4921 tattcgtgat tattggctta tcctaacacc tatcagtcag aattacttta cttatgctgt
     4981 ttcttgtact ttgctttttt gtagtattcc ttatacagtt ggatttttaa ataaattatc
     5041 gaaaacaaca attaaatatc tactattatt tttaacaatt ggattagttg gctgctctac
     5101 tctatttaat aaagacccgt ttgcattaca gaatggacag agtattttat ggatttttta
     5161 tttattttta atgggttatg gtttgaaaga atggaactgg aaagaaaagg tccgctataa
     5221 gcttcctcaa gtatttatca gcttgattgt tttattcggt ttgattattt tgatgacaca
     5281 aatttcactc atcatcagag gaaatacaga tacagcgctg aggttttcta aaccatattc
     5341 tctcttcgca atgtattata ctttctcttc gtttttattt ttagaaatgc tatctgataa
     5401 attaggtata aaaggaagag gaaattttct tgctgtattt ttgattgtca ctcaagttat
     5461 atgtaatttc cctttagttt cttatcgcgt atctacattt atgaaacgtg aatttccaaa
     5521 ctcagggaag gcatggctga taaatatcgg gcagtttttt atttgatatt tattagcagt
     5581 gtccctccta gttatctttt tattttatat acaaaaaatc cgtttcattc atcgtttcat
     5641 cgaatacttc gcttttgatt cgattgaaca attaactcag cgttggaaaa aaagaattca
     5701 ttggcttttt gttcataaaa gttatttttt agttgcagca tttttttact gctttacctt
     5761 cgtgcagatt ttcgttttag aaccaaaaga aggttggaaa gaaactatcc aggttgcctt
     5821 aaaaatattt actcagcgac aagcacccat gatcctgaca atcatcatta ttatgggatt
     5881 tttcttctta ctattgttga tttccaatcg tttttggtac gccttgactg ctaccttgat
     5941 cattaactta ctgctcacta tttcgacagt aatcaaaatg gaaatgcggg aagagcctgt
     6001 ctttccttca gatttaaaaa tgctgaccgg attgtcagaa ctattatcga tggttagccc
     6061 agtcttatta atcgtgggcg gattgatcct tctcctatta ttgattacta gtattattgt
     6121 acaacgtaga ctacaaaaac agtattcctt gaagattcat tggaaacgtc gatttatcag
     6181 tatcgctgta ttattaggat gtttttcggg tgtctttttc attaatcaca aaaattcgcc
     6241 ttctttcttg ctgtttaatt tatttaaagt aaacaagact ttctttaacc agcaaggcgc
     6301 cgtaaaagat aacggaccag tcattcaatt cctaaacaat atcgacatta aaatcatgga
     6361 agaacctgct ggctattcga aagaaaaaat taatggaata atgaaaaagt acaacacaaa
     6421 ggcagatgaa atcaaccaaa caagaagtga ctggttaaac aatcagacga tcgttctcaa
     6481 tttaagtgaa agcttcagtg atcctgcacg tgtccctaat ctgacagtac ctacagaccc
     6541 tattccttac atcaaaagca ttataacaga aggaacagga ggattgatgt tatccgtcgg
     6601 ttatggtggc ggaacagcta acatggaatg ggaaggactg acaggcttga gtatcagtaa
     6661 cctgtctgct tcgttagtta caccttatac ccagctagtg gagcgtcaga aaataagtcc
     6721 aaatattacc gatctattcg atgaaaaaat agccatccat ccttttacag ctgctcttta
     6781 tcgcagaaaa caagtgtttg ataaatttgg ttttgacagg ttctattatt tagacagtcc
     6841 ggataaatta acttatacgg ataaaataga acaaagccca cgcatatcgg atgattcagc
     6901 atacaaagaa accttgaaag ctttacaaag caatacggaa acgtcacaat tcatccaatt
     6961 atccactatg caaaaccata tgccttatga tgattactat agtgaattga actacactgc
     7021 tgaaggaact gctgtcattc ctaatagaag aaaagaatta caaacttata tgcaaggcct
     7081 tcactatacc gatacagcag taaaaaaatt catccaagaa atcgacaaaa tcgataaacc
     7141 gattaccttt gttttttatg gtgaccattt acccgcaatc tatagtggga atgattcgaa
     7201 aaaatatgga ttagaacagc accagacaga ttatttcatt tataacaaca agtatagtcg
     7261 tgaacaagca gctacgttaa ataaagaagt tgttgctcct aataactttg gtgctatggc
     7321 tcttgagcat gcgaatatca aagtaactcc ttactatgcg ttgttgacag aagtatctaa
     7381 tcatctacct gcattaacaa ttgatcctac agaaagcttg tctaatcgtt ttaatggaaa
     7441 acaggtcttt gttacagagg atagtcaaat tatccaagaa aaaaatttaa ctgagaaaca
     7501 aaaagaaatt ctagaagatt atcgtctgat acaatatgat cttgttgcag gtaaccagta
     7561 cagtgcaaca tgggcgacac aaaaaataaa agactaacaa ataaactgag caaggggttg
     7621 tgataaaaga catgtcacaa ccccttgttt atctcattaa tttgcttgtc cataaacgat
     7681 ttgattaaag ccatttttca attcattatt cgctgtctcg ttgaagtaaa gataattatc
     7741 gggattcccc tgattttgtt gtgtcaccaa tacttttggc tgattttgct gaaacccgaa
     7801 tagatagacg tgctttttca gatatggttc tgtctctgca tcggaataga cggctgtgat
     7861 ttgatagtct gcttgacctg tcccactttc tgaccattct gcagaaacag gcactccacc
     7921 aatagccatc ttccactccc cgttcaaaat agcttgaggg actttaagac catataagtc
     7981 cacactcatt tgattcgtat aacttttgta ttgttgtccc attgtttggc cccatttggt
     8041 cataaatgcc tctaattgtg cggatttgtc tgcattccac agtgtttgtt caacttgttc
     8101 gctgctttct gaagaggaag atgaagcctc tacagaagaa cttgaagtag atgtttgagt
     8161 tgtttcagtc gttttggaag actctgacga agtagaggta tctgctgttt ctgtacttgc
     8221 tgcagttgac ttgctctctg tgctagttgt tgtagtagtt gcaactgttg ctttcgctgt
     8281 ttcttttgtt gattcttttt tcgattcatt cccgttggta caaccagcta acaaaaaagc
     8341 tagaccaatt gttactaaaa tccttttttt caattaaaaa actcctttcg attactccca
     8401 cttctttatt ctatcaaata agcaatgtga ttttattgat ttttcgtatt ttctcatcaa
     8461 tctttcaaat cttctaaaaa ctgagaagca cccgctgatg ttgtctgtaa tacatcagcg
     8521 ggtgcttctt ttattttaat cttcgtgatt ttccaattta cctcacgctt tatatttgta
     8581 tttgaagaaa catagacttt tagtcatcat cgtcttcctc atccaaatct tgtcggacag
     8641 tttttccttg gagtctttgt acttggtaga tgatttcttt attgaaactt aaaagttcaa
     8701 caagcaattg ttgcaagcca tatttttctt ctttttcagc taatttgatt gcccgtgtga
     8761 taaagacatt ttggaaatca aaatcttttg caaagttttc caacattgtt gaggctggta
     8821 aatatttgtt ttgaccatgt tcgtaaattt ttccataacg catgacttct tctaaagtag
     8881 acggaacgat ttcattttca atcactaact catgagagat tttattgaac catctcgctt
     8941 cttgatcagc atattctgaa aaaagggtac ttaattcttg cgaagatgta cccaaaacat
     9001 acaattgtgc ttggatcaat ttcatttctt ctaatttaaa atttgctaat acatgcccca
     9061 ccatggcgcc agcagttggt ttatgatggt cgatatctgc ttgttttgtt tcttgaacaa
     9121 atttttcttc cactatattc attgatttac gcctctttta cttttgctga ttggacttca
     9181 taacctaatt tttccacaac tgcagctaat tgttcagggt ggacttcatc tggatcaaag
     9241 tttgctttga ttttgctcgc attaaacaaa actttcgtat tgcttacccc aggctgttga
     9301 gaaaccgcat gttcgatttt ttgcatacag cttggacaag ctagtgtatc taattgcata
     9361 ataatttttt tcatcgtagt tcttccttcc tttttcttga tagtcttatt ctagattaca
     9421 gcttccataa aaaaattgat tcttatcaag ttttacgatt tttcttcttt gctttcgtgt
     9481 ccaaaataca ataaacgcat cgcgttaaag ataacgatca agatactagc ttcgtgaacg
     9541 aacatcccac tagccatgta aataaacccg gcaaataagc cgttgagtaa aaataagacg
     9601 acaccaatgg caatcactac attttcacgg gtatttaaaa cagttttctt agccaagcgg
     9661 taggcatgaa ccaacgattc aaaattagat tgcatcaaga caacgtcact cgtttcgata
     9721 gcaacatccg ttcctgatcc catagcaatc ccgatatcag ccgcagccaa agagggagaa
     9781 tcattgattc catctccaat aaaagcaacg cgcaatcctt gttcttggta ttttttcaca
     9841 aattgaacct tttcgtctgg taataattca gcatggactt catcaatgcc taacatatca
     9901 gctacataat cagcagtcat ctgattatca cctgtcagca tcactaactg ttttgctcct
     9961 gcttgtttca acttagctaa ttgttcagcg acttctggtc gaatcacatc tgacacacct
    10021 atgatttgag tgattttttt gtcgattgct acaataatga ccgagctgcc tgtttgttgt
    10081 aattgatgta aatcttgtac ttgctttggt gtgagagaaa gtttgtgagc ggcaatcaat
    10141 ttggtgttgc ccgcacaaac tgtatgacca tcaatctcag ctgtgattcc ttgtcccttg
    10201 attgtctgat tatctttgac tgctaattgt tgaaaatcaa tgtctttctc ctgagcataa
    10261 tggatcacgg cgcgccccag aggatgatcg gataacgttt ctaatcgagc cactaaagct
    10321 aagctgtccc cttcatcctc tgtatagttc ttgatagtgg atacagcagt agttcctttg
    10381 gtcaatgtac cagttttatc aaagacaaaa gtatcgattt tggaaaaagt atccattgct
    10441 tcaccgcctt tgactaagat gccccgtttc gctccattac cgattcctgc tacattcgaa
    10501 acaggcgcac caatgactaa agcgccagga caaccaagaa ccaaaacagt gattgctaaa
    10561 cgaaaatcac gactaatcag accaacaacc aacgcaataa gtaaaacggt tggagtataa
    10621 tactgagaaa atcgatcaat gaatttttcc gcatgggatt tggtatcttg tgcttcttct
    10681 actaattcga taattttggc aaaggtcgta tcatctccga ccttagttgc ttcaatcgtc
    10741 acgtagccat ctgctaaaat cgttccagag aagacttcat ctccactgat tttgacgact
    10801 tgtcgtgatt caccagtcac tgcggcttca tccgcatacc ctttcccgtc aatgactcgt
    10861 ccgtccacag gaatagctcc accagttttt actaagacga tatcgccttc ttccacgtca
    10921 tcgatttcca cttcttcagt cgttccatct tcttggacaa cactagcagt agatggggcc
    10981 atctcggtca aagccttgat tgactcccgc gttttggtca atgttttttg ttccagatag
    11041 ctaccaaaca aaaacaagaa tgtaacgata gctgactcct tgtattctcc gatcataaag
    11101 gcaccaagca cagcaatagt aacgagtaaa tcaattgaga taactttgac tttcactgcc
    11161 tgatacgcat ggatcataat aggtatgacc gcaataatag aagctatggc tagagcaatg
    11221 ttatagaaca agacaaaacc agtaaatttt ccgataaaac caaaaataat caacacagct
    11281 gataaggcgg tgatatgatt tcgatgctct tggatccact tttgtaattt catctttctc
    11341 cctcttttcc tttcattctt acgagactaa gtatagatac ttatccatca taaaaaattg
    11401 accactatca agtttagtca aaaaggcggt tttcttttgg tagaatacac tacaaaaaat
    11461 agttagcaag aagactcact aaaagtgatc ttgccaacta ttgtcgtttc tatcgaaatt
    11521 ttcattgaat gattttcctg tacagcatac atcggagatc tatttcttta tctaagattt
    11581 ttatcttgct aattttttcc tttagtcttt caaatccacg tcgttcatag aattgatacg
    11641 tacagccact atctgtaaaa aggaatattt cttttccttt ctctcttttt tcaaattctg
    11701 ttagcaactt ggtaccgatg ccttttgttt tcaaatcagg atttgcagct aaaaaaatga
    11761 tctctccatc tggcgaatgc ttctttaaat atgctgcaag catttgttca tttgctgtct
    11821 cgtaagctcc tttactttct ttagcaaata tattttgtaa aaaatcgaat atcttaacat
    11881 atatttgctg tccaaaagaa cgatgtttct ttttttctcc ttttatctcc gccagcaaaa
    11941 cacctgccaa ctcgtcctct gcatacaaag caagaatttg agttgctcga ttcaactcca
    12001 tatacaaaaa atatcttccg taaagattca gcagaagttc attttccata tatctctcaa
    12061 agttcattcc ttcaacagca aaatagatag cttttttgta atctttcttt tctaagggtt
    12121 taaaagttag tttcatcatt tcgcctcctc aacttcaatg attgtgttat acttacatta
    12181 taatagacac agtgtctact atctataatc aatcatctat aagtttgtcg attaagcgac
    12241 agctttgctt tgatatgtcg gataaggaga taggtaaatg aaaaagacag atagacgcgt
    12301 caaaaaaaca gaaaaagctt tggctgaaac actatccaca ttattagtaa ataaaaagat
    12361 tcaagcaatc actatacgcg aactaacaga aacggcagat gtacatcgtt cgacttttta
    12421 tacacattat aaggatatct atgatctata tgatcaatta gaaagcaact tttttcggga
    12481 tctaaatgaa attctctctt atgatccagc ccatagctat gaggaactat acactcgctt
    12541 gattgactat gtttacgcaa atactttttt atatcgctta tttatcctca accatggtga
    12601 tatttcgttt cagaagaaag ttaaacaagt tattgaagaa aactatctga acatatggct
    12661 atatgaagac aagaaaacga ttatcacaga agaaatgaga ttctttacta cgtaccacgt
    12721 ccaaggttgt ctatccgttc tcaatcggtg ggtagaaagc aattacaagt actccaaaaa
    12781 agaattatta gaactcttac agaaactgaa tgatcacata gagaaaatca tgccttgaat
    12841 aaaaacatac taagcttagt taagaagaac cgtaaccttc tcgtataaca actgaatctt
    12901 atttagccaa attgtttact gaagattttt tgagtaaaat ttctataatt cctacttatt
    12961 gaaaagtagt ttgtagtgct gcttctccct ctctaagtga tatatcttgt tgtaccaatc
    13021 aaattcacct catcattaat aaattacttt taggctaatt tattacaact tataggttga
    13081 agctgtaacc aatttatagt catattatat ttttatcatc cagtttattg aattcttctc
    13141 gtttacctca attcattttc aaggggaatc agctcatcat ctaacatttt acgccatgct
    13201 cgcttcattt cttgtgggta tttaaatggt ttcgcttctg gatgtttggc tttatatcct
    13261 tcgtaatcct tactgttgat aattgctttc atattaggaa ttggatcttc acctggcata
    13321 tattgcagat tagaagcatg taattttttc tcagaaacaa accactcatt ggaaaagaca
    13381 tgaatcaaat catcacttac ttgtgtaaaa tagcgacgtt taatgacaaa gatatcttca
    13441 ttgctatcta gtgtaccttc atttctttgt gcttaaattc aaagataatt ttctcaataa
    13501 agatttttga ttccagtaag attcctttac tccttttaca acatagcgtt gtcctttgtt
    13561 ttgaaacatc ttcttcccct ctttgccatt aaaaatagcc caattctcta taaagaagat
    13621 taagcttcat tattataata taaagtttaa atttggttta ttacttactt tttcttattc
    13681 tctagtaagc taatattgaa aaaaatgtaa tcagatcatt atttttatat gttgtaactt
    13741 cctgataaga aaactaatta aaagaaagag gtaactacat aaactaatta aaaaagctaa
    13801 cttcatagtt ggaggataaa tggaagaaac ttcaatgatt gtttctcctt gtgttccttc
    13861 aatcgacact agtcctatta tcaatctctc ttttttgtcc atttactttt gcgactgttc
    13921 cccaataatc aaatacagga agcgttattt tttgtccttt agattcatgt actgtataga
    13981 ctattttcga atccatcact tgataaggca tagtatgcca ttccttgcca attcttactt
    14041 catgattctg caaatgttct ttgtattgta atgcttccgt gggtgcatag tcataaagtc
    14101 ctccgggaag gagtgtcgtg taacgaataa gattttcttg attcattacc aagtgatctc
    14161 tctgagtgac aactttatgt tcatagtttg cttttgttgc agcagataca gccaacagta
    14221 ataaaacaat tgccgttata ttaactactt ttctttgctt agctgatgtg ctcttcatga
    14281 aaacgaacga taaaatcaac gagccaaaca gtacttgaaa gcctaagatt cgccaaggaa
    14341 attgaatcat ttgaatgggt gtattttgta ataaagacca tggaaaaaat ttggtggata
    14401 agagccaaga gactaccgca ctaaggtaaa tcactttttc tttgcgatta aatcctttaa
    14461 aaaagaagag tggacagatc aaagaaataa taccaatgat tcctaccgta aaggttgtca
    14521 ggtcattatt catcgcacca atcaaactat ccgagacatt taatgcttgg tgttgtaagg
    14581 tcgttttatc tggctcatta atcgattgaa ccatgatttg tcggaacatc ggataccaaa
    14641 agtatgctgt taaaagaatc gtcattccta ctgcctttga caactccaca atcaaccgga
    14701 tgctttcttt ttttcttgtt tcacttacta caaagccgcc taagactagt aagccaacgg
    14761 ctattacact cataaaagtg gaaagaacat gtgtataaat gagtaagctc atactgactg
    14821 ctaatggaat tgcacatggc tttttctcat aaatcagtgc gtaagcataa tacataacta
    14881 tcggtataac cgtaagtgca atcgcttcag caatagcaga acgatgataa atatcaatcg
    14941 tacggtaccc ataaaatgta tataaacagc taaatataaa agccgcatcg attcttttta
    15001 aaaattttag tccataatga aacatcagca tgatagtaca aacattcaat agccaaacat
    15061 ataagatata actaacgatt aaattattac ttatccaata aaaaatgact gccggaaaaa
    15121 aggtcaagta gggataaaaa taattcactc cgctcccata atgattaaac gtcgtaaaat
    15181 tgattggacc agaaaaaatt gaagataacc ctttgactct agcaaaatgg aaggcaatat
    15241 cccccgatgt agaaatagta tgaacatttc taaaataatt gattgaataa agtccagcaa
    15301 taattgctag gagaatggac aatattattt ttttctttac ttgcattact taattatata
    15361 ctcctttcat ggattgcttt atctagtata acttaaattt taaataaatc gttatttatt
    15421 tttactctca cttaaaaaag cactaaaatt ataagagagg gaggtaatgg tctgaacacc
    15481 aagaattgtt ccaacatcga attgcttaac taatgagttc aaagcttaat tatgtggctc
    15541 ttatatttac cgagcgtgga ttgcgatctc tttagcgaaa caaacatctt tcataaaaaa
    15601 aacagccata tccgctttct tggatatgac tatttactac tactttacta tctttcattc
    15661 ttcttgtctc acttatgata aggcgccccc gcattgatcc ggaaggcacg gtagatttgc
    15721 tcgacaagga tcagacgcat caattgatgt ggatacgtca tttttccaaa agagatctga
    15781 gcattgcttc ttttcatgac ggcttcgctg agtcctaaag agccgccgat aacaaaaaca
    15841 aggtggcttt ttccttggat acctaattgg tcgatttgtt tagcaaaaga ttcacttgta
    15901 ggatttttcc cttcaattgc taaggcatag acgtattctt gttctttgat tttcgctaag
    15961 atccgttccc cttctttttc ttttacttgt ctcatttgcg cttcgcttaa attctccggg
    16021 gctttttcgt caggaacttc gatcaattcg atttttgcat aggcattcag ccgtttgaca
    16081 tactcattga taccttggat caggtatttt tcttttaatt ttcctactga gataattttg
    16141 atattcatgt tttctctcct ttggtcttca tgctcacttt gtttattata atcaattttt
    16201 tcgtctatat gtttattttg gctatttttg ttaaaaagat ttccccaaca gttatgcaca
    16261 acatccacag gtttatccac agaaaacagt gttattttac cttatttcat cacattcatt
    16321 aggcaaacat acgttttata agaatttatc cactttatac acaggctgtg gatatgtttt
    16381 atcccttatt tttccaatcc tatccgcgta gttttacaca gtatctgtgg aaaaagaaaa
    16441 tcaaatattt tcatctgaaa acaatgattt ttcctaaagt ttttcataaa aagttcacag
    16501 tctcaaagta gactaaagtc aacaaataag actatactaa cagtagataa atgaatgtaa
    16561 ttggaggagg aacacatgga tagaaaaaat gtgacaccaa agatgaaaaa aagtaaaaat
    16621 aatagcctct ggcgtaagtt aggtctgggg cttgttggag ggatcgttgg aggactactg
    16681 acagctggca ttttttatgc agcgatgggc actggcaaca atagttctac tgctacaagc
    16741 ggcaatcaga attctgccgg tgaaacagtt gtggaaaatg tcaaagtcaa tgtagattct
    16801 gatatcacca aagcggtaga taaagttcaa gacgctgtcg tttctgttat caacttacaa
    16861 agtcaaaacc aatcaagcgg ttttggcgga ctattcgggc agcaagaaga aagtagcagc
    16921 tcagatgata gtaatttgga agcttatagt gaaggaagcg gcgtgatcta caaaaaagac
    16981 gggaatactg cttatgtagt cacgaataat cacgtagtag atggccagca aggtcttgaa
    17041 gtcttgatga aagacggtac gaaggtaaaa gctgaattag ttggaacaga tgcttattca
    17101 gatttagcgg tgttgaagat caattcagac aaagtcgaaa cagttgcctc ttttggtgac
    17161 tcaagtgctc tgaaggttgg tgagcctgcg attgcgattg gttccccact aggatctgaa
    17221 tatgcaaatt ctgtcacttc aggaatcatt tcttccttga accgacaagt gacaagtacg
    17281 aatgaatcga acgaaacagt caatatcaat gcgatccaaa cagatgcagc aatcaaccct
    17341 ggaaattctg gtggtccttt agttaatatc gaaggacaag ttatcggtat caactcaagt
    17401 aagatcgcaa gtacttctga atctacttct aatgtaagcg tagaaggaat gggatttgct
    17461 attccaagta acgatgtagt gaatatcatc aatcaactgg aaaaagacgg aaaagtgaca
    17521 cgtccagctc taggtatcac aatggtcgac ttatctgccg tttctactca gcaacaagaa
    17581 caaatcttga agattccttc atctgtaact aacggtgtca tcgtgacaaa tgtccaaaca
    17641 gctacaccgg ctgaaaaagc tggattgaaa caatacgacg tcattactaa gatcgatgac
    17701 aaagaagttt cttctggtgt agaacttcaa tctgctttgt ataagaaaaa agtgggagat
    17761 tctgtcaaaa tcacttacta tgaaggctcg aaagaaaaaa caactactgt tgaattgacg
    17821 atcgatcaat cggcattaaa acaaaatcaa aatagtgaaa attaataaaa aagaggttgt
    17881 gacaaagact tgtcacaacc tctttatttt ggaaatgctt ctccgactac ggcatcctca
    17941 ggcaatacta agattccttt ttttgctgga gcatttggca aatgcaattc acgagcggaa
    18001 cagatcattc catagctatc tacccctcgc aaagttcctg gccagatcat cattccatct
    18061 ggcatcatag ctcctggttt tgcaacgaca actttttgtc cagcttcgat attgggtgca
    18121 ccgcaaacga tttgcaatac tgtaccattg tctacttctg tctcagtaat cgataaatgg
    18181 tcagaatcag gatgtgggac gcatgttttt acgtacccga caacgatctt cggttcatga
    18241 tcagcttcaa gctgttcttc gaatcctgct tctttgatca atcgattcaa tttcgctaat
    18301 tcctcatctg ttaagcttac ttggccgttc ccttcgattg ttagatgatc ggagatatgg
    18361 aaaaagttcc aagcaacaac ttgaccttgt tcatctttga tccgagcaac attccatttg
    18421 cgttcaacag tgttttctgt tcctttatcg tcggctataa taaccatcaa ggtgtctccg
    18481 acgtgtgctt tgttataagc aaaaatcatg ttctttcctc cattatcctt tgctgagatt
    18541 tatcttaaca aaaaattgca ttcactgcat gaaatttttt tcatgcttta tttccgaaga
    18601 agcctcttct ggaacaccat ttattcgtaa caagggactg tgacaagact tttgtcacag
    18661 tccctttact gttttagtta attgattaaa aaggtcgtac aagttccact tgcttaatta
    18721 cttggctgtt cttcttcgtc agaaacgatc aagacatcac aaggtgaatg acgtatcacg
    18781 tagctggcta cacttcctgt cataaagcgt tcgactgcat ttagacctga ctgcccaacc
    18841 atgattagat cgatctcata tttttttggc aatttgactg ccatggattc acgaacagaa
    18901 ccatatgcaa taattccttc tatatttgtg taatcaacag atttcccata ttctttacat
    18961 tcttcgatca tttccttggc tgtttcagtt tgctgatcga ctagattttg atcaataggt
    19021 gcatagccca taaagttata aaattgctga tcgattacgt ttgctacata tactttccca
    19081 ttattacggc gagccacttc taccgctttt ttgaaagcag caagtgcttg ttcactaccg
    19141 tcgataccta ctaaaatatt cttatattct tgattcatct tgacccctcc taacagatgt
    19201 cagccttttg ttacactttc taggaacgat tcgatttcct gttttgtttt acgatcttta
    19261 ttcactagac gacctaattc ttttccgtct tggatcacga caaaacttgg gataccaaaa
    19321 atgttccatt cagcggctac atctatgtac ttgtcacggt ctacttcaat aaattgccaa
    19381 tcagaaaagt cattttcaat ttcaggcatt tgcggtttga taaaacggca gtctccgcac
    19441 cagccggcag taaagaaaaa tacactcttt ccagtttcta cgtagcctgc taattcttct
    19501 aaactatttg ggataatcat tctttgtcct ctactttctg tttatgtatt tttcaattaa
    19561 attatacacc tttctttcga actaacaaac attgatcctc tttttataaa aaaaatctgg
    19621 caaaaagcaa tcttttgcca gattttatag tttcactatc gcattatttt ccgtatacga
    19681 tcgtatttac tgttgattta tccaaacctt tcagtgtttg aatcaacatt tctcgtgcag
    19741 cttcaaaatc gcgaatactg aacatcgttt gatgcgtatg gatataacgt cccactacgc
    19801 caatcactgt acttgggatt ccttcgtttt gtgtatgagc agcacctgca tctgtaccgc
    19861 cttttgaaac aaagtattga taagggatat tgtttgtttc agcgatatcc agcaagtact
    19921 cgcgcaaacg aggaagcatg atcaaacctg gatcttggat acgcatcaat gtaccttctc
    19981 ctaaatgtcc gaatgtgcca tttttcgtca ctgtatcatc tgctgcagag cagtctactg
    20041 caaagaacag atctggcttg aatttattga cagaagcttt agaaccacgc aagcctactt
    20101 cttcctggac attcgcgcct gcaatcaatg tatgacctaa tgtttcattt tgcaacgctt
    20161 ctaaagcttc taataccatt gtacagccat agcggttgtc ccatgattta ctgataatat
    20221 tctttccgtt ggctgttttg atcgtttcag cgtaaggaac gattgtgtct cctggaagga
    20281 caccgtaact cattgcctct tctcttgatt caaacccagc atcgaacaaa atatcggtta
    20341 cttctactga tttttgtccg cttgttcctc gcaataaatg tgggggaacg gaagaagaaa
    20401 tacatggata attcttacca gtagaggttt taagtgtaaa acgctgagca gaaacaacgt
    20461 aaggattcca gccgcctaga ggtacgactt taaaaagccc gttttcttgg atttgtgtca
    20521 gcatgaatcc gacttcatcc atatgtgctg cgaccattac acggggtgca tccgcttctt
    20581 catgatggcg cagaccaaaa atgccgccca aaccatctaa ctgtaactca tctaccagcg
    20641 gttccatatg actttccata taagcgcgaa catcttgttc aaaaccgctt gttccttgta
    20701 attctgttaa ctctttgatt cgttgaaatg ttttttcttc catatgctat tcgtctaaat
    20761 ttcagactaa actcctctca gacttatatt gatctgcgga taaaatatga ttttttctgc
    20821 agaccgatcg tatttattat agcttatttt ttctggtagc ggttatttat ttcaaagaaa
    20881 atatgagagt gtggtaaaat aagcacaagg agtaacaagt gagaagaagg aggtatgtcc
    20941 acatgtatga agaaagcgaa ctaagctatt ttaaaggcgg gttagcagtt ggaatcggtc
    21001 taggattatt gagcggggta gcttcaacga tttggtatca tcgcaaaaaa gtattgtcag
    21061 cagaccaagt acttgaaaat atcaagtcat cttttctaaa agaaggtgca atcgaaggtt
    21121 cttggatctc ttttgaaaag gaacctatta ggaaattcgc cgttcggtcg agtgcttatc
    21181 ggggaggtct ttctcgaata gaagatggcg aaactgttta ttatgaattt ttagcagatg
    21241 ctcatacagg aaccgtatta gaaatttcaa gaaaaaaagg tgcaacagat taatacgtca
    21301 aaaaagtgag agaacgaata cgcacccact attcaaaaga gaggtatcat gtccttcttt
    21361 tgtagtgggt ggttttttag atagagcttt ccaaacatat attccagtat tttcttttta
    21421 aataaatgat atttagcggg aaaaactagc atttcctttg attatgaggt atttttaatt
    21481 tagcttttgt tttctatagt taaggagagt gaaaaatgga acaacaagaa aaaattgctg
    21541 ttctgattcc ttgttacaat gaggaagcaa caatttcaac agtcattgca gactttaaac
    21601 gagaattacc agaagcagat atttatgtct atgataataa ttcgaccgat cgaacatacg
    21661 aattggccgt tcaaggtgga gcaatcgtaa aaaaagaacc tcgtcaagga aaaggaaatg
    21721 tgatccgcca aatgttcttt gatatcgatg ccgactatta tttaatggta gatggcgatg
    21781 atacttatcc tgcagaagct gtacatggtt tgctcgagaa attacgttct ggtgaagcag
    21841 atatggtcat tggtgatcgc ctatcaaatg gaacctactt tgatgagaat aagagacctt
    21901 tccacgattt tggaaataac cttgttagaa atacaattac tagaatgtat aaaacaaaaa
    21961 ttcttgatgt catgaccggc tatcgaggat tcaatcgtat ctttgtcaaa agttttccta
    22021 ttatgagttc tggttttcaa atcgaaacgg aattgacgat ccatgcttta gataaaaaat
    22081 tcaagttcgt tgaaatccca atcgattatc gtgatcgtcc tgaaggcagc gaatcaaaat
    22141 tgaatacatt ctctgacgga ttcaaagtga tcatgatgat tattaaaatg tttaaagatt
    22201 ataagccttt gcttttcttt gggatttgga ccatcttctt ctttttattt ggattggtca
    22261 cggggattcc tgtgattcgc gaatatatgc tgacaagctt tattacacgc atcccttctg
    22321 cgattttaag tactggattg atgactttag cacttctttc attagtaaca gggttgattt
    22381 tggatacagt cgtcacgaac gctaagaaag aatatgaatt gaatctatat catgtttatc
    22441 atgaatacca cagtagaaag taaaaaaagg atcaagactg caaaaataat ttgcaattct
    22501 tgatcctctt tttaattaat tatttcgcca aacgatagat ggcatctgca tagattgctg
    22561 ttgctctgaa caagtcatca agactcataa attcgtttgc ttgatgcatt gtatcagtat
    22621 atcctgggaa catcgctcca taagcaacgc cacgtttcaa caaacgtccg taagttccgc
    22681 caccgatgat ttgttcgtag cctttttctc cagtgtggtc ttcatagact tgcaataatg
    22741 tagccaccaa tggatcatcc attggtacgt aatgtggttc cattacgcga gcacctcgag
    22801 taactgttgc gccttcttgc cccactgttt gttcgatgtc actttgcaat ccgtctactg
    22861 ttacaccttt tggaaaacgg aaattcatat tgatgaagtt cgcttcctct gtaccatttt
    22921 cttcaaaagc aaatagtcca gcattcattg tcaatttgcc cattttttca tcttcataag
    22981 cgactcctag tttttctcca taaaaatctt catggacata agcagctgct acatggataa
    23041 attgtttagc agatcctagg aattcatagt tatctaagaa caatgccaag aaagaacctg
    23101 cattgatacc agattgtggg cttgcaccat gtgcgccttt accaactaat tcgattttta
    23161 catacgtatt gtccgcttca atcgttccgc ttactggatt tgcttcgatg aattggtaaa
    23221 atgcttcttc catcgcgata gctgcatctg cacttggtac ttgaagtgct gcagttgctg
    23281 ttcctggcac catattttcg cgaagtccgg aaacaaaaga tttcaagaca taatctcctg
    23341 cattgtcgcc tttgaagcgt aaagctaaag aaacatttcc tttttcacca ttgatgattg
    23401 ggaattcagc atctggtgag aaaccaaaat ctggttcttc ttcatgttcg aaataataag
    23461 ccatatcgcc ccagccgctt tcttcatcgc tgccgactac gaagcgaact ttttttgata
    23521 gtttcaagtc aagttcttta atgatcttca tcgcatagta agctgccata cttggacctt
    23581 tgtcatcgct tgatccgcgt gcaaagatct ttccatcctt gattactggt tcataaggat
    23641 ctgtgtccca gccatctcct gcaggaacga catccatgtg tccaaatatc cctagcgttt
    23701 cgtctccttc gccaagatca atatgccctg catagttatc gacatttttt actttaaatc
    23761 catcacgttc gccataagct aacatatgtt tcaatgcgtc gcgaggtcca ggtccaaatg
    23821 gtgcatctgg tgttactttt gagtcatcgc gctcgctgtt gacacgtaaa agatttttca
    23881 agtcttcaag caagtcttct ttacgtgctt ctacttcttt ttgccaatca attgtcataa
    23941 agttaatcct ccacttattt aagtcttcgt gttcatgata ccacaaatta cagaaaaaaa
    24001 tagttcaaaa aatctgacaa ttccttttct tatctgtttt tctctaaaaa aaatatacgc
    24061 tataatgagt aaaaggaggt ttttagatgc tgataaacga acaactagtg aaaagagtca
    24121 tcacacctcg tccaaaaatt tcacataaag gaacatttgg acgtatcgtt ttgattggcg
    24181 gcaatgaaca atatggcgga gcaatcatta tgagtgcttt agctgccgta cacagcggag
    24241 cgggtctcac gactgtagta actgatgaaa aaaatcggag tgctcttcac agtcatctgc
    24301 cagaagcaat gattgtagtt tggacggata ctgaccagtt tctttccgta ataaagcaag
    24361 cagatgtact cttgattggt cccggattgg gtacttcttc taagagtttg gaacgtttca
    24421 aacaagtact agaaaaccaa tcaaaagaac aatggctggt gattgatggc tctgctttaa
    24481 gtttacttgc tgctaatcat cttttgctac cctatcccga acaaactgta cttacacctc
    24541 atcaaatgga gtggcaacgg ttgagttctc taccgataca agaacaacgg gataaagaaa
    24601 acaaaaagaa gcaacaggaa atcggtgctg tgctcgtttt gaaaagtcat gagacaaaaa
    24661 tttatggtca ccagcacttc tacaccaatc ctttaggttc tgctgctatg gcaacgggcg
    24721 gcatgggtga cactttagct gggatgatca caggattttt agcccagttt cctcaaaatg
    24781 accaaacgat tgcagcagct atttatcttc atagtttcat tggagaccag ctggcaaaag
    24841 aacaatatat tgttttgccg acacaaatca gcgaagcact tccgaaatgg atgaattatt
    24901 tttgtaaaaa atcaatggaa aacgaggcat gagtcatgga gaaatttaca ggttatgtgc
    24961 gagaaccttt ttattatgaa acagatcaaa tggggattat tcatcacagc aattatatcc
    25021 gctggtttga agaggctcgg gtagccttat tggtacatct agggtttccc tataaaaaaa
    25081 tcgaagaatc tgggatcatc atccccgttc tggaagtaaa ttgcagctat aaagaaatga
    25141 tccattacgg agaaaaagtg agcatccatc caactatcca aaagtatacg gggacacggt
    25201 tggattttag ctacgaaatt gtgggaactg aagacaaaaa attaaaaact actggcagta
    25261 gtaaacactg ttttttactt tctgattcgc aacgattagt gaagctgagc aaagtcaatc
    25321 cggagttaga tcagttattc caagcgtacg cttctttgga tgatgtaaat tcttaataaa
    25381 gaccgtctaa gcatttttct tgtattgtgt cgagtagttc gtataatgaa aagaaaaaag
    25441 caggtgatcc ccatgtatgt cgttaaagta atgcacggtt acatcgataa aactggctgt
    25501 cgcactcggg aaaaaaactt agacaatctg ctcattttca aagacaaaaa agaatccgaa
    25561 gcttttgcta aaagaatcgg cggtcgtgtc aaaccgatcc aagaggtacg accagattga
    25621 ttcaatattt ttcttttcga ggctctaata ggcatgccta tcagggcttt ttttgcagac
    25681 tgtgttttta tgcccacacg atttgtaagc gatttatgtt ataatagatt atatctgcat
    25741 agaaaggaag gttaaatggt tatcaataat ttgcaacagc aagcttatga atctatacgt
    25801 aaacaaatca tttattccga gttagcacct ggaagtaaaa tctccgataa agtgttagaa
    25861 gaacaactag ctatcaggcg tacaccgatt cgagaagcac tgatccaact gcgcaatcaa
    25921 gagcttgtaa aaaccattcc acagtccggc acttatgttt caaaaatcga tatccgatct
    25981 gcttcattag cccgttatac gcgggaaaag ttggaaaaac cgatcctgca agaatgcagt
    26041 gcgaaaatga ccaagcaaga tcaagctaaa ctagaaaaaa tactagaaca aaccgatcag
    26101 gcagtaatcg ctcaaaataa aaaagctttt tttgatttgg atactgattt tcaccgaact
    26161 tgttatgaaa ttgctggaaa acgagaaatt tgggattggc tagaaagtta tagtacgcac
    26221 ttgaaccgtt tcagatggtt acgtttgacg atttcagaat tagactgggg tcgagtatta
    26281 gatgaacatc agacaatgct tcaatcaatg attgatcata actttgatga agtcggattt
    26341 ctttgcacga tgcatttaca tatgattatc gaggagcaag aatacgtgat tcataattac
    26401 cccgattatt ttgaagatat tcaggacaga cccatcaaat aagaggtttt ggaatcagcg
    26461 ttttgacctg agtattagag aaagaaacgg caaaatagtt cctcatattt tgccgttttt
    26521 tttgagctaa taccgagggt cagctgattg cacaccgttt attgagaggt tgtgaaaaag
    26581 ctgccctgca tcaagtaata agaacagcca agtaaaaata gcttcttatg tttttaactt
    26641 ggctggactt attaccgcag atgcaagctt ttgaacaccg ttttccaaga ggtcgtgagc
    26701 ctgccgttca tctccgagtc acaaggaaca atttcaaaaa acagcttctc atgtttgttg
    26761 aatattttga cttgtgaccg aggagttgac aggagaacac cgtttattcg gaaaaaaagg
    26821 actgtgacac gacttttgtc acagtctctt tatttgtaat ctaaatcata ctccgtacat
    26881 cttttttcta ttttttttat ttcttctaag atcccttggc tacgaatttc attatatggc
    26941 caaagtaaga ctgcctctaa tacataaggc gaaaaatcgc tttgattcat ctcttttgaa
    27001 aaactcatcg ctgctatctc gctcaatgca cgaatgtttg attgatgtgt ataaaaaggc
    27061 aaccgccaca gcacaatttt ttcgctttgc gtataaattc ccttaacaga agcttcgatg
    27121 tggtgaaata gttcatgtcc aataatcaaa gcttgcgctg tttctttaga gatcgaagct
    27181 agtcctgaac attttaattt ttctaacggc tccgtcatca atcgaatccc ttcatcttct
    27241 gcataagttg caaaagtgat tcgttcgttt atttgctctg attcacagga aacgattgga
    27301 acagccattt gttcacaaat ttctcctaca tcggcaaaag ggtacttttt tttgatgcta
    27361 catgccgttt cgataccaca atcgattgat tgttgtataa gaattttctg cttggacaaa
    27421 ggaatctttt tccaaaataa atctcgccga aactggtaga atccccaaat ttcttcgggg
    27481 atctgttgta attcgtttag taactcttct aacttatgtt tgtttgatat ttccggcaac
    27541 gacgatcact tcttcctccg gttccatagt agatacttcc agatccatca aaagctgtag
    27601 ttgttccaga tccgtcgctg taaaatcaga gactcttgct cctaagcaga gataaaattc
    27661 tggtcgggca atcaattcag tttgataaac tgccatatct tcccaaagtt gttcgacagc
    27721 tgtcatataa ttggctgcag ttgttttcat acatgaagca tgtccacgtt ggaccttgat
    27781 aaatcctttt tgatcgatga tccgaagact tccagcattt tttgaacgat tctgttcgcc
    27841 gcatacataa aagaaaggtg tattttccaa taagcaaact tcatttttat ttaatcgcat
    27901 atcctctgca gcaagctcta gtgcttcttc tgttgtgatc tccttggtta gatcggttgc
    27961 tttcacttcc gtagaaccag tagcgatagc cgttactttt gaagtctgtg gatcgatttc
    28021 tacgtggact tcgatcgatt ctggtgtagc gcctgattca atcgctttat tcatcgcttc
    28081 atttttcaac gatcgtatat cttctttact tggcgaaggg atgatacgtt ctacaacatc
    28141 tcggaccatt gcaagtgcta caccaataga agaaatcact tcggcgtttt caggaatcga
    28201 atatttcaca cccattttat tactaaagta agtaatcaat gaggcagcgc cgccccctac
    28261 acctacaaga gaaatctgat ctttctccaa gtgatacttg tctgccaatt ctaaaatcac
    28321 cggttcaatc ttagcatagg atttttccat gatctgttct gcgatgtctt caaccgtcgt
    28381 atgacaataa tcagctaatg cttggatggc ttttcgtgca gatgggacat ttccataaga
    28441 aaaatgttct tcttgcacta atccaaggac attggctgca catgtatttg taatagtcac
    28501 ttcttctccg tcttccatca ctaccttgac gtaatcagca gggtctcctt ctttaggaga
    28561 gaaaaattca accttaggac ctttgatttt ttctgtctcc gtaaatactg cataatctag
    28621 accggcaata tgtgcagaac gtggaccgac atcgatgatt cctgactgat tagcccgtac
    28681 catcgaaccg cctgccacac ctagaacccg aacatctaat gaagagatat atgttgggtg
    28741 gccacctaca attgaataat cgattgctgg acgtccattt ttaatgaccc cgatattggt
    28801 tgttgttccg cctacttcaa aataaacacc attagacgct ctcaagtaca tcaaagagcc
    28861 catgactgat gctgctggac cggaaagcat ggttaaaaca ggtcgttttt tcatttcatt
    28921 gatttccatt actccgccgt ctccgcgcat aatcatcaaa gaaacattga cccctgcttc
    28981 tcgtacggaa tcttctgtag aggttgccgt atctagcatt tttggtaaaa tagatgcatt
    29041 gattgctgct gtccgagttc ttcttgtcaa tccatataat tttgtgatgt cagaagccat
    29101 cgttgtaggg atcgatttta ccgatgctgc ttcataaaca actcgttctg gttccccatt
    29161 atctactcca aatgccatag aagaaaccaa gacctccgct ctttctcttt ctaaagaaga
    29221 aatcgtttct gaaacacggt ctacatttaa gtgtttgaca ggtaagaatg cattgacaat
    29281 ctctattttt ttcttattcc ctaaatcgat atcgttcaat ctagtttgtc gttttgctaa
    29341 aaaaccttct aatccacctt ttgccatccc gattacgccg acttttgcta cgtctccttc
    29401 aatcagtgca ttagtcgctt gtgtggtaga atgtgcaaca aacacaacat cttctggact
    29461 gatattattt tccagcaaac aattttgaaa agactgaacg acacctgcgg ctactccacg
    29521 gacatcatcg tgggttgttt tcactgaaga ttttccgatg atttcgtgcg tcgcattatc
    29581 gattgcgact gctttcgtat gtgtaccgcc aacatcaatt cccattcgca caaaacgttt
    29641 cattttcttt tcctccattc atctaatcgt ttttatcgac gttttgttta ctaggcgttc
    29701 ccgtacaata agaacgtgat caggcataag atcgctccga tgatccaacc agttgggatg
    29761 gatttcttca tatattctcg agaagttact tttgtataac ctaatcccca agcaacccaa
    29821 gattgtgtaa tatctaaatg ctgaggagca atcgttgtga ttgcaaataa cgggaacaaa
    29881 aaggctggcg acatcgttgg attaacagct aatacgacag ctaaaatagc tgagcctgaa
    29941 ccgactagat tcattggacc gcggaaaaat cctagtggtg tcaatatagc aaatacgatt
    30001 gccaagacta atggtgattg cggtatccaa tctccaaaaa tagctgaaaa ataaggagaa
    30061 gcatacgcag ctgaattatt gaacatcgct aaggtcatca agaaaccaac cattggcgca
    30121 acatctactg cgccatctgt aaataatttc gcaaactgac gacagatttc tttataacct
    30181 ccgcgcaatt ttccagtaaa cagcaatgcc cagatacctg ctagaataaa acctaagatg
    30241 attggaagat ctaaaagaac gacccctaaa actggtaata atacggctaa ccatgagatc
    30301 attggtgcgt cgccaccttc agctggtacg tttgctgcca ttgcatagcg tttctttttg
    30361 ttcattgata tattagcgac cgttagtacc acaactaagc ttacaatcat cccaatcata
    30421 ccaatttgga aatagtcatt ataagtatag ctttcagccg ctggattgaa accagcataa
    30481 atcgtttgat attgtttgaa attgacgata ttagcaaaga ttccagccat aatcgagccc
    30541 ataaatgaga acagtgcaac cggtactgaa atacctaatg acatcaagat tggcaacaca
    30601 ataactgcaa tagaaatcac tggtccgatt cctgtcatcg acatgaatat aacagcggtt
    30661 acaatacata gtaagctcat cgtgattctt ggcttgtccc ctccaagttc aactaccttt
    30721 cgaatcaggg tagctgcgat tccgctatct actagaacac gcccgaaaaa ggcaccaaag
    30781 aagacattta ccaagatcga tttggcgtac tcagctggcc ccgtcgtata gatatgcgtc
    30841 aacacatcaa ttactccttg cccttccatc gccgggtttg gtgaaaacgt gtttccgact
    30901 agcgctaatc ccatccatac agtagccatg atggcaaagc cgaccatcaa gttgtagcct
    30961 tttacacagt accagaccat gaaaaagaat gagataacta acaaaatacc aataataata
    31021 tcactcatgt tattcctccg tccattttgt ttttctgata agcaaactaa tatatcagtc
    31081 aaactcattc taacttatgt agttagcgct ttcattaaag ggtgaccata aatgttcagt
    31141 tttcaattaa aaaaccttaa ttatgctatt ttagacttat acgttattgt cctgtttcat
    31201 ttgatggagg attgtaaata aaaaaagagg aaagaacata acttttcgtt atcttctttc
    31261 ctctttataa ttgtttagct tatttagcca aagcttcttt tgcttggtca gccaatgcag
    31321 taaatgctgc tgcatcgtta acagccaagt cagctaacat tttacggttg atgtcgattt
    31381 cagcaacttt caaaccgtgc attaattttg agtagctcaa gccattcata cgagctgctg
    31441 cgttgatacg agcaatccac aatttacgga agtcgcgttt cttttgacga cgatcgcgat
    31501 atgcgtagtt gtatgaattc attacttgtt cttttgctgt tttaaataaa gtgtgttttg
    31561 aaccgtagta accttttgct aattttagca ctcttttacg acgtttacga gttactgttc
    31621 cacctttaac acgtgccatg tataattcct cctaataaaa ataaatcttc agattctgtt
    31681 atttgtactt cttatttcat ttttgctagt tgttggcgaa tacgtttgaa gtcgccacta
    31741 gaaaccatgc ttgctttacg caattgacgg cgttgttttt ttgttttgcc gtggaaacgg
    31801 tgacttgtaa acgcgcggaa gcgtttcaat ccgcctttac cagtacgttt gaaacgtttt
    31861 gctgatccgc ggtgtgtttt ttgttttggc atgactatat ttcctcctca aaatctttcg
    31921 ttatccgact atcggctgtc caacagccag ttagtcattc aaattactta ctgtcgtttt
    31981 tcggtgccag cgtcaagaac atgctgcgtc cgtccatttt cgctttttgt tccactgttg
    32041 caatatccgc agtttcttca gctaagcgat tcaagacttt ttgaccaatt tctttatggg
    32101 taatggcacg gcctttgaaa cggatagaag ctttcacttt atcgcccttt tccaagaact
    32161 tacgtgcatt acgaagtttt gtattgaagt cattcaagtc gatcgtagga cttaagcgaa
    32221 cttcttttac attgatcact ttttgttttt tacgcgcttc gcggtctttc ttttgttgtt
    32281 cgaaacggta tttaccgtag tccatgattc gcgcaactgg tggtttcgca ccaggggcaa
    32341 ctaacacaag atccaagttt gcttgttctg caatattcaa tgcttctgct tttgtcttaa
    32401 ctcctaattg ctcaccatct gaaccgatca atcgtaactc tcgtgcacga atgccgtcgt
    32461 taaccatcat atcctttgct atggtcattc acctccaaat attatgagag aaagaaaagc
    32521 ttaccgaata aacggcaata aaaaaacgag ttctcttaca gaacccgcgc gtacttcacc
    32581 taatagaacc agtgaaaact atctagccca gtgacattat caactaaggc gagaagcggg
    32641 tgcttctgct tttatttctc aaccatcata gtatagcaaa aactaatctt tttgtcaatg
    32701 tttttcatac agtttgtctt ccttttctga aaatgattag attagcagac tttctagcag
    32761 tgattcccgc ttctttgcta tactaaagaa aaacgaggtg aacccacatg aaaaaaatcg
    32821 aacctattcc accttctctt cctcttctta caaagggaga gttttattta gaagatgagg
    32881 agatcttctc tggtcttctg atcaacgggg gtgaccaaag ctatctcgat gccaaaaata
    32941 tggtattaag agagagtcat atccaaaaac ttacgatgca aaaaacagtt ttacgtcatt
    33001 ttgaatgcag taatgtcatt tttgaaaaat gtgatttttc caatctggag tggattggtg
    33061 gcagtttcca ccaagtcgtg tttcatcaat gtaaattaac aggtacgaat tttgccgaaa
    33121 gttatcttcg agactgtact ttcactgatt gtattgcttc gatggcttcc tttagtagta
    33181 caaacttgaa agctgtctcc ttcgatcatt gtcaattaga agacagcgaa ttttatgagg
    33241 tcacttggaa aaaccttttt ttgtctgaca atcagttgac cggttcgaac tggtttcgaa
    33301 cttctctgaa gggattagac tttacgacta atttcttcac agcaatcgct ttatctcaag
    33361 attctttggc gggactaatc gtcaaccaag agcaagcact tgtgatcgcc caagcccttg
    33421 gtttgcagat caaagactag atcaacgaat ccgccgccac agtgcaataa gcatccaaat
    33481 agataagacc aaactgatga tcaaggcgat tccccatccg aaaacggaat gggtaaaggg
    33541 taactggaga ttcattccga agaatcctgt cacaatagtt ggaacggtca agatcagcga
    33601 ccagaccgtc aaaaatttca tcgtgtcatt caagttgtta ttcagcaaat tattataagt
    33661 gccagagagc tggtctaaaa tctgcgaagc taagttcgtc atttcaactg cttgtcgtgc
    33721 ttcgatcagc gcatcgtcca actcttcttt ttccttttct gacaacttgc gataaatagc
    33781 caatgccttg atttgttcta aaagtactac attttgtttt gttcctgtaa ctaagtaaac
    33841 caaaccgatc tctaaatctg aaagttgcag cagattcttg ttcgttgttt tttctcttaa
    33901 tttcagatta agccgctgac gttcactatt cacttcctcg attaaaggaa aaaaggaatc
    33961 agaaatcaaa aacaatgtac gaaacaataa actgtataca ctcatgtctg gcattcgttc
    34021 caaaagcgat cgaattagtc gaatcacgta ctcggtattc ggcgtggtaa atgtaaacaa
    34081 accgtcttct tttaaaataa aagcgatagg actagtttca taatgattat cgactttttg
    34141 ctggtgcgga acatttgata caagcagtaa cgcttcatca aaagaatcgt actctacgcg
    34201 cgcccgttca tttttatcta gagaataagt cagcatctcg tcactaatgc catattcttc
    34261 ttttaagttt ttaacagcta gcacatcgtt ttgctgtaca ttgatccaaa aattacggtc
    34321 ttgattaaaa tagtgttttt cccgcatact ctatcctcct gacatttgtt tccagtataa
    34381 agaaaaaagt cgctcaaata aaggtttacc ctttatttga gcaactgtaa atcaaatggc
    34441 tactttggtt tggcgaatct ctgctaattt ttcttcgatc attttcaaga cttcttcttt
    34501 tgcttgagga tcttccacaa aattatagcg gtctccatcg atttggattt ttggactttc
    34561 tttgtattct tcataccatt gatcgtaacg tctgttcaat tctttgtaat aatcgtatag
    34621 acttggatca aaggacaatt gttcatacga acgaccacgt ttttcgatac gctccaacat
    34681 cgtatcaaaa gatacacgaa tatgaactaa taaatccgga tgttttttat gggcagcata
    34741 gggaagttct tccatcatgt tttccagcag ttcatcatac acacgaactt ctgtttctgt
    34801 tgctcttcct agatctgcat ttagatggaa aagcagtgaa tcttcataaa tcgaacggtc
    34861 caatacatta ttgtcatctt tcattgcttg ttttatgctt gcaaaacgtt tattcaaaaa
    34921 ataaatttgc agcaaaaaag catactgttc agggtttgca taaaataagg gcaagacttc
    34981 attatcatca atcgattcat aaaatgcctg actatcaaag tgttcagcca tcatttctgt
    35041 taaacttgat tttccggcac caattgtacc tgctaaaacg atcacgctca tttacctcca
    35101 gctcacttca tcactaaata ttgtgtttaa ctccaacctt tttaaatata cccccaatat
    35161 ctagtagtgt caattgtatt tttttctagt caaagaagaa aaaaagtcaa aaaattctta
    35221 agaaaaaagt gttttttctt tagaaaacgt attcttagtt ttacttctga gatggtttta
    35281 gtaaaataaa ggaggaatgt tttttgagac ccttagtgga caaaaaaatt cgtgaagcag
    35341 cgcaactcgt tttgggcgct ttattttttg cttaaaaatc acttttacgt gagaaggagt
    35401 tagattatgg caaactatgg ccaaggagaa tcaagcggaa agatcatatt gatgggcgag
    35461 cacgcggttg tttatggaga accagcgatt gcctttcctt tctatgcaac aaaagtcacc
    35521 gcattccttg aagagctgga tgcaatggac gatcaactgg tttcttccta ctattcagga
    35581 aatttagccg aagctcctca tgcattaaaa aatatcaaaa aattattcat tcacttaaaa
    35641 aaacagcatg acatccaaaa aaacttgcaa ctgaccattg aaagcacgat tcctgctgaa
    35701 cgtggaatgg gatcaagcgc tgcagtcgcc acagcagtca ctcgtgcttt ttatgattac
    35761 ttagcatttc ctttgtctcg tgaaatacta ttagaaaatg tccagctttc ggaaaaaatc
    35821 gcccacggta atcctagtgg aatcgatgca gccgctacta gcagcttgca gccgatttat
    35881 tttacaaaag ggcatccttt cgactacttt tctttgaaca tcgatgcttt tttgattgtc
    35941 gctgatacag gaatcaaagg acaaacaaga gaagccgtca aagatgtcgc tcacctcttt
    36001 gaaacccagc ctcatgaaac tggacaaatg atccaaaaat taggatactt gacgaagcaa
    36061 gcaaaacaag caatcattga aaattcacca gaaacgttgg cacagacaat ggatgaatca
    36121 caatcacttc tggaaaagct gacaatcagc aatgattttc ttaatctatt gatccaaaca
    36181 gcaaaagata ccggggcatt gggtgccaaa ttaactggcg gtggacgcgg tggctgtatg
    36241 attgcattag cacaaacaaa aacaaaagcc caagaaatca gtcaagcact tgaagatgcg
    36301 ggtgctgctg aaacttggat acaaggatta ggagtacata cctatgttta aaggcaaagc
    36361 acgcgcatat accaacattg ctctaatcaa atattggggt aagaaaaatg aagaactcat
    36421 ccttccaatg aacaacagcc tttcgttaac attggatgca ttttatacag agacagaagt
    36481 catcttttct gatagctata tggtagatga attttatcta gatggcacct tgcaagacga
    36541 aaaagcaaca aaaaaagtca gtcagtttct tgaccttttt cgtaaagaag ccggcctctc
    36601 actaaaagct tcagtaatca gtcagaattt cgttcctacc gcagctggat tagcctcctc
    36661 tgccagcggg ctagctgctt tagcaggcgc ttgcaatact gctcttaagc ttggattaga
    36721 tgatctctct ctttcaagat ttgctcgacg cgggtctggt tcagcttgcc gaagtatttt
    36781 cggtggtttc gtcgaatggg aaaaaggcca tgacgactta agttcttacg ctaagccagt
    36841 cccttccgat tctttcgaag acgatttagc aatggttttc gttttgatca acgaccagaa
    36901 aaaagaagtg tccagcagaa atgggatgcg tcggacagtc gaaacatcca gtttttatca
    36961 aggctggtta gattccgttg aaggggatct atatcaattg aaacaagcaa tcaaaacaaa
    37021 agatttccaa cttctcggag aaacgatgga aagaaacgga ctaaaaatgc atggaacgac
    37081 tttagctgcc cagcctccat tcacttactg gtctcctgac agcttaaaag cgatggatgc
    37141 ggtgcgacaa ttgagaaagc aaggcattcc atgctacttt acgatggatg ctggccctaa
    37201 tgtcaaagtt ttagtggaaa acagccattt atctgaggta caagaaacat ttaccaaatt
    37261 atttagcaaa gaacaagtaa ttactgcaca tgctggtcca ggaattgcga ttattgaata
    37321 aagggagatt tttatgattg aagtatctgc accaggcaaa ctctatattg ccggagaata
    37381 tgcagttgtt gaaacaggcc atccagcagt tatcgctgca gtcgatcaat tcgtgacagt
    37441 cactgtagaa tctgcacgaa aagtcggaag tatccaatct gcacaatata gtgggatgcc
    37501 tgtacgttgg acaagacgca acggagaatt agttttagat attcgggaaa atccttttca
    37561 ttatatcctt gctgctattc gcttgactga aaagtatgca caagaaaaaa acattctttt
    37621 atccttttat gatctgaaag taacaagtga gttagacagt tcaaatggcc ggaaatatgg
    37681 attgggttct agcggtgccg tgaccgttgc aacggtcaaa gccttgaatg ttttttacgc
    37741 gttgaattta tctcagttgg agattttcaa gattgcagca ctagccaatt tagcagttca
    37801 agataatggt tcctgcggcg acatcgctgc tagctgttat ggtggctgga tcgctttctc
    37861 aaccttcgac catccttggc tccaagaaca agaaactcag cattctatca gtgagttact
    37921 tgccctggat tggccaggtc tatccattga gccattgatt gctcctgaag atttacgttt
    37981 attgattggt tggacgggta gccctgcctc tacttctgat ttggtcgatc aagttcaccg
    38041 ttcgagagaa gataaaatgg tggcttatca gcttttctta aaaaatagta cagaatgtgt
    38101 caacgaaatg atcaaagggt ttaaagaaaa taatgtaacg ttgatccaac agatgattcg
    38161 aaaaaaccga caattactgc atgatttatc tgcaatcact ggggtcgtca tcgaaacgcc
    38221 tgctttgaac aaattgtgta atttagctga acagtatgaa ggagccgcaa aatcttctgg
    38281 tgcaggtggg ggcgattgcg gaatcgtaat tgttgaccag aaatctggca ttcttccttt
    38341 aatgagtgca tgggaaaaag cagaaatcac tccactgccg ttacatgtct atagcgatca
    38401 aagaaaggaa aaccgatgaa tcgaaaagac gaacatgttt cactagctaa agcctttcat
    38461 gataaacaaa aaaacgaatt tgacttcgta cggatcatcc ataacccttt gccgcaaata
    38521 gcagtttctg atgtcgatct aagtacacag gcagttgggt ttacactaag cagcccattt
    38581 tatatcaatg cgatgactgg cggaagcgaa aaaacaaaaa aaatcaacca agatcttgca
    38641 atcgtcgcaa gagaagcaga tttgatgatt gccactggtt cagtcagcgc tgccttgaaa
    38701 gtcccttctt tagccgatac ttatacgatt atgcgacaag aatatcctca cgggaaaatc
    38761 attgccaaca tcggtgcagg gacttctgtc gaaagagcca aagaagctat ccgattgttt
    38821 cacgcagatg ccttacagat tcatttgaat gcacctcaag agttggttat gcctgaggga
    38881 gaccgtgatt ttaccaattg gaaagtactt attcaagaaa ctcaaacagc cattgacgtc
    38941 cccctcatcg taaaagaagt cggttttggc atgacaagag aaacgctcaa tgatctagct
    39001 gctttaggag ttcatacagt tgatatcagc ggtcgaagcg gaacaagctt cacccagatc
    39061 gaaaacgccc gtcgctccaa gcgggaactg agttatttag ctgactgggg gcaatcgaca
    39121 gtctcttcat tgcttgaagc aaatgaagca gacacttcta tggaaatcct agcctccgga
    39181 ggtatccgca atgcctatga catatttaaa gccctttgtc tcggcgcaaa tgccgtgggt
    39241 acttcaggaa ccgtactgac gcatctgatg aatcacggcg tagaagaaac tattatactg
    39301 atgaagcaat ggcaagaaga gcttcgtctg ctttatacta tggtcggcgc aacgaatacc
    39361 gcggccctac accaacaatc tctgatcttt tccggaccag tgaaggattg gtgcgaagca
    39421 agagggatcg acttagtgaa gtatggaaga cgcacagaaa agagcgtggg acaaaagtaa
    39481 agaacacttt tgtctcacgc tctaaacacg tacaaacggc ggaagtaaaa gcaactcctt
    39541 cggaaataag ctgaaattca caaaaatttg aaaagcaatt ttcgtgaatt ccttcttatt
    39601 tcttggagtt aaatgcttct gtcccagcct cttttttcga ataaatgatg tttaatcagc
    39661 tgtccttcgg tattcgttca atccgctgat ttcacaacct ctccactaaa cagtgtacta
    39721 aagctctttt aaggttgttt tccgatatag gcaagtatgc ctccgtccac atacaagaca
    39781 tggccgttaa cgaaatcaga tgcgctagaa gccaagaaga cagatggtcc agccaaatcg
    39841 attggctcgc cccagcgtgc tgctggcgta cgtccaacga taaattgatc gaatggatga
    39901 cgttcaccgt tttcttgtgt ttcgcgtaat ggagcagttt gcggtgtagc aatataacct
    39961 ggtccaattc cattacattg gatattgtat tgaccgtatt cagaagcgat gtttttcgtc
    40021 agcattttta atccgccttt tgccgcagca taagcactga ccgtttcgcg tcctaattca
    40081 ctcatcatgg agcagatatt gatgatttta ccgccgcctt tttcgatcat atccggaata
    40141 actgcttttg aaacgatgaa tggggcattt aaatccacgt cgattacttg gcggaagtct
    40201 gccgcatcca tttcgatcat cggtgtccgt ttgatgatcc ctgcattgtt gactagaata
    40261 tcaactgaac ctacatcttc tttgatctgt tgaaccatcg cttgaaccgc ttcttcatcg
    40321 gtcacatcgc agacataacc ttttgcatcg atgcctgctt ctttataatg cgctatacct
    40381 tcatcaactg attcttgatt caagttatta aatacgattt ttgctcctgc tgtagctaaa
    40441 gagcgtgcaa tctcaaatcc aataccatag acagcaccgg tgactaatgc tacttttcca
    40501 tctaaacgaa acatatccat actaaattcc atcattttct ctcccttatg cttattttaa
    40561 ttgatccatt tgcaccatat ccatgtctgt ataggtgata ttttctccgc acattgccca
    40621 gatgaatgag taattgcttg tacctacacc tgaatggatc gaccagcttg gcgaaataat
    40681 ggcttgttca ttgtctacaa ctaaatgttt tgtttcatct ggtttgccca tcatgtggaa
    40741 cacacgtgta tcttcattgg cataatcaaa gtagacataa gcttccatac gacgttcatg
    40801 tgtatgacaa ggcatcgtat tccaagcgct tcctggttct aaaatcgtat aacccatttg
    40861 taattgacag ctttcgcata catttggatg gatgtattgg tagattttac gttcattcag
    40921 tgtcaggcct tcaccagtct ccattggttt tacttggtcg atactgattt ttacatttgg
    40981 atatttatga tgagctggaa cagaactaat atagaattta gctgggtttt cagcatcttg
    41041 agaagaaaat acaacatgtc gtgtttcttt tccgatatag tatccgtctt gttttttcat
    41101 ttcttctttt tgcccatcga tctcgatgaa tcctggtcca ccgatattga tcacacctag
    41161 ttcacgacgc tctaagaagt aatcaacacc tagttctttg tctaatgtga tttctaattc
    41221 tttttcagta ggtgtcactc caccaaagat cattcgatcg ttgtgcgtat aagtcaaact
    41281 gattttccct ggttcaaata ccttttctac taaaaattct tttctcaatt gttccgttga
    41341 ataatgacgg atatcttctg gactgtgtgt ataacgtgtt tccatttctt gcattttctt
    41401 ctactccttc tatcgaatga tttttccaga gccgcatgct aaaaattgtg cgacttcttc
    41461 ttgactaaat tggttgcagt ccccatggat cgtatgcttc aaggcagaag ctgccgtagc
    41521 aaaatcaatg atttcttgcg gagacatatt ggataaaatc ccatggagca cgccacctgc
    41581 aaaagcatct ccgccaccta ctcgatccac gattggggtg attctatgga cttgggattc
    41641 cacatattca tggttcatcc acaatgtccc cttcaattca ttttcacttg ctgaatgaac
    41701 ggtacgcttg gtcgaataaa acacctgaat attcgggaac ttttcctgca ttttttgata
    41761 ataataaatt agaggttgtt cttctgcttc tggcgctttc tcgataccta acaaataaag
    41821 agcatccatt tttccagctg agcagatatc tactaaaggt aaaagctgat ggattacttc
    41881 gctggcttct tgctgtgtcc ataattttcc acgatagttg atatcgaagc tgatcaggca
    41941 gccagctttt tttgcagctt ctatcacctg tttcgtcatt ttttgccatt ttttagacaa
    42001 agctggggtg attcctgaaa catgaaatat atctattcct tggaataact cctctagcga
    42061 ccattccaaa tgatccattt cagcaaaact tgatccggca cgatcataga tcactgaggc
    42121 ggcgcgttcg ccactaccag tttccatata ataagtacct agtcttgatc caccagataa
    42181 tacctgtttc gtatctactc cataacattg caagtgcttt ttgactgctt ttcccaatgc
    42241 gttatcaggt acttttgtag caaaaatcgc ttcatgtccg taattagcta aagaaattgc
    42301 tacgtttgct tcccctccgc cataatgggc agcgaatcgt tcacttgaat cgatacgggt
    42361 tccctgctct gtggacaatc gcaacatgat ttctccgagt gtcactactt ttcccataag
    42421 cagctacctc ttgatttctt tcatttttgc tgcatactgt tgcgcgactt ccgttacttt
    42481 gtcaaaatct cctgtagctg cgggtgctaa aaggttccca cctactccga ctgttacgac
    42541 accagcatcg aaccactctt tcatattgtc cagactcaca ccgccagttg gcatgatatt
    42601 gacttgaggc atcggcgcct tgaaggcaga aatgatactt ggtccataag cgcttccagg
    42661 aaatagtttg acgatatcta ccccgctttt cagcgctgtt ttgatctcgg taatagtcat
    42721 acatcctggt aagtaaggaa tttgatacag attgcacatc tctgccgttt cttgatcaaa
    42781 ggaagggcta acaatatact ctgcgccagc taagatagcc aagcgagctg tgacagcgtc
    42841 taatactgtg cctgcgccaa tcactatttc tggttggtct ttgtagaatg acaaaagttc
    42901 tttgattact tgatctgctt gagggactgt aaaagtcaac tcaataccag ttagaccacc
    42961 tttgatgatt gcgtggcaag cattcaaagc ttcttctttg ctttttccgc gtaccaccgc
    43021 aatcactccg gcgtttttca atcgagataa aatatcaatt tttttcatct tctccaccta
    43081 tttcctattt agaaatttta ttttataact tggaaatcta tacccatatt atacggcaga
    43141 aaagaaaaat tgcaaacgct tttttcatta tttttgaatt ctttgctcga tttcgctttt
    43201 tgtttgcttg agggcactga ttatttgttc ttttgtctca tctgttaaac gatatttcgg
    43261 catacttaca ctaaatgcac caatgatttg ttcgtttttc acgattgagg ctcctataca
    43321 aaaaatatca cgttcgattt cttcatcgtc aaatgcgatg tgggtttctt ttaccgtttt
    43381 gatttccttt ttcaaccgta atgcattggt gatcgtatat tctgtgtaag ggatcaacgg
    43441 atgcttttca agataatcag tgtactgctc ttcactgaat tctgctaata cggctttgcc
    43501 catcgcagaa ttgtataatg gtcgggtgat tccgattttt gaagacatgc ggatcgtttg
    43561 atgttttgga tctagtttgt tcacgtacaa gatctcattg ttgtccaata cccctaagtg
    43621 gatagtctca tcgatctttt gctgcagctg ttctaaaaac ggtaaagtca actctaccaa
    43681 atcgatttgt tcaatgtttt tattcgcgta gcgaataagc tttgcaccta aacgatagtt
    43741 tttctctgaa tttttattta cgtagccgat catcaacaaa gtatctagta ttttcaagac
    43801 tgtagaagag gtcattccca caccctttgc gatcgtctgc aaagagacat cttggttttc
    43861 cgctaaatag tctagtattt tagaggcttt gatcaataca gttccataag gctttttatt
    43921 ttccatataa tattctccta tttctttata agaaaaatta tagcggaaat accatcaatt
    43981 taaaagagat atccagatat aaactaccat ttctctttac aggcaattta atgttgaaga
    44041 tccgcttcgt ttttacatca aactttgata taaagcaatg atttcttcta ctgtcgtttc
    44101 tcttggattt cctggtgtgc atacatctcg caaagcatct tctgcaattg ctggaatatc
    44161 tgcttctttt acacctaatt cagaaatggt ttctgggatt ccaaccgctt tgctcaaccg
    44221 atcgatctca ccacaagccg cttctcttac atcttctaaa gacatttcag ctgctccttt
    44281 aatccctaga acaagagcga tctcacggta cttttctcca gtatattctt tgttatattt
    44341 catgacagtt ggcaaaagtg ctgcacacgc tacgccatga ggaatgttgt accatgcact
    44401 taacgggtga gccatcccgt gtaccagtcc taaaccgaca tttgagaagc ccattcccgc
    44461 aatatactgt cctaatgcca ttttttctcg cccattttga tcgccatcta cagaagcttc
    44521 caaagaatgg ccgatgattt cgattgcttt taaatgcagc atatcagtca tttcccaagc
    44581 gccttttgtg ataaaccctt caatagcatg cgtcatagca tccatacctg ttgatgcagc
    44641 caattttttc ggcattccca tcatcatatc gctgtcgatg aacgcgacga ttgggatatc
    44701 atgaggatca acacaaacaa attttcggtg atttacttca tctgtgatga cgtagttgat
    44761 cgttacttct gctgctgttc cagaagtcgt tggtatagct aatataggta aacatggatt
    44821 tttcgtatca gcaacacctt ctaaactaat gacgtctgaa aactctggat tcgtcaaaat
    44881 aatcccaatg gcttttgccg tatcgattgg cgaacctcca ccgattgcga tcaaacagtc
    44941 tgcctctgcc tctttaacaa aagccactcc atcttttaca ttttggatcg ttggattagg
    45001 tacgatcccg tcgtataaag cataagcgat tgctgcttta tctagaagtt ctgttacttt
    45061 tgttgctata ccgtgctcga tcaatccttt gtcagtaatg acagctgctt tgccgaatcc
    45121 tcgctgctga aattcaggga cgatcgactc aatcgctcct tttccaaaat acgatgtttc
    45181 atttaaaatc attcgattac tcattttcta ttcctcctaa tttgtattat ttttcacaat
    45241 aaaaacgaaa gaaccccaag caaccggttg cccagttgct tgttgctaca gttcaaagac
    45301 tggcatcaat tcccaactga ctggagaggc atctggattt gtctccatga tatcttccat
    45361 gtacttccac catttttgat tgatagatgt atttgccatt ttttcccata acttttcatc
    45421 ttcgatcact aagtaaccaa ataacaaact tgtactcgga tcaagataga tcgaatagtc
    45481 gatgaccccg tgttcacgaa gtgctgtacg catttctggc caaagttcat catgtctttt
    45541 tttgtattct tcttgttttt ctgggtaaac tttcatgcaa aaagcttttt tcaccatgtc
    45601 agacacctgc tttcgtatga acttcctcca aataacctgc ttttggagtg acaccaaacg
    45661 catctgctaa atgccacagg ttttcgtccg tgattgtttg caagattggt ccttgcgctt
    45721 tgacgtacgt atagacttct gctgcctttt ctgctgtctc gatcaaacca aagacttcat
    45781 ccatgtcttt tcctgacccg tagattccat gttgtggcca taaaaccaat cgagtttctt
    45841 tcattttttc agctgttgct tccccaatct catttgttcc cggaaccatc cacggaatga
    45901 tggaaattcc ttctggaaaa acaactaagc attccgtaca catttgccat agcgtacgac
    45961 taaaggaacg ttcgtctaat tcatgcgtaa aactcatcgc taatagatga ctagcatggc
    46021 aatgcataac gatccgatta tctgggtcta cttctaaacg ggcgatatga ctcatgaaat
    46081 gacttggcaa ctcgcttgta ggaaggcatt cgttttccag cccccacaaa agttccagtg
    46141 ttttgccatc ttcagcaact cgaacgattc caagattctc ggcaggttcg ctcgctacat
    46201 ttttaaaata tcttcctgaa ccagttacaa taaaatagca tcctgctaaa cggctggcat
    46261 caaaaatcat cgagatgcgg cggatcactt tttcaggatc caagaacggt gtgatctctt
    46321 cttcttttag taaataagaa atattcccgc catttctttc atcccatccc aagcgataaa
    46381 gattcgacgt cgtttcgatc atttctttca cataaggcgc ttgtaaaata tctatttttt
    46441 tcaatatagc cacactccta ttttcttaat tccaacacgt ctttttgata ttcctggact
    46501 tcttccagcc aatccaaacc aactggaact tgatttttgt aacagaaata attccataca
    46561 tcggcatacg ggaaatcttt caattcttcc gtataagcca ttcttgaagt aaagtcaaaa
    46621 gccagctcca tttcttccaa tcgctcaact ggttccagca tagctttcag cagtgctttt
    46681 tgtgtatttc tcgtaccgat gacccaagca gcgatacggt tgatcgttgc atcaaagaaa
    46741 tccagaccga tatttgtttt ttccaacaat tcgttacgga ctaattcttt agcgatttcc
    46801 tgcaattcat catccatcaa aaccacatgg tctgaatccc aacgcactgg acgggaaaca
    46861 tgcaacatga ttcctttgct gaataaagct aatgaagata atttattgga aatcacttct
    46921 gttggatgaa aatgtccagc atctagacaa acaagtttgt cacgagtcaa cccgtaaccc
    46981 atataaaatt catgggaacc gactgtataa gcttcagcgc ctaaaccaaa tagtttgctt
    47041 tctactgcat cttgtacata ccgttcatct aactcttctg taaaaatttc atcgagtgat
    47101 tccattaaac gttcccgtgg ggtaaaacga tcaatcggat tatctttcat cccatcaggt
    47161 acccaaaagt tattgatact tgtttgccca gtttctttcc caaagtactc cgcgattttg
    47221 cggcttcttt tcccgtgttc gatccagtat tcacgaactt ctttatctgg atgagccaat
    47281 gtaaagccgt ctttcatcat tggatgcgaa aagaaagtcg gattgaagtc aagaccgatt
    47341 tcttcttttt ttgcccactc tacccatttt tcaaaatgtt tcggctcaat ttcatttaga
    47401 tcgacagatt cttctgtatc caaatagata gcatgaaggt tcaatttgtg tttgcccggt
    47461 atcaacgaat aagccttctc cagatcttgt ctaagctctt caggtgttcg tgcggctcct
    47521 ggaaaattcc ctgtcgccat gattccaccg gtcaattcgc catccggtgt caagaaacct
    47581 ttgacatcat ctccttgcca gcaatgcata gaaattttga tgtcctgtat cttttctagt
    47641 acagcatctg tatctacacc gatagttgca tatttttctt ttgcttgttc gtaatttctt
    47701 tcaatcgtgt tcatacgatt actcctttga ttttattcac gtaccattcc atcgtgtttt
    47761 gcttattttc atctgggtaa aaagtttcac atggaaacga ctgtttgatt acatttcttg
    47821 cttgttctat attcgaaaac tgacctgtac tgagcatttg catggcaaca ttcccgattg
    47881 ctgtggcttc actaggtcct gcttctacaa tgattccggc ataatctgct gtcaattgat
    47941 tcagtagacg attattcgat ccgcccccta cgatgtgcag cttttccaat ttcgttccag
    48001 ttaagtgttc tactttgatc agctcttgtg cgtaacaaag agcaagatta tcatacacac
    48061 aacgagcgat agctcccgat gagacaggga ctggctgctc tgtttcaaaa cagtattgct
    48121 gaatctcttc tatcatattt gggggattca aaaatcgagg gtcattgatt tccatcactt
    48181 gctgcagcgg tttttctttt gcagcaagct ctgccaattc cgcataactg tactcgtact
    48241 cttgcattcg ggcaacttcc tggatcaacc acatccccat gatgtttttc aaaaaacgca
    48301 ccgtgttatg ggcaccccat tcattcgtat aattttcaga aaaagcattg cgagaaacat
    48361 tggcgatctt tgtttcaatc cctaaaagcg accatgtccc actgctgata taaccccaac
    48421 gttctccttg acctggcgtt cctaatacag ctgacgccgt atcatgactt gctactgtga
    48481 taaaggtcac atctggcaaa tcatattttg aaaattgttc tttttgcaga tctcctagta
    48541 tcgttcccgg ctctgtcaat ggtggaaata attccttcga gacaccgatt ttttcaagaa
    48601 gttctatttc ccattctttt gtccctgcat tcagcagctg catagtagat gcattagttt
    48661 tctccgtcac catgttacca gtaaagacat accctaaata atcaggtatc agtaacaatt
    48721 tatgtgcttt tgccaaattt tctttttctt cagcaaacaa ttggaacaag gtattgaaag
    48781 gctggatctg aatccctgtc tgctcataca acttatctag agaatagatt tcagaaaact
    48841 gttctactgc tccattggta cgcttgtccc gataagcaat cggatcattt atccgatccc
    48901 cttgttcatc aatcagacag taatcgactg cccatgtatc gatccccgca aaaaggcgat
    48961 caatccctgt ctgctttaat ttttcaaatc caatcaaaat ctctttgata agataatcga
    49021 tcttccagcg atcgtgaccg tctttgttcc gaaagccgtt tttgaaccgg tgtacttctt
    49081 ccaagatcaa agaacctttt gcatccctcg tacttttcat tagtcgtccg cttgatgcac
    49141 cgatatcgat ggcaacaaat gttggattca tttctccacc tcttttactt tattgttgat
    49201 tagcacttta ttaataaagc gttatcattt attgacaatg ctatcataat acagaatctt
    49261 atattttgct tcacactatt ttggtatata attgcattat cttgacaagt tgggagtgtt
    49321 tacatggatt actgggaaaa agccaaccac tcatggtcgg aagactcaaa aagagtgatc
    49381 ttgacgccta cacaaagaag caggaattta tttttttata tccaagaaat cggttacttc
    49441 aaagcaccaa tcccttacta tactgaacga gcacatttac cttcttactt gataaagttt
    49501 actttgtctg gcaaaggttc gcttacatat aatgataatc agcaacagct aaaagctgga
    49561 gacttatttt ttattgactg ccaagaatat cagcgttatc aaacgatcag cgaagaacca
    49621 tgggaaatgg attggatcca tttgaatggt cagaacgtcc aatcatttta tcaggagttc
    49681 acaaaagacg gccataacac cttccattca acaggagatc catcagaaaa caagatccac
    49741 catttgatca cagaattgat ccacatgcaa caaggcacga atgcccgtac cgatttccag
    49801 tcctctgtag ctatccacga gttgctgaat gaactgattt tgcaaaagta tcagctagat
    49861 ttcgaagaag aggatattcc cgcctacgtt tacaaactga agcaatactt agacgaacat
    49921 ttcaaagaaa cgattacact tgatcaatta gaacaacaat tcttattgaa taaatatcag
    49981 ctgagcaaag atttttccaa atttatcggt tctccaccaa tcgattattt gatcagcaaa
    50041 aaactttctt acgctaaaga ccttcttcgg tatacagaat ttacggttcg aatgatttcc
    50101 gaagaaatag ggattgagaa ttttgcttac tttagccgcc tgttcaaagc aaaaacagga
    50161 ctatcgccga gagactttcg taaattcagc tgattcatat aatatgtacg tcattttttc
    50221 aaaatatttt gaaatatata gataaaaata aaataaccgc agtttctgct tctatgtaaa
    50281 caacagcagc tactgcggtt attttttagt gatcattctc attgttcatc ttcaaaatgt
    50341 tcataatgaa ccaactgttt ctgtgtatca cgtttcaaaa attctttagg cgttatgccg
    50401 aactctctct tgaatgcttt cagaaaatgc ggataagaag aaaaaccact tttactatat
    50461 acagcactag agctgcggta ttctcgcaac aagtgcttgg catagatcag ctttttcttc
    50521 attacatact gatgaaacgt aaatccagtg tactttttaa attctcgtgt gatatggtat
    50581 ttgctgacaa aaaactgctt ttccatttcc tctaaagaaa gcggatgaga taaatttttt
    50641 gagatatatt gtaacatttg ttccacacgc tcattaggaa ctacagcatt ctgttcttgc
    50701 gcactctctt ctttttgtac aacttgattg agaaaaatca agtattctac cagagcttgt
    50761 tcatatttta tgtcttcacc gtaaattttt ttatcaggag gatggtcgat aaatgataat
    50821 ttttgcttca atacttcctg ttctatttgc aatacacgac tcctcttctc tcccattggt
    50881 tgaaaacaag ccgatagatt agaccatggc gtggaacgac tttctagaaa agaaggggtg
    50941 acaaagatat agacacgctc gaatacatct gaagattggc ggacgatacg atgcaaatca
    51001 ttggaatgga tgagcagcac acttcctgct ttgatcgtaa attgtcgtcc gttcaaataa
    51061 aaagtcgctt ctccctttaa tgtagcatgg atctcataga aatcatgatg atgatagatc
    51121 gttttatttt ggctgacgac atctttgaca tgatagattt catactgatc agaaaccatc
    51181 gtattgactt gttcaccgct gattttcttt tggagcatgt gccgcacctc cttttgttca
    51241 cttatgcaca attttattat accacgtttt ttgtaatcaa aaagagctgc ttttatatac
    51301 tcttataaaa aagaagagac gtggcatctt ctagccccgc cccctccatt ctaaatatta
    51361 gttaaccaac gtcttcttta cattattcaa gaagacttct tccataacga atcccatcat
    51421 cttttgtttc ttcaagaacc acatacaggt acggacgatt cttcatgatg atttctttga
    51481 tttttgtgta ctcgatcaca ccgtctccca aatttaccgg cacgattttt tcctgttcga
    51541 cacgaaaatc tttcaaatga accgcacata tcttttcacc aaaacgttca aaagcttctt
    51601 ctaccaattg gacttgttct tgataatcac tacttgtgat caaattggtc ggatctaaaa
    51661 tgattcctag atagtccgaa tctactgctt ggatcagctg ctctaccctt gctaaagaat
    51721 acaccggatg attcaaacct ggttctatcc ccaccatcat ttggtatttt tctccagctt
    51781 ttaccaagcg acggataacg gacacagctt cagcaaatgc ttcatctgtg aaattttctt
    51841 ccgtatattg gatttccggc aaaacacatc cagtttccga agcaaccatc gacgcaccaa
    51901 aggaagaagc atagcgaaga tactgctcga actttgtcag caactcttgt cgtatcgtta
    51961 aatctggatg aatcatattg atatagcaac ttaaaattcc tacggtcact tcttgctttt
    52021 ctaaaactcg tttgacataa tttcccatcc cagaattgat ctcatttgcg ccagaaggta
    52081 tttcaggaaa cgaaagacct aaggcgagct gcagtgtatg aatcccttgt tcttttgttt
    52141 tttctgcaag atcttctatc gtttggactt cggaaagatc atgtcctcta attgtaatgt
    52201 tcaacataac tattctccta actcagcata ttgggtagcc acatgacgat ttgcgggaag
    52261 taagtaatca aaaacaagac gactaagata accccataaa aaggaagaag cggtttcagc
    52321 attgcctctg cttttacgcc tcctacactt gcaccgacat atagtcctgt acctactggc
    52381 ggcgtgatgg agcctacaca cagattcatg attgaaaaca acccgtaatg tacaggatca
    52441 acccctacac ttgtgacgat tggtaaaaat attggtgtaa aaatcaagat agcaggcgcc
    52501 acatccataa acatacccac cagtaataac acaagattga ctaataacag aataataaat
    52561 ttattatccg ttacgcccat aatcaacgaa ctaattgcct gaggaatacc ggtaaacgcc
    52621 atggcgaaag acatcatgga agaagtagcc aataagaaca tgatcgtccc tgacattgct
    52681 actgattgaa acaacatatt aggtatatct tttagtttta ccgttttata gtaaaacata
    52741 gcaatcaaca gggagtaaat gacagctatc gctgaagctt ctactgctgt aaatatcccc
    52801 gtcaagatac caccgataat gatgacaatc aatagaaggc taggaatcgc ctcttttatt
    52861 atttttccag catgagccat ctgtcctttt tcgacaacag ggtatttatt ctttttagca
    52921 acgatgaatg ctactagcat gattcctagc gcccacaaag cgcctacgag ataacctccc
    52981 ataaacaaag cagagatcga agcaccacca gcaacaagtg aatacatgat aaaagcagta
    53041 cttggaggaa tgaccatacc tgtcggagct gacgcgatat tgaccgctgt cgcaaacttc
    53101 cggtcatatc cttcttccac ttgctgaggg atcaacactc cgccaatcgc ggtagaagcg
    53161 gcaatggccg agctagagat ggaaccaaat aaagcattcc ctaacacatt cgtatgagcc
    53221 aaagcccccg gcacgcgccc gacgaacaac atcgcaaaat ccactagttt cttagctatc
    53281 cctccattat tcatgataat gcccgagaga acgaaaaacg gaatagcgat cagcgaaaaa
    53341 ctattgatac ttcccaccat tttttgagct gaactaaaag cagaaacatc aaacggtata
    53401 actaacaata aagtcatcat cgatgagaat gcaatactta tagcaatcgg catccctaat
    53461 acaagcaaca caacaaatac aaaaaataac actaatccgg cttctattgc cattttattc
    53521 ctccattttt atattttttc ttgaattgct ggcttgttgc ttaatgtatc gattagattg
    53581 atcaggctat agataatgat gagtaccccc gatacaggta ctgacagata aacatatccc
    53641 ataggaatac ttaaagaagg cgagatctgc cccattgtca atgtgacagc attccatccg
    53701 ccatacacca tgatcatcgc agcaaatacc agaaataaac tttgaacaac aacttctaaa
    53761 attttacttt ttttgccagt taatttttca cttaaaaccg ttacggctaa atgtgctttt
    53821 tgtcccacaa catacgcgga agacaacatg gcaaaccaga caagaccgaa tcgaacaagc
    53881 tcttccgtga ccgtatttgg atttcctaaa accgttctgg aaaatacttg ccataaaaca
    53941 acagcgacca tcccaacaag caaaacagct cccaggatct ctaatacctt gtttaaaatc
    54001 attttcatca cagctacgct ccttgctctt ggatcttttg ataaaggtct ttgtaggtag
    54061 gatcgttttt gaatatatca tgaagtgggg caactgcctc tcgaaacggt gtattatcta
    54121 catcattgaa ttggactcca tgttcttctt gtgctaaagc tttttcttct tcaattgctt
    54181 ttgcaaaagc ttcaatttca aaaaccgttg attctttcgc tgcttcttca atggcttttt
    54241 cctgctcatc agttagctgt tctttgatgg catcattcat gatgatgata tctggcacac
    54301 gtgtatgctc atcataggaa tagtatttag ccacaccacc atgtccagct gtatacaaga
    54361 caaattcatt gttttctgat ccgttgatca aattggattg aagagacgtg tacacttcat
    54421 cacttcccat aggtacgggc gaaccgccta aaagctctac cattttgata gcggtttcgc
    54481 tttgcatcac acggatcttc tttcccttca aatcttccgg cgtatggatc ggaccgtcta
    54541 ccatataaaa gctgcgctga ccagaatcat aataggtcaa tccggtaaat cctaaatttt
    54601 tggtcgattg gtagattggc tccattattt cttcattatc catcacctta aagaaatgtt
    54661 cttcatcatt gaatagataa ggaactgaaa aaatcgaata atctttagag aaactttcca
    54721 acgctgaggc acttactttt gtgaaatcta ctgctcctga ctgtgtcaat tcgatcagtt
    54781 cacgttctcc acccagttgt ccatctggga aatactggat ctgtacggaa ccatctgttt
    54841 tttcttttac taaacgccca aattcattca ttgcatcaat tactggctgg ctgttgcttg
    54901 cataagcaaa gcgcaaggta actatctcat ttacatctgc atctgcgaca gttgccacac
    54961 tagaattatt tccgcagccg cttagaccta gcagacctag gctaagcgaa cttgctgcaa
    55021 acagcagtga tgtttttttc atatgggttt cctttctttt aacaataaga gacgagcaat
    55081 tctgtcaaac aaagcaccgt taacgactgt ccataaggca ttgctgtgat tccgatattc
    55141 cgataaaaat ctaaactgtc ccccattcct gtcccgattg aaacgttttg aacttctcct
    55201 ttttcatcga tttgttcaag caatccttgg atagcacgat aagcgacttc tttatattct
    55261 tgtggtaaat aacgtttatg gaccgctttc aaaataccgt aagcaaatcc tgcagttgca
    55321 gaagattcta cgtaagaaga aggatcatcc aacaacgtat gccataaacc gctttcagac
    55381 tgataagttt ttaatgcacg cacttgtgct tcaagtgttt cgatcaataa tttgcgcaat
    55441 gcatcttctt tcgataattc taagatttca atgatctctg gaatagcaat cgtcaaccag
    55501 cagtttccac gagcccaaaa tgcttcggca tagttatgat ttccttcaaa cgtccagccg
    55561 tgataccaca atcctgttcg tttatccatc aagtatttga tatggatcat aaactggtaa
    55621 cgagcttctt ctaaatattc tgggcgattc aacagtttcc cgatttttgc caaaggaagc
    55681 actgtcatca tcaatgtatc gtcccacaac tgattcttat tttccggtcc atatgtggca
    55741 tgctgtaacc cgtcttcatt tgtccgaggc atgtcattca tgacccattc tgcccattgt
    55801 tctaaataag gcaaatagcg tgaatctttc gtgtcttcat acaagtaagc catagtcaat
    55861 agcggtgcca ttgtattgac gtttttgggc ggagcgcctt cttccatacg atcctcaaac
    55921 cattcattga ccacttgata agcgcgagga ttttttgtca tctggtaatt tttataaata
    55981 ccatatagac cgacaccttg tggccaattc cacactgtcc agcttttgtc atctactttc
    56041 agtccatcaa agtccaacaa aaattcacca ttatcgtctt tgatcgttgt caggttttca
    56101 acgagcaggt caattttttc tttcactaat tctttaggta caaaaatctc ttctttaata
    56161 atcatcttta cgccaccttt ttaaacgctt tcattttata tccttaatat agcagtaata
    56221 ccgtccacta taaatatcgc tttttgaaca aaaactgtct aaaattgacc taaatgttaa
    56281 ataacaaaag taactttcag ttacttggaa attaaaaaaa gaacttggag aaacaacgtt
    56341 ttccaagttc tttttcatct attttcttat cttttttcta cgctttcttt tgcttatccg
    56401 ttgtaagcat caatgatgat ctgtttcaat tcagagatca atggttcttt cggattagct
    56461 gtcgtacatt ggtcttcgta agctaattca gccatacggt ctacggttgt atctagtgtt
    56521 tcttgtgtga ctccttgagc ttttagactc atgttgatgc cgacagattt tcctaattca
    56581 taaacagccg ttgctaaagc ttcgactaat tcctctgttg tttcgccttt cagacctaag
    56641 aattttgcga tatctgcgta atctgtatct gcacggaaat agtcatattt agggaacatc
    56701 gcatgttttt gtggatcttt tgcattatag cggataatat gcggtaaaag aatcgcattt
    56761 gtccgtccat gagggatccc gtattccccg ccgattttat gtgcgactga gtgacaaata
    56821 cctaggaatg cgttagcaaa cgccatacca gccattgtag aagcgttgtg cattttttca
    56881 cgtgattcca tatcaggtgt ttttactgat ttttctaaat agtcaaagac taacttgatt
    56941 gcttgtaggc tcaatccacg tgtataatct gaagccatga ctgaaacata ggattcgatt
    57001 gcatgtgtta acacatccat cccggtatct gctgtgacag atgctggaac tgacatgacg
    57061 aattgaggat cgacgattgc gacatctggc gtcaatgcat aatctgctaa cggatatttc
    57121 acatgtgttt cactgtcagt gatgaccgca aatggcgtta cttcagaacc agtccctgat
    57181 gttgtcggga tacatacgaa ttgtgttttt tctggtttgt cgatcttata tgtccgttta
    57241 cggatatcca agaatttttg ttttgcacca aagaaagatg tgtctgggtg ttcatagaac
    57301 atccacattc ccttagctgc gtccattgca gaaccgcctc caagagcgat tactgtatct
    57361 ggcttgaagt ctgccatcag tttcgttcct gcgtatacgg tatttgtcga tgggttcggt
    57421 tcaacatctg agaagacttc gatcttcaca tcatttttac gtcgtgccaa tacttcacga
    57481 actgtatcgc agtaaccgaa ttggaccata ccagggtcac aaacgatcat gacacgttca
    57541 acgttttcca ttttctctaa gtacaataga gagttctttt caaagaagat ttttggcggt
    57601 aatttgaacc attgcatgtt atttctccgt ttcgctacag ttttgacatt gattaagttt
    57661 acagcagaga cgttacgaga aacagagttc ttaccataag aaccgcatcc caaagtcaat
    57721 gacggaatca tttcgttata gatatcaccg atgccgcctt ctgcagatgg tgtattcact
    57781 aaaatacgac aagctttcat tcgaagaccg aatttgacat gcaactcttc gttttctgaa
    57841 tggataactg ctgtatgtcc taatcctcca agattcaaca taccttcaca caaatcaaac
    57901 gcatggtccg tgttgttagc tttgatcatg gcaagaactg gagaaagttt ttctcttgat
    57961 agcggataat ctgggcctac gccttccaat tcagcaatca gcattttcgt tccttttggt
    58021 acactgattc ctgccaattt tgcgatttcc atcgcagaat gtccaacgat tgatggattg
    58081 accgcatatt ttccttcgtt catcactgca tcttctaatt tttgcaattc atctggtttt
    58141 acgatataaa cttggtgtgc ttggaattct gcttttactg ctgcatagat ttctttatcc
    58201 acgatcacag cttgttcaga agcacaaatc attccattat caaatgtttt cgaaacgatc
    58261 aaatcgttca ctgcacgttt aatttttgct gttttttcaa tataagctgg tacgttacct
    58321 ggacctacac ctaaagctgg ttttcctgta gagtaagcag acttgaccat tccagctcca
    58381 cctgtcgcta agacgatcgc gatacctgga tggttcatta gcatagaagt cgcttcgatc
    58441 gaaggatgtt cgatccattg gatacaattt tcaggagctc cggcagcgat agcagcatca
    58501 cgtaccactc gtgctgcttc tgcagaagat ttttgggcac ttggatggaa agcaaaaacg
    58561 attggatttc ttgttttcaa cgcgatcatt gatttgaaaa tcgttgtgga agtcggattc
    58621 gttgttggag ttactccgca aaccacaccg actggttcag caatttcaat caaccctttt
    58681 tggacatctt cattgatcac accaactgtt ttatcatgtt tgatattatg ccaaatatat
    58741 tcggacgcat acatattttt gattgcttta tcttcgtaaa caccgcggcc tgtttcctct
    58801 acagccatct tcgctaatgg catatgttga tccaaagctg ccattgccat ttcatgcacg
    58861 atatggtcta ctttttcttg atcaaagctt tccatttctt tcaatgctgc atttgctttt
    58921 tctgccaaat catcaatcat tgcttgtacg tcaattgtct tcgttttttc tttttctatt
    58981 actttagcca tcattatcct cctaatgggt catggttatt tgttaaataa ttcacatgta
    59041 tatcatacaa tatttatttt tctatgtaaa cacttttttg tgattttttt cacagttatt
    59101 tttaaatata aaattgcttt atttacaggt gtttgtatag aaaaatatat aaagttattt
    59161 attattattt atatgacaca cccgattatt caatattgag caatatatcc catgctctta
    59221 tctctttctt ttttgaataa aaaatgaggt aggacatctt tctgccctac ctcgtcttta
    59281 aataagcttt gatacttatt tttcttcctt ctcttctgtc tcttccagcg tttctgttgt
    59341 ttcagttttt acttcaactg tctcagctgg ttcttctgtt gtttcaacag tagtcgttgc
    59401 atcatttgtt acgacagtcc ctggtttgac cgttttgatt gctgcacgat cgtactcaag
    59461 gaagatccct tcgcagtcta aaacaactgt ccgtttgtca ttgtcgattt ctgaaacaac
    59521 tccatgtaat ccgccgatcg ttacgactga atcacctggt ttcatgctat ctaacaagtt
    59581 ttgacgttct tgctgttgtt tcttttgcga acgtgacata aaaaaccaca ttgctaatag
    59641 cgcgataaac atcacaagca ttggtaatgt tcccattttt ttcacctcac taattctgta
    59701 tctacaaaaa acactttagc agaaaaatgc tgataacact agttttttac ttaattttta
    59761 aaagattttc ctttagaagc tttttgcgtt ttctttattg aacccgtatt cttcaaaaaa
    59821 ggcttctcta aattcaagca aattatcatc catgattgct tgacgtactt gtttcattag
    59881 attcaacaag aagtataaat tgtggtaaga agtcaaacga atcccaaatg tttcatcaca
    59941 tttgatcaaa tggcggatat aggcacgtgt atagttttta catgtataac aatcacattt
    60001 ctcatcaatt ggacggaaat catgggcata tttcgcattt ttcactacta gacggccttg
    60061 agaagtcata catgtgccat ttcgagcgat tcgagtaggc aagacacagt caaacatgtc
    60121 tactccacgg ataacaccat cgatcaatga atcagcagca ccgacaccca tcaagtaacg
    60181 tggtttgttt tcggggatca acggtgtagt gaattctaat acacgattca tttctgattt
    60241 aggttccccg acagataacc cgccgattga gtaaccaggg aaatccatcc cgactagatc
    60301 tttcgcgctt tgacgacgaa gatcttcaaa ccctgctcct tggatgattc caaataatcc
    60361 ttgacggtct ggattagcat gagctttcaa tcctcgttct gcccatcgag acgtacgttc
    60421 tactgatttt ttcacgtagt cgtaactttc atcgaatggt ggacattcgt caaaactcat
    60481 catgatatca gagcccaatt tattttggat gttgattgct ttttctggtg ataaaaacat
    60541 acgcgaaccg ttcaagtgat ttttgaaatg cacgccttct tcttcgatat tccgcatatc
    60601 acttaatgaa aaaacttgaa agccgcctga atctgttaaa atcggttgat cccagttcat
    60661 aaatttatgc agtccgccag cttcttctac taagtcttca ccagggcgta gccataaatg
    60721 atatgtatta cttaaaataa cgccagcacc catttctttc aactcttctg gggacattgt
    60781 ttttactgta gctaatgttc ctactggcat gaacattggt gtggggaaag ttccgtgtgg
    60841 cgtgatcaat tcccctaaac gcgcgccagt atgtttttct ttcttgatta aacgatagcg
    60901 aatggcaggt tgcgtcattc atttccctac tttctatata aaatatcttg acaactccca
    60961 ccatcataat cgtttcaagt gctttttgca agttccctcg gcttccttct actttattta
    61021 atattctttt ttttatcctt tttttatgaa taacaaaaaa aagaaaaaca ttatgacaga
    61081 aatatttttt gatacatggt atattggtgt tattaaagaa aaggagggaa aaatcatgca
    61141 atcttctgca gaattaaaac gccaagcaaa agagagtcta aaaggacgtt ggggacaagc
    61201 tgttttacta aatctaatcc ctacattgat tggtattgct actattcttc ttattatgat
    61261 tcctattatc ttgtttgcca ccaccatata tgatggatca gcgagcgtct cagaagcgac
    61321 aacttcagga ggcggtagtt caggaggcgg tggcggaatt atttccagca tcattagcgc
    61381 tttgttcatg agcggtattt cttggactta tcttgatatt ttacgtggac aaaaagatca
    61441 gatcgaacca ttcaaagatg catttcgtgg attcaaaggt ttgtttatcg gcggagtgat
    61501 cagcttagct gtgctgatct cgatctttac aacattatgg ggtttgctat tagttattcc
    61561 tggaatcatt aaaagctatt cttattctca aagctatttt atttactacg atatcgtcca
    61621 agaaacagga caaacaccta aagtactgga tacgatcact gcaagtcgaa aattgatgga
    61681 tggttataaa ggacaacttt tctggttaga tctttcattt atcggctggc atatcttagc
    61741 attagcaact gctggtattg gttatctatg gctcagccca tacatttctg caacaaaagc
    61801 agcattttat gaacaattgc caaaagatat ttaaaagaga gtgtgacttg tgaccgaggg
    61861 ggtggcagga gaacaccgtt tatcgagagg cagtaaaaga aacattcagc ccattagcaa
    61921 aaaggaggtg tgacaacatt catgtcacac ctcctttttg tatagttcca gtaaaaagaa
    61981 aatcaactta gaaacagtta atcattcctc ttcttctgct tccggtaatc gatggaactt
    62041 gatcagttca ttcaccgcga ttggtacgat agataaaatc cctaccaaga accagtgatt
    62101 caaactgatg tgtactaggc cgaatagttc ttgcgtaaat ggcagcaaag caacagccgt
    62161 tgtaatagct aatgccagta acgccatttc taccaaagct ttattggtcg ctaaattgac
    62221 tttgaagatc gattggctgc tgcgtacatt gaacacatgc agaatcgatg aataagctaa
    62281 gatcaagaat gcaacagttt gaccgacatg ctgacttgcg tctacccatg ggctgatagt
    62341 atcgacataa ctacctaaat aataaccaaa caaggttacg atggtgaaga cccctgcatt
    62401 tatggcaatt ttttgcaata aacctcgagc aaagatccct tcatttttcg gaatgggtgg
    62461 ctgatccatg atgccgtgtt ccgctggctc tcttcctaaa gcaaatcctg gaagtccatc
    62521 agcaactaca ttgacaaaca gtagttgcac agctgtaaat ggtgctcccc atcctaacag
    62581 catcgcaatc aaaacaataa agatctccga aatattacag cttagcaaaa agttgatagc
    62641 tttacgaata ttctgataaa ccgaacgtcc ttgactgaca gcgtctacga tcgtcgcaaa
    62701 gttgtcatcc gtcaaaatca tatctgcggc actttgcgcc acatctgttc ctgtgatccc
    62761 catggcacac ccaacgttac ttgctttcaa cgctggtgca tcattcacac catccccagt
    62821 catcgctacg actgcacccg tacgttgcca agatttgacg atccggatct tgtcttctgg
    62881 cgttacccga gcatagacag agagagcttt gacacgcgca tctaattctt cgtctgataa
    62941 ttgatgcagt tctgatcctg aaagtgcttc tgacggatca ttcaagatac ctaattcttt
    63001 cgctatggca ctggcagtca ccacatgatc tccagtgatc atgactgttt tgatgccagc
    63061 ctttttcgct cgcttgattg ctcctttact ttctggacgt ggtggatcga tcattccgat
    63121 cagaccaatc aaacgtaagt ctttttccag tgcttcagaa gaaatatctt gcggttcttc
    63181 gtcataagta gcataaccaa cagcaatcac tctcagtgcc cgtttaccaa agcgatcatt
    63241 aacgatcgcc gcttgttcta catcgcctga actaaagcgc ggagcaagaa catcgaatgc
    63301 tcctttggtg attgagatgt atttttcgcc catttggtga actgttgtca tcatttttcg
    63361 ttcagaatca aaaggaagct cgtttactcg tggatatttt tcttctaatt ctgcctttgt
    63421 atgataattc tcttcaacag cccttacgat ggctacttct gttggattac ctttgataac
    63481 aaggtcgtca tcttcttgct cgaccgtcac atctgtacat agtgcagcca ttttcaatat
    63541 ttccatcgcg ctgttcgtca ttgaatcttc gatattcgtt acttcatctt ctttgctcca
    63601 tacccggcgg acgcgcattt cattttgtgt cagcgttcct gttttgtctg agcagataac
    63661 attagcggtt cctagcgttt caactgctgg caactgtcgg atgatcgcat gtttcctcgc
    63721 catcttctgc acaccgtatg caagtgtcaa cgtaacaata acagtcaagg tttccggtac
    63781 agctgcaaca gccaaggaaa tcgaagtcat gaacatctca aggagtggtt ctccttgcag
    63841 ttcaccaatc acaaatacta aagccgcagc cgctaaagcg atcatactga ttcgtttgcc
    63901 tagctgattc aatcggacct gtaaaggtgt tttttgcata gtattttcgt tcaaaagatc
    63961 cgcgatctta cccatttctg tagccatgcc aatggcagtg acgatcgctt tcccacgacc
    64021 tgcagcaacc gtacatcctt taaataccat attaagttgg tcacctaatg actcatcttc
    64081 ttcagcaata aatgctgcat ctttttcaac tgcttcactt tctccagtca acgccgattc
    64141 ttctacacgt aattgtgatg cttcgatcaa acgggcatcg gcagaaatag catcaccgga
    64201 ctctaaaaca agaatatcac ccactaccag ttgctctgac tcaacagtgg tcactttgcc
    64261 gtctcgtatg acagttgccg tttgtttatt catatcttgc aaggattcca gtgctttttc
    64321 tgcgtttcct tcttggacaa tcgccaatac cgagttgatc acaacgatag cgatgatcag
    64381 cagtccttca aataaatccc catgttctgt agcaaatgcg gtataaaagg aaatcaccgc
    64441 tgctacaaga aggatgatcg ttgtaaaatc ggacaagctt cgaaagaatt ttttgatcat
    64501 cgattctttc ggcgcctcat cgaatttatt caatccctgc ttttttcgcc gttcatccac
    64561 ttcttgtgaa gacaacccag tatctggatc tgcttctgtt accttcttta cttcttctat
    64621 tgtctgcttg tattcttcca tcctattcct cctttaaaat cttcgtacaa aaaaaggtac
    64681 ttattttact ataacatcct ttttttattt acttcaaata aagggcttat cgattatttg
    64741 ttaagaaagc gtaaaattta aagtgcttat atttgcaaac gctaccaaag gatgctatta
    64801 tatagatgta aggaaggagg ataccatcaa atagacagaa gaatttctcg atagaacagt
    64861 gtaagcaaac aaagttgtta gaagtacagg tttcacccag acatgcgtgt agagtacaaa
    64921 cctataaata gtgaatgcgt atggatgttt ggatcacgaa gtatccgaaa caagaagtaa
    64981 tgcgatacgt aacaattgaa taacgtaaac gtctgaaaat tgaataggaa caagagatgt
    65041 ttcaaatgtt gaagatgtaa cgaaaacaaa atttttgttt aacatgaaga aattcaacac
    65101 cgtgctttgc atgtatgatg gaaagttaca atcatttctg tttatttgaa tccttccgag
    65161 gtacaagaaa gccgagctta tcgagagctc ggctttttct ttgtcataaa atctttgtaa
    65221 agcttttttg ttattcaaaa ctagcggctt ttcataacct tatttcacga acatcgcatc
    65281 tccaaaacta aagaaacgat agcgctcttc aatcgcatgc tggtaagcag ataaagtcaa
    65341 ttctcttcct gcaaaggcac taactaacat gactaaagtc gattttggca aatggaaatt
    65401 agttgagaaa gcttctacca ctttgaattc atagccaggc gtaatgaaaa tatcagtcca
    65461 tccgctgtct gctttgattt ctccattaaa tttcgttcca atcgtttcta atgttcgaat
    65521 tgaagttgtt ccgacagcca cgattttccc gccattttta cgtacttggt tcaatcgctc
    65581 agccgcttct tccgtcaaac ggtagaattc actatgcata tggtgttctt caattttatc
    65641 tacgctgaca ggtcggaatg tacctaaccc tacatgtagt gtcagataga ctaattcaac
    65701 accctttgct tcgatctcat caagtaattc tttcgtaaaa tgcaatccag ctgttggcgc
    65761 tgcagcagaa ccattttctt tcgcatagac agtttgatag cgatctgggt cttccagacg
    65821 ttctttgata tatggaggca atggcatttc tcccaatgat tcaagaatct ctagaaaaat
    65881 tccttcatac tcaaagtcaa tgatccgtcc gccgtgttcc aattcttctt tcactactgc
    65941 ttgcaatctg ccatcaccaa aagagattct tgtcccaact ttggcacgtt tcgcaggttt
    66001 gatcaatgtc tcccacgtat ctccttgtgt attcgttaga agaaggactt ctaaatgcgc
    66061 accagtatct ggtttttctc catataaacg agcaggcaga actcgtgtat tattcataac
    66121 tagcgcatca ccaggctgta actcttcaat gatttcatgg aaatgtttat cttccatctc
    66181 tccagttttc cgatccaaaa caagtaaacg agaatgatcg cggtctttta atggtgtttg
    66241 tgcgattaat tcttccggca agtcaaaatc aaaatcttct gttgttaaca tatcgtcact
    66301 cactttctat catgtgcttc atcattctat catgtttttc gttctgttgg caaaatcagc
    66361 actttttcac ttctattttt tgttttccta aataaaaaaa ctaccctatt tgtatttttt
    66421 cattgtttta ggcatactag taatagatag ttcttcattg aaaatagaaa aagaggtggc
    66481 accatgattg aatttcaaaa tgtgtcaaaa atttataaag gtggaaaagt cgctgtagag
    66541 gacgtcaatc tttcatttga aaagggtgag ttcatttgtt ttatcggtac gagcggaagc
    66601 ggaaaaacga cgacgatgcg gatgatcaac cgaatgactg atccaacaca agggaaaatt
    66661 ttgattgatg gaaaatccat ccaagaaatt gatccagtgg aattgcgacg tcagatcggc
    66721 tatgttatcc aaagtattgg attgatgcct cacatgacga tccgtgaaaa tatcactctt
    66781 gtccaaaaat tgttgaaagt cagtcaagaa gaacggaata aaacagctga aaaaatgatt
    66841 gatcttgtgg aattgccccg tgagatgtta gatcgctacc cacatgaatt atctggtggt
    66901 cagcagcagc gtattggagt cgttcgtgcc cttgcagcga atcaagatat catcttgatg
    66961 gacgagccat tcggtgcgct tgatccaatc actcgtgatt ccttgcaaga ccttgtcaaa
    67021 gatttacaag agcgtttggg aaaaacgatc gtatttgtaa cccatgacat ggatgaagcg
    67081 attaaactag caagccgtat cgccatcatg agtgaaggcc gtgtgatcca atttgataca
    67141 ccagaaaata ttttacgtca tcctgcaaac gatttcgtag aagagttgat tggagaagat
    67201 cgcctactac aagccaagcc aaatgcgaca agagtcggcg aagttatgct aaatacagca
    67261 atcacgatta caccggaaaa atcattacaa gaagcaatca aactgatgag ggaaaaacgt
    67321 gtagatacgt tgttagttac cgataattcg aatgtattaa aaggatttat cgatgtagaa
    67381 acgttgaata aaaaacgagg aaaagtatcc agtgtcggcg acattttgaa taaacatgtt
    67441 ttttacgtaa aagaatccgc ctatcttcgt gataccttgc aacgcatttt aaaacgcggg
    67501 ctgaaatacg tgccggtcgt tgatgaacag aacaaagtcg taggtatcct gactcgtgca
    67561 tcattagtgg atatcgtcta cgatgtgatt tggggagaag atcccgcaac tactgatgtt
    67621 gcagagtcag ctactcctga agaatctaag gaggtctgac ctatgcagca gttttttcat
    67681 cagcacggtt cagaaattct ctcaaaaggt tttgaacatc tttatatttc aggacttgct
    67741 ttgatcattg gtatcatcat tgctgtaccg cttggtgtct tactgacacg ttttccaaaa
    67801 gctgccacga ttgtgattgg cttaaccagt gccttgcaga caatcccttc tcttgcttta
    67861 ttggcattga tgatcccgct atttggcgta ggtaaattac ctgcgattat cgcactcttt
    67921 atttattcat tattgccgat tttacgtaat acgtacattg ggatgaaaaa tgtcgattgg
    67981 aattatcggg atgtggcaaa aggcatgggg atgactgagt tccaatccat tttttccgtt
    68041 gaattgccca ttgctatgcc aacgatcatg gctggtattc gtttagctgc cgtttacgtg
    68101 attgcatggg ctacacttgc ctcttatatc ggcgctggtg gtttaggcga tttgattttt
    68161 agtggactga ataattacca accagatctc attttggcag gtacgatccc tgtaacaatt
    68221 ttagcactat tggctgattg gcttctaggt ctcttggaac accggctgac accgatcgca
    68281 cttagagagg agaatgaaga atgaaacgta tactaaaaat gaccatggtt cttttctctc
    68341 ttcttatact tgcgagttgt tccttccctg gtcttgccag caatacagat gaagacacca
    68401 tttcgatcac aggtggaatc acttcagaag cacagatctt agcaagtcta gttgccggaa
    68461 tggttgaaca ttatacagat aaaaataccg caatcatcaa caatctggct actacaacta
    68521 tcaaccatca agcgatgatg aacggagatg cttcaatttc agcggctcgc tacacaggaa
    68581 cagatttaac gactacattg aaccttcctc ctgaaaaaga tccgaaaaaa gcgtttgcta
    68641 ccgtgaaaga tgaatttgag aaaagatacg gtcaaacgtg gttcccgtcc tatagttttg
    68701 aaaatacgta tgtctttctt gttcgaaaag acaccgcaca aaaataccat ctatctaaag
    68761 tcagcgatct gaaaaatgta gcggatgaat tagtagctgg tgtagatact tcttggatca
    68821 atcgtaaagg agacggttat gacggcttcc aagaaactta tggcttctcc ttcaattcga
    68881 tattacctat gcagattgga cttgtttatg acgcagtcga agcaggaaaa atggatattg
    68941 ttcttggcta ctctacggat ggacggatcg ccagttatga tctagtcatg ttggaagatg
    69001 atcgtcaatt cttcccacca tatgatgctg ctccagttgt agacaacaag atattagctg
    69061 atacgcctca tttaaaagaa gccttaaata agttaggtca taccatcagt acggaaaaaa
    69121 tgcaggaatt gaactacgaa gcagataata atctagtgga accatcagta gtagcacaac
    69181 gtttcttaca agaaaaccac tattttgaag ggaagtgaag caattggata aaatgaatgt
    69241 ctttcaacaa tttttctatt atttccagga aaatggctcc tatatctttg cacaatttat
    69301 ccgtcacttc ctgatttcta tctatggtgt cctctttgca gcagttgtcg ggattccggt
    69361 tgggatcatg atttctagga gacgtaaatt agcaaactgg gtgattcgtt tggcgaatat
    69421 catccagacg atcccttctc tagccatgat ctccatttta atcattggat taggcctggg
    69481 agtcaatgtc gtgattgtta ctgtattttt gtattccctc ttaccgatca tcaagaatac
    69541 gtataccggg atgatccaag tagacaaaaa catccttgat gtcggcaaag gcatggggat
    69601 gactgctaga caacgactat tcatggtaga gttaccactc tctgtttctg ttatcatggc
    69661 aggcataaga aacgcgttag ttgttgccat cgggataacc gcgatcggtg cattcgtcgg
    69721 tgctggtggt cttggagata tcattatccg aggaaccaat gcaacagagg gcacatcgat
    69781 tattttagca ggtgcattgc caactgcatt aatggcgatc atcacggact ggcttttagg
    69841 tattttggaa agacggctag atccagccag ccgtacatca cgctgatgtt attgtaaaaa
    69901 acaaaagctt ctgcgaccat ttgttggtcg caaaggcttt tttagttctg tcatttttaa
    69961 ggcaataatc cactctgatt cttccctcct tatgggcaaa gagttgcttt tcttagctat
    70021 ttatccattc cgtatactcc aacttcatac tgccaacggg ttcatatctg catccaccga
    70081 ctaagttttt ttcttatccg ctaaggcatt ttgccaatcc attttcccat cactcgtcat
    70141 ggaattcata ccacatgatc tctaaatact cgtctgcttg ttctttgaat tgggcatcaa
    70201 ttcttacatc ctttggcgtg gctttcattt aagccttttc catcagtcct cgattgttcg
    70261 atgccttcag ctgcatcatc aaaactggtg actgccgtat cggtaacttc gatcatcttt
    70321 tatacaatcg ggaacttgcc atcctcatcc ttggctaaaa acatatctag ccattccagc
    70381 ttctcgccat gggaggtttg cggttcctaa aaccggtaca cgattcttat ggactgcatc
    70441 cgtcgcaact agacttggcg atacgatgat ccttcaccag aagagcaccc ccgtaaacca
    70501 attgatcgat atattgccaa taagaaaaga cattggcgta aaagatatcg atccctcgtg
    70561 atgcattgcc gttgatactg ctattcatgg ccgaaagcgc cacgacttta acttcttccg
    70621 tttgagtgac attcatgttc ggcaatgtcg ctttggatac ccgctctgct aaaggaacga
    70681 cactggtatt gtatttcgta tctgtatctt ttccaaaaga ccatgccaac gaatcctctg
    70741 ggaaccaata agacgactcc ggttgattgt ccattgcttt tttcaacata cttttggctg
    70801 tttctcgata aacgacattt ccaattgtct tcgcctcact ttggcgataa tcgatggtcg
    70861 ttgccactgc caacactaac aacgatgctc caattttact gattttcctt gtcacacttc
    70921 ttcctccttg ttttacataa acccgtcaac attccattcc taaactcaaa caatgaccct
    70981 tcaaaggtgg agatatagga acaaattaaa gaaatcatgc aataaatcca agaaggttgg
    71041 ttttagaaca aaaaaagagt ctcctttttg gggagactct ttgttaacta tctaaaaagg
    71101 ttggctcttc tcacttcact cataatgtgt ccaggcagaa taaatttcta caactttatt
    71161 ttcctcatca actttgtaaa ccactcgatg ctgtctattc agcctgcgtg agtatctgcc
    71221 ttcatgtgta ggtctcagct tttcaaaaga ctgcgtgggc atatatggat cttccttcaa
    71281 tgtctgaatc acttcttcaa attgtttctt taaattcgtt tgcttgattt ttcttaaatc
    71341 cactttcgca gagtttttga ttgcgactgt atagttactc atcaaagagc ttcccaatca
    71401 atatcatcga tattcgtaaa gctactgtcg tctttttcgc ggtctcgtac tttatctaaa
    71461 gtacctgttt gctcaagaaa aagggtttct tgaatcgctc tccagtcttc cagcccaatc
    71521 aatacagcat tgtttccact acgatcacta ataatctcga tttcagtatg attttcattt
    71581 acctctttca ataactgata aaaattttta cgagcagtgc taggattcat cgttgtatta
    71641 gccattgtct ctcctccttt ttctgatata attatagcgt acttgattaa gtacgtcaaa
    71701 aaaatacaat aaagcactta aattcagcaa acttttcctc ttttttacat agagaaaaag
    71761 accccttttt agggaggatc tttgtccacg ctcacttctt actcctttac gactagcggt
    71821 agccaaacgt aaagtaattg gtaacaacgt atcatcttta gcaaaggtcg aatccaaagc
    71881 ctgtgagtct atgcaacaac accaatacac gaaggttcct cgttgcagtg tgacttgatt
    71941 gatatcttga ataacctgag gatacgcacg aaagatttgt aacggttatt caaaactgat
    72001 tttggccgtc aagtcttcta ctgcctcaaa aatgattgcc tctttcgtct cctgtcattc
    72061 atgttcacta tcgatttgat acttgcttag accgttgggt ttgtgaatca ccaattaaaa
    72121 atggcactga gttatggtaa caaaaaatgg tcacttgacg aggctgagat tcctacttat
    72181 gaagacacat ggcacttaag agtcgaggta aaggaagaag ctgaaaatat aggaggctgt
    72241 acattatttg gcaaaggatt cggaaattat gatcaagaca tcaaaatcaa aatgttttac
    72301 agctaaatag gaagaataat aacatttcat acgctgttcc catctttaat ggcttataga
    72361 gggcaccttt agctgagaat aagacaaaac agaccctcta gcttcaatta agagctagag
    72421 ggtctgtttt tgattttaaa acttggttta tttcttcaaa tggtagctat aggtgctgac
    72481 aacgatattt tcacgccaag acaatcgcaa tcttaaccgc aaacttgttt ggttatggag
    72541 aatattctgc cagcgtctgt ttggtacaaa ttgaggaatc aacacagtag ttgtatagtt
    72601 ttgtttcgct gcatttttac ttacgatatc tacataacgg ataattggat tttcaatcga
    72661 gcgataagaa gagtggacga ttgaaaaacg gacatctggg aaatgctttt taaattctgc
    72721 ttcgatttct ttttcttttt cgacatcttc gtctagcgaa acatgcatcg caacgacata
    72781 atcaccgatc gaacgtgcgt aattcaacgc accgatattt acccgtgtca cattcccgac
    72841 taatacaaga acggtattcc cgtcataatc atgtagtttt gcatcatttt ctaaacgaag
    72901 ctgttctgct acttttttat aatgatcatg gatcttataa aaaatgaaca taacgattgg
    72961 cataatgatg aagaaaggcc aaatgtcccc taaccgatag acaaacaaaa tcgcaatgat
    73021 tgcatatgag atgaacgcac ccgtaatatt ggcaatcgat ttgcttaacc atctttttcc
    73081 ttcttttttc catttgacga ccatccctgt ttgagataaa gcaaatggaa tgaacacgcc
    73141 gatagagtat aacggaatca agcgttctgt tgagccatgg aaaataaaca gcaatacgat
    73201 cgaacctagc gctagtgtga taatcccatt ggaatagcct agtcgatccc cacgatcttg
    73261 atacatgtga ggcatgaatt tatcttttgc cagattgtat gcaagaactg gaaaagcaga
    73321 aaaaccggtg ttagcagcaa ctgctaaaat cagcgctgta gcaaattgaa ggacgtaata
    73381 caaaatacca tgtccaaaga cagctttacc aatttgagac aaaacagtga cttctttttc
    73441 aggaacgatc ccataccaat agttgataaa tgtgatcccc acgaagaaga aacctaagat
    73501 caatgccatc atcgtcaatg tagcagcggc attttttgct cttggcttct taaagaatgg
    73561 tacagcatta ctaatcgcct ccaccccagt taaagaggaa gaaccagaag aaaatgctcg
    73621 taaaatcaaa gcgatagaaa cacccggcac agccgctcct ggaagtgcag tagcttgcaa
    73681 tggcacagca cctgtcacga tattatataa accaacaacg atcagtaacg agatcacaag
    73741 gataaatgtg tacaccggaa ataataggaa cgatgcagat tccctcagcc cacgaagatt
    73801 cagcatcatc agtagcaaaa cgattgtcac agaaatagcg acttgatgac cgtagagtgc
    73861 tggcaccgct gatgtgatcg cttcagcacc tgctgagaca gatactgcta ctgtcaacat
    73921 gtagtcgatc aataaagagc caccggaaat caagccagca ttctttccaa gattctcact
    73981 gctgacgaca tatgctcccc cgccatgagg ataagcatgg atgatttgac gatatgatag
    74041 cgttaatgaa atcaataaaa taataacaaa agcggcaatg ggtagcgaat accagatggc
    74101 tgcagcagac aaagcgacta aaacgacaac gatttgttcc gtaccatatg caatagatga
    74161 taacgcatca gacgacaata atgccaaagc agcaaatcgg gttaatttgt gctcatcatt
    74221 ttcagcagat tttaaaggct tacctactaa caaccttttt aagtaatcca atttctaaac
    74281 tcctttacac acaattttac ttcaagtttt atagtcgaaa atttatagta aacaagatgt
    74341 tatagtacgc tcactgacct taaaagtcaa taaactttaa gaaaataatt ggaaaaaaac
    74401 agaaaaatat caagcgcttt ttcaaacgct ttaatacagc aaaaatctaa cattttttta
    74461 tgggcttttt ctttgaattc tataataaaa ggatagaact tgacttagaa aatcaagttc
    74521 tatccttttt gatatcttaa cttttggaag agagatgtaa ggctgctttc tacccatcat
    74581 tcttactact cgaagcatag cgcagttgtc acaacctctt gataaacggt gtttaaaagc
    74641 ttgcatctgc ggtaataagc ccaaccaaat taaaaacata agaagctgtt tttatttggt
    74701 cgttcttatt acttgatgca gagcagcttt tttacaacct cttggtacac ggtgttcaat
    74761 cagctgaccc tcggtattag ctcaaaaaaa cggcaaaata tgaggaacta tttttccatt
    74821 tttttctcta atactcaggt caaaacactg atttcacaac ctcttttttt aatgaaaaat
    74881 atggctctca tgcgccgctt ctttgaattt cgtcattttt tcttcgatga acggatagat
    74941 tgccgttcct acgattccaa agacaacaaa caaaccgatc atgatccaga ttgctttcgt
    75001 agcatttggc caataaattc ctcctgcaga ttcccgtaac aagtttaccg catgagtaaa
    75061 tggcaagaac ggattgatca tttggaagaa tttccctgag acttggatcg gatagttccc
    75121 gcctccgcca gagatcgaca gtactaagat gataatagcg atccctttac cgacattatc
    75181 gaataatgca gccaagacat agacgatcat catgaacgct aaggcgatca agacagcaaa
    75241 tgacacgctg tagaccggat tttggacatc tactcctagt aagaaaatat tccctaatgt
    75301 aacgatcaag ctttgcgcaa tcgccattgt caaaaatgtc agcatacgag caacgaattg
    75361 ttctcgtttc gtaaaattgt ttcggtcttt ctttcctaaa tagtattctg tcgtagcaac
    75421 acttgaaaga agaacagcgc cgacccacag acatagtgca gtataaaacg gggtgctagc
    75481 tgaaccgtta tttgcgattg gataaagcgc attggtttgt agctcaacag gttgtgtaaa
    75541 gaagtcactt tcttttgttg cgtctagttt caataatttg atgacttcgc ctagatcgac
    75601 ttcttgttcg ccttttcgga tcgcgtctgc tgctttttgg ataccagctt tgatatttgg
    75661 ccagtcattg ttgatcaagt ccgcagcagc gttcacagct gtttcgactt ctggcatttt
    75721 ttcatttact gttgtcaatg taccggtgat ttcatttttt agtgctggcc aatcattttg
    75781 gataaaatca gcggccaatc ctaatttttg ctgaatgatc ggtaattcat tgttgtatag
    75841 gtctgcccct tcattgatgc cattgacgat ggtatccata tttccattca gcattgtatt
    75901 ggcatcatgg atttcttgtc caattgcagg catttctttt tgatattttt ctaaaattgc
    75961 cactgcattc gttacagttg ctttcgttga gctgagaagc gaatcaaagt cgatctggtc
    76021 cgctttgctc agtgcgcctt gtgcagtccc aatcgtatcg atgacttggt ctagtagaga
    76081 agtgacagtg gaactgattt gacctgtatt gataccagca atgatgccat tgatattatt
    76141 aacaacggca tcaatttgat ctagcaattc atcaatagcc ttcgtgtctt cattttgaac
    76201 agctgtgttc aaagcagata ggcggttttg cagattgctt agcaagggtt ttaattgatt
    76261 tagctgatca ataattggct gtaatttcgt atttccagca cttgtttgca atttagtcag
    76321 gaactcgacc agtttatcga ttgcattttg ttgttgagat attccgtcaa taaagccgtt
    76381 caatacattg ctgatgtttt tcttttcatc atcagtcagc ttaccatctt caatagctgt
    76441 tcttacagta tctattaacg aacctgtcgt tgttgccact tgtccaattg acttcaatgt
    76501 aacatctaca ctgtttgtga tgcttggtat cgcatctttc agctgagagg cacctgattt
    76561 tgtttggttt gctagatcac ttgcctgatt ttctaaattt ttcacatctg gcaagatatc
    76621 ttgtacttgt tggatgatcg tcaagccttg ttttgcttca tcgattcctt ggttcattgt
    76681 ctcttggatc gaagcaaaat cgccgtcgat ctctgctagt tgcttaccgg cattttggat
    76741 ctctgggatt ttttcttgca aatcaaggat gatttttgct tgatctttca actcaggcat
    76801 tttgtcattc aatgcaacga ctttttcacc catctcatct actttaggga tgtaatccac
    76861 gaattcattt gctttattca gtttttcctt gatttcaggt agttgacttt gtaattcgag
    76921 gacttgtttt gtgtagccgt cgatcgtgtc caaattttca tctgttgata aaatcatatc
    76981 tttaacttta ttgatactta ctaggttcgt atcgatgtcg taacctatct cattgaatac
    77041 tttcaataaa gtcgaactgg ctgttttgat aaattcattt gtgatttgcg attggatcga
    77101 acttgctcct ttatcggtga ttttcggtgc aatcgcattg attttttcat ttaccgtata
    77161 ttcgattttt ggtttcttga tatcaccact agtaaaactc agcagatctt ctgagaaatc
    77221 ctttggcaag taaatacctg cgtaatactt tccggaacgg actccgtctt ctaattgttc
    77281 ttttgaatca acgaattgcc atcctaattg tttattttta tgaagcgatt cgatgacttg
    77341 ctccccaatg gccacttctt taccttgaaa ctctgctggc ttgtctgcac tgtaaacagc
    77401 aattggcagc tctccagtat ttccatatgg atcccataat gctttgatgt taaaccacgc
    77461 atatagcgaa gggatgatca tgatcgcgat gatcagaagt gttgcaatcg ggtttttgaa
    77521 aatccgtttc cagtcaagca aatataatcg aagcgtattt ttgattgact gcatagttct
    77581 cccccttctt cttttttcag ctgaattact ttaccatgta tttcatactt ttttcaatcg
    77641 gcttttttca aaaaagtcga atttttggac atatcacggc aattgtctca ttgtaatctc
    77701 ttacatattg agaaataatt aaggattaca gcctttactt tatcatgaaa taaaagggaa
    77761 agataatttt atgccaaagt ttttatctat tatacgtata cgcaaaaaag ctaaaacatt
    77821 ttttgtcttt gcccctacaa aaatgtttca gcttttttcg ttttttattc ctgtatttct
    77881 tatttttcga atgacaggat gattttccca gcaggaggat gtccttcaac tagctgatgt
    77941 gcttggatga catgcttaag tgaagcagac agattcattg aaataaaaac atgaaaggct
    78001 cctttttggt aggctttgat tatgccagca aaggcttctg catctagata ttcctttctt
    78061 ggaacaaacg attcataaaa gccttccttt ttttgccgca aatctagatc tgggaggtca
    78121 tttaatgcaa catacctgcc gcctggtttc atgatctgga tgccggtttc tcctttgata
    78181 cttcctttcg tcgcatcgat tacaaggtct gcctgatcag caaattgcag acctggattt
    78241 gttttatcat aagcagcaaa tgctgaagct cctaattttt ttaccttttc ctcattttta
    78301 ctggatgcac tggttaagat tcggattccc ttttcatgga gaagctggat cagacttgac
    78361 ccgaccgcac ctgatgctcc tagtatcatt acagtatctg tcggctggat ttccgtcaaa
    78421 tggttgatta gattataagc cgtaattcct ggcgtgacca ttccggctgc ttcttcccaa
    78481 ctcattttat ctggaatttt tgctactttt gctgatggca acacgacttc ttctccgtaa
    78541 gtcccgccga ctgcatggac agctactcga tcacctacat gaacagttgt gacatctgaa
    78601 ccaatctctg tcacaatacc agcgccatca ttacctggta cataagggaa tttcaatgtt
    78661 cgtatctctt tcatttctcc taaacgcaag gcaacatcat aaggattcac actgaatgct
    78721 tttatttcta cacgaacatg tccagaaccg acttctctgg gatgagcatc gatttcttca
    78781 aaaacatctt gtgcttctcc gtaaccgtta attgcaaatc ttcgcatggt caccatctcc
    78841 ctttttcttt aagtttacgt ttatgccttt attttgacaa aaaaaatgtc ttttcgtttt
    78901 ggcgcatgat tcaaaacaaa atagttgtct tttttcgcgt aacgtgttaa attatttatc
    78961 taaaagcgta aagattacga tttacgctaa taaaaaataa ttggaggaat ttgttgatgg
    79021 ctaaaaaatc aaagattgcc aaagcaaaaa aacagcaaga aacgattgaa cgctatgctg
    79081 ctttacgcca ggaattaaaa gcgcaaaaag attacgaagg cttgcgtaaa cttcctttag
    79141 atgctcgtcc agaacgattg aaaaatcgtg atttaatcga tggacgccct cgcggataca
    79201 tgcgtaaatt cgggatgtcc cgtataaaat tccgtgaatt agcacataaa ggtttgattc
    79261 caggagtaaa aaaagccagc tggtaatcca agtctggctg taatgtaaag gaggagggcg
    79321 catggaccac tccttggatc atcaaaaaag atacgttcgc ttcttctagc agaaatgctg
    79381 gtactagaag cagacgtatc tttttttacg cacagctggt ccttttttat atgtaaaggc
    79441 tgcatggtta cttcgctata tttatttagt aacaggagtc agtgcttcga tacccacact
    79501 aattttcaat gctcggtcaa cttctgccat aatgggtcga tccaaatggc agaccttttc
    79561 tttcaatctt gatttatcga tcgttctgat ctgctccaac aggataacgg agtctctttc
    79621 tattcctgtt ccagtagctt tgatcccaat gtgagtcgga agcttaggtt tagccatttt
    79681 tgccgttatg gccgcaacaa tgatcgtcgg gctaaaatgg ttacctagat tattttgaat
    79741 aaccaaaact gggcgtgttc cgccttgctc cgatcccacg actggagaaa gatcagcaaa
    79801 ataaatatct cctcttttta ccataggatc ctccttattc ttgatattct cttggtatcc
    79861 gctgtcctaa tccgcaagcc acttcatagt ggatcgtacc taattgatct gctatatcct
    79921 gcatagtgat ttctttgtct gcatttttgc cgataagcgt cacttttgtt ccgacaggaa
    79981 attcttgagg caatcggatc atcagttgat ccatacatac acgcccaatc gtttcacagt
    80041 agttcccttc taccaataga gaaaaaccct gcatctttcg tggccagcca tctgcataac
    80101 cgatcggtat cgttccgatc cactctgctt caggagtgat atatgtttct ccatagccga
    80161 ttccttcacc tgctgggagt tttttgactt gaatcaattc agaaaccagc tcaagtgctg
    80221 gctgcaatgg ataaacttcc ggtagtgcgt gcccagaggg attaagtccg tacattgcta
    80281 ctccataacg aatcatattg ccgattgttt catcatgcca taaagccgtt gcactattgc
    80341 tgacatgtac atatcgagga agttcgggaa gttttttcag cacttctata aagcgttctt
    80401 tttgcagatt ccaatatgta tcatctgcct gatctgctgt agaaaaatgg gtaaataaac
    80461 cttcccattc taatacacga ctttctttga tgaatgcagc tgcctctttt acttcttcag
    80521 gtgtacaaaa tccgatacga cccatcccag tgtctgcttt tagatgaatc gaaagaggcg
    80581 tctctagctt acttttctct aaaacagcag ctgctgcttg caaccattcc cgtgtacctg
    80641 cagttactga taaatcatga gcaacagcta cagtcagtgc gtcgtaagga accatattca
    80701 agataaggat gggctgagta aatccagctt ctctcaactc gactccttca tctaaatttg
    80761 aaacacaaaa gcccgaagct ccacctgcaa ctgctgcctc agctgtttca atagctccat
    80821 gaccgtatcc gttcgctttt actactgcaa acaattcttt tccttgcgga agtcttgcaa
    80881 cttcatttgc tacattttca gttatagctt ttttatgtat cactgctttc gttgggcggt
    80941 gccaagcgac aaccatcgtt tttccttctt tctagtcttc caaaataatt tgtgcaaaag
    81001 ctgtttcatt tgtatgagta atactgaccc atacattgcc ttcatgaggt gaacgcgtca
    81061 caacaggctg accattttca ttttttaaaa tctcgacatc ttgaaaggta acttttccga
    81121 tcccagttcc ccaagcttta gagaaagcct ctttgcaagc gtaacgcccg cctaaaaatt
    81181 ctacttggcg atgaaaaggc aaggactgga atagtttcat ttcatcagag gtcaagattc
    81241 gtgcaatgaa ttttggtttt tcttcgatca gccgagtgat cctaggaagt tcgactgcat
    81301 cgatcccaat tccttttatc atatgctttt ctcttttcaa taaatttaga acactctcat
    81361 tctaccaaat tataaccatt ttgccacaaa taagtccaca aataacaaaa atgaaagttt
    81421 ctcttaaaaa agagaaactt tcattttatg tttaatcgtt atttgaacgg attacaaatc
    81481 cgcgtttttt atcattgtga cgtttaccgc catctttttg gctgttatta tttttgctat
    81541 aaccgccttt tgatttgttt ccacgataat taccgccgtc acgacgatta cggttgtttc
    81601 cgccaccacg tttgccattt ttattgtatc cttttttctg cattggcaat gggcgttctg
    81661 gtgtgatctt cactggcact gcatcagctg gatcttttgc tgtcgttttt agcaaaagtg
    81721 caactaaatc ttgtgcagaa tattcttcta ataatttatc tgctgtttgc aggtatttat
    81781 ctagaccatt ttctgcaagt tttgtttcga tttgttcaac tgcagcacct aattgacctt
    81841 tgaatgcttc tttttcggtt ggtggacgta aagtcgtcat ccgttttttc gtcaagtttt
    81901 caatcacatg caagtagtcc atttcatttg gtgtaacgaa agtcacagac ataccgcctt
    81961 ttccggcacg gcctgtacgt ccgatacggt gaacgtagct ttctggatct tgagggatat
    82021 catagttgta cacatgagtg acacctgaaa tatctaatcc acgtgcagca acgtctgtcg
    82081 ctacaaggat atctaaatga ccgctcttaa atgaacgcaa tacgctcatt cgtttttgtt
    82141 gagacaaatc gccatggatt ccttctgcac ggtaaccgcg tgcttctaat ccgcgtgcca
    82201 attcgtctac acggcgtttt gtacgtccga agacgattgt taattcaggt gtttgaacat
    82261 caaacaagcg agtcatgata tcgaattttt cgtattcttt tgcgcggaca tagtattgat
    82321 cgatcaagtc agctgtcatt tctttcgctt tgatcttcac atgatgtgga tttttcatga
    82381 atttaacgcc aatattcttg atagctggcg gcatcgttgc tgagaaaagt aatgtttgtc
    82441 tttgatcagg aacttgtgaa atgatttttt cgatatcttc caagaagccc atattcaaca
    82501 tttcatctgc ttcatccaaa actaatgttt gaacggttcc aagtttcaat gtgtggcggt
    82561 taatgtggtc aagcatacgt cctggtgtac ccacaacgat atgtggacga tctttcaagc
    82621 cacggatctg acggccaata tctgctccgc cgtaaacggc ttgtacacgt acttttttgt
    82681 cgcgccctaa acgatataat tcttcttgtg tttggatagc taattcacgt gttggtgcga
    82741 taactaaacc ttgtaattcg tgacgatctg gatcgatttt ttctaacatt ggtaatccaa
    82801 atgcagctgt tttccctgta cctgtctgtg cttgtccaat aacgtctttt ccagctaatg
    82861 ctagtggaat cgtttctgct tggataggcg ttgcttcttc aaaaccagcg cgttcgactg
    82921 atttcaacaa ttctggtgat aattctagtt ctttaaattt caaatgaatc ctcctatatg
    82981 gttgttaact gaggatgtag cttttttgct accccgtcga tttgttcggt caaagtgtat
    83041 tgtttctttt tgacagtggc cacgcacgtt tttatccatt cttagcccct gtggcaccac
    83101 cgaaaaatca ctttgcgaag tttaggcatt agaaaagcca aacgggtcgc gttcagcttt
    83161 atcattatta tgacatctgg cttcaacaag ttgtttctat ggcaaaacca gtccaatttc
    83221 tcttcatctt tcggcaatag gttattctaa catagttttt cgttttctgc aagtttacag
    83281 atttttattt catttgctga agttgttcta ccacttcaaa taagcccatg ctgttgctgc
    83341 cttttagcat aatactatct gttgcttcaa gattttgttt gatagcatca atcaatttat
    83401 tcttttcttt ttcaaaatag aacacaggta gttgggggta tttttggttc aattcttctt
    83461 ttagtgcatg catttgttcc ccataaagga aaatcatggc gaatttctca ggatcgatat
    83521 gttcactcat ttgcgcatgc atatttaaag actctttccc taattccagc atatctgcta
    83581 acacagcgat tctgcgtcct tttgttggca gatttttaaa agtatctaat accagtccca
    83641 tggctgttgg atttgcattg tagacatcgc tcaaaatagc tgcaccattt cctgccgtta
    83701 gccattcggt tctgttttga gtgatagaaa catgggcgag cccttggcgt atctcaggta
    83761 tggacagtcc aaagaagcgc ccgatcgtat acgcaatcaa agcattcgtt acattataac
    83821 ttccaggcag cgggatcgta aatgtttctc cttctactac aaaatcggtt tgtgcttttc
    83881 cttttgctgt cacttccgca tgaacatcgc catttccaat accgaatgtt gtgactgttt
    83941 gtactaatgt tgccgttaaa ggtgtcagca acggttcatc tgcgggcaca acgagtaatc
    84001 cgtctgatac aagtccgtca gtgatttcca ttttggcttt agcgattcct tctcgcgacc
    84061 caagattttc tatgtgtgct tcaccgatca ttgtgattgc tgcagctttt ggttgagcca
    84121 taagtgaaag ttccgtgatt tcattggcgt gatccatgcc catttccaaa acgatctttt
    84181 ctgtatcttg gggcatgtgc aaaatcgtgt aaggaagacc aatattattg ttgtaatttc
    84241 cttgcgtctt atatgttttg tatttttcag acaagacaga cgcggttaga tctttcgtgg
    84301 tggttttacc attgcttcct gtaattgcaa cgacttctgc gcctactttt tccaagtaat
    84361 aggttgccaa ttgctgcatg gctttcaaag gatctgctac aggaataact ggaaaatttt
    84421 ttggagcttg ttcaacaggc aagctccaaa atgaagcaga tgctccgttt gtgatcgctt
    84481 gctggataaa ttcatgtcca tctcgcgtac cagcaagagg aacgaataaa cttccttcgg
    84541 ttatcttccg gctgtcaaat tcgacattgg taatctttgt cagtgcagag cattcgctgc
    84601 ccacggcttg tgcaatttct tgtattgtta gattcattga gatctctcct aaaaaaggtt
    84661 gtgaaaaaag cgtttggcct aaacggcggg ttcgcatgac tctcacttcc aagtaaattt
    84721 ctctattcgt atttagaagc tagaatgaac gcggttacgc aatcactcca gcctcttctt
    84781 tatttctctt tattcatttt tttcaaaaaa agattgacgc tgatggtatc gattcattcc
    84841 taactggatc agttcttcaa tcaaatcacc gtatttcaag cccatatttt cccataagag
    84901 tgggtacata ctgaactccg taaatcctgg catagagttt aattcattca ggaataattc
    84961 atttttattt gtcaaaaaga aatcgcaccg gctcaatccg cttccaccta acatcgtgta
    85021 agctaacttc gcgtactctt gcgctttttg ataaacttct tctggcactt cggctggaat
    85081 ctgcatttcg attttattat tgatatattt tgcttcataa tcatagaatg ctacgtcttt
    85141 tacgacttca ccaggcaaag tcgtccgaac gtcttcattt cctaatacag caacttcgat
    85201 ttcgcgcgct tcaattcctt gttcaacgat tgctcgagaa tcatactgat aggctgttgc
    85261 taaagcattt tgcagctctt ctcggttttc tgcttttgta atgccgacac tagaacccat
    85321 attcgccggt ttgacaaaca tcggataaag caaagaacct tcacattgat caaatacttt
    85381 tttaggattt tctttccatt gattcttaag tactggtaca taaggaactt gcggcacacc
    85441 agcagcttgt aaaatatact tggtcatgat tttatccatg gcacatgcac tggtcaatac
    85501 gcctgcgccg acataaggca tattcaatgt ctctaagaag ccttggatcg ttccatcttc
    85561 cccgtttggc ccatgtaaaa ctggaaaaac gatggctcct tcttctttga tttcgcccgg
    85621 attgatcact tttcctgtaa agccttcctc tgtctgtcca cttgggtccc atgaaagatg
    85681 caagacatct ttgctggcag gtttttctgt tagtaatgga cctttgaccc attgtccttc
    85741 ttttgtaata aatacgagtt gaacttggta ataattataa taaatggcat ttaaaactga
    85801 aaatgcggaa agaatggaca cttcatgctc tgcgctgcgt ccgccatata gtaaagtaat
    85861 cttcaaaaga ttgtcctcct gtattctaaa tcgttcagtg atttactctc atattttagc
    85921 acaaaacaaa aataaacgaa taacttattt ataaaaaaac gagccagctt ctaaaagaac
    85981 gaacgtcatt tattttttgt cagctagatt atttgtcccg ttttttataa aaagttatga
    86041 taaagaaaag gagggatgat gatggcgcaa acaaatccac gcagccgctt ggcagaaatc
    86101 attcgagtgt tcatcaatta caatgtagtg ccaaactttg tacagcaaaa aaatcccgaa
    86161 caggtaaaaa aagcctttga agaactaggc cccaccttca tcaagatagg acagatgctt
    86221 tctgttcgag aagatttgct ttcttctgcc ttcactcaaa cattcaaaac attacaagat
    86281 agtgtaccta gcgacacatt ttcaacggtc aaaaagacga tcgaaacaga actttctctt
    86341 tcattatcag atatattcga tgatttttca aaatcgcctt ttgcctctgc ttctatgggg
    86401 caggctcatc gtgccaagct gaagaacggt gacgcggtag tcgttaagat ccaacaccct
    86461 aacatcgccg aagagatccg acttgatctt caactatttg aacgagcgat ccctttgatc
    86521 aaatacattc ctgaaactag cgtagttgat ctaaaagggg tactccaaga agtaaaacgt
    86581 tccttgatca atgaaatgga cttcttaaaa gaaagtcaaa atggcgaaca attctatcaa
    86641 aagaacaatg gctggaaaga gatccgcagc cccaagattt atgacgcttt ttgttcgaaa
    86701 aaagtgatcg tgatggaaga aatgtctggt aaaaacttga atcatttaat gaacgccgaa
    86761 aacaaaactg aaacttttat cactggaatt caaaacaagc agctgaaaca agaagttgca
    86821 aagcttttag ttgagaactt tatgaaacag gtgtttgatg atggtttttt ccatgctgat
    86881 cctcatccag ggaatctgct attccatgta ctaacaaaag aagaacagac acaagctagc
    86941 cgaaaaactg aaacagtcca tgaaaaagag ttcggttcct ttgcttttcg agcctccact
    87001 tctgcagaag accctgttgc gccatatacc atcaactaca tcgattttgg tatgatgggg
    87061 catctttctg caggacttag gcaaaaactc acacaagccg ttttagcgct gtatacgaaa
    87121 gatgcttatc gaatcgaaaa agcggtcctt cgactttgtc agcaagaagg ctcgtttgat
    87181 gaaagtcggt tccaccaaga actgacaagt tttcttgaac agtattatga ttctcctatt
    87241 gatgaaatca acttgcaaga agtattcaca catgtcgtga cgatttgtca tcaaaataat
    87301 ctgcagttcg atcgtgatat cacgcttttg ctgaaagcat ttgggacatt agaaggggtg
    87361 atccgcgtcc tcgatcctga agtttccctc atggaagtcg cttcgccttt tgctcaacat
    87421 tattttctca ctcatctgga tgtagaagat acattgaaac aaagcggatt agacttattg
    87481 gaagggatga aggctgcgcc taagatccct caacagctcc accatctatt agaaatgtgg
    87541 acttcgggac aaggaaaagt caatttggaa ttgaaaaagc aagacaaatt attgagcagg
    87601 atcgaaagta tgatcaatcg gcttgttttc ggtatgattt tagctgcatt gatcgtaggg
    87661 tcctccctgc ttgtccaagc agcacctgta gaaaacgcag aggtcgtttc cttattaggg
    87721 atatttacgt atgctattgc cgcattcgtc attatttttt tggcaatcga tgcattgatc
    87781 caaatgtata agaagaggaa aaagtagaaa aaggttgtga cagaaagaat cagcttcaag
    87841 aaataagaag gggttttaca taaattgttt ctcaaatttt tgtgaaaatt cggcttattt
    87901 ttcgaagagg ctacttctga aacaccgaga ataaaaaatg tgaaaaagct gttctgcacc
    87961 cagtaataag aacaatcaaa aagggaacat ggaacacatt tatcctgttt ccctgttccc
    88021 tttttgatgt attcgagcat aaataaaata ccttcgttac ctactatctt ctgtacttgg
    88081 taaaccttag tcaattgcct tttttaacgc ttgacgaaaa gacatatgat ctttctccgt
    88141 aaagggtttt ggaccctttg tccttatgcc agcttttctt aactttccac tatgataacg
    88201 ttgttctaga agcgtgccga tattcgcttc gtcaaattct tttccagagt gatggaacac
    88261 tcgcacaccc ttcggcaata caagtgaagc taaaccataa tcttgcgtga tcaaaaagtc
    88321 attcttctta accataccga cgattttata atccgcacta tcagctccgc ggtctacgta
    88381 gacgatttgg acatgattag cgtattcttt ggtcgtataa tggtccacac tggtcacgat
    88441 aaccacgtca agaccatagg attctcctaa ttttatgaca tcttctttta ctggagaacc
    88501 atctccatca atgaatagcc tcattctttt tcgccttctt ccataaatga taaacagtta
    88561 ctaatccgct cagtaaaaga ccaacattga tccattttgc caagataagc agttgtacac
    88621 tttctatcgg cactgattca tggttcagcg tgatcaataa tagatggatt ggctctccca
    88681 tttcttttgc taaaacaaac aaaagcgtca tcaataaaaa ctggtggatc acactccctt
    88741 tattttgagt agtatacttg acaacgatcc ggcgatccat tggaatagct gtatcagttt
    88801 gataatattc cttatttagc cagctgcctg ttactacgtg ccagaaacta agcagaaaga
    88861 cacctatcgc aaaaccattt ggaatcaaga ggaccaataa agaagaactc agtccaagca
    88921 attgcatagc caatggagac catgttaaac gtttcaaaat ccatggtccg actatttttg
    88981 ctgcaatcaa gcctgctgca taaggcaaat acataaaaat cgaaagtttt tctaatccgt
    89041 acagtatgcc aacataaaaa ctgctaaata aaaaaaggaa attccctagc attccattta
    89101 gaaaagtaaa cagatttagc cagcttggca acttccattc cttcttctgg tgtcctaaaa
    89161 caaacacgaa caagatgaac agcagcaaga aaccataaat agcaagatca aaaagccttt
    89221 catcgaataa caatcgggca tttctcaaaa gaaacaataa gccaaagaat atcaaaaata
    89281 aaacaagctc tcgcgtagct accagatttc ttttgatgtc tttgaaatcc agttcgtaac
    89341 gcggataatg actaaccgta tggtaggcca tgacgtaaaa aagtgtataa atagtaaagc
    89401 ttgctgcaat ctgagtcggt gcaggcaact caatcgcttg atacaggaga aatagccaga
    89461 tagctgcaaa taagtacttt ttcccatcca tattgataaa accttgcgtc ttttcgtgaa
    89521 aattgaccgt ggtattagca ggtacaagcc atgctgcact cagccctagc aaaacacttg
    89581 aaaaataaaa taaaggaaaa taaaatacgc ctaatattcc aagcaatgaa ccagcaccac
    89641 caatcagcag agcgatcatc agcaacgtgt aactatctag accaaatcgt agactgttga
    89701 ctaaaaatag tccagtcatc cgaaaggcat aaaacagcgt aaatggcaaa atcgtcagcc
    89761 aattatgcga gtctgttagt cccaaaaata aaagataagg aataaaaata accgtgttac
    89821 ttaagataaa gggcgttgtt tgtgtaaaat tacgcccaaa tcgtttgagt ttttcgaaca
    89881 tgacaatggc tccttaaaca gtcttataac taatctgctc ttcgatttta gcattaaagg
    89941 ggaaataaac caattttaca gaatatattc ttgtaaaaaa aaatacagaa agaagcataa
    90001 gcttctttct gcatttttac ggtacttgat tactcaagca gacaagccct tctccacaaa
    90061 agtcttgccc gacacccctg catccggcag gtgataaaga tagaaatcaa ttatcctgtg
    90121 cctccaaaat caattaggag ctctgtaaac cagagtcctt ctcgacccgt cgcataaatt
    90181 tactttaaca cagttcctat gtttatgcga gagctttttt cattttataa tcgaccattt
    90241 gaaatccttg accgatatct gaaataagtt cttctgtaat ctcaaatcct tcatgcttat
    90301 aaacctcgat tgcttgatga ttcccctgat tcactcgcaa aaagatctct ctttcctttg
    90361 tttgcgcagc aagttcttca taccagcgaa aaactgaaga agtcaatcct tgaccacgta
    90421 ctttatttag caaatataat ttacttaaat aaatttttcc atttatctct tcataagcag
    90481 tgtaaccgac agtcttttct tcgcttttta ataaaaagta ggacacacct tctgtcagtt
    90541 ctttttgaat ctgttttttt gactgataag ttttcagcat gtaatccact tgtttttgac
    90601 caatgatcgg cacaaaaact tccggccata cttcttgtat gactgccatc agctcatcca
    90661 gttcattttc tgttactttt attttttcca tacacccacg tcctttttta cattataaat
    90721 gaccttgcgc ttgatggaat aaaaagagca tgatcatgct atacacaccc accgaccacg
    90781 gtttcaagaa aatcacaatc tgcataaatt ccctaaaaga taatttcgtc aaaccagcta
    90841 ctagacagat caagtcgtct ggcgcaaagg gaagcagcaa ggtaacaact agtacttttt
    90901 taatatcttt cgtttttctt gtcagcaatt catcaaattt ctgatacttt tttggcgata
    90961 aaataagctg gacaaaggct acgccatagt aacggacaag caaaaatccg attgcttccc
    91021 caatcactag gccaatacaa ctatataaaa gcccaaatcc attgccaaac atcagcatgc
    91081 ccgctactga ggagatccct cccggcaaaa taggaatgat cacttggatc acttgcaaca
    91141 gaacaaatat catcactgca tacttgccaa attgctggat aaacgcttgc aaagacgtgc
    91201 tcgatcgaaa aatcccatga tgataagcat aaagaatcaa cacaaagaaa aaaaacagac
    91261 caataatggg catgatctga agacattttt gtaaaactga tcctttttta gacatctata
    91321 ttccccacat cttttttcat ttattaaaac tgcctcctta tgatgtgcac cccaaaagtt
    91381 a
//
LOCUS       DACDPS010000004        76578 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.004, whole genome shotgun sequence.
ACCESSION   DACDPS010000004 DACDPS010000000
VERSION     DACDPS010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 76578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.004"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(160..1725)
                     /gene="guaA"
                     /locus_tag="IWJ07_000294"
     CDS             complement(160..1725)
                     /gene="guaA"
                     /locus_tag="IWJ07_000294"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP9331902.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(1784..2707)
                     /locus_tag="IWJ07_000295"
     CDS             complement(1784..2707)
                     /locus_tag="IWJ07_000295"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAP9331903.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            2903..3508
                     /locus_tag="IWJ07_000296"
     CDS             2903..3508
                     /locus_tag="IWJ07_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9331904.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            3816..4832
                     /locus_tag="IWJ07_000297"
     CDS             3816..4832
                     /locus_tag="IWJ07_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9331905.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            4973..6274
                     /locus_tag="IWJ07_000298"
     CDS             4973..6274
                     /locus_tag="IWJ07_000298"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAP9331906.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            6320..6982
                     /gene="deoC"
                     /locus_tag="IWJ07_000299"
     CDS             6320..6982
                     /gene="deoC"
                     /locus_tag="IWJ07_000299"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAP9331907.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            7003..7401
                     /locus_tag="IWJ07_000300"
     CDS             7003..7401
                     /locus_tag="IWJ07_000300"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAP9331908.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            7467..8549
                     /locus_tag="IWJ07_000301"
     CDS             7467..8549
                     /locus_tag="IWJ07_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9331909.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            8761..10323
                     /locus_tag="IWJ07_000302"
     CDS             8761..10323
                     /locus_tag="IWJ07_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9331910.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            10316..11449
                     /locus_tag="IWJ07_000303"
     CDS             10316..11449
                     /locus_tag="IWJ07_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9331911.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            11449..12405
                     /locus_tag="IWJ07_000304"
     CDS             11449..12405
                     /locus_tag="IWJ07_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9331912.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            12504..13670
                     /gene="deoB"
                     /locus_tag="IWJ07_000305"
     CDS             12504..13670
                     /gene="deoB"
                     /locus_tag="IWJ07_000305"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAP9331913.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            13694..14512
                     /locus_tag="IWJ07_000306"
     CDS             13694..14512
                     /locus_tag="IWJ07_000306"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP9331914.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            14529..15236
                     /gene="deoD"
                     /locus_tag="IWJ07_000307"
     CDS             14529..15236
                     /gene="deoD"
                     /locus_tag="IWJ07_000307"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP9331915.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(15534..16220)
                     /gene="gpmA"
                     /locus_tag="IWJ07_000308"
     CDS             complement(15534..16220)
                     /gene="gpmA"
                     /locus_tag="IWJ07_000308"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP9331916.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(16392..16973)
                     /locus_tag="IWJ07_000309"
     CDS             complement(16392..16973)
                     /locus_tag="IWJ07_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9331917.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(17106..17789)
                     /gene="rpiA"
                     /locus_tag="IWJ07_000310"
     CDS             complement(17106..17789)
                     /gene="rpiA"
                     /locus_tag="IWJ07_000310"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAP9331918.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            18108..18521
                     /gene="rpsL"
                     /locus_tag="IWJ07_000311"
     CDS             18108..18521
                     /gene="rpsL"
                     /locus_tag="IWJ07_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAP9331919.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            18605..19075
                     /gene="rpsG"
                     /locus_tag="IWJ07_000312"
     CDS             18605..19075
                     /gene="rpsG"
                     /locus_tag="IWJ07_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAP9331920.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            19169..21256
                     /gene="fusA"
                     /locus_tag="IWJ07_000313"
     CDS             19169..21256
                     /gene="fusA"
                     /locus_tag="IWJ07_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP9331921.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            21428..22615
                     /gene="tuf"
                     /locus_tag="IWJ07_000314"
     CDS             21428..22615
                     /gene="tuf"
                     /locus_tag="IWJ07_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP9331922.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            23022..23330
                     /gene="rpsJ"
                     /locus_tag="IWJ07_000315"
     CDS             23022..23330
                     /gene="rpsJ"
                     /locus_tag="IWJ07_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAP9331923.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            23361..23990
                     /gene="rplC"
                     /locus_tag="IWJ07_000316"
     CDS             23361..23990
                     /gene="rplC"
                     /locus_tag="IWJ07_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAP9331924.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            24018..24641
                     /gene="rplD"
                     /locus_tag="IWJ07_000317"
     CDS             24018..24641
                     /gene="rplD"
                     /locus_tag="IWJ07_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAP9331925.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            24641..24931
                     /gene="rplW"
                     /locus_tag="IWJ07_000318"
     CDS             24641..24931
                     /gene="rplW"
                     /locus_tag="IWJ07_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAP9331926.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            24970..25803
                     /gene="rplB"
                     /locus_tag="IWJ07_000319"
     CDS             24970..25803
                     /gene="rplB"
                     /locus_tag="IWJ07_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAP9331927.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            25846..26124
                     /gene="rpsS"
                     /locus_tag="IWJ07_000320"
     CDS             25846..26124
                     /gene="rpsS"
                     /locus_tag="IWJ07_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAP9331928.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            26147..26494
                     /gene="rplV"
                     /locus_tag="IWJ07_000321"
     CDS             26147..26494
                     /gene="rplV"
                     /locus_tag="IWJ07_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAP9331929.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            26508..27164
                     /gene="rpsC"
                     /locus_tag="IWJ07_000322"
     CDS             26508..27164
                     /gene="rpsC"
                     /locus_tag="IWJ07_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAP9331930.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            27167..27601
                     /gene="rplP"
                     /locus_tag="IWJ07_000323"
     CDS             27167..27601
                     /gene="rplP"
                     /locus_tag="IWJ07_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAP9331931.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            27591..27779
                     /gene="rpmC"
                     /locus_tag="IWJ07_000324"
     CDS             27591..27779
                     /gene="rpmC"
                     /locus_tag="IWJ07_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAP9331932.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            27804..28070
                     /gene="rpsQ"
                     /locus_tag="IWJ07_000325"
     CDS             27804..28070
                     /gene="rpsQ"
                     /locus_tag="IWJ07_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAP9331933.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            28128..28496
                     /gene="rplN"
                     /locus_tag="IWJ07_000326"
     CDS             28128..28496
                     /gene="rplN"
                     /locus_tag="IWJ07_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAP9331934.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            28533..28841
                     /gene="rplX"
                     /locus_tag="IWJ07_000327"
     CDS             28533..28841
                     /gene="rplX"
                     /locus_tag="IWJ07_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAP9331935.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            28869..29408
                     /gene="rplE"
                     /locus_tag="IWJ07_000328"
     CDS             28869..29408
                     /gene="rplE"
                     /locus_tag="IWJ07_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAP9331936.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            29427..29612
                     /locus_tag="IWJ07_000329"
     CDS             29427..29612
                     /locus_tag="IWJ07_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAP9331937.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            29648..30046
                     /gene="rpsH"
                     /locus_tag="IWJ07_000330"
     CDS             29648..30046
                     /gene="rpsH"
                     /locus_tag="IWJ07_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAP9331938.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            30078..30614
                     /gene="rplF"
                     /locus_tag="IWJ07_000331"
     CDS             30078..30614
                     /gene="rplF"
                     /locus_tag="IWJ07_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAP9331939.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            30775..31131
                     /gene="rplR"
                     /locus_tag="IWJ07_000332"
     CDS             30775..31131
                     /gene="rplR"
                     /locus_tag="IWJ07_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAP9331940.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            31152..31652
                     /gene="rpsE"
                     /locus_tag="IWJ07_000333"
     CDS             31152..31652
                     /gene="rpsE"
                     /locus_tag="IWJ07_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAP9331941.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            31667..31846
                     /gene="rpmD"
                     /locus_tag="IWJ07_000334"
     CDS             31667..31846
                     /gene="rpmD"
                     /locus_tag="IWJ07_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAP9331942.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            31890..32330
                     /gene="rplO"
                     /locus_tag="IWJ07_000335"
     CDS             31890..32330
                     /gene="rplO"
                     /locus_tag="IWJ07_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAP9331943.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            32330..33625
                     /gene="secY"
                     /locus_tag="IWJ07_000336"
     CDS             32330..33625
                     /gene="secY"
                     /locus_tag="IWJ07_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAP9331944.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            33687..34334
                     /locus_tag="IWJ07_000337"
     CDS             33687..34334
                     /locus_tag="IWJ07_000337"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAP9331945.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            34525..34743
                     /gene="infA"
                     /locus_tag="IWJ07_000338"
     CDS             34525..34743
                     /gene="infA"
                     /locus_tag="IWJ07_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAP9331946.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            34776..34892
                     /gene="rpmJ"
                     /locus_tag="IWJ07_000339"
     CDS             34776..34892
                     /gene="rpmJ"
                     /locus_tag="IWJ07_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAP9331947.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            34910..35275
                     /gene="rpsM"
                     /locus_tag="IWJ07_000340"
     CDS             34910..35275
                     /gene="rpsM"
                     /locus_tag="IWJ07_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAP9331948.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            35303..35692
                     /gene="rpsK"
                     /locus_tag="IWJ07_000341"
     CDS             35303..35692
                     /gene="rpsK"
                     /locus_tag="IWJ07_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAP9331949.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            35772..36710
                     /locus_tag="IWJ07_000342"
     CDS             35772..36710
                     /locus_tag="IWJ07_000342"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAP9331950.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            36738..37118
                     /gene="rplQ"
                     /locus_tag="IWJ07_000343"
     CDS             36738..37118
                     /gene="rplQ"
                     /locus_tag="IWJ07_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAP9331951.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            37390..38229
                     /locus_tag="IWJ07_000344"
     CDS             37390..38229
                     /locus_tag="IWJ07_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9331952.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            38205..39074
                     /locus_tag="IWJ07_000345"
     CDS             38205..39074
                     /locus_tag="IWJ07_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9331953.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            39064..39861
                     /locus_tag="IWJ07_000346"
     CDS             39064..39861
                     /locus_tag="IWJ07_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP9331954.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            40064..40810
                     /gene="truA"
                     /locus_tag="IWJ07_000347"
     CDS             40064..40810
                     /gene="truA"
                     /locus_tag="IWJ07_000347"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP9331955.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(41265..43934)
                     /locus_tag="IWJ07_000348"
     CDS             complement(41265..43934)
                     /locus_tag="IWJ07_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP9331956.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFFYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(44172..44360)
                     /locus_tag="IWJ07_000349"
     CDS             complement(44172..44360)
                     /locus_tag="IWJ07_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAP9331957.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            44630..44992
                     /locus_tag="IWJ07_000350"
     CDS             44630..44992
                     /locus_tag="IWJ07_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9331958.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            45044..46726
                     /locus_tag="IWJ07_000351"
     CDS             45044..46726
                     /locus_tag="IWJ07_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAP9331959.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            46843..48879
                     /gene="recG"
                     /locus_tag="IWJ07_000352"
     CDS             46843..48879
                     /gene="recG"
                     /locus_tag="IWJ07_000352"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAP9331960.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            48928..49929
                     /gene="plsX"
                     /locus_tag="IWJ07_000353"
     CDS             48928..49929
                     /gene="plsX"
                     /locus_tag="IWJ07_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAP9331961.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            50051..50293
                     /gene="acpP"
                     /locus_tag="IWJ07_000354"
     CDS             50051..50293
                     /gene="acpP"
                     /locus_tag="IWJ07_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP9331962.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            50561..51565
                     /locus_tag="IWJ07_000355"
     CDS             50561..51565
                     /locus_tag="IWJ07_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9331963.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            51566..52510
                     /locus_tag="IWJ07_000356"
     CDS             51566..52510
                     /locus_tag="IWJ07_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9331964.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            52510..53472
                     /locus_tag="IWJ07_000357"
     CDS             52510..53472
                     /locus_tag="IWJ07_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9331965.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            53499..54413
                     /locus_tag="IWJ07_000358"
     CDS             53499..54413
                     /locus_tag="IWJ07_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9331966.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            54439..56220
                     /locus_tag="IWJ07_000359"
     CDS             54439..56220
                     /locus_tag="IWJ07_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9331967.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            56568..57254
                     /locus_tag="IWJ07_000360"
     CDS             56568..57254
                     /locus_tag="IWJ07_000360"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP9331968.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            57274..60855
                     /gene="smc"
                     /locus_tag="IWJ07_000361"
     CDS             57274..60855
                     /gene="smc"
                     /locus_tag="IWJ07_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAP9331969.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            60864..61673
                     /locus_tag="IWJ07_000362"
     CDS             60864..61673
                     /locus_tag="IWJ07_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9331970.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            61684..62682
                     /gene="ftsY"
                     /locus_tag="IWJ07_000363"
     CDS             61684..62682
                     /gene="ftsY"
                     /locus_tag="IWJ07_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAP9331971.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            62869..65136
                     /gene="gshAB"
                     /locus_tag="IWJ07_000364"
     CDS             62869..65136
                     /gene="gshAB"
                     /locus_tag="IWJ07_000364"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAP9331972.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(65225..65677)
                     /locus_tag="IWJ07_000365"
     CDS             complement(65225..65677)
                     /locus_tag="IWJ07_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAP9331973.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            65827..66777
                     /locus_tag="IWJ07_000366"
     CDS             65827..66777
                     /locus_tag="IWJ07_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP9331974.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            66770..67729
                     /locus_tag="IWJ07_000367"
     CDS             66770..67729
                     /locus_tag="IWJ07_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP9331975.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            67726..68481
                     /locus_tag="IWJ07_000368"
     CDS             67726..68481
                     /locus_tag="IWJ07_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9331976.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            68504..69457
                     /locus_tag="IWJ07_000369"
     CDS             68504..69457
                     /locus_tag="IWJ07_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9331977.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(70101..70397)
                     /locus_tag="IWJ07_000370"
     CDS             complement(70101..70397)
                     /locus_tag="IWJ07_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAP9331978.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            70752..71108
                     /locus_tag="IWJ07_000371"
     CDS             70752..71108
                     /locus_tag="IWJ07_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP9331979.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            71118..72017
                     /locus_tag="IWJ07_000372"
     CDS             71118..72017
                     /locus_tag="IWJ07_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAP9331980.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            72534..73265
                     /locus_tag="IWJ07_000373"
     CDS             72534..73265
                     /locus_tag="IWJ07_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP9331981.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            73410..75437
                     /locus_tag="IWJ07_000374"
     CDS             73410..75437
                     /locus_tag="IWJ07_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAP9331982.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            75545..>76578
                     /locus_tag="IWJ07_000375"
     CDS             75545..>76578
                     /locus_tag="IWJ07_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP9331983.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLF"
BASE COUNT        24967 a        13833 c        16542 g        21236 t
ORIGIN      
        1 gtagtgaata aaagcaaagc ccttgttagt agttaatcta acaagggctt tggtagggaa
       61 aatggtagga aaccatctta ttttttacga aaatcaggaa ctttagtaat tgaataatgt
      121 tgatttatag gtattcctca tccatattat gttggctctt tattcccact ccactgttgc
      181 tggtggttta gaagtgatgt catacacgat acggttgacg tgggctactt cgtttacgat
      241 acgtacagag attttttgca agacttccca tgggatacgg gcaaagtcag cggtcattcc
      301 gtcgattgaa gttacagcac ggataccgac tgtgtagtcg taagtacgtc cgtcacccat
      361 aacaccgaca gagcggatcc ctggaagaac agtgaaatat tgccagatat cacggtcaag
      421 tccagcagct gcgatttctt cacgcaagat agcatcagat tcgcgtacga tttctagctt
      481 gtcttcagtg atttcgccaa gaacacgaat accaagacca ggaccaggga atggctgacg
      541 ccaaacgatt gaatcaggca tgcctaactg agtccctaat tcacggactt cgtctttgaa
      601 taatgtgttt aatggttcga tcaattcaaa ttgcatatct tctggcagtc cacctacatt
      661 atggtgagat ttgatcgtct gtgctgtttc tgtaccagat tcgataacgt cagtatacaa
      721 cgttccttgt gctaggaaag agacgccttc ttcaccagca agttttgttg cttcgtcatc
      781 aaaaacatat acgaattcat tcccaatgat tttacgtttt tgttctgggt cagatacacc
      841 tttcaacttg tctaagaagc gatctcttgc attgactttg atgatgttca agccgaattt
      901 tccgcctaga ctttccatta cttgttccgc ttcgcctttg cgtagcagac cgtggtcaac
      961 aaagatagat gtcaattggt caccgatcgc tttttgtaaa agaaccccaa cgacactaga
     1021 atcaacgcca cctgaaagac caagcaagac ttttttgtca ccaacttgtt cacggatttt
     1081 ttctacttgc atatcgataa agttatccat tgtccagtta ccttcacagt gacaaacatc
     1141 aaaggcaaag tggcgcagca attcgattcc gtattcggaa tgacgtactt ctgcatggaa
     1201 ttggatacca taaaaattac gtgttgtgtc ttggatagaa gcaatcggac aatctttgct
     1261 tgttccgact gtttcaaagc cttcaggaac tttagttacc aagtcaccat ggctcatcca
     1321 tactgtttgt tgttttggtg tttctttaaa caagacagct tgatcgcttg ttacttctaa
     1381 caaggctttc ccatattcgc ggtttttagc aggctctacg gaaccgccaa gtttgtacgt
     1441 gatcaattgc ataccatagc aaatccctaa tacaggaata cctaaattga agatctcagg
     1501 atcgatccca aatgcattct catcatatac actgtttggt ccgccagaaa gaatgattcc
     1561 ctttggattc atcgctttta cttcttctgc agtaatgcgg tggctcaata attcggaaaa
     1621 gactccaaga tcacgaatac gtcttgtgat caattggtta tactgactac cgtagtctaa
     1681 tacgataatc ttttcaacgt tcgtcatatc ggcaacgttt gtcaaattta ttacccctat
     1741 ccctttaaat tttatctcaa aatagtcaaa ctattttaaa atgctaatat tttcgcatga
     1801 aaatcgaatc gatgatatga ttttcagttt tatggagaat tatatcggct cttcctcgtg
     1861 taggcaagat atattcctgt aaatttttca aattcacatt tttccataca tcttttgcca
     1921 ttttaaaggc ttcttcacga tctccgatag caaaacgata ataataattc tctggatttt
     1981 ggaaagccgt atccagtaac gcaccaaaac gatccaaata ccatttttcg atcagcacgg
     2041 gatcggcatc gacatagaca gaaaaatcaa aaaagtcgct gacgtagatt tgctgattcg
     2101 caggtaattg aagcgtattg atcccctcaa cgatcaagat atcaggctgc tggatcaatt
     2161 cgtacttgtc tggaatgata tcatacacat catgtgagta aacaggtact tttatctctt
     2221 cctgaccaga ttttacacgg ttcaagaaat cgatcagcat ctccatatca taactttcag
     2281 gaaatccttt acggtccatg attccacgct cttttaatat acgatttgga taaagaaatc
     2341 cgtcagtcgt gatcagctga acgtttcttc gtttgaaggt acgagacaaa atcgtctgca
     2401 gcagtctggc tgttgtactt tttccaacgg ctacacttcc ggcgatcccg ataataaaag
     2461 gcggtacttc tacgtattta tgaagaaata aacctttact caagaacaaa gattcaaatt
     2521 ctttcatata aagataaatc agatgagcaa gaggtacata gatatctcga acatcctgca
     2581 tggaaatgcg gtcattcaaa cttttgatat tatctagttc atcttgtgtc aaaggtgctt
     2641 gcccattacg ataaaattct cgccattctt ctcttgccac acggtagtag ttcattggat
     2701 cattcatatt agcctcctgc gttctcccat caatggaact attttaacac aaaataaacg
     2761 caaaccccac cacttaagag agattgccga tctttttgta taatctttat aatagaaaga
     2821 aacggagtct tattcttgac ttgcgcacgg atacataaaa ggatagactg ttgtcatgaa
     2881 agaaatgaga aggatggaag acatgacaaa tcattactat tctgaacgcc cagatacggc
     2941 gcacgatttt gaacaatggg cttttgagtt aaaaggcaga acctttcaat ttgtaacaga
     3001 ttctggtgta ttttcacgtg aaacagtgga ttatggttca cgtgtcctga ttgatgcttt
     3061 cgattgggaa gagttacctg atgaaggaaa gatcttagat gtgggatgcg ggtatggacc
     3121 gattgggtta gctttggcat ttgcgacaca gcggtttgtt gaaatggtcg acattaatga
     3181 acgagcggta agtctagcgc aaggaaacgc aaagcgtaac caaatcgaac atgttgatat
     3241 ccatcaatcg aatatctatg aagcagttca tgaagaaacc tatgcagcta tcgtaagcaa
     3301 tccgccgatc cgtgctggaa aaaaagttgt acatgaaatt ttgacaggcg catatcctcg
     3361 gttgaagaag ggcggtacac tgacaattgt gatccagaag aaacaaggag cgccgagtgc
     3421 gcaaaagaaa atggaagaaa catttggtaa tgcagaaatc gtcacaaaag ataaaggata
     3481 ttatatcatc aaaagtgtga aagaataact agctgaaaaa tagtggagcg acgaaagagt
     3541 ggaactgggc gtgcatgtac gggtacgtat cagttctttt tttgttgaga agtttttaaa
     3601 aacaaagaac tattttttct tcaaaaagaa atagtaggaa cagagtcgtt gttttttacg
     3661 ataaaataaa taagatacag aacttgtata gaaggctgcc aagtcggtag ataaaggagg
     3721 tttttatcaa aaaacatact ttcctttttt ctctattctt ttcttggggg ttttggtaca
     3781 ctgataggga atttagatag aagggagcca ataggatgaa gctgaccatc agagatatcg
     3841 cagaaatggc aggcgtatca gtaacaactg tctcacagat tttgaataac aaaggaagca
     3901 gatttagtga taagacaaga aaaaaagtgt tgtcgatcgt gaatgaacat cattataagc
     3961 ccgattattt cgcttcaaat gtgatcaatc gacattcaaa gaccatcgga atgatcgtcc
     4021 ctgatgtcac tgatttcttc ttctccaaac tgatcgaagg tgtagagagc tatttgaatc
     4081 ctttgggcta tgtgatcatg ttatgcaatt ctcgtcatag ccaagaaaat gagatcaagt
     4141 atttacaaga gctttcccac cgctcagtgg acggaatctt gctggccaca ccaaatattt
     4201 tacctgagaa atatgcatta gacagcggtt tttatcaaaa aatgccaatc attttaatcg
     4261 atcgcggatt gaatcgtcgg gataacggac ggcttatcgt aaaggaatat gagggcgcct
     4321 atcaagctgt ttcgctgttt attgaaaatg gccatacgaa gatcggcatg ctgaaggaaa
     4381 ccactggata ctatcaatta gaagaacgtt tcaacggata tcgtcatgcg ctgaaagatc
     4441 atggcctgcc ttttaatggg aaatttgttg aacatggaga gttgactgtc caaggtggat
     4501 atactgcatc aaaaaaatta ctcaaacata aagatatcac ggctattttt tgcgggaatg
     4561 atgccatggc gatcggatgc tatcaggcga tcgatgaatt gggcaaaaaa atcccagaag
     4621 atatctcagt cataggattc gatggattaa agctgtcaga atacatgatt ccgcaattga
     4681 cgacagtgca gcagccaagt tttgatattg gattttatgc tgcacgtttc ctgatcgata
     4741 cgatcgaatt tcctcaaaga aaagttccga acaaagtgtt cgaaacaaaa ttaattattc
     4801 gtgagagcgt aaaagtattg acaaagggct aactcggcgg tatattgctt atgtaagcat
     4861 ttacaaaggc tagttagtgc tttttttatt tttagtaaac cagtttacta aagtggttta
     4921 cctttcctaa aaataatgta taataccgaa tgaagacaag gaggatcagg caatgcgaat
     4981 ggtggatctg attgaaaaaa agagggatgg aaacgagctg tcgaaagagg aaatcgagta
     5041 catcgtcacg aactatacga atggaaagat tccagattat caagtcagtg cattattgat
     5101 ggcaattttt tatcaagaca tgacaaatga agaaatcacg aacttaacat tggcgattgc
     5161 taattctggt gatgtcatcg atttgagttc tcttgaagga ataaaggtag ataagcattc
     5221 gacaggtggc gttggtgaca cgaccacatt gattttagcc cccctagtag ctagtgtcgg
     5281 agtgacagtg gctaaaatgt ctggacgcgg tctaggttac acaggtggta cactagacaa
     5341 gctagaagca attcccggat ttcagatcga gctttcggat gaagaatttg tacgcattgt
     5401 taatgaaagt aaagtagcgg taattggtca atcaggaaac ttggcaccgg cggacaaaaa
     5461 attgtatgcc ttgcgtgacg tcacagcgac cgttgattct cttccgctga ttgccagttc
     5521 gattatgagt aaaaaaatcg cggcaggagc agatgcaatc gttttggatg tgaccactgg
     5581 agacggtgct ttcatgaaga acatcgaaga tgcaagacgt ctggcaaaga ccatgacgag
     5641 cattgggaaa ctggctaacc gagaaacagt ggcggttatc tcagatatgt cagaacctct
     5701 tggcgaagct atcggaaata gtctagaagt tgtcgaggca atcgaaacat tacaaggaaa
     5761 tggtccagaa gatctagtag aaatgtgcta cgccttagga agccaaatgg ttgttcttgc
     5821 tggcaaagct aagacaaccg atgaagcaag aacgttgctt caagaagctc tggaatcagg
     5881 taaagcgtta gcaaaattca aagaaatgat ccaaaatcaa gggggagacc cagcgatcgt
     5941 ggaacatcca gaacgaattt tgactgcacg ctacacaatg gagcttcctg ctaaacaaag
     6001 cggagttgtg tcaaaaatcg tggcaaatga attaggaatt gctgcaatga tgctaggtgc
     6061 tgggcgtaaa accaaagaag aagatattga tcacgctgtt ggattgaaac tgcataaaaa
     6121 aattggtgac actgtgacaa aaggtgagtc actattgacg atctatagta acgacgaaga
     6181 aatcacttct gtcattgaat tattatataa aaatattgaa attggtgaat cggcaatgaa
     6241 gccaacgttg attcatgaca tcatcacaga ataaaggaaa aaagcggact ttaccgtttt
     6301 aaagaaaagg ggaaaagaaa tggaactaaa ccgaatgatc gaccatacta ttttgaaagc
     6361 agacgcatct aaagaagctg tgatgcaaat tatcgaagaa gcaaaaaaat accattttta
     6421 ttctgtctgc atcaatccag catgggttgc gctagcgaaa gaacaattac aaggtgagcc
     6481 agtagccgta tgtacagtaa tcgggtttcc tctaggagca aacacacccg agacaaaagc
     6541 atttgaaaca acagatgcca tcaataacgg tgcagatgaa gtcgatatgg tcatcaatat
     6601 cggtgctttg aaatcaaagg atgatcaaca agtccaaaaa gatatcgaag cagttgttga
     6661 agcagcaaaa gatcgtgcgc ttgtcaaagt tattattgaa acatcgttat tagatagaga
     6721 agaaatcatc cgagcttgcg aaatagcaaa agctgcaggc gctgattttg tcaaaacatc
     6781 aactggtttc tcaacaggcg gagcaaaggt agaggatgtc cgtttgatgc gtgaaactgt
     6841 aggaccagaa atgggtgtca aagcttccgg cggtatccat aacgaagaag aagcaatggc
     6901 aatgatcgaa gctggtgcta caagaatcgg aacaagcgca ggagtagcaa tcgtatctgg
     6961 ttcaactggt gaaggctatt aattttttca aaggagaagc tcatggcaga actcaaacaa
     7021 gaatggatca acacagctat tgaagcgtta gataaagcct atgttcctta ttcccatttt
     7081 ccggtaggcg cttgtctcgt aacggaatca ggcaagattt atcaaggcat caacattgag
     7141 aatgcatcat ttggattaac gaattgtgcc gagcgcacag cttttttcaa agcagtatct
     7201 gaaggagaaa aaacatttac gcatctagtc gtagcggggc acacccccga tcctatctct
     7261 ccttgtggtg catgtcgaca agtaatggca gaattctgtt cccctgatat gccggttacg
     7321 ctagtaggag acaatggtgt gacgaaggca acgaccgttc gtgcactgct gccttatgca
     7381 tttactgaaa aagatctata actcatgttc attcagtcag ctgactgttg aaaaataaaa
     7441 caattatttt aggaggcttt acagaaatga aaaaagcaaa attatttggt ttaggtgctg
     7501 tcgcactagc agcagggtta tttttaggtg catgcggcaa caacggctct actgattcat
     7561 ccggtggcaa aagctcatca gacacaacaa ctgcagcatt gatcacagac acaggtggtg
     7621 tagacgaccg ttcattcaac cagtcagctt gggaaggact agaaaaatgg gggaaagatc
     7681 aaggactttc acgaggaaat gatggcttcc aatatttcca atcttcaaat gaatcagatt
     7741 acatcccaaa tattgaccaa gcattgaatg ctggattcaa aacgattttt ggtattggtt
     7801 ataaactaaa accagcgatc caagaacaag caacaaacaa tacaggtacg aactttgtta
     7861 ttatcgatga tgtcatcgat ggattagata acgttgtatc tgcaactttc aaagacaacg
     7921 aagcttctta tcttgcaggt gttgcagcag cttatacgac tgaaacaaac gtagtcggct
     7981 ttatcggcgg tgtaaaaggt gaagtaatcg atcgttttga tgctggattc aaagccggtg
     8041 ttgatgcagg tgcgaaagaa ttaggaaaag aaatcaaagt attgaatcaa tatgctggtg
     8101 acttctctgc tccagataaa ggacgttcaa ttgctcaagg tatgtatgca caaaacgcag
     8161 atatcatctt ccatgcttca ggcggtacag gtaacggtgt attccaagaa gcaaaatcat
     8221 tgaacgaatc aggcgacaaa aaagtttggg ttatcggtgt tgaccgcgac caatcagacg
     8281 aaggcgaata cacattaaac ggcgaaaaga aaaacttcac attgacatct acactgaaag
     8341 cagttggaac agtagtagaa gatttggcac aaaaatcagc ggacggtaaa ttccctggcg
     8401 gcgaacacac tgtttatgga ttgaaagaag acggtgttgg tctaacagaa ggacaacttt
     8461 ctgacgaagc gaaaaaagca gttgatgaag caaaagaaaa aatcatttca ggtgacgtaa
     8521 aagttccaga aactcctgaa gaaaactaaa accatttcta tagccttgtt agacacatgt
     8581 ttgacaaggc tatggtttga ttagaacaag aattgttcat ttatccaagc tgccttgtga
     8641 cagtttggat aagggaacaa ctccctgaag taatgtttgt ttcaaaagaa gaacgctcac
     8701 taagcgtgaa tcttagtcaa tcgttgccct agatagaaat cgttaggatg tgaaaaaaag
     8761 gtggttcaag aaaatattgt catagaaatg cagaatatta ccaaagaatt tggaacattc
     8821 aaggcaaatg acaacatcaa tttacaagta aaggcaggag aaattcatgc gttgcttgga
     8881 gaaaacggtg ctggcaaatc tacattgatg aacgtgcttt ccggattatt agagccgaca
     8941 tcagggaaaa ttttgatgcg tgggaaagaa gtaaagatca caagtccgac caaagccaac
     9001 caattaggga ttgggatggt ccatcagcac tttatgcttg ttgatgcctt tactgtaaca
     9061 gaaaacatcg tgttgggaag cgagcctagt cgcgcaggga tgcttgacca taaaaaagca
     9121 cgaaaagaga tccaaaaagt ttctgaacag tatggattgt cagtcaaccc agatgcttac
     9181 gttcgtgata tttcagttgg aatggaacaa cgggtagaaa ttttaaaaac actttaccga
     9241 ggagcagatg tactgatttt tgatgagccg acagctgtat tgacccctca agaaattgat
     9301 gaattaatcg tgatcatgaa ggaattagtc aaagaaggca agtcgatcat tttgattacg
     9361 cataagttag atgaaatcaa agcagtagct gaccgctgta cagttatccg ccgtggaaaa
     9421 ggaatcggta cagtcaacgt taaagacgta acctcacaac aattagccga tatgatggtt
     9481 ggaagaacgg tttcattcaa aacgatgaaa aaagaagcga agcctcaaga agtcgttttg
     9541 tctattgaaa atctagtggt gaaagaaaat cgtggattag aagccgtgaa aaacttgaac
     9601 ttagaggttc gtgctggcga agtacttggt atcgctggaa tcgatggaaa cgggcagtcg
     9661 gagttgatcc aagctttgac tggtttgcga aaggcagaaa gcggacatat caagctaaaa
     9721 ggggaagaca tcactaataa aaaaccccga aaaatcactg aacatggtgt aggacacgtg
     9781 ccagaagacc gtcataaata cgggttagtc ctagatatga cattatctga aaatatcgca
     9841 ctacaaacct atcatcaaaa accttacagt aaaaacggca tgctgaatta ttcagtaatg
     9901 aatgaacatg ccagagaatt gatcgaagaa tatgatgttc gaacaacgaa tgaacttgtt
     9961 cccgcaaaag ctttatcagg cggaaatcag caaaaagcaa ttatcgctcg tgaagtcgac
    10021 cgagatcctg atctgttgat tgttgcaaat ccaactcgtg gactggatgt aggtgcgatc
    10081 gaatttattc ataaacgtct gatcgaacaa agggacaaat acaaagcagt gttactgatc
    10141 agtttcgaat tagaagaaat tttaaatgtt tcggatcgta ttgctgttat ccatgaagga
    10201 gaaattgtcg gtatcgttga tccgaaagaa acatctgaaa atgaattagg tctattgatg
    10261 gcgggctata ccttagaaga agcacgaaaa gagttagaaa agggggctgg cgcacatgaa
    10321 tgatcgcagc gaaaaattcc gtaatttatt agtgcctgta ctttcagttg ttttaggatt
    10381 gatcttaggc gcgatattga tggctgcttt tggttttcag cccatccaag gttattcttc
    10441 tatgctagat gcagcccttg gttcgcaaag agggataggt gaaacgttaa ggcaagcaac
    10501 gccgttgatt tttacagcat taggtttttc tgtagctaac tcagctgggt ttttcaatat
    10561 tgggctttct ggacaagcac tttgcgggtg gatcgtcagt gtctgggtgg cacttgcgtt
    10621 tccagatctg ccaaaagttg ttttgcttcc gttatgcgtg atcttaggtg ccttagctgg
    10681 tgcgatggca gcggctgttc caggtttact tcgagcattt tttggaacaa gtgaagtgat
    10741 cgtaacaatc atgatgaact atatcttatt atacgtaagc acatttgctt tacatgaagt
    10801 gatgcctgaa agctatcgct caagtttgga ctcttccaat atgatcagtc aaaatgcctc
    10861 actgcggtta ccatggttaa cacaagcttt tggcggctct cgcgtaaact caggattatt
    10921 tttagcaatc atcgcgctta ttttcgtttg gctgctgatg aagaaaacga ctttaggatt
    10981 tgaaatccgt tctgttggat tgaatccttt tgcttctgag tatgcaggaa tgagtagtaa
    11041 acgtacgatc gttttgtcga tggtcatttc tggcgctttg gcagggcttg gcggtgtggt
    11101 tgaaggatta ggaacgtatc aaaacttctt tgttcaaacg acttcgctat ctattggatt
    11161 tgacggaatg gctgtttcct tgcttggttc tggatcagca atcggtattc ttttatcagc
    11221 gatcttgttc agtattttga agatcggtgg acttggcatg cagacaggag caggtgttcc
    11281 gtttgagatc gtcaatgtat cgattgcttt gattatcttc tttgtcggga tcaacttcct
    11341 gattcgtttc gtcatggcta aagtcttcca agggaaaaaa caagaagaga tcgttgcgac
    11401 aatagaatcg aaaccaacga atcaaaacca tgaagggggc gaactataat gtcgactatc
    11461 gaattaattg ccttgatcct tacttctaca ctggtttatt caacaccgct aattctaacg
    11521 tcactaggcg gaactttttc tgaacgaagc gggatcgtaa acgtagggct agagggaatc
    11581 atggtcatgg gtgcttttag ttccatcgtt ttcaacttga cgatgagtcc atcacttggt
    11641 tctatgacac catggatcgc tattttagtg ggtggcgtcg taggtatcct attttctctg
    11701 attcacgcag tagcaacagt cagtttgcgt gctgaccata ttatcagcgg gacagtaatt
    11761 aatctgatgg cacctgcttt aggtgttttc ttgatcaaag catggtatgg taaaggtcaa
    11821 actgataaca tcacgcagaa tctggggtat ttttctttcc caggattagc agatattccg
    11881 gtcattggac caattttctt taaaaataca ttcttacctg cttattgtgc tattttgatt
    11941 gctgtcctag cttgggtgat catcttcaag acgaaattcg gcttgcgcct ccgttcagtt
    12001 ggtgaaaatc ctcaagctgc cgacacatta ggtatcaatg tatatgctat gcgttattca
    12061 ggcgtactga tttctggctt gttaggcggt atgggcggtg ctgtttttgc tcagtcgatt
    12121 tccggtaact tttcagctgg tacgatcgtt ggacaagggt tcatctccat ggctgctatg
    12181 atctttggta agtggaatcc tttaggggca atgggtgcct cattgttctt tggttttgct
    12241 caaagcttga gtatcattgg cgcacaatta ccagttattt cttcgattcc gccagtattg
    12301 ctgcaaattg ctccatattt gctgacaatc attgtgctgg ttgttttctt agggaaagct
    12361 tctggtccaa aagcaaacgg gaagaactat atcaaatcaa aataatcttt tagcgaataa
    12421 aaagggtggg ttacgggaag tgtttttcgt ctcgcccttt ttgttttaaa ggcaataaaa
    12481 ataaaaagaa agaaggaata atcatgttta aacgcgtaca tctgatcgtc atggattcag
    12541 taggaattgg agaagcacca gatgccgata agttcggtga tgttggttct gatacattag
    12601 gacacattgc gaaagaagca ggattgaacg ttccacattt agagcagctt ggtttaggaa
    12661 cgatccgtcc tcttgaaggt gtggaatcag tgaaagatca tcaaggctat gcgacgaaat
    12721 tagaagaagt ttctgtaggg aaagatacga tgacaggcca ctgggaaatc atgggattga
    12781 atatcaaaac tccatttcgt gtgtttccta atggattccc tgatgatctg atcaaacaaa
    12841 tcgaagaatt ttctggccgt aaagtcgtat gtaacaaacc ttatagcgga acagctgtta
    12901 ttgacgatta cggtgaacac caaatgaaaa caggcgattt aattgtatat acttctgccg
    12961 atcctgtcct tcagatcgcg gcacatgaag aaatcattcc tttagaagaa ttatatcgga
    13021 tttgccaatt cgttcgtgat ataacaaaag atgatccgta tatgattggc cgaatcattg
    13081 cacgtccgta cgtgggtgaa ccaggagact tcacacgaac aagcaatcgc catgattatg
    13141 cgttgaatcc attcggacac acagtccttg attctttgaa agaagcagga aaggacgtga
    13201 ttgctgttgg aaagatcaac gatattttca acggacaagg aatcactgaa tctgtccgca
    13261 cgaaaagcaa tatggatggc gttgaccagc tgctgaaagt tatgaaaaac gatttttcag
    13321 gaatcagctt taccaattta gtagattttg atgcattata cggtcatcgt cgggatgtag
    13381 tcggctatgc gcatgcgatt gaagaatttg acttacgaat tccagaattg cttgaagcga
    13441 tggaagaaga cgacttgcta atgattacag ccgatcacgg aaacgatccg acgtttactg
    13501 gtaccgatca tacgcgagaa tatgtaccgt tattggttta cagtaaaaaa atgaaagaac
    13561 aaggggcatt gcctcaagga tattattcag atatttcagc aacgattgct gagaacttta
    13621 gtgtttcttc gactgaaaat ggcgaaagct tcttgaaatt attaaaataa tctttttaaa
    13681 taggagaaag caaatgacca agttaagcga gatgttacaa gaaacgaccc agtttatcct
    13741 tgataaagga gtaaaagaaa ttgattttgg tttgattcta ggttcaggat taggtgaatt
    13801 agcagatgaa atcgaagatg ctgtaatgat ttcttatgat gagatcccgt tctttcctgt
    13861 ttcgacagta gttgggcatg caggaaagct agtttatggg tctctggcag gcaagaaagt
    13921 gttagccatg caagggagat tccactacta tgaaggtcat tccatgcaaa cagtgaccta
    13981 tccagtacgt gtgatggccg gactaggggc ccattcagtc attgtgacta atgcatgtgg
    14041 tggtgtcaac gaatcatttg taccaggaga tttgatgctg attaccgatc atatcaattt
    14101 tactggtgaa aatcctttga tcggtccgaa tgaagatgca atgggcccta gatttccaga
    14161 tatgagtcaa gcttatacgc ctgactatca agcaacagca aaagcagcag cagaaaaact
    14221 aggtttggat cttaaagaag gcgtatatat gggctattct ggaccaactt atgaaacgcc
    14281 tgcggagatc cgaatgtctc ggacaatagg agccgatgca gtagggatgt cgactgttcc
    14341 agaagtcatc gtagcagctc acagcggatt aaaagtgtta ggaatctctt gtattaccaa
    14401 cttagcagca ggtatgcaag cgaatctgag ccatgaagaa gttgtagaaa caactcagag
    14461 agtcaaacaa tcttttaaag cattggttaa agaaacattg acattattgt aataggagga
    14521 acaataaaat gagtgtgcat attgaagcga aagaaggcga gatcgccgat aagattttac
    14581 ttccgggaga tcctttgcga gctaaatata ttgcggagac ttttttggaa gatccagttt
    14641 gttacaatca ggtacgcggt atgttaggct ttaccggcaa atataaagga gaacgtattt
    14701 ctgttcaagg aacaggaatg ggaatgccat cagccaccat ctatgcccat gaattgatcc
    14761 agtcgtatgg tgtaaagaaa ttgattcgag taggtacatg cggtgcattg tcaaaagacg
    14821 tccatgttcg tgatttagta ctagcacaag gtgcagcgac cagttcttcg atgatcgaaa
    14881 aaaatttcca agcctttcat tttcctccaa tcagtgattt caatctatta ctgaaagcat
    14941 atgagatagc taaagaaaag ggctatacgg ttcatgtagg aaatgttctt tcggaagatt
    15001 ctttctacaa agatgatttg acagagacgt tccaattagc tgaacttggc gttttaggag
    15061 tggaaatgga agcagcggct ctttattact taggcgcgaa ataccatgtg cagacattaa
    15121 gtttgatgac cgttagcgac catttgatca ccggtgaaga aacaacagct gcagaacgtc
    15181 aatctacgtt caatgaaatg atcgaagtag gattggaaac agcgatagca gaataagaag
    15241 gaaatgaaaa agctgccctg cattaagtaa taagaacatc caagcaaaaa tagctcctca
    15301 tgtttttgac ttggctggac ttattattgc aaatgtaagc ttttgaacac cgtttaccaa
    15361 gaggttgtga atctgccgtt taactctgag tcacaaggaa caatttcaaa aaacagcttt
    15421 tcatgtttga tgaatattgt gacttgtgac cgagaagttg gcaggagaaa accgtttata
    15481 cgaaaaaagg gactgtgaca aatcgtgtca cagtcccttt tttatagtgt tttttatagg
    15541 tagtattttt tagcaactgt taagtcatca tttaattcat aaacaagtgg ttgacctgtt
    15601 ggaatttcaa gatccatgat gtcttcgtca gagatacctt cgatatgttt agctagggcg
    15661 cgtaatgagt taccatgtgc agctactaaa acagttttgt tgtctagtaa tgctggagca
    15721 atttcatctt gccagaatgg caaagcgcgt tctaatgtca ctttcaagtt ttctccacca
    15781 ggaatatcgc gtttgtctaa cattgcgtaa cgacggtcat ttgctgcaga accttcgtca
    15841 gtttcttcca ttaatggagg aagagtatcg taagaacgac gccagatgtg cacttgttcg
    15901 tcgccgtatt tttcagctgt ttcttttttg ttcaaacctt gtaatttacc atagtgacgt
    15961 tcgtttaagc gccatgattt gatttgtggt acccaaagtt gatctgaata ttctaagatc
    16021 aagttacatg ttttgattgc acgagttaaa acagaagtgt aagcaacatc aaattcgatt
    16081 ccagcttctt tgattttgcg tccgccttct tttgcttctt cgattccttc tggtgctaga
    16141 tttacatcag cccaaccagt aaattggttc aatgcattcc attcactaag accgtgacga
    16201 gaaaaaacta attttggcat gtttagctct ccttttaaga taggtttata tttctgaaat
    16261 tttcagttct gttttattgt acaatttttt ggaagagatt tccatcgaaa aattagataa
    16321 gattctatga gaaaacaagt agagaatata aaaaaacgaa cagtgcccac gcaactgttc
    16381 gtttttaggt attagatgca taattgctcg atttcttttt caggtaaatt gatatattta
    16441 taacatgttc caactgaaac accgcatttt gaagaaataa attggatcgt ttcttttttt
    16501 tcataataga gacgtcggat ttttttgatc gtttttgcat cgatttttgg tcttccgccg
    16561 atttttcctt ttttacgagc ttcatctaat ccaactaatg tacgttcttt gatcaatgcg
    16621 cactccatgt ttgctaggtt ttccattaat tggaaataga tttttcccat ttcgcgcgta
    16681 tcgattcctt catcaagact aatcaagtgc agcccgcgat ctttgaataa ttgcgtgagt
    16741 tctgttaatt gccgcgtaga tttgcctaat cgatgaagtt ggcaaatgac tagtgcatcg
    16801 ccttgttgca tgtcagcgat cactgactcc aaatgaacag cagtagaatc attttgttcc
    16861 acttcatatg tctcatggaa aatttcttga caaccaaagt cagataattg atcaacctga
    16921 gttgttaaat caacgtcatt tatggttgtg cgtgcataac caataatttt catggtgatt
    16981 cctttctagc agtgattttc taactggata taacagtaaa aatgtagcat aaagtacgtc
    17041 aaaaagatag ttttttcgca aattttccgc tgtctttttt tattggatga tcaagcagtt
    17101 tttctttatt tacgacggat tgcgtcaaga atctcaacgc ctttttctga gccgacaact
    17161 actttagaaa ccatgtttag gaaaagccca tgttctacta ccccaacaag ttcatctaaa
    17221 taggtagcta agctttctgg ctgttcgatg acttctagat gcagatcaat gatgtaatga
    17281 ccaccatctg tggttaagac ttctccgttt tcgtctgtac gcaagacggg ttggaatctt
    17341 ttttcttcaa aaatatgaag tagctgttgg gagccataag ggatcacttc aactgggagt
    17401 gggaacttcc caagtttatc gactaattta gaagaatcaa cgatccaaat cgtttctttt
    17461 gagtacgtag cgactatttt ctcgaaaagc aaagcagcgc cgccgccttt gatcccttga
    17521 aaatcagcac tgatttcatc agcaccgtct attgtcaaat cgactacggg gacttcatca
    17581 atactcttta acggtattcc taatttttcc gcttgttctt ttgtcgcatt tgaagtagta
    17641 actcctgtga tacgtaaacc ttcttcacgc atacggcgac caatctcttc taccatataa
    17701 taggcggttg aaccggtccc gagacctacg atcatcccgt cctttacgaa tttcgcggct
    17761 tcaatgccag ctaattgttt taaattcatc tttgcgtcca ccttttttta gtttgagcat
    17821 cgtgctttcc tcactatttt agcgtgattt acggtaaaaa gataccattc ttttttattc
    17881 caataaagct ttttcaaaaa aaattgcatc tcttgttgac atgttttttc attcgtgata
    17941 ttctaacaaa ggtgctttat ctacaggttt ctgtgaagaa acgtgctgac tgtttatcaa
    18001 agcatatttg ataaggatgc agacattgca tttatttttt atcacaaaaa aagaaccacc
    18061 tggatgtgtg gaactagacg caaatcgtgg gaaggaggaa acaaggaatg cctacaatta
    18121 accaattagt acgtaaacct cgtaaatcaa aggtggaaaa atctaattca cctgctttga
    18181 acaaaggata taacagcttt aagaaaactc aaactaacgt aaactcacct caaaaacgtg
    18241 gggtagcaac tcgtgtgggt acaatgacac ctaaaaaacc gaactcagct ttacgtaaat
    18301 atgcacgtgt tcgtttgtca aacttaattg aagttactgc ttatatccca gggattggtc
    18361 ataacttaca agaacacagt gttgtattat tacgcggtgg tcgtgtaaaa gacttaccag
    18421 gggtacgtta ccatatcgtt cgcggtgctt tagatactgc tggtgttaac gaccgtaaac
    18481 aaagccgttc taaatacgga actaaaaaac ctaaagctta attttaatta ttaaacaaat
    18541 gaaacttgct tctactatat agaagtaatt actatgaaca atatttcgga aggaggagtt
    18601 acggatgcca cgtaaaggtc ctgttgcaaa acgtgatgtt ttaccagatc ctatttataa
    18661 ctcaaaatta gtaactcgct tgatcaaccg tgtaatggtt gacggaaaac gcgggattgc
    18721 agctaacatt atctataatg cctttgactt gatcaaagaa tctacaggta acgatccatt
    18781 agaagtgttt gaacaagcaa tgaaaaacgt tcaacctgtt cttgaagtta aagcacgtcg
    18841 tgttgggggt tcaaactacc aagtaccagt tgaagttcgc cctgaacgcc gcgttactct
    18901 aggtcttcgt tgggtagtaa actacgctcg cttgcgcggt gaacacacaa tggaagaacg
    18961 cttagcaaaa gaaatcatgg atgctgcaaa caacacaggt gcttctgtta aaaaacgtga
    19021 agacactcat aaaatggctg atgccaaccg tgcgtttgct cactatcgct ggtaagattc
    19081 tctctgtttg gtcggctttt ataagtcgac ctcagatagt gtccttattt ttagcaacta
    19141 gatagtaaag agaggagaaa caaggagaat gaccagagaa ttttctttac aaaacactcg
    19201 taatatcggt atcatggccc acatcgatgc tggtaaaacg actgctactg aacgtatctt
    19261 gtactatact ggtaaaatcc ataaaattgg tgaaactcat gaaggagctt cacaaatgga
    19321 ctggatggaa caagaacaag aacgtggtat tacgatcact tctgctgcta ctacagcaca
    19381 atggaagggt caccgaatca acatcatcga cacaccagga cacgtagact tcacagttga
    19441 agttgaacgt tcattgcgtg tacttgatgg tgctgtaact gttcttgatg cacaatcagg
    19501 tgttgaacct caaactgaaa cagtttggcg tcaagcaaca acttatggtg tacctcgtat
    19561 cgtatttgca aacaaaatgg acaagatcgg tgctgacttc ttatattcag tatcaacttt
    19621 acatgaccgt ttgcaagcaa acgctcaccc aatccaatta ccaattggtg cggaagacga
    19681 cttcacagga attatcgacc ttgtaacaat gaaggctgaa atgtatacaa acgatcttgg
    19741 aacagagatc gaagaaacag aaattcctga agaataccgc gaattagctg aagaatggcg
    19801 cgaaaaattg gttgaagctg tagctgaaac tgatgaagaa cttactttga aatacttaga
    19861 aggtgaagaa atcactgaag ctgagttaaa agaaggtatc cgccgcgcaa cagttaacgt
    19921 agaattctac ccagtactat gtggttcagc atttaagaac aaaggggttc aattactatt
    19981 ggacgctgtt cttgattact tgccatcacc gttagatatc ccagctatca aagggattga
    20041 ccctaaaact gatgaagaag tagaacgtcc tgcagacgac agcgctcctt tctcagcttt
    20101 agcatttaaa gttatgactg acccattcgt aggtcgttta actttcttcc gtgtgtactc
    20161 tggtgtttta caatcaggtt catacgtaca aaacgcaaca aaaggcaaac gtgaacgtgt
    20221 tggtcgtatc ctacagatgc atgccaactc tcgttcagaa atctctgaag tttacgctgg
    20281 agatatcgct gctgctgttg gtttgaaaga cacaactact ggggacacac tatgtgatga
    20341 aaagagtcta gttattcttg aatccatgga attcccagaa ccagttatcc aagtcgctat
    20401 tgaacctaaa tcaaaagctg accaagataa aatgggtgtg gctttacaaa aactatctga
    20461 agaagatcct actttccgtg ctgaaacaaa cgtggaaaca ggcgaaacaa tcatcgctgg
    20521 tatgggtgaa cttcacttag atatcatcgt tgaccgtatg cgtcgtgaat tcaaagttga
    20581 agctaacgta ggtgcgcctc aagtatctta ccgtgaaact ttccgtgctg gcactcaagc
    20641 cgaaggtaag tttgtacgtc agtctggtgg taaaggtcaa tacggtcacg tatggattga
    20701 atttacacca aatgaagaag gtgctggttt cgaatttgaa aatgcgatcg tcggtggggt
    20761 tgttcctcgt gaatacattc cagctgttga aactggtttg aaagatgcta tggaaaatgg
    20821 tgtcttagct ggctatccat tagtagatat caaagctaaa ctttacgatg gttcatacca
    20881 tgatgtcgat tcaaatgaaa ctgccttccg tgttgctgct tctatggcat tacgtgcagc
    20941 agctaagaaa gctaatccag taatccttga accaatcatg gctgtagaag ttgttatccc
    21001 tgaagattat ttaggtgatg ttatgggtca cgtaactgct cgtcgtggac gtgttgaagg
    21061 tatggaagct cgcgctggcg gccaacaagt tgttcgtgcg atggttccac ttgctgaaat
    21121 gtttggttat gctacaactc ttcgttctgc tactcaaggt cgtggtacat ttacaatgac
    21181 atttgaccac tacgaagatg taccaaaatc agtacaagaa gaaatcatta agaaaaacgg
    21241 cggaaaagct gaataatttc ttgaaaccgg atagtcctgc aaagagatta tccaaagaga
    21301 ctatattttt aatatagttt cgtgtaaaat aattatcgat gatatgggca tgtggtgtac
    21361 cttgtgtaca tcatatacct atcagacaaa taaaaaatat attgtatttt taggaggaca
    21421 ttttaaaatg gcaaaagaaa aatttgaccg ttctaaacca cacgttaaca ttggtactat
    21481 cggacacgtt gaccatggta aaactacatt aacagctgca atcacaactg tactatctaa
    21541 gaaaaacggc ggccaagcta tggcttacga tcaaatcgat ggtgctccag aagaacgcga
    21601 acgcggaatc acaatctcaa cagctcacgt tgagtatgaa acagatacac gccactacgc
    21661 tcacgttgac tgcccaggac acgcggatta cgttaaaaac atgatcactg gtgctgctca
    21721 aatggacgga gctatcttgg tagtttctgc tgctgacggc ccaatgcctc aaactcgtga
    21781 acacatccta ttgtctcgtc aagttggtgt tccttacatc gttgtattct tgaacaaagt
    21841 agacatggtt gatgacgaag aattactaga attagttgaa atggaagttc gtgacctatt
    21901 aacagaatac gaattccctg gtgacgatgt tcctgtagtt gctggatcag ctttgaaagc
    21961 tctagaaggc gacgcttcat acgaagaaaa aattcttgaa ttaatggctg cagttgacga
    22021 atacatccca actccagaac gtgacaacga caaaccattc atgatgccag ttgaagacgt
    22081 gttctcaatt actggacgtg gtactgttgc tacaggtcgt gttgaacgtg gacaagttcg
    22141 cgttggtgac gaagttgaag ttgttggtat tgctgaagaa acttcaaaaa caacagttac
    22201 tggtgttgaa atgttccgta aattgttaga ctacgctgaa gctggagaca acattggtgc
    22261 tttactacgt ggtgttgcac gtgaagacat ccaacgtgga caagttttag ctaaaccagg
    22321 tacaatcaca cctcatacaa aattctctgc agaagtatac gtgttgacaa aagaagaagg
    22381 tggacgtcat actccattct tcactaacta ccgtccacaa ttctacttcc gtacaactga
    22441 cgtaacaggt gttgttgaat taccagaagg aactgaaatg gttatgcctg gcgacaacgt
    22501 aacaatggaa gttgaattaa tccacccaat cgctatcgaa aacggtacta aattctcaat
    22561 ccgtgaaggc ggacgtacag ttggtgccgg tgtcgttaca gaaatcaaag cttaattcac
    22621 ttaagtttta aagtaaagca acatttattc ggacgtaatc gctcggaacg aaggtcttag
    22681 gatctttgtt ccgagctttt ttatttattg caggatcagg aaaaaagaca ggagtattat
    22741 aaatagaaga aacacgaaat aaaagagtca gtattttcta aatgaaactt tatgaaaaca
    22801 aaatgttttt tacaagaaaa agccttgctt tgtgagttta tatatattat aatgacaaag
    22861 gtgcgtttac tttagtgaat tgcacagacg taatcagaat gatttcgtcg gctaagaaac
    22921 gagaggttgc gacacgcccg gacgctttgc cacgaacgag cgtgacggaa attttcgtgg
    22981 agctatgtct acttttcaaa ataggcgaag gagggaacaa gatggcaaaa caaaaaattc
    23041 gtatccgttt aaaagcgtat gaacaccgta ttttagatca atcagcggat aaaatcgtgg
    23101 aaactgcaaa aagaactgga gctagcgtgt ctggtccaat tccattacca actgaacgca
    23161 gtctttacac aatcatccgt gcgactcata aatacaaaga ttcacgcgaa caattcgaaa
    23221 tgcgtacaca caaacgtctt atcgacattg tgaacccaac accaaaaact gttgatgctt
    23281 tgatgaagct agacttacca tctggtgtaa acatcgaaat caaattataa aactaaaaca
    23341 aaaataatgg aggtgtactc atgaccaaag gaatcttagg gaaaaaagtg ggaatgacac
    23401 aaatcttcac tgaatctggt gaattaattc cagtaactgt tgtagaagct actccaaacg
    23461 tagtactaca agtgaaaaca atggaaacgg atggctacga agctattcaa gttggttacc
    23521 aagatatgcg tgaagtttta tcaaacaaac ctgcgaaagg tcatgttgca aaagcaaaca
    23581 cggctcctaa gcgcttcatt cgtgaattca aaaatgttga gctaggagaa tatgaagtag
    23641 gaaaagaaat tacagtagat gttttccaag caggagacat tattgatgta acaggtacta
    23701 cgaaaggtaa aggattccaa ggggttatca aacgccacgg acaaagccgc ggaccaatgg
    23761 ctcacggttc tcgttaccat cgtcgtcctg ggtcaatggg tccagttgca cctaaccgtg
    23821 tatttaaaaa caaacgtctt gctggccgta tgggtggcaa ccgcgtaact atccaaaact
    23881 tggaagttgt acgtgtcgat gcagaaagaa atgttatctt gatcaaaggt aatgttcctg
    23941 gagctaaaaa atctttaatt actatcaaat cagctgtgaa agctaaataa ttgtaggaag
    24001 aaaggaggaa ctaaggaatg ccgaatgtag cattattcaa acaagatgga agccaaaacg
    24061 gtgaaatcac acttaacgaa gaaatcttcg gaattgaacc taatgaaagt gttgtttacg
    24121 atgcaatcgt aatgcaacgt gcgtcattaa gacaaggaac acacgcggtt aaaaaccgta
    24181 gcgctgttcg cggcggcgga cgtaaaccat ggcgtcaaaa aggaacaggt cgtgcacgtc
    24241 aaggatctat ccgctcacca caatggcgtg gaggtggcgt agtctttgga ccaacaccac
    24301 gttcttacag ctacaaactt cctaaaaaag ttcgtcgttt agcaatgaaa tctgtattgt
    24361 cagaaaaagt tgctgaaaac aacttggtag caatcgaagg cttgaacttc gacgcaccaa
    24421 aaacaaaaga attcaaacaa gttcttgcta acttatctat cgactctaaa gtattggttg
    24481 ttctagaaaa aggtaacgac tttgctgctt tatcagcacg taacttacca aacgtttctg
    24541 tagtaacttc tgataatgtg agtgtgctag acgttgtatc aaatacaaaa gtgttagcaa
    24601 cacaaactgc tcttactcaa attgaggagg tgcttgcata atgaacttac tagacgtaat
    24661 caaacgccca gtgatcactg aaaaatcaat gcttgccatg gatgacaaga aatacacttt
    24721 tgaagtggac actcgcgcaa acaaaacttt agtaaaacaa gcggtagaag ctgcttttga
    24781 cgttaaagtc aaaaacgtga acatcgtgaa cgtacgtgcg aaattcaaac gtatgggcaa
    24841 atatgcagga tatacaaaaa aacgtcgcaa agcgatcgtt acattgactg aagattcaaa
    24901 agaaatccaa ttattcgaag ctgctgaata agcactaata gataactcat taaattagga
    24961 gggaaaaacg tggcgattaa aaagtacaaa cctacctcaa atggtcgtcg taacatgaca
    25021 ggttctgatt ttgctgaaat cacaacatca acacctgaaa aatcattgtt acaaccattg
    25081 aaaaaccatg ctggacgtaa caacaacggc cgcatcactg tacgtcatca aggtggcgga
    25141 cacaaacgcc aatatcgcgt gatcgacttc aaacgtaaca aagataacgt tgttgcaact
    25201 gttcaaacga tcgaatatga tccaaaccgt tcagctaaca tcgcgttagt acactatgaa
    25261 gatggagtga aagcttacat cttagcacct aaaggactag aagtgggtat gaaacttgtt
    25321 tcaggtccag aagcagatat caaagtcgga aatgctcttc cattagaaaa cattccagtt
    25381 ggtactgtta tccacaacat tgaaatgaaa cctggtaaag gtggacaatt gatccgttct
    25441 gctggtacaa gtgctcaagt acttggtaaa gaaggcaaat acgtattgat ccgcttgaac
    25501 tctggtgaag ttcgtatgat cttagctgca tgccgtgcta caatcggttc tgtaggtaac
    25561 gaacaacacg aattaatcaa catcggtaaa gctggacgct ctcgttggat gcgtaaacgc
    25621 ccaactgtac gtggtagcgt aatgaaccct aatgatcacc cacacggtgg tggtgaaggt
    25681 aaagctccta tcggacgtaa agcaccagtt tctccttggg gtcaaccagc tcttggttac
    25741 aaaactcgta acaaaaaagc taaatcagac aaacttattg ttcgtcgtcg caaaaataaa
    25801 taattacttt gaccatgtgt cgcacatttt gagaggaggt tcaccatggg tcgtagttta
    25861 aagaaaggac cttttgtcga tgagcattta atgaaaaaag ttgaagctca agcaggggtt
    25921 gaaaagaaaa aagttatcaa aacttggtca cgccgttcaa cagttttccc aagttttgtt
    25981 ggatatacca tcgcagttta tgatggacgt aaacatgtac cagtttacat ccaagaagac
    26041 atggtaggac acaaattagg tgaatttgca cctacaagaa cttatcgtgg acatgctgct
    26101 gacgataaga aaacgaaacg ctaatttgag gggaggataa atccaaatgg cagaacaaat
    26161 cacatcagct aaagcaactg caaaaacagt tcgcacttca cctcgaaaag cccgtttagt
    26221 aattgacctt atcagaggta aaagtgtcgc agatgcaatt tcaatcttga aattcacacc
    26281 aaacaaatct gctggaatca ttgaaaaagt attgatgtca gcagttgcga atgcagaaaa
    26341 taactttgac ttagatgttg aaaacttggt agtaagcgaa gcttttgtta acgaaggacc
    26401 tacaatgaaa cgtttccgtc ctcgtgcaaa aggatcagct tctccaatca acaaacgtac
    26461 aagtcatatc acggtagtcg tatcagaaaa ataaggaggg ataatcagtg ggtcaaaaag
    26521 tacatccaat tggaatgcgt gtaggcatca tccgcgactg ggatgctaaa tggtatgctg
    26581 aaaaagagta tgctgagttc ttacacgaag atttaagaat ccgtaaattt atttcaacta
    26641 aacttgctga tgctgctgta tcaacagttg aaatcgaacg cgcaacaggt cgcgtaaaca
    26701 tttcaattca cacagctaaa ccaggtatgg ttattggtaa aggcggttct gaagtcgaaa
    26761 gcctaagaaa agaattaaac aaattaactg gtaaaagaat ccatatcaat atcgtggaaa
    26821 tcaaaaaacc agatctagat gcaaaattag taggtgaagg aatcgcacgt caattagaaa
    26881 accgtgttgc tttccgtcgc gctcaaaaac aagccatcca acgctcaatg cgtgctggcg
    26941 ctcaagggat caaaactcaa gtatctggtc gtctaaacgg agcagatatc gctcgttcag
    27001 aaggatactc agaaggaact gttcctcttc atactttacg tgcggacatt gattacgcat
    27061 gggaagaagc agatacaaca tacggaaaac taggagttaa agtgtggatc tatcgtggag
    27121 aaattcttcc aactaaaaaa aacactgaga aaggagggaa ataatcatgt tagtacctaa
    27181 acgtgtaaaa caccgtcgtg aattccgcgg aaaaatgcgc ggggaagcta aaggcggaaa
    27241 agaagtagca ttcggtgaat acggtttgca agctgttgat tcacattgga tcacaaaccg
    27301 ccaaatcgaa gctgctcgta tcgcaatgac tcgttacatg aaacgtggtg ggaaagtatg
    27361 gattaaaatt ttccctcaca aatcttatac tgccaaagca attggggtac gtatgggttc
    27421 tggtaaaggg gcacctgaag gatgggttgc accagtaaaa cgtggtaaaa tcatgtttga
    27481 aatcgcaggc gttcctgaag aagtagctcg tgaagcgtta cgtctagctt ctcacaaatt
    27541 accaatgaaa actaagatcg taaaacgtga ggaaatgggt ggtgaatcga atgaaggtta
    27601 aagaaatcag agaattaacc actgccgaaa tgcttgatca agaaaaacaa ttaaaagaag
    27661 aattgtttaa tcttagattc caattagcaa caggtcaatt agaaaacact gcacgtatta
    27721 aagaagtacg taaatcgatt gcacgcatca aaactgtgtt gcgtgaacaa gctaagtaat
    27781 tatggaagga ggccattaag cgtatgactg aaggaagaaa tcaacgcaaa gtttatcaag
    27841 gtcgcgtggt ttcagacaaa atggataaaa caattaccgt tgctgtcgaa acaaagaaaa
    27901 accaccctat ctatggcaaa cgcatgaact attctaagaa atacaaagtg catgatgaaa
    27961 acaacacagc aaaagttggc gacatcgtga gaattatgga aactcgtcca ttatcagcta
    28021 caaaacgttt ccgtttgttg gaagtagtcg aagaagcagt tattatctaa taacacgaga
    28081 ttttcctata tagattgaat gcaaaatctg aaaggaggat acacaacgtg atccaagcag
    28141 aaagtcgttt aaaagtcgct gataactcag gcgcacgtga aattttgacg atcaaagtcc
    28201 ttggtggatc tggtcgtaaa actgctaaca tcggtgatgt gatcgttgct acggttaaac
    28261 aagcaacgcc aggtggggtt gtcaaaaaag gtgacgtcgt taaagccgtt atcgttcgaa
    28321 caaaatcagg agctcgtcgt acagacggtt catatattaa atttgatgaa aatgctgcag
    28381 tgattattcg tgatgataaa agccctcgtg gaacacgtat ctttgggcca gttgcacgtg
    28441 aactacgtga aaacaacttc atgaagatcg tttcactagc accagaagtt ttataatctt
    28501 gagacactta tcaaaggagg tgcgaaacag ttatgtttgt taaaaaaggc gataaagtta
    28561 aagttatcac tggaaaagac aaaaacaaag aaggcgttgt actagcagcg ttcccgaaaa
    28621 aagataaagt tattgtcgaa ggtgtaaatg tcgttaaaaa acaccaaaaa ccttctcaag
    28681 ctgctcctca aggtggaatt gttgagatgg aagcgccaat tcatgtttct aacgtgatgg
    28741 tggttgactc tactggtgta gctggccgag ttggctacaa agaagtagac ggcaaaaaag
    28801 tccgtgtttc taaaaaaacc ggtgaagttt tagataaata aaagtttaag gaaggagggt
    28861 cttactgaat gaaccgccta aaagaaaaat atcttaaaga agtaactcca tcattgatgg
    28921 aaaaatttaa ctatagctct gttatgcaaa cacctaaagt tgaaaaaatc gtcatcaaca
    28981 tgggtgttgg tgatgcggta tcaaacgcta aaaacttaga taaagcagtt gaagaattaa
    29041 ctttgatcac tggtcaaaaa ccaatgatta caaaagctaa aaaatcaatc gctggattcc
    29101 gtttacgtga aggaatgcca attggtgcaa aagttacatt gcgcggagaa agaatgtacg
    29161 aattcttaga caaattggtt tctgtttcac taccacgtgt acgtgacttc catggtgtaa
    29221 gtaaaaaagc ctttgacggt cgtggaaact atactttagg tattaaagaa caattgatct
    29281 tcccagaagt agattatgat ttagtagata aagtacgtgg tatggacatc gttattgtaa
    29341 caacagcaaa ctctgatgaa gaatctcgtg agttgttgac acaattaggc atgccattcc
    29401 aaaaataatt aaaggaggcg aactacgtgg ctaaaaaatc aatgattgct aaaaacaaac
    29461 gtcctgcaaa attttcaaca caagcttata ctcgttgcga acgttgcgga cgtccacatt
    29521 cagtttatcg taagttccat ctttgccgta tttgcttccg cgaacttgcc tataaaggtc
    29581 aaattcccgg cgtgaagaaa gctagctggt aaaaagtaaa ttcgcataag aaggaggtaa
    29641 aagatcaatg gtcatgacag atccaattgc agattttcta actcgtatcc gtaacgcaaa
    29701 catggttaaa catgagtctt tagaagtacc tgcatcaaaa attaaacgtg acatcgctga
    29761 aatcttgaaa cgtgaaggtt tcgttcgtga tgttgaatac atcgaagatg acaaacaagg
    29821 cgtgatccgt gttttcctaa aatttggtaa aaacggcgaa cgtgttatca caaacttaaa
    29881 acgtatctct aaaccaggtt tgcgtgctta tgtcaaagct gacgaagtac caaaagtatt
    29941 gaacggttta ggtatcgcaa tcatttcgac ttctgaaggt gttgtcactg ataaagaagc
    30001 tcgtgaaaaa aatatcggcg gcgaagttat cgcttacgta tggtaatcaa gtagaatcta
    30061 caaggaggtg tctttaagtg agccgtatcg gaaataaagt cgttgttatc cctgaaggag
    30121 taacagttac tcaagacgga aacaacgtta cagttaaagg accaaaaggt gaattgactc
    30181 gtactttttc agcagatatc aaaatgaata tcgaaggaaa cgaagtaact ttcactcgtc
    30241 caaacgacag taaagaaatg aaaacaattc atggaacaac tcgtgcgaac ttcaacaaca
    30301 tggttgtagg tgtcagcgaa ggtttccaaa aagcgttaga actaatcggg gttgggtacc
    30361 gtgcacaatt acaaggatca aaacttgtat tgaacgttgg ttactcacat ccagtagaaa
    30421 tcacaccacc agctggagta actgttgaag taccagctaa cacacaagta attgttaaag
    30481 gtgcgaacaa agaagaagtt ggcgaattag ctgcaaacat tcgtggaact cgtcctccag
    30541 aaccttataa aggtaaaggt attcgttatg ttggcgaata tgtacgccgt aaagaaggta
    30601 aaactggtaa ataatcgttt agaaaaattg catagaagcc agcagcagga aaaaggcatt
    30661 tggctgaagt ttaccttcaa aaaaatacat ttttacttgt gccgcagctt ctatttttcg
    30721 aaacgtattt actgcttgaa atagtagtac aataaaaata aagaggtgac aattgtgatt
    30781 acaaaaccag ataagaacaa aacacgtcaa aagagacatc gtcgtgtacg taacaatatc
    30841 tctggtactg ctgagcgccc acgcttgaac gtttttcgtt ctaacaaaaa catctacgct
    30901 caaattattg atgacgtagc gggtgtaacg ctagcaagtg cctctacctt ggataaagaa
    30961 atttcaggtg gaacaaaaac agaagcagca caagctgtcg gtaaactagt tgctgaacgt
    31021 gcagctgaaa aaggcattaa agtagtagtc tttgaccgtg gtggatacct ttaccatggc
    31081 cgtgtgcaag ctttagctga agctgctcgc gaaaatggac tagaatttta gaagaaggag
    31141 gaataccatt catggtttat atcgatccaa aacatttgga attagaagac cgcgttgttg
    31201 caattaaccg tgtaacaaaa gttgttaaag gtggacgtcg tctacgtttt gctgctttag
    31261 ttgttgtcgg tgacaaaaac ggacacgtag gatttggtac aggtaaagct caagaagttc
    31321 ctgaagcaat ccgtaaagca attgaagatg cgaagaaaaa cttagttgaa gtacctatgg
    31381 ttggttcaac aatcccacac gaagtaatcg gtgcttttag tggtggtaga atcctaatga
    31441 aacctgctgt agaaggttct ggggttgctg ctggtggacc agttcgtgcc gtattagaat
    31501 tagctggggt agcagatatc acttctaaat ctttaggctc aaatacacca atcaacgttg
    31561 ttcgcgcaac agttgaaggt ttgaaacaat tgaaacgtgc agaagaagtt gcagaacttc
    31621 gcggcaaatc agttgaagaa ttaatcggat aaggaggaca acaataatgg ctgaattaaa
    31681 agttacttta aaacgcagta ttatcggacg tcctcaaaac caacgcgata cagttaaagc
    31741 gttaggtctt acaaagatta acagttcagt ggttaagcct tctaatgaag caattaaagg
    31801 catgatcaat actgtttctc acctagtgga cgttgaagaa atttaattta gaaaaatcat
    31861 aactagaaca ttaaggaggt gccaaaccta tgaaacttca tgaattaaaa cctgctgaag
    31921 gttcacgcca agtacgtaac cgtgttggtc gtggtacttc atctggtaac ggaaaaactg
    31981 ccggacgcgg acaaaaagga caaaaagctc gttcaggcgg cggtgtgcgt ttaggatttg
    32041 aagggggaca aacaccattg ttccgtcgtt taccaaaacg aggattcaca aacgtgaacc
    32101 gtaaagacta tgcagtcatt aatcttgatg tattgaaccg ctttgaagac ggagctgaag
    32161 taactcctgt tgctttagtt gaagctggaa tcgtgaaaaa cgaaaaagct ggaatcaaag
    32221 tattagctaa tggggaacta aacaagaaat taactgtgaa agcagcaaaa ttctctaaag
    32281 cagctcaaga agcaattgaa gcggctggtg gatcaatcga ggtgatctaa tgtttagact
    32341 attaaaagat gcttttaaag taaaagacat tagatcgaaa atcctattta ctgtatttgt
    32401 gttgtttgta tttcgcctag gtgcacatat tactgtacca ggcgtaaatg ctgcttcatt
    32461 gcaaaacctt tcttctttgc catttttcaa catgttgaac ttggtaagtg gtagtgcgat
    32521 gcaaaacttt tccattttct caatgggggt ttcaccatac attactgctt ctatcgtcat
    32581 tcagcttctt caaatggaca tcgtgcctcg atttgttgaa tggtcgaaac aaggggaagt
    32641 tggtcgtaaa aaactgaatc aagcgactcg atatctgaca ttagtattgg catttatcca
    32701 atctgtcggt atcactgctg gattcaatca atacgctcaa ttaggtttcg tgaataatcc
    32761 taacgtagca acttacgtaa cgatcggttt gattctaaca gccggtacga tgtttgttac
    32821 ttggctagga gaacagatca cagaaaaagg tatcggaaat ggcgtatcaa tgatcatttt
    32881 tgcaggtatc atttctagat tgcctgaaag tatcagagaa atttatgaag attactttat
    32941 aaatattgat ccttcagaca tatggaaatc tgctattttt gtggccattt tactagtagc
    33001 ggtcttgttg atcgtgacat tcgtaacatt tttccaacaa gctgaacgca agatcccgat
    33061 ccaatatacg aaacgcgtag ctggcgcgcc tacaagcagc tatctgccgt tgaaagtaaa
    33121 tgcagctgga gttattccag ttatctttgc tagttcgttt attgctacgc caaatgctgt
    33181 tttacaagca ttgagcggta gattcggttc tgaaaactgg tacaaagtga tgatggagat
    33241 ctttaactat aatacagcac ctggtgctac gatttatacg attctgatcg ttgccttcac
    33301 tttcttctac gcctttgttc aagtaaaccc tgagaaatta gcggaaaact tgcaaaagca
    33361 aggaagttat ataccaagcg tacgccctgg taaggggact gaagagtatg tctcaagatt
    33421 actgatgcgt ttaagtacag ttggtgcagt cttccttggt ttagtggcat tgcttccaat
    33481 cattgctcaa atggtttggg atcttccaca atcaatcgga ttaggcggta caagcttact
    33541 gatcgttatc ggtgtagctt tagaaactgc gaaacagtta gaaggcttaa tgctgaaacg
    33601 taaatatgtt ggattcattg agtaagaaag cgtgttgagg tttcccctca acacacgttc
    33661 tcacacattt attaggaggg aaaacaatga acctcatttt aatggggctg ccaggcgcag
    33721 gtaaaggaac acaagctgaa aaaatcattg atgcttatgg tattccgcac atctcaacag
    33781 gagatatgtt ccgcgcagcg atgcaaaacg aaacagctct tggcctggaa gcaaagtctt
    33841 atatggataa aggtgcatta gttcctgatg aagtaacaaa tggaatcgtg aaagagcgac
    33901 tagctgaacc agatacagag aaaggcttct tattagatgg ctttccacgt acgctagatc
    33961 aagcaaaagc tctggacgca atgctaaaag atttgaataa aaaaattgat gctgtcatcg
    34021 atatccatgt tggtgaagaa atcttggtcg aacgtctagc cggccgattt atctgcagca
    34081 attgtggagc aacttaccat aaaattttca accctacaaa agttgaagac acatgtgatc
    34141 gttgcggtgg gcatgaattc tatcaaagag aagatgataa acctgagacg gttaaaaatc
    34201 gtctggctat caacatcaaa aatagtgaac caatcttggc ttattataaa gaacaaggtt
    34261 tgctaaacac tattgatggt gctcgtgaaa tcgatgctgt atttgcagat gtacaaaaaa
    34321 tcattgaaaa atagcacgat tgcccgtagc cgacttgcat tatgcagtta ctcttgtcaa
    34381 gttcaataaa ttatgataga atatgtcagt ccgacttcta gagaatcctc tagatgatcc
    34441 attttgagaa ttttgaggtg ggaactgcag tttccgccat tttatcgtgt gaaaatgcga
    34501 aacaagtaca aggaggtact gtgcgtggca aaagaagata tgattgaagt cgaaggtaca
    34561 gtcgtcgaaa ctttgccgaa tgcaatgttt aaagttgaac tagaaaacgg acaccaagtt
    34621 cttgctactg tttcaggtaa aattcgtatg cattacatcc gtatcttacc tggtgacaaa
    34681 gtaacggttg aattgtctcc atacgatctt aaccgtggcc gtattactta tcgctttaaa
    34741 taattgtact ccgtaaaact acaggaggta ttatcatgaa agtaagacca tcagtaaaac
    34801 caatgtgcga acattgtaaa gtaattcgtc gtaaaggacg cgttatggtg atttgcccag
    34861 caaatccaaa acataaacaa cgtcaaggat aattggaggt gctataaata tggctcgtat
    34921 tgcaggagta gatattcctc gtgataaacg tgtagtagtt tcacttactt atatctatgg
    34981 tattggtaac actactgcta aaaagatctt agctgatgct ggtgtatctg aagatgttcg
    35041 cgttcgtgaa ttaacgaatg aacaaacaga tgctatccgt gcagaagtag ataaattaaa
    35101 agttgaaggg gatcttcgtc gtgaagtgaa cttgaacatc aaacgtttga tggaaatcgg
    35161 ttcttaccgt ggaatccgtc atcgccgtgg attgcctgtt cgtggacaaa acacgaaaaa
    35221 caatgcacgt actcgtaaag gcccagctcg cgctatcgct ggcaagaaaa aataattctt
    35281 aagtgaagga ggttaaaact tcatggcagc aaaaaaagtg aatcgtaaac gccgtgtgaa
    35341 gaagaatata gaagcaggta ttgcacatat ccattctaca ttcaataaca ctatcatcat
    35401 gatcactgat actcacggaa atgcattagc gtggtcatca gctggttcat taggtttcaa
    35461 aggaagcaaa aaatcaactc cttttgccgc tcaaatggca gcagaaactg ctacaaaagc
    35521 agcaatggaa cacggattaa agactgttga agtaacagtt aaaggacctg gttctggacg
    35581 tgaagcagca attcgttcat tacaagctac aggtttagaa gtgactgcaa ttcgtgacgt
    35641 gactccagtt cctcataatg gtgcccgccc tccaaaacgc cgtcgtgttt aatgagtgcc
    35701 gctcattttg agttttatca gtaacgtcat tgaacaagac acttttcgtt ttgaaagggg
    35761 taaagataag aatgattgag ttcgaaaaac caagaatcgc aaaaattgat gaagaaaaag
    35821 attatggcaa gttcatcgtt gaacctcttg aaagagggta tgggactact ttagggaatt
    35881 ccctacgtcg tattttatta tcttctttgc caggtgctgc catcactaat attcaaatcg
    35941 atggtgtact gcatgaattc tctaccatca aaggtgttag agaagacgtc actcaaatca
    36001 tcttgaacat taaaggctta gcgctgaaaa tgtatggcca agaagaaaaa acccttgaaa
    36061 tcgatattac cggacctgct acagtaactg ctggcgatat tatcgttgat agtgatgtag
    36121 aaattcttaa caaagatatg tacatctgta gtgtctctga aggagctacg ttccatgctc
    36181 gcttaacagt gaaacctgga cgtggttatg ttcaagcaga tgaaaataaa aaagaagata
    36241 tgccaattgg cgttcttcca gtcgattcaa tctacacacc tgttcgtcgt gtgaactatc
    36301 aagttgagaa cacacgtgtt ggacaccgtg aagattttga taaattgacg atggagattt
    36361 ggacagatgg ttcaattgaa ccgttggaag caatgagttt gtctgcgaaa atcatgactg
    36421 aacatttgga tatttttgtg aacttaactg atgaagcaaa aaatgctgaa atcatggtcg
    36481 aaaaagaaga aacacaaaaa gaaaaaatgt tggaaatgac gatcgaagag ttagacttgt
    36541 ctgttcgctc ttacaattgt ttgaaacgtg caggaatcaa tactgtacaa gaacttacaa
    36601 ataaatctga gcctgaaatg atcaaagtcc gtaacttagg acgtaaatca ttagaagaag
    36661 ttaaagcaaa attgcacgac ttaggtttag gtttacgtaa agaagattaa aaccttttac
    36721 gaaggaggga aaccaacgtg agttatcgta aactaggacg cacatctagc caacgtaaag
    36781 cgatgttgcg tgatttaaca actgatttat taatcaacga acgtattgtg actactgaag
    36841 ctcgtgcgaa agaagttcgc tcaactgcag aaaaaatgat cactttaggt aaacgcggag
    36901 atttgcatgc acgtcgtcaa gctgcggctt tcgtacgtaa tgaagttgcc agcgtgcgtg
    36961 aagaaaatga ggacatcgtc attgaatcag ctttacaaaa actgttcaac gatattgcac
    37021 ctcgttacgc agaacgtcaa ggcggataca cacgcatttt gaagacagaa ccaagacgcg
    37081 gtgatggtgc accaatggtt gtcttagaac ttgtctaaaa tcacactaat aacatcactt
    37141 tatagatgat tcttttagag tgttatgatg ttggaagata tttttccgag tctagctcag
    37201 cgctgctcct caagcgaata tcgcttgggg agtaagcact catcataaag tgttttttta
    37261 taccttcaat tacctgtcat gacagatatg tttgaaggca taaaaaaaca ttttaagcga
    37321 gaaataattg aaaattttgt ctttttttga tagtgttagt aggaacttta ggatgaagaa
    37381 ggagtaatca tggagccaat tattgaatta gaaaagataa attataaata ccagccagat
    37441 gatcttcgcc ccgcattgaa agatgtctcc tttacaattg ataaaggtga gtggatcgct
    37501 attattggac acaatggttc aggaaaatcg acacttgcca aaacgatcaa tggattgctt
    37561 ctgccagaat cagggatcgt gaaagtggga aatcaaatat tggatgaaga aaatatttgg
    37621 acgatccgac agatggttgg aatggttttt caaaatccag acaatcagtt tgttggttcg
    37681 acagtagaag atgacgttgc ttttggtctt gaaaatcaag ggattcccag agaagaaatg
    37741 ctagtgcgtg tgaaagatgc acttgagaaa gttagaatgg ctgagtttgc ttcacgcgag
    37801 cctgctcgtt tatcaggagg gcaaaaacaa cgtgttgcca ttgccggcgt ggttgcctta
    37861 cgacctgata tcattatttt agatgaagca accagtatgc tagatccaga aggtcgtgaa
    37921 gaagtgatct ctacgatcaa gaagatcaaa gaagaaagtc aattgaccgt tatatctatc
    37981 actcatgata ttgatgaagc agctaatgcc aatcggatac tagtgatgag acaaggcgaa
    38041 cttgtccgtg aaggtacacc aaaagaaatt ttttctgctg gtcctgaact gatcgactta
    38101 ggtctagatc taccatttcc tgaaaaactg aagagcgcac tgaaagaacg tggggttgat
    38161 gtgcccagtg aatatatgac agaagaaagg atggtggatt ggttatggac atccgttttg
    38221 aacaagtaga cttcacttat cagccgaata caccatttga acaaagagcc ttatttgaca
    38281 tcaatatgac gatcaaagaa aacagttata ctgcattagt agggcatacc ggaagcggga
    38341 aatcaacctt acttcagcat ttgaatgcgc ttgttaagcc aacaagcggt acagtccata
    38401 ttggagaacg agatattcag ccggatacgg ataataagaa tttaaagccc atccgaaaaa
    38461 aagttggcat cgtctttcag tttccagaag cacagctatt cgaagaaacg gtagcaaaag
    38521 acattgcttt cggtcctaaa aactttggtg tcagcgaaga agaagcacta gtcctagcaa
    38581 aagaaacatt agaacaagtt gggctggatg aaagctattt ggaacgttcg ccatttgaac
    38641 tttcaggagg gcaaatgcgt agagtagcaa tcgctggtgt gcttgccatg aggccggaag
    38701 tacttgtatt ggatgaacca acggcaggac ttgatcccca gggcagaaaa gaaatgatgg
    38761 agatgttttg gcgtcttcat aaggaacagc agatcactat tgtattagtt acgcatttaa
    38821 tggatgatgt agccaatttt gcagattatg tctatgtgtt ggaaaaggga cgaatcgtta
    38881 atagcgggga gccgcaagag gtctttcaaa acattgaatg gctaaaagaa aaacaactag
    38941 gcgtacctac tgccaccgag tttgcagaag aattaatggc taaagggatg aactttgcta
    39001 ccttgccttt gacagctgaa gaattagcag acgctatcgt ccattatgct ggaggaaatg
    39061 cccatgatga ataaattgat atttggtcgt tatatgcctg gagattcctt gatccatcga
    39121 ttggatcccc gagctaaatt attagccagt ttttatttta tcggaatcat ttttttagca
    39181 aataattggg aaacgtacct tttgttggca tttttcacat tactttctgt cttcttgtca
    39241 aaagtagatt tgaaattttt tatccgaggc attcgcccat tgatctggct aatcctcttc
    39301 actgtagctt tgcaggtctt gtttacaagt ggcggagaaa cttattggag ttggggtgtt
    39361 ttccatatca ctgaatttgg tgtgatgaac ggcctcttta ttttctgtcg attcgtgttg
    39421 atcattttta tgtcgactct actcacgctt actacaccgc ctttggaatt atcggatgcg
    39481 attgagtact tgcttcgtcc attgaaagcc gtacggttcc cagtccacga aatttcattg
    39541 atgctgtcga ttgctcttcg ttttgtgcct acattgatgg acgaaacaga aaagatcatg
    39601 aacgctcagc gcgctcgcgg tgttgatttt ggtgaaggaa acctagttca gaaaatgaaa
    39661 gcagtcattc ctttgctgat tcctttattt gtcagcagct tcaaccgtgc ggaagatctt
    39721 gcgactgcta tggaagcaag aggctacaaa ggtggggaag gaagaacgaa atatcgtgta
    39781 ttgcattggc ataatagaga cacgatggtt atcattgctt ttgtattatt aactgtgcta
    39841 ctgatctttt taagaggata aacgttcagt gggccgctat ctattctttt cttgctgatc
    39901 tgtttgcaag aaaggatgca agcagtgtat gacaagaaat attagggtct gtggcagact
    39961 aaggtcacag gcctctttct tttatctgaa aagaaagaac gaaaaaattt tatagaaaaa
    40021 tcattattcc agataacctt ttaggaacaa aggagagaaa aacatggtac gttacaaagc
    40081 aatcattgcg tacgacggaa caaattttaa tggattccaa aaacagccaa atgggcgtac
    40141 tgttcaagaa gaagtagaaa aaacattaca aaaaatggca aatggaaaag aaatcacggt
    40201 ctttggttcg ggaaggacag atgccggagt acatgcgatc gggcaagtaa tccattttga
    40261 ttatccagag gagcgtccgt tagaacgaat gcgtttcgcg ctagataccc agtcaccaga
    40321 ggatattgct gtaagacaag tagaaattgt ttcagaagac ttccacgcaa gatatttggt
    40381 caaagaaaag acgtatcaat ttcgagtgga tatagggaaa ccacgcagtc cttttcgacg
    40441 ccattatgca agttattttc cttacccact tgatttggaa aaaattcagc gggcgcttcc
    40501 tgatttatta gggactcacg atttcacctc tttttgtgca tcaggaagct cgatagagga
    40561 taaaatacgt acgatctacg aggctaagat ggaggtaaat gaaacaaaag acgaattgtt
    40621 gtttacattt aggggaaatg gcttcttgta taagatgata cggatcttag taggaacgct
    40681 tttgaaaatt ggcaatggcc gattgcctga agatagtatc ccagatatta ttgcgaaaaa
    40741 agatcgtaat gcagcaggac ctacagcaca ccctgaagga ctttatttgt atgaagtgaa
    40801 atatgaataa gaggttatga tagaagtggt cagcttcaag aaataagaag gtatttacga
    40861 aaattacttc tcaaaatttt gcaaaatttc ggcttatttt cgaagaagct acttctggaa
    40921 caccgtttaa ggaagaggtc gtgaaatcag cgttttgacc tgagtattag agaaacaaac
    40981 gggaaaatag ctcctcatat tttgccgttt gttgagctaa taccgaaggt cagctgattg
    41041 aacaccgttt aagaaagagg tcgtgagcct gtcatttaac tctgagtcac aaagagcaat
    41101 ttcaaaaaac agcttctcat ctttgatgaa tatcgtgact tgtggacgag gcgttgataa
    41161 gtgaatcatc tttttgttac ctattttttc tgtaaccaaa aaacgatgga acgttgtttt
    41221 gattgccctt ggtgcggaca gtctgaacag gttccatcgt ttctttattt tctacgtctt
    41281 cttctgtgtg tgtttcgtgg ttgttcttct attgctggat caatcgtgcc ataaaagggg
    41341 cgattgcggt caaggtacca gacataaaga ataaacaatg caatacatag gacaaaaagg
    41401 attgcaccta tcgtaaatcc ttcaggcaag taactgaaag tgacagtatg tttacctgct
    41461 ggaactttta tcatcaagaa tgcgtctttg aaattgctga ccttgactgg tttcccatca
    41521 attttagctt tccatccttt gtcataagga atagttgtca ccaacatgga atcttcagcg
    41581 gtatttacag tcccagaagc ttttctgcca gtggcttcca tttcgacacc tttttctttg
    41641 atctgattca ttgcggcttg ataggctaca acatctagac caacgacttt aggctccatg
    41701 aaacttattt ctttcgtccc ataaaaactt gcagtaaatg tcactgttgt ctcttgagga
    41761 taatagccaa gatcatagta ttgaccagaa atgttgattt gcgtttttcg cgatgtacca
    41821 ttgacagtga tcgtcgcact agaactttct aattgcgcaa aattggttgg gaacaaactt
    41881 aagtaagctt gtgtatttgc aggcacatgg acttcccaag tgagctcttt tggctggtta
    41941 tctgctgttt ctgagtaagt gacgccagtc gttgtctttg tgatcgtcgt attcaactga
    42001 ttcttcaaag taggtgtata gaatgtaaaa tactgttcat ttagaccaga caatgtattg
    42061 atcaagtttg tctgactggt caaattatct gtcactggct gctcaacatc attaacggac
    42121 aacggtgcag taaatgctag tggcaatgca ttgacattct gatacagtgt atagtcgccg
    42181 ctttgaccag cttggcggaa gccatattta ctgaacgtgc tttcgttttt agaaatgtta
    42241 tatttgattc ctgtaaaccc atccatcagc aaagtattat tgttataacg gatattcaaa
    42301 tttgtccctc ttgaacggaa acctaattta tccaggtaag aggaagaatt acgatttctt
    42361 atagaagaaa acaaactgat accactgtaa ccatagttga tcgaatcgtt tgcagatact
    42421 ggatccagat tttccaagcg gtaaaacgag tcggaatctt tttttgtttc atttacaaga
    42481 gctttcaagt caggataagg atcagtatat aaactgcggg aagcataatt ccaatcatca
    42541 agaatgccat ggatcatact gttcgtattg accagtgctt cagcagacat gatcaataat
    42601 aacaatatac tcagatgatt gaatgaaagt tttttatgct gataaaatcc cacagcaaaa
    42661 gcataaatcc ctaagaaaac cactgtcaat acaaaggaag tcatcgtcac ataggtgtag
    42721 cttccttcag gtttgatgcc ccatgccacc gcaaagagag cagctacgac caaaatgatt
    42781 cctaacaaca gcccacgatc gtcttgttct atcttttccc atccttttcc tgcaagcagg
    42841 agcactaaga aagagaacag ataggcataa cggaataaaa acatattcgg tgcatgcata
    42901 ccatgccaga acaaattcaa tggaacaaga taaaaacttg cgataagtat ggcgaaaaga
    42961 ctgccgtaca aaaatttatt tttcaacggt actttccgac tggcaaaata aaacagacag
    43021 aggactaatg gtagcagacc tgcgtaaacg aacggaatcg atccgtattt tgtcgtgtca
    43081 taaacaccaa tcatattttt catgattaga tccagataag aagtcgcttc tgttttcaac
    43141 gtagtgatct gactcagtgc ctcgccattt gaccgaagat ccagcactgc tggcaataca
    43201 atgatcattg aagcgccgcc tgccagtaaa gaagtgatac cataaggaac aatcgttttt
    43261 ttatattctg accagtttgt caatagacgg gcgataaaat ataagaaaga aaatacgcca
    43321 atcataaaac ccatataaaa actggataaa aacaagagta aataactgat aaataaaagt
    43381 ttcggccgtt taagatccat aacacgatga atccccaaaa tcactaatgg aagataaaaa
    43441 aatgcatcca gccacatgat gatctccgaa tgtgcagtga taaatgagat caatgcgtaa
    43501 ctgacgctaa gtgcaacatg cccccagcga ggaatcttga acgtacgtgc aagaaaccaa
    43561 aaagccaatc cggcacagcc gaccttaagc aaagtcagaa catacagtgc atctggcatc
    43621 atctggtttg gaaaaaagaa caccagtggc gtgaagatcc cgcctagata gtaagaaatt
    43681 aaagataaat aattcaatcc tagagaagca ttccatgtat aaaaaatact ttgctttcca
    43741 agaagaacat ttcggaagct ggcatgaaaa ttagaaaact gtgaaaaggc atcacttgcc
    43801 aaaatacttc tgctgcttcc aggatagatt ccgatactta gataaacaag tgctaaaatc
    43861 aaaaacggaa tcaggaaact agctgctgcg tagcgtccgt tttctctaaa gaatgacttt
    43921 aaattttttt tcatatttat tttccaccaa atttctatga agttgtattc agtttatcat
    43981 atccttgttt ttctctgctt aaaaagagtc tagttttcac aaaaaaataa aatttagaat
    44041 aaataacttc ataatttgtt cgtataacaa aaataaacgc aaaaaaaggg taggaatcaa
    44101 aagtcccacc cttaaaaatt tttattaaat aaaaaaccgt tcactatctg aacggtccaa
    44161 gtatcattca attaaacgcg ttcaactttt cctgatttca aagcacgagt tgacacccaa
    44221 acttttttag gttttccgtc gaccataacg cgaacttttt gtaagttagg tttaacagta
    44281 cgttttgttg agttcatagc gtgtgaacgg ttgtttccac tttttgtctt gcgacctgta
    44341 aagtaacaaa cttttgccat ggtaatttcc tcctttgcct tgatttggag caatagaata
    44401 attcagctca taccagacta acatatcata tagaatggga atttgcaagt acattttcat
    44461 agatacaatt gacaattgag aagaataatt gtaaattaaa caaggttata cctgaaattt
    44521 tgcaaggttt ttgagtactt tgggtaactg ccttttattt caagttgcgc tatgataaaa
    44581 tatgatagaa gaaaaatggg agttttttac ccgttaagga ggctcattca tggctgtaaa
    44641 aattcaaacg tcagctggaa ccattgaaat taccaatgat gtaattgcaa cagttgttgg
    44701 tggcgccgcg acagatgtgt tcggtgttgt aggaatggcc agcaaaaatc aaattaaaga
    44761 taatattact gagattttgc gtaaagaaaa ttactcaaga ggagtagttg tccgccaaga
    44821 agataatggt attgctgtag atgtttatac gattatcagc tatggtacga aaatctctga
    44881 agtttcccgc aacgtccaag aaaaagtgaa atacaatcta gaggcattat taggcgtaac
    44941 tgccaattca gttaatgtct ttgtacaagg tgttcgtgta ctgccagact aggcaggaga
    45001 ctggctggta actcagcagt cgatctgaag gaggattatc taggtgagtg taacagaaat
    45061 aagtgcaagc cagttccagg aaatggtcca ggcaggtgcg aatcgtctgc aagtaaatgc
    45121 agaatacgtc aattcattaa atgtattccc tgtgccagat ggcgatacgg gaacaaatat
    45181 gaatctatct atgactagtg gagctaaggc agtagtagat tcaacctcag aaaaagtagg
    45241 agaattagca gcaatgcttt caaaaggatt gctgatgggt gcgcgcggaa attccggcgt
    45301 tattttgtct caattgtttc gtggattttc taagcaaatt ccagatgttg tctcattaaa
    45361 tgcttctgat cttgctgcag ccttcactca tggagttgaa actgcgtata aagcagtaat
    45421 gaaaccagta gaaggaacga ttttgacagt agctagagaa gcagcgaaag ctggagaaaa
    45481 gaaagcaaaa agtactgatg atgtcgttga agtaatgaca gctgttgtaa aaggagcaaa
    45541 gcgtgcttta gctaaaactc cagacttgct tccagtccta aaagaagtcg gtgtcgtaga
    45601 cagtggaggg caaggactgc tgtttgttta tgagggcttt ttaagtgcat tgaatggaga
    45661 attccaagcg gatgaaacat atgaaccttc tccagcagaa atggatgata tggtcaatgc
    45721 agaacatcat cgcagtgtcc aaggtcaaat ggcgacagaa gatatccatt ttggttactg
    45781 tacagaaatc atggtaaaaa tcggagaagg acctacagtc gatagtacat tcgattacga
    45841 agaattccgt aactatttag atggtatagg agattcttta cttgtggtca atgatgacga
    45901 gatcatcaaa gtccacgtgc atacagaaaa cccaggagaa gtcttgaatt atggacaaaa
    45961 attcggttca ttagtcaaaa tcaaagtcga caacatgcgc ttgcagcatg agacgatttt
    46021 ggaacacgat gaagaagtga acgagtttgc gaaaaaagaa ataccacgta tcccgtatgg
    46081 tatcgtagcg atcgctgctg gtaaaggcgt ccaagagctg tttgaaagtt taggcgctaa
    46141 ttatgttatc agtggtggac aaacaatgaa tccaagtacg gaagacattg ttaaagcgat
    46201 tcaagaagta aacgcagata aagtgattgt cttgccaaac aataagaaca tcttcatggc
    46261 agctgatcaa gcagcagaag tagcagatat cccagtagca gttgttcctt caagaacgat
    46321 ttcccaagga ttgacagcta tgctttcatt caatgatcaa gcttctttag aagaaaacaa
    46381 agaaacgatg acagaaatgt tgtctagcgt tgtgagcggt caagtcactc acgcgatcag
    46441 agatacctca attgacggtg tagagatcaa agaaggagac tttttgggca tgatcgatgg
    46501 aaaaatcctg atctctaacc cagatatcac agcaacagcg ttaagtacac tagaacaaat
    46561 gatcaatgag gacacagaga tcgtaacgat cttggcaggt gatgaaggaa cagttgaaca
    46621 agcaaatgaa tttgctgaga aactaacaga aaaacatcct gatttggaaa tcgagatcca
    46681 ccaaggagat cagcctgtct atccttatct attttctgct gaataatttc tgtgtcagaa
    46741 tatctttcta acacagtatt cattcataaa aggccttcgg ttagtgatca ggtatgatcg
    46801 ctgaccaagt cctttttttg ttaaaggagg gaggaaaaat ttatgcgttc tttagatgat
    46861 ccaatcacta tgctggcagg agtcggagaa aaaagagcag ctagtcttgc cagcttgggc
    46921 atccaaacga ttgaagactt actgacttat tatccttttc gctacgaaga tattcaggaa
    46981 agaaatctaa acgagatcca ggatcaagaa aaggttactt tgaaaggaat cgtagtctcg
    47041 cctcctgtga tgaatcgatt tggttataaa aaaagccgat tgcaatttcg tatgatgcaa
    47101 gaacatgatg tgttcaacgt ttcttttttt aatcaacctt atctaaaaga aaaagtcatt
    47161 ttatcggagg aaatcgccgt atacggtaaa tgggatgcaa agcgtaaagc cttgaacggg
    47221 atgaagattt taggttctca aacaatggat gacttttcgc cgatatatca cgtcaacaaa
    47281 tcaattcgtc aaacgacatt agttgatcta atcaaaaaag ggtttcaaga atacggcaca
    47341 ttgattgaag aaaatctgcc aaacaacctt gtggagaaat atcgactgct tgaccgagct
    47401 tctgctgtaa gaagtatgca ttttccaaaa gaccacgaag aaaaccatca agccaaacga
    47461 cgtgtcgttt ttgaagagtt ctttttattt caaatgcgtc ttcaaggatt gaaaaaagca
    47521 gaaaaagcag aaacgaatgg tttggagatc ctttatgatg tccaaaaatt gaaacaattt
    47581 actcaaaagc taccttttga actgacaaat gcacaaaaac gtgtaacgaa tgaaatctgt
    47641 cgggatcttc gcagtccgca gcatatgcag cgacttttgc agggagatgt aggtagcgga
    47701 aaaacagtgg ttgccgcgat tgcgttatac gctaccgtta cagcaggttt ccaaggtgcg
    47761 ttgatggtgc ctacagaaat cttggcacaa caacatatgg aaagcttgca acagctgttt
    47821 gatcccaatg aagtaaccat cgcattgatt acggggtcaa cgaaagcaaa agaacgacgt
    47881 gagctccttg aaaaactaga gcaaggggaa attaacgtcg tgatcggcac acatgcattg
    47941 atccaagatg gagtcgaatt tcagaattta ggtctagtta ttacggatga gcagcaccgt
    48001 tttggcgtga accagcggaa agtacttcgt gaaaaaggct ggcgaccaga tgtcttattt
    48061 atgacagcga ctccgattcc tagaacatta gctattacag catatggtga gatggatgta
    48121 tctatcattg atgaacttcc agctggacgt atcccgatcg aaacacgctg ggtaagaaca
    48181 cctcaattgg acagtgtact ggattggacg aaaaaagaac tggcaagagg acatcaaatg
    48241 tatgtcatct gtccgctgat tgaagaatca gaagcattag atgtaaaaaa tgcagtggaa
    48301 atctatgaaa aacttcgtga actatttgca ccaaaatatg aagttggctt gttacatggt
    48361 aaaatgaaga atcaggaaaa agaagtaatc atggaagcct tcaaagacaa ccagatgcag
    48421 atattagttt cgactactgt aattgaagta ggggtaaacg tgccgaacgc gaccgtcatg
    48481 ctgatcatgg atgcggatcg atttggattg gcacagctgc accaacttcg tggacgtgtt
    48541 ggacgcggaa gtgatgcttc ctactgtatc ttagttgcta atcctaaaaa tgaactaggt
    48601 gtggaacgga tgaaaatcat gacagagacc accaatggtt ttgtactaag cgaaaaagat
    48661 ctggaattac gtggtcctgg tgaagtattt ggcttccgtc aatctggtct gcctcaattt
    48721 gcgattgctg acttggtgac ggatggaaat gttttggagg tagccagaga agaggctgca
    48781 gccctttggc aaataaagga ttggcagatg cttcctgagt atcagccttt agcgcaaagc
    48841 ctccaaatag aaaatcagga taatcgttat ttcgattagc agactgtgct atactttata
    48901 ctgatgacgg aaaaaggagt ggaaacaatg aaaattgccg tagatgcaat gggcggagat
    48961 cacgcacctc aagcaattgt agaaggcgtg gctttagcgc agcaagattt tccagaagta
    49021 gaattcttac tttacggaaa agaagcagaa atcaagaaat acttaacaaa tgaaaaaaac
    49081 atcacgatta tccatacaga cgaaaaaatc aatagtgatg atgagccagt aaaagcaatt
    49141 cgacgaaaaa agactgcatc aatggtcctt gctgcacaag cagtgaaaaa cggtgaagca
    49201 gatgctgtat tttcagcagg gaatacaggc gcgctactag cagcagggtt gttcatcgta
    49261 ggacgtatca aaaatatcga acgccctggc ttgatgtcta ccttgccagt agtagggaaa
    49321 gaaggtgccg gtttcgatat gctggatctt ggcgctaacg cagaaaacaa accagaacat
    49381 ttgttgcaat acggtatttt aggttctttt tatgcacgta aagtccgagg tatcggacgt
    49441 cctcgtgtcg cactcttaaa caacggaaca gaagcaacaa aaggtagtga agtgacgaaa
    49501 aaagcctatg aactccttca gaatgaaaca agcttgaatt ttattggaaa cgtagaagct
    49561 cgtgagctac tcaatggtgt ggctgatgta gtggtcacag atggatttac aggcaacgct
    49621 gttttgaaat ctattgaagg aacagcaatg aatatgatga atctattaaa atctgctatt
    49681 ctaaatgaag ggatcaaagg aaaaatgggc gctatgcttt taaaagacgg cttacgttct
    49741 ttgaaagcag aaatggatta ttccaaacat ggcggtgccg ttttgtttgg cttgaaagca
    49801 ccagtcatta aaactcatgg agcaacaggc cccgatgctg ttcgctatac gatccgccaa
    49861 atccacacaa tgcttgagac agatgtagtt ggacaattag tcgaacaatt tgaaaaaagc
    49921 gaagaatagt atttggatat ctgaagcgat ccgttccatt gaaaaaaagg tggacattcc
    49981 tgaaaaatgc cagtaaaatt agccatggtg taggtaattg acaagtgtat gattcttgga
    50041 ggtgaaaaag ttgactcgcg aagaagtatt taacaaagta gcgaaaatta tttcaaatca
    50101 ttttgagata gataccgata aagtgacgga tgaattgaac atcaaagatg atttaaaagc
    50161 tgattcaatt agtattatgg aattcgtttt agagcttgaa gatgaattcg gtacggaaat
    50221 ttcagatgaa gacgctgaac aaattgaaac agtcggtggc gcagtagatt atatcagcag
    50281 ccatcttaaa taatttcaaa taaaagcaaa gaaggataaa aagctgcatg cagcttttta
    50341 tccttctttt tttaatcttt ttgacacgta aaagtcattt ttctgataat tctggcgata
    50401 aacagaaatt atgttgcaaa gtaaagcggt tttctatata attattaata attcattaca
    50461 caaagataag aataaccttt gttataaaac gatttattct gtgtaatgtc cttttttatt
    50521 aagaaaaact tccatttagg aagcagagag gggtattcga ttgtccgata accaattatt
    50581 agaagttcag aacctacata caggattccg actcaaagat gactattacg atgcagtaga
    50641 tgacgtttca tttactttag acaaaaacga gattttagca atcgttgggg aatctgggtg
    50701 cgggaaaagt accttggcta ctacgattat gggattactt gatcctaata acacaaaaat
    50761 tacaggtgag attttatata acgatctgaa tttaatcgac ctgaacgaaa cgctctataa
    50821 taaaatccgc ggaaatgata tcggaatgat tttccaagat ccgttgtctg cattaaatcc
    50881 tctcatgaga atcgaagacc agatcaaaga aggactttcc tatcacacaa agatgaatgc
    50941 agaacagcgt caagctcgtg cactagaatt gctagaacaa gtagggattc ctaatcctgc
    51001 gagagttgga cgccaatatc cccatgaact atcaggcggt atgagacaac gtgtcatcat
    51061 cgcaatcgca atcgcctgca aacctccaat cgtcattgca gatgaaccga cgacagccct
    51121 agatgtaacc atccaagcac agattcttga tttactgaaa gaccttcagg aagaaacaaa
    51181 aacagggatc atcttgatca ctcatgactt aggagtcgta gcagaaatgg ctgatcgagt
    51241 ggcagtgatg tatggcggac agttcgttga agtagcaggt gtaaaagaac tgtttgatca
    51301 tcctaagcat ccttatacac gctctttact gaactctata ccacaagaag gaaatcatga
    51361 tgcagaactc catgtgatcg aaggtgtcgt tccttctctg aaaaacatgc ctcgtaaagg
    51421 ttgtcggttt gcaccacgta ttccatggat accagcaagc gatcatgaag aagagcctat
    51481 ccttcatgaa gtcgcaccga accacttggt acgttgctcc tgctataagc atttccactt
    51541 tagagacgaa aaaggagagg tgtagatgac agaattaatc acaatcaaag acctgaaagt
    51601 tcattaccct attcgcagtg gattcttcaa tcgagtaacg gatcatgtat atgctgtaga
    51661 tggtgtggat tttattatcg aaaaaggaaa gacatatggt ttagtgggag agtctggctc
    51721 tggaaaatct actacgggaa aagccgttgt gggattagaa aaggtaacag ccggacaaat
    51781 catgtatgaa ggaaaagatg taacgaagcg aagcaaccgc aaaaaaatgg gttataacaa
    51841 ggatgtgcag atgatcttcc aagattcaat gtccagtttg aatcctaaaa agcgagtgtt
    51901 ggatatcatt gctgaaccca tccgaaattt tgaacggtta agcgatcaag aagaaaagaa
    51961 aaaggtaaaa ggactgctag atatcgttgg gatgccggaa gatgcgctat ataaatatcc
    52021 tcatgaattt tcaggcggac aacgtcaacg tctaggagta gcccgtgctg ttgctaccaa
    52081 tccaaaactg atcgtagccg atgaacctgt atctgctctg gatctatctg ttcaagcgca
    52141 agtattgaat ttcatgaaac gaatccagca agaatacggc ctcagctatt tattcatttc
    52201 acatgattta ggtgtagtaa aacacatgtg cgacaatatc gcaattatgt acaaaggtcg
    52261 atttgtagaa atcggtacaa gagaagacat ttataatgac ccgcgacata tctatacgaa
    52321 acgtctgctt tcagctattc cgcaaatcga tgtagaaaat agggaaagcc acaaagaaaa
    52381 tcgacgaaga gtagaacaag agtatatcca aaaccaaaaa gaatactatg atgcgactgg
    52441 tcgagtctac gatttgcgta cgatcacaac tacacacaaa gtggcgttga aagatggagg
    52501 tgcaagctaa tgtggaaaac gattctgcga cgtgttttat tgatgatccc gcaagttatt
    52561 attttaagtg ttctgatctt cttattagcc caagcgatgc cgggggatcc atttaccggt
    52621 ttgatcaatc caaatcagga tccggcagtg atcgaacaga tgcgacaagc agctggcttg
    52681 aatgacccat ggtatgtcca gtacttccgt tggatcggaa atgctttgca aggcgatttt
    52741 ggtcaaagtt tcttatacaa acgctctgtt gctgacttga tcggcgaacg gatcatcaac
    52801 actttatatc tatcgatctt gacagttgtc cttacttatt tgattgctgt gcctctgggg
    52861 atgcttgctg gccggtatca aaattcaatc ttggataaga ttgtcgtaat ctacaacttc
    52921 tttagttttg ctgttccttt atttatcttc ggcttgatca tgctatttgt ttttggttat
    52981 cgtttaggct ggttcccaac aagtggatca caaacactag gtttgcaagg tggatttatc
    53041 actcaatgga tagaccgttt gaaatatatt ctgttaccat caattaccca agcatttttg
    53101 gcgactgccg tcacaatcca atatctgcga agtgaagtaa tcgattcgaa atcgctcgat
    53161 tttgtcagaa cagcccgttc caaaggggta ccgacgaata aagtattcag tcgacatatt
    53221 ttccgaaatg cagcgttgcc gatggcttct caaatggggt atgaaatcac ttcgttgatt
    53281 gctggttctg tagttatcga aaagattttt ggttatccgg gagtcggaaa actatttatt
    53341 gattccatcg gccaacggga ctataccgtg atcacatcct tagtattgat tttaggaatc
    53401 gcgacgctag tggggacatt actttcagat atcatcatga gtatcgttga cccgcgtatt
    53461 cggattcaat aaaaccaatc agaaaggaga atggatcgat gagtgaaaaa aacaatacag
    53521 cagttcaaga aagtattcct cctatgggga tacgaatgat tgctcgagaa tttaaaaaag
    53581 ataaaattgc tattttttca ttaggtttgc tagtggtttt attattagtg atctttatcg
    53641 gtgcgatgct gaccgatcaa gacaaagtga tgtatgttag tatcttcgac aaatacgctg
    53701 caccaggaac agtttcgaca caaggacaaa agttcattct gggagcagat gaaggcggac
    53761 gtgatgtatt aggacaattg atcataggtg ctagaaactc tgttacgatc ggatttgcga
    53821 tcacgatcct tacttcgatc atcggggttg gattagggat catctctggc tactacggag
    53881 ggatcattga caacatcttg atgcggatcg ttgattttat catgattcta ccgatcatgc
    53941 tgatcattat cgtcttcgta tcgattattt ccagatacaa tgtctggtcc tttatattca
    54001 tcatgagtgc attttattgg gtagctaaag cccgattatt ccgaagcaag acattatctg
    54061 aagcaagaag agattatgtc agtgcctcaa aaacaatggg aaccaatgat tttaaaatca
    54121 tgttccgcga aatcatgccg aatctaagtt cattaatcat tactaatttg acgatgaact
    54181 ttgcggcaaa catcggtatc gagacaactt tgactttctt aggtttcggc ttgccggcaa
    54241 atgtacctag tttaggtaca ttgatcggtt atgcaagtaa cggagaagtc ttagtgaatc
    54301 ggcaatggat ctggttaccc gcatcaatcc ttattttagt tttgatgttg agtatcaatt
    54361 atgttggaca agcattcaag cgttctgcag atgcgcgcca acggttaggc tagtatagaa
    54421 gcgaaggggg aaataggaat gaagaaaacg atttttggat ttgcagcgct gatgtctgct
    54481 gtcgttcttg cagcatgcgg gggcggaggt acagatacct ctggaaacaa ctctggaggc
    54541 aacggcaata acaaagagga attaactttt ccaattgcaa caaaaaataa tgatgatgca
    54601 atcgaagacg gcgaattaga agctgcggtc gcaacggaca gtcagtttaa aggattgttc
    54661 cagtgggagt tttaccaaga ttcatatgat gcagctttta tggctccttc tcatgaaccg
    54721 ctatttgcaa acgatgacga tttcagaatc acagacgacg gagctgcaac gcttgatcta
    54781 gatgaagata cgaaaaaagc gacaatcaaa gtaaaagaca acgtcaaatg gtcagatggt
    54841 gaagatgtaa cggcggacga tgtgattttc ccttacgaaa tcattgggaa taaagattac
    54901 actggtatcc gttatgacga cactttccgt aacatcgtag gtatggaaga atatcatgat
    54961 ggaagtgccg acacaatctc cggaatcaaa aaaatagacg acaaaacagt tgaaatcgaa
    55021 tataaagaag taaacccaag catgcttcaa tcgggtggtg gtatctggtc atatgcagca
    55081 ccaaaacata cgttagaagg tgtagcaatc aaagatatgg aatcaagcga tcaagtacgt
    55141 aaaaatccag tgacttttgg cccttattac atgagtaata tcgttgcagg agaatcagta
    55201 gaatacttgc caaacgagca ctactggaat ggtacgccta aattgaaaaa gatcacgatg
    55261 aaatctgtac cgatggcaag tgcaactgaa gcattgaact ctaaattata tgatatgatt
    55321 ttccaaatgc caacagatac ttacgatacc tataaagaaa ttgctggcta tacaaactta
    55381 ggccgtgaac aaacttctta tacttatcta ggattcaagt taggtaaatg gaatgcagat
    55441 acaaagagtg tggaatacaa tccagattca aaaatggctg ataaatcatt acgtcaagca
    55501 atgggctatg cattagataa taatgcagta ggtgaacgtt tctatgcagg tttgagaagc
    55561 aatgcgactt cattgattcc tccagtattc cagtcatacc atgatgatga gatgaaagga
    55621 ttcacacaag atctagacaa agcgaaaaaa ctattagatg atgctggcta caaagatgtg
    55681 gatgacgatg gtttaagaga agacaaagat ggaaataaac taacaatcaa ctttgcttcg
    55741 atgtccggtg gggaaacagc gcaaccattg gcagattact atatccaaca atggaaagaa
    55801 attggtttgg atgtccaata cacaacaggt cgtctgatcg aattcaattc tttctatgat
    55861 aaattagaaa acgatgatcc tgaaattgat gtttaccaag ctgcttggaa tacaggaact
    55921 gatccaaatc caagtggact atggggacca aatgctgcgt tcaactacac tcgttatgaa
    55981 tcagaagaaa atacaaaatt gatcaacgat atcagttcaa gcaaagcatt tgatgaagag
    56041 taccaaaaag aagcatatca aaaatggcaa caatatgcat ttgaagaagc atttgcgatt
    56101 ccaacgttgt tccgtaatga agtattgcca gtcaacgacc gtgtcaaaga ctgggattgg
    56161 tcttatgatg taccaaaccc atgggcaaat gtttctgtta cagcagattc acgttcataa
    56221 tcactaaaaa tgaccgaaaa agggcgatgt cctttttcgg ttttttgata tttttctgtt
    56281 tatttctcta atattcaggt caaggcgctg atttcatgac ttctccaata aacggtgttc
    56341 aaagtccagt ttctcggtat taagtcaaat ttatgcaaaa atatggaaag ccattttcac
    56401 aaaatttttt cttcatactc gaaactaaac gaatttttca caacttctcg tcaatcacta
    56461 ggattattaa agctttactt accttttgac cgaaccattt caaatttgac aaaaaaccag
    56521 tataataagg aagtatcgaa aaaatatcca taaaaggaat gtggaaaatg gacaaccagc
    56581 tgacaaaaga actaaaagaa aactataaca tcgtttttca cgatctaaat ttgcttgaac
    56641 aagcttttac ccattcatcc tatgtgaatg agcatcgcaa tctgcaatta tcggataatg
    56701 agcgacttga atttttagga gatgccgttt tagaactgat ggtttctcag tatttattcc
    56761 gtttatatcc tcatttgcca gaaggaaaat tgacaaaaac acgagctgcg atcgttcgcg
    56821 aagacagctt gtcgaaattt gccaaagaat gtcactttga tcaatatgtt ttgttgggca
    56881 agggagaaga gaattcagga ggacgtacgc gaccagcatt gctttgcgac ctattcgaag
    56941 cttttttagg cgcgttatat cttgaccagg gattcgacac gacccatgca ttcatcgaaa
    57001 aagtgatttt tcctaaagtc aaagcaggtg ctttttcaca tgagatggat cataaaacaa
    57061 aactacaaga agtcttgcaa aaatcaggtg atgtatcaat cgaataccga ttgatcaatg
    57121 aagaaggtcc agctcatgaa cgagtatttt ggatcgaagt ttatgtagat gatcaattga
    57181 ttggcactgg acaagggaaa tcgaaaaaat tagcagaaca agctgcggcg gaaaatgcat
    57241 tggcagcact gtaaaagtaa gtgagggaaa gctttgtatt tgaaacgaat tgaaatagcc
    57301 ggttttaaat catttgcaga caagacagtc attgattttg aaaatagcgt cacagcagtg
    57361 gttggaccaa atggcagcgg aaaaagcaat attacagagg ctatccgctg ggtattagga
    57421 gaacaatctg ctaaaagtct gcgaggcgga aaaatgccgg atatcatctt tgccggttcc
    57481 gatacccgta agcagctgaa tattgcagaa gtcacagtga ttttagacaa tagtgatcat
    57541 tatctgccat tggagtacaa tgagatcagt gtaacccgcc gttatcgacg gactggggaa
    57601 agcgagtttt tcatcaataa acaaagctgc agattaaaag atatccaaga attgttctta
    57661 gattctggat taggaaaaga atcgttctca atcatttctc aaggaaaagt agaagcgatt
    57721 ttcagtagta aaccggaaga ccgtcgtggg atctttgaag aagcagctgg tgtcttgaaa
    57781 tacaagcagc ggaaaaagaa agcagaacag aagctttttg aaacagaaga caacctcagc
    57841 cgcgtccaag acatcatcca cgaattggaa gaacagttaa ctcctttggc cgcccagagt
    57901 gaggcagcaa aggagttttt acggctaaaa gaaacactga ctcaaaccga tgtctctttg
    57961 atggttgctg aaatcaagac agcaaaaaaa gattgggaca acaaacaagc acagcttgcg
    58021 aaattcaatc tagaattagg caaactatct gagtcgatcc aagaacaaga atccattttg
    58081 gcgaagcagc gtaaagaaaa tgcgcaagcc gatcgtctga tcgaaaaaaa tcagcaagta
    58141 ttattagatc tttcggaaaa actgaagcag acagaaggac aaaaagatgt tttgcaggag
    58201 cggacaaagc atacacaaaa aagctcgcaa gaatatcaaa cttcattagc agaagcacaa
    58261 aaaaaggtta aacattttga aaagcttcaa gaaagcttga tgaaagcagc agcagaaaaa
    58321 gaaacagaga tccaaaaagc tgaagcaaat ctcataaaaa cacagcaaga attagaaaaa
    58381 taccaaaaat caacgaaaga attattggca gaattaagag atcaatatgt tgatttgatg
    58441 caagaacaag cagctgtagg aaacgaactg aagtatctag aacgtcaata tatacaagaa
    58501 acagcaaaaa gcaagcagac gttagccaag caatcggaag tagaagcatc agtagaccga
    58561 ttgatcttac aaaaagaaga attgacacaa aagcaggcgc agctaaaatc atctttaact
    58621 gaaacaaaag agaagttaga aatgatccaa caaaacggga aaaagttcca agaagcatta
    58681 gcaaaagaac agcctaaaat gtatcaatta atgaatcaag tccagcagct acgtgctcgt
    58741 aagaaaagcc ttcaagaaat ccaagaaaac tattttggtt tttaccaagg ggttcgattg
    58801 gtcttgcagc ataaacagca gctttcgggt attgtcggag cagtggctga actgatcgat
    58861 gttcctgcag atttcacgtt agctatcgaa gcagctttag gtggggcagc ccagcatgtc
    58921 atcgttgaaa atgaaaaaga tgctcggcaa gccatcacgt atttgaaaca gcaaagagga
    58981 ggaagagcaa cattccttcc actgacgaca atcaaaccaa gacaattacc ggctcatata
    59041 ttgacacaag cagcagctgt agaagggttt atagggattg ccagtgaaca agtcagttat
    59101 cccgatcaaa tccaaactgt ggttcaaaac ctgctaggaa cgatcctttt agccaaagac
    59161 ttaactagtg ccaatgcaat cgcgcaaacg atccgctatc aatatcgagt ggtttcctta
    59221 gaaggtgatg tgatgaatgc cggaggatcc atgacaggtg gagcgaataa acgaggaaac
    59281 caaggcagtt tgtttgtaca aaatcaagaa ttgaaacagt tgactagtga atttgaagaa
    59341 gcagataaac aactccaagc acaagaaaaa aaagttcagg agctgcaaca agaaacagct
    59401 cgtttagccg aagaacaaga agttttgcgt acacgaggcg aacaattacg ttttgaagaa
    59461 caagaagcga caaatcagct acaaaacatc ataaatgaac ttgaacgttt tgaaaaagaa
    59521 aagcaaatct caacttttga gacacgtgaa ctgcagcagt ttatcgaaga ttacgaaaag
    59581 caaacgaacg aattgaaaga caaacaaaca gatcttgaat cgcaacgaca aaaaatcgac
    59641 gaagaaatca aatcgttaag tcaagaaagc gaccagatgg aagccagaag agcacaagta
    59701 caaagtcaaa aagcacaaga acaagcagat cttgctgtct tgaaagagca gtttaatcat
    59761 ctgcagatcc agctccgcgg tgcccgagtc caaaaagcag aggcaaccga acgtcaagaa
    59821 gcgatagaaa agcagttggc gacattgaca gcagactttt ctgatcatga agtcacagaa
    59881 gaaagtttgg aaaaacaaat taacgaatta tctgctcagc gagaaacatt aaaagccgag
    59941 ttggcaaaag ccaaagagca gcgagataga aggcaaaagg agatcgataa gcttgaagca
    60001 gtcttggcag agcgtaatcg tgagcaaaaa gcacgactgt cggaacaatc caaattagaa
    60061 gtccaaaaag accgagctga aatgatttta gataatcatt tgacctatct gcagtcagaa
    60121 tatcagctga catttgagaa agcttcccaa gactatcaag aaacaaccga tatcgaagac
    60181 agtagaacaa aggtttcatc cttaaaagaa cagattgaaa aactgggacc agtaaactta
    60241 aattcaatcg aacaatacga acaagtcagt gaaagacata cattcttagc aacacagcga
    60301 gatgatctgt tagcagcgaa aaaccaattg tttgaaacga tggatgagat ggatgatgaa
    60361 gtgcggacaa gattcaaaga agtatttgaa gctatccgtc aagaattcaa aattgtcttt
    60421 ccaaatatgt ttggcggcgg taaagctgaa ttagtactga cggaaccaac tgatttgctg
    60481 aaaacaggta ttgagattga agtacaacct cctggcaaaa aactgcaaag tctaagtttg
    60541 ctttctggag gagaacgagc acttacagcc atcgcgttgc tcttttccat tatccgtgtc
    60601 tgtcctgttc cgttttgtat cttagatgag gtagaagcag cgttagatga agccaacgtg
    60661 aaacgttttg gtcgttactt gagcgatttc caagatgaca cacaatttat cgtagtcaca
    60721 caccgcaaag gaacgatggt cgcagcagat gtactgtacg gagtgacgat gcaggaatcc
    60781 ggtgtatcaa aaatcgtttc tgtacggatg gaagatatca atgaagaggc gaaaatcaac
    60841 gaaacgaaag tgtaggaacg aaaatgatca aattagtagc aatcgatttg gacggtacat
    60901 tattaaatga acaaaagctg atttcagatg agaataaaca agcattagca caagcaaaac
    60961 agcaaggggt caagatcgtt ctatgtacag gacgcccctt agctgcgatg gcgcattatc
    61021 tgcaagaatt aggactggta gatgaaggtg actacagcat cactttcaac ggcggtttgg
    61081 tacaaaaaaa tgatactggt gaaatcatgg aaaagaaagt gatggaagta tcagatatcc
    61141 aacgcttata caaactggct cagcaattgg atctgccact agatgttttg tctgacaatg
    61201 tggttttaca gctgcctagt gccccaagga agcaatcctt gtacaatgtg ttgaataagc
    61261 tgctgcaatt ccaaccagcg gatataagcg atattacaga tgaatttgtg cttaataagg
    61321 cagtaatcgc ttatgaccaa gaagaattag atcctaaaat caaagaaata ccagcagaat
    61381 tccatgaaat gtatgagatc atcaaaacaa gaagtatgct tttggaattt atgccaaaag
    61441 gtgttacaaa agcttatggt atttctttat tggcaaaaga tcttggtttg aaacaagaag
    61501 aaatcatggc gatcggtgat gaggaaaatg atttaccgat gatccaatat gctggactag
    61561 gcgtagcgat ggagaatgct gtgcctttcg tcaaagaagc agcagatttc gtgacagcca
    61621 gcaatgaaga aaatggcgta gccaaagtga tccaaaaatt tgtattagag tagggggagt
    61681 caaatgggat tttttgataa aatcaagcgt gcttttactg gcgaagagaa aacagaagaa
    61741 gttgaagtac aggagaagta cgacaagggt ttagaaaaga cgcgtaaaac atttggtgaa
    61801 cggatgaatg agttgtttgc caattttcga acagtcgatg aagacttttt tgaagaatta
    61861 gaagaaacac ttatcggtgc agatgtaggt tttgaaacag cgatacgaat caccgaagca
    61921 ttacgtcaag aagtgaaact cagaaatgcg aaaaaatcgg cagaagtcca aaatgcgatc
    61981 atcgagaaac ttgtggatct ctatgaagcg gaaggaatca atgaagtaaa tgaactaaat
    62041 atccaagaag acggactaac agttattttg tttgtcggtg ttaatggtgt agggaaaaca
    62101 acaagtattg gtaaacttgc ccatcagttc aaaaacgaag gtaaaaaagt attgatggca
    62161 gcagcagata ccttccgcgc tggagcaatc gaccagttag ttgtctgggg cgaacgcgct
    62221 ggtgttgaag ttgttcgtgg caatgcagga ggcgatccag cagctgtcgt attcgatgct
    62281 ttggaaagag caaagacaga gcaggcagat gttttactag ttgatactgc aggccgactg
    62341 caaaataaag tgaacttgat gaacgaatta gaaaaaataa aacgagtaat ccagcgggaa
    62401 ttaccagaag caccccaaga aacacttctt gtattagatg caaccactgg tcaaaacgcg
    62461 atggttcaag caaaacaatt caaagaaaca acagatgtga ccggtcttgt tttgacaaaa
    62521 ttagatggta cagcaaaggg cggtatcgta cttgctatcc gtaatgaatt gcatttacca
    62581 gtgaaattgg tcggtcttgg tgaaggaatc gatgacttgg agccgtttga tccaaatgat
    62641 tttgtcgttg gattgttcaa aggattacta aaagaagaat aacccatgtc taaaaagcag
    62701 gagctgtgac aaaagttgca gctccttgtt tgaatgattg tttttaacat agctgtgttt
    62761 ttgatgcctt catttttata cttatttgta agcggttctc aaacaatttc gttctttttc
    62821 ttagactttt tgattataat tagattcata tgaattggag gtcacttaat gatgaatttt
    62881 aagcaattat tattgcatgt caatgcgcgc ccttttatcg accaagctcg atttggtata
    62941 gaacgggaag gacagcgggt tgatcttgca ggaaatctag caaaaaccga tcatccagca
    63001 atctttggcg atcgatccta tcacccctat atccaaacag attttagcga aacacaaaca
    63061 gagatgatca cccctgttac cgattctatt cccgaattat ttcagtatct cgctgctgtt
    63121 tatgatgtga ctgctcgttc tataccgaaa gaagagatga tctggccatt aagtatgcca
    63181 cctgccttac cggaaaaaga cgaagagatc attattgcaa aattaaaaaa tttcgaagat
    63241 gtcttgtatc gacgttattt agcaaaagaa tacgggaagc gaaaacagat ggtaagcggt
    63301 attcatttca attttgaatt tggcgatgag ttgctaagaa cattgttcag ccatcaggaa
    63361 gaatttcaag atttttccga attcaaaaca gaactttatt tgaaaacagc gagaaacttt
    63421 atgcgctatc gctggatgat cacttattta ttcggtgctt caccaatgag tgagaagaac
    63481 tactttttag atgaatcaca tccgcaagaa cctgttcgca gtattcgcaa cagtgcgctg
    63541 ggctatacga atcatccaaa tgtgaaagtt tcctatgctt ctatgaaaca gtatttagca
    63601 gatatcgagc gaatgatcga agaagggaaa ctttcggagg agaaagaatt ttatacaccg
    63661 cttcgtttcc gaggcggaaa gaaagtcgca gatttagcaa caacaggtgt tcgttatatt
    63721 gaattgcgga atatcgattt gaatccttat gcaagattgg gtatcaatcc agagcaagtt
    63781 cggttcttac aattattcct gatgtacatg ttatggacag aagaaaaaga agactgtgac
    63841 caatgggtag cagaaggtac gactcgaaat aacaaagtag cactagaaca accatcagat
    63901 caaacagaat ttcatcaaga aggtagagaa attcttgaag gaatgaagca aatgctggtt
    63961 gaattggatt ggttggattc tctttattta gtagaggaag cattgactca aatggatcat
    64021 cctgaacaaa cgttagcagc gaaactctat caagaagcac agctatcgag tcagcaagaa
    64081 gttgctgtgg cattgggaca tcaatattat aaagaaagcc atgaacgtcc ctatcagttg
    64141 gccggttttc gcgaaatgga actttctact cagatattta tgttcgatgc catccaaaaa
    64201 ggtgtccaag tcaaagtatt agatgagtcg gatcaatttt tgcgtctgca attccaagac
    64261 catgtagaat atgtgaaaaa tgcaaacatg acaagtaagg acagctatat cgtcccactt
    64321 atcatggaaa acaaaacagt aacgaaaaaa gtcttaaaag aagctgggtt ccgggtacca
    64381 ggaggcgcag aattttcatc catggaagaa gcggtaaaag cttatccgag atttgccgag
    64441 caggcatttg tgatcaaacc taaatcaacc aattacgggt taggtatcac gattttcaag
    64501 gaaggagcta gtctggaaga ttatcaagca ggtttagcca ttgcttttcg tgaagacagt
    64561 tcggttttag tagaagaatt tatgccggga acggaatatc gtttctttgt gatcgacgga
    64621 gaagtccaag ccatcatgtt gcgagtccct gctaatgtta ttggtgacag tatccgaaca
    64681 gtgaaagaac ttgttgaaga aaaaaatagt gaccctttgc gtggaacgaa tcatcgtgca
    64741 cctttagaat tgatccaatt aggtgaatta gaacaattga tgttgaaaga acaagggttg
    64801 acgatcgaat cagttcccca agccaatcaa attgtttatc taagggagaa ctcaaatatc
    64861 agtacaggtg gtgattcgat cgatatgact gatgaatttt ctgaagctta caaaaaaatc
    64921 gccgtttctg cggtagaagc actaggagct aagattagtg gtatcgattt gattatacca
    64981 gataaagaaa tcgatcctac aactgataaa aaagcatatg ggatcatcga agcaaatttc
    65041 aatccagcca tgcatatgca tgtttatcct tttgcaggaa aaggcagaag attgacgatg
    65101 aatgtattga aattgttata tcctgaagta ttttaaaaga aaaagctttt gacagattgt
    65161 cctatttgat gtcaatctga tcaaaagctt tttttgcatg taaaaattta tgtgtttttt
    65221 cgttttaaaa taaacgatcc cagaagcttt ttttcttttt tggtttttct ttttcagctg
    65281 gcgaagagtt ttcttctggg tatttttttt cgatatcaat cacaggttca tttactgctt
    65341 cttttgctgt taagagcaat ccgaattcct caggatttgc agtgacgtaa tcgttgacta
    65401 cagtgaaacg gctattgttt ttcgttgcta gctggatata ttggttttgg acagaaaggg
    65461 gtagagcccc attcaagagt atggaagcat ctggatgttt tttgagttct tttaaaaagt
    65521 tttctttgtt ttttggttgg atcatttcag caacagtcat actaagatag cagcgttccc
    65581 tgaatgtccc taaatattta tgctgttctt cagggttcac taaaggtgta ccataaagtc
    65641 ccttatccaa atggcttttt acatcatctt gtgccataaa aaataccctc cttcttctct
    65701 ttgtctttct tacgattata gcatattccc aataatacct aaaacatatg aaaaagcggt
    65761 tgcttttgtt caacgattca cttaaaataa gaatgaaaat cattctcaat tagaaaggtg
    65821 gattggatgc gctacggacg actagtagca ttattagtta ttttaagtag tatttcattg
    65881 ttcatcggag tatcgaatgt acaattgtca gaactgctcc agttagatgg gcaaagctta
    65941 caaatcttgc tagtgactcg tttgccacga acgatttgtt tgatccttgt gggagcgacc
    66001 agtagtattt gtgggttgat catgcaacat ttgacccaaa ataaatttgt ttcaccaacg
    66061 actgcgggca cgatggatag tgcgcgttta ggtattttag tggctatgat tttcctacct
    66121 ggtgcatcct tgattgtccg atccttgaca gcattttgct ttgcttttgc aggaacattg
    66181 ctttttttat cccttacgcg attgttcctc cagaaaaatc aattgatgct tccgttgatt
    66241 ggagtcatgt tagggaatat cattggttca gttgctactt tttttgctta tcaattccag
    66301 ttggtacaaa atatgacttc atggcttcaa gggaattttg cgactgtgat acgaggaaat
    66361 tatgagttat tgtatgctgt tgttccattg ttgctcttgg tttggctatt tgcttaccga
    66421 tttacgatca ttggtctggg cgaatcgttt gcagtaaatc tgggaataga ttaccagaag
    66481 atgcagtttt taggcattgg cttagtatca ttggctagtg cagtcatgtt gatcatggta
    66541 ggaagcattc cttttatcgg agtgatcatt ccgaatcttg tctctagaat atatggagat
    66601 caagtgcaca aaacaattgg cgtaactgct ttagcaggta gtttgttctt agtattttgc
    66661 gatcttgtcg ctcgcgtgtt gatttttcct tatgaaatac cagtcagtgt gatcgttgga
    66721 atcatcggtg gggctatctt cctttatctt ctgataaggg gacgacgata tgcttaaaaa
    66781 aaagccatct atttttattg gtatgcttgt tcttgtttta tttgttacag gaatgtactt
    66841 gacgatccat acatatggaa actggcattt tgcatgtgca ttgaggagta agaaaatttt
    66901 tgcatttatc cttgtctcgc tagctgtcag cagtgccaca atcagctttc agacaattac
    66961 gcataaccag tttttgactc cttcagtatt gggacttgag tcgttatatg tccttgtcca
    67021 aacggtcctt ttcttcttta tcggaggagt gacgatgctt cagcagacag gtacagtttt
    67081 tttcttattg aacgttgggc tgatggtcgg gctgagtcta ttgttatttt tctttctttt
    67141 gaaaaaaatg agagcgaatc tttttttatt actgatgtca gggatgattt taggtacgct
    67201 ttttggtagt gtcagtacct ttttacaagt catcatggat ccgaatgaat atgatttgct
    67261 tcaaggcaga ttatttgcaa gctttggcaa tatccagagt cagtatcttt tgcctgcttc
    67321 tttgatcatt atcggctgct ggtacctgtt atttcgatat cgacatgtgc tagacgtttt
    67381 ccatctcgga aatgaacaag cagtaaacct cgggatagac acagatcgtt ttgaattttt
    67441 gattttatgt tgtgtcagtc ttttgacagg gacatcaacg gctcttgtgg gtccagttac
    67501 ttttttaggg tttattgtcg caaatatcag ttatcgtgtc ttttctactt atcgtcatgg
    67561 tcagctattt atcggaagct ttctggtagc tatgctgttt ctgacaggag gacagctcgc
    67621 agtagagcag attttcggct ggaatacaac agtgagtgtc gtcgttgaat ttgtcggagg
    67681 aatttatttt gtcacaaaat tgattcgcga aaggaagagt atcctatgat tgatttacaa
    67741 aaggtcaata aactgtacca tggaaaatca gtggttaaag atgtacagtt aacgattcct
    67801 gaaaagaagt tgactgcttt tatcggtcca aatggtgctg gaaagtcaac ggtgttgtcg
    67861 atgatcagtc gtttgatacc aaaagataca ggtgaaatct atttagacca taacgaagta
    67921 aaggcttgga aatccaatga attagccaaa aaattatcga tcctacgaca atcgaatgaa
    67981 ataaatgtcc aaatcaccgt gaaagaatta gtagaatttg gacgatttcc ttatacgaaa
    68041 ggaaaattga cgacaaaaga cgaggaaatc gttcaggaat cactggaaca ccttgggtta
    68101 gaagaaatag cggatcaatc gattgacact ctttcaggcg gtcaactcca acgtgcgtat
    68161 attgcaatgg tgctagcgca agacacggat tatattttac tggatgaacc tttgaacaat
    68221 ctagatatga attttgcggt tcaaatcatg cagattttaa aaaatttagt acaagaaatc
    68281 gggaaaacaa ttgttattgt tcttcatgat atcaactttg ctgcaagtta tgcagacgaa
    68341 attgttgcaa tgaaagatgg acagttattt acgcaaggaa cgacccaaga aatgataaaa
    68401 aaagatgtat tggatgaatt atacgaaatg gacatacgga tttgtgagat tgaaggccgc
    68461 agattctgtc tttatttcta aaactaacat taggagagaa gagatgaaaa aattagtagt
    68521 aggtatcttg attctagcat catttggttt agcagcatgt ggaaatagca acaacaccag
    68581 tcaggcagac acaaaaaaaa gcagcacaca aactgaaact acattgacga taacagatag
    68641 taatggagac caaatcgagg tgccaaataa tccagaaaaa gtcgtcgtat tcgacaatgg
    68701 ttctttagat acgatggatg ctcttggtgt tggagataag gtagttggag cagctacaag
    68761 cagccttcct gagtatcttt catcctataa aaaagtagaa tcagcaggtg gaataaaaga
    68821 accagactta gaaaaaatta atcagcttca gccagatcta atcatcattt ctggccgaca
    68881 aagagatttt caaaaagatc taagtcaaat tgctccgacc attttccttt ctttagatgc
    68941 gaaaaatcca tgggaatctt tccagcaaaa tgtcactgct ttaggggaga tctttggtaa
    69001 gcaagaagaa gctaaaactc aattagagga attatctagt gcaatcgatc agacgaaaaa
    69061 gaaagcagaa gctactgaca aaaaagcact agtcacactt gtgaatgaag ggcaattatc
    69121 agcctacgga tctggttcta gattcggttt catccatgat ttatttggct ttgaacaagc
    69181 agatgatcaa atcgaagcat ccactcacgg tcaaagtgtt tcttatgaat atgtgctgga
    69241 aaaaaatccg gatatcttat ttgtagtaga cagaacaaaa gctattggtg gagacgacag
    69301 caaagatgat atttctgcca atgaactggt agcacaaacg aatgctggta aaaatcagca
    69361 gatcatctcc cttgaaccag atgtctggta tttaagcggg ggcggtttag aatcaatgaa
    69421 actgatgatc gaagatgtga atcaagcatt taaatagcat aaataacaga ggattaaact
    69481 ctcatactat gtaacaataa tgtttcttac attcttaaat agcaagagcc tatcctcaaa
    69541 gaagttatca aaaaccatcc gtaaaatcgt attgatgatt ttacggatgg tttttattta
    69601 tttgagaaat cttttttcaa cgtgaattca ttatctacat catacgggtt gattctagaa
    69661 tggggagtgg caaaaaatag tgtgatccat tttttcagca tcaagatcgc ctcctttgat
    69721 ttgaatcaaa ggtacaaagc gaaacttaga taaaacttaa atttagatat ttttattttg
    69781 taccgatctc ttctttaaac gatgttccat ctgctgcttc tcggcaataa gtccactaga
    69841 ctagaaaata tgagaagcta ttttctgtcc agcgttctta ttactcaaaa caaatcgcag
    69901 ttgtcacaat ctcttctaaa cgatgttcca tctgctgctt ctcggcaata agcccactag
    69961 actagaaaat atgagaagct attttctgtc cagcgttctt attactcaaa gcaaatcgca
    70021 gttgtcacaa tctcttctaa acaatgttca atcagctgac cttcggtatt agctcaaagc
    70081 actgatttca caacctctat ttaggcatgt gtctcagcaa gcggttgata gttgatgatc
    70141 tgcatttttc gataaaaatc ggcgggaagc ttactccacc gttttagtaa ttgatagaaa
    70201 taactctctt ctgctttgat tttatactcg cttaatatca atccaaaact attcgttttt
    70261 tttgaagcta attttttata aatatccaat gctttggttt tatcttcatt gacaaagagt
    70321 gtttttactt caatataaga accctcgata ttttgtgttt cttcgataaa atagatgttt
    70381 tttatatatt ccttcatagt gattcctcca aagaagtctt ttatttgata agtttactct
    70441 accgaagagt tgttaataat ccgtgaacaa aaagtaattc tttttccaat agttgttaaa
    70501 ggttttttat tttttatagt attttttgtt atttaagaaa ggaataaggt taaacaaata
    70561 acaggttgct ttttgggtct ttttccgttc aagaaactga acgaagaaaa gggaaagttg
    70621 tctcttttgt gataagagaa atcataataa acgaatgact tactttccaa taaattgaga
    70681 acagtcagaa ccagacaaat tagttgcatc tgatgaataa agtgattatt ctatgacatg
    70741 aggtgataca gatgaaatta gaagtgacaa aagaagcaca agaagtattg aagaataaat
    70801 tagaagcaga cgatcaactt ttattagata tcgaaaacgg tgacggtcca tttgcagaaa
    70861 gccaagtcag ttgccaattg gatacggcat ttcgattgat cattgtcaaa aaagatacac
    70921 aaacagatct aagtctttat tctgtagtgg ctgatacacc agttggacca atcaaaatca
    70981 aagacagtgc aatactttac atggatgatc catccacact tgctttagaa ccaacttata
    71041 atagtttaca attaaaagga ccaagcggct tgagaaaagg caacttgcaa gttgttcgaa
    71101 aagactagag ggataaaatg ggaaaattgg cgatcatcag cgacctacat gtggacatca
    71161 ataaattcgg cgaatcagag ctgatgcagc tttggcaagt tttgcaaaag cacaagatca
    71221 ctcggcttca tctagccgga gatacggcta ataaagtaga ccgttgccta gctgttgtcg
    71281 aatttttcgt caataaagga ttatctacga catttcattt cggcaatcac gagttggcag
    71341 atgtgcccaa tgaacagatg atggaccatt ttccagaccc acatttttta aatgaacggt
    71401 atattgcttt aaatgagcag accgttcttt tagggatgaa tggctggtac gactatcagt
    71461 tttccgaatt gaaagattac gaggcaatcg tacgaatgaa gcgtttgtat tggtatgatc
    71521 ggttcatcca gcgtgggaaa acagatctcg aggtcaatgc agaaatgctt gcagctgcaa
    71581 aaaatttgct agaccagctg gaagcaaaaa aacttgaggt gattttatcc actcatttcg
    71641 ttcctaaacg ggcatttatc atgtatcaat ctgctcctta tgagcgttgg aacaaattaa
    71701 atgcattttt aggatcagaa tcattcggag aattacttga ccgttattca aatatcaaac
    71761 aagtagtttt tggccatacg catcgtcgct ttgaagataa atccatcggt cacacgattt
    71821 acagctgcag accttttggt tattattatg aatggcagct gacccgagat tttgtcttga
    71881 aagaacagtt gctagacagc tatgatccta tgaaattacg tgtcctcttg cgtgcccatc
    71941 acccagcttt tcaggcctat caaacacaac atttgcaaga ggaatttgaa caagcgatga
    72001 caatcgttcc gtattgagac agcattagta atgtgtaaaa gaaaatacct tgtaaaacat
    72061 aaggataagt aagagcagta gatccattta tcgtggatat gctgcttttt ttgcggaaaa
    72121 taatcagcga atgtttattt gatcggatga tttgtaactc gcagagtact agatgcaagc
    72181 gtgtattctt tttttgaacg ataatcacaa aaaaaaatat aatatacatt ttttatttaa
    72241 ttataattaa tattgattga agtctaggca gtcaagtcac attttgatag ataggagggg
    72301 gacttacgaa ttattttaaa ggctatgaca gctgtatttc tagattgtgt ttgcatcaag
    72361 ttgcaataaa aactagaaat caaatcgggc tgataatcat ttataactaa tcgatacata
    72421 ggaccaaata taggtctatg ttcttttatt tcataagtat cgtaaaacag aattttgcta
    72481 tggattaatc caatcgactg atatgtttag taaataaaga aagaggaatg tcaatgatga
    72541 aaaaagaaat cgtattaaca gcagcaatga tgttattcag catggtagca agtacgactt
    72601 ttgtttctgc aactgaagta tacccaaagg aatataacac agaaggaacg attacatttg
    72661 aagcaggaga tgagggtgta actcctcctg ttgatccaga gaatcctgat cccaataaac
    72721 ctgtcgatcc atcagatcct ccaagccctg gaacaggagg ggcattatcg attgattatg
    72781 gatcaaaatt caaatttggg actcaaaaaa tttctactgc ggataaaacg tattatgcag
    72841 ctgctgatgt gatgaacgat gggtctagaa aaccgacata tgtccaagtg acagatagga
    72901 gaagtacctt atctgggtgg aaattaagtg tctctcaacc agaacagttt aaaactgcca
    72961 gtggggatga actagttgga gcacagctga aatttacgaa agggcaggct gtttctttgg
    73021 ttgatccaac atatacgccg caaacggtga attctgaatt aacgttgaca ccgggaggaa
    73081 acaatacgtt agcaattaat gctaaatcag gaacaggtgt ggggacttgg gtctatcgat
    73141 ttggtgcaaa tgaaaatgaa aatcaagatg cggtacagct ttcggtaccc ggtaaatctg
    73201 tcaagttagc gcaacaatat tccactaaat tagtttggac attagaagat acacctaaca
    73261 actagtttac tgaaaagaac tgttaaaaat aaatacgtag tcatattgtc ttgcgaggta
    73321 atcgagcaaa aattgtcaaa ggctaggcta aatttgtttg gatacgaata gttcatctag
    73381 gaatttaacg atcaggagga aaagatatat tgaaaaagag gaggatcgtg ctcattttaa
    73441 gcagtctctt agtgactaat atctttccga tcatagggta ctctttggaa ttgaatcagt
    73501 tggagcatgg aaatagaaga gacgaagaaa gcaaactcac ttcgacattc gacagtttaa
    73561 tagaatccaa ccagacacaa gaaagcagtg aggatttaag tatttcgagt agtggagaga
    73621 ccactgacaa cgtagaagct gagggttcgg acaaacaagg actagaagat gcaccaattt
    73681 ctgcggttga gctgcctgac gatcccaata ttatttctat cgataaaatt tttcaaacac
    73741 ctatcggtac tagcacgtcc atattggaaa atgggaagtt actgcagttg aacccagcag
    73801 aaaggtctca aagaggagct atttggtcaa ataagaagat tagtttactc tcggatttta
    73861 catttaaatc ttatttatac ttaggaaatc aacgagatag agcgggagat ggcatgacct
    73921 ttacattgac tggcgatcct agaatggcta gtaatcccag tcaagttatt ggatcgccag
    73981 gtatgggaat tggctcatat tctactcgtt ctggccaacc ttacattgcg aatgcgctct
    74041 cgattgagtt tgatacgtat aaaaatagtg gttcttctga tcgaatggat cgagagataa
    74101 ctgctgataa tagagggcat ggacatgtcg cttttgtcac gcctaaacca aataataata
    74161 attattctgg agagcatact ggcgtgacgg tagctcctac atttctttct aatggcacat
    74221 ggcgcttgtt aataattaaa tgggacgcgg agcaaagaag tcttacttat gatttggaag
    74281 gtgtcggtcg ctcttctcgg attatacctg atctcattag gcagtttggt tctacagaag
    74341 taaattgggg gtttacttct tcaacaggta ctttttttca agaaaatgct gtcgctgtca
    74401 cgcgacttcc atcgaatgtc acatcaacag ctgatgtaaa agtcaacgat agtgattatg
    74461 gggccatcgc tgaagctgga ggaaacgata agataactat cagaaataca ttgaaggtcg
    74521 agggggattt tcttgaatat aagaaaatgc agactagtat cgacctccca caagaattaa
    74581 ctattcccga gcatatcttg atcaatggaa atcaaattga tgccaaagat atcttgataa
    74641 ccggaaatcg agtcacgcta aatctgacag attattttat tgctggagat acgttaataa
    74701 tcgaattgga gacaactttg caggaagtaa ttcctgaaaa agtactcact atgcattttg
    74761 aataccttga aaacgggaat ttgattgagc aatcgaatga aataaaccta aagatcgcaa
    74821 agctgaagga gatagatatc catgtttatt ataaagataa agacagtcag attgatattg
    74881 ctgaaatgaa aacaatcact gggaaaatag gagagaagta taaagaaact cctattgata
    74941 ttgaagggta tgtttttgac tcggattcag cgaatacaga aggcatcttc tccgaaaata
    75001 cagaggatat ctatttttat tacagaaaag gcaaacttta tctctcaact gctcctcaat
    75061 atgtcaattt tggaaaacat aaaatatcca gtagaccatt aagtgaattc ggacagtttt
    75121 caggtgggtt gaaaattata gatgaacggg cttttggaag ttggaaatta caattgaaac
    75181 aagagaaagt tcttacaaat ggagattttg agcttccaga agctctttcc tttgttacct
    75241 ctgaaaatac aacggttata gggacatccg ctgttacgat tttcgaaagt gatcaaaagg
    75301 gtgaaagcga tttgtcctct cttttagatt ctacagctca tcgtggaatc agaataaata
    75361 ttcctgtaga aaaccaaaga atcggaacct ttgaggggaa actatcttgg atacttgcag
    75421 atgtcccagg aaattaacag gaagagaaga atataacaaa attattgagt ttcaagaagg
    75481 gcacaggtac cataggctct tgtgtcccac agagacaaga ttttgttata gtcttctctt
    75541 acatatgatt aaaggagcga ttttcttgag accaaaacaa caattattta taacactcct
    75601 agttatttta ggggcacttt ttaacaatat atcagtgtca gctagtgcat ttaatttttc
    75661 agtgacgccg ataacttcag agaatcaaat cgacaagaga aaaacttact tcgatcttca
    75721 acttgatccg gatcaggaag tagaagtaaa agcggaattg agaaatgata cagaaaaaga
    75781 agtgaaaatc gatatttctg tgaatagcgc aaccaccaat tcaaatgtga tggttgaata
    75841 cggaaaaaac gagattgaaa aagacatgag cctgatcttt gatctggtag attatgtgag
    75901 ctatccacag atagtgacgc taaaacctaa aagtgtccaa accgttgctt ttcatgtcag
    75961 gatgccgaat gagcgatttg atggtgtatt ggctggtggg atcacattcc aagaaatcca
    76021 gacggaaaag gaccaaacag aaactaagga tcaaagctta tcgattgaga atgaatacac
    76081 ctatattatt gctttgttga tgcaacagac actgaacgaa gttgctccga accttactct
    76141 tcatgaagtt aaaccagatc aaatcaatgc aagaaatgtc atcttagcaa acgttcaaaa
    76201 tgatcaaaaa acctatatca atcaagtagt tattgaaaca aaaattacaa aaaaaggaca
    76261 ttctgaagtt ttatatcaag aggaaaaaga aggattgcag attgcgccga atacaaattt
    76321 ttcttttcca actgctttaa atggtcaacc gctaacccca ggtgaatatc accttacaat
    76381 gactgtttta ggaaatgaaa atgaaaatgg aaaattttcg agaaaaaaag gaaatactac
    76441 gatcaactat accaatcaat gggtatttga aaaagatttt acaatagacg gaaaagtagc
    76501 caaggaattg aacacgacgg atgttacgat taaaaaagac aataattgga tttatatctt
    76561 agtagcttca cttttctt
//
LOCUS       DACDPS010000005        66096 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.005, whole genome shotgun sequence.
ACCESSION   DACDPS010000005 DACDPS010000000
VERSION     DACDPS010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 66096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66096
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.005"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            172..900
                     /locus_tag="IWJ07_000376"
     CDS             172..900
                     /locus_tag="IWJ07_000376"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAP9331984.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            900..1154
                     /gene="purS"
                     /locus_tag="IWJ07_000377"
     CDS             900..1154
                     /gene="purS"
                     /locus_tag="IWJ07_000377"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAP9331985.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1156..1827
                     /gene="purQ"
                     /locus_tag="IWJ07_000378"
     CDS             1156..1827
                     /gene="purQ"
                     /locus_tag="IWJ07_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAP9331986.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            1828..4050
                     /gene="purL"
                     /locus_tag="IWJ07_000379"
     CDS             1828..4050
                     /gene="purL"
                     /locus_tag="IWJ07_000379"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAP9331987.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4035..5474
                     /locus_tag="IWJ07_000380"
     CDS             4035..5474
                     /locus_tag="IWJ07_000380"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAP9331988.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            5506..6549
                     /locus_tag="IWJ07_000381"
     CDS             5506..6549
                     /locus_tag="IWJ07_000381"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAP9331989.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            6546..7124
                     /gene="purN"
                     /locus_tag="IWJ07_000382"
     CDS             6546..7124
                     /gene="purN"
                     /locus_tag="IWJ07_000382"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAP9331990.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7121..8656
                     /gene="purH"
                     /locus_tag="IWJ07_000383"
     CDS             7121..8656
                     /gene="purH"
                     /locus_tag="IWJ07_000383"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAP9331991.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            8672..9946
                     /gene="purD"
                     /locus_tag="IWJ07_000384"
     CDS             8672..9946
                     /gene="purD"
                     /locus_tag="IWJ07_000384"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAP9331992.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10068..10982
                     /locus_tag="IWJ07_000385"
     CDS             10068..10982
                     /locus_tag="IWJ07_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAP9331993.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11115..12680)
                     /locus_tag="IWJ07_000386"
     CDS             complement(11115..12680)
                     /locus_tag="IWJ07_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP9331994.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(12673..14124)
                     /locus_tag="IWJ07_000387"
     CDS             complement(12673..14124)
                     /locus_tag="IWJ07_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9331995.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14138..15154)
                     /locus_tag="IWJ07_000388"
     CDS             complement(14138..15154)
                     /locus_tag="IWJ07_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9331996.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15374..16462
                     /locus_tag="IWJ07_000389"
     CDS             15374..16462
                     /locus_tag="IWJ07_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP9331997.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            16516..18042
                     /locus_tag="IWJ07_000390"
     CDS             16516..18042
                     /locus_tag="IWJ07_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAP9331998.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18044..19249
                     /locus_tag="IWJ07_000391"
     CDS             18044..19249
                     /locus_tag="IWJ07_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9331999.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            19506..20483
                     /gene="guaC"
                     /locus_tag="IWJ07_000392"
     CDS             19506..20483
                     /gene="guaC"
                     /locus_tag="IWJ07_000392"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAP9332000.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            20747..22171
                     /locus_tag="IWJ07_000393"
     CDS             20747..22171
                     /locus_tag="IWJ07_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP9332001.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            22632..23741
                     /gene="pdhA"
                     /locus_tag="IWJ07_000394"
     CDS             22632..23741
                     /gene="pdhA"
                     /locus_tag="IWJ07_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAP9332002.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            23744..24721
                     /locus_tag="IWJ07_000395"
     CDS             23744..24721
                     /locus_tag="IWJ07_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAP9332003.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            24746..26389
                     /locus_tag="IWJ07_000396"
     CDS             24746..26389
                     /locus_tag="IWJ07_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAP9332004.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSASSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            26397..27803
                     /gene="lpdA"
                     /locus_tag="IWJ07_000397"
     CDS             26397..27803
                     /gene="lpdA"
                     /locus_tag="IWJ07_000397"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAP9332005.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            27965..28240
                     /locus_tag="IWJ07_000398"
     CDS             27965..28240
                     /locus_tag="IWJ07_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAP9332006.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            28246..29013
                     /locus_tag="IWJ07_000399"
     CDS             28246..29013
                     /locus_tag="IWJ07_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAP9332007.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            29181..31010
                     /gene="typA"
                     /locus_tag="IWJ07_000400"
     CDS             29181..31010
                     /gene="typA"
                     /locus_tag="IWJ07_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAP9332008.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            31271..33043
                     /gene="recQ"
                     /locus_tag="IWJ07_000401"
     CDS             31271..33043
                     /gene="recQ"
                     /locus_tag="IWJ07_000401"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAP9332009.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            33157..33459
                     /locus_tag="IWJ07_000402"
     CDS             33157..33459
                     /locus_tag="IWJ07_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAP9332010.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            33462..34625
                     /locus_tag="IWJ07_000403"
     CDS             33462..34625
                     /locus_tag="IWJ07_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP9332011.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            34651..38079
                     /locus_tag="IWJ07_000404"
     CDS             34651..38079
                     /locus_tag="IWJ07_000404"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAP9332012.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            38100..39224
                     /locus_tag="IWJ07_000405"
     CDS             38100..39224
                     /locus_tag="IWJ07_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332013.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            39214..39651
                     /locus_tag="IWJ07_000406"
     CDS             39214..39651
                     /locus_tag="IWJ07_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP9332014.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            39635..39973
                     /locus_tag="IWJ07_000407"
     CDS             39635..39973
                     /locus_tag="IWJ07_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAP9332015.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            40092..40652
                     /gene="rsmD"
                     /locus_tag="IWJ07_000408"
     CDS             40092..40652
                     /gene="rsmD"
                     /locus_tag="IWJ07_000408"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAP9332016.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            40649..41140
                     /gene="coaD"
                     /locus_tag="IWJ07_000409"
     CDS             40649..41140
                     /gene="coaD"
                     /locus_tag="IWJ07_000409"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAP9332017.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            41130..42173
                     /locus_tag="IWJ07_000410"
     CDS             41130..42173
                     /locus_tag="IWJ07_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP9332018.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            42332..43060
                     /locus_tag="IWJ07_000411"
     CDS             42332..43060
                     /locus_tag="IWJ07_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAP9332019.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            43176..43421
                     /locus_tag="IWJ07_000412"
     CDS             43176..43421
                     /locus_tag="IWJ07_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP9332020.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            43418..43636
                     /locus_tag="IWJ07_000413"
     CDS             43418..43636
                     /locus_tag="IWJ07_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAP9332021.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            43772..44311
                     /locus_tag="IWJ07_000414"
     CDS             43772..44311
                     /locus_tag="IWJ07_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAP9332022.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            44304..46577
                     /locus_tag="IWJ07_000415"
     CDS             44304..46577
                     /locus_tag="IWJ07_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAP9332023.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            46615..47661
                     /gene="holA"
                     /locus_tag="IWJ07_000416"
     CDS             46615..47661
                     /gene="holA"
                     /locus_tag="IWJ07_000416"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAP9332024.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(47750..48004)
                     /locus_tag="IWJ07_000417"
     CDS             complement(47750..48004)
                     /locus_tag="IWJ07_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAP9332025.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(48404..48673)
                     /locus_tag="IWJ07_000418"
     CDS             complement(48404..48673)
                     /locus_tag="IWJ07_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332026.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(49143..49598)
                     /locus_tag="IWJ07_000419"
     CDS             complement(49143..49598)
                     /locus_tag="IWJ07_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAP9332027.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(49736..51664)
                     /locus_tag="IWJ07_000420"
     CDS             complement(49736..51664)
                     /locus_tag="IWJ07_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP9332028.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(51861..52694)
                     /locus_tag="IWJ07_000421"
     CDS             complement(51861..52694)
                     /locus_tag="IWJ07_000421"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAP9332029.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(52886..53590)
                     /locus_tag="IWJ07_000422"
     CDS             complement(52886..53590)
                     /locus_tag="IWJ07_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9332030.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(53613..54239)
                     /locus_tag="IWJ07_000423"
     CDS             complement(53613..54239)
                     /locus_tag="IWJ07_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP9332031.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            54565..56940
                     /locus_tag="IWJ07_000424"
     CDS             54565..56940
                     /locus_tag="IWJ07_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAP9332032.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(56964..57455)
                     /locus_tag="IWJ07_000425"
     CDS             complement(56964..57455)
                     /locus_tag="IWJ07_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAP9332033.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            57642..58562
                     /locus_tag="IWJ07_000426"
     CDS             57642..58562
                     /locus_tag="IWJ07_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9332034.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            58716..59060
                     /locus_tag="IWJ07_000427"
     CDS             58716..59060
                     /locus_tag="IWJ07_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAP9332035.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(59250..59750)
                     /locus_tag="IWJ07_000428"
     CDS             complement(59250..59750)
                     /locus_tag="IWJ07_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9332036.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            60278..60361
                     /locus_tag="IWJ07_000429"
     tRNA            60278..60361
                     /locus_tag="IWJ07_000429"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:60312..60314,aa:Leu,seq:cag)
     gene            complement(60685..61323)
                     /locus_tag="IWJ07_000430"
     CDS             complement(60685..61323)
                     /locus_tag="IWJ07_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9332037.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            61658..62092
                     /locus_tag="IWJ07_000431"
     CDS             61658..62092
                     /locus_tag="IWJ07_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9332038.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            62115..62435
                     /locus_tag="IWJ07_000432"
     CDS             62115..62435
                     /locus_tag="IWJ07_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332039.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            62576..62767
                     /locus_tag="IWJ07_000433"
     CDS             62576..62767
                     /locus_tag="IWJ07_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332040.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(62838..63095)
                     /locus_tag="IWJ07_000434"
     CDS             complement(62838..63095)
                     /locus_tag="IWJ07_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAP9332041.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            63253..63516
                     /locus_tag="IWJ07_000435"
     CDS             63253..63516
                     /locus_tag="IWJ07_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332042.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            63540..63728
                     /locus_tag="IWJ07_000436"
     CDS             63540..63728
                     /locus_tag="IWJ07_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332043.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(63811..63996)
                     /locus_tag="IWJ07_000437"
     CDS             complement(63811..63996)
                     /locus_tag="IWJ07_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332044.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            64211..64582
                     /locus_tag="IWJ07_000438"
     CDS             64211..64582
                     /locus_tag="IWJ07_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9332045.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(64732..64804)
                     /locus_tag="IWJ07_000439"
     tRNA            complement(64732..64804)
                     /locus_tag="IWJ07_000439"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(64769..64771),aa:Gln,seq:ctg)
     gene            complement(64850..65278)
                     /locus_tag="IWJ07_000440"
     CDS             complement(64850..65278)
                     /locus_tag="IWJ07_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332046.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(65338..66096)
                     /locus_tag="IWJ07_000441"
     CDS             complement(65338..66096)
                     /locus_tag="IWJ07_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAP9332047.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT        21803 a        11333 c        14091 g        18869 t
ORIGIN      
        1 gatggtataa gattttcgtt tctgataaaa cacgaacatt aaaagaatct aatgccttaa
       61 atgtacatat ttttgttgat ttataaaaag atgattgcta aaatttaaag tgaaaaaacg
      121 aacaaaaaac gatgtatttg cttttaatgt aaataaaaag gagagacgta catggagaag
      181 aaagaacttt tgtatgaagg aaaagccaaa aaaatatatc ttacttctga tgagaacttg
      241 atttggatag aatatttaga tcaagcaact gcgttgaatg gcgcgaggaa agaccagata
      301 agtggaaaag gagaattgaa taatcaaatc acaagtgcaa ttttccgtta tctgactcaa
      361 agaggaataa aaaatcattt tattgaacaa ttgtctaaaa ccgaacaatt agcaaaaaaa
      421 gtacaaatta tccctctgga agtggttatc cgaaatattg cagcaggaag tttctcaaaa
      481 cgtctaggtg ttcccgaagg taaagaattc atcaaaccaa tcattgaatt ctactataag
      541 gatgaccagt tggatgatcc atttatcaat gaagagcata tctatttctt acaacttgca
      601 aaaccggaag aaatcagtga gctgaaagcc caagcgttgg ctattaatga aattttgacc
      661 gaattatttg ctaaggtcgg tttggtttta gtagatttca agctagaatt tggccggtcg
      721 aatggacaaa ttttgcttgc ggatgaagta acaccggata cttgccgatt atgggatgaa
      781 cgtacgaatg aacatatgga caaagacgtt taccgcagag atctaggtga tttgatccca
      841 gtctatcaag aagtatataa ccgtctatcg gaactaactg aagaaaagga gcgagtatga
      901 tgtatttcgt aaaagtatat gtaacctata aagattcagt gttagatcct caaggggagg
      961 cagtaaaaaa agcagtccat cgtatgggat atgaaacaat cgaagatatc cgaataggaa
     1021 aatactttga gatacaactt gctgatgcag aagaaccaat cgatacagtg attgaaacga
     1081 tttgtgacaa gctgcttgca aatgtaaata tggaaacata ccgatatgaa atcaaaaaaa
     1141 tggaggaagt ataacatgaa atttgcggtc atcgtttttc caggttcaaa ttgtgacgca
     1201 gatatgttat gggcagttcg cgagatcatg ggcgctgatg ccgaatttgt acggcatgat
     1261 gagtcctctt tagaaggatt tgatggggtt ttactgccgg gaggattctc ctatggtgac
     1321 tatttgagat gcggtgcgat tgctcggttt tctacaatca tggaagaagt gattcgtttt
     1381 gctgaagaag ggaaaccagt attcggtaca tgcaatggat tccagatatt gacagaagca
     1441 ggacttttgc caggtgtact gagacgaaat gagtctttgc attttatttg caaaactgta
     1501 ccattgaaag tcaaccccaa gacggccttt acttcagagt atgaagccga cgaaataatc
     1561 tatcttccag ttgctcatgg ggaaggaaat tatttctgtg acgaagaaac acttcaagaa
     1621 ctaaaaaaaa ataatcagat tgcctttact tataatgaca acatcaacgg cagcgtggaa
     1681 aacattgcag ggattacgaa caaagcaggg aatgtgttag gaatgatgcc tcatccagaa
     1741 cgtgcagtag aagacttgct aggatcagaa gatgggaaac ggttctttgc ttccatttta
     1801 aaaaacttcg gaaaggtgac cgtatagatg aaaacattga aagaaccgac agctgaagag
     1861 atcaagaaaa aaagaatcta tgccgaatgg ggattgactg atgaagaata tcgaatgatc
     1921 gaagaagata tcttaggaag aatgcctaat tatacggaaa ctggactgtt ttctgttatg
     1981 tggagcgaac attgttccta taaaaattct aaaccggttc tacgcaaatt tcctactagt
     2041 ggacctcatg tgttgcaagg ccctggagaa ggcgcaggca tcgtcgacat cggtgatgga
     2101 ctagcagttg tcttcaaagc agaaagtcac aatcatcctt cagccgtgga gccttatgaa
     2161 ggagctgcga caggagttgg cggaatcatc agagatattt tcagcatggg ggctcgtcca
     2221 attgcgttgc tagattcatt gaggttcggc gaattagata atccaagaac gaaatattta
     2281 ctagaagaag tagttgcagg tatcggcgga tacggcaatt gtatcggcat accgactgta
     2341 ggcggcgaaa ttgcatttga tccttgttat gaaggaaacc cattagtcaa tgcaatgtgc
     2401 gtaggtttga ttgaacacaa acatatccag aaagggcaag ccgctggcgt cggtaattcg
     2461 atcatgtatg taggtgcaaa aacaggtcgc gacggtatcc acggagcaac ttttgcctca
     2521 gaagaattta atcaagaaga agaacagcaa cgttctgctg tacaagtagg cgatcctttt
     2581 atggaaaagc tgctgcttga agcgtgtttg gaattaatct tgcatcattc ggattctcta
     2641 ataggtatac aggatatggg agcagcagga cttgtttcat ctagtgcgga aatggcttca
     2701 aaagctggct caggacttat attgacatta gatgaagtcc cacaaagaga aactgggatg
     2761 acaccttatg aaatgatgtt atcggaatca caagaacgga tgttgatttg tgttaaatca
     2821 ggagaggaag aaaaaatcca agaactattt cagaagtatg atctagatgc agtcactatc
     2881 ggtaaagtaa cagacgacgg gcaatatcgg ctatatcatc aaggagaaga agtagcaaat
     2941 ctgccagtcg atgcattagc tgaagatgca ccagtttatc ataaagaaat gaaagaacca
     3001 gctcgaatcg tagaatttca gcagttagct ccttatcagc cagttatcga agaacctgga
     3061 caagtattgc ttgatctgct gcagcaaccg acgattgctt caaaacgttc aatctacgaa
     3121 acttatgatt cacaagtaca aaccaataca gttgtccgtc caggaagcga tgctgctgtg
     3181 atgagggtta gagggacgaa taaagcgtta gctatgacca cagactgcaa cgcgcgctat
     3241 ctgtatttgg atccaaaaat cggaggacaa atagcagtgg cagaagctgc tagaaatatt
     3301 attgctagcg gtggtaagcc cttggcgatc accgactgcc tgaactatgg ttctcctgat
     3361 aaaccagaag ttttttggga attatcgact tcagctgacg gaattgctgc tgcgtgtgaa
     3421 acgttaggaa ctcctgttat ttcaggaaat gtctctttat ataatgaaac ggatggtcaa
     3481 gcaatctatc caacaccaat gattggtatg gtcggattga ttgaggatca taaacatatc
     3541 actactcagg aattcaaaaa atcaggggat ctaatctata tattaggtaa gacgtttgca
     3601 gattttgatg gcagtgaatt acaaaagatg cagcttggaa gaatcgaagg tgtgatccga
     3661 aactttgatc tatccattga aaagagaaac caagagttag tattgacagc catccaaaac
     3721 ggattgattg aaagtgctca tgactgttca gaaggtggct tggcaattgc actggcagaa
     3781 tcagctttca aacatcagct agggatatct gtacagtttg aattgtcctc tgctcaatta
     3841 tttgcagaaa ctcaatcaag atttgtgttg acagtcgcac cggaaaataa gacacgattc
     3901 gaagaaatga tgggagatgc tgctgtttta gcaggtaagg tgacggatga agcgataatc
     3961 gaaatttcag caactgacgg tcagatcaaa atagaaactg cagtagcaag aaagtgctgg
     4021 gaggatgcaa tcgtatgtct tatgaagtga agagtttaaa tgaagaatgc gggatcttcg
     4081 gtgtttgggg tcatccagac gccgctagtg tggcatactt cgggctccat agtcttcagc
     4141 atcgaggaca agaaggtgca ggtatcgtgt ccaatgatcg aggaaagctt aaggggcatc
     4201 gagatttggg gctgctttct gaggtattca aagatcaaag aaaattgcag cagttaacag
     4261 gagaagctgc aatcggacat gtacgttatg caacagcagg caatggaagt gtggacaata
     4321 ttcagccttt tctattcaaa tttcatgatc agtcgatcgg cttagcgcat aatggtaact
     4381 tgatcaatgc tcgttccctt agaaaagagt tagaggaaaa tggggcgatc ttccattcta
     4441 actccgatac ggagatattg atgcatttga tccgacgaag tgaagaaaca gattttttaa
     4501 ataaattaaa aagtgctttg aatgaagtca aaggtggctt tgcctattta ttaatgacag
     4561 aaacggcaat gattgccgct ttggacccaa atggttttcg tccattagca atcggacaaa
     4621 tgaaaaacgg agcatacgtg attgcctcgg aaacttgtgc attagaagtt attggggcac
     4681 gttttataag agatgtccag cctggtgaga tagtgatcat caatgacgaa ggaatccaga
     4741 ttgattgctt tacgaaggac acgcagcttt ctatttgttc gatggaatat atctattttg
     4801 ccagaccaga ttcaaacatc gcagggatca atgtgcatac tgcacggaaa aatatgggac
     4861 ggaatttagc gaaagaatcg ccagtaaaag cagatatggt cgttggcgta cctaattctt
     4921 ctttgtcagc cgcaagcggt tatgcggaag ctagtggaat cccttatgaa atcggattag
     4981 taaaaaatca atacattgcc cgaacattta tccagccaac gcctgagcta agagagcaag
     5041 gtgttcgaat gaaattatct gctgtacgag gagtagtgga aggtaaaaag gtgattatgg
     5101 tcgatgattc aattgttcgg ggtacgacaa gcagacggat cgtccatctt ttgaaagaag
     5161 caggggcaaa agaagtccat gtgaggattg cttctcctcc tttgaaatat ccatgttttt
     5221 acgggattga tattcaaacg cgtaaagaac tcattgcggc aaatcacacg attgatgaaa
     5281 tcaaagaatg tatcggtgca gattctttag gttttttaag tgaagagggt ctgatcgaat
     5341 cgatcggtct ggaaaaagat gctccgtatt caggattgtg tatggcttat ttcaacggag
     5401 attatccgac gccgttatat gactatgaag aaaaatatca ggaatcacta aaagaaaaag
     5461 tatcgttctt ttaaaagcag accaagaagg gagtatatga attcaatggc aaatgcatac
     5521 gcaaaagctg gcgtagatgt cgaagctgga tatgaagtag tagaacggat caagaagcat
     5581 gtcaaaaaaa cggaacgttt aggtatgatg ggcgctatag gaggattcgg cggttgtttt
     5641 gatttatcgg tctttcaatt aaaagagcca gtccttgtct cggggaccga tggcgtgggg
     5701 acgaaattag cagtggcaat cgaagcagac aaacacgata caatcgggat cgactgcgta
     5761 gcgatgtgtg tcaatgatat cttagcacaa ggcgcagagc cgttatattt cttagactac
     5821 ttagcaacgg ggaaaaatga tccgttacgt ttggaaaatg tagtaaaagg tgtagcagat
     5881 ggatgtgttc aaggaaatat ggcgctgatt ggcggagaaa cagctgaaat gcctggtatg
     5941 tactcaggta atgattatga tctagccgga tttgctgtag ggatagctga aaaatctcaa
     6001 ttattgacag gagaaacgat caacccagga gatgtgttgc ttggaatagc atctagcggg
     6061 attcattcaa atgggtactc tcttgttcgc aaaattttct ttgagcagca gcagttttct
     6121 ttatcatcaa aactgccaga attgaaaaaa acacttggcg aagaattgct tgagccaacg
     6181 aaaatctatg tttcttcctt gcttccgctg atcaaagaaa agaaaataaa aggagctgca
     6241 catatcactg gtggagggtt tgtagaaaat atcccgcgaa tgttaccaga atctatggct
     6301 gcacaaatca aacttggctc atggccgatt cttccaatat tcggctgttt acaaaaatat
     6361 ggtgaaattc ctgagatgga gatgtatgaa atcttcaata tgggcatcgg tatggtcctg
     6421 gcagtcgaag aatcgaaagt aaaagaaata gtacacgaaa ttgagcaagc tggcgaaaaa
     6481 gtttatgaaa tcgggaaagt gatcgccaaa gaaaaacatc gtgtggtgtt tataggagaa
     6541 gaacgatgag aatcgctgtt tttgcttctg gaaacggaag caactttcaa gcgctagccg
     6601 attatctatc gaaaaaagga ttggaatctt cgattgactg gttgttttgt gatcagcctg
     6661 aagcatatgt tttgaaacgt gcaactgcgt tgagtgtacc ggcagattgt ttttcaccta
     6721 aagaatttga ttcaaaaaaa gagtatgaag aagcaatttt gcataaattg aaagaaaaaa
     6781 agatcgattt gatcgtatta gctggatata tgcgaatcat tggaccggta ttgctgaaaa
     6841 attatgataa aagaatcatc aatatccatc cgtctctttt acctgccttc cctggtttgc
     6901 atggcatacg tgatgccttt gaagctggag taaaagaaac gggtgtcaca attcattata
     6961 tcgaccaagg tgtagatact ggaccaatca tccggcagga aaaagtgaga attgaacagg
     7021 aggacacgtt cgattcttta gaagaaaaaa tccatagagt agagcatcga atctatccag
     7081 aagtgattag tgaaattatt gaaaatggag gctatctgaa atgacaagag cattgatcag
     7141 tgtatcagat aaaactggga ttgtagcatt cgcccaaggt ttgattgaac aaggagtaga
     7201 aatcatttca acaggaggaa caaagcatgc gttggataaa gctgggatca aaacgctttc
     7261 gatcgaagaa gtgacagggt ttcctgaaat gatggatggc cgtgtcaaaa cacttcatcc
     7321 taaaatccat ggtggtcttt taggaagacg cgatctccct agccatatgg aggcaatgga
     7381 aacggaaaat atccaaccaa tcgactttgt ttgtgtcaat ttatatcctt tcaaagaaac
     7441 gattatgaaa ccagatgtcg aaatagcaga agctattgaa aacattgata tcggtggtcc
     7501 tagtatgctc cgtagtgcag caaaaaacca tgaatttgtc acctctgttg ttgatccatc
     7561 tgactatgaa gaagtacttt cagaattacg tcagtatggg catacttcac tttctttcag
     7621 acaaaaacta gcggcaaaag tctttcgtca tactgcggca tatgatgcac tgatcgctca
     7681 atacttaacg gattgggtgg aagaaaaaga accggaaaaa ttgacactta cgtatgaacg
     7741 aaaacaaacg cttcgttacg gtgaaaatag ccatcaagcc gctgcctttt atcagtccgc
     7801 tcttctggaa aaatattcga tcgcagcagc aaaacaactc catgggaaag aactttctta
     7861 taataatatc cgagatgcag atgctgcgct acggatcatg aaggaatatg acgagccaac
     7921 ggtagtcgcc ttgaaacaca tgaatccttg tggtattggg acagcatcta cgattttaga
     7981 agcgtgggaa gcagcgtatg aagcagatcc tgtttcaatc tttggtggga tcatcgtttt
     8041 gaatcgagaa gtagatctgc caacagcaga acagatgcat aagctgtttc tagaaatcat
     8101 cattgcccct tcttatacac aagaagcttt ggaattactg caaaaaaaga aaaacatccg
     8161 attactgaca gtagactttg cgcaggaaga aaatcaaaat cgagaagaaa cggtctcagt
     8221 cttaggtgga cttcttgtac aagatcaaga tctggcaata gaaaatgaag aagagtggaa
     8281 agtcgtgaca aaacgtaagc cgactccagc agaagcaaaa gcattagcgt ttgcttggag
     8341 agctgtcaaa catgtgaaat ctaatgcgat cgtactggca aacgaacgtc agacagtagg
     8401 aatcggagcc gggcagatga atcgagtcgg ttcagtaaaa attgcgattg aacaagcgca
     8461 agagaaaatg gaaggtgcag ttttagctag cgatgcttat tttccaatgg atgattcggt
     8521 tgaatatgca gccaaacatg gtataaaagc aatcattcag ccaggaggaa gcatcaaaga
     8581 ccaagattct attgatatgg cgaataaata cgatctagca atggtcttta ctgatgttcg
     8641 gcattttaga cattagagag gagtcggata aatgaacatt ttggtgatcg gtgcaggtgg
     8701 tcgtgaacat gcaataagta aaaaactttt agccagtccg aaggtggata cggtgtattg
     8761 tgcgccagga aatccgggga tgacaaaaga tggtatcaat tgtgtcgcta tcagtgagcg
     8821 agatcattca gcgttaatcc attttgttaa gaaagaggct attgattgga gtatcgttgg
     8881 tcctgaagtt cctttgttaa atgggatcgt cgatgatttt atggcggaag gattgaaaat
     8941 cttcggtccg acaaaagcag cagctctgat cgaaggttcg aaaagttttg ctaaacaatt
     9001 aatggatact tatcaaatcc cgacagctaa atccagaagt tttgaatgtt acgaaaaagc
     9061 agcagcgtat atcaaagaaa atggtgcgcc aatcgtagtg aaagcggacg gcttagcagc
     9121 tgggaaaggt gtagtcgtgg ctaccacagt gaaggaagcg ctggatgcgg cagaacaaat
     9181 gttgaaccag catcgatttg gggatagtag caaaaaaatc gtcgtagaag agtttttggc
     9241 aggagaagaa ttctcccttt tggcgtttgt ccgtaatacc aaggtgtatc cgatggtcat
     9301 ttcgcaagat cataagcggg catttgataa cgataaaggg ccaaatacag gcggaatggg
     9361 tgcttattca ccagtaccgc aaatttcaaa aaagatgatt gaaaaagctg tggaagaagt
     9421 tttaaaacca gcggctttgg gaatgaaaga actaggacgc tctttcacgg gtgttttata
     9481 tgcgggtttg attgctacga aggaaggacc aaaagtgatc gaattcaatg cgcgtttcgg
     9541 tgatcctgaa acgcaagtcg ttctttctcg gttgaagagt gactttgcgc aagtaatcga
     9601 tgatttatta gagaatcgta cagtagagtt aaagtggcag caagaaggat acaatgtagg
     9661 cgttgtggtt gcagctgccg gttaccccga agaatatgag acaggaatgg ttctaccaga
     9721 ttttctggaa gaagagcttt ctgtttacta tgctggagtt tcctcaaata acggcaaact
     9781 agttggagca ggaggacgtc tttatttagt agaagcatac ggtgagacga tcaaagaagc
     9841 acagaaaaaa atttacaccg cgttaatgga taaaacgaca gatggaacat tttatcgtac
     9901 agatattgga gaaaaagctg ccaatagcga actgatcgtg aagtaaatca gctgcttcca
     9961 tttataaaaa atacaacaaa cataatcgga agcgccgatt ttagtgaagc cttttcataa
    10021 agagtgtggt acacttttgt tgtgtatata aataaggagg agttcagatg aaaaaaattc
    10081 aattaggtac ttctgatcag aaagcagctg cagtgatctt aggctgtatg cgtatcaaca
    10141 atgcggaaga tccagtaaaa gtgattgaga cagcttatga aaacggcatt gatttttttg
    10201 accatgcaga tatttatgga aacggtgaat gtgaagaaat ctttgcagat gctctagcaa
    10261 aaacatcaat caaacgagaa gatcttttta ttcaaacaaa atgtggaatc gttccaggtg
    10321 tgatgtttga tttttctaaa aaacatatca ttgaatctgt agaaaaaagt ttgacccgtc
    10381 tgaaaatgga ttatgtggat gctttacttc ttcatcgtcc agatacattg gtagaaccag
    10441 aggaagtagc tgaagcgttt gatgaattgg aacggtcagg aaaagttcgt tattttggcg
    10501 tcagcaatca aaaaccaatg caaatcgaat tgctgaaaaa atatgttcgt caaccattag
    10561 tagctaatca gctgcaattt ggcttgaaac atactggaat gatcgatcaa gggattcatg
    10621 tcaatatgac agatgaagca agtatcgatc atgacggtag tgtacttgat tattcccgat
    10681 tgcatgatat gacgatccaa gcatggtcac cataccagta tggatttttt gaaggtgtat
    10741 ttatcgggaa cgaaaaattc cctgaattaa accaaacatt agaagaacta gcagctaaat
    10801 atcaaacgac accaactggc ttggcttctg cttggatttt acgccatcca gccaatatgc
    10861 aagtgatcgc cggtacgatg aacgtagggc gaatcaagga aatcgctcaa gcttcagaaa
    10921 ttaatatgtc tcgggaagat tggtacgaat tgtaccgtgc tgctggaaac attttacctt
    10981 aaagattgtc ataagcaggt caaaagggct ataaattaaa aagacagaat aacttgaaag
    11041 ctgttttcag cttcgagata ttctgtcttt ttttattgca aatggaaaga gtatcattgt
    11101 acggatttcg tttattagtt atttttgttt ttaaggaaaa gctgtgtccg atattctttt
    11161 ggagaaatcc cttgtttttt cttaaataaa taactgaaat agttagggtc attataacca
    11221 atttcaaaag caatatctga aatacgatag tctgattgct gcaataattt ttttgctaat
    11281 tcgattcttt gttctgtcag ataatcgatg aatgtttggt taaacgattg agaaaaaatc
    11341 gtactgaaat gagagggact taatgcaact tgttgagcga cccaagttaa ggacatatct
    11401 ggatcggtaa aattttcttt gataaaatcc aatgcatggt tcaaaattgg ctgatatttg
    11461 ttccttgaag gctgtgaggg cagagcagtt aagaaagaaa tgaacatata aactgtcgaa
    11521 cggtaaagcg agtcgtcggc gctgattgct aagcgttgac tggtggatgt gcaatgaacc
    11581 atttcttgcg ggattttctt ttctttcagg cagacatgct ggagtagctt agtcaagaca
    11641 aataaacgat ataatcgtgt tcgagttggt gtgccttgtg gtttcgtcaa gtgttcgatg
    11701 tacttagata cttcgttttt cgataatgaa gacaaatcaa gtgagaggtc aaagtaaaag
    11761 acagaatcaa ctggaagttt cgcttcttga atcaaatcct catagctgat aatttgttct
    11821 ttaagggaac tggcggtttc aagtatcatc tgcgtgatct caaaagatgc aggtaattcg
    11881 ctgattcttt ctgcgacagg accaatggcg acagaaaaat tatttttttg tcccttttct
    11941 aattcatgga ccaacgtttg ggctacctga taactggtct cgatcacatt ttcttcaaga
    12001 gaatcaacta ttaaaaattt gatgaatcga gaggaaacag cagagaagat cacttgttga
    12061 ttctccctaa ataaaaactt caaataatca ctgagatgag cgtaatcttc aaagtctgta
    12121 ctgacttggt ttgctgctaa taatacggtc atttttttcc ctgctaaaga gcgattgaaa
    12181 gattcagcaa gtttcaatgt ttcactgatc gtaagtgagc ctttgaacaa accgttcaag
    12241 aaatgatttt ttttcaattc gaaaacaaga tcagaagaag tgtgcgtttc tttttgctgc
    12301 aaatctaaag cagttactgc tttttctatt gcttgtttca attcgctact tttaatcggt
    12361 ttaagcagat aatcatctgc ctgtacctgg attgctcctt tcatataatc gaaatcgtcg
    12421 aatccactaa taaatatgag acgagtccaa ggaagtagtt tccttgcttc ttttgaaaaa
    12481 gtcaacccgt ccatgaaagg catttggata tcagtcagaa tgatgtcggg ctgtaagtcg
    12541 ataattgaag acagtgccat ctcaccatct ccagcttcgc cgacataagt gattggataa
    12601 taagaaccaa ggacagttat ttgttcccgc aagttattgc gaatgaggta ttcgtcttcg
    12661 atgataaaaa ctttatacat gtttgtttcc tccaattgtt ttgggagata gagggatcat
    12721 cagtgtgaca gttgttcctt ctcggtattt gctgtcaatc gtgataggtt tttgatcatt
    12781 tccgtaataa agaaggagac gcttgtttac gttgtaaagt ccgtagccga tactgaaatc
    12841 tgaatcgatc cctttggcta actctttacg tatttctgcc agtttttctg cagggatgcc
    12901 aatcccgttg tctataactt tgaattggat catctcatca attttgcatg cggtaatacg
    12961 aataaatcct ttgtgacgga cgtacttcgt tccatggtat acagcatttt caatcaatgg
    13021 ctgcaagatc atttttaaaa tagaaacagg aaaaagtgtt tctggtatat cgatcttata
    13081 gtcaagcata tggccgtatc ttatttttaa gatgactaga taatcattga tatggctaat
    13141 ttctttttca agaggaatcc agtcttgtcc tttgcttaaa gacgtacgaa aaaagccgga
    13201 aagtgcatgg gtcatagtca gagcttcttc aaagcagtct tgttcgatca aagaaataat
    13261 ggcatccaaa ctgttataaa taaaatgggg agtaatctga gcttgcaata cacgaacttc
    13321 actttgtgct aaatggtatt gtttcaaagc gttctcccgt aaagactggt ttagatcttc
    13381 cgccatttta ttcatgctag cggtaagtgt ttgtaattcc agtgtttccg gcagggttaa
    13441 acgaaagcca aggttccctg aagaaagttg ttctgacatc tcgatcagct gattgatcgg
    13501 ttcttggacc tttctctcta aaaagcgacg attctttttc gcatagaaaa aaatggtact
    13561 aagaactcca agttccacta ttgttaatag aatgagcgag cggacaagtg tcgtattttt
    13621 tttgcttgct aagtcgatct ccacacgaac aaaatcttgt aagatatctg acagcagttg
    13681 agtgacagaa tctacctgat tgatgatctc ttcattggta tccataaaat cttttgccaa
    13741 gatattattg atcagctcgt tttgataatc ttccagtgta tcgatcgtac gtagcgatac
    13801 atctaacgta ctaatttctt taagtgtatt tgtattattt ttgatctctc gaatatcatt
    13861 cctaagttct tctaggatat tttcctcatt gaactcatct acggttactt ggccagaaat
    13921 caagtcccac atactttcca aaactttttg atcaaccttc aaagcaattg catctgcttt
    13981 ttggatattt gcgatcgtct gttgataaat caaaagctgt tgcgaatata acagtatgct
    14041 cagtagcaaa gggataacag caaatagaag catggctttg tttgcttggg caaaaatctg
    14101 ttttatgctt aaattttttg tcatgatact tctccagtca ataatttctg gttgggatgt
    14161 tagctatatt ctgctgggaa aagacagtct cggaaataaa aatatcttta ggtaatgttt
    14221 ttcctgtttg aatatttcca aaataacgag taacggtatt ggctacatac cagccgacat
    14281 caggtttaca ctcgactaca caattgacga ttcctgagcg tagcttctcg atcatatctt
    14341 cttgtccatc aatagttaca atgatcaaat cttctccagg tttgattgat gtttctttta
    14401 tcgcagctaa agcaccgtga gtcatttcat cactatggct gtataataca tcgatttcgg
    14461 ataaatcatt tgtttctaaa tatcgtttca tctggtcttt tcctttcatc cgaataaaat
    14521 caccgacaat tcggtgaaca atagtcatcg gaggtttatg gtcgatggtt ccatcttttt
    14581 ttagggttgg tttacgattg attgattcca aaaatccttc ggagcgtagc ttggtcggtg
    14641 tggagttctc tgaaccagca agttctagga ttctgatttc ctgtttcttt gaatttgtga
    14701 agtgattgct gacaaataaa cccgcccgat ttccttctgc tttgaaactg gggccaatgt
    14761 gggtgacgaa aagctcggga tcattcgttt gaatatgtct gtcgacaagt atgacaggga
    14821 tccctgctcg ctttgcttct tttaatacag agtcccaccc ggattcttgc aaaggagtaa
    14881 aaatgatcat atccacttta taggcaatga acgtccgtat atcttggatt tgccgttcct
    14941 gattcatgta gccatttcgg tagagaactt ggtaatcttc tttttcaagc gcagtgataa
    15001 ttgattcagt atgctttttc cgccagctac tctccgttcc tgattgcgaa aaaccaacga
    15061 ctagtgggtc aggtgcttcc tttgtattag gtgtacaccc catcatttgg taagcgatta
    15121 caacaagggc taaaaaaaat aaaacacgtt tcactttaaa tcactccatc tggatttgtt
    15181 agtactatct tatcagataa aagggaaaat caaagtcaaa aggaatcaat cgaaaaaaat
    15241 ccaagaaact taaaaaaatc caagacaaaa aaagtacacc taaaaaattt aaagtaaatc
    15301 ccataaaaaa atcaatgtta acgctttctt tattagctat actcggtgta tcaattgaaa
    15361 tgggggataa aaaatgaaaa acaaatttag aaagattgtt gggactatcg ctattttgac
    15421 cgcaagcatt ctattaagtg cgtgtggcaa ttcaggatct gcagatgata gtacggggta
    15481 tgtaggaatc gctatgccga ccaaatcagc agaaagatgg atcgctgatg ggaacaacat
    15541 ggtctcggaa ttagaaaaac tgggttataa aacagatctt caatatggag aagataaagt
    15601 agaaaatcaa gtagcacaaa tagaaaatat gattacaaaa ggggtagata cacttgtgat
    15661 tgcttctatc gatggttcgg ctttaactga tgtgttagct aaagcaaaag aagcagatat
    15721 caaggtgatc gcctatgatc gtttactgat gaattcagaa aatgtcgatt attacgcgac
    15781 ttttgataat tttggagtag gtgtatcgca agcagcttat attgaagagc acttaggatt
    15841 gaaagaagga aaaggaccat tcacgattga attatttggt ggctcgcctg acgacaacaa
    15901 cgcactaatc aattacaacg gagtgatgtc tgttttacaa ccatatatgg acaacggtca
    15961 gttggtcgtg ccatctggac agacgagttt cagtcaaatc gctacgcttc gctgggatgg
    16021 ttctacggcg caagcaagga tggataatct gttaagcgca aattatactg accaaacatt
    16081 ggatgctgtt ttgtcgccat atgatccaat aagtttagga atcatttcgt cattaaaagg
    16141 cgtggggtat gggagtgaaa gtaaaccttt acctgtgatt actggacaag atgcgacagt
    16201 tgctggcgtt aaatcgatca ttgccgggga acaaacacaa acgatcttta aagacacacg
    16261 tatcttagca aaaaatacga ttgaaatgat caaagcaatc agtgatggag aagaggttcc
    16321 ggtcaatgat actgaaactt atgataatgg ggtgaaaact gtccctacgt atttagcaaa
    16381 tacagtatct gtcgataaag ataattatca agcagagctg atcgacaccg attattataa
    16441 agaatccgat ctgaaaaatt aagaggaacc aaacaaggaa ctaaagagat ctaagtcaga
    16501 gagaggataa gaattatggc ggattgtatt ttaaaaatgg cagatatcac caaaaaattt
    16561 tctggaatcc gagcgttaga ttgcgtgaat ctgatgattg aacgtgggga aatccatgca
    16621 ttgtgcggag aaaacggagc gggaaaatca acattgatga atgtcttgag cggcttgtat
    16681 ccatatggct cctatgacgg ggaaatcttt tatgatggaa agctgtgtca atttaaagat
    16741 ttgaaagata gtgaagaaaa aggcatcgtc atcattcatc aagaattagc actaagtcct
    16801 tatctttcaa taaaagagaa tatttttctc ggaaatgaac aagcagaaag aggcgtgatt
    16861 gattgggaac tgacagaaaa gaaaacatat gaactgctga ataaagtcgg tttgtctatc
    16921 gacccgaatc ttctagtatc ccagattggc gtagggcaac agcaactagt tgaaatagct
    16981 aaagcattat caaaatccgt ccgtctgttg attttggatg agcctaccgc tgcattgaat
    17041 gaagaagaaa gcgcgaattt gctgagattg atccgagagt tgaaagagca aggtgtaacc
    17101 tccatcatta tttcacataa gctgaatgaa atcgtggata tcgcagatcg aatcaccatt
    17161 ttacgggatg gacagacgat tgagacacta gaaaatgaaa tgatcagcga agagcatatt
    17221 attcgcggga tggtgggcag agatttagtt gatcgttatc ctgaaagaaa accggatatt
    17281 ggagaagttt attttaaagt agaagactgg acggtctatc accctcttga tcacgagcga
    17341 atcataaacg accacatcaa cttgcatttg aaaaaaggag agattgtagg aatagctgga
    17401 ctgatgggag cgggcagaac tgaattcgct atgagtctat ttggacattc ttatggaagc
    17461 aagatatccg gacgtatatt aaaggaaggg gcagagatat cagttaagac cgttccttca
    17521 gccattgcaa acggactcgc ttatgtctcg gaggaccgaa aatctttagg actgaatctg
    17581 ctgatggata ttcgggaaaa cgtgacgctg agcagtttgg ataaaataag tcaaaaagga
    17641 gtattagata aagaaaaaga agtaactgag gcagaaaatt tccggaaaaa gatgcgtata
    17701 aaaacaaact ccatccatca aattgtcagt agtcttagtg gtgggaatca acaaaaagtc
    17761 gttttatcca agtggctgat gacacaaccc gatattcttt ttttggatga gccaacccgt
    17821 gggatagatg ttggggcaaa atatgagatc tacacgatta tcgaagaaat ggcagcaaat
    17881 ggcaaagctg tctgtcttat ctcttctgaa cttcctgaaa tattagggat gtgtgatcga
    17941 atctatacga tgaacgaagg acgtattaca ggagaagtct cgcgagaaga tgcagatcag
    18001 gaagtcttga tgaaattaat gacgaaagaa gaggtgggtt aagttggaac aggcaaaaaa
    18061 tcttacggat aaaaaagaga aaaaactaaa tttgaaaagt agagcagctg atctttttgg
    18121 tcgctacagc atggtgatca ttttagcggg actacttatt gcgtttcaat tgatgacgga
    18181 tggtattttc tggcgtccat tgaatataac taaccttgtt ctgcaaaata gccatatatt
    18241 agtactagct gcagggatgt tattagttgt gctcttaggt catgtggatt tgtcggtagg
    18301 gtcagtgatg gcgtttgttg gtgcaattgc gggcatgatg atggtcaatc gccaaatgaa
    18361 cccgtggatg acagttatcg catgtcttgc agtaggcggg ctgatcggcg catggcaagg
    18421 tttttgggta gcttatgcag ggataccggc gtttatcgtg actttagcag gattgctgat
    18481 gtttcgtgga ctgacacaag ttgttctagg aggccaatct ctagcacctt ttccagctgt
    18541 tttccaaaaa atgtctacgg gttatttacc agacttaagc gaaggaagca gccttcactt
    18601 cttgacattg attttaggaa tgatcatcac gatcgtgtta gttgttggtc aatggcgaac
    18661 aagaaatcgt cggaaaaatc atttatttga agtggaatct ccaacagctt tcttggtaaa
    18721 ggcggtattt acagcagtcg ttgtactagg aataacttat atctttgcct cttatcaagg
    18781 gtatccgatt attttgatca ttcttggttt gatcgttgca gcatatgggt ttttgacaaa
    18841 taaaacagta gcaggtcgac agatttatgc gacgggcgga aatcgaaaag cggctgaact
    18901 atcagggatc aaaacaaagc gaatcacgtt ctgggtattc gtcaacatgg gagtcatggc
    18961 agctttagct ggattagttt tagctgctcg attgaatgcg gcaacacctc aagcaggtac
    19021 atcgatggaa ctggatgcaa tggcggcagt atattttggt ggtgcttcga cttccggcgg
    19081 cattggaaca atcatgggtg cgattgtcgg tggcttggtc atgggtgtat tgaacaatgg
    19141 catgtcgatt ttaggcgtcg gggtagactg gcagcaggca atcaaaggat tgatcttgct
    19201 tttagccgtt gtgctagact tgtataataa gaaaaaacga acaacgtagg agaacgccgg
    19261 ttattcagaa aaaagactgt gacaagacat ttgtcacagt ctttttttcg ctaaaagcga
    19321 acattcattg tttcgtttgt atagactaaa aataatatat gtttttttac cattcgtata
    19381 aattctcttt ttgctcttga tttttttgct taattgagtt aaattacata gggcgctaaa
    19441 aaaagccttg attaaattaa taatgttcgt gttttgctgg atgtgaacat caggaggata
    19501 atgtaatgaa agtatttgat tacgaagata ttcaattaat tcctaataaa tgtatcgtaa
    19561 atagccgttc tgaatgtgat acaacggtca cattaggtaa acatacattc aaaatgccag
    19621 tagtgcctgc caacatgcag acgattattg atgaaacaat cgcagaattc ttagcggaaa
    19681 atggttattt ctatatcatg catcggtttg atgaagcagc tcgaattccg tttatcaaaa
    19741 aaatgaaaaa gcgtggattg attacctcga tcagtgttgg cgtcaagaaa gaggaatatt
    19801 cctttataga gaagttggca gaagaatcgc taaatcctga ttatatcact atcgacatcg
    19861 cgcatggaca tgctaactca gttattgata tgattcagca tattaaaaaa tatttgcctg
    19921 aaacttttgt gattgccggt aatgttggta caccggaagc cgtccgtgaa ttggaaaatg
    19981 cgggcgcaga tgcaacaaaa gtcgggattg gtccaggaaa agtatgtatt acaaaaatca
    20041 agacaggctt tgggactgga ggctggcaac tggcagctct tagatggtgt gcgaaagcag
    20101 ctcgcaagcc gatcatcgct gacggtggga tcagaacaca tggcgatatt gcaaaatctg
    20161 tccgttttgg tgcgacaatg gtcatgattg gttcattgtt tgccggtcac gaggaatctc
    20221 ctggcgaaac aaaagtcgaa aatggaatcg tgtataaaga atactttggt tctgcttcag
    20281 aattccaaaa aggcgaaaaa cgaaatgttg aaggaaagaa aatctggatc caacacaaag
    20341 gatctttgaa agatacacta gtggaaatgc aacaagacct tcaatcatct atttcttatg
    20401 ctggaggaag agatctggaa gccattcgaa aagttgacta tgtcatcgtg aaaaattcga
    20461 tttttaatgg agacacaatc taaaaaataa ataaaatatt aggatgttgt gaaaaagttg
    20521 tcctgcatta agtaataaga acagccaaac aaaaatagtt ctttatatgt tttgtttggc
    20581 tggaattatt atcgcagata caagcttttg aacatcatta ttcagaataa gggactgtga
    20641 taagattttt gtcacagtcc cttatgtttt gcaaaaaaag tttttctttt ttctcgtaaa
    20701 ttcggtaaaa tcaaaagaaa aaatagacaa tagaaaagag aagcttatgt gggaaaaagt
    20761 agagaaaaaa caaggaccga tttatcgtca aattatggaa cagattatga gagaaatcga
    20821 aaacggaagg atcaatcctg gaagtcaact tgaatcagaa cgaaaactag ggcaactttt
    20881 tggtgtgaat cgttccaccg ttgtccatgc attggaagag cttagggagc ttggggtact
    20941 aacgagcaaa cgtggcagcg gatggtttgt taatcagact gaatggggga aattcgctgt
    21001 tccaagaatc gattggcggc aaatgatctc tcctcgttat gagcagacgg actggtatga
    21061 gcaaaaaata agagaagcaa agcagatcca aaaaacatcg tttcttgatt tatacgctag
    21121 cgagatgcca gatgagcttt tgccgaattt tgagtttcct tcttattctc ttgaagaaat
    21181 cttagcggaa gaaaaagaaa tggatctttt aggctatact ccgttacgta acaaaatcaa
    21241 acggtatttg gaaagtaaat ttgaattggc attttccccc aaccagcttc ttgtgactgg
    21301 tggcggtcag caagcaatct ttttgatttt acaaacgatt ctttcagctg gagaggggat
    21361 agctgtagag tcaccttctt ttttttaccg attacctctt ttcaaagctt caggaacgcg
    21421 tttatttggg ataccgatgg atgaagaagg gatcgactta gaaaaactga aagaatcgat
    21481 ccataaaaat aaattgaaag cagtgcttat caacccgaat tttcaaaacc ctacaggaaa
    21541 agtgatgagt caaaaacgac gagaaaagct agtagaattg tgtcgacagt atcggcttcc
    21601 aattattgaa gacgatgtat ttacggatct ttcatttcta aaccaagaag aagtccttcc
    21661 tatacgaacg attgatccgg ataatgtgtt atacgtcggt tcgttatcta gggttttagg
    21721 aaaaacaacg aaaatcggtt gggtgatagg acctgagact tttatctcgc aactagctca
    21781 agcgcaagaa atgatggagt tttcgatgaa tatccttaca caaatcacag tggctaatgt
    21841 atttaatgaa gcaattgatt tgaaaatgca agatgtaaga aaaaagttga aagaaaaaag
    21901 ccgactgata agcgcttggg gagattcaca atcgtttttc aagcttcatt caattttagg
    21961 tggttactat gcctggatta cttgggatgg gaaaaaacta gaccccgaac tagcagaaaa
    22021 agtagtagat atgggtcttg gaattgcacc aagtctactt tttgggcaag atataaatgg
    22081 aatcagaatc aattacagta ggctggatga aaaccaaata ccgttgtttt attcaaagat
    22141 gaaaattcta gaaaagtggt taacagaata atttcacaaa gtaaatagat taaagaatat
    22201 aacagcttgg atttaaattt caagctgttt cttttttttc ggaaaaagaa tacgatataa
    22261 agaaactaag aaaaaaccat tatttgtacg tccgttcacg atgtgtaaat aaaataaatg
    22321 tttgcaacaa aactgtataa caccaacgtt tttaaataga aacagatggt tgtaagtttg
    22381 attagaaaat agaaaaaatg cagatgaaat ataaaacgct ttccttattg tgattttatt
    22441 cactaatgct gttaatatgc ggattggcat aatttccttt catcgtgaaa atttgtgctt
    22501 ttagcttgta tttctcataa atccatgata atttattaat gaagacggtt aaatagtgat
    22561 acagttttat tcactgtttc aatcgcagaa tgaagagatt ttcttataga tgagagagga
    22621 atgtgtgaca aatggcaaac aaaaaagcac cgatcgactt ccaagcactg ctagatgaag
    22681 tcaatgcaga tttcccagta tatcaagcac ttgatcaaga tggaaacgta gttaacaaag
    22741 atttagtacc tgatttatcc gatgatgaac tcgttgaatt aatgactcat atggtatggt
    22801 ctcgtgtatt agaccaacgt tcaacagcgc tgaaccgcca aggacgccta ggcttttttg
    22861 caccaactgc tggacaagag gcaagtcagc tggctagtgc ttacgcattt gataaagaag
    22921 atgttcttct tccaggttat cgggatgtac ctcaactagt aaaacatggt ttacctttat
    22981 ctcaagcttt tttatggtct cgaggacatg ctgcaggtaa ttcttatccg gaagaattga
    23041 aagctctacc accacaaatt atcatcggtg cacaatatgt acaagcagcc ggtgttgcat
    23101 taggtttgaa gaaacgaaac aagaaaaatg tcgtcttcac ttatactggt gatggcggtt
    23161 catcacaagg tgatttttac gaagcaatca atttcgctgg cgcttaccat gcaaatgccg
    23221 tattttatat ccaaaacaac gggtatgcga tctctacacc tcgtgaaaaa caaactgcag
    23281 caaaaacact tgcacaaaaa gctgtcgcag ctgggattcc tggtattcaa gtcgatggaa
    23341 tggatccgct tgcagtatat actgtatcta aaatggcacg ggaatgggcg atcagtggaa
    23401 atggaccagt cctaatcgaa acactgactt atcgttatgg accacataca ctatctggtg
    23461 acgatccaac ccgctaccgt acaaaagaca cagatgacga ttggcagaaa aaagatccat
    23521 tgatccgctt ccgtaaatat ttgacggaaa aaggactttg gtctgaagaa aaagaagaac
    23581 aagtcatcga agcaactaaa gaagaaatca aagcagctat tgctgaagcc gacaaagtac
    23641 caaaacaaaa ggtttcagat ttcttgaaaa acatgttcga agtatctcca caatcaatca
    23701 aagaacaaat tgcaatttat gaagcgaagg agtcgaaata atcatggcac aaaaaacaat
    23761 gatccaagca atcacagatg ccttagcact tgaactagaa aatgacgaga atgtcgttgt
    23821 ttttggtgaa gatgttggga aaaacggagg agttttccgg gcaactgaag gattgcagga
    23881 aaagttcgga gaagaccgag tattcgatac tcctttagct gaatctggaa tcgcaggatt
    23941 gagtttcggt cttgcgttag aaggatttcg tccagttcct gaaatccaat tctttggttt
    24001 tattttcgaa gcaatggacg aagtagtagc tcaaatggct cgtacaagat atcgaatgag
    24061 cggcactaga aatctaccca tcacaattcg ttcgccattt ggtggcggtg ttcatacacc
    24121 agaattgcat tcggataatt tagaaggttt gatcgctcag tctcctggta ttcgagtagt
    24181 gattccttcc aacccttatg atgcaaaagg actattgatt tcttctatta gaagtaacga
    24241 tccggttgtt ttcctagaac acatgaaact atatcgctca ttccgagaag aagtgccgga
    24301 tgaagcttac gaagttcctt tggataaagc agcagttact cgtgagggaa cagatgtatc
    24361 tattatcaca tacggagcaa tggtgcgtga agcaatcaaa gcagcagaca atcttgcaaa
    24421 agaaaatata tctgttgaaa tcattgatct tcgtacagtt gcgccattag atgtagaaac
    24481 gatcattcaa tccgtggaaa aaactggtcg cgtagttgtt gtccaagaag cacaacgaca
    24541 agcgggcgtt gccgcgcaag tagtttctga gatctcagaa cgcgctatcc tttcattaga
    24601 agccccaatc ggacgagttt ctgcacctga tacagtcttc ccatttggac aagcagaaaa
    24661 tgtctggttg ccaaatgcaa aagatatcga agacaaagta aaagaaatcg cggaatttta
    24721 agaaaagaag ggacgaaaca aagtcatggc ttatcaattt aaattgccgg atatcggtga
    24781 aggaatcgca gaaggtgaaa tcgtcaaatg gttcgtcaaa ccaggagata cgatcaatga
    24841 agatgataca ttactagaag ttcaaaatga caaatcagta gaagaaatcc cgtcacctgt
    24901 tacaggaacg gttaaaaatg tgattgttcc agaaggtacc gtagcaaatg ttggggatgt
    24961 attagtagaa atcgatgcac caggacacga agacaatgaa ggggacagtg gtgtagccgc
    25021 agaatcccaa actccagcta aaccggctgc tgaacccact gttgatacag aatcagctgg
    25081 atcgtcaagc gaaggagtct tccaatttaa gttaccggat atcggtgaag gaatcgcaga
    25141 aggtgaaatc gtcaaatggt tcgtcaaacc aggagatacg atcaatgaag atgatacatt
    25201 actagaagtt caaaatgaca aatcagtaga agaaattccg tcacctgtca cagggacagt
    25261 taaaaacgtg attgttccag aaggcactgt agcaaatgtt ggggatgtat tagtagaaat
    25321 cgatgctcct ggacacaata gtgcatcgtc aactagtgcg ccatcagctg aagcaccaaa
    25381 agaaaaagtg gaaacatcag gttcagcaag tgtcgttgaa gctgccgatc caaataaacg
    25441 tgtattggct atgccatctg ttcgtcaatt tgcccgtgaa aaagatgtag atatctcaca
    25501 agtaactgca actggaaaag gtggcagagt aacaaaagag gacatcgaaa acttcttggc
    25561 aggtggtcca agcagtgcac cagcgaaatc agaagcgcca gaagcggcag caccgaaaga
    25621 agcagctcct gcagcagaaa gcaaaccagc tgcaccagca aaaccattca agtccaactt
    25681 aggagacttg gaagaacgtg tagcaatgac gccaacacgt aaagctatcg ctaaagcaat
    25741 ggtcaacagt aagcatacag ctccacacgt gacattgcat gatgaagtag aagtgtctaa
    25801 actatgggat aatcggaaac gatttaaaga agttgctgct gcaaatggta cgaaacttac
    25861 tttcttgcca tatgtcgtga aagctttaac agcgactgtt aagaaatacc cagtattgaa
    25921 tgcatctatc gacgatgcaa accaagaaat cgtttataaa cattactaca atatcggtat
    25981 tgcaactgat acggatcacg gactatatgt accaaatgtg aaagatgcag accgcaaagg
    26041 aatgtttgct attgcagacg aaatcaatga gaaagcaaaa ttagctcatg acggcaaact
    26101 atctgcagaa gatatgcgca atggaacaat tacgatcagc aatatcggat ctgtaggtgg
    26161 cggttggttt acaccagtca tcaactatcc agaagtagct attttaggtg taggaacgat
    26221 tgcacaacaa ccaatcgtaa atgctgaagg cgaaatcgtt gtcggtcgtg tgatgaaact
    26281 gtcattaagt ttcgatcacc gtatcgtaga tggagcaact gcgcaacaag caatgaataa
    26341 tatcaaacgt cttttagctg atccagagct attaatgatg gaaggatgat aaacaaatgg
    26401 tagttggaga ttttgctgta gaattagata cagttgtaat tggtgcggga cctggtggct
    26461 atgtagctgc catccgcgct gctgaaatgg gacaaaaagt agcaatcatt gaaagagagt
    26521 atattggcgg tgtttgtttg aatgtcggct gtattccttc aaaagcactg atagctgcag
    26581 gacaccatta tcaagagtca ctagattctg aaatgtttgg agtaactagt gaaaacgtca
    26641 aactagattt tgcaaaaaca caagaatgga aagaaaacaa agttgtccat acgttgactt
    26701 ctggtgttgg tttcctatta aaaaaacata aggttgaaac aatcgaagga gaagcgttct
    26761 tcgtagatga tcacacacta cgcgtcattc atccagattc agctcaaact tattcgttca
    26821 ataacgcaat catcgcaaca ggttcccgtc caatcgagat tccaggattt aaattcggag
    26881 gacgtgtgtt agattctact ggcggattag cattgaaaga agtacctaag aaatttgtga
    26941 tcatcggtgg cggtgtgatt ggtgccgaat tgggcggagc gtacgcaaac ttaggagcag
    27001 aagtaacgat tcttgaaggc tcaccacaaa ttttaccaac ttacgaaaaa gatctagtaa
    27061 aattagtaga agatgatttc aagaaaaaag gcgtaacagt cgtaacgaat gcaatggcga
    27121 aagaagcagt cgataacggt gacagcgtga cagtgaaata tgcagtggac gggaaagaag
    27181 aatccgttac tgctgattat gtgatggtca ctgtaggtcg tcgtccgaat acagatgata
    27241 tgggcttaga acaagcgggt gtcgaagtag gagaacgagg attgatcacg gtggataaac
    27301 aaggacgtac gaacgtacct aacatctatg ccatcggtga tatcgttcca ggggcggcat
    27361 tagcacacaa agccagttat gaagcaaaaa tcgctgctga agcaatctct ggcaaaaaag
    27421 tagctgtaga ttataaagca atgccggcag tagccttcac tgatcctgaa ttggcttctg
    27481 taggtatgac gatcaaagaa gcaaaagatg ctggtatcga agcaaaagca tataaattcc
    27541 cattttcagg gaacggtcgg gcattatcgc ttggtaaaac agaaggattc atccgtctag
    27601 ttacgacgat cgaagataac gtgcttattg gcgcgcaaat cggtggagtc ggcgcaagtg
    27661 atatggtctc tgaattagct ttagcaatcg aatcaggaat gaatgctgaa gatatcgcct
    27721 tgacgattca tcctcatcca tctcttggcg aaatcgtcat ggatgcttcg gaattagcat
    27781 taggtttacc aatccatatt tgatttgaat aatacaggcg aaacgcaact gaaaaatcag
    27841 ttgcgttttg ttttttagaa ttatcaataa tattattatg taaacttaaa aatatgacaa
    27901 cttcttggtt tattaccact tcctatgcta cactgaagag gaatatgaaa taggagagtt
    27961 gaagatgcaa gaatatcagt atccacttga tttagattgg acaacagacg aaatggtcat
    28021 tgtaatgaag atgtgggaag atctggaacg tgcgaatgaa aaagggataa acaatgaagc
    28081 ttttttgaag acttatcagc agtttaaaac tgttgtcaaa tccattggtg aggaaaaacg
    28141 attgggacgg gaatttgaaa aagcttccgg ttattcttta tatcgtacag tgcagcaagc
    28201 aaaaaaaaat ccaaacaaaa ctttgagaat gaacgggtga gataaatgga acagatgatt
    28261 gatcatatca agtcctggct tgaagaagct ggtgagaaaa tcaaatcttc tcaacaaaca
    28321 ttaacaatct cgcaaaaatc aaatcgaacg gatctagtaa caaatgtcga tcgaaacatc
    28381 cagaattttc taatcgaaaa aatccattcc tttgatccag aggcgaaaat attggcagaa
    28441 gaagaagggt gtaatcaatt atcggatctc gatggaagag tctttatcat cgatcctatt
    28501 gatggcacct tgaattttgt gattgaaggg gaaaattttt gtattatgct cgctgtttat
    28561 gaatccggcg ttggaaagct tggtttcatt tatgacgtga tgagaaatga acttttttgg
    28621 ggtggaaaag gaataggcgt gtataaaaat gatgagccat tgcctagacc agcaaataaa
    28681 tccttatcgg aaggcttact tggtgtgaat agttatcttt tcggtcataa taaatatgga
    28741 atccgagaaa ttgcggaaag cagcatgggg acacgtatgt acggatgcgc tggtcttgaa
    28801 ctagtggcaa tcttgaaagg aaaccacatt gcttatattt caaaccttag cccttgggat
    28861 tatgcagcgg gaaatgtctt attggaagag tttggtatgg taacaagcgg tctttttggt
    28921 actcctttga catttgaaaa aagggaatac tatttagcag ggacttcttg tgcatatgca
    28981 gaaatcgcag accttttgaa aaaagaaggc tgaaaaaaag gtagaaatca ttcacaaaaa
    29041 tgataaaaac gagcaaaaga ttaattttct cgaaaaaatg aaaacatttt actatatttc
    29101 atgaaaaaag tctgctataa ttagcagtcg agtaaaattt gccctgtcat aaagaaccaa
    29161 taaaaaagga gttagttaca ttgaattata gaaacgatat ccgtaacgtc gcaattattg
    29221 cccacgttga ccacggaaaa acaacattag ttgatgaact attaaaacaa tcagatacat
    29281 tagacgcaca tacgcaattg caagaacgtg cgatggactc aaatgcgcta gaaaaagaac
    29341 gtggtatcac gattttagcc aaaaacacag ctgtagacta taaaggtatt cgcgtaaaca
    29401 tcatggatac tccaggacac gcggacttcg gtggcgaagt agaacggatc atgaaaatgg
    29461 ttgacggcgt tgtgttggtc gtagatgcgt atgaaggaac aatgcctcaa acacgttttg
    29521 tattgaaaaa agcactggaa caacatatca caccaatcgt tgtcgtaaac aagattgaca
    29581 aaccttctgc tcgtcctgag catgtcgtag atgaagttct tgaactattc atcgaattag
    29641 gagcagatga tgatcagtta gactttcctg tgatttatgc atctgcacta aatggtacgt
    29701 caagcttgtc agatgatcca gcagatcaag aaccaaccat ggcacctatt tttgatacaa
    29761 ttattgaaaa aatccctgct cctgtcgata acagcgacga accattacaa ttccaagttt
    29821 cattacttga ctacaacgat tatgtaggtc gtatcggaat cggacgtgta tttagaggaa
    29881 caatcaaagt aggagaccaa gtagcattaa ttaaattaga cggaacagtt aagaaattcc
    29941 gtgtaacgaa attatttggt ttctttggac taaaacgtct agagattcaa gaagcaaaag
    30001 ctggagactt gattgctgtc tcaggtatgg aagatatctt tgtcggtgaa acagttactc
    30061 ctgtggatca tcaagatgcg ttacctatcc ttcacattga cgaaccaaca ttgcaaatga
    30121 ctttcttagt taataattca ccatttgccg gtcgagaagg taaattcgta accgctcgta
    30181 agattgaaga acgtttgatg gcagaacttc aaacagatgt atccttacga gttgaaccaa
    30241 caaactctcc agatgcatgg acagtttctg gtcgtggaga attgcatttg tctatcttga
    30301 ttgaaaacat gcgccgtgag ggatacgaat tgcaagtatc tcgtccagaa gttattgaaa
    30361 aagaaatcga cggtgtaaaa tgcgaaccgt ttgaacgcgt gcaaatcgat acaccagaag
    30421 aatatatggg tagtgttatc gagtccttga gcttacgtaa aggtgaaatg caagatatgg
    30481 tccatacagg aaacggacaa atccgtttga cattcttaac acctgcccgc ggtttgattg
    30541 gttattcaac tgaattcctt tcaatgacac gtggttacgg aatcatgaac catacttttg
    30601 accaatatct gccaatgctt cctggtcaaa ttggcggacg tcaccaaggg gcgcttgttt
    30661 cgatcgatac aggaaaagca acgacttatt ctatcatgag tatcgaagaa cgcggtacag
    30721 tcttcgtaga accaggtaca gaagtatatg aaggaatgat tatcggggaa aacagccgtg
    30781 ataatgattt gactgtcaac atcacgaaag caaaacaaat gacaaacgta cggtctgcta
    30841 ctaaagacca aacatctgtc atcaaaaaac caaaacaatt gacgttggaa gaatctttgg
    30901 aattcttgaa tgatgatgaa tattgcgaag taacaccaga aagcattcgc ttgagaaaac
    30961 aaattttaga gaaaaatgct cgtgaaaaag catctaaaaa gaaaaaataa tctgtaaaag
    31021 gatgaagaga gaaaccagct tgttataaaa aatagctggt ttcttttttt gtccaactct
    31081 tttctgtgga gaagtcatct ttgtcaatgg acataaattc agaaaatacg ccgaaagacg
    31141 tttaaaagac ttttaaaaaa cttatttcta gagagttttt gtttcataat aagataatgt
    31201 ttcacgaaag gagatcaact tgtccttcga agaaaatcga agtaaaatgg caagagaaag
    31261 gagcgggaag atgaacgact tattaaaaca atactttggt tatgatgagt ttagacctgg
    31321 tcaaaaagaa attatccaga aagtcattga tcaggaaaat gtattaggaa tcatgcctac
    31381 cggaagtggg aaatcaatct gttaccagct tccagcttta ctattagatg ggcttactgt
    31441 ggttgtgtcc cctttaatct ctttgatgaa ggatcaggtg gacgcggcga atcaattagg
    31501 tatacccgcc acttttatca atagctcgtt agatggttac gaaacagcta gacgcttcca
    31561 agaaatcgat agacagcagt accgtttact ttatatcgct cctgaacgtt ttatcatgcc
    31621 ggattttatc caagcaatga agcgatggaa cgtccgtatg atcgctattg acgaggcaca
    31681 ttgtatctct cagtgggggc atgatttccg cccaagttat ttacagatgg cgaaccagct
    31741 agatcaactg ccaaatcgtc ctgtaattgt tgctttgaca gctacagcga ctgtgcaagt
    31801 agctgctgac ataaaaagac ttttaaaaat tccagaaaac aaccatattc agactggttt
    31861 tgaacgagaa aatctacgat ttcaagtaat taaagatcaa aaaaaagaac aatatctaat
    31921 cgaatattta aagatcaaca aaaatcaatc tggtatcatt tatgcagcaa cgagaaaaga
    31981 agtagaccgt ctctatcatt tgcttaaaaa attcgatttt tccgtgggta gatatcatgg
    32041 tggtctaaat gagaatgagc gaacagaaat gcaagaagcc tttttatacg atcgcttgca
    32101 gctgattgta gcgacaaatg catttggtat gggtataaat aaaagcaatg tccgttttgt
    32161 tatccattat caaattcctg gctctctaga agcatactat caggaagctg gtcgggcggg
    32221 aagagatggt ctttccagtg aagccatcct gcttttctct cctcaagata tccaagtcca
    32281 aaagtttttt atccagcagt cccaaagaga agaagggcaa aagcaaaaag aatatgaaaa
    32341 attaaaagcg atgacagaat acgtgtatat tgagtcctgt cttcagcaat acatcctaaa
    32401 ctattttggc gagacaagca gtccgtgcaa ccgttgtggt aactgtttag atgacagaga
    32461 aatagtagaa gtaacgacag aagcacagat ggttctttct tgcttaaaac gtatgggtga
    32521 gaactatggt aaacaaatgc tgatgaaagt cttagcagga tcgaaagaac aaaagctgca
    32581 agctttaggt tttggacatt tatctaccta tggactgatg aaaaaccaat cgcaaaaaga
    32641 aacgatgcag ttaattgaat atttgatatc gaatggctat cttctgacca tcaatggtga
    32701 gtatcctgtt ttgaaagtaa cagaaagagg gatacaagta ttgaaaggac aagaatctgt
    32761 ttatcgaaag gaacccaaga aagtccagca gctatcggat gaagaaaccg atacactatt
    32821 tgaagtgctg cgggaactgc gaacggatct agcatcagaa gcaggcgttc ccccgtatgt
    32881 cgtattttca gattccacat tgaaagaaat gagccgaata cgtccatcaa gcagattgga
    32941 gatgcttcag ataaaaggag ttggccaaag caagcttgat aaatatggcg aagccttttt
    33001 atctcggata aaaaatgcgg actctaacgt tctggataag tagaaagaac gaatacgtaa
    33061 aaatgaataa aaacaactgg ttaaatctgt tttttttgct ccttatgaaa atcaaatatg
    33121 ttataattaa ggaattagaa tgggaggtaa gcacagatgg attctttgtc gaaaaaactt
    33181 gcgatccaag tattaaatga tgaagcagaa cggatcaaac gattaatccg aaatcaaaac
    33241 aacagtctct gtatctcaca atgcaaagcc ttcgaagaag tggtagatac acagatgtac
    33301 ggcttttccc ggcaagtcgg ttttgctgtc cgtataggag taatcggaaa gaacgagggc
    33361 cagctgatgt taagtgagct agaacgagaa ttgaatcagt tatatagtga ggtttaccaa
    33421 gaaatttatg ataaaaaaga agttggcaag gaggaataga cttgcctaag aaagtgaaaa
    33481 aaaggcatct attagactat agcatcttga ttccctattt aattttatgt gtattgggat
    33541 tgatcatggt atatagttct acgtcgtatc tattattaga aaacggacaa aatccgtccg
    33601 catcagtcat taaccagagt attttttggg tgcttagctt gattgctatc gcactacttt
    33661 ataaaatgaa aacagatgtt ttgaaaaacc agcgtttgat catggcagcc atcgcagttt
    33721 tgacgatttt gctgctgatt gttgtgtttt ttggtaagga gatcaatggg gcgaaaggtt
    33781 ggctgcaaat tgcaggattc tcgatccaac ctgccgaata cctaaaaatc atttctatct
    33841 ggtacctgtc tctgacttta tctaaaagac aaaacagtgt acaaaaagat tttcttggga
    33901 ctgtgaaacg tccacttgca atggttatcg gacttactgc attagtcgct attcttcccg
    33961 attttggtaa tgcggcagtt atctttttga ttattttggt tctgctttta gccagcggag
    34021 tcaattatgt gtatactttg attgtaggag taggaggctt ttgtctaagt acttttacga
    34081 tctggctgat caatatcaca aacggaaaga tcttacccgg acgtttacag tatatctata
    34141 atcgatttgc tatttaccaa aatcctttct cagacgaatt aaataaaggg catcaactag
    34201 tcaacggcta ctatgccatg ttcaacggag gactatttgg caggggatta ggtaacagta
    34261 tccagaaaaa aggattttta caagaagcac aaaccgattt tatttatgca atcgtagtgg
    34321 aagaactggg agtgattatg gggattttga tattagcgtt gctattcttt atgattgtgc
    34381 gcattgtcct tgtaggtatc cgttcaaaag atccgttcaa ttcgcttttg tgcattggga
    34441 tcggcgcaat gtttttgatc caagtatttg tcaatcttgg aggaattact ggcgttattc
    34501 ctttgacagg gatcacgttc ccatttttaa gtcaaggagg ctccagtttg ctgatgcttt
    34561 cgatttgtgt cggctttgtc ctcaacatca gtgcggatga aaaacgcaag agcttaggtt
    34621 tataataaaa aaagatgtag gagtggttcg atgaaaaaag ttttagttgc taacaggggt
    34681 gaaatcgctg tacgtgtatt tcgtgcttgt acagaattag gaattaaaac tgttggaatc
    34741 tatgcagaag aagatgaata ttccgtacac cgtttcaaag cggatgaagc atatttagta
    34801 ggacaaggaa aaaaaccgat tgatgcgtat ctagatattg aagggatcat ttctatcgcg
    34861 aaagaatgcg gagctgacgc tattcatccg ggttacggtc ttttgtcgga aaacttgaat
    34921 tttgcgcaac ggtgcgaaga agaaggcatc atttttgttg gacccaaact gcatcattta
    34981 gatatttttg gagataaaat caaagcaaaa gctgcagcta tcgaagcggg gattgcttcg
    35041 attccaggca cagatggacc aatcgcttct atagacgatg ccttggagtt tgcgaaacaa
    35101 tacggttatc cgattatgat caaggcagct ctaggcggcg gcggacgcgg catgcgtgta
    35161 gctcacgatg aaaagagtgc aagagagggc tacgaacgtg cgaaaagcga agcaaaagca
    35221 gcctttggta gtgatgaagt gtatgttgaa aaatacatcg ctaatcctaa gcatatcgaa
    35281 gtacagattc tcggggatac gcatggaaac gtcatccatc tttttgaaag agattgttct
    35341 gttcagcgcc gtcaccaaaa agtagtagaa gtagcgcctt gtgtctctat gaatgaacag
    35401 caacgtcaaa aaatctgcca ggcagctgtc caattgatga aatatgtcgg gtatgtcaac
    35461 gcaggtacag tagaatttct tgttgaagga gatgattttt actttatcga agtcaaccct
    35521 cgcgtccaag tggaacatac gatcacagaa atgattacag atattgatat cgtaacaacg
    35581 caactgttga ttgctcaagg tttggatctg cataaagaaa tcggattgcc tcagcaagaa
    35641 ggaattaaat taaatggttc agccattcaa tgccgaatca ctacagaaga cccgttgaat
    35701 aactttttac cagacacagg gaaaatcgat acgtatcgtt cacctggtgg ttttggcgta
    35761 cgtctggatg tcggaaatgc ttacgcgggc tatgtagtga caccgtattt tgattcattg
    35821 ctagtaaaag tatgtacgca tggtgctacc tttgaaacag ccattcagaa aatggaacgt
    35881 tgcttgaaag aatttagaat ccgtggagtg aaaacaaaca ttccatttat gctcaatgtc
    35941 atcacacatc ctgaattcca atcaggaaat gcaaaaacaa ccttcattga tagtacagca
    36001 acattatttg aatttcctcg actacgagac cgcggaaaca aaacgatgaa atatatcggt
    36061 gaaattactg tgaatggttt tccaggtatc gaaagtggcg aaaaaccatt ttatgaagaa
    36121 ccacgtatgc ctaaagatct aatcacaaga actgattacg taacggctaa gaatgtatta
    36181 gatgcgaagg gtgccgatgc tttggtcgaa tggatcaaag gacaggaaaa tttgttgttg
    36241 acggatacga catttagaga cgcccatcaa agtctgctag caactcgtgt tcggacaaag
    36301 gatttcaagc agattgcacg tttgactgga gaaggtcttc ctgaactctt ttcaagtgag
    36361 atgtgggggg gagcaacatt tgatgtggct taccgctttt tgaatgaaga tccttggcaa
    36421 cggttgagaa aaattcgttc tttaatgcct aatacgttgc tgcaaatgct tttcagaggt
    36481 tcaaatgccg ttgggtattc taattatcct gacaatgtat tggtggaatt tgtcaaagaa
    36541 gcagcagcgc aaggaatcga tgtatttcga atctttgata gtttgaactg gactccacaa
    36601 atggaaaaaa gtatccaagc tgtacgggat actggtaaaa tcgcagaggc agctatttgc
    36661 tatactggcg atatcaacga tccaagtcgg gcaaaataca atgttcagta ttataaagac
    36721 atggcaaagg aactagaaca actgggtgcg catatcattg caatcaaaga tatggctggg
    36781 ctgcttaaac cgcaggcagc ttatcgtttg attagtgaat tgaaagaaac gacagatctg
    36841 ccaatccatc tgcatactca tgataccagc gggaatggaa tcatcactta ttctgctgct
    36901 tcaaaagcag gagtagacat cgttgacgtg gcaatgagtg caatgagtgg gaatactagt
    36961 cagcctagca tgagcagctt gtattatgca ctggtcaatg gtccacggct gccagaaatc
    37021 acgattgaaa atgcacagaa attaaaccat tattgggaag atgtgcgcat gtattacaaa
    37081 ccgtttgaaa acggcttgaa tgctcctgaa acagaagtat atatgcatga aatgccagga
    37141 ggacagtatt cgaatctaca gcaacaggca aaagctgtcg gattaggtca tcgctgggat
    37201 gaaatcaaac aaatgtatca tactgtcaac ttgatgtttg gcgatatcgt taaagtaact
    37261 ccatcatcta aagtagttgg agatatggca ctatttatgg tccaaaatga tcttacagaa
    37321 gaagatattt atgaaaaagg cgaaacacta agtttccctg aatctgttgt gactttcttc
    37381 caaggagaac tagggcaacc cgttggcgga tttcctaaaa aattgcaaaa gattattctt
    37441 aaaggcagac ctgcattgaa tgaacgtcca ggtcttttgg ctgaatcagt cgacttcaac
    37501 gaagtaaaaa aagaattggc agaaaaaatt ggctatgaac caaaacaaga cgaagtatta
    37561 agctatttga tgtatccgca agtctttttg gactatcaaa aagcatataa tcaatttggc
    37621 gatgttacat tactggacac accaacattt ttccaaggta tacgtttggg tgaaacaatc
    37681 aatgtacaaa tcgaacgcgg aaaaacgctt atcattcgtt tagatgaaat cggagaaccc
    37741 gatatcgaag ggaatcgcgt actgttcttc aatttgaatg gacaaagacg tgaaatcact
    37801 atcaaagata attcgatcat cagtacggtc caaacccgat tgaaagctga accgacaaac
    37861 cgagaacaaa ttggagcaac gatgtctggt tctgttttgg atgtattagt gaaaaaaggc
    37921 gataacgtta aaaaaggtga tacgttgatg attactgaag cgatgaaaat ggaaacggcc
    37981 atcgaagcac gttttgacgg agaagtcgca catgtatatg tgtcttcagg agacactatt
    38041 tcgtctggtg accttttgat tgaagtgact gagaaataat aagcgaaagg tggggaaaca
    38101 tgaaaagact gggtttgttt tggctgattc tgctattagt tattgtggca ttctacatgg
    38161 aaccagtctt gtttccgcca aaacttgaag tgaaggaaaa ctctgatcaa caaatatcaa
    38221 accaaatgac aacgtggaaa taccaggagc ttaaagcaac tggtttttct acttatatcg
    38281 gaaaaccgat cgatcagctg gaaaaggatt ttggccaagc ctatgatcga ttaactagcg
    38341 gatttggttt tgaaacaaga tattatgaaa ataaagctag tcatctcaca tttgaagcga
    38401 acatccaaga cagcagagtc acaactgtta aagtactgca aacagggcct gctgatatcg
    38461 cgccgtttta tttagggatg acgatgcaag atcttacgaa aatcactact atctatacta
    38521 atttcacttt tgagtaccat gatacagaag ttggaatcga actaatggaa gaggatatga
    38581 attatcgtcc gctaattgct tttgataacg acacttttgc tattttattt tttgatcaaa
    38641 caacaaacgg cctattttca gttgtgtatt tagataaaga tagtcttttg aaactattgc
    38701 catatcaagt gtttggtgaa ggccttccac attatcaagc agataaatcg gctgattggg
    38761 aatcaatcaa tcgcgttaaa gaacgaaaaa gtacgacttt attaaatatg cttagaagag
    38821 aagacgaact tcccatatac aatcaaacac ttgcctttca aaaccacagt cagctgctac
    38881 tacatgattt tttgacaagg cctgaggaaa tattgtcaga ggaacgtatg gctgattggc
    38941 aaaaatcgct aggcagtgct caaacgacag tgaaattctc actgacgaat gatgaattgg
    39001 agaagttaat cagtaaacaa aaattagaaa atactagtgg gctatttatt catccggcga
    39061 ttgacgctac tttttcgttt ttatattggt acagcgatcc ttacaaccat gaccgtttca
    39121 tggacccggg aacggattct gtaggcattg ctttttctaa agaaaatatg ttagtcttat
    39181 tgcaagaaga aaacggagaa agtagtgata gcagtgatcg ttaacgaaga gctgtttcaa
    39241 ctggaggatc aatgcctcca agtcgctgaa atgataaaaa agagtaaaac gatggcttct
    39301 tatcttgaac ataaacagtc aatggaagaa tctgcaaaag tttgccagtt gaaagaagcg
    39361 ttcttgtcta agaaagaagc atttgaaaat gtagcggcat atggtgagta tgcacctgat
    39421 tatcgtgaaa aaaaacgagc tgttcgaaaa gcaaaaagac agcttgatat ggatgagcat
    39481 gttgcagctt tccgatatag tgaaacacag ctgcaaagtc tgttggatca aattgtccaa
    39541 gtacttgccg aagaagtttc taatgaaatc aaagtagatg caggtagccc gttttttgaa
    39601 acgaaaaaac actttggatg cggaggtaat tgtcatgcaa ttagacaata gtgaatttga
    39661 aataacaaaa cgacgcgggc tgatcgtatg ggtctacagc ttaaaacaat taaagaatct
    39721 gaaaaagttc ggcttgttgc attatgtctc aagaagaatg aaatatgtcg tgctttactt
    39781 aaatgaagag acattggaag aaacagaaga acgaatccgt aaattacatt ttgttcgaaa
    39841 cgtagaacgt tcttaccgac cggatatcga aatgaatttt gccgaaaaaa taggaaacaa
    39901 aacagtatta aaagacgaag gtttcgaaat tgaagagcta aatacagaaa tccgattagc
    39961 tgaacatctt taaaaatctc ttatgagcag tttttaaata aaaaataact tggataacga
    40021 tcgatttttg atcattgtcc aagtattttt ttggtatact agtaccagta ccatttctaa
    40081 ggagtgagta gatgcggata atatcaggaa attatggcgg gcgccgttta aaaagtttag
    40141 caggggcgaa caccagacca actacagata aagtaaaaga atccatattc aatatgattg
    40201 gaccatattt tgatggagag acagtattgg atctgtttgc aggaagcggt ggtttagcta
    40261 tcgaagcagt atctagaggc tgcagtcatg cggtatgcgt agacaaaaat taccaagcgc
    40321 taaaaatcat caaagaaaac atcgaaatta cgaaagaacc agagaaattt accacaataa
    40381 aacttgatgc ggacaaagca atcgaacggc tagcagcgga gaaacaacaa ttcgactact
    40441 tgtttcttga tccgccatat gcaaaacaaa aaataatcga tcaaatccat aaaatggaag
    40501 aactgggact tttttctaaa accgcagtta tcgtatgcga aacggataaa acagtcgaat
    40561 tgccagaaac tgtggctgga ttccatcaaa taagaaaaca gatttatggc ataacggccg
    40621 taactatcta tagaaaagag ggaaatcaat gaaacgtgca ttattcccag gaagttttga
    40681 tccttttaca aaaggtcact tagatacggt ggaacgtgcg gctaaattat tcgatgaggt
    40741 agtaattggt gtttttatca atacttcaaa gaaaagtttg tttcccccag aagaaagaat
    40801 gacattgatc acaaaagctg tttctcatct tcctaatgta aaagtcatgc atcaagaaaa
    40861 tcaattgacg gtagaaacag caaaagagat tggtgcagat gcgttgatca gaggcatacg
    40921 aagtatcaaa gactttgaat atgaacgaga gattgctcag atgaatcatc atctatatga
    40981 tgaattggaa actgtcttct tattagcaaa accagaatat agtcacgtga gttccagtat
    41041 tttaaaagaa gtactgcact ttggcggaga tgtgagcagc tacttaccgc cagtgatcaa
    41101 cgaagcttta gctggaaagc gtgaatcaaa tgaaagctaa acaatggttg aaaacagtac
    41161 tgattatcat atttggaatg ttgatcattg gatttgtagt agttcccttg ccgtattatg
    41221 tagaatcacc gggagccacg atcgatctga aagaactaat tacggtcaat gataaggaag
    41281 ataaaggttc tgggtctttc tctcttacct ctgttggtat caggcaagct acaggtttct
    41341 tagctttaaa agctaaaatg accccttttg aagaattgat cagtgaagaa gaattgactg
    41401 gtggggcgac aaatgaagaa tataatcaaa tgcaacagta ttatatggat tcttcccaaa
    41461 atgcagcaat cgaacaagcg ttaaaacttg cagacaagcc ttataaaatg gaattcaaag
    41521 gcgtatatgt catgagtgta gagaaaacat cgaatttctt tggcaagctt tcagttggag
    41581 atacagtaac aaaagtagac gggaaaagct ttcaaagtac agaagcattt atggattatg
    41641 taaaaagcca aaaagttgga caaacaatcg aaatcagtta tttgcataat ggtgaagaaa
    41701 aaacagcttc tggtgatctg atcgagttac caacagataa aaaggcagga ataggtatca
    41761 cgcttactga ccataccgag atcgaatcag atacagatgt tcgtattaat tctggctcta
    41821 tcggtgggcc atcagcggga ttgatgttca ctttagaaat ctatgaacaa gtcactggta
    41881 aaaacttgcg acatggtaag caaattgcag gaactggaac gatcaacagt gaaggagaag
    41941 ttggccgaat cggaggaatc gataaaaaag tggcaagtgc ggataaagct ggcgtagaga
    42001 ttttctttgc accagatgat acgatttcag acgatgttaa gaaagaatat cctgaaataa
    42061 aaaccaacta cgaagaagca aaagctgccg ctaaaaaatt gaattcctca atgaaaatcg
    42121 taccagtaaa aacagtacaa gaagcgcttg actatctaga gaaaatgaaa tgaaactaaa
    42181 taattaaaga ggggttgtga cacgagttct gtcacaaccc ctctttttcg tcaaaaatct
    42241 tcacgataaa aataaaaaca aaagaaaaaa ttgacgtaat tagatgttga agggacgatt
    42301 ttgccagagc tcactgtttc ttcaatttta aatgaaagga gtaatgaatg atttttactg
    42361 gtcattcttt gaaaaaagaa caatgataga aaaaatcagc aagaaaatac tagcttcacc
    42421 caaattgatc atttggatgt ctatgatagt tcttatcttt tgtatcgcaa gtggtttttt
    42481 attatcaaat ttgctatttt atagagaaac agaagagact gctgcaaaag aaatactttc
    42541 ttcgtttagt tccagtggag aagatccatc agctgaagga aacgatgtag aagaggatgc
    42601 tcgtccaaag gtcatgtaca ccgatatcaa aggaagtgtg aaggagcctg gtatctattc
    42661 cttttcttca gaagaacggg tctatgatgt actaaagcgt gcaggtggat tattagaaga
    42721 agcggatagc gatcgaatca acttttctgc gaagatagaa gatcagcaag tcctatatat
    42781 ccctgctgta ggtgaagagc tgccagaaca tcttgatcag ttggcatcct cggaaggaaa
    42841 acagcctaca gcagattccg agcctcccaa aataaatatc aatacagcca ctccatcaga
    42901 acttcagcag attcctggga tcggatcagt caaagcgcaa gaaatcattc gatttagaga
    42961 agaaaacgga tccttccaga aagtggaaga cctgcaggag atttctggta tcggtgaaaa
    43021 gacagttgaa aaactaaaaa acttcgttac aattaaatag aatgattcaa aatcgactgg
    43081 cacgagcaat aaatggacgc taatggatac taagaataat ccaattctaa gagcttggca
    43141 ggacgaggag gtagtatttt tggataggaa atagcttgat tgttcgcaaa gaaacattaa
    43201 aaaaaccaat gctgaatgtc tatctccaaa ataaaatatc tgggattcat attatgaata
    43261 cggctgtcag cggcaataac agtcaagctc tacgagaacg ctttgccaaa gatgtactta
    43321 gctatacagc agataaagta tttattctca ttgggacgaa tgatctagca gagcataaac
    43381 aactttctaa agaaacgtac caaaaaatct gcagtggatg atagttcagc tgaaaaaaga
    43441 ctatagttct gatcatgctt tctttatcac gccaccagca gtcgatgaga caaaacaaaa
    43501 atatcggaat aacaagttga tcaaagaata tatctctgtt ctcacagaag tagcaaaacg
    43561 ccatgattgt gaagtatcga tttttatacg gaatttcttg aacaaatgga ttcgaagaat
    43621 ttttataatg ggctaatgga tgacggactt catttcggca gtgaagggta ggatcttttg
    43681 tcagacagga tgattcgtta tatttaaaga atattttgtt taagcaatga gtacttttta
    43741 atcaacttga aaaaacggag gaatataaaa catgttagaa aaatcttcaa aagcatgcga
    43801 tacgaataaa gaaaaagtta ctgataagtc acataatgaa cgcattcctt gggaccagta
    43861 ctttatggct caggcagtac tgttatcttt acgtagtacg tgtacacgtt tagaagtagg
    43921 agcgacactc gttcgagaaa aaaggatcat tgctggcggc tataatggag cagtcagtgg
    43981 agatgtccac tgcatagatg aagggtgtta cgtcgtggat ggacactgct tgcgaacgat
    44041 ccatgcagag atgaatgcac tcctacagtg tgcaaaactt ggtatcccga cggaaggttc
    44101 agaaatctat gtcacacatt ttccatgttt ggcctgtaca aaggctttgt tgcaagctgg
    44161 tgtgaaaggg atcaattatc tccatgacta tcgaaatgat ccttatgccc aagcgttgat
    44221 cgaacaatta ggtgtatctg tacaccaagt tgctttagac tcacagcatt tttcaaaact
    44281 tcaaaatgaa ttaacaggaa aacatgtcta gcgttttaga aaggcatgtt cgaaatcggt
    44341 tgatttttcc ggcaattacg tcgatgatta tctgtttgtt gtattacact gacaattggg
    44401 gttatcgttt gcttcttgcc ggaatttttt tatggttttc gctaaaagaa ggacgtttta
    44461 tcgcgtactt gtgtattttg tgtagcataa cggtatccat ttcttatagg ctacaagagt
    44521 attcattttt agaggaaaaa acaacatctg cgcaaattac tgtatttaca gatacgattc
    44581 gtgcaaatgg taattttttg acaatggaag gaaggttatc agagaataaa caaaagctcc
    44641 agctttctta tcaagtaaac acagaagcag aattgaaaac atggcttaac ttccgaggaa
    44701 agggaataga attgactgct gaaggcgagt tcatctctcc agcaaaaaag cggaatcttt
    44761 ataattttga ccaagaagct tacttcagga accatagaat ttctggacgg ttccaaattc
    44821 aaaagattga acagatcacc atgcaaaatc actggcgaaa ctggcttcag agaaaaagag
    44881 gatcttttat ctcctttgtc caatctgaat ttccgcaaaa aacaagtgta tatattaatt
    44941 ctttattatg gggctataaa gatgcaggat tcagagaacc agaagaaatt tttagggaga
    45001 gtggtttgct gcatctgttc agtctatcgg gtctacacat ccaattttat ttagggtggc
    45061 tgtattattt atttcgacgt atcggatggc cactacacgt cagtattatt ccgttgagtc
    45121 tactaactat ctgttatgtt tctattgcag gaagttctat ttctgttttg cgtgctagtt
    45181 tgctgttttt attgcgattg ctagtcaaat tgctgaataa gcattattcc accatggact
    45241 tatttgcagt tgtcttttgg cttcttttac tatttgatcc tcgatttctt tttcatgcgg
    45301 gtggacagct tagttttttc atgagttttt tgtttatcct gatttctttt gatggagatt
    45361 ggttgaaaaa aacggcctct cacgtcctgt ttactatgat cacgatgccg ttgattgtat
    45421 ggcattttta tgagtggcct attttaggta gtctttttac attactgatt gcaccggtat
    45481 tcaagtttct ttttcttcct ggtgtgttat ttctcacact cttcaatcgc tggctgccgg
    45541 aaactttact tttgctgata gaagagggat taatcttatt tgagcgaata atcgattttt
    45601 cttcagggtc gtctctcatt ataggacgtt tgccccttgt gctatctggg atgtatattt
    45661 tcggattact gatttgtatg gataggttga aagaacaacc tgtgaaacac ttgttttatg
    45721 ttggtctttt ttcttttatt tttttgactt tgccattttt acgcttttct tcaacgatcg
    45781 cttttatcga tgtcggtcaa ggagacagta tctttttgca aagtcctttt cgaaaagaaa
    45841 cgattttgat cgatactgga ggtcagttac actttgaaag agaaaagtgg gcgcaaggta
    45901 tgatacgtcc tcctgctgaa agaaatatcg ttccatatct aaaaggtcag gggataagtg
    45961 taatcgataa actcatcttg acacacgacg atacggatca tgtgggagaa ttacccacgt
    46021 tatctcagca ttttactatc aaaaaaattt atatcggttg gggcgctgga cgaaatgagc
    46081 cattacaaag gcatttatct gaagctcaaa aaaatggtac tgagatcatt gaaatcaaac
    46141 atggggacag gatcagcggt tattatgatc tttatgtatt gactcctttt gaaaagggag
    46201 agggaagaaa tgaagattcg attggtctat ggatggaata taacaatagg cgctttttat
    46261 ttttaggaga tttgaatcaa gcaatggaaa aacaattgct tttgatttat cctaatctga
    46321 aagccgatgt agtcaaatta ggtcatcacg gtagccgtac gtcaagtaac acagattttc
    46381 tttcccatat tgaagcaaaa catgggatta tatcctgcgg cgtaaataat cgttatggtc
    46441 acccgcacag cgaagtattg gagacactag aagaaaatca gataaaaaca tggcgaacgg
    46501 atcaacaagg aatgatcacc tatcgctggc atccagtttt tcatcctcat gggctagtgg
    46561 aaacaatgat agactagtaa ttctcatctg ctcatggtag gataattttt gaaagtggtg
    46621 agtaatttga gtatacaagc agagttacaa aaaatacgta aagaaaaact tgcatcttgt
    46681 tacttgattt taggaacaga aaagtttctc caagatcaag ttcggacaga aattgtcaaa
    46741 aaaatccaat tagagggcga agatgactta aattttttaa gttttgacat ggaaaatgca
    46801 agtttagatg aggtagtagc tgaagcagag acgttgccgt ttttcggaga tcaacggtta
    46861 gtattcgtag agaatcctta ttttttaaca ggtgaaaaag taaacaatgg aatcgaacaa
    46921 aatactgatt tattgacggc ttatttgaaa gaaccattag aatcgactgt attagtattc
    46981 tttgcgccat atgaaaagtt ggatgaacgg aaaaaagtaa cgaaacaatt gaaaaaaaca
    47041 gcaattatca tcaatgtcca acagttgaat gaaaaagaag tacgtcaata tctgatgaat
    47101 accttagaga attcctcaat caaaatggaa cgaagtgcaa ttgatttatt cttgaggttg
    47161 accgacttag atttatccaa aatgatgaga gagttacaga aattggtcct ttatggacaa
    47221 aatcaacagc aaatcacggt aagagaagta gaagaattag ttcctaaaac gttagaacat
    47281 aatatttttg acatgaccca atatatttta aaagggaaga cggaacaagc tttgcgttta
    47341 tacgaagatt tagttatgca aggggaagaa acaatcaaaa ttaatgcgat tttgctttct
    47401 caattacgtt tatttttaca gacaaaattt cttgtaaaga ttggttatca gcaggcaaat
    47461 attgctgaaa cattgaaaat ccatccatat cgtgtaaaac ttgccatgca ggaagtaaaa
    47521 aaattcgatg aacatttgct ggtacagttg tttgatcaat tagttgagat ggattatcaa
    47581 atcaaaacgg gacaaatcga aaaagaatta agttttcaac tattcgtttt gcgcacaggg
    47641 cagttgttaa gaaatcgata agaagaaaga cgagggcaaa gcatgcggga aggtcgtttg
    47701 tttttgtcct ttttttgaca aataaaaaaa tcggccgaag ccgattcttt tatttagcga
    47761 tttttttgct taaacgagat ttgtcgcggt tagctttgtt tttgtggatt agaccttttg
    47821 attcagccat atcaatagct ttaacagctt ctttatataa cgcatctacg ttgtcagcac
    47881 ctgaagctac agcatcttca aattttttga ttgcagtacg catagcgctt gtttgagatg
    47941 aatttttcac attagcgttt tcgcttgtac gaacacgttt gattgcagat tcaatgtttg
    48001 gcattagttt cacctccgat aacttaagtt cttacgcgtg ttaccgcata attcaacatt
    48061 cattattata cctaaaggca gcgtgctttg caatatgaac atgacaagtt tttttcctta
    48121 acagtctttt attaggattt tttttcagta aaaacggata gtacaagctg aaaaaaatca
    48181 aaaagacgct attgtgggag gcaatagcgt caaaccaaac tagattttta tttatctatt
    48241 taaacaaagt ttgaatggga atgtttggta ctaagttatt atataacaaa gagaaccgtt
    48301 tgcaaagttt tttttgaaaa gggatacgat tccctgttgt tctttgtttt tctattaaga
    48361 aactgcagtt attttataac cagtatcctt tgatcagtaa aggctattct ggtgtaccga
    48421 ttattttaat gtattcatcg atatttttta tcaacatttt ttttatgacc aactggttcc
    48481 gacggttcca tttgccactt acttccagca catatttatt taggggcaaa cgtaagatat
    48541 attttgccaa atagcgatct ttaagcaggc cattgatcac acggtcctca agctgcaagt
    48601 tgatcccagt cagcatcatt ggctgttctt gtaaaacgta cgtatcaatt aaaagacctt
    48661 caaaagtttc cacaagcgtc ttcctttctt ttcaaagtaa cctgaaaaca ggcactaatt
    48721 cttccatttt gatattcgtt gaagtggatg cgatcattct ttcttttgtc cactgtaatc
    48781 tagtataaca aaaaagagca gaagagccaa aaagaaaaaa gagcatcgaa ttctatagga
    48841 agtctcattg gcacggccta aattattaca aaacattgat taaaaaaagc ccctgcatcg
    48901 caggagcatc ccctatatcg ttttaatagg tcaatcaaaa agcagtatca aacacatttt
    48961 gtatcaaatc aggaaataat agataagcta agcccagaat caaaagtacc gctagaagaa
    49021 accagccgat tgttcctatg aaatacttta atttttcgaa cattgtatca cctcgttgtc
    49081 agtatatcac ggatcagtaa attgggggtg ttaagaatct cctaaaatag acaaaatttg
    49141 ttctattttc ctaaagcaaa tacttgtaag atacagtaag tgataccaga agcaatgacc
    49201 ggtccggcgg cgattcctct taagaagaca acacctaaaa tcgtgccgaa aacaagtgca
    49261 acagtgattt gtggatcaga agcaatcaat cctacaccct tggaagataa gacagccact
    49321 agcccaccgc aaaaaacagc tacccagcca ataggggatt taaaggcttc taatagatca
    49381 cgtagaccga tttctcctgt tgcgataggg attagaatag ccgctgtgat gatcgtcacc
    49441 ccaatattga ttcctttgcc ttgaagccaa gggaaaaagg aacgggaggc tggtatgagc
    49501 ttcataatca aaacaaaagc tgccgctatc atcagacttt gatttttagc taaaattgct
    49561 acaagtaaaa taattcctaa aaaaagccaa ctttccatga aatatattcc tcactttctt
    49621 cgaaacaatc ataacatgtt tttagaaagt tacaaaagta ggaattcatc cttcaagacc
    49681 tattcataag ctgttttttt cataaaataa acaaaatcat aaaaaaacac tttgcttatt
    49741 tgacagaaga cagccgcgct ttgtatagtg gacaagactt acattttttt gaagaatcag
    49801 aaagatcagg taaaggtctt agtaaacgca agccattcag aataaccaat attgtgcttc
    49861 cttcgtgtcc tacaactcca agaggtagat ttaaaaattg caggaagtta gaagcaatca
    49921 acaagagaat aacaagaatc gaaaaaataa tattttgttt gatgatccgc cgcattttta
    49981 aagatagccg atgactgtag acaaatttat ttaagtcgct tttcattaag acgatgtctg
    50041 cgatatccat agcggcatct gtcccttgcc ccatagcgac acctaaagaa gcggctgcta
    50101 aagcaggcgc atcattgact ccatcaccaa ccatcgcatt tacttggtaa agttcattct
    50161 cttttttgac tagttgtgtt ttttcttccg gagtacagct tgcatagtaa tgttcaatcc
    50221 ctacttgttc agcaatggcc tttgctgtgc cagaatggtc gccggttatc atacttgtat
    50281 ggatgttttg ggatttaaag taggaaatta cttgttgggt atttgctttt ggaatatcta
    50341 gcagacctaa aacagcaatt aactgctgat ctttagaaag ataaatcacg gttttgcctt
    50401 gattttccaa acgttcgatc atttccgcaa tctctttgct gattatcatc gtctcaggat
    50461 catatgcgtg cttgccaact ttccattgcg cacctaaata ggtagtttgt aatccaaaac
    50521 cagtaatttc ttctattggt aagtattgga tttcttcagg tattgtgcaa tcgaaatatt
    50581 gcaaaatcgc atgagcaagt ggatgcgtcg ttttttgttc catagcaaca agaagttgtt
    50641 gtgccaaatc cttttcatca aagaaaaagc tgtcagttac aactggtttt cctttagtga
    50701 gcgttcctgt tttatcaaag gcaatcgctt ttaattcaga aagcttttct aaatggatac
    50761 cgcctttgat taaaatccca ttttttgcag aatttgaaag agcagctaga gaagctggtg
    50821 tagcagatgc gacaagcgca caaggagaag ccacaactaa taataccatt cctcgataaa
    50881 agctttcatc ccaactccaa cctaggaaaa agtgggggag aaggatcatt agaggaacag
    50941 caagcaaaat caattttaca taattatttt cgattcgttc gataaatgaa gctgtttgag
    51001 ttggcgtatt ctgcgcttct tctactaatt ggataatctt agcaaagaca gtttcggaac
    51061 tcgtttttgt tactcgaatc gtgatgggat ttccttgatt cagcgttcct gcatacatct
    51121 catcgcctaa atatttttct acaggcacag attcaccgct taatgcagac tcatcgatcg
    51181 ttgcctgtgt gctttctaaa aagccatcta tcggaacagc tgctccttta gcgacaaata
    51241 ccaagttatc cacctttagt tgagaaacat ctacttcttg catttgtcca ttttctttta
    51301 ataattgggc tttttgtggt tgaagattca tcaaagcagt aatttctttt tgacttttat
    51361 tagttgtgta ttcctctaat gcaccgctta aacaaaagat gaatgtcaac attgctcctt
    51421 caaagtaatt accgatcaaa caggcaccaa tagcagctaa agccatcaat aaatccacat
    51481 tcagtgtccg atcattccac aattctttca agccttccga tgtttgttta tatccaccga
    51541 tgataatggc actgatataa aaaagtgggg tgatggtaag agagaatcgg tcgaagataa
    51601 aaccgaagat cataaataaa aggcagccga ttgttgctgc aaaagctttt ttctgattag
    51661 tcatcgttat tagacctcct tgtcaaaaca aggatagcat aataaaaatg taattgataa
    51721 taataacagc taaatgagaa cgataatggt tatcatttgg tgattaagct tgatgttgtt
    51781 tttattattt ataatgatta ttattaaaga aaataaaaaa ccagaacatt tctcaaataa
    51841 gagaagtgtt ctggtttttt ctacattgca ataaaccaat aaccaatcaa aatgatacca
    51901 aggataatac gataccagcc aaaagcggta aagtcgtttt tcttcaaata gcttagtaag
    51961 aatttgatag ctaagacgga aaccacaaaa gatacgaatg ttcctacaag aagaacaaca
    52021 gtttcttcca ttttgaatga attcccttca gctaaatatt tgacgatctt caagccgctt
    52081 gcgccaaaca tgacaggaat ccctaagaaa aatgaaaact ctgtagcgac aaaacgcgaa
    52141 gcaccaatca agatagatcc taggatcgtt gcgccagaac gggatgttcc gggaacaagt
    52201 gccaagactt ggaataaccc aacaagaaga gctgcttggt acgtaaagtc attcagagaa
    52261 gtccaacgag gtgttttgtt tttgttgcgt ttttcgacaa taataaaggc aataccgtaa
    52321 acgatcaaaa caatggcaac tggtaagaac ttgtggaaat ttgcttctaa ccaatcatcc
    52381 aaaggcaaac caatgatgat ggctggtatg caagcaacga tcactttaaa ccaaagtgtc
    52441 cacgtatctt ttttttctac cgttgattta cttggagaaa atgggtttaa cttgtgaaaa
    52501 taaagaacga cgacagccaa gatcgcccct aattggataa cgacattgaa catcgacata
    52561 aattgtgaac tggcatccag tttgatgaat tcatctgcta ggatcaaatg accagtacta
    52621 ctgatgggca gccattccgt tattccttca ataataccga gaatcactgc tttgataata
    52681 tttgcaaaaa acataaattc cttccttccc aattaagata catcttatag tataccgtta
    52741 ttttttgaga aacccaatct gttttttaat attaagaaaa acttgagaaa taaagcagat
    52801 aagtacacga agagaaaaaa gaagacaaag ccctcgttca gactttgcct tttatgattc
    52861 tctggtaaaa attaaaatat ccccattata gaaacgagtc gcgactatgt gttttcccgt
    52921 tatcgatata agacgtatca ttgatgccgt ggatagaaaa tcgaccgttt gcataagtga
    52981 cctttgttac gctgccattt aaaagcccag cacgaacagg ctgtttagga tcgatcagat
    53041 taagcaaaaa ggcgatcgtc aaaccatgag atacgactaa aacatttcca ccaccatttt
    53101 tttcgcagtg atgggcgata tcttcaaatc ctgtccatac acggtctcgc aatgtttcat
    53161 aagtttccgc ccacccagcg gtatctaagt tgtatacagc attagcaatt tgttcaaaag
    53221 aaacttctgt ggtaaacatt tcatcatatg ttttaaaatt caatacacga gggatcacac
    53281 cccacatttc catgtcatat ccaccttcaa gagacccgaa acaccattct ctaatccttt
    53341 tttcaataaa ataaggaatt tcttttccct tagaatgttc tcctagaata atacgggcag
    53401 tttcgattgc acgaccactg tcactagaat aagcaaatga aaagtcgaca ttttttaacc
    53461 cgcgacctaa ataatgaatg ccttcttgtc cttgctttgt gaggggtgta tcacaccagc
    53521 cttgtactcg ttcaattgta ttgaacatcg tttttccgtg tcgtacgata taaagtgtag
    53581 tttctgtcat tttattctcc tcctaagtcg ttttaacgat tgaaaaaagg atttgtcctt
    53641 ttttcctgtt caatcgtcgt tgcatcgcca tgtcctggat agactgcaaa ttcatcaggt
    53701 aatacaaaca gctgggtccg aatactatga agtaattgtt ctaaattccc tgtatacaga
    53761 tcggttctac cgatacttcc tctaaacagg gcgtcgccag aaacgacaaa atcatcaaaa
    53821 ataaagctga cgcttcctat agaatgccct ggggtaggga cgacgcgaaa agacatgttt
    53881 cctaaagtat aagttttcat ttcaaattca tattctgcag gcgagacaat aacatcagca
    53941 atatcatcat gtctaccgag tccagaaaga ttcaaaatcg gatcacctag ccatttttgc
    54001 tctagtgggc tgacataaac tgggatttga taatacgcgc gtaaaggttc cactgcaccg
    54061 atatgatcgt agtgagtatg ggtaagcaaa atcgctaccg gctgttggtt tgtttttttg
    54121 atttcttgtt tgattcgttc cgcatcttct ccagggtcaa tgatcaaaag tgcttcttcg
    54181 ttgtagacca aataacagtt ttcttcaatc attcctgttt ttaatctttc gatatgcatg
    54241 tgcattcctc cttgatagcc aaaacttctt tcatccatta gtataacgca ttcattgcta
    54301 ggaaacaaaa acaggtactt atttctcttt acttatctga attatcagac tatatgaaaa
    54361 gtatttggat attctaaaga gaaggaaagt aagttttcat agcattttaa tatgtccttg
    54421 ttacattaga ttcatccaaa aatccaaatt ctatatccta tatccctgcc taatcagcag
    54481 ggtgtttttt tatgtttgga aagagagctc tttttgctaa actaggtttt ttcaggtaat
    54541 cttagagaga aagaggtggt ggaaatgatc aaacagtcaa aaatatctgt gcggcactta
    54601 gtagaattta ttttgcgacg tggaagtatc gacaatcgta aaaaaagtaa ccatactgca
    54661 ttagaaggag cgagaatcca ccgcaagctc caaaaagatg cgggagaaaa ctatgaaaaa
    54721 gaagtctttt taaaaacaac agttcaatta gatgaatatt acctgatcgt tgaaggccgt
    54781 gcagacggta ttttcaaaag agacggtatt tattatatag atgaaatcaa aacctccgaa
    54841 ccacgatttg aagatttaga accagaacaa gtggaacttt tctttcatca agcaagggtg
    54901 tatgcctata tctatagtca agaacatgat ttacaagaaa taaatctgca attgacttac
    54961 ttccagacgg cagaagaagt gatcacgaga aaggttgaac atcagacaag agagcagtta
    55021 gaaacttttt ttaaaaagct gactgaagat tataaagaat ggcttgtttt tcaagaaaac
    55081 tggcgcactg tacgcaatgc ctctttgatg gctttgaaat tcccccatga tgagtatagg
    55141 aaaggtcagc gtgaactagc ggtagctgct tataaaacga tccgtacgaa acagaaattg
    55201 tttgtagaag cacctaccgg aacaggaaaa acaatctcta cgttattccc cgctttgaaa
    55261 gctgtagggg aagaagaggg agaaaagatc ttttatctaa cggctaaaac aataactaga
    55321 caagtagctg aagatgctat gacagcatta aaagatacag gagctgaagt aaaaagtgtc
    55381 actttgacgg cgaaagacaa gatctgcttt ttagatgaga cgacctgtaa tccagatcag
    55441 tgcccatatg ccaatggtta ttacaatcgg attaatgaag gtttatggga tcttttgaat
    55501 catgagaatc agatcactcg tgaagtcatc gaaacttatg caagaaagca tacactttgt
    55561 cctttcgaac tatcattaga tgtcagtatc tggtgtgacg taatcattgg ggattacaat
    55621 tatttatttg atccaactgt ttatttaaga cgtttttttg aagatgaaaa aaacgaggac
    55681 tatctgtttt tgattgacga agcacataat ttggtgaatc gttcccgaga gatgtattcg
    55741 gcagaactat cttatgaaaa aacgaaacga agtaaggaag ctataccaaa ggaattcaaa
    55801 aaacttcatc gccgtttcaa caaactatta aaagaatttg attcgatccg agaaatagca
    55861 aaagaagatc attgggatta tcatcatcaa aaagcaccgg ctgaatcttt agtaaaggca
    55921 ggttatcaat taagcgaaaa aattaaagaa tggctggcag aatttcctga gcacccatca
    55981 caagaacaat tgcttcctta ttattttgat ttgcttcatt ttttgaaggt cagtgaattt
    56041 tatgatgatc actacgaaac aacagtcgaa aaaacgtatc gcgatctgat agtaaaagag
    56101 ttttgtattg atccaagctt gtttcttgag cagtcgttgg ataaaggaaa aagcagtatt
    56161 cttttttctg ctagtttttc accactctct tattatcagg aaacactggg gggacaaaaa
    56221 agtctggctt acaaactgcc gagtcctttc ccagaaggaa accagcaagt tttcatcgca
    56281 aattacctag agacgactta tcgaaaaagg gaacacagtc tggcaaaatt agtagagacg
    56341 attcatgctt ttgctgatgg aaaggtcggg aattatttcg ttttctttcc ttcttatcaa
    56401 tatttagatc ttgcggtgga agcattcaga cagcaatatc ccgacagcaa tgtgctaatc
    56461 caagaaacgg acatgaatga ggaagaacgc gaacgatttt taacaaaatt tcaaacaaat
    56521 cccgctgaaa cattgattgg attttgcgta cttggcggga ttttttcaga aggaatcgat
    56581 ttgagagggg accgattgat cggcagtatg atcgtaggtg taggtttgcc gcagatgaat
    56641 catgagcagg aactgatcaa atcatattat gatgagaagg aacaccttgg atttgcttac
    56701 gcttatcagc ttcctggaat gaacaaagtc ttgcaagctg gtggtcgagt gattcgtggc
    56761 atggcggatc aaggaatcgt tgtattggca gatcaacgat tttctagttt ttcttatcga
    56821 cgtttattcc cacaacattg gcaaacagca agagaagtac gaagtgttgg ggaattggaa
    56881 gaaggaatac agcgtttttg gttatcaaaa gaagcagtaa atcaaaaaaa cgaggaatga
    56941 cagacgtcag cctcgttttt tgattaacga tagttagtga attgcaactc gattggtaag
    57001 tcaagtttac gtaataaagc aatcacagct tgtagatcat cacggttctt acctgtcact
    57061 ctaatctgat cttcttggat ttgtgctttt actttcagtt tttcattttt gatagctgta
    57121 gtgattttct tcgcgttttc acgatcgatt ccgttgacca aatcggcagt ttgtcgagct
    57181 ttgccaccta gagcgtgttc tgattcggaa aaatggatat ttttgattgg cacatttctt
    57241 ttgatcagct taccaaataa tacatctttg atctgttcga ttttgaaatc atcatcgctc
    57301 aatacaacta gattgctgtt ctctaatttg atttctactg tagaatcctt gaagtcaaag
    57361 cgattggaca attcttttgt tgtttgctga atcgcatttt ttacttcttc tgtattgact
    57421 tctgatacaa tgtcgaagct agcatctttt gccataggaa ccctcctgat atacatataa
    57481 atatttacca tacatctatc ttatcatagg attagggcag attcttacct gtttgctaaa
    57541 attcttctca tttgagttcg aagtatttta ttttaataga taaactgtta ttcttacatt
    57601 tggaatacca aagtagggga gaggtgcttg tgagtaaaat tatgactgcg tatcataaaa
    57661 atgaaagatt aaagaaaata gtggttattt tgataaccgg attgaccgct gcggtcgctt
    57721 taaattattt tttgattcca gccaatgtct tttctgcagg gatgaatggg atcgctcaaa
    57781 tcatttcctc gcttttatat gaatggtttc atatcaagat agacacagga acatttattt
    57841 ttcttttgaa tatccctgtg tttgtattag ggtttctcaa agtaggaaaa cgagcaacga
    57901 tccttagttt tgtgaatgtt gtatgtgtat cggtgatgac gatgctgctt ccagtcggac
    57961 agatcactga taatattttg atgaatgccc tagtaggcgg tgttttatta ggtcttggcg
    58021 taggattatc tttaaaaatg ggctttacaa ctggtgggat ggatatcatc tctcttgtac
    58081 tttcacaaac aactggaaaa acagtcggaa actacatgct gattctaaat ggtatcattg
    58141 tagtcatcgc aggcttcttc tttaactggg aaagtgcctt gtatacgatt atttccattt
    58201 atgcaatgac tcaagtaatt gatgccatcc acacgagtca tcaaaaagta acagcaatga
    58261 tcgttaccgt caagccagaa gaggtaacag aagaactagc aaaacggatg gtacgtggaa
    58321 tgacattact gccgtctgtc ggtggctatt caaaaaaaca agggaacatg atcatgatgg
    58381 tcatcacccg ctacgaaatg tatgatcttg atcagatcgt tcatgaaatc gacgacgatg
    58441 catttatcaa tattttacca actcattcag tatttggccg gtttgcaaat gaaaacgaac
    58501 aaaaaatgta tcgaacaact ggcgtattgc cagaaataaa aaatcgttca caaaaaaaat
    58561 gaggaatcag gtttatttta gaataaattt aatctattac atctaataag aacattttta
    58621 taaatttaaa cgagaatctt acttctaaat tctcatttac aagcagctaa aaaaagagta
    58681 tgataaataa aaatcgtgtt aaggagtgtc gtacgatgag cgaagcaaac caagaacggt
    58741 ctggacaaga aatagaacta atcaaagaac gtattcttgc tgccttggaa atggtgattg
    58801 atcctgaatt aggaatcgac atcgtcaatt taggcttgat ttatgatata gaattcaatc
    58861 ctgaaaatgg agaaacagtc attaagatga ctctaacaac tatgggctgt ccattagcag
    58921 atattttaac agaatctatc catgacgcat taaaagaagt gccagaagta acgaaagcag
    58981 aagtcaaact agtctggtat ccagcatgga cgacagataa gatgagccgt tatgcacgga
    59041 ttgcgttggg gatacgatag aaatcttggc tgactagcta tgagaagcaa ataggaaaaa
    59101 caaaaaagcc ttttatcctt tctagaagaa aagagataaa aggtttcttt gtttataaaa
    59161 ttttgtctgc tatattataa gttaattaaa taaatgtgtt ctattgataa aggacattca
    59221 aatccctttc cttttggaat ccttcttttt tacatcagtt tgattttcgt atagtattct
    59281 ttctcatctt cacgataaat cgcagcccca tttttttgtt gtgctttaag actgccaaga
    59341 ttttttttat ttacagacaa aagtgcctca tcaatgccga ttttttttgc ctcttgtagt
    59401 actaatgcta atccttgtgt ggcatatcct tttttacgat aattagggga gatgccgtaa
    59461 ccaatatggc cagcgccgtt tcttaatgtt tcgttcagtt tatggcgcaa attaaaaatt
    59521 cctacatact catgttcatc actttctaaa ataaagacgg ttgcgggaac gtaactctca
    59581 ggaagatcga ttcctaaaga cgttttttcg cattttttta cataatctaa aaattcagca
    59641 aatgtatagc cataagcatt gttgataaat ccactctcat ttggtccaaa gctagtttgt
    59701 agctgataag ctttctttaa atctgcttcc catagtttga tcagtttcat cgtattcacc
    59761 tctttaagta accttattca tttgttttct actatagcac tattgttgta aatagccatg
    59821 cctattttta tttattgtgt catatcgtat taagaatcat cgaacaatac aaatatgaat
    59881 tacaataaaa aaattatttg aagttcgttc attcgttact tatgatctct agaagaaaga
    59941 aaacgtgaat tacttcagcg tctagaattc cagaaggata gtaatcaaca gaagaaataa
    60001 tcaatatcca aatgaaagaa gatagctgaa tcgagaaaaa gaggaaaaaa gaaatgaaga
    60061 aaaaaataaa atagttacta atcccagagt cgttcgttta acattgaaat cgatcatatg
    60121 gataaaacag attttttcac caaccctcaa tagatgatgg agttgctcgg atgaattatt
    60181 tgtgtagatg gcataaggga gggcgtacag aaatgaaaaa gaacaaaaat atgttcttga
    60241 caaaaaacac atgtaatcct aaactaaaag atgtgatgcg gctatggcgg aattggcaga
    60301 cgcgctagct tcaggcgcta gtgagggtga cctcgtggaa gttcgagtct tcttagccgc
    60361 ataatttgtt tttttataaa gagaaggtac acctggagcg atgagaaaga aaataacata
    60421 ttatctaaaa gaatatagga aaaattttaa acaacggaac cagattcaaa taaatcaaaa
    60481 aaaatttgtg tgaaggtggg acagtttaaa acctaataga ttgattcaaa tagtagattt
    60541 atagattctg atacaagaaa tactggcaaa cgatagttaa ggaataaaga ctaaacttaa
    60601 actcaaaaag aggtcatgcg aaagaataaa tcgcatgacc tcttactata ttttgaatgt
    60661 gtttaaacgt tcaaataatg aatcttagta ccaaccgttt gcttcccaga atgctttcgc
    60721 cgcttcccaa gaaccataac gtgaagtaac atattgttct gctactcttt cttggtttgc
    60781 agcagagtag tcaccattta agtaagaaga atctaattgg taacggccga tgtaacgtcc
    60841 gttcgttgct gtgtaagaac cgcttgattc tttttgtgcg atccattctt ttgcagagct
    60901 tgtacttgct ggagtagcag cagtttctgt tacagcagga gcaggttgtg tttcagcaac
    60961 aggcgctgct tgtgtttccg tttgaaccgt ttcttgtaca acaggttgtt cagcttgaac
    61021 aggttcagct tggactggtg cttcttgaac ggctgcttga ggttcagcag ctgcttgtgc
    61081 agagccttct gttgggattg ttaattgttg tcctgagtag atcaggttaa tatcagaaat
    61141 tgaatttgat tctgcaatgg cattgatcaa ggagttatca ccaacgtatt tttgagaaat
    61201 cgttgataga gtatcacctg attgaacggt ataagcttcg tccgcatgag cagtcgttcc
    61261 catgaagaat gcagcgccgg cagccaaagt tgttccaaaa agtaaagttt taagtgatgt
    61321 catgaaaaat ctcctttatt cgttaaaaag ttttatttgt tgtgttcaac gaggaatact
    61381 ttaacacgtg aaaatatttc ataggtaaaa gaagcatgac aatttatgac aaatccacgt
    61441 tttttgtaac agaatgatgt tcgctcgatg tgaaaaatga tgaggaattt cacataatgc
    61501 agaaataatg ggattagaac aggtgttctg atgagaaaag aaatgtataa ataacttgaa
    61561 agaaattgct tttgttcatc attttattta catattgcgt atattttttg tatttttgct
    61621 tgagatgtta caataaaaga aaaaggaggc ggttccaatg agtaaaagat accagaacat
    61681 catggtagca gttgacggat caagacaatc gatcaaagcg ttcgctgaag cccttgactt
    61741 agcaaaggac aatgaagccc acttatttat cgtgtcaatc atcaacaaag ttgagctgac
    61801 ccatagtgcc tatgcctttt caaaaatcta tgcagatgaa aaacaaagaa cagaagtagc
    61861 catgctgaaa aaaatcaatg atgccaaaga gtacggcatt gctgagatcc acgcaatagt
    61921 tgaaactgga gatccaagaa atttgattgc caatgtcatc cctggacaag aaaatattga
    61981 tttgatcgtt atgggagcga caggaaaagg tgctatacaa caagcattag ttggatcgac
    62041 cgcttcttat gtcgtatcac atgccccttg cagtgtgttg gtcgtgaagt aatgagataa
    62101 gggaggaata gaaaatggtt cgtcctcaag aagtccgtgc gccaaaagaa aaaatagaaa
    62161 ttttagcaat tattgaagac ggcacacaaa caaagaaggg atactcgatc gctttagtaa
    62221 agtggaaagg aaaaaaaggt gttgctctga gatgggatgg agataaccaa caagataaag
    62281 gattccctgt cacagctaac ggttatcatc cagcgtggtt tgttctgcct gataaattta
    62341 tggaattata cagctatgat tatgcaagag ccgcagcatc aatcaaagca ttagataaat
    62401 taacagaaga gcagaatgaa atggaggaaa aataaaaaag acgaaccaat cagcctttag
    62461 aaaactgatt agttcgagga gtaaaaatga aaaaaggtta tttgggatga aactagaata
    62521 atgcgtataa cttaaataaa acttaaagca ataaaatggc aagagggtga ggcaaatgga
    62581 agcaggaaaa gtattacagg aagtaaaagc actatgtgca gataaaaaat atgaagaagc
    62641 gaaaagcctg atcgaaaaga acaaagaaaa tttggaagaa aagtacccga tcgcacaaga
    62701 atttatagaa ttaaaacaag gaacgatcct tcaccgattc aaacattttt ttggtataac
    62761 agaatagaaa aaacgattaa agatcggcag aaatttgctg catctttaat cgttttcttt
    62821 ttaaacatgc gagggattca attctgattc ttatataaaa aatagatcca aataataaga
    62881 aaaattactg aaagcgtcaa ttcgatgatg tgtgttatta atacagacgt tgtttcccaa
    62941 tgcctaaaag aaaaaatttg ataagtaaaa tatacagcag gtaaagaggc cagtaaaact
    63001 gaaaacttct tttttgtcca ttttatgtaa tagaaaagaa tgcttatcat aataaaaaaa
    63061 ggaatataaa aaacaaattg accgatcgcg atcatactat tgcctccgat aaataaagtc
    63121 catgatacaa aataacactt tgtgcgaaat ggcgcaagca gttttgtgga tttatccggt
    63181 aaaatctgta tcgattttga aattagagga gaaacttgct ataatcaaag tgaaagaagg
    63241 agtgggattt tcatggatca tgtagtgtta gaaatcgatt tgcatttgat caataatatt
    63301 cgggtgattt actatgttgt aagtaacagt gtcgaacaac gtgtactgac gaataaaata
    63361 aatggtattt tagcaaagaa agacgttcac cgattcaata atggtgaagg aagttattat
    63421 agtatcccag tagaaaaaat tatttatacg acagtcaaag tcagagaaga tctagatacc
    63481 aaaaaagcat atgaacctat ttttacaact tattgattga cagcagatgg aggaaaaaaa
    63541 tgaatccata tgaacaactt attcaagatt tgatcgatgg aaaaatcgaa aagattgatg
    63601 taaaaagaga agaattaatg gcttttcgag aagcgtggct gaagttggag aatcgtaaat
    63661 atgtggtggg aaaagcctca ctgaacggca atgtgactta tcattacgat ccgacacgtt
    63721 tattttagta tgaagtattc agaaaagaac caaaggagga cgatgcagca gcattgtcct
    63781 cctttgattt tacaaaagtg aaagttttac ttatttgaat agatttttaa atttgttttt
    63841 gacactgtcg atatttacat cttttaaaag attttgggct ttctctttat aaggtcctaa
    63901 ttgttcttta tgttcttcga taaatcgttt tgcgccatcg aaattgccgt tcttaatcat
    63961 ttccttgact ttttgaaata attcttcttg attcattttg aacacatcct ttgctatcaa
    64021 catgattttt tacatggaca aaacgagaaa aactgattta tccacacgtc aataatatca
    64081 aataacgatc atttttacta aaaaaacact cgattctcgc ttctaaagta ggagaaaaat
    64141 ctccaacttg ggacggatgg aatttcttta actgacccat atactgaata tataacaagg
    64201 aggtagaaag atgaaagtga ttaaatattt gattggactg gcggcgatta gtggtatttt
    64261 gctatttgga ctgaaatatt atactgaagg aagttatgga gaaatccctg gtatacttga
    64321 tcagctaaat ccattagttg aaaaaggaga ggtctatgta aagacacaaa aaccagagga
    64381 agtaaatgaa tatggtacag caaggtatgt gcagaaagct gctgatgcca atgggaaaga
    64441 acgaacggtt gaatttaatg gtctttctgt gttaaaagaa aatcattatt tgaaaattag
    64501 caataaagga gcacacatag aaacgtatga ggaagttcct agagaaaaag ttccggagaa
    64561 agcattagca attattgggt aaatcagttt ttgtattaag aatacgcccg attttattat
    64621 tcataattta attatgtaaa ctaaatacaa gtagtaaaaa taatacaata atccccctgc
    64681 aaaaagattg ttcttttaca gggggttcat tttttcagca agcacatcaa attgggatgg
    64741 cagggttcga acctacaatt gcttgattca gagtcaagtg ccttaccagt ttggctacat
    64801 cccatttatt caacaaaata atcatagcac tttcctaaaa aaacgcaagt tatttttctt
    64861 gatcggatag ttgttgcttt actcgtaaaa ggcggcgtac tagtttcttt tctaatccag
    64921 caggaagggc aatcagttct gctactgaca agacggtgtc caataactga tattctttga
    64981 caggttcatt cataatatga tagatgtttg ctaaacgttc aatatattca aagcgagtat
    65041 actctgttcg agcaattaga aatttcaata aatcggtggc tttctcgtac tcttgatctg
    65101 cgtaataaac aagagattga cgatacaatt tgatttccag ttcattgagg gtagcaggtc
    65161 tgcgacgctc gttccactca ttaatagaga ctttaccttg aacatttccc ttgcagtcac
    65221 ggacaaaaat aatcggaatt tcatttggat ccattttttt atgtgtgaat ttttccatta
    65281 tctacgctcc cttcaaaggt ttggtatctt gagtataagg gatttttgtt tgaattacta
    65341 gcttttctcc aaagttagca tctgtccacc cattgcagtg aaaatcgccg aaaaatcgtt
    65401 gatcggtact gctcgatttt ttacaccata gggatcgttg acataaatat tttctttgtc
    65461 atatccagtg actacacaag catgaacaag aggagtgact cttacttctc cattatttgt
    65521 ggtccaagtc ataaaatcag tttcatctgg gacttcaaaa ttgatagtca cagaagtcca
    65581 gacaggattt cctttagata gtgctttttt gatatcatcg agttgagcat cggtttttgg
    65641 aaaagtgtga taatccgttt ggacgatttg ctgagcaagg gaggcaactg ggggaacata
    65701 gacgaccata gcccagtctc cgccactgat atcaccaaca aatccttcat taggatcgcc
    65761 atgagtccca tctgcattga aaacagggac gtaatccaat tgctcagcta actggttttt
    65821 agttgtttca taaccataaa aagataaaag catagaaagc gaagtgactt cacagccatt
    65881 ttctaaagaa ggagaatcaa actggctctc taatggaaca tctagtaaaa cttctgcttt
    65941 ctcttttgtc tttttcgttt tttgtgttga acttttttgt gcagtgttca ttgtatgttc
    66001 tggagaatgg atatgtaggt aataacttgt accgcttaat aaaagagcag ctgtgccccc
    66061 tagaaacaat agttttttga acgttttttt cttcat
//
LOCUS       DACDPS010000006        65377 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.006, whole genome shotgun sequence.
ACCESSION   DACDPS010000006 DACDPS010000000
VERSION     DACDPS010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 65377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.006"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..1522)
                     /locus_tag="IWJ07_000442"
     CDS             complement(<1..1522)
                     /locus_tag="IWJ07_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332048.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYE"
     gene            complement(1620..1895)
                     /locus_tag="IWJ07_000443"
     CDS             complement(1620..1895)
                     /locus_tag="IWJ07_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9332049.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            2127..2861
                     /locus_tag="IWJ07_000444"
     CDS             2127..2861
                     /locus_tag="IWJ07_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9332050.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            2900..4042
                     /locus_tag="IWJ07_000445"
     CDS             2900..4042
                     /locus_tag="IWJ07_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9332051.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(4137..5528)
                     /gene="sufB"
                     /locus_tag="IWJ07_000446"
     CDS             complement(4137..5528)
                     /gene="sufB"
                     /locus_tag="IWJ07_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAP9332052.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(5533..6003)
                     /locus_tag="IWJ07_000447"
     CDS             complement(5533..6003)
                     /locus_tag="IWJ07_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAP9332053.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(5990..7225)
                     /locus_tag="IWJ07_000448"
     CDS             complement(5990..7225)
                     /locus_tag="IWJ07_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP9332054.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(7222..8508)
                     /gene="sufD"
                     /locus_tag="IWJ07_000449"
     CDS             complement(7222..8508)
                     /gene="sufD"
                     /locus_tag="IWJ07_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAP9332055.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(8521..9294)
                     /gene="sufC"
                     /locus_tag="IWJ07_000450"
     CDS             complement(8521..9294)
                     /gene="sufC"
                     /locus_tag="IWJ07_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAP9332056.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(9491..10339)
                     /locus_tag="IWJ07_000451"
     CDS             complement(9491..10339)
                     /locus_tag="IWJ07_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9332057.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(10363..11049)
                     /locus_tag="IWJ07_000452"
     CDS             complement(10363..11049)
                     /locus_tag="IWJ07_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9332058.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(11042..12076)
                     /locus_tag="IWJ07_000453"
     CDS             complement(11042..12076)
                     /locus_tag="IWJ07_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9332059.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            complement(12471..12809)
                     /locus_tag="IWJ07_000454"
     CDS             complement(12471..12809)
                     /locus_tag="IWJ07_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAP9332060.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(12811..13167)
                     /locus_tag="IWJ07_000455"
     CDS             complement(12811..13167)
                     /locus_tag="IWJ07_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAP9332061.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(13173..14360)
                     /locus_tag="IWJ07_000456"
     CDS             complement(13173..14360)
                     /locus_tag="IWJ07_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP9332062.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(14551..15396)
                     /locus_tag="IWJ07_000457"
     CDS             complement(14551..15396)
                     /locus_tag="IWJ07_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9332063.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            15630..16595
                     /locus_tag="IWJ07_000458"
     CDS             15630..16595
                     /locus_tag="IWJ07_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAP9332064.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            16972..18015
                     /locus_tag="IWJ07_000459"
     CDS             16972..18015
                     /locus_tag="IWJ07_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAP9332065.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(18077..18541)
                     /locus_tag="IWJ07_000460"
     CDS             complement(18077..18541)
                     /locus_tag="IWJ07_000460"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAP9332066.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            18787..19734
                     /locus_tag="IWJ07_000461"
     CDS             18787..19734
                     /locus_tag="IWJ07_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9332067.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(19945..22017)
                     /locus_tag="IWJ07_000462"
     CDS             complement(19945..22017)
                     /locus_tag="IWJ07_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9332068.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21950..22327)
                     /locus_tag="IWJ07_000463"
     CDS             complement(21950..22327)
                     /locus_tag="IWJ07_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAP9332069.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(22456..23016)
                     /locus_tag="IWJ07_000464"
     CDS             complement(22456..23016)
                     /locus_tag="IWJ07_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9332070.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            23166..24458
                     /locus_tag="IWJ07_000465"
     CDS             23166..24458
                     /locus_tag="IWJ07_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9332071.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(24518..25636)
                     /locus_tag="IWJ07_000466"
     CDS             complement(24518..25636)
                     /locus_tag="IWJ07_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAP9332072.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(25650..27071)
                     /gene="arcD"
                     /locus_tag="IWJ07_000467"
     CDS             complement(25650..27071)
                     /gene="arcD"
                     /locus_tag="IWJ07_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAP9332073.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(27317..28438)
                     /gene="mnmA"
                     /locus_tag="IWJ07_000468"
     CDS             complement(27317..28438)
                     /gene="mnmA"
                     /locus_tag="IWJ07_000468"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAP9332074.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            28842..29441
                     /locus_tag="IWJ07_000469"
     CDS             28842..29441
                     /locus_tag="IWJ07_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAP9332075.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(29579..30865)
                     /gene="efmA"
                     /locus_tag="IWJ07_000470"
     CDS             complement(29579..30865)
                     /gene="efmA"
                     /locus_tag="IWJ07_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAP9332076.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(31136..31480)
                     /locus_tag="IWJ07_000471"
     CDS             complement(31136..31480)
                     /locus_tag="IWJ07_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAP9332077.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(31617..32753)
                     /locus_tag="IWJ07_000472"
     CDS             complement(31617..32753)
                     /locus_tag="IWJ07_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP9332078.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(32942..33916)
                     /locus_tag="IWJ07_000473"
     CDS             complement(32942..33916)
                     /locus_tag="IWJ07_000473"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP9332079.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(33996..34871)
                     /locus_tag="IWJ07_000474"
     CDS             complement(33996..34871)
                     /locus_tag="IWJ07_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAP9332080.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            35001..36908
                     /locus_tag="IWJ07_000475"
     CDS             35001..36908
                     /locus_tag="IWJ07_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAP9332081.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(37449..40727)
                     /locus_tag="IWJ07_000476"
     CDS             complement(37449..40727)
                     /locus_tag="IWJ07_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAP9332082.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(40998..41543)
                     /locus_tag="IWJ07_000477"
     CDS             complement(40998..41543)
                     /locus_tag="IWJ07_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9332083.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(41555..42247)
                     /locus_tag="IWJ07_000478"
     CDS             complement(41555..42247)
                     /locus_tag="IWJ07_000478"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAP9332084.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(42266..42550)
                     /locus_tag="IWJ07_000479"
     CDS             complement(42266..42550)
                     /locus_tag="IWJ07_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332085.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(42563..43126)
                     /locus_tag="IWJ07_000480"
     CDS             complement(42563..43126)
                     /locus_tag="IWJ07_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP9332086.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            43354..44016
                     /locus_tag="IWJ07_000481"
     CDS             43354..44016
                     /locus_tag="IWJ07_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAP9332087.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            44140..45336
                     /locus_tag="IWJ07_000482"
     CDS             44140..45336
                     /locus_tag="IWJ07_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAP9332088.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(45437..46813)
                     /locus_tag="IWJ07_000483"
     CDS             complement(45437..46813)
                     /locus_tag="IWJ07_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9332089.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(46850..47716)
                     /locus_tag="IWJ07_000484"
     CDS             complement(46850..47716)
                     /locus_tag="IWJ07_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAP9332090.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(47805..48830)
                     /locus_tag="IWJ07_000485"
     CDS             complement(47805..48830)
                     /locus_tag="IWJ07_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9332091.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(48848..49522)
                     /locus_tag="IWJ07_000486"
     CDS             complement(48848..49522)
                     /locus_tag="IWJ07_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9332092.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(49706..50599)
                     /locus_tag="IWJ07_000487"
     CDS             complement(49706..50599)
                     /locus_tag="IWJ07_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9332093.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(50673..52370)
                     /locus_tag="IWJ07_000488"
     CDS             complement(50673..52370)
                     /locus_tag="IWJ07_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP9332094.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(52363..53676)
                     /locus_tag="IWJ07_000489"
     CDS             complement(52363..53676)
                     /locus_tag="IWJ07_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP9332095.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPQAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESSEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMDELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(53676..57713)
                     /locus_tag="IWJ07_000490"
     CDS             complement(53676..57713)
                     /locus_tag="IWJ07_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP9332096.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFYTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVYETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(58482..59000)
                     /locus_tag="IWJ07_000491"
     CDS             complement(58482..59000)
                     /locus_tag="IWJ07_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP9332097.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(59002..59619)
                     /locus_tag="IWJ07_000492"
     CDS             complement(59002..59619)
                     /locus_tag="IWJ07_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP9332098.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(59616..60470)
                     /locus_tag="IWJ07_000493"
     CDS             complement(59616..60470)
                     /locus_tag="IWJ07_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9332099.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(60467..61378)
                     /locus_tag="IWJ07_000494"
     CDS             complement(60467..61378)
                     /locus_tag="IWJ07_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9332100.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(61419..62753)
                     /locus_tag="IWJ07_000495"
     CDS             complement(61419..62753)
                     /locus_tag="IWJ07_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9332101.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTYTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            62961..63791
                     /locus_tag="IWJ07_000496"
     CDS             62961..63791
                     /locus_tag="IWJ07_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAP9332102.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(64035..64284)
                     /locus_tag="IWJ07_000497"
                     /pseudo
     CDS             complement(64035..64284)
                     /locus_tag="IWJ07_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(64475..64669)
                     /locus_tag="IWJ07_000498"
     CDS             complement(64475..64669)
                     /locus_tag="IWJ07_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332103.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSGF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(65174..65359)
                     /locus_tag="IWJ07_000499"
     CDS             complement(65174..65359)
                     /locus_tag="IWJ07_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332104.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
BASE COUNT        18790 a        13884 c        11620 g        21083 t
ORIGIN      
        1 attcataaga aaaatattga tatgtaaaat gaattttttc cagaagtgga aggatatctt
       61 cattcaattt ttgaatcaca gaagttgcat taaaaacgtc taatcgatag acattgcttc
      121 tcttatgatt aaagtttcct aacgctttct cttcctttaa cctatttagg tattcgagca
      181 tcttcaaagg aacatcttca tcatctaatt tttttatcaa tcctataaaa gtcataagtg
      241 atagtaactt attacattta tctatgtcat aagttttacc atagtaagaa ctcatttttt
      301 tagctaattg ttcccccgac aaaatacaag tcacactttc cttactatca tcaaaaaaat
      361 taacatcaga aagcaagaca tcatagacac gtcgcatttc ctctagataa ttccttattg
      421 tcctgaaaag tttgtctttc attgattcaa gagtgtcaaa aagtactttt gattcctctt
      481 tcaattttcg aaaatgtgta ggatacaagt ctaggttctc taatagccaa ttaaatgttt
      541 ctacccgact gtctttcttt aaaattagca tcacaactcc aataaaattt tcactgatat
      601 cacaattact ggaatgacaa taaaaaatgg aatgcccagt tttactggta aaaatactag
      661 cagagggatt atgatcgttg tgaaacagac atcttaagtt tttacttgac ttaagtaatt
      721 tattcatatc aatggttaac aatctttcat aaatctcatt aaaagacatg tcggaggaat
      781 caaacaggca gtctttaaac cactgtttca tttcttcttt gtcgtcattt tttatcaact
      841 taacaaaatc ggtttcattg actagttcat actctctccc tgaatcactt tgacaatttt
      901 tagaacatgt catggtaaaa tcaatcggaa gctcagtaat atctaacatg ttgtcttcat
      961 taattattat cactgcatct gacttatatc ctccaaaaaa cattctattc gaagcattta
     1021 cattgtcatc acacccgggg atgttctttt tcaagtattc gtaaacagca accattttac
     1081 catagtcagt aattgcttct tttaatagaa agacaatctt gtatttttct agattttcag
     1141 agtaccgaat acttttttgt atcgcaaacg cagaattatg aactaaattg atagataaaa
     1201 ccttttctat gcttaagtat ctactatcat ctttactcaa atctactacc ttccccttct
     1261 catctttaac agtgttatcg acatccagaa taattactct ggtttccaca aaactctcct
     1321 tgtcattgcc tttattcttt gtcttgcttg gaataaatga ttgtgatgaa tttagtatct
     1381 ctacaaactc atctcttgta acttctctat agttatctaa acacctatct cttacatcag
     1441 ttttttcttt ttttgtaact ggcttctcta ataatttttc ttgatctaca taaattggaa
     1501 tattaatcat tgatgtttcc atttgtgttt cctccttata aaattctgtt aaagactaaa
     1561 acatacagat aataaaaaca aactctttat tactatatgt tttagtctta tttgtttact
     1621 caacctttat accgttccat gaaactatca atatcagatt ttttaaatct aatgaccgta
     1681 tcaattttta taacaggtaa cccgtattga gaaacatatt ttctaatcgt tcctactgat
     1741 acacctagat attcacttgc ctgctgatag tccatatact ctagagtgac tccagaaata
     1801 atgtcttcta gcataggctt tagcactgta attatctctt tcacttgttg ttcaacttgt
     1861 atagaaacaa tgctatcaat gatttgtaga gacatatttc cccctccttt cgtgattgct
     1921 tgaacaaatt ttctaagaat tatcttcgta ttttttgctc aaaataaaag tattttgtta
     1981 ttaaccgatt aatcaaacaa aaaatgatta ccagttcatc gacatacaca taatacctcg
     2041 ataaaaaagg ggtgtcaaca gctttgtaaa atttatttac aacacccctt ttttgaggta
     2101 tactaatttt aggaggtgtg gtcaatatga taaaacgtcc accaataaat tatttagaac
     2161 gaaaaaagat actcggaaca aaaataaaag ctataagaaa gagtaaaaaa ttaacacaac
     2221 cagcatttgg tcttatgatt aacaacggtc aattaattga caaaaaaact atctacgaat
     2281 gggagaaggg aacctattta ccaataccag agcgattatc ccgaattgct gatttgggaa
     2341 atatgagtat agaggagtta gtttgtggca atgtagaaga atatattttg ggaattattc
     2401 tttatagaga tagcattgta ctagatggta taacatttcc agataaaaac ctgttccagc
     2461 atcttcgtca acagttccca ccagtacact ctaatttaga tacatggctg gatagatatt
     2521 ccaaattaga accagagatg caagaattta ttgctaacaa gacttgtaat aaagttaaaa
     2581 atgaaaaaat tagtcttttc aatatcttaa aaatagaaga attattcatc aatgctattg
     2641 tagaagaatt tgacaataac attcttttcc tcactagcag tatagaagaa ctactagaaa
     2701 gaatggtaga tgaatggcta cctattcaat tgaaagacat gtcttatccc gaagaagctg
     2761 tgagagaaat tactgataat ataaacaagc tagaacaaac gatttcatca attgggaaaa
     2821 aatacactaa gaaaaaaatg aagggaggtg ataccatcta gtggtaagta ctccttgtaa
     2881 actaagagga gatgatttaa tggcaaaaat atcaaagtac aaaaagaaaa acggtcaagt
     2941 ggcgtggatg tataaaggac acattgccac cgatccacga actggtgaaa aaataaatac
     3001 tacgagaaga ggttttagta gcaaacaaga agctcaaaaa gattatgacg agtacagaca
     3061 tcaaatatta tacggtgtta aaaagaaagc acctgatatg acatttgaag acctgtataa
     3121 tgaatggata actcatcagc gtaccagtgt taaagcttca acaatcgcaa tctcagtaag
     3181 atacgcaaat aatcaaatac ttccagcatt tggtaagctt aaactatcga atatctctgt
     3241 cccatattgt caaaaagtgg tagatgaatg gcacagtaaa tatgaaagtt acgattatat
     3301 gagaaaacag actgcacaaa ttttacgcta cggggttgca atgcaatata ttgacaataa
     3361 tcctatggaa aaaacacttc ttccacgaaa aaaagagtat gagaaaaatc gtaaatttta
     3421 ctcaaaagag gagcttaata atttactgga tgcttttaaa gattttggta acatgaaaca
     3481 atacgccttc ttcagattat tggcatacac aggtatgaga aagagtgaag tactcgcttt
     3541 acagtggaaa gacatagaca cgtttaataa agaactacat gtcaataaaa ctcttgctgt
     3601 agatgaattt ggtaaagtta taattcaatc tccgaaaaca agagcttcta gacgagttat
     3661 ctctttagat actgagactc tttctatcct caacaactgg aaattacagc aaaaagaaga
     3721 gtatttaaaa cttggataca atactagctc taaagagcaa catgttttta caacagttaa
     3781 aaacactcta tacattccaa acactgttaa tgattggtta agatatattt tgaaaaaata
     3841 taacttgcct cgtattacac ctcatgggtt tagacataca catgctagtt tattattaga
     3901 agcaggagaa tctgtcaagg tagttcaaca acgattaggg catgaaaata gtaaagtgac
     3961 attagacatc tatgcacata ttacaaataa tgcacctaag aaaacaggtc aagactttgc
     4021 tgatatgatg gctcatcagt aaaagagctg gtcaaaatct ggtcatttct agtatacagg
     4081 caaaacaaaa aagctagaac ccttatatat caagggttct agctcctata acaggtttac
     4141 ccaacactgc cttccatctc ataactgatc aatcgattga gttcgactgc atattccatt
     4201 ggaagttctt tggtgaatgg ttcaacaaag cccatgacga tcatttcagt tgcttcagat
     4261 tcggtcaggc ctcggctcat cagatagtag agttgttctt ctgagatttt tgagactttg
     4321 gcttcgtgtt ctaaagagac ttggctgttg tggatctcat tgaatgggat cgtatcggat
     4381 ttcgaccatt cgtccatgat gatcgtgtca cattcgatat gagagatcga acccgcactg
     4441 tttttggcaa aggttacttg tcctcgatag tttacttctc cgccgtcttt tgaaattgat
     4501 ttagagacga tagagctaga tgtatttggt gcattgtgga tcatttttgc acccgtatct
     4561 tggatttggt ttgctcctgc aaaggcgatg gagagcatgg tgcctcgtgc accttttcca
     4621 tctaagtaaa cacttgggta tttcatcgtt gtttttgcac ctaggtttcc gtcgatccat
     4681 tcaacggttg cgccttcgta tgcttttgct cgtttggtta ctaagttgta cacattgtct
     4741 gaccagtttt ggatcgttgt gtaacggcaa tacgcatctt ttcttgtaaa gatttctacg
     4801 atggcggcat gcaggctgtt acttgaataa gtaggtgccg tacaaccttc gacatagtgg
     4861 atgcttgctc cttcgtcaac aatgatcagc gtacgttcga attgccccgt gttttccgca
     4921 ttgatccgga agtacgtttg taatggaaca tcgactttga cgccttttgg cacgtaaata
     4981 aaggtcccac ctgaccatac ggctgaattc agcgctgcta atttgttgtc ggttggcggt
     5041 acaagttttg aaaagtactc tttgaataat tcagggtatt ctttcaaagc agagtcagtg
     5101 tcagtaaaga cgattcctaa cttttcgaat tcatctttca tattatggta cacgacttct
     5161 gattcatatt gtgcggaagc accggctaga taagctcgtt cagcttctgg tataccaatc
     5221 ttttcaaatg tatctttgat tttttctggt acgtcttccc agtcacgagc gggtttgtca
     5281 cttggttttt ggtaatattt gatggcatcg aaatcgatgt ctgaaaggtc ggggccccat
     5341 gtttgcattg gcattttgtt aaatgtttct agagatttca gacggaattc aagcatccat
     5401 tctggttcgc ctttgattct tgacatctca cggatcactt cttctgtcaa gccttctcct
     5461 gtactgaaaa ccggttcgac atcatcgtga aacccgaatt tatattcttc taattctggt
     5521 acgcccatgt tctcactcct cttccttgtg gtaatgatgg aaagctgcgg taccatcgtt
     5581 ttccattgct tttcctagtg ctttccaggc aagtgtcgca catttgatcc gagcgggaaa
     5641 tttagctaca ccaccaagca tagcagcatc gcctaattgg tcgggatctt tgacttcttt
     5701 tccttggaca agctgtagaa agtctacagt taaatgttct gcttcttcta ttgattttcc
     5761 gatgacggcg tctgtcatca tcgaggcact tgccgtactg atggagcagc cactgccgtc
     5821 aaaggcgata tttttgatga ttccgtcttt tacatcgact tgcaattcga tcacatctcc
     5881 acatgtgggg ttgttcaatt cgatcttgct tgtcgagtca gtcaagcttc cacgatgatg
     5941 cggatgggaa gaatgatcca agatcacttg acggtataaa ttgtctaatt tagatagtcc
     6001 catgttggaa aaactccttt gttgccatga ttgcttcaac taaacggtcg gcgtcttgcg
     6061 ttgtgttgta gaaataaaag ctcgcgcgag cagtcgctga tacttcaagg tacttcaata
     6121 gcggttgcgc acaatgatga cctgctctga cggctacccc ttccatgtct aaagctgtcg
     6181 cgacatcatg gggatgtaac ccatccaaat tgaatgcaac gactcctgtc cgaaaattcg
     6241 ggtcttgtgg accataaaca gtcaatccat caattgcttg taattttggc aacacataag
     6301 cggctagttc ttcttcatat cgatggatat tgtccatacc gagtttttgc aaataatcga
     6361 ttgcatggcc taatgcgata gcgccggcga tatttggtgt ccctgcttcg aatttccaag
     6421 gtagttcttt ccatgtactg tcgtataatt cgacaaaatc gatcatttcg ccaccaaatt
     6481 caacaggttc catttcttct aaccattttc gtttaccata taaaacacca atacctgttg
     6541 gtccgcacat tttgtgtccg ctgaaagcaa agaaatcaca gtctagtgct tggacatcaa
     6601 tggccatgtg cggggcagat tgagcaccat ctactacgat gaccccgccg ttttcatgga
     6661 caagttcagc taattcttga acaggattga agacacctaa gacattggat acatgagcaa
     6721 tggagacgat tttggttttg tccgtaatct ttttcgctgc atcctccata tccaggaagc
     6781 cttcttctgt tagttcgata tatttcaaga cagcacctgt tcgttttgcc aactgctgcc
     6841 acgggataac attagaatga tgttccatgt aagagagcac tatctcgtct cctgctttga
     6901 tatagcggtc gccgaaggtt ctggctaccc aattcaagct tgtcgtcgta ccacgagtaa
     6961 acagcacttc tgctgtttct tttgcatgga tgaacgcccg tactttttct cttgcttgtt
     7021 cataatcatg ggtcgcgcgt tctgctaacg tatggacacc gcgatgaaca ttggcgttat
     7081 cgtgttcgta atagtacgct aatgcctgga gtacttgttt tggcttttgg gtcgtcgctg
     7141 cattatccag ataaactaaa ggttcatcat tgacgtcttg aaataagata ggaaaatcat
     7201 tgcgtagctt tttagcatcg atcatccatt caacttccct tcgattactt cgactaattc
     7261 tttccgaact tgttctacag ggattgctgt taagacagaa ccgaggaatc ctcgaatcac
     7321 taagcgctct gcttcttctt tatgcaatcc tctactcatc agatagtaca tttcttctgg
     7381 atcaactcgt cctacactgg cagcatgtcc agcagttact tcgttttcat cgatcaaaag
     7441 aataggattc gcatcaccac gtgctttatc agaaagcatc aagacacgac tttcttgttg
     7501 ggcatctgca ccttttgccc ctttcaagat gtggccgata ccattaaagg tcaatgttcc
     7561 tttttctcgg atcactccgt gttgcaaaat atgcccgatc gtatgagggg ctttattggt
     7621 tactcgtgta tcgattcctt ggatttgtct gcccgagctg attgccacga ttttgacttc
     7681 tgcgtgtgca ccttctcctg ctaaatctga gtcaaagtca gcgatcacat gaccgtcatt
     7741 catgacacca attgcccagt cgacagaagc atctcgtaaa atgtgcccac ggcgattcat
     7801 ataagaagta atattttctc ctaattgatc tacggctgaa tatttgattt ttgaaccagc
     7861 tttagcgatg acttccacga tgatattacc tgatgatttt gctacttgtt ctttggttgt
     7921 ctggaaacgt tccagataag aaaattcact atgttcatct gccacgatca acacatgctt
     7981 gaagaagtgc tcatcggatg cgccatcttg aatgaataag gactcgagtg gttcatcgat
     8041 cacgacattt ttaggcacat acaggaaaat accgctgttc ataaaagcta catggagtgc
     8101 agtcaattgg tcttcgttca tgtttactgc ttttttcata taatattctt ggactagttc
     8161 tggatattcg atcattgcag tgaacaagtc ggtaaaaatg actccttttt ctgctaattc
     8221 cacaggcagt tgttcaaaaa tcgtccatga accttgttgg acaagcacag gattgtcttt
     8281 cattgcatca aatgccggaa tcgtgcctgt ttcactgatg gtttgtgttt ctttcacatc
     8341 aaataacgac cagcgatgga atttcacccg atcgatatgc ggcaatggca gttcatctgc
     8401 tttcgctagt gcgtcttgac ggaaggttct catccaatca ggttcgcctt tacttaaaga
     8461 aaaatcattt accgcgtcaa gataatcgag ccagtttttt tctttcattc ttgctccccc
     8521 ttacgcttct tcttctttat agtcgattcc taattcttta ctgataccag cgtagccttc
     8581 tgcttccaga cgtttagcta ggtcagcatt ccctgtcatg actactcggc cttccatcat
     8641 gatgtgaacg acatcaggtg tgatatagtt caataatcgt tgatagtggg tgatgatcaa
     8701 tgcaccgaaa ttgtctccgc gcatttcatt tactccttta gcaacgactt tcaaagcatc
     8761 gatatccaga cctgaatcga tttcgtctaa aattgcaaat gtaggttcta acatcaacaa
     8821 ttgcaggatc tcattccgtt tcttttctcc gccagaaaaa ccttcgttca gataacgttc
     8881 agccatttct tctggcatat ttagtaaagc cattttttca tctaactttt tcaaaaatgc
     8941 cataacagaa attttgtcgt tttcatcacg tttcgcattg atcgctgcac gcatgaattc
     9001 cgcattcgtg atacctggta tctcacttgg atattgcata gctaaaaata agccaagtcg
     9061 cgcacgttca tctacttcca agtctaaaac atttttcccg tcaaacaaaa tttcgccttc
     9121 tgttacttca tagtttggat tgcccatgat agcggcagat aaggtagatt ttcctgtgcc
     9181 gttaggtccc atgatggcat ggatttctcc ggttttcatt gttaggttta cgcctttaag
     9241 gatttctttt tcctcgatcg acacatgtag attttttatt tctaagacag acatgcatta
     9301 tacccccaaa ttctctttcg ttctcactca ttttagacta attgagaatg aagcgcaatg
     9361 accttcctta ctaaaatcaa atatctttta tttattatta aacaattttg atttctaaat
     9421 tagaataggt ataaaaaaga tgagaaataa gccattacag acttatttct catctttttt
     9481 gttccatacc ttattttgca tcagccttta ctggtttaac agagccgccc catttgtctg
     9541 tgatccattc ttggacttct ttgcggtgaa gcacttcaat cagttttttg attttgtcac
     9601 tgtcttcatc acctttacga gtcgcaataa tattcacgta aggagagttg tccttttcaa
     9661 gcaatacggc atcttctaat ggattcaacc ctgcgccgta agcgaagttt gcgttgattg
     9721 caactaggtc accttcatca ttttggtacg tagaagctaa aagtgatggg tcgatcgtat
     9781 ggttgaactt caagttctta gggttttctt caatatcatc gaatgttgct gtttccagat
     9841 ctactccatc tttgactttg actaagccag catcttgtag aattgtgatg atgcgtcccc
     9901 agtctgattc agaatttgaa gtaatgattg ttgcaccatc ttttaattcg gaaacatctt
     9961 tgactttttt agagtacaag cccattggtt cgatgtggac agcaccggca ttgacaaagt
    10021 catatccttt ttctttgatt tctttgttca aataaggaat atgctggaag taattggcat
    10081 caatgtcgcc ttcttccaaa gattggttcg gtaatacata gtcatcgaat ttgacgattt
    10141 caagatcgat tccttcatct ttcaataatg gtttcacatg ttccaaaatc tctgcgtgcg
    10201 gtgtcggtga agcgccgact ttcaatgtcg tgtcgtctgc tttatttgct gaatctttgc
    10261 tgtcgctgtt gttaccgcat gctgctaatc ctacagtcag caaaagcgct gctgcaaatc
    10321 caaaaatttt ctttttcata tcctacatcc ccctatatcc tattatcttt tgtctgtttt
    10381 cttagttaag aaatcaccga ttccttgaac gataaatacg attagtaaaa taattaaagt
    10441 cgcaacgaat gtcactgtta gattaccacg ctggtagcct tcctgccaag caagacctcc
    10501 aagtccacct gcgccgatcg cgccggccat cgcagtaaat ccgatcatcg agatcgttgt
    10561 aactgttaat ccagaaatca atgcgggaat actttcaggt atcaagacct tccagatgat
    10621 ttcccagtaa cttgctccca ttgcatctgc tgcttccagt acaccagggc tgacttcacg
    10681 aaaagcaatc tctactagac gggcataaaa tggtgctgcg gataaaacaa gagctggaat
    10741 ggctgcttgc gcacctagca tagtgcctac gattgcttta gtaaatggaa tgatcaaaac
    10801 catcaggatc ataaatggcg ttgaccggaa aatattactg atgatcgata cgattccata
    10861 aatgattcga gcatacggtt tgtttttgcg tccaatagag taaaggatca atcccaaaac
    10921 taatccgatg atcgtcacta agaccattga gattaatgtc atatatagtg tatcaaggat
    10981 agcctgattc atcgtttccg ggttgatttg tttaaaatct agatatgttt ctgaaaagct
    11041 tttatccacg atggatcacc tcaattccta ctccttgttc tttgatgaac acttctgctt
    11101 gatctaattc tttggtttca ccagtcatca agacagtcaa tgaaccaaag gaaccttctt
    11161 tcacttgttt gatctttcca tacacaacgt tgacatcgat gttatagcgt cgaatcgctt
    11221 gggaaatgac tggctcgttg gcatttcttt cgctaaaggt caaagttgcc aaacggccgc
    11281 ttggattttc tttgatgagt tcagctaata gttctgtcga atcagcttct ggttcgatgt
    11341 cttgttgtac aaagcgtttg gttacttctt ttttcggttg tctgaagaca gtcaatacgt
    11401 ctccttcttc tacgactttc cccaactcca tcacagcgac tttgttgcag attttgcgga
    11461 tcacattcat ttcatgtgtg atcaagacga tggtcaaatt caatttctta ttgatcgcta
    11521 acaataagtc taatacttct tctgttgttt gcgggtccaa tgcgctagta gcctcatcac
    11581 aaagcaggat ctcaggatca tttgccaatg ctcgtgcgat accgacgcgt tgtttctgtc
    11641 cgccggaaag ttgtgaagga tatgcttgtc cgcgtccttc taacccgact aaacgcaaca
    11701 gttcttctgc ccgttcttta cgtttgctct ttggtacgcc agctaattcc agcggcagct
    11761 ggatattttc aagtacagtt cttgaccata acaggttgaa atgctggaag atcataccga
    11821 ttttctttcg gaatgtacgt aattctttgt tttttaattt tgtaatatct tgatcgttta
    11881 cgatcacttc gccgctcgtg ggaagctcaa gtccattcag gagacggact aatgtacttt
    11941 ttcctgcgcc agaatagccg acgattccat aaatatcccc tttttcaacg tttagtgaaa
    12001 cgtcattgac ggcttggatg tttccgtttt tcccattgaa tgtctttttg acattcgtta
    12061 gttggattaa tgacatgcca ttcacatctt tctcttatga tttttcaaac aaaaaagcct
    12121 tcgccctctt gccttaagca aaaggacgaa agcttgaact tccgtgttac caccttgatt
    12181 taccgttact tcgcaataac agcctcttcc agtacttgga cttagcttct ttccgtatac
    12241 tgtcgcgctg taacgggcac tcccgtagta agtttgctta cgctcgctta ctctgctcta
    12301 agaccatctt ccatctcatt ttcgttaccc attttcagca aacagggctc tcttcaaacg
    12361 tccgtgacat gtactcttct tttcaaagct ttctcgtttc atataggatt gcttatgatt
    12421 atagcaactg gaagaatcag tgtcaacttt tttcaagtga ttttctgatt ttataattcg
    12481 tcaaattctt ttggatcaac atctttgaaa cttaagatcc acgcattcat ttcatctgaa
    12541 tcattcaaga tattgatatc ttcaccgatt ttatcattaa cagaagaaac tgtacccgtt
    12601 agcgggctgg caaattcatt gaccgctttt tccgcttcaa cttcgatcag aggttcatct
    12661 ttttttagcg attgtcctgc ttttggcaga ttggcaaacg tgatgtctcc aagttcttct
    12721 tgcgcttcat ttgtcaaacc aacacagtat tcttttccat taaataagat ccaaaggttg
    12781 tcctttttct tcaaatgctt gcctgccatt ttatatcagt tctccttcat aaattttttc
    12841 attgaatcca attgcaacaa gctggtcctc tcgtaagatc agtggtcgct tgatcagcat
    12901 accatctgtc gataagactt ggctcgcttt tgaagccgta aactcgccta cttgatcttt
    12961 cagtccaagt tcccgatatt tcatcccgct tgtgttgaac aggcgacgga ttggtacttg
    13021 actcgtttct agccaagtct caatcgttga ggccttcggt acttctttga tcatatcaac
    13081 ggtttgataa ctaacgccat gctggtctaa ccacgctttg gcttttttgc atgttgaaca
    13141 tttcgggtac cagtaaaagg tgtacaattt tcttacctcc ttgaagatct tgcaggtgct
    13201 tcattttggt atctcatcga taatatcaac ccgataccga tcatgttccc taaaatcgag
    13261 gatcctccct gactaatgaa aggcaatggg atccctgtta aaggaagtaa tccgatattt
    13321 gctccaatat tctcaaagac atggaacagc agcatcatga tcaaaccgct tgcgatatag
    13381 gcgtaaaatt cattattcgt atcgaaacac acccgaatca tacgatagat taaaataaaa
    13441 tacaagaaaa tgacgaatgc accaccgata aagccgaagt tttcaccgat aacggtaaaa
    13501 atcatatcgg actcacgaac aggcacatag atattgctta cgttgaatcc tttaccaaac
    13561 aagccaccgg taccaattgc caaaagtcct tgggccggct gataactcat tcctgacgta
    13621 tcatggaaag ggtccagcca tgaatcaata cgtgcaaact gataagattt gaatccaact
    13681 ttataaagca agtctcttcc gccttcagtc gtaaccaaga agatcgttcc tgctccaacg
    13741 actactgcca agattactac aggtgcgatg atgcgccaag agattccaga catcaggaaa
    13801 atcccgccaa agatcgcaag aaagacaagc atcgttccaa agtcatcttg ggccatgatc
    13861 aatgctaaaa ccggtattgt cacgatcaag agcttcccaa tcagccaaaa gtccgatttc
    13921 atcgtccgtt cttttacttg cgtattatgt ttagttacga tcagtgccat catcaagatg
    13981 tacgcaatct tcatcaactc tgctggctgg aaagtaaacg caccgaatcg aaaccagttt
    14041 ttagaccctg ttgaagcagc taaattctga tcatagaatt tgagcaagag tcccattacc
    14101 actaacccca aaccataaag gtaaggagtc agtttccaga tccatttgga attcaaatgc
    14161 atgatgacaa cgattgctgc cgctccaact aaataccaga caacttgcat ccctacgcct
    14221 ttcaagactg ctggattgtt cggatcatgg ctaagtgcta catataatga gagcagacca
    14281 atcaaacata acagaaagac tggtaaaatc acgccataat caatacgatt atcgttcgaa
    14341 gcttttaatt ttttttccat gtttcatccc ttttcttaaa aaccatactg ctgattatta
    14401 tagcggtcca gttagataat ggaaagtctt tccatagaca tttgggaaaa attaactttc
    14461 cgtttgtgca gttgcttgag caaatgcagt acaaaagcct actgcttatc tttagtgtat
    14521 catagaactt tttttgatac atgcaattat ctaccagctg agattctcct cgctgtgaga
    14581 agtttctgtg ctttcattgt atcgaaggat ctcttcggca atctctagca gctgatcaga
    14641 aaccagattg gttcgttgga tgaaataaaa atatcgatgg aaattcaacg gagtcattgg
    14701 cacattttct ggaacagagc gcttagacaa aatcgagcga ccgattcctt ccttcagcaa
    14761 cgcaacaatt acatcgttac ttttcacgat catcttctta tcttggatat tcatctcttc
    14821 aaaataacgc tgcgtgtaat gaaaaacacc agagtttgct tctcttacaa gccacaattt
    14881 gctttttggg tccccagcga tgactagctg gtcttccatc aaagagtatc tttgtacgcc
    14941 ggaagtctct aatggctttt cgataaaacc aaaatcggat tcatgtttcg ctacttgttc
    15001 taatgattca tgagaattcg tgatttcgat ctcaaaatgc actttaggaa accttttgat
    15061 cagatgtttc atcaactgag gcaaaagata gacagcaaag gtgtgggaag caacgatccg
    15121 acatacttct tcttcatttt gttcattgag caatttttgc tgagtttctt cccattcttc
    15181 taacaaaacc agtgtccgtt catataataa gtcggcttgc ttagttggaa gcatctcttt
    15241 tcttccgtta cgttgaaaaa gatcgatatt caattcccgc tccaactgtt tgatttgatt
    15301 cgagactgct ggttgagata aaaacagtac ttctgctgct ctggaaaaat ttcttgtttc
    15361 atatactgct cgaaaagtcg ttaataactt aaacatggca ccacttccat aactttttat
    15421 gataattact ataaaaacta tttgatttaa atatacaaaa aaccatctat aatagaaatg
    15481 tacaacaaaa cagttgctga tccaactagt aacttagatg aagacaatca gctgtctgct
    15541 tgctcgtaat catacattcc tattcatttt acaatgtatt cgttcatctt ggtactaaaa
    15601 tctattaaat aaaaaggaga atattatata tgaagaactt tttgaaaatt attccgatcc
    15661 cgatctgcgg gttgatcctt gggttagctt cccttggaaa tctgtttaaa gactatcacc
    15721 atgttgcttt aggcaatcta actggcggta tctctatgat tcttatgata ttcatttttg
    15781 ttaaattgtt tatcttattc gaacacacga aacaaacgtt gcatgatcca atcatcgcct
    15841 ccgtttcacc aacttttacg atgtcactca tggttatttg tacttatttt gtttcatatg
    15901 aaagtattgc accaattgtc aaattcattt ggctgtgcgc tgttatcttc caagtgatcc
    15961 ttgtctttta tttcaattat caccatgtta tcaaagctga tctttcaatt gaagcaattt
    16021 atccaagctg gtttatcatt tacgttggat ttggggtcat cactgtaact gccgggaact
    16081 tcttcccact tattggaaaa attttctttt ggatttcatt gatctgctat gcgttcttat
    16141 tgccgatcat catcaaacgt gtctttttcg taagaaatat ggctcatccg acacgtccgc
    16201 tgattacgat cgttgcagca cctggttctc tttgcctaac aggttatctt aaaaactttg
    16261 ctgaacctaa tttttatctt gtcgtagcat tgttcgcttt atctcaagta ttgtatttcg
    16321 ttgtgctgtc tatgcttcca cgtttgttca agcttcgttt ttatccaagc tatgcagcct
    16381 ttacattccc gcttgttatc tctgcaacag ctatatttac tactgttcat tacttcaaca
    16441 gtttaggtat ctatagtagc gtattggatt tcttaaaagt tgcggaatgt ctgtttgcaa
    16501 gtgtagttgt cttctacgtt cttgctcatt acgtacgttt cttaattaaa gaacatagca
    16561 aacatggaaa atttgtttct aaaaaaatcg cttaataatc gattcaatag gttgtgaaaa
    16621 agctgttctg catcaagtaa taagaacggc caaacaaaaa cagctgttca tgtttttgta
    16681 ctggttgggc ttattaccgc agatgcaagc ttttgaacac cgtttaccaa gaggtcgtaa
    16741 aagaagtggt cagcttcaag aaataagaag gaatttacga aaattgcttc tcaaattttt
    16801 gtgaaatttc ggcttatttc cgtagaagct acttctggaa caccgtttat tcgaaacaag
    16861 aggtcgtaca agacttttgt cacgacctct tgttcttttg tcaaaatact ggtttagtgc
    16921 tactatgttc gagtatgctt ttagtattta ttgacgaaag gaaccgatca aatgaaatca
    16981 ttgtcacatc caataaaatc tgtaaaggat tcagcggtcc gcgaaatctt acccggttta
    17041 ctgctttctt ttgccatcgc tgccttcagc aggcttcttg ctatttatct gccacagtta
    17101 ggtggagcga ctattgctat tttattgggt attttattag gaaatacttt ttttaaacag
    17161 cctgttctag caatcggcac aaaatttgct gaaagcaaac tattagaatt ctctgttttt
    17221 ttactaggtg caactgtcac agctcaaacg attgctcaaa ttggtgtaaa aggaattgtt
    17281 tttgtgttac tgcaaatgtc agtcactatc atcggtgctt acttgattgg taaaaaactg
    17341 caattcagcg atacttcttc tttgatgatg gctggaggaa acgctgtttg tggttcttct
    17401 gctattgctt caatcgcccc ttccatccat gcgaaagagg aagaaaaagg acagatcatc
    17461 acactagtca atctactggg tactgtgatg atgttgactc tcccttttct tggattacag
    17521 ttgtttggtg gaaatctcat tgaaaaaagt gcacttttgg gtggtacact ccaatctgtt
    17581 ggtcaagtag tcgctggggc aagcttactt gacaattctg ttgtcgaatt cgccatgcta
    17641 tttaagattc tacgtattat gatgctcgtt gttgttgtct atctgtttga aaaattcatg
    17701 cacaagaaac aaccagctct gtttgaaacg ccaactgata cgcagaaaaa acgaaaatta
    17761 ccgcttcctt ggtatgtatt agggtttgtg atcttttgtt tggtcaacag cttgttttct
    17821 ttacctgtag ttattgacca atcggcacat tttatcagca gctggtttga gatcactgct
    17881 ttagcggcaa ttggtctgcg tttagacttt gcaaaattca tgaaagaagg tacacgcttc
    17941 ttatcttatg gattagctgt cgggcttctc caaacagtga tggctttact atttatttgg
    18001 ctgatcaaag tttagaatga acatgaaaaa acagttgtga ttctcgcatg gaacaatgtc
    18061 cgaatcacaa ctgtttttaa ttttgcagat atccttctaa caacaacaat tggcgtttga
    18121 tttcaacccc atctattgcg tttcccatat atcctgtcag ttttccattt tgcccgatca
    18181 ctcgatgaca aggaataacg attggcaatg gattggttcg atttgcctgc ccaatcgctc
    18241 gcacagcttt cggactatca acagcaacgg ctacttcttt atacgttcgg atttctccat
    18301 aaggaatggt cgacaaagct ttccagacat tttgctgaaa aggcgtacct ctctttatag
    18361 ataaaggaac atcaaattct tttctttttc ccgcaaaata ttcttcaaac tgttcttgtg
    18421 tgcgcgttaa aattcgctga ttcgctgatt cgctttctga aattggctga aaagatacag
    18481 cagtcaatcc ggattcatct gcatctaacc ataaaaagcc gataggtgtg tgtattttca
    18541 ttttgtttgc tcctagtcaa tagactggaa cttcctcctt tttctctatc caaaactact
    18601 tgccaactaa taaaaattgt tcttaaaagt ttgataaacc gaggaaacat ggtaaactaa
    18661 atacgttagt ttacaaaaag aatacctgtt cttttacgta aaatcaaatt ttaaaggtgt
    18721 tatttaaggt ttattaaatt ttgaatgtaa accatatata aacatacgat tgaaaggata
    18781 gaaacgatga aagtatcaat tgttgtccca tgttttaatg aagaaaaaac tgttcctctt
    18841 ttttttgatg aggttgaaaa attcaaaggt gcccatgagt tcgaatattt gtttattgac
    18901 gacggatcaa aagacggtac gttagcagca atcaagaagc tagcctcagc ttatccttat
    18961 gtccgctttg tctcattttc cagaaatttt gggaaagaag cggctctgta cgctggattg
    19021 caagcttcta caggagattt agtgaccgtg atggatgttg atctgcaaga tccgcccgaa
    19081 ctgcttcctg agatgatcaa ccggatcact acttctgata tcgattgtgt cggcacccgg
    19141 cgttcgactc gtgaaggcga gcctgcgata cgcagtttct ttgcgaaaaa attctaccag
    19201 ttgatcaata aaatcagcga tacagaaatg gtcgatggcg ctcgtgattt ccgcttgatg
    19261 actcgtcaaa tggtggatgc cgtcctagaa ttaacagaat acaaccgatt ttcaaaagga
    19321 ttatttagct gggtcggatt taaaacagaa tatatttctt atgaaaaccg cgaacgtgtg
    19381 gctggcgaaa catcttggtc cttctggaaa ttattcaact actcgattga tggtatcgtg
    19441 aacttctcgg atgcaccgtt aacgattgct tcatttatcg gcgctttgtc ttgtgtcggt
    19501 tctggtattg caattttgtt cattattttg cgggcattgt tgtttggtga tcctacatca
    19561 ggatggcctt ccatggtctc gatcttcctg ttcattggtg gaatccaatt gttatgtctt
    19621 ggaatcatcg ggaaatatat tgggaaaatc ttcttagaaa cgaaacagcg tcctgtttat
    19681 atcataagag aaacagaaaa agtgaagcca aatgaattga agcctcacca ataatatacg
    19741 tcaaatgaag taaacatttt tttaaatgaa aaaggactgt gacaaaagtc ttgtcacagt
    19801 ccttttttcc gaataaatgg tgttccagaa gtagcttctt cggaaataag ccgaaatttc
    19861 acaaaaattt gagaagcaat tttcgtaaat tccttcttat ttcttgaagc tgaccacttc
    19921 tatcacaacc tctttcatat cttattactt cttcattgct gcttttagtg ctgctgctaa
    19981 tgggctttct tctggttctt ctgcttgtgc atattttttc agcaagcgtc gttcttcatg
    20041 tttggtcatt ttcttcttct gctctttttt atcaagcaat ttttctgtcg ttccatcgta
    20101 tttacaacgg aaataagcac cgtttttacc ttcgatgatt tccattttcc ggtgacattg
    20161 cgggcatcga tgattagaga cttttggatc ttttcttctg cggtagttgc aatctgaatt
    20221 ggtgcagaca tagattttgc cgtcacgtgt gtttttctcg cgcagattgg ccccacattc
    20281 tgggcatttc ttttgggtaa tcgagaaatc ttgatattct tgtttgcttg atttgatctc
    20341 attcaccaag cgttttgttt ctttttcgat tccattcaag aaggttcctg cttgtttctt
    20401 tcctgcagca atagcttcaa ggtctttttc ccagctttct gtcaaatctg gtgttactaa
    20461 agagggattt accaatacta ataattgctt acctttagga gatacttgca atccttgagg
    20521 ggtacgttcc atcagttcag atttgattag tttctcgata atctctgcac gcgtagctgg
    20581 ggttcccaga ctgtattttt ccatttgacc aagcaagctt gcttctgaca atggtttagg
    20641 cggtgcagat agttcttttt tgatggcgaa atcgggattg attctcattc ctttttccca
    20701 tttgactgtt tccgtctcag tttcagtttc gcctttccat cctgcttgga cgacacgatt
    20761 ttgccgaaaa atgaatcgtt ctttaccaaa ttcaaccgta acttttgttt ggctgacttt
    20821 atggggttga gcaaacagtc ctaaaaaacg agagacaatc atttgataga tttttcgctc
    20881 ttcattactt aatttttcat aacgtgctct gttttcagtt ggcaaaagtg catggtgatc
    20941 ggtcacttta ctgtcttgga atacttttgt ctgttggacg atggctccgt ttttcaaata
    21001 ctgcttgacc tcaggagcaa agtcgctgac tgcttgcaat cgttctttca tcgtactttt
    21061 catgtcattt gtcagatatt tgctgtctgt acgcggataa gtaacgatct tatgggtctc
    21121 atacaaactt tgtacaagac ttaatgtttt ctttgcagaa tagccatagc gttgattggc
    21181 ttctctttgg atctctgtta aatcataagg caatggcgca gattccgttt ttaatttttc
    21241 ttggacttct gtgacagttc ctttgcttgt actgatcaat tgaacaagtt tttgtgcttc
    21301 ttcttgtgtt tttaaagcaa attgattttt ttgaagcatt ttggcttgtt cttttccagc
    21361 ttgcaacgtg atcgtgaaat acgtttgtgg acggaactgc tcgattttct tttcttggtc
    21421 tctcactaat gctagtgtcg gtgtctgcac acggcctgct gataaattat ccgcgtactt
    21481 cacagttaag gctctggtca cattcaatcc taccagccag tccgcttttg cccgggcaag
    21541 agcagattca tacaatgcat catattcttt cgcaggtctt agttgcttga atccagtttt
    21601 gattgcttta tctgtttgag aagagatcca taaccgcttc actggtttat tgaaatgcac
    21661 atactctaag atccagcgag caacaagttc cccttctctt ccggcatctg tcgcaatgac
    21721 tgcttctgag acatcttttc gctgagccag ctgcttgatt gcttttaatt gatgtcctgt
    21781 tttaggaagt ggtttgattc ctagtttttt agggatcatt ggcaacgtgt ccatttgcca
    21841 gctctgccat tccttattca gatcttccgg cattttcaat cctaataaat gaccaagggc
    21901 ccaagtaacg atcaccttgg gtccttcata ataatttttg tgcttttgat tagcaccaag
    21961 caccttgctt aggtctttcg caacgcttgg tttttccgct agaattagtt gtttcataaa
    22021 attcctttct gctttctact gtgatgggct gatgattttt caacaacgca agtcccttgt
    22081 attgctgtaa atcatcgtca cactcttcac accagcgctc atctccgtca ttccaaaagg
    22141 cgctgagaaa gttacttttt gcattgatat attcataatt ttgatggatt tcgttccatt
    22201 gtttttcgta cgcttgtcgt gcttcttcga aagactcgaa tgtttcttct tcttcaatat
    22261 cttcttccca gtcttcaaaa aaccaccaag gctcattgtc cccgaacatg gtaattactt
    22321 gatacatact cgtgctcctt ctttcagctt ttctagactc atcttagtca cttttcaaga
    22381 aagttgcaag aattttctct tataaaaggg tcatttaatg aacatcttaa aattgttaca
    22441 aatgaatctg attgtttaag gcaaagtagc gataaagtta ttgatcatcc aaaaatagct
    22501 gtcttctgga tcgattgttg gttcttttgt aaagtatcct aaaaaacgta aggaaagata
    22561 accgtggatc atactacgaa gttccctgct tcgatgaact ttttcttctt tcgttaattg
    22621 aaaagcctca aatatctgca aaatgatctg attcgtttca tggattccat ctaataaaac
    22681 agcttcattg gtatgcggta cactgatcag taactcatac actgcttgat ttgaaaaagc
    22741 aaaggatcga taagtttctg catagatctg cagtgcttcc tgaccacttt ttcccacaag
    22801 agcccgtcgc agctggtcat taagttcctg cagaagtttc gctgttagcg ctgttttgac
    22861 atcacgaata tttttgaaat ggttatacag cgagggagcc ttgatatcca agcgtttggc
    22921 taaatgctga aaggttattt gctgaacatc catctcttct gctagttctc gaaagcagtc
    22981 gatgattttc tcttgagaca gatttctttt tttcatttcc atgtttcctc tcataaaata
    23041 aatccttcac tctttcagtt taccacgaaa aagaaagaga aaaaagagac tttcattgat
    23101 aaactacata ttgtagttta taataaagag ctgtagatga aaattttcag aaagaaggag
    23161 tctaattggc tgcaagaata aaggaaaaat tatttaggaa aagagaaact tgggaacaaa
    23221 acttgtttgt cttatggttt ggaacattta tggctggtat cggatttagt ttagtcatgc
    23281 cttttatgtc actttacata gatacgttag gtaattatag tacgtcacaa ttgaactttt
    23341 ggagcggatt gactttttca tctactttct tggtgactac gcttatctct ccttggtggg
    23401 gaaaattagc cgaccaaaaa ggaagaaaat tgatgctgtt gcgtgcttca ttaggaatgg
    23461 caatcgtcat ttctttgatg ggttgcgtga ctagtgtgta tcaattagtc gcattgcgtc
    23521 tgcttcaagg gattttttct ggttatatca gtaatgccac tgcccttgtc gcaactggga
    23581 cgccaaaaga aaaaagtggt caagttcttg gaaccttagc aactggttct gttacaggaa
    23641 cattacttgg accgcttctt ggcgggatct ctgcttcagc gtttggctat cgccctacct
    23701 ttttcattac agggatcatc ttatttttcg tcttcttgtt gagtctgttt ttcgtccatg
    23761 aaaaatttgt cccagtgaaa aaagaaaaca tggtttctgc caaacagatt ttcaaagact
    23821 tgaaatatcc acacgtcgtc atcggtatgt tcgtcacaac gatgatcatc caagcatcca
    23881 acaattccat tagtccaatc atcagtctgt acattcgcca attgctgcat ggacatggga
    23941 atgttacgtt gatcagcgga atcattgctt cgatacctgg gattgccact ttgatagccg
    24001 ctccaagatt tggtcgctta ggtgacaaga ttgggagtga aagaatactt gcgattggtt
    24061 tgggttttgc gattctggtt tatatcccaa tggcattcgt cacgaatgtt tggcaattag
    24121 ctgccttacg ttttttgatt ggtatctctg atgcttgtct gctccctgct gtccaagcat
    24181 tgatcacaaa atattcgcct catcaagctg ctggacggat cttcagttat aaccaatctt
    24241 tccaagcaac tggtaacgta gtagggccta tgatcggctc cagtgtttcc agtgcatttg
    24301 gttatcgtgg cgtctttctt tcgacttctc ttcttgtttt ggtcaacttc ttgcttgtac
    24361 gacacaatac acaagagatc catcagcaag aaactgcaga agaaaaagct tcaggagtct
    24421 ccatagcttc agggcactct ccttctcata gacaataagc gtaaatgaaa aacactgctt
    24481 gaacagttgt cacagactgt ccttgcagtg tttttgttta ggcctgtttt ttcaagaact
    24541 cagataagag gaataatccc ttttccaagg tttctttttt acaacagtaa cctagtcggg
    24601 catgcttcgg taaatcaaag gcacttcctg gtactaatag tactcctgtt tcttctaata
    24661 aattgataca aaatgtctgg tcatcttctt cgatggctaa tttaatgaaa gaagtagaga
    24721 cgtaattggg agcgacaagt tcaactttcg gttcattggc tacccattgt tttaaaatca
    24781 caaggttctc tgtaatgatc tttttgtttc gcgccaatat tttttcttta ttcttcaatg
    24841 catgtacagc aagatcatct gacaggactc cgccgcaaat catcgtgtag tctcgatact
    24901 ttcgaaaaac ttctgccaac tcaggtccag tagctgtcca gccaatacga atacctggaa
    24961 tggagtaggt tttggatagg gaattggttg ctataccttt ttcatagaca tctacgatcg
    25021 acaagaattc acctttatcc gttaaaggcg catatacttc atctatcaat acatatgcat
    25081 ctactgtacg agcaatttct gcgatttttt ctaaagtttt ccgatctagc aatgtgcctg
    25141 taggattgtt tgcactattc aaacagatga tttttgtttc aggtttgact aaggcttcta
    25201 gctgttctaa atcaaactgc caatcttctt cttccttcaa atgaacaaag tcaacagcgg
    25261 cacctaaaga tttggggatg tcatataatt gctgataagt aggaagcatc gaaacaacat
    25321 ggtctcctgc ttccactaac gcgtatagag ctaaaagatt agcaccagtc gctccattcg
    25381 tttgtaagat gttttccgga tcgacggttt gataaagaga agcgacttct tgtttgaaaa
    25441 catctgatcc ttcgatccag ccatagtcca atggttcgtt tttgcgtttt tcgaaaaatt
    25501 tacttacgct agtcccatct aagccaacga gttcgtctaa tgtcaatgct tcgatcgagc
    25561 tttgtgcaat atcatatacc gctttctttt cccatttatt tagccattct tctacaccga
    25621 aatttgcgat tttcatgttt ctttccccct tatatcttga tccagcctac agcaagcaac
    25681 ccgattgaaa gcagtgcggc cacacaaatc agcccaatca ttactttttc ttttcccgta
    25741 atttgatgtt tggtttcacg acaagcgatc cagtagaagc aaaaaccagg aagataggca
    25801 atactacata aaaggacata agaaaatcct gctaaaatga tgcccatcaa ttcaaaaact
    25861 acagcaatcc caccgatcac tacttgtttt ttgattcctt cttttaaaga aaactggact
    25921 tgatagatcc cgacaagtag ataacaaatc aaaattgccg ctgtacataa agaataggca
    25981 aattgatagg cataatcagt aactaagaaa gtcaataaga atagattagt caagattgca
    26041 gtaacaacta acgaataagt tggggcattt tttttattcg tttttcccca acctttcggc
    26101 aacagaccgt ctcgagccat caacatcgtt gtttctgctg ggagcatcgt ccaagacaac
    26161 caagaaccaa tgatcgaaat aatcaagccg atattgatca gcgctgctcc ccaatttccc
    26221 acgattcctt ttaaaatatt tgccatagca ggttgtgaaa tagttgctaa ctcttcttga
    26281 gataagacgc cgtatggcaa gatcgaagca aggatataga taaacaataa acaaagcaga
    26341 ccaatgatcg atgcttgctg ggcttctgaa cgtttcttcg cgcggttggc taaaacactt
    26401 gcgccttcga tccctacaaa gacccacatc ataaccatca aacaaccttt aatctgcaac
    26461 cagatcgttc cttgctgccc tgttccattc actagattat cagatacgtt accccaaaaa
    26521 tcagcagtga atatattcac tttaaatgcg ataaatacaa atattaaaaa taggaataat
    26581 ggcactaatt tacagatcgt cacgattgta ttgatgacgc tcgctccttc aactccattg
    26641 tttactaata aagtcagacc ccaaataaaa atacttgcaa ataaaataga aggaacattt
    26701 tgtccacctt tgaatatcgg gaaaaattct cccatggcgc tcatcagcat cgttgcaaaa
    26761 gcaacatttc ccagccacgc agacaaccag taaccccaac cactgatgaa gccacctaat
    26821 ttaccaaatg aagcttccgc gtaaccgaaa ataccaccat ctagatctgg acgcttttct
    26881 cctaaatgat tcagcgataa taccaaagct aaaataccaa tcccaacgat tatccatgaa
    26941 aggatcgctg gccctggagc tgcagaacta gccagatcac ttgtgatccc aaacacaccg
    27001 cttccgattg acgaactgac aaccagagca accagtcccc atttgctaat acccgtcgat
    27061 ttcttttcca tttccatcac cctcacttat aaaattgaac aaatcacttg caaaagattc
    27121 ccttctattt tgcaactgct cttgcttaac aaaaagcaac tttattcata ttattggata
    27181 ttatgaactt tttacacaag tatttcaagt ataaaatgaa taaaaattaa ttttattgca
    27241 aaaaaacagc tatgctatcc aaaagaaaag gtgtttcttt tgttagccat agctgttttg
    27301 attcatttaa tatcgcttaa acgtattgta aaacttttgt ttcttgatat gcgtggtcga
    27361 tcaaaccacc acccagacat tccattccat cataaaatac aactgcttgt ccaggagtga
    27421 ttgcacgtac aggttcgtcg aatacgactt ctgcacgatt atcatccaat aagcgaactg
    27481 tcacaggaac gtcttgctga cgataacgga atttcgctgt acacttgaat tcttttggca
    27541 ttggttcatt cgtcgtaaag tggatttcac ttgcatccaa gcttgtggca tacaatgcag
    27601 gatgatggaa cccttgcccc acatataatg tgtttgtcgc tagatctttt cctacaacaa
    27661 accagggttc ttgcgaatct cctccgccac cgatccctag tccttgacgt tgaccaatcg
    27721 tataatacat caagccagca tgttgtcctt tgacttcacc atcaagagtg accatatttc
    27781 ctttttttgc tggcagatag ttgctcaaaa attgtttgaa gttcttttca ccaataaagc
    27841 agattcccgt tgaatctttt ttcttagcag tcgcaaggcc tgcgcgttca gcaattgccc
    27901 gtacttctga tttttccatg ccgcctagag ggaacatcgt ttttgaaagc tgttcttgtg
    27961 ataattggct aaggaagtaa gtttgatctt tattattgtc tactccacga agcatgtgga
    28021 ccacgccgtt ttcatcgcga gtgacttgcg cataatgtcc agtagctaca taatcggcgc
    28081 caagatccat tgcataatcc aagaatgctt tgaatttgat ttctttgttg cacatcacgt
    28141 ctgggttagg tgtacgtcct gcgcggtatt cggctaggaa atattcaaat acacgatccc
    28201 aatattcttt ttcaaaattc acagagtaat aaggaatccc gatttgagca gctactttcg
    28261 ctacatcctt ataatcttcc gtggccgtgc atacaccgtt ttcgtccgtg tcatcccaat
    28321 ttttcatgaa tattccgatc acgtcgtatc cttgttcctt taacaaaagt gctgttacag
    28381 atgagtcgac accgccgctc atccctacga caacgcgtgt tttgctgttg tctgtcatga
    28441 tatcaccatc atttcaatta gattctgaaa catctacgag ttgaaggtcg caacttttca
    28501 gtattttaca ttatacgtca tatttctgaa aatttcatcc cttaattatc aagtcgtaaa
    28561 aatactgttc tactggacct attcagtaca caaacatgat agtcaaccaa aaaatcactc
    28621 atcttttgag cattcgatat aaaaaattgg catactcata aaaagggcca gcctttcggc
    28681 aataaggaat aattttataa attgtgaaaa actatttttc aaaattgctt cttatcgctt
    28741 aaagctgcac gtctcattta caaccttttc tttttagatg ttatgagttg ctcagataag
    28801 tgattttgtt tactctaaga aaaaagggag gcaaaagata catgaattac tttatcagtg
    28861 atatgcactt tttccatgaa cgcttattag gaaaaaacga ctttgctccg cggctctttg
    28921 caaatgtgga tgaaatgaac caacagttga tcactagctg gaatgcagta ataaaagaaa
    28981 ccgaccatgt ttatcatcta ggcgaccttg ctatgcatcc gcaatatgaa aaagcaaatg
    29041 cagaaatttt ggaactggtc actcaactgc aaggaacaat ccacttgatc aaagggaacc
    29101 atgacagtcg cgcttttttc cgttatttag aaaaaaatga tcctaggctt actgacggaa
    29161 gtcccaaatt ttatttttat gatgtaggtg ccatcgttaa attcgaccat caacaatatt
    29221 acttgactca ttatccgatg ttgctcgggc ctaacgacac tatccgcaat cttcatggac
    29281 atatccatca ttacagcgta cctatcgaga acgatgtcaa tgtaggcgta gacgcaccgg
    29341 aaagagaact cttaaaggaa aaactaccat ttggcactcc gttatctgag caacaaatca
    29401 aagaaatcta tgaagcaaag aggaagtacg ataggtcgtg aaaaaactgc cctatatcaa
    29461 gtattaaaca aataaagata attactcata tttttcattt gagtaggctt attgccacag
    29521 gaacattagg atcagaaatg gagttgtaac aagacttctg tcacaactcc attccatact
    29581 attcgtcttt ttgctcatgg tgttctttta ccagtttttt ctgtaattct ttatcataat
    29641 ttcgggcagc tgacgttaaa aataaaacga ttccgcataa taaaatcccg attccggaaa
    29701 acaagaacat tttttctacc cctaaacgat cagctaatgg tcctgcaaat aataatccaa
    29761 ttggacctgc caggttcatc aaagagttga agttccctaa gacacgacct aaaacattcg
    29821 attcataact ctgctggatc attgccatca gcagtgtatt gaaatatggc gttgcacaac
    29881 ctgctcctgc attcaagata agaaagtaca agaacccttg actatcgttt ggaaccaatc
    29941 cgcttgcacc gatcgtcagg cccataatga tgtaggctat aataataggt ttcattcggt
    30001 ctttccatgt tcctataaaa ctgatcagtg caccgcctaa gagcattccg actgcgtaga
    30061 ctacttccac tgctcccgcc tgtccaacgg aaccaccaaa gtaatctagc gtcatcaatg
    30121 gatacagact gattgctggc atgaacaaca gcatcacaaa tgcaccattg atcgttatgt
    30181 accataaccc tttattatcc atcaattgct gcagcccaaa ttttgcatct ttaaaaatag
    30241 taagcggaac ttccaatatt tcaggagaaa cttttgggat cttcacaaaa atcaataagc
    30301 cgacaccaaa aactgccccc aatacatcta ataaaataag gtaattgaca ggaacgactg
    30361 aaaacaaagc cgcacctaga gctggcgcaa tgatgaaatt cgctgattgg accattccta
    30421 actgtccatt cgtccttgtt atgtgagaag atggaacgat tgtcggcata atcgactgga
    30481 tcgttggcat ttggaatgtt tgtgcgaccg aacgcataaa caaagacaca aaaactagcc
    30541 aaatcgggaa ggattctgaa atcgttccga ctactgctaa aataagcgcg aaaatcgcta
    30601 caataatatc cgtgacaatc aaaagagctt ttttatccca gcgatccact agcggtccta
    30661 caaatgggct tagtaaaacc atgggaatca ttcctagtaa agtagcaaaa ctcaaaattg
    30721 tcgccgaacc ggtttctctt gtcaaatacc agatgatcgc atattggacg accatactag
    30781 tgattcctga caaaaactgt cccgataaaa agaacaaata atttctcttc caattcgtta
    30841 aaacgaccga ctgttcattt tccataaaca tccctctttt ctttttcatt ttgtatagcg
    30901 tttgcacaac aatcattata cgggattctt ttttcaaaaa ataccttttt ccctaaataa
    30961 caatcgtttc aatttaaatt tctttactta gaaacaataa atcagcaaaa tattaaaaat
    31021 aacaacatgt gttaaattta aaaataaaaa agacaactaa tttgcgtcgt ccttacaaag
    31081 ttagtcatct aacaataggc cgtttcaaaa ctagttgtct ttttatatcc acagatcatt
    31141 cgactttttc gaataaccca cggctaatca tcccatccat cagaaaataa aatttatctt
    31201 ggctcatttt ctgttttgga tctttgaaaa tattgactgc tttcaatcca tttgctgtag
    31261 tgatcgacat tttcaatgtt tgaatatctt ttgatacagt gatttttaat ttgaatcctt
    31321 ctttgctttg cggcgtggca tctaatggtc ggatcaattg ataattgcct ccgtttgttt
    31381 ctgtaaatcc attatcgcgc aatgtatatc tttttgcatc aggatgcaaa cgatagctaa
    31441 ttggtgcgcc cagtaccgtt gctgttgttt caaatgccat gattttcacc tcgataaaat
    31501 tagtttctct ttattataaa agtttatcct gatttttact aggtttttca gttaagttcc
    31561 ctaaaaaatg aaggcgttct ctcagacatc tgttcatata agaatgactt cactagttat
    31621 tttttgacag cacgaactaa ttgatcaagg aaatactgga tgtcgtcttc ggtatttccc
    31681 aaaccaaagc tgatacggat ggattctttt agtatcggtg tatcttctcc atacattgct
    31741 tctaatacgt gtgaaggctc gatatttcct gctgtacaag ctgaaccagt agaaatagcg
    31801 accccattaa gatcaagctt catcagtagc aaatcatttg cttcgccaat taagcgaaga
    31861 ttcaacacat gagggagttt attcgttaaa tcaccattta cttgataagg gatttcgttt
    31921 gcttctaatg tatcaagaat caattgctgg aatcctttat atttgttgtt acgtgcttca
    31981 cgttcttcag gtgacagcag ttcaactgct tttgccatac cccagatccc tgctaaattt
    32041 tctgtacctg cacgccgctt ttcttcctgc tccccgccta aaagcaatgc agggatattt
    32101 actccgtctc gtttatataa gaaaccgacg ccttttggcc cattgatttt atgagcagag
    32161 atacttaata aatcgattcc taattcttct ggatggatcg tttggtttcc gtacgcttgt
    32221 accgcatctg tatggaaata agctggatga tccgctaaaa gttcaccgat ttctttgatt
    32281 ggcatcaaat ttccgatttc attgttcccg tacatcacag agaccaagat cgtatcttct
    32341 cgtaacgctt ttttgacctc ttcgatcgac agattccctt tttcatctac aggcagatat
    32401 gtaacgtcaa acccctttgt ctctaaatat ttcatggtat taagaacagc aggatgttcg
    32461 atagctgtag tgattagatg tctgccttct ttttgacgcg agaacgctgt ttctaagata
    32521 gctgtattat ccccttctgt tcccccactg ttaaagatga tctcgtgagg ttttgcttgc
    32581 agactgtctg caatgatttg tcttgctgtt tccaattttt catgcgcgtg gcgaccaaat
    32641 ccatgtatgc ttgaaggatt tccaaacgtg tcttgcatga ttgccgtcat cgcttcaatt
    32701 acttgtggat gcatgggggt agttgccgca tgatccagat aaatagtttt cacttcattc
    32761 atcccttcca aattattgct aggcacattc tagcataaaa tagcgaacaa accaaagaag
    32821 agctcatttt tgtctagagg acgtttcatg ggcatagaaa aaaggagctg tgacaaaagt
    32881 cttgtcgcag ctccttgaag ctgctcactt ctgtcacaac ttctttccct cttttttttg
    32941 tttattcgaa ttctttttct tccagacgga ataacggact catcggtgta ttttcatgga
    33001 tgcgtttgac cgcttcaccc attaattctg aacatgtaac gattgttaag ttttctggat
    33061 gacgattttc ttctgtgaaa acagaatcag tgatacaaat atctttgatt ggtgcattgt
    33121 ctaaaatact tttcgcatga tcggacaata atccatgtga cgcacaggca tatacttctt
    33181 ttgcgccgtt ttcttttaat acatttgctg ctcgtgcaag cgttgctcct gtgttcaaga
    33241 tatcatctac catgatacat gtctttcctt gaacatcacc gatcacataa ccaccctcag
    33301 ttgaagcatc atctgcatga tctacgatgg ctaatgtgct gttcaaatat tctgaaagac
    33361 tacgtgcacg ctgaacaccg ctgttttttg gcgaaacaac tacgatgtca tctcccgtca
    33421 gacctaattg acggtaataa tgagcaaaca acggcatcgt aaataaatta tctacaggaa
    33481 tatcaaagaa tccttgtact tgaaccgtgt gcagatctaa agtcagtact cgagttgccc
    33541 ctgcttcttc gatcaagtta gcgatcaatt tcgcagtgat cggttcatga ggtttcgccg
    33601 tgcggtcttg cctagcatac ccatagtaag gcaatacgac attgatcgtt ttcgcacttg
    33661 ctcgcttcat tgcatcgatc atgatcagta gttccatata gtaatcattg acaggtgcat
    33721 tcgttgattg aacgatatac acatgatccc ctcgaatact ttcttcgata ttgatggaaa
    33781 tctcgccatc actaaattgc ttcacgacgc ttttacctaa ttctgttccg aacactttcg
    33841 cgattttttc agctaatgga cggttgccat tcaaactaaa aatcttcaac gtgtcatcct
    33901 gatacttatt actcatgtag tcctccccaa aacttctcta ttcttcgata gtttaacata
    33961 tttttccaag attttctgtt aatttcagca aattcttaag gaagtgaaac tgttccgtta
    34021 ctccaactgg caggaatgac tttatgataa tcgccccagt tgtcgtgtac tttgtaataa
    34081 cgttctcctg tttcagtttc aaaataagcg tacgctgtca cccaatgatt gccatatgta
    34141 ctgccaagta ctttcaaaat accaatcatc acaggtttgc cttcacgtat acgttttgta
    34201 gctcgctgcc atgaacctag aagagttgct cgtgcacgct catgattgcc attcttcttt
    34261 aaaaaactag aaatccctgt actaacctga aaaggaatcg taggaagtcc tagtggttgg
    34321 atctggctac gcaatgcctt cgtcaaaacc aacgaatcag ccgtattttt cttgcgaatc
    34381 tcatttggaa gcatccaccc cttacgatag tcttgataat aggcaaggag aactgctgcc
    34441 gcgtaagtac cacaaagata gcctgagggg gtagcccatt tcttatacct caaaaaacgt
    34501 tcttctggca atcccaccca gcaatttgcg agttcctttc ctgacctttt ttcttcgatc
    34561 aaaacgggaa tatcttcttt tctgttgatc catctagata actcgattgg ctcttcattg
    34621 atcagcttcc attttttaca agaaagaccc tcttgataag tatattttgc tggagaatat
    34681 tctaccggat gtcctgattg atcgataatc acataacttt gatcttcaaa aaccagagct
    34741 aaaaaaagca atttactttt tcttgactgc agctgctgag tgctggctaa ttttttaatt
    34801 tttttatcta ctttgaaata taaaggttta tccactactt gattcagttg ggatcgcctc
    34861 ctttttttca ttataagtga aggattgaaa aaaacaaatc agtttgattg ttcgaatttt
    34921 caatttttta tgttctattt gttaattcct tcattttgta agataatggg cacacgaaac
    34981 aaaaaaggag cgatcgatga atgaacaaag aacgattgaa acaagatttc tatgaagcag
    35041 tcaatgaaga atggcaaaaa acagcaaaaa tccctgcaga taaaccagct acaggcggtt
    35101 tccaagattt agttgacggg atcgaccaac ttttgatggg agaaacagac gatctgcttg
    35161 cccatccaga aaaagtgtct tcagaaaaaa tgaaacattt ccttgcatat tatcaactgg
    35221 caaacgatta tgaccgaaga aatgagttgc atgctcaacc gcttcaacct ataatcgaaa
    35281 aagttgaatc attgcagtct ttcgacgaat tgaataagca atttccagaa tggctgttgg
    35341 atacattacc attgccattt aatctggatg tagatgcaga tatgaagaat gcgcaaacga
    35401 acgccttctt tgcttatccg ccttctttat tccttccaga taagacgtat tatgcatcag
    35461 accatcccaa cggtgcacaa ctaataggcg ttttctttga tatggctgtt cagttattag
    35521 agcttgccgg caaagaaaaa agtcaggcag aagcaatcac ggaacaagca gtgcaatttg
    35581 ataaattgat tgctcctcat gtaaaaagtg cagaagaaaa cgcagattac agcaaaatgt
    35641 ataatccgca atcttttgat acatttatta aatacacaga taaactggac ttgaaaaaac
    35701 ttgtcacggg tttgattggg acaactccag ataaagtgat cgtaactgat ccagtgtact
    35761 tcgatcattt gaatgacatc ctgacagaag ctcattttcc gttattcaaa agctggatga
    35821 tcattaaaac agtacgttct cttagtggtt atctatcaga agaattccgc caagtcagcg
    35881 gaatctattc tcgcgtattg tctggaacag acgaagcaat gcctcagaaa aaagcagcgt
    35941 attaccttgc ctctggacaa ttcgatcaag tagttggcga ttactacgga aaaaaatatt
    36001 ttggcgaagc tgctaaaaaa gacgtagaac aaatggtgaa aaaaatgatc gccgtttata
    36061 aaaaacgtct agaagaaaat gactggcttg gggatgcaac aaaagaaaaa gccgtcataa
    36121 aattaaataa cttaggtatc caagtaggct accccgataa gatcccagca ttattcgacc
    36181 agttcaaaac agtacctgca gatcagggtg gtactttgct ttcaaatact cttgttttca
    36241 gcaaactcac attaaaagat cgtttcagca aatggaacaa accagtggat cgttcagaat
    36301 gggaaatgag tgctgacacg gtcaatgctt attatcatcc atttcggaat gtgatcgtct
    36361 ttccggcagc gatccttcaa gcaccatttt acagtctaga acaatcaagc tctgctaatt
    36421 atggtggaat cggagctgtt atcgctcatg agatttccca tgcatttgat aataacggcg
    36481 ctttatttga cgaatatggc aacttgaaca actggtggac agaagaagat cttacacaat
    36541 ttcaaaacaa agcacaagca atgatcgaag aattcgatgg tctgcctttt gctgacggaa
    36601 aagtaaacgg aaaattaacc gtttcggaaa atattgctga tgcaggcggt ctaagctgtg
    36661 cattggaagc agcaaaaacg gaatcagatc tttctatcga ggaattcttt gtcaattggg
    36721 caacgatttg gcgtacaaaa gcaaaaaaag aataccaaca acttttactg caaatcgatg
    36781 tccatgcccc tgctaaactc cgagcaaacg tgcaaccaaa aaaccttgac gaattctatg
    36841 aaacatttgg tatcacggaa caagacgcta tgtatctagc gccagaaaaa agagtgcgaa
    36901 tctggtaaga ggttgtgcca gaagtggtca gcttcaagaa ataagaagga atttgggaaa
    36961 atcgcttctc aaatttttgt gacatttcgg cttatttttc gaagaagtta cttctggaat
    37021 accatgtatt agaggttgtg aaaaagctgc ttagtttcaa gtagtaagaa aaattttgtg
    37081 aaaatggctt tccacatttt tgcataaatt tgacttacta ccgagaaact ggcttttgaa
    37141 caccgtttat cgagaggttg tgaaatcagc gtcctgacct gagtattaga gaaaaaaacg
    37201 gaaaaatagt tcctcatatt ttgccgtttg ttgagctaat accgaggggc agctgattga
    37261 acaccgttta gtggagagat tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca
    37321 atcaaaagca gcaaggagaa cctcatctat aaaaagatag aaggaagtga gacatctatt
    37381 ggtctcattt ccttctattt ttactttcct cagtcaattg ttttacatca taaaaggaga
    37441 aattccactt atttttttcg ctgtttcatg acataaacaa ctacaccgat taacaaaatt
    37501 cctccagcta atgctagcaa cgggttatta aattctcccg tttttgggag tccttgagtg
    37561 ccattttttt cactttcttt tgctgtccta tccggatgag aaggttttcc atttggtttt
    37621 ccatttggtg attcatttcc tggattttct ctttctggtt tgtcatttcc cgggggcaca
    37681 tcggcagttt ctcgcacagt gatcgctttt tgctgggtaa cagattctgt atgagcagac
    37741 catttttccc catcaaatat ttccgaacga taagtgactt ctagcgtata ttctcctttt
    37801 gaaggtgctg aaaaaatagc ggtatgatta gaaaattgag cacttgactg tgtttcaacg
    37861 atttggatct tttctgggaa ataacgcata tcttcaaaga caggcgttcc ttgatttggt
    37921 gtgaaagttg acacaaaaac tgtaaaaggc ttcagttcaa tgatatttcc ttctgcattc
    37981 tccgccacac catttgcttc ttgtataata atcggattat tttgttcatt tggttttgtc
    38041 tgatttggtt cttgtacgat tgcatactct ggatattccg aagacggtga ggtttctccg
    38101 tcaaaaatag cacctacaga taatggaaga ccatccactc tttccttttc tgtttgacga
    38161 ttccccggtt taatctgcat cagtccatga acatggatac ttccattttc atagcgcgta
    38221 acaggctgct gaccttcttt cgaacatgta tttagagact cgatccaatc ttttgatatg
    38281 gtatgtccaa gagtattttc tgattgtttc tctcgttcat tccaatagta gttcaacgag
    38341 tggtagattt gatcttcgtt ttgattcaat gggcggatct gctctctcat ctcaagattc
    38401 tctgttcgga aagataagat accagaagca tggtagtctg tgtttcctgc tgaagaggta
    38461 taaagagcca cattatatga accgttatta aaacttgtac tgctattgac gataatatct
    38521 gttccgctat tggaatcgat tcctttcgca agattttcga aagacaaact attgatcaat
    38581 tcatgagggc ccgttaaaga agaaccgccc attttgaatc cattaccgtt accttctccc
    38641 tcaattcctg gtacccagcc gtttcgataa gcaactgagt ttttgatgat tacttttccg
    38701 atcgaacctg tttcattttt agcaaaaagg tcccaaccgt catctgcatt atgatacgcg
    38761 atacaaccgt caaatacatt tccttctcct attgtcagtt tggccgcaaa tccatcggca
    38821 tcttcaaatc ctggatcggc attttcaaaa gatgtacagt tcttgactaa gttatgcgcg
    38881 ggccatgctt cgaaaggatc atttgagcta ccgctgattt ggattcctgt atttccattc
    38941 cgataagcat gaatctcttc gatcaaatta tgatttccag aaatctgtaa tcctttttgc
    39001 atagctgcac tatttgtaat gtcaaaccct cgtaaacccc agtagtgact gaacagtgtc
    39061 atcccatttc cagttccttt aaaatcaagt acaggacgct tgttttctgg atttttctct
    39121 gcaatcaact gaatcgggtt ttctcttgtc ccgttaacgc cttttggaat cgtcaatcct
    39181 ctttcgaagg aataagttcc aggcgccatg acgattgtct gcccagcata tgcgtatctc
    39241 aatgcttgtg tcagagataa tggattttgt ctgcttccgt ttcctgcatc tgacccttct
    39301 ggtgttacat aaactgtcga atatttaggt ttacggtagt ccactgtatg aagaatctct
    39361 tttgtttcat aactagtcat ctctacgtat tcttctggcg gatagttttt atctggcgta
    39421 aaactgaaag taaattcgtt cgttccttcc atcagttttg tctgataatc aaattcccga
    39481 ttcgcagtga tagccaagtt ttttgcgttc ttaagcacct ctcctgttgc tttattttta
    39541 attgtcaata atccgtcagc atttgctcta aaggacatag tatgatcttg gtttcctgaa
    39601 tgattactag aggtcacagt gtaaatcgga tcaatcaagc gagtaggtct ttcttgccca
    39661 ggaggatctg tcgcaggatc tgagtaagca acttcgattc cttctacctt gatcgtcata
    39721 ttccttgctg cagcaaatcc tgcataaata accgattcat ctgaagcgta taatttttcc
    39781 cagtcataca tgatgtcaga agccatttct tctcctgttt caggatcgta ataatatgcc
    39841 tctaaaccag tattcgtttt tttcatctct acgtccagtt tagtaatcga gttcgtggcc
    39901 ggaaacagat tcccatgacc gtttccgaat atattataag aacctgtttc tttttccaag
    39961 aaccctgctg aagtctctaa aggtgttgtt tccactgaga cactcccagg agctggcgca
    40021 gcttgcggat cttcagatga tattcctgtg attgcccgtg tccccaatcc aagtcgcatc
    40081 gtatatttgc tgccgctcgt gttgaccact tcttgtctat ccgagtccca gacatattcg
    40141 atccgactgc ctagtatggc aaatgaattt gaataaaatg cctgcccaaa caaatgattt
    40201 tttggtactg cgtctcttac cattaaagca aatccttctt gggcattcgt ataggtccaa
    40261 gattctattt cgatcgtcgc ccgcatccta aagttgatgt ctttggggat tgcttgataa
    40321 taataggtca ttccatctga tccgcttgac tggaattttc cgccgttatt cgtggaagag
    40381 agccgcacgc cgtcttctaa tggttcaatg gcatacctac tagcacttgt gctttctccg
    40441 aaaacaaccc ctttccactc ttcatttgct tcttcagatt gcttttttgt cttttcattt
    40501 tctgattctt tcatcatagg atcatttcct tcttccgtat ccatatcttc actagttctt
    40561 ggtatttcct ccattgttga catcccttca atgttcacgt gtgcattttc ttcgttcaca
    40621 gactgttgtt cgttagcagc aacttcaacg acagaaatta aaaccggggt actgagcata
    40681 aggagattca tcattagcgc actgtatttt atacgttttt ttaacaagtg tattcctccc
    40741 gtaacaaatt agaaagcgtt tacttaaagt attacctaat ttatttttac tgtcaataaa
    40801 aaacaaaatt ataataagtg tattttataa aaagaaaaaa tataacttaa gttgttgata
    40861 ataaagagat ttattaacgt aaaaagaata agttttttta aaataacgta taaaaaagag
    40921 cataaaacaa agtcgtttgt tttatgctct tcttttatta ttttgtgctt tttattttat
    40981 caaatcactt tgttttttta taaaatttct gcgcctagtg tggactcaaa atgtctcaat
    41041 gcccattcat gtccaacggg atcgaaactt gccacggcat ctttgaaaat atggagtttg
    41101 tatcctagat tatacgcatc gactgctgta tgcaacacac agatatctgt acatacgccg
    41161 gtcaaataaa tatctgtgat ctggcgttct cgcaaacgga tgtctagatc tgttccacta
    41221 aaagcggaat aatgccgttt gtctatccaa taaacatttt cctcttgctc atagtcttgg
    41281 tataaacttt ttaaagagcc gtacaattcc cgtccacccg ttcctatcac attatgcgga
    41341 ggaaataagc ggttctccgg atgaaaggaa tcttttggat catgcgcatc aatggcaaaa
    41401 acgacaaaat ccttttgatc aataaacttc tttgtatgag cagtcagctt tttttcaatt
    41461 acttgtcctg ctgtcccagt agtcagcttt ccatcctctg caacaaaatc atatgtgtag
    41521 tcaatcgaga tcaatgcgtt catcttagtc ctccttatac gagatgtttg acaaattcga
    41581 tcaccatttc tgctgatcgt ttgcctgcgt cttcgataaa ttcgtcaaag ctttgtgtcg
    41641 ccgagtgatc tgctgtgtcg ctcattgcac gtacgatcaa aaatgggata ttgaactgac
    41701 gagctgtttg tccaacagcg gctccttcca tttcgcaagc taatgcttca gggaaattcg
    41761 ttaagatttc tttgatttta tctggcgagt cgacaaatgt atcacctgta acgatcaatc
    41821 cttcttttgc gttcaaattc gttgcttttg ctgctttaac catttcactg cgcaaatatg
    41881 tgcttgcttc ataatataga ggcattcctg gtaattgtcc tgggttgtag ccaaatcctg
    41941 ttgcatctgc atcaaaataa gctactttgt ctgatataac gatgtcacct acttgtaagc
    42001 cttcaccgat tccgccagct gaacctgtat tgatcaccat gttcacgccg tattgttgga
    42061 tcaacagact tgtggtaata gatgctaaga cttttcctat accggaacgt acgacgatca
    42121 cttcgtggtt acctaatgaa cctgaaataa ataaagcgcc tgcgcgttcc catgataatg
    42181 gttcgcttag gttctctctc aaaattttta cttcttcttc cattgcgccg ataataccga
    42241 ttttcatctt tttaattcct tctttctata taaatgcgat tgctaaaaaa accacacata
    42301 acattaggat aacaataata agagatttca tcaaaaaaga gttccatttg gtcgtctttt
    42361 cccgttcgct gatccttgtt ttcgtgattg gtttattctt tttgctttct ttacgataaa
    42421 agctggcaat ctttttttct tcttgctgat aagctgtttc tgcttttcgc tgttttttta
    42481 atgactcgct tgcttgtgct tgttgtcgct tgcgaagttc gcttcgcgta ataagcggtc
    42541 ctttggccat aagccgatcc ctctactttc ctttggtcaa tagttcccag tattgaatcg
    42601 cataaatcgt ctttgcatca caaatcaact gatctttcat cgcttgttgc gcctcttcaa
    42661 gagttagatg atacaactcc agtacttcgt cttcatcttg tgctaaagga ttctcgacct
    42721 tctctacccc ttgtgcatga taaatatgaa ggacttcatt tgcaaaacct ggtgaaagat
    42781 acatcgaagt aagatggctc agcgacttcg cacgatagcc agtttcttct tctaattctc
    42841 tagctgctgt catttcggga ttttgtcctt ctcctggatc gatttttcca gctggaatct
    42901 ccaaaatcac tttttctaaa ggttttcgaa attgtttgac aagaagcaga cgatcttgtt
    42961 cgtcaaaagc aatgatcccg acaccgccag gatgaaatac cagttctctc ttcgaagttc
    43021 ctccatctgg caaacgaact tcgtctacag caacatcaat aatttttccg ctatagattt
    43081 ctttgcgact gattgttttt tcttcaaatt gctcattgtt cagcatatct tctcctgctt
    43141 tctttgtaat cataactgct tttttttcat ttttcaattt ttctcaaaaa ctccttatta
    43201 gtttaccata gataaacctt agattttctc taaatataga aaatccttct ttagtccgat
    43261 actctttcta gtagtccgta tgctaaaata agtatgtaat cgaataacga aaagaattca
    43321 atactcaacg ataaatgaca tggagggttt attatgataa aacgaaattg gccgtggctt
    43381 ctagcattgg tcttgctatt tctctttgtc cgaaacaata gtttttcttt tgttctagga
    43441 cttttattga taggtgtagt tttagttttg tggggactct ttggttcgcg caggaaaaag
    43501 ggaaaacaag aagaaattcc tgcactgact gacgaacttg aaactcacta tgaaaaaagc
    43561 ggtatgacag cgaatgaaat ttcatttttt cgacaaacaa tgaatcaaac aaaaaatgag
    43621 atcaatcaat tgcaaatgaa tatgcagcag acagccaaac ttaaagcaat cgatctccgc
    43681 catgatccag tcaaagcagc gaaagcttta ttcaaagaat tggtgaaaga accaaaccga
    43741 ctgcatgaag caagtcagtt tctttatacg catctgccga atctagttga tttgacaaat
    43801 aagtataatg agatcaatga ccatgagatc aaaaacaaac agacctatga aaaactagaa
    43861 gaaagcgcgc agatcatcga ccagctgtca gctttaatcg cacaagatta tcaaaaattc
    43921 gttgctgagg atctagatga tatcgacgta gagatatctg ttgcgaaaca gagcttaaag
    43981 cgtgataata aatatgaacc agaacaaaag gattaactct tttgttcata aaagaagact
    44041 cggaacattc agatgatgtc tggctgtttc gagtactttt tctaaagtag agacattcat
    44101 gtaagaatcg agaacagatt caacaaggag gaatttttta tggatcataa accgactgaa
    44161 acaaaagatg ttacaccagt taacgataca cttgaagatt tattgaacaa cccgttttca
    44221 actccaacag atacgctgac cacttcacaa caagcggaga ttgatcagct ccaagagcaa
    44281 cagacagctg ctcgtttaat tgacaaacta ccggcggaac gccaagaaca agctaaacag
    44341 ctagcagcaa aaatcgatgc taatgatgca cagtctgtca tcagctacgg atcggctgcc
    44401 caagcaaaat taagtgaatt ctcacaatcg atgttgaatc atgtgcaagc acaagatatt
    44461 ggtcctgtag gagattcctt aactgaactg atgtatcgtc tccaagaagc caatcctgac
    44521 gaactgcgtg ccggagaagg gaacatcttc cagcgtgtct tcgggaaggt caaacaatcg
    44581 atctatgaag tgacagcaaa atatcaaaag attggtgcac aaattgacaa gatttcagtg
    44641 aaattagata aagaaaaaga cggtctgcta aaagataatc tgatgttaga acagctttac
    44701 cagaaaaaca aagactactt tgatgcactg aatatttata tcgctgctgg agaattgaaa
    44761 atggaagaac ttcaaacaac gatcattcca gaagcgatga aaagagcaga agaaactggc
    44821 gatcagatgg atgttcagat tgccaatgat tacacgcaat tcctagatcg tctggataaa
    44881 cgtacgcatg atcttcgctt agctagacag atcacgatcc agcaagcacc acaaatccgt
    44941 ttgatccaaa acacaaatca ggccttggct gaaaagatcc aagcatctat cgctacagcc
    45001 attccgctgt ggaaaaacca agtcgtgatc gcattgacct tgcttcgtca aaaagatgca
    45061 gtaactgctc aacgccaagt ctctgaaaca acaaacgact tattgaagaa aaattcagaa
    45121 atgctgaaaa tctcagccat cgaaacagcg aaagaaaatg aacgtggaat cgtggatatc
    45181 gagacccttc aaaccactca aaatgatttg atcgaaacaa tccaagagac attgcgtatc
    45241 caaaaagaag gaaaagaaaa acgtcgtcat gcagaagtag agcttggaca catggaagaa
    45301 gatctgaaac aaaaactgtt ggatttaacc caatagatca cctttcgctt gttactaaaa
    45361 caaacgccca acaacagttt attacaactg ttgatgggcg tttttcttat ctttcttcta
    45421 acttctccat tggagtctat ttctcattca tcttgctgtg tccatagcca tagccaaaat
    45481 aaatgatcag tccaataacc attacgatac cgaacacgat ccatgtcaca gtttgcagac
    45541 gaagcatcaa tacaatacaa gtcaagatgg agacgattgg caatagcgga acgaatggca
    45601 ctttgaattc tccagctttt ggtttgccaa acatttttct caacttcagt aagcctagac
    45661 taacaagcat caaataggcg agcgccatga tattcgttag ttccgctaat acattcaatg
    45721 ggaaaaatcc ggcaaagaat acagctgcca caccagcggc ataagtcgct tttttcggtg
    45781 tacgatgctt ttgatcgatt tctgtcataa acttaggaag taagccatcc ttgctgacag
    45841 caaacagtaa acgagcaagt gaatacatca tcgagatcgt taccgtcagt agcgttaaaa
    45901 ttgcccctac agcgatcacc gcgcccactg ttccatgtcc tacatacctc attgcaaaag
    45961 ccactgggtc attgactcct agttcagtat acggaacgat tcctgtcaag atcagcgtca
    46021 cgatgatgta aaggacagta gcaatcagta tcgaaccaat gattcctctt ggcacatctt
    46081 tttgcgggtt tttgacttcc tcggctgcca ttgcaacggc atcaaatccc aagaaagcaa
    46141 aaaacacaag agctgcacca tctagcacac cggaaaaacc aaaaggcatg aatggctgcc
    46201 agttatcagg tttaacgaaa aatattccta caatgataaa caacgcgatg ataccaaatt
    46261 tgacatatac catggcatta ttcaaacgta atgctttctt ggcttcaagc gacacgatgt
    46321 aagtgacgaa tatcactacg agcaccgcaa tcaagtcaat gtatgtacca ttttccggat
    46381 tgtacccgcc agttaatgct tgtggaagtt cggcccctaa acttttcaaa aaaccttgca
    46441 tatagccaga ccatccggaa gcaacagagg aaaccgctag taaaaattcc ccgatcaaca
    46501 accaccctgt cagccaggca gcaaattcac caaatacaac atacagatag gcatatggcc
    46561 caccaatcac tggcactcgc gaagcaaatt ctgcgaaact tagtccagac aaaatgacaa
    46621 cgattgcagc taagacaaat gaaagtgaca gtgctggacc agcattttga ttggccgcaa
    46681 cccctgtcac cacaaatatt cccgtaccga tgatcgcacc aattccaagc atgatcaaat
    46741 cagacgtttt tagttctttt ttcatctcac tcgtatgatt gagattgatc tctttcttac
    46801 gaaacaaatc cattgaagtt cctccccatt ttaggttgga aaacagcgat taaactgttt
    46861 tcatataccc taaaaggata ccacctaaaa tgactaaaag gcaaccaaca ataacataaa
    46921 tgagctcttt tttcgttttt ctttcgccaa ggatgaaaat cccacctaaa gtagagataa
    46981 tgatccccat ttgtgaaagt gagaagccaa cagctaaacc aagtgatttg actgttagaa
    47041 gcatgcagat gtttcctaag ccccataata atccacagat catgtttttc cacacaaact
    47101 gatcgacttt aactttacgg aaagcaaaga agcttgcgcc taagatcatt ccgatacttt
    47161 gaggtaagat aattgacatc gcatccaaat tcgcccagtt gacgatgatg gtatatacac
    47221 cataaccaat cgtagagaag atcaatgctc taaaaccctt accaaaatca agcattttat
    47281 tatctgtatc tatttttcct tcgtcatctt gtcgggcagt caaatacgca ccgcttacaa
    47341 gaagagctaa agcaataata ccgtatacat aatctttcgt ttgtgtccat tcatggaaga
    47401 aaacggctcc cgcaatcgta ttcaaaatta attgaaaacc agtagacaat ggtagcccta
    47461 cagaaacacc catgtacttc attccatgga actgtccatt ttgtccaaca ctccaagcta
    47521 agccggataa aaatccgatc aagaaaatcc aaggggtgat tacaggacta acaacgaaaa
    47581 agacaactaa agaaaacaga aatgcaccga tcgtcattcc tagtgtttgt tgattggcat
    47641 cgccaccaat tttaccactt actaaaccaa tactacccca tgccaacata gggactaatg
    47701 ctattaaaat ttccataacc aatctccttt tatttcacat gataagaaac ttatcagcga
    47761 aaacgttaac aacacaagca ctttttttcg tttctaacga ttttctaacg tttttcatta
    47821 taaaaattta agattgggaa aaataaagtc attgttgtcc cttttccttc agttgattca
    47881 gccgtcaaat ggatacctaa tttttcggcc agattttttg ctaaataaag tcctaatcct
    47941 gttgaatgtg ttttgctaag tcgaccattt ctcccagtaa atcccttatc aaagatccgt
    48001 ttcaagtctt ccgctgggat accgatccct gaatctttaa ctgaaaggct gactccttgc
    48061 gcttcctttt ctatgctcac aatgatgttt ccgtgatccg gcgtatattt gatggcatta
    48121 ctaagcaatt ggttgaagat aaaagcgatc catttagcat cggtcaaaac agtatgatcc
    48181 tcaccaacga tctcgtagcg aatatttttt tggataaaat aatttgcctg actgcgtatc
    48241 acaggctgaa tgattccttt tagtccatat tcttggatga ggtagtcccg cgagaaactg
    48301 tcgagtcttg cataatacaa aacttgttct acgtattcat cgatttttga taattcattt
    48361 tctagtaatg tatacttttg atcgtcaata tcgaactcga tcgaacgaac tagcaattta
    48421 gaagcggaaa gtggcacttt gatttcatgg acccatgagt caatataatc tttttggtct
    48481 tgttgattac tgattgctga ttgcattact tcttggtgtt cacggatcag ctgattgata
    48541 tattcttcta ccagtttttc ttcttctgtc ctcccaccac tcaaataatt ttgcaaaggg
    48601 gaagtttttt gtatgtcggc taattttccc caccatcttc tcttgctcaa gtaaccaccc
    48661 agcagataaa aaatcagaaa gacgccctca atcaaagcta aataagcaat tgtgctcaaa
    48721 tcaactttga tgtttggcgc cagccacatc acaaagatcg tcaatgcaat aaaaaacagc
    48781 catcccaaga tcaatgtcca ctgatctttc aaatatttaa aaaaattcat cttaaccgct
    48841 catcctttca gggaacgata tatccctgtc ctacttttgt ttcgatatat ccttggatac
    48901 ctgcctgttc gatttttcgg cgtaaccgat tgatgttgac tgtcaatgta ttgtcatcta
    48961 caaatcgttc gtcatcccac agtgctcgca gcaatttttc ccgacttaag atctttccat
    49021 tgtgcttcat caaaataaat aatagacgat attcattttt gctcaaatca atgatctctc
    49081 cgttgatttc catacttccg ttgtcaacat tcaacgtaat gccattgtgg ctcatcattt
    49141 cactagataa ttctgtgtag ttgtaagaac ggcgaagtaa tgcattgatt tttgcaatca
    49201 gtacatcgat ttggaaatgt tttgtcacaa aatcatctgc ccccatgttc atagccatga
    49261 tcatatccat attcgtattc ctgctggaga taaaaatgat cggtactttc gatacttcac
    49321 gtattttttg acaccaataa tagccatcca aaacgggaag attaatatct agtaatacaa
    49381 gctgtggttc ttctctttca aagtctgaga acacttgatt gaaatcggtc gttccaaagg
    49441 tttcaaattg ccatttctca agttcctcca tgatcaattg tcgaatgatc tcttcgtctt
    49501 cgactaccat gatttttgcc ataaaaaatc cctccatctt tcttcttgct actacaatat
    49561 caaagaagcc cttgtgaaac aagttggctt acctggaaaa tgtcatttta gcaaacgtta
    49621 aaaaaaactg gaaatatgcg atttaatcgc gtttgtctct tccaagcagc ttaaatttat
    49681 tatgttattc ttaataattg gtctattatc tgttattttt ctttcgttgt ttttgtcttt
    49741 taaattttcg ataacaccaa taaccgaaaa gcgttagcac tagcaatata cctagaagga
    49801 gtataagtaa tcgataaaga tggtattttt ttgtctgttc tatctttgaa gagacttctt
    49861 ctgtaaaggg aactcgtctt cctgtcacta gtaatcggtg agtgttcacg gtgtaaggtg
    49921 tacaagtgat aagtgtgact agatcctgct tttctctaat tgctaatgta tttgtttttg
    49981 ttggaaggac aacaattttc tcaacgactt cataagcgag catccgacca tacacttcaa
    50041 gatagaatcg gtcatcttgc tccatttctt caagatccgt aaagagtttt ttctcagcaa
    50101 taccgctatg ggcggtaatc acggaatgtg tactctctcc tccagttgga aaagaggtgc
    50161 cttgcaggac tgtcgctcct ctatctaaca aaagatcatt cgtttcatca aataaaggca
    50221 agctaacatt gattttcgga atatagatgg ctccgatcat atgttgttcg taatattctt
    50281 ttcctggatt ttggacattt ctaaccgctt gctcaaaagg atctttgact tgtcccattc
    50341 ctgggattac tgatgtatga gcaagcgtct gatttttctt ttcatgcact gctaattgtt
    50401 gctcatgttt ttcttcttta tcgcgttcaa gctgcttttg ataattctcg atccttcttt
    50461 gatccaaata attgctgaga gcatctgcta aaaatggata tgtaaaaact gcagctccgg
    50521 caagaaacat cactgccata aataattgta ataaaagact gatttgggtt tgaggtctat
    50581 tacctatttt ctttttcatt ttcttcacct tcttatttaa aaaaactgca gcaaccttta
    50641 taaaaccgat tgctgcagct tatttttgtt gcttatactt cagacttcat tttagattta
    50701 cggtaccagc tgtacgctcc aatcatcaaa cttattccga ttagtaagaa tgccaagagc
    50761 ccgttcccac cagtagcagg aagtgacccg cgtttcatat tcggtactct ttctggccct
    50821 ggattttcta ctcctacgat ctgaatttct tcattatagc ttccataaga gatgataaat
    50881 tcagtaaagg ctgcctcacc tgttggaagt acataacctt ctggcgccat ggtctctttc
    50941 atttgatagg ttccataatc taacccttga acctcaaagt atccatttcc atctgcgaca
    51001 aattctgttg cttgtccgct tgttgcccac tcaattctat ctgctcctga ttcataaaca
    51061 gctaaaggcg actccccttt ataaaatatt gcgtattcag aacggtcttc attccacaat
    51121 tcgaatcttg catctttaag caattcactg cttgatgcat catatttttc aaactttctt
    51181 ccacctgttg tgactttctc tggcggctct actccttctt cataatcaaa ttcatcattt
    51241 cgtccgattt caataaccgc tgtgttgtta tggaaatcat ccgcttctag ttctgtggaa
    51301 accttcaaat tatacgtgat cgttaaatct cctccacgtg catctgctaa tctacctaaa
    51361 gtatctactt tggctactcc attttcttct gtcaaaatcg tcacgacaaa cccgttgctt
    51421 actacttcta ctatataatc ttgtcctttt gttaaaatcg tctcgccggc tcgcacttct
    51481 atttcctgat cataataagt tagaccaact gtaggcgtat ctgtgatttt gtaatttttg
    51541 ataacagcgc ttccgttttc aaggacagaa tcgatgaaat aaggaatagg tgctgtgatc
    51601 gtgtaagaag ccgtttttcc tctttctaga tcaatataag aaatcgtttc ttgttctcca
    51661 gcttcattaa taatcgttac ttgtctaagg tcgctttcct ctacattcat ctgtttatct
    51721 gcttctctga tttcgttttt cggatacaaa tgcacatctg tattgtatgt gtctttccat
    51781 gtagaaccat cccacacgcc atccaccata tcaggcatca tcactggcat cataacaacc
    51841 attggagacg cgattctgga aatatgagct ggcgaataag tttctaagaa aagatagact
    51901 ttatctctag aagaaggtgc ttcttccgtc acttggacgg tagagaatac cgcttctcca
    51961 gcttcattcg ttgtttgttt gtcaatactt gtcagtcgat attttaaaaa ccaactttct
    52021 gtgtgccgtt tttgaatcca ttcaatcgct tcggcagaat taagaccgtc ttctcgttcc
    52081 ggtgttaaag gattatctcg aatcaaatcg taatatactt ccgttacgtc aaacaaatca
    52141 aaatcgactc ctggaatatt ttcttcacca aaatccgtag aaaccaatcc gctattcggt
    52201 cgttcttccg gaacagatga gaaacctttt ttgtgtaatg taatcgtcac ttcacttggt
    52261 ttttgtgcga cttcttcggc tttgactagt tttccgttcc cgaaaatccc tattcccgca
    52321 gtaacgagta acagtacact cattcccgtg acaatcattt ttttattcat ttttttctcc
    52381 ccgttctttt tttattaata aaacagcacc taacaataaa agaattccta gtaaagaaat
    52441 caacgttctt gcttcattcg tttgcgggaa tacggaccat tttcctgatt gacctggatt
    52501 gcttggacca ctcggctctt tcggtttgtc cgttgtttct tttacggtct cgttataaac
    52561 acgtggtgtt gcggattcat aggcagagac tggaactttc ccggattcta attcatccat
    52621 ctcctgtccg ttaactacta catattgtgg ttggcctttt gcatccaccc aaaattcagg
    52681 gatgatgacc tcgattctct tacttgcttc atccatctcg taaccttcca caccttttaa
    52741 ttcttcaaaa taataggtgc cagaaggcaa gaaacgttgt cctgtcgtaa caaggccctc
    52801 tttatccgaa atgaatttgc ttacattttt gtcattcaaa ggatcattgt ctgctggttt
    52861 gatccattga tttttcaagt cattagcagg agccatatct aaataatatt tccccccttc
    52921 tatcatctga tatagagcaa aaacagcacc ttctaatggt tttccttttt cagttgttcc
    52981 tctttgacgt ccatacttga agaagtatgg atctcttaga taaccgacat ttttaggata
    53041 gatatgaatc tcttgcagtt cctcgcttga ttctgtcggg tgataaattg gcaaaatagc
    53101 agcaattggg atagctgtct gatcgatatc aacatccaat ccaacttctg gatcaatcga
    53161 ttcttcgaaa atcaaataaa ctgcatcccg atcttggttt ttcttaggtg ctttgatacg
    53221 tgcaatcccg tctcctaatc cgccaaccgt atctttccca gttgttaccg aagtccctat
    53281 ttggacaagc ctttcttctg tgattagctg acggatattg tttctgttcg tttcagcgat
    53341 ttgctggacg aattctttag gagtcatgtt ttcagcagct tttttttcat gataataatc
    53401 ggtcatgtcg taaatggcaa atgtcgctcc atttagtggc accgtctgtg aaatgatatc
    53461 tgctgtttcc cctgcttcct cagagatagc caagccatca ttttcatggt attcaaattg
    53521 atcgttataa tcgatatctc gaatcatccg tttatgcaac acgaattcca cgacatcttc
    53581 ccctgattct tctgccgaca tcgcctgtgg aagaaggact gctgcaagcg gtagtaaaac
    53641 aagtaattga agcatcaaac acaacagctt tttcattacc ttgctccctc ctttttagat
    53701 tgtctgacaa acaaaccata ggctgctcct gcaagcgcca tgaagaatgc agcaagcagg
    53761 atatagaata atcgtccgga tcctcctgta gcaggcaacg gattttcagg atcgttcccg
    53821 acactaagct gtaatctgat tcgatttgtc tccaaaagtt catattctaa ctcttgctgt
    53881 agatcaggcg tctctgcttc ttcgtttgga taatacgaaa tctcaacttt tagtgtaccc
    53941 gcttcttttc ctggaactac ccgatcattt tctatgtcag gttcttctcg ataactaatt
    54001 tctaaaataa agtaacctgg aagttttcta tagctgtcag gtgacttcac ttccttgatc
    54061 cgatagaccc cttccaacaa gtctgaagca ttgaataggg aagaatcgtt tggatctggt
    54121 gccaagcctc cctcgatgat ttcatactct cctgcactat tttgtttctc aattgaaaat
    54181 tctgcgcctt caagacgtcg gctattcata ctgtcaattt tcaaaagttc caaatcgatt
    54241 ggcgctagtt catcttctaa ggttacttgt agtacaccat cgatgatttc atagccttcc
    54301 ggcaagggag cagtttcgtc ttcgatgacc gtatcttcaa tgataaactt accttcttca
    54361 ttcagtgcaa actggaacac ttgatcagat agccgatacc catttggtgc gacagattcc
    54421 gagacctcat attcaccaat agacagatgc agaggttggt cgtaatttaa atcgacatac
    54481 ttttctactc ctacggcacg agaatagaag tatccgatcc caggcaaacg tttcgattct
    54541 tctggttcta acggattacc agtgtatgct cgatacagcg ggaaggaaga atcccccact
    54601 ttcctgatat cgaaacctgc accatctaat gtatggcctt catggtctaa ttttctaact
    54661 cggatctccc gataatcaaa cgtattgtaa gcagtgaatg ctacccataa acgatcctct
    54721 tcatctgttg tatacgtgag atattgccaa ttttcttcac ctgtttgctt gagttggata
    54781 gtccctatag cattatcgta aagtcgcaag atccatgtgt cattttcatc aggttctttg
    54841 aaaccttctg ggttctggct ctcaatgatt ttgtattctg catagttggt agtttcttca
    54901 ctatccttat aaacaaatga atctggctct ccgattggat gaataacttt ctcaccatcc
    54961 acgattacat atttcccgtc ttcatctact gaatagaacg tgagtctccc tttctcttcc
    55021 tctacttcgt tcacaccaga aagccctttc gctactggat atccgatttc cagtccgtta
    55081 ccatctgttt cataaagttc aaactctgcg ccattgattg gcatattact gccttcaatt
    55141 agtttattga tattcatgtg gattggcttg aattcatttc gcatttccag tttgacatac
    55201 aactcatcat actctgtgcc ttctatgtta ggctcttgta caacagaaag cttgtgatat
    55261 tctccttcat aataggaatt cggatcttta ccttccggtc cgccgactct cgaaccagat
    55321 gtatattcat ctacaagctc gtagtgacct aacatctcat cttcgttggc tgcatatttt
    55381 ttgatttgga attgatacgt ctcatatact aactgataac gtggtggtcc agcgatctct
    55441 ctgatccaaa atatgtcgct gactgctcca gtttcatctc ctaaaatcgg catcttcgtt
    55501 tcaaagatcg ctattccgtc cgtggtagtt ttacttgttt cgattggttc ggcttcccca
    55561 ctctctggat tttggtcata taatgcaaat tcgactccag ccaacggctc gccattttga
    55621 cccttcttgc tgacttggaa attacggcct ttatgttcgt aacgaatgga agaagatggg
    55681 aaatacatcc gtccagtagt gatatcattc tcataatcct ccaggttctg aatataggcc
    55741 atattgttca ctgtgtaaaa atttccgtct tgagctcccg catcaagttt tacttggtac
    55801 ttgatcctga ccatttcccc ttcacctaaa gaaatcggag aaaatttata agcttggttt
    55861 tcccgtccga catacgttgt cggagtcgcc actacttctg gtggggcttg tccatgacgt
    55921 tcttcatccc atatttctgg ctcttggtac ttcatgccgt tttctcctgg atctagatgg
    55981 taaaactgaa tatctgccga tctgccagct ccaaatggtg agacattaaa accagtttca
    56041 tcataaagtg tggcttgctg gatcgttctc ttgaaggtag aagagatatc ctccaaggct
    56101 gtaacaatgt cattcttgtt gttcgcattg taatatcgcg gtgttacgcc atcttcttct
    56161 aaatcagcaa tatttttcaa tacatttcgt ccaatagcat ctagtcgttg tctcccgcta
    56221 acgtttccat cgattcctaa accaatggag aagatacttg gcgggttctc ttgatacttg
    56281 tttttcagcc aatgatgata agcaactgtg ttgatcgcag atgacttttc ttgcgaagta
    56341 ggactgccat tcgtattgtt attagaagaa ccatcaccaa cccagtgagt cgctttatta
    56401 tctccttctt ttacctctcc ataacgatag tgtacattta cttcatcttc tggacggtta
    56461 aagtcagatg cataggtaac tggatgagta aaccctgcat tagaagtatt cccagaaaac
    56521 tcctcaaaat ttctgaacaa aacgtcatta tcttcagcaa tcttatcttc catcacagtc
    56581 cagttgctcc aagctccatt agtgctcgta cgataacttg actgaatcgg tgcataactg
    56641 aaggtcggtg tcccatctcc cacaacgatt aggatttttt ctggatttct attttctccg
    56701 ccattgtctt tatacaatgt ttcatagcca tttttaagcc ctagtgtaag cggagttcct
    56761 ccgattggtg tacgatttaa tgctgtattc tcacttcccg tcaaacgatt gatatcattt
    56821 gtcaatggaa agatgtccga actgatttgc tcatggttat tgatagctgt actattatgg
    56881 taaaaattca ccataccgat tcgaatatcc agactcgggt cagagagcag atctttactc
    56941 atttcgttta ctgcctcaat caacgcagca tttttagttt gactattggt cccagcggtt
    57001 aattcactca tactcgctga cttatctaga acaaaaacga tatcgattgg actgatttcc
    57061 tttttctcgc ccccgatgac atctagatag acattgtaaa gggttgttgc atcttcagga
    57121 tgtagatcag atgctgtgct gcttggcaga acaggttcta cccatttttt gatgtacgcc
    57181 ccgttataat ccaagtaact gttttcaaaa ttccgttcat ttccccaaag ctgaacggtt
    57241 tgagctggaa cgtttctatc ttcttcgcct gcagttgatc cattattagt agcatagtcg
    57301 taatttcgcc agttcgctcc cccgtttgta taatcactat catcgatcaa gttctctccg
    57361 tcatatggcg agcctttgtc tccttgatag cgttcattgc tttgtgtcca atttcctaac
    57421 acttttgtat agtcattcgt tgtaaatttc gggtagcgat gctgttcgtt ttcaacttct
    57481 ccgctaggat agatatattc ataggggtct tgcgtagctg ttggcccaag acgctccaaa
    57541 ataccgttga ataactggcg ttgaatgcgc tggagtgctt gaccgctggg tacgattggt
    57601 tcgtccagct tttccatctc attttcttcg ctacgttgaa tttcagcgga atcttctgta
    57661 tcagtttcat tttcttgtaa ttcttggtta tctattcttt catcaaccac cacttgaaga
    57721 ggtagttccc cttcgttcac atcggggatc ttttctgttt caaaaatcaa tgtgccggtt
    57781 tctgcttctt ttgaataaac ttttccgaca taccattctt gaccatcgat cgtctcgact
    57841 tttaggtctt ctccatcttc atcataggag gatagacctg ttccacgaac gttttttacg
    57901 gtaccaatcc cggcggctgc cgcatcgatt gctaaacgta ttctaccttc attggcactt
    57961 tccttttttt caaaagccag atgccattca agggtttctt ctttttcaat atacttccct
    58021 tcaatttttg catagtcctc atctaagaat acctcatcct cctttgtttc tgttgactca
    58081 gcagctacat ccagggtcgt catatgatag actgactgga aatatggtcc aaatagcaaa
    58141 ctgacagtta ctaactgcaa aagcttcaat tttaaccctt tcacatcctt atactccttt
    58201 caaaaatatt tgtcttcttt cacaacaatc aacaaaacaa tgcccatcta attataattt
    58261 aatttaattt taattttaaa tcaaatgata tcctttttag ttaaaaaaca ccaattatac
    58321 aacatttatt attaaaatta aattataaaa ttttataacg ttctcattta acaaaactta
    58381 agttattaag acaaatgaat aaacaactac gctttcctcg aaacaaaaaa ataaagcaca
    58441 aatgcgcatt tacgcactca tgctttattc gtacaattaa ttcagataac ggcagcgcat
    58501 aggatgccaa taacacttcc taaaatagct cctcctaaaa tgtcagtagg ataatgtaaa
    58561 agtaaaaaaa tcctcgaata agcgatcaaa caagcaaata aaaaggctaa tattcctgca
    58621 actggactta attcgaacaa cgcagctact gataaaaagg ctagcgctgt atgtcctgaa
    58681 ggaaaagaag aagtcttcgg tttacttgtg taaaggacaa gccgacgatt tttttcataa
    58741 ggtcttttcc gtcttgtcgt ttttttgata aaaagcaaac caacaaaaaa atagatcaaa
    58801 ctgccgataa gaatggctag accatattct ctagtatcag gtatcaagac taaaacagca
    58861 gcaaggagta accaaataaa accaccatta gagatattac tgataaattt caaaaaattg
    58921 atttttcgac tatccgtttt ccgttgcttg gcgaacttgt tgataatact atcatcgatt
    58981 ttagcaatca aacgcaacat gttattcctc ctttttaaga accttttctt gcgtacgatg
    59041 gataaaaagc aggacttgaa aataaaacaa gaaactgaaa cctaataaag caaaatagat
    59101 tggtcctgca aaccagaatg cgacaagaag gatcaacgtg cctgccaaat agaaaagcgc
    59161 gcttttgaaa tcattaaaaa acaagaaaaa tgcagtaatc caagaagctt ctttttgttc
    59221 cagttgaagc aagattttgt aactaaagag aagcgcgctt aatagaagca aaaacaacgt
    59281 agtatacatc aatgtatagc caattccccc accccaatag cggaaaatct gataatcgat
    59341 caatagaaga agaaaataaa cacttgctaa actatcttgc tttccataaa gagagaagtt
    59401 ctcacgaaac tttttccaga atagtgaacc gagctttttc tcttcatttt ttttgtaatg
    59461 ataaatcata tacagaactg ttcgacttgc agttcctaat gtgaaaacag gcaagacgca
    59521 caggaaccat agtagctgta aatggataag acgtggaatc cattcaatga tttctaagaa
    59581 ataatcattg gcttttttac caatcgatcg attcatcatc cttttgaacc tcctgtcaaa
    59641 tccatccctt caatgaaata acgttgtgca aaaaagaaca gcaaaatcgt cgggatcaag
    59701 acaagtgttg ctcctgccat cagataattc cattgtgtcg ttgcttggtt ctgaaaaact
    59761 tgcaagccaa tctgtaacgt gtatttttct tgatcttgga tatataacag aggacctaag
    59821 aaatcattcc aagctgcgtt gaaggaattg atcgcaatgg taatcaaggc aggtttcgtc
    59881 aatggaagca tcaaatgact ccaaatatac aaatgattcg ctccgtcgat ttcagcagct
    59941 tcgatcaatt cattattgat cgataagtaa aattgacgca tcagaaatat attgaacgca
    60001 ttgccgaaga acgatggaac gatcaggggc aaataagtac ctacccagcc gatcttggag
    60061 aataatacat attgagggat catcgttaca aaccccggga tcatcatcgt agaaagaacc
    60121 aaagcaaaca tcagcttttt cccaggaaaa tcaagcttcg caaatccata agcaataaaa
    60181 gaatttgaaa ggacattacc gatcactact agcacagtga tgaataatgt atttcttgca
    60241 tagcgccaaa aaggaaaggc ggcaatcgta tccagatagt tttcccatac gattttttct
    60301 gggaaaaagg tcggaggata acgaacgatt tcttccattg gtttcaatga agtaaacacc
    60361 atccaaagca atggaatgat caaaatgatt cctattactg tcatgaggac ataacttagt
    60421 attttttttg tttgccgatt cacactggtc tttttgaaat aatccatcat ttgccgtcac
    60481 ctccagaata atagacccac ttaggtgcaa gcttcaaatt gacaaacgtc agaaccaaca
    60541 cgatcagaaa caggatcatt gacatagcca tcgcataacc catatcaaaa gctacgaacg
    60601 ccttgttcca catatgcaga ttataaaaaa gcaaagaatt tgcaggtccg cctgttccat
    60661 ttttcgtcat gacataagct tcttgaaaaa tttggaaaga accgatcgta gaggtgatca
    60721 catcgtaaaa aataatagga gtgattaatg gcaaagtaat acttttgaac tttccccaaa
    60781 cgcctgctcc atcgatttct gctgattcat ataaagatct aggaacattt tgtaatgtag
    60841 ctaagtaaag tagcattgct cctccagaac tccatatttt catcaaaatc aacgcaggtt
    60901 tcgtccattg tggatcaaac agccaagctg gtccagggat atggaaccag cccagaaatg
    60961 tgttgatcaa tcctgcagat ggagaaagca gctgcatcca taagatgtag actgctactc
    61021 ctgataagac tgccggtaaa tagaaagctg tacggaatac actgattcct ttgatttttt
    61081 gattgagtac tgtggctaaa aacacactga aaatcgtcgt cagcggtaca ttgaagatga
    61141 cgaaataaac ggtgttgtat aacgatttcc aaaataaatc atcgtgaacg aacattcttt
    61201 tgaaatttgc tagaccaatg aaattcatct tggatgtcaa attataatcg gtgaaactac
    61261 caataactga aaaaagcata ggccctagca taaataccaa gaacccgata ataaaaggcg
    61321 agataaataa ataggcccat cttgcttctt ttttctttcg gatagataaa cgcttcaaat
    61381 tttcttgcct cctctgtacc tagacagtca aaaatggatt attgattttg cttgactaag
    61441 ttttctactg ctttttgtgc atctgccaat ccttcttttg gtgattttgt tcctaaaaga
    61501 atctggtcaa tctgttcatt cactaatgtc gtgtaatccg gagcatctaa aggaactggt
    61561 gtgatcaccg tatttttaaa gttttcgtcc gccatttgat agatcatttt tcctttatcc
    61621 gttaggttgt cattattcac taaattctca ttggctgtct tgctcgccat gatatcaaaa
    61681 cttttttcgc cgaatttttc ttgtacttct ggagaagaaa gatatttgat gaattcataa
    61741 gaggcctcag gatcttttgc tccgtatggt acttccaaga cgaatccgcc accccacgac
    61801 caatggcctg aaccttcttc tttttctggg agctgcatca cgccaaaatc aaaatcatcc
    61861 ggtgcatttt ccatcaaact agagtaataa ttgatatttt gggcacgcat ggctactaat
    61921 ccggaaataa atggatcaga tacaccagac ccaaattcgg cttctaattt attgatcgta
    61981 tttttcccat aataatcttg ccagtcttgg atccactcca aggcttccac tttattttta
    62041 gtatcaatct tgactttttc atctttgtca aaccaactag tcccgttatc tgcgtttagc
    62101 gcccatacat cagcccctaa attccataat ggataaaaac cgatgcgtgt aaattctttt
    62161 ccttcttttt tatctagttt acgtgctgtt tcttctaatt catcccaagt tgtcggtaga
    62221 tcttgttcac tgattccagc ttctttaaat aactttttat tataaaaaat gacttgtgta
    62281 tatgtattga atgggatcgc ataaggatca ccttcatatt ctactgtatc ccaaagctgc
    62341 ggataaaaat ccttagaagt gtctttttct acatatttgg agagattagt tgcttgctgt
    62401 gcttctgctc tttgggcaac ggaatttatg tcctgtacaa taacatcggg tgcatttcca
    62461 gctgttattg cagataaaga tttagtccag atgtcacccc atggctgata cttataattt
    62521 acatggatct cgttttgtga ttgattgaaa tcctcaatga tttcttcgat agcttctttt
    62581 cgtgcaccgg atccccaaaa ggaccagaaa tcaatatttt ttcttccatc acttgttttt
    62641 tcagaagaat ttgctgcact tgaactggca tcgcttgcat tacttgatcc gcatcctcct
    62701 aataaaaaca atgctgaact tgccagcaac cctgtaaaca ataatttttt catattttac
    62761 ctccaaattt taatgtaacc gatgtcggtt ttaatataac gatatcattt tgttctgaaa
    62821 ataacaattt ataaggtatg attctaaaaa aattattaat gataccaata ctttatataa
    62881 ttcgcaagcg ttttctttga attttttaat ctttcgtagc agaatgagaa tgtattaaaa
    62941 atagattagg aggaattttg atgtttagac accagaaaga attgcagttt gaagcaaaac
    63001 cagatcgtcc gaatccatta atcgcgaaat atctgcagga gctgatcggc ggccaatatg
    63061 gcgagatgag tgtagctatg cagtacctgt tccaaggctg gagctgccga ggagaagaaa
    63121 aatataaaga tatgctgatg gacattgcaa cagaagaaat cggccacgtg gaaatgttgg
    63181 cgacaatggt tgctcgttta ttagagggtg cgcctgttga agatcaagaa gcagcgatgc
    63241 aaaaagatcc aacaatcggt gccattatct ctggaatgaa tccacagcac gcgatcgtag
    63301 ctggcttagg acctcgtcct gctgacagcg tgggcaatcc atggagcggt gcctatatca
    63361 ttgcgagtgg caacttgtta gcagacttta gagctaattt aaatgccgaa tcacaaggaa
    63421 gacttcaagt agcacgtatt tacgaaatga tcgatgatcg tggcgtaaaa gatatgctgt
    63481 ctgttctcct tgcaagagat agctatcatc aaaatctatg ggaatgtgca gtgaaagaat
    63541 tagaagaaaa agaaggttca atcttagtcc caagtacttt cccgcgtgac aaagaacgaa
    63601 aagacattgc ttatgacttc tataacttct cagagggaga ccaaagtagt gaaggttctt
    63661 gggctagtgg caaagcactg gatggcgaag gcgaatatca atggttgaaa gaacctaaga
    63721 tcgaaggcgc tgcaccaaaa ttagaaccag taacaccaaa aatgtatggg acacctccat
    63781 tcaagaaata aatatctacc cgtagaaaca cccgtttttt gttccacacg ctttgtgagc
    63841 aaaactgact aaagtcatta atcataacca cccttcaaaa gggtggtttt ctctagagct
    63901 ataagctcaa atcaccggcc agcgcctaaa gacgctggct ttcatatttt atgttcaagc
    63961 catattgcct ttgccagttt tgcccacctc ttaaaaggtt tatgtactac tccttagccc
    64021 ttaaaaggat cgtcttactc tcgtacactc agcttatcta acgcactatc atgtttttct
    64081 tgttcttgaa tgtacttttt gatggtcgct tcattttgtc ctacttttgt cacatagtat
    64141 ccttccaccc aaaagtgtcg atacccaaat ttatatttta gatttgcaca atctcttgat
    64201 gatctcctga atattggctc gatattgatt ataaattatt tttcttctat actttggtgt
    64261 gaatacaatg tggtatttac acatctactt tgtacgggct aaactatttc cctttattgc
    64321 tccacccgca tagcaagtgg ttttgtattt caatcgctcg gcgcgctcaa ttaggtctaa
    64381 agacaataaa tctaaaaacc cgctgcttca ctgacagaaa caacgggttt tcagaaaaaa
    64441 atgaaatgaa aaagatctct tacggcaatc tattttattt ttttgatttt ttataaatgg
    64501 aaaatgcttc aagaagtcca agaaataaag ccgtataaaa accagaacct atgaaatctt
    64561 tatttattag atattcaaca gaaatcccga taacactagc caacaatata tttatcagaa
    64621 gtgtaaaata aggatgcttt ttatagaata gaactagtgt tgttttcata gactctcctt
    64681 tcttcatatt tccaattaat cacaaattca cggcattact agaaaaatta aaggggcgca
    64741 gaagtggaaa aatactcttc gactttccct acaaaccagg ccatcttgtg tttaataact
    64801 ataccaaatc taaggtacct ctaaatctaa attccatttg tggcggtgac ctccaatttt
    64861 taactcttct atctcccatc cagtatacat aaccttgata cttcgaccac ttatatatcg
    64921 aacacacaaa tacctatcca ttgcaggagt actacctaac ttagttgcta cttgcatcta
    64981 aataacagct gaaaagatag gacttactct tagtaaagta tctgaactat taatttttaa
    65041 actatcattt ttatcctcaa ctacatgcac atctgttgca acttcacttt ctgtctcatc
    65101 cagataaatg attaaggaat cgggaattgc aatatcttca gctgacgctg gaatagttga
    65161 gctaaattag tcatcatatc tttattataa aataaccaac agcaaaaaat acaatgaaaa
    65221 acaaacttgg aactaatgta tcattaataa atgttgaaaa attcagcaac tcattactgt
    65281 atgattttag cagcccaacc aacatatacc ctataaaagc taataagatg gttaggaatc
    65341 ctttagaatt tttactcatt catagacact tccttaa
//
LOCUS       DACDPS010000007        64551 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.007, whole genome shotgun sequence.
ACCESSION   DACDPS010000007 DACDPS010000000
VERSION     DACDPS010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 64551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.007"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            11..604
                     /locus_tag="IWJ07_000500"
     CDS             11..604
                     /locus_tag="IWJ07_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP9332105.1"
                     /translation="MGTIVVVLGLLGLLFAPSIISIFSLTITGFIAFTLVFGKKDTKK
                     MFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPINEAFSPLLFIILPIM
                     ILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNGNILHTVAHILLIQGV
                     ARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(723..795)
                     /locus_tag="IWJ07_000501"
     tRNA            complement(723..795)
                     /locus_tag="IWJ07_000501"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(760..762),aa:Thr,seq:cgt)
     gene            916..1158
                     /locus_tag="IWJ07_000502"
     CDS             916..1158
                     /locus_tag="IWJ07_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332106.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            complement(1297..1926)
                     /gene="upp"
                     /locus_tag="IWJ07_000503"
     CDS             complement(1297..1926)
                     /gene="upp"
                     /locus_tag="IWJ07_000503"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAP9332107.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(2060..3007)
                     /locus_tag="IWJ07_000504"
     CDS             complement(2060..3007)
                     /locus_tag="IWJ07_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9332108.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            complement(3160..4404)
                     /locus_tag="IWJ07_000505"
     CDS             complement(3160..4404)
                     /locus_tag="IWJ07_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAP9332109.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            complement(4579..5604)
                     /locus_tag="IWJ07_000506"
     CDS             complement(4579..5604)
                     /locus_tag="IWJ07_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAP9332110.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            complement(5626..5805)
                     /locus_tag="IWJ07_000507"
     CDS             complement(5626..5805)
                     /locus_tag="IWJ07_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332111.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            complement(5798..6637)
                     /gene="prmC"
                     /locus_tag="IWJ07_000508"
     CDS             complement(5798..6637)
                     /gene="prmC"
                     /locus_tag="IWJ07_000508"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAP9332112.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            complement(6630..7703)
                     /gene="prfA"
                     /locus_tag="IWJ07_000509"
     CDS             complement(6630..7703)
                     /gene="prfA"
                     /locus_tag="IWJ07_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAP9332113.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            complement(7706..8290)
                     /locus_tag="IWJ07_000510"
     CDS             complement(7706..8290)
                     /locus_tag="IWJ07_000510"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP9332114.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            complement(8396..8865)
                     /locus_tag="IWJ07_000511"
                     /pseudo
     CDS             complement(8396..8865)
                     /locus_tag="IWJ07_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8971..9573
                     /locus_tag="IWJ07_000512"
     CDS             8971..9573
                     /locus_tag="IWJ07_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP9332115.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            complement(9677..10246)
                     /gene="lepB"
                     /locus_tag="IWJ07_000513"
     CDS             complement(9677..10246)
                     /gene="lepB"
                     /locus_tag="IWJ07_000513"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP9332116.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(10476..11243)
                     /locus_tag="IWJ07_000514"
     CDS             complement(10476..11243)
                     /locus_tag="IWJ07_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332117.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            complement(11240..11719)
                     /locus_tag="IWJ07_000515"
     CDS             complement(11240..11719)
                     /locus_tag="IWJ07_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAP9332118.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            complement(11844..12581)
                     /locus_tag="IWJ07_000516"
     CDS             complement(11844..12581)
                     /locus_tag="IWJ07_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAP9332119.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            12742..14142
                     /locus_tag="IWJ07_000517"
     CDS             12742..14142
                     /locus_tag="IWJ07_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAP9332120.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            14365..15717
                     /locus_tag="IWJ07_000518"
     CDS             14365..15717
                     /locus_tag="IWJ07_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAP9332121.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            15710..16408
                     /locus_tag="IWJ07_000519"
     CDS             15710..16408
                     /locus_tag="IWJ07_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAP9332122.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(16470..17621)
                     /locus_tag="IWJ07_000520"
     CDS             complement(16470..17621)
                     /locus_tag="IWJ07_000520"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAP9332123.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(17634..18071)
                     /locus_tag="IWJ07_000521"
     CDS             complement(17634..18071)
                     /locus_tag="IWJ07_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9332124.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(18086..19852)
                     /locus_tag="IWJ07_000522"
     CDS             complement(18086..19852)
                     /locus_tag="IWJ07_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAP9332125.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            20116..20793
                     /locus_tag="IWJ07_000523"
     CDS             20116..20793
                     /locus_tag="IWJ07_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9332126.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            20796..21923
                     /locus_tag="IWJ07_000524"
     CDS             20796..21923
                     /locus_tag="IWJ07_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9332127.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(22123..22563)
                     /locus_tag="IWJ07_000525"
     CDS             complement(22123..22563)
                     /locus_tag="IWJ07_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9332128.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(22574..24607)
                     /locus_tag="IWJ07_000526"
     CDS             complement(22574..24607)
                     /locus_tag="IWJ07_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9332129.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(24609..26312)
                     /locus_tag="IWJ07_000527"
     CDS             complement(24609..26312)
                     /locus_tag="IWJ07_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9332130.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(26406..27356)
                     /gene="manA"
                     /locus_tag="IWJ07_000528"
     CDS             complement(26406..27356)
                     /gene="manA"
                     /locus_tag="IWJ07_000528"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAP9332131.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            complement(27395..28420)
                     /locus_tag="IWJ07_000529"
     CDS             complement(27395..28420)
                     /locus_tag="IWJ07_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP9332132.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(28629..29498)
                     /locus_tag="IWJ07_000530"
     CDS             complement(28629..29498)
                     /locus_tag="IWJ07_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAP9332133.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(30202..32094)
                     /locus_tag="IWJ07_000531"
     CDS             complement(30202..32094)
                     /locus_tag="IWJ07_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9332134.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(32091..33818)
                     /locus_tag="IWJ07_000532"
     CDS             complement(32091..33818)
                     /locus_tag="IWJ07_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9332135.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(33834..34484)
                     /locus_tag="IWJ07_000533"
     CDS             complement(33834..34484)
                     /locus_tag="IWJ07_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9332136.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            34688..34984
                     /locus_tag="IWJ07_000534"
     CDS             34688..34984
                     /locus_tag="IWJ07_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP9332137.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            35176..35796
                     /locus_tag="IWJ07_000535"
     CDS             35176..35796
                     /locus_tag="IWJ07_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAP9332138.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(35888..37054)
                     /locus_tag="IWJ07_000536"
     CDS             complement(35888..37054)
                     /locus_tag="IWJ07_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9332139.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(37066..37419)
                     /locus_tag="IWJ07_000537"
     CDS             complement(37066..37419)
                     /locus_tag="IWJ07_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP9332140.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            37547..38569
                     /locus_tag="IWJ07_000538"
     CDS             37547..38569
                     /locus_tag="IWJ07_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAP9332141.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(39008..39442)
                     /locus_tag="IWJ07_000539"
     CDS             complement(39008..39442)
                     /locus_tag="IWJ07_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332142.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(39552..41411)
                     /locus_tag="IWJ07_000540"
     CDS             complement(39552..41411)
                     /locus_tag="IWJ07_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP9332143.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDHSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(41529..42689)
                     /locus_tag="IWJ07_000541"
     CDS             complement(41529..42689)
                     /locus_tag="IWJ07_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAP9332144.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(42996..44420)
                     /gene="araA"
                     /locus_tag="IWJ07_000542"
     CDS             complement(42996..44420)
                     /gene="araA"
                     /locus_tag="IWJ07_000542"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAP9332145.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(44436..45134)
                     /locus_tag="IWJ07_000543"
     CDS             complement(44436..45134)
                     /locus_tag="IWJ07_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP9332146.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(45091..46752)
                     /locus_tag="IWJ07_000544"
     CDS             complement(45091..46752)
                     /locus_tag="IWJ07_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAP9332147.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            46970..48370
                     /locus_tag="IWJ07_000545"
     CDS             46970..48370
                     /locus_tag="IWJ07_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAP9332148.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(48622..49701)
                     /locus_tag="IWJ07_000546"
     CDS             complement(48622..49701)
                     /locus_tag="IWJ07_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9332149.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(49787..50746)
                     /locus_tag="IWJ07_000547"
     CDS             complement(49787..50746)
                     /locus_tag="IWJ07_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAP9332150.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(50758..52281)
                     /locus_tag="IWJ07_000548"
     CDS             complement(50758..52281)
                     /locus_tag="IWJ07_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP9332151.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(52291..52854)
                     /locus_tag="IWJ07_000549"
     CDS             complement(52291..52854)
                     /locus_tag="IWJ07_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP9332152.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(52860..53723)
                     /locus_tag="IWJ07_000550"
     CDS             complement(52860..53723)
                     /locus_tag="IWJ07_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9332153.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(53737..54633)
                     /locus_tag="IWJ07_000551"
     CDS             complement(53737..54633)
                     /locus_tag="IWJ07_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9332154.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(54808..56079)
                     /locus_tag="IWJ07_000552"
     CDS             complement(54808..56079)
                     /locus_tag="IWJ07_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9332155.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(56069..57586)
                     /locus_tag="IWJ07_000553"
     CDS             complement(56069..57586)
                     /locus_tag="IWJ07_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP9332156.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLNLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            57784..58674
                     /locus_tag="IWJ07_000554"
     CDS             57784..58674
                     /locus_tag="IWJ07_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAP9332157.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(58747..59571)
                     /gene="nadE"
                     /locus_tag="IWJ07_000555"
     CDS             complement(58747..59571)
                     /gene="nadE"
                     /locus_tag="IWJ07_000555"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAP9332158.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(59583..61055)
                     /locus_tag="IWJ07_000556"
     CDS             complement(59583..61055)
                     /locus_tag="IWJ07_000556"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAP9332159.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(61692..62054)
                     /locus_tag="IWJ07_000557"
     CDS             complement(61692..62054)
                     /locus_tag="IWJ07_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP9332160.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            62277..63029
                     /locus_tag="IWJ07_000558"
     CDS             62277..63029
                     /locus_tag="IWJ07_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9332161.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(63169..64092)
                     /locus_tag="IWJ07_000559"
     CDS             complement(63169..64092)
                     /locus_tag="IWJ07_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9332162.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(64107..64379)
                     /locus_tag="IWJ07_000560"
     CDS             complement(64107..64379)
                     /locus_tag="IWJ07_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332163.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        18586 a        13294 c        11265 g        21406 t
ORIGIN      
        1 aaaagggata ttgggcacta ttgtcgttgt actcggtttg ttagggttgc tatttgcgcc
       61 atccattatt agcatttttt ctttaactat cactggattt atagcattta cacttgtttt
      121 tggtaaaaaa gatacgaaaa aaatgttctc gaaacctgtc gctcctgtca aaaatatagc
      181 caaatatttt tttatcaatg tagtaatcag tgccgcagtc tcggtatttt tgcaacaaat
      241 tttgaaatgg ggtttaacag gcaatccaat aaatgaggct ttcagcccgt tattatttat
      301 tattttgcct atcatgatac ttggagaaga attgttctca gtgtattttc tcgctatttt
      361 ttctagtaag ttcagcattc ctgtcgctag tttcttgtca gcaatcatct ttggatttat
      421 ccattacagt acttatgata atggaaatat tttacacact gtagcacaca tccttttgat
      481 acaaggggtg gcaagattgt tattcaacca agctgccatt aaaagcaact cgatcattac
      541 aagttgggca gttcatgtga tatttgattt cacagctata ttgctagtcg ttttattctc
      601 ctaaattttt atagaaataa tacatgctct attgctaaga aagcatactt tatttccttt
      661 aaacaaaaag acagatgtat caatggatta aaaatccttg atatatctgt cttctctttt
      721 aatgccagct atagggatcg aacctacgac ctacgcgtta cgagtgcgtt gctctaccaa
      781 ctgagctaag ctggccttat ccaaaagaaa ttatatactc tatttggctg attgtaaaga
      841 gtccgatgga aaattagttt aatttcatga tatttttcat aaaacgtggt ataattgatg
      901 tgaaaggcag gttttatgaa aatggcaact tttggaaaat ttgatacaac tatcgtacca
      961 tcggaaattg gtgaaaaatt caagatcaat actcacgtaa tgttcacaat tcacggcaag
     1021 tcgcaaactg gaacgatttc aaagcaattg aaaaattccg ctgttgttga gattgatgaa
     1081 actgcagaaa atgcgaactt gatgacgcaa agtaagggtg tagtaatcgt taactacaaa
     1141 gaactaaaaa agatttgaat ttgaccatct ctgcattttt gggatggttt ttttatatgc
     1201 aaacgaaagc tgtgaaaaag ctgttctgca tcaaacgtta aaaatgacca aaaaaggatg
     1261 tgacaaaagt tgtgtcacac cctttttcgt atttacttat tttgtaccaa acaaacggtc
     1321 acccgcatcc cctagtccag gaacgatata accatgttca ttcaattttt catctagaga
     1381 agctgtatag atgtcaatat caggatgcgc ttcttgcaaa gcttttactc cctcaggtgc
     1441 tgctaccaaa caaacaaatt taatgttcga cgcgccacgt tctttcaatg catcgattgc
     1501 catgattgct gatccgcctg ttgccaacat tggatctact acaaacaact gacgtgcatc
     1561 aatgtcttct ggaagtttga cgaaatattc atgtggttcc aatgtttctt catctcgata
     1621 aagaccgata tgtcccactt tagcagctgg aatcaattcc aagataccgt caaccattcc
     1681 aatacctgcg cgcaagattg gaataatcgc tactttttta ccagacaacg ttttttgtgt
     1741 tgtttcagtg atcggtgttt cgatcacgat gtcttccaat ggcatatccc gtgatacttc
     1801 atatgccatc aacatagcga tttcatcgac tacttcacga aaaacctttg ttccgcagtt
     1861 tttgtcccga ataatagtta gtttgtgttg aatcaatgga tgatcgatca cttgaaattt
     1921 gcccattttt taagctctcc tttgttctgt cgtttctttt taattgtaaa acaaaatcaa
     1981 gtgattgact agtcaaactc gccatttttc ctaagaaaat ccgtttttga aaagaagttt
     2041 tcctgcggac aagcaaagct tattcttttt tcttgatcca agaagtagat aatgccattg
     2101 ctgcacgcgt tgcctttgtc tgatccatag catttaccat gacaaaatca tggatgattc
     2161 cttggaagcg tacttgtgta acaggaaccc ctgcatcacg aagtttgcgc gcataagcct
     2221 caccttcatc tcttaataca tccgcttctc ccgttaaaat cattgctgaa ggaagatcat
     2281 ttagttcatt tatatttgct ctcaaaggcg aagctgtgat ttcagcacgc tcttcttcat
     2341 tcgtcgtata ttggtcccaa aaccacatca ttccttctct tgtcaaaaaa tagttttctc
     2401 cgaattcatg ataggaatct gtatcaaaat cagcgtttgt gactggataa tacagcaatt
     2461 gcttggatat tgggaggccc tttcgttctt ttgttaagat cgtcatgact gtagccatgt
     2521 taccacctac tgaatctcct gcaacgatta gattctcgag atccagattt tttgacttgg
     2581 ctaatttatt cagctgttgc aacacagcat aattttgttc aattgctgtt ggatattttg
     2641 cttctggtga tcgactatat tctggaaaga caacgacgga attcgtacga accgcaagtt
     2701 ctctgatcaa tttatcatgg gtttgagcac tgccaaaaac ccagcctgct ccatgaatat
     2761 aaaaaataac tggtaattta tccatattcc cttctggtcg tacgagacga atattgagtt
     2821 ttccccattc gccagtatcc accatcaagt cttcaatatc gacggaatat ttgttcacag
     2881 gtgattcttg tacttcttct agtaaaactc ttccttcttc cggtgctagt tcatagatac
     2941 gcggatgagg cgcatttgca tcactgaatg ctactgcttc cttttctaac gagatacgat
     3001 cagtcatgac ttttcctccc tgagatttag tgtgaagact aaaattacac ctcatatact
     3061 agagagcatc accgatattg tcaaagaatc cgttttccca aaaaataaac agaaaagccc
     3121 aacattgaac ttttctgttt actaatggaa tttaaatttt tattttttat gcaacggaaa
     3181 tttttcagtt aattcgcgta cacctgtttt gacttcgtct agttgagcgt tatcatcttt
     3241 tgcttgtaat gctttgacaa ctagttcagc cacttttaca gcatcttctt ctttgaaccc
     3301 gcgagtagtg attgctggcg tgccgattcg aataccgctt gttttgaatg gacttagtgt
     3361 ttcaaaagga atcgagtttt tatttacagt gatattcaca ctgtccaaaa tgctctctgc
     3421 ttcttttcca tttataccta actcgcgaac atcgatcaac atcagatgat tatctgtcgc
     3481 ccctgaaatg actctggttc caattgcttg attgaaaact ttgaccattg cttttgcatt
     3541 ggcgatgatt tgttcactat attctttaaa tgcaggatct aatgcttctt tgaatgctac
     3601 tgctttaccc gcaatcacat gctctagagg gccgccttga ataccaggaa ataccgcact
     3661 atttattttt tttgctaatg cttcatcgtt tgtcaaaatc atgccgcctc gagggccacg
     3721 caatgtttta tgagtggtag tcgttgtgat atctgcataa ggaactggat taggatgaag
     3781 tcctgctgct actaatcctg cgatatgtgc catatctacc atcagttttg cgcctacttc
     3841 atctgcgatt tctctgaatt tcgcaaagtc gatcgttcgt ccgtaagcac tggcacctgc
     3901 tacgatcaat tttggttgat gttttctagc taaaatacgt accacattat aatcaataac
     3961 ttctgttgtc ggatcaacac cgtaagcaac aaagtgatag gttttgccac tgaagttgac
     4021 tggtgatcca tgtgttagat gtccgcctgc ggaaagatcc attcctaaga tcgtgtcccc
     4081 tggttctacc aaagcgagat aagcagccgt gttggcttga gagccggagt gaggctggac
     4141 gttcgcaaat ttcgctccga ataattcttt tgcacggtca atcgctaaat tttcaacgat
     4201 atcgacaaat tcacacccac cataatagcg atgccctgga taaccttctg cgtatttatt
     4261 tgtcaagata cttccttgtg cagccatcac tgcttcggag acaaagtttt cagaggcaat
     4321 cagctccaaa ttatgttctt gtctttcttc ttctttggcg atcgcagccc aaagatcagg
     4381 gtcaaacgtt ttgtaatcta ccacaaacaa cagctccttt ttattttcct acaattttat
     4441 ttttcccatc aaaaacaaat tgccaaatat tcgtatctct tctattacga ttagttacgc
     4501 ttagtttttc gttcactgat agaagattta cttgatagca ttgtatcata ctaaaagaat
     4561 gcgacaataa ccaattgatc atttttcaaa ataattttgt cctgctgctt ttttcaagcg
     4621 attcatgtaa gcattcccca atccagtttc tggatacact tgtacaaaaa tcaaatctaa
     4681 tccaagtgtt ggttcatcta atcctcttag acctgcaaac aatcctttag ccgccgcttc
     4741 tactgagttg tcgttataca tataaacagc tgcagtatct gtccgcactt gatccgcaat
     4801 ttcggggctt gctatgacac ctgcacggat cttcttattt tttgcccatt gaactgcagt
     4861 tgaccaatcg ccatctctga ccatcagaac acgtgtatcg ggtgagtagt gtttgtattt
     4921 catcccaggg gcttttggtg tttcattttc ttttacaaga tgctgatcaa tagcaacagg
     4981 agattcgatg actgcttcga tttgttcttt cgttactgca cctggtcgta aaatcatagg
     5041 catggctgtt ggatcactta aatcaagaac agtggattct acaccaattc gcgtagctcc
     5101 atcatcaata atcccggtaa tttttccttg tagatcatga tatacatgat ctgcagtcgt
     5161 tggacttggt tttcccgacg tatttgcact tggaccgaca agcggaacac cggaaagttc
     5221 gattacttct agtgtttttt tgttatccgg tatacgaaat gcagctgtgg ataatcctcc
     5281 cgtcacaact gagggcaaag tgtctttttt gatcttaaat atcaaagtta atggtccagg
     5341 ccagaagttc ttcacaatct tctctgctgc cggatgaaag ttgtccacat actcttttac
     5401 ttgttcaaaa gaggcgacat ggacgattaa tggattatct tgcggtctcc cttttacaga
     5461 aaacactttc ttgacagcgt ttggtaaaag agcgttggcg cccaatccgt atactgtttc
     5521 ggtaggaaaa gcaacaagtt ctccttcttt cagtgcttcg gctgcctctt gcaattctgt
     5581 ttcatcatat cgtttcgttt ccacagttta tccacaaccc ctttttcata cattcttatt
     5641 ttgtctgagt ttatcctcaa gttttccact gtttgtggat aatctatttg tcaatagtgg
     5701 ataaacttgt tgataaacca aaaagtcgtt tacatccttg tcaaataagt gtttttttat
     5761 aataaaaaaa caaataaaaa atttttcatc cacaacttca accataaact tggatcattc
     5821 gttcattgcc aaaaagatcc ttttttatcg ccactttttt atctggaaat gcttgctgaa
     5881 atattttttt tactgcttca ccttggcgaa atccgatttc taaaaaaatc tttccatttg
     5941 attttagtac gcttggtgct tcaactgcta ttttttcata aatagctaat ccatcgttct
     6001 cagcaaaaag ggccatttta ggttcgaatg accggacact ttcatccatc agttcccatt
     6061 cattccgact gatgtacggc ggattagaaa taatcacatc aaattgctga tccattactg
     6121 gtcctaatgt atctccatga taaaaactga ttttcgttcc ttctttctga gcattttctt
     6181 tcgctacttc taatgcttct tctgaaagat ccacagcaga tatatgccaa ttttttcttg
     6241 ctgctttcaa actaatagca attgctccag tccctgtccc aatatctatc acttctaacg
     6301 gtacaccagg cgtctcgtct aaacaccatt cgactaactc ctctgtttct ggtcgaggga
     6361 tcagtgttgc ttcagttacc tttaaccgat gatcataaaa atctgcatat cctagcaaat
     6421 actgaggtgg atgatctgca agcagaagtc gcagatcggt ttcgatcaac cgctgttctt
     6481 cttctgaaat ctcttcgttc atatgaagga gccaatcaag ttttttccat tgttttcgtt
     6541 ccaaaaataa aaattggata ctatatgctt ctttcccatg atcctctaaa aaagaagaag
     6601 cccgtgacag gacttctcga taggtcattt tacccatttt gcatagcttc cagttttgat
     6661 gtttgatcat acatgatcaa tgcatcaacg atctcatcta atttccctgc taaaatctga
     6721 tctaattttt ggatagttaa tccgatacga tgatcagtca cacgattttg cgggaaatta
     6781 tacgtccgga tccgttctga acgatcccca gttccgactg ctgattttcg attggcatca
     6841 tactcgcttt gtgcttcttg ctggatctta tcgtacacac gagcacgcaa gattttcatt
     6901 gctttttcac ggttcttgat ttgagaacgt tcatcttgca tagcaacaac gatccctgtt
     6961 ggtaagtgag tcaaacgtac agcagatgct gttttattga cgtgctgtcc gccagcacct
     7021 gaagcatgat agatatctgt acggatatct ttgtcttcaa tatcgatctc tacttcttca
     7081 gcttctggca tcacgacaac tgttgcggtg gaagtatgga tacgcccttg cgattcagtt
     7141 gaaggaacac gctggacgcg gtgcgcacca ctttcatatt ttaatttcga atatacgttg
     7201 tctccggtga tcatcatgat cacttcttta tatccaccga ttccggtaac gttagcttcc
     7261 atcacttctg ttttccagcc ttgtgcttca gcatactttt ggtacatatt gaaaagatcg
     7321 ccagcgaata atgctgcttc gtctccgcct gctgctccac ggatttccat gatgatattt
     7381 ttatcgtcat ttggatcttt tggtaaaagc aagatcttga ttttttcttc taagacttct
     7441 ttttcttttt ttaattcaga taattcttct tttgctagtt cagccatttc atcatccagt
     7501 ttttcaccaa gcagttcttc tgtatcggtg attccttgta ctacttcttt ataacgacga
     7561 taaacttcga ctgtttcgcg cgtgtttgct tcttcttttg aaagctgcat aaaacgcttt
     7621 gtatcagaaa tcacttctgg gtcacttaaa agttctccaa gctcttcata acggtcttca
     7681 attgcttgta attgatcgta catgcttatt ctccttctgt aatcattgat gggtgaaagt
     7741 aatgttttcg acaaacgggg taataagctt catttccgcc aatttgcact tgatcgcctt
     7801 cataaacagg gtttccatca atataatgta aattcatgat tgcttttttg tggcagaacc
     7861 aacagattgt tttcatttct tcgattttgt ctgcataaag caataaatat tttgatcctt
     7921 caaataactc gtttcgaaaa tcatttttca aaccaaatgc cataaccgga atatccaatt
     7981 cgtccacaat tctcgtaaaa gcaatgacat gtttcttttt taagaattgc gcttcatcga
     8041 tcagcacaca atagggtttg ctttccattt tctgaattac gtcaaatacg tctgtttctt
     8101 caaatatcgg catcgcatta cgtttcagtc cgattcggct tgaaacaacg cctaaaccat
     8161 ctcttgtatc aataccgctt gtcattaaga caactggctt attttgttct tcataattat
     8221 gtgcgacttt taggatctcg atcgtcttgc cgctattcat tgcgccatac ttaaaaaata
     8281 gttgtgccat gctctttttc accttccagt ttgttcctat ggtattatac ttgattttca
     8341 gcatttttta cagtgagaat tttcatcaag cacagattag tattaaatct attgctcact
     8401 tttgttccaa ttttttgaca agaactaaac atgggttcca ttcatcccat aaatttggaa
     8461 aaacttccaa ccttgaaaat cctaatgatt cgtagaaaca aatagtttga tcatatatag
     8521 agtagtgccc ctcgtctaca gtcttcactt gcaaatattt gtaatgtttt gctgcgtaac
     8581 cttctgcttc tttaaaaagt tttgttccta atccttggtg atgatagtct ttttttattc
     8641 ccatacaata gaatagattt cggcagtatc ttcacttgtc tgtgaaagag tgataaagcc
     8701 taaatattct tcttttcttt tttcacacca taaaggtagt ttacttgatt cctcgatata
     8761 tttttccgtt gattcaggaa gcccaaacca ctcaggcaag tctagtaata tttctcggac
     8821 acactttcct ttttcctctc tatcttttac aaacgatatc ggcatattct ttctagattc
     8881 tccctttttg aattgagtat agcatgcttt ctttcttttc acgtgctata ataagttaaa
     8941 atttcaaagt tttacgtaag gagctatttg atgacaaaaa atatttttga ttccatcgct
     9001 aaccattatg attcccctga ccgtcaagca ttagccaaga ttattcgcga agaactaact
     9061 acttatctac cgcgcgacag ccaccaaaaa gtcctgctgg attacggagg cggaactggg
     9121 cttgttagtt taccgcttgc ggaaagattc aaagaactga tcattgccga tgcttcagaa
     9181 actatgctga aaatggcaga agaaaagatc caagcagcag atctgaaaaa tgtccgaacg
     9241 atccacgctg atgcttctgt cgaatttcct gctgtccaag ctaatctcat ccttctatca
     9301 cttgtacttt tacacattcc tgatacggag aatattctta caaaacttta tgaaatttta
     9361 gctcctggcg gtcaattgat catcgtagac tttgacaaga atgaacaaat tagtcatccg
     9421 aaagtccata atggctttag ccaagaagaa ttgaataatc gattggaaaa aacgggcttt
     9481 gtctctgcta ctagtcatac cttccatcgt ggggaaaagc tatttatgaa caaaaacgct
     9541 tcgcttttct tatccatcag ccaaaaagct taaaatgaga ttgcaaaaaa agtgagcaac
     9601 tccaagaaat aagaaggaat tttcggaaaa agaagctgtg acaaaaaaca tgtcacagct
     9661 tctttttatt ttttcatcac atgattttaa tttctgaaag aggccaataa cggaaaacaa
     9721 cttttccttg gatcatatct ttgtcgacta acccaaacat tcggctgtct tttgaaatca
     9781 agcggttatc gcctagaaca agatactttc cttttggcac agtatattca aagtcactcg
     9841 taaaggtgga tgaactttct gcttgtgctt ggaatcctga gctataagca tattctcctt
     9901 gtaacttatc ttctgaaaat tcttttttga actcatccag ataaggttca tcatactttt
     9961 tgccattaat ggtcaactgg tcattttcca tcttgactgt atctccaggc ataccgatga
    10021 tccgtttaac aatcatccgt tcttcatccc ccggttcctt tgtagtaata atatccattc
    10081 gttcatagtt tgagatctta gatgaaatca atcgttgtcc atcacttaat gtagggtcca
    10141 tcgaatgccc gtttacagta actggtgtaa acacgtacac tctagctaat aaaatagccg
    10201 ctaatacaag gatcaaaatc ccccaatttt tcaaaaattc tttcacagct tacctcctac
    10261 ttgtattttc ctcgttattt attacgtttt ttcacaatac ttcgttgtac tttctttatt
    10321 ctacaatgtt tataaatgaa aggaaagaat aagtgatgtg ttcgtcttat ctttctcgtt
    10381 atataaaaaa ggaacgtgac aaaagacgtg tcacatcccc cttattccga ataaacgttt
    10441 ttatcgcaac cactgataca cggcgtttca tcacattatg aatgtcttct tctttggcta
    10501 cgactataaa ccactggttc ttcctgcgac tgtgcctttt ttgcaatcaa tttcagacga
    10561 taaggctcat gatagcgtaa cccgtctttg acaaaatcac cgccgacgat gaacaatccg
    10621 acaggttcca atgtgtcttc tgttgagtca agtcgaatgg tccctacttt ttccatgtcc
    10681 gagtagcttc gtccaatatt tagaacatac tctttctctc ctgttttctt gacatagtaa
    10741 taaggataca atgtatttct atcgaccaga aatccatcca tgcgaccgag ttcatttgtt
    10801 ttgatctcac catttccaat caaataccgc atattctcgg ctaatttcaa ttgataatct
    10861 tcgtcataag aaaccatgac ctcttgtgta ttcactttat cgatcgaatt tccattatct
    10921 atcgattctt ctgtctggat gttgaacgct tcatcaggta aatacttcac aaaaagctga
    10981 gtgacatcgg ctttcgtgtt tttatctaaa aagtaataaa atacatttaa aacagtaaaa
    11041 taaacaagca ctagaaatgc aagtccatat aagaaaaatt gaagatagtt ttggttttgc
    11101 caaagtccat aaacaattct caataaataa agcgtgggaa tcacacttaa aatcgtatat
    11161 ccacggttaa gatatttagg actgatgtct aaatagccaa ggaatttatg caccccgttg
    11221 ataaggtcta gtaaaaatgt cattgagaat cacctgcttg ctggttatac gtttgggtat
    11281 ttcctgaatc ttggttttgc tgtgctgtac gtgctgcatc atcattttgc gtgttatcat
    11341 ctacagtcgt actgcttggt atcgtttcgg agctagcact tgtatccgga cttgtacttg
    11401 gattggttga atccgatgga gtgccatctg gtaccgtatt acttgtttcg cttgagctgt
    11461 cagttgtata tgtctcatca tacgacgaac tagttgttgt atcgtcccat gtacttgtcg
    11521 tagtggtttc attcaaactt tccgttgttg ttggttcaga tgtcgagact gaagtagttg
    11581 gtgttgtttc ttctacagta ctagaacttg aacttggtgc acggtagctg gaactattca
    11641 caatacctgt agtagtcgcg acaatcgtcg agattgttaa tacagcgatt ggcttcaaaa
    11701 taggtgctac ttttcgcatc ttatctcttc cttcattgaa atttattctt gtttccttac
    11761 tttactacgg acaaattata cagtctacct tatttctacc ataaattctt tatttttttc
    11821 ttacgttttt ccgaaagatt tctttaattt tcctcaaaaa ctacggttga tgtttttaag
    11881 taagcttttg cttcccgatt gatttctttc agatcgcatc cttgctgctt ggcagcgaac
    11941 acgcaaccac aataacattg gcgataaata tcatactctt tgcacatttg caaagagcgt
    12001 tcatagcctt tgtttttctt gaaatcactt ggcaagtatt gtgtcccgta aatcttttgg
    12061 atatccattc cgatctgatt gatcaaagct gcatttttct ttggcgagat ggtcagggca
    12121 cttccaaagt aatcgtatcc tagttcttgc gctttttgcg caacaagatc taatcgcata
    12181 ttgaagcatg cactgcagcg agcaccacct tctttttcgt tttcaagatg gtttgttaaa
    12241 accattttgg tgaattcatt tggacggtat tcatcaacga ttagcgcaac atgatggtca
    12301 gttttctcat taaattggtc tacaaatcgt ttttgttcat gtaatcttcg caaatactca
    12361 cttttaggat gaatattcga atttgaaaaa aataacgtga tctctgcata ttgagacatg
    12421 aattccaatg tgtgcgtact gcaaggagca cagcatgtat gtattaatat cttaggcttt
    12481 acttcttctt tctcccattg catgatcatt tttttcagaa ctgtatcata atttattttt
    12541 tgatttttca ttttttctac gatttgttga gcattaatca atttgttatt cctcctcctt
    12601 cttttttcta ggatttgcct tattataagc catttctatt ttaatcgaaa gctgtattgt
    12661 tatctgtttg ataatttctt tgagtattcc taattcccct tgtatactgg tagaaacgac
    12721 tattgtcagg aggatagtac tgtgcatgtt gatttattct ttcgaaggct gcagcggcga
    12781 ggacagaagt ttgctgccaa tcatttctca tcgatccaaa tcatcgtttt ttattatata
    12841 ttgatgacgg tcttatcact cgttctattt tatatgccga tttttcgcga accagatagc
    12901 catgtttctt ttgtggacat gctgttcatg gcaatcagta cagtgagtgt gactggacta
    12961 tcgacttttg atatcaattc tgtgttcaat gataacggta ttatactgct ggaaatatta
    13021 tttcaagttg gtggtttggg gatcatgatg atttctactg cttttgtcat tttttctaaa
    13081 cgacggatca cactgaaaca gcgacagctg atcatgacgg atatgaatca accgcgtttg
    13141 tctggtatcg tccgactgat tcggatcact tttgctattt taatctggtt tcaattactt
    13201 tttgggacat tcttttctat ttatttttat tacagaggct attttgatcg ttggcgtgat
    13261 gccgtttttt acggttttta tcaggcgata tcggctgtca cgaactctgg gtttgacgtc
    13321 actggggatt ccatcaagcc ctttgcacat gactattttt tcttgatcct gatcatgttt
    13381 ttgatttttg tcggaggaat tggttttcct gtattgatgg agtgccggga atggctatta
    13441 tacaaacgtt ccaatgcgaa gcttccgttt cgtttttcct tgtttaccaa attagctgtg
    13501 ttagcttttg ttatcttgtt tgtttcggga actgtcttga tttatctatt ggaaaaggac
    13561 catttattcc aagattccaa tatgagtgtc aaatggatca attcgatgtt ttattcgatt
    13621 acgactcgta atgctggtct gcagattcac gatttgggtg attttcaaat cacaacactg
    13681 atcatttttt ctttactgat gtttatcgga tgcagtccta gttctgtagg cggcgggatt
    13741 cgaacaacta ctgtagcaat catcggtttg tatctatatt ccttcctcaa aagtgaagat
    13801 aacatcaata tttttggacg gagaatcgat caagatgatg tgcgaaaatc agtcgttgtc
    13861 tttatgcttt ctcttggtat gtgtttcttt tgtatcgttt ttttatctgc tacagaagaa
    13921 caaacgctga tctcgatcat catcgaagta acttctgcgt ttggaacaac tggtttatct
    13981 ttagggatta ccggagactt gtctgttgtg ggcaaaatca cgattgctac actgatgttt
    14041 attgggcgga tcggaatgct atatacattg atgttattcg tgccaaaaga aacacgagat
    14101 ctaggctatg aatatccaag cgagaaaatt attatcggtt aaaatgggaa tataggtaag
    14161 agggaataat aaaatcacag cagcttgaaa caatcatttt gtttcaagct gtttttttgt
    14221 taaatcatgc gattttgtcc gatttcatgc actttcagcc gtaacctttt cttaagaata
    14281 agaaaagcaa tttaaccttt ttttcaacat atggtatgat tagttgaatt tattcgtcag
    14341 caaatcgttt ttggaggtta cataatggga atacgcagtc attttgcgat tgcagttgga
    14401 aaaacgtcgc aatgggcatt aaaaactttt tttaaaggcg gatcaagttt accaggtaaa
    14461 ctagcgctga aagtcgatcc acatatacta gatagtttgg caaaagacta ccaagtcgtc
    14521 gttgtcaccg gaacaaacgg taaaacattg actaccgctt taacagtcaa tattttgcgc
    14581 cagcaattcg atgaagttct aaccaaccct actggtgcga atatggtaca gggaattgta
    14641 tcgactttct tgcaggcaaa accaaaaaaa ggacagaaaa agtttgctgt cctagaaata
    14701 gatgaagcaa gtttgagcaa agtcacagaa tatatgaaac cagaactgtt cttatttaca
    14761 aatattttcc gtgatcaaat ggatcggtat ggagaaatct atacgaccta taaaatgatt
    14821 gtagacggtg ccgcaaagtc accaaatgca acgattatca gtaacggtga ttcaccaatc
    14881 ttcaattcta tcgaaacagt caatcctaga aaatattatg gctttgacca tgaagaagat
    14941 cgtgaacaaa tggctcatta caatacagac ggtgtcttat gtccaaattg tcatcacatc
    15001 ttgcattata aaatgatcac ttatgcaaac ttagggaaat attactgtcc aaattgtgac
    15061 ttcaaacgtc ctgaattgga ttatcgatta actgatatgg tacggatgga caatgtgtca
    15121 gcagatttcg tgatcgatgg tcatgaatac ggaatcgaag ttggcggtat gtataatgtc
    15181 tacaatgctt tagcagctac tgcagtggca gagtacttca atgtcgatcc tgataaaatc
    15241 aaacaaggtt taggatatga cgagaaagta ttcggccgac aagaagtcat caatatcaat
    15301 gggaaaaaat gtacattagt acttgtcaaa aatcctgtag gtatcaatca agtaatcgat
    15361 atgattggtc tagctcctta tccattttct ttagtggctt tattaaatgc caattacgca
    15421 gacggaatcg atgtcagctg gatttgggat ggtaattttg aaaactttgc cgatatggat
    15481 atcccagctg ttatttctgg aggagaccgt catacagata tgactttacg tctaaaagtt
    15541 gccgggattc cagaaaataa attgacagaa acaaaagaat taccagaagt aatcgaaaaa
    15601 atcaaagaat tgccaactga gcatgtctat attttagcta cttacactgc tgtattgcag
    15661 cttcgtaaag aactagctgc tcaaggattt atcaaaggag gaatgaaccg tggctaatta
    15721 tgaactcaat atcgcccacc tttatggaaa tttgatgaac acatatggtg ataacggaaa
    15781 ccttttgatg ttgaaatata tagcagaaaa aatgggggtt tcttgccata cggagatcgt
    15841 tagtatacat gaaccttttg atgctgataa atatgacttg gttttctttg gcggtggtca
    15901 agactttgag caattaatca tttcaaaaga catccagaaa aagaaagatt ctttgatcaa
    15961 ctatatcgaa aatgatggcg tcatgttagc catttgtggt ggctaccaat tgctaggtca
    16021 ctactacatg ggagctaatg gagaaaagat ccaaggaatc agtgcactgg atcactacac
    16081 gttaagtcag gaaaataacc ggtttatcgg tgatatcgtt atccataacg aagaattcaa
    16141 tgaaacttat tatggatttg aaaaccacaa tggtcggact ttcttaggtg aaggtgaacg
    16201 acctttgggg aaagtggtac aaggtcaagg aaataatggt gaagaccaat ctgaaggtgt
    16261 catttaccga aatgtcttcg gttcttattt ccatggacct atccttgcaa gaaatgagaa
    16321 cctggcagct cgtttgatcc gcctagcttt agaacaacgt tacggtaaag aattggatct
    16381 gccctatctt gaaggacaaa cagcctagtt attcgaaaaa ggggaatgtg acaaaaattt
    16441 cgtcacattc ccctttttcg tatcctattt taagcgactt gcttggcata acggttcacg
    16501 ttttgcttga tattcccgat tgttttcgga tcattgattc ctgtgacttc ttgaatcaat
    16561 tgttccaagt tttcatggat cttcatttca tgcaattgac ggctttgttc atcttcagga
    16621 tcatagtaat taaaaataat ccccattgtt gccgtcaaat gtgggcaagt caagttatgt
    16681 tcttgtagtt ctctcactgg acgtgtaaat ctttcttgat aacctaactt acgtaatggt
    16741 gtccgagcga cacgtgtgat cgcatcagag atatttttgt tttggaagcg atgaatgatt
    16801 ttttcaatat acgcatgatg ctgctctgca tcaaaccccc atttggcaat cagcaaactt
    16861 cctgtttcct gcaaaactga ttttaattgt gccacaacta acgcatcttg cattgcttca
    16921 tcaatggtct ggtaacctaa caaggcacct gtataagcga cagtcgcatg accagtattg
    16981 acactaaata gtttgcgttc gatatatggt tctagatcat ctacatataa aacccctttt
    17041 aattggattt ttttattttt tctttggcta tcgtcaatga cccattcttt gaaaggttcc
    17101 acttggacga ataaaggatc ttcatggtgt tgtaatggga cgattcgatc gactgctgca
    17161 tttggaaagc cgatatattt gtcagtaaat gatgtttccg gaagatattt atacacttct
    17221 ttttctagaa atgctgaacc gccaatcata ttttcacagg caatgatatc taaagatgtt
    17281 gtgcttcctt ccttttctct ttcttggata ccttttgcaa tcaactcagc aataaaaggt
    17341 aagatattcg ggccaatggc ggtagtaact aaatctgctt cagcgatttc ttggattact
    17401 ttttctggat ttgtttgatt gttgattcct ttgacatttt ttacagtgat tttttctttt
    17461 gattcatctg ctaattcgat ttcgtattct tttctttcat ttaaagcatc aatgatcgtg
    17521 tgattcacat ctacgaaagt aatctcaaat ccattagctg ccaaaacctc tccaataaat
    17581 cctcgaccga tatttcctgc accaaaatgt actgctctca tttttctctc tccttaagca
    17641 attgctagat tttccgttac ttcttctttg ctgagcgcgt ctgctagctg gacaacattg
    17701 tccatatcac tgcaataaag tgcgatttgc tgtacaagtt gcaaatgctc atctccgact
    17761 cctgcaatac cgaacaaaac agtcgcaatc ttttcttctt cttcggtacc aaaatctaca
    17821 ccatctggta cttgaacgac acagatgccg gattttttca catatttttt tgcttcatct
    17881 gttccatgag gaatcgcgat aaaattcccc atataaacag ataacatttt gtctctatct
    17941 accatggcat cgatatattc ctcatcaacg caccctgctt ctacaagcat ctgaccgcaa
    18001 tagcggatcg cttcttcttt ggttttaaaa gatcgattga gctcgatcat gtcgaatggc
    18061 aaactttgca tgctgttttc ctcccttaag ctgtctgttt caaatattgt gcaatcgatt
    18121 cgtatttttg cggattgacc atgctgttga ctgataaatg aatggattga ggtgcagcca
    18181 tctgtacttt ttccgctaaa ctttctttgg ctacgatcag tgaatcaggt tcatctttta
    18241 aatcttggat attgactttt cttaacggga tagttaaatg gttcttgtct aatatatctt
    18301 tgagcacggt catccccatt gttgctgatc cggaacgatc atcgtggaca agtagaatct
    18361 ctttgatttg tttgatgttt gctgaaagaa actcttctgc tggctcattc tcaatttcag
    18421 aaactgatgt tttctgcttg ctgcttaatt cttgaatgat ttcatcatat cgaggtgaat
    18481 tcaaaaagtt ttctactgag acaaaacgcg cgctcggtgc tttttgtttt gctctttctt
    18541 gtagttcgac ctgagtaacg atcaatgtat tcgagtcatc tgtcagttgg ctgattgctt
    18601 gattggtcac cgtttgaggc aagtctgctt cctgtacttt ttttctcaag atcgaagcgc
    18661 ccatcgcact tgatcccatt cctgcatcac aagcaaaaat gattttttgt acgccagaga
    18721 atgtagctcc ttgaggaata ccttgggcac ctttggaagc ttgcttagct gcctttgtct
    18781 cttgaacaga tttttcgaag tcttcatttg tatctttcgc atccgctttc aaaattactg
    18841 cagaaatacc aaacgaaaca gctgcggcaa caatcacgcc caaaataaca ggaaggtaag
    18901 aattgaacgg tgtcatcgct agaatcgcaa tgatcgaccc tggagaagct ggcgctctta
    18961 gtccagcatc catcaattgg aaaatgaaac ttcctgacat tcctccgcct ataactgata
    19021 agaataataa cggtttcatc ataacgtaag gaaaatagat ttcatggatc ccgcctaaaa
    19081 agtgaatgat catggctcct ggtgctgaag attttgctgc tcctttgcca aacaaagtaa
    19141 aagcaagtaa tacgccaagt cctggaccag gatttgcttc aagcaagaat agaatagatt
    19201 ttccagcctc tgctacttgt tcagttccca atggtgtcaa aatcccatga ttgattgcat
    19261 tattcaaaaa taagattttt gctggttcaa taaaaatact tgtcaatgga atcaaatggg
    19321 cagataaaat gatctctaca ccttttgcca ttgcttgagt caatgcatcg acaactggac
    19381 caatcgcaga aaaacctaat aacgctaatc caaaaccgat aagacctgct gaaaaattat
    19441 tgactagcat ttcaaaaccg gatttgatcc gtttttggaa tagctgatca aatttcttga
    19501 tggcatatcc accaagaggt cccatgatca tcgcgcctag catcattgga atatctgttc
    19561 cgacaatcac acccatcgtt gcaattgccc caacaactga tccgcggtca ccagcaatta
    19621 cttttccgcc agtataaccg atcaataaag gaatcagata agttagcatt gggcttacca
    19681 tggtagcgaa agcctcattt ggtaaataac catctgggat aaatagagcc gttaatacac
    19741 cccaggcaat cagtgctcca atatttggca tgaccatgct tgataaagta ctgcctaatt
    19801 tttgtaccct cgcttttagc gaaatgttgt ttttgggaat cgtccgttcc attttgtctc
    19861 tccctctctc cttttgatac tctaattgta tccgcttact aaaaagagaa atagtcgtac
    19921 tttggcacga aactttgtgc caaaggtttg agtatgtcag caaaataaaa agatttcggt
    19981 atcagaaaac ttcaatttta aataaaactg ttattttaat ttttttataa tatgcaaaca
    20041 ataacttttg ttagcattta atttagatgt tttccattct attatgttat taaattttat
    20101 attggaggcc tatctatgac attactgatc aaaaacatga acatgaaacg tggtaaaaaa
    20161 cgtgtgattc gacaattgaa tcttttagta gaatcaggag aagtagctgc tttgattgcc
    20221 ccgaacggat ttggcaaaac aactttgctg aatggcattg cccaactgct gccaacaact
    20281 tatgatcaat tgtcccttca caacatctct cctactgctt atacagatta tcgccggtct
    20341 ttcttttttc tagaatcaac gaatcagtta tccgactatc tatcggctgt tgattatttg
    20401 actttgatca aatactattg gcagagcaaa gtagaatgcc aaactgtttt ccaacgattg
    20461 aagatgactg agtaccttac tttgccgatt ggaaaaatgt ctctaggaat gaaacaacat
    20521 gtcttgcttg ctgcttactt gatcagtgat tcatctgtca tgttattcga tgaaccgttg
    20581 aacggattag acccaggaag tattgatatc ttgaatagcc tcatcagaca atggcagaaa
    20641 gaggggaaga cgattcttct ttcttcccac gctatcgcga atatccaatc gatttgttct
    20701 aaagcatgtt ttctaaaaaa tggagaaatc gtcacagaat cctcaaatat ggaagagatt
    20761 ttagctattt atcgtaaatt atatgaggtg taactatgtc tttacttaca tttgaacgca
    20821 atctcttact aaaaaacaaa gtaaactttt tatttcctct attgttggtc gtgctctttg
    20881 cattcccact tttttttgat cataaacttg cttatacaga attcgatgaa ctgaatcata
    20941 actatgaaga gatgcaacgt ttgattgaaa cgttgaaaga agatgagaat gagaaagaat
    21001 ttgtggaaag tttagagaaa agcaacaaac tgatcgaagc aatcttacat gcaaaaaaca
    21061 cagggaatgt ccagcaaatg gtcgaagcaa cttatcatta tgaaaaagat atactggatc
    21121 gactgatttc tggtcaaaga caaggaattc caatcattga acaacaaaaa cgtgtcgagt
    21181 tgctccgtta tatgaaagaa catcagatcc aaagatattc tatttttgac ttgccagcgc
    21241 atctctcttt agcaaattac tacgaaaata ttttttcagg aatgatttct agttttttga
    21301 ttttatgtat aaccgccttg tttctttctt ccatcatctc ttatgaaaaa agaaagcagg
    21361 taatctctct agtcaatcta ttaccggata gtatggtaaa gaaacatagc atccgtttta
    21421 ccatttatta tggtgcagct atgctaagtc tcgtgatgcc atttttgatc gtctctatac
    21481 tagtaataat caaaaatggt ttgggagact tccgttatcc tgtaggaacg atcattggtc
    21541 aggagatacg aatcctgccg atgtatgagt atcttttcca gagctttctg ttcctgttgt
    21601 tgtgggtgct ctttttaagt acaatcagtt ttcttctatc tgctttattt gaacattctc
    21661 tagtcaatct tttaggtaca cttctttgtc ttttcttagc agagtatcgc ttgttttctt
    21721 cgattggatg gatagaatca atcagtcatt acttgccaac ttcttatgtt gattttcaaa
    21781 atgtgatcat aggaggagat attttctcgc cactagcttc agaacaggtt actttcatga
    21841 atggtatctt gacacttggt atatggagta tcgttttatt attcataggt atgggcacta
    21901 tctatataaa aaagagctat taaaacacaa aaataatagg ggcggtgaca aaaaccttgt
    21961 caccgtcccc tatttcaaat aaacggtttt ctagaataaa tagttttcaa aagcttgcat
    22021 ccgcagtaat aagaacctcc caacaaaaat agctcctctt atttttatct tggttgaagt
    22081 tattacttga tgcaaggcag ctttttttac tgtgaacatt ggttatatat gtccatgttc
    22141 aaaaatcatt tggacttctt caatggtttc cgcatcactg agcgccatta cttggtcgat
    22201 gtctgaacag aaaaaggcta aatcttggag caccgctaac tgatgttctt tttttaaagc
    22261 cactacaaat aagagtgtcg cgatttttgg ttcctctttt gttccaaagt ccacaccatc
    22321 aggcacttgg actaaataga ttccttcttc tactacttct tcatctttgt cagtcccatg
    22381 tggcaaggct acgaaattcc cgatatagac actcgctttt tcttgtcttt cgatcattga
    22441 ttggatatat gccggtttga ttcccccttg ttgggtcatc cactggccga tctcggtcaa
    22501 cgcttctttc caattttgat aggattgatc gagcaatatt ttttctgctg gtagctgtat
    22561 catcttttca ccttcatttc tttatttctt caatcaggat tttcgctaac aactgataaa
    22621 cgatttcttt atttcctgag cggaagatct cggtgttcaa gtcattcatg atgattgctc
    22681 cactgatctt tcctaaaaga atcccgtctt tttcttggat tggcgaaggc gccagcataa
    22741 ctaacatacg ggacaaatcg actggctgtc tatccatccc ttcaatggtc aacggatgtt
    22801 tcaaattgaa aatacaaaag atcggtttag aaatcaatga gctggatgta tggaataacc
    22861 ccatatttgt atgaggaata ccgataggtg cttgttcata tcgcttaagc agccggcgat
    22921 gaaccacctc ttcatccgtt actagtcctt caggaaaata aggaaataag ctgcgaatcg
    22981 tttcggaaat cgtctcagga ttatccagtt cttggataaa aaaacgaccg agtaattgat
    23041 tgatttgatc catgaaatgc attgtttcct cataagtatc ttctatcatc cactcgttat
    23101 tgctgttata cgtaattttt tgcctttttt tcttctcaat atgcgaaaaa gcttctttta
    23161 gctgcttgac ttcatcttct agcagtagcg gggagactaa aatatatttt ccaccgtatc
    23221 ctggtaaaat agatgtagaa ataacaacat catattcttc ttgtaaatcg atttttttta
    23281 aatcagcaat ccgatagaaa tgaatttgtt ggatgaaggg aaaatacctt ttcaatcgta
    23341 gttccaacat gctagtagaa gctaatccac ttggcgaaaa tcctgcaata ctgatattgc
    23401 tttctttcgg acttcgttcc aatgaattcg caaaatgaag gaccatataa gcgatctctt
    23461 cttcggataa ttttttttct tgaaaaatcc gctctactgc tttacggatt gcattcgcaa
    23521 cttcctcgta ttgaaccatg attttttcta aaatcggatt ggtcagtgtc tcttcttgta
    23581 aaacggctct tgaaaacaca ccagataaat gagtcaacag cattttgtac aaagtttcat
    23641 cttcataaaa atggacattc gtctcttcac ttacttcttg aatcagttgt tttactaaat
    23701 aagcaagctg tgtatcgaac gtctcttcaa agaaattctg atcaaatgag ttcgtgaagt
    23761 catttaataa tcctgcaaaa aagacgatct catttactgg ataaagttgt tttgtctcag
    23821 aagcgatccg tgaaaacaat cgtttagctg tctgtagtaa ttctttgttt gccagactat
    23881 tatatgtctc atttgtcagt aaatgatttt tcgtcacacg ctccatcgtc aatgtcagca
    23941 tcaaaaccaa atgttcaagc tttctatcac ttagattggt ctttttttgg atcaattctt
    24001 ccccgattac tttgcgtgca agagatagat gttgcggatc aacgaatcga aaaaaataat
    24061 tcagtgatgc atcgaaagaa atactatgaa aaagttcata ttcattgatt tctaattcta
    24121 atacattcgc tgtcagaagc cggcggtatt tttccggtcc ttcgatctcg tatccctggt
    24181 tccgaatgat ttccaaaggc agttggcgaa tactttcttc caactgtttg atatctgcat
    24241 aaaaagtggt attactgatg cgatatgatt ccatgaaatg agcaagactc aacggctctg
    24301 tcgtcagtaa caactcggct aaaatcgcgt gttgtcgttt agcagtggat aagtcattac
    24361 ttttcccatc tgtattttct tttaattgct ctaatacttc ttctgatgcc tcaatgacaa
    24421 atgagcctct agctgctttt ttcaaagaag catttaccga tttcaatgag acttcaagat
    24481 tatctaattc gcggtaaata gtccgtttac tgaccttcaa taaattcatc attttttgga
    24541 tagatacagg tgacgggcta ctgagaaatt ctgctaataa ttgtttttct ctgggcgata
    24601 aatacatctt atatttctcc cttcacgagt ttttcgtatt cattcgtagc tacccaatgt
    24661 tcaactagaa ctagccgagg gatcttttct tctagctgtt cagttacagc tagttctctt
    24721 tgtgcgatat aaatcgtttc ttcttggatt ttgatttttt ctattgattc ttttgagata
    24781 acaaactgtt ttttcgcttt ttttgctaat tgacttaaaa tcgtcatgcc catagtttgt
    24841 gagccgcgtc tgttgttttt atacaaaaat acaatattag caggcgacgt tttttgttct
    24901 gtgatatctg ctatctcttt tttttcttcc cgctttggaa attggatcgc ttcttttatt
    24961 cgtgacagca agatatctgt ttccaaaaag tgatccagta catagatttg actttgaggt
    25021 actttttctc ttgcaagacc cgctagttca tgttgcacga taaccagctg atttacttgg
    25081 ttattcaatt catagataga gctgtatccg acagaaagat cgattttttc tgctgctaat
    25141 ttctttttca gcagggcggc tcccatagca cttgatccca tacctgagtc gcaagcaaaa
    25201 atgacggaat gaatgataga tggttccaag atttctcccc tttctttgac cgttttgttc
    25261 tctttttctt tatttggtaa tttcaatagt atcgctgcac aaatgaaggt cacaaatgta
    25321 ctgattgcta tccctatact taccccagct agttgtggat aaggcgcatt ggcaaaaatc
    25381 acgagtagtg acccaggcga tacaggtgca gctaatcccg tatctaacca ttgaaatatc
    25441 aacgtgccgc ttgccccacc agccattaca gctaaaaata gatacggtcg cagcaagaca
    25501 aatggaaaat agatttcgtg aatcccgcca atcgcatgga tcatcaatgc tccactcgca
    25561 tttacttttt cctgtttttc tgcaaaacaa aggatagaaa gtaagatccc taaaccaggt
    25621 ccaggatttg cctccatcaa aaataaggaa gagtacccga ctgtttgtga gaattcgatt
    25681 cctaaaggtg tcaatacacc atgattgatt gcattattga aaaatagtac ttttagaggt
    25741 tctaagatca gactgacaag tggtaacaga ttcattgtga ctagccaaga cacaacggag
    25801 gcgagcaaca agtgaacact atttaaaact ggttcaataa aaacgattcc aattatacaa
    25861 ataacgctac cagtcagtcc tactagtagg ttgctgacaa gcatttcata tccagccttg
    25921 aatttcacat ggaatctaga aacaaattgt tcatatacga tgccggcaag cggcccgata
    25981 atcatcgctc ctacgatttg tggagcattg gaacgagcga tcaaccctaa agaagcaatt
    26041 gcaccggcga ctgccccttt ttgtccccct accgatttcc cgcctgaata actgatcaag
    26101 atgggtaaaa gcagctggat catccaatgg catacctctt ctaatgtggt gtgcaaatcg
    26161 ccagacacac cttttgttgc taatgacaag attccccaag cgataaacag acttagattt
    26221 ggcatgatca tttcatttaa gtgatgtcca gcttttctca gttgtttcat agacacgcga
    26281 ctgctgttgt ttgctttcca tttgtattcc atctttttca ccagacttct atttattgat
    26341 ggacggattt tatgtagaaa aagcagcaaa cgaaaaaccg tctgctgctt tcagctatct
    26401 gggaactatt tgcctggttc agacgcaatg attgctagtt caccttggac cgcccactca
    26461 gaaacgccat ttggaataat acagcttgtt cctttttcta atggatatgt ttttccgtca
    26521 acgacaaggt ctcctgctcc ttcgattacc gtcatcaatg tataaggggc ttgttttttg
    26581 aaagatgtga ttcctttaat atcccactca tatacattga aaaactcagt ttctaaataa
    26641 gtgacgatcg atgaattccc ttttcggact tctttgattt gtaattctgg tgttttcgca
    26701 gggacagttg ttacatcgat cgattgttgg atatgcagct ctcttgtttg tccttgcgca
    26761 tcttttctgt cgtagtcata cacgcggtaa gtcgtgtcag aactttgctg tgtttctaaa
    26821 atcatgattc ctttaccaat agcatggatc gttccgcttg gtacataaaa gaaatcacct
    26881 tttttaactg gtactttctt caaaaggtca tcccagcgcc catctttgat catctcagct
    26941 agttcttctc ttgtctttgc atgatgacca tagatgattt ctgcaccagg ttcagcatcg
    27001 atgatatacc agcattcagt ttttcctaat tcaccttcat gtttcatccc ataggcatca
    27061 tctggatgta cttgtacaga tagatcatct tctgcgtcta agatcttgat caataatgga
    27121 aacactggtt ctgaaggatg gccaaacaat tccttgtgtt ctgaccatag ttcatctaat
    27181 cttttccctt tgaattcgcc attttctact acactgacac catgaggatg cgcactgatc
    27241 gcccaatctt ctccgatttt atcgttaggg atatcaaaac caaagactga acgtaaacga
    27301 cttcctcccc aaattttttc ttgaaaaact ggttttaaaa acataggttc ctgcataatt
    27361 caaactcctc tcatttttca tagctattat agcattattt cggtaatacc gctttctctt
    27421 tcaaaaagtt ttcaacaatt tcatctcgac atatgagata ctccgcacga tgatcgacag
    27481 gatgtacgcg atttgacaaa aatatcaacg cttcttgtgt ttttatatca atcaagaaaa
    27541 acgtacctgt ataaccagta tgaaaaagaa tcgcatgacc gtcttctaca tcctttttta
    27601 agtcccatcc taaagaacga tagccatttt ttttcggtgt ttgatcttgc agtaaagaat
    27661 caatcgtctc ttcttctaaa aaacgaccat tacggatttt tcccttgttc aaatacattt
    27721 ggacaaaagt aatgctgtct ttcaaatcaa taaagagacc cgcgttacct gcatgttcag
    27781 ccaaaactct tgctttcgga tcatgagttt gtccacgcaa aacctcaccc gaaggcagtt
    27841 gttctgtggg aacgattttt ttggaaggaa aaggaagaaa taagctgttc atcattccta
    27901 atggttccaa tacttgttct tgaaagattt ccactgcagt ttcttggtaa atttcctcta
    27961 gcatcaaccc aagaagaatc gtaccggcat ctgtatattt tacttctttt cctagttgct
    28021 cacctggttc tagcttcaaa taagcggcga tcaattcttc ctgagatagc ttatctctgt
    28081 ttggtatcca tgttttgata tcagaagtat gtgtcagtaa atgacgcaaa gtgattgtct
    28141 gattttcaaa ttcaggaaga tatttatgaa gcggttgatc gagttctatc tttccatctt
    28201 ctaatagacg taaaactaat gaagtggttg caacgacttt cgtcagtgag gctgcatcaa
    28261 acaacagatc ttctgtcatc agctgctttt caggaacgac ttgggcaaag ccggctgtaa
    28321 atgtttctac ttgattttct tttatgaagg cgactgctac acctggataa aatccttctt
    28381 ccatcttttt caaaataagc tgtttcgtgt gtggatacat ctttttctcc tgcttttcta
    28441 tcagattctt acagtccaag cttaaagctt aatctatgag gaatcaacct tttttacaca
    28501 aaaaaactgc gcgaaaaacc gcgcagtttc ccccggtata ctattttctc tttctatctc
    28561 tagaataagc gatagtatga ccgttcataa aatgacgaga catgcgacaa tcgttaagaa
    28621 agaaaaagtt aagcgtcgca tcgaatgtca gttttattct tttcttttgg ctcactgacg
    28681 aaccaccatt cgattcctat agcatcatag attgtgagta ctgtgtgctt tttgtctaag
    28741 aagaaatcaa ctgatagttg ctgcaagtga gcaatcagtc cttccatgct ttcagtatcg
    28801 atcatgaatt tcagaaaatc aagtccaatc acttcatctg taggcaaatc aattgcgcct
    28861 ccgctagctt ctgtcaaacc aacatggaaa tctccttcat tcaatacatg gatgccctct
    28921 tcttcattat gtaccttcaa accaagtaca tgattaagaa attggcgttc ttttgttaag
    28981 ttagtaacat ttaaatggat tttttcaaaa taagaacctg ttgatagttt actgaatttt
    29041 tcttcagatt ttcttaataa tgcttcttga tccattgttt ctggaatttc tctttggtcg
    29101 ttcttattct cttcatggta aatttccaat tcattctctt ctgggtctac gagcaaaatc
    29161 cccatacgtc catgatcttc taaagcttct ttgattggat aattcatttt acggatacga
    29221 caaaggatat ctgccatttc ttctgtgcta gggataatca atgaaaaacg gttcagcttt
    29281 ttgatcaccc ccatgtgatc attcgctcta ggactttctt ccagcagcaa catttcactt
    29341 ccagcttctt ctgttcctaa aatggctaat tcgttttctt ctcttttcaa atcaaagcca
    29401 acaacgttct tgtaaaaatc aatcatttta tctcggtctt tcacccgaat ggctacactt
    29461 tttaaatagg tggcagcccc caattgaaaa tctttcatat ttcttgctcc cctctacgtc
    29521 aagctttatt ttaacgatgg attcttcttc tttcaagcaa taactttgat tttgttaaaa
    29581 aaaatgtgag aaaattcgca ttttgcttgt tttttgtaaa ttaatgagta aaaactctta
    29641 taaaaagagg ttgtgaaaaa gctgttctgc atcaagtaat aagaacagcc aaacaaaaat
    29701 agctcctcct atttttcgtt tggttgggct tattaccgca gatgcaagct tttgaacacc
    29761 gtttaaggaa gaggttgtga aaaagtcgct tagtttcgag tagtaagaaa aattttgtga
    29821 aaatggcttt ctacattttt gcataaattt ggcttaatac cgagaaactg gcttttgaac
    29881 accgtttacc aagaggttgt gagcctgccg tttaactccg agtcacaagg agcaatttca
    29941 aaaaacagct tctcaggttt gttgactatt gtgacttgtg accgaggagt tggcaggaga
    30001 acaccgttta aggaagaggt tgtaaaatca gcggtttgac ctgagtatta gagatacaaa
    30061 acggaaaaat agcttctcat attttgccgt tttttgagct aataccgaag gacagctgat
    30121 tgaacaccgt ttattcggaa aaagggactg tgacacgact tttgtcacag cccctaaaaa
    30181 aaataataaa tttgtactat tctatgaagc ttcttcttca gaaaactgac tgttataaag
    30241 atctgcatag aatccgtctt tttccatcaa tgtatcgtgg tttcctgttt ctacgatcga
    30301 accatgattc atcacaataa tgttgtctgc atcgcgtatt gtagacaagc ggtgagcaac
    30361 aacgaaactt gtccggtttt ccaataggcg gttcatcgct ttttgaatca gtatttctgt
    30421 tcgtgtatct acactagatg ttgcttcatc aaggatcagg acatctggat ttgctaagaa
    30481 tgcccgcgca attgtgatta gctgacgctg cccttgcgaa atattgcttg catcctcgtt
    30541 taatacagtg ttatatcctt ctggcaattt acgaacaaaa tcgtctacgt gagcagcttt
    30601 agcagcctca atgatttgtt cgtctgttgc atcgtcgttc ccatatttga tattgtcata
    30661 gatacttcct gtaaacagcc aagtatcttg cagtaccatt gaaaactgtg cgcgcaattc
    30721 atcacgtgaa agatcacggg tatctacacc atcgtaacga atactacctc cagaaacatc
    30781 gtagaaccgt tcaagaaggt tgatcagtgt cgtcttccct gcaccagtcg gacctacaat
    30841 agcgaccatc tcaccaggtt tgacatccaa gttaaaatca gtcattagca agttatctgg
    30901 tgtgtagccg aattgaacat tttcaaaacg aactttgtat ggtgtatctt tttcttctgg
    30961 aaggccagat ttttcattgg acatctcttc ttcatccaac acttcaaata cccgttcggc
    31021 tgaagcaact gttgattgga tagtgttcat caaacttgca agttgagtga ttggttgaga
    31081 gaactgtgtt gtatattgca agaacgcttg aacatcccct aatgtcatgt tcccatttgc
    31141 aacttttact ccgcctagta cagcgacgaa cacatagcct agatttttaa cgaaattcat
    31201 caacggcatg atgattgccg aaataaattg agctttccaa ccagcggcat acagattctg
    31261 attttctttt tcaaatactt cctggtcttt ttcagtatga ttgaatgttt taacaatggt
    31321 atgaccacca tatgtttctt ctacttggtt attcaataaa cctaaacttt tttgctgatc
    31381 tgcgaaaaac acttgtgatt tcggcgctac gatcatcacg atgatcaagc ttaaagggat
    31441 catcaataac gcgactaacg tcaattgcca gctgattgaa agcatcatca cgatgacccc
    31501 aatcaatgtg actgcactag ttacaaattg agtcaaattt tgctgcagcg tgcttgcgat
    31561 gttatccata tcattgattg ctcgagacat gatatcccca tttgtatgtg tatcataata
    31621 ggaaatcggt acacgattca ttttggcttc caaatcatga cgtaattcat aaactgttcg
    31681 ttgggaaaca cgtgtcataa taaattgctg taaaaagttg aatacagcag aaatcaaata
    31741 catcacgatt actgtcgcaa tgatctgcgc gattttatca aagtctatcg gaaattttgt
    31801 tacttgttgg ccttgttgca tctgttgagc gccagccatt acacctttga atatttctgt
    31861 ggttgctttc cccaagacct ttggtgtttg gatttggaag atcacggctg ctattgctag
    31921 tacaaataca gcgatgatcg caaccatacg tttagacata tagccaaaca atcggcgtac
    31981 cgttccccag aaatttttag gcttttcacc ttttacaccg agatttctgc caggtccacc
    32041 atgtcctgga ccgcctgctg gtccatgaga gggacgtgtg tttgttttac tcatgctaag
    32101 tcctcctctc ttagttgtga atgaacgatt tcttgataag tttcgtttgt tgctaatagt
    32161 tcttcatgtg ttcctttgcc aaccagtttt ccttcatcta gcacaaggat catatctgca
    32221 tccattactg tacttactcg ttgagcaact aaaacggtga tactttcttt catattcgtt
    32281 ttcaatgctt ggcgaagatt ggcatccgtt ttgaaatcca acgctgagaa ggaatcatca
    32341 aacacataga tatctgctgt tttgactaat gcccgtgcaa ttgccagacg ctgtctttgt
    32401 ccaccagaga agtttcctcc tccttgttcc acatggctat ctaagccatc ttccatctct
    32461 gaaacaaaat ctttagcttg tgcgatttct aatgctttcc aaatcatttc atctgttgca
    32521 tccggcttac catactgcat attgtctcgg atcgttccag agaacagaac tgctttttga
    32581 ggaacaaagc ccattaattc tcgcaaatta tattgagtca tgtcactaat gtccgttcca
    32641 tttatttgga tcgaaccaga ttcgatgtca tataatcgag ggatcaaatt gactaaggtc
    32701 gttttacctg aaccagtccc ccctatgatt gcgacgatct cgcccgactt ggcttggaaa
    32761 tcaatatctt ccaatgcaag tttttctgct ccatgatatc gataattgac atgattgaag
    32821 gccaatgttg cgttttttcc agcaaaagaa actgttttag gattttcagg gtcatggata
    32881 ccgatttttt catccaacac ttcattaata cggtcagctg aagcttgtgc acgaggcacc
    32941 atgataaaaa tagcggaaag catcataaag ctgatcaaga tctgcatcgc ataagtcata
    33001 aatgcaatca gattaccgac ttccaaagtc atatctgcta tgtagtgtcc accaaaccaa
    33061 gtgattgcta cgtttgtacc gctcatgatc aatgtcatca ttggcatcat caatgcgacg
    33121 atcgtattaa ctttgattgc cgtatttgta taatctttat tcgctaaatc aaaacgattt
    33181 tcttcaaagc gtgtcttatc gaatgctcga atcactctga cccctgtcaa tccttctcga
    33241 aataccaggt ttaaacggtc agttttcttt tgcatacttt tgaataatgg aacagcaaag
    33301 tacatgattc cgccgatcaa aataatcatt actgggatga caactaagaa gatttttgtc
    33361 aactgatgat ctttttgata agcaagtatg ctcgcgccaa tcaacgtgat tggtgcattg
    33421 atcatcatac gtaaaaacat ttgtgtaacc atttggattt gagtcacatc attggtcgtt
    33481 cttgtgatca aagaggccgt tccatattta tcaaacgcat cttttgtcag actctctgat
    33541 tttttataga catccgtacg caattgctta cccagtttct gtgattcacg agtagcaaaa
    33601 aatgtatttg cgatagcagc taagatactg atcaaagaaa agccgatcat cacaaaacct
    33661 gtatgccaaa tataatcgac atctccttgc gctacccctt tatcaataat attggaggta
    33721 agtgtcggca aatataaatc tgccagtact tggatcacca tgaaaactgc agctgcaatt
    33781 gctgaagtca aggagatccg ttttacgatt tttagcatct agttcccttc tttctattta
    33841 tatactccgt ttttcagcca agaaagtttc aaattgaatt cactgattgc ttgctctact
    33901 gagtaatctt cagatgttga aaataagcga tacgaatgcg caattgttgt aaatacagta
    33961 ttcatcacta attggatcag taattccaat tcatgatcat tttttacttt cagcttcgtc
    34021 cgatcgatca attcaagcaa ctcttttgca tcttgtttgt gttctttatg tggatcgtga
    34081 ggcgtttttt ggcgactcgt atgaggagcg acttttcttg aggcacggta atccatgttc
    34141 ataaacaagt tacgataaaa agaagcattc tcgccttcca aaatctcata aatcatttta
    34201 gaaaaatagg attcgaatcc tttgaataga tcaccatcgg attctttcat tagttgtaac
    34261 atatctttag agctgtcacg tctcattgtc tcaaagtaat aaaaataaag atcctcttta
    34321 tcttcaaaat actgatagaa actgcctcta gggatatccg ctaatttgac gatattagca
    34381 atcgaagctt cttttaaggg tactctagaa aactcgattc tcgcagcctc gattagtctt
    34441 ttttgttttt ctttaggcaa atggaaaaat gtctgtgtcg gcatatggat tctcctttct
    34501 ttcaagatga cacattgtca tatgacgcta tgtcattata acacctccta cctgttttgc
    34561 aagacatttg catagttttt ttcattatca tccgatacat taaaaaagtg ataagaggaa
    34621 tccttccatt tgaattaatg agagttgccc attaaaatag ccacatacta aacaaaggat
    34681 gagataaatg gcccaatcaa ttagtgtcca agacttcaat aaattgcctc tcgatgctga
    34741 cagcatcgtt ttagatacaa gaaatacctt agattacaca aaaaaccact taagccactc
    34801 gctttctatc cccgctgcta tcttgccaag acaattaaaa aatctagatc caaagaaact
    34861 ttattatatt ctaagctata caggcaggcg atcagaagca ttagccgatc aacttactgc
    34921 taatggtttt cgtgctgttt atatcattgg tgggatggaa gctcttcagt cgagtgttgc
    34981 ttgaatattg cttgttagat tcaaaagagg ctgtgacaaa aaatcgtgtc acagcctctt
    35041 ttataccctg tcttttttta gttatttatt cttcaacctt atcaatatga cttgatagcc
    35101 ttttcttttt cgagtaaact gattttgtta gtcatagtta taacaatcat agcaataaaa
    35161 ggagaaggta agcatatggc atctgtttta gtagtcaaag gtcatcccct aactgcagaa
    35221 gaatcgcgca cagttaaggc attgacgtct tttttaacaa gttataaaga aaatcatcca
    35281 gatgatgaag tgactgtttt ggaactttat cgcgatgata ttccggaaat cgatgaagaa
    35341 ttattatcag gttgggaagc attacgtgct ggtgcagaat tcacttcctt atcagaaagt
    35401 caacaacaaa aaattgctcg tttcaatgaa ttgactgatc agttccttgc tgctgataaa
    35461 atcgttatcg caaacgcctt atggaatcta aatatcccaa caaaattaaa agcttggttc
    35521 gataccgtca atgtagctgg aaaaacgttc cgctatacag aaaacgggcc agaaggatta
    35581 gtgacaggta aaaaagcact gcacatccaa tctaatggcg gtgtatacaa cggacaagac
    35641 tttgcctctc aatatgtaaa aggtatttta aactttgtag gcatcgatca agtagatcag
    35701 ctattcatcg aaggaatcga ttatgaccct gaccgtgcag atgagttgat gcaaaatgca
    35761 ttagataaag ctgctgcttt aggtaaatca ttttaaaaat tgagataaat gcatacaaaa
    35821 gagacagagg aaaaaatctt caaagcagca tttgtgcctt ggatattttc ccatctgtct
    35881 ctttttgtta ttctttctgc tttttcaacg gattttcttc ataaagagaa gagattgttg
    35941 tgtaccattc aaaaacatga gtaatcacta ctttagctgc tgcataaaca ggaatcccca
    36001 agatcacacc gatgattccg aacaatttcc cagaagtcag cagaactaat aaaatcgtta
    36061 ctggatggat tgctaactga ttccctagta ctaatggaga gatcaagcgt ccttctattg
    36121 tctgctctac tgcaaaaacg atcagtactt tgatgatcat gaatggacct gctacaatgg
    36181 ctaaaaagat agccggaatc atcgctaaaa atgatcctaa gtaagggatc aggtttaaaa
    36241 aacccgcaat aatccccaac gtgacagcat actctaaacc aatgaccgca aaaccaatca
    36301 taaacatgat tgccacagca aaagccacag ttagttgtcc tcttatataa gaagaaactt
    36361 gactattcat ttcactcagc acattcaacg tcggtttttg catgcgtgtc ggtaaaaatt
    36421 tgacaaaata aggtgctaat tgtttaccgt ctttcaatag ataaaataag ataaacggca
    36481 tcgtcagcag agccactaaa atagtcgcta ccgcgccgac aaaacttcca agactctgaa
    36541 ctgtactctt agatatatct tggatcatat cgcctgccga attcatgaac ttatcacctg
    36601 ctgtttccaa ctgttcgcga aattggttga acagcgggtc ccgcaaaatc tcggttaatt
    36661 tattatcaat cgtatctaca tattgaggga agttactgat gaagctcact gtttgttcct
    36721 gaatcttagg gatgatcact acgctccccc atgcgattag tgcaacaacc aaaacaaaca
    36781 gcccaatgat actgtaaagc cttggtactt ttcgcttttc aaagtaatca actaccggat
    36841 tcatcaagta atacaagatt cctgctaaaa tgataggcag acctacgatt gctaaaaatt
    36901 gccagattgg tgcaaacaaa tatgaaactt ttgtaaataa aaagacgatc agcaatatta
    36961 acaatacaac aagtaaggct gtgactactt gattatttaa aaaccagcgc caaaaccacg
    37021 ataccggtct gttcttctta ttttttcctt ccataattcc tcctgttagt tataaaaaat
    37081 ttggcttccc aaaccaatag caggcagtct atgcggatga agatagattc catttgcaat
    37141 ttgtccttct tctggacaag catcaaaaat caatgatacg tgagctactg tctttaaatt
    37201 cccattggcc atcggaccga cataatcgat cgtatatacc tgatcatcaa aagcaattgt
    37261 tccgcctgct ttcaaagaaa aaggttcgtc ttttgatact tcttgaatca ccgaatattt
    37321 tcgtaaggca gctgttgctg ttttatcgaa caaaatcagc atgggttctt ccttgtttat
    37381 tgcttctgtc ccaatttctg taatctttgc ctgaatcatt gcactctctc ctttaattgc
    37441 tttcaaaaac agtataacac attctcattc tttagccgtg tggaataatt atgtgataga
    37501 ataaaaacga aaactatttc attacaatta ggaggaatca gctcatatga tcgaaaagat
    37561 aattttagga acctatacgc gtcgcgaaag taaaggaatc tatacgatcg accttgatac
    37621 agaaaaagaa gaattagcca acctttcatt agctgttgaa gaaaatagcc ctacctatgt
    37681 agcaaaaagt aaagctggca atctttacac agttactagt gtggatggat taggtggtgc
    37741 cggtgcgtac gataaggatt ttcatttttt gaatgcggta acagagtctg gcgctccttt
    37801 gtgttatgta gcggttgatg aagcacgtca attactatac ggggcgaact atcataaagg
    37861 tgaagtaaat gtctaccata tattaggaga tggcgaactg aaagcagcag attcaatatt
    37921 ccatcaagaa gcgacaggtc ctcataaaaa ccaagatcat gcgcatgtcc actatacgga
    37981 tctgactcct gaccaacgat tagtcgtatg tgatcttggt acagatcgtg tgtatactta
    38041 tgatgtgtcc gaaaacggca aattgaatct tgtaactacc ttcactgcag agccaggaac
    38101 aggccctcgc catcttgtct ttcatccaaa tggacaattc gcttatttat ttggtgaatt
    38161 agacagcaca gtccgcgtgc tttcttataa taaagaaaca ggtgcttttg aagaattaca
    38221 gaaaatatcg accttgccag aagaattcga cggtgaaaat ggaggggcag ctatccgcat
    38281 ttcaaatgat ggcaagttcc tttatgcttc taaccgagga cataactcga ttgctgtatt
    38341 cgcagttttt gaagatggtt cccacattca aaacaagcaa atcatctcaa cagaagggga
    38401 ttttccccgc gatttcgcac tagatccaag caataatttt gtcgtgtgtg ccaatcagaa
    38461 caccgataat ctgacattat atcgccgtca tcctgaaaca ggattactgg aattactgaa
    38521 aaaagatgtc tatgtaccag aatgtgtctg tgtttatttt gatcgttagt aagaaaagga
    38581 gaatgaaatg aatcagcaag acgtattaga cagactacgg gaagaactaa atattccttt
    38641 ctttgatggg aaaatcgaag ataaagagta tagcgaagag gaatatcaga aactcaaagc
    38701 cgatttacag aactactttg aacaatatgt acagaatgta gaaaactaat cagctttcca
    38761 tactatctga aaagaacatc aggacaagac ttttccgtct atcctgatgt tcttttctac
    38821 aaataaaaaa ccgccgagat ggttagactc gacggaaaag gagtatttac tcgattttga
    38881 gtaagtacaa ggttgttgtt ggttataggt atgtcattat cataaaggat tttgatcact
    38941 ctatcataaa ggtaatatta catttatatt agataactct agtttttatt gttatcaaag
    39001 atttctttta ttttttcaac aacaaattag tcattttttg gatggcagct gtcaattggt
    39061 ctgtcgcttg gtcttgtttt gcttcagatg tattgaatag atcttcatct acccctgatt
    39121 caagatcgat actttggatc atttcttcac agcctgctac atatgaaaga taagcttttt
    39181 caaaattttt atgaatgccc attgcttttg ctggtggacg caaagtactg actttcataa
    39241 gcattgcccg atattttttt gttccttctt caaataacag gatgatctct tcaagagtcg
    39301 atttgtccag ttcacttgtt tttccttcat caattgcgtt tctgatcact tcatatttcg
    39361 gatgcattcc ttctccgatt gcttctgttt ccttgacgat ttgatcgatg gactgcacat
    39421 aatagccaat attcggtaac atgttcgttt tcctactttc tccattgttg ttattattta
    39481 ttctacttgt ttttatcctc aaatgacaag tacttgaaaa aatttaatgg tttctataca
    39541 gctcgccatt cttattcgtg ctcaaagaaa ccatctgcta aactatattg tttccctgcg
    39601 acaatgtcat attggatcaa ttgatattcg tcgtaaatct cttgttgcga gcgattgaat
    39661 tgcgcttctt tataccactg tccgttttga tagtacagtt ctcgttcaaa cgccggaagt
    39721 tcctgttcta atgataaaag cagctgataa aagccatttg ttgtctgatt cgtctgttcc
    39781 attagattcg ctgcaaaata aaacggactg gtgattgcat cgtgagtcgt ttgcttttct
    39841 aattctcctt tactgtcgaa cataagaaat tgtgtttcat gcaatgtttg tttgtcattt
    39901 tttgcttgga tcgtatctga atagatccct ggaagatgat ccccccaaaa aacaactaaa
    39961 gtccttctag aaagattctt caattcttcc gtgaactgct tcagtgccgt gctgctatag
    40021 gaaatatctt gcagataatt ctctaagtca agtgtcccac ttccatctga agtttttgca
    40081 ggatagtcta gattatcata ctttccatcg tagggcatat gtgtctgcat cgtgactaaa
    40141 tgaatgaact gaggtgtttt ttcttccttg agaagtgtca ttacttcttt gtaagctgac
    40201 tcatcagaaa tatatggatt attctctatc gtatccgtat aggtcatcgt acgctcgctg
    40261 atgaactggt caaatcctaa tgtctgataa acatcctctc ttttatacat cgaagtattg
    40321 tatggatgga tcgctgtcgt ctggtatgat tgtgcgttta acgctgaaac gatagaagga
    40381 agctgatcca tttttggtac aagcatcgtg taaggggtgg tgagttgcgc attaaataac
    40441 gccatagaaa aaccagtcaa tgcttcaaat tcgatatttg ctgttccacc gccatagttt
    40501 tgagaaagca tattgccact atatgtttga tcagctactt cataataatc tgccaatggc
    40561 tcaccagata cttctacgcc gtttaatcga ctatgatctg agaaactttc actcatcacg
    40621 aacacgatgt tcggcgactc ttcctctaca gttttatttt tttcatcggc tagcttctgg
    40681 tatttttcag tgatttcttt gatcttttct ttcgaatatc cttcaggttc atccattggt
    40741 tctaccttca gattgtacaa aaacccaccg ataaaaccag tgttatagta gttcattttc
    40801 tggctgtaag gtatccatag tgccgtttta ttatatgctt ttcgcaacaa attatcggga
    40861 ttgttgaaat tactgatata ccctagcagt ccaattgcca caagcagtgt cgttaatcgg
    40921 atcaattgga atcgccttcc ttttttcagg cttcgaaaca tgtaccagca aaacaatcct
    40981 atcacggttc ctgcggcagc taagatcact gtaaacagca tagtgccggt catatctttc
    41041 agcaatccga tttcagtgat catcttcaaa tcgtctggat aaatcggttc ttgccgataa
    41101 ctcattttca tataatttgc aaaacctaat acaccgatgg ctacgacata cgtaagggaa
    41161 cctaaaggga cagatcctgc tagtgcaatc agaaaaataa gaataatcag caagacgaga
    41221 caggctaaga agaatttttc cgtatgccaa gaaaaagcaa atttcaacgc aaggtctacg
    41281 gatagatcat tttgacacca ctgcaaaaat aaattactaa aaacaacaag aaaaaagata
    41341 ctgatgattg ctgctgttcc ttttaaaatc tttttctgtt tggtcgaaag tctgcttata
    41401 tatcttttca aatgcttgct ccttcatttc tatcaataaa agaatgttgc ttctcttatt
    41461 ttagttgtac gaaattcgaa agtaaagaac ggaaagaaga attaatgaaa tccaaatatt
    41521 ctctttcttt atggcctttc tggtttcttt aataagcgga cctttcgtac tccagaaatt
    41581 ttttctagct gttgtttcac ttgattgagc tgttcaaaat cagtactgtc tatgtcaatc
    41641 aatatgtaga catgctcatt cttatggtta cgcgatatat ctgcgatcgc tagctcgttc
    41701 tcattcagca gagaaaatat ctctgctaat atattggttt tcttttgata aaatatagtg
    41761 aatcgaaacg gcgcttggaa aaacattcgt gcggatggat aattgaccgc ttctctaacc
    41821 gtaccgaaca aaagataatc ttttaaggct tcaactgcca aacgtccgct gtctgccaaa
    41881 gcttcctgag tcgatccccc tagatgaggc aacataagta tacggtcatt ttctaagaac
    41941 tcttcttctg gaaaatcagt caagtattta gccagatgtt gtttttccaa tgcttgcata
    42001 acagcatatt tatcaacaat ttcacttctt cctacattga taagtacagc atcttttttc
    42061 attttttcta tattcgcctg atcgatcaat ccttttgtat cttctgtcaa cggcagcatg
    42121 actacgatgt aatccgaggt agaaagaact gtttctaaat ctgcttgccg aacataatcc
    42181 aactgggcat cgcggataga ataacccaag acatccatcc ctaagctata gcaacttagc
    42241 gctacttttg tgcctatcgc tccaagacca agaagaccga tggttttgcc ttgcaattct
    42301 cgtcccacat aagcacttct tttgttttct gcctgttcta aaatattagg acctgttagt
    42361 gtttgtacca tacgacttgc ttcgattatc ggacgggaag ataagagcaa acagcacaaa
    42421 acaagctctt ttactgcatt agcatttaca cctggtgtat tcatgacgat cgtcccgttt
    42481 tcagaagctt tttcaacgtt gatcgtgttc accccaacac cagctcttga aattgctagc
    42541 aaatctggtg taagccattc atctttcact ttcgtactac gaataactat tccttcggct
    42601 ggcaaatcat ctattatttt gaaatattcc ccttgtaact tttctatctc ttgttctgga
    42661 atagcatcaa tcaaatgaac gtgatacata aaaccccctc cttactaaca agtatctcga
    42721 aatttctgca attagacaac tgtttaccat ttgacttcct agaagcaaaa agagcagtta
    42781 gagcagagta ggctgagtga taaaaataag gctatgacaa gttactgcca taagccttat
    42841 tttcgagagc aaaatattga gggtgagata tcttgataaa tggtgttccg ttagctgaca
    42901 ttgataataa gactaaattt caaaaaagtt gaaaagcaaa tttcgtcaat ctcttcttat
    42961 ttcttaaaac taagtggctt tttcacaacc tctttttatc taataaccac tgtttccaac
    43021 tcaactaatt ttgcccacgt ttcaatttga tctgttgtta gatttagcga gacaacggta
    43081 tgatgaccgc cacctgcctg aatccatgaa tggacacctt catggaaatt cggtttagtt
    43141 ttccataaca cacgtgcaac aggtaaatta ggtgcttcag tcgttggttc aaaggcttct
    43201 acttcattga tcagcagttt ataatgcgta ccgaaatctg ccatcgatac gactacccct
    43261 tctcctgctt tcccatcaaa tacaagacgc gccggatctt ctctatctcc cattgataaa
    43321 ggagaaacaa cgattcttgg tttcgtcgca gcaagcgtcg gatctacttc catcatatgg
    43381 gattccaaga ttgcttcttg gccactagct agctcgtaag tataatcttc catgaatcct
    43441 gtatctttcc ctcgagccat gattttcagc aaccgatcaa tcgctgcagt tttccagtcg
    43501 ccttccccag caaaaccgta tccttctgcc atcaaacgtt gtacagctag tccaggtagt
    43561 tgtttcattc catacaggtc ttcaaaatta gtcgtaaaag cgttatagcc tccttcttct
    43621 aagaaacggc gaataccgat ttcctgctga gcttgtactt ttacatgttc ctcccatgtc
    43681 ttttcttcat aatcaccata gtcgaaggta tacaattcct tgtactcttc aaataattga
    43741 tcgacttctt cgtctgatac gcgatcgatt acttgtacaa gatctccgat accaaaataa
    43801 tcaacagtcc aaccaaattg aatctgtgct tccactttgt ctccttctgt gacacccacg
    43861 tttctcatgt tgtcaccaaa acgagccact tttattccaa aactttcatt ataggctact
    43921 gcaacatcca tccaatcagc aatctctttt tggatctcag gtcgtttcca gtagccaacg
    43981 acgattttat tttgtttatt taatcgagca ttgatgaacc catattcgcg gtcaccatga
    44041 gcggattgat ttaaattcat gaaatccata tcgatcgttt tccaaggaat gctttcgtta
    44101 tattgcgtag ctaaatgaag caatggtttt tgtagaagct ttgtcccgcg gatccacatt
    44161 tttgctggtg agaaagtatg catccaagtg atcacacctg ctacttcttc tcgataattg
    44221 acctctttca tgatttttgt gatcgtatca gcagtaactg ctaattcttg aaggcggatt
    44281 ggataaggca attggccatt ttcattcagt tcatcgacca tttcttgtgc atgtttcttt
    44341 acttctcgca gtgcttcttc cccatataaa tgctgtgatc ctacaacaaa ccaaaattct
    44401 ttttctccaa tattcaacat aattagccac tccttttatt tgttttgtcc gtaatacgca
    44461 ttttttccgt gttttcgtaa ataatgttta tctaaaatcc gttgaggcag ctcttctgaa
    44521 aatgaattga gctgtctggt aaataaattc atcttgcata cttcatctag tacgactgcg
    44581 ttcattaccg cactttgcgc atctttcccc caagtaaatg gaccgtgtcc gtgtaaaagt
    44641 acccctggaa ttgctaaggg atcaattttg cgttctttga atgtttctat gataactttt
    44701 cctgtttcat attcgtaccc gctgtcgatt tcctgttgtg tcaaaaatcg agcacaaggt
    44761 accgatccat aaaaagtatc tgcatgcgtc gtccccatcg caggaacatc caatcctgct
    44821 tgtgcccaga tcgtcgccca tgtcgagtga gtatggacga ttcctccgat ttgaggaaat
    44881 gaacgatata aaacagcatg agtaggcgta tccgaagagg gattgagttt accttcaatg
    44941 atatgattgt ccaagtccac aacgaccatg tcgtctgctg tcatctcttc ataactgact
    45001 ccgctaggtt tgataacgaa atatcgtgtt tcaggatcaa aagcgctcac atttccccat
    45061 gtatatttga cgagtccgtg tttaggcaag tcaagatttg cttggtatac ttcttctttc
    45121 agttgttcca gcattttcta tcactccttt ttcttgcaat tgatgaatcg ctgttttttc
    45181 aatgtcgatc ccctcacggt aacgtctcat aaatatctga tagcctgcta tttcttctgc
    45241 tgaaggtgtg atggtgattc cttggtcttg tccaaacacg tcttctgcca agaagttttc
    45301 taaagacttc ccatgtcgat ccatcatata ggctgctaat aacgcgattc cccacgctcc
    45361 accttctccg gctgtgtcca tcactgtaac tggtgcctcc atagcagatg ctagaatccg
    45421 ctgtccaact tctggtgttt taaaaatccc cccatgcccc actaaacgat caatttggac
    45481 attctcagat ttcaatatat ccattccgat tctcattgca ccaaaagcac tggctaaatg
    45541 gatccgcatg aaattcgcta aatcaaaacg gctacctggt gtacgtacaa ataatggcct
    45601 tccttcattt actcctgtaa tgttttcacc ggaaaaataa ccataagaaa gtaatccccc
    45661 gcaatccgta tcgccttcta gcgctttgtt gaataatacc gtgaataatt tatctctgct
    45721 tatctccata cccaaagaat tggtaaattc ttcaaaaata ctgacccaag cattgatatc
    45781 cgaagagcag ttattcgtat gaaccatgcc tactagttct cctgaaggtg tagtgaccat
    45841 gtcgatttcc ggatggacat tttctagttc tttttctaat acgatcatag cgaaggcgga
    45901 agtaccagcc gaaatgttcc ctgtcccttt ggctacactg ttcgtagcga ccataccggt
    45961 tcctgcatcc ccttctggcg gacaaaccgg gattcctggt ctcaaatttc ccgtagggtc
    46021 gatcagataa gcccctgatt cgcttaactt gccagcttct tctcctgcta ctagaacttt
    46081 aggcaaaatg tgatccaagt caatcgctac gtgcgctttt tcgattaact gaccaaaaat
    46141 atttttcatt tcttgatggt aggttttcgt tgaaggatcg ataggaaaca tgcctgatgc
    46201 atctcctacg cctagtactt tctcaccagt aagctgccaa tggacataac ctgcaagcgt
    46261 tgtcaaaaaa ttaatttctt tcacatgttc ttctttattc aaaatagatt gatacaaatg
    46321 agcgatactc catctctgcg gaatattgta ttgaaatttt tccgttaata ttcgttctgc
    46381 ttcttcagtg attgtatttc gccaagtacg aaatggtact agtaactgtt ccttgctatc
    46441 aaatgctaga taaccgtgca tcattgcact gaacccaatc gacccgattg tcgtaagatt
    46501 ctcgccatag atttcgaata cttctgctgc catttttcgg taactgacgc gcagtccttt
    46561 ccaaatatct tctaatgaat aggtccagat accattttct agtttgtttt cccactcata
    46621 actaccagcg gcaataggag taaaagatga atcaatcaat acagctttga tcctagttga
    46681 tccaaactca atccctaaag atgtacgacc atctatgaca tctaacatac tttgcgcatt
    46741 tacttccttc ataatgattt aactccctcc ttctcgtcaa acctgatcaa accgtctttt
    46801 tagtagcgct tacaatgatt attgtatact tttgtacgta catgtcaagc agttatttac
    46861 aagaatacaa taaaaaacac ttgttttgta cgtacataag ttataaaatg aaattgctta
    46921 caaaatctga tcaaccgcct tctcgaaatt ctagaaggag agaatactta tgaaaaaaga
    46981 aaaaaaagtt tcctcatcgt ttatttattt ttttggcgca tttggcggca ttctatttgg
    47041 atatgatatt ggtgttatga ccggcgcctt acctttttta cagcatgatt ggggtttggc
    47101 aggtaaagct tctttgatag gatggatcac gtcttcggtc atgttaggtg cgatccttgg
    47161 cggctcttta tctggtcttc tttctgataa gcttggacga cgaaaaatga ttcttctttc
    47221 tgcacttatt tttatggctg gttctgtcct ttctgcatct gcccctcaca atggttctta
    47281 ttttctgatt gcagcaagga ttcttctagg tttagctgtt ggcgctgcat ctgccctagt
    47341 tcctgcttat atgtctgaaa tggctcctgc tagactaagg ggaagattgt cagggatcaa
    47401 tcaagtgatg attgcttctg gcatgctcct ttcctatgtt gcagattatt tgctaaaagg
    47461 gttgcctgaa acgatggctt ggcgcgtgat gttagggctc gctgctgtcc ccgcgctgat
    47521 tttatttttc ggtgttttag ctctaccaga gtcaccgcgt tttcttatgc aatctggccg
    47581 cttggaagag gcaaaaagag tattgaacta tattcggaca ccaaaagaag ctgaacagga
    47641 attcgaacaa atccagctga atgtcaaaca agaaaagaca accggaactt cttggcatac
    47701 tttgttttta gaaaaatatc gttccttagt cattgccggt attggtgtag cagtattcca
    47761 gcaatttcaa ggagctaatg ctatttttta ctatattccg ctgatcgtag aaaaagcgac
    47821 tgggcatgca gcgagcgatg cattgatgtg gccgatcatc caaggaatca ttttagttgc
    47881 tggttctttg ctctttttgg ttattgcaga caagttcaat cggcgaactt tattgaaaat
    47941 aggcggtagc gtgatgggac tttctttcat cctaccagct gttttaggaa ctgtactgga
    48001 tgctcataca aattcgttgt tgattctctt gtttttatgt atttatgtcg cattttattc
    48061 ttgcacatgg gctccactga catgggtaat cgtaggcgaa atattcccac tagctgtccg
    48121 cgggcgagca tctggcctag cctcttcttt taattggatt ggttctttcc tagttggctt
    48181 gctttttcct gtgatgactg cttctatgtc tcaagaaatc gttttcggca tttttgggat
    48241 catctgcttt ttaggggttt tatttattca agagatcgta ccagaaactc gtgggaaatc
    48301 tttagaggaa atcgaacaaa gtgcatctaa aaaaacttat cctaaacgta tttctatcca
    48361 tcattcttaa agaggttgtg acagaagtgg tcagcttcaa gaaataagaa ggaaccgctt
    48421 agtttccagt cacaaggaac aatttcaaaa aacagcttct catgtttgat gaatattgtg
    48481 acttgtgacc agttggtagg agaacaccgt ttagtggaga ggtggtgaaa gaaacgttca
    48541 gccttgagcg atgacaaaag ctctcacaaa cattgcgacg agtaaccgta ggagcaataa
    48601 gaggcggtaa aaacccgtct cttatttttc gttttctttc actgtttccc gttcaactag
    48661 tacaggttca tagagaatat ttggctgtgc ctgtcctttt tcaatcgttg tgacgatcca
    48721 ccgtgcggca tcttgtccca acttttcttt tggatgcgtc aatgttgtca attttttatt
    48781 ccctaaatgg cttaaagaag aatcgtctgt tcctacgatc gataggtcat ctggaatatg
    48841 atagccattt tctgtcaact tatctagcat taaactagct atttgatcgt tatagcaaat
    48901 gatgccatct aaagcttgtt cttctttcaa ccgttgtact gttttttcag cgattgtttc
    48961 tcgtgtttcc gtggtgaaag taatgatatc ttccgtcaaa aaacgcacat tatactgttc
    49021 acacgcgctg ataaaacctt tcatcctgta ttttccttga agatcgtcga tttttgtgat
    49081 gaacaacaga cgctgatgtc ctttttttat caaatattct gctgccataa aaccgacttt
    49141 cacatcatct aaacaaatat aagaggcatt taactcttca taagcagcat tgatcatcac
    49201 catcggaatg ccttgttctc tcaaagcaac ataaagtgct aagtttgggt tatactgatt
    49261 acttttcgta ggctcaacga tcaaaccatc taccccataa gcaatcattt tcttcaaaca
    49321 ctctttctcc aattcgtgat cgttattcgt actggctaaa agcaatgaat acccagattc
    49381 tcgtaattca ttttcgattc ctcggataat cgacggaaaa atataatcag agagatatgt
    49441 ggtgatgaca ccgatcgttt ttgttctgtt caatggtgtt ttttctttct tatagctgtc
    49501 atctacatat gtgcccgacc ctttttcttt acgcagatag ccctcattta ccaacacagc
    49561 gattgcttgc cgaatcgtat gtctgctgac gtcatatatt tcttgcagct tgaattctga
    49621 gggaatggtt gtatttactt cgtattcctc acgaaggatc ttttgtttta tctcttctgc
    49681 aatatcttga tattttgcca tttgccttgc ccctttttat ttgtacgtac atatatcata
    49741 acgagtctgc aaggtttatt caagcaaaaa tcaaggtttc ttgtgctcat aaggcatacg
    49801 gaacaggttc accaaattct ggaaatcctt gctcattcca cgtaaagcat tgcgcgttcg
    49861 cgtgccgatt aggattgtct aacggatccc ctttttggtt cttctctggt cgagcatgat
    49921 agatcaaaat atcaaagcac ccgtcttctg aaaccgtaaa cgagttgtga ccaggaccaa
    49981 attgcttatt tttttcagaa gaaacgaata ctggctccga taatttatgc catgattctt
    50041 ttaataaaag attcttgtgc tcatcagccc acagcaggcc catggcatag ttttcatctg
    50101 ttgcactacc agaatacgta atgaatattt tcccattgcg agtaaggact gctggacctt
    50161 catttactga aaaaccccgt ttttcccatt catactcagg tatagaaagg aggatctgct
    50221 tcccctttaa cgtccaagga ttatccattt cggatagata aagattagaa ttcccagtaa
    50281 tttcaggatc ttgttgggcc cacacataat atagctttcc ttgagaagag aaagttgttc
    50341 cgtctaaact gaaagtttcc cttttcgttt ttatttgtcc cttttctatc caatctgtat
    50401 tcattggatc tatatgctga ttttcaagta cgaacatgcg gtgatgatga gattcatcac
    50461 gtatagtttt atgattgctt gcagcaaagt aaatatacca ttttccattg atataatgga
    50521 cttcaggtgc ccagatcaat tcactcatcg gtccttcttc atgagcctgc catacaatca
    50581 ggcttgcagc atgttctaat tcattcagtg actttgccct tcttaattca atcacttggt
    50641 agcctggaac cgatcctgta aaataataat aaccatctgt atgtttatat acccaaggat
    50701 ctgcccgttg ccgaacgata ggattattat aaagtgtctt catcataaca aatttcctca
    50761 cattttcttt attctaatta cattccaaga gtatttttct atccctacat ttaattcgtc
    50821 atgtttttct attgaaaaat agtttatttc aggttttatt ttttctggtt cttcacgtgt
    50881 atttttgtcc aacagatcgc tactggttaa tgtgtagtgc tctccttgat ctagcaaatt
    50941 gaaatcatgt tcaatcgtta tcttttgttg ttcatttgtg cgattgatca agaaaatagt
    51001 cagctgattg ccgttatcta cgatcacact gtccacataa ggaacatttt ttatcttttc
    51061 acaatcataa acagggcaaa tgatttgttg tttcaatact tgacctttag catatttcga
    51121 taagtgttgc atcacataat aagttggatt cctccatgct tgtcctttag catctgtcag
    51181 tattaaaggt attgtgttta ctaacaatga ttgacaacta atctttacgc gatctgcgtg
    51241 tcgtaaaata gccagcccta atgaaccaaa taataatgta tcttccatag taaagtaaca
    51301 ccaatcgatg ggactaccga cttcaaaatc cctatgtctt ttttccggtt gggcatgtac
    51361 attccattca tcaaacgcta gatacattgt tttctcactt ctcaaatatc cttttacata
    51421 atcacaagta gcaacgattt cttcaatttg acgatcgaat cgataagctt ttgcaaggta
    51481 tgtcgctgta tcatcacgct catttttagg aggtttagtc aagtcttctt catcatactt
    51541 atcaatatag ttatgcaaag ctaaataatc tacattctca taagcttctt ctaatgcgat
    51601 acgatcccat tcagggtacg atggtaaacg tggcgtagag cttccaacta agattgtttc
    51661 aattgaatca tccactaact tcatgacttt tgaggcttca tttgctaacc taccatattc
    51721 ataagcactc ttagccccca cttgccatgg accgtctagc tcattcccta aacaccataa
    51781 tttaacgcca aaaggctttt tttgcccatt ttttattctt aaatcactcc agaaggttcc
    51841 ttttggaaaa ttacaatatt ctactaaatt ttttgcttca tcaattcctt ttgttcctaa
    51901 attcaccgtg aatatcggtt tcgcttgtac atgactgatc catttaaaaa attcgtggag
    51961 tccaaaagaa tttggttcta tacctcgcca tgctaaatca attttttttg gtcgttgttc
    52021 tttcctacct acagtatcca cccaatcata accagatgta aagtttcctc caggatagcg
    52081 aatgacacct aattctaatt cctttacata agagaggaca tcttgtctaa acccgtcacc
    52141 atcagctgtt tcatggcccg gttcataaat ccctgtatag acgacacttc ccatatgctc
    52201 aacaaatgag ccaaacagcc gagaactgat ctctcctacc gtaaaatcct ctctgatttt
    52261 tactttcact tcttcgtcca acagctttcc ctacctttca tttcctatag tcaagataat
    52321 agatacaagt ttccatacgc caccagcaaa gataaaaata aaaggaggga aaagaaatat
    52381 gagcagtaca aatcctaata acaatacgca acaaagaata tttctcaaaa aatcaaaaaa
    52441 tactttccat gcactttgtt caaaaatttg taaaaatgtg atatctttgt tttcagaaat
    52501 cacatatatc caggataaaa gaaacatgga ataaataagt aaaactacat atccactaat
    52561 tgaaagtacg attgtctgtt cttctaataa ttgattaaca agaaaataaa taatgatcgt
    52621 taacagacta aggaacaagt tcttaccaaa atgttgcttt attccttgaa agaaaaaata
    52681 aatgatattt cctgatccct cacctttcca ttgcgccatt acataaaaaa cagcacatgt
    52741 agcaggcacg atagtaataa caggcaaaca cacgattacc cagagaatac ctaataagat
    52801 gtaatcagaa accacaatca actgtccata aagtttagtc tcaactattt tcatcttcct
    52861 cactctttca ttccagacat agctgttcct tgaacaaaat atttctgtgc aaaaatataa
    52921 accactagta atggcagcat tgaaataacc gttccagcca ttacaggtcc ataaaaagaa
    52981 atgttctgcc ctgtaaataa agctaaccct gctggtaaag ttctcatttt tgaactactt
    53041 gtcaatatca atggataaag caagtcattc caactattca tcagcgtgaa aatccctaat
    53101 gataaaagag taggtttgct caatggcaac atgattttca aaaaaatatt aaactcactt
    53161 aatccatcaa tgcgtgctgc ctcttccaaa gaatctggta aagtaataaa aaaggaacgc
    53221 atcatgaaga taccaaatgc agttgtcata cgaggaatga tcaaacctgc ataagtatca
    53281 agcatgccaa gcaaattgac ttcaataaat agaggaatca tgaagacttg gaatgggatc
    53341 atcattgtca gcaaaacgaa atagaacaaa actgattttc ctttgaaacg catacgagca
    53401 aatgcatacc ccgcaagaga atcaaagaat acagaagtga tcactgtccc tccagcaaaa
    53461 atcactgtat ttttgatgta atctaataaa gggatcgttg tccaaacctt aatatagtta
    53521 tctagggtat agctatctgc aaatattcta ggcggaaatg agataatgtc tttttcgtgt
    53581 ttaaaagaag aaataatcag ccacaaaaaa ggaaacgcaa taaccaacaa tataactagc
    53641 atactaatcg ttcgaatgat ttttttacca ctaactgctt ttcgactatt cacagtaact
    53701 gtaatcgagc tttctggtgc catacaggtt tccctcctat ttcatttgtt tttctttccg
    53761 tgacatgtac acattagtgg agatagctat agttgcaaca atgataaaca aaaccgaaga
    53821 cagtgctgat gcatagccca attcataagg tgctgtaaat cctcgactgt aaatatattg
    53881 aacagctgtt tctgtcttaa attgtgggcc accagcggtt gcaacatata cttggtcaaa
    53941 cacttgcatc gaaccaatca gattcgttaa aatacaaaaa cctaaggagg gaacgatctg
    54001 aggtagcgta atgttaaaaa actgctgtcg atttgttgca ccatccattt gagcagattc
    54061 atacaaagag ggagagattc cctgaatagc agtcaagaga atcgtcatag taacaccaaa
    54121 attcttccaa acagtcatga tggctaccgt cggcatagca agggttggat cgcgaaaaaa
    54181 ttgagggatc ggcaagccga cttgtgaaaa caagtaagga atcgccccta cattcggatc
    54241 aagcaacata gaaaaaataa tcgcaattga cgtcaatgaa caaataactg gaatgtaaaa
    54301 tgtcgaacga caaaaacgat tgaatctggt atttttagat aatgctactg ctgctaataa
    54361 accaacgata atttgtgttg gcgtttcgag tacggtgaaa tataaagaat gaatcaacga
    54421 attaactgct cgttcatcac taaatatacg tgtgaaattt tcaaaactta caaaatttct
    54481 tgatgtaaag aaaatattca aatctgtaaa actaatagca aatgttccta aaagcggtat
    54541 gacagtaaat aataataaaa ctaaaaaaga tggagttaaa aacaaatacg cagcacgatc
    54601 aggacgattc caatatttgg tgaatccgtt catatagtcc acctcatctt tcattttgat
    54661 aaaatataca atcttggttt aaacgaatct gaataagata tccgaacaat cgctgtgatt
    54721 tttgcaataa cacctatctg caaatactag gtcgatcatt cagatatctg tatcttgcca
    54781 acagtctatc ctgtataaag ttctgtacta tttttgcagt aattgattga tggcatcatc
    54841 tgcatcgttc atgagtgtct caggatcttc tcctgctaat aatttttcaa tcagtggatt
    54901 gatgatatcg ttattgatcg cagagatttg ggtaaaacct ggtaaatatg gctctgcata
    54961 gtccatttgt ttcgataatt cagatacaat aggatcattt tttacttctt catcttttgc
    55021 tactgaatcc aagtaggcag gaaaaccatt ttcgatactc cattttttac caatttcagt
    55081 tgtattccaa tatttcaaaa actcatagac agcttctttt ttattattat ctgtacttgc
    55141 cggaatggca tatcctacgc catctaaaat agctgttttt ttgtctgaat cactcccttg
    55201 tggtaaggta gtcacattat aatcaatctc gtttgctttc aatccgttat ttaaccatgg
    55261 cccattgatt tcaatggcta gttgaccagc attcattaaa ttgtccattt cggcgccaga
    55321 aatcgattct ggaccagtct tgtctacatg gatcattttt tggatcgctt ttaacgtttt
    55381 aaggttctca ggagacgcaa attcagactt agtataatca tcgttcacta atttaccgcc
    55441 atttgctaac atccagttat ataacggtgc agttccatcc ttattgaaga caaaacctga
    55501 tacattttta ctactatcag ttaattttgg tgccatttct gtcaattctt cccaagtagt
    55561 aggtggttgt tcagtatcta atccagcttg tttaaacaaa tcattattcc aatacatata
    55621 catgccttgt acctgcatgg ggacaaaata ctgtttacct tctacttcac ctaattcaat
    55681 agccgtttct ttaaaatcag atttatctac tccatcatat tcccagaaat cgtctaatgg
    55741 ttgtaccgct ccgttttcta catactgtgc aaaatctgga taatttaatg caataaagtc
    55801 aggtgctttt ttagaagaaa tcgctgtagg aagtttttca ttcaaattgg cccaagtcat
    55861 gatatccatt tcaattttga tgtttttatc atttgtttta ttaaattcat taacgatttc
    55921 ttgcaatatt tcaccatcag atgcagtaaa cccattccaa aaagtcaaag tcacttcttt
    55981 atcagaatca gattctgctg aacttccgcc accgcaagct cctaaaatac ctgttgcagc
    56041 taccaacaaa gagccaatta accatttttt acctttcatt ttttgttcct ccttcatctg
    56101 ataaacgaca tctgaccaca ttccaagata gcggtttcaa taaacaattg attttcccat
    56161 cttcagcttt tacatttgtt gaatgacttg gttttacttg ttcactttta ggaagattag
    56221 ttgcttttgt atcaaatcca ctcatttcag aaaaatcaat cactccctct aatgataatt
    56281 cagacaagtg aacagttaga ttcatttctt cttctccacg gttaactgca aatatgacaa
    56341 gttctttgtc atttattacg gctatacttt ctaaatacgg cacttcttca aaatcttccg
    56401 tcgcatagtt tgctgaacga atttgtgggc ttaacacctc tccttgtcca tacacagcag
    56461 cttgcataaa tggataatag atcgtctgtc tccaagcttc tccgtttgtt tctgtcctga
    56521 ttggtgcaat aacatttacc aattgagcaa gacaagcaat ctttactcta tctgcatgtt
    56581 tcaataaagt gattaataga cagcctaata gtaatgcatc ttcaaaatta tagttatctt
    56641 ctagtaaagg aggagcaatc tgccaaggcg ctgcttgctc atcttgttcc gctgaatgat
    56701 accaaatatt ccattcatca aaggacaggt tgatctgctt cttactatgt ttcttcgcct
    56761 tgactgtatc gcacatcgct gtcacttcag aaatgaaccg atccatgtct aaggaacgtg
    56821 caagataatg agaaagatca tttttttggt tgccataata ttgatgcaga gacaaataat
    56881 ctatttgatc gtacgcttga tcaagaactg ttaactccca atcaccaaat gtaggcattt
    56941 gccgattcga acttccgcat aaaaccacct caattgaatc atctacgagt ttcatcgcct
    57001 tagccgtttc ctctgctaac cgtccatatt cttccgctgt tttatgtcca atctgccaag
    57061 gaccgtccat ttcattccct aaacaccatg tcttaattcc aaaagggctc tcttgaccat
    57121 tcgaacgcct taaatcactc caataagttc ctttaggaaa attacaatat tcgactaaat
    57181 tacgagctgc atctactcca cgagtaccca gattgacagc catattgatt tcggccccca
    57241 catgctgaca ccagttataa aattcatgaa tcccaacttc attcgtttca attgtccgcc
    57301 aagctaaatt caaccttcta ggtctatttg ctatgggccc aatactgtct tcccaacgaa
    57361 atcctgacac aaaatttcca ccaggatatc taatcatggg aatgttcaat tcttttacca
    57421 agtgaatgac gtcttggcga aatccacttt tattagctga tggatgatct ggctgataaa
    57481 tcccttcata tactgctcgt cccatatgtt caataaatga actgtacaac cgcggatcaa
    57541 ctttgcctac acgcttgaca ctactaatca acattgtggc ttccataact gacctccttt
    57601 aatttataca tgaataatac acctcgttga aagcgttgtc aataatataa atgttaaact
    57661 tacttatttt ttatcgattt taattaacca aaatattaat aatgattaaa acaaataatt
    57721 taatataact tttattaaca aatatgttaa tatataagaa aaacctttta agaggtgata
    57781 tatatgcaac tacaaataaa cgaagaatcc ttaccggtat atgaagcatt agctagtaaa
    57841 acaaggatca agatcatcca gctactttcc aaaaaaaaga tgaatgtcaa agatttggca
    57901 aaagaacttg gagtgagcag tgctatcacg acgatgcacg tgaaaaagct agaggaagca
    57961 aacatcatca aaacagaaaa agtaggacaa caaaaaattt cgagccttcg tgtcgataaa
    58021 atcgatatca gttttccaga aaaaatattc aatgcttttg atacgaaaga aacatcgata
    58081 cctattggtc actatacaaa ttatgcaatt gaaccaacat gcggtttagc tacgatccat
    58141 gattttatcg ggaaagtgga tgaaccacgc tattttatgg atcctcgaag gatggatgcc
    58201 cgcattctct ggtttaccag cggatttgtc gagtatcagg cacctaattt tttaaataca
    58261 gaggatacat tggagatgtt ggaagtttct gtggaaattt cttctgaatt tcctttttca
    58321 aatgacaatt ggccttcaga tatcactttc agcctaaatg gtgtggagct aggaacatgg
    58381 acgagccctg gagattttgc tgatatccgc ggtaaatata cgcctgattg gtatccagat
    58441 aatcttaatc aatacggctt gctgaagacg atccgaatta ccaaacactt gacgaatatg
    58501 aatggagaac ccttgtcaaa catcacgatc aacgacatcc ctaaagagca agacacttgg
    58561 catttgcgaa tcgaagtgaa agacgatgcc aaacatgtgg ggggctgtac tttatttggt
    58621 aaaggattcg gtaattacga tcaggatatc aaattaaaag tttactacag ctgatatttt
    58681 tcttttctat tttataaaac aaaaacagcc aatccctatg aagggacggc tgtttttgct
    58741 ttattgttat ttccagaaat catccaaaac ggaaattggc aaatgtcgtt tatgttgtgt
    58801 cttgttccac catgcttcga ttttttcttg tgcttgagcg ggaacttctt ttccttctag
    58861 ataatcatca atttcattat atgtgacacc tagtgcgact tcatctgcaa tcattggttt
    58921 cccatcttca agatctgcag tcggaatttt tgtgtataat ttttccggtg cattcaatgc
    58981 ttgaagcaat tgtttccctt gtcgtttgtc caaacggaat aatggcaaaa tgtcggctcc
    59041 accatcacca aatttcgtga agaatccagt aatgttttct gcagcatgat ctgtccctaa
    59101 tacggctcct gctctttctc cagctacggc atattgcgta atcatacgtt gacgtgcttt
    59161 gatatttcct ttgttaaaat cgctgattcc cacacctgct tcgcttaata cttgtacttg
    59221 tgcatcaaca gctggtttga tatttactcg cagactaaca tcgggctgga taaagtcaag
    59281 agcagctttt gcatcttctt catctgcttg ttcaccataa ggaagacgaa cagcgataaa
    59341 ttgatagttt tcatcttttg tttcttcacg catttcttcc atcgttagtt gcgccaaacg
    59401 gcctgctaaa gtagaatctt ggccgccgct gatacctagt acaaaagttt taagaaacgg
    59461 atattttttc aaatatgctt tcataaagtc aatactttta cgtatttctt cttttggatc
    59521 aattgttggt ttgacaccta attcattgat aatttctgtc tgcaggctca tttattctcc
    59581 tcctatgctt ctgtatgaag ttctgctacg ctttttttca ttcgttccat gatagccatt
    59641 ttatggttcc agcattcagt agacagatcg actgggtatt tttgcggatt caagtctcgt
    59701 ttatactctt cccatagtga atctagattt tcttttgtat attctttgat ttcgtttagt
    59761 gcaggtaatt cgtaaactct ttttccttcg ataaagatat cttgtaaaac tgggcgagct
    59821 gtaaagtctc gaacagtttt attgataaac gtatgcactg gatggaacat gaagatagct
    59881 tcttcttctc gagggtcttc atcccataat gtcacgtagt ctccttcaga tttcccatca
    59941 aaatttcgag tgatgcgcca cacttgtttt ttccctggag tagttacttt ttctgcattg
    60001 cttgataact tgatcgtatc catcattttt ccttcatcat cttcgatcga tactagcttg
    60061 aatactgcac caagggctgg ctgatcatag gctgtgatca attttgtccc aacaccccat
    60121 acatcaattt tagctttttg catctttaaa ttcaaaatcg ttgcttcatc caagtcattc
    60181 gaagcataga tttttgcttc cgtaaatccg gctgcatcta attgttcccg aacacgctta
    60241 gatatatagg ccatatctcc actgtcgatg cgtacgcctt ggaagttgat tttgtcacct
    60301 aattctttcg ccacgcgaat cgcacttggc acacctgatt tcaacgtatc ataagtgtcg
    60361 actaagaaca cgcagtcttt atgggtttta gcgtaggcca taaatgcttc ataatcattt
    60421 ccataagatt ggactaacga atgagcatgg gtcccgctag ctggtatccc aaatatcttc
    60481 ccagcacgta cattgcttgt tgcatctgct ccgccgatat atgccgctct agttccccag
    60541 actgctgcat ccagttcttg tgcccgtcta gttccaaatt ccaatagtgg atcttctccg
    60601 atgacagatt tgatacgagc tgcttttgtc gcgatcaacg tttggaaatt gaccatattc
    60661 aacagtgctg tttctactaa ttggcagtgc gctaaaggcc cttccacttg gattaatggt
    60721 tcatttgcaa aaactagttc tccttcacga gcagaacgaa ctgttgcttt aaattcaaaa
    60781 ttttgtaaat actccaaaaa accttcagga tagacttcta atccacgcaa gtaagcaata
    60841 tcggaatcat tgaacgtcag attctctaaa taattgacta accgttccaa tcctgcaaaa
    60901 accgcgtatc cgtggttaaa aggcatttct cgaaagtaac attcaaaaac agcatgtaaa
    60961 tcagctcgac ctaattccca gtatgtctgc atcatattga tttgatatag atccgtatgc
    61021 agcgtcaaac tgtcgtctgg gtaagtatta ttcatcttgt attactcctc ttggcaagtt
    61081 ttcatttcct acccattata acaaaaaaaa ttaataggaa aagaaatctt ccattttttc
    61141 tgaaaaagca catgttttac ggatatttct tgctaaaaat gaactccatc tgctaaaact
    61201 atcctttctt attggtattt aacctaaacg agaaagaata cttattttat tcaatgtatc
    61261 cgagacacga agaaattagc gttttctatt tttcttactt gataaaataa gtagattttc
    61321 ctactacaat cgtagccgtt aagataccga tgattgaaca aatataacca aatgtctcta
    61381 caacataatc aatggcaaaa gtttttgaag caataatacg tctttccttt tttgcttctt
    61441 atccccaaag aaacggatat aacttattat gtattgaatt tttttggaaa ggcgaattat
    61501 gacaagatat accttgcgtt gaaaaaactc gcggaaaaaa tatcacacgt ttttaaaaag
    61561 atacaaagta agaaagcagc tgtcaataga atggaaacaa aaatggcgag acatcaatca
    61621 gtctcgcctt gttttttatt gtatacatta gatatggagc aggttaccat aaagatgatt
    61681 attagtcgtt attaaataga atccttcgta tttttttgtt ctatcactac aatatcttta
    61741 tttggacgtt tgaattgtat tccggcacgt tctagctcct gtttcactcc tacatggact
    61801 ttgacatctt ctcgtgtcat ttttaaaacg tgatgaatcg catagtcgat ttcttgcctt
    61861 gtgatttttt tcttcgtatt gatcatcacg acttgctttt tatcatatgt gtctaccaat
    61921 agcagttgtg ttcgatctaa cgaaaataat ttgtaataat taatagccgt attcccaaaa
    61981 aagaattcca ctactttagg ataaaatgtc ttaatattta aattactatt cacaggtgtt
    62041 tcgattagct tcatctcatt cctcttttct atatagtcga tttagatcca atcttaagtt
    62101 atgttaccat ctttcctagc attaaggaat cggttacaat tgaaccttta aagacaaaag
    62161 gatttacctt ttatcactta atagtataac ataatttcgt gagaaaaaaa tacaattttt
    62221 tcttgaccat ctcgttacgt aaaggattat gcttacattt gtcaggagga aaaataatgg
    62281 agtacacaat aaagaaaatg tcagagatat caggagtcag tccgagaacc ttgcgctttt
    62341 atgatgagat tggactgcta aaaccagcta ggatcaattc ttcaggttat cggatttatg
    62401 gcaaaaaaga agtcgatcgt ctgcaacaca tcttatttta tcgaacaatg gcattcaagt
    62461 tggatgatat ccaagaggtc ttagacaatc catcttttga ccaccagaaa gctttaatca
    62521 agcatcagca aatgttgctg gaaaaacggg cacaaatcga taccctcttg acaactgtcc
    62581 aacagacact tgatatgtac gaaggaggaa gaaaaatgag tgacaaagaa aaatttgaag
    62641 ggtttaaaca acaaaagttg aaagaaaatg aagaatccta tggacaagag attcgacaaa
    62701 aatacggaaa aaaaaaggtt acagcatcca atgaaaaatg gatgaatatg gataaagaaa
    62761 cgtatgaaga aatgcaaact gttgaaaagc agatgttatt tgatctcact ctttacttaa
    62821 aagaaccaga agatgaaagc ttagcagacc gtatcttcca agctcataag aagtggttct
    62881 cttacagctg gcctgaatat cagccaaaag cgcataaagg actaggaatg atgtacgaag
    62941 ccgatgaacg gttcactgca tattatgacg agcgaagtgg tcatggtgca gcaaaagctt
    63001 taaatgaaat tatccaaaaa aaggcgtaat cttttacatg atccaacaaa aaagtacacg
    63061 taaaatgacc taataacaaa acagcagaca cccaagttag atttttatgc taacttttgg
    63121 tgtctgctcc attctttcac aagttttttc atcctacttt aaatatattc atcgttgaat
    63181 aaacgtataa ggtattttca ctttatcctt tttttctgta agaaacaatt gaaatgccat
    63241 ttctccgcaa gcttccagat ggtggtcgat ggttgggaaa ccaagaagct cgctgatcag
    63301 caaattatcg cgtccaatca ccactggtgt cttatcttgc agatagcttt gcaagatgcc
    63361 tgctgcaacc atatccgaat ttgtaaagac accatcaact ccaagctctt taaaaaagaa
    63421 gcctgcttgt tttcctttct ctgcatctat acaatcccaa aaaacaagtg attcatcaaa
    63481 agatggaaaa agttgatgac atagctgcaa agtgattttt gagttataac ttagcctgcc
    63541 acttcgcccc aacgtgatgc ccaattgctt tataccttgc tgcttcaaat aagtaagacc
    63601 ttctgtaatt gattgttctc gatcaataaa gacacagctt gcctgttttc ccttgatttc
    63661 ttcacaaaaa actagaggtc catatttttg atatgccaat aaatcatcta gtgtattggc
    63721 acgagacgta atgatcaatc catcgaaagc tttagctgca aattcttcaa gataccttcg
    63781 ctctttttct ttatgatagc ccgtcggtaa aagtgtcact ttgtaatcat aatcaaacgc
    63841 tgcttcaatg atcccactga tcaactgatc aaaatacgga tgattggtat aaggcagaat
    63901 cactccaatg cttttcgttt ttccataact taactgtctg gcatttgcat tagggatata
    63961 atcgacttct ttcatgaccg caagtatttc tttccttttt tcctcatcta catatggatg
    64021 tccattgatt acacgtgaaa tagtagaaac tgaataacct gtgagctttg cgatatctcg
    64081 aatgtttgcc actgctgctt cgccacctat acaaaaagat tttcttgtaa atagtctacc
    64141 acagctggac cgcattcaaa aaactgatac atttttcctt catgcgaaaa actgatccgc
    64201 tgcccgcccc aatatatatc ttttgtcaaa ttttcgattt cattcattgg tatttctttt
    64261 actatattcc tattcaaccc ttgaaaactg tacaacgtta atacggatcc cttgaactcg
    64321 acaaacgcat aaaaataatt gttcgttgtg ccatctgtta tctctacata agtattcatt
    64381 gcaagccctc cttttgatac tttgagtata gtgtatgaaa cgcgtctcat agcaaggagt
    64441 ttgttttgcc aaagaaaagt tttattcctc tttcattatt accaaagtct cttattttaa
    64501 attgaataca ttataatgat actgtaaata tatctttatt attagtgaat g
//
LOCUS       DACDPS010000008        59728 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.008, whole genome shotgun sequence.
ACCESSION   DACDPS010000008 DACDPS010000000
VERSION     DACDPS010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.008"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..805)
                     /locus_tag="IWJ07_000561"
     CDS             complement(<1..805)
                     /locus_tag="IWJ07_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9332164.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSLKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVK"
     gene            complement(1029..2969)
                     /gene="thrS"
                     /locus_tag="IWJ07_000562"
     CDS             complement(1029..2969)
                     /gene="thrS"
                     /locus_tag="IWJ07_000562"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAP9332165.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3306..3485)
                     /locus_tag="IWJ07_000563"
     CDS             complement(3306..3485)
                     /locus_tag="IWJ07_000563"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAP9332166.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3532..5355)
                     /gene="cadA"
                     /locus_tag="IWJ07_000564"
     CDS             complement(3532..5355)
                     /gene="cadA"
                     /locus_tag="IWJ07_000564"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP9332167.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5633..7912
                     /locus_tag="IWJ07_000565"
     CDS             5633..7912
                     /locus_tag="IWJ07_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9332168.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8005..8652)
                     /locus_tag="IWJ07_000566"
     CDS             complement(8005..8652)
                     /locus_tag="IWJ07_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332169.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8831..9550)
                     /locus_tag="IWJ07_000567"
     CDS             complement(8831..9550)
                     /locus_tag="IWJ07_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9332170.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9681..10832)
                     /locus_tag="IWJ07_000568"
     CDS             complement(9681..10832)
                     /locus_tag="IWJ07_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAP9332171.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11049..11789)
                     /locus_tag="IWJ07_000569"
     CDS             complement(11049..11789)
                     /locus_tag="IWJ07_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9332172.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11793..12116)
                     /gene="rsfS"
                     /locus_tag="IWJ07_000570"
     CDS             complement(11793..12116)
                     /gene="rsfS"
                     /locus_tag="IWJ07_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAP9332173.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12173..12766)
                     /locus_tag="IWJ07_000571"
     CDS             complement(12173..12766)
                     /locus_tag="IWJ07_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9332174.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12750..13397)
                     /locus_tag="IWJ07_000572"
     CDS             complement(12750..13397)
                     /locus_tag="IWJ07_000572"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAP9332175.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13410..13721)
                     /gene="yhbY"
                     /locus_tag="IWJ07_000573"
     CDS             complement(13410..13721)
                     /gene="yhbY"
                     /locus_tag="IWJ07_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAP9332176.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13737..14846)
                     /gene="yqeH"
                     /locus_tag="IWJ07_000574"
     CDS             complement(13737..14846)
                     /gene="yqeH"
                     /locus_tag="IWJ07_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAP9332177.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14843..15370)
                     /locus_tag="IWJ07_000575"
     CDS             complement(14843..15370)
                     /locus_tag="IWJ07_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAP9332178.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15550..16335)
                     /locus_tag="IWJ07_000576"
     CDS             complement(15550..16335)
                     /locus_tag="IWJ07_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAP9332179.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16328..17197)
                     /locus_tag="IWJ07_000577"
     CDS             complement(16328..17197)
                     /locus_tag="IWJ07_000577"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAP9332180.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17198..18577)
                     /locus_tag="IWJ07_000578"
     CDS             complement(17198..18577)
                     /locus_tag="IWJ07_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAP9332181.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18585..19004)
                     /gene="fabZ"
                     /locus_tag="IWJ07_000579"
     CDS             complement(18585..19004)
                     /gene="fabZ"
                     /locus_tag="IWJ07_000579"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP9332182.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19029..19505)
                     /gene="accB"
                     /locus_tag="IWJ07_000580"
     CDS             complement(19029..19505)
                     /gene="accB"
                     /locus_tag="IWJ07_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAP9332183.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19508..20743)
                     /gene="fabF"
                     /locus_tag="IWJ07_000581"
     CDS             complement(19508..20743)
                     /gene="fabF"
                     /locus_tag="IWJ07_000581"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAP9332184.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20762..21499)
                     /gene="fabG"
                     /locus_tag="IWJ07_000582"
     CDS             complement(20762..21499)
                     /gene="fabG"
                     /locus_tag="IWJ07_000582"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAP9332185.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21511..22428)
                     /gene="fabD"
                     /locus_tag="IWJ07_000583"
     CDS             complement(21511..22428)
                     /gene="fabD"
                     /locus_tag="IWJ07_000583"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP9332186.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22535..22759)
                     /locus_tag="IWJ07_000584"
     CDS             complement(22535..22759)
                     /locus_tag="IWJ07_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP9332187.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22812..23774)
                     /locus_tag="IWJ07_000585"
     CDS             complement(22812..23774)
                     /locus_tag="IWJ07_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAP9332188.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23784..24224)
                     /locus_tag="IWJ07_000586"
     CDS             complement(23784..24224)
                     /locus_tag="IWJ07_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9332189.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24530..25495
                     /locus_tag="IWJ07_000587"
     CDS             24530..25495
                     /locus_tag="IWJ07_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP9332190.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25552..25782)
                     /locus_tag="IWJ07_000588"
     CDS             complement(25552..25782)
                     /locus_tag="IWJ07_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAP9332191.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(25868..26755)
                     /locus_tag="IWJ07_000589"
     CDS             complement(25868..26755)
                     /locus_tag="IWJ07_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP9332192.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTNTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(26844..28067)
                     /locus_tag="IWJ07_000590"
     CDS             complement(26844..28067)
                     /locus_tag="IWJ07_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP9332193.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28084..29163)
                     /locus_tag="IWJ07_000591"
     CDS             complement(28084..29163)
                     /locus_tag="IWJ07_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP9332194.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            complement(29294..31021)
                     /gene="ptsP"
                     /locus_tag="IWJ07_000592"
     CDS             complement(29294..31021)
                     /gene="ptsP"
                     /locus_tag="IWJ07_000592"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAP9332195.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(31021..31287)
                     /locus_tag="IWJ07_000593"
     CDS             complement(31021..31287)
                     /locus_tag="IWJ07_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAP9332196.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            31766..34000
                     /locus_tag="IWJ07_000594"
     CDS             31766..34000
                     /locus_tag="IWJ07_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP9332197.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            34148..34468
                     /locus_tag="IWJ07_000595"
     CDS             34148..34468
                     /locus_tag="IWJ07_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP9332198.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(34673..34896)
                     /locus_tag="IWJ07_000596"
                     /pseudo
     CDS             complement(34673..34896)
                     /locus_tag="IWJ07_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
     gene            complement(35094..36674)
                     /locus_tag="IWJ07_000597"
     CDS             complement(35094..36674)
                     /locus_tag="IWJ07_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAP9332199.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(37075..38451)
                     /locus_tag="IWJ07_000598"
     CDS             complement(37075..38451)
                     /locus_tag="IWJ07_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP9332200.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(38579..39697)
                     /locus_tag="IWJ07_000599"
     CDS             complement(38579..39697)
                     /locus_tag="IWJ07_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9332201.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            39839..40273
                     /locus_tag="IWJ07_000600"
     CDS             39839..40273
                     /locus_tag="IWJ07_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9332202.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(40351..40884)
                     /locus_tag="IWJ07_000601"
     CDS             complement(40351..40884)
                     /locus_tag="IWJ07_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAP9332203.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(40978..42156)
                     /gene="mutY"
                     /locus_tag="IWJ07_000602"
     CDS             complement(40978..42156)
                     /gene="mutY"
                     /locus_tag="IWJ07_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAP9332204.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(42149..42946)
                     /gene="recX"
                     /locus_tag="IWJ07_000603"
     CDS             complement(42149..42946)
                     /gene="recX"
                     /locus_tag="IWJ07_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAP9332205.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            43053..44435
                     /gene="rlmD"
                     /locus_tag="IWJ07_000604"
     CDS             43053..44435
                     /gene="rlmD"
                     /locus_tag="IWJ07_000604"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP9332206.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(44609..45187)
                     /locus_tag="IWJ07_000605"
     CDS             complement(44609..45187)
                     /locus_tag="IWJ07_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAP9332207.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            45509..45979
                     /locus_tag="IWJ07_000606"
     CDS             45509..45979
                     /locus_tag="IWJ07_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAP9332208.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(46087..47313)
                     /locus_tag="IWJ07_000607"
     CDS             complement(46087..47313)
                     /locus_tag="IWJ07_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAP9332209.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(48067..48267)
                     /locus_tag="IWJ07_000608"
     CDS             complement(48067..48267)
                     /locus_tag="IWJ07_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9332210.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(48401..49009)
                     /locus_tag="IWJ07_000609"
     CDS             complement(48401..49009)
                     /locus_tag="IWJ07_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAP9332211.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(49094..49564)
                     /locus_tag="IWJ07_000610"
     CDS             complement(49094..49564)
                     /locus_tag="IWJ07_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAP9332212.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(49612..50526)
                     /locus_tag="IWJ07_000611"
     CDS             complement(49612..50526)
                     /locus_tag="IWJ07_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP9332213.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            50672..51475
                     /locus_tag="IWJ07_000612"
     CDS             50672..51475
                     /locus_tag="IWJ07_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAP9332214.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            51653..52600
                     /locus_tag="IWJ07_000613"
     CDS             51653..52600
                     /locus_tag="IWJ07_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP9332215.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(52668..52961)
                     /locus_tag="IWJ07_000614"
     CDS             complement(52668..52961)
                     /locus_tag="IWJ07_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAP9332216.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(52989..53654)
                     /locus_tag="IWJ07_000615"
     CDS             complement(52989..53654)
                     /locus_tag="IWJ07_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAP9332217.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(53794..54426)
                     /locus_tag="IWJ07_000616"
     CDS             complement(53794..54426)
                     /locus_tag="IWJ07_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9332218.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(54433..55500)
                     /locus_tag="IWJ07_000617"
     CDS             complement(54433..55500)
                     /locus_tag="IWJ07_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9332219.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(55497..56228)
                     /locus_tag="IWJ07_000618"
     CDS             complement(55497..56228)
                     /locus_tag="IWJ07_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAP9332220.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(56397..56876)
                     /gene="greA"
                     /locus_tag="IWJ07_000619"
     CDS             complement(56397..56876)
                     /gene="greA"
                     /locus_tag="IWJ07_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAP9332221.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(57012..58187)
                     /gene="mltG"
                     /locus_tag="IWJ07_000620"
     CDS             complement(57012..58187)
                     /gene="mltG"
                     /locus_tag="IWJ07_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAP9332222.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            58391..59560
                     /locus_tag="IWJ07_000621"
     CDS             58391..59560
                     /locus_tag="IWJ07_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP9332223.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
BASE COUNT        17316 a        12563 c        10090 g        19759 t
ORIGIN      
        1 ttttcacttt ttcgctaagc ttttcgtttt tagccatata gaaataaaaa atgatatcga
       61 tcgtagcaaa ttgggccaaa agtgaattag ttgccgcact tcgattcgtg atttcttttg
      121 gtctagatgt ttgtacgagt acatcggcta tcttgctaag cgggttattt ccaaattgcg
      181 tcaacgcaat gatttcgata ttcatgcttt tcgccagctc ggctaatgcg actacatctg
      241 cacttctacc tgagtttgaa acaagccaaa gcactttctt tttctcattg ctgacaagtt
      301 gcgggagcaa gacgtggata tctttttcaa aaatgattgg cttaccgaca cgagaccatt
      361 tttgcagaat atcttctact actaaagaag aggcgccaac cccatataaa aaaacagtct
      421 ctgccgattc caaacactct acgacttttt ggatcgtttc ttcttctaga atatctactg
      481 tttcttttat cgctacttgt gcattgttac caagtttgtt tttgagagag taaaaggact
      541 catttgcttc tatatcacta aaaccttcat cgattccagt ctgtagttct gttgaaatcg
      601 tgattttgaa gtccgtcaga ctttgaaaac cgattttttt ggaaaaacgt acaactgtcg
      661 gcgcactcaa cccggaagct tctgctaatt cattagctgt cattgtaggg accttctcaa
      721 tattaccgat aatgtagttg gccaactttt tttctgctga agaataatct tccatcatac
      781 tttcaatacg actaattacg ctcataagtt cctccaccct tttaatgata gtctatgtaa
      841 gtataccata tcagaccctt attcacggtc gtatactgga taaaaaacaa aaaaacaatc
      901 cgcccaacag aatatcttat cccatcaatc ccacagattc tattgagcat ctgtgtcttt
      961 gaggaatacc tgttatctat tgttcggatt gctttctatc tactactttt ttacaggaaa
     1021 tttacctatt agttttctct gctgtaattt ttgacttctg cttccatagc atcaataaag
     1081 atattcaaat cttctacagc tgtttcttta cttccataac gacgtacgtt cactgttgca
     1141 tcttccaatt ccttgtctcc aacaactaat tggtaaggga tcttttgtgt ttgagaggca
     1201 cgtatcttat agcccatttt ttcattacga tcatcaactt ctacacgcaa ccccttcatt
     1261 tgcaaacgtt ctttgatttc ataagcataa tcgccatgtg catcaacaga aacagggatg
     1321 attgttgctt gtataggtgc caaccatgtt ggaaaagcgc ctttgtatac ttctgttaaa
     1381 taagcgacaa aacgttccat tgtagaaaca attccgcggt ggataactac tggacgatgc
     1441 gtattttccc cgtcttctcc aacatatgtc aaatcaaagc gctctggcaa taagaagtct
     1501 aattgtatag ttgataatgt ttcttctgta ccaagagctg ttttgacttg aacatctagt
     1561 tttggtccgt aaaaggctgc ctcgccttct gcttcaaagt aatctagttc taattcatcc
     1621 atagcggctt tcagcattgc ttgggctttt tcccacattg cgtcatcatc aaaatattta
     1681 tctgtattgt ttggatcgcg atagctcaag cggaaacgat aatctgtaat attgaagtca
     1741 gcatatactg ccaccatcaa ttcaagcgta cgtttaaatt catctttgat ttgatcagga
     1801 cgaacaaatg tatgaccatc attcaacgtc atttctcgta cacgttgtaa acctgacaaa
     1861 gcacctgatt tttcatagcg atgcatcatt cctaattcag cgatacgaat tggtaattca
     1921 cgataagaat ggatcgtgtt tttgtagacc atcatgtgat gagggcagtt cattgggcga
     1981 agaacaagca tttcgccatc tcccatatcc attggcggga acatgtcttc ttgataatga
     2041 tcccagtgtc cagatgtttt gtacaactca acatctccca ttattggtgt atatacatgt
     2101 tggtaaccta gactaacttc tttgtctacg atataacgtt cgatcgtgcg tcggattgtt
     2161 gccccttttg gaagccagaa tggcaaacca gaacctactt ctggggaaac catgaacaag
     2221 tcaagttctt ttcctagttt acgatggtct ctttcttttg cttcttcacg gagacggata
     2281 tattctttca aagctttctt gtcaaaaaag gctgtcccgt atacacgttg catcatttga
     2341 ttgtttgagt ttccacgcca ataagcacct gccactgata ataatttaaa tacttgtata
     2401 cggccagtgg atggaacatg tggtccacga cacaaatcga tgaattcgcc ttgttgataa
     2461 gcagtaatca cttcttcttc tggcaattca ctaatcagtt ctactttgta cggatcactt
     2521 gcaaaaattt ctaatgcttc ctgttttgaa agaacacggc gttcgatcgg cagattttct
     2581 ttgacgattt tcatcatttc tgcttcgatt gccggcaaat cttctgcagt tacttggttt
     2641 tgtccattat ctgtatcgta gtaaaaccct gaatcgatag ctggtccaac gccaaagtgg
     2701 atatttggat agaggcgacg catggcttgg gccatcaaat gagctgctga atgacggact
     2761 aagccaagtg catcttcatg gtctggtgtg atgatctcaa tacttgcatc ttcctcgatt
     2821 ggacgagtca aatcaacgag ttttccgttc actttaccag ctaaagcttt ttttgctaag
     2881 ctttttgaaa tgctttcagc aattgccaat gttgttgttc cagactcaaa ttctttgact
     2941 gcaccatctg gaaaagtaat ttttatcata atagtttcct ccttgaaatg ttttgattga
     3001 tcagatcatc aagctgaaaa acaaaaaaat cctagcatct cttgaaaaga gatactagga
     3061 cgaaaagttc gtggttccac ctaatgttca aacgcaaatg cgttcctcaa gcgttgtaac
     3121 ggtccgaccg cccttgcttc aacaagggtt tcgaaagtgg tcatcctttg gttttctcct
     3181 ggaaaagttt cagcctagct tttcctctct tatcgaagac tctcaaattc agttggcttt
     3241 ctcatcaatc aattatgtgt ttatttaacc actaataaat agaaaaatca agtgggaaac
     3301 taaacttatt ttttccattc cccatttaca ccgtatgttc cttttttcat ctcattgatg
     3361 atgacatgga tgtgttcttt tggtgcctgc gtgtttttat gaacagcttc cgtgatatct
     3421 ttcatcatat tttctaattg ttccggactg cgaccttcta ctaattcgac atgtacgaat
     3481 ggcataatgc tctctcctct ttctgatcgt tcttttttca actcgattct atcatactat
     3541 gtttgtcttg ggtactgaat caggaaaaat ggctggatct ttttttgctc tcaaggcact
     3601 caaaatagag accgtatcca cgacttcttg caaaacagct ccgaataatg cggggattag
     3661 tcccgtactt gcaattaaca tcaaaaaaat acaaataaag atgccaatca gtacagactg
     3721 tttcgctatt ctcatcgtat ctcttgaaat cactactgct tttgctacac gcaagagatc
     3781 atctttcaag atgaccgcat ctgcactttc actagcagct gtagaaccat gagcccccat
     3841 tgcaataccg atatctgcag cagcaagtgc tggtgcgtca ttcacgccgt ctcctaccat
     3901 cgcaacaggt ctttgatctt tagggatact ttctaaaatg gtcagtttat cttgaggcaa
     3961 gctacgggca tgtacagaat ctaattggac tttttctgca atcgtttgag caatggcaga
     4021 tccatccccg gtgatcatca tcaaattttt cacatcaagt tttcttagct ccttgattgt
     4081 ctttgctgct tctgggcgaa tctgatccga aaataggatc gttcctgcaa aagatcgatc
     4141 aattgttaca tagattgctg tattggacgt ttctatttct tcagctgaaa caaaagaggc
     4201 acgtccaatc tttacttcat gattattgac tactgcttgg attccttgac cggtcacttc
     4261 tttcagttga ctaacaggat acagttggct gtttttcacg ttgttcacta acgaacgagc
     4321 taagatatgg ctagattcct gttctgcact agctgcatac agaagtagct cttctttttt
     4381 tattccattg actggctgaa tcgcctctac ttccaatatc cctttggtaa gcgttcctgt
     4441 tttatcaaaa gcaatcgttt ttgtacgtgc gagcttttca atcgttgttc cagtttttac
     4501 aacgatcccg tttttacttg aacggctcat tcctgcaact aaagcaatgg gcgcagctag
     4561 aatcagtgga caaggtgatg ccactaccag tacctcagca aatcgtaaag ggtcttttga
     4621 gaaccaccaa gaaataccac cgatcaaata agcaatcaac gtgaaaggga ctgcataacg
     4681 atcagctaaa cggacaaatc gggcaggctt ttcttttgat tcttcgacta gtttaataat
     4741 catttgatac tggctgtctg cggcacgttt ttcagcgatc atttggatcg atccgtctcc
     4801 atttaaagac cctgacataa tctgatctcc aggtttcttg gtttccgggc gggactcacc
     4861 tgtcaaagaa gattcatcga ccgaagattc tcctttggtc actcgaccat ctgcgggtac
     4921 aagttcgcca ggcttgacta gtaactggtc atcaatattt atttcatcga ttactatatc
     4981 ctgcaatttt ccttctttga atcgatgggc aattcttggg gtgttatcta acaaagactg
     5041 cagttcttta cccgctcgtt tattcgcgta atcctctaga ctatctccgc ccgtaagcat
     5101 gatcaatatc atcaaactag cccagtattc tttgacggct aatgttgcaa ggattgcggt
     5161 aatcgctaat acatctacgc catatcttcc tgaacgtaac gtcttgatca tttccaaaaa
     5221 catcaagaag gcgagaatcc ctccagtaaa tgcgacaatc gcaaatgcaa gggccggctg
     5281 gtgaaaaaca aattcactta ataaagctat cccaccaata agcaaagtaa gtaaaaattt
     5341 tttgaaatgt gtcattgttt tttctcctct tttatgtgac ttagagaggt tagtaataaa
     5401 tttccagctg cctctttcat cttaagcata gtccttgact agcacttttt aaaacaatag
     5461 gccttaattg aaaacaacta tcactatcag cgtttccaaa attcatcagt tctacaaaca
     5521 aaaatattca gcaaggatta ctttttcgat tcatcagtta aaaagtacct tttttcgaaa
     5581 aactatgcta tacttttaat tcgttattta cggcggaagg agcaaacatg cgatgaatga
     5641 gaaacaacta gaacaaacgc acgtggatga aacgatcgaa ctaatccaga tcgaacaaaa
     5701 tatgctggat acaaaacaac aagaattgac tcaggaaatg aatcattcga caaaagacac
     5761 agctaatcac aagattcgag ctggttccaa tgaagctttt tatgaatctg ctgttgaata
     5821 tcgccaacat gagcaggaat tgcttttgaa ataccatact cttgaagcac agcaaaaacg
     5881 cctgcaaacg ttgtctgtca tgaaagatag tccttatttt gcccgtatcg actttaaaga
     5941 agaccgtgaa acagaaacat tatatctagg tatcgcttct ttaagagatc tggcagaaga
     6001 aacaatcgtt attgactggc gcgcaccaat tgccaatctt tattatgaag gtgagattgg
     6061 tcccgcattt tatgaaactg aaacagatag aatagatgtc gatctgcagt tgaaacgaca
     6121 atttaaaatc caagaaggac gtattttatc tatggtagat acgtcggaag taatcaatga
     6181 tgactttttg ctagagatat tggatgaatc atcaagcaac catatgaaaa acatcgtctc
     6241 tacgatacaa aaagcacaaa atgcaatcat tcgggacaca acaagtaaag taatgctgat
     6301 tgaaggaatc gccggaagtg gaaagacatc cgctttgctt caacgtattg cttttctgtt
     6361 gtatcataat cgcaagtggc tcgaggctga aaacgttctt ctattttcac ccaaccattt
     6421 gttttctgat tacatctcga ctgtattgcc ttcattaggt gaaagcggcg tgcctacaca
     6481 gacattcaaa aattatttgg tgcagctgct gccagagttt tctttactag atgaacagca
     6541 acaagaaagt ggttttttgc taggggaaaa agatccgata caagtgatga aaagcggatt
     6601 gaccttagtc gatcagattg atcgctatat ccaaggcatc acaaactacg gtcccttgtt
     6661 ccgtgatatg aaaataaatg gccgtacgat cctatcaaag gaatcgattc gccaatggta
     6721 taaagaaaca aatgaacaat tacctttaca tcatagactt tctctattac aaacgaagct
     6781 cctgaaaaaa ttaggcggtc tgcaaaaaga cgaaacacgt cagcaatggg tcaaagatct
     6841 ggcggaagaa cagctccaag aactatacgc aacagatcca aatcttgaat atacagaaca
     6901 aaaggagcgt tcattacgta aaaaactggc caaccaaatc gtacgtaaaa gattccgtcg
     6961 aatccatcga gggatcatga attatcaatt tgtcaatata acgaaacaat atctccactt
     7021 tctacaaact gtgccacttt ctcttctaac acgacataaa atcagcgaga ccaggtggca
     7081 ggaacatttg acaactgttc gtacacactt gcgtcaaaga gaattgcgtc aagaggatgc
     7141 tgtactattc tttttactga tgcgccgaac atatccagtg acggttacac acaaagctcg
     7201 ctatatcttt attgatgaaa tgcaggattt cgcaccatca caagtggcat tgctgcaagc
     7261 cttgttccca aaagcaaatt tgactttctg tggagatcta aatcaaaatg tctttggaaa
     7321 tgaaacgatc gttggatcat tggaagacct tttcgtggac cgtgaagtca ctcgtttcca
     7381 attaacgaca agctatcgct ccacaaaaga aatcactgat tttgctaatc attttattgt
     7441 gaaagaacat caagtagaaa caacagcaag aaaaggagac cttcctttgc tcatccaagg
     7501 aacaacagat gatatcaaat tgaattggtt agcagatacc attcgaacga ctcagggaaa
     7561 agcccggtat tggcgtacag caatcatagc taaaacacaa gaagaatgcg aagctcttta
     7621 tgctcaactg ccagacgagt tgaaacaaca agtccagctg attttaaatg agagtgactt
     7681 tatgaagcgt tccattattt tacttccagc ctttttagca aaaggtctgg agtttgaccg
     7741 agttttcttg tgggatgtag atgcagaaca tttttcttct cagcaagaca aattgatcct
     7801 ttacaccatg tgtacacgtg cgatgcatga attgaccctt tttacctcca ataatgaaag
     7861 tcctttgtta aacaatatgg atatttcatc ttatgagaaa aaagtactgt aagtaaaaag
     7921 acaactgcta tcattaatgt tagcagttgt ctttttattt acaataataa ttgcagaaaa
     7981 agtaattcaa tcatttaagg tcatttattc tatatataaa tccaaatctg tttcaggata
     8041 ttttaatttg acaagcgaaa taatcatttt cagttcttcc atgttttctg gatattctgg
     8101 gtcaaatacc ttgctatgac ggccaatccc gccattgtcg atcaatcggt aaattcgatt
     8161 ccattcaatg aattggtcaa ttgattggaa attgctgatt ggatagccgc cacaatccaa
     8221 aatctctgat acacgaaatg ctaggtatgc actatcgatc actttatttt ttttcgtgtc
     8281 tatcttctca tttctttcga taagaatttt ttcaggacga tgtttcaagc tccactctaa
     8341 tagtagaaag aaagcctcca tccgatactc ttcttcaaat aaccgaaagg ctgaatcaga
     8401 acgacgaaaa agcagcggaa tcagataatt ttgtctataa atcagcattg gtgcgcggtc
     8461 caccatacac tctaaataac ggttcagata ccctgtaccg acatagtttc ctaatcgata
     8521 gccttcaacg ccgaatcttg cgtatttttg cgctaatttt gaacaatcag ttacgttcac
     8581 agctaactct tctactttgc ggtaaccgct taagatacgt tcaaaaggtt ctaaagtgat
     8641 ttttgcttcc atattcttcc ccgtccatct tccacatact tcatatcatt tcacatgata
     8701 cttatgctct catcatacca caggttgaat tagaaaagaa aataaatcac acaaaaaagc
     8761 aagacaatct gttagtgaga ttgtcttgct tttcgaaaaa gactgaatac ttttcgttaa
     8821 aactggttgc ttagtcttct aatgccacat tatggaacac atgctgtaca tcttctagat
     8881 cttccagtgc atcaatcatt ttttcaaatg tttcttgatc ttctggagat agtgttacct
     8941 cgttttgagg gatcatttgg atctcagcga ttgaaaaatc agtgattcct ttttctttta
     9001 atgcttcttg tatactatgg aaatccgcta cttcaccgta aacgatgatt tgtccttctt
     9061 cttccattac atcgcgaaca tcgatgtctt tttccattag gtattcaagg atctcgtcag
     9121 cgtcttctcc tgcaaaagca aagattgctg tattgtcaaa catgtaagaa accgctccgg
     9181 aaacacccat gttcccgcca tttttcccaa atgctgcccg tacgtctgct gctgttcgat
     9241 tcacattgtt cgtcaatgta tcaacaatca ccattgagcc gcttggtcca aatccttcat
     9301 agcgcaattc agagtatgtt tcatctcctg caccttttgc tttttcgatt gctcggtcaa
     9361 taatatgttt tggtacatta taagttttag cgcgatcaat gacgaaacgt agtttttggt
     9421 ttgcatgcgg atcaggatca cctgattttg ctgccgcgta gatttcaatc ccaaattttg
     9481 cataaatccg gctattatta gcatctttag cagttttctt tgctacgata tttgcccatt
     9541 tacgtcccat attttcactt cgctttctga tttgatagtt tcataacgca aaaacgtcct
     9601 gttccattat acttgtgaat cacgattctg tgaagctttt tttctttctt ccttcattct
     9661 tctcattttt agtcaattat ctatactcgg attgggattt gcccgaaatt ctgttccgga
     9721 atattgcctc ccaattgata gatttgatca cttctaacta acagttccgc ttcttgtgcc
     9781 tctttttttc cgattcggga ataaatagaa atctcttttt tttcttttaa gaatcttctt
     9841 ccagcatcgg taaatccaag tagtcgaaga taatttttat tttgttggac aggaatctct
     9901 tgctttttga tattcagcaa gacataacaa gataatcgtt ggatccgtgt ccatgtatat
     9961 ctttttgttt ttacctttga cacaaaatcc tcaaaactat cagcagttgc agctgctgtt
    10021 ttcatgcgcg attctattcc ttcggtcatc tgatagatat ctgtcagttc cagcaaagta
    10081 gaagataaca accgataacg caaatacggc caataagatt cccaagtctg cagcgaacaa
    10141 gttgaaagat cttcttctgt ctgaggcggc acaagtccag ctattctttt tccttcttct
    10201 aacgcatggc gaatggctgt tgcactggct atcctctgat ctgtgatgat tttttcatga
    10261 tagcctgcac ttttacgtcg gatcggatgt aattccatag ggtggacata cttggcattt
    10321 tcctttgcat aacttaatcc caagatatga tttggcgaag aaaaatcaag cgtaatatcg
    10381 ggatacagac tgcgaaaaac ctcagtcatt ttttgcggat aactatacgt ttgatcagac
    10441 agttcttgaa atgcctcttc tatcctatct tgattctctt ggacaaagcg tccgaaactg
    10501 ctgtaatcaa acgaagtctc ttcttctgtt ccaaaacaca aactgtcaca gtccagtgtt
    10561 tgcaagattt ttactgcacc tttagcaaaa tagtcagctg actgaaccgc ccattcgacg
    10621 ggaagctcca ctactaaatc cacaccattt tgtaaagctt cattcgcgcg atgccattta
    10681 tcgatgatag ccggttcgcc tctttgcaaa aaattcccgc tcattacagc aatgacgacc
    10741 tctgctcctg ttatttttcg tgccatttcc acatgatagc gatgtccatt gtgaaaagga
    10801 ttgtactcta cgataatccc gcagcttttc atgcgcatcc tccttcatag ttatcgataa
    10861 aaaagaaata gagacaagta aaaaaaggca aaaatcggaa aaaatacccg aactatataa
    10921 actattctcg caaacacaat acttctgcga cgaaaagctg cactgcaaga gcaatgccta
    10981 tatcgttcgg atatataatt tatgttgaag atttattctt ttcttgttaa ctcgagcagg
    11041 caaaaccgct agtctttgta tgctacaaag aaccagcgct ttgatttctc tgatgctgct
    11101 tcgtctgtaa aatcagaaaa tgcttcgact ttcgaaaagc cgctgttttc cagcattctt
    11161 aaataacttt ccaatggata ggtacgctct tgatgtagtt catcttcacg aataaatcga
    11221 ccagaccctt cttgttcttc aacgaaaaag gtaagaaaat gttcgatgct gtgtttctcg
    11281 cttcctggat aactatccca caaaaaagca aaattatccc cttgataatg atagctatat
    11341 tctggaaaaa cctcatctac ttgataggtg gagtgtacgt caaataaaaa tcggccattt
    11401 tcttctaacg cttgatagac ttcatcaaac acttgctgta cctcttgtct atttttcata
    11461 tagcacagcg aatcagaaaa acaggtaatt gcctgatatt gtccgatttc tgataaatcg
    11521 agcatattgc cttccacaaa ttggatcgat gcgtcttctt catatgctcg ctggctggca
    11581 atcatcaaca tctcttctga aaggtctaac cctgtcactt tatatccatc ttttgcaaag
    11641 gcaaccgcta atgctccagt accgcatgcc agttccaaaa tattttttgt gtcttcttgt
    11701 aaatgacgtt tcgaaaaagc taaccattgc tgataaagtg tttcgtccat cacttcatcg
    11761 taaatgaaag caaatgtttc ataagccata tgttaatcca accattcact aagatcgact
    11821 agcggagcat ctgaccatag tttttccaga ttatagaaat ttcgttctgg cgcatggaaa
    11881 acatgtacga tcacatctcc aagatcgatc aatacccatt ttccgccttc ttttccttca
    11941 atacgtttta cttcatattt gttttcttct tctttatcaa tgatttcttc tgtaattgcg
    12001 ttgatttgac gttcgctatt tgctgaacag atcatgaaat aatctgccaa caaggaaaca
    12061 tttcgaacgt ctaaagcgat aatgtcttct gcccgttttg aatctgctgc ttcaatcgca
    12121 atttttaatt gttctttact atctatgatg atttcctcct aatatgagca gcttattttt
    12181 ttgctaccca gtaattatat gtttcgattg tttttggata aatctgttgt tcttgcgcaa
    12241 gcaaatgggc caatgtatgt tttgtttcaa atgctacagc ttcgtctaga tcagcccatg
    12301 caatggctcg tgcatcttgt accccaggaa aatctcgacc tggttcaata tagtctgcta
    12361 cataaatgat tttgtcaagg agactcatct tagcagcccc agtcgtatgc acccggattg
    12421 catgaagaat ctctgcatcg gtgattccta attcccgctc tacaaacgaa gcgccaacaa
    12481 gaccatgcca gattgcatta ttataattca ataactccgg atcaaaaccg tctcttacga
    12541 ttaccatttt gaactcctca tctggtcgtt cttttgcata atcatgtgtc aatgcagcaa
    12601 tactagcttt ttcaggagaa gcaccgtatt ttttggcaag agccacggct gtttcttcta
    12661 ctccaagcac atgcttgaag cgttgttcgc tcattctcat ctgtattttt tgcatcaggt
    12721 tttctctaga ataagcagta tattcattac tatagtccat attcatacaa cccttcattt
    12781 tgaatataat caattacttt atctggaaca aggtagcgga tagaacagcc ttctttgatc
    12841 ttttgacgta ttttcgtgga actgatgtcg atctcaggta catctaccca aattacagga
    12901 tatggagtat ccgttgtgta tcctggtcga cgtataccca caaagttgac catagaagtc
    12961 agctcatcga ttttatacca ttttggcaaa tattctacca tatccccacc gataataaag
    13021 taataatctg tatccggatt gttttgcgtc aattccttca tagtatcata ggtgtaactt
    13081 ttcccaccac gagctaattc aatcgtttca atctgcaaaa aaggattgtc ttccaccgct
    13141 aattccaaca tgttgagtcg atgttttgca tcaatcgtct gtttctcatc aacatgaggt
    13201 ggctgataag aaggcatcaa aaatacgcgg tcaagaccta aattccggcc agcttgttca
    13261 gccatcacta aatgtgcaag gtgaacggga ttgaatgttc cgcccaagat ccctacttgc
    13321 tttttcttct ctaagaattg aggcatttct tcaggaaata attgtgaacg aacaaatgtc
    13381 ttagcttgcg tattcatcat ggtcactcct tatattgctt ttacttcttt tgatagcctt
    13441 tgatactttt cttttgtgga aggtttgtat aatacaataa cacgtccaat gatttggacg
    13501 atttcgcaat ctatttcttt ttctaatact tgagctgctt cttctgcaat ctcatctgta
    13561 ttttgaagta acgatacttt gatcagttct cttttttcta aagcttcttc aatttgaacg
    13621 atcattgctg aattgatccc accttttccg atttgaaaaa tcggctgaag atgatgcgct
    13681 ttgctacgta aaaaacgctt ttgttttcct cttaaactca taattttcct tcttccttaa
    13741 atcaatgctt tgcgtttcaa tacatctaca ccctttggcg cccagcccgc aattactact
    13801 ggcgctgtta cagtgatcca gcctagaccg gcaaaaacga tgtctgtttt ttctttcaca
    13861 gaaaactcaa aacgaacaag ctccggaaat gcctcgactt catcaggacg aggaggctgt
    13921 aataatccac ctacatgttt ttgataaaat tcgtctgctt tttccagttt cgttcgatga
    13981 atatttacat catttgacac ataagcaata aatgaagcac gattgccgct gataaaatca
    14041 aatcttgcaa gtccgcccaa gaaaagtgtt tgcccttcat tcagctgata aactttaggt
    14101 ttgatttctt ttaacggtgc aatgatccgc aaatcttttt tacctaagta gtgcgccatt
    14161 tgatggcgat ggataattcc cggtgtatca atcaaaaaat gcccgtcatc taaaggaatc
    14221 tcgattttat ccaaggtcgt tcctggaaac tgcgaagttg tgattacatc ttgtactccc
    14281 gctgtatttt tgatgatttg attgataagc gtcgattttc cgacatttgt tactccgacg
    14341 atatagacat cccgtccatc gcggtattct tcgatttttt ctaatagttc cgtcatctca
    14401 tgagatttct tagcactagt taataataca tctactggac gaattcctgc ttcgtaagca
    14461 cgttccttca tccattgtgt taatttagtt tttttcaatg atttaggtaa tatatccact
    14521 ttgttcccaa ctaaaagtac tggattgtca ccgacaaaac ggtgtagtcc tgggattagt
    14581 gaaccattga agtcaaaaat atccaccaca ttgacgatca atgcatcttt ttgccctaat
    14641 tcattcagta agcgcaaaaa gtcgtcatcc gtcaatgaga catcttgggt ctcattataa
    14701 tggcgtaatc tgaaacaacg ctgacaatag acttccccgg aagctaaacc tttttccaaa
    14761 gccgtttgcg gcgtgtatcc taattcaccg ggtttgtttg tttgtataac ggcaccacag
    14821 ccgatacatt gtaattcttc tgtcattcga ttccacctcg ccagcccata tcagggtgtt
    14881 ttgctaatag ataggccata attctttttt cccaaaaacg attaaattgt gtcttccaac
    14941 tatctgttgc gataatgggt tttacaagga tgctacgtat ccctgcacga ttggctccac
    15001 gtatatcagt cataatctga tcgcctacca ttactatctc atcatctgct aaatccaatt
    15061 gtttctttgc tatgttgatc cctcttgcta aaggtttcat tgctcgtgca acatacaaaa
    15121 gatcaaattt ttcaatcgct cgcgcgacac gttttgaatt attgtttgaa acaacaacaa
    15181 cagggattcc tgcatttttc atttctaaaa tccaagtaag caactcttct gttccatctg
    15241 gattattcca tgcaatcaac gtattgtcta aatcggtcaa tactgctttg atgcctagat
    15301 tctttaattg ggcaggtgta atctgataga tggcttctac catccatgtt ggtttgtaat
    15361 ttgaaaacat gctgtctcct catctgcaaa aaatgaagga gcacataaag tactcctcaa
    15421 ccccgtatta tactgcatat cagcaaaaaa aactacctct atactcgttc gtctttcaga
    15481 aaaagcattt ctttacaaaa tgaccaaaaa taaagattga tcacagaaaa atttagaaac
    15541 aaaaaacaac taatattggc ggaacctctt gtatcgtttt tccagtaatt tatgctgatc
    15601 taatcgaaca agttccgtca atctttcaat caacgctttt ttcataatgt gtgcgacttt
    15661 atatcgtggc aactgctcgc cgttgaacgt ttcaggaata actcgatcaa cgattcctaa
    15721 ctcgcgtagt tctggcgcag taattttcat caactcagct gcttctgcgg cgcgactacc
    15781 gtctttccac aaaatcgacg caaatccttc tggagaaagc acagcataaa tcgtttcttc
    15841 cagcatccag acttcatccg ctaaacctag agcaagggca ccgccacttc caccttcacc
    15901 aatgatgatg gcgatgatcg gtacctttaa atcagacatt tcgagcagat ttctggcaat
    15961 agcctctcct tcgccgcgct cttctgctcc tacaccgcaa tatgcacctg ctgtattgat
    16021 caaggtaatc accggtctat tgaacttttc cgcttgtttc atcagtcgta aagcttttcg
    16081 atagccttca ggatggggag aaccaaaatt tctgcggata ttttcctgca gatcccgtcc
    16141 tttttgaata ccgatcactg ttaccggttt gccatctaat cgagcgattc caccaacaat
    16201 cgcttcgtca tctgcaaata aacgatcacc atggcattcc tcgaaagaat cgaagatctc
    16261 ttcaaaaaaa tccaagcttg tccatcgttc tttagctctt gctaactgaa cgacatcatg
    16321 tgctgtctta tccacgatct atccatcctt tctgtgtatg caattcaaga agcgtatgga
    16381 ttttctgttt caattccatg cggggaacaa tctgatcgac aaaaccatga gataataaga
    16441 attcagcctt ttggaaatct tctggaagtt cctgcttgat cgtctgttcg atgacgcgtc
    16501 ttccagcaaa accgatcaag ctttggggtt ctgctagaat aatatcgcct tccattgcaa
    16561 aactggcagt tactcctccg gtcgtcgggt ccgttaaaac agttagataa aaaagacctt
    16621 cattgctatg ccgtttgaca gcagcagaga tttttgccat ctgcattaga gaaaagattc
    16681 cttcttgcat ccgtgcgcca cctgaagctg tgaacaagac gactggaaga ctttcctttg
    16741 ttgctcgttc gaataatctg gtgatctttt ctccgacaat cgttcccata cttcccatga
    16801 taaaattagg atccatcact ccaataatga agggaatccc ctgaatggtt gctttaccag
    16861 tgaggactgc ttcatgaagt cctgttttat cttgcatttt tttaattttt tccgtatatt
    16921 cgggaaatgc tagcggatct ttggttacaa gatccgtatc ccattcttca aaactttttt
    16981 catcaataat caaagctaga cgctgccatg caccgattcg aaaagcgtat ccgcaatgag
    17041 ggcatacttt ttcttcaccg atgtctttcg tataaaggat atgtttgcaa tttggacact
    17101 ttgcccacat gttatcagga acagatggcg agttagcagg tttgctctct ctattaggat
    17161 tgatccgaat atatttcttt ttcttaaaca gagccatcta tgcgcctcct atctccgttt
    17221 ctggtgtcca attcggtaaa aattcttctt gtaaaaatgc tgtgctgtaa tcacctgcta
    17281 tgactgcagg atgactaatc aaatccatct gaaattctgc gttcgtgatg attccttctg
    17341 tgataaattc acttaaggct cgctgcatct tcattaatgc ttcaaaacgc gtatttccat
    17401 ggacgattat ttttgcaatc attgaatcat aatatggcgg gatcgtatat cccgggtaaa
    17461 ctgcactgtc tactcgcagt cccatcccac ctgctggaag cagtaagttt tggatcttcc
    17521 ctggagaagg agcaaaatgg aaagcaggat tttctgcatt aatccggcat tcgattgcgt
    17581 gtccttggaa tactacttct tcttgacaca cattcaaagg ctcaccggct gctatctcca
    17641 cctgtttttt tactaaatcg attcctgtga ccatttctgt tactggatgt tccacttgga
    17701 ttcgagtatt catttccatg aaataaaaat cgccttcttc atccatcaga aactcgatcg
    17761 ttccggcatt ttcataattg actgctttag ctgctcgaac agcagcgtct cctagtgctt
    17821 ctctttttgt ttgtgagatc actacagaag gagattcttc cagtactttt tgattgttgc
    17881 gttgtaaaga acaatctcgc tcgcctaaat gaatgacatg accaaatcga tcacctaaga
    17941 tctgaacttc tatatggcga gcaggataaa tgattttttc caaatacata tcatcatttc
    18001 caaatgccgc tgctgcttct tgctgagctg aagaaaaatg ctgtgggagc tcttcttttg
    18061 attgaacttt ccgaatgcct ttcccgccac cacctgcagc tgctttcagc atcactggat
    18121 aaccaattct gtctgctatt tccaatgctt cttctactgt agataataca ccagtacttc
    18181 ctggaatgac tgggacattt gcttcttgca tcaattgacg cgcattgatc ttatttccca
    18241 ttgcatcgat cgttgcactt ttaggaccaa taaatgtgat attgcactct tcacacatgg
    18301 aggcaaatcg gctattttca gataaaaaac caaatcctgg atgaattgct tcggcttttg
    18361 tcacgattgc agcgctcagt acttcctgaa cattcagata tgaatcaacg gcttttgccg
    18421 ggccaatgca gattgcttca tctgctaact gtgtatgaag tgcttcggca tctgcttctg
    18481 aatatatcgc tacggtttgg atccctagtt cgcggcatgc acgaatgata cgtacagcga
    18541 tttcaccgcg gttagctatc aatactttag aaaacatatt tcacctatcc aatcataaag
    18601 gttaattccg cttcggctac ttttttaccg tctactttag cgattccttt gcctattccg
    18661 gctgaagctt tcactttcaa tatctcaact tcaaggtata aggtatctcc cggtgtaact
    18721 ttcttacgga attttgcttt gtccaatccg ccgaaataag ccgtctttcc cttgaattgt
    18781 tcaagagaaa gtaatgcaac tgctcctgct tgagccattg cttcaacaat caatacccct
    18841 ggcataacag gttcttctgg aaaatgtcct tgaaaaaagg gctcattgat cgttacattt
    18901 ttcttagcaa cgatccgttc tccttcaacc atttcttcca ctcgatcaat caaaagcatt
    18961 ggataacgat gtggaatgat ttcttttatt tcttgtatgt tcattgtttt ccctctttcc
    19021 tgctttgctt atttttgctg aatcctaaag agtggctgtc catattcgac aactgactcg
    19081 ttttctacaa ggatttctgt gatcactccg tcggcctcac tagttatctc attcatcagt
    19141 ttcatcgctt caataatgca gataacttct cctttggcaa cttgatcacc tacttttttg
    19201 aaagctggtt tgtcgggcgc tggttgcaga taaacgattc ccacgattgg cgaaacaacc
    19261 tcgattccat caaatactgc cgattctttt tctttcatta tttctggagc agttgttgct
    19321 tgttcaggta tgacattgac tggctccgaa actggcaacg tttcgtttgt ttgaaccata
    19381 ttcatgcctc gacttgtttt atttttattc atatacaatt caaattgacc ttcacgcaaa
    19441 tcgaattccg ttagcgagga ttgatcaaat tgactgacta attctctgat ttcattgata
    19501 tccaaaatta ttcctcccag cgtttcatgc acagaaccgc attatgtccg ccaaacccta
    19561 atgaattact cattgcataa gtaaaacttg caggtttgct ttcgtttttg acaactgtca
    19621 agtcaatctc aggatcttgt ttttctacat tgatcgtagg tgggataaat tgatgttcta
    19681 atgccagcaa agttgccaag gcttcgatcc cgccggctgc tcccaataaa tgtccagtca
    19741 tcgatttcgt gctgctgacg taagtatctc tgaaattttc acctaatgcg atttggattg
    19801 cttttgcttc tgcctggtca ttggctggtg tacttgttcc gtgagcattg atatatccga
    19861 cttgttccgg ctgtacacca gcttcttcca tcgcaagctt cattgcttta gatgcaccag
    19921 aaccatctgg agtaggagct gtcatatggt aagcgtcaca gtttgcaccg tatccaacga
    19981 tttctccata gattttggca ccgcgagctt gtgcatgttc taaagattca agaactagaa
    20041 cgcctgctcc ttcacccatc acaaaaccat tccgattttc gtcaaacgga atcgaaccac
    20101 gattaggatc agttgattct gagagtgctg ttaaagctgc aaatccagca atccccatct
    20161 cacaaatcgt tgcttctgtt cctccagcaa gaatgacatc agaatagcca tgtttgatgt
    20221 tacggaatgc ttccccaatt gaattcgttc cgctagcaca agctgttacg atagctgtac
    20281 aaataccttt tgcacctaca cgcaaagcaa tatttccagc tgccatgttg gcaatcgcca
    20341 ttggtacgaa cattggagaa actcgttttg cacctttttc atgtagacga ataacttggt
    20401 tttcgattgt tggaagacca ccgatcccag atccgatgat gacgccaaac cggtcttgat
    20461 tcattttttc tttatctaaa ccgctcatct ccattgcttc taaagcagcg tagattccat
    20521 agatcgaaaa catatccatt cgtttgctat cttttttaat aaaatattta tcgtaaggga
    20581 aatctttgac ttcacccgct aaggtaatcc ctgtttctga agcatcaaat ttagtgatcg
    20641 gtgcgatgcc attcgtcccc tcttttaagc tattcaaaaa gctgtctgcg tcatttccaa
    20701 taggtgaagt gactccatag ccagtaatta ccactcgatt catttgtttg ctctcctttt
    20761 cttatccgtg catgaccaat ccgccatcga cattgatgac ttgtccggtg atatatggat
    20821 tttgtgctaa gaaaactgct gtttgtgcaa tatcttctac ttgtccgaaa cgctgcatcg
    20881 gaatttgctt ttccgcttgt tccttgactt tgtctgctaa aacttccgtc atatcagttg
    20941 tgatgaatcc tggcgcaatg gcattacatg taatgcctct agttgcagct tctcgtgcca
    21001 cagattttgt taatcctact acgccagctt tactagctgc ataatttgct tgaccaatat
    21061 ttccaatcaa cccagataca ctagagagat taatgatggc cccttctcgc tgcttcatca
    21121 tttttttcaa tacgtgctgc gtcatattga aggttccaat caaattgata tccagacatt
    21181 ttttgaaatc ttctgcatcc attcgcatca caagtttgtc atttgtgatt ccggcattgt
    21241 ttactaacac atggatcgat ccaagctttt cttctgcttc tttgatcatt tgtcccgctt
    21301 tctcataatc agagatgtca ccggaaactc ctacgcattt tacaccgaat gcttcgattt
    21361 cttctatctt ttcttttgga atctctccgc gaccattcaa aatgatattt gctccagctt
    21421 ttgcaaaagc taaggccatc gctttgccga ttcctcttgt actacctgtg ataaatacat
    21481 tttttccttt tacatccata acgttcctcc ttatagtatt gctattgttt catcaaatgt
    21541 ctgcttattc tcaacacgca gaactggtat tgttttatcg atttttttca taaatccgct
    21601 taatactttt ccggggccga cttcgatgac ttgttcgatg ccgattgttt ttaacttatg
    21661 gatactctca tagaaacgaa caggttgcat gacttgctgt tcaagtagtc ccgcgattgt
    21721 ttctttcttc attatttccg ttgttgtgtt gcttatgata ggaaaagaag gttcagagaa
    21781 ttgaatctga cttaggtcct tcgccagttt ttttgctgcc ggttctaaaa tagctgtatg
    21841 aaaaggcccg ctgacattta gagggatcat tcttttgaac cctttttctt ttaatagtaa
    21901 aacagcttcg tctactgctt tttcttcgcc accaatcacg atctgctgag gtgtattata
    21961 atttgcagga gatacgatgc cgtacttgct agcttcatga cagctttcct cgatcgtttc
    22021 aatcggcgca ttcatcactg caaccatttt cccgcttcca gctggggcag cttctgtcat
    22081 ataagcgcct ctttttgcta ctaacgcaac tgcttcagtg aaggatagcg cattactagc
    22141 tactaatgcc gaatattctc ctaaactcag tcctaaagct gcatctggga tgattccatg
    22201 ctccttcaat aagcgataat acgcgatgct gactgttaga atagctggct gtgtatattg
    22261 cgtctgatcc aaacgttcat tttctgtaaa gcaaagttca gccatatcgt agccgagaat
    22321 ctcacttgct tcatcaaatg tctgctttac aacggcttct tcatataaat cttttcccat
    22381 accttgatat tgtgcacctt ggccactaaa taagaatgct gttttcatgt ttttctccaa
    22441 tatatccaga atatatgttt gattcttcct attcaaaaag gccgagacat tcctatttca
    22501 ggaaatgtcc cagcccccaa cctaatttta atgattatgc agtttgtttt tctacgtaag
    22561 tcactaaatc ttgaaccgtt ttgattccgt cttctgtttc gattttcacg tcaaattcat
    22621 cttcaatttc attgatgatt tggaataaat ccaagctgtc agcttctaag tcttcttgga
    22681 tgtcagtagt caatttgatt tcttcagttt cttttcctaa ttcttctgcg atgatttctt
    22741 gaattttttc gaataccata ataatatatt cctccgatat tgtttgcttt ttatttttat
    22801 aatttaacag tctacaactc catgagaata cttccccatg ttaaacctcc gccatagcct
    22861 gtaaatatta ctttctgctg ttttcctaag gtgatctttc cactagaaac tgcttcatcc
    22921 aataagatag ggatgcttgc agcagatgta ttcccatatt tatccatatt ggtcaggaac
    22981 ttttcttgcg gcatctttac ttttcgggca attttctcaa taattctgag gtttgcctga
    23041 tgcaaaagca gataatcaac tggtttgtct ttcatgactt cttggatatt gtttgcaacg
    23101 tctcgtacgg caaaatcaaa aatatctctg ccagtcattt gcaaataata cgaaccttct
    23161 gaatcttctg aatagaacgg atttttattt tgctggtagc cagcggtcaa tgagctactt
    23221 ctggtaccgt cagctgctaa tttttctttc agcaattttt tctgtggatt cgcttctaag
    23281 agaatcccac ctgctccatc gccaaacaac acagcagtcg tacgatcaga ccaatcgatt
    23341 agcttagata gtgtttcacc accaatcacc agtcccttag ttgagccgca gcggatcatt
    23401 ttttcagcca tgcttaacgc ataaacaaat cctgagcacg cagcactgat atcaaaagcg
    23461 atagcatgag ttgctcccaa tttcccttgt acttgtgctg ctacagatgg tgtcgtgtaa
    23521 tcgggcgaca ttgttgctac taaaataaaa tccaaatctt tagggtttag atttttctgt
    23581 tttatcaatt tttccgccac taaaatgcat aaatcagaag tctcctgatt tgttacacat
    23641 cttcgttcac gaatacccgt ccttgacgaa atccattcat cagaggtatc catcatcttg
    23701 gaaagatcat tattagtaat aatcttttca ggaaggacgc tggctgttgc agtaatcatt
    23761 ccaaattgtt ccattaaata tcctcactta tagtcttgca agaagtcgtg caagtttttc
    23821 agtgcatcca gcaaggcatg ctgttcctcc gtagccattc cgtctaagat gttcttgacc
    23881 atttcacgat gaaaatgctg atggacccga aatagtaact ttcctttttt cgttagtccc
    23941 aactttacaa cgcggcggtc atcttcactg cggatacgtt cgacatagcc tttttttact
    24001 aacttgttaa tagcagtggt caaggtccca acagtaattg atagttcttt ggctacttct
    24061 gaagttgttt ttttcttgta catcccgatt gcctcgatcg tgtgcatctc tgtgattgac
    24121 agatctttga attgagattt tttcagctca gactcttcga ttgtcaaaat atcattgaag
    24181 acactaacaa gataatcatt tactgttttt aaatctggtt ccatgttcca ccgtccacta
    24241 attagtttga ttatcaaatc ttttgatgtt caaactatat cttaactttt ttcaaaatgc
    24301 aaccttaaaa gatcattttt ttactatatg agcttttttt aagattttac tcatttaaaa
    24361 caactgataa atcagcattt ataatttctt ttattagaaa aatcatctat ctcacatatt
    24421 ttgattatca aagtattttc tgtgaacaga actatagaat tccctaaaac ttgtccatta
    24481 tttcaaaatt tgttatagtc tttttattca aagcgtaaaa ggagatccta tgttaaaaga
    24541 aaaaatgaaa actctcccca tgctgccggg tgtctatctt atgaaagacc gagatggtaa
    24601 ggtcatctat gttggtaaag caaaacggct caaaaataga gtaagcagct attttcatca
    24661 gaataaacag cattcaaaaa aagtattacg tatgattcat catataacag attttgattt
    24721 cgtcgtagta gacacggaat tagatgcttt attgctcgag tgtcaattga tccaacatta
    24781 ccgtcctttt tataaccgac aaatgaatta tttcagtaat tataattatg tacatattac
    24841 caataaggga tttgtattaa cagatactcc tacggctcga acatacgggc cttttcgtct
    24901 ctataaaaaa atgccgtcta ttttgcgtat aatggaagaa acttatcaaa tgccttggtt
    24961 gtctgaaatt agtttgttag ctttgcgagt ccagcttcct gacctacagg aaatgacttt
    25021 tgaacagaaa aaaaaggaac tccaagggct gtttcaaggc cgaaataaaa agctactgac
    25081 ttacttgaaa aaaagacaac aacatttcat ctatcagttg aatttcgaaa aagccggtat
    25141 gcttcaaaaa gatatcgaat tagttactta ttttattaga cggatccagg aacaaaagca
    25201 gtttttaaga acaccatcgc tcaccttcag catgccatta gcagcagatg agagtcagaa
    25261 aaagcactac ctcgtttgtt atggtcaact tgcagaaaca atgattacct ctggtgacgt
    25321 ttctcctgac ttctattatg aaaagaaaga agctcattta tctttgaaac gtcaattgtc
    25381 aaaagaagaa atcgatcctg tacaaatctt gatcagttac cgcaaaaaat tagaaaaaga
    25441 acagtttgaa attgaactgt taaacaaaaa agaagctgaa aagcaactaa attagttgct
    25501 tctcggcttc ttttaggcat cttcaacttc aaatgtcaat tatcctaaaa tttattggat
    25561 taagtcatag atttccatcg ctacgatatc gatattatca aattcgtatt gctgatgagt
    25621 atctgtttca gttaattcaa atgtatcggt cgctttatca taagttactg tacacttctc
    25681 tacgccttct ttttcaaaac ggcgaacttg cagttcatta tctgctgact cgaccatagc
    25741 ctccagtcgt tttatgattg cgactaattg tgaatgtgtc atcggaggtc ctccttttct
    25801 tatttctgtt atattgtatc aaaagcatag cctacttgca ttgattttct ctaaaaaaac
    25861 tcgtttttta tgattttttt tctggtattt ttccttcagg ccaccaaagt ttgatcaaag
    25921 cttgggttcc gtcattctca aaacctttat ctgccaactc ttcatatagt ttcaatgctt
    25981 gggtagttgc tggcaataca agatccattt tttctgcttc ttctaatgca atctttaaat
    26041 ctttgataaa atgtttgaca aaaaaaccag gtgtgtaatc ttcttttaaa atacgaggag
    26101 aataattgct taaagaccag ttagctgcac ttccacctga aagcgtctca atcactttct
    26161 ttaaatcaag tccgtttttt tgtgcataga caagcatttc cgtcattcct gtcatcgtac
    26221 ctgcaatcat gatctgatta gccatttttg tatgctgacc tttcccagca gaaccatgca
    26281 atgtaaatgt cgtaccaaat tctttgaata aaggcagtac ttgatcataa acggcttctt
    26341 ctccaccgac catgatcgtc agtgtaccat tctttgctcc taagtcacca ccggataccg
    26401 gtgcatctaa cgcatctgcg cctttttctt ttgctgtttt tgcaattttt tctgccaggc
    26461 ttggtgtatt tgtagtcaaa tcaactaaaa tatgtcctga gatatctgca gaaaaaatcc
    26521 ccgactgtcc atagtagatt tcttctacat ctttggggta acctaccatt gtaaagagaa
    26581 tattactttg ttctgctacc gcttttggtg tatctgccca gatagctcct tctgttacta
    26641 aaggatcagc ttttgctttt gtccgattat agacagctac ttcgtgtcct gcttctagta
    26701 gatggcgtgc gacagcactt cccatgacgc ctgttccgat aaaaccaagt ttcatttttt
    26761 cgacctccta aaaatcaaaa atccttgctt ctatgctagt atacgctaga aacaaggatt
    26821 ttttcataaa aaatgtttga attttatctt ttaaatgaat ttaattttat tttgattcgt
    26881 tcaatcgagt cttcttcttc ttttcttcgc atctcttctt catgataaac aaccatgtct
    26941 tgataaaatt cgatcatagc ttcgccgaaa tgtgtagatg aaatatcata aagtttagct
    27001 tctaaaactt ttggatcttt aggaacttca ctggaaatat aatcaataag tgctggtgca
    27061 aagtcttcgt cggtttcaaa agttttccct aatgaaggat gatcgaacaa tcggttcaaa
    27121 taagcatttc cttccacgac acaaggtgtt ccggctgcca tcgcttctgt ataagtaagc
    27181 ccttgtgttt ctgaagtaga agcactgaca aaataatctg ccgctttata ataaagtgct
    27241 acatcattgt tgtccacttc tcctgtgaac tgcacaaatt cttctaactg cagttctgag
    27301 atcaattcct ctaactcttc tacataaggt ccttttccta cgataactaa tctggcgtta
    27361 ggataagcgg agatgacctg aggaaaccca ttaatcaagg cttggatatt tttctcataa
    27421 gaaatcctgc ttaaagaaag aatcatcaaa tggttttcct gaagacctag tgactccctt
    27481 aatttgctga tatcctgctg agtgatatca gaacgtttga atttttcaat gtcgatacca
    27541 gtaggaatca cgcgaagcgg tgctattaca ccatagctgc gtaatgtatc aataacacgt
    27601 tcacttggac aaactacacc tgtcgtatga tttgcaaata atctagacaa gtattttaca
    27661 tgggattgcc tgatcacttt tcctttaccg atataatgaa ggtagtcttc atacatcgta
    27721 tggtaagtat ggatcagagg tattttcaac ttcttagcta ccatcttccc aagtatccct
    27781 gctccgaatt ctgtatgggt atgaataaga tctagttcta gttctttggc aaccaaataa
    27841 gcataccaca tgcctctaac aacaactctt cgatccttga aagaaataaa tggtacactt
    27901 ggcattcgaa tcacatcttt ttccaactta tccgcattag gatcggtcgt tgtaaaaata
    27961 tagacttcat gcccatgtct ttctaattct tgttttaatg ttttgatcga tgtagcaact
    28021 ccgctgactt gcggaaagta ggtatcggta aaaaagccga ttttcatgtt tttcgcctcc
    28081 aaactagtcc ttgacctgat ttactttttt taatttgcta gagacttttt cattcagtac
    28141 actttcgtat acatttttaa gttcttcacc gattttattg atacttcgct cttttgctgt
    28201 ttgataaccg gcttgtgtcg tactcggtag ttcttgattg gtaatctgtt ggatcagtcg
    28261 gataaattct tcgtttgtct tccccatata gcagttcttg ctatcttcta accagccatt
    28321 atacactgga atatctcgaa caagtacgtt ttgtttgctt gctaaagctt ctaaaacaac
    28381 gatcccttct gtctcttcat aagaagggaa aaagaataga tctgcacctg aatacgcacc
    28441 ttctatgatc tctccacgaa tgtaaccagg aaacaggaca ttttcaggat gatcttcttt
    28501 tacaattttc cggatgttcc gaggaatcga gtacatggga acatgaccaa accaaataaa
    28561 tgtgtattcg ggcatcttct tagcaatttc tacgaaatca atgattcctt tacgttcgaa
    28621 aaacaaaccg acacaaatga ttactttttg atccggagac agtttgaaat attctctgaa
    28681 cttttgttct ttcattggat cagggtgata tttctctaag tcgatcccat tggaaatagc
    28741 ttgaatcggc acacggatac catagctttc caacaactgt tttgaataag gtgttggcgt
    28801 gatcagatag tccgcttttt ggtacaatcc taccagatat ttttttacga taggagaaag
    28861 ctggttcgat ccgataaagg aatttcggaa atcttcctct gtagaatgcg catgatagac
    28921 aacttttttt ccttctcttc tggctttatt gaccatatta tgactattga ttccgtatgt
    28981 attgatgtgc aggatgtcat aatcttcttt ttcatctagt gtataagaga taccgacttc
    29041 cgtcagagca cgtttttgat gatcaagcgc acgcccgatt ccagattttg caagcatctt
    29101 ttcactttca aaatataata ataccttcac cttacgtccc ccttcactta tatagtataa
    29161 catagcctac aaactgtcac tcaaacttaa gccctatttt tgtctattct tactgacaaa
    29221 aacgttttct taaaaaagaa caaaaaaact ggcgtagctc ttttacaagc cacaccagct
    29281 tttgatacgt tgtttatttt acatattcat gaacaagttc aacgacttct tccatcatat
    29341 cgcattcttt caatgcacga tcagcaagtt cagccatttt cgctgtgtcc aagcgtttca
    29401 ttaaactacg cgttttaaga atagatgtcg cactcattga gaactcatct agacccatac
    29461 ctactagcaa tggaacagct gtttgatctc cagccatttc tccacacata cctgcccatt
    29521 ttccttcagc gtgtgctgca tcaattacat ttttgatcaa acgtaaaatt gatgggttat
    29581 atggttggta caagtaagaa acgcgttcgt tcatacggtc tgctgccatt gtgtattgaa
    29641 tcaaatcgtt cgtacctaca gagaagaaat ctacttcttt tgcaaacttg tctgctaaga
    29701 ctgctgctgc tggaatctcg atcatgatgc cgacttggat cgtatcagat acttccttgc
    29761 cttcacttat caatttttgt ttttcttctt caaagatcgc ttttgctgcg cggaattctt
    29821 tgagtgtagc taccataggg aacatgatac gtaaattacc atgaacagat gcacgaagca
    29881 atgcacgcat ttgtgtacgg aacatttcgt cgccttgttc agataaactg atacgcaatg
    29941 cacgatatcc taagaatgga ttcatttcat gaggtaattg cagatatggt agttctttat
    30001 ctccaccgat gtccattgta cgaacaacta caggttttcc ttccatacct tctaatacag
    30061 ctttgtaagc ttcatattgg tcatcttctg ttgggaagtc tggagaatcc atatacagga
    30121 attcagtacg gtacagacca actgcttctc caccattatt atggacacca actagatctt
    30181 ttggtgttcc gatgtttgca gctaattcaa aatgcttgcc gtcagctgtg acagtttctg
    30241 cattttttaa tttttcccat tctgcttttt gtgctgcgta atctgcacct gctttttcaa
    30301 attcagcttt ttgttcatct gtaggatcga tgattacatc gccttcgatc ccgttgacag
    30361 ccaaaatatc gccttctttg actttagcag taatttcttt tgtcccaacg attgctggaa
    30421 tttctaaaga acgtgccatg atagcagaat gtgaagtacg tccaccaatg tctgttacaa
    30481 acgcttttac aaaattgcga tctaattgtg ctgtatcact tggagtcaag tcatgtgcaa
    30541 caacgaccac ttcttcgttg atcattgatg gatttggtaa agtaacgcct aataagtgcg
    30601 ctaaaatacg tttagcaacg tcacgaatat ctgcagcacg ctcttgcata taagcattat
    30661 cttccattgc ttcgaacata ccgatataca tatccgttac ttcttttagc gctgattcag
    30721 catttacgct gttgtcttta atattttgct tgatttgacc taccatttct ggatctgaaa
    30781 gaaccatcaa atgggcatca aatacttgcg cttctgcttc acctaagctt tgcgctgctt
    30841 tttcacgaat ttgctgcaat tcttcagtag atttagctaa tgcagcatcc aaacgagctt
    30901 cttcagccgc agtatcttct actgttgcct tactgaatgt taaatccggt tgaactagca
    30961 ggtaagcttt tgcaacggct acaccgtcac tagcggcaat cccttttagc atttcaacca
    31021 ttattcagat aagccttctt tcttcattgt ttcaacgatt gctgccattg catcagcttc
    31081 gtcagcacct tctgcagtaa tagtaacatc agaaccttga cctacaccta aagacataac
    31141 gcccatgatt gatttcaagt ttactgattt acctttatac tctaagttta catcagagtt
    31201 aaatttgctt gctgtttgta ctaatagtgt agctggacga gcgtggatcc cagtttctgc
    31261 tactacgtga aattctttct tttccatatt aatcggtctc ctttaagtga gtgttgtttt
    31321 tatgtgaggg ttttcttttt tagtaattca gagaatccta acaaatgcca ccctttcatc
    31381 tgtaaagatt aacatttttt ttcgttagat acaacagttt tcattcaatt tcagataaaa
    31441 gtattcaaat tattccggat tctttttcat ccttttcttc acattttata acaaacgtgt
    31501 ttatttcctt tttcttgtcg ctttctcttc tcctgacgtt tatcattctt tcctttgacc
    31561 ctctttgttt tcatatgaaa cgaattactt gcttcccttt ttatttttag tataataaga
    31621 gtcaaaggtc aggaatggtc aatagagatt tcaccttttc tacctcataa atctcttaaa
    31681 aaactagggg ttattctttt acctcatgaa aaacgagatg taaaatatgg atgtacccat
    31741 ttttgtgaaa ggacgtgaat gattcatgct ttgtcaaaac tgcggcaaga atgaggcaac
    31801 gattcacttg tatgccactg tcaatggtca gcggacacaa ctcgattact gtcagagctg
    31861 ttatcaaaaa atcaaaaatc aacaaaatgg aggtctgact aatatggccc aaaatgatcc
    31921 atttggattt ggcagtttgg acgatctttt ccgttccatg tctcgccaaa tgcaacagca
    31981 aggaaattat gagcaaacac caccaacaca atttggcgga aacaacaact tcaatggtgg
    32041 tcagccacct caaggcaata cagaaggttt attgggagaa tacggaatca atatcacaga
    32101 acaagcacga caaggtgata tcgatcctgt gatcggacgc gatgacgaaa tcaaacgagt
    32161 cattgaaatt ttaaatcgcc gaaccaagaa caatcctgtc ttgattggag aaccaggtgt
    32221 tggtaaaaca gcagttgttg aaggtttagc acaaaaaatc gtagatggtg atgtaccgca
    32281 aaaattaatg gacaaagaag tcattcgttt agatgtcgta tctttagtcc aaggtactgg
    32341 tatacgagga caatttgaag aacgtatgca aaaattgatt gaagagatcc gtcaagcaga
    32401 aaatgtgatc ctattcatag atgaagttca cgaaatcgtc ggtgctggtt ctgctggtga
    32461 tggaaatatg gatgctggta atattttaaa accagcttta gctcgaggag aattgcaaat
    32521 ggttggtgct actaccttaa atgagtaccg catcatcgaa aaagacgctg ctttagaacg
    32581 tcgtatgcaa cctgtacggg tagatgaacc aactgtcgaa gaaacgatca gtattttgaa
    32641 aggtttgcaa aaacgttatg aagactatca tcatgtaaaa tatactgatg aggccatcga
    32701 agcagccgca actttatcca atcgttacgt ccaagatcgc ttcttgccgg ataaagccat
    32761 cgatttgcta gatgaaacag gttctaagaa aaacttgacg atccaaatcg ttgatcctaa
    32821 aacaatcgag aaaaaactgc aagaagcaga agaacaaaaa gttttagctt ctcgagaaga
    32881 agatttcgaa aaagcagctt attatcgcga tcagatcaat aaacttcaaa aaatgaaaga
    32941 acgtcagctc actgaagagg aaacacctgt gattaccgaa aaagatatgg aaaaaatcgt
    33001 tgaacaacgg acaggtattc cagtgggtga attaaaagaa aaagaacaga cacaactgaa
    33061 aaacttagca gatgacttga aagctcatgt catcggacaa gataacgctg tagatcgagt
    33121 agcgaaagcg attcgccgaa accgtgttgg attaaacaaa cagaatcgtc caatcggctc
    33181 cttcttattt gttggaccaa ctggtgtagg taaaacagag cttgcgaaac aacttgccta
    33241 cgaactattc ggttcacaag attcaatgat tcgtttcgat atgtctgaat atatggaaaa
    33301 acatagtgtc tctaaattga ttggttctcc tccaggttat gtcggatacg aagaagctgg
    33361 tcaattaaca gaaaaagtac ggagaaatcc ttacagtctt gtcttgttag atgaagtaga
    33421 aaaagctcat ccagatgttt tgcatatgtt cttgcaaatc ttagatgatg gtcgattgac
    33481 agatgcacaa ggaagaacgg tcagctttaa agatacgatc atcattatga caagtaatgc
    33541 cggaactggt aaagtagaag caaacgttgg ttttggtgcg gcacgcgaag gtgttactcg
    33601 ttctgtattg aaccaattaa acaactactt tacaccagaa tttctgaacc gttttgacgg
    33661 gatcatcgaa ttcagtgcat tatcaaaaga aaacttgatg acaatcgtca cactaatgct
    33721 agacgatgtc aaccaaatgc tggcagcaca gcaacttcac atcgaagttc ctacgaatgt
    33781 caaagaaaaa ttagttgact taggctatga tccatctatg ggtgctcgtc ctcttcgacg
    33841 cacgatccaa gaacaaatcg aagatggtat cgctgaattt tatcttgatc acccttctat
    33901 ccatgagttg aaagcaaaac tggataaaga tggaaaaatc atcgttactt ctaaaccaga
    33961 acgtttggct aaagaatcag ccgaagaaac ggctgaataa atccagtttt atagcgtgag
    34021 acggtatttc tttgagaaat gccgtctcat tcttttttca gaaaagtcaa aatactgttc
    34081 attttataaa aaagaaatta taataagaga aacaagcaat taaaaataca taaaagaggt
    34141 gagcgccatg aaattggtaa atgtaacaaa cagccattcc cgtcttgtat taaaccagct
    34201 tgaaaacact gatgcccatc tagtcaaggt ctacacagcc ggaaatacca ctattgtcta
    34261 cactgaagcc cctgaacata atgaaattct gctgatcaat gacaaacgta aaatccagcc
    34321 gaaagaaatc gaagatgcta aaaattattt cttagataaa atcaaagacg ccgtttacca
    34381 cgaggatgaa atcaaagtaa tcgaattggc aggccttgtt gaaatctcga ttccaaaaca
    34441 tgaattaggc gctacattag ccgtgtaaac aaattttgaa cataataatt aaagagcgtg
    34501 ggacaaaagt aaagaacact tttgtctcac gctctaaaca cgtataaacg gcgaaagcag
    34561 aagcaactcc ttcggaaata agctgaaatt cacgaaaatt gcttttcaaa tttttgtgaa
    34621 ttccagctta tttcttggag ttaaacgctt ctgtctcagc ctctttttct ttctagcttt
    34681 ttgatttttc tcttttcttt tgtacttttc tcttttcatt ttcagttcta cgcgtgtctt
    34741 tatttttaac tttttcctct ttatggatga ttgatcgtac tttttgtgca atttgctcgt
    34801 ttttgtccca gtcttttaca tctaataatc gacataatcg taacatctct tctttttctg
    34861 tttttttctt tcacgtcttt gccattctgt ttccatgtgt cacactctcc tttctttaat
    34921 catatgtacg caaaataatt tttatatttt tttgattaga catttctatg attttaatat
    34981 agtaaaaaga gccgtcacgg aaaatccgtc acggctctaa acaaaatatg agaggctgtt
    35041 tttgtttggc tattcttatt acttgatgca gggcagcttg tgtcaagact tctttacatc
    35101 aaacttttta attttacatt gggatattta tccgcaaacc agcgttctgc aaattggttc
    35161 tcgaataaga acaacggttg gtcgaaacgg tcacgagcca agatattgcg acttgaactc
    35221 atcttctcat ccaaatcttc tggatcgatc cagcgagcga ttttgtgtcc cattggagac
    35281 atgattactt cggcgttata ctcatttaac atacgatatt ggaatacttc gaattgtagc
    35341 tgaccaacag ctccaatgat atattcttcc gttagatatg ttttataaag ctgaatcgct
    35401 ccttcttgaa ctaattgata gattcctttg tggaaggatt tttgtttcat gacatttttt
    35461 gcagtcactt tcataaacag ctctggagta aatgaaggga gttcttcata agcaactttt
    35521 agttttcctt cgaacaatgt atccccaatc tgataattgc ctgtatcata tacaccgatg
    35581 atatcgcctg ctactgcttt ttcgacgttt tctctcgaat ctgccataaa ttgtgtgaca
    35641 ttgcttaatt tcagtttttt atttgtacgt tccaaccaga catccattcc tcgttcgaat
    35701 gttccagaac agattcggac aaaagcgatt cggtcacgat gggctggatt catatttgct
    35761 tggatcttga atacgaaacc tgaaaattct tcctcataag gacttaccat ctgatcatct
    35821 tgtgttttgt gtccataagg agctggtgcc aattcaacga aagtctctaa aaaagtctgt
    35881 acaccgaagt tagtcaatgc agaaccaaaa aatactggtg tctgatcgcc acgagcaatt
    35941 ttttcttgat taaatgcatc tccagcctct ttgattagtt ctacttcttc taagacttgt
    36001 tcatataagg aattttcagt tagaggatga ttttcgggaa gcagtccatc ttcatccaat
    36061 tctactaatc gttcatcctg ataattttcc ggacgataaa actctacacg attatggtag
    36121 atatcataaa gaccttctag ccctttaccc ataccgattg gccaattcat cggataagat
    36181 tcgatatcta atagctcttc taattcttct aacaattcca atggttcacg accatcacga
    36241 tctagtttgt tgataaaagt aaatatcggg atgccacgtt tttttacaac ttggaataat
    36301 ttctttgtct gtgcttcgat cccttttgca ctatcgatta ccatgaccgc actatccacg
    36361 gccatcaaag tacgataagt atcttcagaa aaatcttcat gtcctggtgt atccaagata
    36421 tttactcgtt tcccttgata atcaaactgc ataacagaac tagtaacaga aatcccacgt
    36481 tgtttctcga tttccatcca gtctgatttg gcaaaattac ctgttttttt gccttttacg
    36541 gtcccggctt gacggatagc cccaccaaac aatagtaatt gttctgtaat cgttgttttc
    36601 ccagcatccg gatgggaaat gatcgcaaaa gttctgcgat tgtccacttg ttgctttaat
    36661 tttggattat tcataaccta actccatttc ttccattcaa ttatttatat tttctctagg
    36721 cacagattgt ccgatctctt aaagaaagac aatttcatat agggctgtga caaaagccgt
    36781 gtagcagtct ctttttccga gtaaacggcg ttctagagac agcctatcag caataagccc
    36841 acccaagcca aaaatacgaa aagctgtttt tgcttggctg ctcttattac ttaatgcagg
    36901 atagcttttt cacaaccttt gtcaactact acaactgaat cagtatagca tggattcagc
    36961 gaatgaaaaa gccaccaaac cataagagtc aaatattttt ttgtcagctg actaaaagaa
    37021 aagacgtgtt gcttgtttat tcaagcaaca cgtctttcat aaccaagttt ccaattatct
    37081 tcttggctcg tcatcttcga attcttttct gaagtatctt cgttcttctt caggttccgc
    37141 ttctgtttct tccggatcat gaaaaatgac acggatttta aggattcgag aaccttccat
    37201 ttcttcagaa accagcgtaa tattatcgac atcaaacgat aacttttcgc cttcatccgg
    37261 aatcatgcct aatgctgtaa tcaaataacc agccatcgta tctacatcac tcatatgcag
    37321 atcactgtca aaagcttcat tgaactcatc aatcagcata cgtccttgga tgatatattc
    37381 gtgttcatcg attttttcat aaagattctc aacttcatct gattcatcat cgatttctcc
    37441 gacgatttct tctaaaaggt cttctagtgt tgctaaccca acgacgccgc catattcatc
    37501 taataaaata gccatctgat tctgcgtctt tttcatttca tataacagat catcaataaa
    37561 gaccgtttca ggaacgaata aaggctcctg catgattttc ttgatatcta aattatcaaa
    37621 gccgaattta tgggcagctt ttagtaaatt ctttgtatga aggattccga ctactttatc
    37681 tttgtcttcg ttataaacag ggattcttga atagttttcg gataaaactt cattcacatt
    37741 ttcttccaca gtgtcgttga tatcaatcat aaatgcatcc gttcgtggga ccatgacttc
    37801 tcgggcaact ttcgtatcaa gagaaaaaac cccttggagc atttctaatt cttcattatt
    37861 caatacgcct tcgttttcaa gcatataacg catttcatct ctggtcattt ttgaatcttc
    37921 atcatcaaaa gtcatcggcg tgatacgcgc caataaactt gtggaagcag aaagcagcca
    37981 gacgaagggc ttagctacta caccaatcaa gcgtatcagc cctgaagtga actttgcgac
    38041 ttcttctgat ttatttaaag caatacgttt agggtacaat tctccaaaaa caatcgatac
    38101 ataggtcaaa atagccagca caatgatatt tgcaatattt tttgccgctg tgccgccacc
    38161 taataccgga gctaaccgtt ccgataaagt attagctagt gaagcaccag acaaaatgtt
    38221 gaccaaagtg attcctactt ggattgtcga taggaaatta tttggattct gcaatatttt
    38281 taaaagcttt tgagatttca catctccttc atcagctttt tgctcgattc gattcttatt
    38341 gatcgaaaca acagcaattt cggaagctgc taaaaaagca ttgatcaatg tcaaaacgat
    38401 caataataaa atctgcgcta acagcgactg actctcaggg tcagcattca taatcgcatt
    38461 ctctccttat aaaattaatt tttagtttac gatagaaaaa aacgtggcac tatagccacg
    38521 ttaaaatggt atcacaaagc aattttcttt gcaataaaaa caagtgtact aaaactacct
    38581 acgaggtcgt agcaattttc ttcttgtttt cattttgctt gtcttctttg acgattttga
    38641 ttatatagga aatgatcgtc ttgcagattg cgtaaaaagg aacacctaag aagataccaa
    38701 gcaatccggc aatatttcca gctactagaa gaacgataat gatcgttaat ggatggattt
    38761 tcagtgattt cccaattaca ttaggataaa tgatattccc atccaactgt tggacgatta
    38821 gcacaaccac acaacaaagt aatgctttta ccggatcatt gaagactgtt actagaaatg
    38881 caggtgctag tcctagataa ggtcctaaat atggaattag gtttgtcaat cccgcaatga
    38941 ccccaaataa gaaagcataa tccacaccaa ttaccatata tcctaaaaat gtgaaggtac
    39001 caacgaataa acattcgatt gcttgtccgc taatgtaatt agccaatgtt ttattcagtt
    39061 gccccaataa ctccacaatt tggtttcttc ttttttctgg gaaaaactgt tggatgtttg
    39121 gcactagttt ttctccgtct ttcaacatat agaacaagat gaatggtgct gtaatgatta
    39181 ctacggttgc cgaagcgatc gttccaacga tcgaacctaa actattggat actccactaa
    39241 ggaattgctg aatgattgtc ccgtaagaaa tgtctaattg ctcaaaatat ttattgatat
    39301 ctacttcctt gaacaaaggc aattcggcta cctcttttaa ccagctttct acctgtttga
    39361 tgaaatcagg catattagat gccaatgagg aaatttgctg tactagactt gggatgacac
    39421 taagtaaaat ccaaataatt gctgcaacta aaaggagcag cacaattgcg acagcccata
    39481 ttcttttgat ttttgctttc atcaacagct tgacaatagg attcaataaa tagtaaagaa
    39541 agcctgcaat cagtacaggt gcaaataacg tggaaaaaaa tgtcccgatc ggttcgaaaa
    39601 taaagtttat ttctgatgaa atcatgatca atgcagctat gatcaataat tcaagcgacc
    39661 aaaacattag tttagactgt ctaagttttt caaacaatag atttcctcct ttctttctta
    39721 ctagtatata ttatattcgt taaaaattct tttattcttt ttttattttt gaaaacttgc
    39781 catacttttt atttgtttta ttttccagta tactatacga aaggaaggtg gctagaccat
    39841 gaaagttgta caaacaaaag atacaatgag caatatttac ttagatgctg tacggatcag
    39901 gcatcaagtt tttgtagtcg aacagggtgt gcctttgtct cgagaaatag acaaagatga
    39961 agcccattgt atccacttcg tcctgtactc agataaaaaa gaaccgcaag gaactgtacg
    40021 tcttttgcct ttagaaaacg gaaaaatgaa attacagcgg atggcgattc tttctgaata
    40081 cagacaccaa gggttaggaa aaatattgat tgaagaagca gaaaactttg ctaagaatca
    40141 aggctataat acgattttat taggggcaca gtccacagct gaaacttttt acgaaaaact
    40201 aggatataca gcttacggcg atccttttga agatgctggc atgcctcata tttatatgaa
    40261 aaagacttta taaaaattta tttcgaaaaa agcaaaaaac agcaagcttt tgagaaaaat
    40321 ctctcactgg cttactgttc ttttttgtgt ttacttacgc gacaattgct tgtaacggtt
    40381 ataccaaata tcaatatagc cttccgagaa cgggccatct ccattattga tccagtcaac
    40441 cagaatcttc acattttctt ttaggatgaa gtcaatatcg ttcgggtaat tcatcatttt
    40501 gctgtgcatc tcatattcat cgacatccaa tagtcgtttc tcgccgtcag gaaatacctt
    40561 gatatccaag tcatagtcga tatatttcaa ggcctcatcg tccagcaaaa aaggtgaagc
    40621 tagattacaa taataggaaa cccccttttc tctgatcatt gctattacat tgaaccagta
    40681 ttttttatga aaataaacaa tcgctggttc gcgcgttacc catcggcgtc catcagattc
    40741 agtgactaat gtgtgatcat tcacgccgat catagagtgt tcgcttgttt ttaataccat
    40801 ggtatctcgc cacgttcgat gcaatttgcc gtcgtgcttg taactttgga tcgtcacaaa
    40861 ctctccttct tttggaactc ccatagcaac ccttccttaa gcagcatttt gtgctttgtg
    40921 tttgggtgtt cattacaccg acatcataat tattatatca ataaaaatga gctttgccta
    40981 gaaaggattc ctattgtttc gatttttctc taactgatct accagtttca tttggacctt
    41041 aggaaaaacg accgaatcaa atgcatcagg cttcaaccac ttcgaagttt tgttctcggt
    41101 aaattcttgt tctgctccct cggttgcccg tccgtaaaaa aggagaacat gccatttcag
    41161 atgactaaaa atatgagtaa cttctcctaa atgtcgtgtc tgccaaacaa agagttcctg
    41221 cttttcaaat gggagatttt gctcttcttc tgctactagg tcatcaaaca gatcgatttc
    41281 tggctttgtt tcccattctt ttttcaatcg ctcatactcc tcttgtgtca cttccatcat
    41341 tggaaatgtc cacatattcg ccagcaattt ttggctatct ctttcttcaa aataatacgc
    41401 tccagaatga ttttgaagag ctgcactgat ataataaacg tcttttggct ttgctttttt
    41461 tgtttttaca ggaaaactcg tttggattcc tcgtttgtta gctaaacaaa aggcttggat
    41521 cggacaagtc tcacactttg gagaagttgg tgtacagata gctgaaccta gatccatcat
    41581 tgcttggttg aattcacctg gatgcttttc atcaatgatt ttccgcatcg cttcatcaaa
    41641 gattttcctg ctagaagctt ttgcgatgtc tgcttcaatg caaaacaatc tgctgactac
    41701 tcgcatcacg ttgccgtcaa ctgcaggttc tggaagtcca aatgcgatac ttgcaatcgc
    41761 gcctgtcgta tatggtccta ttcccttcaa tgagctaatt tcttcaggcg tttgcggcat
    41821 ttccccatca aattctgaca tgatctgttt agcagcagct tggatgttcc gggctcttga
    41881 gtaataacca aggccctccc atgcttttaa aagcttttct tctggagcat ttgctagctc
    41941 ttcaattgta ggaaaccatt ccatgaaacg ataaaaataa tcgatcactg tgtctactct
    42001 ggtttgctga agcatgatct cagagatcca gatacgataa ggatcacgat tatagcgcca
    42061 aggcaggttt cttttttctt gttcatacca ttgaataaac tgatcttgaa attcttttgt
    42121 ttctttatct gtccattttt cccattcttt attcactttc ctcttccttt tccgtaatga
    42181 atcgctggat catatccaaa tcaaatcctt tttgatataa gctttgcttt acttttagat
    42241 ttcttttttt aggttcaaac cttaaatttc ttcgccatag tttatctcct tgtttgacta
    42301 aataatcata ttctatttct tgatctgctt cttgcggaag atcagctatc gcttgttgga
    42361 tcacttcttg agaaaagcct ttcgtcatca atgtttgttg gatttttctc aataattctt
    42421 tttggctttt tccgtgattt tttccaaacg ctttttcggc tgtctgatgt gcattttgaa
    42481 cttggtcttc aaatgtatat tggctgagcg cttgttcaat gatttcaggt ttcaaacctt
    42541 tttgctgcat ctgctgcgct aatttctttg gtcctttgtc acttaagcgc atattcgtgc
    42601 gcacataact ttccccatag accaaatcat cgacaaggtc caattctttt aagcgggcta
    42661 cgattttatt tcgatcttct tgaaggattt ccttttcttt taagtatgtt cggatttctt
    42721 tttccgatcg caactgataa cttaagtaat tcattgctag gtgaaaaccg aaatcagctc
    42781 cgctactctt cttgatctct tggatagttt cttcgtctaa ttcccgtcct tttaataatc
    42841 gatagcgtac taatacatct tcagatactt gtaaagcttc tccttcagat aaatctacgc
    42901 gatagaaagg gcctttgcct ttactgattc tcgtaacggt taacataaac atcctcctcg
    42961 aacatatatt ctctatcata ccacagaaaa tccccgtaat caggtattat atgttagaat
    43021 aaaccacaga aaaaagaaaa agggagatac ttatggcaga aacaattgtc aaaactggac
    43081 aaacggttcc tttgaaaatc aagcgtttag gcataaatgg tgaaggaatc ggttatttca
    43141 aacggttgat catatttgtt ccatatgcac taccaaaaga agaagtacta gtgaaaatca
    43201 cgaaagcaac tcctcgctat gcagaaggag aattaataaa agtcaaaaaa acaagcaaag
    43261 atcgcgtagt ggcaccatgt cctgtttact atgaatgcgg tggctgtcaa ttacaacact
    43321 tagcttatca agctcaattg gattttaaaa aggatctgct tttgcaagca ttggaaaaat
    43381 tcaaaccagc aggctttcga tcttatcaac ttttgccgac gatcgggatg gatgagcctt
    43441 ggcattatag aaacaaagca caatttcagt tacgaaaaaa taagcagact caaaaaattg
    43501 aagctggttt atacgaagca aactctcatc agttagtccc tataacagac tgcttggttc
    43561 aagaacctcg aacacaaaaa gtaatgaata ctgttgttca actattgaat aaatttgatg
    43621 cacccatcta tgatgaacgg acaaacagtg gaatcttccg cacaatcatg gttagaatcg
    43681 gtgttaaaac tggggagtta caagttgttt ttattactcg cagtaaaaaa ttcccgcaaa
    43741 aaaacgcaat gatccgtgag atcaatgaac gactgcccga agttgtttca atcatgcaaa
    43801 atgtccaggc acaaaaaacg tctctcgtga tgggggatga aacgatccac ttatggggaa
    43861 aagaagccat tgaagaacaa atcaatgaag tgactttcga tctttctcct cgtgccttct
    43921 ttcaactcaa tcctcagcag accgaattgc tttatggcga aggaatcaaa gcattagatg
    43981 tccagcctaa tgaaacgatc gtggatgctt attgcggagt tgggacgatc ggcttaagtg
    44041 tagcaaaaca agccaaagaa gtacggggaa tggataccgt tcctcaagca atcgctgatg
    44101 caaaaaaaaa tgcggaaaga ttaggatatc acaacactcg atatgaagtc gggacagcag
    44161 agcaagttct gcctaaatgg ttgaatgaag gttttcagcc tgatggtatc atcgtagatc
    44221 caccacgtac gggtttggat aacgcattga tccaatcatt attgaaatac cctccaaaaa
    44281 aacttgttta tatctcctgt aatgtttcta cacttgccag agatctggtt aatttaagta
    44341 aaaagttcaa gattgaatat ttacagtccg tcgacatgtt tcctcagact gcacgttgtg
    44401 aagttgtcgt gaagatgacg agactcgctg actaaatgaa agaggttgtg aaaaagtctg
    44461 ccctgtatca agtaataaga acagccaaac aaaaatagct cttcatgttt tatgaatact
    44521 gtacttgtga ccgaagagtt aacaggagga cactatttat ttaaaaaaag ggttgtgaca
    44581 tagtttttgt cacagccctt tttgattatt attggatatt cacgtctatc acttgataaa
    44641 atgcatttgt cgtatcgctg atattccata ctgctaaaat cacattatat ccttttcgat
    44701 cctgcggaag tgtgatcgtt tggttgactg tttttggtgg aacagttgct ttgtcgtcta
    44761 tttttgtgat caattcaaaa tcactaaatt tcaaaggttg attaggattc caagttggtt
    44821 tggtaatgta gtaatcccaa gtacttgtcc gatgttgtgc tgttaaattc caagtaatat
    44881 ttaatgttcc tggattaacc atgtttttat gccatctgtt agatgtctgt tcatctaaag
    44941 gctcaaagcc gctgactcct gcactagcaa tttttccatc aatgaatgta tttttggtag
    45001 cttcgatact ttgcggttcc cattgtgctc ttccgacatt ttgatttaag tttcctgctg
    45061 ctgatgttcc taggtatgca cggcttcctg gagaagtaat ataaccatgc gcaaaacctg
    45121 gaatgctaaa accgaacaac cctaaactaa ctaacccaat aaacaataac gtactaatct
    45181 ttttcatgta gaaacctcct tcatttgata accacattgt aaacgcttta ttgtattaac
    45241 acaaacatta tgcttttata attttatgtt gaagtaaaaa aggtgaaaaa agtaagatgc
    45301 agattataac agaagtagac gagaaaagta caaatagaaa acatgagaat caaatcatat
    45361 gatctttttc aaaaacatca ttcatataaa caaatgttca actttttaat aacaacaatt
    45421 atcaattttt ttcttacttc acaaactaaa tacaggtctt gctgtgagcc ttcatggtaa
    45481 actaatatta tagaaaggag ttagtaaaat gaagatgata aaagatattc gtttttgggt
    45541 ttgtgttata ggaattgtca tattaggctt tttatcaggg ttgcttagcg gaaatcccgg
    45601 aaaatattac tattctttgc aacttccacc atttgcacca cctagctgga tatttgggcc
    45661 gatgtggacc ttactgtata tattgatggg tatttctctt tatttgttac ttaatcacaa
    45721 caacaaaaaa cagcgaaaca atcttgtcgg tctttttgtg attcagttca tttttaattt
    45781 tatatggtct gctctatttt ttaatttaag aaatattttc attgcagcaa tcgatattac
    45841 acttttagtt attttcttgt cagtgcttat gtatcagttg tggctgcatc atcgcctgac
    45901 aatgtggctc atgattcctt attatttatg ggtactattc gcaactctat tgaattattc
    45961 aatttatttt ttaaactaag cgctaaaaag agacgtcctg taatttaaca catagaaaaa
    46021 gtgccaccta agttagattt tttgtctaat ttaggtggca ctctttatta accaaactat
    46081 ttttttctag tacccacttg ttgttccata ctcattattc tggtcataac cataaccatt
    46141 ttgttcctgt ccagcgtaag gatcataact ttgagtatct tgacttggtg tatagtactg
    46201 acttgtatct tctgtttgct gataataggt attcggatct tggtactgat catatgtgtt
    46261 ttctgaatag gaagaataat cggtgtaccc ttcactattc gatccatcac cgtttgtcga
    46321 atcgccatct tccgaatcat caaagaacat cattgaagca ttctcatttt tcaaattagg
    46381 taatgtatcg cttgttttta tatttaattg tttcttcagt tcattttgga tcgaaagaag
    46441 atcatttttt cctaaaattt gatagtagat atcattgatc atttgtcctt cgccttgcaa
    46501 ctggtcttgt ttgattgttt caaacgccgg ggtgtagttc gtcgctacat caagcatgtc
    46561 atcccaatct aagtccgtcg tcacattgtt ctcaactgct tttaaaattt ttctataatt
    46621 gcttacgcca tctaaactta aaactttatt gacgattttc gtcactactt cccgctgacg
    46681 acgttgtcga ccgatatcgc cttctggatc ttcgtgtcgc attcgagaat aggctaaacc
    46741 tttttctcca ttcaatgttt gttcacctac tggtacatgg acaccatcca aagtaaagtc
    46801 aattttatta ttcactttga tgccaccgac cgcattgatc aaatcttcca tgccatccaa
    46861 attgatcgtt acataatgat caataggaat atcgagcagt tgctcaattg aatccatggc
    46921 catttcgaca ccaccaaatg cgtaggcatg atttaattta tctaccgttc cataacctac
    46981 gatattcgta taaatatctc ggtccaaact gatgatcgtt gatttttttt gtttcggatt
    47041 aactgttact accatcatcg tatcagaacg tccttgttcc gttcttccta atccaccggt
    47101 atctaaacct aatagcaaaa ccgaaaaagg ttctttgtca tcaatgctta ctctctcaga
    47161 acgtttggag gatattcgat tagagttttt agaaattttg tttactgttt ggctcgcttc
    47221 tcccataaat ctgattccgt atgctgataa tccggcaatc agcaaaacga atacccctaa
    47281 caaggttaat agaacttttt gccatcgctt cataatacac ctctgtttca cattttctaa
    47341 aatattgtct catataactg ttcaatcagc aaattcactt tttaatcata agaaaacttt
    47401 aaaaaacatt tttgtgtaac gattattatt ttttaaaatt tctcaaaatt cttaactatc
    47461 ataccatacc gtgaaaaaga tcgaaagacc gaatacacgc agttcgcaat cgttttatct
    47521 ttttttatgt attttttcca gaaaaagcga acaacaaggt attttccaaa taatcgtgca
    47581 gtaaaatgat cgcacatacc tttcccatct ttttaacagt ctttgcctac cgttcagatt
    47641 atcattcgat ttcacgacaa tttcttccta tgactggtcc ttttgttaaa cccatcttct
    47701 attcgtttta tttccagctc actgcatggc tcttaaaatg actcgaacta gtgattgcat
    47761 taccatcatc ttttaatgga tagatatgct cctcttcaaa gtaaaacgat tttcttttaa
    47821 agtaatagaa ttgacatggt aatgagtatg tatactagtg gtttcacaaa tggtctttta
    47881 ttcaacatcc aaaaactgaa atttctttaa aatccattac taacgaagaa aatgttcctt
    47941 gcttgataat tagttaggac atgtcaagtg aacaaatgca tttacttagc caaaaaatct
    48001 aaaaacttca taaatagaca aaaagacacg actctctaag aagtcgtgtc tttattcaac
    48061 aaattattat aatttagtca cttcagcagc ttgaggtccg cgtgcacctt caacaatcgt
    48121 gaattctact tcttgacctt cttctaggct cttgaagccg tctccttgaa ttgctgagaa
    48181 atgaacaaat acgtcatctc cgccgtctac tgtaataaac ccaaatcctt tttcattgtt
    48241 gaaccattta accgttccgt gttccataaa tgtatatcct ccaaaaaagt tcttgcaagt
    48301 aaccttgcaa gaacattata cccaatttag aaaacgctag caagctttcg ctcttttatt
    48361 ttccaaatta tttttaagac tgatatgacg gaggaaatgg ttattttgct gcttcgaaac
    48421 gtttgtttac ttcatcccaa ttaataacat tccagaatgc tgcaatataa tctggtcgga
    48481 catttttata tttcaaataa taagcatgtt cccaaacgtc caaacctaga attggtgtct
    48541 ttccttccat caatggagaa tcttgatttg cagtagaggt aatagctaat tttccatttt
    48601 ccattacaag ccaagcccat ccagaaccaa atcgtccagc ggctgctttt ttgaattctt
    48661 ctttaaaaga agaaaaatca ccaaaagctt cattaatcgc ttcttttatt tctcctgtag
    48721 gttcgccacc agcatttggt gccatgattt cccagaaaaa tgaatggtta gcatgtccgc
    48781 caccattgtt acgtacagct gtcttgatat ctgttggaat agcgtccata tcagataata
    48841 attcttctat tgttttttcg cctaattctg ggtatttctc aattgctgaa tttaaattcg
    48901 tcacataagt attgtgatgc ttatcatgat gcagatgcat cgtttcttcg tcaatataag
    48961 gttccaatgc gtcatatgca taaggtagat ctggtaatgt gtaagtcata aaaaaattcc
    49021 tcctcaatgt tttcttaata atgtctacat tacaaagagt aacaaagaga aggaaattct
    49081 tcaattgtta tgcctagtcg tctttttcac tttgacctac ttcttcttca aaaacctcag
    49141 tttctttaaa aataaacaac ttgctgaaaa tatagttcgc aaggataaca ataatttgag
    49201 tgatgatttt cgccacatac tcattgctgt tcataacatt gatcaacaat accattgccc
    49261 ccatgtccaa tccgtaagaa aggatacggt agaagaagaa cttaccgcat tcaataagtg
    49321 catgtgtaaa attcggtgat tttgaatgga atacccaaag tttgttcgtt acaaaagcaa
    49381 acaatacaga acaaaaccac gaaatagaat tggataaggc catacttaaa tcaaaatatt
    49441 ggcgtgcgag agcaaaacta acaaaattta caatcgtagc caaaccacca aaaaagagat
    49501 agactagtat ctcccaatat ccttttgctt ctaaatatcc ttttaactgt cgatactgct
    49561 tcatgagcca acctacctta ttaaaaattc ttatttatca tactctatat ttcaataact
    49621 ttcatcttta tttaaaaaaa ttaatttttt ttgatccttt gcctttgtct ctgcttggat
    49681 cgtcacatgt gtaataccat attttaattt taataaagaa attatacgag gatatatctt
    49741 ttcaatctcg cttagtagct gatcttcaaa atcaatatgt ccgtcgaaaa taattatctt
    49801 ttcgctatac tgccaaatat ggacatgatg aacatccttt accccatcga ttgcttcaat
    49861 ttcttgttcc attttttcat agtcgatagc aggtgcactt tgtatcaaaa tagcggcagc
    49921 ttttttgatc accattatcg cttctatcaa tatatatatc gaaatgatca gtgtaataac
    49981 tggatcgatc cagtagatat cgaaaaaacg gatcaaaatt gctccgataa tcacacttac
    50041 cgaagacagt gtatcactta acaaatgcaa ataactagat tttatattca aactttcatc
    50101 tgcttcttgt cgtaataata atactgagaa caaattcgcg agcaatccaa taactgctac
    50161 tgttagcatc aaatgactat ttatcttttc tggtgaagtg aaccgcttga atgcttcaac
    50221 ggttaaaaac aaggataata tgatcaatat gaatgagttg atgaaagcac tcaatatttc
    50281 taaacgctgg tagccatacg tacgtttttt atttgcttct tttcgggcca ttttttgtgc
    50341 cccgtaagaa aaaataattg ccaatgtatc actaaggtta tggaaagcat ctgaaacaag
    50401 agacaaactc cctgacataa tccctccaat aaattctgat aaagtgatca ccgcattcaa
    50461 aaccactact ccaaatagtt tttttgatga tctctgtggc tgtgtaggca ctattccttc
    50521 ctccatttta tttatattgg atagtcttta gacaagaata acattttttt gatatttcac
    50581 caatcattca gctgtttcct taaagcactt gtatttcact tgatacttcg ctagaattgc
    50641 aagtgatgac actactagat gaggagtact tatgacaaca aaacaattaa ttatcagttc
    50701 ctttatacga ttcaaagacc taaaggaggc gagaaatgtc ccattttgga aagccattgt
    50761 ctatctttta gttttgagta taattatggc tttgccaatc tcttttcaag tttttcaagt
    50821 cttaaacaac attcaattgg atgggcaaaa gattgctact cgcattcctg attttacaat
    50881 ccaaaacggg cagcttcaaa ccgaagaaaa agaaggattc atttaccaaa caaactccat
    50941 tatcttcaca tttgatccag agggaaaacg aacagaacag gacatctcta ctgatttgat
    51001 ggggaatttt gttagtgtcg ggatgttaaa aaacaaactt atcatagctc ttcctaatac
    51061 aggcacgact tctgctttgt tgaacaataa ccaattagaa cttccctaca ccaatgaatc
    51121 tttaaaaaac ttaacaggta agcagctccg ttctttttta agtgaagcat ctataccact
    51181 ttggataaaa gtcctgacat ttatattttc tatttatcct agttttctga atctgctgat
    51241 cactttgcta tttaccaaca ttgctgcttt tctctatgca cgctttcgac taacaaaagc
    51301 tacttttctt gattgtttaa aaacattgat ctattcaatt tcattgccta ctataattgc
    51361 aacaatcttg atggtctttc ttccttcttt tgacactagc gccttcattg cttttgcagg
    51421 aatctttatt tttgctcaag ctgtcaaagg atgggcaaaa atatcgatca attaagaaat
    51481 aaagacaact cgttatttct acttcaatag acccttttag aggttgtgac atttttttgt
    51541 cacagcccct tttttatttg aaaaatattt ctttctcaaa aaacaagcaa ggcgcttcaa
    51601 agttcttctt gtatgcgcta cacttagagt aaagaaaggc gggaaattaa ctatgtcaga
    51661 aagaatcatt tatgcagatc gtccatttcg tgatgaattt atcgaagaaa tccatactat
    51721 agcgcctggt tatatcttta aaacagaatt acaaccagag gatctacaaa atgtagagat
    51781 cagtttgggc tggaactcag actaccaaga aaaactatta gcctcttcta ccttgaaatg
    51841 ggtccaatct atctcagctg gtgttgatca gctacccctt gagacttttt caaagaaaca
    51901 tattttactt tcaaatggta gtggcatcca cagtgaatcg attacggagc atgtcttagg
    51961 aatcatcctt gggtattctc gtggtctttt tcaagcacaa aaggcacaga gtgacatgaa
    52021 atggttaggt tcatcggtcc attaccaatc tatccaaggg aaaaaaatac taatcattgg
    52081 aactggccat attggaaaaa tgattgcaaa aaaatgcaaa gcattagaca tgaactgcta
    52141 tggaatcaat acatccggtc atccagcaga aggaatggaa aagacttttt ctttaaatca
    52201 actaaaggaa gttcttcctg aagtagattt cgtagtcaat atcttacctc taacggagga
    52261 aacaactggg ctctttgatc agcatatgtt tgaacacttt gatccaaaat ctgtattcat
    52321 caatgttggc cgtggtgctt ctgttaagac acaagatctg gtgcaggctt taaataacca
    52381 gcaactttcc tttgcagcat tagacgtttt tgaggaagag cctcttccac aagatcatcc
    52441 tctttggaaa atggacaatg tactgattac ttcccacatt gcaggtttga cacctgattt
    52501 tcaaaagaaa ttaatggcta tttttttaac caatctgaaa agctactttg ccacccatga
    52561 gcttcaaacc aatcaagtaa aattgtctgc aggatattaa tagctgaaaa caatattttc
    52621 aaaagaaaaa agagaaggtg acaaaaattg tgtcaccctc tcttttttta ttgtttttta
    52681 aagtgatagg ttggctcctc caggttggga tcataatcaa caagtaaatc atatccttta
    52741 aagaaccatt catctgcttc ctcgataaaa aaaagtgtcc catccatttc cactttcttt
    52801 acaggatgtt ctggctgttc aactgacatt ccaatcgaaa aaccatcatg aacctctgtt
    52861 ttgccgtaga cttttccaaa aaaacgtatt ccgtaagttt ctggcagatc aatttctgag
    52921 tcaaaccact tttgtgcttt tggcgttacc gttaatttca tcccaacaac ctactttctc
    52981 ttttctttct attgattcat cttatcgtct agaatatcca catcgcctgt ctctccaata
    53041 accagtagat tatcattttc acgaacatat tcatctgctg caggagaagc gataaccgtt
    53101 tgatctactc ttcgtatggc gactactgtt aagtcaaaac gttgcctgaa atttaattca
    53161 gctaatgttt tgttgaaaaa tttaggattg gagactcgga tttctgctaa agaatattta
    53221 tctgacaact ctaggtaatc taaaatattc tttgacacta aattatgcgc aatccttatt
    53281 cccatgtctc gctcgggatg gacgacgcga tcagctccga tcttttcaag tactctggca
    53341 tgatactcat tttgggcttt tgctaaaata tttggtacgc ccatttcctt taccatcaga
    53401 gtaactaaga tactggcttg aatatcttct ccaatagcga caattacatg atcgaaattt
    53461 cttatgccta atgatcgaag tgtcatttca tcctgtgcat ttgccacaac agcatgagtg
    53521 gctatattca tatattcatt gacccgatct tcattactat caatagccag tacttcttga
    53581 ccagcttcga tcagtgttcg gcagatactt ccgccaaatc gtccaagacc tacaattgca
    53641 aaattttgtt tcaccgtttt cctcctttag tatcttcttt tctcagtata acaaagcctt
    53701 tacaaaaaca aaagcaagca tcattattgt aaaaaagcca agaaaaataa tagttatttt
    53761 ctcagctttt gattatcctc tttatcatcg tttttatttc accaaaccat gtttgaatgc
    53821 atagatagcg gcttgtgtac ggtcttccac ttctagctta gccaatatat tggaaacatg
    53881 tgtttttact gttttcagtg taataaacaa ttcgtccgct atttcctgat tactttttcc
    53941 ttcagaaatc agcattaaaa tttcattttc ccgattcgtt agttcttcat gtaatacagg
    54001 ctcatttcgc cgactcattt tttccatcat ttttgttgtc acttctggtt ctaatactcg
    54061 ctcgccacgt tgcgttgccc ttatagcatc agcaatttca tgtgcggtag aggtcttcaa
    54121 caaataccct gctgcacctg cttcaatggc aggataaact ttttcgtcat caataaaact
    54181 ggttacgata atgattttag cttctggcca atcttttaag attgcttttg ttgattcgat
    54241 accatccatt tcttccatga ctaagtccat taaaatgacg tccggtcgaa gtgtcatcgc
    54301 cttctcatag ccttgtcgtc cattttcagc ttctccaatt acttcaatgt cttcttgaat
    54361 agataaataa gaagagacgc ctaagcgcac catttcatgg tcatctacta ataaaacttt
    54421 tatcatcctt atctatgcct ccttcatcaa agggaccttg atttctacac ttgtaccttg
    54481 acctttaaaa ctaatgattt ttactgttcc accaatacca gctactcgtt ctttgatatt
    54541 gttcaatcca taacttcccg ttttcgtttc attcatatta aaccctgttc cgtcgtcaat
    54601 aatacgtaac aaaagattat tgtctatttt gtgcaaatat acctctaatt cattggcttt
    54661 agcatgtctt aatgtatttg ataacaattc ttgaacgatt cggaacaagt gatcctcaat
    54721 ggaactagat agttttacat cttctacatc ccatttcagc gaaatctgga ttttattttg
    54781 caattccttc aataactgct cgataccttg ctttaaactt ttctcttcta aattgactgg
    54841 acgtagatga agaagcagcg cacgcatctc agactgggaa gcgttgatga tctctgctac
    54901 cattttcaac tgcttgcgaa acatttctgg tgtctcgctt ttttctgctt gctccgtcaa
    54961 tgccgacatc atcatcattg ctgcaaacaa ttgttgcgaa actgaatcat gcagctcacg
    55021 ggctaatcga tgtctttcta attctaaaat ttcttctttg gtttgtccat ctacatattg
    55081 gggacggctc gttacaattt gtaattcact agatacttca atcatttttt cttttatctt
    55141 agtgatttcc ttatcaatat ctttgatgta taaatcgtcc gatgcacttg ggatcggaag
    55201 aagcaacaat ttggattcat aattgccaga agaaagtgca cgcagctttt cttcgatttt
    55261 cccgtatttt gtcttttgaa taagtgataa taatagaaag acaattaatc ctacacctaa
    55321 ggaaatggct aacaaatgga agataagcgg aacatataat aaacgtgcac gcagtaattc
    55381 tccccaccaa tgactttgat tgctggcata aaaataagtg aaaagtgaaa atagaataat
    55441 aaggaaagcc gcaatccccg aataaacagc tagcatcgct ctggatattt ttcccatcat
    55501 attcgtatca cctcgacatc tccaatcaag gtattagtaa taatcttcaa tctacgaggg
    55561 ttttcatcat aatcactgct gtagattttg atttgttcat ttttcaaaga aaattgctct
    55621 tcttcaaaca agacatttcc tactaatgtc gcatgctcta atcggatggc tacgcccaaa
    55681 ggtactaaga tccgtgttct gcctatccct tttcttacaa tcacaatatt atcatctttt
    55741 ggcaaaagcg tattgcccaa atctatgata gtatcaccag aaatgacggc gatgttgata
    55801 tcgtcccatt catagacgtc gtttccaata cgttggttac cgaataattg ctgtcttttc
    55861 cgctcattat tatgtgtttt taattgctct gtttggatca tcatgatctg tttctttcgc
    55921 caaaaagcat ttttggttaa atctatccct gaaatctcta cacctttcag accaataaat
    55981 aaaacggcaa atacagccat catccataaa gcaggactgt tgactaagct aaaaaaaatg
    56041 accaaacttc ctaatatcaa ttgaaaattt tgaaatttag tacgaggatg tttgcgcata
    56101 gctagatata tattgaaaat accgaatatc actaaaagca aaagtccagt attgtttacg
    56161 atttgccaaa cagcaaataa cagcagtagt gcttcaacta ccacaaaaaa acgccaagaa
    56221 ctattcatcg ctctttctcc ctcctgacac attctcattt catcatacct atttttttaa
    56281 ctgttggcta taggacttta gatgtaagat cgtttgttga aatctaaaat acacaaagag
    56341 aaaaagatct agagtgtatc atcagtaaga tgaactctct agatctttca gtcagactac
    56401 gcttgttcta ctttgacgat tttaactaac atatcgccac ctggagtagc aatagttact
    56461 tcatcatttt tacgtttgcc aatcaatgct tgcgcaatag gagaatcatt ggaaattttg
    56521 ccagaaaacg gatctgcttc agcgcttccg acaatcgtgt actcttcttc ttctccatct
    56581 ggtagttcga tgaatgtaac tgttttacca attgatacct catctgcatc tacaccgtcg
    56641 ttatcgatga tttgagcaaa acgaatcata ttttcaattg ttgtgattct tccttcaaca
    56701 aatgcttgtt catctttcgc tgattcatat tcagagtttt cggaaagatc tccgaaaccg
    56761 cgggcaattt tgattcgttc aatgatttct ccgcgtttga ctgtttttag ttcttctaat
    56821 tctttttcta atttttcttt accttctaga gtcattggat aaactttctc taccatgtgt
    56881 aacgtctcct tttttcgata aaataccgca aaaggtaatc ttttgaggaa tatagtccaa
    56941 aaaagattac cacgcttttc ttcaaatgta aatgtttttc taaaaatgat caatgtctag
    57001 agcctgtttt tctaagaatt atttacatat ttttccacta attccatatg ttcttcataa
    57061 gttttagcga aataaacatt tccagtgctc gtatctgcta cgaaataata atagtcattt
    57121 tctgctggtt ctaatactgc cttgattgcc tcttcgcttg gattgtcaaa aggtccaggt
    57181 ccatagccgg tattggtata caaattatat ggtgaatcta cagctgtatc ttcatacgtc
    57241 actaattctt tgtgttcacc taatgcgtat aaaacagaga tatctgactg aagtggcata
    57301 tttgctttga ttcggttaaa gaagacttgt gcaatgtttt tacgatcatt ttcttttacg
    57361 ccttcttttt caactagaga agctagagtt aaaacttctt gtacagtcaa atttttctgc
    57421 ttgatagctg aataatagtt ttgcatgacc gtattcgtct tgttcaccat ttcaatcact
    57481 agatccttaa gcgaagtact cgtgtaataa tcatatgtgg ctgggaataa gtaaccttcc
    57541 aaacggtatt ttgtgttctc agctttagat gcactttcca acagttctgg gaatttttgg
    57601 tgcaactcgt taaagaaagt ctcatcattc actaaatcta aaaattcttt cttgtcttta
    57661 ccagttacct tcgctacccg ttcagcaatc tgatcgatat cgtacccttc tggaatggca
    57721 attttcccat ctgcaacttt tgttggctca gaagtaccgc cttcttgcaa ctgtttgcca
    57781 atctcgtcaa gagtcatatt aggcgctaac tgataatagc cagcttgaaa gccagttaaa
    57841 tttttaaatt ttgtataata attgaaaaca atcccgcttt taataatatt gtccttctcc
    57901 aaaatctcac caatctgctt gttagaagag ccactcggga tctcgacttg gactaattgg
    57961 tcatctgatt tatctaaagg cttcaacccg ctatctacat aacgatacac ggtaaatcca
    58021 agaaatccgc caataatcaa taatgtaaga gcaatcacta aaatgatctt cctcacaatc
    58081 ttatcttctt ttttacgcag atttctccgc ttgtcgatta tatctgctgt agctgcctta
    58141 ctggcttctt ttttatcttt acttgggtgt tgtttgtttc ctgacaaaac aactcctcct
    58201 tactatcaag ttcgtaactt tattcattat acatgatatt tgaattggcg tgaatgattt
    58261 tatttaaata ttctatcaaa aatgtaagta gtaaatccat tactataaga ttaaaataaa
    58321 tgttatcaag atacaagcta ttttagcttt attctttctg taaaaaagaa aggagactaa
    58381 ataaactatt atgaaaaaag gaaaacatgc taaaaaaaga aaatattcat ttttaatgat
    58441 cgttgtcgtg ttgtttagcc ttagtgcgtt ctctacagga actattcttg gatggcaatt
    58501 ttacaataat gaacactctc agatagttgc ccatgctcaa tcagctcaaa aaaagttgat
    58561 actgactacc aatgaacaac agctcgttga aaaaatcgat caactattaa gaaaaaatga
    58621 ttatattggc tcaatttacg taaagaaaaa taatcgcatc atcatggaaa aaggctatgg
    58681 ctatgcgaat aaactcacgc aacaaccaaa tgatccttct ctttattatc agattggttc
    58741 ggttcaaaaa gcattgacta ctcttttgat tttaaaacaa gtcgagcgtg ggcacctttc
    58801 tttagatact aagctggctc aattctatcc acaaattgct ggcagccagc aaattactat
    58861 ccgtgacttg ctgtatatgc gctcaggctt gcaccgaact gcttctccaa ccataccgat
    58921 gactgatgaa gaagtcgttc aatttgcgat tcagcactta aaatttacag attatcagac
    58981 ctctcattat gattcattga actttaccat attgactgga attttgatca aattaacaaa
    59041 tcaaccgtat gaaaaattat tgaccgatga tatcatcgaa cctttaggat tacagcatac
    59101 aaaattttat gaccaagtca aaaattcatc caatcatgca ctatcctatc aaatgtctaa
    59161 agaggatgat tactttaaac cactgacaga atctgaaaca gagatccata acgaattagg
    59221 gactggtaat atcagtatgt ccgtctatga tttaaatacg ctgtttacac aagttttatc
    59281 tggaaaacta gtttccaaag acttactctt ctcattatgg cgtgaaaatg cgaatggaca
    59341 tccatatagt ggtggggtat atagcggaga taattatatc ctgtcgcaag gaaatatcga
    59401 ctcttttgat acatctgcta tatttagcaa agattcacag gatgctatca tcatggaaag
    59461 caatataaaa gctgacaaaa ctatcaaact gcctactgct gatctaagaa accaaatcta
    59521 tgacctgatg gaaggaacta ctaagttggc aaatagctga tatgatgaaa agatagcaaa
    59581 tactacttct ttgaatagca tttgcttaaa aaaagtactc gactaaacgt aggaaagagt
    59641 gtgggacaaa tctaaaaatc aatttttgct acagacttta attccaaata aacagaacag
    59701 aaagaggtgt tcttctttct gttctgtc
//
LOCUS       DACDPS010000009        54647 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.009, whole genome shotgun sequence.
ACCESSION   DACDPS010000009 DACDPS010000000
VERSION     DACDPS010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.009"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            46..119
                     /locus_tag="IWJ07_000622"
     tRNA            46..119
                     /locus_tag="IWJ07_000622"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:80..82,aa:Arg,seq:tct)
     gene            278..637
                     /locus_tag="IWJ07_000623"
     CDS             278..637
                     /locus_tag="IWJ07_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAP9332224.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            760..2391
                     /locus_tag="IWJ07_000624"
     CDS             760..2391
                     /locus_tag="IWJ07_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAP9332225.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(2453..3094)
                     /locus_tag="IWJ07_000625"
     CDS             complement(2453..3094)
                     /locus_tag="IWJ07_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP9332226.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            3376..3684
                     /gene="rplU"
                     /locus_tag="IWJ07_000626"
     CDS             3376..3684
                     /gene="rplU"
                     /locus_tag="IWJ07_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAP9332227.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            3711..4055
                     /locus_tag="IWJ07_000627"
     CDS             3711..4055
                     /locus_tag="IWJ07_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAP9332228.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            4068..4361
                     /gene="rpmA"
                     /locus_tag="IWJ07_000628"
     CDS             4068..4361
                     /gene="rpmA"
                     /locus_tag="IWJ07_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAP9332229.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            4768..7602
                     /locus_tag="IWJ07_000629"
     CDS             4768..7602
                     /locus_tag="IWJ07_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9332230.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            7793..8284
                     /locus_tag="IWJ07_000630"
     CDS             7793..8284
                     /locus_tag="IWJ07_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAP9332231.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            8387..9376
                     /gene="manX"
                     /locus_tag="IWJ07_000631"
     CDS             8387..9376
                     /gene="manX"
                     /locus_tag="IWJ07_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAP9332232.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            9409..10212
                     /locus_tag="IWJ07_000632"
     CDS             9409..10212
                     /locus_tag="IWJ07_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAP9332233.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            10233..11147
                     /locus_tag="IWJ07_000633"
     CDS             10233..11147
                     /locus_tag="IWJ07_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAP9332234.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFSPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            11230..11604
                     /locus_tag="IWJ07_000634"
     CDS             11230..11604
                     /locus_tag="IWJ07_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAP9332235.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            11743..11988
                     /locus_tag="IWJ07_000635"
     CDS             11743..11988
                     /locus_tag="IWJ07_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9332236.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            12160..13224
                     /locus_tag="IWJ07_000636"
     CDS             12160..13224
                     /locus_tag="IWJ07_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP9332237.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            13470..13895
                     /locus_tag="IWJ07_000637"
     CDS             13470..13895
                     /locus_tag="IWJ07_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9332238.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            13888..14337
                     /gene="nusB"
                     /locus_tag="IWJ07_000638"
     CDS             13888..14337
                     /gene="nusB"
                     /locus_tag="IWJ07_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAP9332239.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            14434..15288
                     /locus_tag="IWJ07_000639"
     CDS             14434..15288
                     /locus_tag="IWJ07_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAP9332240.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            15289..16626
                     /locus_tag="IWJ07_000640"
     CDS             15289..16626
                     /locus_tag="IWJ07_000640"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAP9332241.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            16631..16855
                     /locus_tag="IWJ07_000641"
     CDS             16631..16855
                     /locus_tag="IWJ07_000641"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAP9332242.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            16857..17747
                     /locus_tag="IWJ07_000642"
     CDS             16857..17747
                     /locus_tag="IWJ07_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP9332243.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            17756..18574
                     /locus_tag="IWJ07_000643"
     CDS             17756..18574
                     /locus_tag="IWJ07_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAP9332244.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            18681..19130
                     /locus_tag="IWJ07_000644"
     CDS             18681..19130
                     /locus_tag="IWJ07_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP9332245.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            19148..20830
                     /gene="recN"
                     /locus_tag="IWJ07_000645"
     CDS             19148..20830
                     /gene="recN"
                     /locus_tag="IWJ07_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAP9332246.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(20885..21001)
                     /locus_tag="IWJ07_000646"
     CDS             complement(20885..21001)
                     /locus_tag="IWJ07_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAP9332247.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(21074..22030)
                     /locus_tag="IWJ07_000647"
     CDS             complement(21074..22030)
                     /locus_tag="IWJ07_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP9332248.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            22201..22569
                     /locus_tag="IWJ07_000648"
     CDS             22201..22569
                     /locus_tag="IWJ07_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAP9332249.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            22739..23170
                     /gene="mraZ"
                     /locus_tag="IWJ07_000649"
     CDS             22739..23170
                     /gene="mraZ"
                     /locus_tag="IWJ07_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAP9332250.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            23191..24150
                     /gene="rsmH"
                     /locus_tag="IWJ07_000650"
     CDS             23191..24150
                     /gene="rsmH"
                     /locus_tag="IWJ07_000650"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAP9332251.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            24155..24556
                     /gene="ftsL"
                     /locus_tag="IWJ07_000651"
     CDS             24155..24556
                     /gene="ftsL"
                     /locus_tag="IWJ07_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAP9332252.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            24557..26749
                     /locus_tag="IWJ07_000652"
     CDS             24557..26749
                     /locus_tag="IWJ07_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP9332253.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            26775..27737
                     /locus_tag="IWJ07_000653"
     CDS             26775..27737
                     /locus_tag="IWJ07_000653"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAP9332254.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            27751..29112
                     /locus_tag="IWJ07_000654"
     CDS             27751..29112
                     /locus_tag="IWJ07_000654"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAP9332255.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            29138..30226
                     /gene="murG"
                     /locus_tag="IWJ07_000655"
     CDS             29138..30226
                     /gene="murG"
                     /locus_tag="IWJ07_000655"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAP9332256.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            30241..31461
                     /locus_tag="IWJ07_000656"
     CDS             30241..31461
                     /locus_tag="IWJ07_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAP9332257.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            31617..32942
                     /gene="ftsA"
                     /locus_tag="IWJ07_000657"
     CDS             31617..32942
                     /gene="ftsA"
                     /locus_tag="IWJ07_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAP9332258.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            32965..34206
                     /gene="ftsZ"
                     /locus_tag="IWJ07_000658"
     CDS             32965..34206
                     /gene="ftsZ"
                     /locus_tag="IWJ07_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAP9332259.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            34223..34900
                     /locus_tag="IWJ07_000659"
     CDS             34223..34900
                     /locus_tag="IWJ07_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAP9332260.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            34914..35513
                     /locus_tag="IWJ07_000660"
     CDS             34914..35513
                     /locus_tag="IWJ07_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAP9332261.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            35528..35815
                     /locus_tag="IWJ07_000661"
     CDS             35528..35815
                     /locus_tag="IWJ07_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAP9332262.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            35864..36646
                     /locus_tag="IWJ07_000662"
     CDS             35864..36646
                     /locus_tag="IWJ07_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAP9332263.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            36667..37374
                     /locus_tag="IWJ07_000663"
     CDS             36667..37374
                     /locus_tag="IWJ07_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAP9332264.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            37636..40434
                     /gene="ileS"
                     /locus_tag="IWJ07_000664"
     CDS             37636..40434
                     /gene="ileS"
                     /locus_tag="IWJ07_000664"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAP9332265.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(40483..42009)
                     /locus_tag="IWJ07_000665"
     CDS             complement(40483..42009)
                     /locus_tag="IWJ07_000665"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAP9332266.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(42024..42671)
                     /locus_tag="IWJ07_000666"
     CDS             complement(42024..42671)
                     /locus_tag="IWJ07_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAP9332267.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(42855..43184)
                     /locus_tag="IWJ07_000667"
     CDS             complement(42855..43184)
                     /locus_tag="IWJ07_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAP9332268.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            43361..44089
                     /gene="sfsA"
                     /locus_tag="IWJ07_000668"
     CDS             43361..44089
                     /gene="sfsA"
                     /locus_tag="IWJ07_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAP9332269.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            44105..45118
                     /locus_tag="IWJ07_000669"
     CDS             44105..45118
                     /locus_tag="IWJ07_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9332270.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            45118..46395
                     /locus_tag="IWJ07_000670"
     CDS             45118..46395
                     /locus_tag="IWJ07_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP9332271.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            46458..49160
                     /locus_tag="IWJ07_000671"
     CDS             46458..49160
                     /locus_tag="IWJ07_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9332272.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            49312..49629
                     /locus_tag="IWJ07_000672"
     CDS             49312..49629
                     /locus_tag="IWJ07_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9332273.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            49659..49979
                     /locus_tag="IWJ07_000673"
     CDS             49659..49979
                     /locus_tag="IWJ07_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP9332274.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            50087..51547
                     /locus_tag="IWJ07_000674"
     CDS             50087..51547
                     /locus_tag="IWJ07_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9332275.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            51615..51836
                     /locus_tag="IWJ07_000675"
     CDS             51615..51836
                     /locus_tag="IWJ07_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332276.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            51867..52049
                     /locus_tag="IWJ07_000676"
     CDS             51867..52049
                     /locus_tag="IWJ07_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAP9332277.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            52049..52462
                     /locus_tag="IWJ07_000677"
     CDS             52049..52462
                     /locus_tag="IWJ07_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAP9332278.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            52585..53766
                     /locus_tag="IWJ07_000678"
     CDS             52585..53766
                     /locus_tag="IWJ07_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9332279.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            54155..54228
                     /locus_tag="IWJ07_000679"
     tRNA            54155..54228
                     /locus_tag="IWJ07_000679"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:54189..54191,aa:Arg,seq:cct)
     gene            complement(54297..>54647)
                     /locus_tag="IWJ07_000680"
     CDS             complement(54297..>54647)
                     /locus_tag="IWJ07_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9332280.1"
                     /translation="INQKELFENFEIKNPHNFVFFNLKNGLVSSNAVSKYLRKKLKEL
                     GIEKQFTLHGLRHTHASILLYQGVNILSVSKRLGHSSLETTMSTYLHIVRELEDQDKE
                     KINTVFDSLYKNDN"
BASE COUNT        18038 a         9250 c        11783 g        15576 t
ORIGIN      
        1 gaagcaattt aattatctat aattattgat gttccgctga ttcttgtcat agtagctcag
       61 ctggatagag catccgcctt ctaagcggac ggtcgggggt tcgaatccct cctgtgacgc
      121 taaaaaaccg ttagaaactg aggtttctaa cggtttttct ttgttttttg tgccaatatt
      181 gtttgttttc aaaggcaaat aaaataaaaa aacatcctta tcgagtacaa ttataaggag
      241 caaaaaaata aaactcatca gaaaagagga agaagatatg acgatactat ctagaatttt
      301 agtgactcta gttgcaattg aattttttta tatcatgtat atcgaaacgg tgagaactga
      361 ttctgacacg acaagtaggg tttttaaaat gagtaaagaa gagttaagca gaaaatctgt
      421 tcaaacattg tttaagaacc aaggagtgta caatggacta ttaggagtgg gactacttta
      481 tggagcttat ctttcttccg catctaaaga aatcacaagt atgctattaa tatctatttt
      541 ttttgttgca ttatatggca gtttgactag cgatcgttgg attttactca aacaaggtgg
      601 gctagctctg ttggcattgc tttctatttt cttataaata tagaaaaaat aagaatttat
      661 ctaacaggaa actttgtgaa aaatattact ttatctcatg aaatatgata ttctaatgaa
      721 tggtatttta gaaaatttca tgaaaaagga gttagatagg tgttcaaaaa ttatgtttcc
      781 tcactaaaaa aagaattttc aggttatgat ctttcaagat ttcagaaaga tcttttggct
      841 gggatcacag tagctgctgt agcattgcct ttagccttgg cttttggggt atcaagcgga
      901 gctgatgcgg ccgcaggttt gattacggct atcattgccg gtatcgttat tggcggttta
      961 tcaggcggtt tttatcaaat atcaggtccg acaggtgcga tggcagctat cttgatgtct
     1021 attgcggcaa caaagggaat gcaaggtgta ttgcttgcta cttttttagc gggtctattt
     1081 ttacttgcag caggactttt ccgattagga acactgactt cttttatccc agcacccgta
     1141 attacggggt ttacttcagg tattgctatc attattgcat taggtcagat cgataattta
     1201 tttggtgttc actcggaagg tacaaacgtt attgaaaaac taagtagtta tcaaacgata
     1261 ggatttccga taaacacagc ctctttgatc atgggagggc tagtcatttt agggatgttt
     1321 atctatccaa aaaaatgggc gaaacgaatg cctagttcgc tgctggctat cattttggca
     1381 acagcagtta tgctattgtt ccatttacca gtagcaactg tgggtaagat tccacaaaca
     1441 ttgatcagta gtaatcgttt gaatatggga gattttagct tttctgcctt gcaacaagtg
     1501 gcagttccag ctattagcat tgctttatta ggaatgattg aaagcttgct atgtggtgct
     1561 tcggctggcc gaatggcaaa taagccctta gatagcaatc aagaattaat ggcgcaagga
     1621 attggaaatc tgttgcttcc tttctttggc gggattcctg ctactgcggc catcgcccgt
     1681 acaagcgtgg ccatcaaatc tggtgcacaa actagattag ctggtatgat acatgcagtt
     1741 gtcttactgc tctccatgct tgtttttgcg ccagtgatgt cacagattcc aatgccagca
     1801 cttgcaggtg ttttgattgt gacggcttgg cgtatgaatg aatgggaaac gatcaaggag
     1861 ctatttacta ataaatattg gtcagcagtt ctattgttta tccttacgat gatttgtaca
     1921 gttatctttg atttaagtat tgctatcgtg atcggggtga tcggcggctg tgtatttttt
     1981 gttgtcaaaa gcgctgctat cacgatttca ttagaaacga tcgattggca aagaatgggg
     2041 ctgccagaaa cggaaaagct gaataactgg acagtagtgt atattagtgg tccactgttt
     2101 tttatgtctt cagaaaaact caaaaataaa ttggaagaac taaatcatca cgatggtgtc
     2161 attttctcca tgcgaggcgt accaagtatt gatttgacgg cacgtgcagt ttttgatgaa
     2221 tttcaagaaa aagcagagca aagaggacaa acgattatct atacttcctt gcagccagaa
     2281 gtagaaaaac aactttctca cttgtgggaa catacattca cgcatcaaca tcaaacagtt
     2341 gctcatgcgt tgacagcttt acatgcgaaa tatactgcaa ttagccatta aaacaatgaa
     2401 aaagaacaga caagactgta aactgaagat tttatggtct tgtctgttct ttttaatatt
     2461 gctgtaaaag tgaagctagt acttgccagt cttgatggat aacaggtaca agggagcgat
     2521 tgtagaaaat agctgctgga tgaaaaagtg gaagaattcg atagtttttt tctgaccatt
     2581 cgtaggcatc cttttgttct gttaattgta agacaggccc tgtatagagt tgaccatgat
     2641 ttttggtaac cgtatattct ttgccaagta aacgttgaag accaatgttt ccaagtgtcg
     2701 caattaattt tggctggacc ttcatcagtt cgtaatccaa tattggtgca tgcgcaaata
     2761 cttcggaacg agtaggtgtc cgatttggat aaacagtttc tgactgttgt gtccgtttat
     2821 tgatccgatg agtcacgcga tacgggcggc tccttacagc actggtgata tacacctctt
     2881 ctcgtgttaa gccagttgaa gaaagtgcct gcatcaattc ttttccagct tgaccgctaa
     2941 aaggaacatg cgtatcaatc tctgttttcc ctggggcttc tcccacgagc atcagtttag
     3001 gatgaagagg tccttgccca gcgataaatc cttctacagg acggccgttc attcttaaag
     3061 aagcttcttg tatcagttct tttgggtatt ccatttatgc tcctcctttt tcttttatta
     3121 taagaataaa agaaacacaa tgcgactatt ttatttggta gaactgtgag gatagaaaag
     3181 cagaagtatt gcttgaattt ttgttttact ttttgatttt aaatttagtt gactaacggt
     3241 tgctgttctg gtaagctagt actgttgtaa ttgtggactc cacagctaca accgctcaga
     3301 acaagtaata agcacagcat atgctgtcac ttcggatggc gagtctaagt atatcagaag
     3361 gaggtgcttt atagcatgta cgcaattatt aaaactggtg gtaaacaagt aaaagttgaa
     3421 gtgggacaag caatctatgt tgaaaaactt gacgttgaag caggagaaaa agttgttttt
     3481 gacgaagtta tcttagtagg cggagaatct acaaaagtag gcgctccaac tgttgcaggt
     3541 gcaactgttg aaggtactgt tgaaaaacac ggcaaacaaa agaaagtcgt tactttcaaa
     3601 tacaaaccta aaaaacattc acaccgtaaa caaggtcatc gtcaaccata tacaaaagtg
     3661 gttatcgacg cgatcaacgc ttaatagcgt ttgagaaaga aggcctatat atgatcaaag
     3721 gaacatttaa gcgaaatgac gcaggtcaga tcgtttcatt cactttgacc gggcatgctg
     3781 atgctggtcc ttatggaagt gatatcgttt gtgcgggtgt ttctgcgtta gcgatcagca
     3841 ctgtcaacgg gattgcctca ttagccggtt ttgagccgat tgttgaaatg aatgaagaag
     3901 aaggcggtta tctttatacc gaagtcactt caggaatgac acaagaacaa aacaatattg
     3961 ctgagattct attagaaaat cttttattag gattacaatc gatagaagct gaaaactcag
     4021 aatatatcca aattaaaaca attaatgata aataggaggt gcagaccatg ttgttatcta
     4081 tgaatctaca attatttgcc cacaaaaaag gtggcggttc tacatctaac ggccgtgact
     4141 cagaatcaaa acgtctaggt gctaaacgtg ctgatggaca aacagttaca ggtggatcaa
     4201 ttctttaccg tcaacgcggt acaaaaatct atccaggtgt aaacgtgggt atcggtggag
     4261 acgacacttt atttgcaaaa gttgatggcg tagtacgttt cgaacgtaaa ggccgtaaca
     4321 aaaaacaagt gtctgtttat ccagtagctc aagaagctta attgaatctt atgattaaag
     4381 aggttgtgac aagacttttg tcacagcctt ttttgtttat aaaaataaaa gttgtagtgg
     4441 aggctacgaa gtttactttg tctttttaca cttaatattt ttttgccaat ttggaatcag
     4501 gatgttttga taactatcgt tttcaattga tggaagctat attgtttgct atcagtgaaa
     4561 taattatatg gtaaacagtg ttctttttct tgtttccatt ataaaaatcg tatcgggatt
     4621 tttcgttctt ttaaattgaa catttctatc tatccgcgtg ttatcaacaa aacaaaatca
     4681 ggtaaatagt tgacgaaaca gaaataaata catagtatta tgttttgaag cctaagtaca
     4741 atagtctata gaaaaggagc gataccattg gcaaaacgca ttgaccgaat ttatatgtat
     4801 gtcaaagaaa aaaccgctca tttaacaaag gcagaatatg atcaaggagt aacaacacag
     4861 gaaattgctg aagtgttagg gattcagcga acaaatagca gtaaagatct gaatcagcta
     4921 gtgcgcgaag gaaaactgat aaagacagac ggacgaccag tacgttacat atatcaaaca
     4981 ctcgttacgc acgtaaagcc agttacaaaa catgtagtca gttacaaaga aacgccggtc
     5041 tctgaagaaa aaaatacact ccaaatagat actaaggata tctttgcaaa aatcattgga
     5101 gctaacggca gcatgaaaaa ttcagtggaa caggcaaaag ctgcgatttt gtatccgcca
     5161 agaggattaa attgtttgat tacaggacct accggatcag gaaagactta ttttgcacac
     5221 gcgatgtatc attttgcaaa agcgcatcat gtaatagatg aggaaacgga actgattgtt
     5281 ttcaattgtg ctgattatgc caataaccct gagcttttga tgagtcattt atttgggtac
     5341 gcaaaaggtg cttttacagg cgcagatgaa gaaaaaatgg ggattatcga tcaagcggat
     5401 ggcggtatgc tgtttttgga tgagattcac cggttgccgc ctgaaggtca agaaatgatc
     5461 ttttacttta tggatcatgg aacttacagt cgtcttgggg aaacaacgaa atcccatgaa
     5521 gcaaatgtac ggatcgtagg tgcgaccaca gaagacccag gttcttctct attagaaact
     5581 tttgttcggc gtatcccaat caatatcaaa cttccggctt ttgaaaaacg tcctgcaaat
     5641 gagaaagtcg atttagtaaa aatcatgatt gcccacgaag caaaccgcac gcagcgaaag
     5701 atttctttga cagaagatgt agcaaaagca ttgatcggca gtgtgaccta tggaaatata
     5761 ggacaattga agtcaaatat ccagttagta tgtgcacgag gatttctaaa tcatatgcaa
     5821 tcacccgaaa tctcgatcac aatcgatgat ttgacagaag ggattcgtag cggattgatt
     5881 caattagcaa gcaaccgcag tgcaatggca gagctttcta aaatattgga gccaaagata
     5941 actgtagcac caaatgatac gatcatgaaa atccaatcgg actcctatga gttaccttat
     6001 aatctatatg atatcatcgg agataaagca gctttactga agtcggatgg tttggatcaa
     6061 gaagcaatca atcatttcat ttctacagat atcaacatcc atctgaagtc tttctataaa
     6121 gaccatggtt tttcttttaa tgcagacagc aaattagcag aatttgtaga tccgaaaatc
     6181 atagaagtga ccaatcagat ttatgtaatg gtcaaaagat ctttaccata tgagtttcag
     6241 caaaacttta tttatgctat gagcttgcat atcagttctt ttttgaaaaa aatcaactta
     6301 ggagaagaac ggcatacgaa tgataacatc cgtgagatgg cttttgattt tccagatgaa
     6361 tatgctgttg cgaaagaagt gcgtagttat atcgaaacgt attttcaagt ggagattcca
     6421 gaaagtgaag actattactt gaccgttcta ctcgtctcgt tacgggcaaa tcaagcaagc
     6481 ggcagaatcg gtgtagtcgt tgcagctcac ggaaacagca cagcaagcag tatggtccaa
     6541 gttgtccaac agttgttaga tgcagagaac gtacgagcag tagacatgcc tttggatatg
     6601 gatccgaaaa cagcattgga ccgaattgaa cgcaatgtac aagaagtaga tgaaggaagc
     6661 ggggtcatac tcttagtaga tatggggtcc cttgcgtcgt ttaattcgca aattcaaaga
     6721 gacactggaa ttccagtacg tactgttgat atggtaacaa cttctctagt tttggaaact
     6781 gtacgaaagg tctcagtatt agggacagat ctagatatgt tgcatgaatc attgaaaaat
     6841 tttcgaggtt acgcagaaat atcttctgta gaatctgttc aggaagcaaa acctattcat
     6901 aagaaaaaag ctgttttggc tatctgcgca tctggcgaag gtacagcaca aagaatcaaa
     6961 gaattgatcg aacgagcggt aaacaagcgt caagaaacag aattagaagt tctagctttg
     7021 tcgattgtgg aagtgaaaga agaactgcca gttatccaaa aagaatatca gatcatagct
     7081 actactggga ttactgattt gaatatccaa gccccattta ttcctttaga acgttttatt
     7141 gatcaaaata tcgaagtcat tttggatcag ttgctgatgg aatcagaatt ggaggaaact
     7201 gaattcatct ccctagatga agagtcagct aaaaatacgt gtgttgagtt tatctctgat
     7261 aacttcatct ttattaatgg tagtaagtta atcgatccaa tgtggcaatt ttctacacaa
     7321 atctctcaga cgacgggaat cggcgatgaa gaatatggat tcaaaatcaa cctagtgatg
     7381 cacacagcag gaatgatcga acgtattatt cggaatgaac cattgactgt ggaagaaaac
     7441 gaattgacaa atacaacgaa tgatcctctt tatagccaat tggcagcctc agttgttctg
     7501 ttggaagatc agataaaagt aaaagtgccg atagaagaaa tgtattatct gctacgtcta
     7561 gtccataatc agttagataa aaaagaatac actgtgccat gaatacacaa aaacgataca
     7621 cttttatagt gtatcgtttt tgtgtattgg ttaagaatcc tttaaattca attagtgtat
     7681 cgtaaaaaag ttggcacgct ttttgcttat agtagtgtgt gctaggaggt ggttgttcac
     7741 acagaaatga taggggtttc tgtgaagtag tgaggaagaa ggagttttgc agatggatat
     7801 tcgattggca cgtatagatg atcgtttaat ccacgggcaa gtcgcaacag tttggtcgaa
     7861 gcaaacaggt gttgaacgta tcattgtgat cagcgatgta gtcgctcaag ataaattgag
     7921 aaagtttcta ctaaaagaag ctgcaccgcc ggggatcaca gcaaatgtaa taaccgtgca
     7981 gaaaatgatt caaatctatc atagtggatt attatcacga gcaaaagtga tgcttttatt
     8041 caccaatccc caagatgttg aagcggttgt ccgctgcggt gtacagttga agtcgttgaa
     8101 tatcggcggt atgagtttta ctgaaggcaa atcaatgatt accaattttg tttcagtaaa
     8161 tcaacaagat attgattcct ttcgttactt gaacagtcaa gaagtggagt tagagattcg
     8221 aaaagtacct gctgacagga aaatcctttt gatggaacta ctagcgaaaa tcaaaaaggt
     8281 gtagcaagaa tggttgttgg aaaaggtcaa ataattctgt tgtaatggtt agcaattatt
     8341 aatatttttg aatttgttac aacaaaagta aataggaggt ataaatatgg tagggattat
     8401 cctagcaagt catggacaat ttgctgaagg aatcttacaa tccggttcaa tgatctttgg
     8461 cgaacaagaa aacgtcaaag ccgtgatttt gaaaccaagt gaaggtccag atgacttgag
     8521 agccaaattg gaagaagcgg ttgcttcttt tgataatcaa gacgaagtgt tattcttagt
     8581 tgatttatgg ggcggaacac cgttcaatca atcaaacaca ttattcgaag agcacaaaga
     8641 taaatgggca atcgtcagcg gtttgaattt accaatgttg attgaagcct acgcatcacg
     8701 cttttcaatg gaatctgctc atgaaattgc agcacacatc attgaaacag caaaagacgg
     8761 tgtaaaagta aaaccggaag aattagaacc ggcagaagca ccgaaagcag cagttgaaga
     8821 cgcacaacct aaaggagcac ttccagaagg aactgtggtc ggagacggaa aaatcaaata
     8881 tgtattagca cgtgttgact cacgtctgtt acatggacaa gttgcgacag cttggacaaa
     8941 agcagtgcaa ccaaaccgta ttatcgttgt ttcagatgca gtatcaaagg atgatctgcg
     9001 taagagacta atcgaacaag ctgctcctcc aggtgtaaaa gcaaatgtta ttcctattag
     9061 caaaatgatc gaagtagcaa aagaccctcg ttttggaaat accaaagcat tgcttttatt
     9121 tgaaaatcct gaagacgtat tgacagctgt tgaaggtggc gtagacatca aagaattaaa
     9181 tgttgggtca atggcgcatt cggtcggtaa agtcgttgta agtaaagtat tgtctatggg
     9241 acaagaagac gttgaagcgt ttgagaagct tgaacaaaaa ggtgtgaaat tcgatgtacg
     9301 taaagtacct aatgattcac gcgataacat ggatgatatt ttgaaaaaag caaaagctga
     9361 attagcaaaa gcgtaataaa taatattaaa atttatagga ggcttatcat gtctattatt
     9421 tcaataattt tagtcgtatt agttgccttt ctagctggta tggaaggtat tcttgatgaa
     9481 tttcaatttc atcaaccact agtggcatgt acattgatcg gtttagttac cggtaacctc
     9541 gaagctggta tcgtacttgg cggtacgctt cagatgatcg ctttaggttg ggcaaatatc
     9601 ggtgccgctg tagcaccaga tgctgcatta gcatcagtag catcagcaat tattttagtt
     9661 ttaggtggac aaggtgttaa aggtgttcct tctgcgatcg cgattgccgt tcctttggct
     9721 gttgcagggt tgttcttaac aatgatcgta cgtacaatcg ctgttccaat cgttcatttg
     9781 atggatgcgg ctgctgaaga aggaaacatc cgtaaggttg aaatgtggca catcattgct
     9841 gtatgtttac aaggggttcg tatcgcggtc ccagcagcag cgttgctatt tatcccagca
     9901 caaactgtgc aatcattcct tgaatcaatg ccagcatggt taacagatgg tatggctatc
     9961 ggtgggggaa tggtcgtagc tgtaggttac gctttggtaa tcaacatgat ggctacaaaa
    10021 gaagtatggc cattcttcgt tattggtttc gttgtagctg caatttctca attaacattg
    10081 attgctctag gtgctttagg tgttgcatta gctcttattt acttgaacct ttctaaaatg
    10141 ggtggttcat caaataatgg aggcggaagc aactctggtg accctttagg cgacattttg
    10201 aatgactatt aatcttgaag gaggaaaata aaatggcaga agaaaaaatc aaattatcaa
    10261 aaaaagatcg tttagctgtt gcgtggcgct ctaccttcat tcaaggttca tggaactacg
    10321 aacgtatgca aaacggtggc tgggcatttt ctatgattcc agcaatcaaa aaattataca
    10381 aaacaaaaga agaacgttct gcagcattaa aacgtcactt ggaattcttt aatacacacc
    10441 catacattgc ttctccaatt ttaggggtta cattggctct ggaagaagaa cgtgcgaatg
    10501 gcgcgcctgt tgatgatgta gcgattcaag gggttaaagt aggtatgatg ggtcctttag
    10561 ctggtgttgg tgacccagtg ttctggttta cagtacgtcc aatgctaggt gcactaggtg
    10621 cttcattagc tatgggagga aatatccttg gaccaatcat cttcttctta ggttggaaca
    10681 ttattcgttg ggcattcatg tggtatacac aagaatttgg ctacaaagct ggttctaaaa
    10741 ttactgatga cctttctggc ggattgttac aagacgtaac aaaaggtgct tcaattttag
    10801 ggatgttcgt attagctgcc cttgtacaaa gatgggtatc aatcaaattc tcaccagttg
    10861 tatcaactgt aaaattagat aaaggtgctt acatcgaatg ggataaattg ccttcaggtg
    10921 gtgaaggaat caagagtgca tttgaacaag ttaataatgg cttagccctt tctcctgaaa
    10981 aggtaacaac attacaagac aacttagacc aattgattcc tggtttagct gcattaggat
    11041 taacattgtt atgtatgtgg ttgcttaaga aaaaggtttc tccaatcgtt atcatccttg
    11101 gtttattcgt agtcggtgtt gttggacacg tcatcggact tttataatcc ataaaaatat
    11161 agctcgggcg ttgtcccgag ctttttttat atagtatact gttcataaac aaatatctgg
    11221 agggagaaaa tggttcaatc aatcaataca aaagtagact tggtaattga tgctacttcg
    11281 tttacgggtc tgacagatta cgggcaaatc atgataggtg ataatggttt tgagttttac
    11341 catgcgcgag atagacgcaa atatatccaa attccatggg aagaagtgga ttatgtacta
    11401 gcatcagtca tgctaaaggg aaaatggatt cctcgatatg ccattcaaac gaaaaaaaat
    11461 ggtacataca cattttcttc caaagaacct aaaaaagtgc ttcgtgcgat cagagaatat
    11521 gtagatgaag atagaatggt acaatcattg agtttctttg atgtagtgaa acgtggtatt
    11581 cgtagtatgt ttaaacgcaa gtaaaaaata aagaaaactt ctaagaggag gttttcttta
    11641 tttttttact ttgcagaaga aaatatgaaa tagcaaaacc aattatttgt cccctttaaa
    11701 tcccatatgc taaaattgat tagcaaagaa ggaggttata ttatgagttg gttatggaca
    11761 ttgattgttg gagccgtgat cggagcaatt gcaggtgcga tcaccagtaa aggaaattcg
    11821 atgggctgtg taacgaatat cattgctgga ctagtcggtt caagtattgg acaagcgatt
    11881 ttcggcgaat ggggaccaaa agctgcaggt atggcactta ttccatctat tttaggcgca
    11941 gtgattttgg tagcagttgt ctcatttttc tttggaaaac gatcataagt ttttacaaaa
    12001 tagtaaacta aaaggagttg cagcgagact gtgactcctt tttggcttta tttatttaaa
    12061 ataaaaagaa gttttccgga aatttttatt taactgtcgt gtacgaagaa aagtttgcta
    12121 taatggaaaa aagcgtgcaa agaaagaggg agtttcatta tgatgctacg tgtagaaaaa
    12181 ttgcgcaaaa aaatgcaaga agaaaatttg gattcatttt tggttaccag tccgtacaat
    12241 cttcgttact taacgaactt tacaggtacg acaggattag ctgttattac tttggaaaaa
    12301 gcatttttta ttactgattt tcgttatacc gagcaggcgg cagcgcaggc tcaagggttt
    12361 gaaattatta aaaatgtcgg acctattttt gatgaggtag caaacttagt acgcaaagaa
    12421 gaactgcact cattagcttt tgaggaaaca acagtctcat ttttagaata ttcggtttta
    12481 gaagaaatca tcgatgcgga attagtacct gtttcacaga tgatcgaaga actcagagaa
    12541 gtgaaagatg aagaagaaat cgctattatc gaaaaagcat gtcatattgc ggatatggca
    12601 tatgatcaca tcctgaaaat gatccaaccg ggcatgactg agattgaagt cgcaaatcaa
    12661 ctagactttt atatgcgttc tcttggcgct tcaggtgttt cttttgatac gatcgttgct
    12721 agcggattgc gttcggctat gccgcatggt gtagcaagca aaaaaatcat cgaacaaggt
    12781 gacttgatca cattggactt tggttgctat tacgaagggt acgtttccga tatgacacgt
    12841 acattcgcta ttggtgatcc tggggaaaaa ttaaaagaaa tttatcaaat agtcttagat
    12901 gctcaattag cagttattga tgctgcaaaa ccaggaatga ctggtgtcca attagatgca
    12961 gttgctagag attatatttc aaaacatggt tacggggaag catttggaca ttctacagga
    13021 catgggatcg gtttagagat ccacgaagga ccaaacgttt cttctcgtgc agaaaagcaa
    13081 tttgtaccag gaaatgtgat cactgatgaa ccagggatct acttaccagg aatcggtgga
    13141 gtacgtattg aggatgactt gttgatcaca gaagatggaa atcgtgtgtt gactcgttca
    13201 ccaaaagaat tgatcatctt atagtttcat caatagaaga agtaagcgta aaaatcagtg
    13261 ttcacagact ttgttataga aaatcactcg atttaatgat acactataga agaactttta
    13321 agctgtaaga gataataaag ttgggtgttt tctccaattc atagcagttt gcagctgatc
    13381 ggcggcagtt ttcttaccac gtgatcacta tgccgcatca tagagaattc ggatgtatct
    13441 cgtgcttagg caaagggagg aataaataaa tggcagatga aaaaaatttg attttaaacg
    13501 caaaccaaga tttaggagag atcgtgatcg ctcctgaagt catcgaagta attatcggga
    13561 ttgcagcttc taaagtagaa ggcgtatacg gaatgcgcgg aacttttgcc agcaatgtca
    13621 ctgaattatt aggacgtgca gctcacggaa aaggtgttta cttagcaaac gaggaagatg
    13681 gcttgaaagt agatctttac tgttatctag aatatggtgt ttcagttcct aaagtagcaa
    13741 tggaaatgca ggaacgggtc agccagcaag tccttttcat gacggatatc gatttatctg
    13801 aagtcaatat ccatgtagta gctgttgttc ctgaaaaatt agaacaacca acaatcgaag
    13861 aactttttga agacgaagag gaagagaatg aataaagaat tatcaagaca cgagattcgg
    13921 gaaatggcat tgcaggcttt atttccatta gacttcaatg cagacctaac aaaagaagat
    13981 gcgattttca acgcaatcga attagaccac cgcgacatga tcaacgaaga tgaatcagag
    14041 tttgttcctg tttatcttga tacgctagtt ggcggtgtct gtgcaaaaaa agatgaattg
    14101 gataaagtga ttgaaaaaca tttaaaaaac aattggcaca tcaatcggat ctcaaaaatg
    14161 gatttggtga tcttgcgtat cgcgattttt gaaatgactt atgtgacaga tgtaccagcg
    14221 gctgttgctt taaatgaagc aatcgagttg gcaaaaacat ttagtgatga ccgttcacgt
    14281 aaattcgtca acggtgtttt atccaatgta ttgaaagaat tagaagctgg ggcgtaagtc
    14341 ctagcttttt tctatgaatc atcataaacc tgttataaat cgggaaaatt ctatggtaga
    14401 atgaaaacac aaaaaatatc tgggagtggt tagatggcag aattgattaa cggaaaagaa
    14461 ttagctgaaa aaatgcaggc agagattgct gaaaaaatcc aaaaattgaa agataatggt
    14521 atccatcccg gattagtcgt tttattggta ggggaaaatc cagccagcca aatctatgta
    14581 agaaataaag aacgtgtagc aaaagaaatc ggaatccatt cattggtaga gcgttatcct
    14641 gagacgattt ctgaagacga actacttgca gaaattgaaa aatacaatca agatcctagt
    14701 tttcatggta tattggtcca attaccattg ccaaagcata tagatgaaga aaagatcctg
    14761 ttagctatcc gccctgaaaa agacgtagac ggttttcatc cgatgaattt aggcagactc
    14821 cttgcgggta aaccggacaa gattccttgt actccatacg gcatcatgaa aatgtttgaa
    14881 gcttattcta tcgatccggc gggtaaacga gcagtagtta tcggacgaag caatatcgtc
    14941 ggtaaaccga tggctcagct actcttgatg gcagatgcaa ctgttactgt tgctcattct
    15001 aaaaccgaaa atctggctga actggcacaa gaagcagata ttttagtcgt agcaatagga
    15061 cgtggacatt ttgtcacaaa agaattcgta aaaccaggtg ctgtagtgat agatgtaggg
    15121 atgaatcgtg atgctaacgg taagctgatc ggtgatgtga aatttgatga agtggagcct
    15181 attgcaggct ggatcactcc tgtgccaaag ggtgtagggc caatgactat cacgatgctg
    15241 atgtatcaaa ccgtaaaatc tgccgaagct tttggagcag gtgaatagat ggcacaagaa
    15301 tatttaactg tcactacatt aacgaagtat ttgaaaagaa aatttgatgc cgatccttat
    15361 ttagaacgcg tatatttaac aggcgaaata tcgaatttcc gtttacgtgc caatgcccat
    15421 caatatttca gcttaaaaga tgaccatgca aaaatatctg ctattatgtt caaaggagca
    15481 ttccaaaaat tacgattcca gccaaaagaa ggaatgaaag tccttgtcgt tgggcgtatt
    15541 tcattatatg aagcgggagg ttcttatcaa atctatgtag agcacatgga gcctgatgga
    15601 ataggtgctt tatatcaaga attggaagaa cgaaaagaaa agctgatgaa ggaaggcctt
    15661 tttcaagggc ctaaaaaaat gctgccacgt ttcccaaagc gaatcgcggt ccttaccagt
    15721 ccaagcggag cggttatccg cgatatcatc acgacggtga aaagaagata tccaattgtc
    15781 caattggttt tgttcccaac agttgtacaa ggaaaccaag cagcggacaa tgttgtcagg
    15841 aatatccaga gagtagaaga gatgggcaat ttcgatacaa tgatcatcgg tcgtgggggt
    15901 ggttcgattg aggatttatg gccgtttaat gaagagcggg ttgctcgggc gatttatcaa
    15961 gctaatacgc cggtgatttc atctgtagga catgaaacag ataccacgat cgctgattta
    16021 gtagcagatg ttcgggctgc cactccaaca gcagctgcag aattagctgt tcctgttttg
    16081 tcagaagagt tgatgcgcat tcaagaacgg caagctagat tgcagcaggc gtttgtacgc
    16141 cagatccagc gcaaacaaga acggttcgag cgtgctcgac gttcctatgt atttcggcag
    16201 cctgaaagat tgtatgaagg tcagaccatc aaattagacc agttgaaaca gcgattatat
    16261 caagctacac agcaaattta tcatgcgaaa gaaaaacaaa cgatcgcatt aagccaccaa
    16321 ttggaacaag tggctccgct ttatcgagtt aaagcagctc gccaagaaac tgcttatcta
    16381 gaaaaacgct tagaagagaa aatccaacaa tatatgaaac aacagcagca gcgttttgct
    16441 caagctgttc aatctcttga tttgttgagt ccattgaaaa tcatgggaag aggctacagc
    16501 tatacgacgt taaatgatca agtagtaaaa agtgtcaccg agattcatca aggggatttg
    16561 ctgacgatcc attatcaaga tggaatcgtt aaaggacaag tacaagagat agacaaagga
    16621 gaataaaaat atggaaaatc caacttttga agagtcactg caagaattag aaaaaattgt
    16681 catgcagtta gaacaaggag acgttccatt agaatcagct ctagattcgt ttaaacgagg
    16741 aatggaatta agcaaacatt gccaagatac attagctaaa gcagaagaga ccttgacgaa
    16801 aatgatgaca gaatcaaatg aagaagttga ttttgacgga aacgaggaaa gctgagatga
    16861 gattaactga cttttcagct attcatctac ctgctgtaga aaaagaaatc ttgtcttttt
    16921 tagatgagca tacaacggat agaggactct atgatgcgat gagttattca gtgaaagcag
    16981 gcgggaaaag aatacgtccc ttattacttt taacggccgt tgcttccttt gatgagccta
    17041 ttgatgttcc cgtctatcaa gtagcagctg ctttagaaat ggtgcatacg tattccttga
    17101 tccacgatga tttgcctgcg atggacaatg atgatcttcg tagaggaaaa cctaccaatc
    17161 ataaagtatt cggcgaagca ttagctatat tggccggaga tggtttgctt acaggagcat
    17221 tccagctaat cagtatggca cacttaggta attctcctaa gcttctgctg cttcagcaat
    17281 tagctgtttg tgcgggatca caaggtatgg ttgctgggca agctgcagat atcgagggcg
    17341 aatcgaaaaa gctttcttta gaagaattag catttattca tgaaagaaag acagggcatt
    17401 tgattcgtta tgccttgctg gctggtggca tattagcgaa gcaacctgaa gaaatcttat
    17461 tgctacttca acgattagcc gaacatttag gtttagcttt tcagataaga gatgatctac
    17521 tcgatgtgat tggtacaacg aaaacattag ggaaaacagc tggtaaagat gaacgtatgg
    17581 aaaaaaatac ttacccgcgt ttattaggcc tagaaaagac acgtgaggcg ttggagattg
    17641 agctgttatc cgccaacaag atcatagaca aattagaaga ggacgttttt tcttttgatg
    17701 gcgggctgat gcgccagatg atcaagcaat ttgatgtgga gaaatgaggg aaagaatgga
    17761 aaaagaacgc gtagatgtat tagcggtcaa acaaggatta tttgaaacaa gagaacaagc
    17821 caaacgttca gtgatggctg gtttgattta caacgaaaaa aacgagcggt ttgataaacc
    17881 gggagaaaaa attccagtga gtagtgaatt aagagtcaaa ggtaaaaaac ttccttatgt
    17941 ttctcgaggg ggattaaaat tagaaaaagc actcaaagca tttgatctat ctgttgaagg
    18001 aaaaacattg ttagatattg gtgcatccac tggcggattt actgatgcag ctttgcaaaa
    18061 tggcgcaaaa atgagttatg ctttagacgt tggctacaat cagctagctt ggaaaataag
    18121 acaagatcct agagtcgtcg taatggaaag agtgaatttt cgttatgcaa aacccgaaga
    18181 ctttacctta ggaattccag aagttgctgt tatcgatgtt tcttttattt cactaaaact
    18241 gatgctgcct ccattacatg ctattttaaa agaagaggga gaagtgattg ctttaatcaa
    18301 accccaattc gaagcaggca gagaagctgt agggaaaaac ggaattgttc gtgaccctca
    18361 gacccatcaa aaagttttag aagacatttt gtcatttgcg gcacaaggtg gatatgatgt
    18421 gctggaatta agttattctc cgatcacggg gggagaagga aatatcgagt tcttggctca
    18481 cttgaggaaa gtacccgaaa gcggaacaat caattcagct attaatatgg cagaagtggt
    18541 ttcaaatgca cacgaacagt ttgaccataa ataaattgta actgtgaaaa tcaagtgaga
    18601 agtatgcgga ttcttcttta ttataccaag gaaataagct atcattgatt aagaagaata
    18661 tgctaaggag cgaagacgta atgagaaaaa aagatcggca tcgtttgatt actcgattgt
    18721 tgagtgagca agatatccga aagcaggaag aatttgttga aatcctgaaa agcaaaggaa
    18781 tatctgttac tcaagctacg atttccagag atataaagga actgaaactg atcaaggtac
    18841 cggcttcaga tggcggctac cgttatagca taccagctga aacaaacgaa gatgtaaata
    18901 ctaaattgga aaaactatta aaagacgctt tcgtggcagt tgaccaaatg gaaaaatttg
    18961 ttattttgaa aacattgccg ggaaatgctt cggcagcagc aaatttgatt gataaacgtt
    19021 acaagaaaga attattttcg attatcaatg atgacgataa tgttttgatg attacacgaa
    19081 ctgaagacca ggcagtttta ttgaagaaag acttcctgca ttacctttaa gttgaggagc
    19141 gaatgtgatg ctacaagaaa tcagtattac taactttgcg attattccgg aattgcggct
    19201 ttcttttcac gaaggaatga ccgcgctgac cggagagact ggtgcgggta aatccattat
    19261 tatcgatgct ttaggtctgt tagcaggagg aagaggttca agcgattata tacgtcaagg
    19321 agcagaaaaa tgtgtgcttg aagggttgtt tgaactacca aaacaagaag gattctctga
    19381 actgatggtt gaattaggga tcgaaacaga tgaggacaat ctgattgttc gacgggatat
    19441 gtccttaact ggaaaaaatg tttgtcgagt gaatggtcat attatcacat tagctaatct
    19501 gagaagaatc ggcagttatt tagtagatat ccaaggacaa aacgaacatc aggaactgct
    19561 ccagcctgaa tcacatcttg ctttattgga tcgttttgga gatgtagctt tccaacagaa
    19621 gaaaaagagt tatcagcaag aatatatctc ttatcgtgag ctggaaaaaa gggtacgaaa
    19681 agtgcagcaa aatgaaaaat cctatgttca gagaatagat atgctccatt tccagcaaga
    19741 agaaatagct gctgctgatt tgcaagtcgg agaagaagag aaattaaaag aagaaagaga
    19801 aaaacttagc aattatcaaa aaattgctga tggattagct gcaggttacg gagcactgac
    19861 gaataatgaa caaaacagtg tagatggtgt tggcttggcc gtttcagaac tgcaagggat
    19921 cgcccatcta gatgtggagt atgaagcaat ttatgaaaat atccaaagtg cttactattt
    19981 gctgcaagat gcaatcggcg atatgagtcg tcagattgat ttattggagt tagatgagag
    20041 tcgtttagaa gaagttacgc aacgtttaga attaatccga cagttgaaac gtaaatacgg
    20101 cgaatccatt gagtcaatat tggcttacta tgacgaaatc accgaagagc tagcttcttc
    20161 tgatttttca gaaggtcaat tggacaaaat gaaagaggaa ctagagcaaa aagagcttct
    20221 acttcagcaa caagccgcag atcttcatga agcgcgtaaa aaaatcgcaa aagaattgga
    20281 aaaatccatt ctgcatgaat tgaaaagtct gtatatggaa aacactgagt ttgaggtacg
    20341 tttcttaaaa gaagaaaata gacaattgaa tcatgatgga tttgaccaaa tcgaatttta
    20401 cattacgacc aatccaggag agccactgaa gcctttggtg aaagtcgctt ctggtggcga
    20461 actgtctcgg atgttgcttg ctttgaaaac tattttttct tctgagcaag gtgtgacaag
    20521 tattattttt gatgaagtgg atacaggtgt gagcggaaga gttgcacagg ccatcgcaga
    20581 taaaatttct caaatatcga aatactctca agttttgtgc atcactcatc tgcctcaagt
    20641 tgctgcagtc gccgactatc aatattatat tgtgaaagca gtgattgatg gacgtacgca
    20701 aacttctgtt tcagagttga aaacaaaaga aagagaagaa gaaattgcac gtatgctggc
    20761 tggaagtgag attacaaaac tcacactcga acatgcaaaa gaactattga aactcgcaaa
    20821 aaggacctga gccacctcgc agtggttcag gtccttttca gcagctgata gctatatttt
    20881 atttttacat gacactcatt acagcagtca atacaacaga tccaatcgtt aaaatcagca
    20941 ttgtccaaat gacaaatttt gttactttag caaaagtact tgtttgtttt ttttcacgca
    21001 ttgttttcaa cctcgtcatt aattagttga ttcaagtgta caatcttttt tccatttcca
    21061 caagagggct tttttataaa aacttccctt tttttaaata ataggtagtt acgatacaaa
    21121 taaataatgt taatagaact agccatataa aggcatcatc cctttcagca ataggtaact
    21181 taatgttcat tccatagata ctgccgacga ttgtcggtat agttaaaata atcgttaaag
    21241 aagttaaaat tttcataata ttattcagat tattcgatac gattgctgaa aaagtctgat
    21301 tcatttgttc tactagtttt aattgaatgc gagaagtagt cattgcctgc tctgtttcca
    21361 ccaatacatc acgtaaatga tttttcaaag catgattttc ttggaaaagt ttattttgcg
    21421 ctagttcttt caatgcatct aaattagcag aagtcgcttc tctgaaataa acgaggcttt
    21481 tttggatatc catgatttga taaagttgtt tgttttcagt ggatacttga agtttggctt
    21541 ccaaagtatc gcattgagat tgaagcgtag ctaaataact gttgtaagag agtgtgacct
    21601 gccaaatgaa ttgaaagaag atattgagat taggcgaaat atcacttttt ggaaaggtct
    21661 ggctaaagac tttctttaaa aacagtggtt catgattcgt aattgtgata agcttctttt
    21721 tgggcgtcaa aataatagag agcgggtatg tatccacttg aaaataaccg ctaggacttg
    21781 ttttagcaaa aggatattgt aacaataata acgctggctt tgtcaacttt gattgattga
    21841 attcttctgt acgggaattt tctgcatcgt ccaatatgga agtgatgtaa tctgacggaa
    21901 tttcaaattg atgtgtcagc tgatgaatct cttcttgatt aggacgttct acacaatacc
    21961 agatcgtttg gtcagtttcc tttccggtat tacgcagttt ctcattatct aatgtaaaat
    22021 atttgatcat gattttcctc ctgttgcctg tttcttttat tataacggat agggaattgg
    22081 aatacatgga atgtgtttgt aagatataga atataactgc ataaatttaa ataaaatgaa
    22141 acagttgtgt aaagtatata cagtatactt gaagcaacga taaaaaagga gtttacgttc
    22201 atgggcacat tcacaccact tatcttgttt tggtatattt acccggtaat cgttttgttt
    22261 ggttgccaat ttcttgtaaa attattttcg ttaaatcgtc gttttaaaat aaaagcccct
    22321 gatttggcga ttcctttttt gtggggcggt atgcatgcat tgtctagaaa tacagggaca
    22381 atttctatct taccgttttt ctttatttct gttttgctaa tgggaatttg tattgctgtt
    22441 ttccaagcat attattatga agaaattatt tatccgcgtt atttcaagat gacttggcgt
    22501 ttggtttttc tgttgacaat cgtcttgtac gctgtcttga tcattgtgaa catactttct
    22561 tacctatgaa aaaaatagaa aagctgtaaa aagtctttta aaaggctttt tgcagcttga
    22621 tttttaaaaa aatgtttatg aataaaaaaa gttgttcccg gatttcccag aaaaggtggt
    22681 agaaagtggg ggattgtggt agactaattt tagttagtgg aggaatgggg ggctttcaat
    22741 gttgatgggt gaattccaac ataatattga tgcaaaagga cgtttaatcg tgccctcaaa
    22801 acttcgtgaa gaacttggcg aaaagtttgt tctgacacgc ggattggatg gctgtctttt
    22861 cggctatccg atgagcgaat gggaaaatct ggaagctaaa ctgaacgaaa tgccgttggc
    22921 taaaaaagac gcacgtactt ttgtgcgatt cttctattcg gctgccactg aatgcgaact
    22981 tgataaacaa ggacgaatca atatacccag cacgttaaga aattatgcag ctttgacaaa
    23041 aggttgcgta gtcatcggtg tatctaaccg aattgaaatt tgggatgaga cacgatggca
    23101 agatttttct gcagcagcag aagagaactt tgacgaaatt gctgaaagta tgattgattt
    23161 cggactataa gaataggaag aggctgacta atgactgaaa catttcaaca ctatacagtc
    23221 atgttgaaag aaacggtaga tggattgaac atcaaaccgg atggcgttta tgtagactgt
    23281 acactcggcg gtgccggaca tagtgaatat ttattaagcc aactaagttc taaagggcat
    23341 ctgtatgcgt ttgaccaaga tcaaaaagca atcgatcacg ccaagctgcg tttagcttct
    23401 tatgtcgaac aaggtatggt cacatttatc aaagcgaact tcagagaatt agaagaacgc
    23461 ttgagtgagc atgtagataa ggtagacggc attttgtatg atctaggggt ttcctctcct
    23521 cagctagatg aagcagaacg tggatttagt tatcatcaag atgcaccgtt ggatatgcgc
    23581 atggatcagt cagctccttt atccgcttat cacgtaatta atgaatattc ctaccatgaa
    23641 ttagtaaaga ttttcttccg ctatggcgag gagaaatttt ccaaacaaat tgctcgtgaa
    23701 atcgaacgag tacgaaaaac aaaaccaatc caaacaactg gtgagcttgt cgaaatcatt
    23761 aaacaagtca tcccagctcc cgcaagaaga aaaggcgggc atcctgcaaa aagaattttt
    23821 caagctgtgc ggattgcagt aaatgacgaa ctcggtgccg aagaggcctc tttagaacag
    23881 gcaatccggt tattgaaagt caatggaaga atcagcgtaa tcacattcca ttcattggaa
    23941 gacagaattg tcaaaagtat gtttaaagaa tatagtacgg tacaagatct accaccggga
    24001 cttcctgtca tacctgacga attccaacca gaactgaaag tcattactcg taaaccaatc
    24061 cttccttcag aggaagagct aacagaaaac aatcgatcac gcagtgcaaa gttacgtatt
    24121 gcagaaaaaa taagaatgaa agaggagtag aatgatggca gaattaaaga aaatgcaaga
    24181 ttacgaatat gatttacctg aattggtgga tcagccggaa caacaaatgc ctgattcttc
    24241 ttcgaatcaa gaaatcattc ttccacaatc accggcaaaa cgattaaagc agatttctgc
    24301 tttggaaaaa attgctgtgg caagcgtggc gattgctatt gttgctttat gtgttttaac
    24361 agttatgctg cgcacaaata tcagcggagc tgaaaaagaa atcacgtcaa tacaaacaga
    24421 aatcaatgat aaaaagcaag aaaaaacgag tttagaacaa gaaaaaaacg aactttctcg
    24481 tactgaacgg attaaacaaa tcgcagaaga aaaaggattg tctattaatg acgacaattt
    24541 aaggaaagtg aagtaaatga gtcttaaaaa taaaattcgg cggtttatgg aaaagaaaaa
    24601 tctaaatccg atgaacaatc gcaaaaaagt aggtatcatt ttatttgcta cgagtattgg
    24661 attgttcttt ttatttgccg tccgtttctc gtatatcgtt attggaggtc atgttgctgg
    24721 tacctcatta gctgaaaaaa caaaacagtt gtaccaagga agtgaagtag taaaagcaaa
    24781 aagaggaacg atttatgatc gtaacggtgt ggcgctcgca gaagatgcct cctcttattc
    24841 aattaaagcg atactctcaa aaacatatac ctctggggat aaaaaactgt atgttgaaga
    24901 aaagaatttt gataaaatag cagaaatcct gcataagaat ctttctatag acaaaaaaga
    24961 tgcactgaat attttagagg acggcgcaaa aaaagagctg tatcaagttg aatttggtag
    25021 ctatgggaaa aatatctccc aagaaaccaa gcaaaacatc gaagcggaca tgaaaaaaga
    25081 aggtgtcgct ggtctttatt ttgtcgacca tcaggctcgg atgtatccaa atggcgtctt
    25141 ttcttctcat tttattggat acgcagttcc agacaaagat gagaatggct tagttggtaa
    25201 actaggtttg gagtctgctt acaatgatat cttgagcggt aaagatggta agattattta
    25261 tcaaaaagat aattttcaaa atcctctgcc aggtacagtg gcagaagaag aaaaagctgt
    25321 cgacggacaa gatatctaca cgactttaga cagcagacta caaagttatt tagaaacact
    25381 gatggatcaa gtaaatgaag aatatcagcc agaagaattg acagcagtcc tgatgaaagc
    25441 aaaaaccgga gaaatcttgg caatgggaca acgacctact ttcaatcctg aaacaatgga
    25501 aggattgact ggaaaagatg ctatctggcg taatttcttg gtacaagata gttatgaacc
    25561 aggatcaacc atgaaagttt tcactactgc agctgctatc gaagaaggag aattcaacga
    25621 gaatgaaacc ttccaatctg gaaaaatcca agtagaagat gctacgatca atgaccatga
    25681 ctttggtgaa aaaggtgtgc tgaccatgcg tcaagctctt tcatggtcaa gtaacgtcgg
    25741 gatggtcata ctggaacagc gattaggcgg acgatggtat aactatttac aaaaactggg
    25801 atttggacaa agtactcatt ctggattaga tgatgaagta aacggtgctt tacctacatt
    25861 aaatattgtc gaccgagcaa tgagtgctta tggacaagcg gtaggtgtga cgaatttcca
    25921 aatgatgaaa gctttcactt ctattgcgaa taacggtaca atgattcaac ctcgatatat
    25981 cagcaaagtg gtggatcccc aaactggaga agagcgaaca actcaaacag aagttctagg
    26041 gcaacctttt tcaaaagaaa caacagaaaa agtccgtgaa tatatgagag atgttgtcga
    26101 aagcgaaaac tacggtagtg cttatggtgt atatagtgta cctggataca atgtatcggc
    26161 aaaaaacgga acagcccaaa tcgcttctga tacaggggga tatcaaacag gagatacagc
    26221 ctacctgtat tcgattgtgg agatggttcc ttctgaagat ccagactatg tcttgtatct
    26281 aacaatgaaa caccctaaga catatgatcg aatggcattg gctaaaatcg caaatccatt
    26341 gatgaaacgg gcaatggatt ttaaagaaac tgaagaagat tcagatacag aaacaaaaac
    26401 agaaaaagta tccgtagccg attatcgtaa tcttgaagcg gacgttgcag cagctgatgc
    26461 tcaaaaaagt ggactgcaac ctgtagtgat cggtaatggt aaaaaagtac aaaaacaatc
    26521 tactgccaac ggtgatcagt tgatctctgg agaaaaactg atcctatata ctggtggtga
    26581 taagctgatg cctgacgtaa caggatggtc aaaagcagat atcatgaagc tggggaaaat
    26641 cctcggtatt gaagttagtt ttgatggaga tggttattgt gtcaaacaag aacttgctcc
    26701 atatgaaaaa atcactgaag acaaacttag ttttacttta gaagaataag aagagaaata
    26761 ggagagaaaa gagcatggaa tggacacaag cattgattcc cattgtcagc agttgtgcaa
    26821 tgacaattgc cgcaatgccg ctatttattg gttattttca aatgaaaaag caaggtcagg
    26881 cgattcgtga ggagggacca aagtggcaca atagcaaagc cggaactcct acaatgggcg
    26941 gcttagtgtt tctgatcggc tctattttga caggaatttg ggttggagct tggcaaaaac
    27001 agctaactcc tactcttttc atcttgcttt ttgtattagc gttatacgga gtaatcggct
    27061 ttcttgatga ttttataaaa atattcaaga aaagaaacat gggactgaac tcgaagcaga
    27121 aattgattgg acagattcta gggggaatca tcttttatct tgtttaccgt tctgaaggtt
    27181 atccaggtgt attgaatttc ttcggactag atctaccttt aggaatcatt tatggtattt
    27241 tcgctctctt ctggcttgtt ggattttcaa atgcagtaaa tctgacagat ggcatcgatg
    27301 gattagttgc tggcttagga agtatctctt ttgcagctta cggtctaatt gcttggcatc
    27361 agcatcagta tgatgtatta atcatttgct taagtgtttt aggaggttta ctgggtttct
    27421 tcgtctacaa cagaaaacct gccaagattt ttatgggaga tgtcggttca ttagcgttgg
    27481 gcggactttt agcggcaatt tctataatgc taaaccaaga atggacattg cttttgattg
    27541 gtttgatcta tgttatggaa acagcaagtg tgatgcttca agttacttcc tttaagctta
    27601 caggaaaacg aatttttaaa atgtctccaa ttcatcatca ctttgaaatg tgcggatggt
    27661 cggagtggaa gattgataca gttttttggt tgatcagcat tgtgacttcg ttgatcacat
    27721 tatggatcgt ttggtaggag atgacgggaa atgaaaaaaa tatcaacata tgaaaataag
    27781 aaagtgcttg tcttaggact cgcaaaaagc ggagtaagtg cggccaaatt attacatgaa
    27841 ttgggcgcac ttgtcaccgt taatgatgga aaaccttttg acgaaaatcc ggaagcacaa
    27901 gagctgcttt ctttagggat aaaagtaata acaggtagtc atccaatcga attgttggat
    27961 gaagaatttt cattgatggt caaaaatcca gggattccct acagtcatcc tttagtagca
    28021 aaagcccaag aaatgggtat tccggtaatt acggaagttg agctagctta cgaagtagcg
    28081 gaatgcccaa ttattgggat tacaggaacg aacggaaaaa caacgacaac aacgatgaca
    28141 ggactactac tgaatgcagg agcagatcaa ggaattgcac gtttagcggg taatattggg
    28201 tatccagcaa gtggtgtagc gcaagaagca aaatcagaag ataaaattgt gatggaattg
    28261 tctagcttcc aactcatggg aatcacagat ttccgccctc atatagcggt gattaccaat
    28321 atctatgaag cacatattga ttatcatggt acaagaaaag aatatgtcaa agctaaatgg
    28381 aaccttcaaa aaaacatgac agaaaaagat tatctgatcc ttaactggaa tcagtcagaa
    28441 ctacaagaat tagcgcaaag aacaaaagca cgcgtactgc ctttctcgac aaaagaagtg
    28501 ttggaagacg gtgtgtatgc tgacgattac tcaatatact ataaaaaaga aaaaataatg
    28561 gaaatatctg aacttggcgt accaggtaag cataatgtag aaaatgcact agctgctatt
    28621 tctgtagcaa aattgtatgg tatatcaaat gaagctatac gagaaacgtt gcattttttc
    28681 catggcgtac cacatcgtac gcaatatgta ggagaaatcc aaggcagaaa attttataat
    28741 gattcaaaag caaccaatat tttagcgact aaaatggctc taagtggatt tgaaacgtct
    28801 aaggtcgtgt tattggctgg cggattagac agaggaaata catttgatga attaattcct
    28861 tcattaaaag gaatcaaagc aatggtcgta tttggccaaa caaaagaaaa gttgatggat
    28921 gctggaaaaa aagcaggcat cgagacaata gtaacagccg attctgtaga acaagcagtt
    28981 cctttagcat tagaaaacag tacagatggt gatgtagttt tattatcacc agcgaatgcc
    29041 agctgggatc aatacccaaa ttttgaaact agaggaaaca gattcatgga agctgtgaac
    29101 cgattaaaat agaaaaagta gaagcgagcg ataaaaaatg aaaattttgg ttacaggcgg
    29161 aggaacaggg ggacatattt atccagctct tgcctttgtc aattatgtaa agactcaaga
    29221 accgaatgct gaattcatgt atgtgggagc aaaacgaggt ctagaaaaca aaatcgtccc
    29281 tgatacagga atgcctttcc ataccttgga gattcaagga tttaaacgaa aaatttccat
    29341 gcataacatc aagacggtcc gattgtttct taagagtatc cgtcaagcaa aaaaaatcct
    29401 cagtgatttt caaccagata tcgttatcgg tactggagga tatgtttccg gagcggtagt
    29461 gtacgctgca gccaaaatgg gtattccaac agttatccat gaacaaaaca gtgtaccagg
    29521 tgttaccaat aaatttttaa gccgatatgt ggatcggatc gctctttcat ttaaagatgc
    29581 ggcttccttt tttcctgaaa acaaagcagt tcttgtaggt aatcctcgag cacaagaagt
    29641 agcagatact aaaaaatcag atattttgaa aacatttgat ttggatccag aaaagaaaac
    29701 agtcttgatt tttggcggta gtcaaggagc acttaaaatc aatcaagcag tatcagtttt
    29761 tttaagtgaa tatattcctt cttcttacca agttttgtat gcttcaggag atagatatta
    29821 tgaagagatc ctaaataaaa taaatcctga aatacaaaat attagtgtcc agccttatat
    29881 caaaaatatg gcagaggtca tggcttccag tgatctgctg gtggggcgtg ctggtgcaac
    29941 ttcgatcgca gaattcacag ctctggggtt accggcagtg ttggttccca gcccatatgt
    30001 tacgaatgat catcagacta aaaatgcaat gagtcttgtt cacgcaggag cagcaaaaat
    30061 gattgcagat aatgaactaa caggtgaatc actaagtcag acagtcaacg aaatcatggg
    30121 tgatgaagaa ttgcaaaagc aaatgtgccg agcttctaaa gaacaaggaa tccctgatgc
    30181 ttcaaaacgt ctttatgact tagtaaaaca aataatcaaa aaataaaggc ggtggtctta
    30241 gtgagtaaga aaaaagaacc aaaaaaattg tctcatccgt cttctgatga aaacaatttg
    30301 actccttggc aaaaggctaa ccgaaaatat ctggcagaac aagagatgaa aaaccaagag
    30361 ccaaaaaaag aagacataac attagataca cccctttcag attctgaaga agaaaaaacg
    30421 gttcaagagg aacagcaaga agaaagccag tccgagaaaa aaatcgaatt ttttgaagag
    30481 atcaagcaaa agaaaaacgg tggaccatat aacggttctt ttttgaaccg gctgcccaat
    30541 ttaaaaagtc aacggcacaa agtactattt cgtcggttag ctttgatcat cactgtattg
    30601 ggtataccgc tgattttttt gatctattat gtttctcctt atagcaaatt acaagcagtt
    30661 gcagtatcag gcaataagac agtcaacagc caagaaatca tctctgatac aaaattgtca
    30721 ttaggagaaa atgtttgggg acagtatttt catcgatcta cttatatcga tcgccttaaa
    30781 aaagcacagc caagaatcga aacagcaaat atacatttca aagggatgaa tgaattcgat
    30841 ttggatgtaa cagaatataa agagattgct ttgatcgcaa aaaacaatca atactaccca
    30901 gtgattgaaa atggaacagt attagatgaa aaagtagcaa atcctacaaa aaatctccct
    30961 atcttggaag agttcaaaga ctctgcaaaa atcaaagaat taacaaagca atacaatcag
    31021 ttatcatcag aattacaaaa agcaatttct gaaatcaagt acacgccaag agcaagcaat
    31081 aaaaatctga tccaattgaa tatgaatgac ggcaatcaag ttattgtaaa tatcaataat
    31141 cttgcgaacc aaatgaaata ttattcgcaa gtagccaaag atatggatga aaaaggtgtg
    31201 atcgacatgg aagttggtat cttctcgcat ccttacggta gcactcaaga aacggaagaa
    31261 tcgggccaat cggaagaaac aactgaaagt gaagaaaatg cggatcaaac aaacgaatca
    31321 agtgaagagg cagaaacgca aaaagcacaa acacctggat cgtctagcac aaacggatca
    31381 tctgattcaa atgattcaag tgaaacaagt caaatagaag ataatccaag tacaactccg
    31441 tcaagtttaa atgaaaattg ataaaacaac tttaaaaagt aagaatgtac tttctaaaaa
    31501 ccagtaattt atggtagaat tggaagcagt gaatatatct attaataagt atattttaaa
    31561 acggacgcta gttgttcgct tgtatatgga atagtaagta ggagggaatc ccattcatgg
    31621 caaaaacagg aatgtatgta ggccttgata ttggaacgac atctgttaaa gttgtcgtgg
    31681 ctgaatatat cgacagccaa atgaatatta ttggagtagg aaacgcaaaa tcagaaggga
    31741 ttaaccgagg cattatcgtt gatatcgata aaacggtcca agcgatacaa cgagcagtac
    31801 gacaagcaga agaaaaagca ggtatccaga tcaaaggcgt aagtgtcggt ttgccagcaa
    31861 acatgcttga agtagaaaat tgccagggaa tgattgcagt taacggagat tcaaaggaaa
    31921 tcaccgatga agatgtcaga aatgtggctt cagctgccct tgttcgttca atacctcctg
    31981 aacgtcaaat cgtgtcaata ttgccacaag attttactgt cgatgggttc gagggaatca
    32041 aagatcctcg tggaatgatc ggcgtaagat tagaaatgta cggtctgttg tttacaggac
    32101 ctaaaacgat catccataac atacgtaaat gcgtggaaaa tgccggatta atcgtcaacg
    32161 aaatggtgat tactccttta gcgctgactg aatcaattct ttctgacgga gaaaaagatt
    32221 ttggtacgat cgtcattgat atgggtggcg gccaaacaac aacggctgtt atgcatgata
    32281 agcaattgaa gtttaccaat cttgaccaag aaggtggcga atttgtcact aaagatatct
    32341 ctattgtatt aaatacgtca tttaacaatg cagaagcgtt gaaaataaac tacggggatg
    32401 catacccaga acggacatca ccagatgaag aatttccagt agatgtgatt ggtcagtcag
    32461 aaccagtgaa agtcgatgaa cgctacttgt cagaaattat ttcagctcga atggaacaga
    32521 ttttcaacaa agcaaaagat gcattggacc aaatcgaagc attagagttg ccaggtggcg
    32581 tcgtattgac aggcggagct gctagtcttc ctggtgttgt ggacttggca caagaaatct
    32641 tcggcgtaaa tgtaaaattg tatgttccaa atcacatggg gctgagaaac ccggtattta
    32701 caaatgtaat aagtatcgtg gattattcag caaacttgag tgaagtgtat caattggcaa
    32761 aaattgctgt tacgggtgaa acctcagcag ctcgtcaaat ggtagttgaa caagaaacaa
    32821 caaacacata tgagagctat gaagcacctg aagaaacaat ctacgatgag ccggaaccaa
    32881 aagagtccgg agaaaatgta aaaaataaaa tcaagggttt ctttacaaat atttttgatt
    32941 aattcatcct gggaggaata agatatggaa ttctcaattg ataataatat caatgacggc
    33001 gcagtaatca aagtcatcgg agtcggtggt ggcggtggaa atgctgtaaa ccgaatgatt
    33061 gaagaaaacg ttaaaggtgt tgaattcatt acagccaaca cagacgtgca agcattaaaa
    33121 aattcaaaag ctgaaacagt tatccaatta ggccccaaat atactcgtgg tttaggtgct
    33181 ggttctcagc cagaagttgg acaaaaagcg gcagaagaaa gtgaacaatc cttacgcgaa
    33241 gctttagacg gtgcagatat gattttcatc actgcaggaa tgggtggagg aaccggtaca
    33301 ggtgctgcac caatcgttgc aggtatcgcg aaagaattgg gcgcattgac tgttggtgtc
    33361 gtgacacgtc cattcacttt tgaaggaccg aaacgtggcc gttttgctgc agaaggtatc
    33421 gctcgtttga aagaaaatgt ggatacatta ttaattatct caaataaccg tttattagaa
    33481 gttgtagata aaaagacacc gatgcttgaa gctttccgtg aagcagataa tgttttacga
    33541 caaggtgtac aaggtatctc tgatttgatc acagcgccag gttacgttaa ccttgacttt
    33601 gctgacgtga aaacagtgat ggaaaaccaa ggtacagcat tgatggggat cggtgtggct
    33661 agtggagaag atcgcgtagt agaagcaacg aaaaaagcaa tctcctctcc attgcttgaa
    33721 acatctattg atggagctga acaagtactt cttaacatca caggcggttt ggatatgaca
    33781 ttgttcgaag cacaagatgc ttcagatatc gtagcaaatg cagcaactgg cgatgtaaat
    33841 atcattctag gtacatctat caatgaagaa atgggcgatg aaatccgtgt gacggtcatc
    33901 gcaacaggta tcgatgaatc aaaaaaggaa cgcaagtcta gccgaccagc tagacaagca
    33961 caaatgcaat cacctgctca aaagactgta ttagatatgg atcaagctaa accaacttct
    34021 tcagaagaag aaaacagttt tggcgattgg gatatcagac gtgaacaaaa tgttcgtcct
    34081 agagttgatg attccaattt tgacaatatc gaaaaaaaag aatttgatac attcaaccgc
    34141 gaagaaacaa aatctcatgg cgatgatgaa ttaaatacgc caccgttttt ccgtcgtaaa
    34201 aaataaagga gaggaagagc acatgattgc tgacaacttg cagaaaatca agcaagaaat
    34261 tcaggattcg tgtgctcttg ttcatcgtga tccgaaagaa gttacattag tagctgtaac
    34321 aaaatctgtt gaatccgctc aagctgaaga attggttaga gctggcgtta tgaattgtgc
    34381 agaaaaccga gtagataaat tattagaaaa gaaacaagat ttatttgatt ttcctgaaat
    34441 aaagtggcat ttaattggta atttacagcg cagaaaggta aaattgattg taaatgaaat
    34501 tgattacttt catgcgttgg acagtttaag attagcagaa gaaatccaga agcggttaga
    34561 gaaagaatta tcctgtttta ttgaagtaaa tgtttctggt gaggaatcaa aacatggaat
    34621 ttcaccagat gagcttttac cgtttgtgga atctttagct gcctgcacaa aaatcaaggt
    34681 tgttgggttg atgactatgg cgcctaaaga agcttctgat gagcaaatcc ataaagtgtt
    34741 tggtacgctg aaagaattgc gtgatcttgt tcaagcaaaa ggttatgccc atgcaccatg
    34801 tagtgaatta agtatgggga tgagtcaaga ctttcaaatt gcgattgaag aaggtgcaac
    34861 atttatccgt gtaggaacag cgctattcaa agacgaataa aggaggaaca aaaatgccgc
    34921 ttttaaacag aggagcaatt gctaattttt tcggattagc tgatgaggaa gagtacgaat
    34981 accaagatta tcctgaaaca acaaaaaagg ctgtacagcc aactaaaaaa aatacaacac
    35041 agaatcaatc aactcattct gtatctgctg cgaatactca tgaaaaacaa aaaacggcga
    35101 cttcacgtcc tgtaacgcat gaaagtattc agccggctga aaagaaaaaa gtaattgaac
    35161 tacatcaatc acctacaaaa actgtagcga aagaaagaac agcttcaaga acctctacac
    35221 aacctaaagc aaatcttgct ggaaaaatca ctgtaatgga accaagagct tattctgaag
    35281 ctatggcgat tgctaaacga atcattgctg gagaagcaac cttagtgaat tttcatttag
    35341 tagaagaaaa acaagcgaga agaatcgttg attttctaac aggaacagtt tatgcactag
    35401 atggagatat caaacgtgtt ggagatgaga tttttctttg tacaccgact gggatggaga
    35461 ttgacagttc aaccgctcaa tcttttgcag atgcgaattt gtttgatctg taagaggagg
    35521 aactacgatt tatactattg cactattact tcatttactt caacgggcag cctatttcta
    35581 tagcattatc ttgatggtat atgcattgct ctcttggttt ccaggcgggt atcaatcagg
    35641 gctaggacga ttattagcta agatctgcga accgtatatc agtttgttcg atcgtctgcc
    35701 tttgaggatt ggacccttgg atttcacaat tttaggggca atcctgatct tgaacttagc
    35761 cgtacaaggt ttagcacaga tcgttatatg gataactgtc catttagttt attgattgaa
    35821 gcaacaaaat ctttattctc agaatagata ggaggggaaa attttggatg caaatgtata
    35881 ccagcacttt cgggcagatg aacgtacatt tatcgattcg gtaggcgatt ggattgaaca
    35941 ggttcaagga caatatgcac cctatctgac cgaatttctt gacccaagac aatcctatat
    36001 tttagaaaca ctgattcgac aaagcagtga tttgcgtttc atgttttatg gcggatatga
    36061 acaggcagaa agaaaaagat gtttgatttt ccccgattat tacgaacctg tagaggaaga
    36121 ttttgaaaca gcattgtatg aaatccatta tcctagcaaa tttgccagcc taagtcatgg
    36181 gaaaatcctt ggaactttag tgggaacggg gatcaaacgc tcttactttg gcgatatcat
    36241 ctcggacggt gatcgctggc aagtatttat tgcaaatgag gtggatcatt ttgttgctgc
    36301 acaagtaaca aagattggaa aggttgctgt acgcttggaa ccaatcaaat acacagaaat
    36361 gattatgccg aaagatagct ggacacaaga aagaggaaca gttagttcca tgcgactaga
    36421 cagtgtgatc tctgagattt ataatatttc cagacagcgg tccaagcagc tgatcgaagc
    36481 tggtaaagta aaagtgaact ggacagaaaa tacaagacct gattttgtac ttgaactatt
    36541 agatattatt tcagtcagag gttatggcag agtccagatc caagaacttg aggggaagac
    36601 gaaaaaagaa aagtacaggg tactgttcgg tgtattacgt aaataaaata aaaaggtggt
    36661 ttactcatgg ctttaactcc attagatatc caaaataaga cattcccgac aaaaatgcgg
    36721 ggatataatc aagacgaagt agatgatttc ttagatttag ttgttcgtga ctatgaggaa
    36781 ttgactcaaa gaaatcgaga attagaaaaa gcagtcaaac attctgaaga aaaacttgag
    36841 tacttcaatg aattaaaaga tgctttgaac caatcaatca tcgtagctca agatacggct
    36901 gataaagtaa aaacaagtgc aagcaaggaa tccgaagtga tcgttacttc tgcacaaaac
    36961 aaagcagatg agttagttgc gaacgctgaa aaacgtgcac atcaattaac aactgatgca
    37021 gaagaaaaag caagaaaaat cttgacagat gcaacagaaa aagcaagaca gcttgcgaca
    37081 gaaactgaag atcttaagaa aaaaacacga gtattccatc aacgtatcag cttgatgctt
    37141 gagtctcaat tagaacaagt gaaaagtccg gaatgggatg aaattttaca accattctcc
    37201 agctacgtga ctgacagcca tgaagtgatc aaagaagttt tagcaaaaca gcttgacaat
    37261 gaaaatgata ctgatgtaaa ctcagacaca ggagtcatag aagcacctat gacggcattt
    37321 gatacacagg ctattgatat ctcgatcgtg cctggtgaaa atgaaaacga gtagaacaga
    37381 aaagcacttt gttattctta aagcgagccg atgagagtgg aagttcggta agacactgca
    37441 tggtgaaaga tcctctactt gagacctgct tttccgaaat tagagtaaga agcagcggct
    37501 gacctcgtta ttggtcacag agagtttgca ctagatagat gcaactaaat ttgggtggta
    37561 ccacggcatg ttcgtccctt gcggctgaac atgctttttt gtgtccggaa aacaaataaa
    37621 ggagtgtatg gttgaatgaa aatgaaagaa accttgcaat taggaaaaac aaaattccct
    37681 atgcgtggta atttgccaaa ccgagaaggc gagtggcaaa aagaatggga agaaaatcga
    37741 atctaccaaa aaagacaaga gttgaacgaa ggaaaaccta gctttgttct tcatgatgga
    37801 cctccatatg ccaatggtaa tatccattta ggacattcat tgaataaaat cagtaaagac
    37861 attatcattc gttcaaaatc aatgtctggc tatcgtgcac catatgttcc aggatgggat
    37921 acacatggat tgccgattga acaagtattg gctaataaag gcgtgaaacg taaagaaatg
    37981 tccatggcag aatacttgga aaaatgtcgt gaatatgcac tttcccaagt agataagcaa
    38041 cgagcagatt tcaaacgcct aggagttgca ggagattggg aacatcctta tgttacatta
    38101 gatccttctt atgaagctgc acaagtacgc gtatttggta aaatggctga aaaaggctat
    38161 atttacaaag gattgaaacc aatttactgg tctccgtcaa gtgagtcttc tttagcggaa
    38221 gctgaaattg aatataaaga tgtaaaatct ccatctattt ttgtagcttt caatgtcaaa
    38281 gacggcaaag atattcttga tgaagataca gcttttgtca tttggacgac aacaccatgg
    38341 acattgccag caaaccaagg tattgctgta aatccaaaat ttacgtatgt attggtggaa
    38401 gccgatggcc gtaagtttgt cgtagcgaaa gatttgctag aaacagtcca atcagaaatc
    38461 ggttgggaaa atgtaacagt tctaaaagaa tttgctggac aagaaatgga atatatgact
    38521 gcaagtcatc cattctatga acgtgaatca cttgtgattt taggcgatca tgttacttta
    38581 gacgctggta ctggattagt ccatactgct ccaggacatg gggaagatga ctatatcgct
    38641 ggtagcaaat ataaactatc tgtagtgtca cctgtagact caaaaggatt gtttacagaa
    38701 gaagcacctg gatttgaagg tatcttttat gacaaagcaa atcctatgat cactgatcta
    38761 ttaaaagaaa aaggagcttt gctgaagcta gatttcttta ctcatagtta tcctcacgat
    38821 tggcggacga aaaagcctgt gatctatcgg gcaactccac aatggtttgc atctatcgat
    38881 aaattccgtc agaatatttt ggatgaagta gaaaaagtag agtgggttat cccatggggt
    38941 aaaacacgtt tgtataatat gatccgtgac cgcggcgatt gggtaatttc ccgtcagcgt
    39001 gcatggggag taccacttcc gattttctat gctgaaaatg gagaagcaat catcacacca
    39061 gaaacaatcg aacatgtggc aaaattattt gctgaacatg gatcaattat ctggtttgaa
    39121 aaagaagcga aagaacttct tcctgaagga tttacccacc caggttctcc taatggcgaa
    39181 tttacaaaag aaaaagatat catggatgtc tggtttgatt ctggttcttc tcatgaagct
    39241 gttttacgtc agcgaccaga attatcattc ccagcagata tgtatttaga aggatcagac
    39301 caatatcgtg gctggttcaa ctcaagtatc acgactagtg tggctatcaa tggtgtagca
    39361 ccatacaaat cagttatttc tcaaggattt gctttagatg gtgaaggccg taagatgagt
    39421 aaatctttag gaaacatcat tgcaccagaa aaagtgatca aacaatttgg tgcagatatc
    39481 cttcgcttat gggtatctag tgtagattat gaagcagatg ttcgtgtttc tatggatatc
    39541 ttaagtcaag tggcagaagt atatcgtaaa atcagaaata cgatgcgttt cttattagcg
    39601 aatacagaag atttcgatcc taaaaaagat actgtttcgt acaatgattt acgttctgta
    39661 gacaaatata tgaccgttcg attgaaccaa gtaatcaaag aaatccgaga agaagggtac
    39721 gacaaatata acttcatgca tatttaccgt acagttatga acttcttgac ggttgatcta
    39781 tcgtcattct acctggattt tgctaaagat gtagtttata ttgaagcaga agatgattat
    39841 caacgtcgtt gcatgcaaac tgttttctat caaacagctg tagctttgac aaaattattg
    39901 actcctatca ttccgcatac agcagaagaa atctggtcat tcctgaaaga agaagaggag
    39961 tacgttcagt taagcgagtt tcctggttat gaagagtttg caaaccaaga agaattgatg
    40021 gatacctgga ctgctttcat ggacttccgt gataatgtct taaaagcatt ggaagaagca
    40081 agaaacagca aattgattgg taaatctttg gaagcaaaac taactgttta tccaaaacaa
    40141 caagttcgtg aaatgttaaa agctttggat gcagatattg cacaattatt gatcgtttca
    40201 ccagactact ttgaagttat ggcagcagat gaacaagtgc cagataacgc tatggtattt
    40261 gatgatgttg ctattacagt ggaaaaagca gacggggaaa cttgcgatcg ctgtcgccaa
    40321 gttcgtaaag atgtaggagt agatgaaaaa cttcctcatc tatgcggtcg ttgtgcaaaa
    40381 atcgttgaag acttctatcc tgaagctgta gctgaaggat tcgaagaaaa gtaaaaggga
    40441 aaagggggtg tgacatgttc tttgtcacac ccccttttgt tatcaatcgt ttaattttcc
    40501 acgttcacga taccagttat ctggttgcca gatccattcg aatccgtctt tttcaagtag
    40561 ttcgaaagct gctttaggtc ccattgatcc agctttgtag ttgggaaaat ccggttgtgt
    40621 ttggtcccaa gcttttcgaa tgacatccac gatatgccaa gattgagcga cttcttccca
    40681 atgagagaag ttcgttccat caccattcaa cgcatctaat agtaattttt cgtaagcttc
    40741 tgggctattt tcaaccattt ctgcactatt tctaaattcc aatttgattg gttccgtttc
    40801 aaatccttgc cctacttctt tcccattcaa ggataaagaa aatccttcag ttggctgaat
    40861 atagatcgtt agaacattag gtggaagtgt cgtatcgtca catggttcat cgacagatgt
    40921 tttgaataca ttaacaggaa cttgtttgaa tacgatgttg atccgagtcc ctttttcagt
    40981 caaacgtttg cctgtacgta catagaaagg aacacctgac caacggaagt tatcgatagt
    41041 gaatttgcct gcgacgaatg tttcagtctc tgattgagca tccactttgt cttcttcgcg
    41101 atagcccttg aattgtacac catccaattc accttgagca tattgcccgc gaacgaagtt
    41161 ttctaacact tcttcttcgt tataaatgcg gatagctttc aatgctttga tcttttctgt
    41221 acggatttct ttttcggaaa atgctactgg cggttccatt gcaagtaatg ctacaacctg
    41281 taaaatatga ttttgcacca tgtctttaag tgcgccacta tgatcgtagt agcccccgcg
    41341 atcttctaca ccgaggtttt cgccaaaagt aatttggata ttgtcgatat agcgattgtt
    41401 ccattgagcc tcaaaaatat tattggcaaa acggatagct gagatgtttt ggatcatttc
    41461 ttttcctaaa taatgatcaa tcctgaaaat atcttgttct gggaatactt ctctgatttg
    41521 ctggttcaat tcaaaagcag actggtaatc agaaccgaac ggtttttcta tgatcaaacg
    41581 attgaagcca ttttttgtca taatattttg tgatttcaaa tgagaaacga tcgttccaaa
    41641 aaattgaggt gccatagcca aataatagac acggttaccg tttaattgat agtgtccatc
    41701 taatcggtca gacaattctt ttaatgtctg ataatgcgtt gaatcattta catcatggga
    41761 ttgatagtaa aaatggcttg caaaggtagt tgcttcttca tctgaaggat tcaatgcacg
    41821 gattgtttct ttgacaactt cacggtagtg ctcatcgctc cagggacggc gagcggttcc
    41881 gattacagca aaatgctcgc ctaaatttcc ttttctgtaa aggcggaaca aagacgggta
    41941 caatttacgc tgggctaggt cacctgtacc gccgaatatc gtaaacaata catttttttc
    42001 attagacatg tggatacact cctttatatt ttatcgacaa aaatcgtgct ggccgcctta
    42061 aagctgacgg ggattttctt tctatcattt tggatagtga tcggtccttc ataagctgcg
    42121 atgtcaacga tctcatattc ttcgttgata ttcaggtcaa tagtgactaa gtaatccaac
    42181 aattcttttt catcaagcac tcgtgcgata cgcacttttg taccgacagg ataggactcc
    42241 aatgtttgtc ggcgttcttc atggacaact tcctcttttt taggaatcga tcctccatga
    42301 ggacaatgtt caggatgatt caaatagttt tctaaatgat ctgcaagatg ttccgaagtc
    42361 acatgttcca atacttcagc atcatcatgg acttcattcc agctatagtc taaatgttca
    42421 actaaaaaaa cttcccataa gcgatgtttg cgaatcagac tgctagcttt ttgcagtccg
    42481 cttgcagtca attgtacacc ttgataaggg gaatgttcta caagctcttc tttcacaagt
    42541 ttagaaatca tttcactgac agatgcagct gagacatcta gactagatac gatttgttta
    42601 ttgttgactt tcgttgtatc tccacctagt tctaaaatga ttttcaaata atcttctcga
    42661 tttggtgtca tattcgatca tcctttcatg gtaaaaaacc atcattagta agtgtaacaa
    42721 aaaaaacgtt aattgactat ttttctaaat aaaaaacaaa ggatttttag acgtttttac
    42781 aacaaaaaac gaaagatgat agagaagact cattctttcg tttataaagg atatatatat
    42841 acattgggag ggatctattt ttcagataac aacttatatg aagtgtctgc agattcgtca
    42901 taaagagttt gttcgggata agaaaagata agataaaaac catactgatc acgtacgact
    42961 aaacgatatt catctatttc taccacagat gtgccaagag actgaccgtt gatcaaaaac
    43021 gtgtaatcag atgtaatctg taattgttgt ttttgctggc tatcactaaa gaaccatgta
    43081 ccttgatact ttgagagtcc ttctgacttt tccgcttgtt ttttcttttt caagtaaata
    43141 aaacttgaga gaacggtaat gagtgaacca agaaaataga tcattttttt tatcaccaaa
    43201 tcactccttt atgagaaaat aatcgcatag acatattctt tcttctttat ttcattgtac
    43261 cgaataaaag ctaaaatttc tctaagactt tcgactggtt tttttactgt ttttctacaa
    43321 attatgatag gatacatagt aggaagaaag gtgacataat ttgaagtatc agcaagtgga
    43381 aatagctttt tttattgacc gcccgaatcg gtttatagca cattgtatga aaaaagacgg
    43441 agaaataatt attgcgcatg tcaaaaatac aggtagagga aaagaggtcc tgataccagg
    43501 cgctgaagtg gctattgtat ttgctcctgg acctaaacgg aaaaccgcct atgatctgat
    43561 tgctgtaaaa aagaagggtg actggtttaa tatcgatagt caattgccta atcggctggc
    43621 aattgatggt attttagacg gcacgatcct tttaccagga ttaactggtc catttacggt
    43681 atacaagcgt gaagtaactt atcttcactc caaatttgat atttatttag agacggtggt
    43741 cggacaaaaa gcatttgttg aagtcaaagg tatgacactt gaaaataaag cgattggtgc
    43801 ttttcctgat gcgcccacac tcagaggatt gaaacatata ggagaattaa cgtatgccgc
    43861 acaagatggt tatgccgctt atgtcttgtt tattgctcag ttcgaacatc ttcatctggc
    43921 aacgatccat gaagaaatgc aaccagcact agctgatatg gttcgacatg cgcagcaaag
    43981 cggagttcaa atcctcgcct ataattgtca agtatcggca gatgaggtaa ctgtcaagca
    44041 ggccattcca tttgatgtag atatcccttt tgaagatcct atacaataga aagaaggtaa
    44101 aataatgatc aacttaggta ttatcggaac aaattggatt actcatcaat ttgtccaagc
    44161 agctttaagc acaaaaaaat atgaattgac agcggtatat tctagacatt tggaaaaagc
    44221 acaggaattt ggttctcatt atgaagggga tatcgaattc gcaacagatt tgaagacttt
    44281 tttcaatatt actcatatgg acaccattta tattgcttct ccgaatagtc ttcactttag
    44341 tcaagctaag caagcaattt tggcaggaaa aaatgtgata gttgaaaagc cagctttttc
    44401 gacgccaaaa gaaatggatg aaatcatcca tttggcaaat gaacacaatg tgtatttttt
    44461 tgaagcagca agaaatatcc atgaatcagg ttttaaaaag attgccgatt acttaccatt
    44521 aaaagacgag ataatcggtg caaatttcag ctatatgaaa tattcttcgc gctatgacca
    44581 agtattagat ggagaagaac cgaatatctt ctcgactcat ttctcaggtg gtgcattgat
    44641 ggatttaggt gtttaccttg tatatgcagc tgttgcttgg tttggaatgc caaaagaagt
    44701 ccattatttc ccaagaaaga tttctactgg tgtagatggt cttggatggg ggatcttacg
    44761 ttatgagaat tttgatgtat cgatccagcc aggtaaaatc ggtgattctt atttaccgtc
    44821 agagatttat tttgactcag gaacgctagt gatgaatgga gtcaatgcaa ttgaacaagc
    44881 acaatttcat gatcgcgcgc ataaagaaat cattgatttg gcggtaacag ctgcagaaaa
    44941 tccaatggca gatgaagcag aggattttgc caatgtcatg gctcatccga aagatccagc
    45001 ttggggattg ttgtatgaag agtggatcga gctagctaga aatgtgaacc aagtgattta
    45061 tgatttaaga aaaaatggcg gcatcaaatt cgatgcagat aatgaggaag atttttaatg
    45121 aacgaatttg tacaagtatt tcccgaaaat tggcagaaaa aatatgagga taaagggttt
    45181 atctcacctt ctcttattca acgagcagtt tttcagccac taagtaataa acaaagtata
    45241 gtagcggttt ctcctacagg ttcaggtaaa acactggctt atctttggcc tctactacta
    45301 aacgtggaac caggtgaagc aagtgcgttg gtcatttttg cttcttctca agaactagct
    45361 attcaagtag cagatgttgc acgtgaatgg ggaaaagaca aagagttgaa agtccaatct
    45421 ttggtgggcg gagccaacgt caaacgtcag attgagggtt tgaaggaaaa accagaaatc
    45481 ttgattggta caccaggaag aatattggaa ttgatgaaag caaaaaaaat aaaagcacat
    45541 caagtgaaaa caatggtatt tgatgaagca gaccagctat ttgacgctgg gaacagccaa
    45601 atcattgatc aaattcttca tcaagctcca acagagtatc agctagcttt tttttcagcg
    45661 acagcagatc gttcccttga aagcattgaa aaaatcactg ggaaaacgtt tgagacaatt
    45721 gacgtgacag cagaggacga ttctcgaaaa ggactgagac attacttttt acgtgtaccg
    45781 atacgaaaga aagatgacta cttgagacgt ctcacgcata tagaagattt tcatggtctg
    45841 attttcttta atcaactgac tgaattaggc gtaatggaag aaaaattgct gtatagaggt
    45901 gtaccggtag gaagtctggc ctctgatcag aacaagctac tccgaaaagc agcgctagaa
    45961 caattcaaaa agaaaatggt cagagcactt ttgaccacag atgtggcggc tagaggttta
    46021 gatattacag gtcttcctta tgtagtgaat gcagaagttc cgttaagtga agaagcttat
    46081 ttgcatcgtt cgggaagaac tggtcgaatg ggtaatgaag gaatagtaat tacactcgta
    46141 caagataata atctaagaga tttaaaacgg cttttacgtc catcaaatat aacacttgaa
    46201 gagatttttt tatacggagg taagctgcaa actgagcagc caaccaaaga agatgcagct
    46261 cctgcagtaa ataaaaagta tgcgaaaact ttttctggaa aggagcagaa cccagaatat
    46321 aacaaagaaa aaataagaaa aaaagttaaa aataaatcaa aaaaagagag aaataaagga
    46381 gcaaggcgca aataagcgcc tttttatgct atgattatga aaacgggaac aaaattggtt
    46441 gccagaggag gggttatgtg aaagaacgtc aagaagagat cttgcagcta ttgacagaaa
    46501 accaacaagg attgacagca agtgatgtag ctgagcgctt aactatcgat cgaagcaatg
    46561 caagccgata tttaagtgaa ctatacaagg cccatcatat tgtaaaaacc gctggccgac
    46621 cagtcgttta ctctttgcca acggaaaagt caaaatctga tgaagtgcat gtcgactctt
    46681 ctacgcaagt gacttttgaa acactcgttg gagaaaatga ttctttaaaa gtcagcatac
    46741 agcaggcaaa agctgcgatt ctttatccac cgcgggggtt gcatacgatt atttttggag
    46801 aaactggtac agggaagtcg atgtttgctg aatgtatgta tcattttgca atcgattcag
    46861 agatgctttc tgcagatgcg ccatttgttt ctttcaattg tgcggattat gctcaaaatc
    46921 cgcaactgct ctttggacac atctttggga tcaaaaaagg ggcttacaca ggagcagctc
    46981 aagatagtcc cggtctaata gccaaagcag acggcggtat attgttttta gatgagatcc
    47041 accgtttacc gccggaaggg caggaaatgc tgtttacttt cattgataaa ggtatttacc
    47101 gtccgcttgg agaaagtgca caagtacatg aagcatctgt tcagatcatt ggggcgacga
    47161 ctgaatcttc agaaagtttc ttatctacgt tcaatcgacg gattcctatg gcaatcacct
    47221 tgccgaatct tgcttcacgt tcactggatg agcgttatga aatcatctct ctttttatca
    47281 aacaagaagc gaatcgtttg aatcaacgta tcgatatcga aaaagaagcg attcttgcct
    47341 tcatgcttta tgatgcggaa ggaaatatcg gtcaagtgaa acgagacttg aaactagtct
    47401 gtgcaaaatc ttttttacat taccgtactc atcatgagaa aaagctaatc attcgaaaag
    47461 aagaactttc ccttcaagta caaaaaggat tactgaaaat caaggaaata ccggaacgtc
    47521 tcgaccgatt catggacagc aaaagtcagt atttgacatt tgaaccagga tttgctgatg
    47581 ttgtctggtc acaagatcca gaacgaaaca tgcaggtcta taacgatatt gaggaaaaag
    47641 tcacttctct ttccgaaacc ggtgtggaga acattgatct agagaccctc atttcaaaag
    47701 atgtggatgc ttatttccag acatatgtca aagaattgac taaaagttcg attcccaaag
    47761 atcttttgcc agatgaaatc tggcggttaa ccaatcaatt gtatgatgtg gctgaaaaac
    47821 ggttagaccg tatttataat gaaaaagctc gttttgcttt tgctttgcat ctgcaaagta
    47881 cattggatcg tgtgagagaa ggtcatatga tcgtccaccc agatttgaat aacgtacgga
    47941 aaaattttaa gagggaattc caggtagcaa tcgatctttc aacgatcatt gaagaagaag
    48001 aacaagttga gattcctttt gatgaaatcg gctttattag tatgtttctt tctatcaatc
    48061 taggagaaag tgagcctgtt aaagaaaaca aagtagatgt tctcatattg atgcacggca
    48121 gacagactgc ttcaagcatg ttggagatgg cacaagaact attaggaaca aaagtaggga
    48181 aagcttttaa tatgccactg gttatggaag ttcaaacaat gtatgaagaa gtgttacaat
    48241 atgtcaaaaa ctatcaagag catttgacga atggcctact cttgttaaca gatatgggat
    48301 cattaaacac cttcagtgat ttgatttatg aagagactgg cattcggacg aaagctatca
    48361 cgatgacaag taccatgatt gtgatagaag cgatacggat ggcaagtgtt ggaagaagct
    48421 tagaagatat ttatcaaaat atccagcttt cctttgaatc gatcgtacgt gatcagttca
    48481 aagcggaaaa gtctgaaact ttacagcgta aaaaagccgt aatcgtgaca tgctttactg
    48541 gtgaaggagt agctgccaaa ttatatcagc gtattatgcc agtgattaat caaaataagg
    48601 tagaactgat ccagatgcag tttttagaac gtgaatcatt caaaaaacac atcgatgcct
    48661 tattggaaga atatgagatc aaagcaattg cgggaacagt tgatgttgat tatcaaaata
    48721 tcccattttt ttcagcctat gacgtttttg atgatgaaaa actaaatatt ttaaagcgta
    48781 tagctgatga tgagatcccg acagctaaaa ttgtcgattc acttgctgga tcattaagcg
    48841 atgtcttttc tattgatgaa ctgatccgtt cattgcaaaa gacagttcag cagatccaga
    48901 ccgatttgtc gattatcgtg gaacctggag tagatactgg aatcgttatc catttagcct
    48961 tcatgttaga cggattgatc aaacaagaaa gccatcggca atttgagaaa ctgtctattt
    49021 tctcaaaaat ttatcgtttg gaaatggata tcgttcgggc aaacttgatg gcactagaac
    49081 gaaaatatca ggtaaaaatt ccagaagatg atatcgcatt tcttacacaa atgtttcttg
    49141 aaaataaaat aaaaaaatga acacactaat catactgttt acacactaaa catctgtgtg
    49201 cgcactataa aaaagagcta aaaagcatga aaacgctatt tcagctcttt tttttattgg
    49261 catgcttctt gcgttatata aaagtgtaaa aaaataaaag gagtgttaaa aatggcaaaa
    49321 aaaacaatca tgttagtttg ttcagcaggg atgagtacga gtttattggt cacaaaaatg
    49381 caaaaagcag cagaagaaaa agggatggaa gccgatattt ttgcggtctc tgcctcagat
    49441 gcagataata atttagaatc taagaatgtc gatgtattat tattaggtcc tcaagtacgt
    49501 ttcatgaaag ctcaatttga acaaaaactt gcgcctaaag gtattccatt agatgtcatc
    49561 aatatgcaag actatggcat gatgaacgga gaaaaagttc ttgcacaagc agaaaatcta
    49621 atgaaataaa tcaagtatgc agttgggggt ataacaacgt ggaagatcaa caaaatctag
    49681 aagccatcat ggggttaatc atgtttggcg gtaatgcaaa gagcgatgca atggaagcga
    49741 ttgcggctgc taaaatcggt gattttgagt tagcggatag gaaaatcgct gatgcagaag
    49801 aatcacttat acaagcgcat catgcacaaa cagacatgtt gacacaagaa gcacaaggaa
    49861 atcatatgca agtgacatta ttgactgttc acagtcaaga tcacttaatg acatctattg
    49921 cgtttactga tttagcaaaa gaaatcatcg aactttatcg tcgcgtatac agcgaataaa
    49981 tacaacttct tttttgaacg gaagaagaaa aatttttgaa cggatttttt attttgttat
    50041 aaggagaact ctctttatat aaaaaactta taggaggaag taaaaaatga acggactatc
    50101 aagttggatg gagaaataca tcctgccggt tgccgcaaaa atcggagcgc aaaaacattt
    50161 agttgctttg cgtgatgctt ttatcggcac actgcccgca acaatggcgg gatcagtcgc
    50221 tgtaatgatc aatgcaatta ttcgtgattt accgcaacag tttaacagta cctatgctga
    50281 cagtttagca gcagacaaag gaatctttgc cgtattgaat gtcatcatcg gaatcaatgg
    50341 ttttgtctgg aacggtacac tagcaatcgc cggattgatc tttgcctttt cttgggggta
    50401 taatcttgcc cgagcttatg gagtaaatga tctagctggg gggatcgttg gtttagctac
    50461 cttgattcaa ggtatttcct ttggtccagc attatcagca gcattagatg tgaatgttcc
    50521 ttcaaatgtc gcagatgcaa tcaatagttc tgagattggt gcaacgattg cagatggcac
    50581 actgtcagtt ggattatggg gttggctaaa actagatcat ttgaatggaa acgcttattt
    50641 tacagttatg atcatgggag ctatttcagt cattattttt tgtaaattga tgcttgccaa
    50701 cattacgatc aaaatgcctg attcagtacc accagcagta tctaaagcat ttgcagccat
    50761 tttaccagcg acgatcgctt tatacgtcat tgctattatc aattatatcg taggtcgtgt
    50821 agctgatggg caactgatca ttgatttggt gcaaaaatat attgcagaac cattcctagg
    50881 attatctcaa gggttgggag cagtattgat cgtcacagta tttgttcaag tattctggtt
    50941 ctttggtatt catggaccaa atgtattggc accagtgcta gagggaatct ggggacaagc
    51001 acaactaatc aatattgata ttttccaaaa aggttataac ggaaaaactg gtacaccagc
    51061 cgtgttagat gcaatcgaag atggaaaagc ttatatgtgg gtccgcggat cgttcgatgc
    51121 ttttgcatgg tttggcggat caggcggaac gatcgtctta ctgatagcta tcttgatttt
    51181 ctctaaacgt gcagattatc taacagtagc taaattatcc attgggcctg gtatcttcaa
    51241 tatcaacgaa ccagtgatgt ttggtctgcc aatcgtattg aatgcaatca tgtttatccc
    51301 attcttgatt gctccagtag tagcaacagc aattggatgg acagctactt atcttggcct
    51361 agtcgctccg gtatcgcagc aagtcacatg ggtagtaccg ccgttcttat tgtcattctt
    51421 agcaacagga gcggattggc gggcaccgat cgtaacatta gtatgtatgg tcgtgacttt
    51481 cttgatttgg gcaccatttg tcatctctgc aaacaaaatg gaccctgccg caggcgaaca
    51541 acaataagat gaacgtgagg agcgggacgc tttaagtttc gttcctcata atgaattttc
    51601 gaggaggaac tgaaatgatt acaattgcca gtgcaatgca acaagatgaa atcaaagaat
    51661 tgttagaagg atacaatgaa gaaggaatga cttttacatt taaagaaaaa caaggaatca
    51721 agctgttctt tgaaacaagt gcagaagatc cggaagttgc ggctaaaaaa gcaaaggaac
    51781 taatcaaagc acagccttgg ggaacagtcc tttattttca atcaacagct gtttagaaaa
    51841 tggtttaaaa agataggatg gttgagatga taaaaaacgg tttattttta tgtagtattg
    51901 gttctttgat cgtgttgtat agtttgaatc cacattcgca aaggtatgat ttgtatagta
    51961 tggtcacagg tctttttcta acaggattag gaatctattt cttcatgaag gggaaaaaaa
    52021 gagaagaagc aaaggagaaa aataactaat ggaaatcacg aaaaaattaa atattccagc
    52081 atcgtttttt tacgataaag ttatggactc tgtccttttc gatgtacgta aagcgaccgg
    52141 caaatcagta actagaaaac agctgaaaaa tttggagtac gtcaaacaat tctcgcaaaa
    52201 taaccgggct cgcataaaaa tcgaagaagt cattgaaaac cagtcttata agtttcgtac
    52261 atcgacaacg aagaatgaat tcgtcgttca ttatcaaatc aaaccattgg acgattcgac
    52321 ctgtgaggtt cattacacag aaaaaatgga gtcttatggc atgcttcaaa agttgaatga
    52381 tatgctttta ggaacaatct tgatgtattt taaacggaga cgtttcaaaa aaatgctaga
    52441 tatgatcgaa gcaacgtatt aaatcaatga aacatggtac tatttagtta ttgaaaaatg
    52501 aagtaagcta gaaggaattt ttcataggaa gatagatctt tcctgcgaat cctcactagc
    52561 ttttttgtag gaaggggcag caaaatgaca aacaaagtaa gagagctagg tgtatcttcg
    52621 attgaggaaa tgtttgattt agccacttat gcatttaatg gggcagatac acctgaaagg
    52681 cgagaacgtt ttcgtacact tgcggagaat tcttggaatt acggattttt tgatgaggag
    52741 gatcggctaa caagtcaagt catggcgacc ccttttccag tgaattttta cggacaagag
    52801 tacctaatgg ccggcattgg ctatgttgct tcttatccag aagcaagggg acaaggcggg
    52861 atcaatcaga tcatggaaaa gatccttgaa gattgccgtg atcgtaaagt aagcttatct
    52921 tatctggcgc cattctccta tcccttctat cggcgctatg gctatgaaca atcatttgat
    52981 aaaatcagtt atcaactaaa cagtcgtgat gtgccgttta tcaaaaaaaa atctggaaaa
    53041 ataaagcgta tggattttga agatgcaaaa gctgcaatca gaagaattta tgaacagatg
    53101 ccggaaaacc aacgagctgg tctgaagcgc gaagactggt ggtatacgta taaattcaag
    53161 caaaaaggca attaccagtt tgctttgcat tttgatgata atgaggaagc aacgggatat
    53221 atcgtttatc agcttgcaac gtcttccttt attattgcgg agtggggatt tttggatcat
    53281 gatgcttttt gcagccttgt tcgcttcgta agttctcaca atggtgcttt tgaaacattt
    53341 gaatattcat gcggctacgg cggtaatgat caaaattatc tattagaaaa tccatttgct
    53401 tctgtaatga tcacacctta tatgatggcg aggatcgtag atatttcttt gtttttggaa
    53461 aattatccat ttaaaaagag acaagctgca tttgccctac aaatagaaga agatcattat
    53521 gcgaaatgga atgaaggaat cgtggaagta acaatcgaaa atggaaaaaa tcatctctca
    53581 aaagtagaac aaacaaaact cccaatcgtt cgaggaagta tccaacatat cacgcagcta
    53641 ttattagggt atcgtaaagt ggaagagttg gtattccaag aaagaataca ggtgacaaaa
    53701 gaatatgaag aaacgttaag ctcaatcttg ccacagcaac agccaattct taatgactat
    53761 ttttgataaa aaacgggtga tgacaatatt tttgtcacca cccttttgtt tttcatttgg
    53821 catttgaagg aaaatgttaa agtaaatcct cttttttcgt aaatttgaat tttttgtttt
    53881 cttttgtttt agggttttct tttttgtggt cttggattgc ttctttcgta atcttttccc
    53941 gatttttttt agatgaatct aagcgttctg cgtatacttt ttcttttggc ttatcattca
    54001 ttggaaagga cctccctaca ttatttcatt ctacctctag tttatcatct gttaaggact
    54061 agtggaaacg ttttgcctaa cttataagct gattttttta gcagataagc ttgcaagtaa
    54121 aaaacaaaca agctacaatt gatttattga aattgttcct gtagctcagt aggatagagc
    54181 aatcgcctcc taagcgatag gtcgctggtt caattccagt caggaacgta taaataaccc
    54241 ctgtatcctt tgcggtacag gggtttgttt tgtatttgtg ccaaaaatgt gccaaactag
    54301 ttatcatttt tatataaact atcgaacaca gtattgattt tttctttatc ctgatcttca
    54361 agctctcgaa caatatgaag ataagtagac attgtagttt ctaaactgct atgtcctaaa
    54421 cgtttcgata cgctaagtat atttactcct tgataaagta aaatagatgc atgtgtatgc
    54481 cttagtccat gcaaagtaaa ttgcttttca atccctaatt cttttaattt tttgcgcaaa
    54541 tatttgctga cggcgtttga tgaaactaat ccatttttta agttgaaaaa aacaaaatta
    54601 tgagggtttt tgatttcaaa attctcgaat aattcttttt gattgat
//
LOCUS       DACDPS010000010        51194 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.010, whole genome shotgun sequence.
ACCESSION   DACDPS010000010 DACDPS010000000
VERSION     DACDPS010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.010"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            328..1173
                     /locus_tag="IWJ07_000681"
     CDS             328..1173
                     /locus_tag="IWJ07_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9332281.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1454..2674
                     /locus_tag="IWJ07_000682"
     CDS             1454..2674
                     /locus_tag="IWJ07_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAP9332282.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2671..5391
                     /locus_tag="IWJ07_000683"
     CDS             2671..5391
                     /locus_tag="IWJ07_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9332283.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5375..6319
                     /locus_tag="IWJ07_000684"
     CDS             5375..6319
                     /locus_tag="IWJ07_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9332284.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6388..7398)
                     /locus_tag="IWJ07_000685"
     CDS             complement(6388..7398)
                     /locus_tag="IWJ07_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9332285.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7578..7922)
                     /locus_tag="IWJ07_000686"
     CDS             complement(7578..7922)
                     /locus_tag="IWJ07_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP9332286.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7915..8349)
                     /locus_tag="IWJ07_000687"
     CDS             complement(7915..8349)
                     /locus_tag="IWJ07_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAP9332287.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8487..9224
                     /locus_tag="IWJ07_000688"
     CDS             8487..9224
                     /locus_tag="IWJ07_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9332288.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9217..10428
                     /locus_tag="IWJ07_000689"
     CDS             9217..10428
                     /locus_tag="IWJ07_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9332289.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10635..11414
                     /locus_tag="IWJ07_000690"
     CDS             10635..11414
                     /locus_tag="IWJ07_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAP9332290.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11484..12146
                     /gene="trmB"
                     /locus_tag="IWJ07_000691"
     CDS             11484..12146
                     /gene="trmB"
                     /locus_tag="IWJ07_000691"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAP9332291.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12244..13998)
                     /gene="ade"
                     /locus_tag="IWJ07_000692"
     CDS             complement(12244..13998)
                     /gene="ade"
                     /locus_tag="IWJ07_000692"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAP9332292.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14130..15059
                     /gene="cpaF"
                     /locus_tag="IWJ07_000693"
     CDS             14130..15059
                     /gene="cpaF"
                     /locus_tag="IWJ07_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAP9332293.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            15016..16065
                     /locus_tag="IWJ07_000694"
     CDS             15016..16065
                     /locus_tag="IWJ07_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAP9332294.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16062..16340
                     /locus_tag="IWJ07_000695"
     CDS             16062..16340
                     /locus_tag="IWJ07_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAP9332295.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16315..16785
                     /locus_tag="IWJ07_000696"
     CDS             16315..16785
                     /locus_tag="IWJ07_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAP9332296.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16802..17104
                     /locus_tag="IWJ07_000697"
     CDS             16802..17104
                     /locus_tag="IWJ07_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332297.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            17121..17492
                     /locus_tag="IWJ07_000698"
     CDS             17121..17492
                     /locus_tag="IWJ07_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAP9332298.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17464..17823
                     /locus_tag="IWJ07_000699"
     CDS             17464..17823
                     /locus_tag="IWJ07_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332299.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17995..19002
                     /locus_tag="IWJ07_000700"
     CDS             17995..19002
                     /locus_tag="IWJ07_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9332300.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            19027..20211
                     /locus_tag="IWJ07_000701"
     CDS             19027..20211
                     /locus_tag="IWJ07_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAP9332301.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20648..22231)
                     /locus_tag="IWJ07_000702"
     CDS             complement(20648..22231)
                     /locus_tag="IWJ07_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9332302.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22809..22952)
                     /locus_tag="IWJ07_000703"
     CDS             complement(22809..22952)
                     /locus_tag="IWJ07_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAP9332303.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23175..23654)
                     /locus_tag="IWJ07_000704"
     CDS             complement(23175..23654)
                     /locus_tag="IWJ07_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9332304.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23727..24011)
                     /locus_tag="IWJ07_000705"
     CDS             complement(23727..24011)
                     /locus_tag="IWJ07_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332305.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24089..24283)
                     /gene="ssrS"
                     /locus_tag="IWJ07_000706"
     ncRNA           complement(24089..24283)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IWJ07_000706"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24355..25641)
                     /locus_tag="IWJ07_000707"
     CDS             complement(24355..25641)
                     /locus_tag="IWJ07_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAP9332306.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25779..26450
                     /locus_tag="IWJ07_000708"
     CDS             25779..26450
                     /locus_tag="IWJ07_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAP9332307.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26416..26904
                     /locus_tag="IWJ07_000709"
     CDS             26416..26904
                     /locus_tag="IWJ07_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAP9332308.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26917..27864
                     /gene="prmA"
                     /locus_tag="IWJ07_000710"
     CDS             26917..27864
                     /gene="prmA"
                     /locus_tag="IWJ07_000710"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAP9332309.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27865..28629
                     /locus_tag="IWJ07_000711"
     CDS             27865..28629
                     /locus_tag="IWJ07_000711"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAP9332310.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28862..31075
                     /locus_tag="IWJ07_000712"
     CDS             28862..31075
                     /locus_tag="IWJ07_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAP9332311.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31086..31532
                     /locus_tag="IWJ07_000713"
     CDS             31086..31532
                     /locus_tag="IWJ07_000713"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAP9332312.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31703..32962
                     /locus_tag="IWJ07_000714"
     CDS             31703..32962
                     /locus_tag="IWJ07_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAP9332313.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33051..33998)
                     /locus_tag="IWJ07_000715"
     CDS             complement(33051..33998)
                     /locus_tag="IWJ07_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP9332314.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34392..35684
                     /locus_tag="IWJ07_000716"
     CDS             34392..35684
                     /locus_tag="IWJ07_000716"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAP9332315.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35699..37468
                     /gene="aspS"
                     /locus_tag="IWJ07_000717"
     CDS             35699..37468
                     /gene="aspS"
                     /locus_tag="IWJ07_000717"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAP9332316.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37577..38452
                     /locus_tag="IWJ07_000718"
     CDS             37577..38452
                     /locus_tag="IWJ07_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9332317.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38718..40601
                     /locus_tag="IWJ07_000719"
     CDS             38718..40601
                     /locus_tag="IWJ07_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP9332318.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40676..40960)
                     /locus_tag="IWJ07_000720"
     CDS             complement(40676..40960)
                     /locus_tag="IWJ07_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP9332319.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40950..41684)
                     /locus_tag="IWJ07_000721"
     CDS             complement(40950..41684)
                     /locus_tag="IWJ07_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAP9332320.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41813..42436
                     /locus_tag="IWJ07_000722"
     CDS             41813..42436
                     /locus_tag="IWJ07_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAP9332321.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42628..43749
                     /locus_tag="IWJ07_000723"
     CDS             42628..43749
                     /locus_tag="IWJ07_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAP9332322.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43756..46884
                     /locus_tag="IWJ07_000724"
     CDS             43756..46884
                     /locus_tag="IWJ07_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAP9332323.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46903..50097
                     /locus_tag="IWJ07_000725"
     CDS             46903..50097
                     /locus_tag="IWJ07_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP9332324.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50243..50950)
                     /locus_tag="IWJ07_000726"
     CDS             complement(50243..50950)
                     /locus_tag="IWJ07_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP9332325.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16751 a         8946 c        10634 g        14863 t
ORIGIN      
        1 ggcaaaaaga tgaactagaa atagttcatc tttttttgtt taaatctttt gctaagttga
       61 actaatagag tcttttatga atatcgaagt tagtttttca cctaacttcg ttttttatag
      121 ttggttatta taaaatagta atatagctga tttggattca attcatattt atttataaat
      181 caaaaataca gagaatagta tataaatgtc ctttcctatt cttaaaatga tgtttgtact
      241 tacttaaatt aggtataatt agtatatgtt ttgtgagaaa tttagactta caataagcag
      301 tgaatttata atcaacgtat aggagacatg gatgaaatga aactaattgg tatagattta
      361 gacggtactc ttttgaattc tgaccagagc attagtcaaa aaaatgcaag aacaatgaaa
      421 catttaccgg aagactgttt tccattcatt tgttctggaa gagaagtaga agacattaat
      481 aatattctca aaaaatctgg tttaaggata ccagcagtag ggttaaatgg ttctgttgga
      541 tttgatggag aaaaaaagat atttgagtct tccttcgata aaaatgatgt aaaaaaaatt
      601 gattctgtcc tatcgaaatt tccaaccaaa atatatacaa atctcggaag ttatgaatct
      661 cagaattata aaaataaaat gaaaaaaatt tttcacgagg tagggagtga attttctatc
      721 aaagagctaa actatgagct tgagtatgaa aaatctattt gttcaatccc ttataataat
      781 attgaggaaa ttatagaaaa ggatgctatc agcatttata aattttttat ttttattcct
      841 aatcgtcaat tgaaagctaa tatatttaac catttgaaaa agatgctaaa tgtgtctgtt
      901 actgaatcgg cacctgtaaa tattgaaatc gtacctcaaa atgtatctaa aggtgtggtg
      961 tttgatcgtt tggaatcaat ctacaattta aagaaacctt tgagaatcgc gatcggagat
     1021 agtttaaatg acttatctat gtttgaaagt gcagatatta gttttgcaat gggaaatagt
     1081 catcaagaaa tcatgaaaaa agctgattat gtcactgttt caaatgataa agacggagta
     1141 tctcaagcat ttgaaagaat tcttaccata taaactaatt aaggtagtat agaacataga
     1201 attctaaata cggaaaaatg gagcataaat tccagtacaa atagtacttc ttcagtgtca
     1261 aacgaagcga atataacata gatatttagg attgtgacaa aaatcatgtc acaatctttt
     1321 tttcgaataa acttattttt tgaagtcggc catttatcgt aatttctttg cataatacga
     1381 acaaattttc ggttattgat gaaaaaaaac attgaaaatg gtatgataaa agaaaaaaag
     1441 gaggaaattt ttaatggtga aatttatcca tgcggcagat ttacatatgg atcggtcatt
     1501 tgaaggactg acaactcttg ataaaactgt acaggaaaaa ctattgaaga cgaatctgac
     1561 cgttttatca aatatcattg aacaggcaat aataaataac gttgattttg ttttacttgc
     1621 aggagataat tttcatcaaa atcgtccttc attaaaaata cagaaacatt tttcagaaca
     1681 gatgaaacgg ttagaaaaga accatatacc tgtatttata atattcggaa atcatgattt
     1741 ctatcaaaaa gagcggtatt ggttctcctt tcctaaaaat gtccatttat ttacatccga
     1801 aacggtagag acaaaaaaaa taacgataaa aagtggagaa actgtctctc tcagtggatt
     1861 tagttatcgt caaccgtgga ttcaaaaaga caaagtgatg gaatttcctt ctagagaact
     1921 cacagattac catattgggt tgtaccacgg ggaacctggc gtctctcaaa aaggaaacta
     1981 tgctcctttc caaccctcaa agatgcaaga aaagggatac gattattggg ctttagggca
     2041 tatccatgtg ccaacggtct taaatgaaaa gcaaacaatt gtttatccag gtgcgcctca
     2101 aggtcatact aaaaaggaac aagcttctac atctattcta ctagttgaat taagtaaagg
     2161 aagttgccaa atacatccga ttaaagtagc agaggtctat tggaagacaa aggaaatatc
     2221 attgaggaaa gcaagaacga caaaagaggt cattgttcac attcgtcagc agctttcaaa
     2281 agtagaggat ggatttagtt taattgagat aaaattaaaa gattatgacc atctcaatac
     2341 agatgttttg gaacggatac aatcaggaga gctattagac tatttgttag aggaattctc
     2401 agataggata aatgatttct ttatttggcg tatttctctc atagagaata ctttttatac
     2461 gaagacccca cttgctgctt ctgctaaatt gatggaacag ttatttcaat actatcagac
     2521 acctcaagat ttccaacaaa ttttaaatga agtatatagt catcaagaag cggctagaat
     2581 attgagcgaa ctgccggaat atcaagaaga aacacttgaa aaagccaagc agctgcttaa
     2641 tcaagatttt ttatttgagg aggatcagga atgaagattt taaggatcga gattgctgct
     2701 tttggaaaat ggcgtcaaaa aagttttgac ttttattcag gcaatcagct gatctatggg
     2761 ggaaacgaag caggaaaatc aacgatttat caatttattc aagcaatatt atttggtttc
     2821 ccttcaaaag gccgtaaaaa aaaggattat acaccaaaag acggctcagc ctatggcggc
     2881 aaaatatggc ttaaacatcc tgtatatggt gaatttgctg tcgagcgcta caagcagcaa
     2941 aatcgcggaa aatcgaaggt gtggctgggg gatcaagtgg gaagtgatga gttactagaa
     3001 aaactgatcg cacctttgac gaaagagtta ttccagcagg tatttacttt ccagcaagag
     3061 cagttgatgg aactagatca tttgagagaa aaagagctac atgatgcgct gatttcttta
     3121 ggcatcacgg gcagttctca agtattccaa aagcggcaag aatattatca gaaagcacaa
     3181 caattattca agaaaaaagg ccaaaaatta ctaatcaatc aaaaattaaa tgaatggaaa
     3241 gagctgcaag aaaaaattca tcaaaaacaa gaacaggaag cttattttgg caagttgatc
     3301 caacaagaac aggaatacga gtcgcaacag cagcagttat tggcacaatt aaaagaggcg
     3361 aacaaacaat tttcattagc cagacagcaa gaacttaatt tttctttata tgaagaatgg
     3421 caatctctca aaaaaaacag cactacaggt cttcctgatg agcaacttct tttagatctg
     3481 gagtctttct ccaaacggta tcagcaaaca aatgagcgtt tggaagaatt agaagcaaca
     3541 ctggaaaagc atagcgggat ggatcagcag tcagccagat attattttta tttagagcag
     3601 gagcaggaac taaaagatct ccagcaactt caatttacag cggaacaatt aaacgaagaa
     3661 tggcaaagat tacaaattga gactagagaa acaaattcag aattgaagat tttggaacaa
     3721 aggtggcatt ggcagaaaaa tccgttaccg gaagaatttt tatatgaaga agaatggaag
     3781 catcttctgg ctgaagaaag aaggattgct gatctagaaa gccagatttc tgtgcgtctc
     3841 caaatagcta aagaacagca agagatgatt gaagcagaag tgaatgactt ggaagagacc
     3901 tatccagcat tattacaaaa tcagtcagag atagcaccag tctcttcatc taaaccgtat
     3961 atgctgatgg ctggcggagc aataatcatt gcaggtatat ttgtaccaat gcccttgaaa
     4021 gtgatccttt tactaattgg tgcaggcatg attggctatt tcttctacaa aaggcaacca
     4081 aaaattaaaa ctaaaaatga tttctctgag ataaaaggaa tgtggcaaga aaaactagga
     4141 caacttgatc tagccttagc tcaattagct gaagcagaag aaaaacaaaa gagaactgga
     4201 gaagaaaaac agattcttca gcaagaagta tatcgttttg cggatcatcg gcacttagga
     4261 aagatggata cgctcaaact catgaaagaa tatggacagg aagtcataga ttatcagaaa
     4321 ttatatggcg tatttgagcg gaaacataaa agagaacagg aaatacaaga aaaactacga
     4381 caaatcgacc aaaaatttga gtttgttaaa gaatggttac cgatacaaga taagacaatt
     4441 tcagaaaaaa tggtattgct ttcccgtttt atacaagaga tggaacagat gaaatttgcc
     4501 agaagctatc agcaaaatac tttattgaag caagaaatca atcaattgaa aaagaaacaa
     4561 aaagtatgga tacaagaata cagcgaacaa ttgactcaag caggcttatc ttatccttcg
     4621 gaagtacctg tctttttaca aaaagccaaa caagccgaac agcagatgaa aagatacaat
     4681 gaattatcgg aaatgcttac tccactattt cctgaagagc tgacaaaaga gaaattatct
     4741 gtaagactgc gccaactgaa agaaaagcaa tcccaactcc agcaagaaga aaaagaagca
     4801 agtgaaaatc ggcaacgact ccaacttcaa gtagaagagt tgcaaagaga cggcacattg
     4861 gacgaacttt atcaacaagc tagccggcaa aaagcaaaat tacaggaatt actggaggaa
     4921 tggggagcac ttgaaatcgc cgggacattc ttaggtgatc tttcaacaga gatgagtgaa
     4981 agacaattac cccagttgtt aagagcagca ggtgattatt taggtatttt aacaaaagga
     5041 aattatcaga agatattact gaaaaatgat acactgactt taacagacgg atatacagaa
     5101 tttcccatct atgagttatc tacaggtacg aaagatcagc tgattatggc gatgagattt
     5161 gcttacctct cattagaagc taaccgttcg atatgcccga tcatcattga tgatggctgg
     5221 cttcattatg atcatcagcg aaagtatcaa ttagctgaac ttttagctaa atttggagaa
     5281 aagtatcaaa taatttgttt ttcttctgat aaagaaatgg taagctatta ccatgagatg
     5341 aaccaaatcg taaaacaatt agaaggagca aataatgaaa aaaattcgtg atttagtaat
     5401 cgatgagcag tttgaacaat ttgtcctgat caaaagtgca gatgtaagaa tcgctaaaaa
     5461 cggaaaaaaa tttattgctt ttactttcca agatacttca ggtactatcg atggtaaata
     5521 ctgggatgct tcggaggagg aaatcaataa atttacagcc ggcacagtcg tttttctaaa
     5581 cggtaaaaga gaggtttatc aaggaaaccc tcaagtaaag attcttcata tgcgcttaac
     5641 aagggtagat gagccaggag agcctagttt atacatggaa agagcccctt tgaagaaaga
     5701 agaaatggaa gaagaaatca atcaattgtt gtttgagatc accaatgcgc attggaatcg
     5761 tattgttcgc tatctactta ataaatatca aaaacaattt tttgagtttc cggcagcaaa
     5821 aagaaaccat catgcatttg ccagtggatt agcttatcat actttgacaa tgcttcactt
     5881 agggaaatca atcgccgatg aatatcctga gctgaatcga gcattgttgt atgctggtat
     5941 cattttgcat gatttaggaa aggtgattga actttccggt cctatgtcga cagaatatac
     6001 gcttgcagga aatttgatcg gtcatctggt attgattgac gaagaaataa ctaaagcctg
     6061 tcttgcgttg aaaatcagcg aagctgacga agatgttctc gtattacgtc atatggtttt
     6121 agcacaccat ggcttattag aatatggctc acctgtacgt ccaagaataa tggaagcaga
     6181 aatattacat caaatcgaca atatcgatgc ttcgatgcag atgatgttga cttcgattcg
     6241 tcaaacagaa ccaggaaaat acaccgatcg gattttcggt atggacaacc gaagtttcta
     6301 tgtaccaaaa gatttatagc aaaaatttat ttttttcatt caaaacaaaa aaagcgagac
     6361 ataagtcttc gctttttttg ttttctttta ttttgatgaa tcagttgttt cttcagttga
     6421 agattctttt gttgaatcag ttgattttgt gtcagaactc tttgttgaag ctgtagtttc
     6481 acttgagtct ttcgtacttg atgaagttgt tgtgaatgca ctcaacacat tttcaaaggc
     6541 atcatctttg atttttacat ttgcatcttt taattcttca ccgattactt tagttgtgaa
     6601 tgcattgtca cttagtttag tttcagtagc gatatctttt agttgatctt tgtatttatc
     6661 catgtcattg cctttatttt ggtttttcac cattttgaca acgtagtatt ctgtcgcata
     6721 agaagtagga tttgttgtag taataacatc ggagatctca ccgtctttca atttgaaagc
     6781 tgcttctttt acttctgcag gaatagttgt tgtcgttgag tcgaatttaa ctttaccgcc
     6841 atcttctttt gtctctgtat cagttgattt gtctttggct aatttagaga aatcatctcc
     6901 atcatccgct gattttttca catcttttgc ttcatcttca cttgataatt tgatgatttg
     6961 tgcttctact tctggatgga aagatttcca agcagttttc aaatcgtcat ctgtaatatc
     7021 tacgtgagct ttcaagccag cttcaaaagc taaattgtta cgaatatatt ctttataagt
     7081 atctttcgta tatccagcag cttctaattg tgattcaaaa gtatcaccta atgatttcgc
     7141 ttgtttgtcg tattctgcat ccacttgctt atcggttact ttatcgccat atttgttatt
     7201 gaatacttta taaatgatca tccgttgtac taaggattgg ttcgatgatt caagttttgc
     7261 ttcatcatag aaatcagata cagtgatcgt tccgcctttc atcgttgcga tatctttatt
     7321 agtatcgcct gaacaagctg ctagagttaa aacagccaac gcactagtag ctgccaaaat
     7381 aattgatttt tttttcatta aaatttgcac tcctgtaatt agttattatt tttatatatt
     7441 aactatacca tatcccattt cctttaaaaa cgttgtcgtg gtagtttcaa acaaaattca
     7501 aaatttcatc acattcctag gaaatacgca aacttttctc acttatatag ttaaatctgt
     7561 tgagaaaata atcattatta tatttgttca ggatactctg gcagatcagc ttttagtttt
     7621 tctacttgtt cattgatttg tgtgatacgt ggttcggctt ggaatttaaa atcttcgata
     7681 tcttttttga ttgcttcttg gaaagcagga atcacagatt cagctgtttc ttttgtttcg
     7741 atcaatgcgc ttttaaaatg attcaggctg ttatttaaat catttgtcag agttgttgct
     7801 tcttccaatt cttcgatcag tttctttctt gtttcattcc cgctcctggg cgccaataac
     7861 agacctccga cagtgccagc agcagctcca aataacagtc ctttgaaaaa gtttttaatc
     7921 atcgcaggac acctgcttta cgattgtttc ttgaatagct tttagttctt ccgatccata
     7981 actatcttga tgattgacaa agtgaataga aaaatcatcc tctttgctgt agcgagggac
     8041 taaatggata tgtgaatgaa aaacagattg ataggctagt tcacggttgt tattcaagat
     8101 attaagacct ttcatttctg gaaaagcttt ttccaatgct tgagcaatct taggcacacg
     8161 cgaaaaaacc tctcctgcaa gttcgggatc atattcaaaa atatctgcta catgacgttt
     8221 aggcacaact aatgtgtgtc ctttcgttgc ttgagagatg tctaaaaaag catagacctt
     8281 gtcgtcttca tatattttat agcttgggat ttcttgattg atgattttgc aaaaaataca
     8341 gttttccata taaaacatcc tttctataaa gaataaatac ttcagcttaa ctttaccata
     8401 agttaaagct attgtaagga aaaaaacaaa ataccgtcat tttcttcgtg gtataataaa
     8461 taaaaataaa tggcggagga aaaagaatga gtttaattat cgatcatgtc acaggggggt
     8521 acggacatct ccctgtgtta aaagatatca gtttcgatgt acagcctgga gagatagtag
     8581 ggttgattgg actgaacgga gcagggaaaa gtacgaccat caagcacatt atcggtctgc
     8641 tggaagcttc aaaaggaacg atagaaatca atcatctatc cttaaagaaa aatacggaag
     8701 aatatcgcaa acaaattggg tatattccag agactccttc tttatatgaa gaattgacat
     8761 tgagagaaca tatcgaagtc actgcgatgg cctacgatat acctatggat atagcaatga
     8821 aaagagcaga aaagttgttg gagacttttc ggttatctaa tcgtcttgac tggttcccag
     8881 ctaatttttc aaagggaatg aaacagaaag tgatgatctt atgcgccttt ttgatcgagc
     8941 cttctttata tatcatcgac gaaccatttt taggcttgga tccactagct atcaatgcat
     9001 tgcttgaatt gatggtagaa atgaaaaaag aaggagccgg tattttgatg tctacgcata
     9061 ttttagctac tgcagaaaaa tattgtgatc gttttgtggt cttacataac ggagagatac
     9121 gagcagacgg ttctcttgag atgttgagaa aagaatttca gctgccggat tcttctttgg
     9181 acgaaatcta tattgcttta acaaaggagc aggcacatga gtagtttttt tcaaaaacgt
     9241 ttagcaagac atcaaaagaa aatgatgaaa tatatgcgtt atgtattgaa cgatcatttt
     9301 gttttagttt gtcttttctt gattggcggt gtgggattat attattctaa ttggttgaaa
     9361 accctgcagc caccgtttgc aattggaggg ctcataatct ctgtattttg gatgctctgt
     9421 ttattcgtgg gtaagatggc tacttttgca gaggcagcag atattgtttt tttacttcct
     9481 aaagaaaaag aaatgcaaac ctatttggaa cgagcttttc gctattcttg catttttccg
     9541 ttgatctttt tgatattggc atgcggattg gccatgccat tagtggtggt tagtacggag
     9601 caatcctttt cgatgttttt cgtctatctg atcatattat ggagtttgaa attcagtcat
     9661 ctgcaaatca agagagcaga attgtttcga ctagatcaac aagttattcg ttattggaaa
     9721 ttaggctggt ttttttcttc tctgatcttt ttgtttctat cattttattg gctaatatgg
     9781 gcaggaatag caggtgcatt agtacaggca agtctatatt actggttttt gtggaagcaa
     9841 caagaaaaag gattggattg ggagaaaatg gtgcatgtag aagaacatag gttgcatcag
     9901 atctatcaat ttatcaattt attcacagat gttcctgaaa ttacggcaaa gatcaaaaga
     9961 agacgctatt tggatcctat attgactcat attaaaagag aatcaaaaaa cacttatctt
    10021 tacttgtttg caagaagatt ggcaagaggt tctgatttta gtggtcttta tttccgatta
    10081 gtaattatag gtgggttatt gattgccgga gtagtcgact tttattttag tctgattatc
    10141 ggtttcttat ttttatattt gatcggtttc caactgttac ctttagcgaa ccagttccgc
    10201 tatatggttc tcgttcagct ttatccaatc cataagaatc agaagaagca ggcgattcag
    10261 tatctactca catgggtatt aattatcgcg tcggttattt ttggtttgat tgctgcagtc
    10321 gtattagatg ggcagcagcg atgggttccg ctagcggctt atttgattgt tactatgctc
    10381 tttgttaaac tatatgtacc aactcgtttg aagaagatgt cagactgaaa acaaagctgt
    10441 gtagggtaaa aatgagaatg aaaaatagag cgttgacagt aagaatgctg ttcgttctat
    10501 tttttttgag aaaagctggt tttttgaatg gcaattaaat gaaaaagaac gtatatgtgc
    10561 ttgacgtgta gtgttcaggc gactagaata ataagtactg caaaacaaag agtaagagga
    10621 cgggagaatg catgatggag tttcagttag accaagagtg gcggttacgt cccatcaaag
    10681 gcgacacagg aaaaacatac ataggcatgc gtgatgagga caaagtcttt attaaacgaa
    10741 acacgactcc gatgttggct gctctttcaa gggaaggaat tacaccaaaa cttgtttgga
    10801 caaaacgaac ggggaatggc gatactttga cagcacaaga gtggctggaa ggtcgcttgc
    10861 tgactcctaa tgagattggt caaagaaacg acgtgattga tgttttgtac catttacatc
    10921 attcaaattc tcttaaaacc atgctgaccc gtatcggtgg tcgtgtcatg acgccggaga
    10981 agatgctgca aaaatatgaa aaagaattac cggaagaact ccagaaaaat caatttctgc
    11041 aaattgtcca tcgttttctt cgtgagcaga tcccgtcaat agataaggaa aacttatcag
    11101 tagtacacgg agatgtaaac catcgtaatt ggttagtatg taaaaattat ttatatctag
    11161 tcgattggga ttcaatcatg tttgcagacc ctgcggtgga tattgcaacg attttaggaa
    11221 attatgtgcc actgtctagc tggaatcagt ggctgatcag ttatggcata cgcccgactg
    11281 acgaaatgct tgaaaaattt cattggtatg cggccatgaa tttgctccaa gaaatcacca
    11341 gatattgcaa acgtgaagat catcgacgga tgaacgaaga aattttacaa ttaaaacgaa
    11401 ttttcagtgg ctgaggtttg gcccaaactc tgcggagatt tggccagacc tttttaatta
    11461 gaggaaagga atggagataa ataatgcgtg tccgaaatcg tccgggagca gcggaaatgt
    11521 tagcggctca cccgaatttt gtaattagtg atccaacatt gtggaaggga aaatggaatg
    11581 aattgtttga aaacgatcat ccaatccaca ttgagattgg tatggggaaa ggccagttca
    11641 tcactggaat ggcaaaagcc catcctgaaa tcaattatat tggagtagaa atgcaggtca
    11701 gtgttgtttc gattgcacta gacaagttga tcgaacaacc gcttcctaac ctgaaacttc
    11761 tacatgtgga tggatcagcg ttgacagagt atttcgctga cagcgaagta gatcaaatct
    11821 atctgaattt ttccgatcca tggccaaaaa aacgtcatga aaaacggcga ttgacatata
    11881 agacttttct tgctgtagat gaacagattc ttcgtccaaa tggagaaatc catttcaaaa
    11941 cagataatca aggattgttt gagtattctc tggcaagttt ttctcaatat ggcatgatcc
    12001 tcaaacaggt atggttagat ttacatcaaa gtcaatttga aggcaatatt atgacggagt
    12061 atgaagaaaa attttcttca aaaggtcaac gtatttatcg agtagaagct caatttcaag
    12121 ataagaccaa aaagaaaagt ctgtgaaact aactttctgc tttcataaaa acattaatat
    12181 ccataaataa atcccagaaa atgaacatta cagatgtaat gtattttctg ggatttattt
    12241 gatttaaaat gtatttattt catcggtttt tccacaaggt ggaatcaatt ggaagcgaga
    12301 aaatgaaaat aatcctttgt cggtgatctt caattcgggt atgacagata atgttaagaa
    12361 agaaagtgtc aaaaaaggat caaaggtttt agctgctcct agttgatgtg ctttagttat
    12421 taattcattc aactgttcat agacgatcga aaaaggttcc tgtgtcatga ttccgcctat
    12481 aggaagagaa acacaagcta tttcctgctg tttgttgaca acggtcatcc cgccaccttt
    12541 ttgaatcaaa agattagcag catacagcat atcctcgtcg tttgtcccaa cgacaactag
    12601 attatgcgag tcatgggcaa tcgttgtcgc tatcgctcct tgtttcagct gaaatccttt
    12661 cactatgcct aatccaatgt ttcccgtttg atggtgacgc tcgatcaccg caatctttaa
    12721 ttggtctttt tcgattgaag gaatgaatcg ttcgccttgt cggtccactt tttctaccaa
    12781 atctttagtc actagactat tcgggataat ttcgatcaca tgggcttgtg aagaagaaag
    12841 aagcagctct aacgaatgtg gatttaatgg ttgagcgttt agttttggca gacaacacgt
    12901 atatgaattt gaatgatcag caaataaatg gtcttggagt tgcccagatt gaacaactaa
    12961 tgttccctta tagaaaactt catgaattgg cagcttggta agatcgtctg ttaagaagaa
    13021 atcagcttga tacccagggg caatcgcacc tagtgttttc aaatcaaaac attctgctgc
    13081 attcaatgtt gccatctgaa tggcttgtaa tggatcgatt ccaagtgcaa tagcttgacg
    13141 gacgatatga tcgattgatc cttcttttac taaatcattg atcagtttat catcggtgac
    13201 aaagaagcat cttcttgaat tttcaggtgt gatcgccggt agtagcgatg ccagatcttt
    13261 tgcaacggtc ccttctcgaa ccattaaata cataccaaga tccaaacgtt ctttagcttc
    13321 ttttgcagtt gtagcttcat gatccgtccg aatacctgct gcaggataaa catttaactc
    13381 ttccatgccg ataccagctg catgcccgtc aatttttttc ttatgctggt gcatcaattg
    13441 gatcttttct aggatgtctg tttcattatt tgctactgct tgatagttca tgacttcagc
    13501 taagcctaaa acttcgggtt tttgtaaaaa aggggctaat ttttttgcat ttaaatttgc
    13561 accattggtc tcaaaagggg ttacaggtac acaagaaggt agcataaaaa agatattcat
    13621 gagtgtttgt cttgcatctt ctatcataaa ctcaagacct gtactcccag caatgtttcc
    13681 tatttcatgg ggatctgtca ctacagtagt aactccatga agcaaggata cttttgaaaa
    13741 ttcgcttggt gataaaagtg agctttcgat atgcacatgg ccatcgatga atcctggcac
    13801 tagatattgc ccattagcat tatgtacgct ttttccttcg tattcaccga ttcctacgat
    13861 cattccttcg cagattgcta catctttttc catgatttct ttcgtaaaga cgttgacgat
    13921 cttagcatgc ttgatcacta ggtcagcagg ctttttttta cttgctactt cgatatattt
    13981 ccttaatgtt gtttccaatg gagtgcctcc caagataaat ttttataagt ataacataaa
    14041 ttcggttttt gccaatattt ttttgtaaat tcattttatt tgaaacaaaa agatcaaaaa
    14101 gacgtatgtt ttttgtggag gggagaataa tggaaataaa acgattatcg gatcaaatga
    14161 taagtgaaag ttttgccaaa catgcccaag acgtatatat attgccagct tcagaagtat
    14221 tttacatcta ttttcgctat ggaaaaaaac gaacattctg gaagaaagtc tcttcggcag
    14281 atggacagca actgatctca agatttaagt atttgggaca gatggatatc ggagaaaaaa
    14341 gaaaagctca gttaggtgca tttacttatt ctttagaaaa acaggaagtt cgtctgcggt
    14401 tgtcttctgt aggagattac cttcaacgag agagtttagt gattcggcta ttatacggta
    14461 cttttgaaac accccatcgt tgctttagtt ctcgagattt tgcaactatt ttacagaaaa
    14521 ccgtcagacg tggattatac cttttcagcg gaccagtcgg atcaggcaag acttctctaa
    14581 tgtatcggtt ggctttggaa gaaaaattgc aagtgattac tatcgaagat cctgtagaga
    14641 tagaagaacc gttttttctg cagctacaga tcaatccgaa aatagcacag acttatgata
    14701 cacttttgag actcgcatta cggcatcgtc cagacttgct aatcattggc gagataagag
    14761 atgaatcaac tgcacatgct gcggtaagag cagcactaac agggcaccgg gtttttgcaa
    14821 ccgtacatgc acgcaatctt gaaggtacat tggtcagaat ccatgaactg acaaagaaaa
    14881 aagaagagct atctgaatgt ttagctggtg tcatttatca agaacttttg gaagatgcca
    14941 atcaccatcc ttctgtttta tggagttatc aatttatgga ggaagaatca gtaaaaaaat
    15001 catgggagga aagttatgaa gaagctcagg aaaatatttg gcagtcagaa ctattctaaa
    15061 ggattaacaa gaagccagca aaaccagttt gcacaaacca tgggagaact gcttttatct
    15121 ggttttagtc tgcaagaatc cctttattta ttactcacga tccgttcgtt tccacctgtt
    15181 attttggcaa atatccaaca tcagctaaag gaagggcaag cgtttagtga ggtactttcg
    15241 caaataggat atggagcaga gcaactggta cagatcgagt tagctgaacg tcatgggaat
    15301 ctgccgacaa ctttgttgga aattgccaaa cagtttcggc ttgttgaagg atttcgcaat
    15361 gatttgaaaa agctgcttgc atatccgatg ctattgctca cctttttgct aggtatcttg
    15421 tttagtatgc ggttgttcgt tttgcctcag cttattggtt caggaatggt catgcaagag
    15481 cattggggta ttcagctgat acaagtcttt cattggtatg ttctactatt catcctagcg
    15541 gttactctat tggctttact agtaaggtat cgtctgatga agttgtctcc tgttgatcag
    15601 agtgaatgga taagcagaca attattgatt ggaccgatat attccttgta tcaatcggct
    15661 tatttgtcac tagagattgg aaagcttttt catgaaggtt tcgaattaag gcaaatcata
    15721 gtctgcttaa aggaaacgaa acaagaaagt ctgattcagc atcttgcctc taagttgatt
    15781 caaggattgg aggcaggtat gtcattggct gagcagttcc agaattatcg ttttctatca
    15841 gaagaattca gtaaaattgt tttacaggga gaggcaaagg gaagtttagg taaagaactg
    15901 attttttaca gcgaatggac aagaaaacag cttttcaaac gtcttcatca ctggatgcat
    15961 tttattcagc caatcatttt tttgggcgta gcggctttga ttttattagt ttatgccgct
    16021 gttttattac ctgtttacgg aagcatagag gaggttttgc catgaaaaag agaaaaatat
    16081 cttattatag cggttttaca ttgatcgaaa tgctaatcgt cttactgatc atcagtgtat
    16141 tagtgttgtt gttcgtccct aatttgtcgc gttataggaa tcatgtagac caagaaagtc
    16201 gtgaagcgat catccagttg gtagacacac aaaaagagct ctatgcgctt caaaataatg
    16261 gaagagttcc tactgtggag gaattgttga atgaaggcta tatcaaacga gaacatgcag
    16321 aaatctatca gcggccttga aggtaaggtt ttagcatcag gatatacgtt gtttgaatca
    16381 ctcttactga ttttattact tacaacgatc ctttcttttc cagttctcgc attttctgca
    16441 tggaagaatg aattggcagt ctatcaattt ttcggccaat ttgaaaaacg aatctattct
    16501 acgcaaaaaa tcgctattgt gaatcagatc caaacaggct tttattggaa tagtgaagag
    16561 aatcaaatca tttttcatgt tccgaatcca gataaaataa attgggaagt attagatatt
    16621 cctgatgaga ttaccttgaa acgccatgct tctattactt ttgctgctag aaccgggaat
    16681 gaaagttcac taaaagctta tcaattttat tgggaagaaa aagagcggac gattacgtat
    16741 caattccaga tagggagtgg tcgctataca aaaagaattg aataaaacag gatttgtttt
    16801 gttggagtcc cttacagcgc ttatgatcgc ttctgtctgt gtctacttag tgagctttgg
    16861 ccagctgttt ttgcttcagg cagaaaagaa aagtggggag gaactgctat ttattcgaat
    16921 gatttatgaa gatatcaaga cgcagcggat gtatcacaaa agaccagagc gaagggaaga
    16981 aatgatccca tacgatatcc aaattgtcga taataatgga gtaatcgaaa aagtgtttgt
    17041 caatatggag aaagaagcga tcagtattga aaaaacaagt aattccagca tttacgctga
    17101 ttgaatgtct aattggactg atgatattga gcagtctatt gctcacattc aatcttttga
    17161 ttcagcaaac catccaagta agtgaatttt tgcagcgtaa agatcaaaaa gagtggctaa
    17221 tatttttagc acaattagaa gaagaactca agagcagtca caatgtctct gtgaaaaatc
    17281 agcaactgca ctacacgatc gaggataaac agtatatcat cgaaagatat caggcaatga
    17341 tacgtaaaag aagaacttcc ggcggacatc aacctatgtt aacaagtatc actgagcttc
    17401 aattattgga aaaagaacac acaatttctt tttatgtaca ctttgaaaat ggagaagatg
    17461 gatatgccat atggacaaaa aacgatcagt agaaaatgtt ataagggctc tattttattt
    17521 tctgtcttgc ttttatttct tttatgtacg tttttattga tggcagcatt agaaagctac
    17581 cgtttgtcaa tggatcttaa tatacgaatc aaaaattatt atatagcaaa aataatgaag
    17641 cagatggctt tatcggaaca gtctacgctt gccgaaagtt cggaaggagt tttttactac
    17701 acaactggat ctgtaacata tcagtgggta caacagtccc tattcttaga ggtggaagtc
    17761 tcaccgttta tctttcggtt cacagaagaa gtaaaaaatg atacagatac tagtacagaa
    17821 tgatggaatg ttcgtgaaaa cgccctcttt tctgttataa cactgttttt aataaaaaaa
    17881 tgtttgccgt ttcacaaata aaaaacagtg aaaacgattg aaattttgtc aattgttcga
    17941 tagaattaaa caggtgaaaa aattgttata atatatctag aaatgaggtg caccatgtta
    18001 ccagaaaaaa tggaacaagg gtttaatcaa aacctggaag ctattcagct tttacagaat
    18061 gcattgggaa cttccttttt agaagcctat gtcgaaaatg cagaaaattt aatagatgat
    18121 tatcaagtac gtgtggtgga tggagtgccg acaaaagaga caacgcaacg gatcacagct
    18181 ctttatgaag agttgaagaa actttctttc gagccagaag aatggcgcag actgtctcaa
    18241 ttacttttat tgaagggcag ccagacagaa catttgcagg cgaaccatca actcactcct
    18301 gatagtatcg gttttttatt tgtttttctt attgaacaac tatacacaaa taaaaaagca
    18361 ccggttaaga ttctggatat tgcggcagga atgggcaact tgttattaac agttctttta
    18421 aacctatcga atgcggggta ccaaacagaa ggaattggtg tggatattga tgatacattg
    18481 ttggctgtcg cagcttctac aagtgatttg acacaagcaa atgttcaata ctttcatcaa
    18541 gacgggttgc aggaattatt aatagatcct gtcgattttg caatcagcga tttgccaatc
    18601 ggttattatc cgaatgacga aaaagcaaaa gagtttttaa caagtaccga agaaggccat
    18661 agttatgcac atcatttatt gctagagcaa tcaatgaaat atgtgaagcc ggatggtttt
    18721 ggtttgtttt tgatgccgag tggattttta gaaacggatc aaagtgaaga aatcaaaaaa
    18781 tggtttaaag aagaaggtta tcttcaagga atgattcaat tgccagatga attgttccgt
    18841 aataaacaat cgcaaaaaag tattttgatc ttacagaaaa aaggaccaca agcacaacaa
    18901 gtaaaagaag tcttgcttgt gaaacttgct tcattaaaag aaccagagaa ggtgacggaa
    18961 tttttcaacg agtttaaaaa ctggaaatca tctgctctgt aatcattcaa cgagaaggag
    19021 agtcatatgt ctaaaacaat tgcaatcaat gctgggagtt caagtttgaa atggcaatta
    19081 tatcaaatgc cagaagaaac cgtagttgcg aaagggatcg tcgaacgtat cggtttacaa
    19141 gattctattt ttaccatcaa gtacggcgat aatcaaaaat tcgaacaaat catggatatc
    19201 gaaaaccacg aagtagcagt aaaaatgctg ttagatcagt tgattgaact gcaaatttta
    19261 agctcttatg atgaaatcac cggagtaggg caccgtgttg ttcacggagg agaaacttat
    19321 ggggattcag tagtgatcga tgagaaagta atggaccgca ttgctgaatt agcagaattt
    19381 gctcctttgc ataatcctgc aaacttaatg gggatcaaag cgtttaaaaa gattttacct
    19441 gacattttga gtgtggctgt attcgataca tctttccact caacaatgcc aaagcaaaat
    19501 tatttatata gcttaccggt gtcttattat gaagatttca aagtaagaaa atatggcttc
    19561 catggcacaa gtcatcgtta tgtgtcggaa cgtgcggcaa aattattgaa caaaccgatc
    19621 gaagaactaa aaatcatcac ttgccactta ggaaatggtg tatcgatcac agcagtcgat
    19681 ggaggtaaat ccatggatac ttctatggga ttcacaccat tagctggggt gacaatgggt
    19741 acacgttcag gtgatatcga tccagctgtt ctcccatatt taatggaaaa attagagatt
    19801 gatattgatg aaatgatcaa tgttttgaac aagaaatctg gtctgctagg cctaacagga
    19861 ttgtctagtg atatgcgtga tcttgaaaag aactatgaaa aagaacatat tcgtttagct
    19921 tatgacattt tcgtggaccg catccgaaaa tacatcggcg gttatgtgac aacgatgaat
    19981 ggtgttgatg caattgtctt tactgctggt atcggcgaga atgacggaca tgtacgtagt
    20041 gaaatcatca aaggtatgtc ttggtttggt tgtgaaatcg atccagcgtt gaatgaattg
    20101 cgtggtgaag aagtagatat ctctacaaaa gaatcaaaag taagagtatt agtgatccca
    20161 acagatgagg aactaatgat tgcccgtgat gtggagcgtc tgcgcggcta agccaaagca
    20221 ttcgttagga taaaagatga aacgcatgca agagggaatt cttgcatgtg ttttttttgc
    20281 ggagaagacc gcaaaatggt aatttaactt caatttccct tcttttttac aggatcttct
    20341 tagggaattt tgcttggaaa agtggtcaaa agggaaaaga ctggcatttt atttattgat
    20401 tttcaggaaa atcccaatat tttattttca ttcatcttta taagggacat tacatatgta
    20461 aaaagaaaaa acatttttag atcagcctcg tgggctgtcg actttattct tcgctttatg
    20521 tttagctaga aaaccgatcc gaaacattca ttgatcgaat aataaaaact tgattttcca
    20581 cctgtacttt gcctgaatcg tgaaagcaca ggtatgaaaa tcaagttttt tgttgcgaaa
    20641 aaacgtccta gtcaatcagt tggccgagcg gctggcggct gatctgctcc tgatcaataa
    20701 cgagtgcttc ttggaagtgt tgctgcaaaa agtagcggtc atggctgact gctaggagcg
    20761 ttccttgata gtcttgcagc atttcttcaa tttcttctcg agcatagctg tcgaggtgat
    20821 tggttggttc atccaaaatc aggacgttga ttttttctaa aaagagtttc agcaacgcca
    20881 aacggatttt ttcaccaccg gataaatctt tgatccgttt acccacatcg ctttgataaa
    20941 agccactttt ggctaattgc cgtcgagcga cttcttcgtt tccagtcaat gtgatggcgt
    21001 aagccagcaa acggctatcg ggatcggcaa actgcagctg ctgcggcaaa taaccgattt
    21061 gcaagctact acctaatttg atggtgccac cgtaaggctt ttcctcaccg agtaataatt
    21121 tgagtaaggt tgattttcct gcaccattgt cgccgatgat ggctaaatga tctccacgat
    21181 agactgcaaa actgctgtct ttgaatagta cttgatcagc gtactgctgg tgcaagtttt
    21241 gaatgatgaa aacttctttg ccagattttc caccattaga caagctttgc agccgatttt
    21301 tcgggggagg tggcggtttg acgagtgtca gtttcgccag gcggcgttcc agttctttgg
    21361 cttttttgaa gaaggactca ttgtctcctt catgtgccca ctgacgaaaa cgacgaatca
    21421 tgactttcaa ccgctggact tctttttgct gcagttcgta attttttgtc agctcagcca
    21481 agcgagcttc tttcaagact cgaaaacggc tgtagtttcc gggatactca atcaatgcac
    21541 cttcttccag ttcccatgtt tttgtggtga cttgatcgag aaattgccga tcatgagaaa
    21601 cgaccagaac cgattttttt gtattctgta aatagttttc caaccagcga atactgttaa
    21661 gatctaaatg attggtcggt tcatcgagta aaagtaaatc ggcatcggct gctaaaattt
    21721 tggctaaagc gactttaact cgttcgcctc cgctaagttc tgttaaccgt gcttcctgac
    21781 ggtgtggaat ccctaagcct tgcaagatcg ctgcaatccg gtcttctaat acgtaaccgc
    21841 ctagttcttc aaagcgatgc tgcagctcac cgtaacgggc cagacacttc tctaagtttg
    21901 tatctggttc tgtcatcaat gcttcgtagc gactcatttg ctgtttgagg tcctgtatct
    21961 cagcggcaga atggagcaag tagtggttga cggtcgcttc ggaaactgta agttcttgtt
    22021 cgaccgtccc gatctttaag ccttttttgc gactgataac accgctgtcg acaccttctc
    22081 tgccggtcaa gatttgtaaa aaggttgatt tgccagttcc gttgataccg actaaggcaa
    22141 ttttttcacc agcttcaatt tgacaggata aatcctcaaa taatggattc ccgccgattg
    22201 tttttttaat agaaattgct tgtatgatca tgatctctct ccttgagacc actcaaaaag
    22261 gccatggtta tccatggcct gtgttattta aactcagatt atcatgagaa aaataccttg
    22321 agatggccat tgattttacg tgaattcaac tgttttggac aaacttgtcc cgtcaacagc
    22381 aactgtaaaa ccaattgcat ggctaaatgc gatatggtat cgttggtaat tttcattact
    22441 tgttatttta gtcatgcgca tcatagtatt ttccctcctt cgtgtagttg tcttttccag
    22501 tgtacctgaa agtgtcagaa agttcaacta gcgctgacat ttacttaata tttatcgatg
    22561 tgtaattctg tcagagagct ttttttatct gactgagggt aatgtctcgt ggaccacatc
    22621 attgtattct gagctaaacc agcgttcagg cgaaatcata tggtgtttga taaaaaactt
    22681 tgtctacatg acttcctttt gccatttgct taataccgtt gcattaaaca gctcttctct
    22741 ttggaaatct caccaacatc gcgttcagga gtatcgttgt ggttgcaatg gcaagcggta
    22801 gcctttcatc aattcttgaa tgatttaccg aatgtagcac ttgccttctc gcggtacccg
    22861 aacccgaaat aaaattttag tttggcgata aaatcggtat ccgccatcga tacttcatat
    22921 tttttcgtat cagattcatc ggaaacttcc acattgacga accaataagc tttggcatgc
    22981 ttaggtcgtt ttaaaccaca ccagggcgac taatcgccct ggtgttcact tttttttatt
    23041 ttacttgaac tcattgactg ttttcccttt attttaaaca ttataaagac cgatgagtgc
    23101 ttgccgcgtc ttttgttttt atcacggttt tttgtgcaaa aaaacctgcc gatagttggc
    23161 aggtcgtcta cgtgttaagc tttggtgtgg tctttagctg gcaattggtt ttctaaatca
    23221 gttcggacaa caagcacatc gcaagctgcg tttctaataa catattcaga aacagaacca
    23281 ataaataaac gttctacagc attcaatcct gtggctccta acataatcaa atctacttga
    23341 ttatcttctg ggatttctct ggcaataatt tgttttggtg agccatattc tacgacagag
    23401 gttacattat tcaagccagc tttcttcgca ttgctttcat aatctgccaa tgtttgcttt
    23461 gccatttcag tagcttgttc tgcaagcatt ccatcaaagg atgaaacggt ttggaaagaa
    23521 cgtgtgtcaa tgacatgtgc taatagaagt tctccgttat ttcggatagc tacattgacc
    23581 gcctttttaa aagctaattc agcttcatca gaaccatcta cggcaaccat gatttttttg
    23641 tattgttgta acataagcat tcactcctca agtcaatcgt attgtactct tttattgtag
    23701 aacactttag ggaaaaagag aattatttac tgctttggtt cagcaatttt ttttgcaaat
    23761 cggatactga aggtggcaga aacaataatt acgatcagta aaaagaccaa ggcaataaag
    23821 cgttgattga aaaaagctgt taaaataccc attacgccta aaacagcata tgcagctccg
    23881 taaaattgta ggaatccttg atttttatca tttttgttga tcaatacaaa aaagatatcc
    23941 tgtttattaa ataaataata gctaacgaat aataatacta aagtggcgat aatcattaaa
    24001 atcctaatca tgaacaaatc ctccaaaata agcaacaaat agaaaaagca atttgccaag
    24061 agcaaattgc tttattcttt aaaagatggt aatctccgtt aatgccgttg tttgtcctgt
    24121 agtattgatc ccgcgaattc aagcaggtgg gtcccatggt cgtagcttcg aactaccaaa
    24181 tgataaggca tgttaccagc agtgacacaa gacgggtccc aagatatcaa taaaaatgtt
    24241 cggtcaacac acaaagagga caattcgaac atcacagatt tcccacctct atagtagcat
    24301 atgaatttaa aaagagcaat gagagagctc gcagaaacag aaccgttttc ttgtctagtt
    24361 ttttggagga tgctttttcc actcttttat acggtaatac tgttgaccca gagcctgttc
    24421 atatttccct gtatcttttg gctgataata ctgagcattc ttcagtttat ctggtaaata
    24481 ttgctgattg acccaagcct gatcaaaatg atgaggatac tgataaccca cgccacggtt
    24541 gagtgatttc gcacctttgt aatggctatc tctcaaatga tcaggaacat ctccagcttt
    24601 gccctctcga atatcagcaa gtgctgcatc taatgccatg taggcagaat tggattttgg
    24661 cgacaagcaa agatcgacga cgacgtctgc tagtgggata cgtgcctcag gcaatcccaa
    24721 tttttcagca gctaatacag cgttcactgt acgagccgca gcagcaggat tacctaaacc
    24781 tatatcttca taaccgataa ccatcagacg ccggcaaata cttgccagat cacctgcctc
    24841 aactaaacgg gcgagataat gaagtgcagc atccacatca cttccacgaa tcgatttttg
    24901 aaaagccgaa ataacgtcat aatgagcatc tccatttttg tcatgggtaa gagcttttct
    24961 ttggacacat tcttcaatga tcgacagtgt aagatgaatc cttccttctt tatctgaagg
    25021 agtcgatagt gtagccagtt ccagcccatt caatgcactc cgcaaatcgc cgttcgttgc
    25081 acgcgaaagg tgaagaagag catcttcgtc taactggatt gcttgttgtc caaggccgcg
    25141 ttctttatcc cttaaagcat gttcgactgc caactggatg tcttgttctg ttaatggttt
    25201 gacttcaaag atctgtgtac ggctgcggat cgctggattg atggtaatat aaggattctc
    25261 agtagtcgct ccgatcaaga tgattcggcc actttctaaa tgaggaagca gaaaatcctg
    25321 ttttgtttta tctaatcggt gtacttcatc caataagagg atcactgtac cactcatttt
    25381 tgcttcttcc gctacaactt gtagatcctt ttttgaatct gtagcggcgt ttagcatgcg
    25441 aaatgcataa ttagtagagc cggcgatcgc acttgcgata ctcgtttttc cagtaccagg
    25501 aggtccatat agaatcatgg aagagagcat gcgagcatcg accatgcggc gtatgatttt
    25561 tccttccccg actagatgct gctgaccaac gacttcatcg atcgttcgag gtctcatgcg
    25621 ataagccaat ggctgttgca taggattttc ctccttcttg tttttacgtc ccttaattat
    25681 atcatattta gcaagatgcc tagaaagaac agccttctgt tgttttgttt tcatagctta
    25741 tccaactaat attgtgtatg ataaagtagg tgagaaaaat gacggataaa caaaagatag
    25801 atgaattttt ttggaataaa acgacgccag aagtggcacg ggatttattg gggatgtatt
    25861 tggagcatga cacaccagaa ggaagattag ctggatatat tgtagacgcg gaagcatacc
    25921 taggtccaga agacgaggcc gcccatagct atggtttaag aagaacaccg cgtgtgcggg
    25981 caatgtatga aaaaccaggt acgatctacc tttacacgat gcatactcat cggattttga
    26041 acatcattac tcagccagaa gggattcctc aaggagtaat gatacgtgca atcgaacccg
    26101 ccgcaatgat tgatcaaatg agtaaaaatc gtggcggaaa aactggtcca gatatcagca
    26161 acggtccggg aaaattagtg gaagcattag caattcctca agaactttat ggtcagtcta
    26221 tagcagatag ttcgttacgc ttagtttttg agaaaaaaaa gacaccaaaa aagatcaatg
    26281 ctttgcctag aatcggtatc ccaaacaaag gtgtatggac agaaaagccg ttgcgttttg
    26341 ttgtgtcggg aaatccttat atctctttac aaagaaaaaa ccaaatcgag aaagattggg
    26401 gttggaggaa agaaaatgaa aaagaaggat ctattaacat atttagatga aataattgaa
    26461 aaacaagtga cagactatga tgtagcttta gattgggata caaaaaatca tacgatcgag
    26521 atcgttgttc gcttgtttgc agaaaataaa gcacaatttg agattgatga tgcagaaggg
    26581 atcgtttcgc aagaaccaat tatcgaattt gaagacggcg tacttctttt taatcctcag
    26641 aaatctgttt ttgatgaacg caattatcta gcagtaatcc cttatgaagg aaaaaaaggg
    26701 ttggcaaaat cagtagctga ttctttagtg gaatatctaa atgaagtatt agctcaaggg
    26761 caaagcgatt tgcttgattt tttggatgaa gacgatgacg aagctgtatt cgagcttcac
    26821 tgggataatc agcaactagc agaattagta gaagtaaaac agcaaaatga agcagatact
    26881 tatttaccgt acccaagtta ttaaggagtg acagatatga aatggaatga agtaaaagta
    26941 gaaactgaaa gtgaagcagt cgaagcagtt gctaatattt tgatggaggc aggggcgagt
    27001 ggtgtcgcga ttgaagattc tttagatgtt gaacattttc agtcggatca atatggtgag
    27061 attttagata aaaatgattt tacatcgctg aaagaaggcg cactagtcat ggcttatttt
    27121 ccggaaacga ctttcttacc ggaaatcttg ccttttatca aagatcgtat cacacaatta
    27181 cctgagtttg gtctagctat tggaaaaaat aaagtaacag ttagtgaagt agaggaaagc
    27241 aactgggcaa cagcctggaa gaaatactat catccggtgc gtattacgcg aatgctgacg
    27301 gttgcgccaa gctgggaaaa ctaccagaca gaagatcctc tagaaaaaat tatcacacta
    27361 gatcctggta tggcatttgg cacaggcact catcccacga cttccttgac attgacagca
    27421 ctagaaacag tcttgcgtgg tggtgaaaca ttgctagatg tgggaactgg gtcgggcgtg
    27481 ctaagtattg cagcaaaata tcttggtgca aaagatgtct atgcctttga tttagacgaa
    27541 gtagccgttc gttcggcgaa agaaaatatg gatatgaatg aaatagccaa agatgtccat
    27601 gtatcagcta acgatctttt gacaggaatc gagattgaaa gtgatgtaat cgttgcgaac
    27661 attttagcag atatcatctt attgatgatc ccagatgctt ggcgtttact gaagcaaaca
    27721 ggcacgctga tcgtttcagg aatcattgaa gagaagaagc aaatggttct ggatgccatg
    27781 ttagaacaag ggtttatcgt ggatcaaatt ttccagcaaa aagactggta tgcaattatt
    27841 ttgaaaaaac cagaggtgga atagatgcag cggtattttt taaaagaatc atatgaatca
    27901 aaagactatt ttgttttaga aggggaaaac tatcaccaca ttgtacgggt tatgaggatg
    27961 gaacccgagg atgaagtttt tttggcattc aatgatcaaa tagccattgt tgcagaaatc
    28021 acggagatta ctgaagaggc tgtttatctg aaagaaatta aaaaagaaga aaatcaaaag
    28081 gaactgccaa gttcgattac catagcgagt ggctatccta aaggggaaaa gctggattgg
    28141 atcgttcaga aatccactga attaggagcg caagcttttc ttggtttccc ggcatcagcc
    28201 tctgtcgtta aatgggatgc aaaaaaacgt atgaaacgac aacaacggct tgaaaagatt
    28261 gctcaagagg cagcggaaca atcccatcgg cagatcgtgc catcagtaga gttattggaa
    28321 acgaaacaag atttatttga gcggctagat gattatgacg tggtcttggt tgcttatgag
    28381 gaatcagcaa agcaaggcga aaaatcaaat gcagccaaaa ttcttagtga aacaaaacca
    28441 gatagtcgaa tcttagctgt ctttggacca gaaggtggat tatcccgcga tgaagtcgat
    28501 tattttgtag aacacaaagg gatactttgt ggattaggac caagaatcct tcgtacagaa
    28561 acagcacctc tttatttatt aagtgctgct agctatcaat gggaattaat gaatccttct
    28621 ttcagctaac gttcttcatt aataaaagag tatcgacaga aagaattgaa aaaatagtca
    28681 acaatttgta taatagaaac aattatttat acaatggata cgaaaaatcg tttgaaaaag
    28741 cttttaaaaa gcgaacattt ccgtaaaaaa acgtataact gcacggattt tgggacaatt
    28801 atcaaacaga tatttgtccc gattcccgtt ttaccgaaaa aagaaagggg gatcgattcc
    28861 tgtgacaaaa gaagaaattt taacgggacc cggtgtcata aagctcgtta gtcaatacat
    28921 gggtcccgaa catgtagcat ttgttgagaa agcatgcgaa tacgcgactg ctgcacatga
    28981 cggacagttt agaaaatcag gcgaacccta cattattcat cctatccaag tcgcaggtat
    29041 attagcagat ttaaaaatgg atccccatac agtggctaca ggcttcttac atgatgttgt
    29101 tgaagataca gaaatcactt tagaagatct gagagaagaa tttggcgatg acgttgctat
    29161 gttagtggac ggcgtaacca aattagggaa aataaaatat aaatcccacg aagagcagct
    29221 agcagaaaac caccgaaaga tgctgctagc aatggctcaa gatttacgag ttatcatggt
    29281 caaattagct gatagactac ataacatgcg tacgttgaag cacctgcgag aagataagca
    29341 aagaaggatc gctcaggaaa ctttagaaat ctatgcgcct cttgcacatc gtctagggat
    29401 cagccggatc aaatgggaat tagaagatac agcacttcgt tatctaaatc caaaacagta
    29461 ttaccgtatc gtccacttga tgcagacgaa gagagaagaa cgtgaaaaat acgtaagtgg
    29521 tactgttgaa gatattcgaa tagcgacaga agagctgggg atttttgcag aaatctatgg
    29581 acggccaaaa cacatttatt caatctatcg taaaatgaaa gatcagaaaa agcagttcaa
    29641 cgaaatttat gacttgctag ctattcgagt gatcgtagat tcaatcaagg attgttatgc
    29701 tgtattagga gcaatccaca caaaatggaa accaatgccc ggcagattta aggattatat
    29761 tgccatgcca aaagctaata tgtaccaatc tttgcatact accgtgatcg gtccggcagg
    29821 aaatccggta gaaattcaaa tccgaacaca ggaaatgcat gaaatcgctg aattcggggt
    29881 tgctgcacac tgggcctata aagaaggaaa aaatgaaaaa gtagaaccag atggtatgac
    29941 gaaacaatta agctggttcc atgagatact cgaacttcaa gacgaaagct atgatgcttc
    30001 tgaatttatg gaaggcgtaa aaggagatat ctttagtgat aaagtctacg tcttcacacc
    30061 aaaaggagac gttactgagt taccaaaagg atccggacca ttggactttg catacagtat
    30121 ccatacagat atcggtaaca aaaccactgg tgcaaaagta aatggcaaaa tggtgcagct
    30181 tgattacaaa ttgaaaaacg gagatatcat tgagatcatg acttctccaa attcatttgg
    30241 cccaagtcgc gactggttga aattagttgc tactagcaaa gcaagaaata agatcaaacg
    30301 tttcttcaaa gcccaagatc gagaagaaaa tgtgatcaaa ggccacgaat ccgtggtcaa
    30361 atgtattaca gatctaggat ttacgcctaa agatattttg acgaagaaca aactgcaaga
    30421 agcactcgat cgttttaatt atcaaacaga agatgatctc tatgcagctg tagggtatgg
    30481 agaagttagc cccttgacga tggccaatcg tctgactgaa aaagaacgta aagaacaaaa
    30541 aatcgagcag caaaagcaag aagcagaaga aatcatgaat cagccgaaaa aagaacctga
    30601 caaaatgaaa gtacgtcatg aaggtggcgt tgtcattcaa ggggtagaaa acttactaat
    30661 tcgtatcagt cgctgctgta atccgattcc tggtgatgat atcgttggtt atatcactaa
    30721 aggcagaggg atatccattc atcgtcgaga ttgtccgaat gttcagcctg acaaaccaaa
    30781 tgtagcagaa cgtttgattg aagtcgaatg ggaagataca tcgaatacac gaaaagagta
    30841 tgatgcagat ttggaaattt acggctataa tcgttcaggc ttattgaatg atgtacttca
    30901 aacagtcaat gcgctaacga aaaatctcaa cagcgttgaa gcacggacga ataaagataa
    30961 aatggcgacg atccatttga cggttggtat ccagaattta tcccacctaa agagtatcgt
    31021 ggataaaatc aaagcagtac ctgatgtcta cagtgtacgc cggacgaatg gataggagga
    31081 cagatatgag agcagttgtt caacgggtaa gtaaagctgc tgtgtcagtc gatcagcaag
    31141 aaattggaaa aattggctac ggcctacttg ttttattagg cattcatgaa accgatacgc
    31201 aaaaagatgt ggactatctg gtaaaaaaaa ttgcacaaat gcgtattttt gaagatgaac
    31261 aaggaaaaat gaatattagt gtagaagatg tgggaggaca aattttaagt atctctcaat
    31321 tcacactgtt tgcggatacg aaaaaaggaa atcgaccaag ctttgtggaa gccgcacgac
    31381 cagaaacagc tattccttta tacgaatcat tcaatgaagg aatcagaagt cgcggcatca
    31441 cagtcgctac cggacaattc ggagcagata tgtcggtttc tctcgtcaat gatggacctg
    31501 taacgatcat tattgattct aaaaacaaat aaagtaatct ggttttcact agtaaaaact
    31561 atgaaacacg tcgcttaagt tagactgata aactctaact taagtggctt ttttgtctgc
    31621 aaaaagcgct tcttttgtca gcagtcggaa aaaaagttaa gcttaaatta tacaagttat
    31681 tttttatagg aggttgctga agatgaaaga agtaccagta gtagatatca aaatcacaga
    31741 tttaaaaaaa ttgtatcaaa aaggatacaa catgttggag gagttacgcc atgaagcgga
    31801 cgctcccgtc gtcaaagcaa agattttcaa taaagaagct atcacgatct acggatcatc
    31861 agcagcaaaa gtattttatg atccaaggaa tttcaaacga aaaggtgcta tgccaaaatt
    31921 agtattgaag acactttttg gtcaaggtgg agtacagact ttggatggag ctgctcatca
    31981 ccatcggaaa aatattttta tggatctaat gacaccggaa agaatggaag attatcatcg
    32041 tatcttagat aagaatctga cacaagcact tgaagcacag catggtcaat ttgaactatt
    32101 tgatttaagt aagatggttt tctttacatc tatttgcgag tgggcaggta tcaatttatc
    32161 agcgatttct aaagatgagg tcgaaaaatt agcagaatat caaatctcta tgatcagcgg
    32221 aacgtttact tctccgattg atcacataaa aggtgtcgaa aatcgcaaaa aatcagaaaa
    32281 atgggcgcaa ggcttgattg aagaagctcg acaaaatcct gtggctggaa aagaaaatgt
    32341 ggcattatat gcttttgcca atgccacaga tttagatggt cagcttttac ctctggaagt
    32401 ggcagctgtt gaattattga acatcatccg tccaacggtt gctctaactg tgtgggcggc
    32461 attgatggga catgcacttt tcagtagacc agatttgtat cagcaattaa aaaatgactt
    32521 ttcgacgtta caagatccgt ttattcaaga aatgagacgc tattatcctt tcttcccaat
    32581 gctacctgcg atttcgctga aagaagtaga agtagatggt tatcgtatac cagaaggcag
    32641 ttgggttatt ttagatttat atggaacaga ccatgatgaa cgaacagttg aagcgccaga
    32701 ttcctttatg atcaaacggt atgtagggaa agcaaaagat atttcctata aagaagaata
    32761 tgagatgatt gcacaaggcg ggggaaattt tcgacaaatg catcgctgtg caggagaatg
    32821 gatcactctt catagtctac gtgtgttttc cgatcagcta gtaaataagt ttgagttctc
    32881 tgtacctgaa caagattgga cgattccatt caatcagttt ccgacttatc cgaatagccg
    32941 agcgctactt tacaagaatt agattagcat aaacttatcg aaaaaaagga agatcaaaca
    33001 gtgctgatct tccttttttc atattactat aaattgataa ggtcaattga tcaaaatcca
    33061 cgaaatgctt catcaatggt ttgataatct tcattggata aatgcaaatc taaagcttgg
    33121 acattgtcag atacttgctc tggacgctta gctccaggta taactacaga cactcgtgga
    33181 ttcttcatgt accaagctaa aacaagttgc gcaggagtgg cttggtatct tttagcaatg
    33241 gggcgaagta catcgacagc ggttaaaatt tctctgaaac gttctccttg gaaatctgga
    33301 tttctttttc gaggatctcc ttcaccaaat tgtttttcct caccaagttc atattttcct
    33361 gtcagcaaac cggaagcaag aggaaaatag ggaacaaaac tgattttgtt tttttcaaga
    33421 taagggaata attctttttc agcctgccga tggatcaaac tgtatttatc ttctactaca
    33481 tcgacatatc catctgcatt cgcttctttc aattgttcta acgtgaagtt tgacacaccg
    33541 actgctcgga tttttcctgc ttcttttaat tcgtgcaatg tagcaactga ttcatttttt
    33601 ggtgtgcttt catccggaaa gtggatataa aaaatgtcga tgtaatcagt ttgcagacgt
    33661 tttagtgcat cttccaccga ttgtttgaga aactcaggag aattatcaaa tgttcgccct
    33721 ttatttggta catgagcagc ttttgtagcg atgacgacac gtgagcgatc atattctttt
    33781 tccttcagta cctcgccaat cagttcttcg gaacggccgt ttccgtaagc ataagcggta
    33841 tcgatcaaat tgatcccgct attcaaagct gtacgaacta cttgttttcc tgtctcatct
    33901 tccaaaccgt caaaaagatt atgtcctcca acggcatttg ctcctaatcc aagctgttct
    33961 gcgtagactt gtgaatgtcc gattcttatt tctgccatag ctgattcctc ctttttgtct
    34021 agtctaaaac aaaataccaa aaagctgaac taaaacgttt ttgtgagggg aaatagtcag
    34081 atatctcttg actttgagac tggtttttag taggttataa gtaatatcga atgtcaatga
    34141 aagagcgagt aagtttttga aactttacaa gagaggatca tcgaggctga aaatgatcta
    34201 aagtttcatt agcctaaaga cactctggag atggacgagt gaaaagccgt cccgcgtacg
    34261 agcgttattc gtaagaggaa agagaaatct ttcaaaatta ggtggtaccg tgtatgaatt
    34321 tcattcaccc ttgttcatga tgaacaaggg tgattttttt atttacatag ctaagaaagg
    34381 gagagaaaac tatgagtttc caaagaccaa aaggaacaaa tgatattttg ccaggcgaat
    34441 cagaaaaatg gcagtttgtc gaagaaacag cacgtttatt attcagagat tatcagtata
    34501 atgaaatgcg tacaccgatt tttgaacact atgaagtgat atcccgaagt gtaggggata
    34561 caacggatat cgtttcaaaa gagatgtatg atttttatga caaaggggaa cgccatgtga
    34621 cgcttcgacc agaaggtaca gcaccaatcg ttcgttcttt tgttgagcat aaattgttcg
    34681 gcccagagta tgcgaaaccg ttcaaaactt tttatatggg accaatgttt cgctacgaac
    34741 gccctcaaaa aggacggtta cgtcaattcc atcaaatcgg agtagaagca tttggatcag
    34801 aaaaccctgc aactgatgtg gagagcatgg tgatggcact tgatttcttc aaacagttgg
    34861 ggatcacgca aatccggcta gtcatcaatt cattaggtga taaagaaacg cgtcaagcct
    34921 atcgtcaagc attgatcgac tatcttttac cttttgaatc agagctgagt gaagactcta
    34981 aacgccgcct tcatgaaaat ccattacgcg tattggacag taaagacaaa cgagatcaaa
    35041 aatttgtggc agaagctcca tctatcttag attatctaag cgagcctgct caaaaacatt
    35101 ttgataccgt tgtttccatg ctggatgcat tggctgttcc ttatgaaatc gacagtaata
    35161 tggttcgcgg actagattat tacacacata cgatttttga aatcatgagt gacgcaccaa
    35221 aaatgggggc acaagcaacg atttgcgcag gcggacgtta tgacaatcta gtggaagaac
    35281 taggtggtcc ttcaacacct ggcttcggat ttgcaatggg tatcgagcga ttactcttga
    35341 ctatggaggc agaggaagta gttatccctg catttaatga gttggatgct tacgtggtcg
    35401 cactcggaga cgaaacaaat atcgaagcat tgaaagtggt ccaagcgatt cgaaactttg
    35461 gtttttcagc tgaccgcgac ttcatgaacc gtaaagcaaa agcgcaattt aagacagcag
    35521 ataaagcgaa tgccaaatta gtattggtga tcggtgaaga tgagcttgct agtcaaacga
    35581 ttaatgtcaa agcaatggca agccgaaaag aaaaaagttt tccattagaa gagatatatg
    35641 aacattttga tgaagtctat gatgaaatga ctttattcga gtaggaggaa aaaaataaat
    35701 ggcacaaaga acgatctatt gcggattagt ttccgaacaa caattagaac aaacagttac
    35761 attaaaagga tgggtacaaa aaagacgaga ccttggggga gtaatcttta tcgacctacg
    35821 tgatcgtgaa ggaatcgttc aaatcgtctt taatcctgaa atcaacaaag aagcatggga
    35881 aatcgcggat aaatgtcgca gtgaatacgt gatcgaagtg actggggaag ttagaagacg
    35941 cggagaaaat gcagtaaatc ctaaaatggc cactggtgaa ttcgaagtga ttgcaagtga
    36001 aatcgtgatt ttgaatcaag caaaaacccc gccatttcaa attgaagatg atcaagcaat
    36061 cagtgacgaa atacgtttga aatatcgtta tttggatctg cgtcgtccaa aaatgactaa
    36121 aaacatcaaa ttaagaaatg cgacaacaaa agcgattcgt cattatttag atgataatga
    36181 ctttctcgat atcgaaacac cttacctagg gaaatctaca ccagaaggcg caagagacta
    36241 cctagttcct tcacgtgtcc atgctggaca tttctatgcg ttgccgcaat ctcctcaatt
    36301 gtttaaacaa ttgctgatga atgctggatt cgaccgttat tatcaaatcg ttcgttgttt
    36361 ccgcgatgaa gatttacgtg gagaccgtca gcctgaattt acgcaagtag atattgaaac
    36421 aacgttctta acagcagaag aaatccaaac ttacacagag ggattgattg cgaaggtaat
    36481 gaaagaagtt cgcggtatcg aagtcacatt gccatttcct cgtatgacct atgatgaagc
    36541 aatggctcgc tacggcagcg acaaaccgga tacacgtttt gatatggaat tgatcgatct
    36601 aagcgataca gtcaaagaag tagaattcaa agtcttccaa atggctcttg aaaacggcgg
    36661 tgtcgtgaaa gcattgaatg ctaaaggcgc tgcagatcgc tactcacgaa aagacatgga
    36721 tcagttaggt caatatgtag gacaattcgg agctaagggg cttgcatggc tgaaagtaga
    36781 agaagacgga ttgaaaggtc caattgctaa attcatggga gaagcaacag aagcaatcat
    36841 taaagcaaca gatgcaaaac caggtgactt gctgatgttt ggcgcagata aaagtgaaat
    36901 cgtcgctgct gcattaggtg ctatccggac acgtttagga aaagaattag gactaattga
    36961 tgaatcgaaa ttcaatttct tatgggtaac tgattggcca caatttgaat tttcagaaga
    37021 agaaggtcga tatgtctctg ctcaccatcc attcacgatg ccaaaagaag aagatatccc
    37081 gttattggct gaagaccctg caaaagtcta tgcagaagct tatgatatcg tcttgaacgg
    37141 ttatgaacta ggcggaggtt ctatccgtat tcatactcgt gagcttcaag aaaaagtact
    37201 agaaactcta ggctttacaa aagaacaaat ggaagaacaa tttggtttct tgttaacagc
    37261 tttagattac ggattccctc cacatggcgg tattgcatta ggattggacc gttttgcaat
    37321 gttattagcc ggagaagata atattcgtga agtcattgcc tttcctaaaa acggaaaagc
    37381 agcagatgtg atgagtgatg caccaagtat ggtttcgcct ttacaattgt tcgaattgaa
    37441 tattgatgta acgaatatag aagaatagcc gataaaaagg taaaaaggat ttgtcgaata
    37501 cgcttctttt gacaaccttg atttaattga aaaacacttt ataatgaagc cataacatat
    37561 aaagcggggc ttttccatga aaaaaacatt cgaaaattgg gcttatcatg attacacgac
    37621 caagctttcg gtatctatcg tttatgcgtt gatcgcatca gtcgcattga atttctttta
    37681 tcagccagga catatttatt caagtggtat tactggtcta gctcagattt tgacgacttt
    37741 gtcaaaatca ctgataggat tcgaagtacc ggtatcaaca actttgtatt tactgaatat
    37801 acctttgttt gtattggcgt ggttaaaaat cgggaagaag tttacaattt tcacttttat
    37861 cacagtcact ttgacctccc tttgtatcca tattttaccg gaaacagtcc tatctaccga
    37921 tccaatcatc tgtgcgttgt ttgggggggc tgtgatggga ctaggtattg ggctagcgtt
    37981 gagaaacggc ctatcatcgg gcggtctaga tatcgtgagt atcacgattc gtaaaaaaac
    38041 aggaaagtcg attggttcta tttcgatcta cttcaatgca ttgatcgttt ttgttgccgg
    38101 aattctattt ggctggcaat atatgtttta cagtacgttg tctattttcg tcagtggaaa
    38161 agtaactgat gtcgtttata ctaaacaaaa gaagatgcag gtaatgatcg taacgaaaca
    38221 tccagaaaaa gtagtaaatg aaatccaaaa ctgtatgcgt agagggatca cgatcatcaa
    38281 tgaagcagaa ggtgcttata agcatgataa gcagacagta ttgatgacga tcgttacacg
    38341 atacgaactg cctctactta gagaagcaat gaaagcttct gatccacaag cttttgtaag
    38401 tattgcagaa aacgtccaga tacttggtcg tttctatgag gatggcattt agatcgagtt
    38461 tgacaaaaat tcatgaaata ctgctgtaga cagacgttcg tttgtctgca gtttttttct
    38521 accatagtag aatcagcaaa gaaaaactgc ttttttattt gaaattttct taaagttgaa
    38581 aaaaagcatg tttatttttt taaatttatg ggatgataga aaaaaatagt agagaaagga
    38641 cacgattcta cttgctattt tcacttaata cccgtatatt acgaatgaaa aaagtttatg
    38701 aaaagttgga atattgaatg gaaaaacaaa aaaggttaaa aaacagccgc tacatcactg
    38761 ggtttgatgg gctccgttca ttagctgtgg tgggtgttat cttctatcac cttctgccaa
    38821 ctagtatgaa aggcggctat ttgggcgttc cgattttttt cgtcgtatct ggttatttga
    38881 tcacggatct gctgcggcag gaatgggaac aaaatggaaa aatcaatatt tggcaatttt
    38941 atgtcagaag aatgaagaga ctctatccgg cacttgtttt tatgcttgtg acagcttcgg
    39001 cttatatcac tttgtttcag cgagggctgt taaacaatct tcgaggagtt gtggtcagca
    39061 gcttactgta tgtcaacaac tggtggcaaa tcaataacgg tttatcttat ttcgatcgat
    39121 ttgctaatga gtcaccgttt acgcatatct ggtctttagc agtagaaggt caaaactatt
    39181 tggtttggcc gatcttgttt gtattgttga tggtatttgt tcgtaaaaga aaatggatct
    39241 tttatactgt gttaggtaca tccttcctct ccgccatctt gatgatgatc ttgtatacac
    39301 caggaggcga tccaacccgt gtttattacg gtaccgacac tcgtctgttt tctatatggt
    39361 tgggaagtgc gcttgcgttt gtttggccaa gtacgcgttt aaagaaaaac atcccgaaac
    39421 aagcgaaaaa tattctgaat atcgctggag gaatctcgct tttggcactg attctttgct
    39481 tcttcttttt agatgatcat tacaatttca tctattacgg aggaatgctc ctagtgagtc
    39541 tgttatgcgt agtgctagtt gcagtgacag cacaccccgg agcaagtttg aatcgatggc
    39601 tgacaaatcc agtatttact tggattggca aacgaagcta tggcatttat ctgtatcagt
    39661 ttccagttat gattttttat gaagcaaagg tgactgatat tgccgatcat gttttgttgc
    39721 atactttagt ggaaatcaca ttgattctaa tcatcagtga actttcttat cgatatgtgg
    39781 agcgtccgct agcacgtttt gattatggtc gtacattcca agtgatgaaa ggatggttca
    39841 gcaaaccgat catcagtaag agcaaaccat gggttgttcc aggcacatta cttgtagcaa
    39901 ttgcactcgt tggtttcgtt acggcaccga aaaacgctgt aacagccgat caaaaacaac
    39961 tgcaggcaaa aatcgctgaa agtaaaaaat tagcagaaga aaccagaaac aattcagaaa
    40021 acaccactag ttcggttgac caagcgatcc ttgataagta cgagttgtcc aaggcacaag
    40081 ggaataaggc acaaaaatta gaactaacgg catttggtga ttcagttatg ttggatgctg
    40141 caacggatct acaagaactt tatccaaaag tagtggtaga tggagatgtc ggacgacaat
    40201 tatatgccag tgtaccgtac attgaaaaat taaaggaaca agatttgttg aaagacactg
    40261 ttctgatcgg actaggaacg aatggctcat tctcggaagc tcagtttgat agtgtgatgg
    40321 atgcaatcgg tgatcgaaaa gtatactggg tcaatgtccg agtaccgacg aagcgctggc
    40381 aaaatgacgt gaatgcgatg cttgaacaga tgacgaaaaa atacgacaat atgacgctga
    40441 ttgattggta cagctacagt aatgaccata gtgactggtt ttatgatgat caagtccatc
    40501 caaatcctga agggatgaca cattacattc acttagtcag cgaagccttg ctaggaggaa
    40561 gttcaagtac gaacagcagt gagacaaacg tgacagaatg atagctacaa aaaacttcag
    40621 gcaaaaacat ttgtttttgc ctgaagtttt tttatattta ccgtgttttt aacattcata
    40681 tagattcagg aagcaaaacg gttgagtgtg tctgtaaata acgttctttt tgttttcggg
    40741 tgagtttttt gaatgcagct tctgcttttg ttgcttcgct tcttgaagaa aatacttctg
    40801 catggatcat cttaacaggt cttctttttt gaagacgagt atatttagca ccagtaccat
    40861 tgttatgttc ctctaatcgt ctagaaagat cggttgtata tccaccataa aatgtctgat
    40921 ctttacagaa taatacgtaa aaataatgtt catttgccat agagcatctc acgaatctct
    40981 gcagtgtatt ctccatccat attgtaagtg atcaatggtg gagcgacttt gaatccgtct
    41041 aatttgcctt ctttgattcc ttcaactaac aaaatatttg cctcttttcc aggttttggg
    41101 taaacaaatt ggatcctttt aggtgcaatt cgatagtctt ccatgacctg aatgatttct
    41161 aagaaccgat caggacgatg gaccatagta aagcgtccat ttgtttttag tagcttactt
    41221 gaaatatcga tcacttcttc taacgtcgta tgaatctcat gacgggcgat tgccaggtgt
    41281 ttattagggt tcgttttatt tgttggcagc cctttgaaat aaggtggatt acagacgact
    41341 aagtcacaag aattatgtcg aacgacagat aagctgtctt tcagatcgat ggtgtggaca
    41401 gtcatttgtt catttaaatg gttaagcgcg atactacgtc ttgccatgtc tgctagacga
    41461 ggttgaagtt cgatagcgtc gatagccgcc tgggttcgtt tacttaaaaa aagtccaact
    41521 gcgccatttc ctgcacaaag gtcaacaatc ttcccttttt taggtacacg ggaaaagtga
    41581 gcaagcagca cagcatctaa agaaaaagaa aacactttgg aactttggat aatcttgata
    41641 tcttctgcgt ataattggtc gattcgttcg tcttcgtgaa gcatggattc cctccaaaat
    41701 gaaaaaaata gtcgtctgta ttatactatt tttattaaaa agaaccaagg tggcttgtca
    41761 aacctctatt ttttatgcat actatgacca taagaaggga aaggaagaga ctatgtttta
    41821 tcgatttatg cgcggggtag ttcgagtact attagcctta gttaatggaa atgcccgcta
    41881 tgagaataaa gaagttttac cgcaagatga gaattacata ctagttgctc cccatcgtac
    41941 ttggtgggac ccattatatc tggcggtagc tgcagcaccc aaggaattca gctttatggc
    42001 aaaagaagaa ctgttcaaaa atccagttct gcgatttata ttaactcgat ccaatgcttt
    42061 tccagttaaa agagaaaatc caggaccaag cgtcattaaa acacctgtaa agattttgaa
    42121 atcgacaaat ttgagtttga tcatgtttcc cagtgggaca agacatgcta cagaacttaa
    42181 aggcggaatg gcattgattt ccagaatgtc taaagcgaaa atcgtgccag ctgtatatca
    42241 aggtccgttg acacttgggg atctgttcaa gcgtcagaga gtgacagtgc gtttcggcga
    42301 accaatcgac ttatcagata tcaagaaaac ggataaagaa ggtatggcgg agatcgaacg
    42361 acgaacacaa gcagcttttg acaaattgga caaagaagtg aaccctgatt tcaaatatga
    42421 agtcaaaaaa aaataaaatg ataagtttat gcgtgggcgg tcttttgaat ctactgtcct
    42481 gttcagttca gactttttca atgaaaacga gggagagacg tcaagtctgc cctctttttg
    42541 cgttccaata tgtaaaatag aagacaggtg tcatgtaatg gatcaaagtg ttataataat
    42601 tagaaggaca ttttttgagg tgattgaatg cgttttttac atacagccga ttggcatatc
    42661 ggaaaaaaac tacaaggtta tgacttgatt gaagaccagc gtcatgtact atcttctata
    42721 ctagatatcg cgaaagaaga aaaagtagag gcaatcgtga ttgccggaga cttatatgat
    42781 aggagtgtcc cagctgttga tgccgtagaa ttatacaatg aattgatggt cgattggaat
    42841 ttaaaggaaa agatcccgat ttttgctata tcaggaaatc acgatagcag tacgagactt
    42901 tcagcgggta cgccatggtt cagttattcg aactattttt tgcgaacaca gctagcagaa
    42961 gcatttgaac cagtcgagtt taaaaacgta caatttttct ttcttcctta ctttgaacca
    43021 attgcagcga gattatattt tgaagatgac aatatccgaa cgatcaagca agcggtagcc
    43081 aaggtgatag ataaaatgca ggagctattt gattcagaaa aaaaacaagt attgattagc
    43141 catttttttg taatgggaag tgtaaaatct gattcggaaa cgaaaatcga agtaggcggg
    43201 ttggattcga tccctggtga actacttaca ccgtttgatt atgtagcact aggacatttg
    43261 cataatcaag ctgctttgag acaggataat gctcgctata gcggttcccc attgaaattc
    43321 tctttatctg agatcaacca aaaaaaaggc gtatggatcg ttgacctgac aacaggcctg
    43381 gagttgaatt ttagggagat cgaacccctg catgatattg aagaaataac agcgagtttc
    43441 aaagaattgc tggatcctgc cttttatcag tccgtcaatc gggagaatta catctatatc
    43501 tatctcactg atagaacgat catcccgaat atgatgaacc agcttcggca aatatatcct
    43561 cgtattttgg ggattgaacg ggcaaacggt cgagcagaac aaacaaagcg caaacgaaaa
    43621 gtactagaaa tgaaagatcc tcaaaaatta gcagaagcat tttttagtga aatgacggaa
    43681 gagcagctaa ctgcaaacca agaaaaatgg ctcggagaaa cgttgcaaga aatcatggaa
    43741 atggaatgat caagaatgaa accgaaaaaa ttacaactta aaaattttgg tccttttatc
    43801 aatgagaccg tagatttttc acgtttgaca gaagcaccgc tttttttgat cagcgggaag
    43861 acaggagctg gcaaaacgac gatttttgat gggatcacct tcgcactttt tggtgaaact
    43921 tccggaagac ttcgctctgg aaaagagatg cgttctttgt ttgctacacc agacgaagaa
    43981 acaagcgtga ctttttcttt tgaacatcag cagatgagct atgaaatcga acgaaaacca
    44041 gaacaagttt tagcaaaacg aaaaggaaac ggaacaaaaa aacaggcagc caaagtaacg
    44101 ttgaccattt ttgatgggaa aggaaaagaa ataaaacaaa tcacaaaacg tacagaagca
    44161 gatcagttga tcagagaact gatgaatctc gatgcgaaac agttttctca aatcgtttta
    44221 ctaccccaag gagaattccg taattttttg gtttcatcaa gcagtgataa agagactgtt
    44281 ttacgtcatt tattcggtac tcaatttttc caacagttca atgagcgatt aaaagaaaaa
    44341 gcaaaacaac agcaacaaaa attggatcat ttagagcaag agctgcatct tctgcaaaaa
    44401 aggtttgttt tagtcaaaga agaggaacca gcagcagggt ttgaagccgt tttagcccaa
    44461 tggacaaacc atcaggcaat actcgaaaaa caaatcacgg aagaaaaaga agctctaacg
    44521 gaacttcagg aaaaacaaaa gcaactagaa aattcctact atcgttttga aagcttgaaa
    44581 aaaagttatg aagaaaaaac gatattaata aacaagcaaa aggaattagc tgctcaaaaa
    44641 gacgaaatag aggaaaagaa acgatggatt gggtattatg aatttacgca acgattagtc
    44701 gaaccaatcc agcgcctaca agaaataaaa gttgaaaaaa tagaactgtc acaaaaaagt
    44761 agtgagcaaa caaagaaact agcgatgact acgcagaatt atgaagagtg gcaaaaaaat
    44821 gaagcaaacc gcgtacgccg acaaacaaaa atcaaagaag ctgctcaaaa actgcaaaac
    44881 aaacagatga tggtgccaat tgtcgaatcc ttacgtagta aaaaacaaga cgcagaacaa
    44941 ttaacaattt cattggataa agaagaattg aatgccgaga agctgtatca aaatctcgct
    45001 atctatcagg aaagaactcg aattcttcaa gaaaatttac tggatcagga aaaactgcaa
    45061 gaagatcgtc ttgcttttgg acagttaaaa tatctagatg aaaagcagca agaaatagaa
    45121 aaacaaaaag caaaagacac tgggtcgctc gcacagataa gagaagaact caaaaaagca
    45181 actgaagaaa taacaaaaaa tagtcagcta ttgaaagaac agacagaaca agcagaacag
    45241 ctgaaaagcc aatgggcaaa gcagcaaatt gctcgtcttc agcttttttt gcgtccaggg
    45301 gagccatgtc ttgtttgtgg atcaacggaa caccccatac aacaattaaa acacgaacag
    45361 ccaaataagg aagatatctt aaaagtagaa caacaactgg cagaagctga gtctgctgta
    45421 gaaagaactc agcaaatcat tgccaaaagt gaagcacatg aaaataatct tcaacaacaa
    45481 ttatcagatt tagaacaaaa aatcatggca acgaatatac aattggctga acaagaagaa
    45541 cacttgcaac aggctttgaa aaaggcattt cctgagataa cagaacaaaa aacaaaaaca
    45601 actcttgtag taatcgaaca atctttagat agacgaaaaa gcaagatggc caatgcaaga
    45661 atagagctgg aagaaataag acaacaagca gaaaagctcg aacaacaatt agctgtctac
    45721 aaacagcagc agacagaaaa aaataatcag ctccagcaac tgcaaggaga aatcaaaagt
    45781 ttagaacaac aattgcaaga tacggatagt aactgtttag aagaggagat acgacgactt
    45841 gaaaaacagt tagaagaaga ctcgaatcaa ctagctgttg agcagaaagc aggagtgcaa
    45901 ttgttgcaag atcttcatac gcttcggaca cagcttgatc ttcaacatga acaaatagaa
    45961 aaaatagatg aaaaaataga aaaggaacaa gaaagaatag ctaaaaagtt agaaagccag
    46021 tcgtattttt caacaattga agaaatctgt gcatttgcac aacagcaagc acaatataaa
    46081 gaaaaccaac gtacagttac ttcttatcat gaagagcaat tagtgacagc tgaccggctg
    46141 cgtcaattag aaaatatcca gctaactgag acattcccag atacagaaga gatcagccaa
    46201 cagttagaag aaataagacg tatgatcgaa ataaagcaag aaaagatata cggtttacaa
    46261 gaacaaaaaa ttagtaatca aaaaatctac gatgaatgtc tttcgattta tcacacaagc
    46321 cagacgaaat tagatgagct tacgcaatta cagcagcttt cgcaaacatt caacggagaa
    46381 aatcctaaga agactagttt ggaacggtat gtgcttcagg tttatcttca agaagtgtta
    46441 caagtagcaa atgatcatct ccaacgttta acgaaaaatc gttatcaatt tgagctagcg
    46501 gataccattg gcagttatcg agggaaaacc ggattagaaa tcaatatcta tgatgatcag
    46561 gcaggtatgt ctcgaagtgc gcataccctg tctggcggtg agagttttat tgcagcactt
    46621 tctttagcat tagctcttgc cgatgtgatc cagacacaag ctggtggtgt agcaatcgat
    46681 gcattgttta ttgatgaagg atttgggtca ttagatgaag aagctttgga aatggcaatg
    46741 gaagctttag aaacgatcga aagtgaagga agaatgatcg gcatcattag tcatgtaaga
    46801 gaattgaaag aacgagtatt acaacaaata agaattgata cagaaggtag cggacaaagc
    46861 aaaatacgct atcaacttgg ataaaaaggg agattggtaa ctatgaaatg gagtattcca
    46921 gaaaaagtca ttgcacgtgg acgtcaatac ttgcaagaag gccgagtgtt atcggttgtc
    46981 cctgaccaag aagaaaacgt gtggcatgca gaagtcttgg gcagtgaatt gtatatggtt
    47041 gatttggatg gtacagcaaa agagatagat tactgccaat gtccctattg ggaggaacat
    47101 aaatattgta agcatacagt tgctgtggaa ctttatttac ggtcgaaaaa tctatctcgt
    47161 atcatgaaaa aagaccagcc aatgctagaa ccggtaaaag caacaagtga gggagaaatc
    47221 attgcaaaag gtttttctcg tctaaaaaca caaaacaatc ctcaagcggt gcaacctttg
    47281 attattgaat accaagtaga aacgattgag actaatcagt atcacccaga gctttctatt
    47341 ttggcacttt acttgcgtat cggttcattg gcatcaccga aaaaaacata tatcgtaaaa
    47401 aacatctatg attttttaca agcttacaca gaaaaagaaa cttacactgt taataaacag
    47461 taccagtttc gtctagataa acgggcgttt caaaaagaag atcaaaaaat actcacgcat
    47521 cttgcaggaa gtgctcagac gcatcaatta ttaggtacga acggcttaca agtcaaagga
    47581 aaactagata aaagatatct gcttctccca gtagaacaag cccgcttcct gttagagcag
    47641 atgaatcaga ctacacgttt gcatctgcag ataaatgaac aaaacgatca aggaatacaa
    47701 ttttccgatc aaaacaaacc tttatcattc aaagtggaaa aatttggaga agattatttg
    47761 ctgcaagcat tgaatgattt tgattttttc tttatgcatt atcagtgggg agttatccaa
    47821 gggacatttt attctctgac gaaatatcaa caaacggttt atcaaacttg gaaacaattg
    47881 attcgccgat tggagaaacc agaagtagtg ttcaagaaaa aagaattacc gtctttgttt
    47941 aaagagatta tccctctgct gtcagagatc ggcgacgtag ctattgcccc tgcagtagaa
    48001 gaggaagtag cagatatccc cgtcgaattt attttctttt tccgaaaagc aaaaggaaaa
    48061 atccaagctc gagtcgattt cgtctatggc gaagtcatct attcgactga cccgaagcat
    48121 gaggtctcga cagaacaggc ttttcaggtg ttacgtgaca cagtagaaga gaaacgagta
    48181 cttgatctat tcagaatgta tcgctaccag aaaaatgaaa ctggttatga acgtccattg
    48241 ccaagcgggg aagaactata tgcctttttc cgcacagaac tatctgtctt ccgtcagttt
    48301 ggtgaagtca gacttggaag aaaattgcga gagttgtatt tagatgcgca taaacatcaa
    48361 ccgaaaatcg aagtaaccga gaccggttca tggctcgata ttcgattcga tgtgacaggg
    48421 atagaggagc aagaaatcga tcatgtcctg caaagcttgc tgagaaatga tgctttttat
    48481 acattagaaa atggtcaggt tctttctttt gattcagaag aatttcaaca aacaagccaa
    48541 gtgttgcaac aattacggga atcgatacgt acagaagaag gaacgatcca tgtacctaaa
    48601 aatcaaggat tgatcattca aaatcaattg gaaaaaagca atgctacttt tagtgaatcg
    48661 ttccaaacga tggtacaaga tctcattcat ccagaaagat accaggcaca gcttcccaaa
    48721 ggattgaatg caacgatgag agattatcaa aagcaaggtt ttcgatggtt gaagatgtta
    48781 ggtcattatc agtttggcgg tattttagcc gatgaaatgg gacttgggaa aacactacag
    48841 acaattgcct ttttactttc ggaaaaagaa gaacggaaaa gcttctctgc tttgatcgta
    48901 gcgcctgcga gcttaattta caactggcag gcagaggtga gaaaattcgc cccttcttta
    48961 agtatccaag taatcaatgg gaataaaaaa gaacgtgaag aattactggc taaagacaca
    49021 gatatccgag tgacttctta tgccagctta agacaggatc tagctaatta tcagtcgcaa
    49081 aaaattgatt atttgattct tgatgaggca caaatggtca aaaatagcag tacgaaaact
    49141 gcacaagcat tgagagaatt agctgtacct cagcggtttg cactgagtgg gacaccaatc
    49201 gagaataatt tagaagaatt gtggtcattg tttgcaacga ttatgccagg atttttcccg
    49261 actaaaacaa aattcaggga acttgccgca gaagaaattg cgcaaatgat tcgaccgttt
    49321 attttacggc gagataaaca aactgtgttg aaagacttgc cggaaaaaac agagatgaat
    49381 ctatattctg cacttacaga agaacaaaaa actgtttatt tagcttactt gcgtcaaatg
    49441 cgtgaagaaa tcacatcaat ggattctgaa gcattcaaga aaaaccggat cgggatactt
    49501 gctggtctga caagactgcg acaaatttgc tgcgatcctc gtctgtttat tgaagattac
    49561 caaggcggtt ccggcaagct cgaacaagta aaagatctac tggtagctgc caaagagaac
    49621 aaacgaagag ttttgctgtt ttctcaattt acagggatgc tgactatctt gcaagaagag
    49681 ttagccgagc tagggatctc gacattttat ttaagaggca gtacgaaacc acaagatcgg
    49741 ttaagtatgg tagacgcatt taatgctggc gaaaaagatg tttttctgat ttccttaaag
    49801 gctgggggaa ctggattgaa tctcacggga gcagatacgg tcatcttgta tgacctttgg
    49861 tggaatcctg ccattgaaga acaagctgct ggacgtgcac atcggatcgg acaaaaaaat
    49921 gtggtagaag tgtggcgtat gatctctgaa ggaacgattg aggaacgcat ggattcactc
    49981 cagcaagaaa aacgcgaact gttccaaaaa gtgatccaag gtaatgaaga acagttgacg
    50041 aaactgactg aagaagacat ccgcatgatt ttaagtgtag gagaaatcga ggagtaagag
    50101 actcgaaaaa gcgttcagta atgggtaaga aagcctttct aaagaagcga ttgtttattt
    50161 gatttaaaaa atagcaagga ggctgggatt ccattttatc ccagcttcct tgctatcatt
    50221 tttttatttc tatttttaaa ggttatacaa agattgtctt cattgtctct aacagctgat
    50281 caaaagaaga aatctcgata tcgcaaattg gagtctgttc aattttgcgt tctcgatggt
    50341 tgaaccaaag cgcttgccag ttagcacttt ttgcacctag aacatcattg tcgtaattat
    50401 cgccaacgta taaggtctct tctggaagca tatgaaattc tttctcagct agctgaaaaa
    50461 tctctttttc tggtttttgg aaacctgttg cttgagaaat gagcatattg cgggaaggaa
    50521 tccaattgtt caattgaagt tgcttcagtt tttttgtctg atggtcagtc ggcccatttg
    50581 tgataattcc taatgggaca ttcttttcct tcaagaaatc gagggttttt ttgacctcct
    50641 catgcaagca gatattatca agttcttcct cgtaaatttt ttgaaataag aggccatctt
    50701 cttccgtaat atgagggtag tctaaatctt ttagtgattg gctgatccga tgtgctcgca
    50761 tatattctag tgtccaatct cctgccatta cttttgggaa gttttcatca ctatgatggc
    50821 ggaaccggat atatagtgga tgcatatctg catctgaaac aagcggaacg actcttttga
    50881 ccgcgtttcg aaatggctgc tgctgatcat acatcgtatc atcaacatca aacacaactg
    50941 atttaatcaa aattttacac gcctttcggt tttcattgat tttttcacaa atccttgact
    51001 attgtaacag aaaaaattgt ctaaaaaagc tgataaataa aggtttttct tactatattt
    51061 ctatttcctt gacagaataa ggtttttata tttcagtttt tttcactaaa aatcgtgaaa
    51121 cacgtcaata agtttgatga acaaattaat cattatggta gaagtcgatt gatttatttt
    51181 aatgcaatct ctgt
//
LOCUS       DACDPS010000011        43590 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.011, whole genome shotgun sequence.
ACCESSION   DACDPS010000011 DACDPS010000000
VERSION     DACDPS010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.011"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            160..840
                     /locus_tag="IWJ07_000727"
     CDS             160..840
                     /locus_tag="IWJ07_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP9332326.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            930..1964
                     /locus_tag="IWJ07_000728"
     CDS             930..1964
                     /locus_tag="IWJ07_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP9332327.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            1978..2313
                     /locus_tag="IWJ07_000729"
     CDS             1978..2313
                     /locus_tag="IWJ07_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332328.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            2297..4777
                     /locus_tag="IWJ07_000730"
     CDS             2297..4777
                     /locus_tag="IWJ07_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332329.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            4833..6392
                     /locus_tag="IWJ07_000731"
     CDS             4833..6392
                     /locus_tag="IWJ07_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9332330.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            6389..7753
                     /locus_tag="IWJ07_000732"
     CDS             6389..7753
                     /locus_tag="IWJ07_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9332331.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            7924..8994
                     /locus_tag="IWJ07_000733"
     CDS             7924..8994
                     /locus_tag="IWJ07_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9332332.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            9009..9677
                     /locus_tag="IWJ07_000734"
     CDS             9009..9677
                     /locus_tag="IWJ07_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9332333.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            9697..10275
                     /locus_tag="IWJ07_000735"
     CDS             9697..10275
                     /locus_tag="IWJ07_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9332334.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            10676..11587
                     /locus_tag="IWJ07_000736"
     CDS             10676..11587
                     /locus_tag="IWJ07_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9332335.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(11965..12882)
                     /locus_tag="IWJ07_000737"
     CDS             complement(11965..12882)
                     /locus_tag="IWJ07_000737"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAP9332336.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            13084..13521
                     /gene="zapA"
                     /locus_tag="IWJ07_000738"
     CDS             13084..13521
                     /gene="zapA"
                     /locus_tag="IWJ07_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAP9332337.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            13579..14127
                     /locus_tag="IWJ07_000739"
     CDS             13579..14127
                     /locus_tag="IWJ07_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAP9332338.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            14242..16602
                     /locus_tag="IWJ07_000740"
     CDS             14242..16602
                     /locus_tag="IWJ07_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP9332339.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            16689..17003
                     /gene="trxA"
                     /locus_tag="IWJ07_000741"
     CDS             16689..17003
                     /gene="trxA"
                     /locus_tag="IWJ07_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAP9332340.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            17185..18963
                     /gene="uvrC"
                     /locus_tag="IWJ07_000742"
     CDS             17185..18963
                     /gene="uvrC"
                     /locus_tag="IWJ07_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAP9332341.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            19108..20007
                     /locus_tag="IWJ07_000743"
     CDS             19108..20007
                     /locus_tag="IWJ07_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9332342.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            20000..21262
                     /locus_tag="IWJ07_000744"
     CDS             20000..21262
                     /locus_tag="IWJ07_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9332343.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            21423..23321
                     /locus_tag="IWJ07_000745"
     CDS             21423..23321
                     /locus_tag="IWJ07_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAP9332344.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(23651..24370)
                     /locus_tag="IWJ07_000746"
     CDS             complement(23651..24370)
                     /locus_tag="IWJ07_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAP9332345.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            24707..25039
                     /locus_tag="IWJ07_000747"
     CDS             24707..25039
                     /locus_tag="IWJ07_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332346.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            25026..27029
                     /locus_tag="IWJ07_000748"
     CDS             25026..27029
                     /locus_tag="IWJ07_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAP9332347.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            27048..27518
                     /locus_tag="IWJ07_000749"
     CDS             27048..27518
                     /locus_tag="IWJ07_000749"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAP9332348.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            27539..28123
                     /locus_tag="IWJ07_000750"
     CDS             27539..28123
                     /locus_tag="IWJ07_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAP9332349.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            28138..29124
                     /locus_tag="IWJ07_000751"
     CDS             28138..29124
                     /locus_tag="IWJ07_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAP9332350.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            29114..29425
                     /locus_tag="IWJ07_000752"
     CDS             29114..29425
                     /locus_tag="IWJ07_000752"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAP9332351.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            29447..31228
                     /locus_tag="IWJ07_000753"
     CDS             29447..31228
                     /locus_tag="IWJ07_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAP9332352.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            31221..32597
                     /locus_tag="IWJ07_000754"
     CDS             31221..32597
                     /locus_tag="IWJ07_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAP9332353.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            32604..33239
                     /locus_tag="IWJ07_000755"
     CDS             32604..33239
                     /locus_tag="IWJ07_000755"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAP9332354.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            33269..34624
                     /locus_tag="IWJ07_000756"
     CDS             33269..34624
                     /locus_tag="IWJ07_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAP9332355.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(34816..36210)
                     /locus_tag="IWJ07_000757"
     CDS             complement(34816..36210)
                     /locus_tag="IWJ07_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP9332356.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRSNQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            36302..37273
                     /locus_tag="IWJ07_000758"
     CDS             36302..37273
                     /locus_tag="IWJ07_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAP9332357.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            37270..38052
                     /locus_tag="IWJ07_000759"
     CDS             37270..38052
                     /locus_tag="IWJ07_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAP9332358.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(38221..38592)
                     /locus_tag="IWJ07_000760"
     CDS             complement(38221..38592)
                     /locus_tag="IWJ07_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP9332359.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            38726..39169
                     /locus_tag="IWJ07_000761"
     CDS             38726..39169
                     /locus_tag="IWJ07_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP9332360.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(39212..40021)
                     /gene="yidA"
                     /locus_tag="IWJ07_000762"
     CDS             complement(39212..40021)
                     /gene="yidA"
                     /locus_tag="IWJ07_000762"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAP9332361.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(40040..41407)
                     /locus_tag="IWJ07_000763"
     CDS             complement(40040..41407)
                     /locus_tag="IWJ07_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9332362.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(41391..42221)
                     /locus_tag="IWJ07_000764"
     CDS             complement(41391..42221)
                     /locus_tag="IWJ07_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAP9332363.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            42384..42821
                     /locus_tag="IWJ07_000765"
     CDS             42384..42821
                     /locus_tag="IWJ07_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAP9332364.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            42897..43517
                     /locus_tag="IWJ07_000766"
     CDS             42897..43517
                     /locus_tag="IWJ07_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAP9332365.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
BASE COUNT        14279 a         7487 c         9251 g        12573 t
ORIGIN      
        1 ttaataaaaa aggaagaata ttcatgtaaa cgattttatt gtttttttct cttttataca
       61 aaaagggtca taatttcaag taagcggatg aattcatgag gattttttgc atactgtctc
      121 tactatactt aggtcactaa gaaaaaggag tgatttatta tgaaaaagaa catgattggg
      181 acattattac tttcaacact tcttctaggc ggcttggcaa caccagcttt tgctgagggt
      241 caagcgactt caaaaggaga tattaccttt actgaaccaa caaataccgt ggagcctctt
      301 aaccctactg atccttcaaa accagtagag ccagcagatc cggaaaatcc agcaactggc
      361 cagacaggtc ctttaacact ggatgtagtt cccgaattgc catttggtac acacgagatt
      421 gaaagcggaa caaaaacgta tcaagtagac gcttcaaaaa acgatacacc ttatctccaa
      481 gtatcagatc gtcgaggtgt aggtgcagat ggccaagcac aagggtggaa cgtgacagtt
      541 tcggtttcag attttgtaaa cggttcacaa gttttgcaag gcgctgaatt agattttgga
      601 acatccactg ttaaaagtac gtcagataac gaatccactg gaccaactag tcaaactgta
      661 actgggttga gtaaagcaag tgcagcgaca ccaatattta cagcagcaaa agatcaaggt
      721 ctgggaacat ggctttctgt ttatgatcca gcaaatatca cactgaaagt gccgaaagca
      781 gcagctggaa cattcacggc tgatttgact tggaacctcg tggctggccc agtagcatag
      841 agtatgaaat aaattcaaca aactgcggtg aaaatactat aggtgaagga ttttttaccg
      901 cagttgttgt tgaaaggatg tggtggacaa tgaatcaaag taaacgactg tttgtttcgt
      961 ttttttctat tttaagtcta tttttcattt ttccgtcaat cagccaagca aaggattcag
     1021 caggagactt tggaatcaaa ccggtatttc cagagaacca aatagataaa gcaatcggtt
     1081 attttgattt attagtggca ccggaacaaa atcagacact agaagtaatt atcagcaatt
     1141 cttcagatga agaacgaaca tttgaggtct ctgtcaatcc ggcggtaacg agtgatggtg
     1201 gaacgatcga ttatagtcaa aaaaacccaa cgttagatga gacattgccg tttgatgtcc
     1261 gtgacgtttt gctcattgcg aaaaaggaaa tcaatgtatc agcacatgca gaaacaactg
     1321 tacctataga agtgaaaatc ccagcaaaaa gctttaaggg acgtgtatta gcgggtattc
     1381 atgtttcacc taaagaagaa gctgaaacag aaaatgctac agaaggagca caaatcaaaa
     1441 atcgtattgc ttataatctc gcagtcgttt tacaagaaag tcaagagacg atagaaccgg
     1501 atctgaaact tttatcagga gatttggacg aggtcaacgc aaagccaact gttcagcttc
     1561 gtttccaaaa tcctcagcct aggattatca gcaatttgat atttacgagc aagatctttt
     1621 atgaaaatca gctttatatt gaaaatacgt ctaatgcatt tttagttgca ccaaattcga
     1681 attttcactt gaatcttgat ttagctggtg ataaagcaaa agccggcgat taccgcgcag
     1741 aaatcatagc aaaatcaggc gacagtaatg aatggcgatt tacacaaaac tttacgatca
     1801 aaaaagaaaa agcacaaaaa gtaaatgaaa attccgtttt tgcagtacaa gaacaatctt
     1861 tcccatggct ttatgcagga ttggttgctg cagttctagt gttatggttg attttgctgt
     1921 ttttgtgggt ccacaagaaa aagaaaaaag gagaaacaac atgatgaggt gatttcgttg
     1981 aaaacatcga tcttattggg agggattatt gcaagtttgc tgttcttcag ctggcaagag
     2041 ccagtccaag cagataccaa aagttcaggt acgattacat tttatgatct aagtccaatt
     2101 ccgcatccaa gtggttcagt caatggcgca tcaaaacagc agacacagct accgatttat
     2161 aaagaatcta atcaacatgg cgtattcccg gagacgaatg aacaaaagat ccgttttctt
     2221 tatttgatca ttggtataga attatggctt cttgcgtggt tgttgttatg gagaagaaag
     2281 gaaaaggagg atgaacatgt tgctacaaaa taaattttca ttttttcgct ccttcttttt
     2341 tacttcgctt tttgttttaa gtatatttat cttttcacct tcggcagaag ctgttggagc
     2401 aacgcctcct gccatttctt atgatccata cacgtatgaa aatatcgtac ctccaaatgg
     2461 tggacgtagt gcagtcaatg gcgggaagct tgtccaacta actggggcta accaattgcc
     2521 taaattgact gctacttatt tgaacgatgg ttcaaatgat ccttggaaag gtggcacggt
     2581 ccgttttcat tgggaaagcc atgtgaaact aacaaatgta cttgatttgg tgaatatcac
     2641 tgcacaagga tttgcatttg tggcgatcat caatttgccg gaacatgtat caagcagtga
     2701 tgtattaaaa gcaattgact ggaacaacgc ctacttgtat attgccggtg cccccatcaa
     2761 ggttactagc ggaagtttat tatcagcagg aagccatacc ttgcgttttg ggatggggtc
     2821 gtgggataca aatactctat tgacagatat tattgctatt attacgcgag gcgaattcaa
     2881 cgtggacgat atcccgcttc aatttgatgt tacagctaat gtcgcgaaga tgacagaaaa
     2941 cggagataaa tatgatacga gtccaaacaa aattttgacc aagggtaaat tacaaccttc
     3001 tatgtctaaa aaggccgttt tttctgttga tttttatgat tcgaataata ttatccaaga
     3061 tcgtatgttc aattcgattt tagctccagc tgcaggtaga ttttatccta aattaactgg
     3121 cggaaacttg aatccaggag catatgcatt ttctgcaagt gcagagataa atacgtggaa
     3181 tagttacatc tcaccaagtg attcaacagg aaaatataat acatttgctg ataccacaga
     3241 atacgtggat ggaaataacc agaaccttcc aggctctacc aatagtcgga cattgacgat
     3301 ggggctgact gaaggacgtt ttgatcagtt gccatttagt cgctttgatc gcgtagtcaa
     3361 ctattttaca ggaaaaaacg ttacacaagg agcaatactg actcatactc cagcaagcac
     3421 aagtggattg aatcagacag ccaatatcgt gtacagtggg aaagatgcgc aaggcggatt
     3481 gctttctcct gttgctttaa aggtattgga gcagtacaag ctagacggaa cggttttacc
     3541 aacaaactta acagccgata gtcagtgggg aaagctcctt tcttctgcat cgtaccaagt
     3601 cacggccaat tggtcagcaa ctgaactaaa aaagggttcg atccattaca ggctttattc
     3661 gaaaggaacc caaaaattag cgacaggatg gacagatcaa cttttccaaa cgatccaatc
     3721 aaataacggg ggtgtaaata cagctacttc tacactacct gcattggctt cagggcaata
     3781 ttattttgat tatcgcttgg tggacgatgt acttactaac atgtatccgt cttttgcgta
     3841 caaatggcag tctgaacaag ctggaatcac cactcttcct caaataacag tggctaattt
     3901 tccatctgtg agtgcagata gtcagctaaa aaaccttagc agacaacctg agacaggaga
     3961 tcccctgtct gctctatcag gagatacgat tcaagaaaat gtgacattca cgttaacaaa
     4021 atctggggat gcgataaccg acaaaaaaat cacgatctca cttcctgaaa atactactta
     4081 tatcaatggt tccttaaaat taaatggtac aactatcgca gataccggca ttcaacaagg
     4141 agtttctgtc ccagcagatc tattgagcaa aataggagat accattcatc ttacttacaa
     4201 ctatcaatta aatactgtag atacttcttt acaatccgtc agcatactga caaaggcagc
     4261 tgtactttcc agcaatatta cattagcaga tggtgctaag cttcctaacc cagttgtcca
     4321 aacatctgct aaaacaattc ttgtccccaa acaagaatta acattagtta atgtaccaga
     4381 tgattttaca tttggtaacg atctccccaa accgttgaag acaagttatt atgaatcaaa
     4441 aggcgatttt tcttttgatg tgcgggatac tcggcttcca agcacgtctc cttggcaact
     4501 gacaggtaca ttgacttctt tatttaagaa tgatcaaggt caagaattaa gcggaacaaa
     4561 attatacttc aatcattctg gatcaaagca attgattcag caaggtcaaa acacgttgat
     4621 ttatgagagt gatggaacag ctaaaggaga ggttctcgtt gactttcccg ataccgatgg
     4681 tttactctta gaggtaaata gcagtacgaa tgctcaacct ggcgcaacgt atcaaggcat
     4741 ggtcacatgg gaactgacag ctgggccaac cagctaagtg attagaattt gtatggatct
     4801 gtgataactg tcacagatcc tctttttcaa ggatgagaag gaaacttctg ctgttaaaat
     4861 cagaaatgct tgaaggagtg gacttggaac ttttcagttt gcttgaaaaa aatgaacaac
     4921 ggcaaatcaa acttttccaa cttttattag aggaaaaagg agaggtttcc gttaataacg
     4981 tacttacccg cttatcagtt gatcgggcta ccttgaaaga agatctgttt tatttacaag
     5041 ctaacttgga aaatatcaaa caattggaaa ttgttatcca agaagggaat attttattaa
     5101 tcagacatgg aaatatagca gcagtcgatg tttattatca ttgttacttg cgctattcta
     5161 caaaatatca gatacttctt tatctgttgg aaaatggctc atttgatcgg cagaaacttg
     5221 tcatggaact taatatcagt tcggctactt tgtctcgcag agtgaaggaa ttaaatgatt
     5281 ctttaaatga gtttcatctg aaaataaaaa gcgggcggct gaccggtcca gaaagtcaaa
     5341 ttcgttattt ttatttccaa ttattttggt ttggacgtcc ctatcttgaa aacaaaaaag
     5401 aatacagtga tttatcagga aacacttttt tagatctgct gcaaaaagag tttagttttg
     5461 ctattacaga agaaggacga ttgaaatttg aaatatggct ttccattatg aagaagcgaa
     5521 tgcgctgtac gaaaaaatcc aaaccagatt atagcaatct tcctgttgga ttgtttcaaa
     5581 cgacacaatt tttattggac ctccatacat ttttgacccg ttacttcttt caatatgcct
     5641 ttccttggaa tcaaaatgaa acgttggttt tttatctttt catgatcagt aactttacat
     5701 tagatacaca taactcgcat gtccaggagt ttttgataag cgaaaaatac aaagacgacg
     5761 tagtagctca attgaatcga acgtttaaac aatcgataga agaatatttc tctttttatg
     5821 tttttccaga agagatttcg caaaagattg cgttgacagt gatgcaaaat cacgctcatt
     5881 ttgcctattt tagaggatgg atgaatattt ttggccaaga aggattaaaa cagcggttaa
     5941 ctgaagtaga agataaacaa ttcctggttt tatgtgaaag attggtcaga caaacatttg
     6001 ctattgttga ttttactgaa gtagacgagg aaaatgcccg ctttgaatta tttgctcgct
     6061 atgcatctat tttacggacg ctgatcaagc agttagaatt acgtctgcaa attgcttatg
     6121 atcttcctta tgaaaggcta atggcggata tcgtgaaaga aatcatccag gaacaattgc
     6181 tgaatacgat caaatatgat ttagtcgaat acgagaaaga aaagcagtat gatatcattt
     6241 tgactagcca attgaaagag tacccgtcac agaagtccgc taaagttttt gtattcacta
     6301 gcaacgaata taattatgat ttcccctatt tgaaccaatt tttaaaagaa tgttatttag
     6361 aaaaattgaa tttaaggatg aataaaacat gaaaatcgtg atcattggtg cttcccatga
     6421 aggcatcaca gctgccctaa atctaagaaa acttcaaccg aaagcagaag ttttactgat
     6481 cgatgaagat cataaagatg gactaggcta tgtttcaaat ggaatcaacc tttatttaaa
     6541 aggaaagata cgtagtttag ctgaagttgc ccataacatg cggactcttc aagaaagtgg
     6601 ggcaaaactg atcaccgaat ggagagtaac tgaacttgat ccagacaaac atcagctcat
     6661 cctcgcatcc aaagaaggaa aaaacgagac cataacctat gataaattaa tcgtagcgac
     6721 aggttcctct cctgtcacct tgtataaaca aattgaagct gaaaatgtct acacctataa
     6781 aaatctagtg caatctaagc aagtacttgc cgctttaaaa gaagcaaaag aagtagtgat
     6841 cttcggtgct ggttacattg gattggaatt agcagatgct ttgagaaata aaggatacat
     6901 tattcatcta gttgattata tgccgaatgt tttatcaaga tatttcgata aggatatgat
     6961 caacagtttt caaaatcagc tgcaaactaa gcaaataaat ttttatccaa atgaattctt
     7021 gatcgattgg aaaaaatcag aagagaaagt agtatccgta caacttctgt ctcaaacaat
     7081 aaaggcagat atggtcattt tttctgccca aactcgtcct aatacgacat tgttaaaaga
     7141 aaaagtggct ttgtatgagg atgagactgt gatagtcaat gaatatctgc agacaagtga
     7201 tccggacatc tatgcgattg gagatatcgt cccagtcagt tttgacaaaa ataaacggca
     7261 tctcttttta cctttagtta ctcgagctgt acatatggca agagcagtag cgctaacttt
     7321 gagtggtcag ccaaccgctt acgacctcag acaaaagata acagcagcag tcattataga
     7381 ctacttttta ggaactgtag ggctgacaga agatgaagcg ccattcttgg aacaaaatac
     7441 cggttcgtgt tcaggagaat ttgatctatt tccacaatac gacgaagaaa acaaaacagt
     7501 aaatgcaaag ctgatctacc atccggatac attagaaata attggcggac agctgataag
     7561 ccaagaattc ttattatcag atttgaacct gcttgcggat atcgtgaaac acaaaactac
     7621 gattccacag ctagcagttg aggattttgg atttctgtct gaatacacct ctcgttttca
     7681 ctacctcaat gagttagctt ttaaagtact agctaagaga gcaaatagca atagagtaga
     7741 taaaagactg tgagaaatat cacggtcttt tattgtgtgg ggatataata aatattaatc
     7801 aatcgagaat aaaaaattca tataatgaaa tagcggaaga attttttgag cataatattc
     7861 gccaaaaatt tatcaagtga taaaattgtt gtataaaatg attatttcag gaggaggagt
     7921 taaatgtttt tagcattaaa tgaaattatg cattcaaagt tacgttatgc gttagttgca
     7981 ggggtgatgt ttttgatcgc ctacctggta ttttttttga ctgggttagc ttatggctta
     8041 gctcaagata atcgaacagc agtcgacaag tgggaggcag attcgattgt gctttcgaaa
     8101 gatgcaaatt caaatctagg gatgtccatg attactaaga agatagcaga agaagtcgaa
     8161 ggtgggaaag tggcgtatct tgcccaaaca cctggagtcg tgactagtaa agacagtaca
     8221 gaagaaggaa aaatcaatgt tagcttcttc ggtatcgata aaaatcaatt catcatgccc
     8281 aatcttgttg aaggaaaagc ttttgataac gatgatgagg cagtaggcga tattagtttg
     8341 aaagaagaat atggtctagc cataggagat acagttaagt tatctggtag tgacaagacg
     8401 tttaaactaa ctggctttac ggatcatgcc aaattcaatg tctcaccagt tctctataca
     8461 accatcaatg cttaccaaga aatccgcttc gaaaaagaag atacaagtga aaatgcacgg
     8521 attaacgcaa tagttgtcag aggtaaaatc agtaatttac ctgaagattt agaacagatc
     8581 aaaatttcta aattcatcaa tgaattacct ggatataatg cgcaagtatt gacttttgga
     8641 tttatgatcg gatttttgat cgttattgcg gctattgtga tcgggatttt catttatgta
     8701 ctgacgatgc aaaagattaa tatctttggt gtaatgaaag cacaagggat cacaggagga
     8761 tttattgcgc gttctgtagt ggcacaaaca ttcattttat catttgttgg tattcttctt
     8821 ggtctattag gaacagtggg aacatccctt gttctacctg atgcggttcc attccaaagc
     8881 aattggctgt tttttggcgt cattagcttg ttgatgttag tagtagctgt gttaggcgca
     8941 ctattttctg ttcggacaat cgtaaaaatc gatccgttga aggcaattgg ataagaagga
     9001 ggaagaacat gaaagcaata gaatttaaag aagtagaaaa aagtttccaa gatggcgacc
     9061 aagtagttaa agcattgaaa gaaacaacca tgagtatcga taaaggggaa tttgttgcaa
     9121 tcatcggtcc ttctggttca ggtaaaagta cttttttgac tttggcaggc ggattgcaga
     9181 cgcctagcag tggctctgta tttatcaatg atcaagaatt cagtaatcaa aaagaaagcg
     9241 aacgcgttaa actacgtttt aaagaaattg gctttatttt gcaagcttcc aatctggttc
     9301 cttttctgac agtaaaaaag cagttattat tagtagataa gattaaacat gaacatcgtc
     9361 aaaaagaagc aaacgaatta tttgagcagc ttggcgtgga taagttgatc aacaagtatc
     9421 cagaagaact atctggtgga gaacggcaaa gggtcgcaat cgctcgtgca ttgtacaatg
     9481 atccgtccat tattctagca gatgaaccaa ctgctagttt ggattcagaa aaggcacgag
     9541 aagtagtcga tatcttggca aaagaatcaa aagacaaaaa taaagcaacg atcatggtta
     9601 ctcacgatac acggctgatt gacaaatgcg atagaatatt tatgatggaa gatggtgtat
     9661 tgaaagaaaa agaataacca aaaagcaggt ggaaaaatgg tccatcgata caacaaagat
     9721 acacagcaaa aaattttgaa agaagcgact gacttgttta tgtccaaggg atatctcggc
     9781 acttcaacaa gagaaatcgc acaaaaagtt gggatcaccc agcccaatct ctatcattat
     9841 ttcggtgata aagaaaaact atatacggca gttttagaaa accacttaaa agatgttgga
     9901 aaagctttga gagaaatcgt tcaaacaagc gaaacgggat ttcaagtaac cttgacaaaa
     9961 atggcgcagt ttttgattga gacacatctg gtcgatttgt ttctgatgct tcacgatctt
    10021 gaaagcaatc tttcaaagga aacaagagat catcttttct ttctatggaa gaaaaattat
    10081 cgtgaacctt ttgaagaaat cttttcagca aaccaatctg tgctaagaaa tgggatttca
    10141 aaagaaatcg cagcaaggca ttttttctta gtgttagcac cttacatcac tcggtcgaaa
    10201 agacctgtgg aagaaccgct aaatgtggcg caattgatcg atttatattt gaatggtgta
    10261 ttaggagaag cgtaaagctg ttatataggg agaaaatagt taggtgaaga agcaagatat
    10321 gacgaagatc gttatatctt gcttctttat ataggtagtt tttcataaat acaaatttgt
    10381 tattctctca tgattatcag ggtatatttt tatttattat gtcggtgttc caggaagtgt
    10441 tttaatttaa taacgaagaa attaattgtt tgtagaatga aggaaactat ttattgggta
    10501 gatgaatcac cacgaatagt caaaaggaga tttcaggata tgccaaaaat gaaaaaaatc
    10561 attttagcag taatgttatt ttctacggtt atatctttta cagccccttt tttgcttact
    10621 taagctgatt ttaagttttg gcccttagtc tggatctacg aggtgagagt caagaatgaa
    10681 aaaaataatt ggagcttttg tatttgcaat cattatcatt attatctgtt ttcaaataga
    10741 tgtttttggt caaaaaaaga tagttggaca aacggtcagc agtacgaaca gtacggttac
    10801 aaagacaaag gaatcatttg atgatacaga acaagagaca ttaatcaaaa tcggaaaaaa
    10861 tgaccgtaca ctatatacag atgactcgct aactacccca cttgcaacta taaacggcgg
    10921 agagctggcg gagtttcttt ctgaaacaaa aaatgcttat cagatcaaga caaatgatgg
    10981 ctatactgga tatttagccc tcgaagatgg aaccaaagtc acaaagaata tacaaacaaa
    11041 accggaagag ctatctgatg cagtgattgt attagatcct gggcatggcg gaaatgatac
    11101 tggggcatta agcaatgatg aacaaacaga agaaaaagat ataacattaa gtacagccat
    11161 aaaagtaaaa aaggcgctag aggatgctgg agcaacggtt tatttgaccc atactacaga
    11221 tgaattagta cagcttggag atatatgcga ctacagtgaa gaaaaaaagg cagatgtctt
    11281 tatcagttta cacgcagact ctacggaata tgcgaatgaa gcaactggaa tcacgacata
    11341 ttattactat ggacaagagg aaacattagc acagacaatt gctgactcct tcactgatct
    11401 gcctctggat tcaagaggaa tctccactgg gaactatcaa gttcttcgag aaaatcttca
    11461 accttctatt ttaatcgaga tgggttatat gaataatgat agtgatttaa aggaattagt
    11521 aacagacagt tatcagcagc agattgctgc aagtttgaca caagcattga ccacttattt
    11581 tcagtaataa ataagcccag ccaaacaaaa atagctcctc atatttttcg tttggccggg
    11641 cttattatcg cagatgcaag cttttaaaca ccgtttaagg aagaggtcgt gagcctgccg
    11701 ttcaactctg agtcacaagg agcaatttca aaaaacagct tctcatgttt gatgactatt
    11761 gtgacttgtg accgaggagt tggcatgaga acaccgttta agaaaaaggt tgtgaaatca
    11821 acgttttgtt ctgagtatta gagaaacaaa cggaaaaata gttcctcata ttttgccgtt
    11881 tgttgagcta ataccgagga tcagatgatt gaacaccgtt tattcggaac aagggactgt
    11941 gacatgactt ttgtcacagt tccattattt ttttagtagc ttcaaagctt tttctgtatt
    12001 ggcaaaatga agtttggcat atttttcaag cataggcatg ccgcctttct ccaatagttt
    12061 tgccgctact tggtcagatt tcgttccagc tcctgacgga agggtgattc cagcttcagc
    12121 tgatgctttg tctaactctt ccaaaaagct ggcacgactg atgatagaag cagcagcaac
    12181 agctaaatga tattgttctc ctttagtaac gaagtacagt ttttcttgta attgattttt
    12241 ttctaaacgc gcatattttt taaaatttgc ttccggcgta aactgatcga tcaaaatcgc
    12301 ttctggtttt tctggctcga ttttttttag cagcaaatgg atcgcttgat tgtgcaaagc
    12361 gactttcatc cgaacggcat tgtattttgg ttggatctca ttgtattttt ctggagataa
    12421 gacaagcagt ttatagggga tggttttttt taagatgtca gcaagctgga tgatttggct
    12481 atcttttaac tcttttgaat ctctaacacc aagtttacgt aattctgcaa gctgatcacg
    12541 tttcacataa gcagcacata ctgttagcgg accgaaatag ctgccatttc cgacctcatc
    12601 actacctaaa acggataatt gactgatatt ttttggcaaa gaagaggctg tttttgctgg
    12661 cttggcttgt ttatcctgtg cctcccccca tttatcagct tctattgctg cattcgtacc
    12721 ttgaaacatc acttttccgg atgtataagc ggtaatggtc gtcccgtctt tttttgcagc
    12781 aaagctagta taaggaatct gtttatttaa caaataagag cgatagtatg ctttcatttg
    12841 actaagttgg tcgtttgttg ctttgataat gacgttgctc ataaaattct cctcgttttt
    12901 atacataaaa ctataccgca actcacgact tttcgttttc taagagattg cgagacatag
    12961 cattttttta aagtttatca tataatagtt aagtatgtga aaaatatttg tggagaacgg
    13021 tgaaagaacc aacttcatgg tacactaaat aaaactttca tcaaatagga ggaaatgatt
    13081 cgtatggcgc acgaaaaaac acgctacaaa gcggtgattg ccaaccaaac atatactatt
    13141 atcggacggg aaaccaagca tcatatggat atcgtaacga agctgatcaa tgaacagtta
    13201 gcagaattga aacaattatc accacaaatg gataatgaac aagcagcaat cttgatggct
    13261 gtcaatgcgc tttcggatca attgaagaaa caagaaagaa tattggagct ggaagaagaa
    13321 acagcagaat taaagaaaaa aatgatcaaa tttacagagc ttgaaaatcg tgtgaaacgc
    13381 attgaagcaa tcgagaacga agctcgggaa gtattgaaag aaaatggtca agcagatcat
    13441 accattaaaa atcatgtgga agcacaacag attttaaacg agaaacgaaa aggacaaatt
    13501 aaacaaaaat cttctaatta agatcctttt atcaaaataa gaaaaattta aaaaagttag
    13561 aagagaaagg aacgtagcat gctgagtttg ctgattttat ttttattgct aattgcgttt
    13621 ttttctggag cacgtcgcgg gtttgctttg caaatcatat tcgcgattgg atatgtattg
    13681 tcatttattg ctgcccagca tttttacaaa ccattagcaa gtcatttgga attatacatt
    13741 ccatacccag ctgtcacccc aacatcaaag ttagcatttt ttgatcaggt atttgccttt
    13801 cacctagatg aagcttttta tgcaggaact gcttttctga ttattttgct aattggctgg
    13861 ctgcttaccc gatttgtggg ggtttttgtc catggattga cgtatgtgcc gatcttgaga
    13921 caggtagact ggattgcagg aggcatcctg tcactaatca tggcttacgt tactatattt
    13981 ttgattttgc agttacttgc ctttgttcca ttagatgtgg tccaaaatca attcggcgag
    14041 catagtttcg ctcgttttat cgttgagaga acgccatttc ttaccaacaa gatgtatgag
    14101 ctttgggtaa cgaatatttt gaattaagga aaatagtgct ggaaaatatt tcgttttttg
    14161 tacagagtag tttatttaag aaggctgcgt agcgtatgtc cggcacagct cttttttcat
    14221 agtgaaagat aaggtgataa aatgaataac cgaatccttg aaacattgga atttgaaaaa
    14281 gtcaaacaga tggtcagaca atttgtagtg actgcacaag gaaaagaaga actggcagaa
    14341 ttggtaccag tgagtgagaa acaaacgatc acaaactggc ttcaagaaac tgaggatgga
    14401 ttgaaagtgc aacgtctgcg aggcgggatc cctataccta aaattgaaaa tatccgtccc
    14461 catatgaaac ggattgagat cggagcagac ttgaatggag tggagcttgc acaagtttcc
    14521 agagtattgt ctacgacaag cgaattgaat cgtttcattg atgatctttc tgacagtgaa
    14581 atcgaatttg cacgtttgta tatgtgggca gatcagttag tcacgatccc tgttttgagc
    14641 cgccgtttaa aggaagcaat cgatgaagac ggacgcgtga ctgatgacgc ttctcctgag
    14701 ttaaagagca taagacagaa cattcggcga agtgaacaag ctgttcgtga acagttagat
    14761 gggatcgtcc gtgggaaaaa tgcaaagtat ttaagtgatg cgatcatcac catgcgaaat
    14821 gaccgctacg ttatcccagt caaacaagaa taccgcggtg tatttggcgg tgtagtccac
    14881 gatcaaagtg cttcaggtca gactttattt attgaaccaa aacaagtggt ggatttgaat
    14941 aatcgtttgc gtcaatacca aatcgctgaa cgaaatgaga ttcaacgtat tttaagtgaa
    15001 ctttctgctg aacttgttcc ccatcgtcaa gaaatcatcc acaatactta tgtgattggt
    15061 aaaatggatc tgatgaatgc aaaagcacga tttggtaaag aagtaaaagc catcgtgccg
    15121 ggaatcagtg aagacaatca tgtcgtcttg aaacaagcaa gacatccact gatcgatcag
    15181 gaaaaagtag tttctaatga catcacaatc ggaaaagact atcaagccat cgtcattaca
    15241 ggacctaaca caggtgggaa aacgatcacg ttgaagactt taggtctgtt acaactgatg
    15301 gggcaagcag gattaccgat tcctgcaggt gaagaaagtc aaatcggtat ttttgaggaa
    15361 gtatttgcgg atataggaga cgaacagtcg attgagcaaa gtctatcgac cttttcttct
    15421 catatgacga acactgtaga tattttatca aaagtcaatg aaaaaagtct ggtattattt
    15481 gacgagctcg gtgcaggaac agatccgcaa gaaggtgctg ctctagcaat cgcaatctta
    15541 gatgatcttg gtaaaaagtc tgcttatgtg atggcaacaa cgcattatcc tgaattgaaa
    15601 gtttatggat ataatcgggc aaatacgatt aatgccagca tggagtttga tgtggataca
    15661 ttaagtccaa cgtatcgttt gctgattggc gttcctggac gaagcaatgc gtttgaaatc
    15721 tctagccgat taggtcttga tacagaagtt attgatgaag cgaagcagtt gatgaatgat
    15781 gagagccaag atttaaacga aatgatcaca gatttagaga atcgccggaa aatggcagag
    15841 accgaatatc tggaaatgcg tcattttgtt tctgaggcac aagaacttca cgatgatttg
    15901 aaagaagctt acagctattt ctttgaagaa cgtgaaaaag aaatggaaaa agcaaagaaa
    15961 aaagcaaatg aagtcgtgtc agaggcggaa gaaaaagcag aaaaaatcat tgctgatatt
    16021 cgtaaaatgc aacagcagat tgggcaagga aatgtgaaag agcatcagtt gatcgatgca
    16081 aagacccaat tagcaaatct acatcaagaa gaaactttga agaaaaataa agtattgaag
    16141 aaggcgaaag aacaaaaaac gttgaaaccg ggagacgaag tgttggttac gacttacggc
    16201 caaagaggga cgcttttacg taaaaacggg aatcagtggc aagtagaaat cggtatatta
    16261 aaaatgaatg tgtccgaaga cgagctgacg cctgttgctc ctcaaaaaga gccgacacaa
    16321 cgagttatcc atgctgtacg atctgaatct agtagtcacg tgccgaatca attggacctt
    16381 cgaggaaaac gatacgaaga agcgctaagt gaggtagatc aatatttgga ttctgccatt
    16441 ctagctggtt atcctcaagt gactattgtc cacggaaaag gaacaggcgc acttcgcaag
    16501 ggaattactg attatcttaa aaaccatcgg agcgtcaaaa gctttgagtt tgcaccggca
    16561 aaccaaggcg gaaatggtgc gacaattgtt aaatttaagt aaagcttact aaaggtaagc
    16621 agatggttcg ctatccaagt atagaaatgc tatactagtc atagatttta aaaatggagg
    16681 tctttgatat gtcacaagta attacagata agacatttaa tgaagaaaca gataaaggac
    16741 ttgtattgat tgatttttgg gcaacttggt gtggtccatg tcgtatgcaa gcaccgattt
    16801 tggatcagtt ggaacaagaa tatgatgaag aagaattcag aattgctaaa atggatgtgg
    16861 atgaaaatcc agaaactcct caacaattcg gtatcatgag cattccaaca ttaatgttga
    16921 aaaaagatgg tcaagtagta gaaaaagctg ttggtgttca ttcaaaagaa caactacgtc
    16981 aaatgatcga tcaatattta taaaaactta cagcaaaaat aagctgaggg tgggactgaa
    17041 tccagttccg tcctcggttt ttttgcggtt gagttaaggt tattcaaatt aatatttttt
    17101 gagtataatt acgttgtcag gctggattgt ttttttgaca gacatgctat aataacttta
    17161 attgaatatg aaggggagta ccagatgaac gaacgaatca aaaacaaatt agctttactt
    17221 cctgatcagc ccggctgtta cttgatgaaa gataaaaatg gaacgatcat ttatgtcggc
    17281 aaagccaaaa tattaaaaaa tcgtgtccgc tcatatttta ctggtagcca taatacgaaa
    17341 acggaacggc ttgttagtga aatcgaagac tttgaataca tcgttacaga atcgaatatc
    17401 gaagcattgc tcttagagat caatctgata aagaaaaatg atcctaaata caacattatg
    17461 ttaaaggatg ataaaaccta tcctttcctt aagataacga atgaaaaata tccccgtttg
    17521 gtgattactc gtaaggtact aaaagacaaa gcactgtatt ttggaccata tcccgatgtc
    17581 ggtgcggcga atgagacaaa gaagatcctc gatcgattat ttcctctgag aaaatgcaaa
    17641 cctagtcaga cgaaggagcc ttgtctttac tatcatttgg gacaatgcct gtgtccttat
    17701 tatttcgacg tagatccgaa agtctatacg aaaatcgttg atgaagtgaa aaacttttta
    17761 aacggaggac acgaaaaaat cgagtcagag atccatcaga aaatggaaaa agctgctgaa
    17821 aacatggagt ttgaaaaagc tgcagaatac cgagaccaga tccgtgccat tgagacaatc
    17881 atgactcgcc aaaaaatgac aaatactgat ttactggatc gagatgtatt tggttatgcg
    17941 attgataaag gatggatgtg tgtccaagtg tttttcgtaa gacaaggaaa gctaattgaa
    18001 cgagatgttt caatgtttcc tttctataac gaagcagaag aagatttctt gacttacctc
    18061 ggtcagtttt atcaggaaaa tgagcatttc attcctaaag aagtcttgat tcctgatacg
    18121 atcgacaagc ccagtgtaga agctttgtta gctacgaaag tgctgcaacc aaaacgagga
    18181 gaaaagaaaa aattagttga attggcgagt aagaatgcct cagttgcttt gaatgaacgg
    18241 tttgatctga ttgccagaaa acaggaacgg acgatcggag cagttgaacg tttaggaaac
    18301 gcgatgaata tcccgacccc aatacgtatc gagtcttttg ataactctaa tatcatgggg
    18361 acgaatcctg tggctgcgat ggtcgtattc attgatggaa aaccggcaag aaaagaatat
    18421 cggaaatata aaataaaaac agtcaacgga ccggatgatt atgcatcaat gagagaagtc
    18481 atctatcgca gatattcgcg agtgattcgc gaagggttgc ctttacctga tttgatcttg
    18541 atcgatggag gaaaaggaca agtagatgtg gcaaaagatg tcttagaaaa tcaattagga
    18601 atcgatattc ctattgctgg catggtaaaa aacgataaac ataaaacaaa tgagttgcta
    18661 ttcggtacag atttggctgt agtaccgctt gagcgaaatt caccagagtt tttcttactc
    18721 cagcgaatcc aagatgaagt ccatcgattc gcaatcactt tccatcggca gctacgcagt
    18781 aaaaacagtt ttgcttcccg attagatgaa attgaagggt taggaccaaa acgaaaaaaa
    18841 atgctgctta aagagttcaa atcgttgaaa aatatcacag cagccacgac agaagaatta
    18901 caagaaatcg gactgccgaa aaatgtcgca caaaacgtat ttgataaatt acatcaggaa
    18961 tgaggaaagg gcactttaca aagaaagtaa agtgtcctat cattttatgt acaacaaaaa
    19021 gagcgtttac gttttgttgg taaataataa atcgcttacg tctggtgtct aaggatatat
    19081 gtgatttctt gaagaaggag tgaagaaatg ttagaagtca aggatcttgt taaaactttt
    19141 gggaactaca cagctgtcga tcatgtctct ttccaaatcc ccgatggaaa aatcatgggg
    19201 ctaattggcc aaaacggggc tgggaagaca acgacttttc gtttgatctt ggattttttg
    19261 acacaagacc aaggagaagt gttatggaat ggccatcatc ttggggagaa agattatgat
    19321 gtcatcggtt atcttccgga ggaacgggga ctttatccga aagtaacgat tcaagaccaa
    19381 ctgctttatt ttgcagaact tagagggaaa tcaagaaaag agattgaacc aaaaatcgac
    19441 ttttggatgg aaaaatttca ggtaaaaggg aaaaagacgg ataaagtaaa atctttgtct
    19501 aaaggaaatc agcaaaaagt ccagctgatc gccacgttaa tccatgaacc taaattgatc
    19561 attttggatg agccattcag cgggttagat ccggtaaatg ctgaattgct aaaagatgga
    19621 atattggaat tgaaaaaaca tggttcatgt gtcatttttt ctagccacaa tatggataat
    19681 gtagaaaaaa tctgcgatca tttgatcatg ttgcgaaacg gaaaaatggt tttgaatgga
    19741 aaagtacatg agattcgtga atcatttggc cgtacgaagt tattcttgga atctggttta
    19801 agccaacagg aagtagaaga aatcgaaggc gtgaaaaaag tgatccttca tgaagacaat
    19861 acgttagaga ttaccttaga agatcctgaa gctgggaaag aaatctttac tcgtgcgact
    19921 cagttcggct acattcctat gtttaaccag caaccgccaa ctttggaaga gattttcaaa
    19981 ttgaaagcag gtgaaatcga tgcgtaagtt ttggattatt accaaagatg tctatttaaa
    20041 aaatgttaag tcgatttctt tcttgatcat gatattagtc ccttttatca tgctgggtat
    20101 catttatgta gcaggaaact ttgctcaaca aaatagtgat gtcaataaaa tcggcgtatt
    20161 ggctgataat gagcaaatca gtcagcaaat gagccaagtc aaaacagatg attttgagtt
    20221 caaagcagtt gattctgaag aaaaagcaca aaaagagctt tcggaagaaa aaatcgatgc
    20281 gtatttagta gtctcaactg agaatgataa gataaatggg atgctttata gcgaaaattc
    20341 attaggacaa agtacacaac tactgataca acaacaactg actggcttgc aatcgatgct
    20401 tcgagcgagt caattaggca tttcaccaga agaagtagct agcttgagcc aacaagcaag
    20461 tttgactcga caaaaagtca attttgattc aaaaggaaaa atgacagtcg gagaagataa
    20521 ctcgacagtc caatacgtcg taagttatgt agcaacgatt gtcttgttca ttattatttt
    20581 gacgtatgcg cagatcattg cacaagagat cgcttctgaa aaaggcacgc ggatcatgga
    20641 agtgatcctg tccagtacga ctgcacaaaa gcatttttac ggaaaattaa caggtgtttt
    20701 acttgttgca ttaacgcaaa tggcattata cggtattatt tttggtatag gatacagtca
    20761 attcaaagat atggaaatgg tcaaaaatat attagacggt atctcattgg ataacttgat
    20821 tggaccattc ttgtggtatt ctcttctatt catgatcttt ggcattttga ttttttctgt
    20881 tttagcagct ttatgtggct ctttggtcaa caaagcagaa gatacagcaa aagcaatttt
    20941 gccagtgact tatctatctc taggcggata catgcttgga ttgatactag gagcttcaga
    21001 tccaaacaat atcgttattc ggatcacttc gtatattcca ttcttatctt catatattat
    21061 gccgattcgc ttagcaaatg agacagtcgg tatgggtgga gcattgattt ccttgttggt
    21121 acttgtagcc gtaacattct tattgatgat cttgtctgca aacatgtaca aatcgaatgt
    21181 actcgtttat agcgaaggcg gattatggag ttcattgaaa caatcgattt caattatgag
    21241 gaatgaacga aaaaaagcat aaaacaatcc taaaatacag aaggaagctg gacatctatt
    21301 gttcccagct tctttttgta tagacctatt ttatttattt tactatattg gatatatatt
    21361 cattgaaaat agtgtataac aactttataa tattgagtat accaattttt ggaggcgttt
    21421 taatggaccg ttatttagat ttactttcag aaaaattccc ctcaacggag gatgtcatca
    21481 cggaaatcat caatctcgaa gcaattttgc agcttccaaa aggaacagag ttatttttga
    21541 gtgatataca tggcgaattt cctgcttttg atcatattct gcgtatcggt tcaggaaatc
    21601 ttaaagaaaa gatcaaagac cttttttctg aacaaatgac ggaaacggat atcagccaat
    21661 tcactatttt tacggcttat ccagaatatg cattaacgac tgattggtac aaagaacaag
    21721 ataaatcgca gctgatccat catttgatcg atcttttacg gtttacgaca gtcaaatata
    21781 cccggtcaaa agttcgaaaa ggattgccga aagaatatag ctatatcatt gaggaactct
    21841 tgtatatcga tgaccgtatc aatggaaaaa aagactatgt aaaaaaaatc attgaacaac
    21901 ttgttttgat gaaggaagaa gaaaaatttt taacgaaact tgcacagacg atccaaaaat
    21961 ctgtcatcga tcatttgcat atcgttggag atatttttga ccgaggaact caagcagcca
    22021 aagtgatgga tcgaatcatg gatttttctt ctgtagatat ccaatgggga aatcatgata
    22081 ttttgtggat tggtgcgtat tgcggctctg aggcatgttt actgacactt ttacggattg
    22141 cagcacgcta taattattta tttgaattag agaaagaata tgggttgaat ttacgtccat
    22201 tgttttcttt tgctcatcaa acttatcaga agaatccagt atttgcgcct aaaaacagta
    22261 aaaatctatc cgaaagagag caagaagaat tagaaaaaat ccatcaggca ctttctatcc
    22321 ttcaattcaa atcagaacat cagttattgg atcgtcgacc tgaatttaaa atggacgatc
    22381 gtaaattgct ggaaaaaatc gactatgaag catcaacgat tgatttagaa ggtcagctct
    22441 atgagttgaa aggaacttgt tttcaaacga tccagccaga tgatccaaaa aaattcttgg
    22501 aagaggaaca aaaagtgatt gatagtttga tgtattcatt ccagcactct ttgagaatgc
    22561 aaaaacatat gacgtttttg atcgaaaaag ggggaatgta tctcacgaac aatcagcatg
    22621 tgctgtttca tggctgtata cctgtagacg agaagggaca gctcttagca tttgaattag
    22681 accaatctta tcgcggaaaa gaactgctgg gcttttttga aaaacagata actgaaagcg
    22741 caaaagatct gactgcaaaa gaagatttag caaccgattt gatttggtat gcgtggagtg
    22801 ggccattttc gcctttattc ggcaagagta aaatggcgac ttttgaacgt tattttctca
    22861 cagagcctca tacgcacgtc gagaaaagca atccttatta tcacttgcga gatgaagagt
    22921 ggttcagtga aaaaatttta gcagaattcg acacaaaaga agaagggtca gcaatcatta
    22981 atgggcacac gccagtaaaa gtaaaaaaag gggagtcacc gatcaaagca aatggaaaag
    23041 tatttgtcat tgacggaggt ttatccaaag cttatcaaaa aacgacagga attgccggtt
    23101 attcactatt atgcaattct tatggtttcc aaatcgtgac acattatccg tttttgccta
    23161 ttgaacaaca atttgcaaaa aaatccgacc aaacaaatgt gaaaaaagta atcgaacagc
    23221 aactaccacg caaactcaat cgtgatacaa caaaaggtat ggaactgcag cagcagatta
    23281 cccagctcaa aaggctgttg gactatcgaa aggacgattg agaagaattc cagtgaacat
    23341 tgttccatag actggagact gagacaaaag tcgtgtcgca gtctcttttt ccggataaac
    23401 ggtgttcttc tgccaactcc tcggtcacaa gtcacactat tcaacaaaca tgaaaagctg
    23461 ttttttgaaa ttgctccttg tgactcagag ttgaacggca ggctcacgac ctcttggtat
    23521 aaacggtgtt caaaagccag tttctcggta ttaagccaaa tttatgcaaa aatgtgaaaa
    23581 gccattttca caaaattttt cttaatactc gaaactaaac agctttttca caaccccctc
    23641 agaaagtcta ttaaaattca ttcacaaaga aatcgaataa gcgctgttca gatggatgta
    23701 cacggtgggt aagttccgga tgccattgaa caccgaggat tttatgcata tcatctttgc
    23761 tttgaaccgc ttcaacgagt ccatcgttag aaaaagcgat ggcttttaat aaaggtgaaa
    23821 gatctttgac tgcctgatga tggtaagaat tcacaaaata tggatcgggg agaaggttgc
    23881 ttaacaaact gtctgaaaca atcttgactt cgtgagtcgt aaattgcggg gcagtgggct
    23941 gttgttcatg ttttactgac cactttggat ataatgaaag atcttgataa agtgtacctt
    24001 ccaaaacgac attcaatagt tggagtcccc ggcagactgc aaaaattggt tttctttgtt
    24061 tcaatgcttc caagatcagt gcctgttcaa attggtctcg ttgaatattt gtttccgtta
    24121 gctttggatg aggatcttcc atgtaaagtt gtggactgac atcttgacca cctgcaagaa
    24181 taagtttgtc gatttgtcca atcagttgag gagctgaatc tggagacatg actggcagaa
    24241 taagaggcaa tccttgagca tctaaaactg catccacgaa tccttgcggt gtatagctca
    24301 cttgatttcc ttggaaagta tctgttgctc gaatcagttg atttccggca atgccgatga
    24361 ttggtttcat ttgtattccc cctaaagtag acttattgta acaaggattt aaaaaaaatt
    24421 aaactatgta agacttgccc ctatacttat ctgttcttgc tgtgttacta tctgtaaaga
    24481 gaaatttcgt gtgaaattca tgacgatagt tgaaaatatt gcttaatttg ttataataaa
    24541 aagtaagcag aaataaaaag gttgggcgta agcttcaagg atctagttct tcaaggggtg
    24601 agaaggacat tttattgaag gggcctgcct atactaagtt atctttagag gtacatactt
    24661 tcaatgaaaa aagaaagcat gtacctcttt tttaaatcga aaggaagtga agcaagtgtt
    24721 agaagctttg gaacaaatcc gaaaagcaga agaagaaaat gaaaagcgtg ctcaaacact
    24781 gcggcagcag ctgaagatct atgcaacaga aaaagagcag gaattggaaa cgatccaaac
    24841 aaactatcga caacaactaa aacagttggt ggaagaaaaa gaacaatttg aaatacaaga
    24901 attagaaaaa gaaaaaatga aattgaaagc agaagcagac aaggtgcaag caactttgaa
    24961 agatcaatat caaaagcatc agagtcaagc gatcggtgcg atcattgaaa gggtgaaaga
    25021 aacttatggc cgtcactaag atggaaaaag tgaccctcat ttcagataaa agaaatcagg
    25081 agaggatttt acaagcagta caaggaatcc aaaacgttga aatccgggat ctgtatcaaa
    25141 atagaacaaa caaccagtgg gtggagaagt attttccaga taccacgatt attgataaag
    25201 aagcaaaatt aagcgcttta ggtaatcggt tgaaagacat ccaagaagct atccaattca
    25261 tacaacacca cggcgatagc cgtcaaaaga aactccattt gaaacgtcaa gaactttcct
    25321 tacaagattt ggaagccaat tattccgaag aaaatttcat aaataaattg aaagaagtcc
    25381 ttgatttaaa agaaaagtgg gaacaactaa ctgaagaacg tgatcgttta gaagaagagg
    25441 aagaatggct tcttcattgg caacagctgg atattgctcc aagtatgtat gcaagtaaga
    25501 cgaccatttt tgagatgggg actgtgagtg cagcaaattt tgaaagtttc aaggaagaac
    25561 tgagtaagct tgatgacgtt tatatggaag aaatagatta tacgccaaaa caagtttatc
    25621 tttcgtatgt tgctttaaaa aaatcagttg ctgttataga agaaattgct gggaaatacg
    25681 ggttcaataa agaaacgtat ccttatgaac aaaatcctaa agaaaaactg caagagacca
    25741 agcagcaatt atccattgtt cacgagcagc aaaagaagat aacgacagcg ttaggtcagt
    25801 gcagtggcta tatccaagaa tttgaatggg tagaagaagt cacacttgcc gttgcagaac
    25861 gagaaaaaat aaaagaacgg tttatccatg cgacttatct gatcgttttg caaggttggg
    25921 tagatgcaga agaaaaacag aatttgctgc atgtgttaaa cgaaaccgta tcagaagaag
    25981 atatatacgt cagtttcgaa acaccaaact cagaagagat tcagcaggaa gtccctacta
    26041 aattgaaaaa ccatccgctt gtcgaacctt ttgaattgct gactgaaatg tacagtctgc
    26101 caaaatatga agaagtagat ccaacacctt ggatgacacc attttatctt gtcttcttcg
    26161 ggatgatggt cgcagatgtt ggttatggtt tgctgatgct cattgggaca atactagctc
    26221 aaaaactact tgttctacca cgcgggatga aacgtttcgt aaaattcttt gagattctct
    26281 cgataccatc gatcatctgg ggattgatct acagttcatt tttcggacag tcattaccaa
    26341 aagaactgtt tggcatccgc ttgcctttcc caatcctatc tacgacggat gacgtcaata
    26401 cgattttgat tttatccgtg attttcggtc tgatacagat tttggttgga ttatttgtgg
    26461 cagcaaagga aaatatcagg agaaaagatt acttgagtgc tgtcagtgat ggatttgcct
    26521 ggcaagggat cctaattggg atcgttattg cgttggttgg tgccatgttg ataaaaaata
    26581 cagcatttgt ttaccttggc gctgggctat cgatcattgc tgccttatgt atcttgatcg
    26641 ttccgattat ccaaacgccg tctaaagtca aaggtgctgc caaaggtgcg tataatcttt
    26701 acggactgac cggctatatc ggtgatttag tcagttatac acgtttgatg gcattgggta
    26761 tttcaggcgg aagtattgga gcggcattca atatgctagt tgctttcatg ccgccagctg
    26821 ctcgattcag tgtagggatc cttttgatca tcgctttaca cgcactgaat atgtttttga
    26881 cactgctaag tgcctatgtc catggcgcac gattgcagta tgtcgaattt tttgggaagt
    26941 tttatacagg aggcggacgt gcctttgatc cgctgaaaac ggcggaaaaa tacgtcaaca
    27001 tcaatcacaa gaaaaaaaac aataaataat tggaggattt ttatataatg atggattact
    27061 taattagtca aaatggtgga atggtatttg cggtattagc gatggcaaca gcgacgattt
    27121 tttcagggat cggttcagct aaaggtgttg gaatgacagg tgaggcagca gcagcattga
    27181 caacaagtca acccgaaaaa ttcgggcagg ctctgattct gcaattgctt ccaggcacgc
    27241 aaggtttgta tggattcgtt attgcctttt tgatttttat caacttagga aatgacatgt
    27301 cagttgtaca aggactgaac tttctaggtg cttctctgcc gattgctttt acaggcttgt
    27361 tttcaggtat tgcccaagga aaagtcgcag cggcaggtat tcaaatttta gcgaaaaaac
    27421 ctgaacatgc tacaaaagga atcatttttg ctgcgatggt tgaaacatac gcgattttag
    27481 gtttcgttat ttccttccta ttagtgttga atgcctaagc ataaaggagg aaaagcagat
    27541 gaacgccatt gaaaaaatca tctctcagat gaacgaagca gcagagcagg aaagagcggc
    27601 gttagaacaa gaagagcgaa tgaagatcga tcaaaacttt gaacaaaaac gtacgcaagt
    27661 tgaaacagaa catcaaaaac aaaaagaaaa acaaatcgaa ttactcgaaa aaaaataccg
    27721 tcagctaagg aatcgccagc aagtagaagt acgacaagaa aatttgaatg cgaagcagga
    27781 atttttaaga cgattgtttg cagatgcggt aactgaaatg gaaaattggg atgaatctga
    27841 acaaatccag tttatcaaaa atgcattgta ttcattacct ttgacaggaa aagtagcatt
    27901 cattgctgga gaaaaatcag cagcctatct ttctcagacg ttgttggatg aatggaacaa
    27961 tgaacttccc tttatgatgg ttttaagtga tgaaactgtt gctgatcaag cgggttttct
    28021 gataaacgat caaggagttc agtataattt tcttttcagc agccttgtac aagatatcca
    28081 aggaacgatg agttttgaga ttgccaatca attattcgaa taaggaggga tcagtgtatg
    28141 gaataccacg aactaaatcc cttgattcgc ggaagagagc ttgaactttt gtccaatgag
    28201 acatttgaac ggatgattca aacaaattct gtcgaagcac ttggtgaaat attgaaatcc
    28261 acgatttaca gtccatatat ttatgacgga tatgaatatg acttcgaaca gaatctttta
    28321 aaggagcaga gccgtctatt tcgctggtta aaggaatcag caccggagcc tgaaatcgtt
    28381 tggatctata cgatgcgtta tactttccat aatctgaagg ttttgacaaa agctgagatg
    28441 acaaatcaaa atctggacca tttgtatatc gacgatggat tttattcatt agaaaccttg
    28501 aaggatgcca ttcatacgca agtttcaaat gaacttcctg aaagcattat ggacagtata
    28561 cgagaagtac gtgattattt tgaagagtct accattcttc agggaattga cgtgatttat
    28621 gatcgacagt ttttgacaga gcagcgacgg cttgccgata agctaggata tcaagaattg
    28681 ctgacagaag tcattgcttt tattgacttg accaatatca caacgatggc tcgaggaata
    28741 ttgcagaaaa gaacatatgg gttcatgacg acggtacttt ccagctcggg aagtatcgaa
    28801 aaagaagtat ttttgaactt tgtagataaa gatatggaag attacacgaa ttttctatta
    28861 gagacagatt atgctgattt tctgcgccct gtcatccgtg agggaaatat tgatctcgta
    28921 aagcttgaac agttgaaaga tgattatcta agttcattgt atcaagaagc acaaacccag
    28981 gctttcggac cactgccact actagccttt ttgaacgcaa aagaggtaga aagcaaaaac
    29041 cttcgtttac tggttatcgg caaaagaagc catttcgaat cagaaacaat cagaaagcgg
    29101 gtgagacaag tctatgacgc ataagatcgg tgtagttggc gacaaagatt ctgttttgcc
    29161 attcaaattg tttggctttg atgttcgtta tggtacaacc aagcaaaccg ttcgtcaggc
    29221 aattgaagaa atggcaaaga atgagtatgg cgtgatttat gtcacagagc aatgtgcagc
    29281 attgagccca gaaacgatcg aacgttacaa ggaacaaatg atacctgcta tcgtattgat
    29341 tcccaatcat caaggatcat tagggatagg gctggaagaa attcaaaaaa acgtagaaaa
    29401 agcagtaggt caaaatatct tatagtatga aaaggagata gaaattttgc aaattggaaa
    29461 aatcataaaa gtctccggac ctcttgtaat ggctgagaac atgtctgatg ccagtatcca
    29521 agacatgtgt ctagtgggag atctaggagt tatcggagaa attatcgaga tgcgtggcga
    29581 tgtggcgtct attcaagtat atgaagagac ttctggaatt ggtccaggcg aaccggttcg
    29641 ctctactggt gaagctttat ctgttgaatt aggaccaggg atcatttcac aaatgtttga
    29701 cggaatccaa cggccattag atacctttat ggaaatcaca caaagtaatt tcttagggcg
    29761 cggagttcaa ttgcccgcat tggaccatga gaaaaaatgg tggttcgaac caacagtaga
    29821 agccggagaa acggtgagtg ctggagatgt tatcgggatc gtagaagaaa caaaagttat
    29881 aaaacataaa atcatggtgc caaatgggat aaaaggaacg atcaaaacaa tcgaaaaagg
    29941 ttcattcact attgatgaaa cagtatgtgt cattgaaacc gaaaatggtg acaaaaattt
    30001 gacgatgctt caaaaatggc cagtaagacg tagtcggcca atcaaagaaa aattaaaccc
    30061 ggatgcaccc atgatcactg gacagcgagt gattgataca ttcttcccag tgacaaaagg
    30121 aggagcagca gctgtacctg gtccttttgg tgcagggaaa acagtcgttc agcatcaaat
    30181 tgcaaaatgg gccgatgtgg acatggttgt ctatgtggga tgtggcgagc gtggaaatga
    30241 gatgacggat gttttaaatg aattccctga attgattgac cctaatacag gagaatcgtt
    30301 gatggaacgt acggtattga ttgctaatac atcaaacatg ccagtagcag ctcgagaagc
    30361 ttctatctat acgggaatca caattgcgga atatttccgt gacatgggtt atgacgttgc
    30421 tatcatggca gactccactt cacgatgggc agaagcactt cgtgagatga gcggacgttt
    30481 ggaagaaatg cctggtgatg aaggttatcc agcttatctt ggttcacggc ttgcggaata
    30541 ctatgaacga tctggaagag tcattgcatt aggatcagaa ggtcgcgaag gaagtataac
    30601 cgcaatcagt gctgtatccc cgtctggagg ggatatttcg gaaccagtca cacaaaatac
    30661 attgcgggtc gtaaaagtat tctggggtct tgattccagt ttagcccaaa aaagacattt
    30721 tccttcgatc gattggatac aaagctattc tctttattca acagaagtag ggaagtatat
    30781 ggatcatatc cttcaagctg actgggcatc catggtcacc gagggcatgc gtattttgca
    30841 ggaagaacag cagctgaatg aaatcgtgcg tttggtaggg attgattccc tttcagataa
    30901 tgaccgtctg acattagaag tggcaaaatc cattcgtgaa gattatttgc agcaaaatgc
    30961 ttttgatgat gtcgatacat tcacttcaag agagaaacaa ttcaaaatgt tgaagatgat
    31021 cttgaccttt ggtgaagaag gacgccgtgc gttgtcgtta ggtgcttatt taagtgagat
    31081 catggacggt accgtatcga ttcgtgaaag gatcggacga agcaaatata tcccggaaac
    31141 agaattagaa aaactagatc aaattattgg tgaaatcaag caaacgatgc aaacgatcgt
    31201 ttcagaagga ggcatgagca atgattaaag aatatcgtac gatcggtgaa gtcgtcggtc
    31261 cattgatggc ggttgaaaaa gttgctggtg tcaaatatga agaattaatc gaagtccgta
    31321 tgcaaaatgg cgagattcgt cgtggacaag tattggaagt ccaagaagat aaagcaatgg
    31381 tccaaatatt cgaaggaaca agtggtatca atttacgtga ctcttctgta cgatttttag
    31441 gccatccttt agaactaggt gtatcggaag acatgatcgg tcgagtattc gatggtttgg
    31501 gacgaccgaa agataacgga ccagagatcc tgcctgaaaa gcatttggat attaacggag
    31561 aagtaatcaa tccagtagct cgggattatc cagatgagtt tatccaaaca ggtatctctg
    31621 caatcgatca tttgaataca ttagttcgtg ggcaaaaatt accagtattt tctggctctg
    31681 gtcttcctca taaagaatta gctgctcaga tcgcgcgaca agcaacggta ctcgattcat
    31741 cagatgattt cgccgtagtc tttgctgcta ttggaatcac ttttgaagaa gctgaatttt
    31801 tcatggaaga ttttcgtcaa acgggcgcaa tcgaccgttc tgtcatgttt atgaatttag
    31861 caaacgaccc tgcaatcgaa cgtatcgcta ctcctcgtat ggctttgact gctgcagaat
    31921 atcttgctta tgaaaaggga atgcatgttt tggttatcat gacagacatg accaactatg
    31981 cagaggcatt gcgagagatt tctgcagcac ggcgagaagt tccaggaaga agaggatatc
    32041 ctgggtatct ttacacgaac ttagctactt tattcgaacg agcaggaaga atccgcggac
    32101 taaaaggatc ggttactcaa attccgattc taacaatgcc agaagacgat aaaactcatc
    32161 caatccctga cttgactggt tatattacag aaggacagat catcttgact agagagcttt
    32221 acaagagtgg gattcagcca ccaattgatg tactcccgtc tctttcgcgg ttaaaagata
    32281 agggaactgg acccggaaag acaagagaag accatgcagc tacgatgaat cagctttttg
    32341 cagcctatgc acaaggaaaa caggcaaaag aattagccgt agttttaggt gaatctgctt
    32401 tatctgatgt agacaagatc tatgcgaagt ttgctgagcg gtttgaaaaa gaatatgtca
    32461 atcaaggatt ctacacgaac cgttcgatta ctgaaacact tgatctaggg tgggaactgc
    32521 tatcgatgtt gccaagaacc gaattaaaac gaatcaaaga cgacatgctg gacaaatatc
    32581 tgccagaagg gaagtgagtt ctgatggcac gattgaatgt gaatccgacc cgcatggaat
    32641 tgactcgatt gaaaaaacag ttgacaactg ccacaagagg gcataaatta ttaaaagata
    32701 agcaagatga actgatgcgt caattcatcc tgttgattcg aaagaacaac cagctgcgtc
    32761 aagaaatgga agagtctatg acagaagcaa tgtctgattt tgtattggca aatgcttcga
    32821 tcacggaacc tttcatcgaa gaattatttg ttttgccggc ggaaaacgtt gatttgtcta
    32881 tcgtagacaa aaatatcatg agtgtaaaag tacctgtgat gaattttgaa tatgatgaac
    32941 agctgacaga cacacctttg gaatacgggt atttgaattc cagtgccgaa ctggatcggt
    33001 caatcgatcg gtttactacc ctgcttccaa aacttttgga actgacagaa atcgaaaaaa
    33061 cttgtcaact gatggcgagt gaaatcgaaa agacacgtcg gcgagtcaat gcattagaat
    33121 atatgacgat ccctcaatta gaagaaacga tctattatat tcgtatgaag ctggaagaaa
    33181 acgagcgcgc agaagtaaca aggttgatca aagtaaaaaa tatgggtacc actgactaag
    33241 tggtatcttt ttttaaaaga ggaaaacgat gattaaacaa agcaaacaaa taaagaaacg
    33301 tttgccggct gttcagcttc tcgcattagg attttttctc ctgattttag ttggcggcgg
    33361 cctacttacc ctaccatttt ttagtaatag tgggcatggg acaaatttta tcgatgcgct
    33421 tttcactgca acttctgctg tctgtgtaac gggattgaca acattgaata cagcagagca
    33481 ttggaacagt gctggtcaat ttttgatcat gcttttgatt gagatcggcg gtcttggttt
    33541 tatgatggtt ccgattctat gctttgcatt gataaagaag aaagtcagtc tcagcacacg
    33601 tattatatta aaagaagcgc tgaatctaga agaagtatca ggtgtgatga agctcatgct
    33661 ttacatccta aaattggcct ttgtgatcca attgattggt acggttgctt tatcttttgt
    33721 atttgtgcct gaatttggct ggttgaaagg tatctggtac agtttgttcc atgcaatctc
    33781 tagtttttgt aatgcagggt ttgatttgtt aggagacagt ttagccaatc accagcaaaa
    33841 tgtttatttg atcatggtgg tatctgcttt gatcatttct ggtgggttag gcttcatcgt
    33901 atggcgtgat ttactggaat atcatcagaa aaagaagatg acgattcatt caaaaatcgc
    33961 attaaccgtt acaggtaccc ttttattagg cggattcgtt gtgttctttt tcacagagcg
    34021 caatggggca acgctagtgg aagggaatat gtttcaacga ttggcaaaca ccttttttat
    34081 gagtgtgaca cctagaacgg ctggatatta ttcagttgat tacctccata tgagccatgc
    34141 aggtctgatt ttaacgatgt ttttgatgta catcggcgga acatctgggt cgacggccgg
    34201 cggattgaaa acaactacgt taggtgtgtt gctgatccag atgcgttcga tgttgaaagg
    34261 acgtacgaga gcggaagcat ttggccgtac catccgacaa ggcgcagtgt tgcgggcact
    34321 tactttattt tttgttacgt tgacgttgtg tgtagtcgcc atcatgattt tatcggtgac
    34381 tgaaacgata cctaaaactt ctggcatcga atatattgct tttgaggtct tctctgcgtt
    34441 tggtacagta ggtcttacga tgggattgac gccagacttg actgcaatag ggaaattgat
    34501 cattatagcg cttatgtaca ttggacgagt tggtgtattg actgtggtct tctctctgat
    34561 gacaaaagca caaagaacac aatcaaaata caaatatcca gaagaaagtg tcatgattgg
    34621 ataatttcat cttctgacag aaacctagtg atttagtaaa ataagaaaat aaaataattt
    34681 ttcttgcttc ttggatactt taagtgcaaa atgttcgttt attttttgag gcactacccg
    34741 ttgttgaacc tcgggtagtg cctctttttt attgaaagga atcaataaga tttgattcct
    34801 ttatttatca ataggttata attcatattt ttttattttt tcttttacga tacttagtcc
    34861 agaagctaaa atttcaaggt tattagagga tagggaaatt cgaacgaatg catctggttg
    34921 cgcacgtgta gtaaagcgtc tggaatgata aagtcggata ccgttagcaa gaaagtcatt
    34981 ctcaattatc ttagggtcaa tctcaggatc aactggaaga attcgatagt aagggacacg
    35041 attcgttggt ttttctactt tgaaaagaga atcaaaaagc tgattggatc tttttgtaag
    35101 acttaattta gtttctaaga tcctatttgc ttcatttgaa tggaggattt gagtgatgac
    35161 ttccacatct aatccagaag ttttcacatt tatattaaaa attgcttgtt ctatcttgct
    35221 ttggacatgt actggaacta caagataagc aactcgcaat ccagaacaaa taaattttgt
    35281 cattccagct aagtagattg tttggttagg taataaattt tggaaaggag gaatgatcgt
    35341 atgttgatag taagttgtta aaaatgaatg gatatcatct tcaataaccc atagaccttc
    35401 tttttcaatc acttcggcta attgctctcg tcttttgtga gagatttgaa accctatagg
    35461 attattacaa gaaggcataa gaaagacacc atgaatcttc ttttttctac attcctgaat
    35521 caatatatca ggtcgcattc cttgctcgtc aaaatcaatt ggaacgatct ctaaatgata
    35581 taactgagcc aattcaatga aattagcgta tgtatatcgg tctgttgcta ttctatcacc
    35641 tggagagaaa agtgcagaaa gtgtgatagc caatccattt tgaacaccgg aaacaataga
    35701 tagagattct aaggacggaa tgactccttg agtttttaac caatttgctg cagtagtcaa
    35761 ttgaaaattt gtgcctttgg gatcttggta attaagcaaa ccaacaagat ttgggttatt
    35821 actagttgat tgaataaacg ggacaacgaa tttattacat tcttcaaaag aactaaccag
    35881 accaaaatca atgatatgtt cggttacttc attggtagag atggtgatgg actcaaagac
    35941 attgggagaa ataaatgtgc cacttccaat attcgtatac agcaaacctt tctcaattcc
    36001 atatttataa gccttgccaa cggtagtaaa atttaaatct aaataatcag ctagctctct
    36061 ttgggagggt aactgagtgt ttttctgtaa tttattcttt aaaatatctt gttctaattg
    36121 ttgaatcaac gatcgataat aaggacgctt caatttttgt ttgtctggtc gccaagttaa
    36181 cgggtaattg tcataagaat taataggcat tttctccact ccttaaaatt gtataccata
    36241 caattttacc attgattgta ttgattttag aagttaatct tttgtgagga gggaattgtc
    36301 tatgaaaatt attaattttg aggaagtagt agcttctttt agttttgaag aagcgattca
    36361 agtaatgaaa aaatgttttt ttgattatga aagtggtaaa atcagccaaa atcctcgatt
    36421 ggtggaaatt ttacctgacg gaaaaggcaa aaatatgttt gcgatgatgc ctgcttattt
    36481 gggggagaac agatattttg gcacaaaagt cattaccgct tttccggata atcatcagta
    36541 tcatttacct tctcatttag gagagatttt cttgtttgat gctcaaaacg gactacccaa
    36601 agcattgata aacgcaaatg cagtcacctg gattcgaaca gctgctgtat ctgcgctggc
    36661 aacagattac ttagcaaatc cgcatgcaga aacacttgct ttaattggag ctggtcaaca
    36721 agcattgtcg catttgactg cgatttctac cattcgtccc atcactacag tgtttgttta
    36781 cgatatttta gaagaaagac gaaatacttt tatcgctaaa gctaaaaaac agtatcctaa
    36841 aataacgttt ataaatgcaa tatcagtaga agatgcagta cgtccagcgg aaatcatttg
    36901 tacgttgacc tctagtaaag aggcatttct tcaaaaagag tgggtaaggg aagacgcaca
    36961 tatcaatgct gtgggcacat ttactcctga tacaagagag ataagtacat cgttgatgga
    37021 aacttctcaa atctatgtag atgattactc agctgcctta acagaaagcg gggacctgct
    37081 tatagccatg gcagaaggag atttagagga atcttgtatt gttggtacat taggcgaatt
    37141 agtttctgag aaaaaaacat tttataaagg agaatcgagg ccgacgattt tcgacgcagt
    37201 tggtctagct attgaagatc tttgttgtgc agaatatatt tataacaaat tagagaaagg
    37261 ggctttaaaa tgaacttaga ttattacgta gttgatgcat ttgcagatga ggtgttcaaa
    37321 ggaaatccag cagcggttta tgtgctagaa gaatggctgc cagaaggtac gatgcaaaaa
    37381 attgccattg aaaacaattt atctgaaaca gcatttacag tgaagaaaaa ccaagaattt
    37441 gagcttagat ggtttacgcc agatcgagaa atcgatcttt gtggtcacgc gacattagca
    37501 acagcatttg ttttatttaa ctattacaaa atacctgatg aaacgattaa attttctact
    37561 caaagcggta atctatttgt gacaagaatt aaagattatt attacatgga ttttcctagt
    37621 attatgccga aaaaagtgcc gatattgtct gaatatgaag aagcgattgg ggctaagatt
    37681 aaagaagcct acttagcacg tgatttattt ttcgtattag aagatgaaaa aacggtggct
    37741 aaattgcagc cagattttac agctatcaaa aattttgaat taggaatagg agtcatcgtg
    37801 acagcagaaa gtgtgcaaaa agactttgtt tctagaacct tctttccaaa attaacaatc
    37861 aatgaagatc cagtatgtgg atcagcacat tctaatctta ttccttattg ggcagaaaga
    37921 atgaataaaa caaaattgtc agcttatcaa atgtcatcaa ggggcgggga tctagaatgt
    37981 gaattaaaag aagatcgtgt gataatagga ggaaaagcag ttttgttttc taaaggtacc
    38041 tcgtttattt gatttctttc aaatgaatta aaaataaatt ggtggagagt ggcaacctta
    38101 tagctacaat ggttgttaaa aaaagacgca aactttttca atttaatgaa aagtttgcgt
    38161 ctcattttaa ttattggtgt ttgtttttta ttcaatcaag agtaaaatgc cccatactaa
    38221 ctatcctcgg atgacttcga ttttataacc atcagggtca atgacaaagt agtaagaagg
    38281 cgcagtgcca ggtaatcctt ttaagtcagt aatctcaaga ccagcagctt tatgttgctc
    38341 gtgtaatttt tctagatcat cagctgctat agcgatatga ccatagccgt ctcctagatc
    38401 atacgcacca tgatcatagt tgtaagtcaa ctctaattca tagtcatccc caggaagtgt
    38461 caaataaaca agtgtaaatt tgttttctgg aaaatcacgg cggcggcttt cttcaaaacc
    38521 aaatgccttt tgataaaatt cgattgatgc ttcaagatct ttcactcgaa cacatgtgtg
    38581 agccattttc atagattcat ctctcctttt ccttttactc aatcataaca aaaagaagta
    38641 aggaaatagt tgatttgtgc ttgttcatga gaaatttctc atgtaaaatg gatagaaaat
    38701 aaaactgtta aggtggaaac tattcatgga taaaccaatt tttggtgaaa aaaagcaatt
    38761 tgctgattat caaacccgtt atgcagcata tattgtcata gaaagagatc agcagattgc
    38821 agtaatcgag gctccaaatg gtgctttttt tcttcctggc ggggaaattg aaggagaaga
    38881 gacgaaagaa gaagcgattg tccgtgagct gatagaagaa atgggaattg tggcagaaat
    38941 ttcttcttat ctgggagaag ctgatgagta tttttactcg aattatcgtg caacttatta
    39001 ttataatcca ggatatttct ttgttgctga ttcttggaag cgtataggag aaccaactga
    39061 aaagaccaat aaaatttggt gggtgagccc tcaagaagcg cttgtgaaat tgaaacgtgg
    39121 tagccaccga tgggcagtcg agagatggat cgaaaaaaat aaaaactgaa agaaagcgag
    39181 tagatcagct ttctctcagt ttttttattt attacagtac atattttttg attgcttcag
    39241 ctacaccgtg ctcgtcgttt gatgaagtga tgacatttgc tgcttctttg acaagagaaa
    39301 cggcatttcc cattgctact cctagaccag catattcaat catggataag tcgttctcgt
    39361 tatctccgat agccatgatt tcatcttgat caataccaag gtgctgtgcg agattcgcaa
    39421 ctgccgcacc tttacttgct tctttattta gtacttcgaa gtaatagggc gcacttttga
    39481 ccgttgtgta tttatcacgg aaagtttgcg gtaaacgcgc aattgctgca tctaaaattt
    39541 ctggttcgtc gatcatcatc attttgacga tgttcatgtc tggtgtcatt tcttcaggtg
    39601 ttcgatattt tagcggcatc ttgactaaac ttgcttcgtg gatcgtataa gcgctgatat
    39661 ctcgattagg cgtgtagatc gctttgtcat cgatcgaatg gagatgactg ccgacacggc
    39721 gtgccattac ttcgatctct aggaaatctt catgtgtcag tccgtgacgg gcgatgattt
    39781 tacctgattt cgtttgttgt acgagtgccc cgttataagt aataacgtaa tcattatctt
    39841 gaaaaaggtt caactcgttt aactgttcct ggacacctgg taagggacgg ccagtgcata
    39901 aaacgatcga aacacctttt gcaacagcag cggcaatcgc ttcttttact tcttttgtta
    39961 cttctctttt ctcatttaat aaagtaccat cgatgtcgat agcgactagt ttgatagaca
    40021 taatttcctc caatatattt taatttattt caatcaatgc gccattacgg atatggctgg
    40081 caaattcttg ataagtttcg tcaaataaat cataatgatc acgcaaggac ggatcaacca
    40141 tttcttttgg aaagaaaaat cgctcatctc cttgttcttg tcccgctaat gcagcaacaa
    40201 gctgactaac ctgtgataat tctatcaaag ttccgtcatt tcgcaaaatc tcgatttgtg
    40261 tgcgatgtct gccttgttct gggcgataaa aatcataagg tagatcgtag ctagaattca
    40321 cagcagtgta ataaacagga ttatatccga ccttttctac taacaaagtg agttcttgga
    40381 ctaattttga atcccggttg ccagaaaaag tagcagattt cagtggttta cgattcaaaa
    40441 aacgtttagc taaatcattt aaaataggat cagcagattc agaccattga gtaaagtaag
    40501 tgcccaagac cccatcatca agtttgaggt attcttctaa agtaaagctt ccgtcaagaa
    40561 atggcagaag taattgagaa tggagttcaa aactgctggc atcttcttga tacaactcgt
    40621 gtgcgcgatg aagcagatgc tccaaaatta cttccatacc acgggaaaca ggatggaagt
    40681 acacttgcac atacatttga tatcggctaa cgatataatc ttccactgca tgcatgccac
    40741 tcatggaaaa agcgatcccg ccttcataag gacgaatcac acgtaatatt cgggtaagat
    40801 caaatgttcc atattctgtt ccagtgaaat aagcatcccg aagcaaatag tccattcgat
    40861 ctgcatcgat ctgactagaa atcatttgga caacttgtgg gttggggtaa gttttttgga
    40921 tcacactagc taccttttca ggaaaacctt cttctactcg gttaagaatc tgatacactt
    40981 ctgtttctgg agaagtgatg atctctacag tgatggcttc atgattcgta tggaagatat
    41041 gttcaaatgt atgagagtaa ggaccatgac cgacatcatg caacagtgcc gcacataaag
    41101 tcaccagccg ttcctgatca tcccagcctc cgttgcctat tttttctttt gaaaaatttc
    41161 gagaaaagat atcgcagatt cgtcttgaaa tctcataaac gcctaatgaa tgagtaaaac
    41221 ggctatgttc tgctccgtga aaagtgaaag aagaagtgcc taattgttta atgcgtcgta
    41281 gacgttggac ttctttcgaa ttgatcaaat ctaagattac ttgatgctgg acatggatat
    41341 agttatggac tggatcacga aaaacttttt ccataggcag catttgatat ttataagggg
    41401 ttgtcataat gtagctcctt tatgttatta cgttgttcca ttctctggat ttggttgttc
    41461 cattgctcca gctggatttc tttttttagc cagtcggtag aatcctgtaa atcacttgca
    41521 gaatacagct tttcaaaagc ttttgcaaat gcttctttga cttcttttac aaaaaggggt
    41581 tggtcaagca gttcttcaag tgttgccatt gtagcaggat ccactgcagg atagccattt
    41641 gtaccaaagt cttcttttaa agcagattga taaaatcgct tcatgacttg cccacgtttt
    41701 tcctgattac cgttgacact taagtacatc ataacagaaa tcccattttt gatccgacgc
    41761 tgagcgatac cggcaaattt tttgccatgg atgcttaaat cataagttcc aggacaataa
    41821 gaatgggtga tttcgaatgc ttgtatcggt gcctttggaa aagcgtattt agtcaatagg
    41881 agcatttgct cgtaagcgtc atctatggtt attttttttg tgtcactttg ggggaaaatc
    41941 aaagaaatat tcaaaattcc ttcatctgat acgatcccta atccacctga atttctgatc
    42001 actggagcgt aacctgcatc ttccagtgtt tggatcccgt cacttagaaa aggagttcga
    42061 acatccttca tacctaagat gactgtgcag tcatattgcc agaaatgtaa aaagatttgc
    42121 tggtgttctt tagaatattc cgttaatata tctgttaatg caaaagggga aaagtcgttt
    42181 ttttgataaa tgtgctgatc tagtcgaatc gcagggttca ttcaaccggc tcctttgtat
    42241 aaattcatat tttctacaat attatactct aaaatcaaag agttgttttg gaaaaaaggt
    42301 tttctgtaaa aatggacaag ttggttgaca aaacaaaaga aactaggaac aatgtagcta
    42361 tgagaagtaa aggagaatga ataatggaat tatcacaagg aacaccgatt tcaatcaaat
    42421 tgcgtacaaa agtaaaacag caaggggaag tgcaagattt ttattttgat ttgaaagggc
    42481 aaatggtgac aattggcgat acgctgtaca tacgttataa agagattcag gaggagacgg
    42541 gcgatgagat tcctgtaacg atcaaattag ttccagatgg tcatgtacag ctgatccgtg
    42601 caggagaaat gcggatgcgt ttgaaatttg gctataaaga acggttagaa acatcctatc
    42661 gaacaccata cggtatgatc cagattgcta cttttacgaa agaactgcat gttagtttga
    42721 aagaccgtcc cacagcaggg aaggttcgaa tcgattatga tctgttcatg ggaccggaaa
    42781 aaatcggcga atattatttg accttggatt ttacagcgta aatataaaag aaaagtttga
    42841 ttttttgtgt ctgattttgt atgataagga gtagactgtg aaaggacgtg tcctgattgg
    42901 aaattagtgt atttgaaggt gcaaacaaaa gcgaattatc aatgatcgaa gtagcacacg
    42961 cgatcttaga acaacgtggc gatgtcatgg atttttctga tttagctaac cagatccaaa
    43021 actatcttgg gaaatcagac agtgaaattc gtgattcact cgcacaattt tatactgact
    43081 tgaatattga cggcagcttt atctcattag gggataatcg ttggggcttg cgttcatggt
    43141 atgctatcga ttcgatcgat gaagaagtaa accatggaat ggacgaagag gatgaagata
    43201 ctccacgtcg ccgtaaacgc aagaaagtca acgcctttat caatgatgat gaagacgcga
    43261 tcgattacaa tgacgatgac ccagaagata cagatctgac agaagaagat gacgatgatc
    43321 tgtttgatga cgatgatgat gaagatgaag aaatcgctgc ttataattct gacttgcaag
    43381 aaatcggagc agatgacccg acagatgatg aagaagactt gccaggaaat atcgaagaag
    43441 atttaagtat catagacgaa gacgatgatg atgaagatta tgaagaggaa gaattttctg
    43501 actctgaaaa agaataaaca ttattttctt acgagaggtt gtgacagaga cgatcagcct
    43561 atcagaataa ggaggtgtga cacgattttt
//
LOCUS       DACDPS010000012        43363 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.012, whole genome shotgun sequence.
ACCESSION   DACDPS010000012 DACDPS010000000
VERSION     DACDPS010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.012"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            591..1280
                     /locus_tag="IWJ07_000767"
     CDS             591..1280
                     /locus_tag="IWJ07_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9332366.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="IWJ07_000768"
     CDS             1267..2475
                     /locus_tag="IWJ07_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9332367.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="IWJ07_000769"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="IWJ07_000769"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAP9332368.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="IWJ07_000770"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="IWJ07_000770"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAP9332369.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="IWJ07_000771"
     CDS             complement(6578..7273)
                     /locus_tag="IWJ07_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAP9332370.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="IWJ07_000772"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="IWJ07_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAP9332371.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="IWJ07_000773"
     CDS             complement(8472..8663)
                     /locus_tag="IWJ07_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAP9332372.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="IWJ07_000774"
     CDS             complement(8684..9574)
                     /locus_tag="IWJ07_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAP9332373.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="IWJ07_000775"
     CDS             complement(9561..10328)
                     /locus_tag="IWJ07_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP9332374.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="IWJ07_000776"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="IWJ07_000776"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAP9332375.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="IWJ07_000777"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="IWJ07_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAP9332376.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="IWJ07_000778"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="IWJ07_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAP9332377.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14944..15282
                     /locus_tag="IWJ07_000779"
     CDS             14944..15282
                     /locus_tag="IWJ07_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332378.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(15450..16208)
                     /locus_tag="IWJ07_000780"
     CDS             complement(15450..16208)
                     /locus_tag="IWJ07_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP9332379.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="IWJ07_000781"
     CDS             complement(16226..17044)
                     /locus_tag="IWJ07_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP9332380.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="IWJ07_000782"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="IWJ07_000782"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAP9332381.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="IWJ07_000783"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="IWJ07_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAP9332382.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="IWJ07_000784"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="IWJ07_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAP9332383.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="IWJ07_000785"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="IWJ07_000785"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAP9332384.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21063..21305
                     /gene="yaaA"
                     /locus_tag="IWJ07_000786"
     CDS             21063..21305
                     /gene="yaaA"
                     /locus_tag="IWJ07_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAP9332385.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21292..22416
                     /gene="recF"
                     /locus_tag="IWJ07_000787"
     CDS             21292..22416
                     /gene="recF"
                     /locus_tag="IWJ07_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAP9332386.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22413..24359
                     /gene="gyrB"
                     /locus_tag="IWJ07_000788"
     CDS             22413..24359
                     /gene="gyrB"
                     /locus_tag="IWJ07_000788"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAP9332387.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24379..26850
                     /gene="gyrA"
                     /locus_tag="IWJ07_000789"
     CDS             24379..26850
                     /gene="gyrA"
                     /locus_tag="IWJ07_000789"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAP9332388.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27086..27385
                     /locus_tag="IWJ07_000790"
     CDS             27086..27385
                     /locus_tag="IWJ07_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAP9332389.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27435..27947
                     /gene="ssb"
                     /locus_tag="IWJ07_000791"
     CDS             27435..27947
                     /gene="ssb"
                     /locus_tag="IWJ07_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP9332390.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27973..28209
                     /gene="rpsR"
                     /locus_tag="IWJ07_000792"
     CDS             27973..28209
                     /gene="rpsR"
                     /locus_tag="IWJ07_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAP9332391.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28352..30325
                     /locus_tag="IWJ07_000793"
     CDS             28352..30325
                     /locus_tag="IWJ07_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAP9332392.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30331..30783
                     /gene="rplI"
                     /locus_tag="IWJ07_000794"
     CDS             30331..30783
                     /gene="rplI"
                     /locus_tag="IWJ07_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAP9332393.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31057..32424
                     /gene="dnaB"
                     /locus_tag="IWJ07_000795"
     CDS             31057..32424
                     /gene="dnaB"
                     /locus_tag="IWJ07_000795"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAP9332394.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33204..34496
                     /locus_tag="IWJ07_000796"
     CDS             33204..34496
                     /locus_tag="IWJ07_000796"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAP9332395.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            35283..36146
                     /locus_tag="IWJ07_000797"
     CDS             35283..36146
                     /locus_tag="IWJ07_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9332396.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(36275..37897)
                     /locus_tag="IWJ07_000798"
     CDS             complement(36275..37897)
                     /locus_tag="IWJ07_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9332397.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            38146..38667
                     /locus_tag="IWJ07_000799"
     CDS             38146..38667
                     /locus_tag="IWJ07_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332398.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(38853..40583)
                     /gene="spxB"
                     /locus_tag="IWJ07_000800"
     CDS             complement(38853..40583)
                     /gene="spxB"
                     /locus_tag="IWJ07_000800"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAP9332399.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            40748..41566
                     /locus_tag="IWJ07_000801"
     CDS             40748..41566
                     /locus_tag="IWJ07_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAP9332400.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(41669..41866)
                     /locus_tag="IWJ07_000802"
     CDS             complement(41669..41866)
                     /locus_tag="IWJ07_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332401.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            42035..42613
                     /locus_tag="IWJ07_000803"
     CDS             42035..42613
                     /locus_tag="IWJ07_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP9332402.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
BASE COUNT        13207 a         8295 c         8553 g        13308 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tgtattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    20941 tttaaatcac cttttttaga ttaaatcgtc ttatatggaa aaatatatgg ataaatttgt
    21001 tttattgcga aaaaaaaggt ataatagagt atacttttaa taatgaaaat gagggatggg
    21061 cattgaaaca acaagtcatt ctaaatacgg aattcatgac gttgggccaa atgttgaaag
    21121 aagtcacagt tatcggtagt ggcggacaag caaaatggta tttggcagaa aacactgtgt
    21181 tagtcgatgg cgaaccagaa aatcgtcgtg gacgcaaact ttatcccggc atgatggttg
    21241 aagttccaga agtaggaact ttttttatgg taaaaaaagg aaacgacgat aatgaggctg
    21301 aataagctgt atttaaaaaa ttatcgaaat tacgaagagc tgaacatcga attttccaag
    21361 aacctcgtca tctttttagg agaaaatgcg caaggaaaaa cgaatctttt ggaaagtatc
    21421 tacgtattag ctatgacgcg aagtcatcgg acaagtagtg agcaggaatt gattggctgg
    21481 actgacgatc aggcgatgat tcaaggagag atcacaaaag gttcatccac tcttccatta
    21541 gaaatcttat tatctaaaaa aggccgaaaa accaagatca accatatcga acagaaaaag
    21601 ttaagtactt acgtgggaca aatgaatgtc attttatttg caccagaaga tctttcttta
    21661 gtaaaaggca gcccgcagat caggcggaag tttttagata tggaattagg ccagatcaat
    21721 ccaatctatc tttatgatct tgtccaatac caagcaattt tgaaacaaag aaaccagtac
    21781 ttgaagcaat tgaatgaaaa aaaacaaact gatttgcttt atttggatat tttgtccgaa
    21841 caactagcag cgtttggcag caaagtattg aaagcaagag cacagttcgt caagcgattg
    21901 gagttttggg ctaattcttt gcatcagcaa atcacccatc aaaaagagca gctggaaatc
    21961 gagtatctca ccgcagtcga ttctttagaa acacatacgc aagagcaaat ccaagaacaa
    22021 tttctcgcac ttttgaacca aaataaaaag aaagaccttt ttcggggaac aacgacagtt
    22081 ggtcctcatc gtgacgatct ttcctttttt attaatcaga aaaatgtgca gacttatgga
    22141 tcgcaaggac agcaaagaac aactgcacta agtgtcaaat tagcagaaat cgatttgatt
    22201 aaagaagaga caggggaata tccaatcctt ttgttagatg atgtgatgag cgagctggat
    22261 gataatcgtc aactccatct gctagaaaca atcgaaggaa aagttcaaac gttcttaaca
    22321 acaacgacac ttgaccatgt aaaaaataaa atgactgttg aacctgagat tttttatgtt
    22381 caacaaggga aactaaaagg agaagaaact gaatgacaga agaaagaagt ttagtagaac
    22441 gcgctaaaga gtatgatgcc agtcagattc aagtacttga aggacttgaa gccgttcgta
    22501 aacgacctgg catgtatatc ggttcgacta gttctgaggg tctgcatcac cttgtttggg
    22561 aaattgtcga taattcgatc gatgaagtat tagcaggttt tgcaacgaaa atccatgtga
    22621 ttattgaaaa agacaacagt atcaccgtta ttgacgacgg tcgggggata cctgtagata
    22681 tccaggcaaa aactggacgt cctgcagttg aaacggtctt tactgtttta catgctggag
    22741 gaaaattcgg cggtggtggg tacaaagttt ctggtggact tcacggagta ggatcttctg
    22801 ttgtaaacgc gctttctaca cagcttgatg tcaaagtata caaagaagga aatgtgtact
    22861 atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa gattatcgaa caaacggatc
    22921 gccatggaac aacggttcac tttacgccag accctgaaat ttttacagag acaactgagt
    22981 ttgatttttc aaaattggct acccggatcc gtgaacttgc tttcttgaac cgcgggatgc
    23041 gtatatcgat tgaagacaaa cgagaagaag aaccagtcat caatgaatac cattatgacg
    23101 gcgggatcaa aagttatgtc gaatacttga atgcaaacaa aaccgtgatt tttccagaac
    23161 ctgtatattt ggaaggtgaa cagcaagata tcgcagttga agtctctatg caatatacag
    23221 acggctacca ttccaatatc atgagttttg ccaacaatat ccatacgtat gaaggtggaa
    23281 cacatgaatc aggattcaag actgctttaa ctcgtgtgat aaatgattat gcccgaaaac
    23341 aaaagctgat gaaagaaaat gacgacaact tgaccggtga agacgtgcga gaaggattga
    23401 cagctgttat ttctatcaag catcctgacc ctcaattcga aggacaaaca aaaacaaaat
    23461 tgggcaattc cgaagtacgg acagtcactg atcggttatt tagcgaacat ttcatgaaat
    23521 tcttgctgga aaacccaagt gtgggccggc aaattgttga aaaaggatta ctagcttcca
    23581 gagctcgtct agcagcgaaa cgtgcgcgtg aagtaacgag acgtaaaggt gctttagaga
    23641 ttagtaatct gccaggaaaa ttagcggact gctctagcaa cgatcctgaa aaatgcgaat
    23701 tgttcatcgt cgaaggagat tcggccggcg gatcagctaa gcaaggtcgt aatcgagagt
    23761 ttcaagccat cttgcctatt cgtgggaaaa tcttgaatgt tgaaaaagca agcatggaca
    23821 agatcttggc aaatgaagag atccggtctt tatttactgc tatgggtaca gggtttggag
    23881 aagattttga tgtatcgaaa gctcgctacc acaaattagt tatcatgact gatgccgatg
    23941 tcgacggcgc gcatatccgt acattgctgc tgactctttt ttatcgttac atgcgtccta
    24001 ttgttgaagc tggatacgtc tatattgctc agccaccttt atacggggta aaacaaggga
    24061 agaatattac gtatgtacaa ccaggaaaaa atgcagaaga agagttgaaa caagtcgttg
    24121 cttcacttcc tgcctcacca aaaccaagcg ttcagcgtta taaaggactt ggagaaatgg
    24181 atgatcatca actatgggaa acaactatgg acccaagcaa tcggatgatg gcccgcgtca
    24241 gtgtggacga tgctattgca gcagatcaga tatttgaaat gttgatgggg gatcgagtag
    24301 aaccccgtcg tgcattcatc gaggaaaatg cacactatgt gaagaactta gatatttaat
    24361 catgaaggag gaaaaagaat gagtgaagaa atcagagaaa acatccatga tgtcaatcta
    24421 accagcgaaa tgaaagactc cttcatcgat tatgcgatga gtgttattgt cgcacgtgca
    24481 cttcctgatg tcagagatgg tctgaaaccc gttcatcgcc gtattttata cggcatgaac
    24541 gaattaggtg taacaccgga taaagcccat aaaaaatctg ccagaatcgt cggagatgtc
    24601 atgggtaaat accatcctca tggagacatt gcgatctatg agtcaatggt acggatggca
    24661 cagccattca gctaccgtta tatgctagtg gatggtcatg gaaactttgg ttctgttgac
    24721 ggtgacggtg ccgccgctat gcgttatacc gaagcacgga tgagcaaaat cgctacagaa
    24781 atgctgcgtg atatcaataa gaacactgtt gatttccaaa gcaactatga tgatacagaa
    24841 aaagaaccag ttgtactacc tgctcgcttc ccgaatcttt tagtgaacgg aacaacgggg
    24901 atcgccgtag gtatggccac gaatatccca cctcataact tgtctgaggt agtagcggct
    24961 atcgatttgc tgatggataa tcctgatgtg acgaccaatg aattgatgga agtattacct
    25021 ggaccagatt ttccaacagg tggtttggtt atggggaaat caggaatccg aagagcgtat
    25081 gaaacaggta agggttctat taccgtacgt gcaaaagttg agattactga gatgccaaat
    25141 ggtaaagaac ggatcatcgt gacagaattg ccttatatgg tcaataaagc aaaattgatc
    25201 gaacggattt ctgaactgca cagagacaag cgtatcgaag ggatcacaga tttgcgggac
    25261 gaatcttccc gtgaaggtat gcggatcgtc attgacatcc gtcgtgacgc cagcgcatcc
    25321 gttatcttga ataacttata taaattgaca tccttgcaaa attcatttgg attcaatatg
    25381 ttggcgatcg aaaaaggtgt gccgaaagtt ttgagcttga aacaaatcct tgaaaactat
    25441 atcgaacatc aacgcgaagt catcacacga cggacggtct ttgaaaaaga aaaagcagaa
    25501 gcaagagcac acatcttaga aggtttgcgt attgctttgg atcatatcga tgaaatcatt
    25561 gcgattatcc gcgggtctaa atcagatgac gaggcaaaag caacaatgat cgaacggttc
    25621 gatttatccg atcgccagtc tcaagctatt ttagatatgc gtttacgccg attgaccggt
    25681 ttggaacggg aaaaaatcga aaatgaatac caagaactct taaaactgat tgaagattta
    25741 actgacattc tggcaagacc agaacgtgtt acagaaatca tcaagaatga attagcagac
    25801 ttgaatcaac gatttggtga taaacgacgc acagaattac tagtcggtga ggtcttaagt
    25861 ctggaagatg aagatttaat cgaagaagaa gaaatcgtta ttacattgac aaacaatggt
    25921 tacatcaaac gtttggcaaa taatgaattc agagctcaac gaaggggtgg acgtggtgtc
    25981 caaggaatgg gtatccacaa tgacgacttc gtcaaaaatc tggtttcttg ttctacccat
    26041 gatacattgt tattctttac gaataacgga aaagtgtatc gggcaaaagg atacgaaatt
    26101 ccagaatacg ggcgtacagc caaaggaatc ccaatcatca atttactagg tatcgattcg
    26161 agtgaaagca tccaggcaat cattgcggtg acgggagaag cagaagaagg ccactatctc
    26221 ttctttacga cgcgtcaagg tacagtaaaa cgtacttctg tcactgcttt tgctaacatc
    26281 cgaagtaatg gtctgatcgc aattggtttg aaagaagacg atgagctcgt caatgtgcta
    26341 ctgacagatg gtcaatctaa tatcattatt ggaacacata acggctactc tgtcactttc
    26401 aaagaagaag cagtccgcga tatgggacgt acagcttccg gggtcagagg gatacgcctg
    26461 cgtgaggaag attatgtcgt tggggcagcc ttaatgaagg aaaatcaaga agtattagtg
    26521 atcacagaaa aaggttacgg aaaacggaca aaagtatcgg aatatccagt gaaaggccgc
    26581 ggaggtaaag ggatcaaaac ggcgaacatc actgaaaaaa atggtccact agctggattg
    26641 accactgttt ctggagatga agacatcatg ctgattacag ataaaggtgt cattatccgt
    26701 ttcaacgtat cgactgtttc tcaaactgga cgttcaacat taggcgtacg tttgatgaag
    26761 atggaagctg atacgaaagt tgtcacgatg gcagcagtcg aaccagaagc tgcagaacaa
    26821 acagttgaag aacaaggatc agaagaataa ccatcaagac taagtgtgta agctagcaag
    26881 aataaccgat taaaaaatct ggttattcct gctggctttt ttgattagtt gaatttctct
    26941 taaaatattc gaaactgttc attctgtatt gcttttgacc aaaatagagt gtataattag
    27001 agcttgtgag tagtctaatt aattagataa ctctccttgc tcttaggaac ctaagagccg
    27061 aaagtccaca gggaggtgaa tatcaatgga aaacacgaaa tatgaaatca tgtacattat
    27121 tcgtccaaac attgatgaag aagcaaaaac tgctttaatc gaacgtttcg atgcaatctt
    27181 gaaagataac ggagcagaag ttatcgaatc aaaagattgg gaaaaacgcc gtttcgctta
    27241 tgaaatgaac ggttaccgtg aaggtatcta tcacatcgtt aaagtatctt ctccatcatc
    27301 agcaaatgct gtcaatgaat ttgaccgtct tgctaaaatc aacgacgata tcattcgtca
    27361 catgattgtt aaagaagaag cttaattgtt gtttcatgtg aaacaataga aaacgatgag
    27421 aggagatgac tattttgatt aataatgttg tattagttgg acgtctgacg aaagatccag
    27481 atttgcgtta cacagcaagt ggtacggcag tcgcaacatt tactttggct gtgaatcgta
    27541 attttacaaa ccaaagcggc aatcgcgagg ctgattttat caactgtgtg atttggcgca
    27601 aatcagcaga aactttagca aactatgctc gtaaaggaac acttttaggt gttactggac
    27661 gtatccaaac tcgttcttat gataatcagc aaggacaacg tgtatacgtg acagaagtag
    27721 tagctgacaa cttccaattg ctggaaagtc gttctgcatc tgaaaatcgc caacaaagcg
    27781 gcggattcca aagttctggt caatcagctg gcggatttgg cggaaacaat aactctaatc
    27841 aaacatctca atcatccaac ggtatgcctg atttcgaccg tgatacttct gacccattcg
    27901 gctcaagttc aacgatcgac atttcagacg atgatttacc attctaaacg aatttgatag
    27961 gagggaaata gaatggcaca acaaagaaga ggcggacgta aacgtcgtaa agtagactac
    28021 attgctgcta accatattga atatatcgat tataaagata tcgaattact aaaaagattt
    28081 atttcagaac gcggtaaaat cttgccacgt cgtgtaactg gtacaggcgc taaaaaccaa
    28141 cgtaaaatta ctgttgcaat caaacgcgct cgtatcatgg gcttgctacc tttcgttagc
    28201 gaagactaat aaatcctagg aattgaatga ctgatccttt tgatcagcta ttcaattcct
    28261 ttttttctgt aaaaataacc tctattcgtt tagtcgctcg aatgacgaaa gttatggtaa
    28321 aatcaaatta gtcatatatg ggaggaaaat tatggaaaat aaaaacaatc atttatcccc
    28381 gaaacctctc ttccttatcc tgtttatatt aatcgaagta ttggtagtct taattttacc
    28441 atttcgtttt gttagcgctt cggtcattct attgatgaat ttatggggtg ttcgctattg
    28501 gttgaaaatg aaacgttggc ttattttaaa taaacgtgaa aaaatccagc aggcttcgac
    28561 tgtagctgaa accaatctag cttatgtctc agaagtcatg cctgtaggag tgattgctta
    28621 tcagccatca agccaaaaaa tcgaatggct caatccatta gcattgaaaa tcaaagatca
    28681 aagtggatta acagaggaag aattgctaga tacttttttt actgcaaaag aaaaaaagaa
    28741 aaaccaagtc tatctagcag ataaaacatt tcagtttgat atggatacag taaagggtgt
    28801 tgtttatttt aatgatatta cccaaaacca gttgttaaaa ctccatcaga cgaacagtca
    28861 gccggtgatt ggtattttat ctattgataa ctatgcagat gccattgata aattagatga
    28921 caaagagatt tcttatttga actctttcgt cactacacta gtttctgact ggatgaatga
    28981 ataccatgtc tttttcaaaa gaatcaacgc agaacggttt ttcttcgtag cacagatgat
    29041 cgatctgaaa aagatgatgg ataaaaaatt tgatttgctg gatcgctttc gagcagaagc
    29101 tgaaaaacag agctcgccta tcacccttag tatggggatt gcttatggct caagaaccct
    29161 cgatgaaatc ggtggagaag ctcagaataa tttagatgtt gctttagtcc gtggaggcga
    29221 ccaagttgtc gtgaaagagg taaaagaagg agcaaaacct gtttattatg gggggaaatc
    29281 tgctaagaat gtcaaacgca cgcgtgttcg ttctcgagcg atgagtaccg ctttgcgtcg
    29341 gatttttgat gaaacaggcg atgtgtttat catggggcac aagtttccgg atatggatgc
    29401 tttaggctct gcttttggtg tcagttgtct ggcacgcttc aaccagaaaa aagcttacat
    29461 cgttatcaat gagaacgaga tcattccaga tgtagaacgg tgtcttgaag aaatccataa
    29521 gcatcctgaa ttagaaatgc aactaatctc tccagaacgt gccatagagc tgaaaaaaga
    29581 agaggacctc ctcgtgatgg tggattacca taaaccttct ctatcgatct ctcagggcct
    29641 atatgagcaa tttgataaga ttgtgatcat tgatcaccat cgtcgtgggg aagagtttcc
    29701 aagtaaacct ctgctgactt atattgaatc ttcagcttct tcagcttcag agctagtttc
    29761 tgagctgatc caataccaaa gcagcaaaac aaatcgctta gatcgttttg aggccacatt
    29821 attgcttgca gggatggttg tagatacaaa tagtttcaat gtccgtacaa ctgcacgaac
    29881 ttttgacgtt gccagctatt taaggtcttg tggagcagac gcatccttag tacaatattt
    29941 attgagctcg gatttagcga catacttgga gatcagcaat ttgattgctg gaagcgacta
    30001 tgtgacttct gatattgttg tggcggcagg gacagacgaa aaagagtata atagtgttat
    30061 agcagcaaaa acagcagata cattgctttc gatgatcaat atcaatgcag ctttcgtcat
    30121 tacaaagcgg acagacggct tgattgggat cagtgccaga agtaccggca cgatcaacgt
    30181 ccaaatcatc atggaatcct taggtggcgg aggacacttc acaaacgcag ctactcaact
    30241 ggaaaatacg acagttgagg aaacaaaaca gaagctattt gctgcaatca aagaaaatat
    30301 gaatcaaata tatgataagg agtaatccaa atgaaagtaa tttttttaca agatgtaaaa
    30361 ggaaaaggta aaaaagggga catcaaagaa gttccagcag gttatgcaca aaattatcta
    30421 atcaaaaata acttggcaaa agaagcaacg aaaagcagta tcagtcagtt agcaggacaa
    30481 aagaaagcac aagaaaaaca cgaagctgaa atcttggaag aagctaaaaa gttaaaagaa
    30541 ttcttggaaa aagaagatac tgtagtcgaa ttgaaggcga aagcagggga agacagccgt
    30601 ttgtttggtt cgatcccttc aaaacaaatt gctgaagggt taaacaaaca atacaaaatc
    30661 aaattagata aacgaaaaat cgaattagaa aatccgatcc gttcactagg ctacacaaac
    30721 gtacctgtta agcttcatca tgaggtgacg gctacgatca aagttcatgt cgtacaagaa
    30781 taacaaatct tgatagcgat gttcagctgg taagtataac cagaaggaaa aaagacaaac
    30841 cgaaatttat ctgagaaaac agctgcagca atgaaagcaa aagctgcaaa tcgatttcag
    30901 attgatttcg gtttcttttt tgggaacagc aaaatagcat aaagaaaacg agtataataa
    30961 aagagaacta tttttttatt acactttcgg tacgaatgat gacttttctc tcattttctt
    31021 gtaaaatgaa gcaattgaag acaaggagcg atacgtatga atgaagtgtg gcaggatcga
    31081 atcccaccgc aaaatattga agctgaacaa gcggttttag gctctgtttt tcttgacgca
    31141 gaaacgatca ttgatgccat ggagtacata gagccaaaag acttttatcg tcgtggccat
    31201 caaattattt ttgagacaat gatcgagtta aatgatcgta atgaagcgat tgacgtggtt
    31261 actgtcaaag accggctgga acaagccaat ttgttagaag atgccggtgg tttaagttat
    31321 ctctcagact tggcattagc agttccgaca gcagcaaata tcgtctacta tgcgaaaatc
    31381 gttgaagaaa aatcattgct gcgtacattg atccagacag caacaggaat cgttacgaaa
    31441 ggattcgagc aaggagaaga agttcaaaca attttagatg atgccgaacg cagtatcttg
    31501 gaggtttcgg aaaaaagaaa tcgaagcggc tttttatcta ttgctgaagt actgaactcc
    31561 acgattgaaa atatcgatcg actagctcaa aacaatgaag aaatcacagg tctaccaact
    31621 ggctatcaag gtctggataa aatgacagca ggtttccagc ccgaagaact aatcattctt
    31681 gctgcacgtc cagcggtagg taaaacagcg tttgcattga atattgctca aaatgtagga
    31741 acaaaaacag atcgttctgt tgcgatattt agtttggaaa tgggggcaga atctcttgta
    31801 aatcggatgc tttgtgcaga aggttcgatt gaggccagcc atttacgtac aggtcagctt
    31861 tcggaagaag aatggtcgaa tctgatcgtt gcgatgggga gtctttctcg agcaaatatt
    31921 tttatcgatg atacaccagg gatcaaaatc tctgaaatcc gtgcgaaatg tcggaaacta
    31981 gcgcaagaaa aaggcaatct aggcttgata ttgatcgatt atttgcagtt gattgaaggt
    32041 aacggtaaag agaaccgtca acaagaagta tcagatatct ctcgccaact gaaaaaactg
    32101 gcgaaagagc tgaaagtacc tgtcattgct ctttcacagt tatctcgtgg agtagaacag
    32161 cgacaagaca aacgaccagt gctaagtgat attcgtgaat ccggatcaat tgaacaagat
    32221 gcggacatcg tagccttctt gtatcgagat gattattatg aaagagaagg cggagatgac
    32281 gatgatggaa acgagccaaa aaatgacaac gtcatcgaag tcatcatcga gaagaaccga
    32341 agcggtgcac gaggaacagt tgaactactg tttatcaaag aatacaataa gttttcgtcg
    32401 ctttcgccaa gagaagaatt ttaaagaaga ggttgtgagc cttgagcaat cccgagagct
    32461 ctcgtaagaa gatgcaagga gtaaccgcag gaacgactag agtacttaat ttaaacagat
    32521 ctccaaaggg attgtgacaa aagtcttgtc acagtccctt tttccgaata aacggtgttc
    32581 aatcagctga cccttggtat tagctcagca aacgtcaaaa tatgaggaac tattttgacg
    32641 tttgtttctc taatactcag gtcaaaacgc tgatcttacg acctcttttc cttaaacggt
    32701 gttctcctgc caactcctcg gtcacaagtc acaatatcat caaacatgag aagcagtttt
    32761 ttgaaattgc tccttgtgac tcagagttaa acggcaggct cacaacctct ttcttaaacg
    32821 gtgttcaaaa gcttgcatct gcggtaatac gcccaatcaa acgaaaaata tgagaagcta
    32881 tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac ctctgattca
    32941 cggtgttcca aaagtagctt cttcgaaaaa taagtcgaaa tttcacaaaa atttgagaag
    33001 caattttcgt acattccttc ttatttcttg aagctgacca cttctgttac aacctctttt
    33061 ttaataacca ttctaaaaag ataaagttcg ttttttaagc gtttttataa aataaaaata
    33121 aaaatcattc gattttattt gaaatatgta tctattattg atacaatggt tcaggattaa
    33181 ataatataga acgaggtgtt cacatgtcat ctgtagtagt agtaggtacg caatggggcg
    33241 atgaagggaa aggaaaaatc acagactttc tcagtgaaaa tgctgaagtg attgcacggt
    33301 accaaggtgg cgacaatgca gggcacacaa ttaagtttaa cggtgtgact tataaactgc
    33361 atttgattcc ttctgggatt ttttataaag acaagatcag tgtgatcgga aacggcgttg
    33421 tagtcaatcc taaatcattg gttaaagaat tagcttattt acatgaaaaa ggtgtagaaa
    33481 caagtaattt gcgtatttct gaccgcgcgc acgtaatctt gccttatcat atcaaattgg
    33541 accaattaca agaagatgct aagggcgaaa ataagattgg tacaacgatt aaaggaatcg
    33601 gacctgccta tatggataaa gcagctcgtg taggtatccg tattgcagac ttattagata
    33661 aagaaatttt tgaagaacgt cttcgtttga atctagatga caaaaaccgt caatttgtaa
    33721 aaatgtttga ttcagaacca cttgattttg acgatatttt tgaagaatat tatgaatacg
    33781 gacaacaaat caaacaatac gtcacagata cttccgttat cttgaatgat gctttggatg
    33841 caggcaaacg cgtgttgttt gaaggcgcac aaggtgtaat gctggatatc gaccagggaa
    33901 cttatccttt tgttacttct tctaacccag tagccggtgg ggtaacaatc ggaagcggtg
    33961 tagggccttc aaaaatcaat aaagttgtcg gcgtctgcaa agcctatacg tcacgtgtcg
    34021 gtgatggacc atttccaact gaattatttg atgaaacagg agaacaaatc cgcgaagtag
    34081 gacgagaata cggtacgaca actggtcgtc cgcgtcgtgt aggctggttt gattctgtag
    34141 ttatgcgcca ttctaaacga gtttcaggta ttaccaacct atctttaaac tcgatcgatg
    34201 tattaagtgg attggaaaca gtaaaaattt gtacggctta tgaattagat ggcgaactaa
    34261 tctaccacta tccagcaagc ttgaaagagt tgaaccgctg caaaccaatc tatgaagaat
    34321 tgccaggctg gtcagaagac attacaggat gcaaaacact tgcagaactg ccagaaaacg
    34381 cacgtaacta tgtacgtcgt atctcagagc ttgttggtgt acgtatctca acattttctg
    34441 tcggacctga tagaacacaa acaaacatct tagaaagcgt ctgggcgcaa atctaaggat
    34501 aaaaacgacg actgaatcag gacgttctta ccagagcgag tcgaagtgtt tatctatacg
    34561 ataataataa cttgaagagg tagtgaaaga gacgtttagc cttattgccg ataggctgtc
    34621 tcttgaacac tgtttattca gaataaggga ctgtgacaaa aaccgtgtca cagtcccttt
    34681 ttccgaacaa acggtgttca aaagcttgca tctacggcac taagcccaat cagatgaaaa
    34741 atatggagtg ctatttttat ctgattgttc ttactgcttg atgcagagca gctttttcac
    34801 aacctctcca ctaaacggtg ttctcctgcc aactcctcgg tcacaagtca caatattcaa
    34861 caaacatgag aagctgtttt ttgaaattgt tccttgtgac tcggagctaa acggcaggct
    34921 cacaacctct tggtaaacgg tgttcaatca gctgaccctc ggtattagct caaaaaacgg
    34981 caaaatatga ggaactattt tttccgtttt tttctctaat actcaggtca aaacgctgat
    35041 ttcacaacct ctgattcacg gtgttccaga agtagcttct acggaaataa gccgaaattt
    35101 cacaaaaatt tgagaagcaa ttttcgtaaa ttccttctta tttcttgaag ctgaccactt
    35161 ctgtcacaac ctcttatttt gattagaact tgcgaaaata aaccggttaa agtgtgtcta
    35221 gcgcaagaga tttgctataa tagacgataa gggtaaaatg taacgagaag gggttgaaaa
    35281 atgtgtatca aattgtaaca gattcttgtt gtgatctgcc ataccatgta ttagcaaaag
    35341 aaaaagttga ttttttatca atgaatctcc aattgaatga tcaagagtta gtagatgacc
    35401 tcggaaagac attcaattat gaccgctaca tgaatcagct gaaaaatggt gcgatgccta
    35461 cgacatcaca aatcaatgtt ggccgatatt tagaattttt ccgttcttat gcggaaaaac
    35521 agattcctgt tttatatgtg gctttttcca gtggattaag cggctcttac agtagtgcgc
    35581 ttcaagcggt tgaattgctg aaggaagaat atgaagaccc tgagatctat gtttttgata
    35641 caaaagctgc aagtattggg gaagggcttt tagtattgga agctgtaaga ttgaaaaaag
    35701 aaggtaaaag catcgaagaa accctagagt ggctggaaag taattatttg aaagtccatt
    35761 catgggtaac agtagacgat ctgaaatatc tagaacgagg cggtaggatt tccaaagcaa
    35821 gtgctactct tggcagtcta ttgagtgtaa agcctattat tacggtggat aaagaaggaa
    35881 ggctgacgaa tgttgacaaa gttcgaggaa gaaacaagtc gatccagaca atcgtcaatg
    35941 agacggtaaa aacaatcgtt gcaccacttg atcaaatcgt ttatgtggct tacgctgggg
    36001 atctagagag cgcggagaag gcaaaacggc ttttggaaga gcagataaag atgaaagatg
    36061 tgaagcttta tcccttagga ccaacgatcg ccagtcacac aggctatggc tgtatcgcga
    36121 ttttttcgat gggaatcagt cgataaaagc agtaactcat tctgtaaaaa agcccagaaa
    36181 ttgattgtaa caaaaacagc gactcttaaa agatagatga gcatcctatc tcttagaggt
    36241 cgctgttttt ttatttttta agctaaatag gccattatgc ccataactcg ttcaattgtt
    36301 tttgaatatc tgcattttcc aaaaattcat catatgtcgt atcagctcga tcgatcacac
    36361 ctttatccga aacggcaatg atgcggtttg ccaatgtttg gataaattga tggtcatgtg
    36421 acgcaaataa gattgagcca gtaaatgcca tcaagccgtc attcaatgct gtgattgatt
    36481 ctaagtctaa atggttcgtt ggatcatcta atacaagtac gttagctttc gaaagcatca
    36541 tttttgaaag catgcaacgg actttctctc caccagataa aacattcact ggtttcaata
    36601 cttcttcacc agaaaacaac attcttccta agaagcttct taagaaggtg ttatcatctt
    36661 cttctttact tgcatattga cgcaaccagt ctaaaatcgt tagatcagta tcgaattctt
    36721 tcgttgtatc ctttggtaaa taagaattac ttgttgtcac accccaacgt actgttccag
    36781 tatctggcgt aatttcaccc atgataactt tgaatagcgt tgttgttgtt atatcatttt
    36841 ctgcgacgaa agcgaccttg tcttctttgc tcaaattaaa agatatatca tctaaaattt
    36901 tctttccatc aatcgttaca cttacatttt ctacctgcaa taagtcgttt ccaatctcac
    36961 gttcaggttt gaatccaacg aatggataac gtctagaaga aggttggata tcgtctaaag
    37021 taattttttc aagcattttt ttacgtgaag ttgcttgttt tgattttgat gcattcgcac
    37081 taaagcgggc aataaattct tggagttctt tgatttgttc ttcttttttg gcatttgcgt
    37141 ttgcttgaag tttagccgct agttgactag attccaacca aaaatcataa tttcctacat
    37201 aaagtttgat tttaccaaaa tctagatccg ccatatgtgt acatactttg ttcaaaaagt
    37261 gacggtcatg ggatacaacg atcacagtat tttcaaaatt gatcaaaaat tcttccagcc
    37321 aattgatcga ttggatatcc agaccgtttg ttggttcgtc tagcaaaaga acatcaggtt
    37381 taccaaatag tgtttgtgct aataacactt ttaccttttg tccaccagtt aattcactca
    37441 tttgctgatg gtgcaattct tcagggatgt tcagaccttg cagtaaaaca gctgcttctg
    37501 gttcagcttc ccatccatta agctcagcaa attctccttc taattcagct gctcgaatac
    37561 catcttcgtc agtgaaatcc tctttcatgt aaatcgcatc tttttctttc agcacttcat
    37621 acagacgctt gtgtcccatg atgactgttt caagtacagt atagtcttca tagtcgtaat
    37681 ggttctgttt caatacggcc atccgttcat ctggtcccat agtaacggaa cctgtggaag
    37741 gctgcagatc ccctgataac actttaagga acgttgattt acctgcacca tttgcaccaa
    37801 tcaagccata gcagtttccg ggggtaaatt taatattcac atcatcaaat agtttgcggt
    37861 ctgaaaagtg caaactcaca tcatttacag taatcaattt ttttcctcac ttttatcatt
    37921 attagttttg acaagtctta tagaattata taggggaaag gtaaaagatt caaggcaatc
    37981 gaacattctt tgtgtttcac gtgaaacaga atgaaaaaaa taaaaataaa agcggagcga
    38041 aaatagctac tataggattt attagtttgc aacagcataa tttttgacgt tatttataag
    38101 agggagtact ataaaactgt aaaagaaatg aaagaaggat tgaaaatgga tgaaaaaaga
    38161 accatcgact ggggctcatt gattttggga attttatttg tacttgtctc attgttatca
    38221 tttcaagacc ctgtcggaaa tttagttgca attgtagtag tctttgcgat ttttgccttt
    38281 attaaaggga tatttgaact gtttgtacgg aacagattaa aagagctaac aggatataaa
    38341 ggaaagatgc ccttaataat cgggatcatc gatattctag ttggcgtctt tttcttattc
    38401 aatattggtg caggagtcgc agcattgcct tttgtttttg cagtatggtt tattgctgat
    38461 tcagttttag cgttatttac tgctgacttg gcaagaggag taagtgaagg atattattgg
    38521 tttacgatca tcgtcaatat tttaggaatt ttattaggaa ttttcttatt gtttaatcca
    38581 atttcttctg cgttgacatt aagcttttta gttggattct attttatgtt atttggtatc
    38641 acacatatcg tctatgcatt tagataagta aaatcaaaca aataaaaaag ccggcttccg
    38701 attacagttt tactgttgta attggaagcc ggctttttta tcatggacaa atatgcacga
    38761 ggtgattttt aggtgtcgag acaaatttat gcaaaaatgt gagaagctat atacacaaat
    38821 tttatcttaa tactcgaagc atagcgcagt tgtcacaacc tcttttcata taattcattc
    38881 aatactggca aatcatggac ttcatatttt tctttgaatg cttggatttc ttcttcagaa
    38941 tattttcttg gatctaaatg cagatcttct actggcaatg ggcgcttgtt attgatcttc
    39001 acatcgatta ctacaggacg atctgatttt gcagctgcat caaaggcaaa tcgaagttgc
    39061 gagtattctg tgactgtaaa gctatttgcc ccgagcgctt ctccaacttt accgaaatct
    39121 gcatcttcta aaaatacacc aaatttcttt tgttccgtat cttcttgttc tgcttcgatg
    39181 aaaccaaaag aatcattcga aaagacaaca ttaataattg gaagctgata tttgacttgt
    39241 gtaatgatat cttgcatgac catagcaaac ccgccatcac cacttaaggt aaatacttgt
    39301 ttttctgggt agcttagttg tgcggcaatt cctcctggca caccattacc catagttgcg
    39361 aaccatccag atgtcgtatg tttttgttta ccgttcatat tcaataaacg aatagaatgg
    39421 atagtcgtgt taccgacatc tgtgacgaaa atcgcatcgt cttcagcaat acgattgatt
    39481 tctttataga cagcctctgg acgaatcggt tcttcctctc gatcttcaaa agatttcaac
    39541 caatttaccc atttttcttt attttcttga tttgctaggt accagctatc agcagggcga
    39601 gcatccccta actcgacaag tctttgcaaa gctgtgtttg catcaccgag gatcgacaaa
    39661 gatgtttcat ggcgtcgtcc aaattttgac gcatcgatat cgatttggat aaattcagca
    39721 gttggattaa aaaatgcacg tccaaatggg aagtcgctac cgacaaataa gatcagatcg
    39781 gcttctgcca atgcttcatt tccgggttta gtagcgacac gtccagcaaa accaagaaag
    39841 ttttcataat catcaggaat aattcctttt gctagaacgg aagcgacaac aggaactgaa
    39901 aaatgttctg agaattcttt gatttgcgga aatccgtttc gcgttccttg accaacataa
    39961 agaactggtt tttttgcttt ttcaagataa ggcaaagcag ctagcaaatc tttttcttct
    40021 ggtaatatca cacttgtttt gtgagtgtga gcggtggaga aaggttgttc gtcaatttct
    40081 tcaaaaccaa gatcaacagg gatagtcacg atagccacgc ctttatgctc ataggcggct
    40141 ttaatggctt catctactac atgcggaaga ctttgtggtg tcatgactgt acgattgtat
    40201 acgctaacat ctgcgaaaat aggattttca tttagttcct ggaaagagtt atagttcatg
    40261 gatgtagaag caacttgtcc taagaacgct actagaggca catggtccat ctgtgcatca
    40321 tacaaaccat tgatcaaatg tgtcgcacca ggtcctgcag agccgaagac agcgccgact
    40381 ttccccgtta attttgcatc tgcagcggct gcgagtgcgc cgacttcttc atgacgaact
    40441 tgaatgtagt tgatttcggt tttttcttga tatagcgcat ccataattga attgaaagat
    40501 cctccgggaa tgccgtaaat atggtcgata ccccagcttt caagaacttt taccattgcg
    40561 attcctgcat tgatttttga catggtgttt cactccttta gcaatgatga tacataaatt
    40621 ttttgtatct agagtataga actttttatc gaaaatccca actaattcgt cttttcacaa
    40681 aaatgtagtc atgtttttgg gaatatgcta gagtagtaca tagatagatt caagagaggg
    40741 gaatggcatg gttgaatggc tattgagact tgaaccttgg cagcaagcac ttgctgggac
    40801 agcttttact tattttatga cagctcttgg tgccgggttg gtttttttct ttaaagaaat
    40861 caaaaaagaa gtgctaaact tgatgttagg atttgcttct ggtgtcatga ttgctgcaag
    40921 tttttggtct ttgttagatc cggctattac aaaagcagaa gaaaatggag atattgcttg
    40981 gctagttgta agtattgggt ttggtttagg cggtgtgttt ttatatatgg cagataaaac
    41041 actgccccat atgcattttg gaccacaaca tgaagcagaa ggattgccta cccatttaaa
    41101 acggacgatc ttgcttgttt tttctattac attgcataac attcctgaag gtttagccgt
    41161 gggggtagca ttcggtgcag cagcaactgc ggataatccg acagccgcta tattagccgc
    41221 tgtttcggtg gcgctaggta ttggtatcca aaacttccca gaaggagcag ctgtatcaat
    41281 cccgttgcga caagaaggtc tgagtcgtaa aaaggccttt gtttacggac aagcttctgg
    41341 aatcgttgaa ccaattgctg gagtgattgg agcgctacta gtaacgaaag tggagctgct
    41401 gcttccttat gcacttgctt ttgccgcagg agcaatgatt tatgtcgtgg tggaagaatt
    41461 gatccctgaa gcacagcaga cattgtccag caaacgtcat tttgcagttt ttggcgtcat
    41521 gagtggattt atcattatga tgattttgga tgtggcatta ggataaataa aaaaaatata
    41581 tatgatatat caaatagggc cgcgaactta tatcgcgacc ctattttttt agtaagttat
    41641 atcataatga agtgaatggt atcatacatt aataatagtt ttttctatgt gtatttcctt
    41701 tgatccagcg aacaagccag atgataagtg cgattgggat tagaactttg accgcaaacc
    41761 ataaaagccc gcttaggaca ctgacaaaaa gactgatgac gaagctagct gcaattagca
    41821 ctaaaagaac catgaagata tttaagaaca tatttttttg acgcatacaa aaccctcctc
    41881 ttgttcattc tttactatag aaaaagtata acagggattg tttaaaaaca cctccaactg
    41941 aagactgatt tttacttcat ctacttaaat gatcaaacaa ttgctttttc tctggtttat
    42001 catctaaaat agagcggaaa aggaggagaa tatcatgcct aaaaatcgtc ttgaagcatt
    42061 tacagatgct gtaatcgcga ttatcatgac tattttagtt ttagaacttc atgaacctaa
    42121 cggagacacg tgggaggcgc tcgctgcatt aggttaccga ttttttatct atattgtcag
    42181 ttttatcatt ttagcgattt attggaacaa tcatcatcat ttatttcaag ttgtacataa
    42241 gataaatgga cgggtcttat gggcgaacag tttctttgtt tttgcgcttt cgctttttcc
    42301 gtttgcaact tcatggatca gtgagtacat caatagcctg gctccagaaa tgacttatgg
    42361 ttttgttacc ttgaccgcta atattgcgta ttatatcttg gcaaaagaac ttgtcaaagc
    42421 aaataaaggc cacgagaaag cattgaaggc gatgttgaat aattacaaga aatcgtatct
    42481 atcgattggt ttgaatgtgt taggaattgt cttagggtat ttagtagcgc cagtagccgt
    42541 gctgatcgca aacatcgtga ttctagctgc ttggatcatc ccaagtcgta ccatcgaatc
    42601 acaatatcgg taaaatgatc cgtgtttaaa atttaagaga aaaatataga aaaaataaat
    42661 aaacgatcaa atatccgagt gatactagta ttcaaagagg gatgcagatg gatcaaaaaa
    42721 gaattcatgt aggactgatt actagttttg tcacgatgct tatttatttg gatcaaggtc
    42781 atttgacttg gacagtagtt ggtgtcgtag gatttttgac attattcaca gcaataaaaa
    42841 aataaacatc tttatgtaat ctgatgttct atctatttaa gaaaagcatg tttacagcag
    42901 tatgattctg ttgtaaacgt gtttttattt gtggaaaatt taatttttag aaaagtgtat
    42961 cgaaaaatag atagttagtt tttccacagt ggataacttc gtaagagata aaaataaaaa
    43021 attagtcaaa gaagggcgta tagatttctt ttgataggag atatacaaca gaaatgaata
    43081 aatattaacg tgttttcaaa cagtataaaa cggtgtggtt tttggaataa aaaaataaca
    43141 tatctcattt tacacttttg ttattttata cttaaaaaat aacgacacag aaaataccgt
    43201 gtctcagatt tctctactac ttctaaaaac tactgttttt ttcctttgaa atgccttcaa
    43261 aaagtaggat caggcagaat atactggcag aatcaaggag aagggagcct aaataaggag
    43321 aaaaaccata gaaaatataa agtaattact tataaaacaa gct
//
LOCUS       DACDPS010000013        42333 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.013, whole genome shotgun sequence.
ACCESSION   DACDPS010000013 DACDPS010000000
VERSION     DACDPS010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.013"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(72..1640)
                     /locus_tag="IWJ07_000804"
     CDS             complement(72..1640)
                     /locus_tag="IWJ07_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAP9332403.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(1644..2054)
                     /locus_tag="IWJ07_000805"
     CDS             complement(1644..2054)
                     /locus_tag="IWJ07_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP9332404.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(2051..2974)
                     /locus_tag="IWJ07_000806"
     CDS             complement(2051..2974)
                     /locus_tag="IWJ07_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAP9332405.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(2971..3849)
                     /locus_tag="IWJ07_000807"
     CDS             complement(2971..3849)
                     /locus_tag="IWJ07_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP9332406.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(3836..5173)
                     /locus_tag="IWJ07_000808"
     CDS             complement(3836..5173)
                     /locus_tag="IWJ07_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP9332407.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(5176..6279)
                     /locus_tag="IWJ07_000809"
     CDS             complement(5176..6279)
                     /locus_tag="IWJ07_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP9332408.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(6353..7861)
                     /locus_tag="IWJ07_000810"
     CDS             complement(6353..7861)
                     /locus_tag="IWJ07_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9332409.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(7827..8519)
                     /locus_tag="IWJ07_000811"
     CDS             complement(7827..8519)
                     /locus_tag="IWJ07_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP9332410.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(8541..9515)
                     /locus_tag="IWJ07_000812"
     CDS             complement(8541..9515)
                     /locus_tag="IWJ07_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9332411.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            9815..10687
                     /locus_tag="IWJ07_000813"
     CDS             9815..10687
                     /locus_tag="IWJ07_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9332412.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(11145..13343)
                     /locus_tag="IWJ07_000814"
     CDS             complement(11145..13343)
                     /locus_tag="IWJ07_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9332413.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            13575..14567
                     /locus_tag="IWJ07_000815"
     CDS             13575..14567
                     /locus_tag="IWJ07_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9332414.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            14627..16048
                     /locus_tag="IWJ07_000816"
     CDS             14627..16048
                     /locus_tag="IWJ07_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAP9332415.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            16041..17540
                     /locus_tag="IWJ07_000817"
     CDS             16041..17540
                     /locus_tag="IWJ07_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAP9332416.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            17579..18973
                     /gene="uxaC"
                     /locus_tag="IWJ07_000818"
     CDS             17579..18973
                     /gene="uxaC"
                     /locus_tag="IWJ07_000818"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP9332417.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            19132..19815
                     /locus_tag="IWJ07_000819"
     CDS             19132..19815
                     /locus_tag="IWJ07_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAP9332418.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            19817..20653
                     /locus_tag="IWJ07_000820"
     CDS             19817..20653
                     /locus_tag="IWJ07_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP9332419.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            20664..22418
                     /locus_tag="IWJ07_000821"
     CDS             20664..22418
                     /locus_tag="IWJ07_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9332420.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(22694..23035)
                     /locus_tag="IWJ07_000822"
     CDS             complement(22694..23035)
                     /locus_tag="IWJ07_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP9332421.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(23103..25274)
                     /locus_tag="IWJ07_000823"
     CDS             complement(23103..25274)
                     /locus_tag="IWJ07_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP9332422.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            25485..26342
                     /locus_tag="IWJ07_000824"
     CDS             25485..26342
                     /locus_tag="IWJ07_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP9332423.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(26413..27270)
                     /locus_tag="IWJ07_000825"
     CDS             complement(26413..27270)
                     /locus_tag="IWJ07_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9332424.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(27255..29957)
                     /locus_tag="IWJ07_000826"
     CDS             complement(27255..29957)
                     /locus_tag="IWJ07_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP9332425.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(29970..31259)
                     /locus_tag="IWJ07_000827"
     CDS             complement(29970..31259)
                     /locus_tag="IWJ07_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAP9332426.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            31409..32455
                     /locus_tag="IWJ07_000828"
     CDS             31409..32455
                     /locus_tag="IWJ07_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9332427.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            32457..34610
                     /locus_tag="IWJ07_000829"
     CDS             32457..34610
                     /locus_tag="IWJ07_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP9332428.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(35083..36534)
                     /locus_tag="IWJ07_000830"
     CDS             complement(35083..36534)
                     /locus_tag="IWJ07_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9332429.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(36531..38255)
                     /locus_tag="IWJ07_000831"
     CDS             complement(36531..38255)
                     /locus_tag="IWJ07_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9332430.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            complement(38248..38862)
                     /locus_tag="IWJ07_000832"
     CDS             complement(38248..38862)
                     /locus_tag="IWJ07_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP9332431.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            complement(39207..40664)
                     /locus_tag="IWJ07_000833"
     CDS             complement(39207..40664)
                     /locus_tag="IWJ07_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9332432.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            complement(40705..41625)
                     /locus_tag="IWJ07_000834"
     CDS             complement(40705..41625)
                     /locus_tag="IWJ07_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9332433.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            complement(41637..>42333)
                     /locus_tag="IWJ07_000835"
     CDS             complement(41637..>42333)
                     /locus_tag="IWJ07_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9332434.1"
                     /translation="KNTILYNVAFILLNLLISVVFAIIMSELRNKRLVKVYQTMSLLP
                     YFLSWVIISYFVYAFLSPDKGIFNQWITGHGGEAVNWYNEPKYWPFILVFIGTWKGIG
                     YNSIIYFASVMGIDPTYYEAAMVDGASKWQQIKHVTIPQLIPLMTILTILAVGNIFRA
                     DFGLFYNIPRNSGALYDVTQVLDTYIYNGLTSTGDFGMTAAAGLYQSVVGFVLLMITN
                     TIARRFDSDSALF"
BASE COUNT        12425 a         8662 c         7525 g        13721 t
ORIGIN      
        1 atatatacaa acaaaaaaat tcactaacta gtgaaaataa gttagtgaac ctcatttatt
       61 aaagaattaa attacagatg atcttttcca tcatacttga aaaactcaaa atcagcataa
      121 cttcctcttt ttttatttag atcatgcact gctatcccga taaaatttcc agtaaaccct
      181 cctgagagta tacacatgtt tttttgcgga cctactttct tccattcttc agttgatacg
      241 tctttcaaat gaaactgtgc tgtctctttc tttacagtga ctttcaaaaa agtcttcttt
      301 tctgtgagtg gaatcgtcca atcttctatt tgtgctactc cgttttctga ttctatcata
      361 cgtaggcatt ttcccttgtc ctcattccaa gtaacataac aataaatata attggattca
      421 tttaagtaca gtaccagtcc agccatctga ttaaaattat taggcatgta ttcaaatgct
      481 gtctccgcat caaaagtaaa atccgtttgc cgaatagcta acaaatgatg ttcgaaggta
      541 gattgaagag attcaccgga aatcagacgt aaaaaacctg gtctacattt tgtatcacac
      601 caatcagcat ttggcaaaat gcgtctggca ttccagttct tctttagctc tccttcaaat
      661 gtatccataa actgatgatc tttttcttgt tgtacctctt catttgtctc aatcattatt
      721 tcttccatcg gagttgtctg gctatcttta agtcttaacc aatcgtcgga cgtccaatac
      781 acttcttgaa tagcggtttc tcttcccaaa atagccgcac cgtttaatgg tctggtacaa
      841 agatatgtca tgtaccatct tcctgacggg gtttgtacta agctcccatg tcctgtacat
      901 tgcaatgtag aagcaggttt gtcactagct gtcagcatag gaaaaccagg atcaagctcg
      961 taaggaccag taatattctt tgctcggcaa actgtaactg aatgtcctct tcctgtaccg
     1021 ccttctgccg tcatcaaata ataatacccg ttacgaaaat acaaatgtgg tgcctccgtt
     1081 ttcgctaatt ctgtacctgc aaatatttta tagatgggac catcaagctt cttcttctct
     1141 gcatcatatt cttgtaatac gataccgctg gatttattcg gtgtgtccag acgataatcc
     1201 catagttcat tgacaagcca tttttttccc gtttcaagat catggaacaa agaaggatca
     1261 aagccgttac tatttaaata aattggttcg gaccaaggac ctgtgatctc agatgcagtg
     1321 atcaaataat tgtggacatc tttaaacgga cggcctgcac ttttgacatc tgtgtaaaca
     1381 agataaaaca atccatcagc ataactgatc tgtggcgccc atatacttcc atttgtagga
     1441 tttccttgca aagatacttg agtggtcaaa atatccgttg tatgtgacca gttgactaaa
     1501 tttttcgact catatactcg gattcctgga agccattcaa aagaggagac aataatgtaa
     1561 taccgctctt tcactcttaa tatattagga tctggattga atcctcttaa aataggattt
     1621 ttcactttga tagtctgcat gattcaggct ccttttctca ttcttactcg atataaactg
     1681 aatgccccag ctgcgaaata aaaaaatgca aaaggaaacg caataaactg agtgccaatc
     1741 aacaatatga atcctgttat aaaatacaaa ccagtggcag ttgttaagta ttttccttta
     1801 gcagtcaaaa acaatgctac tgctaaaaaa gcaagtgaaa taaacaacac aacggcaaac
     1861 agaccagtca tgtcctgtaa taatccaaat gtattttcta aagacaaagt tgaatgatct
     1921 acagcaagcg actgataact ttttttaaac tctgacgcag acatattact taaagtaact
     1981 gctaacccgc caaagaaaat caaagataga ctgccaccga ccattcctag caatagttcc
     2041 cattttcttt tcatttctga accccctgct tttctaaatg tatccattca ggtcttttga
     2101 tcgatgtttc ataattgtta ttacgttcag agaatttgac agtctgttca tttttcttgt
     2161 catcccaatt atcccaacca gttgggtgaa catgcccacc tatcttacac gcttcaaata
     2221 agacatttgc atacggacgc cacggtcttc ctaaatagac gtttctagta tcaggttctg
     2281 cagtcaaggt acagctttca aaacgatacc ctgttttttg atttttcggc gtagatgctg
     2341 ctgtgatgta tccaggattt ttactggcta agctatggat atgacactga tgaaaaacgg
     2401 catctgcccc accaaatatg aaatccaccg ttccttcaat ccagcaatat tcataggtct
     2461 gacgacaata ctcaggattg gcagaagccg gtgtacaaaa aggagtacca tctttttgca
     2521 aatcaggcaa cggacccgtg cataaggtat cctgataacc agatagtcga cagtttttta
     2581 aagatacgcg atctcctgaa ttgaataaag caacagcctg tcctaccttt tctcctggac
     2641 ctgctgtatt ttcgatgtga agattttcta atacgacatt cgatccttct aagaaaaaag
     2701 tagcacttcg gaaagtccct aactggctac cgtcaagata ctcctgtttt gcaaaacgat
     2761 taccggagat aactactgtt cctgctccca caagtttaaa attggataat ctgctttcta
     2821 tatattcatc atatcttcct tcttttacta tcaagacctt tggatggcta tccggcaggc
     2881 ctgccaaata atcgattcct tcttggatag tttcaaaatc cgtcggggta tctatagcat
     2941 tctttccaat tatcagtttg tctttcatcc tcataaatgc tcccttatga attgtttcca
     3001 aatatttctc atttcttgtg tttccatatg accgcctgtt aaagaaaaag aagaaaagtt
     3061 ttctggacta cccattgatt gatacttttt gtccagccct tttctcagct tcagtacgcc
     3121 ttcgtccaag cacatcgtat cgttttttcc aactaagcta agacgagggc gcggagcgat
     3181 tttttcttgg attgctaacg ttgtataagc tgtcaataga tttggcacat agtaataaaa
     3241 cccatgatga tctaggcggc gatgtagcaa aagtatttcg atgtccacct gtgccgctag
     3301 atcaatacaa actttgacac gagaatccaa tgctgtcacc caccagctta ataagcctcc
     3361 catagaaaga ccaatcgttg cgagccgttg agtatctgtg tcttcccttg tctccaaata
     3421 atcgatcaag ctgatcacat cataaatacg cattccccat aacgtttttc ctgttaataa
     3481 aaattgtttg aacaactcgc tttcagaaat acctccgcgt tcttcaaatc cccaagcatc
     3541 aatcgaccaa acagcatatc ccattcctgt cagtacttct gcaaaagatg gagaagctaa
     3601 gtaaggagca ccatggatca attcactttt cccgactgaa aaatctccgc cgtgagaatg
     3661 caggtaaagg accggcggaa acggtccttc tttttctgga taagcataga gggctggtac
     3721 tgtttccaat tcatttaagt acaattgata gtattcaagc ataaaaccgt tttgtttttc
     3781 tcggtataat cgttttccac taggggtatg tctgctagat ggagcaccta aaaaatcatt
     3841 caactgcata agcaaagttt ttacgaacaa ccagttcatc catattttct gattgatttg
     3901 attggaattt ctcaatactg atttctttag tattttctaa tagaaatgct ggtccctcgt
     3961 gattttcaat cgacatctgg ttaaaggaaa ccttacccgc aaagccaata taaaatcctt
     4021 gtctgctcat ttcctctatt cctgtcatca tagcaggaac accgggaact gcatcttggg
     4081 ccatcgagac atcgatattg tcaaatgtga tttcttgtat gaattgttcc gctaatccat
     4141 aaataaaacc tgcagaagca tggacattcc ttgccgtgat attcgaaaaa tggatccttc
     4201 tgaatgctgg tgtccgttca tctatcggat aagccttttt ttcccataca tacggttctt
     4261 tccctcgcgg accacagaaa taataaagat tcagtataaa tggacacatc acattatcca
     4321 tgactatatt ggatactcga atatcttcaa cgatcccgcc tcgacctcgt ctagatttca
     4381 atcgtattcc tcgatcagtt tcttggaaga tacaatttga aatggtaata ttacgaatac
     4441 taccactcat ttcacttcct aatacgacac cgccatgtcc atggaccatc gtgcaatttg
     4501 tgatcgtgat attttcgcaa gcgatccttt cgtacgtatc ttcagtccct gccttgattg
     4561 caatacagtc atctcccacg tcaatatggc aattactaat acgcacattt ttgcatgatt
     4621 ctggatcaat tccgtctgta tttggtgaat ccgctgggtt caagatcgtc aagttatcaa
     4681 acgttgcatt actgcaaaga atcgggttga tcgtccaact tggtgactgg atcaatttaa
     4741 tatctttcac agtaattcga tgacagttat ggaaactcat caatttagga cgaggataag
     4801 cgagattatc tggttcattt ctaaaagtat gccaccattt ttttccattt ccatctaatg
     4861 taccgaaacc agtgacagag atgttctcta cattttgagc atagatacag gaagcataaa
     4921 cttttcgatg gactccttcc caacgtgaat gaacaacagg ataatctttt ggatcgtcag
     4981 agaatttcag aaccgctcct gcgcttaaat gaagttcgac attgcttttt aaaaataaag
     5041 ctcctgttag aaattcacct gctggaacaa ctacggtccc gcctccgtca cttgctgcag
     5101 catcaatggc ttgttgtatt gcttcagtat tcaattcatc aattgatgcg ccaaatgtca
     5161 aaatatcata catatttata atgcctccac ttcaatcaat gcttgcaaga atgcaccaac
     5221 gcctttttga tcattacaaa taattggttc actgatgtag taagcatatg aaccatctcg
     5281 ctgatctgct ccgcctaacc ctgctacttg acagttttta ttcaagttca gccagccttc
     5341 ttttgtttcc agaacaaatt catcaagtaa tccttgatgt gatttcttca cttgtggaag
     5401 ccattcttct ttgttcagca gtcccaaacg tattccttta gccattgcgt aaacaaacat
     5461 actactgccg gaagcttcca gataatttcc tttccgtttt gtttctgttg ttacttgata
     5521 ccatgtcccg ctgttttctt cttgatatgg aagtaatgca gccagtgtct tttctagaat
     5581 ttctttcagc aaagaacaat ctgtttcttc atgaagtaat tcataggtat ctgctgcagc
     5641 catcaaatac cagccaatcg atcggctcca aaaatgatgg gatagacctg tttttggatc
     5701 agcccatggt tggaccttct tttcatccca tgcatgaatc agcaaatttg tttttggatc
     5761 gaatagatgt ttataactga tttcaaactg tctcaaaaca tcttggtaat ctttttcttc
     5821 actaaaattc tggataaatt cagcataaaa aggagaaccc atatataagc catccaacca
     5881 aatttgataa ggataaattt ctttatgcca aaaagctcct tcggatgtgc gtggatggtt
     5941 tgccaactgc tgggaaagga gttctgctgc ttttttatat ttttcttcgc ctgtttcttt
     6001 gtacaataaa aagaaaagct ttcctgtatt tacatggtcg atattgtatt cttcaacgct
     6061 gtagccacgg atcgttccat cttcttgcac gaaatagtcg atattgtctt ttatgaaatc
     6121 aaaataaatt ttttcttggg ttcgttccca aagtaattgg aaccctttaa gtacgacgcc
     6181 atagtcataa gaccattttc ctttatacca cttttcttca tataagcgag gtgttctttc
     6241 catgatactt tttgctaatt tctttgacca gttttccatt atttgctctc ctttaaaaat
     6301 taaaatagga tttgggacaa ataccaagaa gtatttgtcc caatcgtcta tattactgtg
     6361 ccgctgcttt gtatgcttcc tcgtactctt gtgtgattcg atcgcctcct tgtgctttcc
     6421 agtcttctac agcttttttg aatccatctt cgtcaagctg tcccatgatg tattgcaccg
     6481 tagcatcaat gacgattttt tcaatttctg atcctacttc attgttcgtc ggtgaatcca
     6541 atggcacaga aggatccatt acagcaaatt cttcattttc tttgatcaat tgattagaga
     6601 attctttttc agggtctgta tctttgaaag agaacgtcac ttcactagga cgagagcttg
     6661 ataacggttg cacctcttgt tgccaaagat ccatatcgat aaattcgata gaaccatcat
     6721 cattcaattc ataatgcgtt ccttcgatac cgccagtcat caatttatac acatcttctt
     6781 ctaaaagatc atcaacaaat tgaagtaatt tcttcaattg tgcttcgtct tttacttgag
     6841 atttgggaaa tgctagcaag ccgccaatac catttccttc tgaccaaaca tgataaccgc
     6901 cgccttcacc atctgagatc ttgtttacag gcactaattc catatcatct tgaatacctt
     6961 gacccatcgt cttcagattc ttgatatcca ccattccagt gtagattcca gttcttcctt
     7021 gtgcaaattg ttcttgctga tcagtctttt gggtaactgc aaagtcttgt gccaaatagc
     7081 cattctcaaa tagatctctt gaaaaattca aagctttcat atactcttct gtatcaaatt
     7141 ctggagtgaa tttgccatta tcgtctacag cccagccatt tggtgctcca aaataagaag
     7201 tgaaaagttt aaagctggta taacgaatat cactaggcgc acgatcgcca aaaccaactg
     7261 tgtcgtccac accatttcca tcaggatcgt tttctgtaaa ttgtttagct acttcataaa
     7321 gctcatccat ggtagttgga acatctagtc ctagattatc caaccagtct tttcgaatga
     7381 ctaagccagc acgagcgtag tctttttgta tcggtacgcc gtacaaggca ccgtcgatac
     7441 tcgcagcttc gattctatct tcagaaattt gacttaaatt tttatagtct tttaaatatt
     7501 ctgatacatc ccagaatacg cctgatttta acgcattgcg gacagaagag tttttcaaca
     7561 tcgcaatttg aagtgtaacg atatctccta gttcatcaga agccaatgca gttgttaatc
     7621 tttcttcttt tgaagcatca ggaatccagt tgaatgtcag ttccgtatcg gttttttctt
     7681 cgatcaagtt cacaatcgtg tctgtaggcg gtgaagcatg atgcaaaata tttaaccaag
     7741 tgattttagc ttttccatct tctgtctctg aagaactgtt acttgaactg ctgccgcatg
     7801 cggcaagtgc tagaattcct gcaaaactaa gacctaattt tacgagcgtt gcttttttca
     7861 taaattttct ccttcactgt tttaataata tttttgtttt cttctgtttt ttctgtatat
     7921 gagttcttcc attccaaatg gcaaaatata gaatttgctg tccacgtgac tgcggaaaag
     7981 aagagactgc caccgataaa ggttaaaaca cctggcattg tttggatgat catcagataa
     8041 atccctacca tacttaatat caacactaat gagtaatgaa gatgtgagaa tccgaataaa
     8101 aatgccattt tcattttgaa aaaatatcct ttccaatcat aatgagccat gattgggaaa
     8161 atatagagtg ctgagagcac atatatgaac gcagcaatat acaaagcgat cctaagatac
     8221 caattgtgta tgaaagaaag atccacaagt aaaacaatcc cgatggcgcc atatatccag
     8281 ctcatcagca aggtttcacg atagttttct ttaaaatatg tgaagtacgt tttcgtcaaa
     8341 ggaaacgttt cttttgagcg cagccatttt ctaatcactg caaccatcgc atacgatgca
     8401 ggaccgaaag taaaaactcc caaacctgcg atgactaaac cgccccacag aaaattcaaa
     8461 taaaccaact gtaaaatacg atttagcctt ccgtttattt tatctaatga ctgcagcata
     8521 tctttccctc tctttctatc ttagtaaacg ccgtcttctc ctagcttttt ggcaatctta
     8581 ttagctaaaa ttaccataaa caatcctacg acccctttaa acaatcccat tgctgtactg
     8641 aaacttaact gtccattctt caatccagct gtataaatat aggtgtcgaa aatttctgca
     8701 acgcttcgat tcaatgaatt caataacaag tacatatgct cgaaccctaa gtctaatgtc
     8761 tgaccgattt ttagaatcag caaggtaatg attactggac gaattgccgg caatgtaaca
     8821 tgccatgttt tgcgtaagcg tcctgctcca tccatttctg cagcttcata caactgtgta
     8881 tcgactgcag ttatcgctga aaggtagatg atcgtagacc agcccaactc tttccagatc
     8941 acttgtccga tatatactgt gcgtgtccat tcaggggatg ttagaaaact gattttttca
     9001 aatcccattg ttgcaaggaa ttcattcaat acccctccgt caacattcaa aaatacataa
     9061 gtaatcgaaa caatgattac ccaagacatg aagtgaggga tgtaaatgat cgtttgaatc
     9121 ccgtttttca atcgtttgtt cgttacttca tttaatagta aagacaaaat gattggcatc
     9181 ggaaaaaaca cgaagatatt taatccgaac aagatcaacg tatttcttaa taatgtaaag
     9241 aatgtcggtt ctgtaaacaa acgaatgaaa tgtttgaaac cgacccaagg acttcccatt
     9301 attcctaaaa atggttgata atcttgaaaa gcaataacga gtccccccat aggaagatac
     9361 ctaaaaatca agaaataaag gactcccggc agaatcatca catataaaaa tttattcgtt
     9421 ttgattcctg caattatttt ttttcgtctt ctgagctttt cttctttttt cgatagcgtc
     9481 agttgaaatg gttcttgttt ctttacactt tccataagcc cctccttagt tttgtgaatg
     9541 tttgctgctt atgacttcat gatagataac tatctgaatt acgtatataa tcatttttac
     9601 cgtaagtaaa aacgcttaca tagcaatgat ttttgtgcta tacaaaacga taaagtgtat
     9661 tttttcacaa ataaactacg catagcaaac cttctatttt tttctatctg acacttagca
     9721 taggaaatga ttatatacgt aatctttcac cgatgataaa ttgaacatat agaaacagaa
     9781 acatttaatt tattttgagg aggagcctat ctatatgaaa aagaaagtct ctataggaga
     9841 aagaagtttt acacttttca acaatacgtt tctaattttg ctagctttga tctgtatcgt
     9901 tcctttttta aatatcatcg ctacatcttt tgcttctaca caagaagtcg tcgcgaaaaa
     9961 gtttatcctt tttcctacta ccttttcttt agacgcgtat cgctacattc tttctacgcc
    10021 gaccattttt agagcacttg ctgtttcgat tggtgttaca ggtgtcggaa cgatcgtcag
    10081 tatgtgtgca acttcactga tggcctacgg tctttccaga aagtatttgt ttggtcgagg
    10141 tttcgtcaac tttcttgttg tattttctat gttgttcagc ggggggatga ttccaacgtt
    10201 cttagttgtt cgttctctag ggctggtcaa ttcttactgg gccatgatcc tgcctgtggc
    10261 tgttaatgca atgaatatga tcattatgag aaactttttc caagccttgc cggatagttt
    10321 ggaagaatca gcaaaaatgg atggttgcac agattttggt gtatttttca aaattatgct
    10381 gccattagcg ttaccgtcta ttgctactat ctcgcttttt tatgctgtta cttactggaa
    10441 cacttatatg actgctattt tgtatatcaa cgattcatct aaatggccga tacagattct
    10501 tttacgccaa atcgtcattg tatcaagtgg gatgcaagca gaaagtagtg ccgttgatgt
    10561 gattccacca gcacaaacag ttaaaatggc tgttatcgtt attgcaacag tgcctatgct
    10621 gattgtttat ccttttgttc agaaatattt tgtcaaagga gcactcgtag gttctgtcaa
    10681 agggtgatta tgtactgaac gcaaaatggt agttgtgtca gaagtggtca gcttcaagaa
    10741 ataagaaggc atttacgaaa attgcttctt aaatttttgt gaaatttcgg cttatttttc
    10801 gaagaatcta ctcctggaac accgtgtatc agaggttgtg aaagagacgt tcagccttga
    10861 gcgatgataa aaactctcac aaacattgcg acgagtaacc cacaggagca ataagaagcg
    10921 attttgaaaa atagcttccc atatttcaca aaattatgcc ttattgtcga tagcctgtct
    10981 cttgaacgcc atttattcgt aaaaaagaac tgtgacaaga cttttgtcac agttcttttt
    11041 gcgtgcttag ccttgaaatt taactatcca taaatacaaa tactagggtt attctaactt
    11101 cttttattgc ttgaatcagt ccacaagaaa aacagcctct tgattcacaa atattttttt
    11161 cgatattctc ccggcgtaat cgattccttc tttttaaaga aacgaataaa attttgcgga
    11221 tttcgatatt gcaatctttc tgcaatctct tttacggaga ggttagtctc tttcaaccat
    11281 ttttttgcta cctctaaacg atgattttga acaaaatcac caaatttttc tcctgtttcc
    11341 tttttaaata cattacttaa atagttaggg ttataatgca acctatcagc gatcacatct
    11401 aaagacagat cacgatcata ttctgtgtag acgatatgca taatattctc cgacaaattt
    11461 ttaaattcat gatcagtgat tgcttgagag cattctgtga ttggcagaac taattgttct
    11521 aaaacaagtt tttctagttc tttgggatga taattgttca gtgcttttac ataaatttgt
    11581 ttcatgttat caaagttttt tgaatctact cccaataact gtcctaattg aacgagttca
    11641 ttgatcaagc ggaccgaaac tacttcccga ctcaaaggat ttttattttg tgcaaacaat
    11701 gcatcaatca attcatgtac taaattcttc acttcctgat ttccagaacg aatggcttca
    11761 aacaatcgat tttgttcttc tatcggatat ctaataagtg ttttttcatg agcagcagga
    11821 acgatttcct gataaaaaat aattgaactt ggaccagtat ttacacgata atacagggct
    11881 tcttttgccc gatcaaccga ttgcttgctt tcattcagcg tttcgaaccg gttactgatg
    11941 cctatactga tagttaaatt caagtattct tttacacgtt tttgaatgag tcggcaatac
    12001 tccatgactt ttttgcttgc ttctgcatca tctttatcca ttcgataaat agtggcttgc
    12061 atttcttcat tcaaaacgat cggcagcatt cgattttcct ttggcacaat ttccgtgatc
    12121 atgttgttga tcgctaaaag tagcaaatca cgctccccac cgtgactatc ttctaacaga
    12181 tcgatttgaa ccaaagctgt ataatagcag tcattttgag aactataacc aaattgctgc
    12241 aaccgctgat ctagttctcg cctttctact cgatttctaa ataaactaag tacaaatagc
    12301 gtttccagct gtggcttttg ggtaactaag tgtgcactta acgcttcttt ttctcctatg
    12361 atccgactga ctgattgagc gaccaaatct agttctttcc cactgaatcc ttttgattta
    12421 agattcagac gttcctgtat ttgagaaatc ggacgggcaa atctgtccga aaataagtag
    12481 gaaagaagac caatgagtcc taccagtgtt aaagacactg caactagccc gattctcaga
    12541 ttgcggatca ttgcgccaat tgcactagag tcaacttcta atctgtaaat ccaattatta
    12601 taatctgatt tcagtactat atacgtttta tctttttctc ttatcacttt cagctgattg
    12661 taatctagat ccacgtccgt taatacagtt ggttgctctt tattttttac tgtctgagta
    12721 gtaaaaaggt tgccaacttc gttttcaata gaaagtatgt cttctccact ttcaccaaca
    12781 acttgttcta ttgtatgttt ctctatcgta gcaattccca atgcatattt ttctttttca
    12841 aacattggca aagaaatcac catttcgatt cctttgtttt tcttttgcca aaataaatta
    12901 tttttgttat ttatgtactt ctgttgatac ttttcaattt cctcttcggt cagttgactc
    12961 aacgatccat tgattactcc ccatttacca gccaaactga ctaaatcata attattatca
    13021 tccataacaa tcgtttctat aaaatttagt tctttcctga tttcagaata agtggagtaa
    13081 tctgtatatg tcagcggatg cttgaatatg tccgagaaat ttttggtcga accatacgta
    13141 gaaaacgaat attcaatcgt tctaattttc cattcaacgt tagttttaac ttgcctgagc
    13201 aaattctttt tgtctgttaa ataactcgat tcgatcgatt cataggtatt ctggtaaatg
    13261 aataaactaa atcctatgac aagtacggct cctaataaaa acatcggtaa aaatatttta
    13321 taaaatactg tacttttctt cattttcttc ctctcctcct tttaaaataa aaggctttca
    13381 tttttctgta aaattgatac tcatctaatt aaatgagcat tccccgcttt caagaatagc
    13441 ataattaaaa ataaaaatcc aagtaatttt taacgtgtgc aatcttttca tatcaaagat
    13501 ctattgactt atcgtagtta tcgtaatatg atgaatgcgt taacaaaaag tgaaaaagta
    13561 aggaaaatga tggaatggta acgatcaaag atgtcgctaa agcagctggc gtttctcata
    13621 cgacagtttc tcgtgctttg aataacagca gtttaataaa aaatgaaaca aaacaaaaaa
    13681 tacgacaact agctaaggaa atgaattatt cgccaaacta tagtgcaaaa ggtttagtga
    13741 atcaaaaaaa atatctaatc ggactatttt tctcaagttt gcataaagga acttcatcaa
    13801 atttcctagc agattccatt gcaggcatcc attcagtatt agacacaaat tacagtttat
    13861 ctgttgaaag tatcgatgtt gttgatttaa cagaaatcaa cctgcaacgc tacgatggac
    13921 tgatcgttat gagtcaaagt gatgatgatc agcctttcat cgactatctc aaagaaaaag
    13981 aatttccttt cgttgttgtc aatcgtcaca tcaatgatcc agcggttatc aacgtcactg
    14041 ccgatgatgc ggccggtgtt atgcaggcag tagattacgg tattggatgc ggacatacga
    14101 agatcgctta tattggcgga aagaaaaatt ttcgttcgac aaaagaacgg gaaaaagggg
    14161 tattgaaaag tctaaaaaaa gcgagcatcc ctgtcaatcc agcctatttt ttaacaggag
    14221 attacagctt agaaagcggc ctccaaaaca tggaatacct attatcactt tctgacctgc
    14281 ctactctggt tttttgcggc aacgacgaca tagcgattgg tgcattgaga gctgtacatt
    14341 tagctggcct ccgtgttccc cagaacattt ctctgatcgg gttcgatgat tctccagtcg
    14401 tctcctatct caatccgccg cttaccaccg tacgtaagcc tttgaaagaa atgtgtcaag
    14461 aaggaacaaa gcttttacta gatttgatcg aaggcaggac gatcgaacaa tctaaaattg
    14521 agctctccac cttgttgatc cttcgtgagt cgatcaatta cttgtaaact acaagtaatt
    14581 ttatataaaa atgttaacgt gtgcataaca cagaaaggaa gtttatatga aaaaactgac
    14641 aaaagaatta gtgaaaaaaa acgaagcacc tgtaaaagtt cttcagtttg gtgaaggcaa
    14701 ttttatgcgt ggatttattg attggcagat tcagcagctg aataaccaac aattgtttaa
    14761 aggtaatgtt gcaatcattc aacctttaga acaaggtctt ggaaatatga tgaaccagca
    14821 agataattta tatacggtca ttttacaagg actcagcaat ggcgaaatcg ttgatacttc
    14881 cgagattatc accgtagtag aacaaatgat caatccttat gatgaatggg atgcttatct
    14941 atctctagca gaaaatgacg acttggccta tatcgtctcc aatacaacag aggcaggaat
    15001 ccaattcgtg gagactgata atttaacaga tactccccca cagagcctcc cgggcaaact
    15061 gacagccttt ttataccgac gtttcaaatt aaacaaagct ggctttacca tcattccttg
    15121 cgaactgatt gacagaaacg gcgaacgtct aaaagaaatc attttacgct acgcagaaaa
    15181 atgggattta gaacctgctt ttgttgagtg gttagaaaaa gaaaatgttt tctgttgtag
    15241 tttagttgat cgcatcgtcc caggttatcc tcgcgaagat gcaggtgttt tcaatcaacg
    15301 gcataactat gaagatcaat tgatggtcaa agcagaacca ttcatgcttt gggtgatcga
    15361 agggcctaaa gaattggaga atcaactacc attcaaacag gctggcttga atgtcatcat
    15421 cacagatgat atgactcctt atcgtgaacg taaggtgcat ttgcttaatg gaccgcatac
    15481 agcgatggtc cctcttgctt tgttggcgga attggaaact gtagaagaag taatgaaaga
    15541 tcctttattc tccgcatatg ttgatcagtt attcaatcta gaattgattc ctatgctttc
    15601 tttacctaaa gatgaattag ccatttatgc tgaccaaatc aaagaacgtt tcttgaatcc
    15661 ttttgcccat cacaaattag aagcgatttc attaaatagt gtttcaaagt tcagcacccg
    15721 tctgcttcca gtgttcaaga agtatatcga agaacaaaat caggtaccac cgttaatcac
    15781 tgtttcacta gctgcgctac ttcttatgta tcgaggcgat caagtaaagc cacatgatga
    15841 tgaaaagacg ataagcgaat ttactgatgc ttggtctgat aatggaacag ctattcctcg
    15901 attattgcaa aacgctgcgc tttggggaga agatttgtct caaataccga atgtgacaga
    15961 tacagttcaa gaaatagctg caaagatcga tcaaaaaggt gtccggtcag tattacaaga
    16021 aatgaaagga acgatggtcc atgcttgaaa ccctgcaacc aacaatgaaa ttaaatcgac
    16081 gagacagtgt ggtcgtcgca ttaaccccta ttccaaaaca aacacaactg acgattgatc
    16141 agcaaacgat caccactctt gaagatatcc cgcaaggtca taagattgca ttgggtgatt
    16201 tgaaagctgg agacaacgtg atcaaatatg gctatccaat cggtcatgtg acgacagacg
    16261 taactgccgg tcaatggctg cacactcata atgtaaaaac caatctttct ggtgaattgg
    16321 attatcgata tgaaaaagat gtccatccaa gtcattacct ttttgagaac cgaacatttc
    16381 aaggttactt gcgaaaaaat ggaaaagtag ggattcgtaa tgatttgttt ttagtaccta
    16441 ccgttgggtg tgtgaatggc attgctgaac tgatcgtcaa acagttcaag gaaaaacatc
    16501 cagatctggg acagtttgac catattacga ttttaaagca tccttatggc tgttcgcagc
    16561 ttggaaaaga tcatcaaaat actcgagaaa ttttagcgga cgcagtaaat catcccaatg
    16621 ccggaggagt tttagtattt ggattaggtt gtgagaacaa tacagttcct gaattcaaaa
    16681 aaattttagg tgaatatgat gaagaacgag tgaaatttct agtggctcaa gatgtttacg
    16741 atgagatcga acaaggtgtg gctctattag aagagctttt aacagcggcg gaaaatgacc
    16801 agcgcatatc agtcccgctc tctaagttga atgtcggttt gaaatgcggc ggttctgatg
    16861 gtctatcggg aataactgct aatcctttac ttggtgcatt ttctgactac ttgattgctc
    16921 aaggaggcag cacgatctta acagaagtgc cagaaatgtt tggcgcagaa caagtattga
    16981 tggcacgagc ggaaaatgaa gaagtttttg actcaatcgt tgacttgatc aatgacttca
    17041 aacagtattt cttgtcttac ggggaaccag tttacgaaaa cccatctcct ggtaataaag
    17101 caggtgggat cactacatta gaagataagt ctttaggctg cacgcaaaaa tcaggaagct
    17161 ctcctgtcgt tgatgtgcta caatatggcg aaaaaatcag aaaaccaggg cttagtttat
    17221 tgcaagctcc cgggaatgat cttgtagctg cctctgctct tgcttcatcc gattgccagt
    17281 tggtcttgtt taccacagga agagggactc cgtttggctc atatgtccca acggtcaagg
    17341 tatctacgaa tacaactctt tttgaacgca aaggtcattg gatggatttt aatgcaggcg
    17401 tcttattaga acaaccaatg gaagtaatct tagaatcgtt tgttcaaaaa atcatcgctg
    17461 tagctagtgg agaagaaacg aaaaatgaac aaaatgacgt tcgagaaatt gccattttta
    17521 aaaacggagt aacactttga ttttcataaa acagtataaa aagaaagaag gaagatttat
    17581 gtttttatct gatgattttt tattaaaaga cgattgggcg aaaaagcttt accattccta
    17641 cgcaaaaaac atgcccatta ttgattatca ctgtcactta gatccaaaag aaatttacga
    17701 aaacaacaat ttcagcaatc tgacagaagc ttggttagga ggcgatcatt acaaatggcg
    17761 cttgatgcga gcttgcggcg tgcctgaaga aaaaatcaca ggaaatgctt ctgattttga
    17821 aaaattttta gcttggtgtc aaaccgtacc aaaaattatc ggaaatccgc tttacagttg
    17881 gtcacatctt gaattgaagc gtttcttcgg tattgacttg ttgataaatg aagaaaatgc
    17941 agagaaaatt tggacacaag caaatgaaat tttggcagct cctgattttc gccgtcggga
    18001 aatcgcaaaa aaatcgaatg tagaagtgat ttgtacaaca gatgatcccg cagacgactt
    18061 gcactatcat cagttgcttg ctcatgaaga accagatttg aaggttctac cttcattccg
    18121 tccagataaa gcactcaata ttgaaaaaga aggatttaat gcatgggtca cactactgga
    18181 agaagcagct gaccaaacga tcaccaccta tcaagaactg atagaagtct tagggcagcg
    18241 gatagaatac ttccatcaac atggatgtcg tgtttccgac cacgctttag acatcttacg
    18301 ctataaagaa gcggatgaaa cagtattaga agagattttt caaaaagcac gagctaacaa
    18361 actattatct tcagatgaaa tcgatgctta tcgtacagaa acactgatac atttgaccca
    18421 cttctatcat tctcataatt ggacgatgca gctgcacatc catgcttatc gtaactgtaa
    18481 tacacaaatg ttggaaaaat tagggcccga taccggctat gatggaatga atgatctttc
    18541 tttgactatt cctctgcaaa aattattgaa ccgtgcagaa gaaacggatc agttgccaaa
    18601 gacgatcctt tattcattga atccgaatga ctatcctgcg attttagctt tgatgggctg
    18661 tttccaaaaa gaacagaatg gaaaactgca gttaggttct ggatggtggt acaacgatac
    18721 tcgagctggt atgcgcgatc aattgacaca atttgctgat ggtagtgctt tggggaattt
    18781 tgtcggcatg ctgacagatt caagaagttt cctgtcctac actagacatg aatatttccg
    18841 tcgtgttctt tgtgaatttt taggcgaaat ggtcatgcga ggagaagcac ctgaagatga
    18901 acaactttta ggaagtctag ttcaagcgat tagttataca aatgctaaaa attactttgg
    18961 cttttttgcc taattgatta atagtaacag taacgaatca aaaacaggat agtgaaataa
    19021 cggaatccgt ttatccacgt tttagagaag aagacggacc aaatttgctc cgtcttcttc
    19081 tcttgctgtt ctgcttcatt gcctataaac ctttatgaaa gaagagataa catgcctact
    19141 atccatattg ccggtgattc tactgcggca gaaaaagatg aaaacaagcg tcccgagaca
    19201 ggttggggag aaaaaatctc tgcttatttt tcacccgaga tccagatcaa taaccaagca
    19261 aagaatggac gcagcaccaa atcatttatc caggaaggaa ggttagcccg tctagcttca
    19321 cagtttcagc cgaatgattt cttatttgta caatttggac ataacgacca gaaaatccaa
    19381 gaagaaagag gtacgcttcc cttcggtagc tatttaaaaa atctgcagat attttttgaa
    19441 tgcgcaaaaa aagcgaacgt ccaaatggtc ttactgacat ctgtgacgcg acgagattac
    19501 ttagaaaacg gaacattgaa tccagatata cttggagact atccaaaagc gatgcgtgca
    19561 tttgctgaga aacaccatat tcctctttta gatgtgttct caagatcaca agaattgttc
    19621 cagaccttct caaaagaaga gacaaaaaaa ttctacctgc atcttatacc tgatacttgt
    19681 aaaaattacc cagaaggact aaaagacaat acccacttta gtccagaagg cgctgataaa
    19741 gttgcccgaa taattgttga attgataaaa gaatcccgtc tgcctcttgc taattattta
    19801 caaaaaggag tttgagatgt ttccattaaa tttaggcatc cgtgcccacg atttagatgt
    19861 tcgaaatcgt gaagaaatcg ttcaaaaagt atcacagctt cacttgtccc atatccaatt
    19921 tgcaccacaa aaagcatttc ctgaacaact taaattaact gacatgacac ttgggagtgc
    19981 tgcctatttt ggtgaatact tccaaaaaaa caatatcgaa ctttccattt taggatgcta
    20041 tatcaaccta tccagcaaaa atcaagaagt tcgtgcacaa gcattggcaa ctttcaaaca
    20101 gcaaattgct ctgtgctctg cctatcaagc caagatgatt gctacggaaa ccggtagtgt
    20161 ctctgtcggt tacacaaaag aaaattttac tgaagaagct tatcagatcg ccagaaattc
    20221 tatcattgaa ttagtcgctc atgcagaaaa ttttggcgta actgttgctg ttgaagcagg
    20281 gatcaatcat ccaatctata actatcagtt agctaaacgt ttaatcgatg aagtagcttc
    20341 accaaattta aaaatcattt tagattgtgc gaatttgatg cataaagaaa atcatgctga
    20401 tcaggaaaaa gtgatccgag gtgccttgga caatttacat ggttatatta cagcgcttca
    20461 tctcaaagac tatatcatgg aaaatggaaa gatccgcatg gtacctgttg gcaagggctg
    20521 tttagatttc cgcccggtac tgcattatat caaatatgag cgtcctctaa tgtacgccac
    20581 tcttgaagct actcctgaga tttatgttcc tgaagcaata gaacacttgc agtttattta
    20641 ccaagattgt taaggagaag attatggagg aacgaataca gaaaagttat caaaaatatc
    20701 atgcaaatgc ttttcagaca ttgctgcatc tttatcaggg aaattattgg cgttttgcca
    20761 tgtcaggact cttttttatt atcaaacatc ttccatcttg gttaatgccg atcgctattg
    20821 ccaatgtaat caatgcagct tcatcgaaag attcttctca gttgcaggcc atttatttca
    20881 acgctttttt gatggcttgt ctgattggtc aaaatatatt gacgaattat tttcatgtaa
    20941 aataccatag cacttcgatc cgtcaagtag aagctggact aagagcgacc cttatcaaga
    21001 aaatccaaga actatctatt ccttatcaaa aagaattgca atctggacga atccagtcaa
    21061 agatcatccg tgatgtagaa gctatccaaa cgttgtcctc tcaagtattt gtcagcggat
    21121 taaatatcgc tgtcaattta ttcgtagctt taggtatcac gctttataaa agtcctgttg
    21181 ttttcatgtt tttcttattg actgttcctc tcgccgcatt cgttactctc tttttccgta
    21241 aaaggatcag acagtccaac tcagacttca ggcaagaaat ggaagagact tctgcaaaag
    21301 tgatcgaagt gatcgaatta gcaccgattg ctcgagctca tgggttagaa aaacacgaaa
    21361 caactcgctt aaataagcat tttactcaaa tctatgaaaa aggattccac ttagatatcc
    21421 tgcaatctgt tttcggatca gtcagctggg ctagttttca gatatttcag ctgatttgtt
    21481 tgattttcac tgggatatta gctttacaag ggcgcatcca acccggcgat gtcgtgatgt
    21541 atcagacgta tttcacgacg atcgtcaatt ccgtttctgg aatgatcacg ctgattccta
    21601 cgatttcaaa aggcatggaa tctatcactt ctattggcga actgttgatt gctggcgatg
    21661 tagaaactta tcacggtaag aaaaaaatcc aacatttgga tggaaatttt cgttttgagg
    21721 atgtttcttt tctttatccg gactcactca aaccgattat cgatcatttt tctctagagg
    21781 tgaaagctgg agaaacaatt gcttttgttg ggccatctgg ctctggaaaa tctacactcc
    21841 ttaacttatt gattggattt atccagccga cttcaggaaa aatttttcta gatgatcagc
    21901 ctttggatga actggatatg cgaagtgtgc gaaattattt agccgttgtt cctcaaacca
    21961 ctttgttgtt ctccgcttcg atcaaagaga acatcactta tggcttgaaa aacgtatcaa
    22021 aagaacgttt agatgaagtc atcgaagctg cgcaactttc ttcactgatt gaccagcttc
    22081 ctgatggtct ggatacctta gtaggcgaac atggcaacaa gctgtctggt ggccaaaagc
    22141 aacgtatctc cattgcacga gcgttgatac gtcagccaaa aatcattttg ctagatgaag
    22201 caacctcagc tttagataac cagtcagaga aaaaaatcca gcaagccctg aattacctga
    22261 cagaaacacc cactactttt attgtggctc atcggctttc aacgatcaaa gaggcagata
    22321 aaatcgttgt gatcgatgaa ggaaaaatcg ttgaaatagg tacttacgaa aaactgttag
    22381 ctcaaaaagg ctatttctac cgcttgaaaa atgaatgaca aaaaatagga gctatgacaa
    22441 gacttttgtc gtagctccta tttctgaata aacggtgttc tcctgccaac tcctcggtca
    22501 caagtcacaa tagtcatcaa acatgagaag ctgttttttg aaattgttcc ttgtaactca
    22561 gagttaaacg gtaggctcac gacctcttgg taaatggtgt tcaagagaca gcctatcggc
    22621 aataagaagc aattttgtca tcgctcaagg ccgaacgtct ctttcacacc ctctgttgtt
    22681 tcataaaata gttttattcg ctgtaaaggt cgcgaactgg tgtcatgatg atacggttca
    22741 agtcattgat gattgtactg aagccttgct ctttttgcat caagtcattg attacttctt
    22801 ctgattggat tttcacagcc atttcttgtg ctttcattgc atcttcgtcg ctgaactctt
    22861 gtccactcat ttgtttttct tgtaattcga tttgcatcgc ttggaagtct ttgaacagtt
    22921 tgtgtgcttc ttcgtttgct tgtactttct cgtacgcttc tttcaacgct ttgaattctg
    22981 gtaattcacg gatctcacgt tcgatttgat tcgcgctatc gtagatgttt gacatgttat
    23041 gccctctttt cttttagtag ttactctcta ttgtaacatg gggttattcg ttttcaaagg
    23101 ggctactctc cttgatattt ccgccagagg tctttagccg cttcaccaat tccctgaacg
    23161 ccttctttca ccatatcgcc gaagtttttc agcccttctt ttgcttgttc accatattga
    23221 ttcaagttat cttgccaatt tccagactct ccattagaat ttcctgtgtt tccaacttga
    23281 tctgcagcta ccacttgtcc tcctgtggca taggcatctg ctacactaaa tcctgttttt
    23341 gaagtatacg gaaggatgcc ttctgccgct gatttgaaga ctttcccgac aacatttcca
    23401 cttgtccctt ccaaatagtg cagcttgctg gattcttcgt aacccatcca tgtcgaaatc
    23461 actacgtctg gcgtgtaacc gatcatccat tggtcatttg cttttgtggc atcgaaatta
    23521 gtttctgtgg ttcctgtttt ccctgctatc gtataacctg caggattcgc agcagccgct
    23581 gttccatttg aaaatgtacc aagaagcatg ctggtcattt catcggctgt ttcctttgat
    23641 atgacctgtt ttggtttttt gtttgtgtta tcgacgatca ccgctccagt cgaatcaacg
    23701 attttcgtga taagatgcgg cgtatacatc tgaccatcat tggcaaatgc actgtaagca
    23761 cccgccatga tcaatggaga tactcctttt tccagtccac caagggctaa gccataatac
    23821 ttatcagatt ttgctaaagg caggccgaat tcttctgctt tatcatatcc tttttgtaaa
    23881 ccgatctcat gcaaaagcca tacagtaggc aaattcaaac tttgtgcaac agcttgatac
    23941 atcggtactt ctccttggta tgtcccgtca aaattatgcg catcgtaata actttgcggt
    24001 tcgtctttca agatactgga aggcttgtat ccagcttcca gtgctggtgt atatacgctt
    24061 aatggtttga tcgttgatcc tggtgaacga cgcatctgtg tagcaaagct gaatcctcta
    24121 aagacatgat ctcctcttcg tcctactagt gcttgtacac cacctgtatt cggatctaaa
    24181 gctactgaag cagattggac catggcacca tctgaagcat ttgctgggaa taatgcactg
    24241 ttttgataag tagcttctaa ttgcttttga taatcctgat ctaaagctgt atagatttta
    24301 tagcctttat tcatgacatc ttcttcatca agaccatact cgttaactgc ttcatcgatc
    24361 actgcatcaa aataataagg ataacgataa ccagaatctg aattatcgta tgtgtcatta
    24421 agatacgcgc tgatatctgt ctggctttct gcttgttctt gttcttttgt gatttttccg
    24481 ttttcttcca ttacacttag tactgtgttt ttccgatcat tcgcattttt tgggtgatcg
    24541 atcggattat aaataccagg acctttcagc attcctgcca aagtagcagc ctcagacagc
    24601 gtaacattcg ttgcatctac tccaaaatac tttctggaag cgtcttggac accccatacc
    24661 ccgttgccaa aataggcatt gttaagatac atggtcaaga tttcttcttt gctgtacttc
    24721 ttttcgatct cgattgccaa aaataattcc ttcgcttttc gatcaaacgt ttggtctaat
    24781 gtcagatacg catttttggc aagctgctga gtgatcgtac ttcctccgcc ccctgatgtt
    24841 ccgccagaca agaccattcg gactgctgca cgggcaatcc ctttgatatc aaacccgtga
    24901 tgctcgtaaa aattcctatc ttctgtcgag attactgcat cttggatata gggagaaatc
    24961 gcgtttaatt ctacatatgt tcctttttgg gcgtacagtg tgccagcttc ttcgtttttc
    25021 ttatcataaa tgactgtcga ttggcttaat cctgatttta acgtttcgac atttgcttgt
    25081 tttgctaaaa taaagaggta gatacttgtc actaagacac agatcaaacc aatcagcaaa
    25141 atcaatttgt tcacatgata ttttttccag atgcgctttc gccatcgatg gaattgtccg
    25201 agatatggtc tgatccaacg ccaaaaactg atcaaagcag cttttatctt ttctaaaatg
    25261 ctttgaaaat ccatttgctt cttctcctgt ctatttgatt caatcgtctt attttaccat
    25321 agtgaaagag tgttttcttc ctgcttaagt tgtagttcaa tatgtttttt tacgttagtg
    25381 caagtagagt aacataagaa tgaacagaaa aatgtaagtt ttttcttaaa tatgttttaa
    25441 aaatggtaca attagtctgc gattgcactg aaaggagtac acttgtgaaa atcacgatca
    25501 ctttacctat gaaccatcct gaaacaacaa ttcgcgactt gcttgaaaag gaatggttag
    25561 ttcctagaaa ggttcgacac tttttgcgta tacgtaaaaa cgtttggatc aatcaacaac
    25621 ctgctttgtt ccatcatgaa gtaaagggca atgaccagat cacattgtta ttcgaagaaa
    25681 cagattataa ttatcaagaa gttcaattag gagatgcgaa gcacatccgt gttttatatg
    25741 aagatgagca tttgattata gtggataagc ctgcaggaat gaagactcat ccaaatgaac
    25801 cgacagaaaa cagtacctta ctcaatcatt tagctgctta tctcgacact aaacagcaac
    25861 gaccttacgt tgtccaccgc ttggacaaag aaacaagcgg tgccgtttta tttgcaaaaa
    25921 atccatttgt cttgccgata cttggaagaa tgttggaaca aaaaatgatc tatcggagat
    25981 atcaagccat cgtatgggga gaaattgcca aagatatgac catcaccaat aaaattggac
    26041 gagatcgcca tgatcggaga aaaagggtcg tagatgataa aaatggtcag agcgccttga
    26101 cacatgtcac tgtcaacaaa gtactttctg aacaaacaca agtctattgt gttttggaaa
    26161 ccggacgtac acaccagatc agagttcatt tagctcataa gggacatccg atcgttggag
    26221 accctctcta ccaaaataga acagctgaac gactgatgct ccatgcatta gaactgcaca
    26281 tgatccatcc atttactcaa gaaaaaatag tcggtaaagc tttacctgga ttatgggaat
    26341 agaaataaaa aagaacagag tcttatcagc agataatgat aagactctgt tctttttgtg
    26401 taatttgtct gcttactttt gatgttcgtg ttctgctaac agcaaactac cgataatccc
    26461 actttcattc ggcagtcccc aacgaacgat atattcactt acttgaggtg tttccatata
    26521 accacccatc aattcaacaa attgctgacg gattttttga aggagatgat cctgattcat
    26581 taccccaccg cctaaaataa tcttttcagg tgctaacgtc aacgtatagt tgatcagtgc
    26641 ttgagcaata taataagcct gaatagccca aacaggatga tcttgcggca gatcttgccc
    26701 tttgatccct gtgcgtgcct caatagacgg tcctgctgct aatccttcta aacaatcttt
    26761 atgataagga caagtacctt cataatggtc atctggatgg cgtttcaccc aaatatgtcc
    26821 catttcagga tgtgcgatcc ctgaaaaaat ttcttcattg atcacgacac ctgcaccgat
    26881 tcctgttccc acggtaaggt aaatacaact attttttcct tgtgctgctc cttgcttcaa
    26941 ttccccatat gcagcagcat tgacatccgt tgtccaagca gcaggaatat tgtagcgttc
    27001 cttgattgtg ccaagaaaat cgaattgtgc ccaccctttt ttgggagttg ccaaaacgta
    27061 tccgtaatta tcttttgttt tgtcgatacc aatcgggccg aatgagccaa tcccgattcc
    27121 cttcaattca taacgatcaa aaaattcaaa tacctgatcc aatgtttctt ctggtgttgt
    27181 ggtagggata cttaattttt ctaaaatttc tccaccatct gaaaccgcac acacaaattt
    27241 tgttccgcct gcttctattc cgccatacat tcgttttcct cctcgaatcc gattgtccga
    27301 atctcataag gttggattat tgggacaacc gcttgcttcg ttggttcttc aagcagattc
    27361 aatatgacag tttttccatt gtctttagtt atttccaatt tttctaaatg actagacata
    27421 ttgaaacctc ttactactac tttatcacta aacttgctgc gtttcaaagc tgtaattgca
    27481 aacgtttcac tttttatcgt taaatattgt tgttttgaaa tcaatgttcc ttcatgatgt
    27541 ttgatttgtt gagtggaaaa aggaatctgt gctgcgtatg catgcaaata agtagagaat
    27601 ttttcttctg gtccatgtag ttcaatgctg taattgaacc gatgctcacc tagacactgt
    27661 gcttctggtg ttggaaaata tccccaatcg cctagttctc ccacactgcg aagtagcgtc
    27721 actgcgattt gtccgtcttc tgttacttca tattcattca aaccaaagtt tccgactgtt
    27781 acaccgtact cttcatcatg aatattgaca aatgcttgct gatgttgcgg attcgtgggg
    27841 ttttcccaac ttttagacac atggtttggg cgcttcacca cttcaaaaat actatctgct
    27901 tcgtgatgtt ccacatgtaa ctttgtagga aacaagacac gtaagcgatg atctttcatc
    27961 tgattgtcga gagtcgtttc aaagtcgatt tttttactat cttttctgat tgtcatgatt
    28021 gtttttattt gaaggattcg tttttctttg gatcgttctg cttttcgata acgaaattcc
    28081 acaaccatct gctgttcttt ttctaaacgc tcgtctgcag aaacagggat ttccatttcc
    28141 tgtagaatac ttatttttgc tatttccggt gtatctacta atacttttac ttcagagttt
    28201 tctttgttac tggataggat tggctgatca cagaaaggtt gtttaaagat atactcattt
    28261 cctatatcgc cgatatcttc aaaaatatgc agattattca actttcgttt gtttttgcga
    28321 tcttccattg taatggtacc atttttttca atcgtcaaat gtagagggcc gttttcgatg
    28381 attcgtccac tctgatgaat cattgtttcc ttttcttctg ctttcgcttc cccttctaca
    28441 agcgcgaatg tttcccaaga aaatgctggc atgttttcta atggtaaagt cactttgatc
    28501 attcttgcca tatatggcac acggaaacgg tctttcggca aatcataacc aaattggaca
    28561 gtttcttcag ataaaatagc tggtatagtg gccccacttt ttgtttctac atgatactta
    28621 cgtttaggca aattttctaa ctcttgatat agttgttcag gtattccttc agcgaatctt
    28681 tttctttcta attcgatggt tatttctgct tcaccagttt tatccattcc tgcagtattt
    28741 acaatgacaa atggaaagct gtcttttggg aatccggtcg tatcgattgc tgcggtcagc
    28801 tgttctaagg aatcttgtgc aagatacttt ccaacttcat ctgctttttc aaaacgaggt
    28861 atcatttccc gatggacaga atcgactgaa caaccgcaaa tactgtcgtg aggatgattt
    28921 tgcattaatg tcttccaagc ataatctaat tgatcatggg gatagtttcc actaacttca
    28981 taagccatcg tcgcaagtgg ttctgtaata ttttctagct gtcgctggac cttggtattc
    29041 cattgtttta agtaaacccg agcagatgct gtattggcta aagtatacca gccatccgtt
    29101 tcttgacttg tcaattctcc ttctactgag cctaagtctt ctggcacatc tttttgaact
    29161 gcttccagat agtcagtaaa attagaatga ataaactcgt aatctggaaa cagttcattt
    29221 gcgagatgga ttgcttttga tatatctttt tgtaccggtt gatgatcaac accattcatc
    29281 atcaataagt ggttcgtaga ggcatattgt tctgcatccg ctaatttttg cttccaaaag
    29341 gcaagagctg cttctttttc tactggaatc tcattcccgt tgctatacca attggcaaat
    29401 agcagaccaa agattgctgt ttgatcaggt cctttccacc acatctctga gtattgggaa
    29461 gaatagtttt ctgcttccaa aacttgatta tcaaagccaa ttggttttac accacggcca
    29521 aacgctgctg ctgaaatacc agcttgtttc attaattgag gcgtctgtcc catatttcca
    29581 aatgtatcgg gaaaatagcc taacatgaca ggggttcccc atttacggct ttcttccatg
    29641 ccaatcagca tatttctcac gttcgattcc gagctgataa gaaaatcatc ttgcaagata
    29701 taaaatggtc caattctcaa ttttccttca ttgattgctt gttggattgc ctgtcttttt
    29761 tctggcctta cttgaagata gtcatctaaa ataatcgttt gcccatccaa atgaaaactg
    29821 ttgaaggacg gatcaacttc aaatagctct agtagatcgt ctatcaattc aatcagtcgc
    29881 atgtgatgct gttcgtaagc catgtaccat tcacgatccc agtggctatg agaaatgata
    29941 tatactttct ttttcattta cgatacctcc tacttttcta ctctaatatc aaagtaatcc
    30001 atgactaatt cgcaaaacat catatttgcc caagaaaacc attctctcgt ataattttca
    30061 ggattgttca catcgaatcc ttcgtgcata aggtgtgttc ctgcgtcagt tgctacaagt
    30121 aaattcaata tccgctcttt ttcagatttg tctgacgttg tcattccttc catagctaat
    30181 gcaatcggcc aaacgtattt ttctggcgta tgggaggatc caattccttt tgcgtacttt
    30241 ccttcataga aatatggatt ttctttactc aacaatgttc ttcttgtttg cagataatct
    30301 tcctcttgtt cagaacaata tcccaagtaa ggtgcggcga tcagattggg cacattactg
    30361 tcatccatga ctgtggcgtt tcccattcca tctacttcat atgcataaat cgtttgccct
    30421 ttttgattaa ccgttcgtcc gtgattctga atcccctctt ggatctcttt cttcaatttc
    30481 tgtgtttgct gtacaatttc tttatcatcg agtatcgttt gaaaaatttc ttccacataa
    30541 cctaacacga ccaccgcaaa catattggat ggtaccagat atccataacg gcaagtatca
    30601 tcgctaggac ggaaaccaga ccatgtcatt cctgtaaaag ctgtttttgc acctcgcccg
    30661 tcattgatca gcgtatcttc ttttcttgtc gtgtctcgta caaatgtata aggagagtgt
    30721 tcatgatctt gctctgtttt gaacacgtcc aagatttttt tgataccttc aataaaagat
    30781 tgattgaatt gatcagtttt tcctgtattt ttgtaaagaa gataggccaa ctgtattggg
    30841 tagcataaag aatcaatttc atattttctt tcccagatcc aatcgttcat ttttgtatga
    30901 tctgtttggt ggccagcacc gttagcttct tcgttgaagg cattcgcata tggatcgata
    30961 ttgatataaa aaaattgctt tttgactaac ccgctgatca tgtcagctaa ttcctcatct
    31021 tcttttgcaa tcactaaata aggacgaact tgtgcagtag agtctcttaa ccacatcgct
    31081 ggaatgtctc ccgtcaataa aaaggtggtt ccgtcatttt gctttttgac tgttgtcaac
    31141 agagtattgg caaatgctgc tgtaaaattc tttgcccagt ccgcatggat atccccacat
    31201 ttttgtgtga tatcttccat aaatttctgc accgattttg gtatatcttt ataagccact
    31261 aataaaacct tctttctatt tgatatatct ttaaagatta tacttcatac agccaattat
    31321 gtctacgctt tcaaaaaata tctttttatt tactttttca tccattatgg tattatttga
    31381 atatgatata tcattaagag gtgtttatat gaatcaacca ttgtaccaga caatatttaa
    31441 tgatttgcaa acggctattt taactggtga attacctgta gacagccaac taccaactga
    31501 aaaagaactt tcacgtacct ataaagtaag cagaatcact tccaaacgtg cattgactga
    31561 attagagcaa ttaggactta tttatcgcgt tcgcgggaaa ggcagtttcg ttaaatccgc
    31621 ttctccggaa cagattcttg cttctcctac aaaaacacga cggattctgt ttcttcttcc
    31681 atatgtcgct gatttgtctg ttggtgattt tagcaaggga ttgaaccctg tgatgcaaga
    31741 taaacagatt gatgtcatga tgacgacgct tgactttcta caaaataaaa cagcaaaaga
    31801 gctaatcaat gaatttgacg gattgattta ttatgctctt gatacagaac aacatcttga
    31861 cttacttttt gagctttcat tgaaagaatt ccctgtcgtc atattagata aaaaaatcta
    31921 tgagttacct tttcctaccg ttctttccga taatttccaa ggcggtgcgc ttgctgcctc
    31981 ttatttgatc gaacagggac attcaaaaat cgcttatttg tttggtacgc agactcatcc
    32041 acaatccgtt cgacagcgtt accttggtta ccttgaagca ttggacaaag ctggtttgac
    32101 tttccacact tcactggatg acaaaagcgc gacattagat gaattgcttt cttatatcac
    32161 taagtatgaa gtaactgctt ttgtttgtga aaacgatgtt gtcgctatcc agaccatgaa
    32221 tctcttgaga cagcatggtt atcatatccc tgaagatttt tcaattatcg gatttgatga
    32281 tatccaagca gcagctcttg ttgacccgcc actgacaacc atcgcacaag attttgaaca
    32341 gttaggtaag cttgcgggag atgctttggt cacttggtta gagacaaaag atcgaccatc
    32401 cgatagtaaa tttccagttt cattcatcaa aagacaatca acaaaggaga aataaaatga
    32461 atatcggtca aatagatact agacatgcaa cagcgaatca gccaaatttc tctaatggga
    32521 actgccagcc ttatactggc gttccttttg ggatgaatta ttttgcaccg caaactacgg
    32581 atcaaaacgg cagctggtgg tttcatcctg atgaccgcgt ttttcaaggc taccgtctta
    32641 cccatcaacc aagtccttgg atgggtgatt tcagtcatat gctgtttacg ccgatcaatg
    32701 gaaaactaca ggagaacacc cttttccatg ctcaaagttc ttaccgccca gaagaaagta
    32761 tattttgccc gactcatctg tcgatctgtc aattacgtta tggcatctgt tctacattga
    32821 tacctagcat gtatggcgga atcttatcta ttgattacag caaaaatgac agcggtttac
    32881 ttttatcctt tccgggacgc catcagctct ttgttataga tccttacaca gtggaaggaa
    32941 aaatcacaca ttttgctggt tcagaagatc ctgatttttc tttttatttt atattgagat
    33001 ttgaatatcc tttaacaatt gatgagttag ctgtttccaa caaaaatagc gactcgatcc
    33061 cgttgtattt tgaacagaca aaaaaacaaa ctatccgatt tgggacatct tttatcagct
    33121 cggaacaagc agcttataac ctggaactag aacaaaacca aagcaaagag acttaccttg
    33181 ctcacagtca agaacaatgg cagtcctatt ttgatcgtat acagctcaca caccatgatc
    33241 aacaacaatt gcggacgttt tatcataatt tatatcgggt gtttttattt ccacaaacat
    33301 tttatgaaat tgatcctagt gggcaaaaaa tccactatga tacgactagt cgatctataa
    33361 aacctggtgt tctctataca aataatgggt tttgggatac gtataaaacg gtttatcctc
    33421 tttattcttt gattgctgta gaaaaatacg aagaaatgct ggaaggtttt ttgaacagct
    33481 atagagaaag tggctatttg ccaaaatggc tgtctcctga tgaacgtgga cttatgcctg
    33541 ggacattgat cgatgcggtc attgcagatg ctgctgtaaa agatatccga cgagacttga
    33601 tgcctgaatt tctaacggca atggaaaaag gtgctacggt tcaaagcaaa catccaaatt
    33661 atggacgcca aggaacagaa gattacttga catatggcta tgttcctaat cactaccacg
    33721 aatcagtcaa tcacacatta gactatgctt atagcgattt ttgtattagc caagttgcca
    33781 aggtactaga agaagaggaa atgacgaaga aatatcagca gcaatcattg aactatcaaa
    33841 atatctttga ctatgagtca ggatttatgc gttcaaaaga taaagatggc aagttcagag
    33901 aaccattttc agctaatcgt tggggacaag attatgcaga aggaagtgct tggcaaagca
    33961 gctttgctgt ttatcacgat ttttcaggac tgatcggaaa atatggtggc agtgagagat
    34021 ataaagaaaa actcattgag ctgtgcaatc agcccccaac gtttgaccca ggaggatatg
    34081 gttttgagat ccacgaaatg agtgagatgg ccgcgataga atttggacaa ttagctattt
    34141 caaaccagcc aagttttcac tatccttact tgttcagtta tataggacaa cctgaaatgg
    34201 cacagccact gcttaaacag ttgatgactc agaccttcga cgattctcca atgggttacc
    34261 ctggagatga ggacaatgga agtatggccg cttggtatat cttcaatagt ctgggatttt
    34321 atcctgtcac gcctggaacc ggtgaatatg ttatcggtat gccattaatt gacaaggctg
    34381 ttttgctttt gtcaaatgga aaggaattga cgattgaagc aacgcctaat cggccgcagc
    34441 aacaatttat ccataaaatc aaacggatgg acaaagaata tacgaaatta tattttaccc
    34501 atcaagattt gctgaaaggc ggcacgatct cttaccaatt agggatcatc cctcacccta
    34561 agaaatatca agcaaaagat ttgccttttt ccttatcaaa ctataattaa caagaggttg
    34621 tgaaaaagct gtcctgcatc aagtaataag aacagccaag caaaaataga tcctcatatt
    34681 tttcgtttgg ttggtcttat taccgcagat gcaagctttt gaacaccgtt tattgagagg
    34741 ttgtgaaatc agcgttctga cctgagtatt agagagacaa atggcaaaat agttcctcat
    34801 attttgccat tttttgagct aataccgagg gtcagctgat tgaacaccgt ttagtggaga
    34861 ggtcgtgaaa aagctgttta gtttcgagta ttaagaaaaa ttttgtgaaa atggctttcc
    34921 acatttttgc ataaatttgg cttaataccg agaaactggc ttttgaactc cgtttatcga
    34981 gaggtcatga aaaagctgcc ttgcctcaag taataagaac agcccaaaat tagatttcta
    35041 gacaattttt tataagttat ttcattacat tggctttttc ttttatgatt ctccactgtt
    35101 ttctcggtac tctttagggg atctgccgac gattttcttg aataatttag agaaatacgt
    35161 ggtgttgttg tagccaatct cacttgctat ctcattgatg ttttgattac tgtgcaaaag
    35221 cagttgtttc gctttttcca tacgtaaatg gttcaaataa gctgaaaaac tttttttcgt
    35281 ttcttttttg aacagctgtc ctagatacat gacatttaaa tgcaggtttt ctgacacttc
    35341 tttgagtgta agtggttctt gataatgctg gtgcaggatt acgattactt tttccacatt
    35401 gtcactgtac tttttctgtc tctgatgctt tttgatgaat gacagaagta gctgctgaag
    35461 ttcttgcacc gtttttgcct gatggatttt ttcgattcct tgtaaatatt cctcatcttc
    35521 cagatcaatc aattcccgtt gaatctccat aaacagtaaa aaactggaat atcgtatatc
    35581 ttccggcatc atggctgcta actgaaactg ttcaaaaaaa tcagtgatca ttttttgagc
    35641 gacttccaat tgtttatttt gcagcacgcg tctgaaactt gaaaaatcga tggtttgatc
    35701 ttttgtttct gcttgtactg cgtaaaaaac atgctcgtct ttatctccat aaaattgatg
    35761 caactgtaga ttgtctttcg cttgttggaa tgtttcaggg attttgtcca aagaatctgt
    35821 ctcttccccg atactgatca gccattcctg atcaggaaag ttttcttgaa caaaacaaca
    35881 gaagccatca acttcatctg cctccaacaa caacgtgatc aatacacggt ctccgtaatt
    35941 tcgttgatag taaaagattt gttgccgttc ttgcaaccat ctattgataa gagtatcatg
    36001 agttcttgcc agttgaatca atactatcct atgctgcttc tcacttattt gtcctattat
    36061 ttcttcttca cttatttcat ccaattcttc attcaaccat tgactgaata acagttcttg
    36121 atagatttct tgttgatttt tttgttcatt ttgctgatct aaccgtgttt tgattttctt
    36181 gaggctttca accaattcga acttattcac gggtttcatc aaataattca cggcgcctaa
    36241 ttgcatacct cctttcaaat aatcgaattc ttggtatcct gataaaatca taaattcaaa
    36301 attatgacgt tctttttgag ccgcttcaat aaattccagt ccatttattt ctggcattgt
    36361 cacatccgtt actactaaat caatctgtcg ctgttcaagt acagctagtc cttctttggc
    36421 attctctgct gttgctacta tttgaaagcc taaactttgc caatcaatta tcttttttaa
    36481 cccattcaaa atcatgtatt catcatctac caataacact tgatacacgc tcatccctcc
    36541 ttgaatgtaa tagaaacggt cagtccacct gtctcattat tctcaagcga catataataa
    36601 ctgctgccga agctggcacg taaacgttca ttcacgtttt gcagaccgat agaaccgtga
    36661 agttccactt taggatgaga cagtttttct tcaatctgtt ttagccgttt ttcagtcatc
    36721 ccttttccat tatctttgat gatgatcctt acccgtttgc cttcctgcaa gactttgaca
    36781 cttagcgcat tatcgaatcg agtaaaatca ataccgtgtt taaaataatt ttcaactaat
    36841 ggctggatca caaatttagg tacttcgatc ttttccaaat caggatcgat cataaaatga
    36901 taggctactc gattaggata acgcatttga tacaaataga catatttttc gcaaaagtcc
    36961 agttcttctt ttaaagtgat cgttttctct tgattcgtat tgtttcgaag caaagcggaa
    37021 aacgcataca caacatctgc taattcttca cttccttcgc tgattgcata cattcggata
    37081 tattctaatg tattatataa aaagtgtgga ttgatctgtg cttgaagcgc ccgcatatgt
    37141 gcgtcttgct gtttgatttc taatttgtag atgtcttcta caaactgatt gatgctatcc
    37201 aacatttcgt tgataccgat tgaaagttcc ttcagttcat attccgtgtt tgttatgtcg
    37261 atccgcgtct ctaaattccc tgctgctgtt tgatccatgg cctgcatgac gatagatact
    37321 tgctgtgaat atcgtttgaa tgttttataa agaaagataa gcagtatatt gtctaaaata
    37381 atgataccaa tgatcagcgg ggcaagattt tttcccgttt cgatttttac acttttttta
    37441 tctagtacag ctaatatacg ataatcatca gacagttctt ggtgttgaac aaaattgttt
    37501 ttttccagat cgtcaatcgg caattgtgaa tttttcttca gactctctgt gatgatcatt
    37561 tcttgtcttt caaacgtttc tttagctatt ttattatcat ggaatgtgta tagtcgattt
    37621 gtcgttccag aaatcatgta taaagagagt ccattgaatg acgttagatt ttctaaaatc
    37681 atatctagtt ctttcttttg aaaaccaata aaaatacttc ctgactcagc tcctccttgt
    37741 gtgataggca acttgatata aaaggcgtct agtaattttg gtgtcccctt ttttatttcc
    37801 ccacctttgt tttctctaga agattcataa tactccggta gttgattcaa cacgatatat
    37861 atcgcactta gttcttcata atttgcgtag agatctttga tttgatttgg gaaagaaact
    37921 aattcatcgg tactgttttg ctgttcgtat acatacatca gatactgatc tatgggtttt
    37981 gttaagtagg tcgtcacatt ttcgatcttt tcggaattcc ctacaagctg gtttttgatg
    38041 atcctactca aggttgtttt atcattcagc attctgcttg tgctttgaac ggcatcactt
    38101 gtcatgtttt ctgcctgttt ttgattttgt tcccaagtat ggatactaaa aataacggta
    38161 gcagtcatcg ttacaccgat catgataacc gcgtatttcc gcaacagacg atttagtaag
    38221 gttccttttg aaaatagaat ccgaaactta ttcataactt tccaactgtt tatctaattc
    38281 ttcacgccaa tgtgttgtca tcgtaccatt ccaaaccgtc aaaagaccaa tgacaccaaa
    38341 caggatcaat cctttgaatt gccaagtcac gcctaaaatt ataccgctac caataattat
    38401 ttttagaaaa gacgaaaagc tggaaaaaac cgaaataaaa cttagcttta gcagattagg
    38461 aacagagatc gtatattcac tatctaaaat catactgtat tgataagtag caaataggca
    38521 gagcaaggca aaaagtaaaa taaaatcaat agctaaaaaa atgagccctt tgatttgaac
    38581 agataaatat aaattgtaac tcaataaagc agtaccagat aaaaataaac taaatagcag
    38641 attgcctctt aaaaaatttc ttttatatat attccagcct ctttttattg tgatttcttt
    38701 atactcgaat ccgtacaaat aaaatagttc attgactgtt ttccatgccg cacctaatcc
    38761 aaagatgatc ccgccggcaa atgtatatat ccagaaatac aaggttagtt ttataatgac
    38821 ccagatccgg ataaataact cttccaaaac cttccctaac atatcttctc ctccaaacat
    38881 taagtttata ctttctcatt atacaaaaaa aggttgggca atcagcacct tgacctgatt
    38941 attagagaaa caaacggaaa aatagttcct catattttgt cgttttgtga gcgaataccg
    39001 agggttagct gattgaacac cgtttaccaa gagtttgtga aatagccgct tagtttcgag
    39061 tagtaagaaa aattttgtga atatggcttt ccacattttt acatatattt agcttaatac
    39121 cgagaaactg gcttttgaac accgtttatt cggaacaaga ggttgtgaca cgtcttttgt
    39181 cacaacccct ttgtctttat tcattattat tcttgtcgga actcatcata ttgtttttgc
    39241 atttctttca ataccttatc atagccagcc ttgtccaaag cgtctttcaa tttcggtaat
    39301 gtttcatctg gatcaacagt ccctgtattc aatccatcta aatattgatt catgacatta
    39361 gaaatattac ttaattctgt tttgacactg tcagtgttga agctgaatcc taaaatcggt
    39421 gaagtttctg catcagcaat cgattgatca cgtttggcaa tcatgtcatc cgtaatagac
    39481 tcttgtgtat atagaatctt gttgtttcct gtattccaag cagacatatg tgtatttggc
    39541 tgataaccat ctagcaattt gattttatca tctgaaccat cgactttttc ccatgcttct
    39601 ccctcgatcc cccaaacaag cccattcaat aattctggat cactgttaag cagccctaga
    39661 agttctactg atttttcttt attttttgaa gtatttgata cgacaaaatt tgccatttgt
    39721 gcttgagctg tagattttag tggaacagta attgcttttg agacaagttc ttgttttgct
    39781 gcatttgtca aaattgtatc gccataatca tacggtcctt gtgtttctcc acgcattagc
    39841 caagtatttc cttctagtgg ataatcctta ttgctcgttg cagcgtcact tggaatgagt
    39901 ccttgtttgt accagctatg cattgtacgt aacagtccga tatacgtttc attatcgtat
    39961 tggttgatga ttttcgaatc atctccatcc agatcaatag caaatggtaa cgtattccct
    40021 aaaggataat caaagttccc ttggactttg aagccttgtc caatggcaaa agctgccgtg
    40081 tttggttctt tttcatggaa ttccttcaag actttttctg catcagcgta agattggatt
    40141 ccatcaattg aaagatcgta tttatctagt aaagctttgt taaaagtaag tacttgttga
    40201 gcaaagacat ttccattgac tgggaaagca tataattttc cattgaccag atttccttta
    40261 atatatgctt catctaaatc attataagct ttttcagcgt acttaggcgc taattcagtc
    40321 aaatcagcaa aggctccttt ttgggcattg gaaacatagt tgttcgcaaa ggcgatatca
    40381 taattttccc cggaagaaat gatgacgttc attttctttt cgtaatcgcc ccagccaatg
    40441 taattgatat tgacttttac accgatctct ttttcgattc gtttgtttgc tacttccatc
    40501 aattgatcga aattatctgg tttatctccg atttgataca tctgcaaagt gtccgaattg
    40561 ctagcttctg cctggtcttt agaaccagaa cctccgcatg ctgctaatgt tcccaacaac
    40621 aacgctgaaa cacctgtaaa ggctagtttc ttccataact tcatttttct tcctcctaat
    40681 tttatgattt atatttttgt ttctttattc ttttactccg ccgattgtta gacctttgac
    40741 aaaatgcttc tggaaaaatg gatagctgat cgcaataggc aacgtcgaga tgactacgat
    40801 cgccattctg gcagattctc ctggaactga ggctaatccg cctgtcaatt ggcttcctgc
    40861 tcctgaattt tgtgtcagat attgaatatt actttggatt ttcatcaaaa gatactgtag
    40921 tggaactagc ttatcatctt ggatgtatag taaagcattg aaccagtcat tccagtaacc
    40981 caatgcggca aataaactga ttgtcgcaat acctggaaca gctaacggta aaacgatctg
    41041 aacaaatatt cgcagttcgc tagctccatc gatccgtgcc gattcaataa tcgaatcaga
    41101 gaccgaacgt ttaaagaatg tgcgcatcac aataatattg aagggactga cagccattgg
    41161 caagatcaat gcccaaatcg tatcttttaa ctgaagaaga tttgtcataa caagataatt
    41221 ggccaccatc cctggtgaga acaacatcgt aatcaaacaa aaaacggtaa aaaaacgacg
    41281 gaatggaaag tttgaccgtg aaatcgcata agcatataaa gaagtagccg tgctattgat
    41341 cagtgtacct aaaacagtaa caagtacagt gacgaaaagc gcttggaaaa ttttatcctg
    41401 catctggtcg aacaagtacg tataaccaaa tatactgaac ttttctggcc aaaaactgta
    41461 cccgtgagtc gacaatatgg actcatcggt aaaagagatg gcgataacaa atataaatgg
    41521 caaaatacag gaaatagcaa agagtgcaat cattatatta gagaaaaaat tagccgtctt
    41581 attgaaagag cggacttgga ctctctccac tttctttttt ttcatttttt tcctccttag
    41641 aataatgcag aatcgctatc aaaacgtcgg gcgatcgtat ttgtgatcat caacaagaca
    41701 aagccgacaa ctgattggta aagtcctgca gcagccgtca taccaaaatc tcctgtggat
    41761 gtcaggccat tgtagatata cgtatccaaa acttgagtca catcatataa tgcaccggaa
    41821 ttcctaggaa tgttataaaa caggccaaaa tcagctcgga aaatgtttcc tacggctaaa
    41881 atcgttaaaa tcgtcattag tggaataagt tgtggtatcg tcacatgttt gatctgctgc
    41941 catttgctag ctccatcaac catagcagct tcataataag tcggatcgat tcccataaca
    42001 gatgcaaagt aaataatgct gttataacca atccctttcc atgtaccaat gaacaccaag
    42061 ataaacggcc aatatttcgg ttcattatac caattgacag cttctcctcc atgtccagtg
    42121 atccactgat tgaatattcc tttgtcagga cttaaaaaag cgtaaacaaa gtaactgata
    42181 atgacccaag atagaaaata aggtagtaag gacatcgttt gataaacttt aacgagtcgt
    42241 ttatttctca actcgctcat aatgattgca aatacgactg agatcagcag attcaaaagg
    42301 ataaatgcta cattatataa aattgtattt tta
//
LOCUS       DACDPS010000014        41426 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.014, whole genome shotgun sequence.
ACCESSION   DACDPS010000014 DACDPS010000000
VERSION     DACDPS010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.014"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..97
                     /locus_tag="IWJ07_000836"
     CDS             <1..97
                     /locus_tag="IWJ07_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9332435.1"
                     /translation="SFYQREGFEIQYSGLDEATGEKDYVMAWQQK"
     gene            276..506
                     /locus_tag="IWJ07_000837"
     CDS             276..506
                     /locus_tag="IWJ07_000837"
                     /inference="COORDINATES: protein motif:HMM:NF025559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HAP9332436.1"
                     /translation="MQDDDSGFTYEISRCLYFDTCKEHGYAEFCKVFCNHDWYAYGVL
                     KHHSRFIRKSTIAEDGTVCHDTIEKVTEKHFL"
     gene            540..722
                     /locus_tag="IWJ07_000838"
     CDS             540..722
                     /locus_tag="IWJ07_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylan 1,4-beta-xylosidase"
                     /protein_id="HAP9332437.1"
                     /translation="MAKEERFEKIYSQGALTTTEIWVDKETGVNYLYHACGYSGGLTP
                     LLDRDGKPVISQAINR"
     gene            835..2346
                     /locus_tag="IWJ07_000839"
     CDS             835..2346
                     /locus_tag="IWJ07_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332438.1"
                     /translation="MRRSGWTKWLIVIPAMLGIVLVTYVRYTTDIWGFGAFICQLIAI
                     LELAYGLRIAMLAQNRKKSYRLTPEERHEYARYLYEKQYHRYPAVANQMLLVMARMSV
                     LLNNYERAAQELADIRIDKFNPAQLKLYYYLKVVTAMAAGDATGIQESQMCYAGIPDT
                     KCTYPSKAELAMWIEQRAAAQMAEALKKAVPDKKEHPIRISIITLVLAYSTFFYGLWY
                     GIKRDMGYEVRYRFAEISITVITISLAVLLIWGIVSLYRHNCQALSIQFGKNKVALII
                     VYGAAAIFALLIIGMSGLSVVFGMDGTETVTGCDSKYTYITVQEDYGGERRYRTNNPF
                     VMQSMDGLWWSSDTIDEEDNQEEDTTELPDLDTENSTDSEDIQSGNTIEDYETEGQHD
                     GLTIQNAMLAVYNYLQEQNTLQNMAFSYTANAKGEVYAVVSETQETKNGNTVNVRYCL
                     YDNGSKTDADGNSCEELVLEKVYPDGNYETELVDFYLVNADTMQVTDEQKNTW"
     gene            2669..2893
                     /locus_tag="IWJ07_000840"
     CDS             2669..2893
                     /locus_tag="IWJ07_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3791 domain-containing protein"
                     /protein_id="HAP9332439.1"
                     /translation="MSEKTEITFMQTRLIRLASEEWHLPVEQIIHLFKEADVLGYIEK
                     CYGIFHCEGDEAVLEDITEFLQRKGIEISA"
     gene            2886..3452
                     /locus_tag="IWJ07_000841"
     CDS             2886..3452
                     /locus_tag="IWJ07_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3990 domain-containing protein"
                     /protein_id="HAP9332440.1"
                     /translation="MLELKDGFVLYHGSYCEVKEPDLAKCAKRKDFGQGFYLTTSKEQ
                     AESFLRTSIAKAIATGTIEEGQKFGYISTFEFKLSENLETHIFENADVDWLHCIAAHR
                     KKKMFIEVEREMAKYDIIAGKIADDATNATLTAYLAGAFGTAGDKVADDFCIRQLLPN
                     KLKDQYCFKTEAAIECLKFVKGEKIWLK"
     gene            3440..3670
                     /locus_tag="IWJ07_000842"
     CDS             3440..3670
                     /locus_tag="IWJ07_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332441.1"
                     /translation="MAEVTIPKEKMNYTIDLLITMVTDEIAEETGKDRNEVLTDFLCS
                     KTGKALYDEKTKLWCTGPAYIAELYMEELKKS"
     gene            3871..4236
                     /locus_tag="IWJ07_000843"
     CDS             3871..4236
                     /locus_tag="IWJ07_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015526708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="HAP9332442.1"
                     /translation="MSRAKNPKYESMSLEELKANMEKSRQELEAAIHHKNILEQRKKL
                     VERRERSHRLIVKGAEFEKVFPLSRDLEQEDVQDMMNQLQNSSYNKSIVRQAHIAALH
                     KDQQQIAEAVKKTEKGDDS"
     gene            4236..5963
                     /locus_tag="IWJ07_000844"
     CDS             4236..5963
                     /locus_tag="IWJ07_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HAP9332443.1"
                     /translation="MALYHFSVRNVSRGKGQMVVASAAYISGQRIFDSYYNKIHDYTS
                     KSGVIFTEILTPEYVPERLTDRETLWNEVEHVERNNKAQLAYSFDIALQNELTLEENI
                     RLAKEFCQEQFVARGMIVDLAVHEGKAENEEEPDNPHFHVLVPIRPFTEEGIWGNKQR
                     REYILDEDGNRVKDAKGKDMFNAVSTTGWNDPELLKEWRRAWTEKVNGKFRECHMASR
                     IDHRSYKEQGIDLIPTIHEGYEVRAMEKKGIKTVIGELNRAIRQFNQMFISLKESIQW
                     MKTAYEEMKAELDRRQNPTLLESLQDYYDKKTQGRPPLPNFYAEMKQRGKNLSNLQEF
                     AKSISYLQTHQIETMDDLQERIEELNGVVSVSKKEISEKRKQLKELENLEKMAEVIKT
                     NQPLIDEYNHFYFQKRRERYYQQHKKEINYYRKCERELKQHLDKNGKVPTARWKREKE
                     ELQAGIEELKADNQPYQEELTFVKKVQSCADIARRDREMAEADTSGRSEEKREKQVKF
                     PTFYTVQAKEILEENGKAEQQPLNQSEQNPEKKTSLLRKLDEKKKECAERDAKQQAVK
                     KKRNYDMSL"
     gene            5993..7909
                     /locus_tag="IWJ07_000845"
     CDS             5993..7909
                     /locus_tag="IWJ07_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9332444.1"
                     /translation="MSKMQYTEEQIIRANQTDLVSFLSAQGEQLGKSGKEYRWKKHDS
                     VTVSGNRWYRHSQGRGGYPVDFVMEFYNATFPEAVKMLTGEEGEGRNSTCPAPSPDFR
                     LPEKEENNDRIIRYLTENRGIEKNMVEEWIGSGDIYEEKKHHNVVFVGRDADGIPRYA
                     HCRGTGETKYRGDVAESDKSYGFCHRGTDNQLFVFEAAIDLLSFIQLFPKDWKKRSYL
                     SLGGISSAALMAFLSERPQITSVFLCLDNDHAGNEASEKLAIEIPDGYSVIRLKPSRK
                     DWNEILCDKNADRKKSIIETVTMKVPEKEELVPMLCYEDIEQTSVEWLWFPYLPFGKL
                     TIIQGNPGEGKTYFAMMLTAACTNRKTFPNMEEIEPFNVIYQTAEDGMGDTIKPRLVE
                     AGADLSRVMVIDDTEEALTLSDDRIEKAIRQNQVRLLIIDPVQAFIGADVDMNRANEV
                     RPVFRKLGMIAEKTGCAIVLIGHLNKSSGTQSTYRGLGSIDIMAAVRSLLFIGKVKKD
                     PTTRVLIHEKSSLAPPGETMAFKLGDEEGFRWVGAYEISADDLLDGKEGKPTETKLQR
                     GTKLIYELLADGNAVTIRELDEKAKAQGISQRTMREARSRMKEELDYRMNEKQENTIR
                     LKKQGRMGDGRILE"
     gene            8594..8776
                     /locus_tag="IWJ07_000846"
     CDS             8594..8776
                     /locus_tag="IWJ07_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332445.1"
                     /translation="MAVKKKIINIRVYFDGDQDAKEVFAEVIAQKIRDTKTKDKLAIM
                     KDKQYNDYEFSNLKSA"
     gene            8803..10500
                     /locus_tag="IWJ07_000847"
     CDS             8803..10500
                     /locus_tag="IWJ07_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9332446.1"
                     /translation="MNNRYMSNNIERNATWRVGVYCRLSKDDELTGESASISNQRDIL
                     VNYCQSQGWQVVDVFQDDGYTGLNTDRPDLQRLLKACEKGLVNLVITKDQSRLGRNHV
                     QTGFLMEEFFPKHGVRYIALYDNVDTFAGDNEIAPFKNVLNEMYSRDISKKVHASYHL
                     QATKGKFTGVVPPLGYQKDPEEKGHLLIDDETAPIVRKIFQWAVDGHGINFISRELER
                     QQIPCPSWWHRKRGLRTYYTKWEKKDPENGKYVWDESYLKSLLMNPVYCGDMASQKRY
                     YRFKIGNQGDKAPEDWITVRDTHEAIIPQDVFDLVQAKMKGRKRQTEQGEYSMFAGLL
                     RCAECGGALTLKKTHTKDQHEVYTCSTYIHKGKAHCTQHRVDADDLYDAVLTRIQECA
                     KAVTGDGTELENRVKELCEEDTQGHRESLEKLVSKQKDRLETLDRLIAKLYDDLINDK
                     ITESVFDKMLEKTQKEQTDIKKELFQNESVLNTEEKLDAQSQQWIDDISEYADIKELD
                     ANLLNRLISKIVISEPQEKDGTENYCRTPETVTMEIHFNLKPIPELGTIERGSGSHK"
     gene            10525..10656
                     /locus_tag="IWJ07_000848"
     CDS             10525..10656
                     /locus_tag="IWJ07_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005927774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332447.1"
                     /translation="MLIFSRLISSKYFLIKDIKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            10748..11611
                     /locus_tag="IWJ07_000849"
     CDS             10748..11611
                     /locus_tag="IWJ07_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332448.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            11642..12184
                     /locus_tag="IWJ07_000850"
     CDS             11642..12184
                     /locus_tag="IWJ07_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HAP9332449.1"
                     /translation="MFISTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVE
                     RLASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTR
                     GNAEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFAR
                     QKTPGWDVWGNEVECDLILT"
     gene            12198..12620
                     /locus_tag="IWJ07_000851"
     CDS             12198..12620
                     /locus_tag="IWJ07_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HAP9332450.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            12550..14949
                     /locus_tag="IWJ07_000852"
     CDS             12550..14949
                     /locus_tag="IWJ07_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HAP9332451.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            14981..16972
                     /locus_tag="IWJ07_000853"
     CDS             14981..16972
                     /locus_tag="IWJ07_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006060479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HAP9332452.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            16995..17246
                     /locus_tag="IWJ07_000854"
     CDS             16995..17246
                     /locus_tag="IWJ07_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HAP9332453.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            17236..18465
                     /locus_tag="IWJ07_000855"
     CDS             17236..18465
                     /locus_tag="IWJ07_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HAP9332454.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            18462..20543
                     /gene="topB"
                     /locus_tag="IWJ07_000856"
     CDS             18462..20543
                     /gene="topB"
                     /locus_tag="IWJ07_000856"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9332455.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            20692..24612
                     /locus_tag="IWJ07_000857"
     CDS             20692..24612
                     /locus_tag="IWJ07_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HAP9332456.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKID
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLTERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            24613..25557
                     /locus_tag="IWJ07_000858"
     CDS             24613..25557
                     /locus_tag="IWJ07_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332457.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            25561..25764
                     /locus_tag="IWJ07_000859"
     CDS             25561..25764
                     /locus_tag="IWJ07_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332458.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(26064..26300)
                     /locus_tag="IWJ07_000860"
     CDS             complement(26064..26300)
                     /locus_tag="IWJ07_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9332459.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(26483..26854)
                     /locus_tag="IWJ07_000861"
     CDS             complement(26483..26854)
                     /locus_tag="IWJ07_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332460.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(26851..28179)
                     /locus_tag="IWJ07_000862"
     CDS             complement(26851..28179)
                     /locus_tag="IWJ07_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP9332461.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(28140..28469)
                     /locus_tag="IWJ07_000863"
     CDS             complement(28140..28469)
                     /locus_tag="IWJ07_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP9332462.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(28727..29098)
                     /locus_tag="IWJ07_000864"
     CDS             complement(28727..29098)
                     /locus_tag="IWJ07_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9332463.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            29243..29428
                     /locus_tag="IWJ07_000865"
     CDS             29243..29428
                     /locus_tag="IWJ07_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP9332464.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            29940..30158
                     /locus_tag="IWJ07_000866"
     CDS             29940..30158
                     /locus_tag="IWJ07_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332465.1"
                     /translation="MRQERPEGVHTGPACVKSHTARCIRKKEAAHFAEAWGVPNKQNE
                     NAAGGQVVDQNIRKGYSDPLLASIIGMF"
     gene            30371..31030
                     /locus_tag="IWJ07_000867"
     CDS             30371..31030
                     /locus_tag="IWJ07_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HAP9332466.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            31033..32376
                     /gene="vanS"
                     /locus_tag="IWJ07_000868"
     CDS             31033..32376
                     /gene="vanS"
                     /locus_tag="IWJ07_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP9332467.1"
                     /translation="MERKGIFIKVFSYTIIVLLLVVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQNKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLGNIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            32547..33353
                     /gene="vanY"
                     /locus_tag="IWJ07_000869"
     CDS             32547..33353
                     /gene="vanY"
                     /locus_tag="IWJ07_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-B/VanY-G family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAP9332468.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLRWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            33371..34198
                     /gene="vanW"
                     /locus_tag="IWJ07_000870"
     CDS             33371..34198
                     /gene="vanW"
                     /locus_tag="IWJ07_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HAP9332469.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATIPEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            34195..35166
                     /locus_tag="IWJ07_000871"
     CDS             34195..35166
                     /locus_tag="IWJ07_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HAP9332470.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTARGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            35159..36187
                     /gene="vanB"
                     /locus_tag="IWJ07_000872"
     CDS             35159..36187
                     /gene="vanB"
                     /locus_tag="IWJ07_000872"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HAP9332471.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKFG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQIIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTMPGFTSYSRYPRMVAAAGITLPALIDS
                     LITLALKR"
     gene            36193..36801
                     /locus_tag="IWJ07_000873"
     CDS             36193..36801
                     /locus_tag="IWJ07_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HAP9332472.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            37388..37819
                     /locus_tag="IWJ07_000874"
     CDS             37388..37819
                     /locus_tag="IWJ07_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9332473.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
     gene            37826..38059
                     /locus_tag="IWJ07_000875"
     CDS             37826..38059
                     /locus_tag="IWJ07_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9332474.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(37981..38223)
                     /locus_tag="IWJ07_000876"
     CDS             complement(37981..38223)
                     /locus_tag="IWJ07_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332475.1"
                     /translation="MAHQPSGSAQTECVRMPYDAPPLSCGHIYQGAIRPPGKHRNGER
                     VSRFMLPGPVRQFVTIAWMSLISRRRFMASSRHTCG"
     gene            38480..38683
                     /locus_tag="IWJ07_000877"
     CDS             38480..38683
                     /locus_tag="IWJ07_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAP9332476.1"
                     /translation="MMNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVI
                     MNGNRIQIKRKQFEKIIDTLDTI"
     gene            38767..39960
                     /locus_tag="IWJ07_000878"
     CDS             38767..39960
                     /locus_tag="IWJ07_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9332477.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            40138..40368
                     /locus_tag="IWJ07_000879"
                     /pseudo
     CDS             40138..40368
                     /locus_tag="IWJ07_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320930.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase"
     gene            40496..41179
                     /locus_tag="IWJ07_000880"
     CDS             40496..41179
                     /locus_tag="IWJ07_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAP9332478.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
BASE COUNT        11425 a         9589 c        11053 g         9359 t
ORIGIN      
        1 atccttttat caaagagaag gatttgagat tcagtatagc ggtttggatg aagctactgg
       61 agaaaaagac tatgtaatgg catggcagca gaaatagaaa ttcaagtttt agtgtttgga
      121 aaattatgac ttagtgtgca agttgggaga aaatatggta acggaaaaag agaaaaaagc
      181 atgtcgattt attgccgaat ggctataaga ttgtgagaca gtctctggta gatgatctgg
      241 agggcgaaaa aaaggtatgt tgggagacag aaattttgca ggatgatgat agtggattta
      301 cttatgaaat cagcaggtgt ctgtatttcg atacctgtaa ggaacatggc tatgcagagt
      361 tctgcaaagt attttgcaac catgactggt atgcttatgg agtattaaag catcattcga
      421 gatttatcag aaaatcgacg attgccgaag atggaactgt atgtcatgat acaatcgaga
      481 aagtaacaga gaaacatttt ttataaaacc gaaaaattga ttatgggagg aaattgaaaa
      541 tggcgaaaga agagagattt gaaaaaatat attctcaggg agcattaact actactgaga
      601 tatgggtgga taaggaaact ggtgtgaatt atttatatca tgcatgtggg tattcaggtg
      661 gactgactcc attgttggac agagatggga aaccagttat ttctcaggct ataaatagat
      721 gaccttagaa acagcaactg ttatggtaag taacagcatc gtcacaatat gtatatggtc
      781 gattggtgtg tgcggtaata tactatattc agaaaaacaa ttcaggagat ttttatgaga
      841 agatcaggtt ggacaaaatg gttgatcgtg attccggcaa tgctaggcat tgtgttggtg
      901 acctatgtgc ggtatacaac ggatatctgg ggatttggtg catttatctg ccagctgatt
      961 gcaattttgg aattggctta tggtctgcga attgcgatgc tggcacagaa tagaaagaag
     1021 tcctatcggt taacaccgga agaacgacat gagtatgcac ggtatttata tgagaagcaa
     1081 tatcaccgtt accctgctgt agcgaatcag atgcttttgg ttatggcacg gatgagtgta
     1141 ctccttaaca attatgaacg ggctgcacag gagctggcag atattcgtat agataaattc
     1201 aatccagcac agttgaagtt gtattattac ctgaaagtgg ttacagcaat ggcagcagga
     1261 gatgcgacag ggattcaaga gagccagatg tgttacgcag ggattccgga tactaaatgc
     1321 acatatccgt caaaagcaga gcttgctatg tggatagagc agagagcggc agcgcagatg
     1381 gcagaggcac tgaagaaggc agtgccggat aaaaaagagc atccgatacg gatcagtatc
     1441 attactttgg ttcttgcgta tagtaccttc ttctatggat tgtggtatgg tataaaacgt
     1501 gatatgggat atgaagtgag atatcgtttc gcagaaatat cgattactgt tattacaatc
     1561 agtctggcgg ttcttttgat ctgggggatc gtgagtttat acagacacaa ttgccaggca
     1621 ttatccatac aattcggtaa aaacaaagtg gcattgataa ttgtctatgg agctgctgcg
     1681 atatttgcac ttttgattat tggaatgagt ggtctgagtg tagtgttcgg aatggatgga
     1741 acagaaacgg tgactggttg tgacagcaaa tatacgtata taactgtgca ggaagattat
     1801 ggtggtgaac ggagatacag aaccaataat ccatttgtta tgcagagtat ggatggtctg
     1861 tggtggtctt cggatacaat agatgaagaa gataatcaag aagaggatac tacagaatta
     1921 ccggatttgg atacagagaa ttcaacagat tcagaggata ttcagagtgg aaataccata
     1981 gaagattacg agacagaagg gcagcatgat ggtctgacaa ttcaaaatgc aatgctggca
     2041 gtttataatt accttcagga gcagaataca cttcaaaata tggcgttcag ttacacggcg
     2101 aatgcaaagg gagaagtcta tgctgtcgtc agcgaaacgc aggaaacaaa gaacggtaat
     2161 acggtaaatg tgagatactg tctgtacgat aatggttcca agacagatgc agatggtaat
     2221 tcctgcgaag aactggtgtt agaaaaagta tatccggatg gcaattacga gaccgaattg
     2281 gtagattttt atctggtaaa tgcagatacg atgcaggtga cagacgaaca gaagaatacg
     2341 tggtaaaata aattctgggg aagtaggtgc attgtatgac aatgagagaa aaaataaaac
     2401 tattatcgta aatgtttcaa gcggactggg ttcttttgga agtatgcaaa aggactccaa
     2461 gacataagag gtgaactgcg ctgatccagg tgccacggca acagatttga atgtcgacgg
     2521 aggaaagcaa acagtacagg cgcaatcgta aaacttgcaa tgattgataa aaccggacta
     2581 acagtaactt ttattagtcg atttggaaat atgccttggt aatcaacaat tccggtttta
     2641 ctgactaaga aaatgaggag gattcataat gagtgagaaa acagagatta cttttatgca
     2701 aacaagacta atccggcttg cttcagaaga gtggcattta cctgttgaac agattatcca
     2761 cttgtttaaa gaagccgatg ttcttggata tatagaaaaa tgctatggga tttttcattg
     2821 tgaaggcgat gaagctgtgc ttgaagatat tactgaattt ttgcaaagaa aggggataga
     2881 gattagtgct tgagttaaaa gatggatttg ttctctatca tggaagttat tgtgaagtaa
     2941 aagagccaga tcttgccaaa tgtgcaaaga gaaaagattt tggacaggga ttttatttaa
     3001 ccacatcaaa ggaacaggca gaaagttttc tgagaacctc aatagccaaa gccatcgcaa
     3061 ccggaacaat tgaagagggg cagaagtttg gatatatttc aacttttgaa ttcaaacttt
     3121 cagaaaatct tgaaacgcat atttttgaaa atgcagatgt ggactggtta cattgtatcg
     3181 ccgctcaccg aaagaaaaaa atgtttattg aggttgaacg tgaaatggca aaatatgata
     3241 tcattgcagg aaagattgcg gatgatgcaa caaacgctac tcttaccgct tatcttgcgg
     3301 gtgcttttgg gactgcaggt gataaagtag cagacgattt ctgtatcaga cagttgctgc
     3361 cgaataaatt gaaagatcaa tactgcttta aaactgaggc agctattgag tgtctgaaat
     3421 ttgtgaaagg tgagaagata tggctgaagt aacaattcca aaggaaaaaa tgaattatac
     3481 aatcgacctt ctgatcacta tggtgacaga tgaaatcgca gaagagacag gcaaggacag
     3541 aaacgaggta ttgacagatt ttttgtgttc aaagacagga aaagcactgt atgatgagaa
     3601 gacaaagctg tggtgcactg gtccggcata tatagctgaa ctgtatatgg aagaattaaa
     3661 gaaatcctga tatgggatgt gagtttaaat ttatacgaca atggtgacaa agactttcat
     3721 tccaaggagc aacaattaaa tatttcaaaa acgctgaggt cagcatttcc taatcacagg
     3781 acaacgacct cagtttttta tacccaggaa tcggcattca tgaataagcc ggttcttttt
     3841 gtttacagaa aaaagaagga gtacaagaaa atgagcagag caaaaaatcc aaagtatgaa
     3901 tccatgtcat tggaggaact gaaggccaac atggaaaaga gcagacagga actggaggca
     3961 gcaattcacc acaaaaacat tctggagcag cggaagaagc tggtagaacg cagggaacgt
     4021 tcccaccggc tgattgtcaa aggggcagag tttgagaaag tatttccgct gtccagagat
     4081 ctggaacagg aagacgtcca ggacatgatg aatcaactgc agaacagcag ttacaacaag
     4141 agtattgtgc ggcaggctca tatagccgcc ttacacaaag atcaacagca gattgcagaa
     4201 gcagtaaaaa agacagagaa gggagatgac agttaatggc actgtatcat ttttcagtaa
     4261 ggaatgtatc cagagggaaa gggcagatgg tggtcgcttc cgcagcctat atttccgggc
     4321 agaggatttt tgacagttat tacaacaaga tacacgatta tacgagcaag tcaggtgtga
     4381 tatttacaga aatcctgaca ccggagtatg tgccagagcg tctgactgac agggaaacac
     4441 tctggaacga ggtggagcat gtggaacgga acaacaaggc acagcttgcc tattcgtttg
     4501 acattgcact gcagaatgaa ttgacacttg aggaaaatat cagactggca aaagagttct
     4561 gccaggagca gtttgtggca cgtggcatga tcgtggatct ggcagtccat gagggaaaag
     4621 cggaaaatga agaagaaccg gataatcccc atttccatgt gcttgtccca atccgtcctt
     4681 ttacagagga aggaatctgg ggcaataagc agagaaggga atatattctt gatgaggatg
     4741 gaaaccgggt gaaggatgcc aaaggaaaag atatgttcaa tgctgtttct actaccggat
     4801 ggaatgatcc ggagctttta aaagaatggc ggcgggcatg gacagagaaa gtaaatggga
     4861 aattcaggga gtgccacatg gcatccagaa tcgaccaccg ttcctacaaa gaacagggga
     4921 ttgacctgat cccgaccatc catgagggat atgaagtccg ggcaatggag aaaaagggaa
     4981 tcaaaacggt gatcggagaa ttgaaccggg caatccggca gtttaaccag atgtttattt
     5041 ctctgaaaga atccattcag tggatgaaga cagcttatga ggaaatgaaa gcagagctgg
     5101 atcgcaggca gaacccgaca ctgctggaaa gcctgcagga ttattatgat aaaaagacac
     5161 agggcagacc accacttccc aatttttatg cggagatgaa acagagaggt aagaatctct
     5221 ccaatttaca ggagtttgca aaatccatca gttatcttca gacacatcag atagaaacga
     5281 tggatgactt gcaggaacgg attgaggaac tgaacggtgt tgtttctgtc agtaaaaaag
     5341 aaatttctga aaagcggaaa cagttaaagg aacttgagaa tctggaaaag atggcagagg
     5401 taataaaaac aaatcagcca ttgattgatg agtataacca tttctatttt cagaaaagac
     5461 gggagagata ttatcagcag cataaaaaag aaatcaatta ttaccgcaaa tgtgaacggg
     5521 aactgaaaca acatctggat aaaaatggaa aagtgccaac tgccagatgg aaacgggaga
     5581 aagaagaact tcaggcgggg atcgaagagc tgaaagcaga taatcagccg taccaggagg
     5641 aactaacttt tgtgaaaaag gtacagagct gtgcggatat tgccagacgt gaccgggaaa
     5701 tggcagaagc agatacttcc gggcgttcag aagaaaaaag ggagaagcag gtgaagttcc
     5761 cgacatttta cacagttcag gcgaaagaga ttcttgaaga aaacgggaaa gcagagcaac
     5821 agccgttgaa ccagtcggaa cagaatccag aaaagaagac ctctcttctg agaaaactgg
     5881 atgaaaaaaa gaaagagtgt gcagagcgtg atgcaaaaca acaggcagtg aagaaaaagc
     5941 gaaactatga catgagttta taacagtctg tcagcaggac agacgggaaa ggatgagcaa
     6001 aatgcagtat acagaagaac aaattataag agcaaatcag acagatcttg tttcttttct
     6061 gagcgcacag ggagaacaac tagggaaaag cggaaaggaa taccgttgga aaaagcatga
     6121 cagtgtaacg gtatccggaa accggtggta caggcacagt caggggaggg gtggctatcc
     6181 ggtggatttt gtgatggaat tttacaatgc cacatttccg gaagctgtca agatgctaac
     6241 cggggaggaa ggcgaaggca ggaacagtac ctgccccgcc ccgtcccctg acttccgatt
     6301 accagagaaa gaggaaaata atgatagaat catcagatat ctgacagaaa acagagggat
     6361 tgaaaagaat atggtggagg aatggatcgg cagtggggat atttatgaag aaaagaagca
     6421 ccataatgtg gtgtttgttg gcagagatgc agacggtatt ccaagatatg cccattgcag
     6481 gggaactggt gaaacaaaat accggggaga tgtggctgaa tctgataaat cttacggatt
     6541 ctgtcacaga gggacggata atcagttgtt tgtgtttgaa gcagccattg atctgctttc
     6601 ttttatccaa ttatttccca aagactggaa aaagcggagc tacctgagtc tgggtggcat
     6661 ttccagtgcg gcactgatgg cttttctttc tgaacgccct cagattactt cggttttcct
     6721 gtgcctggat aatgaccatg cagggaatga agccagtgag aaattggcaa tagaaatccc
     6781 ggacggatac agtgtcatcc gtctgaaacc gtcccgaaag gactggaatg aaatcctctg
     6841 tgataaaaat gcagacagga agaaatcaat cattgaaaca gttacaatga aagtaccgga
     6901 gaaggaagaa ctggtgccga tgttgtgtta tgaagacatt gaacagacca gtgtagaatg
     6961 gctgtggttt ccctatctgc catttggaaa actgacgatt attcagggca atccaggaga
     7021 gggaaaaact tattttgcca tgatgctgac cgcagcctgc acgaaccgta agactttccc
     7081 gaacatggaa gagattgaac cttttaatgt gatctatcag actgcagaag acggtatggg
     7141 agatacgatt aagccgaggc tggtggaagc aggagcagac ctgagtcggg tgatggtcat
     7201 tgatgataca gaggaagcat tgacgttatc cgatgaccgt attgaaaaag caatccgtca
     7261 gaatcaggtc agacttttaa tcattgatcc ggtacaggct tttatcggtg ccgatgtgga
     7321 tatgaaccgg gcaaatgaag tccgtccggt attccggaaa ctgggaatga tcgctgaaaa
     7381 gaccggttgt gcgattgtgc tgatcggaca tctgaataaa tcttcaggga cacagagtac
     7441 ttaccgggga cttggttcta ttgatatcat ggcggcggtg agaagcctgc tttttatcgg
     7501 gaaagtaaag aaagacccga caacaagagt actgatccat gagaaaagtt ctcttgcacc
     7561 tccaggtgag acgatggcat ttaaacttgg agatgaagaa ggattccgct gggtgggagc
     7621 ttatgagatt tccgcagatg atcttctgga cggaaaggaa ggaaagccga cagaaacaaa
     7681 actgcaacgg ggaacgaaac tgatctatga actgcttgca gacggaaatg cagtcacaat
     7741 ccgggaactg gatgaaaagg caaaagcaca gggaatctcc cagagaacca tgcgagaagc
     7801 cagaagccgg atgaaagaag aactggatta tcggatgaat gaaaagcagg aaaataccat
     7861 tcgattaaaa aaacaaggga gaatgggtga cggcagaatt ttggagtgaa cggcaggctt
     7921 tatagatacc ctatctgccg tctgccgtct gccggtgcaa ggcaatcaaa cggaaatagc
     7981 aggtatccat acagaataag gattgctgtg aatctggaac aaaaatgaat tgtaatatgg
     8041 cggatataat ggtatgatta tccggtaaca gcaatgttac ggggaagcac ctgtgacagg
     8101 gtgtggctgc ctcctgatat ttacagaccg gagcctttcg gggttctgag atgaaagaaa
     8161 ggaggtgcgt gatatgacag cttatgagat tatctcaata ttcttaggcg tactcagttt
     8221 actgatcgct tttggtagcc tgttaattgc gattattgcc tttcttgatc ggaagaacaa
     8281 gaggaaataa aaatgcctat cctgtctgcc aacaggatag gcggtggtcg taagaccatt
     8341 tagtcaactc aggcggcatc cactttggag tgggtgcttt tcctataaca aatgtaccac
     8401 acattcctct tgttttcaat gggaaaatta aaatatgcag ttcatctgac agggatttat
     8461 ccctgtcttt attatatccc caatttctga aatgttaata caaaaatttt aatatatgta
     8521 accaacagtc tgggcagtta atctgtaaaa atggattagc tgctcttttt ttgcaaaaaa
     8581 tggaggtttt acaatggcag tgaagaagaa aattatcaac atcagagtat attttgacgg
     8641 tgatcaggac gcaaaagaag tgtttgcaga ggtgatcgca cagaaaatac gtgacacgaa
     8701 aacaaaagat aaacttgcaa taatgaaaga taagcagtat aatgattatg agttcagtaa
     8761 tctgaaatct gcgtaggatg tgcaaagtaa cggaggtatc agatgaacaa cagatatatg
     8821 agtaacaata tagaaagaaa tgcaacctgg agagttggtg tatactgccg actttccaaa
     8881 gatgacgaat tgaccggaga gagtgccagc atctccaacc agagagacat actggtgaat
     8941 tactgtcagt cacagggctg gcaggtggtg gatgtgtttc aggacgatgg ttatacagga
     9001 ttgaacacag accgacctga tttgcagaga cttctcaaag cctgtgaaaa aggactggtg
     9061 aatcttgtaa ttaccaaaga ccagagccga ctgggacgaa accatgtaca gacaggattt
     9121 ctgatggagg agtttttccc aaagcatgga gtaaggtata tcgcattata tgataatgtg
     9181 gatacctttg ccggagacaa tgagattgcc ccatttaaga acgtgctgaa cgagatgtat
     9241 tccagagata tttccaagaa agtccatgcg tcctatcacc tgcaggcaac caagggaaag
     9301 tttacaggtg ttgttccacc actgggatat cagaaagacc cggaggaaaa gggacatctc
     9361 ctgattgacg acgagacagc accgattgtc cggaaaattt tccagtgggc ggtggacggt
     9421 catgggatta actttatatc cagagaactg gaacgccagc agattccctg cccgtcatgg
     9481 tggcaccgga aacggggact ccgcacctat tacacaaaat gggaaaagaa agacccggaa
     9541 aatgggaaat atgtatggga tgaatcctat ctgaaatccc tgctgatgaa tcccgtttac
     9601 tgtggtgaca tggcttccca gaaaaggtac taccgtttca agattggcaa tcagggagat
     9661 aaagctccgg aggactggat cacagtcagg gatacccacg aagcaatcat tccgcaggat
     9721 gtatttgatc tggtacaggc aaagatgaaa ggcaggaaac gccagacaga acagggagaa
     9781 tacagcatgt ttgccggatt actccgctgt gcagagtgtg gcggtgcgtt gacgttgaag
     9841 aagacgcaca ccaaagacca gcatgaagtt tatacctgtt ccacttatat ccataagggg
     9901 aaagcacatt gtacccagca ccgggtggac gctgatgacc tgtatgatgc agtccttacc
     9961 agaatccagg aatgtgcaaa agcagttacc ggagacggaa cagaactgga aaaccgggta
    10021 aaggagttat gcgaggaaga tacacaggga cacagggaaa gtctggagaa gctggtatca
    10081 aagcagaaag accgcctgga aacgttagac cggctgattg caaaactgta tgatgacctt
    10141 atcaatgaca agattacaga atctgttttt gataagatgc tggagaagac acagaaggag
    10201 cagacagata tcaaaaagga acttttccag aatgagagtg tgctgaatac ggaagaaaaa
    10261 ctggatgcac agtcccagca gtggattgac gatattagcg agtatgcgga catcaaagaa
    10321 ctggatgcca atcttctgaa ccggctgatc agtaaaatcg ttatctcaga accgcaggag
    10381 aaagacggaa cagaaaatta ctgccgtact cctgaaacgg tcacaatgga aattcatttc
    10441 aacttaaaac caataccgga gcttggaacc atcgaaagag gttctggctc ccataaataa
    10501 gaaaaatcta tcaaaattta atctatttta atcttttcaa ggctcatttc gagcaaatat
    10561 tttctcataa aagacataaa acagcttgtc gctggcggcg gcgtggccct gatcggtgtc
    10621 actctggttc ccctgctgtc cgggctgctg ggctgatcct ggcctttgtc cctgtccaaa
    10681 cccttaacta atcgcctccg cgtcctcccg gccagaccgg gagggcgcgg gaaaggaggt
    10741 gctacccgtg attagtgaaa tcattgaaga atggataaaa ggcatcctca tggacggtat
    10801 cacgggcaac ctgtccgggc tgttcgatac ggtaaatgcc aaagtcggag aaatcgccgc
    10861 cgacgtggga cgaaccccgc aggcatggaa cagcggtatt ttcaatatgc tgcacagcct
    10921 gtcggaaacc gtgatcgtcc ccatagccgg ggccatactc gcccttgtca tgtgctatga
    10981 gcttatccag atgataacgg aaaagaacaa tatgcacgac tttgacagct ccatgttctt
    11041 ccgctggata ttcaaatccg cgtttgcaat cctgatcgta acgaacacat ggaacatcgt
    11101 catgggcgta ttcgacgcca cccaaagcgt cgtcaatcaa agcgccggtg tgattatcgg
    11161 gcagacatcc attgatttta acaccctgat accagacctt gaaagccaat tggaggctat
    11221 gagcgtgggc gctcttttgg ggctgtggtt ccaaaccctt gttgtgggcc tcaccatgaa
    11281 tatcctttcc atctgcattt tccttgtgac ctatggccgc atggtggaaa tctatgttgt
    11341 cacgtcgctg gggcctatcc cccttgccac aatgggcaac gcggaatggc gcggcatggg
    11401 ccaagggtat ctgaaatccc tgctggcgct gggcttccaa gcctttttaa tcatggttgt
    11461 ggtgggtatc tacgcggtgc tgatacagaa tatcggttcc gcttccgaca tatccggcgc
    11521 aatctggggc tgtatgggct atacggtgct gctgtgcttc tgcctgttca agaccggcag
    11581 tatatcaaag gccgtattcg gggcgcattg atcccaaaat ccaagcatac acaggaggaa
    11641 cttgtttatt tcaacgtaca acatcattta cgctgacccg ccgtggcggt atgactgcaa
    11701 aaacgtacag ggcgcagcgg aaaatcacta ttccaccatg agcattgacg agctgtgcgc
    11761 cctgccggtg gaacggctgg cgtccaaaga ctgcctgctg tttttgtggg ccacattccc
    11821 catgctgccg gaagctctgc ggttaatcaa agcgtggggc ttttcattca agaccgtggc
    11881 ttttgtatgg ctgaaacaga accgtaaaag ccttacatgg ttttacgggc tgggccgctg
    11941 gacaagggga aacgcggaaa tctgcctgct ggcaacacac ggcaagccga agcgtcgttc
    12001 tgcctctgtc catcagttta ttatcagccc gattgaacaa cacagcaaaa agccggatgt
    12061 gacccgtgaa aaaatcgtag agctggcggg cgatttgccc cgcgtggagc tgtttgccag
    12121 acagaaaacc cccggctggg acgtgtgggg gaacgaggtg gaatgtgacc ttatcctgac
    12181 gtgaaaggag gtgccaaatg gcttatgtaa ccgtccccaa ggacttaacc catgtgaaaa
    12241 gcaaggttgt gtttgggctt accaaacggc aactgatctg tttttccggg gcgctggtga
    12301 tcggcgtccc cttatatttt ctcacccgtg actatcttac caacagcgcg gcggccctgc
    12361 tcatggtatt tgccatgctg ccgggctttt tgcttgccat gtacgagcgt cacggccagc
    12421 cccttgaagt ggtgattatg cagatcatcc agtgctgctt catccagcca aaggagcgtc
    12481 cctatcagac cgacaacacc tacaccgccc ttgtgcggca ataccagatg gaacaggagg
    12541 taaaggccat tgtccaaaaa ggaaatccca agagaaaccg aaaaaacaaa gctgacccgc
    12601 gcccagcgaa aagaaattga cgcggtaatc cgtaaatata agggcgacgg caggccccac
    12661 acggcgcagc agagcatccc ctacgaggtc atgtacccgg acggcgtgtg ccgggtatcg
    12721 cccggcgtct tttccaagtg cattgagttt gcggacatca gctatcagct tgcccagccg
    12781 gacacgcaga cggctatctt tgaaaaactg tgcgacctgt ataactatgt ggacgcctcc
    12841 attcatatcc agttttcttt cctcaatcgg aaagttgacc cggtgcagta cgccaagagc
    12901 tttgagatcg cgccgcaggg ggacgacttc gacgacatcc gggccgagta caccggcatt
    12961 ctgcaaaagc agcttgcaaa cggcaacaac ggcatggtaa agaccaaata cctgacattc
    13021 accatcgagg ccgaaagcgt gaaagcggcg cgagccagat tgaagcgcat cggctttgac
    13081 ctgttaggct atttcaagag catgggggcc gtagcccatg tgatggacgg ctgggaacgg
    13141 ctgaacctgc tgcatggcgt gtaccacccg gacggcgaga tattcaattt tgactggaag
    13201 tggctggccc cgtccggcct ctccaccaag gattttattg ccccgtcctc cctgtgcttc
    13261 ggcaatgcaa agacattcgg catgggcggc aaatacggcg cggtaagttt cctgcaaatc
    13321 ctgtccccgg agctgtcgga cgatatgctg gccgacttcc tcaatacgga aagcggtgtt
    13381 ttagtcaatc tccatgtgca ggccattgaa cagaccaagg cgatcaagac catcaagcgc
    13441 aaaattaccg accttgacgc tatgaagatt gcggagcaga aaaaggccgt ccgcagcggt
    13501 tacgatatgg acattcttcc cagcgacctt gccacctacg gcgaggacgc caagaagctg
    13561 ctgacaaagt tgcagacccg caatgaacgg ctgttccagt tgacgttcct tgtcctcaac
    13621 gtggccgaca ccaagcaaaa gctgaataac gacgtgttcc aagccgcagg cgtggcccaa
    13681 aagcataact gtccccttgt ccggctggac taccagcagg aacagggcct tgcaagcagc
    13741 ctgccgctgg gcgtcaatca gatcaagata cagcgcagcc ttacgacttc cagcgtggcc
    13801 gtgttcgtcc cctttgtgac gcaggagctt tttcagggcg gcgcggccat gtattacggc
    13861 atcaacgcca agtcccgcaa catgattatg ctggacagga agcaggcccg ttgccccaat
    13921 gccttgaagc tcggcacccc cggcagcggc aaatccatga gctgtaaatc cgagatcgtc
    13981 agtgtgttct taacgacgcc tgacgatatt tttatttcag acccggaggc cgaatattac
    14041 ccccttgtca aacggctcca cggacaggta atccggctgt cgcctaccag caaggacttt
    14101 gtgaacccgc tggacatcaa cctgaattat tcggaggacg ataacccgct ggccctgaaa
    14161 tccgactttg tgctgtcgtt ctgtgagctt gtcatgggcg gcaagaacgg gctggaagcc
    14221 attgaaaaga ccgtgataga ccgggctgtg cgcgtgatct accggcccta tctggcagac
    14281 ccccgcccgg agaatatgcc gatcctgtcc gacctccaca aagcgttgtt agatcagcat
    14341 gtcccggagg ctgaccgggt ggcgcaggcc cttgacctgt atgtgtccgg ctccctgaac
    14401 gtatttaacc accgtaccaa cgtggacatc ggcaaccggc ttgtgtcctt tgacattaag
    14461 gagctgggca agcagctcaa aaagctgggg atgctcattg tccaagacca gatatggggg
    14521 cgcgtcacgg ccaaccgaag ccaaggaaag gccacatggt attttgccga cgagttccat
    14581 ttgcttttga aagaggaaca gacggcggcc tattctgcgg agatttggaa gcggtttagg
    14641 aaatggggcg gcattccaac aggtgccacc cagaacgtca aagacctgct ttcctccccg
    14701 gaaattgaaa atatccttga aaacagcgac ttcattacgc tgctcaatca ggcgtccggg
    14761 gatagaaaaa tccttgcgga acgcttgaat ttgtccaccg aacagcagaa gtacatcgac
    14821 aattccgaac ccggcgaggg ccttttgatc tttgaaaacg tggtgctgcc ctttaccaac
    14881 cccatcccgc acaacaccca gctttacaag attatgacga cccgcttaaa cgaggtggcg
    14941 ggagtatgac ccggcgacgc cacaaaggag gtgaaccacc atgaaagagc tgaaagccaa
    15001 ggcgaaagtg acgcaggcca tgacccgcga cgggctgacg ctggaaaatc aggccaccgg
    15061 cgaggtggag aacatttcca gccgggaggc cgaacaggat ttgtccgcgc cggggccggg
    15121 cggcaccgcc gataagctgc tggaacgggc cgacgccatc catgacggcc ataaggccaa
    15181 gaaagccgcg aagcaggccg gggctgtggc cgccgagggg gacgctgccc tgcaccggcc
    15241 atccgcccgg ctccaactgt cggaggaaga acgggccgac ccggatttac agccctatat
    15301 ccgaaaatcg gacaaacggg ccgataagct ggataaggcc cgcgccgcca tccccaagaa
    15361 gcgcgtcccg gtcaaggaaa ccgtctttga cgaggcaagc ggcaaagcaa agaccgtcct
    15421 gcactttgac aaacaggaca aggggcctcc ctccctgaaa cccaatccgg caggccgtcc
    15481 tgtttccgag gtttttctat tcggccacgg gaaaatccat gaagtcgagc atgaaaacgt
    15541 gggtgtggag ggcggccata agggggagga acttgccgag cggcaggccg gaaaagccat
    15601 ccgcagcacc atccggcacc acaggctgaa accctactgg gacgcggaaa aggccgagcg
    15661 caggctattg tctgccaacg cggagtattt ctaccagaaa tctttgcggg ataacccgga
    15721 gatcgcacag gcggccagca accccatttc ccggttctgg cagaaacagc agatcaagcg
    15781 ccagtacgcc aaggccgccc ggacagcggg ccagaccgca ggcgcggcgg cacagggcgc
    15841 ggccaagaca gccggggcct ccacgaaagc ggccaaggcc gcagccaaag aaagtgaacg
    15901 ggcggcctcc tttgtggccc gccactggaa aggggcgctg gccgtccttg tgttggggct
    15961 tttgatgctg cttatcatcg gcggcttaca gtcctgcacc gccatgttcg gcagcgccgg
    16021 aagcggcatc acggcgtcct cttacttttc cgaggatagc gatatgttgg ccgccgagga
    16081 cgcctacgcg gagctggaag ccgagctgca agactatctg gacaactacg aggccaccca
    16141 tgactatgac gaataccatt ttgaccttga cgagatcagc catgacccct atgtgctgat
    16201 ttccattctg tcggcgctcc atgacgggat atttaccatc ggggaggtac agggtgattt
    16261 gtccatgctc tttgacaagc aatatatcct gacagaaaac gtcgtggtag aaacccgtta
    16321 ccgcaccgaa accgatacat ggacggacgc ggagggcaac acccatacag acacctacca
    16381 agtgccgtat gactattaca tctgctacgt cacgctggaa aacttcgacc tgtcccatgt
    16441 gccagtctat atcatggacg aggaaacgct aagtttctat tccctgtata tgtccacgct
    16501 aggaaaccgg cctgatctgt tttccggcag ccagtacccc aacgcctcca ccctgacgcc
    16561 gccgacctac tacgacgtac ccccggaggc tttggaggat gccacctttg ccgccatgct
    16621 caaagaggcg gaaaaatata tcggttatcc ctatgtgtgg ggcggcagct ctcccagcac
    16681 cagctttgat tgcagcggct tcatttcgtg ggtaatcaat catagtggat ggaacgtggg
    16741 acggctgggg gcgcagggcc tatataacat ctgcacccct gtttccagtt cgcaggccaa
    16801 gcccggcgac cttgtatttt tcaagggaac ctacgacacc cccggcgtgt cccacgtcgg
    16861 cctgtatgtg ggcaacaatg tgatgctgca ttgcgggaac ccgatttcct acacgaacct
    16921 tacttcaaac tactggcaat cccattttta cagttacggg cgtttacctt aacgccagaa
    16981 aggagctttc aattatggca accaaacttg accgcatcga aaaggacatc cagaaaacca
    17041 agtctaagat cgcggagttt cagaaacagc ttcgggagct ggaaacgcag aaaaccgaac
    17101 aggagaattt gcagatcatc cagcttgtgc gcggcatgaa catgaagccg gaggaatttg
    17161 ccgcgttctt acgcagcggc gcaatgcagg ccgcccctgc cgcaacccct taccatgaac
    17221 aggaggacaa tgcccatgaa gaataagacc agacggattt cccgtattct ccccgcactt
    17281 ctggccgtga tgctctgcgt cacggccttt cccgttaccg cccttgcagg cggcaatgac
    17341 ccggcaccgg ccccgctgcc ggaggctaca accgaacccg ctacgggcgg tttggagcct
    17401 gaaacagacg aaaccggcac cgccacggac gggacagaaa ccgaggccga aaaagatacc
    17461 ggccctctga ccggcaagga catcaccgac ctgttttccg cgctgttcgg ttccaaggtg
    17521 agcattgccg ccacggacga cggcatccag atcaccaccg gcaccgacaa aaaagaacct
    17581 gaacagaccg gcaccgtcac caccaacggc ggcaggctga atgtccgcac cggcgcgggg
    17641 cttgacaaga ccgcgttcac ccagcttccc aatgggacga cggttgaggt agtcggcaca
    17701 gacggcgact ggattaaaat tctcttgccg gagcgcatcg gctatgtcca ttccgactat
    17761 atgaccgtca gcgaaaagga agtagcggct accggcgagg gcggtttttc cctctccatt
    17821 gacccggagg aaattgcttc tctgctggaa ctgttcaatg gcggcggggc cagcgccgcc
    17881 ctgtccccgg acgggaacct gtcactgatt gacgacatcg gcagcgccgc gaaaaatggc
    17941 aagcagttta tcaccgtgga aaccaaaaac ggcaatgtgt tttatctgat tattgaccgg
    18001 gacgacgagg gaaaagaaac tgtccatttc ttaaatcagg tggatgaagc cgatctgctg
    18061 acgctgatgg aggacgggga aaccgtcacc cccgctgctg tctgctcctg taccacgaaa
    18121 tgcaaggccg gggctgtgaa cacgaattgc ccggtatgta aaaccaacct gaccgagtgc
    18181 agcggcccgg agccgcagga accccagccg gaggaacccg aagccccgga ggaagaaccc
    18241 aagggcggcg cgggcggcct gatcgtgttc ctgctggtgg cgctggcggg cggcggtgcg
    18301 gccctttact atttcaagtt caaaaagccc aaggctgaaa ccaccggcag cgacgatttg
    18361 ggcgagtatg attttgggga ggacgaggat ttggaggacg aggaacccga aaccgagttt
    18421 gacccggaag ctgattttgt gagtgaacag gaggacgagg aatgacatat aaacttgtaa
    18481 tcgcggaaaa acccagcgtg gcccgcagca ttgcgggcgt gatcggggcc acggagaaac
    18541 acgacggcta tttggagggg aacggctatc tggtgagctg gtgcatcgga catctggtgt
    18601 cctttgccga cgccggacgc tatgacgagc gtttcaaaaa atggcgctat gaggatttgc
    18661 ccattctccc ggaaacatgg cagtacatcg tccccgacga caagaaacag cagtttgacg
    18721 ttctctgttc gctgatgcag cgccccgacg tgaccggcct tgtgtgcgcc accgacgccg
    18781 ggcgcgaggg ggaattgatt ttccgtttcg tctaccagat ggcgggatgc caaaagccct
    18841 ttgagcgcct ttggatttcc tctatggagg acagtgctat taaggacggc tttgcccacc
    18901 tgaaaccggg cggcgactat gatccgctgt atcaatcggc cctgtgccgg gccaaggccg
    18961 actggctgat cggcattaac gccacccggc ttttttccgt cctgtaccac aagaacctga
    19021 ctgtgggccg cgtccagacg ccgaccttaa aaatgctggt ggacagggac gccaagatta
    19081 ccgatttcaa gaaagagaaa tatcacatcg tccatatcac ggcgggcggc gcggacgcgg
    19141 taagcagccg cttttccgac gctgccgagg caaacaccgt caaggcggct tgtgcaaaag
    19201 cgcaggccgt ttgcgtttcc gtgacgcggg agaaaaagac ggaacagccg cccaagctct
    19261 atgaccttac caccttgcag cgggaggcta accggctttt cggctatacc gccaagcaga
    19321 cccttgacta tgcccaggca ctgtatctaa agaagctgct gacctatccg agaacggata
    19381 gtcaatatct taccgacgat atgctgcccg ccgtggaaag ccttgtgtcc ggcctgtggg
    19441 agctggtgcc gtttgccaag ggcctgaacc tttccccgca gtttgaccgc atcctgaaca
    19501 gcaaaaaggt gtccgaccac cacgccatta tccccaccgc tgaatttgtg aagcagggct
    19561 ttggcactct ggccgagagt gaaaaaaagc tgctgtccct gatctgctgc aagctcttat
    19621 gtgccgtggc cgggccgcat atctatgagg ccgtcaccgc cacatttacc tgtgccgggc
    19681 aggagtttac cgccaagggc aagaccgtcc tttcccccgg ctggaaagag attgaacgcc
    19741 ggttccgttc ttccttaaag accgacgccg acgaggacgc cgaggcggtg cgggagctgc
    19801 cggagctttg cgagggccag acctttgcag acgtggccgc ctctgtcacc gagcatttca
    19861 ccacgccgcc caagccctac accgaagata cgcttttgtc cgctatggaa cgggccgggg
    19921 ccgaggatat gccggaggat gccgagcgca agggattagg cacaccggcc acccgcgccg
    19981 ccattctgga aaagctggtg cagatgggct ttgtccagag aaaaggcaag cagctcattc
    20041 ccaccaagga cggcatcaat ctggctgtgg tgctgccgga ggcgcttact tctccccagc
    20101 ttactgccga atgggaaaac cggctgaccg agatcgccaa ggggcaggcc gatcctgacg
    20161 aatttatgga cggcatcgag acgcaggcgc gggagctggt gcagacctat tcctgtatca
    20221 gtgaggaaaa gcagaaattg tttcaggccg agcgtgttcc tatcgggacg tgtccccgct
    20281 gtggtgaggc tgtctatgag ggcaagaaaa actactactg cggaaaccgg gcctgccagt
    20341 tcgtcatgtg gaagaatgac cgattttttg aggaacgcaa gaaagcgttc accccgaaga
    20401 tcgccgctgc gctgctcaaa accggcaaag tcaaggtaaa gggcctctat tccgtaaaga
    20461 ccggcaaaac ctacgacggg acggtgcttt tggctgatac cgggggcaag tacgtcaact
    20521 accgcattga acacaggagc taaaggacag taacacagca agcataggga gtgtggctac
    20581 actcccggta aaatcccccg gccgcgtccc tgccgggccg gggatttttg cttgttgggg
    20641 aagtccccaa ccccctattt tttgaaacag aacagagaaa ggagtgaccg cgtgaacgaa
    20701 gtattttcta tccaagtcca taaccggcag tttttggaac agggcaaaca aggcgtctgg
    20761 ctggatttgc ccaccacaac cgaaacgctg caagccgccc tgcgggatgt tggcattact
    20821 gccgacaatc cgcaggattt ttttatcaac gggtattcct gcccggagga cagacacctt
    20881 gccattccct atgacatggt gctggccgcc gacgtggacg agctgaattt ccttgccgcc
    20941 cggctgggcc agcttgacgc cgccgagatc gcagagctga acgccgccct gcaaaatccc
    21001 cgtggcggct ttgagaatat cggccagatc atcgactaca ccgaaaatgt ggattactat
    21061 gtgcatcttc ccgacgttag gggggatgga cagttgggcg actactacct gaaccgttcc
    21121 ggcatggtgg atatgccgga ggaatggaaa gccgggattg atacggcgca gtttggacgc
    21181 cacattgccc agcaggaaca aggcgttttt acccagtacg gctatctggt gagaagcggc
    21241 gacgaatggc agcgcgtcca tgagggccag ccggtgccgg aggaataccg ggtgatgcac
    21301 tacccccagc cggaagctga ccgggaggaa ccgcaggccc agcagccgga ggcatccgaa
    21361 agccccgaca aagcgccgcc cccggttatc ccgatcatgc tggacggcat gaacagcaaa
    21421 gaacgcatga aagtgattac tgaccgtctg gaaaccggcg tacaggagct ttttgaaagt
    21481 agccgctata tggcttactt atccgctatg tccaagatgc acgattacag tctgaacaat
    21541 acgctgctga tcgccatgca gggcggttcg attgtggcgg gctataacag gtggcaggat
    21601 gaatttgacc gccatgtgaa gcggggcgag aaaggcatta agatactggc cccggccccg
    21661 ttcaaggtta agcagcagat ggagaaaatc gacccggcca cccagcagcc cgtgatcggc
    21721 gcggacggca agcccgtgac cgaagaacag gaagtgacgg ttccggcctt taaggtggtg
    21781 acagtctttg acatcagcca gaccgagggc aaggattttc ccatgcccta tatcagtgaa
    21841 cttaccggcg acgtgcagca gtatcaggat ttctttgccg cgctggaacg gacatcaccc
    21901 tatcccataa gcattgagcc gatttcagac ggggccaagg gccgctgctt ctacgcaaaa
    21961 cagcgcattg ccatcaacga gggcatgagc gaattgcaga ccctaaagac cgccatccat
    22021 gagattgccc atgccaagct ccacgccatt gaccgggagg cccccgccga ggaacaggcg
    22081 gcccggccag accgcagcac ccgcgaggta caggccgaaa gcgtggccta taccgtctgc
    22141 cagtattacg ggctggacac gtcggactat tcttttggct atgtggccga ttggagcagc
    22201 ggcagggagt tatccgagct gaaagcctcc cttgaaacca tccgggccac ggccagcgaa
    22261 ttgataaccg agatcgacgg ccattttgcc cagcttcagc aggagcgcga ggcccggcag
    22321 gaacaggagg ccgtcacggt tgacgttacc ccggaggcag cggccccggc gcaggccgag
    22381 gaagaacagg ccccggccct tgatccggca gccgagccgc ttgtgactgt gctatggagc
    22441 gaaagccccc agctaaagga cggccagcaa atgccgttgc atgaggccga cgcgctgttt
    22501 gcccgtctgg atgcagcata tccggcagag gcaggctatg acaaaaccgc gttccgcatt
    22561 gactgtatct ttcagggcga gccggacagc tacgaggggc ggcaggattt tggagatagg
    22621 gacggttccc tgatcgaaca tatccagagc taccaccaat tttacgcgca ggacgaaaac
    22681 tggaaaaagc atgtaatcgc tacaaccggc atagagggct gggagcagga caaggcccat
    22741 cgggatatgc tgttaggcga gtttgtcccc tatatgaagc tgcattgcag ccttgcccaa
    22801 atggaacaga cggcgcaggg ccgcttacag tccggggaaa ccctgacgcc ggaggaaacg
    22861 gcctatttgg gggcgcttct ctcttatgtg caggagtgcc gccccatgct caatcagggc
    22921 cagtatcagt tgccggagcc gccgaaactg gctgacttcg accagagctt gcaggactac
    22981 aaagcacagg tacaggccga gatcgcgcag gaggccgccg cagccggtat gaccgtggag
    23041 gaatacgcgg cggctggcta tgaagcaccc caacaggaca ccttttccat ctaccagctt
    23101 cgaggcgggg aggaaacccg cgactatcgg tttgagccat acgagcgttt acaggccgca
    23161 gggctggccg tggacagtgc caactatgac cttgtttata cggcaccgct ggataaaagc
    23221 acgtctttgg aggatattta ccgcacattc aacatcgacc acccggcaga ttttagggga
    23281 cacagccttt ccgtttccga cgtggtggtg ctgcaccaga atggacagga caccgcccac
    23341 tatgtagaca gcttcggcta tatggaagtg ccggagtttt tgcgggaaaa tcccctgcgt
    23401 accgccgaac tgtctacgga gcagaacgaa aacatgattg acggcattat caacaatacg
    23461 ccgcccgaac cgcagccgga ggaacccgaa cagctatctt ttgaggatgt ggcagttgct
    23521 tccggccaga cacaggagga acccccggag gctgcgcccc cgcaaacggc tgtccgctat
    23581 tatcccatca atgagggagc cgcccgccgc gccaaggaag ccatgagctt ttctgactac
    23641 cggcccggca gcgccacggc ggaataccgg cactatgtcg atcaggccgt ggaaattgcg
    23701 gagcgccaga aaaagcgtgt ggagccagag tatcacgaaa agatagacag cctccttgac
    23761 acctacgccc gcaagctggc cgagaacatg aataagggct atgagattac cgcccgcgtc
    23821 ccctctgtcc tgattgccgg gccgtccaat ttcccggtac gggcgaaaga aaagcagaac
    23881 gccgccagtg accgcaacat ggaggaattt cagtacattc agggcttgct tgacaaaatc
    23941 cgcagcaccg gcatgggtgg tatcagcgcc gacgatccgc aggccgtttc caagctggaa
    24001 aagaaactgg aaaagctgga agcgtcgcag gagttgatga aggccgtcaa tgcctactac
    24061 cgcaagcatg ggacgctgga cggatgcccc catctgaccg aaagaggcat tgaaaacctg
    24121 aaagcggata tggcgtcggg ctggcactat gagaaaaaac cgttccagtc ctggcagctc
    24181 tccaacaata acgccgagat acgccggtta aagggccgca ttgaggaatt gacccgccaa
    24241 aaggaggcgg cctatgtggg atgggagttt gacggcggca ctgtggagat caaccgggtg
    24301 gccaaccgcc tgcaaatctt ctttgagggg aagccggatg ctgctgtacg cgacgagctg
    24361 aaaagcaacg gtttccgctg gtcgcccaag gccggggcat ggcagcggca gttaaacgac
    24421 accacaatcc gcgtcgctga ccgtatcaag tgcatccagc ccctttccgg cgaaaagccc
    24481 agcgccttac agcttgcggc ccgcagggag aaagaaaagc cctctatccg ggcgcagctc
    24541 aacgcggcca agaccgaaca ggccaagcag ccaccggcgc gggaaaaatc aaagggattg
    24601 gaggtagagt aaatgcagaa gtttgaaaat gtggacgttc tggccgcgct ggaacagatc
    24661 atgcggcaga acaccgcgtt ttatcaaagt gactttggca ttgacaaaga cattatcagg
    24721 cgggatgccg ccagcgatca ggccgcagac aagaccctgc tctggatgtc ccggccctcc
    24781 gggacgtact gtttccggga acgtgatgtt ttcctgaaag acacccgaca gtacaacaca
    24841 tggaaatttt acggggagca gacccgtgac aagatactgg cctacgcggt ggagctgacc
    24901 ggcacacagg gcggcactat ccggggcaat ctctatgagc tggactatcc gcagcatttc
    24961 cggcacgtca ttgaaaccgc ccagccggtg agtgtgaacc ggctctttta tgagcatggc
    25021 acccgtgaca tcccggagag ccagcgtttt gacggaagcc ctgaccgggt attgggaaac
    25081 tttttgtact atgaggccca gccccatgac ccggctgtgc tgcaggaggc gctacggacg
    25141 gagcagcaca gccgggggcg ggccgtcccc ggcgatttca aagtccacgt caccgctttg
    25201 catgacagcc ggattgaaac cgaggcccgg cgtattgtgg acagcctgaa agatctgtcc
    25261 gcgcctaaca gccccaataa aacgcatttc atggtggaga tctccccgca ctttgcggcg
    25321 attgcttcca ccaaggataa tgaccggctc tttgccatgc tgccctataa atccctgtgt
    25381 tttaccggga tgaaaggcag gcatggcgtg tatgccgtga ttaaccggga cgagcgccgg
    25441 gatgtgagta ttcgcaggcc ccggccctct atccgcaagc agctatccga cagcaaacag
    25501 gccgccagcc caaaaaaggc ggcggccaga accaaaaaga atgaattgga ggtataagga
    25561 atgatgcact ttaccgtgga ggaagaaaac ctgatctgta tgtaccacaa tgcagaccgg
    25621 cgcagaacga taaacaggct caccgccgtc atgccggtta tggatgggga tatgcgggcg
    25681 caggcccagc agaccctttc caagctggaa cgcatgacgg atgccgacta tgacggccag
    25741 aagttccact tcacagacga ataacgaaca gcagacgccg ggcgcggcgg ccatgtggtc
    25801 gcttgcgtcc ggcgtctttt ctttttgcct attcctacgc atttagaacc cggtattgtc
    25861 ggggatagga tgaagtattc gcttgcccct gcaaacatgc gggagaagcc ttgcagcggg
    25921 cggttttcaa tcccttaaat gcgtaggttg cagctataat tagacccctg aaacagatat
    25981 agccgcgcag cgggtgtatc tgtttcaggg gtgtgcaggt gatagccgca aagcggcgta
    26041 agagggtgca gccctcttgc cgtctatctc tgttccagcg ggaagctcgg caatgcttcc
    26101 agcagtttca gggaaatgcg ctgccgtata tcttcgtcaa tgactttctt ctttttgccg
    26161 tttgcctgtc tgatctccac cgtggaaagc tcgttgatgt agtcagcata atgggccaac
    26221 actttttccg tggcccacct ctcaccggca acggcggctt tgatcgtttc atagtccagc
    26281 agcttttctt gtccgtccat cctgataccc ccgtcagact gattttttct atgataccat
    26341 attcggaagc atggaaccag agaaaacggc gcttttctgc tggtaaattc tacgcattta
    26401 gaacagcact tcggcgggga taggataaaa cacccacata ccccgctgac catgccggaa
    26461 aagccttgaa atataagggc ttttacagct ctaagtgcgt aggtttccag tatcttttcc
    26521 gctggcgctc ggcctctttg cggcggcgca tccgtttgga gcaggccgcg cagtatttgg
    26581 cccggttaga cttcggcgtg aacgtggccc cgcactcaca gcagcgtttc acttcgtccc
    26641 ggtctttggt gatctcggca tacagggccg catccagcgg gaggacagcg gccttaaacc
    26701 agcggcacag aagcgaatag gaaatgctct gtacgcaaat acattcttcg ccgtcgtcca
    26761 gcaacaggca gttgccgtca caatagttgc agcactcatg cgtcagcttc cgggccttgc
    26821 gatattgagg gtaattcatt cgtggaatac tcatagctct tgcgtccatt cctttcccgg
    26881 cgtcatacgc aaaatgccgt ccacattctg cttgatgatg ccgtattcct gcatctgctt
    26941 tttcgcttcg ccgtacttct catacagccg ttccttttcc tcggccaatc gcttgtactc
    27001 ggctttcagg gcgtacagat cggggagctt tttcacaccc tgtcccttta gggcctttgc
    27061 cgcagcttca tacaaaatca acgtgctttc atgctcacgc cggaattttg ccttatccgc
    27121 tgcattgcgg tattccatag ccacgggcca gagctgcttg taggtagaaa tattctttat
    27181 cagcaccgcc atatccgcaa gccggtgttc catagctttg agggtagcgg cggcctcgtc
    27241 gctggcggcg gtgatctcgg ccactttgct ttccaaatcc gggtaggtgt ctatcccatg
    27301 ttcagtcagg aaattcaaag tctttgcggc ctgcttcaaa ttgtggagct tcgcccacct
    27361 ctcatagcct gccgactgct gggccttaat gttgttttcc agatcaatcc gcaaagaaat
    27421 ccccttgcgc tctttgggcg ctctggtgat aacgcggcct ttgatacgct ctttgatggc
    27481 ttcctcggtg taggcttccc ccaatgtctt gcagcgggta aagcgttcct gtccgggagc
    27541 gcggaaagaa ataaactttc ccggcttgat ctcatagccc tgcgcctgca tgagccgcag
    27601 gaaatcatca aaatcttttg cctgcgggat agcggcgtca atggctgctt tgagcttcgc
    27661 tttccagctc gtccctttcc gctgggcgtc ccactcggca tagctcttgc ccttactctg
    27721 gccgggcttc accacggaca atccgttttc cttgcacagc ctgtcgctgg tgcgccggat
    27781 ataggcatag ctctgtttgt tggaaatgta cttgcgctga tttaccatat ccacggcaca
    27841 gaaaataatg tgattgtgga cgtggccctt atcaatatgg gtagtcagca catagggata
    27901 tttcccttgc agcacttcct cggcaagctg cttcccgatc tcatgggcct gttccggcgt
    27961 ggcttcgccg ggggcaaagg attgtatcag gtggtggccc agattattgc ccttttggta
    28021 tgcctgcgcc agcagcattt caaattcaat gtccgctgtt tcataagagc aggcaaagga
    28081 ggccaccagc agcttgccgt cagttttgtc cgggttctcg atgtagtcaa gggctttttt
    28141 cagcgttgac ttgataggat gtatctttgt aactgccata tctgcgccag cgcccccttt
    28201 atatcctcga tgtcctgtgc gtacaccggc cccataccgt tcagcctctt tgcaatctga
    28261 ttgacgttga cgccgatctt ctgtatctcg gcggtctgcg cccggatgtc gctggtgtcc
    28321 atgttgacga tatagccgtc aatgagcatc ttccgggcgt aggctgaaaa gttcttcgta
    28381 tgcagcagcg ccattttctg ttcaatcata gcccgttcct ccggcgtcac agggacgcgc
    28441 agcacgattt ttcgctttct gtttgccatg cttcaccgtc ctttccattc aggggtttgg
    28501 ggatttcccc aacaagcaaa acggacgcgg cccggcaggg acggggcagg ccgtttttcc
    28561 gggagtggct acactcccta tgcttgctac gttactgttt ctccactaac aagacgtttc
    28621 caaagcccca aactacccgc aaaatagccc tctggaaaga aaaaagtgca aaaaaataga
    28681 agctacgctg tgcagctccc gctaaaagta aaacagagga tgccagttac acatcctccg
    28741 tttcattctt cttcggcttt tcaattagta tggcgctgtc aatcgcgccc tcgatgatcg
    28801 gcaggtattc ttcggggcag agtttcagct tgtggctgac cctttgccgc agttcgcttt
    28861 cctcccgcat gatctccggg ttaaagtagc gttccacagg aagcccgcag gctttaataa
    28921 gctgtatgac aaccggcaga ctgggaatgg ccccgtcgtt ttcgatgttg gcaagatagc
    28981 gccagtcaat atttattttc tctgccattg ctttgcgcga aacgcgcttt gcttgccggg
    29041 ccgctttcac atccgcgccg aaagtttcaa agccgggaca atcttcaata ttcgccatat
    29101 cgcacctccc acttacattg tatatttcat actctgcccg tggaatgttg gtatatgcac
    29161 ctttcttaaa gtttatattt cacattttgt ttcttttatt cttcgagcaa aagaattata
    29221 atgtaccctg tggaggtgta ctatggacta catgacgttg aaagaggcag gcgagaaatg
    29281 gggtgtgccc ccacgatgga taaactacta ttgtgctgcg ggacgcatcc ccggcgctgt
    29341 aaagatggcg acgatctggc tcattcccaa aactgcggaa aagccgattg acaggcgtac
    29401 aaagaaagga aaggcggtta ctgaatagta taatgaaacc ggcgcatttt ggatatggtt
    29461 ttgaagagag agaaagcatg gtattttgtc acggaaaaac tataagatga tagatggcag
    29521 gctgctccag accgataaaa agtattctca tctgaaacag aaagaaaaga tttacacatg
    29581 gatgtatgaa gaagcgaaac tttatcatga cagaaatgga aaatgcccga aacaaagacg
    29641 aggaagctgc tgctgtatca gccgtatatg gtagaattga aaaggccggc atctggattc
    29701 tttatggtga agtctccaga cattggataa ctgctggtct aatgcgcctt tgagtatggg
    29761 gtgattgatt ttaataccgg ggaggtacgg ctttttgagg aaaatattct gggatttaca
    29821 tttggcgatt attacatttc ctgtaactgc aaggacacgg atgatgaaga tatgaggaat
    29881 atagacttgc tgattttcta ctgtcttgaa cggtattagg actgaatctg ctgcgcggag
    29941 tgcggcaaga gcgaccggag ggagtacaca caggccctgc ctgtgtgaaa agccataccg
    30001 caaggtgcat tcgcaaaaaa gaggccgcgc attttgctga ggcttggggt gtccccaaca
    30061 agcaaaacga gaacgccgca ggcgggcaag tggtggatca gaatatccgt aaaggatatt
    30121 ctgatccatt gcttgccagt attatcggga tgttttgaca accgctgtaa tcgaaacatc
    30181 ccaaacatta agaaggtgtt aattattatt atcttgattt aattcgttta aaccaataat
    30241 atagatacat acaccatgaa aagaagcgag tatcccaatg acggatgatt aaaatccgaa
    30301 aagaaatcgc tttgaaacta cagggaaact acagactgtt atgttatctt cttaaaatgg
    30361 agggattttt atgtcgatac gaattctact tgtcgaggat gatgatcata tctgtaatac
    30421 agtaagggcg tttctggctg aggcaggata tcaggtggat gcctgcacag atggaaatga
    30481 ggcatacacc aagttttacg aaaacactta tcaactggtt attcttgata ttatgctgcc
    30541 cggtatgaac gggcatgaac ttttgcgtga atttcgtgcg aaaaatgata ctcccattct
    30601 gatgatgaca gccctgtcgg atgacgaaaa ccaaatccgg gcgtttgatg cagaggcaga
    30661 cgactatgta acaaagccat tcaagatgca gattttacta aagcgggtgg aagccctgct
    30721 gcggcgcagc ggtgcgctgg caaaggaaat ccgtgtcggc aggctgacac ttctgccgga
    30781 ggatttcacg gtactttgtg acggtacaga gctgcccctg acacgaaaag aatttgaaat
    30841 ccttttgctg ctggtgcaga acaaaggcag aaccttaacg catgaaatca ttttgtcacg
    30901 tatatgggga tatgactttg agggtgatgg cagcacagtc cacactcata tcaaaaattt
    30961 gcgggccaag ctgccggaaa atatcatcaa aaccattcgc ggtgtaggat accgattgga
    31021 ggaatcataa taatggaaag aaaagggatt ttcattaagg ttttttccta tacgatcatt
    31081 gtcctgctac tggttgtcgg tgtaacagca acactgtttg cacagcaatt tgtgtcttat
    31141 ttcagagtga tggaattaca gcaaacagta aaatcctatc agccattggt ggaactgatt
    31201 cagaatagcg ataggcttga tattcaagag gtggcagggc tgtttcacta caataaccaa
    31261 tcctttgagt tttatattga agataaagag ggaagcgtac tctatgccac accaaatgcc
    31321 aatacatcaa atagttttag acccgacttt ctttatgtgg tacatagaga tgataatatt
    31381 tcgattgttg ctcaaaacaa ggcaggtgtg ggattgcttt atcaagggct gacaattcgg
    31441 ggaattgtta tgattgcgat aatggttgta ttcagccttt tatgcgcgta tatctttgcg
    31501 cggcaaatga caacgccgat caaagcctta gcggacagtg cgaataaaat ggcaaacctg
    31561 aaagatgtac cgccgccgct ggagcgaaag gatgagcttg gcgcactggc tcatgatatg
    31621 cattccatgt atgtcaggct gaaagaaacc atcgcaaggc tggaggatga aatcgcaagg
    31681 gaacatgagt tggaggaaac acagcgatat ttctttgcgg cagcttctca tgagctaaaa
    31741 acgcccatcg cggctacaag cgttctgttg gagggaatgc ttggaaatat cggtgactac
    31801 aaagatcatt ctaagtatct gcgcgaatgc atcaaaatga tggataggca gggcaaaatc
    31861 atttccgaaa tactggagct tgtcagcctg aatgatggga gaatcgtacc catagctgaa
    31921 ccgttggaca tagggcgcac ggttgccgag ttgctgcccg attttcaaac cttggcagag
    31981 gcaaacaacc agcggttcgt cacagatatt ccagccgggc aaattgtcct gtccgatccg
    32041 aggctgctcc aaaaggcact atccaatgtc atattgaatg cagttcagaa cacgccgcag
    32101 ggaggcgagg tacggatatg gagtgagcct ggtgctgaaa aatgccgcct ttttgttttg
    32161 aacatgggcg ttcacattga tgatactgcg cttccaaggc tgttcacccc attctatcgc
    32221 attgatcagg cgcgaagcag aaaaagtggg cgaagcggtt taggacttgc catcgtacaa
    32281 aaaacgctgg atgccatgag cctccagtat gcgctggaaa acacctcgga tggcgttttg
    32341 ttctggctgg atttaccgct cacatcaaca ttgtaaatat ttaaaactta aatgattttg
    32401 accgacaggt ataaccctgc cggtcttttt ttgctgctac aggaaaacta cagactggct
    32461 acagggaaag tacagatgcg cttgccataa taacaatcgt accagccaca aatcgtagtt
    32521 ttattgcaaa ggaggcattc gatcaaatgg aaaaaagcaa ctatcattcc aatgcggatc
    32581 atcacaaacg gcatatgaaa caatccgtgg aaaagcgggc ttttctatgt gcgttcatta
    32641 tttcgttcac agtctgcacg ctgtttttga ggtggagact ggcttccgta ttggaggcaa
    32701 cacagatacc gcccatccct gcaactcata caggcagcag cactgacgta gtggagaatt
    32761 tggaggaaaa cgctcttgcc accgccaaag aacagggaga tgaacaggaa tggagcctga
    32821 ttttagtgaa caggcagaac cccatccccg cacagtacga tgtggagctt gagcaactat
    32881 caaatggtga gcggatagat attcggattt ctccctatct tcaagatttg tttgatgccg
    32941 caagaactga tggagtttac ccgattgtcg catccggata ccgaacaaca gaaaaacagc
    33001 aagaaattat ggatgaaaaa attgccgaat ataaggcgaa aggctacacc tctgcacagg
    33061 ctaaagcgga agcagaaact tgggtggccg tgccgggaac gagcgagcat cagcttggtc
    33121 ttgctgtgga tatcaatgcg gacggaattc attcaacagg caacgaggtt tatagatggc
    33181 tggatgaaaa cagctatcgt tttggtttta ttcgtcgcta cccaccagac aagacagaga
    33241 taaccggtgt gagcaacgag ccgtggcatt accgatatgt tggcatcgaa gctgccacag
    33301 agatgtacaa ccaaggggtt tgccttgagg aatatttaaa accagaaaaa tgagaaaagg
    33361 atgttatgtt atggacagaa aaagattgac acagcgtttc ccgttcctgc ttccaatgag
    33421 acgggcgcag agaaaaatgt gcttttatgc gggaatgaga tttgacggct gtcgctatgc
    33481 gcagacgata ggggaaaaat cgctttccca tttgctcttt gaaacggatt gcgcattata
    33541 caaccacaat accggatttg acatgatata ccaggaaaac aaggtgttca acttaaagct
    33601 ggcagcaaag accttaaacg gcttattgat aagaccgggg gaaacctttt ctttctggtg
    33661 gctggtacgc catgcggaca aagatacccc ctataaagac ggccttacgg tgaccaatgg
    33721 taaactcacc accatgtcgg gcggcggtat gtgccagatg agcaatttac tattttggat
    33781 gttcctgcat acgccattga caattatcca gcgcagaggt cacgaagtaa aggagtttcc
    33841 agagccaaac agtgacgaga ttaaaggggt ggatgcaacc attccagagg gctggattga
    33901 tttaaaagtg cgaaacgata ccgactgcac ctaccaaata tgggtgacct tagatgatga
    33961 gaaaatcatc ggtcaggtgt ccgccgacaa agagccccaa gcattataca aaattacaaa
    34021 tggcagtatc cagtatgtcc gtgaaagtgg cgggatttat gaatatgccc aggttaaacg
    34081 gatgcaagtt gccttaggta ccggggaaat aatagattgc aagctgcttt atacaaacaa
    34141 atgcaaaatc tgttatcccc tcccggaaag tgtggatatt caggaggaga accaatgaga
    34201 aaaagtgtgg gcattactgt ttttggatgc gaacaggatg aggcaaatgc tttccgcgct
    34261 ttatcgccgg attttcatat tatccctacg ctgattagcg atgcgatatc ggcagacaac
    34321 gcaaaattgg ccgctggcaa tcaatgcgtt agcgtaggcc ataagtccga ggtttccgag
    34381 gcgacaattc ttgcgctgag aaaggtcggg gtaaaataca tttctacccg cagcatcggc
    34441 tgcgatcaca ttgatacgac tgccgccgag agaatgggaa tctcggttgg cacggttgcg
    34501 tattcgccgg acagcgttgc ggattatgct ttgatgctga tgctgatggc catacggggt
    34561 gcaaaaccca ccatgcacgc cgtggcgcaa caagatttca gattggatcg tatccggggg
    34621 aaagaactgg gggatatgac tgtgggagtt attggaaccg gccatatcgg gcaagcggtc
    34681 gtcaaaaggc tgcggggatt tggatgccat gtgctggcct atgataacag ccgaaaaatg
    34741 gatgcagatt atgtccagct tgatgagctt ctaaaaaaca gcgatattgt tacgctccat
    34801 gtgccgcttt gtgcggatac ccgccatctg atcggtcaga agcaaattgg agagatgaag
    34861 caaggcgcat ttttaatcaa caccgcgcgc ggagcgcttg tcgataccgg ggcgctggtg
    34921 gaggcgttgg aaagcggaaa gctgggtggt gcggcactgg atgtgttgga gggcgaggat
    34981 cagtttgttt ataccgattg ctcgcagaaa gtgcttgacc atcccttttt gtcgcagctt
    35041 ttaaggatgc cgaatgtgat catcacaccc catacggcgt actacactga gcgtgtgctg
    35101 caggatacta cagaaaaaac aatcaggaat tgtctcaatt ttgaaaggag tttacagcat
    35161 gaatagaata aaagtcgcaa tcatcttcgg cggttgctcg gaggaacatg atgtgtcggt
    35221 aaaatccgca atagaaattg ctgcgaacat tgatacggaa aaattcgatc cgcactacat
    35281 cggaattaca aaaaacggtg tatggaagct atgcaagaag ccatgtacgg aatgggaagc
    35341 cgacagtctc cccgccatac tctccccgga taggaaaacg catgggctgc ttgtcatgaa
    35401 agaaagcgaa tacgaaacac ggcgtattga tgtggctttc ccggttttgc atggcaaatt
    35461 cggggaggat ggtgcgatac aggggctgtt tgtattgtct ggtatcccct atgtgggctg
    35521 tgatattcaa agctccgcag cttgcatgga caaatcactg gcctacattc ttacaaaaaa
    35581 tgcgggcatc gccgttcccg aatttcaaat tattgataaa ggtgacaagc cggaggcggg
    35641 tgcgcttacc taccctgtct ttgtgaagcc ggcacggtca ggttcgtcct ttggcgtaac
    35701 caaagtaaac ggtacggaag aacttaacgc tgcgatagaa gcggcaggac aatatgatgg
    35761 aaaaatctta attgagcaag cgatttcggg ctgtgaggtc gggtgtgcgg tcatggggaa
    35821 cgaggatgat ttgattgtcg gcgaagtgga tcaaatccgg ctgagccacg gtatcttccg
    35881 catccatcag gaaaacgagc cggaaaaagg ctcagaaaat gcgatgatta cagttcccgc
    35941 agacattccg gtcgaggaac gaaatcgggt gcaggaaacg gcaaagaaag tatatcgggt
    36001 gcttggatgc agagggcttg cccgtgttga tctttttttg caggaggatg gcggcatcgt
    36061 tctaaatgag gtcaatacca tgcccggttt tacatcgtac agccgctacc cacgtatggt
    36121 ggccgccgca ggaatcacgc ttcctgcact gattgacagc ctgattacat tggcgttaaa
    36181 gaggtgataa gtatggaaaa tggttttttg tttttagatg aaatgttgca tggcgtccgt
    36241 tgggatgcca agtacgccac atgggataac ttcacaggaa aaccggtgga tgggtatgag
    36301 gtgaatcgca tcatcggcac aaaggccgtg gcgtttgctc tgcgcgaggc acaaatccat
    36361 gcggctgcgc ttggctatgg cttgctttta tgggatggat atcggccaag aactgcggtg
    36421 gactgcttcc tgcgttgggc agcgcaaccg gaggacaatc tcacaaaaga aaaattttac
    36481 cccaatatag agcgagccga gttgattaca aagggttatg tggcctcaca atccagccat
    36541 agccgtggaa gcgcaattga tcttacgctc taccacctgg atacagggga acttgtttca
    36601 atgggaagta acttcgattt tatggacgaa cggtcgcatc atacagcaaa agggataggg
    36661 gatgcagagg cacaaaatcg aagatgcttg cgtaaaatca tggaaagcag cggatttcag
    36721 tcttatcgct ttgaatggtg gcactataag ttgattgatg agccataccc cgatacctat
    36781 tttaattttg ccgtttcata atgaaagtat tggttttgta tcagattttg gatttgataa
    36841 aatctatgct ggtcatggac aatcttttac tggaaagtgg ttaaagcatc ttttgtaata
    36901 cattgcaaat tatagtattc aatctgccca gcggcagaaa agaaaaaaac cgctgttggg
    36961 cagacctttc ccacgcccat tttgtatttt gctggcggcg gttttgagaa atcaaggccg
    37021 ccgttctttt ctggcagcaa aaggtgtggc ggcccatagg aggatgccgc tatgctgttt
    37081 tgctattgcc atgctctaat ccacttccac cactcaaaaa agccttgccc ctccacaggg
    37141 ctattccggt tatgagtatg tactatacca tgtaaatcag cagcatagca aagcattttc
    37201 tcgcccggta ggtttgcgac ctgccgggcg atttttgtcg tcttttgcgg cccggctgtc
    37261 cccaaaaaaa ttttttgaga aattttcaaa aaggaggtat ttagcgggta gctggcggcc
    37321 ttgtcaaagt cttgttagcg gaaagggaga gaaccaccta agtcccccac agaaaggggg
    37381 tgagaagatg gatgctatcc cccgcgacta tgaagcgcgg tgcatgttcg atgctttttg
    37441 caagacggta ctgcgtaacg aggccaaaag ctacttggct gaaatgaagc gccgccgtga
    37501 ccgtgaagtg tcactaagct ctttgtcaca ggcagatttg gacaagctct gtacagtaga
    37561 tcactacccc agcgacagct ttacgttttc gtcgcatggc tatgacctac atatcaacaa
    37621 tgagcttgtt gccgaagcct ttgccgcgct gcccagcatg gagcagagta ttttaatcct
    37681 gcactgtgtt ctggatatgg cagatggtga gatcggcggc cttgtgggaa tgtcccggag
    37741 tgccgttcag cggcacagga caaatacctt atcggagctg cggaaacagc taaaggcgtt
    37801 gatgccgaag ggaggttaga cgcggatgaa gaacccgaac tacaagggca ttgagctttt
    37861 gcccctttcg gtgatcgagg ccgcccgcgc tggggatgcc ggagccgtag agcgcgtttt
    37921 gcagtattac agcggttaca tgaacaagct gtgtacccgg acgctctatg acgaaaacgg
    37981 ctatccgcat gtgtgtctgg acgaggccat gaagcgccgt ctggaaatca agctcatcca
    38041 cgctatcgtc acgaactgac gaaccggccc cggcagcatg aaacggctta ccctttcccc
    38101 gtttcggtgt tttcccggtg gcctgatcgc accttgataa atatgcccgc aagataacgg
    38161 cggcgcatca tagggcatac ggacacattc tgtctgtgcc gagccggacg gctggtgcgc
    38221 catgatactt ttccctacgg gaaaagcgga gcgaggaaca ccacgccgca atgtgagcgt
    38281 ggcggcttgc gggcgacgac gtacaggcag gactatgata ctcccttata gccacagtcc
    38341 gagcgttaaa agcgtcgcag gcaatgggta gggccgcgtg agaaccacgc gggggtgaaa
    38401 gtcccgtgga gctgtgccag cagccgtccg tctatgcccc taaatcacac aaacctttga
    38461 atttgtgaaa gggggctgca ttatgaacca tgatgtgcct atttgggaaa aatacacgct
    38521 gacgattgag gaagcgtcta aatatttccg tatcggagaa aacaaattgc gtaagctggc
    38581 cgaggaaaat ccggcctctg gctgggtcat tatgaatggc aaccgcatcc agatcaaacg
    38641 caagcagttt gagaaaataa ttgacacttt ggacacaatc taatagtgaa aaagggccgc
    38701 agatatgtta taataattta taagcatata tgcggctctt tccgtcatgg aaaggagcat
    38761 gacgagatgt cagaaaaaag acgagacagc aaaaatcgcg ttttgcggtc tggagagagc
    38821 cagagaaaag acggacgata tgcttacaaa tatgttgata cctttggaaa accgcagttt
    38881 gtttactcat ggaagttagt gcctacggat aagaccccgg caggtaaacg ggaggatatt
    38941 tcactccggg aaaaggagaa agaaatccag aaagaccttg acgacgggat agacaccatc
    39001 ggcaagaaaa tgaccgtctg ccagctctac gccaagcaga tcaggcaccg gggcaatgtg
    39061 aagcacaaca ccaaactggg ccgggaacgg ctgatgcgta ttctggaaca agatcggctg
    39121 gggagctgcc cgattgacag tgtgaagctg tccgacgcaa aggaatgggc gcttcggatg
    39181 aaagaaaagg gcctttctta taagaccatc aataacgaca agcgttccct gaaagcggct
    39241 ttttacaccg ccatacagga cgattgcatc cgaaaaaacc cgtttgactt ccagcttagt
    39301 gacgttctgg acgacgatac ggaaccaaag gtgcctctta caccagcaca ggaagaaagt
    39361 tttctgtcct ttattcaagg tgataaggtt taccaaaagc attatgacgc aatcgttatt
    39421 ctgctgggga cagggcttcg catttcggaa ctgtgcggac tgacggacaa agatttggat
    39481 tttgaaaacc gggtaatcat cgtgagccac cagcttttga gaaataccgg cgtcggttac
    39541 tatattgacg aacccaagac ccaaagcggc gtcagaaaaa tccccatgaa tgaggaagtg
    39601 tatcaggcgt tccagcgagt gataaaaaac cgcaaaggtg caaagccgtt cattattgac
    39661 gggtatgcca atttcctgtt tctcaaacag aacggctatc ccatgacggc ggtagattat
    39721 ggcgggatgt ttggcaggct tgtgaagaag tacaacaaga gccatgaaga agcgttgccg
    39781 aaaacgacga cgccccatgc tatgcgtcat acattctgca cccggttagc caatgcggga
    39841 atgaacccca aagccttgca gtacattatg ggacattcca atatcaccat gacgctgaac
    39901 tattatgcac acgccacatt cgattccgca agggcagaaa tggaacggct ggcagcctga
    39961 cacaaggccg gttttactac tccatttact acttttgagg gggaaatggt gagaggtaat
    40021 aagaacattt gtaagtatct aacaatatca aaaatgccgg aaaagcccgg aaacacgggc
    40081 tataccgaca ttcaagaact tctaaaaaga taatctaaaa tcccatataa tttttcctca
    40141 aaagctgata caaagactta tattgagcct ggagtagtaa aaaagagaaa tattatcggt
    40201 agtcccatct ttcagattcc atatattgga ctagtccttt caaaaacggc atttgcatgg
    40261 attcaggggt tattctttac gattgctttt tgcctaacag gaatcacagc atggaatacc
    40321 tttgtagagt tgaaaaagaa gcggagacga acacagaaat ttttgtaaga atgttaaaaa
    40381 cataaatgta ttccagagat ccaacgactc ttctgaacat gaaacacgag aaaacactca
    40441 tgcagcttgt agatttagtc agttgttcgt ataatgtgtg aagaggagga gtagtatgcg
    40501 caatgaaatg atgcatcgtc ctgtagtcaa agaagaacta ttggatttta tgcgtaccca
    40561 gcaaaaaaaa ttaccgggtg agctaggcaa actggaagaa gaagctcatg tagcagaagt
    40621 accagttatt ccccatgaaa cagttgtatt tttgaaattt ttacttggac agcttcagcc
    40681 tgaacgaatc ttggagatcg gagcagctat cggtttttca tcaagtgtca tggccacgac
    40741 aatcggagaa aacgctcatg ttacgacaat tgatcgtttc gatgtcatga tcgagaaagc
    40801 taaaaaaaac tatgaacgtc taggactgac agataaagtg actttgcttg aaggtcaagc
    40861 agcagatatc ttgccggagt tgtctggtcc gtatgacttt atttttatgg atagtgcaaa
    40921 atcaaaatat attgaatttt tacctgaatg cttacgtttg ttaagaaaag gtggcgtttt
    40981 gatggtagat gacatcttcc aaggaggaac gatcttgctc ccagatgaag agattccccg
    41041 tggcaaacga gctatccatc gtaaattaaa tgaatttctt cgtgttgtga tggatcatcc
    41101 cgatttgaca tcgacaatct tgccattagg tgatggtgtg atcttaatga caaaggaatc
    41161 agaaacaatc gagttgtgat acatgattat ggcagcctgt agataatcga ttgaatcgag
    41221 ggctcaattc gaacttgatg taaaagtttc tatatttgta agtcaattaa aggatacagg
    41281 aaatctaaat aaacaggagt agcatgaagt caatgtagct cctgttttca attgataacc
    41341 ttagaacagc tgaaggagaa tgtctcgttt taaaagattg atttagtagt tgggccaaaa
    41401 taattcatta tttgctaatt aaataa
//
LOCUS       DACDPS010000015        40375 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.015, whole genome shotgun sequence.
ACCESSION   DACDPS010000015 DACDPS010000000
VERSION     DACDPS010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.015"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(162..2159)
                     /gene="tkt"
                     /locus_tag="IWJ07_000881"
     CDS             complement(162..2159)
                     /gene="tkt"
                     /locus_tag="IWJ07_000881"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAP9332479.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="IWJ07_000882"
     CDS             complement(2260..2511)
                     /locus_tag="IWJ07_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAP9332480.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2657..3280
                     /gene="lexA"
                     /locus_tag="IWJ07_000883"
     CDS             2657..3280
                     /gene="lexA"
                     /locus_tag="IWJ07_000883"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAP9332481.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3460..3678
                     /locus_tag="IWJ07_000884"
     CDS             3460..3678
                     /locus_tag="IWJ07_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332482.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3967..4479)
                     /locus_tag="IWJ07_000885"
     CDS             complement(3967..4479)
                     /locus_tag="IWJ07_000885"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAP9332483.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4493..6790)
                     /gene="recJ"
                     /locus_tag="IWJ07_000886"
     CDS             complement(4493..6790)
                     /gene="recJ"
                     /locus_tag="IWJ07_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAP9332484.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6932..7366)
                     /locus_tag="IWJ07_000887"
     CDS             complement(6932..7366)
                     /locus_tag="IWJ07_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAP9332485.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7376..8164)
                     /locus_tag="IWJ07_000888"
     CDS             complement(7376..8164)
                     /locus_tag="IWJ07_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9332486.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8161..9102)
                     /locus_tag="IWJ07_000889"
     CDS             complement(8161..9102)
                     /locus_tag="IWJ07_000889"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAP9332487.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9279..9866)
                     /locus_tag="IWJ07_000890"
     CDS             complement(9279..9866)
                     /locus_tag="IWJ07_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAP9332488.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10087..11400)
                     /gene="obgE"
                     /locus_tag="IWJ07_000891"
     CDS             complement(10087..11400)
                     /gene="obgE"
                     /locus_tag="IWJ07_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAP9332489.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11688..12698
                     /gene="gap"
                     /locus_tag="IWJ07_000892"
     CDS             11688..12698
                     /gene="gap"
                     /locus_tag="IWJ07_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP9332490.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12783..13718)
                     /locus_tag="IWJ07_000893"
     CDS             complement(12783..13718)
                     /locus_tag="IWJ07_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9332491.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            13904..14863
                     /locus_tag="IWJ07_000894"
     CDS             13904..14863
                     /locus_tag="IWJ07_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HAP9332492.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRK
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(14949..16043)
                     /locus_tag="IWJ07_000895"
     CDS             complement(14949..16043)
                     /locus_tag="IWJ07_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP9332493.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            16232..16993
                     /locus_tag="IWJ07_000896"
     CDS             16232..16993
                     /locus_tag="IWJ07_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9332494.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(17108..17548)
                     /gene="fabZ"
                     /locus_tag="IWJ07_000897"
     CDS             complement(17108..17548)
                     /gene="fabZ"
                     /locus_tag="IWJ07_000897"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP9332495.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            17768..18523
                     /gene="fabI"
                     /locus_tag="IWJ07_000898"
     CDS             17768..18523
                     /gene="fabI"
                     /locus_tag="IWJ07_000898"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAP9332496.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(18633..19646)
                     /locus_tag="IWJ07_000899"
     CDS             complement(18633..19646)
                     /locus_tag="IWJ07_000899"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAP9332497.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            19811..20731
                     /locus_tag="IWJ07_000900"
     CDS             19811..20731
                     /locus_tag="IWJ07_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAP9332498.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            complement(21011..21511)
                     /locus_tag="IWJ07_000901"
     CDS             complement(21011..21511)
                     /locus_tag="IWJ07_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9332499.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(21618..22136)
                     /locus_tag="IWJ07_000902"
     CDS             complement(21618..22136)
                     /locus_tag="IWJ07_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP9332500.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            complement(22137..23492)
                     /gene="rph"
                     /locus_tag="IWJ07_000903"
     CDS             complement(22137..23492)
                     /gene="rph"
                     /locus_tag="IWJ07_000903"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAP9332501.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            complement(23495..24316)
                     /gene="racE"
                     /locus_tag="IWJ07_000904"
     CDS             complement(23495..24316)
                     /gene="racE"
                     /locus_tag="IWJ07_000904"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAP9332502.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            complement(24478..25134)
                     /locus_tag="IWJ07_000905"
     CDS             complement(24478..25134)
                     /locus_tag="IWJ07_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9332503.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            complement(25150..25797)
                     /locus_tag="IWJ07_000906"
     CDS             complement(25150..25797)
                     /locus_tag="IWJ07_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9332504.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            complement(25810..26625)
                     /locus_tag="IWJ07_000907"
     CDS             complement(25810..26625)
                     /locus_tag="IWJ07_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP9332505.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            complement(26639..27376)
                     /locus_tag="IWJ07_000908"
     CDS             complement(26639..27376)
                     /locus_tag="IWJ07_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9332506.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            27630..28640
                     /gene="trpS"
                     /locus_tag="IWJ07_000909"
     CDS             27630..28640
                     /gene="trpS"
                     /locus_tag="IWJ07_000909"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAP9332507.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            complement(28810..31230)
                     /locus_tag="IWJ07_000910"
     CDS             complement(28810..31230)
                     /locus_tag="IWJ07_000910"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAP9332508.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(31235..32278)
                     /gene="pheS"
                     /locus_tag="IWJ07_000911"
     CDS             complement(31235..32278)
                     /gene="pheS"
                     /locus_tag="IWJ07_000911"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAP9332509.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            complement(32587..32928)
                     /locus_tag="IWJ07_000912"
     CDS             complement(32587..32928)
                     /locus_tag="IWJ07_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9332510.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            complement(32997..36719)
                     /gene="addA"
                     /locus_tag="IWJ07_000913"
     CDS             complement(32997..36719)
                     /gene="addA"
                     /locus_tag="IWJ07_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAP9332511.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            complement(36716..40243)
                     /locus_tag="IWJ07_000914"
     CDS             complement(36716..40243)
                     /locus_tag="IWJ07_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP9332512.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
BASE COUNT        11439 a         8671 c         7047 g        13218 t
ORIGIN      
        1 ctcaagaaac acaaactaat ccgaagaatt ctttgttttg tcatttaaat gtaacatagc
       61 cgaaaacgaa aagtaatatt gatttattct tctaacacat aaagaacagc tgtctccaac
      121 ttgcagacag ctgttcttat cagtcaaaca attgcttgga tctatgatag ttgattgtac
      181 gtattcacga cattttctac tgtaaatcca aattcttcta aaattttagt gccaggggca
      241 gatgcaccga aatgatcgat tgtaatcgtt gcgccctctg ttcctacgta gcgttcccat
      301 ccaaatgatg cagcagcttc gatagccaca cgtttcttca ctgatttagg aagtacactt
      361 tctttatatt cagaagattg tttttcaaag aggtcaaagc ttggcataga aacaacagaa
      421 acatctttgc ctttttcagc caattctttt tgagctttca cagccaaatc aacttcagaa
      481 cctgttgcaa tcaaaatacc ttctggtgtt tctccttgtg atggagaaag aacatacgcg
      541 ccttttttca ccatttcatc tgctacttct tttgtagaag gcaatactgg aaggttttga
      601 cgactcaata caaggatagt aggcgcatct gttgtctcca tcgctacttt ccaagcagca
      661 cgtgtttcat ttccgtctgc cggacgaatc acttgaacac caggcatgca gcgaacagat
      721 gctagttgtt caatcggttc gtgcgttggt ccgtcttctc cgacagcaac cgagtcatgt
      781 gttaagacgt aaacaacagg agtatgttgg attgctgcta aacgaactgc tggtcttaga
      841 taatcgacaa agacaaagaa tgttccaccg tagatacgag ttccgccatg aagttggata
      901 ccattcatag ccgaagccat tgcaaattcg cggacaccga accagatgtt gcgtccttca
      961 taatgttcag gagtgaaatc tttatctgca gccaccattg tattattgga acctgacaag
     1021 tccgctgaac caccccagaa gcttggtata gctttcgata attcttggat gacttcttta
     1081 cttgaaacac ggcttgcttg actgcttcca acttcataag ttggcaattc tgcatcccag
     1141 ttttctggta gtttgccatc aaaagcatct tcaaattgtt gtgctaattc aggataagct
     1201 tttttataat tggcaaacat ttcattccat gcatcttcag ctttttgtcc ttcttctatc
     1261 atcgtttgat ggaagcgggc agctacttct tctggaacag tgaaatctgg atactcccaa
     1321 ccataaactt ctttagccat cttgatgcca tcttcaccaa gaggagcacc atgaacagca
     1381 gatgttcctt ctttaggagc accatagccg atgactgttt ttacttcgat caatgttggt
     1441 ttgtctgttt cagcttttgc ttcttcaatc gctttagaga ttgcttctag gtcatttccg
     1501 tctttcacta agatatgttg ccagccatat gcttcatagc gagcaccaac gttttctgtg
     1561 aaagcttttg aagttggacc gtctagtgaa atatcgtttg aatcgtataa aacgatcaat
     1621 ttccctagtt tcatatgacc tgccatcgaa cttgcttctt gagatacccc ttccatcaag
     1681 tctccgtcac cgcatattgc atacgtatag tgatccatca cattaaagtt ttctttgtta
     1741 tacgttgctg caaggtgcgc ttctgccatt gccataccga cagccattgc aatcccttgt
     1801 cctaatggcc ctgtagtggc ttctacacca tctgtatgat gtacttcagg atgtcctggt
     1861 gttttagaat cccattgacg gaattgtttc aaatcatcga ttgtcacttg atagccagct
     1921 aaatgaagca agctatacaa cattgctgac ccatgtcctg ccgaaagaac gaaacggtcg
     1981 cggtcagccc agttttttga tgtcgttgga tttactttca ggtgttttgt ccataacgca
     2041 tatgccatag gtgctgcgcc catcggcaat ccaggatgtc ctgagtttgc tttttggact
     2101 gcttcaatac ttaatgtacg aattgtgtta acgccaagtt ggtcaatttt gtcaaacaaa
     2161 ataacccctc ctattaattt aggttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aacaactaga taaactaaat
     2581 aaaatacgaa cattcgtttg atttagcgct ttttatatgg tactatttat ttatcaagtg
     2641 aaagaaggtg taccttgtgg cccgacaaac tgaatcaaga caaatcgaag tcttacgata
     2701 tatccatgaa caagtaaccc aaaaaggata cccgccgact gtccgtgaaa tcggggaagc
     2761 tgttcagctt tcttctactt ctactgttca cggtcatctt gcacggcttg aaaaaaaagg
     2821 gtttatccaa agagatccga cgaaacctcg tgcaatcgag ttgacaaaac aaggtttaga
     2881 aaagattggg attcgtccaa caactattcc agtacttggc gtcgtaacag ctggagaacc
     2941 cattttagca gtagaggatg cttcagactt ctttccaatc cctcctaatc tttcttccga
     3001 agaaggcagt ctctttatgc tgacaattcg tggagatagt atgatcaatg ctgggatatt
     3061 ggatggtgac aacgtgatcg ttcggaagca agaaactgct caaaatggcg atattgtcat
     3121 tgcaatgact gccgaggacg aagcgacttg caaacgattc tataaagaaa aaaacttttt
     3181 ccgtctgcag cctgaaaacg acttgctcga accaatcatc ttagatcaag tatctatatt
     3241 aggacgagta gtaggacttt atcgtagtca tatctattaa ttgctactaa ttaaaagaaa
     3301 actttttaaa aagagtttga gacgttggtc tcaaactctt ttttcgcccg ggttcttctt
     3361 ctttcaaatt tttatcactt ccctctttac aaaccgaaca aatattcgta tactaaatat
     3421 acgaacaatt gttcgcataa attaaagagg tgagaattca tgaaatcaat gagacgtctt
     3481 ggcttagtat taactgtttt gtttttaggt attattattg gtaatttcgg catacgtcca
     3541 gctttacttg tgtttgttcc gttatttact ttgtggttca tgttgtggga tgaaaagaag
     3601 taccgtcaat ctgagcggcg cagaattcat gaagatcatt tctatcatga gccctactac
     3661 agatccaggc aatcataata aggattgact gctaattatt caaaagacaa gaagttaaac
     3721 ttttacgggg gtgtgacaaa aatcggtcac atcctttttt tcgaataaac ggtgttctaa
     3781 agccagtttt tcggtattaa gtcaaattgc ggaaaaagtg attttttaaa cttctactgg
     3841 tctttgcttg tttccattta taaaaaaata gcgaccctca aaagtcagat agagaatctg
     3901 acttttgagg gtcgctttaa aatcgcttct ttaagaataa catcaagaat agcttctttt
     3961 ttctgcttaa tattccatta atgtaatgat gtcatatcct ttgattttgt cgcgtccatg
     4021 gagatccatc aattcgatca aaaatgcgca gcctacgact acaccgccta attgttcgat
     4081 cagttcgatc gtcgctttga ttgttcctcc agtagctagc aaatcgtcac aaatcagcac
     4141 acgttgacct ggtttaatgg catccttatg caatgtcaat gtatctgttc catattctaa
     4201 tccataagtc acttcgatgg tttctcgagg taacttccct tttttgcgga ctggggcaaa
     4261 accgactcct aactcataag caacaggaca tcctacgatg aatccacggg cttctggccc
     4321 aactaccata tcaattcgtt tttcttttgc ataatttacg atttgtttag tagcttcccg
     4381 ataagcgtca ccatctgcca tcagtgggga aatatctcga aacacaattc cttttgacgg
     4441 atagtcggga atactggcaa tataatcttt taaatccact ctttgtttcc tcctaggttg
     4501 ttaaccattt ctttatggta ggaatatcgc tcaaaagcaa aaattcctca actttgattt
     4561 tattgagccg tcgctggtat aatctgctct cagtcaaagg atgactttct gggctagcga
     4621 ctttttgtaa aacaccgttc tctattgtaa caaatttcaa ttcaaaaaac acctgtatca
     4681 taaaaattaa taatttttca gggatacgca aatattgagc tattgctttt attttatgac
     4741 gtatatcaat tcgttcttga gaatggatca atttaaacaa ttttgcgtac tgttctcttg
     4801 tgcctacccc attcaaatag gcctcatcag gtgaaatccc catcagataa acacgattga
     4861 atgaataaga ccgaaaaact tctttcagat ccgctaaatc atctggacaa tccataacaa
     4921 ctagagagtt tggttttctt ttttcaatta ttgcctttac agaatctagt ccttcgaaaa
     4981 caatcgcagc ttcctggata atctcaggca cgaatttcaa cgtcgaagaa tcaaatgcta
     5041 aataaaggct accttccgac gttcggctct gttcacggaa acgttttgcc cgccaatcaa
     5101 atacttgcag accacccaca gaaaaatcag ataccatcag ctgcggtttc ttacgcccgt
     5161 tccattcatt gatcgaaagc tttcctacca catctacctg attattcagc agttcttctt
     5221 cttgagtacc aaaaccaaaa gcgaccgcat caagctgact cgatgcatcg gaaaggacaa
     5281 atttcagatg ctgttggttt gctccgattc ttttgatatt ttctgcttgg acttgtcgaa
     5341 ataaaaactg aggcaaagga ttatccgtac caaatggcgc caatagtttc agctcatcaa
     5401 ttcgctctac tgttacttca tttggcagca atacttcatc aattctcaac tcaggccctc
     5461 tcatcaagtc aatttgatga tcgactatat attggttcat cttctcctgt agtatagtga
     5521 cattttcact tggcatcgtt aaaccgactg ctgcatgatg tcctccgaag taagtaaata
     5581 aatctcgcat ctggtcaagc atttcaaaaa gattcaaggc ttcaatacta cgtccagatc
     5641 ctttagctga tccatcttct tttaaagtaa ggacaagcgt tggtttaccc gtttcattca
     5701 ttattttccc agcaacaatg cccaaaacgc cttcatgcca tcctcggttt accaatagat
     5761 ggatatgatt ctccttattg accatcgcta atgcttcttg tgttatctgc tctacgattg
     5821 cttttctttc ttcattgatc tcattcaatt tttttgcttg aacggatgct tcctcttcat
     5881 caaacgtcgc aagtaacgag acggcaggac gtggatcgcc catccgaccg attgcattta
     5941 gtctgggagc aatcgaaaaa ccgatcgttg tttcgtctgc gtccctcatt tttacgccgc
     6001 tttcttcaaa tagcgcctga agtcctatcc gttctgaatg atgaatggca tcgattccta
     6061 atgaaacaag gattcgattc tcatcagtca atgaaactaa atcggcgatc gttcctatcg
     6121 ctactaaatc caaaaattct gcaggcggtt cttccaataa tgcagaagct actttgaatg
     6181 ctactccgac tcctgccaaa tctggaaatg gatagtgccc cgctggatga cgagggtgaa
     6241 caatcgcata agcgtctggt aaattctcag gtagttcgtg atgatctgta atgatcacgt
     6301 ctacaccagc ttgctgagcg tatgctaccg catcatttcc tgctaccccg ttatcaacag
     6361 tgatgatcaa ctggattcct tcttcgatct tttcttggta taccgattgg tttggtccat
     6421 acccatgctc aaatcgattt ggcaggaata cttctacttc tgcaccaagt aattccagtg
     6481 tttctttcat taccgttgca ctggtgatcc catctgcgtc ataatcaccg taaacgagga
     6541 ttttttctcc actgattaca gcttcttgga ttcggctgat tgctttatcc atatcgtgta
     6601 tcagatacgg atcatgtaat tcttgttcct ttggatgtaa aaatcggttg atatcctttt
     6661 cctcatggta acctctttgc cacagtaact taccgacaaa tggagataat ttcagttcac
     6721 ggatcatttg gagaaaggat tcagaaggct ctgtctcgct taacttccaa tcaaaatttg
     6781 cttgtctcac tttctcactc ctaacttagt tattatagca aaggaaatca gcaaaaaaaa
     6841 gcagaaagaa agaataaaaa cagatcagat ccgctagaca gatgttcagc aggacctgat
     6901 cttttgtacg accatttatg aaatatccct attagtcaaa ataattgaag ctttgtttgg
     6961 gcgattgagt tttttgttct tccggcgctt tcaattgact gatttgtgcg gttttcgctt
     7021 gtaactccgc ctgataagca gcttccaact ccgcacgttc gtttctaaac tcgcgttgct
     7081 gttcttcttt tgcttcttca attttctttt gaagttctgt ttgatattct gacatttcct
     7141 gagtcagctg tttgatttgc tttttttgct gccatagtgt tgccgaagca gtcaaaaata
     7201 caatcagtgc accaatcagc gcagaaccga tgatcactaa gatcaatggc gcagaaaagg
     7261 aagtgaagcc aaaacttaca ggaacatttt tattgttcaa tacagcaaaa agaacaataa
     7321 tcaaaactaa aacaaatcca agaatcacat tgctttgctt tttcaattcc ttcacctact
     7381 ttttgttaaa aattccgctg gctaaataat caccaatcgt tggaaacagc gtataaaatt
     7441 ttgatgcaac ttctaaaata aacggttgat tgatttcccg ttttggatgt ctcatattgc
     7501 ggacgatcat ttttgccaac ttggcaggtt ctaaaacgat ttgtccgact ttttccaaat
     7561 agcttccgct tggatcagcc ttatcgaaga aatttgtctc gattggacct ggattcactg
     7621 tcgtaacagc tacgccaagt ggtttcaatt ctaatcgcaa agcgttggag aagccaagga
     7681 ctgcaaattt cgtggcagaa taaaccgttg attttgcagt tgccattttt ccagccatag
     7741 aggcgatatt aatgatatga ccttgtcttc tttctgccat atcgatcgca aatttttgtg
     7801 tcaaaaccat cattcctaag atgttgactt caaacatatc gtatgctttt ccaaggtcga
     7861 atgtaaggaa gtcttcgaaa atcccaaaac ccgcattgtt taccagaata tcgacgcgcc
     7921 cgacttcttc catcaccttt tcatataatt cgtccacgct ttcaggattg ctgacgtcta
     7981 ccttgaaagc ataagcctct ttgccgctca agcgtctgca ctcgtctctg acaccttcta
     8041 caaatgccat tcttcgtgcg caacttataa cgattgctcc ttgctttgct gcttcataac
     8101 agatttgtgc gcccagaccg cctgaactac ctgttactaa aacaacttta tcttctaaat
     8161 tcatgcctct tcctcctcgc gaaatggaat ctcaatgcta tcaaaatctt tcataatcgt
     8221 cgtgttttca aacacttctt gtgcttcttt ttcaagctct aaagctgctt ttcctaaata
     8281 tctcgcactg atatgagtaa gtagcagccg ttttgcctgc gcttcttttg caacttcagc
     8341 agcttgatga gtcgtggaat ggaaatagtt gtgcgccatt cttgcttcat ctttattaaa
     8401 tgtgctttca tgtaccagaa tatcgctatt ttccgcaagc acaacggaat tatgtgtttt
     8461 tctcgtatcg cctaaaatag tcacgattcg tcctttttta tctggaccta cgaaatcttt
     8521 tccattgatc gtccgcccat cttctagttg gatcgtttca ccttgcttca gtttgccgta
     8581 cagtggtcca gctggaatac ctaattcttt tagacgatcc acctgcaatt ctcctttatg
     8641 atcatgttca accacacgat aaccaaaact gtcgatccca tgatttagtt tttttgtata
     8701 aacagaaaat tgctgatcag taaaaatcgg ggctgtttca tttagctcaa tgaatttcag
     8761 cggataactt aaacgcgttt gcgaaatacc taaagagact tttatgtatt cctctatccc
     8821 ttttggcccg taaatctcta atggcgtatc cccaccttga aatgaccggc tgctgatgag
     8881 cccgggaagt ccaaaaatat gatcgccgtg taaatgtgta atgaaaattt taccgatttt
     8941 tctcggacga atagtcgttc gcaaaatctg catttgcgtg ccttcgccac agtcgaacaa
     9001 ccaaacttca ttgcgctcgt ctaaaagttt caatgctata cttgtcacat ttctatgttt
     9061 tgcaggaaca cctgcacctg ttcctaaaaa ttgtaattcc ataatctacc tgactttctt
     9121 taagttcctc tttaatttta gggtaaaaag aagggataat caaccgtaag tcgagaatgg
     9181 gttcaaatta aaaataacgt tctaaagcga tagcgttttc tttgaaaatt actataaaaa
     9241 cagcagcgaa cccgtttgag atcctctgct gttttttatt aatttgaaga tccagaactt
     9301 tcattgtcgt tggaagtttg cggatgtttg tccttccaag tcttttgtgc agcttctttc
     9361 gcatgatttt cagcttcttt cgcatcatgc acatcttttt cgacatcttc ataatccaag
     9421 cgagtaattt ctccgcctaa ttccatcatt accaattgat tcagtgggcg attgtcctct
     9481 tcatcagcaa tcaagatcag tcctgtagct ccttcaccga ttttatttcc tacaaagtcg
     9541 aacacggtct gggcatcttt tatttctttg gcatcttgtg tcgcaccgat cattcctccg
     9601 gcaaaccatc ctaataaaat ccctagcgga ccacctaata caccaaccaa cattccgatt
     9661 aatccacccg tagaagattt gttattaccg gtaaagtcga taaaatcttc tattttgaat
     9721 tgatgtgtcc cattaggatc atggctgaca actgccatct gttctccttt aatcgaacga
     9781 acagcatgta actttttgat ttctgaaaat gcctgatatg cctggctttc tacttgaaaa
     9841 tttaaaatga ttactcgttt gctcattaaa aacgcccctt tcataccttc ttgttattta
     9901 ttttaccttt aattgaaaaa taagaaaaga aaagcactct ttttttccaa taaaataggg
     9961 ctgtgacaaa agtcgtatca cagctccttt ttccgaataa acggcgttct tgaagctgat
    10021 cacttctgtc acaacctccc ttaaatctga atctatattt tttggtattt gtttttattt
    10081 gcttgcctat tcgacaaatt caaattcaaa ctcaccgata cgtacaaggt cgccatcttt
    10141 cgcaccgcgt gcccgaagag cttcatccac acccatacca cgtaattgac gagcaaaacg
    10201 catcactgtt tcatcatgat cgaaattcgt catttggaat aatttttcga tcttctcacc
    10261 agataagatc cacgttgcat ctgaatcacg atcgatttgg aactcaggac cttctggatt
    10321 gaatccatag tgtacagttt cttcttccag ttcttcttcg tacaatggga attctggcgt
    10381 tacttctaac aaatctgctg tagcattcaa taacgcgtcc aatccttgtc gagtcactcc
    10441 agaaattgga aatactggga tgtcatcagc aaattcatct tctttttcct tgttcagctg
    10501 ttctttgaat ttcactagat tttccggtgc atccggcata tccattttgt ttgccacgat
    10561 gatttgcgga cgctctagca agcgtaagtt ataagtagat aattctttgt tgatggcaag
    10621 atagtcttca taaggatctc gtccttccat cccactcata tcgatcacat gtaaaattac
    10681 tcgtgttctt tcgatatgac gaaggaattg tgtacccagc ccaacacctt gcgaagctcc
    10741 ttcaatcaag cctggcaagt ctgctgcggc aaaacttctg ccatcacttg tagtaaccat
    10801 tcctagatta ggtacgagcg tcgtgaaatg ataagcgccg atttttggac gagcagatga
    10861 aataacggat agtaaagtcg atttcccgac agatggaaaa cctacaagtc caacgtctgc
    10921 caatactttc aattctagtt cgatttttcg ctcttgtcct ggttcaccgt tttcagcaat
    10981 ttccggtgca ggattccgag gagaggcaaa acggatattc cctcgtccac cgcgtccacc
    11041 ttttgccaca actagtgttt gaccattttc aattaaatct cctaaaagag caccagtctc
    11101 cgcatctcgg acagtggttc cttgtggtac tttaacatag gtatcttctg aaccacgacc
    11161 gtgcatccct ttactcatcc cattttcacc tggctgcgct ttgaaatggc gattgaaccg
    11221 aaaatccatc aatgtacgta acccttcatc gacgatcaag atgacatcgc cgccacgacc
    11281 accgtctccg ccagccggtc cgccgtcagg aacgtatttt tcgcgacgaa atgcaaccat
    11341 tccgtctccg cccttgccag ctttcacatc gatcgttact tgatctaaaa acatggacat
    11401 aattaagtcc tcctattatt catttattta tttgactgcc tgaatttcca caataaacaa
    11461 aagtcttact tattttatcg gtgaaatctg attttgtcta tcatttcagc aaaaagattg
    11521 tttaccttct tcttttttta tcatttggta aggtttccaa ccttttttct gctaccaagt
    11581 ataatttttt gacaaaatat tctttgcgta gcagaataac agtgtattaa ttgtattaat
    11641 tgaggatgcc gtttcacagc acccctaata aaggaggaac ttttgatatg acagtaaaag
    11701 taggtattaa cggatttggg cgtattggcc gtctggcttt ccgccgcatc aaagaagtct
    11761 cagatgatat tgaagtagta gcaatcaacg acttaacaag tccaactatg ttagcacaat
    11821 tattgcaatt tgactctact catggtactt atcctggcac agttactgca acagaagact
    11881 caatcgtagt ggatggcgaa gcaactcgtg tttatgcaga acccgacgca agcaaaatcc
    11941 cttgggcaaa agaaaacggt gtagatatcg tactagaatg tactggtttt tatacatctg
    12001 aagaaaaagc aaaagcgcat ttagatgctg gtgtcaaacg agtagttatt tctgccccag
    12061 ctggtgctat gaaaaccatc gtctataacg tcaatgatga tacattagat gaaaacgaca
    12121 aaatcatctc agcaggttca tgtacaacaa actgtttagc acctatggct tatttcttaa
    12181 ataatgaatt tgggatcgaa gttggtacaa tgacaacagt tcatgcctac acttccacac
    12241 aaatgctctt agatggacct gtaaaaggcg gtaatttgcg tgctgctcgt tcagctgctg
    12301 acaatactat cccacattct actggtgctg cgaaagcaat cggcttagtt atccctgagc
    12361 tacaaggaaa attacaagga cacgcacaac gtgttccagt cgttgatggt tcattaactg
    12421 aattggtttc agtattgaaa acaaaagtaa ctgctgatca agtaaacgaa gcaatcaaaa
    12481 aacacacagt tgacaatcct tcattcgggt atgatgaccg tcaaatcgtt tctagcgatg
    12541 tcattggaac aactcaaggt tcgatctttg acccaacgca aacagaagta acaactgctg
    12601 gtgatttcca acttgttaaa actgttgcat ggtatgacaa tgagtatgga tttacttgcc
    12661 aaatgattcg cttactagaa aaattcgcta acttgtaatt tgatagatca atcgataaaa
    12721 gaagcctttg actggatcca gtcaaaggct tctttctttt catcccctac ttttaaactc
    12781 gttcaatgaa catattggct taaaaattgt tggactactt gctgatattt ttcaggatct
    12841 gtcttgtatg cttccgcatg ctcggctcct tttacaacat acttttcttt tggtcccttc
    12901 gttgcacgat agaccttatc taacatttca aatggtacaa atgtatcttc gtctccatgt
    12961 ataaagagca ttgggcgctg gtttttcttc aattgttcca ctgcatttgc ttcaccgaaa
    13021 aagtagccag cacggacttt tgtcactaga ctcgttactt gcataagcgg aaatgctgga
    13081 agactaaaca tatcgtctag ctgataagca agctcatcat gaacggaaga ataaccgcaa
    13141 tcttcaatga ttgctttcac attatctggt agtttttctc cgctagtcat cataactgtt
    13201 gctgctccca tgctgacacc gtatagaacg atttcttgcg attttccatt gttttctagc
    13261 actttgttta tccactgtac atagtcttta cgctctggcc atccaaatcc aatgtaatcc
    13321 ccttcgcttt ttccatgtcc tcttgcatca ggaacaagga cattgtaacc catatcatga
    13381 aacatctttg cgtaattggt catcgtttca gcgtttccca tatagccatg agccacaaga
    13441 gctgtttttt cacttttcgt ttcagcagga agataaatcg ctgataattt cagaccgtct
    13501 tttgaagtga tggtccaatc gcttcggttt tcttttgaac taaaccattt atgtgcatcc
    13561 gtttctttgg atgtctgtgt tgtaccaggt gtatcttcgt ctaaaaagtc tttctcagaa
    13621 ggcacaacag cataattata aaaataattc cctgcaaaca ctaacgcaac aatcagagct
    13681 aaagcgacgc ctaaaaggat tttcatccaa agtttcataa atcatccatc tcatttctct
    13741 tttttcatat aaccttgaat tagtataatc aaagaatctc tactatgcaa tgttcatttt
    13801 tataacccgc gaattgtaaa attaagttag aaaaataaaa aggcatttat ggtacactca
    13861 aattgtattt ccgacgaaag aaaacaaagg agtgtaacca taaatgacct acaaacatct
    13921 taccatagac gaactgacaa tgatagaatc atattatctt caacataata agccggttga
    13981 aatcgctaac cgaatgggac gtgctataca aactatttat aatgtagtca ataagttcaa
    14041 gcaaggcaag actgctcttg attattggca ccagtataaa gaaaataaga aaaaatgtgg
    14101 tagaaaagtc attcaattac ctgctcatga agtagattac attaaagaga aagtcactct
    14161 tggttggacg cctgacgtca ttatcgggcg aaaagaaagg tctgtttcat gcggtatgag
    14221 aacactttat cgtttatttt ctaaaggaat atttgatatt gacacactac cgatgaaagg
    14281 taaaagaaaa cccaatggcc atcaggaaaa acggggaaaa caacagtatc agcgctcaat
    14341 ccatgatcga cctgataatt atcctgattt caattctgag tttggtcacc ttgaaggtga
    14401 tacgatcgtt ggcattcatc ataaaagtgc cgtcattact ttagttgaaa gattatctaa
    14461 agtcattatc acgattaaac ccaacggccg taaggcatta gatattgaaa ctgcccttaa
    14521 tcaatggttt tctcgcttcc ctaaaaactt ctttaaatct attacgtttg actgtggaaa
    14581 agaattttct aactggaaag ccattagtaa ccaacatgat attgatatat attttgcgga
    14641 ccctggaaca ccttctcaac gcccattaaa cgagaattct aacgggattc tgcgtcgtaa
    14701 tggactgccg aaatcaatgg attttagaaa agtgaatcag acatttattt ccagtgtcag
    14761 caatcaacgt aatcatattc caagaaaatc attgaattac agaacaccaa tcgaaatatt
    14821 tttgagctat gtacaagaag cattttattc tagcttaatt tgacaaatca taacctataa
    14881 aaaaacaaaa taaaaaagca gaaataactg gctatttcta gcggattatt tctgcaataa
    14941 ctaattatct attttttatt ttcaattaat ttaaagcttg accatggttt caaaaagtca
    15001 aggaggaata attcgtcatc ggagattctt cctacaacat tgacacgtcc gtcgtttttc
    15061 atatccttca aggcgatttg tgtttctcct ttgtattgac cgtacccttc attgtcaatc
    15121 agtacatccc catgatgcat atctcgcgta ttatgaggcg ggaaatcttt atctttgtag
    15181 tatacacgag tcatcgttga acgaaggata tattctgagc gatcccctcg ataactatga
    15241 aggttatcaa acaagcagat tcgttcgttt tctgtaatac cttcttccgt atctactttc
    15301 aatgttggat aatccgcgtt aaatgcttct gccatttctt tcaattcttc ttcacttgcg
    15361 tacgcatttc caatagaaat atcatcaatc aatcctgtaa ggattaaatg cttcacttgt
    15421 gtcgcaattt caagatctcg gtggtcttcc agcgtgcaca agccatcttg cgtaggccaa
    15481 gggccaaact cagcagattg cgaattgacg aatgccatcg tattcaaatt atattttctg
    15541 aatttttctg aacaaaaaac aaagtgttcg tatcctaaac cagagtatcg atgaggataa
    15601 aaattatgag aacctaataa attatccgtg tttggcgaat aactcatgat gttgtcaacg
    15661 taattcgtgc ccgaactcat attgatctcg attttgatac caaaaggatt gcgcgtcatt
    15721 tttgcttctt ctgctcctgt gaatccgata tctaagcgga ccccgtaggc acccatctta
    15781 tggaaaaaag ataagtcatc gtaagaaatc tccaactgtt caaataacgc tggattgata
    15841 tcaaccatta cttccatgcc taggctattc gcgtaatcaa ctacttcttt aaattctgaa
    15901 agaacttttt ctttatcatc attgatctgc agcaaactgg taaaaacccg tttataccca
    15961 tacttatgag ctaaatccaa gtacgctttg tccttttcaa atgttgaccg ttctggataa
    16021 atcgagattc ctaattttcc catattacca tttcctcctg catcttttcg tttttgtatc
    16081 cacttacatt atagcaaaca caaagtaaat ggtctacacc ttttcttttt cttttaaaat
    16141 aaatgataaa ttccaataat ttaagtaggt attttccctc tattttgtta tcattaaaga
    16201 taacaaaata gtaaattgga aaggaacagt tatgaagaaa aataaagtta ttttaagtat
    16261 tagtttagca gcagcattgc tgtttgctgc tggttgttct gcaaacaaca cgacttcttc
    16321 tagttcgtct acagaaagtt caaaaagtac atctactgcg gaaagcacga ctgccagtag
    16381 cgtcgatttg aacgcattgg aattaccaca attgacgaaa gatgtcgccg aaaatgaaga
    16441 tctggtcgaa atggtgacga ataaaggcac aatcaaaatc aaattatttc ccgaacacgc
    16501 accaaaagcg gttgaaaact ttatgacaca tgcaaaagac ggttactaca acggactgac
    16561 tttccatcgc gtcatcaaag atttcatgat ccaaggtggc gatcctaaag gcgatggcac
    16621 tggaggggaa agcatttggg gagaaggatt tgaaacagag atcaacaacc acctttataa
    16681 tataagaggt gcgttatcta tggctcgctc acaagatcct aacagtaacg gcagccagtt
    16741 ctttatcgtt caaaatacag atgatatgca tgatggtctt ttaaaagacg actatcctca
    16801 agcaatcatc gacgcttata aaaacggcgg ttatccttca ctagatggcc attatactgt
    16861 cttcggccaa gtgattgaag gaatggatgt cgtggatgct attgcaaata tcgaaacaga
    16921 tagttcagac aaaccaaaag aagaagtaaa aatcgataaa atcaatatac tgcaagaagc
    16981 aaaacaaaaa taaaaaaata acgtctgaat cctgcataga tgacaacgta tgctaggatt
    17041 caggcgtctt ctttttacat aaaaagcaag taagcttcct tttgttttgc tatatcaggt
    17101 tttatagtca agcattttgg ttgacgatga acgtgaactc gcactcacag acttttttat
    17161 catctacata tgctgcagat aacgctgtac cgactgatcc cttcattttg gtgatttcaa
    17221 aatgacattt cagcacatct cctggaacaa ctttttgacg gaatttggcc ttattgatcc
    17281 caccgatata agctcttttt cctagaaact cttctgattt taggataagt atcgaacctg
    17341 cttgtgccag tgtttctaga atcaacgcac ccggcatcac tggattgtca ggaaagtgac
    17401 cttggaaaaa atcttcattg attgttacat tttttgtcgc aacgatcttt tttcctggtt
    17461 ctaaagtatc tacataatca atataacaaa taggataacg attaggaatc aattccatta
    17521 cttcagtagc agataatacg taagacataa ttaaactcct tctttcaggc aattgccatt
    17581 ttcattactt tgactatcaa agtatcatat tcctaaatat ttagcaatct ttttttaatt
    17641 taagaaaaaa tagtttgaca atcaaaacat tttgactaat aattaagaaa tcaagaagtt
    17701 attttagcta ttattctcta tttttttacg actatcaaat ttttatcaga aacaaaggag
    17761 agtcattatg tcatttctag aaggaaaaaa aatcgtcatt atgggcgtcg ctaacaaaaa
    17821 aagcattgct tggggttgtg ctgaagcaat gttgaaacaa ggggcagaaa ttatctatac
    17881 ttaccaaaat gaacgaatga aaaaatcatt agtcaaattg gtcggcgaag aagcattcac
    17941 tgtagaatgt gatgtttcta gcgacgaaag catcgaaaaa gcaatgaatg ccatcggtga
    18001 atatgctggg gagatccacg gccttgtcca tgcagttgct tacgccaaca aagaagaatt
    18061 gtccggaaat gtgtctgata tttccagaga cggctatcaa ttagctcaag atatcagcag
    18121 ctactcttta attgctgtat caaaatacgc acaaccgttt cttgcaccaa acagcgggat
    18181 cgttagcatg acttatctag gttcagaacg cgcaatcccg aattataaca tgatgggtat
    18241 tgcgaaagct tctcttgaag ctgctatccg ttatttggca gctgagttct cgcctaaagg
    18301 aattcgggtc aatggaatct cagctggggc aatcaaaaca ttagctgtca caggtgttaa
    18361 agactatcaa aaactgatcc agttatctga atctcgtacg cctgatcagc aaggtgtgac
    18421 gatcgaagaa gtcggtaatg cttgtgcttt cttaatcagc ccactttcca gcggaatcat
    18481 cggtgatatt atctacgttg acaaaggtgt ccatctttca taaacttttg ttgcattctt
    18541 taaagtaaaa catataaaaa tcaggaaaaa actttttaaa gtctcttcct gattttttct
    18601 ttttacgttt ttttcaactc attactttga aattattcca cgacttcaat ccagccttct
    18661 ggtgcttcta tgtctccaaa ttgaatacct gtcaattcat catacaaccg ttttgttact
    18721 ggtcctactt ccgtttcact ataaaataca tgaagattgt tattgtgata aatcccaccg
    18781 attggagaaa taacagccgc tgtaccacat gcaccagctt ctgcaaattg atctagatca
    18841 tcaatgaaaa tatctccttc aattgcttgc agtcctagtc gttcttcagc taactgcaac
    18901 aaggaatatt tcgtgatact tggtaagata gagggcgatt gcggagtgat gaattttccg
    18961 tcttttgtaa ttccaaagaa atttgctgca cctacttctt cgattttcgt atgtgtggct
    19021 ggatcaagat aaacacaatc tgagtaatcc agttcttttg cttctgttcc tggaagtaaa
    19081 ctcgctgcgt aatttccgcc gacttttgct gcaccagtcc ccttaggagc agcacgatcg
    19141 taatcagata caataaaatt cgttggtttt agtccacctt tgaaatacgc tcctactggc
    19201 gtacagaaga cgacaaataa atattctgga gctggatgga caccaatgtt ttcaccgacc
    19261 ccaatcagta aagggcgcaa gtataatgta gcgcctgttc catagggcgg cacaaaatct
    19321 tcatttgctt ttaccacttg cttgacagca tcaacgaaca tcttttcagg gaccattggc
    19381 atcagcaatc gtctagcact ttgattcatc cgtcgactat tttgttcagg tcgaaagaga
    19441 ttgaccttgc cgtcttttcg acgataagct tttagacctt caaaacattg ctgaccataa
    19501 tgaagcgctg gagagccttc atgaatcgtc aggtaattat cttctgtcaa ttctccttct
    19561 tcccattgtc cgtctttcca tttggcaata aaacgccaag gagttttgat ataagaaaaa
    19621 cctagattgt tccaatcgat ttgcatatac ttaccacctt cgttattttc tccacatttt
    19681 atcattatcc atattatttg tcattttatt ttcagctttt ttccttcatt atagttattt
    19741 ttgctatttc cttttttgaa aacttcttgg tacaatacag aaagaatcaa attttagaaa
    19801 agaggatttt atgttttcat tattagcaac tgccggtacg acagactctt ctggcgagac
    19861 attgacagat caagctgtca gcaaagtaaa tgcgtttcag cggttttggg acgggattga
    19921 ttgggacgcc attttggctg cctttatcca aaaaagttta tatctgattt ttttgatcgt
    19981 ccttttttgg attcttcacc gtgtgggaaa atatctgatt gataagtctt ttaatcagta
    20041 ttctaaaaaa gccattctaa acgagagccg cttaaagact ttacatagtc ttttaaatac
    20101 tatttttcat tatacccttg gtttcttttt tatctatgca attttatctg ctatcggtgt
    20161 gccgatcgga tcacttttgg ctggtgccgg aattgcaggt gtggcaatcg gtcttggtgc
    20221 ccaagggttc atgagtgatg ttattaccgg attttttatt atcatggaac aacagatgga
    20281 tgttggggac tacatcaagc tagcaaactt aacgattgaa ggaaccgtcg tatctgttgg
    20341 tatccgtacc cttcagctga aagcaacgga tggtactgtc catttcatcc cgaatcgaaa
    20401 catcaccacg atcagcaatc tctctcgtgc aaatatgcaa gtacttttag atattcggat
    20461 tgttcctgaa gaaggatatg acaaaatcta tgagatcatt gatcgagtaa atcgtacatt
    20521 agctgaaaaa taccaagaag agctccaaac tgaaccttct atctttggat tagtggatct
    20581 tggaaacagc aattttgcga ttcgtacggt ctgctacgca ttgaacggaa aacaatatgc
    20641 cttaaaagaa gagttcttga gctcctatgt caaagaattg acagcttctg gtttcacgat
    20701 tccgaacagt ccaattgctg tcggaaaata atttggacaa acgatgcaac gaatctcact
    20761 aaaaagagcg tgggacaaaa gtaaagaaca cttttgtccc acgctctaaa cacgtataaa
    20821 cggcggaagc agaagcaact ccttcggaaa taagctgaaa ttcacaaaaa tttgaaaagc
    20881 aattttcgtg aattccttct tatttcttgg agttaaacgc ttttgtccca gcctcttttt
    20941 caagataaag tcaaaactgg tacgctgttt tttctactta ttttttgatt atcttttagt
    21001 ttctcgttct ttagctcacg atatcgattt gttcttttaa ttcttcgatt ttcggagaca
    21061 atatattacg acgttctaat tcatgcacca tgaagtttac ggctttaagc agctcttttc
    21121 ttgtaatgat tcctttgaaa atccgatctt ctgtgacaac cggtaaaaaa ggcgaatcga
    21181 ctaacaaatg gagtacttct tctagttccc aattttcatc aattgtcgga acatctgttt
    21241 ctgtcacatc agcaaccgtt agtccttcta acgggtccat gctgattgtt tgcaagtcaa
    21301 tcattttatt gaccacatct gataaactta ctagtcctac aaagtgatca tcttttccta
    21361 atacaggaat tttggaataa ccgaccttcg ataacacaag taaaccatgg gacaatggat
    21421 gatgatacat aacatttgcc acattttctg caggaatcaa aaacgtttcc tgattttcca
    21481 gcagcatttc tcttactata ggtccaatca tctgatggcc tccatttcta tttcattcaa
    21541 cactactatt ttaccgaaac cggatgcaaa attcataaaa taaagcttat ttttattaaa
    21601 aattagagtt tttttattca gcgctctctc gaaaaagaaa aggccaactc ttcaactgcc
    21661 tgatgggcac ggttatagta ttgaacagaa atttggtcat ctaaactttc gataagagca
    21721 tatgatggga tttggatcgg gcctctaggc tgagaaatgc taccaggatt caaaaacagg
    21781 attttctgat ccatctcaca gcctatttga tgggtatgac caaaaaacac catatcggca
    21841 gttgcagctc gagcttcgat tgctaactgt gtcaaaccaa atcggacatt cgataaatga
    21901 ccgtgagtca tgaatactgt atcctgacca gtttggatca cttttttctg ttcgaatcct
    21961 ggtccataat cacaattacc ttgaaccact tggaatgtat cccataatgg gtcagaagct
    22021 tctaattccg aatcaccaca atggaaaaag atatccactt cagaacgata acgatctact
    22081 aagtcaacga ggatatctct gtctccatga ttatcactta ctactaaata ccgcatctat
    22141 ctctctcctt ccagccattg tttccactcg gcactcaatt ttttcattgc ttgagcccga
    22201 tgactgatct tgtttttttc ttcgctcgat aattctgctg ctgtttgttt cttaccttct
    22261 gggataaata aaggatcata cccaaagcca ttttcgccag atggtatacg ggctactcga
    22321 ccgggccatt ccgcttctac tacaaggctg tcttttttgg gtgccgcgaa tactaacgta
    22381 caatggaatt gtgctgttct tttttcatcc ggcacatctg tcaattcata taataatttg
    22441 gcgttattcc ccgcatcact tttttgttcc cctgcaaaac gtgcagaata gattcctggc
    22501 ataccgccta aagcatccac tttcaaacct gaatcatctg ctaagactgg ctgatcaagc
    22561 aattgtgcaa tcgtttctgc ttttaatcgg gcattttctt caaatgtgct gccagtttct
    22621 tccacatctg gcaattcagg atagtcaagt aatgttttta catgatatcc cgcttctttg
    22681 aacatatttc gaaactcttc tgcttttcct ggatttcttg tcgcaatgat gattgttttt
    22741 gtttcttcta tccgctcatc attttgagca aactctgtta gcaaagcatg tttttgctct
    22801 ttgatcaaat cttcaatcgc gttttttcca aaaaagagca tttcattcaa ctgttcacca
    22861 tcaaacgttg cttcttctcc agttccttga atctccacga attttccaga ttctgtcatt
    22921 acaagattca tgtctacaag tgctgcggaa tcttcttggt aatccaaatc tgtgacgcaa
    22981 gtcccatctg gtaagattcc tacgctaact gcagcaagat gttcttttat tggatcttct
    23041 gacaattctt tttctcttaa aagtttttca atggctaatt tcaatgccac aaatgcccca
    23101 gtgatacttg ctgttctagt accgccatcc gcttggatca catcgcagtc tacgatgata
    23161 ctacgttctc ctaatttttc caagtctact actgcacgca aggaacgacc aataagacgc
    23221 tggatttcca ttgttctacc agaaagtttc cctttgctgc tttctctacg atttctcgta
    23281 ctcgttgctc gaggcagcat actatattct gctgtcaccc atcctgttcc tgtttctctc
    23341 aaaaaagggg gaacagaatt ttctattgtt gcagaacaaa taactgttgt atcaccaaaa
    23401 cggatcacta ccgatccttc tggatatttg aaaacatttg tttgaagctc cagtttgcga
    23461 agctcttggg cttttctccc gtcatgacgc atccttattt tcctcccaat tcgatatgtt
    23521 ctacatttaa gtgtccgatt ccaagccagt cttcagctat ttcctcgaaa agtttggcag
    23581 agccagttgt ataaaactgg cataatgtcc gaccattttg cggtgaattg ctcaaattga
    23641 aataatctaa cagcatagat acttcaccta ctgtttctgc tccagaatcg atcagttgaa
    23701 cattttctcc cattacattt tgaatgatgg ggcgtaataa tggataatgg gtgcatccca
    23761 aaatcaatgt atcgattttt ttagtggtta aaggagctaa tgtttctgcc acaatttttt
    23821 tcgccaccga tgaatggtat tcattacttt cgacaactga aacaaatttg ggacaagcaa
    23881 gacttgtcac agtcaattct ggtactttct ctttcagtgc tttttcataa gcttgacttt
    23941 ttaccgtacc aatcgtaccg ataatgccaa cttgtttatt ttgtgttttt tttactgccg
    24001 ctctagtacc gggaaggatc acaccgatga ctggaataga aagagcagct ttgatttctt
    24061 ctaaagccac cgcagttgcg gtattgcagg cgatcaccag catcttgatt ccttgctcca
    24121 ccagataatc cgtcatttcc caagtatact ggattacctg ttccgcgggt ctagggccat
    24181 atgggcagcg tgctgtgtct cctacaaata aaatattttc attcggtaat tgtttcaggg
    24241 cttcttttac tacagtcaag ccgccgacac ctgaatcaat aaatccgata gggcgattat
    24301 ctgtcaatcg tatcatcctt tttttcaata aattctatcc tccctattgt gtacttttta
    24361 gcgtgaattt tcaagtgctc ttacttgctt aaaacaatcg aaatctcttt ttttcgaatg
    24421 gaaagaggaa aagcggttgt gactttagtt tgccacaacc gctaaaagaa tcatctatta
    24481 ataactgtac cgtctctttt ccaagtaacc ggaaagcaga gaaagcgcgt aacataccac
    24541 gaagtacata agtgtcatga tgatgaacat cggaagcaca taattggtgt tctgtccata
    24601 aataatacgt gcctgatgag aaagttcagg taaagaaatg attactgcta atgaagtatc
    24661 tttgatcagt gaaatcaatt ggctcacaat tgccgggatc attgatttaa atgcttgcgg
    24721 taacacgatc gttcgcatcg attctacata agtcagtccc gttgataatc ctgcttccat
    24781 ctgtcctttt ggaacagcat tgagtccagc tcggaaaatt tccgacaaca tagctgactc
    24841 aaaaatcgtt aatgctgcga cagcggacca gaagatgttc atctgaatgc cgatttgagg
    24901 taaggcaaag taagtgaaga aaatgatcaa taataatgga agattccgta taatatcaat
    24961 cactacacca aggattttgg aaaagaaagg gatattcgca aaacgaatca cacctgtcag
    25021 accaccgatc aagaaactaa aaatgatcga aaacaatgaa acttcaacag taacctttaa
    25081 tccttccaat aaaaaacgga tattcatcca tgaaaaagcg ccgctaaaat ccatgtattt
    25141 cccctccttc tatgaacgaa ccgcccattt tttctcaaga taaaccatta agtaagataa
    25201 cggtagcgtg ataactaagt aaaataaccc aacgatgata tacgtatcga aggtattgaa
    25261 tgtttcacta gcgatcaagt ctccttgata catcaaatct aaaccagcta cgattgctaa
    25321 aatagatgag ttttttacga ggttgataaa ttggttgcct aatggcggga taacgatttt
    25381 gaatgcttgc ggtaaaacaa tataacgcat gatttctgaa taggtaaaac cagaagatag
    25441 tcctgcttct gtttgacctt ttggtacggt ttgaatccct gaacgtactg tttcagcaat
    25501 aaaagcagat gtatagatcg ttaatccaat cgtacctgct tggaatccat ctagattaaa
    25561 ccaataaatc ggtacgacaa cataaaaaaa catcacgatg attaataatg gaatgtttct
    25621 aaaaaattct acatatgcct ttgctaaggc gctcactact ttattctttg ataattggaa
    25681 gattgccatt aacgttccga taatcaaact aaacacaagt gctaagacac tcgaaaacaa
    25741 tgtgatcttg aacccgtcga caaagattgg tccataagtt tgtatcaatt cgctcattgc
    25801 ctctccccct tactctactt ttccttcagt ttcatttgga aaccattttt gatagatttt
    25861 atcgtatgtg ccatctgcat gcatttcctc tagcgcttgg ttaacagctt tcaagaagtt
    25921 ttcttgccct ttgttgatcg caatcccgta aggctcatta gtaaacgttc caccaactaa
    25981 ctcatactct ggattttcat ctgcaatccc taacaaaatc gcattatccg tagtcatcgc
    26041 gtctccctga ccagattgta aagcagtaaa tgcttctgca tagttttcta attctaaaat
    26101 ttttgcatct ggtgcatgct gtcggatatt cgcagccgat gtagaacctt tgactgctag
    26161 aaccgttgta gaagcattca aatcatctac acttttgatt tggcttcctt tctttaccaa
    26221 caaagcttgg ccagcatcga aatagacatc tgaaaagtca acctgttttt ttcgttcatc
    26281 tgtgatcgtc atcgttgcaa taattgcatc gatatttccg tttttcaata atggaatccg
    26341 agttttagaa gtgacttcaa caaattctgt ttttccattg tcaccaagga tctttttagt
    26401 gattgctttt gcaatatcta catcaaatcc ttgtacagtt ctgctctcaa tatccatcat
    26461 tccaaataat cgagtatcat acttgacacc ccagatgatt tcattggtag atttgcttcg
    26521 ctctaaaata tcttcatttg ccaacccatt tcctttacat ccggctagtg caagaaaagc
    26581 aatgcctaaa aggaaaatca ggcgtatttt ttttaacctg cgcatctttt tcctcctatc
    26641 aatgattgat aactttactg ataaattgct gtgctcggac ttcatttgga ttttcgaaaa
    26701 agtctctcac atttctgctg tcttccaata cttctccatc agccatgaag ataacacgat
    26761 ccgccacttc tttggcaaag cccatttcat gtgtcacgac aatcattgac ataccgtcat
    26821 gagccaattt tttcataacg tctaatacat caccaatcat ttccggatcc aaagcagaag
    26881 taggttcatc aaacagcagc atctcaggat tcatcgctaa acctctggcg attgccaccc
    26941 gttgctgttg tcccccggat aacatggaag gataagaatc tttcttatct aacatattga
    27001 cagttttcaa aaatttctcc gcgtttttga ctgcagttgc ctggtcttgc ttcaacactt
    27061 tgattggtgc taatttgatg ttttccagaa cagttttatt tgggtacaaa ttgaaatgtt
    27121 ggaaaaccat gccgatgttt tttcggactt ctgtcagttt tgtcttttta tcatgcaagt
    27181 cagtatcatt aatcagcagc ttacctgaag agatcgtctc taatccattg atacagcgaa
    27241 gcatcgtgct ctttcctgaa ccagaaggac caatgacaac gacaacttct cctttctcga
    27301 attcgagatt gatatttttt aatgcgtgga acttgccata atatttatcc acgtgatcaa
    27361 atttaatcat tgccatagaa ttacactccc attattattt agtaagattt cttttaaaga
    27421 tgtataataa aatacattat atctgatagt ttagtaattt gtaaagtttc ttagaacgtt
    27481 tattctccca taaatatttc ttccatttct aatctattag ttattttatc ctctttcctt
    27541 gttttttcct taggaactgt tgtttctttt caggaaaaac ggtaagatat taagtgctca
    27601 gacttacaaa tcgtaaagag gtgtcttttt tgaaaaatat tattttaacg ggagatcgac
    27661 caactgggaa gctgcattta gggcattacg tcggttctct aaaaacccga gtggctatgc
    27721 aggcagatga agacaaccaa ctgtttgtca tgattgctga catgcaagca ctgacagata
    27781 atgctaaaaa tcctgagaag gtttcatcaa atgtcttgca agttgcttta gactatttag
    27841 ccgtgggatt agatcctgca aaatctactt tatttatcca atcacaaatt ccggaattag
    27901 ctgaattaac catgtattat ttgaacttag tcagcgtagg acgcgttcgg cgcaatccga
    27961 ctgtcaaaac agaaatcgaa caaaaaaaat tcggtgaaag cgtgcctaca ggattcttta
    28021 tctatcctgt ttctcaagca gcagatatca ctgcattcaa agcgaatctt gtacctgttg
    28081 gtgaggatca aaaaccaatg ctagagcaga cacaggaaat cgtacaaagc ttcaatcata
    28141 catatggtga agtcttggtg gaaccaaaag gcgtattccc accaaaagga atgggtagat
    28201 tacctggaat cgatggaaat ggcaaaatga gtaaatcgtt aggaaacggt atttatatct
    28261 ccgatcctgc cgatgtctta cagaaaaaag tcatgagtat gtatactgat ccaaaccaca
    28321 tccatgtaca agatcctgga caagtagaag ggaatatggt cttcacctac ttggatgtat
    28381 tcggaacaga taaagaagca attgaagaaa tgaaagctca ttaccgccgc ggcggacttg
    28441 gtgatgtaaa gatcaaacga tacctcattg atgtattgga agctgaattt gcaccgatcc
    28501 gtgcgcgccg agaagaattg gaaaaagatc cttcagctgt tatggaaatc ttacgtaaag
    28561 gtagtgaaga agctgctaaa gtagctgcac aaacactttc tgaagtaaaa tcagcaatgg
    28621 gaatcaatta cttccattaa ttcatttact ctaaaaaagc aggctgtaaa ggcctgtttt
    28681 tttatctcgt ctctgtgcta ttcctttttt aaaatcaaaa aaagagatgt gacgaagttg
    28741 tgtcacatct ttttttcaaa tgtatggtct tcaaaagcta cttgatcgtt acttactata
    28801 ttttccttct tagcgaatac ttgcctctag gttttctacc aacgcttttt cgactttttc
    28861 cattgcttta ttgatttctt catctgtcaa tgttgcttct gggttgacga atgtcaggct
    28921 atacgccatg gatttatgtc ctttttcaat gttttctcct tgatagacat caaataattg
    28981 gatatctgtt aaaaatcttc cggcagcaga acggataact tctgttaatt cctgatttgt
    29041 cacagtttct tttacaagca aagcgatgtc ccggctgact gctgggaatt ttgtcactgc
    29101 ttcgaaaacg atcccttttt gagcagcttc cactatagca gataaatcaa actcagcaac
    29161 atatgtttct ggaatatcat aactttttgt aatcgttgga tgcacttgac cgacaaaacc
    29221 aataaatttt tcgccaaggt aaatcgcagc agttcttcca ggatgcattt ccttcatttc
    29281 tgtcactgtt tgataatgga catcttcagt gatgcctagt tgttcaaata agttttctaa
    29341 aagacccttg atggtgaaaa agtctacatt ttgagctttc gtctgccaat ctttttcttc
    29401 ccaaatacca gaaatagcga ttgctgcatg ttttacttct tgcggcagat gtgtacgtgg
    29461 atcattttct tggaagaaca cccgaccaat ttcatataaa gcaatctctg tattttttcg
    29521 ggcagtgtta taactgatat catctaatag tccgctgatc aaattcattc gtaatacaga
    29581 acgttcttcg ctcattggcc agtccaaacg tgtaatatta ctttcattta taacaaactg
    29641 tcttgatttt tcttctgttg tcaatgcata actgattgct tcgctcaatc cgcttccttc
    29701 aaagattgta cgaattttac gagtagtttc ttgttctttt gacaagcttc cagcaaccgt
    29761 ttctccactt ggaagtgtag atggcaactg gtcgtatcca taaatacgag caacttcttc
    29821 gataatatct gcttcgattt ggatatccca gcgtcttggt ggtactacta cggaataaac
    29881 atactccttt tcttcatagc taaatccaag tgcttcaaaa atctcattta cttctttaac
    29941 gacaagagat gttcctaaat attgattgat gcgttcaagt gaaatcttca ctgatacgtc
    30001 ttctgctttg atctctgagc ctttgaccgc gccttgtaga acttccccac ccgcaagaga
    30061 aacgatcatt gctgcagcaa cctcactggc ttctccaaca gtcgcatggt tgattccttt
    30121 ttcaaaacgt gcagaggatt cactacgcaa attaaattgt tttgctgtac gacgaatcga
    30181 aattggatca aataaagcag attctagtgc aactgttgtt gtttcattcg tgatctctga
    30241 gtctaaccct cccatgacac cagctaaagc aactggttct ttgccgttag taatgacgag
    30301 attgtcagtt gtcaattccc gagattctcc atctagtgtt actaatgttt cattttcttt
    30361 tgcacggcgg acaaagatct cttttgattt caatttgtcg tagtcaaatg catgtagtgg
    30421 ttgtccgaat aaaagcaaaa tatagttcgt cacatctact acattgttga ttggacgaat
    30481 gccttcgttc atcaaaagat tttgcagcca ttgaggactt ggctggattc ttactccttc
    30541 aatgattctg atttgataag caggaactgc ttctttatct tcaacttcca cttgaatttt
    30601 atcgctagca ggacgtgagg attctaccag cttttcgtca tcgaatgtag gagtctgacg
    30661 ataaattgcc ccaacttcat aagctacgcc acgcattgac aatgcatccg cacgattcgg
    30721 tgtgatagat aattcaataa tcgaatcatc catatccaaa taaggaaaaa ctggatcgcc
    30781 atttacagct tcttgtggca tataatagat accttcagcg tattctttcg gtataacact
    30841 atctgaatag ccaatttctt gtaatgagca aatcatgcca tttgatactt ggccacgcat
    30901 ttttcctttt ttgattttta cattaccagc gatacgtgag ttaggaagtg caacaatcac
    30961 tttgattcca accttcacat taggcgctcc acaaacgatt tgagacagtt cgtcttcgcc
    31021 aatatcgact tgacaaatag aaaggtgatc agaatctgga tgtggtacgc attccttgac
    31081 ttcaccgaca acgatttttt tcaagccttc ttctggaacc gttactccct ctacttcgat
    31141 tcctgtcaca gacatcttat ccgctaattc ttttggtgtt acattcgata aatctacata
    31201 acgattgagc catttatatg aaactaacat tttcttactc ccccttgaac tgacttaaga
    31261 aacgtaagtc attttgatag aaattacgga tatcattcac tccataccgc aacatagcta
    31321 cgcggtctgg tccaaggccg aaagcaaagc ctgaatattc ttctggatcg attcctgaca
    31381 tcttcaacac attcgggtgt accatgccag ctcctaaaat ttcgatccat ccagtgtatt
    31441 tacatacgtt acaaccggca ccaccacatt tgaaacagct gacatctact tctactgatg
    31501 gttccgtaaa tgggaaataa cttggacgca aacgaatttc acgatcttcc ccaaacattt
    31561 ttttcattac gacttctaat gttcctttaa ggtcgcccat tgtgatgttt ttatcaacaa
    31621 ctagcccttc aatttgatgg aactgatgac tgtgggtcgc atcatctgta tctctgcgga
    31681 agactttccc tggcgagatc attcgtagag cacccttaga gaaatcatgt ttttccattg
    31741 tccgtgcttg gactggtgaa gtatgtgtcc gaatcaatat ctcgtctgaa atatagaaag
    31801 tatcttgcat gtcccttgct ggatgatctt ttggcagatt catccgttcg aaattgtaat
    31861 gatcagattc tacttcatat ccttctacga tctgatagcc catacccaca aaaatatctt
    31921 caatttcttc catgatttgt gtcacgatat gacgtgtacc atgttttact tttctaccag
    31981 gtagtgtcac gtctattgtt tcttttgcca aagctgcatt taacgcttct tcttctaata
    32041 cttgtttttt tcgctcgatc tcttctgtca aaagatctcg gatctcattt gcgaaacttc
    32101 cgacttttgg tcgctcttct ggacttagat ctttcatccc acgtaatact tctgtgatcg
    32161 gtcctttttt tcctaacgtt tcaacacgta tttgatttaa ttcatttaaa tcttgtgccg
    32221 ctgtgatttt gacgagcgtt gcttctttca agctctctaa ttgtgcttgt agagacatgt
    32281 gcttgttcct cctttaaatt ccaaatacaa aacaaaaaga cctcattcct gttaaggaac
    32341 gaggtcttcg cgttaccatc ctaatttgcg gataaatgac ttacaccgca ctcttacttc
    32401 gttaacgaca attgccggtc tgattttatc agacgaactc ggaaagtgaa cttcattttt
    32461 caaacacagg atctgctttc agtctgcgac agatctccct gaactggttt agccaaacta
    32521 ctctttttcg tcatcgttct ttacttattc gtatggacta tagtttacaa gatttttttt
    32581 acaaaatcaa ccgcattctt cctctgtgat ccatttttcg ctccaaactt gtatttgacg
    32641 catcgctttt tgcaagtctg ctcctttttc agtcaagaga tattctgaac ggctcttttc
    32701 gctacaggca aagtttcgag tgactattcc ttctttttct aattccttca agcgttcgac
    32761 taatactcga tcactgacca tcgggatttt tgccgcaagc tcaacaaagc gttgcggacc
    32821 gttttcgagt agtacatcga taatcaatcc gttccatttt tttcctaaaa tagagaatgt
    32881 tttttcaaat ttagggcata aaacgaattc tgtctgtgtt agctccatca tactcacctc
    32941 ttcccagagt atagcataat ccttacaaaa cataagtcaa gtttgctcaa ttataattat
    33001 ttgttaatgt ttataatttg tttcactcga agcaaaatta ggaaaacatt ttctactggc
    33061 ttattcaaag cttctgacat tgcttgttgg taaagtctta gctgtccttg atatttttgg
    33121 acgattgcat ctatttctgc tggatcatcc ggatgtaaaa taaaatctgt tttaaaatca
    33181 tagatattga gatgatcttt ttcttctagg tatccatcga cgattccgtg gattaatagt
    33241 tcatcttctt gatttggata gtcttgaaat acttcgtcag caggaagcag catggaaaaa
    33301 ggttgttctc gttttacgtt ttctttgttg gcaatcagct gttgaccaag ttctgtttga
    33361 aagaaccata taatagaaga aacatctact tttttcgcga ctttttcgtc tactaaccgt
    33421 ttccttacca gttcctgtaa tttttgatgg atactctctg tagttggcat ctctaatggc
    33481 agtaattgta acaaagcatg cgtagcagtt ccaacagcag ctgctgacac ttgccgatct
    33541 ttttgcaaaa attttggttc tgctagttgt tcttgcgtat aacgaaattg acgggatgct
    33601 gctttgttct gactgctttc cagagtcaaa cgtgattctt gtgtatcatc tggatcttcg
    33661 aataatcgtt tgatttcaga tacagattga taacttgtgg tttgtgaaga agcttgataa
    33721 ggatattcat atgccagacg tttttgtaac gaatctgcta ataaagtttc atcttctttc
    33781 atctgctgat ctaagttaac cagttctttt tcagcaagca actgtctttg ttcaatgatc
    33841 gattgctgat tcatccatga aatagaaaac tgtgcatttg aatgttgaag aagagaagtc
    33901 gaaatttctt cctctaagta tttctgcatt tcaggatgac ggatcaatcc gtaaccaatc
    33961 caattcatca agctgcttct gcccttcaaa cgtagcgcag gatcaaaaac taactcctca
    34021 tgatcagctg cttcagacca cgtttggatc atttgctctt tatttttata ggaaccgaca
    34081 atgaaaagct tttgttctgc ccgagtcaat cctacataca atttacgcat ttcttccgac
    34141 aacaatttgt tttgctttgc caatttgatg gcttgaaacg gtaacgtatc atataacact
    34201 cgtgtttcag gatccatata gcgaattcct gctccaagtt tttcttcaaa agcataccga
    34261 ttgcgcaagt cttgtaagtt gaactccttt gtcatatcta acaaaaatac aactgggaat
    34321 tccaagcctt tacttgcatg gatcgtcata acgcgaacag cattatcttc tattgaaatg
    34381 actggttctg ctaagtcttt atctttttct tgcatttttt caatgaaacg cacgaactgg
    34441 tataatcctc tgaaagaact ttgctcgtaa gctttggcac gatcaaccaa tgcataaaga
    34501 ttggcttgtc tctgtgcccc tgctggcaga ccgaccacat actctagata tcccgtctca
    34561 taatagatat cccataacaa gtcagtgatc gaagaacggc gagcaagttc tcgccaatgc
    34621 tctagttgct tgccaaaatg ttccagctta gctgctaatt catcttcgtt tgatgcttga
    34681 taagcaagca cagcatcata gtaagtatgt gcacggttag ctaatcgaat gctggctaat
    34741 tctggttcaa tcagacccac gatcggtgag cgcaatacag ccgcaagcgg aatatcttga
    34801 tagggattat cgattaattg tagcagcgag atcatcgtgc gaatttctgt cgcttgaaaa
    34861 tagttttgag catcattcat ctctaatgga atatccaatg ttttgaaaat ctctaaaatc
    34921 gttaagttgt tcttctttgt aggtgtcaag agaacaatgt ctttgtactc tatcggacga
    34981 cttttttttg attttttatc ataaatcata aaggaggaat caattaattg acgaattttt
    35041 agtcctgtca taaaaagttc gccttctgtt ttgtcttcca gtatatcatc cgtcggaatc
    35101 tcaatctctg attcttcctg ttctttttca tagatcatga tttccggctc aaactgttca
    35161 ttctctggaa agtctgaaaa tccttggatc agtttggctg cttcgtcata attgatttga
    35221 cccactgctg gatccattag ttgttcaaaa ataagattcg taaaagaaag gacttctttt
    35281 ctagagcgga agttctcagc caaaacgatt cgacgtccgc cttctttctt tgagaaattc
    35341 tcgtatttcc cgataaataa actaggatct gctaaacgga aagaatagat ggattgtttg
    35401 acatccccaa ccatgaacat atttcctttt gtatcatcgg gttctcttag ccagtataaa
    35461 atggcttctt gtaattggtt cacgtcttga tattcatcta ccattacttc tttgaatttt
    35521 tttcgataat gttttgaagc ttcacttggc agccatacat ctttcgtttt ttctgtgaga
    35581 atctgcaacg ctaaatgttc cagatcattg aaatccagca cacctttttc ccgtttacgc
    35641 atactgaaac cgtccatgaa gctttgcgtg acttttgtca tttcttcgac taacggtagt
    35701 gctttgtccg ttaattcttc catttggctt ggggaaacag gaaaataact tttacttatc
    35761 tgctgaatca gtttttttgc tttatccctc atgcccttga cttcattgga aatttctttc
    35821 aattctgctt tccgactgct cttaaaagtt gaaaaagtta gttgttccaa accagcataa
    35881 gcttcttcta accgatcttg cgagaacgct tcatagatat tcttgatctg ctgttgttct
    35941 ccagctactt gctcatgcat tttttctaat ccttcacctt gtgctaactg agtcatctgt
    36001 tcatataact gtacacattg gtatatatct gccaaaacta aaggacggat ttgtttttga
    36061 tataatcgag atttcgctag cccctcaggc aaacgataat tatcgcttaa ctgttctagc
    36121 catttttggg ggtcagggtt ggcacgggca aactcataaa gagaaaacac cagattcgtc
    36181 aagccgtcat ctgaacgatc attagaaaaa ttcattgtta attggaaaaa tcgttcatca
    36241 ttttctgcat aaagtgcttc tctcagctcg tcccacacat cctccttcat caagatcgtt
    36301 tctgtttcat ctgttaacat gcgaaagact ggatcaatat caattaagta ataataacgt
    36361 cgaataactg tcaggcaaaa agcatgtaag gttgagatat tcgctgtggg caataaaact
    36421 aactgcttag taaaatgctg tctacgtaca gggtcacttt cgctattgac ggattcttgt
    36481 aatgcctctt gaattctttc tttcatttct cttgcggcag cttcagtaaa tgtcacaatc
    36541 aataattcat cgatatcaaa acccattttt agtttttcaa tcactcttcg aacaagcact
    36601 gtagttttac ctgatccggc agacgcagag actaatagat tatctccttg atcaaaaatc
    36661 gcttgccact gatcatctgt gaatcgttca ttttgcggtt ttaaaggaat ttgattcact
    36721 cttcttcact ccttttcaat attctacgca gtgcttcttc cttatctatt ttctccagac
    36781 ggtggtaatt attttctttc agcatcacat caaacgtaca gatgcttctg aatggacaaa
    36841 attcgcaggc aatccgctgt ttatctttat aagctggatt tagtttgatt tctccggaag
    36901 taatcgcatc tccagctaac cgcatatttt cccgattatg cgccatgaat agttgcagtt
    36961 cttcttccgt gaaaaatttg tctttactga aactaccttt ttggtattca tctttcgcat
    37021 tttttttcac tggatagatc agtgattgtt cttttgcttc cagtgaatga tctagtactt
    37081 ctaaaagagc gggatcatcg acaaaaacgc catcataact aaattttttc aatgtattct
    37141 tctctacatc atctgtctcc gtcaatacag gattatgaac atgtaaataa tatgatcctg
    37201 ctggtttaac agccgccttt ccagtcaaac gtaccgcatc catcaatgct acgtccagat
    37261 acgtcaataa ttgcatagcc atgccataat aagcttctgt gacatcgaat gaacgtccgc
    37321 tagatttgta atcaacgacg cttagccaag tatcttcttg ttcagaagct acatcgattc
    37381 gatcgatttt ccctcgaaca tggagttttc caccattact caacggtaat tccagacctg
    37441 tgataccttt tgctccagca atctgaccaa atagaatctc tgtctgaaga ggatgcatgc
    37501 ctgtattttt gctctgtttt tgcaaagccc atgtcacttt ttgaatcgtc tgtcctaact
    37561 ggtagcggat atagttcatt cttgcagaac tatccaatat ctcaaaccga agttctccga
    37621 atacttcctg cagcactttt tcagtaaagt ctctacgctg cgcatcggac atattcacta
    37681 aggataattg gtttgaaaac agtagcttga agaaacgatc caatgattca tgataaaaat
    37741 caccagttgc tgctggagtt agaccataaa cagttcgttc tttcaatttc aaaccaaatt
    37801 gcatgaagta tttgtattca caattataaa aattctccat acgtgaaatt gaagtataaa
    37861 tctcttttcc ataaagctct tccgccattt tttctggcat cgaaacagga acatttaaat
    37921 gttcctgact ttcaaaaatc tgtaaagcaa aatttcgcca tgacgaattc agtactaatt
    37981 ctttgagctt ttgccagtgc tttggtacag gttttttttc atctaaagct aatcgataaa
    38041 tcctattgac tgtattgatt ccgcttctgt aagtccctac ataacaatta gggtcgcttt
    38101 cgatcgtcaa atgctctttt ctctcgacgg gaacagataa atattctgtg attctacgca
    38161 aataaggaga catcttcagc gaaccatctg tgtcatagct ttgtgcataa ctaagatata
    38221 atcgatctgt tccggaaaga aaaacaagat aggcttggaa aggctcaaaa gagatacttt
    38281 cctttgatgg gtcgaataaa aactgtccat ctggcaaggc ctgatttaat acttctcgtt
    38341 cttcactgga caacaatcct tttgtctctt ttcggtctgg aaacacttga tcattcaacc
    38401 ctaatgcaaa agtgacggca gcttgatttg gtcgaactaa ttccaaacga ttgattcgca
    38461 cttggtcgat tgcggtaggg attttaccat aacttagatt ttctagacca cttgtaatga
    38521 tttcttggaa cagtgaccag tcaaaagccg attcaccata aattactaca tattcatcca
    38581 acagatcaat cagcgctccc catgtctgct catggtttct tgccgcttct aagtttcctc
    38641 tgtctacttc ctgattacgc caaaacaata attgttgctg tacgccactt tggatcaaaa
    38701 attgataaaa aacatttgcg gcctcttgtc ctgttttgct tttctttatt tttttcaaaa
    38761 atactggtac agctttctga aataatcgtc taatcgtatt tgactgttct tccaaatact
    38821 gtacgtcttc aatctgttct gcttcaaaat catacgccac aaactgccag tctttttcac
    38881 gagtccaata agttccttga taattgtaag caagtgctac attttcagtg atgtccagtt
    38941 ttttcctgaa ttcatctcgc tcttgtttcc attcatcaag ttcggtttct gcttccgtcg
    39001 acagaaataa ttcagttttg aaaaaacgca taatatcact tagccgataa gaatagcggt
    39061 caagagcgaa cagactgttg atccattcga tcagcggatg attttccatc atttgttctt
    39121 catcgatata aaaaggaatt gccatttctt gaaagatcgg ccgaaggata tttccatata
    39181 aagacatatt ttttgtcagc acttggatat cacgataacg gtagttttct tcactgacta
    39241 gtcgtctgat ttcaacagct aattgtcgga cttcttcagt tggattttca gcttcccaca
    39301 aatggagatg ctcttgtccc cctattttgt gtactgccgc tttcttttgg ctagatgtct
    39361 cttcccacat tgtttgcagc agttgaagct ctgtcggacg agtctgctct ggtgcaaatc
    39421 ggtccactaa tatagggact ttccgttctc ttgcactttg aaaaagacgg aaataaagct
    39481 ttccagtctc atgaaagagc gtaagtactt ctggaagatc cgctgtataa ggacgatcca
    39541 gcaataaatc gacaaccaat tgcccctttt ccatcattgc ctgcagtaat tgatactctt
    39601 gaccattgat tcgagaaaaa ccactgacaa taaatttgac gttgttgaaa tcttgtgtct
    39661 gcatataatt tgctagtata gatagtgcat cttcatttgt tagtgcacgt tgagtcaatt
    39721 cgctgtcata agctgaaaaa atcagcttca agtctttcat tttaagttgc tggtcttctg
    39781 cattaggtga atcaccaaat aaaagcatca ggtcttctgg ttgaatattt cctgtacgca
    39841 attcactgta gagttcttgt aattgacgaa taaaccctct tttattgatt tctccgcgaa
    39901 agattgttaa ttcttcctca tttttttcta aaatctgtcg caagatcatt gcagatcctg
    39961 attctgataa ttcatttcct gtaagaagag ttgtctgttg cagcaaatac caagccagac
    40021 gataaaaact aaatacttga acgtttgttg tgctgaaagt tttttttccg cttcttcttc
    40081 gcatctccgc taaaatttct tgttcctgtt cgaatttgct gtagttggga acaaggaaaa
    40141 atacttggtt acttgcatcc tggtcgagcc aagcggatgc ttcgtcgatc aagacttgac
    40201 ggtgatcttt tttcccgttt ccaagaataa attgtaaaga catgcttttc ctccttcaac
    40261 acatgatacg gtgctccttt attatatcaa aaaatactga acatttgttc tatattcttc
    40321 aaattatatc cttttaaggt tctgatattt ctactttttt aagtatacgc aaaaa
//
LOCUS       DACDPS010000016        40080 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.016, whole genome shotgun sequence.
ACCESSION   DACDPS010000016 DACDPS010000000
VERSION     DACDPS010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.016"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            494..1201
                     /locus_tag="IWJ07_000915"
     CDS             494..1201
                     /locus_tag="IWJ07_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9332513.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(1260..1904)
                     /locus_tag="IWJ07_000916"
     CDS             complement(1260..1904)
                     /locus_tag="IWJ07_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAP9332514.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            2031..2318
                     /locus_tag="IWJ07_000917"
     CDS             2031..2318
                     /locus_tag="IWJ07_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332515.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            2426..2746
                     /locus_tag="IWJ07_000918"
     CDS             2426..2746
                     /locus_tag="IWJ07_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9332516.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            2827..4140
                     /locus_tag="IWJ07_000919"
     CDS             2827..4140
                     /locus_tag="IWJ07_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP9332517.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKVCQEIKEMNEEGKKML"
     gene            4137..4856
                     /locus_tag="IWJ07_000920"
     CDS             4137..4856
                     /locus_tag="IWJ07_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAP9332518.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYRWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            4984..5535
                     /gene="raiA"
                     /locus_tag="IWJ07_000921"
     CDS             4984..5535
                     /gene="raiA"
                     /locus_tag="IWJ07_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAP9332519.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            5757..8291
                     /gene="secA"
                     /locus_tag="IWJ07_000922"
     CDS             5757..8291
                     /gene="secA"
                     /locus_tag="IWJ07_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAP9332520.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            8417..9518
                     /gene="prfB"
                     /locus_tag="IWJ07_000923"
     CDS             join(8417..8479,8481..9518)
                     /gene="prfB"
                     /locus_tag="IWJ07_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAP9332521.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            9623..10309
                     /gene="ftsE"
                     /locus_tag="IWJ07_000924"
     CDS             9623..10309
                     /gene="ftsE"
                     /locus_tag="IWJ07_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAP9332522.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            10293..11186
                     /locus_tag="IWJ07_000925"
     CDS             10293..11186
                     /locus_tag="IWJ07_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9332523.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            11338..12786
                     /gene="cls"
                     /locus_tag="IWJ07_000926"
     CDS             11338..12786
                     /gene="cls"
                     /locus_tag="IWJ07_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP9332524.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            13123..13980
                     /locus_tag="IWJ07_000927"
     CDS             13123..13980
                     /locus_tag="IWJ07_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAP9332525.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            14095..15015
                     /gene="pstC"
                     /locus_tag="IWJ07_000928"
     CDS             14095..15015
                     /gene="pstC"
                     /locus_tag="IWJ07_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAP9332526.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            15015..15899
                     /gene="pstA"
                     /locus_tag="IWJ07_000929"
     CDS             15015..15899
                     /gene="pstA"
                     /locus_tag="IWJ07_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAP9332527.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            15914..16720
                     /gene="pstB"
                     /locus_tag="IWJ07_000930"
     CDS             15914..16720
                     /gene="pstB"
                     /locus_tag="IWJ07_000930"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP9332528.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            16736..17494
                     /locus_tag="IWJ07_000931"
     CDS             16736..17494
                     /locus_tag="IWJ07_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP9332529.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGINLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            17507..18184
                     /gene="phoU"
                     /locus_tag="IWJ07_000932"
     CDS             17507..18184
                     /gene="phoU"
                     /locus_tag="IWJ07_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAP9332530.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            18435..19453
                     /locus_tag="IWJ07_000933"
                     /pseudo
     CDS             18435..19453
                     /locus_tag="IWJ07_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723340.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
     gene            19450..20217
                     /locus_tag="IWJ07_000934"
     CDS             19450..20217
                     /locus_tag="IWJ07_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP9332531.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            20317..21885
                     /gene="liaX"
                     /locus_tag="IWJ07_000935"
     CDS             20317..21885
                     /gene="liaX"
                     /locus_tag="IWJ07_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAP9332532.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEITQVQL
                     STTTGSIYLKDTDK"
     gene            22185..22502
                     /locus_tag="IWJ07_000936"
     CDS             22185..22502
                     /locus_tag="IWJ07_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAP9332533.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            22502..22852
                     /locus_tag="IWJ07_000937"
     CDS             22502..22852
                     /locus_tag="IWJ07_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAP9332534.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            23019..23957
                     /locus_tag="IWJ07_000938"
     CDS             23019..23957
                     /locus_tag="IWJ07_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAP9332535.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            23972..24802
                     /locus_tag="IWJ07_000939"
     CDS             23972..24802
                     /locus_tag="IWJ07_000939"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAP9332536.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            24846..25871
                     /locus_tag="IWJ07_000940"
     CDS             24846..25871
                     /locus_tag="IWJ07_000940"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP9332537.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            25886..26824
                     /gene="galU"
                     /locus_tag="IWJ07_000941"
     CDS             25886..26824
                     /gene="galU"
                     /locus_tag="IWJ07_000941"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP9332538.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            26949..27389
                     /locus_tag="IWJ07_000942"
     CDS             26949..27389
                     /locus_tag="IWJ07_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAP9332539.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKATAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            27392..27994
                     /locus_tag="IWJ07_000943"
     CDS             27392..27994
                     /locus_tag="IWJ07_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP9332540.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDTKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(28053..29159)
                     /locus_tag="IWJ07_000944"
     CDS             complement(28053..29159)
                     /locus_tag="IWJ07_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP9332541.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            29369..30370
                     /gene="ccpA"
                     /locus_tag="IWJ07_000945"
     CDS             29369..30370
                     /gene="ccpA"
                     /locus_tag="IWJ07_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAP9332542.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(30707..33052)
                     /locus_tag="IWJ07_000946"
     CDS             complement(30707..33052)
                     /locus_tag="IWJ07_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP9332543.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSISDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLSKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            33331..34233
                     /locus_tag="IWJ07_000947"
     CDS             33331..34233
                     /locus_tag="IWJ07_000947"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAP9332544.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTAAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            34446..36221
                     /locus_tag="IWJ07_000948"
     CDS             34446..36221
                     /locus_tag="IWJ07_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAP9332545.1"
                     /translation="MLNTLKPLINQLTKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHFSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(36287..37873)
                     /locus_tag="IWJ07_000949"
     CDS             complement(36287..37873)
                     /locus_tag="IWJ07_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAP9332546.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            38124..38348
                     /locus_tag="IWJ07_000950"
     CDS             38124..38348
                     /locus_tag="IWJ07_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAP9332547.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            38490..>40080
                     /locus_tag="IWJ07_000951"
     CDS             38490..>40080
                     /locus_tag="IWJ07_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9332548.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIAMIPLPLLAITSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYLKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVKHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLMLDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAH"
BASE COUNT        12991 a         7073 c         8485 g        11531 t
ORIGIN      
        1 cgttagttcg agagctcttt tttttgcttt gctaccttgt tccttgaaga ttctgctgaa
       61 ttaagataga gttaagttaa ctacaagaat tggagggatc cacatgaaat ggaaagaaaa
      121 agtagacgaa gcagctgaaa agttatatga tttggtcaaa tcagaaaaat acaatattga
      181 agtgaatatt ccgaaaaaag gcggaaaagc ggtccaggta aaatcaaaac gcccaacgaa
      241 tcatacaaaa aaatggatgg ctaaaaaccg ctaataataa caaaagatcc ataagtcggc
      301 attttcatcg attttagggt cttttgttat tttctaggtt gaacatatct aaaaaattgt
      361 ttctgacaat aggagtaggg agaacgatgt gtgtctaaat cctctgttga tggatagaaa
      421 cctttatctt tttcagttgt gaatcaggta tataccagtt ataatagaag aaacgaaagg
      481 acgggtgtac gttatgtcga atcaattacc tgtatatata cagattcatg accaattaaa
      541 aaatgaaatc gaacagggta tatggaaagt cggagaccgt cttccttcag aaagagaatt
      601 agcagtgaaa ttcaatgtca gccgtatgac gttgagacaa gcaattcaaa acttggcaga
      661 tgaaggaatc ttagaaagaa agatcggttc gggtacgtat gttgcgcgag aaaaagtaca
      721 agaaaaaatg tctggtgcaa caagttttac agaaattatg gaatcacaaa atcgagtacc
      781 gtctagtcgg acaatctctt atttcttgac ttcgccaagc tcaagtgaaa tggaaaaact
      841 aaacttgagt aaagatgaca cgatcttgcg aatggaacgt attcgtttcg cagatgatgt
      901 ccctatttgt tttgaagttg ccagtgttcc acaaaagctg atcgatggct acagcaaagc
      961 agagattact gaatcgttgt atcgcacgct agaagaaaaa ggcgggaaaa agatcggtcc
     1021 agcgaatcaa acgattactg ctgtgttggc ttctgagaaa attgctgact atttagaggt
     1081 aaaacgagga gatgcagttc ttcgtttgcg ccagatttct tattttgaag acggtacgcc
     1141 gtttgagtat gtgcgtacgc aatacgtagg aaatcgtttt gagttttatt tggaaaaata
     1201 aaaaacaggt gatccctgat tccttattag aaataaaaga gaatcagaat cacctgtttt
     1261 tatatatgat cagttatcag ttgctcgtga taagaaacag aaccctcttc aaattggact
     1321 tgtccgttca gcagctcaat gatggattct ttaaagtggt cagcactttt ttcatctaca
     1381 gcacagatga cgctaacttg atcagtatat tggatttctt ttataaaaaa atgttggagt
     1441 tcaagataat tctgtaattt tcctagttgc ggatacgtga tcatcacaga gatctcctgt
     1501 tgcaaggtac cttctacgat tccaatgtca gacaatgctt gagagaccgc atgagtgtat
     1561 gctcgtatta atccgcctgt gccgagtttc gttcctccaa aatatcgagt gactactgca
     1621 gctacattga tcagttgatt ttttttgagg acttccaaca tcggtacgcc cgccgttcca
     1681 cttggttctc catcatcgct gcttcgctgg atttcatttt tttctccaat gatgaaggcg
     1741 ctgcagttat ggttcgcttt ccaatgttct ttttttattt cttgaatgaa actttttgct
     1801 tggtcctctg attctattcg tctaagagaa cagataaagc gtgatttttt gatctcaatc
     1861 tcactttgtc cattttcttt tattgtacga tagcgctcga tcatcttgtc acctcttttt
     1921 ttcattttct ttattcagta taaataaagt tctaggttgt ttcaacactg taacggttgc
     1981 gcaattgctg tagtatgcga taataaagac aaaggcaggt ggatctgggg atgcaggaac
     2041 gaatgaaaaa gtacgatgca attacgcatt atttgaagaa caatggcggc tctcaagtaa
     2101 cgttgacctt tacccaattt gacgaactgc tgtttcccag taacggactt cccaaaacag
     2161 caagggaaag tacagattgg tgggcaaatg attataaaca ccctgaaaaa ggtgcctatg
     2221 gctggataaa tgcaggctat gaagtagttg tcatcaattt ggacaaagaa tatgtcgtgt
     2281 tcaataaatt agtgaaatct agttggctat ttgattaaat aaaccgcgtt tagaaaacgt
     2341 tgacaaattt ctttcttttt atataataga gtaacagacg cgaagaaaac gtttgcatag
     2401 tttgttttaa aaaggagaga aaagtatggc taaaaaaaca atcatgcttg tatgttcggc
     2461 tggtatgagc acaagtttac tagtaacaaa aatgcaaaaa gctgctgaag caaaaggaat
     2521 cgactcggat attttcgctg tgtccgcttc tgacgcagat aacaatcttg caaataaaga
     2581 cgtggatgtg ttgttattag gcccacaagt tcgctttatg aaagctgatt ttgagaaacg
     2641 tctggaacca aaaggcattc ctttagatgt catcaatatg gctgattatg gtatgatgaa
     2701 tggcgaaaaa gtgttagatc aagcaatcac gctcataggt gaataaatag aacagtataa
     2761 ctatcgaaca agcgatccaa aaaatagatc gcttgttttt tcgtatattt ttttagggcg
     2821 aagaatatga atcatttttg gggtagaagg cttctgacga gagatattga agcaatagac
     2881 tgtgaggagg taaatttacc aaattatgag aagatcgcag cagtggaacg tttgggcaac
     2941 tggattcttt gtcatcgctg tggaatgagg acgtccgtca ttgatggaca gcttgcagat
     3001 tacggttatt tttgtacaca ttgtctatat ttagggcgat gcgacactca acaagattta
     3061 tatttgtttg atcagcctga agcaaaacct cgagaagtcg tctttttttg gacaggacag
     3121 cttactgaaa aacaaacgga aatctcaaag agaatacttt gccattcaga aaaaagacat
     3181 catcttattt gggctgtaac aggtgcaggg aaaacagaga tgctttatcc tattttagtg
     3241 gaaactttaa aagctggagg aagagtagcg atttgtacac cgcgagtcga tgtttgtaat
     3301 gaattgtttt tacgctatcg ttatgttttt ccagaagaaa aaatcacgtt gctccatggg
     3361 aatacagcaa cggcttatac ctttacttct tttgttatct gtacgattca tcagctatac
     3421 cgtttttatc ggtcctttga tttaatagtg atcgatgaga tcgatgcgtt tccttataac
     3481 ggggatgcag gattagctca tgctgtgcaa acagcgatta aaccagacgg acgattcatc
     3541 tacttaagtg ccacacctga taaaaagtta ctaaaggaaa tcaagcagac gtttgacatc
     3601 cacaaacttg ctttacgctt tcatcggcgt ttgcttcctg aacccgtctt ggttttcatg
     3661 aataattggc gacaaaaatg caagaacaag aagaagataa aatctttggt gcggatcatt
     3721 catcaattaa ttaaagaaaa ccatatcctt ttattttgtc caagtatcac gatgatgcat
     3781 cgtttgaaag ccacacttca agaggctctt ccgagatatg agatcacaga tgtttcttca
     3841 agagacaatc agcgggcgga aaaagtccag aaaatgcgag aagcaaaata tgacatactg
     3901 ttaaccacga tgatccttga acgtggagtc acatttgaaa gaatatccgt tatcgtttta
     3961 ggagcggatc atccggtttt tactaaatct tctttggtac aaatcgcagg aagagccgat
     4021 cgtaaagggc cttatacgaa cagtcaagta tactttttct atgaagaaaa aactgcagct
     4081 attcgaaaag tttgtcagga aatcaaagaa atgaatgaag aagggaagaa gatgctgtga
     4141 ggtgcagttg ctgcagtaaa gatatcttcc aaaatttaac attcagcgag attattggtg
     4201 tttcgaaata cagattacct cagacttgtt cagtttgtcg cgcacaattt catttattgg
     4261 aaacaagagg ctgttctggc tgtcaaaaac caatccattt caatctagag agtacgaaaa
     4321 agaaagtaaa gtctattccc gtttactgca gtgactgtca agaatggaag aagaaatatc
     4381 cgacctatga ttttcagcac cgtgcctttt ttgtgtacga tgaaaatatg aaggcctggc
     4441 tcaatcaata caagtttatg ggagatatcc gactagcagg cacctttgca tatagatgga
     4501 cgcagataaa aaaagaatac ccgacataca tctattgtcc aataccttta tctgaaaaaa
     4561 ggcgaacaga aagaggattc aatcaagtaa gcgagatgtt gaaagcagca agaattcctt
     4621 ttcagatgct actcaaaaga gagaagcatc tgctggctca agcaaaaaac acgcgaaagg
     4681 aaagattgga gatgcctcaa ccttttgaac ttgcagtcac cttttctaag atacaaaata
     4741 aaaaaatatt gattattgat gatgtctata caacaggaca gactcttttc cacgcagcag
     4801 attgtttgca ggtcgcacag ccgaaaatca tccgaaccat ttctttggcg agatgaatta
     4861 gatgtggaaa tttgatagcg atttaatttg caaataacct agagttcgat ataatagaat
     4921 taagaagagg gaaagagtgg tccttctcct tcttgattgt ggtagacgaa aggggtaatt
     4981 tttatgttta gatataatgt acgcggcgaa aatatcgaag taactgaagc gattcgcgac
     5041 tacgttgaga aaaaagtagg caaactagaa cgctatttca gtgacgcacc cgatgcaacg
     5101 gcgcatgtga acttaaaagt ttatacagaa aaaacagcaa aagtggaagt aacgatccca
     5161 ttaccttatc ttgtattacg tgccgaagaa acttctccag atttatacgc aagtatcgat
     5221 ttggttgttg ataaattgga acgccaaatc cgcaaattca aaacaaaaat taatcgtaaa
     5281 tcccgtgaaa cgggcatgaa tactgcagat gctgctgctt tattcaatga agaggacgaa
     5341 aacgaaaatg attctgaatt agatatcgta cgtacgaaac gcctttcatt gaaaccaatg
     5401 gatagtgaag aagcagtctt acaaatgaac atgctgggac ataacttctt tatttttgaa
     5461 gatgcagaaa caaatggtac aagtatcgtt taccgccgta aagatggaaa atacggactg
     5521 attgaaacag actgatcagc cttacttaaa taacaacgaa aacttgcagc agctactgac
     5581 agctgccgca agtttttttg agtttcctcc tcttttagat gaaatatgaa taaattatga
     5641 agttcttact ttaatacgct taagatattt caattatcta taactaatga taaaatgaaa
     5701 agtaaagtct acatagtctg gataatacac agaaggaaaa ggagattaca caacgcatgg
     5761 ccaatttgat tcgttcactt atcgaaaacg ataagaaaga actaaaacgt ttagatagca
     5821 ttgcaaaaaa agttgaagca tacgctgacc aaatggcagc attaactgat gaacaattac
     5881 aggcaaaaac tgctgaattc aagggcagat accaaaaagg agaaacatta gatcaattac
     5941 ttccggaagc ttttgcagtc gtccgagaag cagcaaaacg tgtattaggt ttatatccat
     6001 accacgtaca attgatgggt ggaatcgtgc ttcacgatgg aaatatccct gaaatgcgta
     6061 ctggtgaagg taaaaccttg accgcaacga tgccagtata tctaaatgct ctgactggcg
     6121 aaggggtaca cgtagttacc gtcaatgaat atttagcaac ccgtgactcg acggaaatgg
     6181 gcgaacttta taatttctta ggattaacag tggggttgaa tatcaactca aaatcttcgg
     6241 atgaaaaacg cgaggcttat aattgtgata tcacttatag tacgaataat gaattaggat
     6301 ttgactactt gagagacaat atggtcgttt accgccatca aatggtacaa cgtccgctga
     6361 attatgccat tgtcgatgaa gttgactcaa tcttgattga tgaagcgaga acgccgctga
     6421 tcatttcagg gcaagcagaa aaatccacag ctttatatac aagagcagac aacttcgtga
     6481 aacgtttgaa agaagaagaa gattataaaa tcgatgtaca atcaaaaaca atcagtttaa
     6541 ccgaagcagg gatcgaaaaa gcagaagaaa cattcggttt ggataactta tacgacattg
     6601 aaaatactgc attgacccat cacttggatc aagcattgag agccaactat atcatgcttt
     6661 tagatatcga ttatgttgtc caagacggaa aagtactgat tgttgaccag ttcactggtc
     6721 ggatcatgga tggacgccgt tattcagatg gacttcacca agcaatcgaa gctaaagaag
     6781 gcgtagagat cgaagacgaa acgaaaacca tggcgacgat cactttccag aactatttcc
     6841 gtatgtacaa gaaacttgcg ggaatgactg gtacagctaa aacagaagaa gaagaattca
     6901 gagaaattta taacatccaa gtataccaaa ttccgacaaa tcgtccggtt atccgtgatg
     6961 atcgtccaga tttgctttac ccaacattac aaagcaagtt ccacgctgtc gtgcaagata
     7021 tcaaagatcg ctaccgcaaa ggtcaacctg tgttagtcgg aactgttgcg gttgaaacat
     7081 ctgagttatt atcagatatg ctgaatcaag aacgtattcc tcatgaagtg ctgaatgcga
     7141 aaaatcactt taaagaagca gagatcatca tgaatgctgg tcaaaaaggt gctgtgacga
     7201 ttgctaccaa catggctggt cggggaacag atatcaagtt aggacttggg gtaagagaac
     7261 ttggtggtct agcagttatc gggacagaac gacatgaatc tcgtcgtatc gataaccagc
     7321 ttcgaggacg tgcaggacgt caaggagacc ctggtatgtc acagttctac ttgtcattgg
     7381 aagatgattt gatgaaacga ttcggttcag aacgaatcaa agctttctta gatcggatga
     7441 agatcggtga ggaagatgct gttatccaaa gcaagatgct gacgaaacaa gtagaatctg
     7501 cacaaaaacg tgtagaagga aataactatg acacacgtaa aaacgtctta caatacgatg
     7561 atgtgatgcg tgaacaacgt gaagtaatct ataaacagcg acaagaagta atcatggaag
     7621 aaaaaagctt atccaagcca ctaatgaaca tggtcaaacg gacaatcagt cgtgtagtag
     7681 atgcgcatac acaattagag aaagaaaatt ggaatttgga aagtatcatc gattttgccg
     7741 gcagtactct tgtccatgaa gatagcatta ctcttgcaga tatcgaagga aaaacagctc
     7801 aggagatcaa agagtatcta aacgaacgag cacaagaagt atttaatacc aaagcggctc
     7861 aactaaatgg tccagagcaa ctgttggaat ttgaaaaagt cgtgatcttg cgtgttgtgg
     7921 actctaaatg gacagaccat attgatgcaa tggatcaatt acgtcagtca atcggcttac
     7981 gtgcttatgg acagaacaat ccattagtcg aatatcaaac agaaggctat aagatgtttg
     8041 aagatatggt aggcgccatc gagtatgaag tcactcgaat ctttatgaaa gctgagatcc
     8101 gtcaaaacgt acagcgtgaa caggtttctc aaggaacagc ttctcactca gaagacggag
     8161 atgccaaaga aaatagtaat actagcgcga aaaaacagcc aatccatgtg aataaggtcg
     8221 gaagaaatga tccttgtcct tgtggaagcg ggaaaaaata taagaactgt catggaaaaa
     8281 atgcaaacta atttctgaca gcaggattgg tgagacaaat tcttttttta agaattcgtc
     8341 tcaccttctt tctgttattt gctgattctg ttagactaga tataatgaaa aaagtatagt
     8401 gaaagaagga aaaaacatgg aaatatccga aattcgaaat ctcctgacag aaatgcagga
     8461 aaaggtcaca agtttcagga ggtctctttg acttagatca gctggaagaa gacatcgcgg
     8521 aagcagaagc aaaaatggct gaacctggtt tttgggatga cagcgaagcc gcacaaaaag
     8581 tgatcagcga aaataacgct aataaagaaa cctatgacca attccattct cttgccgaag
     8641 aactagaaga aatcgaattg atggtggaga tgctgcaaga agaacctgat gcagcaatgc
     8701 aagaagaagc agaaacacgt atcaatgcat tagatgaaaa aatgaagacg tatgaattat
     8761 ctatgctgtt agacggacct tatgatcgaa acaatgcgat cgtcgaattg catccagggg
     8821 ctggcggaac ggagtcccaa gactggggca gcatgctttt acgtatgtat acacgttggg
     8881 cagagcagca tggctatcaa gtagaaacac tagattatca agcaggtgat gaagcaggga
     8941 tcaagagtgt gaccctgctg attaaaggac ataacgcttt tggttatcta aaatcagaaa
     9001 aaggtgttca tcgccttgta cggatttcgc cttttgactc agcgaaacga cgtcatactt
     9061 ctttttgttc ggtagatgtg atgccagagc ttgatgaaac gatcgatata gaaatcaatc
     9121 cagatgattt gaaaatcgat acgtaccgag caagtggtgc aggtggtcag cacatcaata
     9181 aaacagaatc tgctgtacgg atcactcact tgcctacagg aacagtagta gctagtcagg
     9241 cacaacgctc acaattgaaa aaccgtgagc aagcgatggg gatgttaaaa gctaagcttt
     9301 atcaattaga agtggaaaag aaagaacaag aagcagctgc acttcgagga gaacaaaaag
     9361 aaatcggctg gggttcacag atccgttctt atgttttcca tccctattct atggtaaaag
     9421 accatcggac aaacgtagag acaggaaatg tccaagcagt tatggacggt gaccttgatt
     9481 tatttattga cggctattta aaaatgcatc tacaataaga acccattgtt aaattgaaat
     9541 aaaattgtaa gattttgcaa gcatgttgaa acattgccat gctataatag ctaaagactg
     9601 aaaaagatat ggagagaacg ccatgattga aatgaaggat gtaatgaaga agtactcaaa
     9661 tggtacgacc gctatccgca atctctcgat tgtcatcgaa caaggagagt ttgtctatgt
     9721 ggttggtccg tctggagccg gtaaatctac attcatcaaa ttgatgtatc gagaagaaaa
     9781 agcttcaaaa ggaactttga acgtcgcagg tcatgatcta atagcaatca aaaaccgcga
     9841 agttccttat ctacgcagag aaatcggcgt ggtgttccaa gactataaat tattaccaag
     9901 aaaaacagta tatgaaaatg ttgcgtacgc tatgcaagtt atcggcagaa aacctcgaga
     9961 tatcaaaaaa cgagtaatgg aagtgctgga ccttgttggt ttgaaacaca aagttcgagt
    10021 ctttcctagc gaattatccg gtggggaaca gcaacgtgtc tctattgctc gggcaatcgt
    10081 gaatacgcct aaagtactta tcgcggatga accgacagga aatctggatc cagaaaactc
    10141 atgggaaatc atgaaattat tagatcggat caatgcccaa ggaactactg tcgtgatggc
    10201 tacacataac agtacgatcg taaatacgat tcggcaccga gtaattgcta tcgaaaacgg
    10261 ccgcattatt cgagatcaag cggaagggga atatggatac gatgattaga acctttttcc
    10321 gccatttatt agaaagtatc aagagtttga aacgtaacgg ctggatgaca gttgcttcag
    10381 tcagtgcggt aacgatcacg ttgactttag tcggtgtatt catggcagtt atcatgaatg
    10441 caactaaatt agctcaggat atagaaggaa acgttgatgt atctgtattc gtagatatcg
    10501 gtacaaaaga cgaagagatg aaaaagctag aaaaagaatt gaatgactta gatcatgtta
    10561 aaaaagttga attttcaagc aaagaacagg aattgaaaaa aatccaaaac gaaatgggtg
    10621 attcctggaa attgtttgaa ggtgacaata acccgttata tgatgtgtat gtagtaagtg
    10681 ctactgatcc ttcttataca aaacaagtag cagaagaagc agcagatttg aagaatgttt
    10741 ctcgttctga ttacggtgga gcttcctcag atcggatttt ccaaatcgct ggtgcagttc
    10801 ggacatgggg attagttgca gcggcattgc tgctgttcgt agcgatgttt ctcatttcga
    10861 acacgattcg gatcacgatt ttgtctagac agcgtgaaat tcaaatcatg cgtttagtcg
    10921 gtgcaaaaaa tggatatatt cgctggccgt ttttccttga aggtggctgg atcggtctga
    10981 tcggtgcgat tcttccgatt ttgatcatca catttggtta tgcatggttt ttccaattga
    11041 tcaatccatc attattacgt tcccattatt cattgattca tccacaaaat atcgtttgga
    11101 aaatcaatct tttaatggtc ggcattggtg tgatcattgg ttcacttggc tcgatcattt
    11161 ctatgagacg tttcttgaaa ttctaatacc acaatataaa taaaatgaga aagttggaca
    11221 gtttcgttcc agctttcttt ttttattggc taggagaata gaaggaagcg gcttttttcc
    11281 ctttacttta aaaaacaact tgggtattct taagttggta gaagaagagg tgaagccgtg
    11341 aaaattatcg ttgacaattt ttttacgatc cttttgatta tgaatatttt gctttccttt
    11401 atcatcgttt ttcgagaaag aaaggaaact gctcaaacat gggcatggct gctcgttttg
    11461 atgttcattc cagttgtagg atttatttta tatatttttt taggaagagg gatttccaaa
    11521 gagaaaatct ttgatttgaa gatccaaggg aaaatcggta agaacctgga aattgaagaa
    11581 gacaaacagg cattgatgcg agggctttat cctcatccgc ctactggaca agtagatgtg
    11641 aaacaattga tctatatgct cacggtattt gaaagtacgc tctatacgac taaaaatgaa
    11701 atcactttat ttacagatgg tcgtgaaaaa ttcgatgcgc tgattgaaga tatcaagcag
    11761 gcagaagacc atatccactt ccagtattat atttatcgca gtgatgcatt aggtgaagaa
    11821 gttcgagatg cattgacaga ggcggcaaga cgaggggtaa aagtcagagt ccttcttgat
    11881 gcatggggtt ccacacaagt ttcttccagc ttttttcaga atttgaaaaa agcaggcgga
    11941 gaagtcgcat ttttctttcc tctatttgtt ccgtatatta atcctcggat caattatcgc
    12001 aatcatcgta aaatcgtagt tattgatggg aaaatcggat atacaggtgg gttcaatgtt
    12061 ggaaatgaat atctaggatt ggtaaagaaa tttggttatt ggcgagacaa tcatttgcgt
    12121 atttacggag aagcggtgta taccttgcaa aaccgttttt tgatggactg gaattctcag
    12181 catacaaaag aagtaggcta ttctcccaaa ttttttccgt ccattcacac tactggtgat
    12241 atagctgttc aaatcgtgac aagcggacca gataccgaac atgaacaaat caaaatgact
    12301 tatttgaaaa tgatttcgtt agccaaacgg gagatattga tccaaacgcc ttattacata
    12361 ccggatggat cgattcatga agcactgaag ttggctcttc tttcaggcgt aaaagttcat
    12421 atccaaatac ctaacaaacc agatcatctt ctcgtttact gggcaacgta ttctttcgct
    12481 gcagagctga tcgagtatgg agcaaggatc gaaacatatg agaatggatt tattcatgca
    12541 aagacgatga ttatcgatgg tggcattgtt tcagttggct cagcaaatat cgatgttcgt
    12601 tcatttcgct tggactttga ggtcaataca ctggtttatg atgaacgaat ggcttcacgt
    12661 gtaagaaaag ctttttttga agactcgaaa atttctacgc atctcacaaa agaaatgtat
    12721 gaaaaccgag gaataatcat caagatgaaa gaaggattag caagattgat ttcgccgctc
    12781 ctttaaaaca taaaaaggac atgagaacca tcaacagatg gagatctcgt gtccttttct
    12841 aacggggcca tttctgagag tacgtcagaa atggagaaag cgtctcgcat gagcgaaatt
    12901 aactttgggg tatctcttgt tttttgggga aagcaagaaa taagctaata tatgctatta
    12961 aaagagacac atttcaaagc cagtatagca tagaaacttt taatgagata gtttttttta
    13021 caattatgag aatttatttt gttatttaat gattttgatt cgttttcatc aagtaatgaa
    13081 aatgataaaa taaacatact ttaggattaa aggagatttc taatgaaaaa acttaaatta
    13141 ttcttaccaa taataggatt agccattctt ttatcaggct gttcaaagtg gattgatcga
    13201 ggagaatcca ttacggctgt tggctcttct gctttacagc ctttagtaga aacagttgct
    13261 gaaacttacc agacagaaaa tcctggaaaa ttcatcaatg tacaaggggg cggaagcgga
    13321 actggtttgt ctcaagtaca gtcaggagca gtcgacatcg gaaactctga tttgtttgcg
    13381 gaagaaaaat cggggatcga tgcctcagca ttagtcgatc atcgagtagc agttgttggg
    13441 atcactccta ttgtgaacaa ggatattggc gtaaaagaca tcagtatgga aaacctgaaa
    13501 aaactattta caggcgagat caaaaactgg agtgaagtcg gtggaaaaga tcagccagta
    13561 gttatattga accgcgcttc aggaagcgga acccgtagca cgtttgaaaa atgggtattg
    13621 gacggagaaa cagctgttca ggcacaagaa caagattcaa gcggaatggt gcgccagatc
    13681 gttgccgaca cacccggtgc aatcagctat gttgcttttt cttatgtttc cgatgatgta
    13741 gatacgttga atgttaacgg tgtagaaccg acagatgaaa atgtgacaac gaacaaatgg
    13801 acgatttggg catatgagca tatgtacaca agagatcatc caaaaaaact gacaaaagaa
    13861 tttttagact atatcttatc tgatgaagta caagataaca tcgttggaga actaggttat
    13921 atccctgttt ctcatatgaa agtcgaacgt gattggcaag gaaatatcgt taaagagtaa
    13981 tctttacaca aaatttacaa acttagctaa acttttacat gttattaaaa ccaaattgat
    14041 gttcaatttg tacactgagt gagtatgtta aacctgatta agggggagtc atctgtggaa
    14101 gatgtgaaaa aagtattatt aacaaaatca aaacgttcaa aaatggaaca gcgtggaaaa
    14161 ttcatcagtt ttttatgtat cgcgctgatc gtgttggttg tattatctat tttttatttt
    14221 gtagctagta aaggattggc cacctttttt actgataaga tcaatgtatt tgattttctg
    14281 tttggtacag attggaatcc aagccaaatc ggagcaaatg ggaaaccaat ggtaggggca
    14341 ttgcctatga tcacaggttc gtttattgtg acctttttat ctgctatcgt tgccacgcca
    14401 tttgccattg gtgcagcagt attcatgacg gagatctctc ctaaaaacgg aacgaagatc
    14461 ttacagccgg tcatcgagct gcttgtaggt attccttctg tcgtttatgg ttttatcggt
    14521 ttatcagtta tcgtgccttt tgtcagaagt atttttggcg ggacagggtt tggtatctta
    14581 gctggtacat ttgtgttgtt cgttatgatc ttgccgactg tcacaacgat gactgttgat
    14641 gcattaaagg cagtgccacg gcactatcgg gaagcttcat tagcgcttgg tgccacaaga
    14701 tggcaaacaa tctacaaagt cgtgctacga gcagccgtgc ccgggatttt aacagcagtc
    14761 gtttttggta tggctcgtgc gtttggtgaa gccttagcta tccaaatggt catcggtaat
    14821 gcagcattga tgccaacagg acttactact cctgcttcta cattgacgtc catcttaaca
    14881 atgggaatcg gaaatacaat catgggaaca gttgaaaata atgtgttatg gtcattagcg
    14941 ctgatcttgc tattgatgtc attattcttt aatatcgtga tccgtatgat tgggaagaaa
    15001 ggagagctga aataatgaac gcaaaacgct cagataaaat tgctacaggt gttttatata
    15061 tagtttcggg gatcatcgta ttgattttag cagctttact actttatatc ttagttcggg
    15121 gaataccgca tatttcatgg tccttcttga ccgaaccttc caaagcctac caagaaggcg
    15181 gtggaatcgg gatccagcta tttaactcga tttatttgct tttgatcacg atgatcatca
    15241 gttttcctat ttctttaggt gcgggtatct atctttcaga atatgctgga aaaaactggc
    15301 taacagatgt gattcgcacg tcgattgaaa tcttgagctc gttgccttct gtcgttgtcg
    15361 gtttattcgg tttcttggtt ttcgttattc aattccagta tggtttctcg attatttctg
    15421 gtgcattggc tttgactgta ttcaacttgc ctcttttgac tagaacagtt gaggagtctt
    15481 tacaagcggt ccattacaca caacgagaag caggattggc cttaggtttg tcacgctggg
    15541 aaacagtgat aaaagtggtg gttccagaag cgacaccagg gattttgact ggtgtgatcc
    15601 taagttctgg aagaattttt ggtgaagcag ctgcattgat ctatacggct ggacaaagtg
    15661 cacctgcgtt agattttacg aactggaatc cattaagtgt ttcaagcccg atcagcattt
    15721 tccgtcaagc tgaaacatta gctgtccata tttggaagat caatacagaa ggcacgatgc
    15781 ctgatggtgt ttctgtttcg gctggcgctt ctgccgtgtt gatcattgct gttttactgt
    15841 tcaacttcgg cgctcgtgcg attggcaaac gattataccg aaaaatgact tcagcctaag
    15901 gagaagaagc aaaatgaaaa aatataactg gaatgaaaca aatgtcatca ccatgccaaa
    15961 agagaaagaa gtagcactat atacggatga tctacatgtc tggtatggcg acaatgaagc
    16021 aatcaaagga gtggatctgg aatttgaaaa aaataagatc actgcactga ttggtccttc
    16081 cggctgcgga aaatctacgt atcttcgttc gttgaatcga atgaatgatg ggatcgctaa
    16141 tacgaaaatc actgggaaaa tcatgtatca aggcgtagat attaatgcgc ctcaagtgga
    16201 tgtttatgag atgcgcaaac ggattggcat ggtttttcaa cgtcctaatc cgttcagcaa
    16261 gtcgatttat gacaatatta catttgcttt gaaacagcac ggcgaaaaaa acaaaaagaa
    16321 attagatgaa atcgttgaaa caagcttgaa acaagctgcc ttgtgggacc aagtaaaaga
    16381 cagcttggac aaaagtgcct tggctttatc tggcgggcaa cagcaacgtt tatgtattgc
    16441 tcgtgcgatc gcgatgaaac ctgatatctt gctgctagac gagccggcaa gtgctttgga
    16501 ccctatttcg acaggaacgg ttgaagaaac acttgtaaac ttgaaagaag actatacgat
    16561 catcatcgtg acgcataaca tgcaacaagc agctcgtatc agcgactata ctgccttttt
    16621 ctatctagga aaagtaatgg aatatgacaa aactaacaag atatttacca gaccaaaaat
    16681 tcaggcaacc gaagattatg tgtccggtca ttttggttaa gacggaggcc tcaacatgac
    16741 aaaagaaatt atttcatcta aagatcttca tctgtattat ggaaagaaag aagcactaaa
    16801 agggatcaat ctaagtttca atcaagggga aatcactgcg atgatcggtc cttctggttg
    16861 cgggaaatcc acttatctgc gttctttgaa ccggatgaat gacctgattc cgaatgtaac
    16921 gatcaccgga agtgttttat ataaagacaa agacatctat ggtccaaaaa cagatgttgt
    16981 tgaactaaga aaagaaatcg gcatggtatt ccagcagcca aacccatttc ctttttctat
    17041 ctatgaaaat gtcatctatg gattgaaatt aaaaggggaa aaagataaaa aagtccttga
    17101 tcaagtagtt gaagaaagcc tgaaggcagc gtctgtttgg gatgatgtga aagacaaatt
    17161 gcataaaagt gctttatctt tatccggtgg gcagcagcag cgtgtatgta tcgcacgtgt
    17221 tttggcagtt aatccggaaa tcatcttatt ggatgaacca acaagtgcgc ttgatcctgt
    17281 ttctactggg aaaatcgaaa gtatgctgtt agaattaaga gagcggtata cgatgatcat
    17341 cgtgacccat aatatgtctc aagcttcacg tatttcagat aagacggcat tctttttaga
    17401 tggtcacttg atcgaataca acgatacgaa gaagatattc atgaatccag aaaaacaaga
    17461 gacagaagat tatatttcag gtcgatttgg ataaggagga aacggaatgc tacgtacaca
    17521 atttgaagaa gaattgctga atctccataa tcagttctac gaaatgggga tgatggtgag
    17581 cagcgccgtg cacaagtcag ttcgtgcata catcaaacac gataaaatct tagcccaaga
    17641 agttatcgaa aacgatataa atatcaataa catggaaaca cgcttggaga agaaaagttt
    17701 tgaaatgatc gcattgcaac agccagttac aactgatttg cgtatgatca ttacggtcat
    17761 gaaagctagc tcggatttag aacgtatggc cgatcatgcg gtgtctgttg ctaaatccac
    17821 gattcgcgta aaagggcaga cgagaatacc ggaaatcgaa aaagagatct ccgatatgtc
    17881 cgattatgtg aaaaaaatgg tggacaacgt cctagtagct tatgtcaaaa cagacgaaaa
    17941 agacgcacgt acgatcgcca atatggatca aagagtcaat gactatttca atcgaatcta
    18001 taatgcaaca atcaaaaata tgcaggaaaa tcctgagaca gtcatcagtg gaacagacta
    18061 cttgaatgtt gccagttatc tagaacggat cggagactat gtgacaaaca tctgtgaatg
    18121 gatcgtctac ttggctacag ggaaaatcac tgagttaaat acgaaccaca atgagatttt
    18181 ctaaactgaa gctgtgatta tgccgtttgt ttaggaaaag aggttgtgaa atcagcggtt
    18241 gtgaaaaagc tgttctgcat caagtaataa gaacagtcaa acaaaaaata gctcctcata
    18301 tttttcgttt ggctggactt attaccgcag atgcaagctt ttgaacaccg tttattcggg
    18361 aaagggtttg tgacaaaaca tgtcacaaac ccttttcatc atttcataga aaggataaag
    18421 aatgcaaaaa aaaaattagc atcatcacta tattttttat ttgtgtgctt acttcatggg
    18481 ttattcagca tcaagctgct ttatatgaag tacctgtcat cgagatcgaa aaagtacgaa
    18541 taaaagagga tcatcaattg attacaggaa gactgatgaa cacggatcaa aaaggagaat
    18601 cgatttattt atcggttcct tatcaaaaaa atcaaataga aacagcaaat ataaaagcga
    18661 gacaaatgta tctagtccaa atgcatggag atgaatggca gctagtagca aaaaaaaatg
    18721 atggctggct tttcttcttc acaagtatct tcattggcgc catgttgctt attggcggaa
    18781 aacagggtct atttgcttta ggaagtctca tcatcaatat cgttttactt tacttttgtt
    18841 gctcatcttt actaaaacag aagggatttc tctattaaat atatctatcc tgttcgtcgt
    18901 tatagggatg attattagca tattggcttt aaatggatgg aatagaagca gtataatcaa
    18961 aatttcagct gcagttacga gtgttttttt agcatttttt atttgtcttt tgacaatgaa
    19021 tcattggaaa gaccaaggac tgcgctatga ggaactgaat tatctgacac gaccgtatcg
    19081 ttctgttttt ctttctagcg tactgattgg aacagctgga ggaacactcg ataccgttat
    19141 cacagtcatt gcgacattag aagaattgac taagcgacaa ccagaaataa cagcgaaaca
    19201 gttatggctt tcaggaaggc aagtgggtcg agatgtgatt ggctcgatga ccaatatcct
    19261 tttgttcgtc tatatcagtg ggtcagttcc atcgcttctt ttatatttag gaaatcaatg
    19321 gtcgttcaaa gaaacgatag aacagcattt atcattggaa tttttacgcg tcattgctgg
    19381 cagcttagga atagtattat cgatccctat ctcggtcctc ttttttctga ctgtaaggag
    19441 gctgaaacga tgaatgtact tattatactt gttgggattt tagcaatcag cgtgctactt
    19501 attggcggta cgcaaggtat gtatattctt ttaggcttat tcatcaattt agggatcttt
    19561 ttcctgttgt tattcggtta tcatcagaag tggccgatac tggcattgtc ggtcatagga
    19621 tttttattga ttgctgtggt tatcttattt tttatcaacg gttataatct aaaaatgcgt
    19681 gcagcttttg tgagtatact gatcttttta ttttgtttcc tactattgct tcctatcact
    19741 gattttttag cgatccaagg ttttacatcg gtagaactag aagaattatc tggcttagat
    19801 aagactttag cgattgattt tagattactt acgagatcgt tgcttctgat tagtcttagt
    19861 ggtgctgttt tagatgctag tgtagcaatt tctagtggga cattcgaagt atatcaggca
    19921 aatccgcaat tatcttttta ccaattgcgc cattctagtt tcgctattgc taaaaaaata
    19981 ttggcgtcaa cagtaatgac acttcttttt gcattcatgg gaagttcttt agcgttgatc
    20041 ctctggttta ttgatttaga cattcctttt cagcaagtga tcaatgaaaa atcatttgtt
    20101 ctagaatata cgatggctat tcttaccaca ttgtctgcac tacttgttct tcctttaacc
    20161 tgcgtgacta cagcatatct ctttacaaaa aagaaagaac aactcaagat ggagtaaaag
    20221 ttacaactta agtcctatga catttttact gaatcagtgc ataatatgga tgtaaggaaa
    20281 agcttgcaaa caattttgct taaggaggca atttccatga aagaaagaga acgtatttta
    20341 gatttagtga aaaaaggaat cctttcgact gaagaaggct tagatttatt agaaagcatg
    20401 gcaactgaaa aagacgaaaa gcaaataaaa aaagaagcag acaaggtaac tgctagtcat
    20461 aaagaaaaag atcaggcaag tcaattgatc gataaacttg aaaacggcga agaagaaatt
    20521 tctgagccag ttgatccaaa ggaaagagaa agacaagatc aagaaaactt agaaaagatc
    20581 ttagatgaac tagcaactga agcgaataaa acttcggcgc gtctagatga agtaaatgct
    20641 caattcgctg acaataaggc agcacgcaat gagaaacagg aagcattgat gcagctgaat
    20701 acaaaagaag aattaggcga gttgacagaa gaagaattag cacagcgtca gacgttagaa
    20761 gcagaaatca aagaattaga agctagtggc gatacactgt tagaagaaca attgaaatta
    20821 gaagcagaat taaaagatat ccgtaagaat caatggtctg agaaaaaaga aacgtttact
    20881 gataagtttg aattgccaga tgattggaaa gatcaagcaa cagatacact gaatcaagta
    20941 ggcgaaaaaa tgtcagaagc tggatcacag ttaggcaaat tcttgaagaa aacattccaa
    21001 actgtttcag aaacagtgaa tgacaacatg gaatggaaag atgtcagctt acgtgtccct
    21061 gggattgcga caacgaaatt tgagcatgaa ttttactatg aagctcctgc agcaagtatc
    21121 cttgatatca aggcagcaaa cggaaacgtg acgttaaaaa cgtgggacag caacgatgtg
    21181 aaagttgaag ctaagatcaa actttacgga aaaatgggtg ctgaaccatt tgaagcattc
    21241 tctgaacgga gccagattga ggttaatgaa gatcatatct ccttccaaat tccaaataaa
    21301 cgtgtgcgtg cagaccttgt tttctactta cctaaacgag tatacgacca cgcagctatt
    21361 aaacttctaa atggcaacat catgattgaa acattggaag ctaaagatat ttatacaaaa
    21421 tctacgaatg gtaacatcat cgtggaccaa ttaactgcaa cgatgctgga agtagaaggt
    21481 gtaaacggca atatcgatat tcgaaacggg aacatcttag attctattat cgaaacagta
    21541 aatggtacag tgacatttgg tgcgacagca gaaaaccttt ctgtgtcttt ggtaaatggt
    21601 gatgtacgtc ttacgattaa agaagataat ttgaaaaaag tagaagcaag ttcagtgaac
    21661 ggaaatgtca aagtcgcttt accagatggc atcggtttgg aaggacatgc taaaacaagt
    21721 cttggaagta tcaacagccg tctgtccaat tacgaagtag ttcgtgagaa aaaagaacgg
    21781 acaaaccaaa tgttgcaatt ccgtcgtgtt tcagatggtg aaatcacgca agtgcaactt
    21841 tcaacgacaa caggaagtat ctatttaaaa gatacagata aataaagagg ttgtgaaaga
    21901 gatgttcagc cttgagcaat aagaagcgat tttgtaaaat agcttcccct attttacaaa
    21961 attgtgcctt attgccgata ggctatctct tgaacaccgt ttaccaagag attatgaacc
    22021 tgccaattat cgccgagtca caaggaataa tttcagaaaa agctcgtcac gttttatgaa
    22081 tatggtaatt tgtgaccgag aattttgcag aaagacaccg attaagaaga aggttgtcac
    22141 aacgattttc ttcatttaaa atagtagaac agaggtgaga catgatgaaa aaaaagttga
    22201 caaaatccaa taaaaatgtt gttttgacag ggactttagc aggaatagga gaatacatcg
    22261 ggattgatcc gactgttatc cgtgtgcttt atgtattcct gagcttagtt gcttttggaa
    22321 gtccaatcat tttatacatt ttattagctt tgattatccc atctgcgcgt tctgcacgaa
    22381 caaactatgg acataacaat gcatattatg aaaaaaacaa ttatcgtgaa caaactcgtg
    22441 aacaaacaaa aaaacgcaaa gaagcggaaa aaatcgatga tgacgaatgg agtgactttt
    22501 aatgtcgtac tttcaacgta ttttagttaa tacgttaaca ttcatatcat tggctgttct
    22561 ttttcctgcg atggtccatg tgagcagtat ttggacggcc gttcttgcca gcttcgtatt
    22621 gtctgtgttg aatatgtttg tcaaaccgat tttgacgata ctgtccctgc cctttacatt
    22681 agtcacattg ggattgttta cgtttgttat caatgcagcg attcttaaaa tgacctcctt
    22741 ttttgtggga gcagaacatt ttagtttttc ttcattcggt gcagcgttgt tagtagctat
    22801 cttgatgtcg cttgtcaatg taattgtcag cgaacataac agtagaaaat aaaagaaagc
    22861 tgtgccgaac tatcggcgca gtttttttat ttagaatcag gaatgcgttt tctattacat
    22921 tggtaattaa ataaaaacgt tttttttaat tggtagtact tttggctaga caatgataaa
    22981 atgatagaga acactctaat agaaatgagg tagcatgaat gtcagaagtt gtaaaagtta
    23041 gccaattagt gaaaaaatta tctttggaaa tcgtgacagg cgacgagaaa agcttagacc
    23101 gtgtcatcgt aactggggac atctcacgcc caggactaga gttgactggt tattttaatt
    23161 attattccca taatcgttta cagctatttg gcagtaaaga aatcagtttt gcggaacgga
    23221 tgatcccaga agagcgtttg atcatcatga gaagattatg tagtgaagat atgcccgcat
    23281 ttattatttc aagaggatta ccggctccag aagaaatgat ccaggcagca aatgaaaatg
    23341 ggatagctgt actgcgttct cccatctcaa cttctcgttt attaggagaa ttatccagtt
    23401 atttagatgg tcgccttgcc cctcgaacaa gtgtccacgg tgttttagtg gatgtttacg
    23461 gattaggtgt actgattcaa ggtgacagtg ggataggaaa aagtgaaaca gcgttggagt
    23521 tgatcaaacg cggtcatcga ttgatcgcag acgatcgagt agatgtttat cagcaagatg
    23581 aattaacggt gatcggagaa ccgcctaaaa tcctggaaca tctaattgaa atccgaggaa
    23641 tcgggatcat tgatgtaatg aatctttttg gtgcgagtgc ggtacgtggc tctatgcaag
    23701 tccagttggc tgtttatctg gaagcatggg caaaagataa gaaatacgac cgtctgggct
    23761 ctgaagacac gaccgttgaa atcgcggaag tgggcattcc acaaataaaa atccctgtca
    23821 aaacaggacg aaacgttgca atcatcattg aagtagccgc gatgaatttc cgtgcacgta
    23881 caatgggctt tgacgcaacg aaaacatttg aagaaagatt aagtcgcttg attgaagaaa
    23941 actcaggcaa cgactagcaa ggagtgacaa tatgttagca caaatcaatc gagttgcttt
    24001 tgagcttgta gggattccta tctactggta tgcattgatc atcgtatcag gtatcattat
    24061 cgccatgtgg cttagcagca gagaggctgt tcgggtagga ttaaaagcag atgatgtgac
    24121 agattttatg ttgtggggat tgcctatctc cattattggc gctcgcttat attatgtttt
    24181 atttgatctt ccccagtata tagctgatcc aatccaaata ttcaatatcc gttcaggagg
    24241 cttagctatt tatggcggac tgattgcagg cgcgatcacg ctgtattttt ttactaaata
    24301 tcattttatt tctttgtgga cttttttaga tatcgctgca ccaagtgtat tattagcaca
    24361 ggcaatcggt cgttggggaa atttcatgaa ccacgaagca tatggaccag acacgacgcg
    24421 aagcttctta gaaagtctgc atttaccgca attcatcatt gataatatgt atatcgaagg
    24481 gatctatcgt caaccaacat ttctttatga atcactttgg agtttgattg gatttatttt
    24541 gcttttgttt ttacgaaaga aaccacacct tttgaaaaaa ggcgaagtcc ttttatgtta
    24601 tctaatgtgg tatagtttcg ggcgtttctt tattgaagga ttacgtacag acagtcttta
    24661 ctttttaggt gtgatccgta tttctcaatt attatctgct gtgttatttg tcggtacatt
    24721 gattctattt atttggcgca gaaaaaaagg aaatttaccg gattatgacc gctcattagg
    24781 aaaaaatcaa caaattatct gatttttaga gcgttaaaaa tgaaaaaaat ttcaggagtg
    24841 gtaaaatgaa ttatagagta gcggttttag gaccaggctc ttggggtact gctttggcac
    24901 aagtcctcgc cgaaaacgga catgacgttc ggatttgggg acatcgtgct tcacaagtag
    24961 aagaaatcaa cacccaacac acgaatcaac gttatttacc agatcattat ctacccgatt
    25021 ccatccaagc attcaccaat atggaagatg cagtaaaaga cgcagatgcg gtgctattcg
    25081 tcattccaac gaaagcaata cgttctgttg caaaagaact agtgcctaaa ttgaatacaa
    25141 agccagtgat catccatgca agtaaaggat tagaacaggg aacacataaa aggatctctg
    25201 acatcttgtt agaggaaatc ccagcagaaa aaagaaaagc tgttgttgtt ctttctggac
    25261 caagccatgc agaagaagta gcgaaacatg acatcactac gatcacagct gcaagtacag
    25321 atgcaaaagc agcaaaatat gtacaagagc tgttcatgaa taattatttt cggatctata
    25381 ccaatcctga tgtgatcggt gtagagatgg gggcagcttt gaaaaatatt attgctattg
    25441 gtgctggcgc tattcacggc ttagggtttg gcgataatgc aaaggcagca atcatgacgc
    25501 gcggattagc tgagatcagc cgtttaggtg tagcaatggg tgccaacccg ctgaccttta
    25561 tcggattaag cggagtaggc gatctagtcg ttacttgcac aagtgttcat tctaggaact
    25621 ggcgcgcagg caacttgtta ggacaagggc agcccctaga agaggtgcta gccaatatgg
    25681 gaatggtcgt tgaaggtgtg aatactaccc aagcagcgat ggaactagcc caaataatgg
    25741 aaatcgaaat gcccatcact caaacaatct atgaagtgct gtatgaacaa aaagaaatca
    25801 aaacagccgc aaaagaaatc atgttacgtg atgggaaaaa agaaaatgaa ttctcattaa
    25861 caattttttg aaggagcgaa tgtatatgaa agtcaaaaaa gcagttattc cagcagcagg
    25921 attaggaaca agatttttac cagcaacaaa agctatggca aaggaaatgt tgccaattgt
    25981 tgataaacca acaatccagt tcatcgttga agaagcgttg aaatcaggaa tcgaagacat
    26041 cttgatcgtt acaggaaaag ctaaacgacc aatcgaagat cactttgatt caaatattga
    26101 attagaaaca aacctaagtg aaaaaggaaa gaccgaatta ttgaaattag tagaagaaac
    26161 gacagatgtc aacttgcatt tcatccgtca atctcatcca aagggtttag gacatgccgt
    26221 tcttcaagca agagccttta ttggaaatga accgtttgtc gttatgctag gcgatgatat
    26281 catggaagat gaagtaccat tgacacagca attaatcgat gattacgaac gtactcatgc
    26341 ttctacgatc gccgttatgc aagttcctca tgatgaaact tcaaaatacg gaattatcaa
    26401 tccaggagaa gtattggaag atgggttata caatgtcaaa aactttgttg aaaaaccaga
    26461 tccaagcaaa gcaccaagtg atttagctat tattggacgt tacttattga ctccagaaat
    26521 ttttgatgta ttgtccgagc aacaaccagg agccggaaat gaaatccaac taactgacgc
    26581 aatcgataca ttgaataaaa cacaacgcgt atttgcccgt aaattcacag gtaaacgtta
    26641 cgatgtcggt gacaaatttg gttttatgaa aacaagtatt gaatatggtc taacacatcc
    26701 agaagtaggt gcacctttac gtgagtatat cctgtcttta ggtaaaacat tagaagcaga
    26761 aggaagcaag atcgatcata atccaaaagg aataacagaa atcaaagaag aaaagaaaaa
    26821 ataacccttt tttctaacga gcttcagctg ctaattgaat aaaaagtagt tgaagctcgt
    26881 ttttatttgt ttaaaaaaca cgaaaatgct atccttatag aaaagagaga caagaaggga
    26941 gtttgatcat gtcaggagga gaaattgcag gattgattgc agcaatcgcc tttgcgatac
    27001 ttgtcgtttt tatcgttcgt gtgttgttgc aagtttctaa aactttagaa aaagtagatc
    27061 agactgtagc agaagcaaat acgacgatcg aaatcgtcac aaaagacgta gatcttttgt
    27121 ctagacaagt agaaggcttg ctggtcaaaa gtaatgaact gctcttggat atcaatggaa
    27181 aagtcgcaac gatcgatcca ttgttcacag cagttgctga tttaagtgaa agtgtatcag
    27241 aacttaatca ttctagtaaa aacatcgcga cgaaagtcgg cggaatcggt aaagcgacag
    27301 ctaaagcgac ggttgccgga aaagtcggtg ggacagcgat gaaatttttt aaaaacaaaa
    27361 aacatactga aacgacagga ggaaaataaa aatggctaaa aaaggtggat ttttattagg
    27421 tgcagtagtc ggaggaacag ccgcagcaat cgccgcgtta ttgttagcac caaaatctgg
    27481 taaagaactt cgggatgaat tagctgccca agcggatgat ttgatggata tggcttcaga
    27541 ttacacagat atggcgaaag aaaaatcgac tgagttaaca ggtattgcaa aagaaaaagc
    27601 gcaagatctt tctcaacaag caggtgatct agcaggtagt gtcaaacaaa aagtaagtga
    27661 atcagcagat gaagcaggaa gtgaatctga cgatatcttg ttaaatctta aagaacaatc
    27721 agctgactta tctaatcgtt tcaaaaaaac ggcagaagat atgaaaaaac ctgcagatga
    27781 attaggaagt attgctaaag atacttcaga agatatcatg attgatgtaa aagacgctgc
    27841 tggtaaagca ggagatactg tcaatgaagg aatgtcagaa gcaaaaccag ttgcagatga
    27901 cacaaaagaa atgatgaaaa tggcaaaaga agatgcgaaa gaagctaaaa aagaaataaa
    27961 agatacaatg acagattcat ctacaaatca ctaaaatcac aaatttatag aataaaaagg
    28021 tgggagcgga ctagctctca ccttttttac tcttattttt ctaaaacaag taattctttt
    28081 ggtgtagtcg tgaatggcac acagccatcg cttgtcacat atacacagtc ttcgatacgt
    28141 acccctactt tttcaggaat atagattcct ggttcaagag agaagcacat cccttcttcg
    28201 attactaagt catttccttc tacaagggaa gggaattcgt ggacggttgt tccgatcccg
    28261 tgacctaaac ggtgattgaa atattcgccg tagccatagc tgttgatcac gtttcgtgcg
    28321 atttgatcaa gttcacctgc tgtcacacct ggacggacgg cttcttgtgc ggcaagctgt
    28381 gcttcaagag tgatgttgta tattttttct tggaagtcgg ttggtttttg ataagcaatt
    28441 gtacgagtag catcactgca atagccattc caaacaacgc caagatcgaa taacacaagt
    28501 tcattcggtg aaatcttcgt gtttcctggt gtaccatgag ggcttgcagc atttgctcct
    28561 gcaaggacta atgtgtcgaa agacatttgt gaaacacctt gttttttcaa ttggtattcg
    28621 atttctgcaa tgatcgcttg ttcagtcacg ccttctttca ctgctttaaa gccaatctca
    28681 aatgcaacat cggcccattt accagcttcg atcagtttgt ctttttcctc tagagttttg
    28741 atcagttgta gacgttggat gatggaagtt agatcagtgt aagaatccgt tcccgtcaag
    28801 atatcagaga tagcatcgaa gcgctcgaca gtcaaggcgt tcttttcaat ccctacattg
    28861 ccaggctgac catagcgagc acgaagttct tttccgatga tctcccaagg gttttcgctg
    28921 tctaaatagc cacggacatc atacgcccaa ctactgtttt gagcatcttc aacttccaaa
    28981 gcaggagtga aaaggaaagg atcttgttca agagggataa aaagagctag tacacgctca
    29041 tgaggatcac tttcgtatcc ggaaaagtaa gcgatgtggc cagggtcgct gatataagcc
    29101 aattcgactt ggtgttgtgc catccattga cgtagttcat tgatttttac gttattcatt
    29161 tggcaaccta ctttctaaat taattttgta ttcaataatt gtaccactga tttattaaaa
    29221 gaccaacgtt ctaaaaactg aaaaaagtaa aaattttcat gaaaacagag ataaaacggt
    29281 tgcaattctt gagattattt gatattctat tttagaaatg taagacgtat tttcagaaaa
    29341 catataaaat atgaatagga gaacacaaat ggaaaagcaa acaatcacta tctatgatgt
    29401 agcgagagag gcaaatgttt cgatggctac tgtttcacgt gtcgtaaatg gcaatccaaa
    29461 cgtcaaacca gcaacaagaa aaaaagtctt ggaagttatc gatcgcttgg attatcgtcc
    29521 aaacgcagtt gcgcgtggct tggcaagtaa gaaaacaaca acagttggtg taatcatccc
    29581 tgatgtcagc aatatgttct ttgcttcttt agcacgtggt atcgatgatg tggcaacaat
    29641 gtacaaatac aatattatct tagcaaattc agatggcaac gaccaaaaag aagtcaatgt
    29701 attgaataac ttactggcta agcaagtgga cggcgtgatc ttcatgggtc accatatcac
    29761 agacgaaatc cgtggagaat tttcacgttc aaaaactcct gttgtattag ctggctctat
    29821 tgatccagat gaacaagtag gtagcgtaaa cattgattat gtatccgcaa caaaagatgc
    29881 agtaaacaaa ttggcaaaaa gcggcaataa aaaaatcgct tttgtcagtg gtgcgttgat
    29941 cgatcccatc aatggacaaa atcgtctgaa aggctataaa gaagcattaa gcgaaaataa
    30001 cctaccatat agcgaaggat tgatctttga agcacaatac aacttcaaag atggtctatc
    30061 tctagtcgat cggatccata atagcggtgc aacagctgtc tatgtttcag atgatgaatt
    30121 agccatcggt attttggatg gtttattaga ccgtggcgtg aaagtaccgg aagaattcga
    30181 aatcatcaca agtaacaatt cactactgac agaagtagct cgtcctcgtt taacaagtat
    30241 cacacaacca ttatacgatt taggggcggt atctatgcgc ctattgacga aaatgatgaa
    30301 taaagaagaa gtcgaagaaa aaacgattat cttgccttat ggaatcgaag aaaaaggatc
    30361 tacaaaataa taaaaggaca accattcaga ggttgtgtgc ctgccgttta actctgcgtt
    30421 acaaggagca atttcaaaaa acagcatctc atgtttgatg aatattgtga ctcgtgattc
    30481 ggagttggca ggagaacacc gtttatttgg gaaaaggggt tatgacaaaa ttttgttata
    30541 atcctttttt tgtgtcttaa aaaagagaga aagtggttga aattatattt atcgtatata
    30601 aaacaatccg tgtatagatg ctgtattttg ttcaccatca tcaaaaaaat aagggaatgt
    30661 gacaatacta ttgtcacatt cccttattac tgtgtattct attaaatcaa tcatctttct
    30721 catctgattc tttggttgct tgattcgctc ttgcataagc tgaagcggtg ttccagtaat
    30781 ctgtatagtt ttcatcggta ccgccaacgc caaacttaaa ggcgcttttc ttcggtccat
    30841 ttttcgccca tagcgattca actgtttttc ctggtgtttg gatggatttt ttattgacag
    30901 taacttttcc aggtgtttga ccagtaaatc cagatacttt tttcttcatc acatcagatg
    30961 ataattcgaa tttctcatca gtgccaaaaa tgtcaggttt tgcttggtaa gcttgattga
    31021 tcagattggc aagataatta gccgaacgta tccttgcttt gctgtccatt ccagtattgt
    31081 catcgtgccc tgcccagcta ctgatggtaa tagacggagt agagacaatt agccatgaat
    31141 ctcggtattc attggtagaa ccagtttttc cgacccaatc tgcttttcct agatttccgt
    31201 ttaagcttga gatggcatct ttgaatggtg tagtgatttt tgcattgatc acactgcgca
    31261 tcatgtcatt catgatcgat gccgttgctt ccgaatagat ccggacagag ttgctttcgt
    31321 gtttataaat tacatttcct gcgttatctg tgatggaatc gatgatatag ccttcttcat
    31381 agacgccgcc gtttgctaaa gcctgaaatc cgttggtctg ttgaagcgtc gtgacatcta
    31441 ctgtacctaa aggagcagat tcaacgcccc aattgtcatt tgccgggtag ttcatctttg
    31501 acaagtagtg ctcgtaagca tattgtttcg atccaccgga attcaacaca tcttgatata
    31561 agtgataagc cgggatattg ttggaccatt ctagtgactc acggacggtt tggaaggtgt
    31621 tcgatccttc gttagtagca ttgacgattt tctcgccagc attttctcct tcttgccaag
    31681 ttgttgcata atctgataca cgggattctg atccgataag ccccatgtcg attgcaggtc
    31741 cgtaaacaag aactggtttg attgatgagc cagcttgacg ttctgtatcg aaggcatggt
    31801 tattttgatt ctgactgtaa ttccgtccgc caacgaatcc gtagatgcgg ccagttctgt
    31861 tatccatcaa tacatttccg gtttctactt gactgccagc accatcatct aacatgtatc
    31921 cataattttc aacgccattt tgcaatgcag catagacatc tttatcgatt gtcgaatgga
    31981 ttgtatatcc tttattttgg atcgtttgct gtgccttttg ataataagcg tcactgatcg
    32041 atgaatcaga catatcagcg ttgtcttttt cagccaattg ttgtgcaatg attcttgttg
    32101 cttcattcat cacagtataa tacaagaatt ctctgtcagt ctgttctgcg atttgttgtg
    32161 gtagaaagtc ttttgtaagg tcataggctt ttgcttcttc gtattgttct ttcgtaattt
    32221 ggccttcacg atacatactg aataaaacaa aatcttttct ggcaaggccg gcagaaagat
    32281 cttcttttag cgcacctgtg ttcgtgtatg gactatacgt gatcgggctt tggggtaatc
    32341 cagcgatata agcagcttgc ggcagagtaa catcttttgc atctacacca aagattcctc
    32401 ttgcagcttc ctggactcca gcaatgtttt gtcccttatt attacgacca aatggtgaga
    32461 cattcaaata cgtagcaatg atctcatctt ttgaaaagta tttttctact tgtgcagaaa
    32521 gaatgatctc gtttgcttta cgtttgaatg ttgtttcatc tgtaaggatc tgctgtttga
    32581 ctaattgctg tgtcaaagtt gatccaccag aagagccaat accagtagct tcagatacta
    32641 atgctcgtaa aacagcttta ggaacgtaac cttggtgttt atcgaagtat tcatcctctg
    32701 tgctgataat tgcagttttc aataaaggtg aaattttatc agatgaaata gtcgtgcgca
    32761 tcagatctgt ctggattttt gatatctctg tattatctgc ataaacaatc tttgacgttt
    32821 ctgtaatgtt tcctagttct gtctgcagtg cttttttagt cggcaattgt gtgtcttcga
    32881 cgagataggc aaaatagcca gcgcctattc caatacctag agctgcgaac atcaaaagaa
    32941 gaacagtaat aaaaacagtc aatgattgaa aaactctaaa aataatctta ggaacaaacc
    33001 atttttcttt ttttcttcta ttctgttttt ttcgagtatt tttgcgtggc aaaagggtca
    33061 cctcaactaa tggttttatt tcgctaatta tagcaaataa tagaacaaaa aaacagacaa
    33121 ggctgttttt ggcaggaatt tcttttttaa aggagttaaa acgtgagaaa atgatctttt
    33181 ttataaaaga gttcttaagc ttttctttag agaaaatttc agttactatg gagaacaggg
    33241 caaagaaaaa tcaatcattt tctttgtata tcatcttacc aatatatagg aattgttagt
    33301 aacaattgcc tttcaaggag gaatccatcc atgttattag gctcacacgt cagcatgagc
    33361 ggaaagaaaa tgctgttagg agcagcagaa gaagcagcca gttatggatc ttcgactttt
    33421 atgatctata caggcgcccc tcaaaacact agaagaaaag ccatcgaaga gatgaatatt
    33481 gatgccggaa aagcgtacat ggcagaaaac ggtctaacaa atatcgtcgt tcatgcccct
    33541 tatatcatta atttggggaa tacgatcaaa ccagaaatgt ttccttttgc cgtccaattt
    33601 ctgagagaag agattgttcg tgcggaagct ttaggcgcta ctcaaattac acttcaccca
    33661 ggtgctcatg taggggcagg aacagcagcc ggaattgcta gaattattga aggcttgaat
    33721 gaagttttga caaaagaaca gacggcacaa atcgctttgg agacgatggc aggtaaaggt
    33781 acggaaatcg gtcgaacatt tgaggaatta gcgcaaatca tcgatggagt aaaattgaat
    33841 gaaaaattat ccgttacttt tgacacctgt catacgaatg atgccggtta taatattaaa
    33901 gaagactttt caggtgtaat ggaagagttt gataagatca tcgggttaga tcggcttaaa
    33961 gtactccata tcaatgattc taaaaatccg caaggttcgc ataaagaccg gcacgccaat
    34021 attggttttg gaacaatcgg attcgacacg ttgaataaga tcgtccatga tccaatgttt
    34081 gacaatattt cgaaaatctt ggaaactccc tatgtcggca aagataaaaa gaaagcttac
    34141 gcaccatatg gaaaagaaat cgccatgttc aaagcgcaaa cattcgatcc agaagtgttt
    34201 cctgagttat tagcagaaga agtgatactc taagattttc tatttgacag cagcaaaaaa
    34261 ggaccaggca cattgccttg tccttttttg tttaagaaac aaaggagtac tttttgcagt
    34321 ctttttcatc atgcatcttc tcccccctct ttttaaattt agacaaacag ggtatactgg
    34381 tagaaaagta aaaaaacgaa aagtttcaca agcaagtggc aaaacgtgat tggaaggacg
    34441 aaaagatgct aaacacttta aaacccttga tcaaccaact gactaagaaa catacatact
    34501 ttattcttgg catagcccta ttatttacaa ttattcgttt gtatttatat agtaaagcaa
    34561 tctatggaac agacatgtcg gcaacctatg atgatcagtt gctgattcgc tatgctcaaa
    34621 atattttgga cggtaaatgg ctaggggagt atacgactac aacactttcc aaaggaatct
    34681 cctacagctt atttttagta cttgcacata aactgcattt ttcttatgca gttttattcg
    34741 gcatgttcag tatttttgcc agtatattgg ttgcactcgc gtttaaaccg atcgtgaaga
    34801 acaagtatct tcttttaagt atctattttt attttttgtt ttctccgatt agttttacac
    34861 atgaatattc cactcgaacg tatcgtaatg ccctcgtgat tccggctgtt gagatcatta
    34921 tcgcttgttt attagctatt tattaccgca aatacggttc tagcagaaaa ttgctgccat
    34981 ggtttttagg gctttctgtc gcttttcctt ttttttggtt cattcgcgaa gattctatct
    35041 ggcttcttcc ttttactgta gtagctttgg gtatcagttt cgtccagatc ttgctagggc
    35101 aagacttttc tttcaaagaa gtaaagaagt taaaaaaacg actctttctt ctctcgaaac
    35161 gggaatggct gaaactttca ttgtttttac ttcctatagt tggcttttcc atcgtctatc
    35221 agtggatcga atataaaaat gagacaacat acggtattgc aaccatcaat gaccgttcga
    35281 gtggagaatt tggacgtttg atgaaacaac tgatccgaat agatgatgaa acgaacttga
    35341 ataaagaaaa cagtgagata tgggtctcac atgctgcact aaataaagca ttagccgcat
    35401 ctcctacatt ccatactgtt tctacccgta ttgacgaatt atatacgact cacgcgtgga
    35461 ctcaaggtgg aaaagtaaaa gaattacccg gagatatcat cttttgggca ttgcgagatg
    35521 tagtaagcga atctggttat taccaaaaca atgcaaaaga aaccaatgat ttttggaaaa
    35581 aagtcaacca agaattagaa gaagcctatc acaagggaac catcaaaaag aaaaaagagt
    35641 tctatctgat gggatcagga gatggaaaga cgatgagtga tctgccaatc gtattcgatt
    35701 tccttcaatc aggatacgct tatgcatggc gatataaaga tttccatcaa ggaggaaatt
    35761 atagtttcgg aacgcaagaa caagtcaaac aggctcaaga attattaaac cttccgctaa
    35821 tggagaattg gatcgatagt aatgaaccga gaacacctaa cttccaatta acaaaatcag
    35881 caaaagtcgc gaatgtcatc ataaaaattt accaagttct ggctattccc gtgctgattg
    35941 cttcaatagc agggatatta cttatgatac taggaaccgt atttgcaaaa gctgatcgtg
    36001 cctattatag ttcctctttg attgtggtca ccggattaat actgacacaa ttggtttttc
    36061 ttttcggtgt ctcatggttt tgttcttatg cgccagaaaa aaaggactat tttttaagtg
    36121 tctacactgg tgcaggcgtg ccattaacgc aattcatcgt agtaacaagc atgctagttt
    36181 tcagtaaatt aaatctgaaa aaagctttat cttctaaata aaaagaccct tggtagctag
    36241 atttttaacc taactatcaa gggtctttgt gtggaaaaat cagtttttat tcgatttctt
    36301 ctaccgtttc ctgcaaaagc tgttcatatt ttttgtttcc tttatataaa tcaccaatcg
    36361 tccatgccaa caggagaagg acaagaacaa tgattacata agcgatgtga tcagccaatg
    36421 ccagttgagc ggaagtggct tgctcgccga agaaactttc gatctgtcct ggtaatcctc
    36481 gtaagttcaa gtaagttaga ctaatcacag agaaccagcc aagaagtttt acgatgaatg
    36541 aatttttaaa tcggctaccc atctccgctt ggctgttggt catcatcagt aaaggaatca
    36601 tcgaaaatgg caacgcaaaa gccaagaaca cctgggattc attcattaag ttattcaaag
    36661 cggtatgttc ttcgatcgtt ccttttgtac ttgtaaacaa aacacaaatc aatacaggaa
    36721 tcacagaaag caatcgtgtg accagtcttc ttagccaaat cgggatacgc atatggatga
    36781 atccttccat gatgacttga ccggtcaacg taccagtaat agtagagttc tggccagaag
    36841 caagaagtgc tacagcaaat aaagtggaca agataccaga ttttgctaca gcaatcaaaa
    36901 taccattgct catagctgat ggatcagata aagcttcata cagaccaaaa aaggaaggat
    36961 cttggacaga accggttttg aagacagcga cacccatgat caatagcagt gcattgacta
    37021 gaaaagccat tgacaactgg atattagaat cccaagtaga aaaacgtaca gcttgtgcta
    37081 catcttcttc gttttggtga tcgatcttcc gagtttgtga aactgctgaa tgaagatata
    37141 agttatgcgg catgactgtt gcaccaataa tccctaaagc tccagttagc ggagtttcgc
    37201 caccaatgac aggtgagtct gagaatgtat ggctgttagg gataaagcca gcaaataaac
    37261 tgtgccaatc tgggttagac aagccaactt gatagacaaa aacgaataaa atgacgaaaa
    37321 ttaagcagac aaccagtgct tcgatcttac gaaacccgac ttttgtcagt agtaagagaa
    37381 cgaaaacatc taatacagta ataaagacgg caatcaccaa tggaatatca aaaagtaagt
    37441 agagggcgat tgctgcacca attacttctg caatatctgt tgccataatg gctaattcag
    37501 tcaagatcca taaaatgaca cctaatgtct tacttgtgcg tgctcggatt gcctgtgcca
    37561 aatccatttg gctcacgatt cctaattttg ctgccatata ctgtaaaagc atagcaataa
    37621 gactagacat caaaataact gacatcaata aatattggaa gttttgtcct ccggtgatcg
    37681 aagtagacca gttacccgga tccatatacc caactgctac taacgcacca ggtccagagt
    37741 aagcaagaag tgttttaaaa aaacttttcc cctttggtac atcaactgtt ccatttactt
    37801 cagctaaaga taaaccattt gtatgttcaa taagtcgatg cttatgaaat tgtttattgg
    37861 attgattatt caagaaaaaa ccacctttcc tatttatcta cgattacatt ctacacctca
    37921 aaaagaaaaa attaaagaaa aagattcggt atgcctaatt tattttcgtg gcgtgtcttg
    37981 ctagatgacg aaagaaattt tgttctcctc taaaaaaacc gctatttttt gcatgaaact
    38041 aaagaatacg cctttctttt ttcaataaac tttagtaaaa taagtcattg agagcaattc
    38101 ggaaatagaa aggagagatt tatatgttat caacaggtat tgtcgtactt attgctatta
    38161 ttgctttgct aatcggcgca gtaggtggct ttttcctagc gcgtaaatat atgaaagact
    38221 atttagaaaa aaatcctccc gttaatgagg acatgttgcg atcaatgatg atgtcaatgg
    38281 ggcaaaaacc atctgagaag aaaattcgcc agatgatgca gcaaatgaag aaccaaggga
    38341 aaaaataggt tctgacccaa cgacttttta cgggctgatt ttttcagccc gttttttatt
    38401 tcgtaatttg ttagactatt cagattattc agcaaaaaag acaagcacag acaatcctag
    38461 gctcttgata aagacaaagg aggagtaaaa tgtcgatttt tagaaaatta ggctggtttt
    38521 ttcgtcagga aaagaaacat tatttaattg gagttttctc gctattcgca gtggccttgg
    38581 tacagttagt tcctccaaaa gtgattggga tcatcattga tgagattgcc gataaaaatt
    38641 tatcaataaa gatcattttc gggtggatct tcctattgat tatcgctgct tttttacaat
    38701 atctttttcg ttatatctgg cggacgaata tttggggaag tgcagctcgg ttggagaaag
    38761 aacttcggca gcagttattc aaacatttta cagaaatgga caatctcttt taccaaaaat
    38821 atcggactgg ggatttgatg gctcatgcaa cgaatgattt gaatgcgatc cagaatgtag
    38881 caggagctgg tatcttgacc tttgcagatt cagtgattac gggcggaaca acgatcattg
    38941 cgatgatttt gtttattgat tggcgcttaa cactgattgc gatgattcct ctgcctttgc
    39001 tggccatcac ctcacgagtg ctgggatcga aactccatga tgcttttcgt gattcgcaag
    39061 aagctttttc tagtatcaat gataagacac aagaaagtat cactgggatc aaagtcatta
    39121 aaacatttgg acaagaaaaa gaagatctag cggattttga agaaaagatt gaagatgcaa
    39181 ttattaaaaa caaacgagtc aattttttag actccttatt tgatccattc attaccttga
    39241 ttatcggtct gtcttatgtt gcaacaatta ttttaggtgg aaagtatgtc ttggaaggca
    39301 cgatctcgat cgggcagatg gtctcattta ttagttatat cggtatgctt gtttggccga
    39361 tgtttgcgat tggtcgattg ttcaacgtat tggaaagagg aaatgcttct tatgatcgcg
    39421 tcaatgaact tttgcatgaa aaaacgcata tcgtggaaca actgggtgca gtgcagaccc
    39481 cggcaaaagg cgagctgaat gtagctattt cctcttttaa ttatctgaaa gaagaatcac
    39541 cagcattaga acaaatcaag tttgcaatcc aagaaggaga aacattaggt attgttggga
    39601 aaactggtgc gggaaaaacg acgatcatca aattactgat gcgtgaatac gatgattatc
    39661 aagggaaaat ccaatttggc ggacgttcga tcaaagaata ttcattgaat gctttattag
    39721 gatcaatcgg ctatgtgcct caggatcact tcttgttttc gatgaccatt cgagataata
    39781 tccgtttttc taatccaaaa ctttcagaag aagaagtaaa gcatgcggct tctttagcat
    39841 ttatcaacaa tgaaatcaag gaattaccaa aaggttatga taccttagtt ggagaacgtg
    39901 gagtctcttt atctggcgga cagaaacaaa ggatttcgat tgctcgagca ttgatcaaaa
    39961 atcctgaatt attaatgtta gatgatgcac tttctgcagt agatgccaaa acagaagaag
    40021 ctatcttgca taatttaaaa gaaataagaa aagagaaaac aactatcatt gcagctcacc
//
LOCUS       DACDPS010000017        39076 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.017, whole genome shotgun sequence.
ACCESSION   DACDPS010000017 DACDPS010000000
VERSION     DACDPS010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39076
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.017"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(159..2540)
                     /locus_tag="IWJ07_000952"
     CDS             complement(159..2540)
                     /locus_tag="IWJ07_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP9332549.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2530..3177)
                     /gene="recU"
                     /locus_tag="IWJ07_000953"
     CDS             complement(2530..3177)
                     /gene="recU"
                     /locus_tag="IWJ07_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAP9332550.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3241..3780
                     /locus_tag="IWJ07_000954"
     CDS             3241..3780
                     /locus_tag="IWJ07_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAP9332551.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3870..4289
                     /gene="gpsB"
                     /locus_tag="IWJ07_000955"
     CDS             3870..4289
                     /gene="gpsB"
                     /locus_tag="IWJ07_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAP9332552.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4311..4678
                     /gene="rnpB"
                     /locus_tag="IWJ07_000956"
     ncRNA           4311..4678
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IWJ07_000956"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4887..6053
                     /locus_tag="IWJ07_000957"
     CDS             4887..6053
                     /locus_tag="IWJ07_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAP9332553.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6099..7595
                     /locus_tag="IWJ07_000958"
     CDS             6099..7595
                     /locus_tag="IWJ07_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAP9332554.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7676..8359
                     /locus_tag="IWJ07_000959"
     CDS             7676..8359
                     /locus_tag="IWJ07_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9332555.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            9004..10194
                     /locus_tag="IWJ07_000960"
     CDS             9004..10194
                     /locus_tag="IWJ07_000960"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAP9332556.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10296..11249
                     /locus_tag="IWJ07_000961"
     CDS             10296..11249
                     /locus_tag="IWJ07_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAP9332557.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11318..12667
                     /locus_tag="IWJ07_000962"
     CDS             11318..12667
                     /locus_tag="IWJ07_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9332558.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(12850..13173)
                     /locus_tag="IWJ07_000963"
     CDS             complement(12850..13173)
                     /locus_tag="IWJ07_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332559.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13302..14465
                     /locus_tag="IWJ07_000964"
     CDS             13302..14465
                     /locus_tag="IWJ07_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332560.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14535..15884
                     /locus_tag="IWJ07_000965"
     CDS             14535..15884
                     /locus_tag="IWJ07_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP9332561.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(15929..16360)
                     /locus_tag="IWJ07_000966"
     CDS             complement(15929..16360)
                     /locus_tag="IWJ07_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9332562.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            16748..19390
                     /gene="alaS"
                     /locus_tag="IWJ07_000967"
     CDS             16748..19390
                     /gene="alaS"
                     /locus_tag="IWJ07_000967"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAP9332563.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19590..20186
                     /locus_tag="IWJ07_000968"
     CDS             19590..20186
                     /locus_tag="IWJ07_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9332564.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20308..21456
                     /locus_tag="IWJ07_000969"
     CDS             20308..21456
                     /locus_tag="IWJ07_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP9332565.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(21494..21907)
                     /locus_tag="IWJ07_000970"
     CDS             complement(21494..21907)
                     /locus_tag="IWJ07_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAP9332566.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            21986..22417
                     /locus_tag="IWJ07_000971"
     CDS             21986..22417
                     /locus_tag="IWJ07_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAP9332567.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(22516..22749)
                     /locus_tag="IWJ07_000972"
     CDS             complement(22516..22749)
                     /locus_tag="IWJ07_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAP9332568.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            22932..24602
                     /locus_tag="IWJ07_000973"
     CDS             22932..24602
                     /locus_tag="IWJ07_000973"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAP9332569.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            24749..25135
                     /locus_tag="IWJ07_000974"
     CDS             24749..25135
                     /locus_tag="IWJ07_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332570.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            25122..25820
                     /locus_tag="IWJ07_000975"
     CDS             25122..25820
                     /locus_tag="IWJ07_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9332571.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            25825..26289
                     /locus_tag="IWJ07_000976"
     CDS             25825..26289
                     /locus_tag="IWJ07_000976"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAP9332572.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            26286..27197
                     /locus_tag="IWJ07_000977"
     CDS             26286..27197
                     /locus_tag="IWJ07_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP9332573.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            27432..27971
                     /gene="pyrR"
                     /locus_tag="IWJ07_000978"
     CDS             27432..27971
                     /gene="pyrR"
                     /locus_tag="IWJ07_000978"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAP9332574.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            27985..29268
                     /locus_tag="IWJ07_000979"
     CDS             27985..29268
                     /locus_tag="IWJ07_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAP9332575.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            29281..30207
                     /locus_tag="IWJ07_000980"
     CDS             29281..30207
                     /locus_tag="IWJ07_000980"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAP9332576.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            30226..31506
                     /locus_tag="IWJ07_000981"
     CDS             30226..31506
                     /locus_tag="IWJ07_000981"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAP9332577.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            31511..32587
                     /gene="carA"
                     /locus_tag="IWJ07_000982"
     CDS             31511..32587
                     /gene="carA"
                     /locus_tag="IWJ07_000982"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAP9332578.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            32592..35777
                     /gene="carB"
                     /locus_tag="IWJ07_000983"
     CDS             32592..35777
                     /gene="carB"
                     /locus_tag="IWJ07_000983"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAP9332579.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            35831..36607
                     /locus_tag="IWJ07_000984"
     CDS             35831..36607
                     /locus_tag="IWJ07_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAP9332580.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            36604..37536
                     /locus_tag="IWJ07_000985"
     CDS             36604..37536
                     /locus_tag="IWJ07_000985"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAP9332581.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            37533..38249
                     /gene="pyrF"
                     /locus_tag="IWJ07_000986"
     CDS             37533..38249
                     /gene="pyrF"
                     /locus_tag="IWJ07_000986"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAP9332582.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            38256..38885
                     /locus_tag="IWJ07_000987"
     CDS             38256..38885
                     /locus_tag="IWJ07_000987"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAP9332583.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            38873..>39076
                     /locus_tag="IWJ07_000988"
     CDS             38873..>39076
                     /locus_tag="IWJ07_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332584.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRII"
BASE COUNT        12573 a         6916 c         8515 g        11072 t
ORIGIN      
        1 tttctaattt ttaagctaaa tgttagatca cacatgtata tgctggatag tcttataaaa
       61 aatcatgtaa tacagcttgg tacatgattt ttatttgtag ctgtttttta catagacaca
      121 aaaaagaaac cacgcttgta gacgtggttt cttttttact atctgtttcc agaagctgag
      181 gtgctgctgg atggtgtatg gtcatttgca gcatccgctc cgctagttgc tggctcactg
      241 gaagaagcag gaggagcaga tgaagacggt gtcgagcttt cttcggatga agaagaagga
      301 accgttgttg aagacgctgg ctcgcttgtt tctgctgtcg tgctttcttt tgaggactca
      361 gcagttgatt cgctttgact agtagagcta gagctttgag tcgttgtctc tgttgttgag
      421 cttccaggag tctggacata gctggaagat ggaatagttg ttgatggtgt aattgcattc
      481 gatctagctg tatattgatc tttgtagtac agttcaccgc ccacacgaat taatccgctt
      541 ggcatctccc agtctgtatt tgtcacgtta gctgaaacat actgcataag ctcgcgataa
      601 acatcagagg ctacgtgtga cgattctgaa gtgacaggtg tcattttttt attgtaacct
      661 gtccacacgg aaatcgaata atttggcgta tagcccgcaa ataggatatc tggatacacc
      721 ccagaagaaa taccaagttt tgcatactcg tcatctgtgt aattggatgt tccggtctta
      781 ccggcttgat ataacccagc gatttgcgca ttcgtaccag ttccttcagt gatcgtatct
      841 tttaaaatgt ctgtgatcat ataagctgtt tctggagaca tggctgtttt tccatctggc
      901 tcataagttt cttctgttcc gtcttggaag acgattttat taacatattg aggtttgtaa
      961 taagtccctc cattggcgaa agccgcatag gctgctgcca tttttaagga agaagctccg
     1021 tattttgtcc catcttgttc ttccgtatta ctagagatcg cattcgattg atggatcgta
     1081 ctgtattcga tcccaagatt ttttaagaaa gaagccactt tatctgaacc gacttcattg
     1141 aataatttga ctgctggaac attacgggat aagtaaagtg cttggcgtaa agtaatggtg
     1201 cccatatatt gattatccca gtttcctaca ggagtcgaag tcccttcata gttatacggt
     1261 gcgtcagtaa tagtttttcc agtagaatat ttcaaatatt caaaagctgg tccgtaatct
     1321 gtgacaggtt tcatcgtaga accaaaatca cgggaagtat tcactgctaa gttgttgcct
     1381 aaagttacgt cttctgcgat gtgacgtccg ccaatctggg cttttacttt cccggtattg
     1441 gtgtcgataa gggtggaggc aacttgcatc tcgtcgtctg ggtaagagac atactgatcc
     1501 gtgttgacga tatcatatag tttcttttgg gcgtccagat ccaaattcgt ataaatctct
     1561 aaaccgtctg tgtagacatt cttatctgtt ttttcttgta cttcattgat gacttctttg
     1621 acataattat cgacgatctt agtattgtca tcggattgag ttagttcttg cagaccatcc
     1681 gttaccggta cattaacagc ttgatcatat tctgtttgag agattttttc gttttgaagc
     1741 atcgtataca aaacagtatc tcgtcttttc ttcgcctgat ctggataaac ataaggatca
     1801 taggcggaag gggcttgagg catcccagca agcaatgctg tttgcggtaa agaaagttct
     1861 gataatttct taccaaagta catctcagaa gctgtctcca tcccgtacaa gccgtttgac
     1921 atatacactt tatttacgta gtaggtcaag atctcttgtt tcgatttttt ttgttccaat
     1981 cttacagcca tccacgcttc ttgggccttg cgtttcagag tctgatcctc ggcactggtg
     2041 gagaagaagg aaagtttgat caattgctgt gtcaaagtac ttccgccttg cagtccgcca
     2101 gaggtaaagt tagagagggc agaaccgatg atccgaatag ggtcaacccc gatgtgttta
     2161 taaaaacggc gatcttctac cgacacgatc gcatcttcta atgttttcgg taattcattc
     2221 gcagaaattt tttcacgctt ttctgcgcct aaatcttcga atagttcgcc atcttgtgta
     2281 tatagtttgg aagatacggt agcgtctaat tttttatccg tcaattcggg cgcatctttt
     2341 gcgtaatacc agaaaagtcc tacacctgct aaaaagagga tacagaaaaa tgagagtatt
     2401 cctaaaagga tcttgataaa taatcctttt ttatgtgttc cagaggactt tccagaacct
     2461 ttgccagaat tcttcttcac actcttttta ggagtaggct gtttctgtcg tctagagctt
     2521 ctagtttgtt catttgccat tcttgctttc tccttcaata gataatgttg ataaatagaa
     2581 gtcaaccgcg tccaaataag gaatccgcgg agaaatacca gttttgatct ctattcctag
     2641 ttcttcgatc aaagataaag gtagtgattt gcgtcctttt tccttttggg cgtcccagta
     2701 agcaatcaaa tgaacactat tcaaataaaa acagcgcttc aaagacgaaa accataacaa
     2761 gacaaagcag accccatttt gttcgaggca tgctttcata tgttcgattt gatgggcatg
     2821 gaagttttta aagggaaaag atgttttgtt cttggtttct tttgcttcaa agtccaagta
     2881 aaagcctcga tagacgccat tatagtctgt agttgaggct tgggtaaagt aggcttcctt
     2941 gatcacagca gcacttctct taggatagtc tactttcacg atttggatag gggtaggctt
     3001 tttatggata accgccaacc cgcgggaaag ataatattta ttactttcat tgattgcttc
     3061 ttcaaagcgc atcccccgat tgccgaattc agtgatctga tttctttttt cttgaatttt
     3121 ttcgacggga gaggcattct tataataagg ttggccattt ggatagcgca ataccatctt
     3181 taatcacact cctaggccac attatagcaa aaaagcggat agaaagaaaa ggaatctgag
     3241 atggataagt caaaagtaat ctatgtgtct gggtatcgaa gtttcgaact cggtgttttt
     3301 aaagaaaatg atcctaaaat ccaagttgtt aaaaatgttt taaaaaaaga actccatcag
     3361 ttagcagaag aagggctgga atgggtactt gtcagtggga atttgggcgt cgaactatgg
     3421 gctgtgcaaa tagtagcgga attgaaagaa gaatatccgg aactatctct tggtattttg
     3481 tatccttttt ctgattttgg taataactgg aatgaaggga accaagaaaa aaaacaatta
     3541 gcagaacaat tagcagatta tgtcgaagct gttagccatc aaccctacca atcacctagt
     3601 cagctaaaga atcacactcg ttttatactt gagcatacag tgggctgtct gctgatctat
     3661 gatgaagaat tccctggaaa aacgaagtat ttttttgaag atgcacagaa ttaccaagca
     3721 gatcatccct acgaaattcg gctgatcagt atggacgatc ttcaaaattt tagtgaataa
     3781 tcgatattgt gtttgattta tcaacttttt tcctatacaa tgaaaaaggt agaaatgtta
     3841 ttacgtaaat gaaaactaga ggtgtaaata tggcgaattt ggtttatagt cctaaagaca
     3901 ttttacaaca agaattcaaa acaaaaatgc gcggctatga tcccgttgaa gttgatgaat
     3961 tcttagacaa tatcatcaaa gattatgaaa cttacagcaa agaactcctt gctttacaag
     4021 aagaaaacga tcgattgagt gcaaaagtag ctcaattatc taaaactcaa ggagcagctc
     4081 aaacacgcgt gcaacaaaca gaggcaccaa agagcgcagc agtgaccaat tttgatatct
     4141 taaaacgctt gtcaaatctt gaacgtgaag tatttggtaa aaagcttgat caacaagcaa
     4201 gtgcagtcaa accagcacaa cctaatccaa ataactacac aaatgcagac acaagcttag
     4261 atgataatga aaaaacacgt caattttaat ttttgattaa tgaatttgcg gttttcgggt
     4321 aatcgcggtc tgacttgatc aggctgagga aagtccatgc tcgcacaggc tgtgatgcct
     4381 gtagtgttcg tgcttagcga aaccataagc taaggtactc tttggagtaa cggcaggaaa
     4441 acaagctaag gcttcggcta tgcttaagta tccttgaaag tgccacagtg acgaagcggc
     4501 tggggaaacc catccggtgg aacgcggtaa acccctcgag cgagcaaccc aaacaatggt
     4561 aggggcgctt tttgtctgga agaagaacag gacaaaaagg cagtataact gcagatagat
     4621 ggttaccgct ttgttttttc tccctggaaa agcaaagtac agaacatggc ttatagaaaa
     4681 ccgcaaacat cagggaatta ggtcgtgaca aaagtaatgc gttataagaa acaagaaaaa
     4741 catttgaaag ctatttttca atacttcaga tgaatcgttc ttattacaaa acaaactttt
     4801 gtatcaccgg ttattcggaa tttgagtaaa agtgtggggt aggactgatg tcccacctcc
     4861 tttttttata aataaggaga ttgtgtatgg aaaaaaacag gaagttcaac ctcgtcgcaa
     4921 ctgcggcaag cgggctagaa gcactggttg gtaaagaatt aagagatctt ggaatcgaat
     4981 gccaagttga gaacggacgt gcacgttttg aaggaacaat ggaaacgatc gctacagcta
     5041 atttatggtt acgtacagca gatcgagtaa aaatcgtggt tggggaattc gatgcgtata
     5101 cgtttgatga attgtttgaa aacgtaaaag cattgccttg ggaagatttt cttccactag
     5161 atgcagcttt tcctgtagct ggaaaatcga tcaagtcgaa actatatagc acacctgatt
     5221 gccaagcgat cacaaaaaaa gcaatcgttg aacgtctccg tacctattat caccgtccag
     5281 cttctgtacc actgacggaa actggcgcac attttcagtt ggaagtagct ttgctgaaag
     5341 accatgtgat ggtaactctg gatacgacag gaccaagttt attcaaacgt ggttatcgct
     5401 tagaaaaagg tggagcacca ttgaaagaaa acatggcagc agcacttatt atgttgacaa
     5461 attggcgaaa agaccgtcca ttttatgatc ctgtatgtgg ttcaggtacg ctttgtattg
     5521 aagcagcctt aataggccat aatatcgcac caggattcaa tcgtgagttt gcttgcgaat
     5581 cttgggattg gttcagtcaa gaaatcatgg aaaatgttcg atcagcagcc gaagaaaaag
     5641 cagattatga tattgaatta gatattactg gttcagatat caatggacgg atgattgaaa
     5701 ttgccaaagc caatgcagaa gaaatcggat taggctcttc gattaccttt aagcaacaag
     5761 cagtcaaaga cttcaaaacg gataaagaat acggggtcat cgtggctaat ccgccttacg
     5821 gagaacgtct aggagaagaa gaaactgttc ggaaattata taaagaaatg ggcgatgtat
     5881 tccgtccatt aaaaacatgg agtaaatata tcttgactag cgacttagcg tttgaggaat
     5941 tttacggaca aaaagcaacc aaaaaaagaa aattgtacaa tggtgcattg cgtacggatc
     6001 tattccagta ttggggaact cgtccgccta gaaaaaaaga gaaagacgaa taatcttact
     6061 aattgattca ttcaattgga tgtttggagg aatagggaat gaaagaaaaa gagtttttac
     6121 aagaagtaaa agaaatcaat cttctacaac aagctttagg aatcctcgat tgggatacac
     6181 aaacaggtat gcctgaaaaa gctagtgcat accgaagtga agtagacagc tatctttaca
     6241 gcatttattt ttcaaaaaaa atcggtccaa aaatcaaaga agccattcgc tatttttctg
     6301 aacatccgga agaattgtcg gaagttggaa ctgccgtttt tgccaaagta aaagaagagt
     6361 atgatttgga acatcgcgta ccggaagatc ttatgagaaa atatacagct gccgtttctt
     6421 cagcacacgg acaatggcaa aaagctagag aaacacaaca attttctgat tttcaacacg
     6481 cattgaatca aaatattgaa ttaacaaaac agttgatacc ttactggaga aaagatgaaa
     6541 agacgaatta tgatgttttg ctgaatcaat acgaacccgg gatgactgtc gaaatccttg
     6601 ataaggtatt tgatcagctt aaagaaggaa ttctttcgat ccgccgtacg ctccaagaaa
     6661 aagggactga acctgaaaca gactttttaa atcggagaat gacgaaaaaa caacagaaga
     6721 gatttgtagt gaaagtaatc gaacaacttg gttatgattt tagtcgtggt cgtttagatg
     6781 acactgtcca cccatttatg actggaatta atccgaatga tgcacgaatc acgacacgat
     6841 ggaacgaaac agatttcaaa atggctgttt ttggagtgat ccacgaggct ggtcatggta
     6901 tgtatgaaca agacatcgat gataaatatg cgtatacacc ggtttatgaa ggtacatcaa
     6961 tgggtatcca tgaatcacaa tcgttgttta acgagatcat tattggaagt agtcgcagct
     7021 tctggaaaaa gcaatatcct ttctttcaag aatgtgcaga aggaacattc gatgatattc
     7081 cttttgacaa attttatcgt tcattgaacc atacaaaatc aagtttggtt cgtatcgagg
     7141 cagacagttt gacttaccct ctccatatca tcattcgtta tgaaatcgaa aaaatgattt
     7201 tcaatgaagg ggtagatgtt gctgaactac ctgaaatatg gaatcaaaaa tatgaagagt
     7261 atctgggaat acgaccaaca aatgatgtgg aaggcatcct acaagacgtt cattggtcag
     7321 gtgcaagctt tggttatttt ccctcttatg cattagggta catgtatgcg gcacaattac
     7381 ttcattcttt gcagcaagaa atagatgtgg aaaatgttct agcgtcagac gattattctc
     7441 caatcaaaaa ctggatgtca gagcatatcc atcaatatgg tgcatctaga aaaccaaatc
     7501 aattaatcat ggatgcaaca catgaaccgc ttaatccgca atacctgatc gactatatga
     7561 aagacctgta ttttaatgta tatcaggtaa aataaaatac aatttacaaa gatagctgaa
     7621 attcatctaa tctatagatg ttttcagcta tttacttaag aaagaagtgg aacgaatgga
     7681 taaattacaa gtgatcgctg attatagtat ggaccaatta gcgcatgatc aaacaggaca
     7741 tgggagcgat cataccaaaa gagttgtcaa gttagctgaa aaaattttgg agacggaacc
     7801 gcaggcagac cgctttgtga cgcttgcagc agcttaccta catgatacga ttgatgataa
     7861 agtcgttaaa aatgagaacg aggcaaagca acagcttcgt gtattccttc gctctttgcc
     7921 aatagtagag gaacagatct cgatgatttt ttccattatc gaaaatatgt ccttttctaa
     7981 aaatctctct gaagcggccg aactatctct ggaaggtaag atcgttcaag acgctgatcg
     8041 tatcgaagca ttaggagcaa tcggcatttt gcgtactgct tatttcggcg gtggacatgg
     8101 gcacccgatt tttgatcctg aactgtatcc gcaaacattc aaggacaaag aaaattaccg
     8161 aaaaggcacg acagtgatca atcattttta tgaaaaactg ttcttgctgt ctgataaaat
     8221 gaatactgat tacggatatg aagaagccaa acgaagagaa accttcatgc gtgaattctt
     8281 agaagaattt tttctggaat ggtctattga cgatgaaaat tttactccag acaattggat
     8341 caaaaataac aaaaaatgat ggaggttgtg acagaagtgg tcagctccaa gatatttcgg
     8401 atcattttcc gtaaaagtta cttctggaac actgtttacc aagaggtcgt gagcctgccg
     8461 ttcagctccg agtcacaagg aacaatttca aaaaatagct tctcgtgttt gatgaatatt
     8521 gtaacttgtg attcggaatt ggcaggagaa caccgtttat tcggaagaat aggactgtga
     8581 caagactttt gtcacagtcc ttttttcgaa ttcaacaagt aaaactttta agaaatattc
     8641 agagagtaaa attaaaaatt gctgataaac gaagatggaa attttatgta tttttattgg
     8701 tctatgcaat aaataaatta aatattcaga atacaattga ttttaacgaa agggcttcta
     8761 tcaatggttg tgttgtattt atctgcccag gtagagtaaa atagatcatt atctaaaagg
     8821 taattgttca tactatataa gtcatttgaa agtaaaaaga tactgtaaat aaaaaatgtg
     8881 gttaaataat tctcataatc agtgtaagcg ttatcaaaaa tatagcatac ttgctttgta
     8941 gatgaatgac acgatatatg tttggtatgt tctgcaagac agatgaatat ggaggtggag
     9001 acaatgtctt ccaaagaact aacaatgtat ttagagaaag aattggatca actgaaagaa
     9061 aaggggttat acaatacgat cgacatacta gaaagtgaaa atggtgcttg tatcatcgta
     9121 gacggaaaaa agatgatcaa tctagcatcc aacaattatc ttggatttgc caatcgcgaa
     9181 gaattgaaaa aagcatgtat tgaagcaacc gaaacttatg gtgtaggagc gggagcagtc
     9241 cgaacgatca atggttcgct gaaaatccat cagcaattag aagaaaaaat cgcagaattc
     9301 aaagggacag aagcagcaat cgcctttcaa tctggattca actgcaatat gggcgctatc
     9361 tctgcagtaa tgacaaaaga agatgctatc ttatcggatg aattaaacca tgcatctatt
     9421 attgacggtt gccgcttatc cggagcaaaa atcatccgaa ttaaacatca agatatgaaa
     9481 gaccttgaga aaaaagcaaa ggaagcgata gaaagtaaga aatataaaaa aatcatgtat
     9541 atcacagatg gtgttttctc catggatgga gacattgcac gattgccgga aattattccg
     9601 attgttgaaa aatatggact gatcacctac gtcgatgatg cgcacggctc aggcgtaact
     9661 ggaaaaggtg caggaaccgt caagcatttt ggattaagcg acaaaatcga tatgcaaatg
     9721 ggcacgttat cgaaagctat aggtgttgtt gggggttatg tagctggatc gaaaacacta
     9781 atcgactggt tgaaagctag aagtcggcca ttcctatttt ctacttcgct aactccaggt
     9841 gcagctgcag ctgcattggc ttcgattact ttgatgcaag aacatccgga acttgtcgaa
     9901 aaagtttggg aaaatgctaa ttattttaaa gaagaattga aaaaagtagg ctataacatc
     9961 ggtatgtctg aaacacctat tactccagtc attttgggag acgaaaaagt gactcagact
    10021 ttctctaaga agctgatcga acatgggatt tatgctaaac caatcgttta tccaactgtg
    10081 cctcttggga caggcaggat cagaaatatg ccgactgcgg aacatacgag ggaaatgctt
    10141 gatgaagcag tagcagttta tcaaaaaata ggaaaagaaa tggaaattat ttaactaaat
    10201 ttagtttttt cgtctattta aaattagaga acatgttcac gcatatattg ttaatcatct
    10261 gaagatagta gtaaaaaaga tcagggagga tcatcatgaa aaaagtattg gtgacaggat
    10321 gtttaggaca aattggttca gagttgatca cacggctacg tcaagaacta ggcagtgaaa
    10381 atgtcattgc gacagatata cgcatgcctg aagtcattga gaaaaacgat atctttgaga
    10441 tcctagatgt aatggattac gaaaaaatgc gcagcatagc agaacaatat caagtggaca
    10501 cattgattca tttggctgct cttttgtcag ctgttgcaga agcaaaacca caattagcct
    10561 ggaatttgaa cgtgactggg ctggtcaatg cattggaagt cgctagagaa ttccagctga
    10621 aattttttac gccaagttca atcggcgcgt ttggtccaaa tactccaaaa gatcataccc
    10681 cacaagatac tcttcagcgt cctacaacca tgtatggggt aacgaaggta gctggagaac
    10741 tactatgtga ttattattat gtgaaatatg gcgtagatac tcgtggtgtc cgtttccctg
    10801 gattgatttc ttatgcaacc ttgccaggag gcggtacgac agactacgca gtcgatattt
    10861 attattcagc aatcaaagaa ggaaagtaca ctagtttcat tgatcaaggt acatcaatgg
    10921 atatgatgta tatgccagat gcgatcgatg ccattataaa actcatgaat gcagatcctg
    10981 atcgattggt tcatcgaaat gccttcaata tatcggcgat gtcttttgac ccggaagaga
    11041 tcaaagcatc tattcaaaaa atcataccag attttgaaat ggattatgat gtggatcctg
    11101 tacgtcagtc aatagcagaa tcttggccaa attcactaga tgtttcttgt gcgcaagagg
    11161 aatggggctt ttcgccgaaa tacgacttag atgtgatgac aaaggatatg ttggaaaaac
    11221 tgaaagaaaa attggtagag acggtataaa acaagaacca catccaaaaa gttttttata
    11281 cttttgggat tggctatggt atgaaagggg acaagggatg gaagaatatc aagccacgcc
    11341 agtaaaagaa gtaaaaatag aaaatgaatt aaaacgtacg atgggatttt ttactgcttt
    11401 atcgactgtt atgggcacag tgatcggtgc aggggtattt tttaaagccg caagcgtagc
    11461 agaagttaca ggatccgcca gtttgcatat gttttcttgg tttctcggtg gtatgatttc
    11521 tgtctgtgca ggactaacag gagctgaact ggcagctgct attccagaaa caggagggat
    11581 gattaaatat atcgaacgga tttacggaaa tacagcagct tttttattgg gctgggcgca
    11641 agtcgtgatt tattttccag caaatgtagc agccttgtcg atcattttcg gtacacagtt
    11701 cgtcaatcta tttggtcttt cccagtcaat gattgttccc gtagctgtta cagctgctgt
    11761 atcgatcctg ttgatcaatt ttttaggttc taaagctgga ggggcttttc agtcgattac
    11821 gcttgtctgc aagcttattc ctttgtttgt gatcgtcatt ttcggtttgt tccgacaaga
    11881 aggagtagac ttccaactgt ttccgatcca agcaggagaa aatctttcat ttttctcagc
    11941 cttgggagcg ggcttgctag caacgatgtt cgcatacgac ggttggatac acgtaggaaa
    12001 tatttcggga gaattgaaaa agccggcgaa agatttgcca aaagcaattt ctttaggaat
    12061 catcggaatc atgatcgttt atttattagt aaatgcggtg ttcttaagaa ccgcatctat
    12121 cgacggagtc gcaggaaaca gcaatgcggc aagtgacgtt gcaaaaatga tttttggcgg
    12181 cttcggtgga agactggtca cagttggtat cttgatttct gtttatggaa ccatcaatgg
    12241 ctataccctt actggaatgc gtctccctta tgttatggca aaagaaaaca acctgccttt
    12301 tagcaagctc tttgctaaac ttcatgataa aactaaagtt cctgtagccg caggtatctt
    12361 agagctagtc atcgctattg gcatgatgat gatcggtggt tttgatacac taacggatat
    12421 gctgatcttt gtcatttgga ttttttatac gatggtcttt gttggtgtca ttcttcttcg
    12481 taaaaaagaa cctgatttgt ttagaccata caaagttcca atgtatccgt ttattccact
    12541 agtagcaatc attggaggga cttttatcgt atcaagcacg cttatcactc agaccttttt
    12601 agcttcgatg gggatagtat tgacacttgc tggtatcccg atttatctct atttaaaacg
    12661 tagatagcta aaacattcga attttattag aactgcactt ctctgccaaa caaaatattt
    12721 tatttaaatg aggagtacgt ggaagaaatg tcaaaagtac tcgttaattt tgatcacgta
    12781 aatgtcgttt tgtctacgat gaaatagcaa agtaaaagac agaaatcggg ctcaaccgat
    12841 ttctgtcttt tactttgatt tgcgtaactg aactattcgg cgtacgccgc gataaatatt
    12901 taataaaacc aagcaaccag aaatgaaaat gggaagccaa atcagataga cagggtagcc
    12961 aagcacttta gacaaagcat gtggagtcat tttatccatt tcaagaagtg cttgctgcca
    13021 agtctgaaga ttgaagacta gcatgaaacc cgaaataatc aggagaataa atcctaaata
    13081 atgaattgta gttctttttc ttacataagc gatcgtagca taaaggaaca gtaaaaaaat
    13141 aggtacgcca aaaacaaagt acatattcag catctcgaca ccccctttgt gtttatctta
    13201 ctttactttg aaagaaatga gaaataatag cctcgttact tgcttgaaac agcaaaggac
    13261 tgctaagata agagagatac aaagaatcaa ggaggagtca tatggcaagt tatatcgaac
    13321 cgattcgttt tgcgctgatt gtttttccat ttttagcctt agcgatctca tctgtttttt
    13381 ttatttatga atatcgaaag tatggaacat ttctagtaac aagggcagtt attttgtatt
    13441 ctttcgtttt ttatttattg tgtgcgtatt ttttggtgat cttaccactt ccggcaaagg
    13501 agacggttgc ccaaatgaca gggccaaaga tggagctgca tttatttgct tcatggcaac
    13561 atttcagaga caacacggta ttggtcttgt ctgaacctag tacctacctg ccggcaatga
    13621 agcagagtgt atttttggaa ccagctttta atgttttatt actcatacca tttggcattt
    13681 atttaagata ttatttccgg ctttctttta tgaaaacgat gattgctagt ttctgccttt
    13741 ctctattttt tgaattgacg cagctaagtg gactttattt tatctaccca agaccatacc
    13801 gcttatttga tgtgaatgat ttagtgacaa atacattggg tggaatcatt ggttttgtcg
    13861 ttacgccgtt atttacattt atgcttccta caagaagacg aatggatgaa ctttcttatg
    13921 aaaaaggaaa gactgtctca ttgactcgac gcttggtcgc ttatttgatc gactggacat
    13981 ttttatctct tattaatttg gtagcggcga ttttctttaa aattgcgttc gattcagggg
    14041 cagttacttc tcatatctgg tggcttttat tagaagtagg ggtatacttt atgattgtac
    14101 cgtttataac caatggatgg acacttggca aaaaaattgt gcgtatccgt ttggtggaag
    14161 aagaccgcga acgaatttct ttaaaagcat tgatgatccg ctacgggtat ctgtactttc
    14221 ttttttatgg tttaagctgg ttggggcagt tgcttcagca aatcatgaat tcgacaacga
    14281 atacggtgtt gcagatgagt gcagtattca tttctctcgg tgtcttgtta ctacaaggta
    14341 tttttatttt aaatatcttg ctatcatttt tgagaaaaaa acggattctg ttttatgaaa
    14401 aagctagtca cacatatact gttagcacga ttggccaaac aaaggatgat gattctcgaa
    14461 attagctgag caaaaacgct ttgtctgtgt tataatgcca aagtcgtgat aatttaaaga
    14521 gaagaggtgc acgaatgaat aattttgata tattccgctt caaaccgttt atcaatgaag
    14581 cgttaaccgc aaagggcttc aaacagccca cagaggtcca ggaacgtttg attcctttaa
    14641 ttgcaaaagg aaaaaatatc attgggcaat ctcaaactgg tacaggaaaa acacatacgt
    14701 ttttgttgcc tttgtttgaa aaaatcaatg cagaaaaaga ggaagtacaa gttgttatta
    14761 cagctccaag tagggaacta gctgcacaga tctatgaagc agctacacaa atcgctaaat
    14821 tttctacacc ggagatccgt gtctcgaatt ttgtcggcgg tacagacaaa cagcgtcaat
    14881 taaatcgtct gcatcaccaa cagccacagg ttgtagtagg cacgcctggt agaatattag
    14941 acatgatgaa tgaacaagcg ctgaagattc acaccgcatt tgcatttgtt gtcgatgaag
    15001 cggatatgac attggatatg ggttttttaa atgaagtaga ccaaattgca agtcgattac
    15061 cagaaaagct gcagttttta gttttttctg ccactattcc agaaaaactt cgtccattct
    15121 tacgaaaata catggaaaat ccaatcattg aagaaatcaa accaaaagca gttatttctg
    15181 aagacattga aaattggctg atttcaacaa aaggtaaaga tgagaatcaa atgatttatc
    15241 aactgctgac aattggtcat ccttatttag caatcgtatt tgccaataca aaacagcatg
    15301 tggatgaaat tgccgattac ttgaaaaacc aaggcttgaa agtagcaaag attcatggag
    15361 acatcacccc aagagagaga aaacgagtga tgcgacaagt acagaatctg gagtatcaat
    15421 atgttgtagc gactgattta gctgctagag ggatcgatat cgaaggtgtc tctcatgtta
    15481 ttaatgcaga aattccagaa gatctagact tctttatcca tcgtgtcgga cgtactggac
    15541 gtaatggctt aaaaggaacg gccattacgt tatattcgcc aaatgatgag caagccattg
    15601 atgcgataga aaaattgggc gtctcatttg aaccaaaaga aatcaaaaac ggggaaatcg
    15661 tcaaaacata cgatcgtaat agacgtgcca aacgagaaaa atcaaaagat gtattagatc
    15721 ccacgctgat tggtcttgtg aagaagaaaa agaaaaagat caagcctggt tataagaaaa
    15781 aaatcgattg ggcaattgca gaaaaaaata aaaaagaacg caaaatcgaa cgcagacagc
    15841 aaagccgtac tgctcgtaaa aataagaaaa attcacataa ataaaaaaca agaggttgtg
    15901 acaaaaggcg tgtcacaatc tcttgttttt aagctagact ttttttcatt ccgatacaaa
    15961 gtacttcatc ttcaataaaa ggttctgtgc aagtatgata cccttgtttt tcataaaaat
    16021 ctacagccgt catttcagct actacataac tagtctgaca accttctgaa accgcccgtt
    16081 tttcagcagc ttcaagcaac tgaccgccta acccttttcc tcggtattct tttgctacgc
    16141 aaaatcgttc cggcttgaag caagtaggtg ttttcttttc ataacgcaaa gtagctactg
    16201 gtcttttttg atcaagaaat agataatagg tttgttgcgg gatatctttt ccgtcgaatt
    16261 ctagttctgc aggaatccct tgttctaata caaaaacttg atatctcaaa aagcagctag
    16321 ctgcgcgtat ccattcatcc gctccaacta tttttttcat catattcctc cttgtcatga
    16381 atagcagaga tgtattgatt cctttattct tatcgtacca aaaatcgtgg agtatgacca
    16441 aaaggaaatg aaaataaaaa ttgagatatt gacagtgatc aatagaatgc gtatactaat
    16501 taagtacgaa aaggatatga tttgtttttt aagactcaca gaaaattctt tcttggctga
    16561 aaaaagaata gtcactaatc aaattgctcc ctttttctat tttggtagaa ataccgacgc
    16621 agctcagcgt tatcgagttt aagaggtaat gattacacat cattgcaatc aaggtggtac
    16681 cgcgagtttt cgtccttggc tgtgatggtg tttttttgta tttaatttat agaaagcagg
    16741 taatgacatg aaacaattaa gtagtgcaca agttcgacaa atgtttcttg atttcttcca
    16801 atctaaagga catacgattg aacctagtgc gtctttgatc cccgtgaacg atccaacctt
    16861 attgtggatc aactctggtg ttgcaacatt gaaaaaatat tttgatggtt cagttgtgcc
    16921 agaaaatcca agaatcacaa atgcccaaaa atctattcga acaaatgata tcgaaaatgt
    16981 cggtaaaaca gcacggcacc atacgatgtt tgagatgctg ggtaattttt cgattggtga
    17041 ctatttcaaa aatgaagcca tccactgggc atgggaattt ttgactgatc caaaatggat
    17101 ggcattcgat ccagaaaaac tttatgtaac agtttatcca aaagatacag aagcaaaacg
    17161 catctggcat gaagaagttg gcctgccact agatcacatt attgatgtag aagacaactt
    17221 ctgggatatc ggtgcaggcc catgtggtcc ggatacggaa attttctatg accgtggaga
    17281 agcttataac gatttagcag aagacgatcc tgaaaactat ccaggaggag aaaatgaacg
    17341 ttatttagaa atctggaact tagtattttc agaattcaac cataagcctg ataacacatt
    17401 tgaaccactt cctcataaaa acattgatac ggggatggga ttggaacgca tggtctcaat
    17461 cgttcaagat gcgtcaacga actttgagac agacttgttt atgcctatta tccacgccgt
    17521 agaaaagcta agcaaacaag taaaatatgg tgaaaaccca gtaacagata tttcttacaa
    17581 agtaatcgca gatcatattc gcgccctttc atttgctatt ggcgacggtg cattgccatc
    17641 gaatgaaggg agagggtatg tcttacgccg tttgcttcgt cgtgctgtca tgcatgggaa
    17701 aaaattaggt attgaacaag cattcttata tcgtttagtc cctgttgttg gggaaatcat
    17761 ggtaagttat tatcctgaag tacttcaaca aaaagaattt attgaaaaag ttatccgtac
    17821 agaagaagaa cgtttcaatg aaacaatcaa tgaaggtttg gaaatcttaa atcaagtgat
    17881 ccaagaagta aaaaacgaaa acgggacgac tctagcagga aaagacatct tcaaactata
    17941 cgacacatat ggcttcccgg tagaattaac ggaagaggtg gcagaagatg aaggactgaa
    18001 agttgatcat gctggttttg aaaaagaaat ggatgcacaa cgcgaacgtg ctcgctctgc
    18061 ccgcagtact gaaaaatcaa tgggggtaca atctgcttta ttgacagata tcaaagtaga
    18121 aagtaaattc attggataca cagaacaaga atcagatagt caattattgg tcatcgttaa
    18181 aggtgagtct cttttgaatg aagtcgcaga aggtgaagct caaatgatct ttgatcaaac
    18241 acctttctat gcagaaatgg gtgggcaagt agcggatcaa ggatggatca tgaatgaagt
    18301 aggcgaaatc gtagcagttg ttacagatgt tcaaaaagcg cctaatggtc aatttatgca
    18361 caccgtagaa gtaaaagcac caatgagtga aggacaaacc tatcatctat tgatcgatca
    18421 atcgatgcgc aatcggatca tcaaaaacca tacagcaacc catctactgc acaaggcatt
    18481 gaaagaagta cttggcgagc atgcaaatca agctggttct ttagtcgctc ctggacattt
    18541 acgattcgac tttacccact ttggtcaaat cacttcagat gaattagctg aaatggaaag
    18601 aatcgtcaac gaaaaaatct gggaagctat cccagttgaa acaattgaaa cagatatcga
    18661 tacagccaaa aacatgggtg caatggcttt gtttggtgaa aaatacggac atgacgttcg
    18721 agtagtaaat attggcggct ggtcaatcga actgtgcgga ggcacacatg tagcaaatac
    18781 agaaaacatc ggaatcttca aaatcgtttc tgaatcagga attggtgcag gcgtgcgacg
    18841 aatcgaggct gtaacaagta aagaagcgta cgaactactt gaagaagaag aaaaacaact
    18901 aaaacaagta gcaacaattg ttaaatcacc tcaattgaaa gaagttgttt caaaagcgga
    18961 acaattgcaa cagcaagtaa gagagctaca aaaggaaaat gaacaattag ctagcaagtt
    19021 agcgaatcaa caagctggtg atgtattcaa agatccagaa acgatcaatg gaaaaacaat
    19081 cattgcagca caagtaaaag tgaaagatat gaatcagctg cgtcaattag ctgaccaatg
    19141 gaaacaaaaa aatatttcag atattcttgt tttagcaaac gaacaagaag gaaaagtcag
    19201 tctgctagcg gcaatgacga aagaatcaaa tgaagctgga ctaaaagctg gcgacttgat
    19261 taaagcaatc gcaccgaaag taggcggtgg cggtggtgga cgtccagaca tggcacaagc
    19321 tggcgggaaa aatcctgctg gtattccaga tgcacttaaa gctgcgaaag aatgggttgc
    19381 gcaacaatag ggcgtttacg tttgacagaa aaacaaaagt tcaaacattg aacgaaacat
    19441 gattatgggg gcgaagacaa acaagtcttg ccctcatact tttttcaatt ggatggcgca
    19501 tctaatcaaa tggtcggatt cggatggtct ttagttgacg aaagtgacag aaatttttac
    19561 gattagtata aataaaaagg ggtgaaaaaa tgaatagtag aaataagacc tttcgactag
    19621 tcttacgtgc gatcttgtta gcaatcatca tcgtacaagc gatggttcct tggctggggt
    19681 ttattccatt aggattcatt agtttaacaa ttatccatat cacagtaatt attgctgcag
    19741 tagtattagg cccaaaagat ggcatggtga ttggcttgtt ttggggaatc gctacaattg
    19801 ttcgtgccta tgccatgcca acaacgccat ttgatacatt agtgttcacg aatccgatta
    19861 tctcggtagt tcccagagta cttgttggct tagtagcagg cttggttttc cactggattt
    19921 atcaaaaaaa taaatcaata acgatcagct cagcctttgc aggggtactg ggttcattgg
    19981 tcaatacagt cttagtgtta ggattcatgg gattatttta tacaggtgca acggcagatg
    20041 cctatggtgt agactctgct cttttattta aaaccttagc aggggttgca gcaatcaatg
    20101 ggataccaga agcaataggt gcgggaatca taacaccatt actagcaaaa gcgttatttg
    20161 cagcaacacc gttgaaacca gaataatgac catcttttaa tagtaatagg acgcacgata
    20221 gatagaaaaa ggaagagagg agaggaaaac aatcgtcctc tccatctttt tacctgagca
    20281 acaagcaaac tagaaggggt aaaaaacttg gctaagaaac aagaagaaaa aaagaaacaa
    20341 ataccgcaac agcggacaaa aaaaggagta ctagcgggta tcttaggatt ggttacgaat
    20401 ctgatcttgt ttgtctctaa gctaatgatc gggctttttt caggaagtgt ctccattatg
    20461 gctgatgcag tgaacagtct atcagatact gcttcatcca tattgacttt gatcggattt
    20521 aaaattgctg caaagccagc agatcaggaa catccttatg gacacgaacg cttcgaatat
    20581 atcagtgggt tatttgtttc gatcatcatt acttatgtag gatttcaatt tttagagtct
    20641 tctgctgaaa aaatcatcca tccagaaaat gtctctttaa cacccatcgt cttttttgtc
    20701 ctaatcattt ccatcttttt gaaatttgca caaggcaaaa tgtatcaggt tattgcgcaa
    20761 tccattgatt ctgaaacatt gagagctaca gcaacagata gttataatga tgtctttact
    20821 acactggctg tattgctttc agcagcaatc gaatgggtaa ctggatggcg tattgatgga
    20881 tatataggtt ttgctttagc attatatatc ctttatagtg gattgacgat gcttcgaaat
    20941 tttgtatacg aactattagg aagtcgccca acgccagcag agattgaaga aatggaagag
    21001 catttaaatg cttgcgatga tatacttggc tatcatgatt tattggtgca taactatgga
    21061 cccaataaac gctttgcatc agtccacatc gaagtagatg aatcaatgaa tttgaatgtg
    21121 gctcataaga ttattgatct gatagaaaaa gactttaaaa agtctttgga tgtggaactt
    21181 gtctgtcact tggatccaat gcctgttgat aacgaaaaat actataatat cttgcggcag
    21241 ttaaagcaaa ttcttcttga aacaggtaca gggcttaata ttcatgattt tagagtaatt
    21301 catcaaggaa aaacgctgca atttgatgta gtggtccctg agaaattcca gtttgttgat
    21361 acagaactag aaagaatctt acgtagtcaa atagaagaaa aaatcggaga ttaccaattg
    21421 gaaattacgt ttgatcacaa ttatctttta tattaaatga gaaactataa aaagactcct
    21481 taaagttttt aatttaaaga gtcttttttt cttttaattg tttatggaga gcctgacgtg
    21541 caagattatc tgctccttta tttttgtttt cgggcaacca ttgcaggatc aataaagaaa
    21601 attcggatag aagttcttgg attctttgaa ggtagggaag gaagagctcg ttcttcgttg
    21661 cgtttttgtc gaccgtagaa actagtgttt tgctgtctga aaaacagaaa atcgtttcat
    21721 tctgtaagtc gtttttcttt agaaattcta aagtttttaa aaagacttcg aactctgctt
    21781 gatggtttgt ccctatagaa agcgggatag atagctgttc ttgtttacct tgatagacaa
    21841 tcaagattcc acctccacta ggaccagggt tgccttttgt agaggcatca atgtatactt
    21901 ttaacataaa aagagattct cctttttgtt caaactaatg ttatggtaag attatatcat
    21961 tactgaatcg gtgagaggtt agagaatgaa aaaaaatatt cgatggcagc ctgaactggc
    22021 acatacgatc atctattggt cattgacctt gatcattctg ttttttagtc tgattctttc
    22081 tttagaaaac acaggtcctt attggaaaag caattttgtt ttaggtattt ttcttgtttt
    22141 tctatttcta ggtttgcggc gttcttttat tttaaaatca aactctgtga ttatacgtta
    22201 tgctgctttt tggaaagacc gagaaatatc tgtcggacag attcgatctt tgacgccaac
    22261 taatactggg gtgcaaatga tgttgcaaga tgagaaagcc ccatctgttt tcatgatgcg
    22321 aaaaaaagat caagtagtcc tttttgatca ttttgaaaaa aacggaccca aatcagaatc
    22381 aattccttat ccttatacga aaaaaagaaa aagctaattt taaaaatcag aacctaaaaa
    22441 aacgaatttt tacaggaaaa gaaaagagtg agacgtacgg ttcatcccgt ttagcgtctc
    22501 actctatttg tttatctatt catgcatact agtatcggta gtctgaacgt tgcctttctt
    22561 gacatgggca gcttgtgttc ctcgattccc ttcgatgatc tcaaattcga ccacttgatt
    22621 ttcttccaaa gttttgaatc cctcttcttc aatcgcagta aaatgtacaa aaatttcttc
    22681 tgtgtcatca taactgataa agccgtatcc ttttttgttg tcaaaccatt ttacaattcc
    22741 tgtggtcaaa gcactcacga cctttctaaa gtctgatcat agccttcatt ataaagagta
    22801 tttataagta cgtcaaataa attaaaaacc tgtaatttta tgtttttttt ctgaataaaa
    22861 aaaggtataa ttggcatatc ttaacaaaga agatattttg ttgaataaag ttcgtgaaaa
    22921 ggggtaatca aatgaagaat gatctgcaaa tttcacaaga aacagagcta gaaccgattg
    22981 ttgaagtggc tagtaaatta gatcttaagg aagacgattt ggaactgtat gggaaatata
    23041 aggcgaaaat caatttttct ggaatcaatc gatcaaagga taacgctgag gggcatttga
    23101 tcttagttac ttccatcaat ccaacaccag ctggcgaagg aaagtcaacg atcactgtag
    23161 ggttaggaga cgcgctcaat aaaatcaata aaaaatctgt tattgcattg cgagaaccct
    23221 cacttggtcc tgtgatggga atcaaaggtg gtgctgcagg cggaggatat gcacaggtat
    23281 tgcctatgga agatatcaac ttgcatttta ctggtgacat gcatgctatc acaacagcta
    23341 ataatgcatt atctgctctt ttagataatc atattcatca aggaaatgaa ttgaacatcg
    23401 atgcccgccg agtgatttgg aaacgggtag tcgatctcaa tgatcgtgaa ctgcgtaaag
    23461 taattgttgg cttgggtggg ccaatccaag gtgttccaag agaagatggg tttgatatca
    23521 cagttgccag cgaaatcatg gctatcttgt gtcttgcggc agaccttgaa gatttaaagg
    23581 ctcgtcttgc aaggatcgtt gtcggctata catttgaccg tcagcctgtg actgctggag
    23641 acttgaaagc agaaggcgct ctggcattac tgctgaaaga tgcaatcaag ccgaatcttg
    23701 tacaaacaat ttacggaaca ccagcttttg tccacggggg accgtttgcc aatatcgctc
    23761 atggctgcaa tagtatttta gctaccaaga cagcattgag attagcagat tacgttgtca
    23821 cagaggcagg atttggtgca gatcttggcg gagagaaatt tttagatatc aaagtaccta
    23881 acttgaaaaa agcgccggat gctgtggtga ttgtcgccac tgtccgtgca ttgaaaatgc
    23941 atggcggaat gaaaaaagat gaacttaaaa atgagaatct tgatgctttg aaagtaggtt
    24001 ttgcaaattt aaagcgccat atacgtaata tggaacaata tcagctacct gtgatcgtcg
    24061 caatcaatga attcgtaaca gatacagaca atgaactcac actgttggag cacttgtgtg
    24121 aaaatcaggg gattcttgcc aagagagcga gtgtatgggc agatggagca gaaggcggcg
    24181 ttgatttagc aaaagctgta gtacgattga ttgatagaaa agaagctaat tacaagccat
    24241 tataccgcct agaagaaacg atacaagaaa agacagaaac aatcgtcaag aaaatttacg
    24301 gaggaaatgg tgttgttttc agcaaaaaag cgcaaaaaca aatagaagag tttacgaaga
    24361 atggatggga caaactgccg atttgtatgg caaaaacaca gtattcattt tctgatgatc
    24421 cttcactgct aggtgcaccg gaagatttca ccatcacgat ccgcgaaatc atcccaaaat
    24481 taggggcagg atttttagta gccttgactg gagatgttat gacgatgcct ggtttgccta
    24541 aaaaacctgc tgcattgaat atggacgtga caaatgacgg agaagtgttg ggattgtttt
    24601 aagcttgatt ataatctacc atcgaaggag ctgtattttg actacagctt ctttttttag
    24661 acgataggga attaattttg tttacactag gatcattctt tttctttatt gtattttgag
    24721 ctatactaaa gcataatagg agggtatgat gaaaacgaca tttataagaa aaaccggttt
    24781 ttacggtatg ggtagctcga ttcagctaag aatcaacaat gaaaaacgca gtgttctcag
    24841 tcatgaacaa aagataacgc ttgatctgga agtgcccttt acgatgcagg tcacctttta
    24901 ttggctaaaa agccccgttt atacagtgaa agaagcaaag cctgcttatg tgatcacaat
    24961 gaattacttg attcttcaaa tctatccgtt tttatttcta gcaactgggt tgggggccgt
    25021 tttcctacaa aatttatttt tcagtttttt tgcagttatt gtgttgattg cgttcttttt
    25081 ctatataaaa aaccgggcat atttgatcaa ggaggaaaat tatggcaaac tctgatcttt
    25141 ttttaatcag tttcaatcga atcgaaaaat ggatgagaga cgagatggga aatgcacgaa
    25201 atatgggatt taccgaactt gtcagacgcc tagctcaaag aaaacaatta atgatcagaa
    25261 aatacgaaga tgatctttta cagcttgctc agttaaggaa tgcgatcgtc catgatcgga
    25321 tcgcagtcga ttttgtcatt gcagagccaa atgattggac agtcaagcga atccagctga
    25381 tcgaacaaga actgattcag cctgaaaccg tgctgcctcg atttgcaaaa cgtgtaacag
    25441 gttttgaaca agatcttccg ttacttgaac tgttaaagat agtagcagaa aaaaggtatt
    25501 cacaatttcc gttatacaat aaaggaaaat ttgtagcatt gattacttta cgtaatatcg
    25561 ggttttggct agcaaaagaa agccaaaaag gtccggttga tttaacaaat aaacgagcgt
    25621 tggatttgat catccaaaac ggaaagtaca caaattatca ttttgttcca gctaaaacac
    25681 atatttacga ggttgaagcg atgtttcgtg agcaagggac tttggaagcc gtacttatca
    25741 ctaaagatgg gaatcccgat ggtaatctgc tcgggatcgt acgacctcgt gatatttata
    25801 aacaaataga aaaggattga ttttttgtta attatttatt ggatattcag cgctttgcta
    25861 gtaggagtgg atcagttggt caaatggtgg atcactgata atctagcgct tggagagacg
    25921 aaaaatctta taccaggtat tttgtcactg aatcatattc gaaacactgg agccgcatgg
    25981 agtatgctag aagggaagat gtggttcttt actatagtga cactcattgc agtcgttgtg
    26041 attctcacat tgatgattaa aaatcgtgaa aaaggtaatc gctggttcat gattggattg
    26101 agtttgattc tagctggtgc gattggcaat tttatcgacc gtattcgtct tggctatgta
    26161 gtagatatgt tccagacaga ttttatgagc tttccgattt ttaacgtagc agatgtgaca
    26221 cttgtatgtg gagtgatttg tatgttaatt tatattattt tagatgaaaa ggatcaaaga
    26281 aaaaaatgac agaacttaaa acgacgattc accatgaatc tggtaggatc gataaagtat
    26341 tgactaattt attcaccgat tacagtcgtt cgcaaattca gctgtggctg aaaaatggag
    26401 cggtttctgt caatggacag ccagtcaaag caaattacaa agtaaaacaa ggagacgaga
    26461 ttactgtctt gatccccgaa ccagaaacat tagatgtggt cccagaaaat atcccgttga
    26521 cgatcgttta cgaagatgaa gatgtagcgg tgataaacaa acctcaagga atggtcgttc
    26581 atccttcagc aggtcatacg catggcacaa tggtcaatgc gctgatgtac catatcaagg
    26641 atctttctac gatcaacggc gttattcgtc caggtattgt tcaccgaatc gacaaagata
    26701 cttccggact gctaatggtt gccaaaaatg accaggcaca cgaatcatta gctcagcaat
    26761 taaaagataa gacctctttg cgcaaatatg ttgctttagt ccatggagag attcctcacg
    26821 aaaaaggaac catcaatgcg ccaatcggcc gttcgaagga aaaccggaaa atgcaggcga
    26881 taagagaaga tgggaagtcg gctgtcacac attttactgt attggaacgt ttcgaaggat
    26941 ttactctggt tgaattaaaa ctagaaaccg gaagaacaca ccagatccgt gtgcatatga
    27001 agtatatcgg ttatccgtta gctggagatc cagtatacgg accgaagaaa actttgaaag
    27061 gcaatggtca gtttctccat gcgcaaatgt taggttttac gcacccgact acaaacgaaa
    27121 agatgatctt tgaagcacct cttccagaag tttttgaaaa aactttagaa aaattaagaa
    27181 aaaacattgc attttaattt agataacggt atagtaatta acaacgaaag cataaaactt
    27241 aatagaattc ctttaagttt agtcccgtga ggctaagaag ggtgggagca aggatacgta
    27301 tggtgcgcca taagtagatt gctcattgac atagtatagc tatcagtgta tcagtatacg
    27361 ctcagtctaa acattcctcc tgtcctctta gggcaggagt ttttttatgc aaaaaagtag
    27421 ggggaatgaa aatgcaagca aaagaagtag tagatcaggt aaccatgaag cgagcattga
    27481 ctcgaattac ttatgagatt atcgagagaa accatagtat ccaagatatc gtattggtag
    27541 gtatcaaaac acgaggaatc tacattgcat caagaattgc cgaacgcctg aagcagctag
    27601 aagatatcga tattcctgta ggagaactgg atatcacttt ataccgagat gacaaaaaag
    27661 aaaatccaga agaaccggaa cttcattctt ctgacatccc agtttcctta gaaggaaaag
    27721 aagttatttt gattgatgat gttttgtata caggacgaac gattcgagca gcaatggacg
    27781 cagtaatgga ttttggcaga ccaagaaaaa tttcattagc agtcctggtc gatcgtgggc
    27841 atcgagaatt gccaatccgt gcagattatg ttgggaagaa cattccaaca tctcgggcgg
    27901 aagagatttt agtagagatg caagagcttg atggacaaga tcgcatcatg attttgaaag
    27961 aggaagatta gaggagagag aaacatgtcg gagaaaaaag gatttcataa tgatgacgtg
    28021 gtgttagata tccacgatcg cccaacccca ggacattggg taggattaag tttacaacac
    28081 ttgttcacga tgttcggagc aaccgtgtta gtccccatct tagtaggtat cgatccaggt
    28141 attgcgctag tcagttcagg actaggtaca ttggtgtact tatttactac taaaggaaag
    28201 atacctgcgt acttaggaag cagcttcgct ttcattgcag cgatgcagct tttgatgaaa
    28261 acagatggtt atccagcgat tgcacaagga gcagtcacaa ctggattagt ctatttgatt
    28321 gttgcgctga tcatcaaaaa gatcggttca gcatggctgg ataaaatctt gcctccaatc
    28381 gtcgtcggtc cagtcatcat ggttatcggg ttgggacttg cggcaaatgc agcaacaaat
    28441 gctatgtata atcagggcac gtatgatttc aagtatattg ctgtagcgct gatcacatta
    28501 ggattaacaa ttttcttcaa catgtggcta agaggttttc tcggattgat tccgatttta
    28561 ctaggtatta ttagcggata catcgtagcc ttgctgtttg gaatcgtgga tactaagcca
    28621 atcgtagacg ctgcctggtt tgctgttcct aactttgaga ttccctttgt acaatatgaa
    28681 ccaaagctat atcttggagc aatcacaaca atggcaccca tcgcttttgt gacgatgaca
    28741 gagcatatcg gccatcttat ggtattgaat aaactaacta aacgtaattt ctttgaagaa
    28801 ccgggtcttc ataagacgtt gacaggtgat ggattagcac aaatcgtggc agcctttgtc
    28861 ggaggtcctc ctgtaacaag ttacggtgaa aacattggtg ttctggctat cacacgtgtc
    28921 catagcgtgt ttgtgatcgg tggagctgca gtatttgcag tcattttagg attcgtagga
    28981 aaattaagcg ctttgatttt aagtatccct ggaccagtca tctcaggaat cagttttatc
    29041 ttatttggag taattgccgc aagtggaatg aagattttaa tcgagaacaa aatcgatttt
    29101 gataaaaaga aaaatctgtt gattgcttca gttatcttag tagtaggaat tggcggactc
    29161 gtctttgaag taggcacatt tacattgtcg gcaatggcat tagcaactgt acttggcatc
    29221 attttaaatc ttatcttacc agaaacttca cgaagcgaag aaaactaagg aggaaacatt
    29281 atgatcatca aatcagaacg tatcagctta aagcatttat tgaccgttga ggcacttact
    29341 gatcaagaag ttatgggctt gatccgcaga gggcaggaat tcaagcaagg tgctaattgg
    29401 actccgcaaa agcgccagta ctttgctaca aacctctttt tcgaaaatag tactcgcaca
    29461 cataaaagct tcgatgtagc ggagaaaaaa ctagggctag aagtgattga atttgaagca
    29521 agcacaagct ctgtccaaaa aggggaaaca ctatacgata cagtactgac gatgtcagca
    29581 ttaggcgtag acgtagcggt tatccgtcat ggtgatgaga actattatga cgaactgata
    29641 caaagtaaaa caatccagtg ttcgattatc aatggaggag acggaagcgg acagcatcca
    29701 acccaatgct tgcttgattt gatgaccatt tatgaagaat ttggtcattt tgaaggtctg
    29761 aaagttgcca tcgtaggtga tatcactcac tctcgcgtag ctaaatccaa tatgcagatg
    29821 ttaaaacgtc taggcgcaca agtattcttc tcaggtccta gagaatggta cgacgatgaa
    29881 ttcgaagtgt acggacatta tatgccattg gatgaactac tagatcaagt tgatgtcatg
    29941 atgatgcttc gggtgcaaca cgaacgccat gatggaaaag aaagtttctc aaaagaaggc
    30001 taccatcagg aacatgggtt aacagttgag cgtgcgaaga agttgcaaaa acatgcaatc
    30061 attatgcacc cagcaccagt taaccgtgat gtagaattag ctgattcatt agtggaagga
    30121 ttacagtcgc ggatcgttca gcaaatgagc aatggagtat tcgtcagaat ggcaatctta
    30181 gaagcagtgc taaatggaaa agcgtaatta taggaggatg aaaaaatgaa aacactcatt
    30241 aaaaacggtc agatcaatac acgaaaaaat atgacgacac cagcagaaat ttggatagaa
    30301 gatgggcgaa tcaaagcaat cggtacagga ttctcagaag ccgaatttga tgaagtgttt
    30361 gatgcaaagg gacaattgat cacacctggt ttagtggacg tccatgttca tttgagagag
    30421 ccaggattta catacaagga gacgatcgaa gctgggactc gctcagctgc tcgtggcggt
    30481 ttcactactg tatgcgcgat gccgaattta aatcctgtac ctgatacggc agaaaagctg
    30541 agacaagtat atgacatcat tagaaaagat gcagttgtga aggtattgca gtatgcgcca
    30601 atcacagaaa atctgcgaag tgagaaatta gttgatcaag aagcactgat tgaagaaggt
    30661 gcttttgctt tcacaaatga cggtgtaggt gtccaaacag ctgggacgat gtatctggct
    30721 atgaaagaag ctgcaaaaaa caataaagcc ctagtagccc atacagaaga tgaatcttta
    30781 ttatttggcg gcgttatgca cgctggcaag aaggcagaag aacttggtct tcccgggatc
    30841 ttaagcgtaa ccgagtcttc tcaaattgct agagatctgt tgctagctga agcaactggt
    30901 gttcattacc atgtttgcca tgtttctact aaagaaagtg tccgtgtaat cagagacgca
    30961 aaaaaagcag gaatccacgt aacagcagaa gtttcacccc atcatcttat tttgatcgac
    31021 gaagatattc ccgaagattt cggcttttgg aaaatgaacc cgccgctaag aggacgggag
    31081 gacagagaag ctttgatcga aggcttgctt gacggtacga tcgattgcat cgctacagac
    31141 catgcccccc atggattaga agaaaaaagc caaagtttca tgaaatcacc atttggtatc
    31201 gtaggaagcg aaacagcgtt tcaactgatc tatactcatt ttgtagaaac tggtcgattc
    31261 acgttggaac aagtgatcaa ttggttggca gttaaaccgg ctgagatttt tggtttgaat
    31321 gcaggaacgc taacggttgg tgcccccgct gatgtagcag tttttgatat tacacaaact
    31381 tgcacgatcg ataaagaaga ctttttatca aaaggggaga atacaccttt tatcggctgg
    31441 aaagtaaaag gcgaaacaca aatgactttt gtcaacggaa aacttgtgtg gcagaaggga
    31501 gaataaaagt atggaacgtt tgcttatatt agaagatgga acagttttcg aaggaaaggc
    31561 ttttggtgca gatgctagtg tcttcggaga aatcgttttt acaacaagta tgactggtta
    31621 tcaagaaact atcactgatc aaagttttaa cggtcaaatc attacattta cctatcctat
    31681 ggtgggtaat tatggggtaa atagagatga ttatgaatcg atttctccaa catgcaaagg
    31741 agtagtagta aaagaacatg cacgtgtagc ttcaaactgg cgaaaccaaa tgacgctaga
    31801 ttcatttttg aaacaaaaag gaattcctgg aatatcaggt atcgatacgc gcgcccttac
    31861 tcgcaaaatc cgtgaagtcg gaacgatgaa agctagtata gttgatgctg gagattcctt
    31921 cgaacatgca tttgatcagc tgaaagcaac cgttctagca accaatcaag tgcagcaagt
    31981 ctcaactaac aaaccttatc caagtccagg aacaggtcgg aaagtcattg tcattgactt
    32041 cggcttgaaa catagtattt tacgagaatt gagtaaaaga aactgtaacc tcacagtact
    32101 tccctataac acagatgccg agacgatttt agcgctatca ccagacggag tcatgctgac
    32161 gaatggacct ggtgatccaa aagatgtgcc agaagcaatc gaaatgatca aaaaaatcca
    32221 aggaaaagta ccgatttttg gtatttgtct cggtcaccag ttatttgctt tagccaacgg
    32281 tgcagatacc tataaaatga aattcggaca tcgaggatta aatcacccgg tacgagaagt
    32341 agcgaccgga agaattgatt ttacttctca aaatcatggc tatgcagtcg atgaagccac
    32401 gattgatcca gaaaaactca tcgtaactca tgtagaagta aatgacggaa cagtagaagg
    32461 agtcaaacac agagattatc ccgctttcag tgtgcagttt catccagatg ctgcccctgg
    32521 tccgcatgat gcactacatt tatttgatga atttatggaa atgatggacg caggaaagga
    32581 gcagtaaaca gatgccgaaa cgtacagata tcaagaaaat tatggtgatt ggatctggtc
    32641 cgatcatcat tggtcaagcc gcagaatttg actacgcagg aacacaagcc tgccttgcct
    32701 taaaagaaga aggatatgaa gtggttttag taaattctaa tcctgcgacg atcatgacgg
    32761 ataaagaagt ggcagatcaa gtatatatcg aaccgatcac acttgaattt gtatcaagga
    32821 ttcttcgaaa agagcgtcca gatgctctcc ttccaacttt aggcggacag acaggcctca
    32881 atatggcaat ggagctggca gcatcaggta ttttagaaga attgaacata gaacttctag
    32941 gaacaaagct ttctgcgatc gatcaagcag aagatcgtga tctattcaaa cagttgatga
    33001 aagaattgaa tcagccaatc cccgagtctg aaatcgtcaa tacagtagaa gaagctgtag
    33061 attttgcaaa taaaatcggc tatcctgtca tcgttcgtcc agcctttact ttaggcggaa
    33121 ctggtggagg tatgtgtgaa aatgaagaag aattacgtat cgtatcagaa aatggattaa
    33181 gtctttcccc cgctacccaa tgtttgattg aacgaagtat tgcaggtttt aaagaaattg
    33241 aatatgaagt gatgagagat tcggctgata atgcaatcgt tgtctgcaat atggaaaatt
    33301 ttgacccggt gggtatccat acaggtgatt ctatcgtatt tgcccctagt caaacattgt
    33361 ctgaccatga atatcaaatg cttcgtgatg cttctctttc gattattcga gcacttaaga
    33421 tcgagggagg ctgtaatgtc caactcgctc ttgatccaca tagttttaat tattatgtga
    33481 ttgaagtcaa tccgcgtgtt tctcgttcat ctgcgttagc aagtaaggcg actggctatc
    33541 cgatcgctaa gctagctgct aaaatagcgc ttggattaac attagacgaa atgaaaaatc
    33601 ctgttacagg aacgactttt gcagaatttg aacctgcatt agattatgtt gtagctaaaa
    33661 ttcctcgctg gccgtttgat aagtttgaaa acggagaacg gcttttaggc acgcagatga
    33721 aagcaactgg tgaagtgatg gctatcggtc ggaatatcga agagtcttta ttgaaagctg
    33781 tgcgttcttt agaaatcggt acacaccata tcgaacttcc tgagttggca gacatcacgg
    33841 acgatgacct aatccaaaaa gtggttcgag cacaagatga tcgcctgttt tatgtagctg
    33901 aagctattag aagagggtac accatagaag aattagcgga tttgacaaaa atcgatttat
    33961 tctttttaga taaactgttg catatcgtag aaatcgaaca gcagttgaaa cagcagccgt
    34021 tggatgacaa gatattgaaa acagcgaaac aaaacggttt tacagacaaa aaagtggcgg
    34081 agctgtggga aataaacgaa gaagccgttc gccagatgag aaaagaaaat gatttgattc
    34141 ctgtatataa gatggttgac acttgtgcag cggaattcga atcaaaaaca ccttattttt
    34201 atagttccta tgaatatgaa aatgaaagtt cacgatcaga aaagccctca attctagtac
    34261 tgggatctgg tcctatccgt atcggacaag gggttgaatt tgactatgcg actgtgcatt
    34321 ctgtcaaagc aatccagcag gcaggatacg aagcaatcat catgaacagc aatcctgaaa
    34381 cagtttcgac tgacttttct atttcggata aattatattt tgagccattg accttcgaag
    34441 atgtgatgaa tgtgatcgag ctagaacagc cgataggagt aatcgttcaa tttggtggtc
    34501 agacagcaat caacttagcc gaacctttga ctgctgctgg tgtaaaaatc ttagggacaa
    34561 cgatcgaaga cttggatcgt gcggaaaatc gtgatttatt cgagcaagcg ctgaaagagt
    34621 tggatattcc tcaaccttta ggggatactg ctacaagtaa agaagaagcc gtggttatcg
    34681 cttcgagaat tggttatcca gttcttgttc gtccaagcta tgtattaggc ggacgtgcga
    34741 tggaaatcgt tgaaaaccag cgagatttag aagattacat ggaacatgct gtgaaagcat
    34801 ctccagaaca tccagtacta gtggaccgtt acttgcttgg tggtgaatgc gaagtggatg
    34861 cgatttgtga cggtgaaaca gtactcatcc cagggatcat ggagcatatt gagcgtgctg
    34921 gtgtccattc aggggattca atggctgtgt atccacctca aacattatct gaatcgatca
    34981 agaaaacgat cgaagattac acgatccgct tagcattagg tttgaattgt gtcgggatga
    35041 tgaatatcca gtttgtcatc catgaaaatg aggtttatgt gatcgaagtc aatcctcgag
    35101 caagtcggac tgttccattc ttaagtaagg tgacaggaat tccaatggca caaattgcga
    35161 cgaaagctat tttgggagag aaactaaaga cacttggtta tgaaaacgga ctttatccag
    35221 aaacaaaaga agttcatgtt aaagcacctg tcttttcttt tacgaaattg cagaaagttg
    35281 atacttactt agggcctgaa atgaaatcga ctggagaagt aatgggatct gacagacaat
    35341 tagacaaagc tttgtacaag gcattcgaag cttctggatt acacttgcct gaatatggag
    35401 ccgtattatt tacgattgcg gacgaaacaa aagaagaagc actagaatta gctaaacgtt
    35461 ttacagaaat tggctatagt ctgataggaa cgaaacatac agctgcttat ttcgaaaaaa
    35521 atggtctggt cgttgaaaca gtagcgaaaa tttctgagga agcagcagaa aaaaatgtag
    35581 tcgacttaat aagagatggt aaaacacaag tggttgtcaa tacgattgat aaagatcggg
    35641 gaaatgcttc taaagacggt ttcatcatcc gaagagaagc agttgaacat ggtattccat
    35701 tgtttacttc tttagacaca gcagatgcga tcgttcgagt gatggaatcc cgtgcttttt
    35761 caactaaccc tatttaacgg cgagcacatg ttaagacaaa aagacgaaaa accgttgcag
    35821 gaggggacag atgaaacaag aaatgatgga aatcgttcag caaaaacagc tggcaccaaa
    35881 gatttttgaa atgacattga aaggtgatct agtaaaagaa atgcagcaac caggacagtt
    35941 cttgcatatt ctcgtgccgc gtgcagactt gctattacgc cgaccgatta gcttgaatca
    36001 tatcgaccac gaaacaaata cttgccgtat catttatcgg gtggaaggtg aggggactag
    36061 agtcttctct actctttctg ccggagacca tttagatgtc atgggtcctt taggcaatgg
    36121 ttttgatttg tccaatcttc atacaggaga cgaagtattt ataatcggtg gaggaatcgg
    36181 tgtccctcct ctatatgagt tatcaaaaca gttagtcgaa aaaggaatta agccaacgca
    36241 ttttttaggt tttgccacac atgaagtcgt ttattatgaa aaagaattta atcgtctagg
    36301 agaaacaaga atcgctactg acgatggaac atacggtatg cacggaaatg tcggtaattt
    36361 attgttagga acgaagacgg aacctacagc tgtttttgca tgtggttcaa acgggttatt
    36421 gaagacagtg gaacagctgt tttcttcgca tcaaaatgtc cagttatcat tagaatcccg
    36481 catggcatgt gggatagggg cttgctatgc ttgcgtttgt catacgccgg aagatgaaac
    36541 aggaacaaaa agtgtgaaag tttgtgacga aggaccgatt tttaaaatag gagaggtaat
    36601 tatatgaatg agaatccttt agcggtcaaa cttcctggcc tagaattaaa aaatcctatc
    36661 atgccggcaa gcggctgttt cggttttggc aaagaatacg gaaaatacta tgatttgaat
    36721 cagcttggat cgatcatggt caaggcaacg actccaaatg cccgctttgg caatcccacg
    36781 ccaagagtgg cggaaacacc atctggcatg ttgaatgcaa ttggtttgca aaatcctggt
    36841 ctagacgttg ttatgcataa acttcttcca gaattagagg agtacaatga gttacccatc
    36901 atcgcaaatg ttgctggtgc ttgtgaagaa gactatgtag aagtttgtag caaaattggc
    36961 gaagcaccaa atgtgaaagc aatcgagctg aacatttctt gcccaaatgt caaacatggc
    37021 ggaattgctt ttggaacaga ttctgatgtt gcttttcaat taacacaagc agtaaagaaa
    37081 gtttcttctg ttcctgtcta tgtcaaattg tccccaaatg taacagacat cgttccaatc
    37141 gctcaggcaa tcgaagctgg aggggcagat gggtttacga tgatcaatac tcttcttggt
    37201 atgcggattg accttaagac aagaaaaccg attttagcca atcaaacagg tggactctcc
    37261 ggaccagcaa tcaagcctgt cgctattcgt ttgattcacc aagtagcaag tatatccagc
    37321 cttccaatca ttgggatggg cggcgtgcag acagtggatg atgttcttga gatgtttatg
    37381 gctggagcaa gtgctgtagc tatagggacc gcgaatttca ccgatccgta tatctgtcct
    37441 aaactaatta aagagctccc tgtgcgaatg agcgaactag gcatcgaatc gctggaaaga
    37501 ttaatcaaag aagtgagaga ggaacgtgaa cgatgaatca actagaccaa cgaccaatta
    37561 ttgcattaga tttttcaact aggcaagagg tagaagactt tcttcgattt ttcccgaaag
    37621 aagaaaaatt gtttgtgaaa atcggaatgg aattgttcta ccaagaagga cctgaaatcg
    37681 tacgttatct aaaagaggct ggtcataatg tatttttaga tctgaaactt cacgatattc
    37741 cgaatacggt cgaaaaagca atgcggggat tggcaaagct tggtgttgat ctcacctgtg
    37801 ttcatgccgc tggcggtatt cgtatgatgg aggcggcaat gcgaggatta gaagagggga
    37861 ctccagaagg tggaaaacgt ccgctacttc tagctatcac tcagcttact tctacaagtg
    37921 aagaagaaat gcatgccgat caactaatcg aagtttcttt agaaaaaagt gtgatccatt
    37981 atgctagctg tgccaaaaaa gcaggattag atggtgtggt ttcttctgct tgggaagtag
    38041 aagcgatcaa agaaatggca ggagacgaat ttgtttgctt aactccgggt ataagaccag
    38101 aaggaacagt cgcaggagat cagacaagag tagtcacacc ttctcaggca aaaaagatcg
    38161 gctcaacttt tatcgttata ggtcgaccaa tcactcagtc aactaatcct tataaagcat
    38221 atcaaacgat tcaaacagaa tggagctaac ctaaaatgaa tgttgaacaa agtatcgcaa
    38281 aagacttatt agaaattgaa gcagtatttt taaatccaag taatcctttt acatgggcaa
    38341 gtggaatcaa aagcccaatt tactgtgata accgaatcac gatgagctat ccaaaagtcc
    38401 gaaaagagat cgcaaaagga ttagcaagca aaatcaaaga agcttaccct gaagtacaag
    38461 tgattgctgg aactgcaaca gcaggaatcc ctcatgccgc atgggtagcg gaaatactgg
    38521 atttgccaat ggtctatatt cgaagtaagg cgaaagatca tgggaaaggt aaccaaatcg
    38581 aaggacgtat atttgaaggt cagaagatgg ttgtgatcga ggatttgatt tctacaggcg
    38641 gaagtgtctt ggaagcagct gaagcagcaa aaagagaagg tgcggatatc cttggtgttg
    38701 cggctatctt tacgtatgag ttgccaaaag gaaaagcaaa tttcgaaaaa gccgaaatcc
    38761 ctcttatgac gttgaccaat tattctgtat tgattgaagc agctttagaa gatcgttata
    38821 tcgatgagca agaattaact ctgctaaaag aatggaaaaa agatcctgaa aattggcaga
    38881 tttaagcgcg cttgatgaag atagagaaat ccgatttgtg tctccagaat catcaattgg
    38941 aatagcaaaa atccaaaaac tgattgcgag aagcatcttt tcatcaggta tgaaaagaaa
    39001 cttctatcgt gatcagtttt tgattttttt aaaattcatt tttctaagac tggttagcag
    39061 aaagaagaga ataatc
//
LOCUS       DACDPS010000018        36380 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.018, whole genome shotgun sequence.
ACCESSION   DACDPS010000018 DACDPS010000000
VERSION     DACDPS010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36380
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.018"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1076
                     /locus_tag="IWJ07_000989"
     CDS             <1..1076
                     /locus_tag="IWJ07_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP9332585.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
     gene            complement(1212..1406)
                     /locus_tag="IWJ07_000990"
     CDS             complement(1212..1406)
                     /locus_tag="IWJ07_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332586.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYPFV"
     gene            complement(1547..1882)
                     /locus_tag="IWJ07_000991"
     CDS             complement(1547..1882)
                     /locus_tag="IWJ07_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332587.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLINEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            complement(1912..3504)
                     /locus_tag="IWJ07_000992"
     CDS             complement(1912..3504)
                     /locus_tag="IWJ07_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332588.1"
                     /translation="MSKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTLEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEYGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTEREKIEYQRLNQAMEKYQAEPHKKTSGTLPGVFSISSDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEVGLKAAGRK
                     MMETLFEKEKLNDTGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNSLTKVVKEPVRYD
                     RHAMNLLNEVYKTLPDKRSEWNYGDENRTKLSPYTREKLDELTQYVLTKEPEYQEYIQ
                     ISKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVD
                     QTQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            complement(3524..3946)
                     /locus_tag="IWJ07_000993"
     CDS             complement(3524..3946)
                     /locus_tag="IWJ07_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332589.1"
                     /translation="MAKATRKHIYLYDMHIEVLDEIISTHDDISNYSEAVRHLCMMYD
                     PKTEEKISPSQIKLNAIGKEVSILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKTISESVTTKSEVFDPTRFLKR"
     gene            complement(3985..4263)
                     /locus_tag="IWJ07_000994"
     CDS             complement(3985..4263)
                     /locus_tag="IWJ07_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332590.1"
                     /translation="MEVKIRDIDEKTIQRIDRRCIELSERTGTKWSRNRYLKVLIEND
                     FDHALLDYKKDQFDRLLEKFVDIQTYNTKILEEYIATNNQLIALLIEQ"
     gene            complement(4540..4809)
                     /locus_tag="IWJ07_000995"
     CDS             complement(4540..4809)
                     /locus_tag="IWJ07_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332591.1"
                     /translation="MILLYRIVLIVGTELKILKKEQLFKVKEHHNQLLCIKTGTMNIQ
                     YRTEYLELTNQTDFLSIREQHKQEKLMATYSQLIDEWEQFKESKT"
     gene            complement(4853..5788)
                     /locus_tag="IWJ07_000996"
     CDS             complement(4853..5788)
                     /locus_tag="IWJ07_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HAP9332592.1"
                     /translation="MSTLSCVMLLYLCIQINEIMPPHWQEKKKEFKEEIEIKSNDWIE
                     YTWLPLKRNSKRKEFWALQLWPIGKLFIYMASLMVTLIGYLVVVYGVAYYLLSEPKQI
                     AASNIFTAVMTLMCVMCFSIFSYLLVQFIRQNLTMSKLFKSLLDLLILGSMLTTLTDR
                     FEQSEKTVEGIKNFLTTIDQFINGYMYLFEVGFILTLFGCVFIKLIKRSIEKEMVIDL
                     IPTLSGYEYYVDFGKYEELIHTDIVHTSIHEVTVKGIRSTLKDGQETKTIDRRKEVYN
                     FYKVEASLRYGIGKLRLDGSNIQQNKPYVLGKVVI"
     gene            complement(5947..6176)
                     /locus_tag="IWJ07_000997"
                     /pseudo
     CDS             complement(5947..6176)
                     /locus_tag="IWJ07_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6199..7005)
                     /locus_tag="IWJ07_000998"
     CDS             complement(6199..7005)
                     /locus_tag="IWJ07_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332593.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQIESFQATIDLNEELVKKLKDQLP
                     YQVAEDQENTENKINSYQQEIETSKTQMKDQEESIQKLQEKIDRMTKENI"
     gene            complement(7016..7660)
                     /locus_tag="IWJ07_000999"
     CDS             complement(7016..7660)
                     /locus_tag="IWJ07_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332594.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTYKIEEVAK
                     NASDKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPVDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            complement(7676..8875)
                     /locus_tag="IWJ07_001000"
     CDS             complement(7676..8875)
                     /locus_tag="IWJ07_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP9332595.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWVFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKLL
                     PSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFPN
                     SKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSAYYKNCMSII
                     NTNKLMEYDEFAIKHWREGGGTGGTITGSWGNPFPGSSLDKNSFSGGQLFGKNPGGEF
                     RPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVYQE
                     FANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDPLP
                     FLNSSKK"
     gene            complement(8875..10899)
                     /locus_tag="IWJ07_001001"
     CDS             complement(8875..10899)
                     /locus_tag="IWJ07_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP9332596.1"
                     /translation="MLDVIKTAFSTQSNQKVIDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLAFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            complement(10921..11514)
                     /locus_tag="IWJ07_001002"
     CDS             complement(10921..11514)
                     /locus_tag="IWJ07_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332597.1"
                     /translation="MRVKGQNSVDKKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            complement(11515..11847)
                     /locus_tag="IWJ07_001003"
     CDS             complement(11515..11847)
                     /locus_tag="IWJ07_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332598.1"
                     /translation="MDKYIHMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWLAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            complement(11925..12467)
                     /locus_tag="IWJ07_001004"
     CDS             complement(11925..12467)
                     /locus_tag="IWJ07_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332599.1"
                     /translation="MEDILIATFITALGAFLTIFVVTIKGFKDTDSLQKDHQSILDGQ
                     KTHTESIIVHLSEGFTYLKDAMPTFTVKLTEIFHSVEQISSELKEQRQQKDRQLGSLN
                     FRQETIQESLENVNRLNDEMQRVHLENEELRAQNYDLIQKKRNLEQKIVELKEENRQL
                     NYQLRRGKNRSRDLGNELEL"
     gene            complement(12528..14906)
                     /locus_tag="IWJ07_001005"
     CDS             complement(12528..14906)
                     /locus_tag="IWJ07_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332600.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PDQAKKMSESSDISTVQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRY
                     ISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSAIE
                     DISQSFLLLAFTILELRIYTLMLSGIGDLHAVGKINGFLYVVALIVLTIALFKGSQSV
                     TKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGFGH
                     RKDITHDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGTVE
                     DLTEKAKDTLEEKLDGTRAKEFAEAVKNPSEALEKQKEKASAVKEGLKDAYGEGQVNA
                     AVKANTTSEKENQEGAAIPSAKDHQDELHSNENSIGEKDSEEGQHGQLPKTDPEQYRY
                     KSNINSENEKRISDLPKRNQEVQTDEDSIDLPNDNGIEVGTEKSTKIGLPKTQNLPMK
                     DSRLNQFKENDQSFVHQSPAGTVQMKSSRLPENGNLTTVSYNLAESGETENASVQNIV
                     TKEAAPKPINKVHNGYRPANVAFTAPTKINVTRETKNRNNELKEQLLKDIKP"
     gene            complement(14903..17515)
                     /locus_tag="IWJ07_001006"
     CDS             complement(14903..17515)
                     /locus_tag="IWJ07_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP9332601.1"
                     /translation="MRIRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLKTLKEIQQIYPQ
                     IPESKGSFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     RQSSMVVNDPKTELYVASKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPENEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHKGK
                     KFHEERVKVGYRGFCEINFDCHLEQGDHVCLKYNHQGKQYVAWYEFSQKVLKDEEGKI
                     VYRKKRGETHLPELEKEAVLTLDEKKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSFENETAKKLLIQFYEEQATDRLQEYVEKLIKNEEISEMI
                     RKRLLLEAEKAVKE"
     gene            complement(17515..19546)
                     /locus_tag="IWJ07_001007"
                     /pseudo
     CDS             complement(17515..19546)
                     /locus_tag="IWJ07_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19615..19917)
                     /locus_tag="IWJ07_001008"
     CDS             complement(19615..19917)
                     /locus_tag="IWJ07_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332602.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQGAATSLKNNLMIFAAVAA
                     VASIVLGGIFFFFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGMFGQTPQGL"
     gene            complement(19935..20177)
                     /locus_tag="IWJ07_001009"
     CDS             complement(19935..20177)
                     /locus_tag="IWJ07_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332603.1"
                     /translation="MIASLFIYPEGGIAVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            complement(20174..20629)
                     /locus_tag="IWJ07_001010"
     CDS             complement(20174..20629)
                     /locus_tag="IWJ07_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332604.1"
                     /translation="MKKLIMVFTSLIAMIVILGGCGSEKNKQDSQDNGVLKPGTVWKE
                     EVGGLVHNLKIIDDTTWEYSESVWHPEQVQITVEKLKDYKGLERYKVIDSGGVDLFIG
                     KTALIVPYNKDGLETFKFIPTNEKEHRDKSEILKEYSTKSGYKLQKTSE"
     gene            20817..21008
                     /locus_tag="IWJ07_001011"
     CDS             20817..21008
                     /locus_tag="IWJ07_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332605.1"
                     /translation="MYADKEKIEWLLFETGKTAYEISKHSSVAESTIADLIKKKSAIE
                     KMRFDNAAKLTKYAESISS"
     gene            21153..23210
                     /locus_tag="IWJ07_001012"
     CDS             21153..23210
                     /locus_tag="IWJ07_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAP9332606.1"
                     /translation="MKLLNPKTVIAIGTFAATVALAGSADAASTNVMYRLYNPNNHEH
                     FYTSSTKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVTSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNTLDSAYKGLKKKPAPTVNKTELQALYNQVKSTAKGDFTEGSWNNFQTAL
                     SNAKKVLDDSKASQTQVNNAKNSLDSAFKGLQHKPKPSEYAVTVRHMNRQTGTLLKEE
                     HVSVKAGTSYTAKARTDLTNFEAPQYQVNGNETQTQTINTNTTFTFYYDEVFLVEVKA
                     RPNKVSDTDPNIQELYNYSKSYPVVYGQSITIEAPMFTNFVLDPRESSSNTVTLNNVT
                     SNQGINFAYTHQYNVTVNHINVDTNAVLSTETQTVYEGDSFSTSWKNMTDQNYFLCRN
                     DDSSVTVDKNGQRVINNVDQNRTITFKYKNISLSDLNNYVRQKELAWLNSYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENINSFYLYVD
                     EILSDTGAVNAMVMWKDSAAHNANLLYNNQSIVGLSHAYEVSADGSLSSDNVFISANP
                     IFK"
     gene            complement(23246..25336)
                     /locus_tag="IWJ07_001013"
     CDS             complement(23246..25336)
                     /locus_tag="IWJ07_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP9332607.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLNNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTILAVSLLL
                     LVLATFGYAVLKFN"
     gene            complement(25333..25593)
                     /locus_tag="IWJ07_001014"
     CDS             complement(25333..25593)
                     /locus_tag="IWJ07_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332608.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            complement(25606..26367)
                     /locus_tag="IWJ07_001015"
     CDS             complement(25606..26367)
                     /locus_tag="IWJ07_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332609.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGQKLEKQEGV
                     NGAKFSVYDVSDILQKMDVKDLTTDQIESQLKDRVKKLSSDQLKLISNGETKTIDQQT
                     GVFEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSE
                     ASQPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            complement(26383..27135)
                     /locus_tag="IWJ07_001016"
     CDS             complement(26383..27135)
                     /locus_tag="IWJ07_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9332610.1"
                     /translation="MIIRHPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTIPSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            complement(27148..29124)
                     /locus_tag="IWJ07_001017"
     CDS             complement(27148..29124)
                     /locus_tag="IWJ07_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP9332611.1"
                     /translation="MAINRYVKKAGMIVGMLGIIAGCFQGYRAYAEDVTQKTPPERVN
                     ITVHKLMYDQSTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGELVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGEEHSFQIGDAVNYQGTIHIPTDIAGGTDGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGAISAVVYFKKKA"
     gene            complement(29177..29848)
                     /locus_tag="IWJ07_001018"
     CDS             complement(29177..29848)
                     /locus_tag="IWJ07_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP9332612.1"
                     /translation="MKKWLFGFLGVALIVVCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIKMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAKMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            complement(29930..31096)
                     /locus_tag="IWJ07_001019"
     CDS             complement(29930..31096)
                     /locus_tag="IWJ07_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAP9332613.1"
                     /translation="MSHIQELVEKLINKMSAIFLLLLVSILYLIWTVGLNPPTILFIY
                     DNAAQLVTFFVGCLFLLNLYKVKRNDLFFIAAGILFYLFFSFFRSYRGDTFYGDLIVP
                     TIIAIILIVKWTKFTVIDRSLYLVVFYVFLLVTVYRVFTEIPVPEGQSIWNPSNKLSD
                     IWINTNTIGSSLMTLALLITGFASSFGKWYMRLLSLPALILAGATIWVCQSKAALYAL
                     VIFIILEILPKKVFRNTHLSFWMYAAVSVLALPISYFAAMSDKVHLFTGREEIWRKFY
                     ETLGEKTGQIFVGMKPFFFHRGNQILGNHNTYHALVNHYGLIGFVLSIILLLSFLWLL
                     VRKRELTNGQLTFLWAFLAIMCQSFMEETLTTISWVPIVFLMLAMATHQYKTTR"
     gene            complement(31102..31791)
                     /locus_tag="IWJ07_001020"
     CDS             complement(31102..31791)
                     /locus_tag="IWJ07_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HAP9332614.1"
                     /translation="MNKNNKTLFNPYVGVIIVFLLFSYFAYQGYQKDKIAEKVDGVTK
                     TIHIQEQAKTYQDNLTSLTDISKRQLITNQKQALEKDLKGEIAGKLVFSSTGFILPVF
                     QGANSDTLSLGVASTYYLDTEMGNGNYILAGYNVDTPSVLLSDMTDLSEGEEIDLIDA
                     HYKYQYEVSKRGKISDYSQVVQSKENNSFLSLPKAGKKPLLTLLAFQANNMKKCDVIQ
                     CELVNVEKINK"
     gene            complement(31918..32157)
                     /locus_tag="IWJ07_001021"
     CDS             complement(31918..32157)
                     /locus_tag="IWJ07_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332615.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKMMKQKHLKRLSVMQYINDMKIT
                     GKEKACLLGSMKNFEQLRRTYVKTGSNCQLLLEVS"
     gene            complement(32224..32463)
                     /locus_tag="IWJ07_001022"
     CDS             complement(32224..32463)
                     /locus_tag="IWJ07_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332616.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            complement(32577..32918)
                     /locus_tag="IWJ07_001023"
     CDS             complement(32577..32918)
                     /locus_tag="IWJ07_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP9332617.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQKLTDNPIGFGLLPEDDEGNEWFKMTLTDDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            complement(32905..33234)
                     /locus_tag="IWJ07_001024"
     CDS             complement(32905..33234)
                     /locus_tag="IWJ07_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332618.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTKDLLDFLDKNKDTFIVLTDYEE
                     VTTNEKL"
     gene            complement(33825..34157)
                     /locus_tag="IWJ07_001025"
     CDS             complement(33825..34157)
                     /locus_tag="IWJ07_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP9332619.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            complement(34994..36034)
                     /locus_tag="IWJ07_001026"
     CDS             complement(34994..36034)
                     /locus_tag="IWJ07_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAP9332620.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDSTVIAKIANTAKPRKINDST
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMQEDSINTFVPENVLNLIKTFSSSYSEAQQTVQTIHNAKKKAEELEQTIVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
BASE COUNT        10445 a         7338 c         5587 g        13010 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttgactta
     1201 tcaccattag actaaacaaa tggataatct tctgccgcat cgacgaacgt cttcgtagtc
     1261 agatttaacg ttaaaatatc gccactttga tatgcttttg ccgctttgtc tgtgactaca
     1321 aaagctctgc ctgtttgaat ttctgtcaca aaaacaagat tgtctctgct tttttcaaca
     1381 acaactccaa tcgttgaata agccatttct tcttcctcct ttcttttgat ataatgtaga
     1441 cggaaatcgc accactcaat gaattggcgg aaaacaattc agtgaaaaaa aagagtcctg
     1501 gacaatcacg ttcaggactc ttttacttct tttattcact taacaactaa cattctgtga
     1561 gtgaaataga ctcttcaatg cttttacacg ggaagttatt gatcccctct tcattcattt
     1621 cccaagtagt ataataaggc gctaacttgc cctcatattc ttgaatgata aaatattttt
     1681 cacccttcac atgattacaa cagtacacac cagtagctgg gtactcaacg actaaaaatt
     1741 gatttggatc aaactgttcg tttatcaaca tttctcttat ctgaaacaaa tgctcttttt
     1801 ctaaacaatc gggaagttgt tcgattcgtt cttctcttaa tgatccttct ataactttcc
     1861 attgatctaa atattgtttc attgctattc ctcctactgt ctttactgtc tttactgtct
     1921 ttcttgttcc atttggattc ttcgttgttg ctcttcgtat gctttacttg cgtggtattt
     1981 ttcttcatgg gcattaatcg ttcgatttaa ttgacgcaaa ctccgctgcg tgacaactct
     2041 ttgttggaat tgtttcctgc gcttcatgaa ttcttcgtaa ctatatttgt attcattcgt
     2101 tggcaattgt ttaagcgttc gaaaggatcg ttcattgtaa atagattgcg tctgatcaac
     2161 actttctatt aatggtttta tttgactatc ctgtctcttc agctctgtta ggatggcatt
     2221 tcctgtgcgc ttttttaaat caaagagagc atttttctct gcatcctttg attctctctt
     2281 gctttcccca taaagacttt ttgcttcttc tttcaatgct tttgaaatct gtatatattc
     2341 ttggtattca ggttcttttg ttaacacata ttgggttaac tcgtcgagtt tctctcttgt
     2401 gtatggtgaa agtttagttc gattttcatc cccataattc cattccgaac gtttgtcagg
     2461 taaggtttta tatacttcat ttaataagtt cattgcgtgt cgatcataac gtactggctc
     2521 ttttaccact tttgttaagc tgttacgcaa atcacttttc cgcgttaata aatttcgttt
     2581 ctccatgcct atcattcgat ccttttcata agaaaaacgt tctaattcat gcacataatt
     2641 ggtcttcatt gcgtctaatg ttgcttgtct tctctttcct ttcggtacga tataactcac
     2701 accattttcg acctcagtgt atggtttacg cgtattttct ttttcaacaa ctgcaaaatg
     2761 aatatgaata tgcttagtgt tacggtgaat ggtagccatc caatatcccg tatcattcag
     2821 tttttctttc tcaaacaacg tctccatcat tttccttcca gcagctttta gtccaacttc
     2881 atctaaagta tctgttttgg gatcgtacaa tcctattttt tcaagatacg aattatcatg
     2941 agaaacaaca tcttgataaa tgattgagcc tttttgttgc cctctttgga agatctgctt
     3001 aatctccttc agatcacttc cttttacttc gtctgaagat atagaaaaaa ctccaggaag
     3061 agttcctgaa gtttttttgt gtggttctgc ttgatatttt tccattgctt gattcaaacg
     3121 ttgatattct attttttccc tttccgttag ttcctttttt tgttctaacg catagtgacg
     3181 cgtcatataa gaaagatatt tggtaaaatc ttgttcctct aacttgtcaa aattgattcc
     3241 atattccatc aacgattgcg cttcattcga cgtttcactg aattctttat ccgtcttact
     3301 ctccgaaaaa gtttctcctt caggaatata aaaatcctct aaagcattct tgattcgttg
     3361 cagttcctgt tcttcttcga gcgtgcgccg ttccatttct tccagcgctt tttctcttgt
     3421 catataatca atgtatgttg aaaaagattt tttattgggc aaggtaaatt gacaagtgag
     3481 aatgactgca ggtgatttac tcatcacgtt ctccttctaa tttttatcgt tttaaaaacc
     3541 ttgttggatc aaatacttca ctttttgtag tcactgattc gcttattgtt ttctttatct
     3601 taggagaata tttcttcgtt tgatttgctt ttatcttgcc ttccacacgc tctttcgctt
     3661 cttgataaac agttgaattt gtaccaatta atattcctgt atcttttaaa tgaagatctg
     3721 caaactccga aaccatttca gttaaaatag aaacttcttt tccaattgca tttagtttga
     3781 tttgactggg acttatcttt tcttcagttt tgggatcata catcatacac aaatgtctaa
     3841 ctgcttcact ataattggat atatcgtcat gagtagaaat tatctcatca agtacttcga
     3901 tatgcatatc ataaagataa atatgttttc ttgttgcttt cgccaaagtt gatttcctcc
     3961 ttttataaat tcacaatatt atttttactg ttcaattaac aaggcgatta attgattatt
     4021 tgttgcaata tattcttcta aaattttcgt attataagtt tgaatatcca caaatttttc
     4081 taacaaccga tcaaattgat cttttttata atccagaaga gcgtgatcaa aatcattttc
     4141 gatcaaaact tttaggtatc gatttcggct ccatttagtc ccagtacgtt cacttaattc
     4201 aatacatctt cggtcaattc tttgaatcgt tttttcatca atatctctta tttttacttc
     4261 cataaatatc tccttttgac tatatgttat aacgtgtgta gcaatgtgtg tagctacaca
     4321 cattgctaca catatttaaa ttttactacc cacaaatgaa cttttgtgct acccacattc
     4381 atatttttgc tacccataaa cctacacata aattgaaaat tttggggtca tttatttttt
     4441 tcagaaacgt gataggcaca gcctatctac ccactttatt ttgaaagtgg gtacacgttt
     4501 ccttatgctc tttaaaaaat aaggtgttta aatggaattt tatgttttag attccttgaa
     4561 ttgttcccac tcatcaatca attggctata agtagccatt aatttttctt gcttatgttg
     4621 ctctcgaatc gacaaaaaat cagtttgatt cgttaattct aaatattctg ttcggtattg
     4681 aatgttcatc gtacccgttt taatacatag taactgatta tgatgttctt tcactttgaa
     4741 taactgctcc ttctttaaga tttttaactc cgtgccaact ataagcacaa ttctgtaaag
     4801 aagaatcatt ttcaaaagat tctccttatt tattcatctt tcataggaag ttctaaatca
     4861 ccacttttcc taaaacatat ggtttgtttt gttgaatatt actcccatct aacctcaatt
     4921 tcccaatgcc atatcgtaga cttgcttcaa ctttataaaa attgtagacc tcttttcttc
     4981 ggtcgattgt tttcgtctcc tgtccatctt ttaaagtaga tctgatgccc ttaacggtta
     5041 cttcatggat actcgtgtga acgatatccg tatggatcaa ctcctcatac tttccaaaat
     5101 caacgtaata ctcatagccg ctcagcgtcg gaattagatc aataaccatt tctttttcga
     5161 ttgatctttt aatgagtttt ataaatacac aaccaaacaa agtgagaata aagcctactt
     5221 caaacaaata catatatcca tttataaatt gatcaatcgt cgttaagaag ttctttatcc
     5281 cctctaccgt tttttcagat tgttcgaacc gatctgttag agtcgttaac atactgccta
     5341 agataagtaa gtccaataaa cttttgaaaa gtttactcat tgttaaattt tgcctgataa
     5401 attggacgag taagtaacta aaaatagaaa agcacatgac acacattaat gtcattactg
     5461 ccgtgaaaat atttgacgcc gcgatctgtt ttggctcact taacaagtaa taggccaccc
     5521 catagaccac caccaaataa ccgattaaag taaccataag tgaagccata taaataaaaa
     5581 gttttcctat tggccataat tgcaatgccc aaaattcctt tcgtttgcta tttcttttca
     5641 gaggaagcca tgtgtattca atccagtcat tggattttat ttcgatttct tccttaaatt
     5701 ctttcttctt ttcttgccaa tgagggggca taatttcgtt gatctgtata cataaataaa
     5761 gtaacatgac acacgataac gtactcatta accccttaag taccagatct tcgtcagaga
     5821 acaaaagaaa aagcgttccg acgatcacgg acacactgac gagaaaccca acgataaata
     5881 ggacgatata gctaatccat aaaaactttt gatacgtttt tttcacatag aacccacctt
     5941 aattttttat ttattatcag ctgtaaaata ttttttagca gctatatata gagcttcaaa
     6001 tgatctgaat tttaaggtat ctaaactctt tctaccattc ttcaactcag atattgttgt
     6061 gttagataat tcacttcttg acatatttct ttagctttta caactttgag ataggcttta
     6121 atcaatactt tctgctcagc gaaagtatct ttgccgacca agttcgctcg cttcatttct
     6181 tttactccac ctattcttct aaatattttc ttttgtcata cgatcaattt tttcttgaag
     6241 tttttgaatg ctctcttctt gatctttcat ttgcgttttc gatgtttcaa tttcttgctg
     6301 gtaactatta atcttatttt cagtattttc ctgatcttct gcgacttgat aaggcaattg
     6361 atcttttagt ttcttcacta attcttcatt caaatcaatg gttgcttgga aggattcaat
     6421 ttgtttttgt gctgcttttt gttctttctt gtatcctttc actttgtaat tgatcgcatg
     6481 ttgttcataa tcttcgtctt tcacgtgatc tttgacctta tcttcgtgaa tataaaaaat
     6541 cagatcttgc ggttcttgca tatccacctt tggatcaatt ttttcaggaa taatagttaa
     6601 tcgcatagca atatattctt ctggtacatg tgctaacttc actacaaaat aatcatcaga
     6661 caccttgatc actttggctt ttattgcttg ataatcccct ttagctgtct ttacttctac
     6721 acgataatta agattagcta gttcttttaa taagctctga tcttcatcgt cgatcgcaaa
     6781 ttcaaaaact aattgctggt tcgctccttt ttcttttgaa accatcgtca gttccttcgt
     6841 gtttaaaaat gcgttcgtgc ttacttcatc tggtatgtct acctttttat gtgtgtaaat
     6901 cggactagca agaatcaaaa agccgagaat cataaagatc cccagccatc taagataact
     6961 ttttcgtttt ttaaaacgtt gtcttgctcc ttttttctta ctcattttct tcctcctaac
     7021 gagtgaaata actaaagtct gtgactttcc attgaccatc tacttgtttt tcagtgacag
     7081 ttaatccacc ttttattttt tgtggtttga catctttgaa ttgaggattg ccgattgtat
     7141 aatcaacaaa tacgacaaaa ttttgttcct cttttacact gcttgtttga gcgtaagttt
     7201 gcacgtcacc taatgtaaaa gagtaacttc cgtaatcatc atgttggtga tcatcgtcta
     7261 caggacttaa tttgtcgatc acttcttggg ttgcgacatc ttttagtttt tccgttaatt
     7321 catttctgct taatgattta tcaatggcca attgaccgaa ctcttctgcc gtctttcttt
     7381 gtgcttcagt tacttcctct tttgcttttg tgccatcttg ttgcttcagc tgatcgattt
     7441 cttggttttt ctcattgatg gtttgattta acttgtcaat cttcgcctgt gcttcagaag
     7501 aatttttttt gagcttatca gacgcgtttt tagccacttc ttcaatttta taggtatttg
     7561 tctcttgttc ttctttaagc tgccaaaaca acgcaatatt gccgattaaa ctaacgctaa
     7621 ttgcagctgt taagacgatt tttaaggttt tattttccat attttttctc ctgatctatt
     7681 ttttgctgct attcaaaaat ggtaacggat cgatccatgt accatcatca atgaatgcat
     7741 gtccttgtgc ttgacgccaa tccattcgag taaatcctaa atgcaagtgt gctgtatctc
     7801 gtatcccaat tacttgacca actttcactt gatcgcctac tttcactcga gaatttcctg
     7861 tactatttgc aaattcttga tacaccatat tcaagccatc gtaattaatc acgatcacac
     7921 acgcacccaa acctgaaata ccaggattgc caacgtacac gacttttccg ccatgaaccg
     7981 catgaatctc acttcctgga tgatcgactg aaccaaagtc tagtccatca tggaacccgt
     8041 taggtcggaa ttctcctcct ggatttttcc caaaaagttg tcctccactg aacgaatttt
     8101 tatctaagct acttcctggg aatggattgc cccagcttcc tgtaatcgta ccaccagttc
     8161 cgccgccttc tctccagtgt ttaatcgcaa actcatcgta ctccatcaat ttatttgtat
     8221 taataataga catacaattt ttataatagg cactgtcttc agtaattcct gcggcggcat
     8281 accctcctcg tcctaagatc aataagcttg cgccagggct tttatttccg acacaagcat
     8341 taaaatttga ttgtgtggct accataaagc cgtaatagga gaaggcagct tttgagttgg
     8401 gaaacttcat gtaccagcca ccttcaatgc caccaattcc acgcgcagtt cctttaggaa
     8461 acagacaccc atcaaaccac gtaatcccaa aataattgag atcatttctt gcggacgccg
     8521 acgtaccaaa cgctgattca taaccgtatt gcgaaaggat cacacttggt aataatttat
     8581 acttcactgc tgcacctaca gcatattgcg caatctcatc agaaaaaggg cctcttcctt
     8641 tgatttcttt ataaatgggt aagccttccg aatattctcc tgtaaaagct tcgccaccac
     8701 tacttgaatc agaattatca atagaacctg ccgcaagtat tagaaaaagg gtcacaaaca
     8761 cagtgatcaa tatagtaatc aaacctgcag aagtaccaaa cacccacaaa aataagcgcc
     8821 gctttgcacg acgcttaaca tactgtttga ttaaactttc attttggttt tccattattt
     8881 tcctccatca aacaatttta attgacgttt gttcggacta aaatgaacac gaatggtttc
     8941 ttttgagcca aaattgatca cagcgtctcc ttgattttgt tgaggaatgg attctaattc
     9001 tgagttcgtc attgatttac caactaatcc ttctatatga cgtaaaatag attcatccat
     9061 acgcataaag atcttcgtag aagaaaattc aaagactttc ttcagtttcg agaagttatc
     9121 agaagacata ttttctggca ccatttcttg tggggattgt gtagctaaaa cagtcattgc
     9181 ttctacttta cgcatttctc gttggaagtt tgtgacataa tttaccgttt caatgaaatc
     9241 tggattaatc gcattatggc actcatcaat catcgccaag aagcgtttga agtcttgttc
     9301 ttccatttgt ttttctttaa ttaagcgtcg ttgtcttcgt ccttgaatca atgcctgatt
     9361 ccaaatcaac gtcatggcca tgtacatctg acattgttgt acccctcgat ctaggcctgt
     9421 aatagagtca atgtcaaaaa agaccaactg ttgttggtca aggtgtcgaa tcgtactatg
     9481 accatcaaaa atcatgccat aagtctcaca taaggattcc acgatagact ccatttcttc
     9541 tttccgattc tttttctctt gggaagcccc tggtagttcc agttgtccta aaaaggctaa
     9601 aaattctttg aacgtcggat aagcttctgg atctttccct gtgacttgcg tatcagtatt
     9661 tgaacgcgaa tttgaggaag taaaaccgcg tggtactgtt ccatactctt cataaaaacg
     9721 attcaagtag gtcttgaatt ctgctttcat cgttcctttt agctgaggac tcactaactg
     9781 tacctgatag actactttgt ctaagtgcgt ttgataggac gctgcttcat tgatcttcaa
     9841 atctttgact gaaccatcgt catataaact ggatgcttca tcatacgtcc gtgtggcaaa
     9901 gatctctaaa ggattgatca tcccgtcaga tccgtctaaa cgcaccataa taccgttata
     9961 ttcctttaag aaaggaataa agtcttttcc cttttcaaag ccccaaataa agtctcctgc
    10021 agctaaatgg gcttccgcga tcattttaag aacggtcgat ttaccagctc ccatcattcc
    10081 tagaagcaag gtattggtag ataaacgtac actattttta tacgtggcat tccagacaaa
    10141 tgggccgcct gtcatggttt ccccatattg tgtgcctcct ggatctttta gcgtttggtg
    10201 gtggaacggg aatcctccgc caatgtttaa cgatggaagt gattgccctt tgatatttcc
    10261 aaaccattca gcttgttcat cgaacatggt aaacatcgat tgccaatctc gtttggtttt
    10321 atgcagataa gtcaccgcac gataatccat cccttttaga ttttcgatca gttcctgcgc
    10381 ccgttcatcc acttcttgga tcgtatttcc gtaaacatgt actctcactt tgacttgttt
    10441 agaaatttcc ccattagaag tcaattttcg ggcatattcc gctagttcca ggtattcttc
    10501 cgcactttta tttcggtcag tcgcttttcg ttcattatct gatcgatcat ttaattcatc
    10561 taatgcgcgg ttgatttgat caatcacttt ttgcttttca tctgtgctaa catctagtgt
    10621 ggccactgta tgaggattgt tcatgagatt ggccaaccaa aaaagagtag gattttttgt
    10681 gtaatgataa actgtcacac atcgtgaata accatttcca taggtcacat attcttcatc
    10741 aaaattcaac ccaccttgtg gcatcacttg ttttaagaaa tctaaatcat aaccttttga
    10801 ttttaaaatc tctatttcac gattaccaag ttgttttaat ggatcaatca ccttttgatt
    10861 actttgcgtt gaaaaagctg tttttatcac atctagcatg aatcatttcc tccatctttt
    10921 ttattcgtca ttcatgttgt tcattcgata aagaatctta atcaattctt ttttgtagag
    10981 tggttttaac cctaaaatgc cactagatcg cattaactga cgatttctag tttgtaaatc
    11041 cttgattgtc tttccttcaa taacgaaaaa gaagcttaag tcaggacgtt cttgttctgc
    11101 ccgttttaat tcctggatct cctctactaa tttcttttca tattttctta atcgctgtag
    11161 atttcttcct agtgactgtt ccatacgatt tttatctaat aactgccgct tttgcacgat
    11221 ttgttccgtt agatccgtct ctgtggtcaa actaactaac ttaattgttg gcacatacgt
    11281 acgcatgacg gtggtcaact gattcattcg ttctagtttc tcttgttcat ttaatccctc
    11341 cacgtcagtg gaaacgacac gataataacg ctgaaaatga tcttctctag taattaagac
    11401 tccctcttct gtacaggatt tatatggaaa taagtccaag atagagcgct cttctggttt
    11461 tccaaactgc aaaatggatt tcttatcaac tgaattttgt cctttgactc gcatttattc
    11521 atcctcctta tacgtaccat gataatttcg gttaggatct aacgcatgat agtggttctt
    11581 atccgtaaag aaaatagatt cgataatcgc aaaaatttgg cgttgaccag ggttattata
    11641 gggtcgccaa acaatcacta acgctatcgc aagccataat ccataattca agagtgcaaa
    11701 aaagagtggt aagtggacaa gattattgac tattttcgcc caaaaaaagc agccaccaag
    11761 cgttaataga tctaaaaaga aaaaggcgaa aaacttccat tccccactaa atcttggagg
    11821 atttttcata tgaatgtatt tatccataat ataaccatac cccttatatt ttgtattttt
    11881 agaaataaaa aacactgtga tttctcacag cgtttttcta aatattacaa ctctaattca
    11941 tttcctaaat cacgagaacg attttttcct cgtcgtaatt gataattcaa ttgacgattt
    12001 tcttctttta attcaacaat tttttgttcc agatttcttt tcttttgaat taaatcataa
    12061 ttttgtgcgc gtaattcttc attttccaaa tgaactcgtt gcatttcatc gtttaatcga
    12121 tttacattct ccaatgattc ttgtatcgtt tcttgcctaa aattaagtga gccaagttga
    12181 cgatcttttt gttgtctttg ctctttcaat tctgacgata tttgctcaac agaatgaaat
    12241 atttccgtta attttactgt gaatgtgggc atagcatctt tcaaataagt gaatccttct
    12301 gaaaggtgta caataatgct ttcggtatgt gttttttgac catctaaaat agactgatga
    12361 tctttttgta aactatccgt atctttaaat cctttaatag tcaccacaaa tatcgttaaa
    12421 aatgctccca gtgcagttat aaacgtagct attaaaatat cctccacaat tttctccttt
    12481 ccattttaaa cttgttttat aaagaaaaag gcccttaaat aggcctttta tggtttgata
    12541 tccttcaata gttgttcttt caattcattg ttcctgtttt tagtttctct tgtcacattt
    12601 atttttgtcg gtgcagtaaa tgctacgttt gctggtctgt atccattatg aactttattg
    12661 attggcttag gtgcagcttc ttttgtcaca atattttgaa cggacgcatt ctctgtttca
    12721 ccactttcag ctaaattgta agaaacagtt gtcagatttc cattttcagg caaacgactt
    12781 gatttcattt gaacagtacc tgcaggtgat tgatgaacaa agctttgatc attctctttg
    12841 aattgattca aacgactatc tttcattggt aaattttgtg tcttaggcaa accaattttc
    12901 gttgattttt cagttccaac ttcgatgcca ttatcatttg gcagatcgat ggaatcctca
    12961 tccgtttgta cttcttgatt tcgttttggt aaatctgaaa tgcgtttttc gttttcagaa
    13021 ttaatgttag atttataacg atattgttct ggatcggttt ttggtaactg accatgttgt
    13081 ccttcttctg aatctttttc tccgatcgaa ttttcgttag aatgtaactc atcttggtga
    13141 tctttggctg aaggaatcgc tgcaccttct tgattttctt tttcacttgt tgtatttgcc
    13201 ttcactgctg cattgacttg tccttcacca tacgcatctt ttagtccttc tttgactgct
    13261 gaagcttttt ctttttgttt ttctagtgct tcggatggat tcttcacagc ttcagcaaat
    13321 tctttagctc tcgtgccgtc taatttctct tctaaggtgt ctttggcttt ttctgtaaga
    13381 tcttcaactg ttccttttaa cccacgttca ttgacgtaac caagaccttc tgcggcttta
    13441 gaaacaccat ttttcgcttt cccgaacatt tcggaaactg tcttcttact attaggtaca
    13501 gttgtagctt tatcgtctga ttgttcttcc ccgttatcat gggtaatatc ttttcgatgg
    13561 ccaaatccat tcttacgaat atcatttaaa tggcttaaac cactcttacc agcacttgca
    13621 aggtttttag ctccgctacc cacatttttc gcaatatttc ctaaggcaaa catgctaaga
    13681 agagaattgc tgccgttttt cagactcgta tcaacaccga aaatcttcgt aaccgattga
    13741 gagcctttga atagcgcaat agttagcaca attaaagcaa caacatacaa gaatccattg
    13801 atctttccta ccgcatgcaa atcaccgata cctgaaagca ttaatgtata gattcgtaat
    13861 tctaaaatcg taaacgccaa taacaagaag gattgagaaa tatcttctat cgcacttttc
    13921 atccgttgtc ctgtctcaaa gtccgttgac gcggcaatta ctaaatagaa ttttttaaat
    13981 actagctcaa taatgcaagt aacgattgta aacaaaataa ataaataggc taccgacaaa
    14041 gaagattccc cagccaaaat aatccctgga ctactaatgt atcgacgata gcctgacgta
    14101 tacacatatt ttgctaaacc ttcatcacta atttgaacag ggatttcttt cccattatcg
    14161 tctaactcta gtcgatactg taatgccttt aaccgatctt tttgtactgt actaatatca
    14221 cttgattcag acattttctt agcttgatca ggtgtaataa tcccattgat attcgctgtt
    14281 ttgaaatttt tcgctgttaa agcatttctt ttatctgttg gtaaggattc tactttattc
    14341 gggtcagtcg ctagtaatgt actgacatca atcacattat tttgaataat ttgaaaagca
    14401 ggtgaacccg ttttatctgc tttatttgct gcagtgacgt ctccataaaa gtttttactg
    14461 atatttaacc cttcatcgat taaagaacta ccaccaaaaa tcaagatcat ggccataaat
    14521 aaattaacca agatattttt gactttcggt gggtgtttac tcataaaaaa tttcaagcct
    14581 aaataaatta atgtgacgac acaaaaaaga gccaatacct tactaatcaa actttgaaat
    14641 agttcattaa caccagcggc gttcaataag tcttttaaat caaagctttg ataaattgtt
    14701 ttttctaata aagtattgag attatataac cctttgatca atccccataa aaaagtatta
    14761 caaatccaac tgaatacatc ttgatactgc agataattac cataatcttg taagactttc
    14821 gttacggttt tgggatctgc atccccttct ccgaataaac ctgaccacca gtcggctatg
    14881 ccagagaaaa ttcctccaat gatcattctt tcaccgcctt ttcagcttcc aataacagcc
    14941 gtttcctaat catttcagaa atttcttcat ttttgatcaa cttttcaaca tattcttgaa
    15001 gacgatccgt tgcttgttct tcgtaaaatt gaattaacaa ttttttagcc gtctcattct
    15061 caaaagattg aacgatttta taaaacatat tttcttcgtt tttttcttct aattgatcat
    15121 gcagtccatg atagagattt ctagcatatt catctggtag gaaataatca taatcaattt
    15181 gtaaagcctt aagatccaag tccgcatgtg tacaatgtat gtctatatca ttgatatccc
    15241 acgatggatt gaaatcttca atatattgat aagcataagg aagattcgtt tccccatgat
    15301 taaaaatagg atacggccgt attttatgac ctttcaaatc ttgtcgtttt aaaggagcga
    15361 gtacaatcgt ttcaccttct aaaaaagtag aaattctttc aggagttaag atacgatctg
    15421 aatcaacatt gcgattgata tgagtattca tacctaatga attgatggta ctgttctctt
    15481 gttcaaccgt cttattcccc acttttttag aaaattcttc gatggtatta tcatctgtcg
    15541 acataatata aatatgtgtc tgacaatttt ctttgataat cgctgcgttt tgttctccat
    15601 acttcgcata aagttgctga taagattgaa taaacagatc ccataacata ttacgacctg
    15661 ccgtaaccgt catgattcct tccatgttgt ccatcggtgg catatttcca aattcatcta
    15721 aaataaaatg aatcctacgg tgacatttat ctcctttagt cttaacacat tgcgtagata
    15781 attctttata caactgacta agaaagatgc taacaatgat atggttagat ggatcataat
    15841 caggcgttaa cataaaaata gccattggtt tatcggtata gtgcatccga atagactcta
    15901 ctaataggtt acttttcttc tcatctaatg ttagaacggc ttctttttct agttctggca
    15961 aatgagtttc tcctcgtttt tttcgataaa cgatttttcc ctcttcgtct ttcaatactt
    16021 tttgagaaaa ctcataccaa gcaacatatt gttttccttg atgattgtat tttaagcaaa
    16081 catgatctcc ctgctctaaa tgacaatcaa agttgatttc acaaaaacca cgatagccaa
    16141 ctttgactcg ttcctcgtga aatttctttc ccttatgata aaattccacg acaatgcgtt
    16201 gatttaatag gctttcatgt aatttgagat ccaaatactt cggaaaacca atggacttgt
    16261 aatcaatggt cgattgagaa gtcatcttcg caattttcgt tagggtaaat ggattcaatc
    16321 ctgtaattgc cgtagacaag atattccctt tcgctttatc agatccaatt tttgttgccc
    16381 catattgttt ttttgctata tttccagggg gtaactgatt catgaactca tctaaggcat
    16441 tgagctgctt gaattcattc tcaggatctg gataataatt aaaaccgcca atttctgaaa
    16501 gtagttcaac gagattattt ggtgttaatt tggagtaatc ccctgtttga tccgcttgtt
    16561 ccaataacgc aaaaataagc gcattgacag ctttctgcgc attttcgtta aagtatttat
    16621 tgtctgaagc atttgcatca taataaatcg tattcgtaaa agtattggtt aactgtgttg
    16681 cggtgtctat gtctcctcgc ttccagtatt tcacaatgag tgccaatgga ttatcactca
    16741 ttgattgcag tggatcggtg atattaaagg catacacatc ataccctcta gcttctagtt
    16801 catctttact agcaacatat aattctgttt ttggatcatt aacaaccatt gaactttgtc
    16861 tttctgcacg cgacaaattg tcaatggttg ctaaaacttc tatctggcct ttccctgagc
    16921 gggaagtacc aacaatacaa gtatttactg tatccgtctg aatataccag tattttttat
    16981 aatgagtaat tggtattccg ccataaccag gaaaacttcc ctttgattct ggtatttgcg
    17041 gataaatttg ttggatttcc tttaacgttt ttaatcggtt atctcctttt tgcccatctg
    17101 ctagcggacg aaaccacatg tccattttca atcccataag tacagctgga acaacacaaa
    17161 tcgtagaaac aactaaacgt gtgcctaaac ttgttgttgg ggtcaaagga ttccaaaaac
    17221 cagttgaatc aaaatggaat aactgcggca tttcttgtaa ctgaaaaata ttcactcctt
    17281 gtaaacttcc tggaataatc tcaaatcgat gaaatgcttc tggtattctt gtaacaacat
    17341 agtttaaagg aagataagct aagccactat aaaaaatctt ttgagaaata cgtttgtcat
    17401 gtttttctgg tagctcttct cctgaaggag tatagcgata gaaatgagaa agatacctat
    17461 tcttttctaa attgaatcgt ttgtcttttc gtctcgttgt ttctctaata cgcattattt
    17521 cacctcattt gattctgaac tttcggaagt gtttgagtgt acagttgaat cgcttgatcc
    17581 ttctttagcc ttactaggat cactctcttt gctattttcc atacttgact ctgctgttga
    17641 ctgattggta cgattcaata attcttccaa ttgttgttca tactcttttc ttgcttgttc
    17701 ctgggcttct tccttgattg acgccgttgt ggctacagaa tctgcctgtg aagattgatt
    17761 gatatgatta aaagcaaata ctgttcctcc acctagaagc aagcttaaac ttgcaataca
    17821 tcccaccatc catccgttaa aggaacgctg tggtgaagga gttaaatttg cttggttgtt
    17881 cactggcgtt tgaacagcag ctactggttg aggttgtgct agataattca actgtgcttg
    17941 tcgcaatgta tcgagcagct gcttcttttt ccttttcttg ctctaaccgt tgtttttcta
    18001 tcgccacttt ttgttctgca agatgctgct cacgtttttt tagttctaat tgttcgcgct
    18061 ctttttcttc ttgtcgttgt ttttcttctt gttctttcaa ttcttgttgt tgaatttcat
    18121 actctttttt ccattttgga atttgtgctt gtaattcttg ctggatctgc ccgtgtcttt
    18181 ccataagaac ttcagtcgta gtttgataga gtttattcag ttgttcattt aattctttgc
    18241 taatctttag cttatctgta cgtaaacctc ggtagaactc ttcttttcgt tcttttatac
    18301 gttcttttaa caagttttct ttttctttcg ataaagcttc ttgattagtt ttagtaaagt
    18361 tattaatttt ctctatacac aaggaatacg cattgtcgcg tttcttttcg tatcgctctt
    18421 taattttttg tacttcttct tcaatcactc gttgttcttc tttatttagt tcctcttttg
    18481 ttctctccca attagatgac tgttccgtcg catactcatt tatttcttga tctgtttctt
    18541 gttgacgttc ttcaaaagtg gctaataaat cttcttgtgc aagctcatgc caatttgttg
    18601 attgaacaga ctctaactgt tctaaaagtg tgcctttacc ctgtgagatt aattcttctt
    18661 tactctctaa aaatgcttga taggtttggg gtaattgata tttcgaataa agctcattta
    18721 aaaataattt atttttttct ttcatccatt catgctctgg cggatattga tccaaaatat
    18781 aatcaaatac agttgccttt tgtatctctg tttcgttttt ctctacttct tcactgataa
    18841 gtggttcaat atattcctga gaatcccctt cagtccatgt tggctcttca ttagcttcaa
    18901 ctagatcaac ttctacttcg gccgtctctt catcctccat tgtttcttca aagggatcta
    18961 cctccaattg acccattaca tcgttctctt cctcaaatat cgatttctga gtcggaatat
    19021 cagcttgttc gtcaacatag actgtctctt tttcttttga ctccttcttt ggaaagattg
    19081 gtcgttccca tggtacttca tcatttactt cgacttcact cacttgactc acttttcttt
    19141 ggtcgctctc taactcttct tctaaaactt gtagcagttt ttcgtttcga taagactctt
    19201 ccacactcat ggtttcatcc gtttccttaa acacatcaag gcggatcaaa tccatcaatg
    19261 taggataaaa accttctgaa atttgataat ccagtgggcc aaaaacgtat tctgtgccag
    19321 ccttttctat ttttccttct ttatcatgta acgtgagagt caatttaaca gaagattgat
    19381 tctcttgttc taacacttgt cctaacacat gtagctgttc ttccgtttca atgacagaat
    19441 atttaattgt tttcggtaaa aaggcagtac gctttcccac tttcttcgtt gcttttttta
    19501 ttaactcttt atcttttttg gtattgcctg aaatcgtaaa gtacatgagt attctccttt
    19561 cataaaaaag gagtaaaaag tagccacgta ctttttactc cttctattta ttttttatag
    19621 tccctgtggg gtttgtccaa acattccaaa taacgaaacc acaatagagg cggcagctaa
    19681 tataactcct acccccatca atacataacc tacgtgcatc ttcccgtttt cagctcctct
    19741 tcggccaaag aagaagaaaa tccccccaag aacaatggag gcaacggcag ctacagccgc
    19801 aaaaatcatc agattgtttt ttaaactagt tgctgcacct tgtaatgctc taaaaatatc
    19861 aaccgcagct actactttcg gttgtgttaa aaaattcatt gtaattgatt caaacataaa
    19921 attgttcgct ccttttagtg tttaataatt tcatcgaaaa caagcttttc acgttcacgt
    19981 tgatagatat atccttctaa gtccttgtct tttccccgta ataggtcaaa ttgtgctttt
    20041 tcaaggactt cttgtttcca ctgtgtttca gatacaccgc gacgttctaa gattttagac
    20101 gcggcttttt caaatggcgt tttagctttt gccactgcga ttcctccttc aggataaata
    20161 aaaagactag caatcactcg ctagtctttt gtaacttgta tccagattta gtactgtatt
    20221 cttttaatat ttcactttta tctcgatgtt ctttttcgtt tgttggaata aatttaaacg
    20281 tttctaaacc gtctttgtta tagggaacta ttaaggctgt tttacctatg aataaatcta
    20341 ctccacctga atcgattacc ttatatcttt ctaatccttt atagtctttt agcttctcaa
    20401 ctgttatttg tacttgttca ggatgccaaa cactttcgga atattcccat gttgtatcat
    20461 ctataatttt taaattatga acaagtccgc ctacttcttc tttccacact gttcctggtt
    20521 ttaacacgcc attatcttga ctatcttgct tatttttttc actaccacat ccacctaaaa
    20581 taacaatcat ggcaatcaac gaagtaaata ccattattaa ctttttcatt ctattccttc
    20641 tttcgttgtt ttattttagt atattaaata acgaaagtag caacaaacct cattttgata
    20701 cacggggaac ggatactcat aatttcccac ctttctatag aaacttttcc tcttgatatt
    20761 gtgatttatt acaaaaaaga gctataataa agaaaaaatc aagagggagc attgcaatgt
    20821 acgctgataa agagaaaatc gaatggttac tatttgaaac tggcaagaca gcatatgaaa
    20881 ttagcaaaca ttcttcagtc gcagaatcaa caattgcgga tttaataaag aaaaaaagtg
    20941 cgattgaaaa aatgcgattt gataatgctg ccaaactaac caaatacgcc gaatccattt
    21001 cttcatagca tataaacagc catccttaca ctcttcatag ggcggctttt ttatttcaaa
    21061 atttccacgg tataccgttg actacctcgg tataccgtgg tatattatga atgtaaacaa
    21121 aatatttaat catttaatag ggagttgaaa acatgaaatt attaaaccca aaaactgtta
    21181 tagctattgg aacatttgct gccacagttg ctcttgctgg atctgctgac gctgcttcaa
    21241 ctaacgtcat gtatcgcctt tataatccaa ataaccatga acatttttac acctctagca
    21301 caaaagaacg tgatcaccta gtgaaaatta agtggggtaa ttatgaaggc cccgcttggg
    21361 aagcaccgac tagcggtggc caccttgtct atcgtttata caataaaggc cttcgtgatc
    21421 accattacac agctagttgg gatgaagtga aatggttgac caaaaattac ggttggactt
    21481 atgaagggcc tgcctggcgt tctgccgaaa aaaataataa accgatctac cgccttttcc
    21541 ttccaggtgt tacctctggt tcgcatcact acactgcaag ttggaaagaa gtacaatggt
    21601 taaccaaaga ctatggctgg aaatacgaag gtattggttg gtatggtgct gacacctcta
    21661 aacccaaagt gaataaatca gctttagaag ctctttatac gagcgttaag ggaactaaaa
    21721 aaggtgacta taccgacaat acctggaacg cattccaaac cgctttaaac aatgctaaaa
    21781 aagtgttaaa tgactcaaaa gcgactcaaa aacaagtgga tagtgccaaa aacacattag
    21841 attctgctta caagggatta aagaaaaaac cagctcctac tgtgaacaaa acggaattac
    21901 aagccttgta caaccaagta aaatcaactg ctaaaggaga ttttacagaa ggatcttgga
    21961 ataattttca gactgcttta tccaatgcta aaaaagtact agacgattct aaagcttctc
    22021 aaacccaagt aaataatgct aaaaatagtc ttgattctgc atttaaaggg ttacaacaca
    22081 aacctaaacc ttcagaatat gcggtaactg tccgtcatat gaatcgacaa actggaacgc
    22141 tgctaaaaga agaacatgtc tcagtaaaag ctggtacaag ttacactgca aaagctagaa
    22201 ccgacttgac taactttgaa gcaccacagt atcaagtaaa tgggaatgaa acacaaacac
    22261 aaacgattaa cacaaatacc acatttactt tttactatga tgaagtcttt ctagttgaag
    22321 tgaaagctcg tccaaataaa gtctcggata ctgatccaaa tatccaagaa ctctataatt
    22381 acagtaagag ttatcccgtc gtttatggac aaagcatcac aattgaagca cctatgttca
    22441 ctaattttgt tcttgatcct agagaaagct ctagcaacac tgtgacttta aacaacgtta
    22501 caagtaatca aggaatcaat tttgcctata ctcatcaata caacgtcact gtgaaccata
    22561 taaacgttga cacaaacgct gttttatcta ctgaaacaca aacggtttac gagggagata
    22621 gtttctctac ttcttggaaa aatatgaccg atcaaaacta cttcttgtgt agaaatgacg
    22681 attcatctgt taccgttgat aaaaatggac aacgtgtaat caataatgtt gaccaaaatc
    22741 gaacgatcac tttcaaatac aaaaatatct cactaagtga tttaaacaac tatgtgcgtc
    22801 aaaaagaact tgcttggtta aatagctacc gtcaacaaaa tggcgttgcc ccaatgcaat
    22861 tcaatgatat cgttcaacaa gctgcagata ttcgtgcaaa agaattacaa gtaagcttca
    22921 gtcattaccg acctggcggc ggtactttcc aagatctctt agaaagctta ggctgctacg
    22981 gtgctaaagg agaaaatatc aatagtttct acctttacgt tgacgagatc ctttcagata
    23041 caggagcagt aaatgctatg gttatgtgga aagatagcgc tgctcataac gcaaacttgc
    23101 tttacaacaa tcaaagtatc gttggtttat cccatgctta tgaagtttct gcagacggat
    23161 cactttcttc agacaacgta tttattagtg ccaatcccat tttcaaatag aaaaacaagg
    23221 ctgattttca gccttgtttt tttgtttaat tgaacttcag aactgcgtag ccaaaagttg
    23281 ctaacacaag taatagtaag ctgaccgcta aaatcgttac taaatgtgta gaacctgttt
    23341 taggaacaat aacttgtggc aagttaggat caaccccaat tgtcacaaca tttgttttat
    23401 tagaaacatt atctactgct actacggctt ggttatcaat atttttttct gattgtaatg
    23461 gagttttagc tgtaaatatc agctgaattt ctcgtccatc taaaagattg ttatttaaga
    23521 agtcagcgga agcttcccac ttcagcgttc ttgaattgct atcataggtt agtgtgcctt
    23581 cagaagtaat atcttttcct ttactcttat ctattacttg cgcagaacct tctttatatg
    23641 tcaaacgagc ttcaattgga tcttgaatac taaattgttt gtagtgatct gacgcagcgt
    23701 ctacgccttt tgtgtgccat ttttgtgtga ctagatattg tacttcagat cctgcgttta
    23761 aaggagctgt cggcgtaccc ttcttaccgt catttcgtcc gtttacatct tttccatttt
    23821 gttgtacttc tttcactgga attttaggga aataaacggt aatgatattt gtatcctttt
    23881 ctactttatt aatgatcgac gttccctgat ttttggctac actattttga ttggccaatt
    23941 cattgacttt tacattgatc actaattcgt aagtttcgcc gttatacttc attcgatttt
    24001 ttaatgtatc agctttcgct gcatatttta cctggtgttt tgttttgtca taggtcactt
    24061 caccagatac atcgaatttt ttatttgtac cactttcaac gtgtgcatcg acgtaattta
    24121 cttcttttgg taaattatcg attaactcaa atcgatcgta aaccgctagt aaatccactc
    24181 ctaatcgatt caccttctgt ttgattcgat attgaatcgt gtctcctact tgcaacattt
    24241 tatcgttcac gttatttcca tttttatcaa cccctgtttt ttcaggttga tcaggaacaa
    24301 cactaaagat tgtcgcagaa gataaagtaa accaggtttg aacattagaa gaaccaatca
    24361 caaacgtcgg atttgcttca gatattttaa aggacacagt accttgacca aacttcggat
    24421 ctcctaattt atcatgcaaa ttattgaaat cattatccgc ttgtgtcagc ttagagcttc
    24481 ctcctgggtg gccacctacc acgttcagat tgttgtagaa agatttaaat tctgaaagaa
    24541 ccgtatcttt tctgacatac caatctgtcg tattcatttt gtcatagtgg gcatattctc
    24601 ctttaagatc cgtgcttttt cctcctgcag ggttcaaaga gttaaaactg ataaatgtat
    24661 ctcctgaaaa ttgaacgggg ctaccgtcct catatgtggc tactagctgc acattcattt
    24721 gtgttgaatg gtaaacaatt cctgagaaaa gattgtctga gatccaaagt actgttcttt
    24781 ttttagattt actcattgca ccattcgtat ttttatagcc atcgtctcga aaatgagttt
    24841 ttgtatagtt gttatctagt agattccacg gcaacgtatc cgagtataag tgaatattag
    24901 atagaactag cttgatattg acttttttcc cgttatacgt accgattttt ccatagtcaa
    24961 tattgacttt ggtgttggtt gttacgcctt ctaaaacaag gtttgtttca ccgtattctt
    25021 tttggaaaga aggatagcgt atatttttgt ttccctccac taatcctacg tagtcgtctg
    25081 taaaaccacc tttgttggca tcaacatgat atttgtaaac ttttgacgct ccgtttacag
    25141 taatatttgt ttttgcatta ggaacatctt ttggaataat aaaattattc tttacgttct
    25201 ggattggatt tgacggatcg acaataactt tttcagcaaa gacattggct gtttttaacc
    25261 caaacaatgt acctaacatc atcattatgg gaaaaacata ttttaaccgt cttgtccacg
    25321 agtgtttaaa attcatccct tcatcccctc ttttttataa atgactagtg aaattccgac
    25381 aataacgatc aaattgatcg tagtaaataa tggattccta atgagctttt ccataatatc
    25441 tagcggcatg gaagcagctg gtatgtttaa aatcctcatt aggtatagtt ggtagcttgg
    25501 caaaatagat agtttagaaa ataataaacc gcagtacaaa aagactgcgg ttaaacaaga
    25561 aaaattgaat aataataatt tgatcagttt cattttattt cttccttaat ctctagaaaa
    25621 caagtgagta acaaaatccc aacaattctc aaagaagttt ttctttacgc cagtagatac
    25681 gatttttttt acttcttctt taggttgcga agcttctgat ttaggataaa tccacacatc
    25741 ctttaagaat tcaccatttt tatcagaaac aggtgttaat agcaaaatat cctcgctatt
    25801 agaaatgttt tcaggacttg attcattgac aatataataa gcttgtttct gatttgcctg
    25861 tacttctacc gagaattcaa agacacctgt ttgttgatca attgtctttg tttctccatt
    25921 agaaattagc ttcagttgat cactggatag ttttttcact cgatctttta attgggattc
    25981 aatttggtca gtagtgagat ccttaacatc catcttttgt aaaatgtccg atacatcata
    26041 aacagaaaac ttcgcgccat ttactccttc ttgtttttca agcttttgcc cctggttgtc
    26101 taattgttcg gaagaaataa ttttttgaac gtgaatggtc tgtttgacta tttgatcgtc
    26161 ttcagcaatt ttaggttctt ctgaagaatc aactgttgac gaatcgacgg tttcatcgct
    26221 gctttgaatc gtgctgctat ctgacgtttg gattgtttgt tctgtgctat ccgtgctttc
    26281 ttgtctgtct gcaaacgtca actgtgtgta ggcagtgaaa gaaaaaagag cggctgcaca
    26341 aacaaatagc cattttctat ttctcatgtt ggttaccctc ctttattttt tctttttata
    26401 aacaaatagc gaaccaatga tcatgactga aagaaaaaca gtacttccta aaagccattt
    26461 ccaagataat ttttgtttct tattttcttc atcttttaat tctgattttg taaaggttac
    26521 tcgcttccca tacactagga aacggtgtgt gttgatcccc ttaggcgtac atgtcattag
    26581 cgttactcga tcttcattgg gatttggttc taagtaagtg acaaagtttc tttgtagttc
    26641 gtctttttgc acttcctcaa tccgatcgat ttgataagcg tgctgctcac catcaacttt
    26701 gatataaaac ttttctccct ttttaactga aggcaaatca tcgaaaagat tatctttata
    26761 aagtccactg tgccctgcga ttaaaggatt tttaccgttt cctcctgtaa tatcgccagt
    26821 tccttccgta atccctacac cattctctaa aattttgtcg gaagttccgt cataaattgg
    26881 tagttttaaa gaaatacttg gaattgtaag aataccaaca ggatcgccta actcaatctt
    26941 agcaacatcc aaagaagaat ccgtttctgc tgccgttcct gttcgatcga ttgcggcaat
    27001 ctgctgattt tgttggtatt tcgccacgct cgcttcagca ggcatcaaaa gtaagacacc
    27061 aacagctgaa agtaaccaga aagcaatgat ccatttaccc atgattcgct ttttcttagg
    27121 gtgtctgata atcattagct gatccttcta ggctttcttc ttaaagtaaa caactgcaga
    27181 aattgcgcct gccccaagca acaagcaaga gatcaatacg taagattgga aaccgttgct
    27241 accagtcatt ggtacggaag ctttttgatt atttttgatc gtgattcggt tttcttcttt
    27301 gtatgaatct ttgtctactt tgaaagagat ctcttttgtt aatttttgat agttttcagg
    27361 agcttttgtt tcttccagtg agtaatctcc ttcagttaaa ccagtgattt caaaatgacc
    27421 ctctttatct gattttagga ttgctgcatt tgctacatcg tctacccaaa ctgcttcttt
    27481 gacaccatct ttatctgtat cagtaagtcc accgtagtac ttaccttctg cattcttagc
    27541 tgcaaattct gcgccttcaa gcggtgttgc tttgatacct gttccaaata ggccgctatc
    27601 gacttttaag aattttgcgc cataagtcac aacgtcagct ttcgtttttt cgtggtgttc
    27661 ttcctgatta aatggattat tgttataaac gaaatccacg ctatttgtta atggatttgc
    27721 gatttcagca ctatcattca caaacatgtt ataagtcatt tttagatctt gaccatgtaa
    27781 attcgcaact gtatcactta cttgtccatt atttacgata aaatcgatca caaaaccact
    27841 atcagaattt tcgattttat agtcagtatt ttcttttaat acgactgaac catcattatt
    27901 tgttacttta atatctgctt tttcagcaac ataagaaagt cctttacctg ctgtgtcgcc
    27961 ccatttaaat acactgtaag gagaagtttc actttttaca ccatttactg taatcccatc
    28021 tgttccacct gcgatatctg tagggatatg aattgtacct tgataattca ctgcgtcacc
    28081 aatttggaaa gaatgttctt ctcctgttcc atttgtcact gttttatcga tcactggtgc
    28141 tttatagttc acgtaggaag cttttaaatc acttgtcacg ccgtcattcg taatttcaaa
    28201 ggttaactta ttgtgcgcat cgttgcgagc gtctgcgcct aacaaatatt ggtctgctgt
    28261 tggttcttga tctgatccca ctacaacttc tgaaggaact tccacaaagt agtattttcc
    28321 taacgtcaaa tcagttgcag tcagctttcc ttgatcatcc gatacaagct ctcctaattt
    28381 tgttcctttt ccaggttcgc ccttgtataa ctcaaatttt gcccctttta aaggtgtttg
    28441 tttgctttga gctgtgccat cgtcaccaaa tttcgttaat tcaaaactca acttagaaac
    28501 aatgttcttt ggatacaaat gaatatcttt taaaaatcct gatcctgtat tgtctgtcat
    28561 tggcgcaatc acaaccattg gttttgcttt ttgtgtgact aatccagcag cggatttact
    28621 ttcataaacc acatatacat tccctttgga attgacatag gctggttgat cagcaaaagt
    28681 gatctcacca ttttcatcta cagctgaagt aattttattg gttgcattct taacgtactc
    28741 actgttagat cccttgtctt ctacatcttt aacgatttcg tccactttcg cgttggttag
    28801 atcagaatct tcagtcggta atacttgatc cgtaatattt cctaacgtaa actcaacatc
    28861 accataatct gccttgttgt atttcgttac acctgtagga tattgatcgt gcgtataacc
    28921 atcatttttg attccgtcaa tatcaacgtt caattgcgta ctttgatcgt acattaattt
    28981 atgaaccgtg atattgaccc tttctggtgg cgttttttgc gtgacatctt ctgcataagc
    29041 tcgatacccc tgaaagcagc ctgcaatgat tcctaacatt ccaacaatca ttccagcttt
    29101 ttttacatac cgatttattg ccatttttta ctctcctcat ttcatatttc aacgactcgc
    29161 gttgattaaa ttcttgttat tgtagctctc cctgtaccac gtatctttct tttccttgtg
    29221 acgtataaac acaagtaaac aacgttaata agggtttctc tcctttcttt ggtaaagaca
    29281 agaaagagtt ctcttcagga acgccatcga ttaatttaaa gtactcaggt acgataaatt
    29341 tacgcgttac cttgtaacga tagacgccat cgttactaac taagtccatc acctccccta
    29401 atgacaactt ttgaatatca gaaaataaaa cacctggcat ttccatgtta tgacctgcta
    29461 ggacatagtt tcctttgccc attttggcat cttcataaaa ataagtagcc gctccaagac
    29521 tcaaagtgta ttgatttgcc cctttaaaaa ttggcaatgc taaaccaagt gcaggaatct
    29581 gaatttgtgc aaatacttgt ttattgacgc ccattttgat tgcttctttt tgtatggaca
    29641 acatttgccc caaagacata tcattattga cgttcgttgc atcctcctta taactctttg
    29701 cgtgtgcatt gatttgatct acgggcatct ttttttctat attttgctta aacacctctc
    29761 cttcatgttt ttggtagcta acatagccaa acacactgca aacgacgatt aaagcgactc
    29821 ctaaaaaacc aaataaccat tttttcatcc atctgcctcc tttcagacaa aacaaaaaac
    29881 actttttcac aacgaaaaag tgttttctct tattactatt ctatttcatt tatctggtgg
    29941 ttttatattg gtgtgtagcc attgctaaca ttaagaatac aattggcacc caactgattg
    30001 tcgttaatgt ttcttccatg aacgattgac acatgatcgc taaaaaagcc cacaagaatg
    30061 ttagttgtcc atttgtcagc tcacgttttc ttacgagcaa ccataagaaa ctaagcaaaa
    30121 gaataatcga tagaacaaaa ccaatcaatc catagtgatt gacgagtgcg tgatacgtat
    30181 tatgattacc tagaatctga tttcctctat ggaaaaagaa aggtttcatc cctacaaata
    30241 tttgtcctgt tttttcgcct aatgtttcat agaatttgcg ccaaatttct tctcgtcctg
    30301 taaataagtg aactttgtct gacattgcag caaaataaga aatcggtaaa gctagaaccg
    30361 aaactgctgc atacatccaa aaggacaagt gtgtatttcg aaaaaccttc ttaggtaaaa
    30421 tttctaagat aatgaagata acaagcgcat ataatgctgc tttcgattgg caaacccaaa
    30481 tcgttgcccc agcaagaatc aacgctggta atgatagcaa tcgcatgtac cattttccaa
    30541 atgaactagc aaatcctgtg ataagaaggg ctaatgtcat taaagaacta cctatcgtat
    30601 ttgtattgat ccaaatatct gataatttgt tcgatggatt ccaaatactt tgtccttctg
    30661 gtactggtat ctcagtaaat actcgataaa ccgttacgag taaaaagaca tagaatacta
    30721 caagatacaa gctacgatca attacagtaa atttcgtcca ttttacaatc aaaataatag
    30781 caataattgt cggaacgatc agatcaccat agaaagtatc tcctcggtag cttctaaaaa
    30841 aagaaaagaa aaggtaaaaa agaatgcctg ctgcaatgaa gaatagatcg tttctcttta
    30901 ccttgtacag attcaaaaga aacaaacagc ctacgaaaaa cgtcactaac tgtgcagcat
    30961 tatcataaat aaataatatc gtaggaggat ttaaccccac cgtccatatt aaatatagaa
    31021 tggataccaa cagtaacaga aaaatggcac tcattttatt gatcaatttt tctacaagtt
    31081 cttgaatgtg actcacttcc cttatttatt gattttttct acattgacta attcacactg
    31141 gatcacatcg cactttttca tattatttgc ttgaaaggct aacagtgtga gtagtggttt
    31201 tttacctgct tttggcaagg ataaaaagga attgttttcc ttagattgaa ctacctggct
    31261 gtaatcagaa atttttcctc ttttggagac ttcatactga tacttgtaat gagcatcgat
    31321 tagatcaatt tcttctcctt cagataaatc agtcatatcg gataataaaa cgcttggtgt
    31381 atctacgtta taaccagcaa gaatataatt tccatttccc atttcagtat ctagataata
    31441 agtagaagct actcctaaac taagcgtgtc gctattcgct ccttgaaaaa cagggagaat
    31501 aaatcctgta gaagaaaaaa caagttttcc agcaatttca cctttgagat ccttttctaa
    31561 tgcctgtttt tgattagtaa tcagttgtct cttagaaata tccgttaaac tggttaaatt
    31621 atcttgatag gttttagcct gttcctgaat atggattgtt tttgttacac catctacttt
    31681 ttcagcgatt ttgtcctttt gataaccttg ataagcaaaa taactaaaaa gaagaaaaac
    31741 aatgattacg cccacgtaag gattaaataa agttttattg tttttattca tctatctacc
    31801 ttctatatat gaattaattt acctacatct aaccaagata aaccatgtta aattttacta
    31861 ttctttttat tcaataacaa tgccacatca taattgatca ttttcatatc ataatcgtca
    31921 agaaacttct aataatagtt ggcaattgga acctgttttt acataagttc gcctcaactg
    31981 ttcaaagttt ttcattgaac ctaacaaaca agctttctct tttcctgtta tcttcatatc
    32041 attgatatat tgcatgacag ataatctttt aaggtgtttt tgtttcatca tttttagcaa
    32101 cttttcttcc atttctttcg acactttttt tctgttttcg atttcttcat aattcatgag
    32161 ttttcctacc tttcttgttt tttgcaaaaa taaaaaagcc tatgatcaca tacgctctac
    32221 acttcagtct tgggttatct ttttctttcg tttaattact ggccgtgctg gtttcaaatc
    32281 cttgattcca ttatgggttc tccaataatc gaattctttt cgtaatcttt ctttttccat
    32341 atctgttcct ttagacaggg atttgaatac atctttaggt gaataaccac gagctaacag
    32401 ctgtacatac cttccttttg tcaaagtatc catatccatc gcctctaaaa ctggtcgcgc
    32461 catctaattt tcctcctaaa taaattcctt actcaatgcc taaggtaata ctaccttttt
    32521 attattcgtc gtgactcctt ctaagccttt atctgtaaaa tttattcctc cagtttttat
    32581 tcttctttat ccgctacaaa ttcaatgatt tcaacgctga caatataatc actcaaatat
    32641 gaccaagtat cttcaacaga taattcgtct ccattatcat ccgtaagcgt cattttaaac
    32701 cattcattac cttcatcatc ttcaggtagc aagccgaatc caattggatt atcagtcaat
    32761 ttttgatcgt ataaatcatc ctcgtctaat gctaaaacat ctaatccttt acaatttccg
    32821 cctcttctaa acgtcacaaa tcctttgtaa tcccaacgtt ggaaagaaac cttaatcgta
    32881 tgtgtgccca atcgtatatt cggatcataa tttttcattt gttgtaacct cctcataatc
    32941 agttagtact ataaaggtat ctttgttttt atccaaaaaa tctaataaat cttttgtaga
    33001 agatatctct tcatcaactt caccataata aataccttcg ataattgttg ctatatctga
    33061 aaaactaatt tttactggac atttatcgtc taccgcattc aataccgaca aaattgcttc
    33121 tttttctgca atctctcgtt tgaatttctt tggagctaat cgcatgacac tcaagtcatt
    33181 ttcaagcttt tcaatccatg cagttttact cattttcgca aatttttgaa tcattcctct
    33241 accacctttg ctatctacta aattagtttt acttcataac gaatatcttt atcgtttttc
    33301 gttcactcaa aaatatataa aataaatcga ctcgtttatg gcgtccgaaa cgagaaaccc
    33361 tagtagacag cggtaaatgc tcgattgatc caatgatcaa cgtcctcgca agtatctaca
    33421 tgtactgcag cacacgacac aatttcttgt gcgtcctccc cattacgaga agacctactc
    33481 cttattccat gagtttgcgt caaagcctag atcccttagt agctgctcat gattaatcta
    33541 ggcgctattc taacttgtct aacttctaaa ttccctcttg tttactctgt gcatattatt
    33601 tacacaaatt catttcacca ctatccaaac aagggcattt actcgattga ccaatttttt
    33661 acgttaccaa tcgtaaaaac ctaccccgtc actccccgaa gggaattgtt gatagatcaa
    33721 gtctactcct tatttttcta gtgattctaa gttcctttag aagtctcgac aatattttca
    33781 agcaagcgaa aaacgataaa atacaagtta tgagaatgat caattcagct gattaaacgc
    33841 cccatccctt ttgcggtaca acgcaagact ccattttttc ttcgaccaat cagtacaccg
    33901 ttttggtcat aaacatgttt gattgttgga tatagctctt ttaaagggat aggatctttt
    33961 aaaattattt gtttttgttt cccaatgtaa taaatcattt cttctgagac tttgtctttc
    34021 tggattatat atttcttaca ctgcacaacg gttttacttt gaccagttga tagtttcttc
    34081 gtgatctttt ctgtagcggt acaccgttta ccgtagtgac aaatatcttc gagactcaaa
    34141 attcctgtat atgacataaa ataatccacc cacttacttt aaatattttt aacaagtttt
    34201 tcttgttatt tttcaaaaat agagaattga ttttcgttca tgacaaacaa aaaagaggga
    34261 acgcattcaa acatgcgttc cctctaattt cagcgttcgt catacggcaa agagccccac
    34321 ggacattttt ataacattat gcttaggcat agattcatcg taatatcacg tatttttaac
    34381 cttaacacag ccaaaaaaag tggtatcact tttgaatatc tgttatacta aatccgttac
    34441 gggtaatacc gtaacactga aaggtaaacg cattaagggg tatagactta atgccacctg
    34501 tggacgtcga cgaaggttac cagccttccg agtctcacag ggcctttctt ttttttgtat
    34561 tttcaattgt ccaaatttat cgtccaaaag tgcagaacga aaatcaattc atcacaagca
    34621 tagcagacaa ttaaatttaa aacaactaaa aatatacaaa aaagtgtaat tgaatattag
    34681 cagcactgcc tgaaaagaag taatatcagg cttttcagct tggggaaata agtgaattac
    34741 ctctcttcat gaattattat aactcaaaaa agcacatatg caaacactag ttccctattt
    34801 ttttgagtga aaaataacta aaatatctaa cgattatgaa tttaaaacct acgattgtga
    34861 ttgttttcat aaacttttaa tctaaaaact ccggaatact aactatttat ttgtaatatt
    34921 tgtaataaaa ttaaaatcat aaaaaagttc taatttaaaa ttgatcattt ttatagtaat
    34981 tgctttttat caactactct aaccaatttt ccgtactaac tgttggtaat tcgttaaatg
    35041 tttctctttg ttcgtttgca agctttgccg caatagcttt ttcgacaaac caatttttta
    35101 cataaacgaa aattagattc tgcaaattgt ttactttttc agttttttgt ttttgataag
    35161 ctttcagtaa agtcatatat aatcctttgt cagcatcaat tccataagat tccagttctt
    35221 cataaacgac aatagtttgc tctaattctt cagctttttt ctttgcatta tgaatagttt
    35281 ggacggtttg ttgagcttca ctgtaacttg aagaaaatgt ttttatcaag tttaatacat
    35341 tttctggtac aaatgtattt atactgtcct cttgcatgat gtcgacgaag ttatctagca
    35401 aaacttgatt ttgtaactgg tcagtttcag tgtctattat gtgtctatta gtgtctatag
    35461 aattagaata gaatagattg tctgcgattt tcgcaattac ggttgagtcc ttgatatgac
    35521 tggattttgc cgtatttgcg attttcgcaa ttacggtaga atcattgatt tttcgcggtt
    35581 ttgccgtatt tgcgattttc gcaattacgg tggaatcagt attgtcttcc tcttccatga
    35641 ttttaaagac gtcttgattc gtaacttctg gcttgcctaa gtacaataaa ttaggcatag
    35701 attcaatctt accatcacgt ttatttactc ttcctctctt ctgtttcaga agacctgctt
    35761 tttctaattc ttttttgatc ttagatactt taccttcgcg gcattgtaac aatttcatta
    35821 attcttcaac tgtgaatatg aagaatatat tatcatcaga atctacccag ttatttttga
    35881 ctgaataatc aaatcgatct ttaagtagca tataagcaat ttttgcatca tttgaaagat
    35941 ccatgtattt ttcgttagta aaaaatacct taggcatttg ataatatttt tcacgataaa
    36001 tttcttttga tttgtaataa ttaaaatcat tcatagccaa tcctctttct gtaggatagc
    36061 gttttgttca aaactatgtt acaatgaatt aaacaaatat atctatccac tatatatttt
    36121 tctactgctg ataagtcgcc aaactttgtt aaagcagtag tcttttttta tcttttttta
    36181 gtttacctta aaaaataaat taaagcaaac cgctctattt cagggtttaa gctcatggta
    36241 actatatcac gatattacaa taacattata tcacgatatc gtaatattac aatatgaaga
    36301 taagcgactt atcagctttg atactactga tttttataaa aaaaatatca cgatattatg
    36361 atatcgtgat attgaatgta
//
LOCUS       DACDPS010000019        35605 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.019, whole genome shotgun sequence.
ACCESSION   DACDPS010000019 DACDPS010000000
VERSION     DACDPS010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.019"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(140..505)
                     /locus_tag="IWJ07_001027"
     CDS             complement(140..505)
                     /locus_tag="IWJ07_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332621.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            713..1195
                     /locus_tag="IWJ07_001028"
     CDS             713..1195
                     /locus_tag="IWJ07_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332622.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1392..2282
                     /gene="pstS"
                     /locus_tag="IWJ07_001029"
     CDS             1392..2282
                     /gene="pstS"
                     /locus_tag="IWJ07_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAP9332623.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            complement(2569..3843)
                     /locus_tag="IWJ07_001030"
     CDS             complement(2569..3843)
                     /locus_tag="IWJ07_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9332624.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTWEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHCYQSGISNAFKVSHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            4116..6587
                     /locus_tag="IWJ07_001031"
     CDS             4116..6587
                     /locus_tag="IWJ07_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9332625.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(6690..8426)
                     /locus_tag="IWJ07_001032"
     CDS             complement(6690..8426)
                     /locus_tag="IWJ07_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAP9332626.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(8423..9127)
                     /locus_tag="IWJ07_001033"
     CDS             complement(8423..9127)
                     /locus_tag="IWJ07_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9332627.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            9258..9761
                     /locus_tag="IWJ07_001034"
     CDS             9258..9761
                     /locus_tag="IWJ07_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAP9332628.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            9721..10059
                     /locus_tag="IWJ07_001035"
     CDS             9721..10059
                     /locus_tag="IWJ07_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAP9332629.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            10080..11498
                     /gene="ffh"
                     /locus_tag="IWJ07_001036"
     CDS             10080..11498
                     /gene="ffh"
                     /locus_tag="IWJ07_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAP9332630.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            11610..12188
                     /locus_tag="IWJ07_001037"
     CDS             11610..12188
                     /locus_tag="IWJ07_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAP9332631.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            12192..12713
                     /locus_tag="IWJ07_001038"
     CDS             12192..12713
                     /locus_tag="IWJ07_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9332632.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(12792..13346)
                     /locus_tag="IWJ07_001039"
     CDS             complement(12792..13346)
                     /locus_tag="IWJ07_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP9332633.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            13599..13874
                     /gene="rpsP"
                     /locus_tag="IWJ07_001040"
     CDS             13599..13874
                     /gene="rpsP"
                     /locus_tag="IWJ07_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAP9332634.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            13886..14137
                     /locus_tag="IWJ07_001041"
     CDS             13886..14137
                     /locus_tag="IWJ07_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP9332635.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            14262..15053
                     /locus_tag="IWJ07_001042"
     CDS             14262..15053
                     /locus_tag="IWJ07_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332636.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            15223..15744
                     /gene="rimM"
                     /locus_tag="IWJ07_001043"
     CDS             15223..15744
                     /gene="rimM"
                     /locus_tag="IWJ07_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAP9332637.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            15734..16495
                     /gene="trmD"
                     /locus_tag="IWJ07_001044"
     CDS             15734..16495
                     /gene="trmD"
                     /locus_tag="IWJ07_001044"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAP9332638.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            16631..16978
                     /gene="rplS"
                     /locus_tag="IWJ07_001045"
     CDS             16631..16978
                     /gene="rplS"
                     /locus_tag="IWJ07_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAP9332639.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            17236..17529
                     /locus_tag="IWJ07_001046"
     CDS             17236..17529
                     /locus_tag="IWJ07_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAP9332640.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(17617..17730)
                     /locus_tag="IWJ07_001047"
     CDS             complement(17617..17730)
                     /locus_tag="IWJ07_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAAX protease"
                     /protein_id="HAP9332641.1"
                     /translation="MYAPGTILLTIAYLTANRSLAFIIAIHILNNVLGFVL"
     gene            18372..20699
                     /locus_tag="IWJ07_001048"
     CDS             18372..20699
                     /locus_tag="IWJ07_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9332642.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            20825..21154
                     /locus_tag="IWJ07_001049"
     CDS             20825..21154
                     /locus_tag="IWJ07_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP9332643.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     AEIKTSD"
     gene            21194..22198
                     /locus_tag="IWJ07_001050"
     CDS             21194..22198
                     /locus_tag="IWJ07_001050"
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP9332644.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKTKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            22195..23624
                     /locus_tag="IWJ07_001051"
                     /pseudo
     CDS             22195..23624
                     /locus_tag="IWJ07_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
     gene            23830..25002
                     /locus_tag="IWJ07_001052"
     CDS             23830..25002
                     /locus_tag="IWJ07_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9332645.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(25164..25619)
                     /locus_tag="IWJ07_001053"
     CDS             complement(25164..25619)
                     /locus_tag="IWJ07_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332646.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQAAAEQASQQAQAAAP
                     EQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSKCYGN"
     gene            26209..26631
                     /locus_tag="IWJ07_001054"
     CDS             26209..26631
                     /locus_tag="IWJ07_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9332647.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            complement(26797..27990)
                     /locus_tag="IWJ07_001055"
     CDS             complement(26797..27990)
                     /locus_tag="IWJ07_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9332648.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            28214..28591
                     /locus_tag="IWJ07_001056"
     CDS             28214..28591
                     /locus_tag="IWJ07_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9332649.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            28579..28917
                     /locus_tag="IWJ07_001057"
     CDS             28579..28917
                     /locus_tag="IWJ07_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9332650.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            29051..29494
                     /locus_tag="IWJ07_001058"
     CDS             29051..29494
                     /locus_tag="IWJ07_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAP9332651.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            29756..30571
                     /locus_tag="IWJ07_001059"
     CDS             29756..30571
                     /locus_tag="IWJ07_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9332652.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            30581..31243
                     /locus_tag="IWJ07_001060"
     CDS             30581..31243
                     /locus_tag="IWJ07_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP9332653.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            31448..33112
                     /gene="alsS"
                     /locus_tag="IWJ07_001061"
     CDS             31448..33112
                     /gene="alsS"
                     /locus_tag="IWJ07_001061"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAP9332654.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            33125..33835
                     /gene="budA"
                     /locus_tag="IWJ07_001062"
     CDS             33125..33835
                     /gene="budA"
                     /locus_tag="IWJ07_001062"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAP9332655.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            33965..34462
                     /locus_tag="IWJ07_001063"
     CDS             33965..34462
                     /locus_tag="IWJ07_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAP9332656.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            34647..34907
                     /locus_tag="IWJ07_001064"
     CDS             34647..34907
                     /locus_tag="IWJ07_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332657.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(35269..35508)
                     /locus_tag="IWJ07_001065"
     CDS             complement(35269..35508)
                     /locus_tag="IWJ07_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332658.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT        11965 a         6278 c         7144 g        10218 t
ORIGIN      
        1 tacacaactg tttttagtgt ttggagagaa gaaaagaagt aaaaaataga aaaactagtg
       61 atagttttgg tgatataaga aaaacatata tgtataaaaa gaagaaagta gcttctcttt
      121 gtacatatat ggtttgtttt tatggatgcc gagctttttt ataagataag gctaccgccc
      181 ataacgaccc aaggatttgt ggaactaagt aaatacctgc aagaagcaag atcagctggg
      241 tcggttttaa aggatgatag aagtaacgcc attcagtaaa attggcgcaa aataaatagg
      301 aagtgacagt agatataata ttggcaatga ataaagatat aatacgcctg ttcctttgaa
      361 aataatatcc agcaaaaaga gataaaaata tcaatgtgat gaacccgctt acggaatgat
      421 aacgaaaatc aagcagtaag gcgaaatgca cataaggaac aaaagataaa agcaacggaa
      481 ggtttttcca gcaagttttt atcatctttc atcaccacct aacaagtgtt attattttgt
      541 tagttttatt gtaaattgaa tcctgcaata atcaattact ttttcattat ttctagatta
      601 aatgaataaa ctctcatgcc attatctcta aaaaaaaagt ttttccccct aattttttta
      661 gatagaaaat tgtatagtat gatgagagaa aaagtagttt gggggaacgt taatgagaag
      721 aaaagcagta atcgatcggc tttatttatt tttttggttt tttcctatcg ttttgctacc
      781 ttttacatgg gtaaatgctg ttgtacaagg tgcggagata ttaggcctga tccaaggaag
      841 cggatttgtg gtttctagga attttccagc tttgattgtt ttctatttag tgataagtgt
      901 aggtctctta ttaccaaaat ctctgatcaa tcactacaat tggcagctgc tttacttttc
      961 ttgcttatta gtatttacat ctatgttttc tttgctgcct cgcttgatta tcggccctaa
     1021 tggtttgagc gagagtggat tgaaggattt agcttattcc tccatgtatt tcttgacgat
     1081 caaaccatgg ttctatttag taattgcctc atttattctt gcaacatttt tttacttctg
     1141 gacagaaatc aggataaaaa gtgaacgatc tgtatctata aaaaataact attgatggtg
     1201 gatcatcagt aaaaaaacat atgagctgcg taaaatgcat gtttctcata tgcttttttt
     1261 attattatgt aaagaatgcg aaaaacgttt acatgatttt tacataactt aactatattt
     1321 ttacagcaac catacgaggg atttacagtc gactgataaa ctttagttgt aaataagaaa
     1381 ggagcaataa aatgaaaaaa gtattgttta ctctaggtat gatgggaatt cttttagccg
     1441 gatgcggctc aggatcagga aatacagatg gctctgccac acaaaataat tcaagtgact
     1501 caaatgaaca agtaaaaatc gttgcggtag gttcgacagc attacagccg ttagtcgatg
     1561 cagcacaaga aagctttgta caagaaaatc caaattatca aatctctgta caaggaggag
     1621 gaagcggaac tggtttgagc caagtcgaag ctggagcagt tactatcgga aactctgatg
     1681 tatttgcaga agaaagagac ggagttgatg cttcaaaatt agtggatcat aaagtagcag
     1741 tggtcgggat ggcaccaatc gtcaataaag atacagatgt caaagatatc accaaacagg
     1801 aattgattga tatctttaca ggaaaaatca ctaactggaa ggaagttggc gggaaagatc
     1861 aaaaaatcaa cgtcgtgaac cgtgcaaacg gcagcggtac ccgcgcgaca ttcgaaaaat
     1921 ggggactgga tggtgctacc cctgtgcagt cccaagaaca agattcatca ggaacagttc
     1981 ggcagctggt aagtcaaaca ccaggagcaa tcagctattt agctttctca tatctcgatg
     2041 attccacaca agcactaagt atcgacggtg tagaaccaaa agaagagaat gtagcagata
     2101 acagctgggg aatctggtca tatgaacata tgtacacgaa cggaaaacct tcccctgaag
     2161 tccaaaaatt cttagactac atgatgacag aagaaatcca agaaggacct gtcaaagaat
     2221 tgggctattt accaatcaca atgatggaag tagaacgtga tcacgaagga aatatcaaat
     2281 aactagcaga taaacatgcc acggtagtcg ttactattaa ggaaaaatca actatgtttc
     2341 cccacatata catttttcct ttaattgtga aaaatagttc ctcatatttt gccgtttttt
     2401 gagctaatac cgagggtcag ctgattgaac accgtttatt cgaaaaaaag agctacgaca
     2461 tagaactgtc gtaacttttt ttggctcttc gtcaaatcgt gttggtgaag acaaatttga
     2521 ataaaactta gtccgatatg cggcgcgttc tgtgcgccgc atattttttt agttttcaaa
     2581 gatcagacct tgttgtaaat agatccgtgc ccgaaagtta taaaagttgc gataaccaaa
     2641 cgcaattcgt ttaatcagct tgatcttgtt attcagcccc tcagtgacgc cgtttgagtg
     2701 ggacacctta aaggcatttg aaatacccga ttggtagcaa tggaagatcg tgaatttctt
     2761 tttaaacgac tgaggtaacg tagatgaaat tgttttaagg gtttcttgaa agccttccgt
     2821 acctcgttta acgaaataat agcgtaataa ttgacaaaaa tgatacgcag ttcgcaattc
     2881 ttcgttgtag cttaacagct catcaacgat ttctgtttga ctaattggtc gtttaaacag
     2941 tcgatgataa tgccgtttga gtggctccca tgtcgtggaa tctttgagta agagtttcca
     3001 atatcgcttg agccgacggt acttcttctg attttcagag tgtgaggttc gaaaacgatt
     3061 catgatctgg acacgtaatt gattgaagct gcgattgatg tgttggacaa tatgaaagcg
     3121 gtcagttacg atctccgcat ttggaaagac tgttttaagc aactgacaat agctggcatt
     3181 catgtccatc actaggtact tgacctttaa acgggccttt ctaggaaagc gcaggaagta
     3241 ttgagttagt ttgtaaagtc tgcgatcttc aaggatcgta aatagctgat tggttgtccc
     3301 atcaacacaa ataaaactca tggaaccaga aacagaattc attgatttga actcatccat
     3361 acacaacacc gtcggaagga agttcatacg tggctggtaa tgcttcgttg cttcacgcaa
     3421 gatccgtgtc accgtcccat ccgagacgaa gtacttctta gcgattagtt tacgtgattg
     3481 attttcgtac agttccatga gaatcgcctg cttcagttcc tttgttagtt gatgatgttc
     3541 atcaactaag tccgttttcg cagaaaaggt ccttccacaa tcatggcaga gaaaacgggt
     3601 acgttttaat tctaaaatag ttaacttttc tttgactttc aacatctgag tgcgtgtcgt
     3661 ataggaaccg ttgcggataa tacgaaccga tcgacagtgt tcacaatagg aagaagagtc
     3721 atccaatttt cctttaataa actgcgcttg aacgccttta tattcttttt caatcagcca
     3781 ttcttcatca aaaaataaat gtttatctgt taatccgagt aattttctag tatgattatc
     3841 cattgagaga ctcctttggt aggatgacgt tttggtcgac taaattttac caaaagtgag
     3901 tctctttttg agttgaaacg aagaaaaagt gttagtagaa aatcgccttc gattttccac
     3961 caacacaaaa tattatagaa cccttttttt ttgctatatg acaggaatga aaggtgtttg
     4021 caagcttaat gtgaggaaca ctttccatat ctgttctata ctggatagga gaagtttgga
     4081 aatatccaga aaacgaaatc aatcgaggta gccacatgaa gaataaatgg ataaatcgaa
     4141 gtaaaaaaaa gaaagtaaca caaagatcat cagataaagt catccgtctt tttccatcgt
     4201 ataaagaagg attaacggca gatcaagtca aggaacgaat agaaaaaggt gcagcaaata
     4261 attcagtcga tcccacattc aaaacaaatc agcaaatcgt attggaaaac atatttacgt
     4321 atttcaattt gatctttctt atcttagcca ttttgctttg tttagtagaa tcctataaga
     4381 acttgacttt tttaccagtt atcatagcca atacaggaat cgccattttt caggaaattc
     4441 gatccaaaaa gatcttggac gaactaaatg ttttaaatac agcaaaagtc aatgtcatac
     4501 gagacgggat agaacaaaca atagatatag aagaccttgt aatagatgac attgttattt
     4561 tagagactgg atatcaaatt ccagcagatg cagaggtcgt ggaagggaaa ctgcaagtta
     4621 atgaggcttt attgacaggc gaagcagatg aaataaccaa agaaataggt gatccattaa
     4681 tgtccggcag tttcattgtt tccggaaaag cttacgcccg attagacaaa gttggagcag
     4741 attcgtatat ttcccagctg acagtcaaag ccaagacaat gggagaagga gaacagtcgg
     4801 agatgattgc ttctcttaat caattgatca aatggattgg gatcatcatt attcctatcg
     4861 gtatcctgct tttttctcaa agttactttt ttaatggaaa cacgatcaaa gaaagtgtcg
     4921 ttgcaatgga agcagcattg atcggtatga tacctgaagg actgtacttg ctgacgacga
     4981 ttgcattagc attaagtgcg actaaactag caaagcaaag agttctttta cacaatatga
     5041 aaagcattga gacattggca agggtaaatg tcctttgtgt agataaaaca ggaacgatca
     5101 cagaaaataa aatgtctgtt caaaaaatga tcgtctctaa aaaacaagaa ataatcagtc
     5161 agtcagatca attggaagca tgcattgctg actatgcaaa agcgatggca ggagacaatg
     5221 caacaatgga agctgttaaa gcatatttta caaaaaccac tgatcaatcc tatcaccatg
     5281 ttattccttt ttcttctgtc caaaaattca gcagtgtagc gttggcagac aaggtgtatg
     5341 ttttaggagc gcccgaaatg gttttgcgcg atcaattgcc ggaatatgcg gaagagtttg
     5401 tttctttcgc tgaaaaaggc tatcgcgtgt tggtctttgg tgtgtacgat gggattcttg
     5461 aagaaccagt attgacagaa gcagtcacac cattaggcta tattctgatt gctaatccta
     5521 tcagaaaaga agcaagagcc acatttgatt acttcaaaaa acagcaagta gccatcaaag
     5581 tgatttctgg agataatccg ctcaccgtat ccaatgtagc tgtccaagca gggatttcac
     5641 atgctgatca gtacgttgat gcatctaaat tatcagaaga agaatatgaa gcagctgtcg
     5701 aaaaatatac agtttttgga cgcgtcaagc cagaacaaaa gaaaatcttc gttcaattga
     5761 taaagcgtaa caatacagta gctatgacag gtgatggcgt gaatgatatt ctagccatga
     5821 aagaagcaga ctgcagtata gcgatggctt ccggaaatga agccgccatg caggcctcac
     5881 aagtggtctt acttgattct gacttttcac gcatgccaga agttgttgca gaaggacggc
     5941 gcgttgtgaa caatattgag cgatcagcca gcttgttttt agtgaagaat attttctcct
     6001 ttttgctctc actgttttca gtgatctttg ctctgaccta tcctttagaa ccatcacaaa
     6061 tcacattgat tagtttgttt actatcggac ttccatcttt cttgcttgct ctagaagaaa
     6121 acaaaaagcg aatcagaggg aaattcatca tgaatgtgat ggaaaaagct gtgccaggtg
     6181 gactaacaga tatgatcgta gtcggtgcgt tggtgatctg cggggtgact ttgaatctaa
     6241 ataaaacaga tgtttctaca gcatctacca tgcttttgat tgcagtaggg ttcctcgttc
     6301 tctataaaat ctgttcaccg ctgaataaat tcagaagtca gatcattctt ttctgtgcta
     6361 gcggtaactt tttttcggtc atctttttgc ataagctgtt ttcaatcacc ggtatctcag
     6421 ctgtatcact tttgctgttg agcattctat ttttctcggc agattcgatt tttaggcatt
     6481 tgaccacatt ggtagaatat ttgtttgaac gtaaaaatga aaaagagcaa aagccgttta
     6541 gtcgcgtagc taaaaaattt ctatcgatat ttaaacgaga aaattgacga aaagtatctc
     6601 aaaaaagagt aaaaaagaac aaccactcgt gtatctttcg aagcaggatg agtcatccaa
     6661 ctttaaaacg cacgagcagt tgctcttttt tacattttcg gaatggtaac ggtgaaagtc
     6721 gaacctgttc caagatgact gtctacagta atcgtaccat taaggagttg cacgtagtct
     6781 tttacaatcg ctaaccctaa tccggtcccg cctgaatgtc ggcttcgcgc cttgtctacg
     6841 cggtaaaaac gctcaaagat ccgttcctga tcctcaatgt cgatacctat accaaagtct
     6901 tggacagaaa acgatagatc atctgttgcc tgatagcgga taataatctc gctgtcgctt
     6961 tttgaatatt ggacagcatt ttcaatcaga tttttgataa ttggataaag gatatctgtt
     7021 ttagtagtaa atggttcgtt ctttccaatc aatcgaatcg tcaaccgttt cttttcgatg
     7081 attggctgat aattttgaac gatttgctgg aaataaggtt ctgtataaat tgtctggatc
     7141 tcgtaagaat tctcgccatc ttttgacagc tggatgattt cccgaatcag tttatctaac
     7201 cggatcgcat ctttttgcat gatctcaaga aaagaagtca gagtttgcgg gtcatctttt
     7261 gcaccatcca gtaaagtctc ggtaaaacca atcagtgaag tcacaggagt ttttaattcg
     7321 tgagagacgt tgccaacgaa atccttttgt agcttttcta aacgtcgcac tttcgttaag
     7381 tcatacgcca cccctagaat ctgtccggta ccttctgaat tagtaaagta gcgcaaactc
     7441 aagtctaatg tctgccgacc ttctgtaagg gtgatctcct gatggatcag cggatgctca
     7501 ggagtcactt gatgaatcag ctgaatgagt tttggctctt gaatgacttc tgtgtaccgc
     7561 tgttcaggtt gatagcttac cacgtttaaa tgtagctgca tctttggatt aagcagtagt
     7621 aaacgagaat cagcatcatc aatcaggaat acgccaatca tcagttcgtt cagtaacgta
     7681 tataattgct cttctgtggt agtacacgcc cgatacatct tactcatttg ttcgctcaac
     7741 gcattaatcg tctgatacaa ttcttcccat tgatcgggtg tttgcatgat tacttccgcc
     7801 tgattaggat tttgcaccat tttttttagt acgggcagca cggttttcaa tggctggttt
     7861 ttttgataga tcagataata gatcattcct gcaagaacaa ggaagaaaga cagaaagaag
     7921 aaaaagaccc aacggcgaaa actctcagtt ctcggtaaaa aaacgcttgt cggttctgcg
     7981 atacgtaaaa tggcttctaa ctggccactt tttttgactg gtaatgcgac atataaaaga
     8041 tcctcattca aagtagtact tttgcgcaaa gcgctaccta ggttcccacc attcaatacg
     8101 gctttgattt cgggacggga gtttcgctgt tcattcaatg tttcattgtt tgtgtcaaat
     8161 aagatctttc cagtagcatc cattaaagta atccgttcgt tcgactgatg gacgaattct
     8221 tccaaagcag ttttgttttg cgctgcttgg aggttttcga catctaattg ccggatcagc
     8281 agttgtgctt tttttgtcag gtagctttct tgctgttcca cgatctgctg ttgaaaataa
     8341 tgggaaatca attgccagcc accaaaaaac aaactaacta aaagaataag aaagaagaga
     8401 cgttcataaa tttttttcca tttcatcgtt tgggttcctg aaacttatag ccaaatcctc
     8461 taatagtcac caagtacttt gggtgttttg gatctcgctc gattttttct cgcaaatgac
     8521 taacgtgtac atctacgatt cggctttgtc ctgcgaagtc aaaattccaa atgcggtcta
     8581 gcaatgtgtc tcggtcgatc acacggtctt ttcgtttcat gaaataaaca agtaattcaa
     8641 attctttcgg tgttagttca atcagatggt cgtctacggt cacttgataa ttggtcaaat
     8701 cagcagtgat ttgtccaatc gatagatatt caggctcagc ttcatctttt gattgcttac
     8761 gaggttcaat tcgtctgaat attgctttca tacgggctaa tacttcgcga ggactaaatg
     8821 gtttggtcaa atagtcgtca gcacctattt ctaggccaat gatccgatcc acttggtcat
     8881 ctttagccgt cagcatcaaa atcggtgttt catttttttc ttgacgcagc cgctgagtga
     8941 tctccatgcc atccatcgtt ggaagcatca catctaaaat gatgaaatca aatgattgtt
     9001 ctaatgctaa ttctaatcct tcgcctccat ctgtggcact ggttaccttg tagccttctt
     9061 tttccaaatt gaacgtcaag agcgttacaa tagaaggttc atcatcaaca actagtattt
     9121 ttttcattca aatctccttt aaataggttt cattctgttt atattttagc atagaatcag
     9181 tacaaagggc tattttacaa aagaaataaa tggtataata ggaaggcact ttgctgaata
     9241 gaaaggaaga gcagtcaatg attggaatca gtgcatgttt aggcggtctg gcctgtcgct
     9301 atgatggaca gaaaaaagaa gtaccagaat tgatggaact agttgaaact ggagaagctg
     9361 tcatggtctg tccagaagtg atcggcggat taccaattcc tagagatccc gctgaaatca
     9421 ttggtggaaa tggattcgat gtgtggaaag atcaagcaaa agtatggacg acaagcggcg
     9481 aagatgtaac ggaagcgtac aaaaatggcg cgatcaaagc ctatcaaaaa ttgcaggaaa
     9541 aacacattga tctagtcatc atgaaggaaa aaagcccttc atgcggaacc cattcgattt
     9601 atgacggaag tttttccgga ataaaaaaaa atggtgccgg agtggctact gcttatttta
     9661 ttcagcaaaa aatgactgtg ctctctgatg aagaatggct gaaaactaga ggtgagcaga
     9721 atggaaatcg aaaagaccaa tcgaatgaac gcgctctttg aattttattc gaccttattg
     9781 acagaaaaac aaatgaacta catggaatta tattatgcag atgatttttc attaggtgag
     9841 atcgcggaag aatacgaagt aagccgacaa gcagtatatg acaatatcaa gcggacaagc
     9901 aagatcttgg aggattatga aaaaaaactc catctttttt ctaattatat tgttcgagaa
     9961 caggtattgg aaaatatgac tgcatatatt acagataagt acccggaaga taaaaaacta
    10021 cttgaatatg tacagcaaat tcaagcaatc gaagaataaa taaaaggaat gaagaaaata
    10081 tggcatttga aagcttaact gaacgtctcc agcaggcgat gggaaaaata agaaaaaaag
    10141 gaaaagtatc agaagctgac gtaaaagaaa tgatgcggga aatccgcctt gccttactag
    10201 aagccgatgt taacttgcaa gtagtcaaag attttactaa gcgcgtacgc gaacgtgcgg
    10261 taggggcaga agtgctggaa agtttatcac ctgcacaaca aatcgtaaaa atcgttgatg
    10321 aagaattgac gatcacactt ggttctgaaa cagcagagtt aaataaatca ccaaaaatac
    10381 caactgttat catgatggct ggtcttcaag gggcaggtaa aacgaccttt actggtaaat
    10441 tagccaatta tttaaagaaa aatgaaaatg ctcgtccgct cctaatcgca ggggacgtat
    10501 atcgaccagc tgcgatcgac caattgaaag tattaggtca acagctagac gttcctgtat
    10561 ttgatatggg aactgaagtc agcccggtag agatcgtccg tcaagggatg gagttagcaa
    10621 aagaaaagaa aaacgactat gtattgatcg atacggcagg acgtctgcat atcgatgaaa
    10681 cattgatgga tgagttgaaa caaatcaaag aattgacgca accaaatgag attttgcttg
    10741 ttgtcgatgc catgactggt caagatgccg tcaacgtagc tgatagcttc aaccagcaat
    10801 taggtattac aggggtcgtc atcactaaac ttgatgggga tactcgaggc ggggcagcac
    10861 tatcgatccg ctcagtgaca ggcgcaccaa tcaaattcat cggttctggt gaaaaattga
    10921 ctgacttaga agtattccac ccagatcgta tggctagccg tatccttggt atgggggata
    10981 tgctgacatt gattgaaaaa gcgcagcaag actacgacga gaaaaaagcg gaagaacttg
    11041 cacagaaaat gcgggaaaat tcattcgatt tcaatgactt tattgaacag cttgatcaag
    11101 tgatgggtat gggcccactg gaagatctaa tcaaaatgat cccaggaatg aatcaagtcc
    11161 ctggaatcga aaacgtcaag gtagatccaa aagatgttga acgcaaaaaa gcgatggttt
    11221 attctatgac gccagcagaa agagcaaatc ctgatctgtt gaacccaagc cgtcgtcgcc
    11281 gaattgctgc cggttctgga aacagtgttg ttgaagtgaa tcgaatgatc aaacaattca
    11341 aggaatcaag aaaaatgatg cagcaaatgt ccaaagggga tatgaacatc ccaggtatgg
    11401 accaaatgtt tggttcaggc gtcaaaggaa aactaggcaa aatggcgatg aatcgaatga
    11461 tcaagaaaaa taagaagaaa aagaaaaaac ggaaataaga tgaaaaaaac agcttcggta
    11521 cagactgaaa atgtctactg ccggagcttt tctatcagca atttttattc tttactaata
    11581 caattactct aaacgaagag gtggcacaga tgagatgtcc gtggagtgaa caaacagaaa
    11641 gtatgcggca atatcatgat actgtctggg gtgttcctga atatgatgac caaaagctgt
    11701 tccgaaaatt gatgcttgat attaatcaag ccggattgag ctggcagacg attttaaata
    11761 aaagtgaagc attcgatgaa gcatatgatc gattcgatat caaaaaagtc gcaaattatg
    11821 gagaaacaaa aatcagtgaa ctaatggaaa acaaaggaat tattcgtaac cgaagaaaaa
    11881 tcgaagcagc cgtccaaaac gccaaagcag tcttgaaaat tcaagaagaa atcggcagtt
    11941 tttcagagta tctttggtcg ttttctaatg gaaaaatcgt tgattcttct tatcattcac
    12001 aagaagaagt cccaacaaca agtcctcttt cagatgaatt agcaaaagat ctaaagaaaa
    12061 gaggatttaa atttatcgga agcacaacaa tctatgcttt tttagaagct gtcgggatca
    12121 tcaatgatca ccttgacagt tgtttccgca aagaagaggt cgtacgtatc ggcagaaagg
    12181 gcgaataaag catgcgaaaa atcatatttt atggtgctat aagtttagat ggctatttgg
    12241 caacgaaaac ggatgatctt caatggcttt ttgacactcc aacaggtaag caaactactt
    12301 atgaagcttt ttatgaaaca atcgatacga ccatcatggg cagaaaaact tatcaggaag
    12361 caaaaaaata tttggagacc gaagcaattt atccagagaa aaagaatttc gttttttcca
    12421 cccacgcaca attacaattg ccagatgctg aggtcgtaaa cgaagatcct gttgcttttc
    12481 taaaaaatct acaaatgaca gaaggaaaag atatctgggt tgtgggtggc ggtcgattga
    12541 tcaaaccgct attggaacat caaatgattg atgaatggta tatccagatt acccctgtat
    12601 tattagggga cgggattcct ttattccaaa aaggaacata tgaagcgcgt tttgaaatgc
    12661 tagacacgac acgattcggt gaatttatcg aattgcatta tgtacgaaag tagaagtaaa
    12721 taaaataaaa aaggcaacta tatgcagaaa tgtcctgtac atagttgcct tttttattac
    12781 cgttatgctt tttactctgt taatttgtta gaaaaatcta gatacgcatc taaaatcgtt
    12841 tggaagaaac ttaatgttcc ttcaacgata ttaccatttt catcaattag attcgcaacg
    12901 tttccgatat atgcttccgg ttgctgcaag gttggcacat tcaaaaagac aagtgattgg
    12961 cgtagatggt ggtttgcgcc aaagccgccg atcccacctg gtgatgcagt aacgactaaa
    13021 cctggtttat gatcccaaac actgtgtcca taaggtcttg agcctacatc taaagcattt
    13081 ttcaatactg ctggcacaga acggttgtat tcaggagtaa cgaaaacgac accgtctagt
    13141 cccctcactt cctcacgaaa acgagtccat tcagcaggag cctgttctgg agtttcgaga
    13201 tcctcattgt aaaatggcag atcatcgatt tttacaaata cactttcgta cccttcagga
    13261 agcaatttcg caaaagcttc tgctactttt ttgttgtaag aatcttttct taaactacca
    13321 ataaaaaatc caattttttt tgtcatacga tttttcctcc ttcaaactat ctataataat
    13381 cttacaaaag ataagtaact ggagcaaata aaaacactta ctgacaaaag ctacatagaa
    13441 gatagattac ttatttttaa caaaaaagaa gtctaaaatc ttctattttc taaaaaaaat
    13501 cgaatttaat caagctgtaa agaaaaaaag ctttacaggg taaaaaaaaa ctgttacact
    13561 acatcttgtg aataaaacaa tggaggtgga aataaataat ggcagttaaa atccgtttaa
    13621 aacgtatggg ttctaaaaag agtccgtttt accgtatcgt cgtagctgat tcacgttctc
    13681 ctcgtgatgg acgtttcatc gaaactgtag gtacttacaa tcctttgaaa gaccctgcag
    13741 aagtagtttt aaaagaagac ttagttcttg actggttatc aaaaggtgca caaccttctg
    13801 acactgtacg taacatcctt tcaaaagaag gcgttatgca aaaacaccac gaagctaaat
    13861 tctcaaagaa ataaggtgac cggatatgaa agatttaagc gacttagtct taacaatcgt
    13921 tcgtccgcta gtcacttatc ctgatcaagt caagttagac gtcgtagagt cgaaagactt
    13981 ttacgagtac aatctaacag tggctcctga agatattggc cgtatcattg gaaaacaagg
    14041 gcgtgttgct aaagcaatcc gcacaatcgt ttacggtgtt cggatcaatg caccgaaaaa
    14101 agtgcgtttg aatatcatcg atgataagga aaagtaaata aacgtgctgt actcgaattt
    14161 tcgagtgcag cttttttgtt ttttttatta attgaagaaa tgattatttg gcaaaacaag
    14221 caagaaagcg tatccttata ggaggaggga gtgaacagcc aatgagaaag aaaatgttat
    14281 ttattccatt tgctctattg ttaagcgctt gtagttcggt tactcaagaa tcagacaata
    14341 cacatctatc aagcagtata tctgaaactg ttaccagtcc agaaaaaagt agtaccaaaa
    14401 cggcttccac cactgaacaa acaatggcta cctcaaataa tgagaaaaag actgctttag
    14461 accagttgaa agaacaacag ccaaatgttc ccatgcctct agatgtacca gtctcttcag
    14521 gctatctcaa tatcgcagcg acacatacaa agcaaggtta ttctatttta tattacagaa
    14581 cagatcgtcc cctcgggttg aatgctgacg aattgaatca agaaacgcct attgcaacct
    14641 atctatatca atatgggttt gcttctagtc aagaaacaat ccaagttttg cagccatttg
    14701 agatcgacac aaatggtcag caagtggact taggttctcg aatcacaggg tatcagcaag
    14761 gtgcagcagg ttcaagtttt cttgaatggc aagaaggaaa ctggcgtatt cgaatccgtg
    14821 ggaataatat agaaggccaa gatcccttac tattagctaa agaaatcgta gcttatttgg
    14881 aagaaaacag tctaccagca cctgaacaat ttggtaaaat tactgttgat atgggtgata
    14941 cgacgaatcg agccgtagaa gtaagctggc aagaaccgaa aaatgcctat acgatcactc
    15001 atcaagatcc tatgtctgcg ttgaagatgg cagtttctat gaagaggttg tgaaatcagc
    15061 gcgctgatct gagcccatca tttaactcca agtcacagga agtaatcaaa aatagcacgc
    15121 cgcatttttg cataattttg gcttaatacc ttaaaagaaa actagaattg cctcttcaca
    15181 catgctaaaa taaaacagac aaaaaagaaa tggggatata agttgactga atacttgaat
    15241 gtaggaaaaa tcgtaaacac acaaggtatc aaaggcgaag tccgtgttat ctcaacaacg
    15301 gatttcccag aagaacgtta caaaaaagga acggtgctaa cactattcca agacggaaaa
    15361 gcacctgtgg aattaacagt caaaagtcat cgcaaacata aaaattttga cttattgagt
    15421 tttgaaggac atccttctat caatgatgtg gaaaaatatc gggatggcat attaaaagtg
    15481 tcaaaagaca actctgtaga tctagctgaa aatgaattct attaccatga gatcatcggt
    15541 ctgcatgtat tagaagatgg aactgagtta ggtaaagtaa aagaaatctt gtcaccaggt
    15601 gccaatgatg tttgggtcgt tcaacgtccg aaaaaagctg atatattgct tccatatatc
    15661 gcttctgtcg tcaaagaagt cgatttagaa gctggagtgg tccgtgtaga aattccagaa
    15721 ggactgattg acgatgaaga ttgatgtatt gactttattt ccaagaatgt ttgaaggtcc
    15781 tatgggggaa tccatcatcg gtaaggctgt agataaagga ttattagata tcaatatctc
    15841 taacttccgc gattattcag ataacaaaca tcagacagta gatgattatc catacggcgg
    15901 tggagcaggt atgctgctca aagtccagcc catctatgat aacatccaag ccattgaaaa
    15961 agctgcaccg gatgtcaaaa aaagagtaat tcttcttgac ccagctggta aacgattcga
    16021 tcagaaaatg gcagaagaat tttcacaaga agaacactta gtattcattt gtggtcatta
    16081 tgagggatat gatgaacgta tccgcacatt agtgacagat gaagtttcct taggagatta
    16141 tgtccttact gggggagaat taggtgcgat ggtcatgatc gatgcaacag ttcggctttt
    16201 gccagatgtg ttagggaatc agacttcagc tcaaacggat tcttattcaa ccggtttatt
    16261 ggaacatccc caatatactc gcccggcaga atataaaggg atgaaagtac cagaagtatt
    16321 gacaaatgga aatcataaat tgattgaaga atggcaatta aaagaatcac taagaaggac
    16381 ctatcaacgt cggcctgatc ttttggaaac acttgaatgg acacctcaaa tgacgaaatt
    16441 tttagaagaa atcaagaaag aagaacaaga aaaaacagca gatgaagcca agtgactttt
    16501 tctaaaatct tctttacagt aggttgataa tatgttagta ttttatggtg agtgcaaaag
    16561 cacttaacta ttacgatatt ccgctgtgag aaaagctcat aagaatattt ggaataagga
    16621 gaagaaaacc atgaatccat taatcgaaga attaacaaaa gaacaattac gttctgacat
    16681 cccagcgttc cgccctggtg acactgtgcg tgttcacgca aaagttgttg aaggtactcg
    16741 tgaacgtatc cagttatttg aaggtgttgt tatcaaacgc cgcggtgctg gaatcagcga
    16801 aacttataca gtacgtaaag tttctaacgg agtgggcgtt gaacgtacat tcccattgca
    16861 tacaccacgt gttgcaaaaa tcgaagtagt tcgctacggt aaagtacgtc gtgcaaaatt
    16921 gtactaccta cgtgcattac acggaaaagc tgctcgtatc aaagaaatcc gtcgttaatc
    16981 attgttatca gactgagaaa gaacccttgg tattcaaggg ttcttttttc ttagcatgta
    17041 atttgaacac cctgatccag ttttttcttt aattattggt aggggagaat aatggattag
    17101 aataaatcta cctataagta gtagtaaaaa aaagagcact atgataatat ctttcttaag
    17161 agtaaccgct ttaataaaaa atccagaata ttctaacata aatggagtat tcttagaatc
    17221 aaaaggagaa agaagatggt ttatacttat acggagcaag aaaagatgaa agttttagac
    17281 acattcatga atgatggaaa agtaaaacaa gtaccaagtt caacaaaaaa gaaatacatc
    17341 ctattgaaag aagtcttgaa acgctttgac catggagtga cttatacaga aacagaggtt
    17401 aattcgatac tgctgaacgt attttctagt ggcgattatg tcgaacaaag acgttatcta
    17461 atcacatttg gctttttcaa aagaagttca gacggtcggg catatcagat gatgggaatt
    17521 gaaaattaga agttttaatt tatactcatt gaaaaaagtt caagtatgcg atattggttc
    17581 tacaccgcat acttgaactt tttttgtcat ctgtatctat aatacaaagc caagaacgtt
    17641 attcaaaata tggatagcta tgataaaagc taatgagcga ttcgcagtta aataagcaat
    17701 cgtcagtaaa atcgttccag gagcgtacat gaaaaaatca attggatgcg ttacatgaat
    17761 aaagataatt ctcttttatt tgtacgaata aataccaaaa cgtacctcct aaaattagat
    17821 tttggacaga tgccctttaa aaagataaca agatttcgtt cttgaaacaa tatatatgct
    17881 tttattccaa tgaggttgtg aaaacagtat tttgtcctga gcgttagaaa attgaaaatg
    17941 gggaatagca actcgtactt taccattttt taagtgaata caagatcagc taattgaaca
    18001 ccgtttattc gtgatgaggg acacaacact gcagtcgaca catttctctt ttatgttcaa
    18061 agataacaaa tatttaaaac aaagaaaaat agtagataat cccgtcctaa ctcttttatt
    18121 cttccctcat agcttccaaa tttaagcaat acttcaatta atcaagtttt atcttttttt
    18181 ttcaataatc gtaaaaaaag agtatttttt atttaaaaat aaaatctttg tattaaagga
    18241 caagactaaa ataaccttag aataaaaaga gaaaaaaagc cccaaacttt ctatacatca
    18301 ataaaataat aaaaattaaa tttagaataa cgtgtaaagg attatttgat gattttttat
    18361 tgagaggagg gatgaaaatg attaagaaaa ctcaaaataa aatccaacgt atatgtcaaa
    18421 aacattttgt ttctagactc aaagctgtat ctaaaagaaa atacttacat cccattatga
    18481 tttcgatcat cttgattttt actttcttag ggagcttatc aagtatttat gtaaacgttt
    18541 atacagaaac gtcaaatttt gctaaccaaa cgacagagga gacaaatagt ccaccctatt
    18601 ctgaaataca gtccatagaa agcaccaatc agtctagcaa caacgagaca acctcaacca
    18661 catcctcgac tcaactgatt gatccgacag aaaaagaaat cacagcagca aaagcagcag
    18721 gggagaaagc ttattcagaa acaggtagaa gccagacatt tagtactgtt gcggccactg
    18781 cgaatttcaa cggacatacc ataccagatg gatatacagt gatatatgcc aaagatggag
    18841 tcattgttgc ggttaacgga tcgatgagtg cggataaaac tgcgttacaa ggcgtaacca
    18901 aagcaatcgc ttttattgat aaccgtccag atatccataa tctgtttatt ggctccacag
    18961 taaatggtac tactatcaca ttcccaaaaa gtatctatat gggggtggct accgctggaa
    19021 atacgatcga ttcagttaca cccaatgcgg ataaaagtta ttcttttaca ccctcaacat
    19081 ggggagggct tcctgccgga ggaggtatga gtactttttc aatcggtata cagttagcaa
    19141 atgatattcc attcacgggg tactcgatca aaaatgggca gatcgttgat ggtaagggaa
    19201 atgtttcaaa ttttccaacc tatcaagcag caacggttca ggtcaatgcg aatcggacag
    19261 gaactttttc ttatacagtc aatggtggag cagcaaaaat cggaacgtac gggactacat
    19321 tatctggaat ccatgtgggt gacaaagtgg ttatcactcc aacagcagta acgggctatt
    19381 cttttgcaca aacaaacggt tctttcacag ccctagaaag taacgtcgat acggtgacct
    19441 atacaggcaa acaaacacca atcactatta agtatcaaga ctattttgga cagatgattg
    19501 ctccttctaa aataatgaat cttacttacg gaagtgctgc acaagacctg acgactaata
    19561 cgcctactat caacggatat acctttacag ctgtttcagc gacagaaaca aagaaccaat
    19621 cagcaatgag tgttttagct agtttagata caaatggaaa tgtaatcgta aaggacgcaa
    19681 atggaaaaca gatttctgaa gtcatcctat attataagac aaatgcaaca gtatcaatca
    19741 atgcaaatgg cagtaagtac tatgatggtc tttctgttgt accgatcgtt aaatatgcct
    19801 ttaatgataa aacagaatca acatcccttt taaacaatga tctcagtatt gatcaaatca
    19861 catggaatcc aactgatttc aaagcgatga atgaaaaggg agttgaagta actgcgccta
    19921 ctgaaattgg aacctatacg tcttggcaat tgacaaaaag cggactagct aaactagctg
    19981 ccagaacaaa ctatttattt actatcgtgc agacatctga tgtctatacg attaagcaaa
    20041 tcagcggtgc ggtagagctc agtggcagta agacatatga tggaaaagca ggtgtcccgt
    20101 caatccatgt caagctagca gagggtgtca ctagttcgct tactccagta caagtggcac
    20161 ttagcagtat ggattacaca gcagatgcac aatcagcaaa aaatatggtt aatgctggca
    20221 gctatacaat taaactaacg aatagcggaa tagacaaagt caaagcagca aatcccaagt
    20281 tgtcatttac tgatatagcc aacacatcag gaacttatac catctataaa gctaacgcag
    20341 tgattactgt cgatgacgct gcctttaatt atgatgccca aagtcactcg atttcggttg
    20401 gaaatctcca cgttaccggt gttgtcttag gcgaatcatt ggattatacc ctaacagtta
    20461 acagccggac agatattgga tcacaaatag taacgattag tttaggcgaa ggaagtgtaa
    20521 acaataatta tacgattatc gcaacagata ccgctaagtt gacgatcaat ccgactctct
    20581 tgcttccttc gacaggtggg ataggtctac tgccctttgt ctttttagga ctgatcttta
    20641 ttttttctgg cttcttttat tttatccatc gtaagaaaaa agcaggtgaa cagcgatgaa
    20701 aaaaacaaaa aatgtacgtg tggatatatt tgctgttctc ttttttatcc tactggtctt
    20761 ttttataggt tctccgactg tgtcagctag agaatcacct attacattta ctttgcataa
    20821 tcgaatgttc gaaagcgata agatgccaag tatcagtcaa aacgacggaa taaatatccc
    20881 ggataacttt caaggagtaa aagggttgaa cggcgttact ttcgctatat atgatgtctc
    20941 ggatgaattt tacaagctgc ggtcagaagg aagtagtgtg gaagacgcac aaagaaaact
    21001 agcacaaaaa agtgatagtg aaaaaatact ggcagaaaat gtgacaaaaa cagtagacga
    21061 ggaagagggc attgcttctt ttagtgcatc ggacaaagat gagcaaggtc gtgacgctgt
    21121 ttaccgattt gcggaaataa agacttcaga ttaagtaaaa gaaaaatcag caccatttgt
    21181 cgttgtgctt cctgtgacag attcatctaa tcataaattg acgaccatcc acttgtatcc
    21241 aaagagcgaa caaaaaatac ctgctgcact cacattaaca aaaacagtag agaataagca
    21301 gacagatttt gcagatgggg ataaagttcc ttatctcatc accgtaacaa ttcctgagaa
    21361 catcaacgag ataggaacct atacgatcaa agatacagct gatcctcagt tatggctaga
    21421 accagaaacg atcgattttt tgattggtgg agacaagatt catacatttc acacacaaaa
    21481 aacaaaacat ggttttgtct tatcagattc tggaaaagat cttagttcgt ttgctgggaa
    21541 aaaattaacg atccgttacc aaatgacatt gaaagaaaac agtgaaaaaa cgacatttga
    21601 taacgatgta cgtttgacga ctgacaatca aacagtagaa acacatcttg ccatccaaac
    21661 aggaggcaaa caatttgtca aagtggatcg gcaaacgaaa caaaaactag cagatgctca
    21721 atttctagtc aaaaaaaatg accaatactt agcgcaagaa aacgggataa atcattgggt
    21781 agccaaagat gattcaagag caacgatttt tacttccagt aaagatggaa cattttctgt
    21841 ccatggattg tcttttggaa gttacgaact agtggagatt aaaccgccta aaggatatat
    21901 attgagtcaa tcaaccattc tctttgaagt tgaaaaaggc agttatgatc cgcttcatcc
    21961 tataccattg gaaatcatca acgaaccaaa agcaaatccg acgaataatg gaaaaacgcc
    22021 agttcagatg cagacaaatg gtccatcaaa tgaaaaaaat ggaggaagct ttccaaaaac
    22081 aagcgaagag atgcttggag gtttctcaat tttagggttg ttgctagtcc tttcaacagg
    22141 gacagcttgg ttttataaaa aacaacaaaa aggaaacaat aggagggaaa taaaatgaaa
    22201 aacatcaaaa caaaaatatg gacgttttta ggaactgcta tcatgctgct tcctttcgtt
    22261 ctcggcctag gaactgcaga ggtttctagg ccgtatcacc tacaccagaa aatgttacgg
    22321 ttaatttgca taaattgaaa ttcacttcag caccagaaaa tcaaataaac aacggaacgg
    22381 aattgacatt tccgaatagc gaaccgttga acggcgttga attcaacgta tatgacatca
    22441 ctgctacgta ctatccaagt aaagacacag cagttccagc agacgctaca cccttcgcaa
    22501 gtgtaacgac ttctggtgaa ggattagcta acctgactct tccaggtaaa tctgatggga
    22561 aagatgctgt atacgtcttt gtagaaacac caaagcccgg tgtcgaaacg tcgccaaata
    22621 tcgtgttaag cttacccttt tataacccga attcatcaga ccctgacaaa cctttagaca
    22681 cgattcacct atatgctaaa aatgtcatca aagaaagcaa tatcaaaatc cagaaagctg
    22741 ggaacttttc aggggtagat aagttagcag gggcgcaatt ccagatcaaa aattcagaga
    22801 atcaattcgt gaccggtttt gatgaaaaca ctggtgtcgc tctctatgat cctgctgagt
    22861 cagcgttgac attcacaact gatactgaag gatccatcag tgtcaatgga ttaatagacg
    22921 gcaattatac tttagttgaa acaaaagcac cagatggtta tcaattaacc aatccacaaa
    22981 aagaaactac ttttatagta aaaaacggaa aattgcaaca aacaaccggt atcatccatg
    23041 gcgcagatga agccaatctt gggaatccaa acggtgtagt caatcgggaa aatacaacta
    23101 cggataacac gattactgta gaaaatgtcc aaaactacgg tgatttccac ttcatcaaac
    23161 aagatgtgaa cacgcaagaa aaattagccg atgcagaatt ttctattcta agagcaaaag
    23221 atccaactgt taaattaatg aaaaaaatag caaatcttgc ctcaggagaa tacccatatg
    23281 cttggtcaga tgaagtcaca ggttctgact gggaagaagt gaaattgatt tctgattcag
    23341 aagggaaatt ttcaatcgaa gggctgaaac aaggggcata ctgccttcaa gaaacaaaag
    23401 caccagaagg ctacgcatta cctaagaatg aagctgcgta cactgaattt acagtagatg
    23461 atgatccaac aacttctgat gaaacaacga tcgataacca acctaaagga atcttgcctt
    23521 caactggtgg tatggggatc atcgtattca tcttggtagg tactgcttta gttggaggag
    23581 cagtgatcta cttcaaaaaa cgccatcaag aaacagaagc ttaaactatt ggaaacaaaa
    23641 atcatacgat aaataaacga tagtgattca aatggggaga aaggttgtga caagtggtcg
    23701 gtctcttggt attcgctcaa caaacggcaa aataggagga actattttgc cgtttgttga
    23761 gcgaatactc aggtcaaggc gctgatttca caacctcttc atatacatat tagaaaatag
    23821 gtggacatga tgaataaaag cagaataaga atactggaca tcttcatggc aatcatttta
    23881 gtcgttggga tcgggatctt ttcttatcct tttgtagaag attcactgaa tgattttctt
    23941 gcccagcaaa tgatcatcca ttatcaaaag caagcatcga ataagaattc agaggaaata
    24001 aaaaaacagc aggaaaaaat gacaaagaaa aaccaacagt tggcagagca aaacgtatca
    24061 ccagggatag gcagtttcaa ccaagcagtt gatgcaaaag cattaaaaga tttacctagt
    24121 aatgcctttt ttatggaaca tatgcttgga gtcatcgaaa tcccaaaaat caatgtcagc
    24181 ttacctatat tcgatcagac aacggaaata tttttgcaaa aaggaacgtc attattggaa
    24241 gaaagttctt atccgactgg cgggaaaaac acccatgccg ttctttctgg gcatcgtgga
    24301 ctcccagaag caaaattatt tacagatttg cctaagttga aaaaaggaga ccagtttttc
    24361 atccagatca atggaaagac attggcgtat caagtagaaa aaatccaagt ggtcttacct
    24421 gatgaagtag attctttggg tatccaaaaa ggcagagatc tcgtgacttt gcttacctgt
    24481 actccttata tggtcaatac acatcgactg cttgtgacgg ggcatcgtat tccttatcaa
    24541 gcaaaagagg caaaaaaagc gatccaagga attgatcaat ggaaaaaatg gaaatttttt
    24601 atttggttta tcggtatact acttggatca atcggcttag tttggctact catcgcttat
    24661 ttagattctt tggccatcgc taagcgaaac tatcctttat ctttttatgt aaagaacaca
    24721 aacggacgac caatcgaagg aatggtcttt tctgtaaaaa cgctcaacgg caaacactat
    24781 attactagag aaaaagttcc atttgtgaaa gcttctgatg aatacggact agtgagattt
    24841 tctgatttaa aaggcaggaa ctatcgcttg caacatgagg agctcctgct taaaatccat
    24901 gtaaaacata aacattcgaa acagttttca atgaaattga aaaaaggaag atataaactg
    24961 cgaaaagaaa aagaggtgta ctatttgatt gagaaagaat gaatgaagat gtccaaaaaa
    25021 gcagagggaa cagactaaat gaaaaaagag agaagctgac gaaatggacg tgtaaactgc
    25081 tatttttggt ttactatctg gaacgccagc ttctattttt ttatgtttca gaagaatttt
    25141 attattatag tataactttt gaattagttt ccataacatt tggagcaagg ggttaaacca
    25201 cgagcttgag cttcgcttaa agtggcaggg gtatacgtac catttccaca tttatgtgta
    25261 tggtattttg aaccggtcgg tgtgacatag acagtttgtt cattattttg ctcaggagct
    25321 gcggcttgtg cctgctgaga ggcctgttca gctgctgctt gctgtcgcgc ctgttctgct
    25381 tgttgctgtg cgattgcttc ttgttgcgct tgttctgctt gttgtcgtgc aatttcttct
    25441 tgctttttcc gttcttcttc tgcttctttt gctttgattg tggaatcaat tgcagcaaga
    25501 cggcttgtca actcttgttt gttttcaggt agcgcttgga tagctgctga agcagttgca
    25561 tagttttctt ttgtaggatt tgcttcagct tgtgacaaag cagattccgc ttctgtcact
    25621 tttttttcta gtgcagcttt ttccgctttt tgtttctctt cttctttttt cttttcaatt
    25681 tgttcttgtt ctttcgatga atctgccgta ctttcggaga tcgtggtact gctgctggaa
    25741 gcctgagctg aacttgtgct ttgagtagta gacgtagtag caccacaccc actcaacaaa
    25801 cttcctgcga ccaaaaacaa tgcaactaat tttttcacta aatttcctcc ttaagtaaaa
    25861 aacaaccgtt ttcaataaat gtccttttca attgtttact tacttgttat aataattgtg
    25921 ttatgaatag tgattgagga aacttaatct aatcccgttt tgatacacgg gattttttgc
    25981 tatagttctg ttattaagta tgcaataata aaatatgtac gttattttat tattagatta
    26041 ttgacattta tataataaac gattaaatga ttcatatcaa tagaatactt aggaaaaaaa
    26101 gagaaataat aacaggttta tgcactattg acggtgtgac aatatgatat ttgtatccgt
    26161 tttttttctt gtatgtagta tagtaaagta gatacaggag gagaaaatat gcagactttt
    26221 ttaacaaaag tttctcaaaa gaaaattaaa atgattcatt tactgcttga aacagaacgt
    26281 tggtgttcaa tagaagaact acaaaacgaa ttgaaggtat caagtaagtc tatcttgaat
    26341 tatctaacag atttagaaga attgttccaa aaatatcctg ataaggtcat tttgaagaat
    26401 gagaataatc ggcgcttttc tatggaaaag caggagaact tcccaatcta cattatttat
    26461 cttcatttct acagaaaatc atataattat catttgattg aattcatgta tcaacatcct
    26521 gagaaaaatt tggaagatta tgcagacgct caatatacga gtgtatccac agtttttcgt
    26581 tatgctaagt tattagtaaa gtactttgaa atgacggacg tcaaagaata gtagccgcca
    26641 gatagaagga aattagcagt tgaaaaagct gccctgcatc aagtaatacg accagccaaa
    26701 cggaaacagt gtctcatgtt tttgatttgg ctggtcttat taccgcagat gcaagctttt
    26761 gccacacctc tttcttgtca tttacagact caactatcaa tgctgcaagc tttcccttaa
    26821 tgctgtaaca ccagtaagag ttgtcaagac cagcagactg actaggtaaa taccgctcaa
    26881 aaacaagatt acggcagtta aactataatg atccatcaag aaaccgatga cgatagaaga
    26941 aaaaccacca acagctcgtc caaggttcat aatgaaatta ttcgccgtcg cgcggatttc
    27001 tgttggatac aaactactaa tcactgcgcc atatccacca tacatgccat tgataaagta
    27061 gccgactatc acagcggctg ctaccaaact ccactggcta tgggctaaca caagtagata
    27121 gacagataag gatgaacaaa ttaaaaacaa agaaaaggct agtttaggcc ccaatttatc
    27181 catgattatg ccaaaagtca tcatccccaa agacatgcct aaaatagtac tgaccatcca
    27241 caatgaagac ccagaaaccg aaagccctaa tcgattttgc ataattgaag gcagccaatt
    27301 cattaaccca aaatagccgg caatttggac agttaccatg atactaaggc cacatgtttg
    27361 ccaagcaaga tgttttgatt caaacagtct gctaagtttg gttttctttt cggcagattg
    27421 tacagatttg aattcctttg gttcagtcaa gccgcgacga atgaacaaga ccatcaacac
    27481 cggcagtaca cctacgacat acaacatctt ccaaccaaac gttggaatga tcacagcagc
    27541 taaaatcgca gccaaagccg caccgacctg cgcaccaata ccagcaaccg aagaagctcg
    27601 tccaatttgt ttttttgaaa aactttcgga aatcaaggac atacacgccc cgtattctcc
    27661 tcccgctcct acacctgcga taaagcgaaa gagataaacc aagtagatgt ttgaagcaaa
    27721 atacatcaat aaagaagcaa tcgaaaacag aaggaccgtt tgagaaaata ctttcacacg
    27781 tccatattta tcagctatga tgccaaaaaa aatacctcca atgagcatcc ccaaattagt
    27841 gatcgttgaa atgaaacccg cttgcgtacc actaatatga aaagaagtaa tcattgaaga
    27901 taatgaaaaa gctaaaaaca taatatccat attctctaaa gcaattcccg ccgatgtcga
    27961 taataataac tttcgctgat acgttgtcat ttttataccc cttttcctaa aatttcccta
    28021 aattgttaaa agtatatcca aaaaagaata aaaaaacaag acttttttat ttgaaataat
    28081 acacaaacaa ccgatataga taatccagct tagttttaat agaaatgagt attgacttgg
    28141 agtacactta aagttgtaga ataaataaaa tacataagta aagtggcaag aaattaagag
    28201 aaagagtgat cagatgaata tcaaacaagt cagtgaagaa aagggcattt cggccgatac
    28261 attgcgctat tatgaacgaa tcgggttgat tccaccagtt aatcggacaa aaggcggaat
    28321 ccgtgattat acagaagaag acctaaaatg ggtcgacttt acgatctgta tgcgaaatgc
    28381 cggattatcg atcgagtctt tgattgaata tattcgccta tctagcgaag gagaagcaac
    28441 tgtatttgag cggcgtaagc ttttgattga agaaagtgaa cttttgacga aaaaaattgc
    28501 tgaaatgcaa gaatgtttgg aacgtttaca aggaaaaatc aagaaatatg atcgtgtgtt
    28561 atctagaaat aaaatggaat gtatcaagta aaaaaatcaa ggagcatcgc tatggagtta
    28621 cgcttactaa aatatttttg gacaattgca gaagaaggaa caatctctca agccgcagaa
    28681 gttctacatc ttacacaacc aaccttaagc cgacaactga aggagttaga agaggaacta
    28741 ggaacagaac tgtttgtacg cgaaaaccgc aaaatgatct tgacagaagc aggttatttt
    28801 ctaaaaaagc cgagcagaag aaattttaga tttaacgaat caaaccactc aagaattcga
    28861 gaacagaaaa aaacaattat tcagcggccg atttacgatc ggctgtgtgg aagctgataa
    28921 ttcgaataca ttggcactat tactagaaaa gttgatccgt gattatccca gcgttcaatt
    28981 tcatatccac agcggaacaa gtgatgatat cacggagtga ctagaaaaaa gcctgttaga
    29041 tttagcgatt ttgctagatc cagtagatac agagaaatac gaaacgattt tgttaccaag
    29101 aaaggaaaaa tggggattgc tcattttaga aaattctgtt ttagcaaaaa aagacgtgat
    29161 aacaaaagaa gactttgcga atgtacctct tttgatctca aagcgtccag aaatacagcg
    29221 aatgtttgct gactggttag gcataaaaaa ggagcagttg ctgatcacag gaacgtacaa
    29281 tttgattttc aatgtatttg cgctaattga gaatcaggta ggtgctgctt tagtcataga
    29341 aggtgctgta agtcatcttt caaagcgagt gacattcgtt ccgcttgacc caatgattga
    29401 aacgaactgc atgctagtct ggaaaaagaa tcggatttta tcacctgtag tcaatgaatt
    29461 gattcaccgt ttcaaagaag cttttgaaca gtaataagta tctctaatcg aagaaatttc
    29521 aatacaaaag cgagacgtat gacagtcttg cttttgtatt ttttcatttt taaatcaaaa
    29581 aaaataaaga gatttacata acttgttaaa taagcgctta caaacataag aagaacggaa
    29641 atgaatcagc tataataacc gttgttctgg attgtaatca ttacaaagaa atgaagatta
    29701 taactttgga ggtgttcgaa atgaaagaaa aaaagcaaaa attcgttcaa aaaacatgcc
    29761 ctttattcaa tggttgggta tgattctgct gattgtatta gctttattga cgatcattcc
    29821 ttttttatgg atggtttctg cttcattcaa agcaaataat gaagtgttca cgattccgat
    29881 tcaatggatc cctaaaactt ggcatttaga gaattatctg gttatttggt ctcgtattcc
    29941 tttagtgaca ttttttaaga atacagcttt tttaagcgtt gtgatcacgt tgattcagtt
    30001 gtttacttct agttttgctg cttacgggtt ttctaaacta aattttaaag ggcgcgatat
    30061 tttattttta gcatatatcg gcacgattgc tgtcccttgg cagtcttata tgatcccgca
    30121 gttcattatg atgagaaaag tgggtttaac atatacgttg tggtcgttgg tgctattaca
    30181 agcgttcaat gcttttggtg tgttcttgtt gaagcaatac tacagtagta tcccagattc
    30241 tttatgtgag agtgcgcgga tcgatggctt atcagagtgg ggaatctatc gcaagatcat
    30301 tttacctctt acaaaaccgg cgattgccag tttgacgatc atcacattcg ttaatacatg
    30361 gaacgattat atggggccat tcatttattt gtcttctacg gaaaataaaa caatccagtt
    30421 agggttgaaa atgtttattg ggctatttga tgcagagtac gcattaatca tggctgcctc
    30481 agttgtttct gttcttcctg ttgcttttat atttttagcg atgcaaaaat attttgtaga
    30541 agggatcgct tcatcaggaa tgaaggaata ggaggtgatt atgaaagagc agcgttttga
    30601 caaaaatgtt tatatggaat tcttttattg gttgtatcaa tcattggcaa gtagcctttg
    30661 tttttggtgt gtcaatatct tgtatatagc agctgcgttc cttttgtcga tagatggaag
    30721 gaatgtcttt tcctttattt tttctttact ttttattggt ccaggttgca tcgtgatgat
    30781 aggtatgaca gaagaattag caaaagaaaa aagtttttca ccagtacgtt tgttcataaa
    30841 gacgtttcgc caattttggc taaagggatt tttttattgg ctatttgctt ggatagttag
    30901 tgtgataatg atttttgatt gtttcttttt tatacgtttt tcttatggaa aatggttgat
    30961 tccgttgttt gttttacttg cttgtttatc agtttccttt agcatcaact actggtattt
    31021 tcaggtgaga aatccagcgt cgaaaccaaa tcaagttcta cggatagcgt tctactatac
    31081 gttgaaaaaa tggtatgtga gtttactaga ttttttacta ttgacaagtt tgtttctctt
    31141 ctttttcgta aagccccaat ggtgtattct actaggtcct tctatcgtat ttggtttgat
    31201 ttacttcaac aatcgtaaat tgatgagaac tatggatctg tagactttat aaattgaaac
    31261 tttcaattaa gaatgacagt ttttatgtaa aaactgtcat tctttttcat tttttgaaac
    31321 atatggctaa acaaatgaaa taaaacgtgc tatacttttg aatgaaacag gtttagaaac
    31381 tgtatcaatt atttgtgatt atttctaact aataattaaa aataaagcat aaaaaggacg
    31441 atgttcaatg gacgaaaaaa tgaaacaagg ttcgaaatct gtagtagaaa gtttgattaa
    31501 tcatcatgtt gattatgtat tcggtatacc aggtgcaaaa atcgatggtg tatttaatga
    31561 attagaggat caaggtcctg aattaatcgt gactcgtcac gaacaaaatg ctgcatttat
    31621 ggcacaagca atcggacgta ttacaggaga gccaggagta gttatcgcaa caagtggtcc
    31681 tggtgctagt aacttagcta caggtttggt aacagcgaca gcagaaggtg acccagtttt
    31741 agcaattgcc ggacaagtaa aacgaagcga tctattgaag ttgacccatc aaagcatgga
    31801 taatgcagca ctgttccagc caatcacaaa atacagtgca gaaatccaag atccagaaac
    31861 aatttcagaa gtgattgcca atgcttatcg tatggctaag agttcaaaaa aaggcgcgag
    31921 ctttatcagt atcccgcaag atgtagtgga tgcacctgtc caagggaatg tcatcaagcc
    31981 attgagcgat cctaaattag gttctgcttc tgcagatgat atccgttacc tagctgaacg
    32041 cattagagaa gcaaaactac cggtactatt agtaggtatg cgaggatcaa gtgaaaaaga
    32101 aacgttagcc atcaggcaat tagttggaaa gacagcattg cctgttgtag agacattcca
    32161 agcagctgga gtgatctcac gtgaattaga agcgcatttc tttggtcgtg tgggtttatt
    32221 tagaaaccaa ccaggagata tgcttttgaa acgaagtgat ctagttattg ctattggcta
    32281 tgatccaatc gaatatgaag caagaaactg gaatgcggaa aaagatgcgc ggatcattgt
    32341 gatcgatgaa gcgccagctg aaatcgatcc ttttatgcag cctgaaagag aattgatcgg
    32401 tgatatctca gccactcttg atttattgac aggttcatta gagcctcaac aagtgtcaga
    32461 agatgcaaaa gaatatttag catctcttca agcagaatta acggagcggg acattgttca
    32521 atcaaaagga gaagcaggta tcttgcatcc tttagaagtt attaacacgc ttcagtcaaa
    32581 agtaacagat gatatgactg ttaccgtaga tgtaggtagt cattatattt ggatggcacg
    32641 ccactttaga agttatgaac ctcgtcattt gttattcagt aatggtatgc aaacgcttgg
    32701 tgttgcattg ccatgggcga tttctgctgc attggtgcgt ccaaatacac aaatcgtatc
    32761 agtttccggc gatgggggat ttttattctc tgcccaggat ttagaaacgg cggtacgtaa
    32821 aaaattgaat atcgttcatt tgatttggaa tgatggacac tacaatatgg tagagttcca
    32881 agaaaaaatg aaatatcagc gggcgtcagg tgtagacttt ggtccagttg attttgtgaa
    32941 atatgcagaa gcttttggcg ctaaaggaat ccgagctaca agtgtagagg aattagaaaa
    33001 agctttagaa gaaggatttg cgacagaagg acccgtaatt attgatattc cgattgatta
    33061 ccgtgataat gagaaattgg gagaaacaat cttaccagat caattctatt aaggaagtgt
    33121 cgacatgact gaaaaaatct tataccagca cgggacatta ggagctttga tggctggctt
    33181 gatggatgga acagaaacga ttgctcatat attagaaaaa ggaactctgg gattgggaac
    33241 attgcatggg cttgatggtg aagtcatctt tttagatggc gtagcttatc aaggaagatc
    33301 agatggctca gttgcccaat tagatggaag tgaacttacc ccctatgcag caattacaga
    33361 ttttacgcca gatactagtt tttctgtctc aaaaactgca gatggagaac aattgaaaaa
    33421 agacatatta gtaagagaag ccggggaaaa cctatttttg gcagtgaaga taactggatt
    33481 attcaaaaat atgcacatcc gtatcatgcc gaagcaagaa aaaccttaca gaagactagc
    33541 aaaaatttca gagtctcaac cagaattcca acaaagcaat gtccaaggaa cattagtcgg
    33601 gttctttacg cctgaattat tccaaggagt tgctgcagcg ggatttcact tgcattttat
    33661 cgatgataca catacatttg gcggacatgt aatggatttt gaagtggctg aaggaaaagt
    33721 tgagattagc cgcatctcgt cccttgtaca gcattttcca gttgatgatc cgacttttgt
    33781 tcacgaaacg attgattatg ctgatttagc agcagaaatc caacaagcag aatagaaaaa
    33841 gacagcgaga tgcgttcaag gtgattaacc tgacgtatct cgtttttttg aggaggaaac
    33901 ctagcgttta caaaaaaact gagctataat cttctccata gatcaaaaag gagcggtttt
    33961 tatcatgtgg acagaaaaac agaaaaaaga tgcatacgaa attttattat tacagcaaca
    34021 aggattatta gaagcagaag ataactggtt agcaaattta gcaaattcat ctgcattgtt
    34081 gaatgagact ttgcctgaga ctgtctttgc aggatattat ttattcgatg gtaatgaatt
    34141 gattttagga ccgttccaag ggcgagtttc ttgcacacgt atcaagatgg gaaaaggagt
    34201 ttgtggagaa tcagctgaaa aacaagaaac attgatcgtt gatgatgtca aaacccatgc
    34261 aaattacatt tcctgcgatt cagccgcaca atcagaaatc gtagtgccaa tggtaaaaga
    34321 aggtaattta atcggtgttt tagatatcga ttgcggagta acgaaagggt atgatgaaat
    34381 cgatcaagca tttctagaaa aatatgtaga tcttttatta gcaaatttta atataaatgc
    34441 tttttcattt attaataact aaaatcgtct attgaaacaa aaaagaagag aaatcaagaa
    34501 taaaaagagc cggaaagaca gtgggcattt ctgtttttcg gtttttttat ttttttataa
    34561 aatcacaagt gttattgatt ttcataatca tattttaatg gtattatcat tttaaataat
    34621 ggaaaaacat taaggatgga tgaaatatga ataacgaaac tatcatttta ggaaaaaaat
    34681 atatttgcca acctattggc ttgaaacaaa aagtggttgg agaagtaatc agtaaattag
    34741 aaaattgtgt tgtgatctgc gtagacgaat atcatgacgt cgatcgcaat gaaatattag
    34801 aaaaatgcgg aaaagtagtg gcaaaatata ctcacgttta tgagttggaa acaacagaat
    34861 atctatataa ctatagcaac aaatatgaac atactttagt ttcataaaga tttaaataaa
    34921 gtgccttcaa aagcgagaag accaagcttt tgaaggcact ttatttagtt ggcagtgatt
    34981 gttcatgatt tttgatttat tcaaaaggtt cgcctagaga atctgtctct gtaacgaggc
    35041 ctggtggaga aactcgataa aaacctatac tgccagaact gtcctatttt cggatagaca
    35101 aactagtagt tttaaaaaga tcatccgatc caatcatttc aaaaaatgca aaactgatat
    35161 caggatcatt tagctcaggc ttgttatcgt aaagcatttg taaaggttgc atttagttag
    35221 gagtaaacgc gaaactatca tcattatttg ctgaagaaga tgaagaagct aattcagagt
    35281 ttgtttgaaa taaaggagac gtgctgacag tttctttacc ctgttttttg cagaggtaga
    35341 aacttttata tcggaagttt ctaccggaga atttttctca tttgtagaaa ttgttgaatc
    35401 gatactattc gaaatttgcg tatctgaagg tatgttcttt gatgcagaag tcccacaagc
    35461 agtaagtaac aataaatttg ataaactgat aaacagaatt cttttcatta tgtacacact
    35521 ccttatagta tcgtttagca tacacctatg ctcagtatta ggcaaaatca atgcaaaact
    35581 atcaagtctt ttatagtatc tatta
//
LOCUS       DACDPS010000020        34963 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.020, whole genome shotgun sequence.
ACCESSION   DACDPS010000020 DACDPS010000000
VERSION     DACDPS010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34963
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.020"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(203..1177)
                     /locus_tag="IWJ07_001066"
     CDS             complement(203..1177)
                     /locus_tag="IWJ07_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAP9332659.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(1158..3041)
                     /locus_tag="IWJ07_001067"
     CDS             complement(1158..3041)
                     /locus_tag="IWJ07_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAP9332660.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(3059..5284)
                     /locus_tag="IWJ07_001068"
     CDS             complement(3059..5284)
                     /locus_tag="IWJ07_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP9332661.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEEKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            5399..6433
                     /locus_tag="IWJ07_001069"
     CDS             5399..6433
                     /locus_tag="IWJ07_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9332662.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(6500..7513)
                     /locus_tag="IWJ07_001070"
     CDS             complement(6500..7513)
                     /locus_tag="IWJ07_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9332663.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKHLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(7591..7779)
                     /locus_tag="IWJ07_001071"
     CDS             complement(7591..7779)
                     /locus_tag="IWJ07_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAP9332664.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(8219..8347)
                     /locus_tag="IWJ07_001072"
     CDS             complement(8219..8347)
                     /locus_tag="IWJ07_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP9332665.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(8350..9723)
                     /gene="rlmD"
                     /locus_tag="IWJ07_001073"
     CDS             complement(8350..9723)
                     /gene="rlmD"
                     /locus_tag="IWJ07_001073"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP9332666.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(9834..10889)
                     /locus_tag="IWJ07_001074"
     CDS             complement(9834..10889)
                     /locus_tag="IWJ07_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP9332667.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(10899..12329)
                     /gene="gatB"
                     /locus_tag="IWJ07_001075"
     CDS             complement(10899..12329)
                     /gene="gatB"
                     /locus_tag="IWJ07_001075"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAP9332668.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(12326..13798)
                     /gene="gatA"
                     /locus_tag="IWJ07_001076"
     CDS             complement(12326..13798)
                     /gene="gatA"
                     /locus_tag="IWJ07_001076"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAP9332669.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(13798..14103)
                     /gene="gatC"
                     /locus_tag="IWJ07_001077"
     CDS             complement(13798..14103)
                     /gene="gatC"
                     /locus_tag="IWJ07_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAP9332670.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(14209..16245)
                     /gene="ligA"
                     /locus_tag="IWJ07_001078"
     CDS             complement(14209..16245)
                     /gene="ligA"
                     /locus_tag="IWJ07_001078"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAP9332671.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(16262..18496)
                     /gene="pcrA"
                     /locus_tag="IWJ07_001079"
     CDS             complement(16262..18496)
                     /gene="pcrA"
                     /locus_tag="IWJ07_001079"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAP9332672.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            18651..20210
                     /locus_tag="IWJ07_001080"
     CDS             18651..20210
                     /locus_tag="IWJ07_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAP9332673.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(20321..22252)
                     /locus_tag="IWJ07_001081"
     CDS             complement(20321..22252)
                     /locus_tag="IWJ07_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP9332674.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(22270..23184)
                     /gene="pfkB"
                     /locus_tag="IWJ07_001082"
     CDS             complement(22270..23184)
                     /gene="pfkB"
                     /locus_tag="IWJ07_001082"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP9332675.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(23181..23933)
                     /locus_tag="IWJ07_001083"
     CDS             complement(23181..23933)
                     /locus_tag="IWJ07_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9332676.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(24092..24301)
                     /locus_tag="IWJ07_001084"
     CDS             complement(24092..24301)
                     /locus_tag="IWJ07_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332677.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(24312..25640)
                     /locus_tag="IWJ07_001085"
     CDS             complement(24312..25640)
                     /locus_tag="IWJ07_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332678.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            complement(26005..26592)
                     /locus_tag="IWJ07_001086"
     CDS             complement(26005..26592)
                     /locus_tag="IWJ07_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAP9332679.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(26675..27925)
                     /gene="clpX"
                     /locus_tag="IWJ07_001087"
     CDS             complement(26675..27925)
                     /gene="clpX"
                     /locus_tag="IWJ07_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAP9332680.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(28172..29461)
                     /locus_tag="IWJ07_001088"
     CDS             complement(28172..29461)
                     /locus_tag="IWJ07_001088"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAP9332681.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(29615..30547)
                     /locus_tag="IWJ07_001089"
     CDS             complement(29615..30547)
                     /locus_tag="IWJ07_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332682.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(30704..31363)
                     /locus_tag="IWJ07_001090"
     CDS             complement(30704..31363)
                     /locus_tag="IWJ07_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP9332683.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(31366..32391)
                     /gene="sppA"
                     /locus_tag="IWJ07_001091"
     CDS             complement(31366..32391)
                     /gene="sppA"
                     /locus_tag="IWJ07_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP9332684.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(32404..32694)
                     /locus_tag="IWJ07_001092"
     CDS             complement(32404..32694)
                     /locus_tag="IWJ07_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332685.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            33104..34909
                     /gene="glmS"
                     /locus_tag="IWJ07_001093"
     CDS             33104..34909
                     /gene="glmS"
                     /locus_tag="IWJ07_001093"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAP9332686.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
BASE COUNT         9568 a         7457 c         6266 g        11672 t
ORIGIN      
        1 gactgtcaat catttttatg aaactttttt taattttctg atagttaact ttgtttttca
       61 gaaaaataac aattggtttt attgaagaaa atagtcatga acaaaagtat ctgagaactt
      121 ttttactctc ttgttcatga catatagaat aaagcagctt tgtcttgtaa actccgtttt
      181 tcaaataata atctaaagat tgctactgaa taataaagcc gatttcatgg tcctcttcgc
      241 tgctgattcg gtaaggtgac tcaacatccg ctccccagct attgataccc ccgactcctc
      301 ttacttttgc aaaaatcgta agaaccgttc gtctttctgg cggtagttct tctatatgtg
      361 ttgcctgttc cagttcttct gctttgaatg gtaaagcgga aaaagcaaat ggcgtatcct
      421 gctgttcaaa tcgcaacgaa aaatcttctt gatctttatc cgtattatcc aacgtttgat
      481 ttcttgttag ttctacccat ttcgtttcca tatgcatgcc gcattcttgg ggaacaaggt
      541 atggggaaac ggggagtcct gccactttat aaataccttc gacaccacca gccatccgat
      601 ccggatatgt ttcccctgat aggccttcat actcaaaaaa ttttgctttc gttggcataa
      661 tgaaccttaa acccaatgcg ggtaattccg gtaatccttt ttgtccataa taatgcatat
      721 tgacaagcat tttaccctct ccagcgattt ggtaggaaat tgtcacttgg gtttttggta
      781 ctgtgttgat ttccagtgta aaaatgattt caaccgtttc agcgtattca ttacttgaat
      841 attgattatt tactggagca atcggcaaat cgatcttttt attgtccacc agtacttcta
      901 tttctttgac ttttggatac aatccggctc ctaaccacat accggattta aatccaaatc
      961 cgctacctct gtcattatcg gtcgttgctc gccaaaaaac tggaagtgtt tcccggtata
     1021 gccattcttt tccgttttta ttgagcgatt ccagtccgcc tcttgtataa gaaaaaatat
     1081 aatgcgtatt ccccgatttg acacctaatg tacagtcgcc gaagacgatt gcaacttttt
     1141 ccgtatccat atgactttta tttactgtat ttttcataat aatatcttac ctcctgcatg
     1201 gcgggttttt cttgtccatc tgcaaaaacg atcccattcc ctgagaattc atagtcactt
     1261 ggtttatcgt caaagtcacc gccatagcgc atcacttctc tgcctgtgat tggatcagtc
     1321 acttggatcg cctgatcttt aaaatcccat atatagcctc cttggtacat ttcataacga
     1381 tcaagcaacg tcatgtaaga atccattcca cctaacgaat tccccatgtc atgcatatat
     1441 tcacataaga tgaacggttt ttccgcttta ttttcaaggt agtcaatgat ctcttctggt
     1501 ggcgcataca tgcagctttt tacatctgaa atcgaatctt cgaatgctgg atttggaaaa
     1561 ttcccttcat aatggacaag tcgatcgggg tctacttttt tgaagtaatc gttcatttgt
     1621 cgaatgtttt cttccgcata agattcattt ccaagagacc aaaaaagaat acttggatgg
     1681 ttttttaatg tttcgaaatt cgtttttgct cgatcaagaa cagccgcctg ccactgaggt
     1741 actgcacctg gtacgttgta agaaggctca aatgcgccca ttttttgcca tgagccatgg
     1801 gattcgaggt tcgtttccgc catcacatgg attcctgctt ggtcgcataa ataataccaa
     1861 gtgatctggt tggggtaatg agatgtccgt acgccgttga tctttgcatt ttttatgata
     1921 gccaaatctt tttccatatc ttctatagtg atggcgcgtc cttttttcgg atgccactca
     1981 tgtcgattta ccccgttgat aatcatccgt tttccattga agtagatgat tttatctttg
     2041 atttcgattt tacgaaaacc gaaggaatag tcaatatact ccgttactct tttttcttcg
     2101 tctatcgtct ccaattcaag cttatataaa ttgggctgaa aatgccccca cagtgccaca
     2161 ttttcaaatc catcgatgcg aatcgtcatt tgctctttta acggatattg ctgttgatag
     2221 atttccttcc catcaggatc tagtatcgct acattcagct gactgttttt ggctacttgc
     2281 tcaaaagaca aatctacttc aagtttgcca ttttgacatt tgtcatctaa gcgaggtttg
     2341 atccaaagat cttctaaatg gttttgtcct cgagcaacta agaagacatc tctgaagatc
     2401 ccaaaaaatc ggaaaaagtc ctgatcttcc aagaatgcag ctgtacttct tttgtaaacc
     2461 tccacagcta gaatattttc ttcttccaaa taaggtgtta aatcaaattc agacggagtg
     2521 aacgaatctt cggcataccc gataaaatga ccattcaacc aaataaacat tgcttgttct
     2581 acaccagcaa aatgaatcga gatttgtttt ccctttagac cttcttctaa tcgaaaagtc
     2641 gtcaaatacg aaccaaccgg attgtcttta gcagctgaaa agatcccttc cgcctctttc
     2701 tcagggcttg tcgtgaaagg gggacggcga aatacatgtc cctcccacgg atacatcgta
     2761 ttgatgtaat ggatcttgtc ataatttgct aattcaatat gactaggaac agtgatttca
     2821 tcaaaagaac aggcatcaaa atctttttta taaaatgttt ttggtcgtac cgaaggattt
     2881 ggcgaataac aaaaacgcca tgatccattt aaagactgtt taagggagga tgtggtttct
     2941 acttcttccc ttgttgcata acaaacatga tcgctatgtg catttgattg gttcactcta
     3001 aaaacttgtg gatcatctaa ccattttata tttgctttca tacttttttc cttctctctc
     3061 ataacttttt gaaatgaagc agttctgttt ggaaatcacc ttgaaaatcg gggaagtaaa
     3121 gtccaactgt catcaattcg gcccccgaaa atatccgctt cgaggtctgt tcctgataaa
     3181 gtgcctcatc atctaacccc atcagatata cttcatggaa cgctggttga gctgaagcta
     3241 gtatcctccc taaaaacaca atagcttcat cttgtttagg agacacaaac atccatgcgg
     3301 caatattttc ttcaaaagga ctcgccaaac gataataatg gccatactgg atcaccggac
     3361 gaatcgtttg ataggatata atttggtctt ttacttgctt ttcttcttca ggagacagct
     3421 ttgtcaaatc cagttcataa ccaaaaacag cgctcatagc tgtttctcct cttgttttca
     3481 acgaagtaga tctgtcggtc tgatgatttg gtacagctga tacatgagct gtgatggacg
     3541 aaatcggata agcaaggctt gttccatatt ggattttcat acgctccact gcatccgtgt
     3601 tatcacttgt ccatgactgc ggcatataat aaataaaccc ggcatcaaac cgtccacctc
     3661 caccagaaca gccttcccat aagatttccg gataatcagt tgtcaaatgt tcaagtagtt
     3721 cataaagccc gagcatataa cgatgcgcta cttctccttg tctctgtggg tcaagggtaa
     3781 tcgaatagac atctgaaagc gaacggttca tatcccattt gatgtagtca agaggaatcg
     3841 tatctaaaat ctttctcatt tgtttctcaa tcgtctgccg aacatctaac cttgtaaaat
     3901 ccagtacatg ttgtgatctg gaagcagaag gcattctacc tggctcctgc ataagaaaat
     3961 caggatgtgt tcgatacagt tcagaatcaa ccgagatcat ttcaggttca aaccataaac
     4021 caaatttcaa tcctttgcta tgagcataat ctgctatttc acgtaatcca tttgtcaact
     4081 tgccttcata ctcgaaccag tctcctaatg aagaattgtc cgcatctctt tttccaaacc
     4141 agccatcatc taatacgaac atttcgatgc ctagctcctt tgcttcatca atgatcgcct
     4201 tgattttttc actattgaag tcaaagtaag tggcttccca gttattgacc agaatcggcc
     4261 gttcagcaaa ttgatgacga cctcttgcga ttctttcacg tagcaactca tggtggatct
     4321 gactcatttt gttcaatccc tgacttgagt aagaaaggat catttcaggt gtttgaaaag
     4381 actggcctgc attcaattcc cacgaaaaac gatggctgtt gatcccaccg acgactctca
     4441 attgattgat ctgatctttt tcaagttcga aagaatggtt gccggaataa acaaattgaa
     4501 gtccgatcgc ttctccagta ttttctgtcg tatgatgatg aactaaagca ataaagttat
     4561 tcatatggtg actgctcgtt cctcgacggc tttcaaatac ttttgttcca gataaaacag
     4621 attgccgtga aatctgtctt tcattgacat gtgctcctgg caaagcaatg acttcatcaa
     4681 atcgcctagt atgggcaaag tctagctgga aactagccgc tttttctagt ttgattgttt
     4741 ctccggtttc atttcttatc tttacagatc gtgtgatcac tgctctatca gtaaatatgg
     4801 tatagaacaa atcgaaataa atcgctgttt ctctatcctt aagtgaaatc accaacgttt
     4861 gtgcctctga cttgtccatc acataagttt gaggcaagcc ttcgatctct ggcttcccat
     4921 ctgtaatata atggctgtca tatctcagat ccagtaagtc tgatccattc ttgcgctgaa
     4981 tgatcgatgc gggcgttcga aagtccatgc tgccatgaga agagtattct tgcggtagtg
     5041 tatctttcga cagagaccgg tcaggattta agggaacatt tcctgaaaat ccacgatctc
     5101 ttcggggata atttttattg tctttgtact gcttgactgg tttgccaaaa taaatatgcg
     5161 ccaatacatt gctttcttct atctttatca gataagaaag aaatgtattt cgtaaatgga
     5221 agactcggct cttttcatca aataagatga aatttttatc tgtttttcta tgcgtaatat
     5281 ccatcatttc actcccaaca aaacggaaac cgtttacttt tttttataat tcctgtatac
     5341 tgtttactaa acaattttgt caataaaaat tttttatttt tttgaggagt gtgtaaaaat
     5401 ggttggaatt agagatgtcg cacgaaaggc aaatgtctct cctgctacag tttcccgtgt
     5461 gttgaataac gatcagacga tccatgtggc tcagactact cggcaacgga tagtagaggc
     5521 agctgctgcg ttggattatg acatcagtac tcgtaaatat atcaaaaaga gaatgccttc
     5581 gattggtgtg atctctacga tcggcaaagc gagcgaagaa caggatatct attacaaaca
     5641 tttgcgagta gggatggaag aagaagctcg acgtctgcat ttagggatga atcgaatcta
     5701 taatctatct gataatccta aacaatggga agatctagat cagttaggtg cggtgatcgt
     5761 tgttgggact gttacgaaag aatcgattac cgccttgtta aaacaaaaca tgcaaatagt
     5821 cgtcgtggat aaccctgaca tccagcaaga ggtcgatatg gtgtatgcag atcttgaacg
     5881 gatgactaaa accgtactgg aattgttctt agaaaatggt cataaaagaa tagcctatat
     5941 cgggggatat aaagtcgata tggatgaatt cggtgggaag cagatttcta taaatgaaaa
     6001 gaggatgcgt gcctatcagc attttatgat cgatcatcac ttagaaaaat atatgtctta
     6061 tcatctaggt ggctggacag aaaaacaagg tgaggtactt gcgactaatc tattagaaaa
     6121 tgaatcccct cttcctactg ccattcttgt cgggagcgat ccaatggcaa ttggtgtcta
     6181 tcgggcattg aaacaaaaaa atatccaaat tggagaagag attgttgtag caagctttga
     6241 taatatcgag acaatcgcaa acctctcccc tggcttgacc agtgttcaaa tcaatgcgaa
     6301 atcaattggg caagcagctg ttcgaatggc attggagcga attgacggta tacgggacga
     6361 accaatcatt ttgacttatc ctgcacgatt gattgttagg gaaagtttta tcccaagaac
     6421 aaaaaggtat tgaaagagag gttgtgaaaa gccgcttcgt ttcttcatac tcaaaacgaa
     6481 acggcttttt cacaacctct tacttatact tattcacaaa atccaatgtc tgctggtaac
     6541 aattttgcga ttcttttagc gtgtaatcaa attggtattc atgggtaatc tctttttttg
     6601 tatctgtata aaacaaggat tgcacgggta cctttaattc tgtcaaccga ttttctaatg
     6661 cttgaccttg ctcctggaat gagtatgcat ttccgtcagt tatatacgtt ggcggaaatt
     6721 ccttagaaac atggtcagcc acactagctt ctttcaattg ctcactcgtt ttccaatctt
     6781 ttgttccaat caacgaccac gcaacggttt tgacgaaaaa cttcataaat cgatcatccg
     6841 aatgctgctg tgctgttagc ttcaaatcaa caggtccgca ataagaaatg gttcctttca
     6901 gactatcttt tggaagtaga tgtttgattt tcatttgttt agcatacgca tcattcgttt
     6961 gtatagctgc atattgcaat gcgatttgtg aacctgcact atctccacca aaaaataatt
     7021 gttccatgtc tagcaatttg tcgtcttttt cttcagctaa taaatctttg atcagttcat
     7081 tcacttgaag aacttgatta gggtattctg aatcaggcgc taattcataa ttcatcgcaa
     7141 ccactgcgat gttggcgtct gaaactaatt ttgtcgcaaa ttctttgaca cccgatttat
     7201 ctccgccgac gaatcctcca ccatgtaccc aaaatagaac aggaacaggc cccttgctgt
     7261 tttttggata gtagatgtca taagtattct ctttatactt ggactcatac gtcaaatctg
     7321 tttttttcgc cacgtttttt tctgcttgat cgaacgtttt tttatcagtg atttgaacag
     7381 tatttgaaaa catatggttg atcaagaatg ctccaggtct gggggaaagc tggaaggcaa
     7441 gaaagattgc aaacgcgatc ccaagaatga ttccgaagaa ccatccgata aatttaagaa
     7501 ttttcttttt cataaggctc cctcagtagt tagtattaag cattctgatt ataccatagt
     7561 gttatttcct atcaagctct cctagatgat ttaatgattt tccccaaact tcttctgcaa
     7621 tgcttttgac aaaagaaatt ttttccattg ttcttcaaac aaaacatttt cttcttctgt
     7681 tgaagcaaat ccacattgag gacttaagca caaacgatcc aaaggcagat atcgtgtcgc
     7741 ttcgtagatc cggtcaatca cttgctggtg ctcttccatt tcgccatttt tcgttacagt
     7801 caaaatctaa gacaactaat ttatcttcag agactttctc taaaagaaca aaactcccag
     7861 cacgttcact atcgtattct aaataatagg cacggacatt ttcttgagta aaataatggg
     7921 tctgctacgt cgtcatcccc cctgatgccg ccaaaatcga ataataattt ccgcgtcaaa
     7981 catgtatatt tagtgttgaa tcatttgaga atatgaaaat aaaaatcttt ttgatcaatc
     8041 agttaatcaa attgaaataa cgaaaaatct gagacagaat gacgttcatt ctgacccaga
     8101 tttgattttg cacaactcaa cagtccttat tggcaaatcg ccaaaataat gtagactaac
     8161 taaaaagact aagaaacaat aacaaaagtt aacacttctt agtctttctt tagcacgtct
     8221 atttattaaa gattttaggg ccttttctca tgtttttacg atcattctta ggtgtttcca
     8281 tttgtttgga tcggcctcca ccttgtttct ttttgatcaa ttctttcatt ttttcttttt
     8341 ggtccatttt tattctcctt taacgaatga agcgactaat tcgatatgat gggtttgcgg
     8401 gaaaagatct acaggctgaa ctttcttcac ggcataacct gcttctgcaa ataacttagc
     8461 atctcttgca aaagttgccg gattacatga gatatagacg attcgttctg gtgacatttc
     8521 gattgctgat tcaataaaat gatgatctaa tccttttcgt ggagggtcaa ctacgatcac
     8581 agatggtttg atcccttttt cttgccattt tttcataact ttttctgctt ttccgacttc
     8641 ataatgaaca ttttcgattt tattatttaa agcattgaac tgtgcatctt caattgcttc
     8701 tggaataact tccatcccat acaccttatc cactctgtct gctaaagaca agccaatcgt
     8761 acctattcct gaataagcgt cgatcacaat gtctttttta tctaactcgg caaactcgat
     8821 ggctgtttgg tacagatact ctgcttgttg agtatttact tgataaaatg actttgctga
     8881 gattcgatag gtttttccta atagctgatc ttcgatatag tctctgccat ataaaacttt
     8941 ttctttgtct cctaaaatca catttgtttt ttcttcattg atattttgga tgaccgaaac
     9001 cacttctggc aattcttcat gaatagcttc agcaatttcc ttggatttaa agaatttttc
     9061 tttacgagta actaaaacga ccatcatttc atgggaataa tgaccacgac ggataatgat
     9121 gtgacgaagc tgtccttcat ttttttcttc attatatcct cgtacttgga accgttgcaa
     9181 gatatctctg actactttga ttgcttgatc gatgacagga tcttggatat aaaaatcttc
     9241 gatagggact agatcatggc tattcttttt gtaaaaaccg gtggctaact gtccatcgat
     9301 acggcgaaca gggatctgtg ctttattacg gtagccgaat ggttctgcca ttccgaccgt
     9361 tggaagtaca ggaacttctg gcatcttagc aatcttcgcc attacatgct ctacttgttt
     9421 ttgtttgaag acaagctgtt gttcatactt caaatgggct aaaggtgcga tacctatgcg
     9481 aagtaatgca ttgttttcta caggctgacg atcaggtgat tgtgtaataa atgtttcgac
     9541 ttttccatat ccaaacttat tgcctgcttt tactactagt atttctactt cttctccagg
     9601 gagtgcattt tcgataaata atggataacc atcgattttt gctaccccca ttccttcata
     9661 actcaagtcg acgatttcga ctgtatatcg ttcgtttttc tttactattt gctgttctgt
     9721 catattttgc tcctttaagc ctatttttca atcctactga actctactat aaatcagcta
     9781 attacacaac taaaaccggc actgacctta ctacgttcag caccggcttt tgattaattc
     9841 ttttgattag gtgtttcgtc tgctattttt ccgtcaccgt cgatatcttc atccgttaaa
     9901 cgttcgactt cttttacgaa ttctttgctg acaacctcta attcttcttc atctgtacct
     9961 gtgattgctt catcaggaat ctgatcggta ttggcaaaga actcaatgtg ttgatgaaga
    10021 ttttggaaat gcatcggtgc ttccccgccg tattcaccat ctaagttgat cattactggt
    10081 cgattgatat catcttctgt ttcaacgaac aaactgcttg ttttggtata gattattctt
    10141 ggatcatcga catgtcgacc gccattcagc atgagtgctg caatatgcaa gatctcaaag
    10201 atatttgctg tcttcacaat gattaaagaa aacttcccat catccaattt ggcatctggc
    10261 gcgatctgtt cgaatcctcc aacagagttc gtcagcccta ggaaaaacat cgaagcattt
    10321 ccgatatatt ccccttcatc atacttcaag cgcattttga ttggctttac tcgagggagc
    10381 atctctgctc ctttcgcaag ataagcaaga taaccaaaaa tactctttag ttctgatgga
    10441 acttcataag tcaattctgt caaatagcca cctgcagcga tattgatgaa gtaactgtta
    10501 tcagaaaggc cgatgtccat ttttacggtt tggtgttttt ttatcacttc tgctgctgct
    10561 ttaatattat ctctaggaat ttttaatgct cgagcatagt cgttcgttgt accagctggg
    10621 atgatggcca ttttaggacg gtgttcaagc ggagcgattc cattaaccac ttcgttgatt
    10681 gtgccatcgc caccagctgc cactactaga tcaaaaccta gttctgcgac tctacatgct
    10741 tcattcttgg ctgaatctgg ttctggtgta gttgcatagg cgctggcttc aaatcctgct
    10801 tcttctaatg cctgtaggat atccgccaaa ttttttttca ataattcttt tcccgaggtg
    10861 gggttataaa ttacgcgtgc ttttttcatt ttcagcctct atcgtttcgc taattcatct
    10921 ttaagtagct tattcacgac accaggattt gcttgtcctt tagttgcttt catgatttga
    10981 ccgactagga agccaaccgc acgatctttt ccatttttga aatcatcgac agattgttgg
    11041 ttgttatcca aaatttcatt aatgattggc agtagtttgt tcggatcaga taattgcacc
    11101 caaccatttt tttcaactac ttcttgggct tctccgccat ttgtgatcaa ttcacggaag
    11161 acttttttgg cgatttttga gctgatcgtt ccatcggcga tcaaacgaat cattcctgct
    11221 aagttctcag gtgtcaattt tgtctcagct aattccagtt tctcactgtt caagtaagca
    11281 gaaacttcac ccatcaacca gttagatgct tgcttggcat ctgcaccatt ttccaaaaca
    11341 gcttcaaaaa aatcagacat ttcttttgta agtgtcaata ccattgcgtc atactctgga
    11401 agtccaaact cacggatata gcgttcgcga cgagaagctg gcatctcagg caaactagct
    11461 tttacttttt gaatccattc gtcatcgatc acgaatttcg ggatatcagg ctctgggaag
    11521 taacgataat cactagaccc ttctttgaca cgcatcaaaa ctgttttatt cgttgtttcg
    11581 tcaaaacggc gtgtttcttg ttggatttct cctcctgaaa gaagaacttt tgcttgacgt
    11641 ttttcttcaa aagctaaacc ttttttcacg aaagataaag agttcaagtt tttcaattct
    11701 gctttcgtac cgaactcttc ttgtccataa gggcgaagag aaatattggc atcacagcgc
    11761 atagagcctt cttccatttt cacatcgctg acttctgtaa attggataat tgatcgcaat
    11821 gcttctagat aagcgtatgc ttcttctggt gaacgcatat cagcttcaga aacgatttcg
    11881 atcaaaggcg tgccttgtcg gttcaaatca acgtaagaat aaccgccaat accatgcatg
    11941 ttttttccag catcttcttc taaatggaca cgttcgatcc ggattttttt ctttttacct
    12001 tctacttcga tttcgatcca tccgtcatga ccgattggtt gatcaaattg ggaaatttga
    12061 tatgcttttg gattatctgg gtaaaagtaa tttttccggt caaaatgagt atctttagag
    12121 atttcacagt ttaatgccaa agctgctttc atcccgaatt ccagcgcagc tttattcatt
    12181 actggcagca cgcctggata accccagtcg atcacgtttg tattgctgtt tggttctgca
    12241 ccaaaatgag ctggcgctgg agagaaaatt tttgagttgg tttttaactc gacgtggact
    12301 tctagtccaa tgactgtttc aaaattcatt cgtctttccc tcctaaaata gctggttgtt
    12361 gtttatggaa ttctgttgct tgttcaaacg catatgccgc ttgatacatc gttttttcat
    12421 caaaataatt accgatgatc tgcaatccaa ctggtaatcc ttctgataaa cctgccggta
    12481 cagacatacc tggaagtcca gccaagttga cagggatcgt taagatgtca ctcatgtaca
    12541 ttgtgattgg atcgttaatg ttctcaccga ttccgaatgc aactgttggt gaagatggcc
    12601 cgatgatcaa gtcataatct gcaaagactt tttcaaaatc tcgtttgatc aatgtacgga
    12661 cttgtcctgc ttttttgaag taagcgtcat aataacccgc gcttaaggag aatgtaccta
    12721 acatgatacg gcgttttact tcttcaccga atccttctga acgtgagttg acatatacat
    12781 catctaatga tttcacattt tcagagcgat aaccgtagcg gataccatcg aatcgttgca
    12841 aattggaact tgcttctgat gaggcaatga tataataaac agccacaccg tattttgaat
    12901 gaggcaagct gacttcttcc acaacagcac ctaatgaacg gaatgtttct gctgctttca
    12961 atacagcttc ttttacgcca ggggccacac cttcgcctaa gtattcttta ggtaaagcaa
    13021 tcttcattcc tttgatgtcg cctgtcagtc cctctgcaaa atcaggaact gaaacgcctg
    13081 aagaagtacc gtctttttcg tcataaccgc tgatagctgt caatgctaat gcattatctt
    13141 tcaccgtacg ggtcattggt ccgatttggt caagagatga agcaaaggca atcaaaccaa
    13201 aacgtgagac acggccataa gtaggtttca tccctacgat gccgttaaaa gctgctggtt
    13261 ggcggatact tcccccagta tcactaccta aagaaactgg aatttgtcca gctgcaactg
    13321 ctgctgcaga accaccagaa gagccaccag gtacttttgt tgggtcccaa gcattttttg
    13381 ttttcttgaa ataagaagtt tctgtacttc cacccatagc aaattcatcc atgtttagct
    13441 taccaacagg gatcatatct gcttgataga ttttatccat gaccgttgca tcataaattg
    13501 gttcaaaatt gtaaaggatc ttagatgcag cggttgtcaa gatatctttt gttacgatat
    13561 tatccttgat accaattgga ataccagcaa ggacgttgct ttcatcaatc cctttttcat
    13621 ctaaagcttt tgcctgttcc aatgcttttt cttcgttcaa tgtaatgaat gcctcgacct
    13681 caggctctgt ttctttgatt cgttcaaatg ttgtttttgt caaatcttgt acagtgattt
    13741 ctttcgatac aagcatatga tgcagttctt ctagagaacg attgtgtaat tctgtcatta
    13801 tgcgccagcc tccccattat cgatgattgc gggtactttg ataaagccgt cttctgtttc
    13861 cggcacattt ttcaataatt catcgcggtt ccatccagct tgcgcccgat cttgtcgcat
    13921 cacgtttact gtttcaacca cattcgacgt aaatggaaca ccttccgtat ctacttcttc
    13981 cagcatttca accatatcga tgattttacc taattggtcg gtaaaacttg caagttcttc
    14041 atctgcgaac gcaagttttg ctaatttggc aacgtgtttc acttgttctt cactaatcgc
    14101 catactaccc cttctttcta aacgagttaa aaactttgct ctctatttta acacaagagt
    14161 gccgtgtctg aaattgttta ttgaaatttg tctgaaaaaa ctcatttttc attcttctac
    14221 tgagcgactt ttagccaatg catctgccat ttgatcttct gtccaaactt cgattcctaa
    14281 ttcttgtgct ttggtcagct tgctgccagc atcttctcct gctaccacga tatccgtttt
    14341 tttcgatacg ctaccagtca ctttcccgcc aagattttcg atcgtctctt ttgcttcttc
    14401 acgagtaaat cgtgttagtt taccagtaag gactactgtt ttttctttaa aaggagactc
    14461 gacttcttgc aattgggcat ttcgaagccc tttgtattcg aaattgacgc ctgcttgttt
    14521 taattcattc atcaattcat ggacttcttc gttactgaaa taagtgacta cactgtcggc
    14581 aatgatatca ccgatcgtat caagcgcaat aatcgactca tagtcacttt tactcaatgt
    14641 ttctagatca ccgaaatgtt ctgctaaaat ttttgctgct ttcgctccga catgtcggat
    14701 tcctaacccg aaaatcaacc gctcgaccga attatctttg ctgttgtcga ttgctgtcaa
    14761 aatattattt gctgattttt cttttatttt gtccaacgtc aataactctt cttctgtcaa
    14821 tttgtaaaga tcagcaacat ctgctactag ttttttatca tacatctgtt ctagtacgcg
    14881 cggccccaac ccatcgatat tcatcgcatt tcttgaaaca aagtggttca aaccttcttt
    14941 catttgagca gggcatttag gattgatgca ccgtaaggct acttcttcat ccaaatggac
    15001 aagttcactt ccacaaactg gacaatgtgt aggcaattgg tattcttcgc tgtctttagg
    15061 tcgtttatct aacacgactt gcgaaacttc tgggatgatg tctcccgctt tataaatcag
    15121 aactgtatct ttcaagcgga tatccttcaa cttaatataa tcaccgttat gcaggctggc
    15181 acggcttact gtcgttcctg ctactcggac tggtgtcatg atagctgttg gcgttactac
    15241 ccctgttcga ccgactgtcc attcgatgtt ttcgattagc gtttcaactt cttctggcgg
    15301 aaacttgtaa gctgctgccc atctcggtgc tttgacagta aaaccaagtt gatcttgtag
    15361 cgagaattca ttcactttga tgacgattcc atcgatttca tagggcaaat ccacacgttt
    15421 gtcatgatat tcttcaatgt atgaccacac ttcatcaatc gaatgacata gacgtttttc
    15481 ccgattcgtg tggaatccaa ttttttctag ctcttctagc gcatcatact gggtttttgc
    15541 ttgcatagga ccgaaatcag ctactgtgta taaaaaagtg tccagatttc ttttcgctgt
    15601 gattttagaa tctaactgac gcaaagaacc agctgcggcg ttccgaggat tcgcaaagat
    15661 atcttttccc tctgcttctc tgtcttggtt caattggaca aatgaccgtt ttggcatgaa
    15721 acattcgcct cttacttcga tgttcattgg ttcttttaat ttgattggga ccgaccggac
    15781 agtttttagg ttttcagtga tgttttctcc tacagttcca tccccgcgag tcgcacctct
    15841 cacgaaattt ccatcttcat aacgcaaaga aacggaaagt ccatctattt ttagttcaca
    15901 gcaatagtca actacacgtc ccactgcttt ttgtacacgt tgatcaaaag ctatcagctc
    15961 ttctttgctg aaaacgtcgt tcaaactgta aagcggaata tcatgagtga ctttttcaaa
    16021 accttctaat acttttccgc ctactcgttg agtgggagaa tcagacgtga tcaaatcagg
    16081 atattgttcc tcaattgcta ctaattctgc gtattcttta tcataaacat agtcttccac
    16141 tgttggttta tcttctacgt agtattcgcg agaccattgg ttcagtcttg tacgaagttc
    16201 ttctgctcgt ttagcagctt cgtcaatcgt catttcatgt tccatcattt accctccact
    16261 actaagcttt ttcaattggc gcaaaggcag ccaaaagacg ttttactcct tgacttggga
    16321 aagccacatc tagttctaga tccttcgacg tcccgcttac tcggacgact gttcctagac
    16381 cccatttttt gtgtttgact ttatctccag gtttccagct gttattttcc cctccagtag
    16441 cacttttaga gggaacaacc gctgtctctg gctgtttata gctatatgct gtttttttag
    16501 cgaatacagg tgttttttta ggcgtaggac gcatgccttc gatttccagt aactcctgat
    16561 cgatttcttc tacaaaacga gaaggtcggt tatattgcgt ccgtccatat aatgttctgg
    16621 agaaagcatt tgttaaatac aacgcttctt ctgctcgcgt gatccccaca tatgcgagac
    16681 ggcgttcttc ttccagttcg ctttcttcca tcaatgctcg agataaagga aaaaccccct
    16741 cttctagacc aatcaggaat acaactggga attctaatcc tttggctgca tgcaaagtca
    16801 tcaatgtcac ttgggaagca tcttcttcta aattgtcaat atcggaaaca agtgccagat
    16861 catttaagaa gaccgttagt ttttcttctg gtgcatcagc gtcttcttca ttttgctgtt
    16921 cgaattgttt gtcgaattct tgtgtgaccg ttaaaaattc ctccaagttt tcgatgcgtg
    16981 cttgtgcttc taatgtattt tggattttta agtcttctag atatccgcta cgatccaaaa
    17041 cctctttcgt caattctgtc actgtcaagt atgggatcat ctgagtgact tcttggatca
    17101 tctctccaaa tgcacctaat tgctgtcctg cttttccacc gatatttgcc agatccacat
    17161 tttgcgctgc ttcaagcagt ggccattcat gaagagaagc aaaactgcgt agtttttcga
    17221 tagatcctgg tccgatgcct cgtttgggcg aattaacgat acgctcaaaa ctgattgaat
    17281 cttgaggatt tgccagcaca ttcaaatacg ctaaaatatc tttgatttct ttacgatcgt
    17341 agaacttgtg ccctccaacc attttataag gaatatttgc tttcaacaga gtttcttcca
    17401 ttacacgaga ttgtgcgttc gtccggtaca agatggcaaa gtcgccgtaa ttccgatgat
    17461 tgcttcgaat ctcttcttgc atccgatcta cgataaaacg ggtttcgtct cgttcattat
    17521 ctgcacggta gtaagtgatc ttgtttcctt ctttgttttc tgtccacaag ttttttggtt
    17581 ttcgattgct attgttttca atgacttggt ttgctgcgct aaggatgttt ttcgtggagc
    17641 gatagttttg ttctagcaaa attactgcag catctggata atctttttca aaatccaaga
    17701 tgttctgcat atctgcaccg cgccagccgt aaatactttg atctgcatct cccacgacgc
    17761 acaggttcct aaatctacct gccaataaat tgactagcgt atactgtgcg tgatttgtat
    17821 cttggtactc atctacatga atataatgga atttgttttg ataatatgtg agtgaatcag
    17881 gatgttcttc aaataatcga atcgtattca tgatcaggtc gtcaaagtcc atacattgat
    17941 tatattgaag ctctttttgg taagcagcat aacattttgc cgcaatctct tcgtaataac
    18001 tgccttgcat ttttgcaaaa tcctgagggg tctgcaggct gttttttgct tgagagattg
    18061 ttcctaatat acttcgagga tcatattttt tcggatcaat gttcagttct tttagtatcc
    18121 gtttcatcaa ggtcagctgt tcagaagaat cgatgattgt aaagttccgg ttatacccga
    18181 taaaatcaac atctcttctt aaaatacgca cacacatcga gtgaaaagtc gatacccaga
    18241 catcttcgcc tccagatgcg agaatcgcat tgacacgttc tttcatttct tttgcagcct
    18301 tattggtaaa agtaatcgct aggatgttcc aaggattgac ttctttttcc tcaatcaaat
    18361 aggcaatgcg gtgtgtcagc actctcgtct ttccacttcc tgcacctgcc attagcagta
    18421 atggtccgtc agtgtgaaga acagcttctt tctgacgggg gttcatcccg tttaataatt
    18481 cagctttgga tgacattaac aacatccttt cttttatcaa tcttgttaat tatagcattt
    18541 tcttggcttt cattaaacta gctagttcaa aatcatttgt ttctttttat ggatttttcc
    18601 ttgtcaattc gttgatttcg cgctaaatta aaagaaagcg aggcgtttga atggaaaaaa
    18661 aaggaaaaat caagaaatta gataagactc agatatattt tattctaaaa ggaatatttg
    18721 ttggactgct gacaggaatc gtcgttagcc tgtttcgatt atctatcgaa aagctaagcg
    18781 atttagttcg aagtatctat gaaatgagtc gggaacagcc gatttactta attggcctag
    18841 ctatttgctg catccttgca gcatttttcg tgggttatct ggtcaaaaat gaaccggata
    18901 tcaagggaag cgggattcca caagtagagg gacagcttcg cggggaatta tcaatgaact
    18961 ggttctctgt tttatggaaa aagttcattg gcggtgtact ttcagttggt gcgggccttt
    19021 ttctaggtag agaaggacct tctatacagc ttggcgcatc cattggacaa ggagctggac
    19081 aattgtttcg gagtccatct tcagaagaaa agattttaat ttctagcggt gcaagtagcg
    19141 gacttgcagc agcattcaat gctcctatcg caggattact ctttgtttta gaagaaatcc
    19201 atcactcctt ttctccactt gtttggctga catcatttgc ttcagcaatc actgctaact
    19261 tcgtctctct ttactttttc ggcttgagac ctgttttata tctaggtgac cttccttctt
    19321 tgccgttgaa atattatggt tccttagtga tcttaggagt acttctaggc atcttaggtt
    19381 ttatttatca gaaggtcttg ctggctttgc caaaatttta caaccgattg cctttaccag
    19441 cttattttca cgggttcgtt cctttcttgc tgattttgcc aattggttat ttatggccta
    19501 gtactctagg cggaggcaat caaatcgttt tagcttttgg acatgcttcc ttgccaatca
    19561 ttatgattat cggtttgttt gttcttcgat tcgttttttc catgatttct tacggtggtg
    19621 gtcttccagg ggggattttc cttccgatat tgacattggg ggctttaatc ggtagctttt
    19681 atggaaacat tatggttgat ttcgggatgg atccaatcta catcaaggat tttgtcgtta
    19741 ttgccatggc tggttatttc acagcgattg gaaaagcacc gctgacagca attattttag
    19801 tgacagaaat ggttggctct cttaatcatc tgatgccttt aggattggct gctcttgttg
    19861 cttatatcgt caatgacctt ctcggcggta atccaattta cgaatcatta ttggaacgtc
    19921 tacttggaaa tactcagacc gcctcactaa aaggtgcaag gacaactttt gattttccag
    19981 taacggcaga gagttcttta gacggattaa tggtacgaga cttcaattgg cctaaaaata
    20041 tgctgctaat ctctatacgc agaggaaatc aggaaatctt gactcacggt gatacgatca
    20101 tgaatgtagg agatattttg gttatcttga ctgatgaagc aaacttgcca agaatcaagc
    20161 aggaaatcga ggctaaatct tctatcacaa atttgaagca atcattttaa cgaacatgga
    20221 aaaactattt tacaacaact attcatctac actacttgat tctatatgat tctataaaaa
    20281 aagaagagac tgggaaaaac cagcctcttc ttttttattt ctatgcttct gctgctgcag
    20341 gagcttttct caataaacca taaatcacac cagatacgac tgctccgata gcaataaaca
    20401 ccaaatacat gaccgggtga ctcagtaaga atacaacgaa aatcccgcca tgaggagcca
    20461 gtaatttgat cccgaaagct ccgactaatc cgccagttaa agcagaacca ataataaaac
    20521 ttggaatggc acgaatcggg tcagctgctg caaatggaat cgatccttcc gtgacaaacg
    20581 atagtcccat cacgatgttc gtcaaaccag catcttgatc tgttttgctg aatttattct
    20641 taaacaaacg tgtagcaacg aaaattgcca atggaggtac cattcctcca gccataacag
    20701 aagccatcac gacacttccg ccagttgcaa cagtcgtagc taaagttgct gtaccaaaca
    20761 cataagcggc tttgttgatt ggaccgccta aatctgccgc catcattcca gctaataatg
    20821 caccaagtaa agcagcattc gaaccactca tacttgtcaa gaaaccgttc aatgcatcat
    20881 tgattgcttt catagggaca ttgaccagca acatcaagaa accagtaatc agtaagccaa
    20941 acactggata aaagaggatc gttttaattc catccaatga ttttggtaag ttcttgaata
    21001 atttacgtaa aaatacgatg acataaccag ctaagaatcc accaatcagt gcacctaaga
    21061 atcctgcacc gcctgtatta gctaatgcac cagcagcaaa tccaacgatc aaaccagggc
    21121 ggtcaccgat acttgaagca ataaatcctg ctaataccgg cagcatgaaa ccaaatgcag
    21181 cttgtccgat ttggttgaac cagctagcag cttgattgta attccctaga tttgataatt
    21241 gatcatgtgg cacgccaata aattggtcga tcatgaaaga aagcgcaata gcgattcctc
    21301 caccaataac gaaaggtaac atgtgtgata cgccgttcat caaatctttg taaattcttt
    21361 gtccgatagt accatccgca tttccttctt ctgaactgct ttcttttcca tcaccatgga
    21421 atactggtgc agtaccacta agggctagat tgatcagctc ttctggttta cggataccat
    21481 cactgacagg acgattgatc agttcttttc cgtcaaagcg attcatctct acttttttgt
    21541 ctgctgcaac gattacacct tctgcacggg cgatgtcttc agcagtcaat cggtttttga
    21601 ttccttctga accgttcgtt tcgactttga tctccacacc catttctttc gcttttttct
    21661 tcagagcatc ttctgccatg taagtgtgag cgatccctgt tggacaagct gtaacggcaa
    21721 caacgaattt tctttctgga tgagcagaag cttctgcagc ttgtgtttct tgttctgctt
    21781 tttcttctgc ttctttttgc tcttcagctg tttggaacaa gtcttggact tcttctggtg
    21841 tatttgcttc ttttaatttt cctacgaaat ctgcatcaat cagcaatcga gatagtgctg
    21901 ctaatgcttg caaatgtgtg tcgtttgcgc cttctggagc tgcgatcatg aaaaacagat
    21961 aagttggctg tccgtctaag gcttcataat ctactccttt ttggcttttt gcgaataaaa
    22021 ctgttgcttc tttaactgca ctgtttttcg cgtgaggcat ggcgattcca tcaccaagac
    22081 ctgtagaagt ttgtgcttcg cgggctagaa ttccttcttt atatgtttcg atatcggaaa
    22141 tccggccggc atcaaacatt ttttggacca tttcatcgat tgcgcctttt ttatcagtcg
    22201 cttgcaaatc catgatcatc gcttctttga ccattaaatc cttgatattc atgattatcc
    22261 tccttgaaac taatcaattt ttgtgatttc aacttctggc agcaactcat caatcaattc
    22321 gcgagctgcc aagtcatcag aaaaagctgt tgcgcttccg caagcaactc cccatttaaa
    22381 ggcttcgatt ggatctttcg tacggctgta agccccaata aagccggcaa tcattgaatc
    22441 ccctgcacct actgaatttt tcaatgggcg aactaataca ttcgttttat aagttccttg
    22501 ttccgtgaag agcaaagcac cgtctccagc cattgagatc agcacattct gtgccccttc
    22561 agctagcaat tttttaccat acggaatgat ttcttctaca gattggaagg ttgtattgta
    22621 aagctcagct aattcgtgat tgtttggttt gatcaacaat ggtttcttaa caagtgcatt
    22681 ttttaggtca tcacctgttg tatcaatcac gaaatctgca cctttttctc gaatgatatc
    22741 aatcagttct tgataaaatc cttttcgcag gctagccggc gcgcttcctg acaatacgac
    22801 gatatctcct tgtcctactg ttcctaatgc ttgcttcaac tcatcgattt cttcatctga
    22861 aatagatggg cccaaaccgt taatttcagt ttcagtatct gattttagtt tgatattgat
    22921 tcttgtatct tcttttacgg ttgtgaacaa agactggatt tgttcttttt caagccactt
    22981 cgaaataaat tcgccagtga accctcctaa aaaccctagt gctgtagatt gagaatcgat
    23041 ccgtttcaaa atacgggaaa cattgatccc ttttcctcca ggtaacttga agtcttcctt
    23101 cattcggttc aaatcgccta gttttaaccc atctacgcgt acgatataat caattgatgg
    23161 attaagtgtt actgtgtaaa tcattgtatt gcctccttaa ttgttgtctt ttcacggaat
    23221 tcattaacga attctaatgg acaacgctcg gttatgatcg tggctgcttc caaaggtgca
    23281 acagaggtga aggacaactg gttgaatttg gtatgatcaa tcaatacaaa tgtctcttcg
    23341 gaatttttca tggctaaacg tttaacagct gcctcttctg ggtccggagt cgtgaatcct
    23401 tgttcaagat gggcaccgtt cattcccata aatgctttat taaaatgaaa attttttagt
    23461 tgttctatcg cattagatcc taaaacagca ttagttgcca gctttatcgt tccacctaaa
    23521 ataatggtag caagctccaa atcaactaaa tttgccgcat gttgaacgga atttgtcact
    23581 actgtgatct gttttccttg cagaaaagga atcatttcta acgtagtaga gccagcgtct
    23641 aaataaatga catcttcgtc attaatggtt tcagctgccg atttggcgat ttgctgtttt
    23701 tcgtgaacgt ttttgagtga tttttcactc atattctgct caaaattcaa taattgctct
    23761 ttcttagctc cgccatgaac tctttctaga aacccactat tctccagctc ttgtaaatca
    23821 cgtcttatcg ttgattcaga agcattaaaa aggtttacaa gatcttgtga tttcacgatt
    23881 ttctgctgtt caagcaagtc taggattttt tgtctacgtt cctctgtaag cattttcacg
    23941 atcacctcaa tacaaataat agcatatttt ttttctactc gcaatataaa tcagtcaaaa
    24001 acgttcataa aaattataaa tcagtcaaaa aaaaataaaa aaccagtcaa aaagactggt
    24061 tttttatttt tttgtctatc atcaagccgt tttatcgttt tttattttcg cttcttgttt
    24121 ttttcatata aacagaagca ggttttcttt ccttatgaag aagataataa taaagtccca
    24181 tcccgccaaa catgaccaaa aaaccgatga ctgatcgaat aggaaaatta ggaacaaaaa
    24241 agagacagac ggataataat gatgccggaa ccattattcg ataatagagt tgtttatcca
    24301 ttttcttcac ctcatatttt ttgtaaagta atcaattgtt ctaattctcg gtaatctaaa
    24361 aattgcacac gcagatgctg ctcacctttc ttgacatcga attggatact tcgccgcttt
    24421 ttctttattc gtttgaaggt aacatcagag agtctatgtt tgatttcctt taacggtaat
    24481 tctgacaggg attctgaagg aacatagatt cttaaggata aaagatactc ttgatatttt
    24541 tcatctgtta cctctacttc tagatattcc ctaatataat aacaataagc ttcgattcca
    24601 ttatccaaag caacagtcgg atcgataact ttatagcaca tcatgcgcga gcgattctcg
    24661 tcaacccaag gaaaatacgc ctgagcattt agtgttccgc gaacacgcct ttctttataa
    24721 tctccttgtt gaatatcata actttttgtt acaaaaaatg aagcattctt tttaggaaca
    24781 ggttgcattt gtgcattctg atactggaca gattggatcg tatggtcagt gtaaatgtat
    24841 tcaacgaatg gcggtttcaa taaaccgaca ccattgtccc aagcaagatg atcaaaccca
    24901 agctggtccc taaaatagga ttgaaacatt tgattgtttg gcttttgcca gttaaagggg
    24961 tctcgataaa gctggataca tatgacttcg ttttctaatt ccttcacttt ttctacttgt
    25021 tggatgtcga gaaaaatcgg tttagggatg atttgatgat aatagcgact ataatacatt
    25081 tcccaacacg aagtgaaaca taaaccttgg taaaaaagat cgtatcctgg aaattggttg
    25141 caatcgacta ctacttcccc gttttgttgt ttcattttag gtaaatgttc gatttcatcc
    25201 tttttctcaa acaatagccg ttgagagatc tgttttgtat tgtgatagat aaactcgcta
    25261 taagctcgca tataggcgaa caccccatga gcaatcattt ttttctgaat gaagtctcga
    25321 atcaaaaatt gcttttcttt gaaaacagag atagacaata cattttttat caataattga
    25381 ttatcttcca agcgaaaact acattcgttc tcgtcatttc ctacaactaa ccaactttcg
    25441 tctcttaccg atgctagcat tattttttct tgaagctttc gaagatcata cggatccgct
    25501 tgttgtgtga aagagcggat aaatgttggc tggatcaaca gacgtgtcaa aatagcccaa
    25561 tcgcttattt cctgtttttt ttcttcttca tttaagtctg tgagctggat agtcatcaat
    25621 aatttatctt cgccaatcat tttctcacct ctttaaaatc tttttataat ttaattataa
    25681 ccaacgataa agaaaaacta aaactttatg tcttagaaac caatttggac gattacatga
    25741 tagactaagt aaaaagaaac aaagaggtgt ttacaatggg gttgaaattt gaacgtacag
    25801 ataatttaga taaacttttc gaatcatttg ctgtcgatcc ggaaaaaaca gttgcaccaa
    25861 aagaagagat cgatcgattt ctaaaaccac agccacaaaa tgaaaagaaa aaagacaaga
    25921 aacaaaagta agttaagctc aaaaaaagac ttaccagcca aacttttatg aaagagtttg
    25981 actggtaagt cttttttatt ctttttatga ttcaattgct tgttcgattg ctgcccacgc
    26041 ttcttctttt ccctgttttg taacggaaga aaagagaata aacgtgtcat tgcgatcaaa
    26101 atccaacgct tttttgattt cgctctcatg cttgttccat ttaccacgag ggatcttatc
    26161 tgccttagtg gcaacaacaa ttacagggat atcataatac ttcaaaaatt gatacatctg
    26221 gatatcttct tgagaaggct tatgacgcaa atctactaaa gaaacgaccg cacgtagctg
    26281 gtctctagtc gttaaatatg tctcgatcat ctttccccat tttgctcgct cagttttgga
    26341 tacttttgca tacccatatc ccggaacatc gacaaaatgt aatgcgttct cgatcaaata
    26401 aaaattcaat gtctgggtct ttcctggctt accggaagta cgggctaaat tctttcgatc
    26461 aatcaatgtg ttgataaaag aagatttccc tacatttgat cgtcctgcca gagctatttc
    26521 tggcaattgt gtatctggat attgttttgg tgccactgca cttatgataa tttcagcttg
    26581 atggactttc atggtctctc tccttaacaa ggtgatgaat aaagcaagcg gctgtcgcaa
    26641 aagccaaaat gacttttacg gcagcctctt ttcatcaacc cgcttttttc tctgaatctt
    26701 tatagattac tgttggctca ccagacaatt cagctgcttc ttttgtgata atcacttttt
    26761 cgatgttttc atttgaaggg atatcaaaca tcacatccat catgatttct tcaatgatgg
    26821 aacgaagtcc tcgagcaccg gtattgcgtt cgattgcttt tttcgcaatg gctctcaacg
    26881 cttcaggttc gaattcaagt tcggtattat ctagagaaag aagtttttga tattgtttga
    26941 ctagcgcatt tttcggctca gtcaagatcc gtaccagatc atctgttgtc aatttctcta
    27001 aagctgccat aacaggtaat cgtccgataa actcagggat caatccgaat tttagtaaat
    27061 cttcaggaat gatatgctgc atgacacttt cgtcttcaga aagtttttgg ttttgtgtac
    27121 caaatccaat cgtcttttcg cccatacgat tcttcacaat cgtttcgatg ccatcaaatg
    27181 caccacctac gatgaacaat atgttcgtcg tatcaatctg aataaattct tgatgtggat
    27241 gtttacgtcc tccctgaggt ggcacgcttg caacagttcc ttctagaatt ttcagcaagg
    27301 cttgttggac accttcgccg gaaacgtcac gagtaataga tacattttcg cttttacgcg
    27361 caattttatc gatctcatca atatagatga tccctttttc tgcacgttcc acattgtaat
    27421 cagcagattg aagcaattta agcaaaatgt tttctacatc ttcccccaca taaccagctt
    27481 ctgtcaaact tgtcgcatct gcgattgcaa aaggtacatt caatgttttt gctaatgttt
    27541 gtgctaaaaa tgttttacca gaccctgtag gtccgatcaa gcagatattg cttttttgca
    27601 gttcaacatc atctgtgttt tcttgtgctt gctgattgac acgtttatag tgattgtata
    27661 cggcaacagc taacgagcgt tttgcgcgat cttgtccaat cacgtagtta ttcaatactt
    27721 caagaatttc tagtggttta gggacttcag tgaattcgcg tacagcttct tcataaaatt
    27781 cttcatcaat gatttcttta cataggtcga tacattcatt acagatgtag acccctggac
    27841 cagctacgat ctttttcact tcttcttggg ttttcccgca aaaagaacag cgtacggtct
    27901 cattgctact tggattgtca tacatggtct tcacccctta acttgcaaaa agtatgcaag
    27961 aaactatcaa agactttaca gtcttttact atcataacat agtttagtaa aaagaaaaag
    28021 tgtttaacaa aggatcaact gacctatacc tttgcatttt ttactcttaa caaaaaataa
    28081 ggaactgtga caaaatcgtg tcacagtccc ttatttcgaa taaacaattt tctagaagta
    28141 gcttcttcag aaatgattta ttacttgatt tctattcttt cgcttctgtc gctgtacctg
    28201 tgatcaattc gattgctttt ttcattgtca catcatgttc cagcatatct tctgtcaaca
    28261 cgcgtttgat ttgttcgatt ggcatgttgt atgtttctga taattcttgg atttctgcat
    28321 tgatttcttc ttctgttgct tgaagatttt cagcagctgc aattgcttcg atcacaagat
    28381 ttgttcttgt gcgcatttct gcttcacctt cgaattgttt atgcaagtct tcttctgtag
    28441 aaccagttaa ttgatagtac atttcaggag agataccttg acgttgcatg ttgttcaaga
    28501 attcgtccat tgaacgatgt acttcatcat gaaccatcac gtgtggcaat tcaacgattt
    28561 ctgcgttttc aacagccata cggatcgctg cttcatcttt tgcatcgtcc gctgcttttt
    28621 cttttgtttc tactaattct ttgcgatatt tttctttcaa ttcgtctaat gttgatactt
    28681 cgtcatcaac atcttttgcg aattcgtcat ctaattcagg cacttctttt gcttttactt
    28741 catggattgt cactttgaag actgcttctt ttccagcaag atcttctgct tgataatctt
    28801 ctgggaaagt tacatttaca tctagacttt cacctgattt atgaccaact aattgatctt
    28861 caaaacctgg aatgaatgag cctgaaccta attcaagtga gtaattttcg ccttttccgc
    28921 catcaaacgc ttcgccatct acaaagcctt cgaagtcgat tactactgta tcgccatttt
    28981 cagctgcagc atcttctttg ataactaatt cagcttgtgc ttcttgttca cgtttgatac
    29041 gtgcatccac gtcagcatct gttacttcac ggtcttgttt ttcaacaact agatctttat
    29101 attgtcctaa ttttacttct ggtttcactg ttacttcagc agtgattacc cagtcttgac
    29161 ctttttccat gctttcaaca tcgatttttg gttgagaaac aggatcgatg ccagcttctt
    29221 tgacagcagc ttcataagct tctggtaata cagcattcaa tgcatcttca tataaagctt
    29281 cttcaccgta catacggttg aatacttgac gagaaacttt ccctttacgg aatccaggta
    29341 cgttcaagct ttttttcact ttattaaacg cagttgttaa tcctttttgg atttcgtctt
    29401 gcgcaatcgt aaattttaaa acaccatcat tcgtgccttt tttttcaaat gttgcagtca
    29461 tttaattccc tccgagttta gaaattcatt ttatacatca aaaacgtttc atgcatacct
    29521 ttaaatagta cactaaccct ttcaaattgt aaacatattg aatggataga atccttgttt
    29581 ttcataacat ttttatcttt ttcactgata aaatctaatg gaagaagatc atttgctgca
    29641 aaattttttc tctttttttt aaaaaatctt gtttttcatc ttctgtacct tcatacatcg
    29701 tttttcctgt acacgcctct acagtcagtt gaaaccagcg cttcgggtct tcgatgtatg
    29761 tatcatagac aggataaaca attgcagatt gcaacgtgaa ttcttcattt aacacggaac
    29821 taagcagcgc atcttcatta ccgtaacgat ctgaaaatac acataattgc tggtaacttg
    29881 cctgctgctg cggctttggc agatcttttg gtatgacttt gactaacttt tcttcaatcg
    29941 tcaaatagaa aacaggatgg cattcgttca attgcctaag tgtctccagt aattttgccc
    30001 ttgctaaaat atggacctct ggcgatacaa taagtttact cgctgtttgg ataaactcat
    30061 gataaggaag ctgttcgatc tcatcaatag acgcaagctg acgtgcaggt tccaacgaag
    30121 gaagttcctc cgatttattg tggagtgcct tgatcttctg cctctccatt tgtccggata
    30181 attcttctgc aaattggatt ttttcaatga cccgctgctc gaaagcttca taaaaatctg
    30241 tacttgctat caattttcta gcatataaaa aatcttgtgt actgattaac aagtcaaaat
    30301 aaaattcagc aaactcctca ttttgaagat actgtttttc atgcaagagt gcttgcttca
    30361 atgcttcttt tttctcgtcc aactcgtaaa gacaaaaaac aagcaggcta ttagcctcaa
    30421 agctttcttt caactcgtaa gctcgttgaa ataattcttt tgcctgctga tattgctggt
    30481 ttgctagtgc ttgttgtgct actcgcatta aattctcata tttttctgga aaatcaatcg
    30541 gcttcacgca gtgtcctcct cttgctcatg ttcagctcat gttcaatagt agcaaaacaa
    30601 aaaaagcatg acaattaaaa ataactgaag caccctctaa aagctagatt ttagatctga
    30661 cttttagaag gtgctttcaa ctgttactgc tttttattgc tcatcagctg ttgacttcct
    30721 ggttgaatgc tttgatcaga ttgatcgtaa caacacttgt attagtgaaa aaatcgccta
    30781 gatgttgctt tttctgatta aagattgctg gaagataccc taacatcagc aaaggaaatt
    30841 ttagaatgaa ccggcaagca ccttctctaa ctaaaacggt tgaccagcta agttcttctt
    30901 catcaaagct gatgactcga ataccaaaga tcattttccc aatcgtttga ccatgattga
    30961 atttcgtcaa taaaataaaa tacgctagat aaataagtaa ggctgtgaat ccgtagatac
    31021 tcagataaga atctgaaaaa ttaagccaac ctagattttt ggcaatacca agtgttaaac
    31081 gcgtaatagc tccgatacac aaaagatcga ccaagaaagc aaacattcgg ataaaaaaac
    31141 ctgcatagaa atacttagga aaatcatttg cttttggcgg ttcatttctt gtgtccgtat
    31201 actttttcca tttacgttgt ttctttttga tttcttcggg cgtcaaaggc tctctttcaa
    31261 aagagctcag taccttttgg gaaaagttgg agtcaggtcc tacagcaggc tgctgttgct
    31321 tatctttttc ttgttcctta ttttcatttg aaagctctga catgcttact cacctccgta
    31381 ataatacatt gcttttggtg attccgctgt ccctagattc tcaagaacgg ataagatctg
    31441 actagtctca gaaggcttca atccctggaa ttctgctagt ttacttccta accatgtact
    31501 agcaaaccca gatggcgtgg aagaatattc tactagttcc gcatcttcaa gctgttgttc
    31561 ctttcgcata gcttttaaag catcttcagg aaatcccaat tcatcgacta aacctacttc
    31621 ttttgcttgg acaccatcat aaatccttcc atcagcaatt tttttgactt cttcttcaga
    31681 tttattccgt ccctcgctga cgattgctac aaaacgacta tatgcgctgt cgatatatgc
    31741 ttgaagcacc gcttcttctt cttttgtctc tggtcgcgtt cctgaaccca tatctttcaa
    31801 cgctccgctt ttaactgttg tatcttccac acccagtttt tctagaagtc ctgaataatt
    31861 caatcctgac atgatcacac caattgagcc ggtcactgtt tcttctgttg caaagatttt
    31921 atctgcttga gcagaaatat agtagccacc gctagctgcc atgtttttca tgctgacata
    31981 cattgggata tcatgttctt tacgaatcgt atccaatttc ttagcgattt ccgcactttc
    32041 gtagacaccg ccaccaggag aatttacttc taaaaagata cctttgactg ttggatcttc
    32101 ttcaatcgcc ttcagttgtt ccataaaatt tttatgattg taaccttcag aagcaaataa
    32161 acctgaagac cctcccgcag caatcgttcc atctactgat aattttacga ttttttgcgt
    32221 actgtctccc tcttccaaca catttgaagc tagttcttca tccccatata accaactgtt
    32281 caatccctca atttgatcgt tttcctgagg cttagtcaaa ctagctgaaa ctaacgaaac
    32341 cacaaataat cctgcagcaa tcagaactgc tgcccaacgt ctcttattca ttctttctcc
    32401 tttctaatac aagtttttta tgacgatcac agtttctgac tgctcagaag tgtcgaaccg
    32461 ctctgctttt tcttcccgcg catacccgga aaacgactct tttgtcaatt catcttctac
    32521 cgtaaactca cgagcttcag caacttccaa ggcatcaggt gcgatttctt ggatgctatc
    32581 ccagccagta tctaaaaata ttttcgtatc atctgataca ccagctgatg cttcgaattt
    32641 ggacaattgt ttttttaaca ttcccagtgt catgatctca atttttgaat ccatttttac
    32701 tcctcctcta tccgattact ttctattgta ccgaactcct gaaaaagatg atagataaaa
    32761 acccattatt ttttgacata aaaagagcat ttgacaaagc atttgatata taccaattta
    32821 ttttttattc tttttttgtt gacaagaaca ttcgcaaaag gttatgattt taaatgtttc
    32881 cagataaaaa acaaaagagg aggatagtta taccttttag gatagcgcca ggtctgtgta
    32941 aacagatgac gaggagaggg tttatcgaaa gattcggcgg atggccctag ggactgcact
    33001 ctacagaaaa tccacaaaaa atatctgcaa agataaaaca aaaggatttt cctgcagctg
    33061 gaagcattca tacgaattca ttatagaaag taggatgttt tttatgtgcg gaattgttgg
    33121 aatgattgga ttaaaaaatg taacaccagg attgattgac ggtctagaaa aattagaata
    33181 ccgtggttat gattcagctg gtatctttgt ctcagatggc acaactgatt atctggtcaa
    33241 agcacaaggc agaattcaaa atttgaaaaa caaaataaat attgatacta caggaaatat
    33301 aggtattggt catacacgct gggcaactca cgggcaacct tctgaagaaa acgcccatcc
    33361 acatacttcc caaagtggac gcttcgtgct cgttcacaat ggcgtaatcg aaaactttga
    33421 agaacttaaa attgcttatt tagcaaatga ccactttatt ggtgagaccg atactgagat
    33481 tatcgctcat ctaattgaaa ctttcgcgaa ggatactaca acgcaagagg cgtttttaaa
    33541 agctttgcgg gtcatcaaag gttcttacgc tttcgctttg attgatcgga cagcacctga
    33601 tgttatttac gtcgctaaaa ataaaagtcc actattaatc ggactcggcg atggattcaa
    33661 tgtgattgcc agcgatgcaa tggctatgct tgctcatacg aaggaattcg ttgagattga
    33721 agatgaagag atggttaccg tcacatcaga aaaagttatt attcaaaatt ttgcaggtga
    33781 catcgtagaa cgttcttcat tcgaagcaca agtagattct tcagatatcg agaaaggtac
    33841 ctatcctttt tatatgctga aagaaatcga cgaacagcct attatcatgc gtcgtatcgc
    33901 tcaaaaatac gtaacagaag acaatcgtgt ctctcttgat caaaagctgg tcgatacatt
    33961 gtctgatagc gatcgaatct atatcgttgc ttgcggaact agttaccatg cggggttagc
    34021 aggtaaacag actctggaaa aattgacaca gatccctgtt gaggttcatt tagccagtga
    34081 atttggctat aacacacctc ttctttcaaa aaaaccattc tttattttct tgagtcaaag
    34141 tggcgaaaca gcagatagcc gccaagttct ggtaaaaatc aaccgtctcg gctacccttc
    34201 acttacgatt acaaatgttg ctggttctac tctttcgcgg gaagcatcct ttacactgct
    34261 attgcatgct ggacccgaaa tcgcagtagc atcaacgaaa gcttatactg cacaaatagc
    34321 tgtactaact ttattagcca aagctatcgg tgataaaaaa gaatttgaaa cttctcttgt
    34381 attcgatatc actcatgaat tgagtttagt tgctaatgcg atggaatcgg tcatcgccca
    34441 aaaagattat ttagaagagt tggcagaagt atatctcagc gatacaagaa atgcctttta
    34501 cattggacgt ggcgctgact acaacgtttc tcttgaagct gctttgaaat tgaaagaaat
    34561 cagttatatc caggcagaag gttttgccgc tggcgaattg aaacatggaa cgattgcttt
    34621 gatcgaagaa ggaacaccag tgattggaat catcagtgaa gaagtgactg gtgcgcatac
    34681 tcgaggaaac ctgaaagaag tcgaaagccg tggcgctaaa acactagtta ttgtctcaga
    34741 gggattagaa aaggaaagtg accagctggt gctaccaact gtccaccctt atctaactac
    34801 tctaaccaca gtcgtaccga ctcaattact tgcatattac gcaacgctat tacggggatt
    34861 cgacgttgac aaaccacgta atttagcgaa atcagtgaca gtagaataat aaaagaacaa
    34921 ggaccagtgt cagacatctg acactggtcc ttgttctttt att
//
LOCUS       DACDPS010000021        34579 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.021, whole genome shotgun sequence.
ACCESSION   DACDPS010000021 DACDPS010000000
VERSION     DACDPS010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34579
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.021"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            212..604
                     /locus_tag="IWJ07_001094"
     CDS             212..604
                     /locus_tag="IWJ07_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9332687.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="IWJ07_001095"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="IWJ07_001095"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAP9332688.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3165..4775
                     /locus_tag="IWJ07_001096"
     CDS             3165..4775
                     /locus_tag="IWJ07_001096"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAP9332689.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5067..5936
                     /locus_tag="IWJ07_001097"
     CDS             5067..5936
                     /locus_tag="IWJ07_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAP9332690.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="IWJ07_001098"
     CDS             6101..7372
                     /locus_tag="IWJ07_001098"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP9332691.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /locus_tag="IWJ07_001099"
     CDS             7369..8664
                     /locus_tag="IWJ07_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAP9332692.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="IWJ07_001100"
     CDS             8763..9026
                     /locus_tag="IWJ07_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAP9332693.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="IWJ07_001101"
     CDS             9471..9920
                     /locus_tag="IWJ07_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9332694.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="IWJ07_001102"
     CDS             9977..11395
                     /locus_tag="IWJ07_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9332695.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="IWJ07_001103"
     CDS             11395..12255
                     /locus_tag="IWJ07_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAP9332696.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="IWJ07_001104"
     CDS             12429..13202
                     /locus_tag="IWJ07_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9332697.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="IWJ07_001105"
     CDS             13349..13714
                     /locus_tag="IWJ07_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAP9332698.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="IWJ07_001106"
     CDS             complement(13862..15070)
                     /locus_tag="IWJ07_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9332699.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="IWJ07_001107"
     CDS             complement(15089..15367)
                     /locus_tag="IWJ07_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332700.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15560..16216
                     /locus_tag="IWJ07_001108"
     CDS             15560..16216
                     /locus_tag="IWJ07_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP9332701.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16658..17455)
                     /locus_tag="IWJ07_001109"
     CDS             complement(16658..17455)
                     /locus_tag="IWJ07_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAP9332702.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="IWJ07_001110"
     CDS             complement(17452..18636)
                     /locus_tag="IWJ07_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP9332703.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="IWJ07_001111"
     CDS             complement(18661..19908)
                     /locus_tag="IWJ07_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAP9332704.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="IWJ07_001112"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="IWJ07_001112"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAP9332705.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="IWJ07_001113"
     CDS             21888..22202
                     /locus_tag="IWJ07_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAP9332706.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="IWJ07_001114"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="IWJ07_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAP9332707.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /locus_tag="IWJ07_001115"
     CDS             22887..23531
                     /locus_tag="IWJ07_001115"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAP9332708.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="IWJ07_001116"
     CDS             23546..23875
                     /locus_tag="IWJ07_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332709.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="IWJ07_001117"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="IWJ07_001117"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAP9332710.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="IWJ07_001118"
     CDS             24863..25687
                     /locus_tag="IWJ07_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAP9332711.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="IWJ07_001119"
     CDS             25680..26027
                     /locus_tag="IWJ07_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAP9332712.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="IWJ07_001120"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="IWJ07_001120"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAP9332713.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="IWJ07_001121"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="IWJ07_001121"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAP9332714.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="IWJ07_001122"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="IWJ07_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAP9332715.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="IWJ07_001123"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="IWJ07_001123"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAP9332716.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31684..32451
                     /locus_tag="IWJ07_001124"
     CDS             31684..32451
                     /locus_tag="IWJ07_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9332717.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="IWJ07_001125"
     CDS             32441..34483
                     /locus_tag="IWJ07_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9332718.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT        11116 a         6130 c         7259 g        10074 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttt
//
LOCUS       DACDPS010000022        33607 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.022, whole genome shotgun sequence.
ACCESSION   DACDPS010000022 DACDPS010000000
VERSION     DACDPS010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.022"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            88..1749
                     /locus_tag="IWJ07_001126"
     CDS             88..1749
                     /locus_tag="IWJ07_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9332719.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(1819..2166)
                     /locus_tag="IWJ07_001127"
     CDS             complement(1819..2166)
                     /locus_tag="IWJ07_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAP9332720.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2250..3191
                     /locus_tag="IWJ07_001128"
     CDS             2250..3191
                     /locus_tag="IWJ07_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9332721.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3192..4235
                     /locus_tag="IWJ07_001129"
     CDS             3192..4235
                     /locus_tag="IWJ07_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9332722.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4241..5311
                     /locus_tag="IWJ07_001130"
     CDS             4241..5311
                     /locus_tag="IWJ07_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9332723.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5301..6254
                     /locus_tag="IWJ07_001131"
     CDS             5301..6254
                     /locus_tag="IWJ07_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9332724.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6266..7276
                     /locus_tag="IWJ07_001132"
     CDS             6266..7276
                     /locus_tag="IWJ07_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9332725.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7561..8382)
                     /locus_tag="IWJ07_001133"
     CDS             complement(7561..8382)
                     /locus_tag="IWJ07_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP9332726.1"
                     /translation="MNRFASKEEEKLYYEHEASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8479..8757)
                     /locus_tag="IWJ07_001134"
     CDS             complement(8479..8757)
                     /locus_tag="IWJ07_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332727.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9025..11034
                     /gene="metG"
                     /locus_tag="IWJ07_001135"
     CDS             9025..11034
                     /gene="metG"
                     /locus_tag="IWJ07_001135"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAP9332728.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11513..12100)
                     /locus_tag="IWJ07_001136"
     CDS             complement(11513..12100)
                     /locus_tag="IWJ07_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332729.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12275..13597
                     /locus_tag="IWJ07_001137"
     CDS             12275..13597
                     /locus_tag="IWJ07_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9332730.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13752..14528
                     /locus_tag="IWJ07_001138"
     CDS             13752..14528
                     /locus_tag="IWJ07_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP9332731.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14521..15090
                     /gene="rnmV"
                     /locus_tag="IWJ07_001139"
     CDS             14521..15090
                     /gene="rnmV"
                     /locus_tag="IWJ07_001139"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAP9332732.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15083..15967
                     /gene="rsmA"
                     /locus_tag="IWJ07_001140"
     CDS             15083..15967
                     /gene="rsmA"
                     /locus_tag="IWJ07_001140"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAP9332733.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16418..17047
                     /locus_tag="IWJ07_001141"
     CDS             16418..17047
                     /locus_tag="IWJ07_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332734.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17171..18280)
                     /gene="ugpC"
                     /locus_tag="IWJ07_001142"
     CDS             complement(17171..18280)
                     /gene="ugpC"
                     /locus_tag="IWJ07_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAP9332735.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18489..18911
                     /gene="mgsA"
                     /locus_tag="IWJ07_001143"
     CDS             18489..18911
                     /gene="mgsA"
                     /locus_tag="IWJ07_001143"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAP9332736.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19234..19770
                     /locus_tag="IWJ07_001144"
     CDS             19234..19770
                     /locus_tag="IWJ07_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP9332737.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(19810..21573)
                     /locus_tag="IWJ07_001145"
     CDS             complement(19810..21573)
                     /locus_tag="IWJ07_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9332738.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21576..23291)
                     /locus_tag="IWJ07_001146"
     CDS             complement(21576..23291)
                     /locus_tag="IWJ07_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9332739.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(24070..24294)
                     /locus_tag="IWJ07_001147"
     CDS             complement(24070..24294)
                     /locus_tag="IWJ07_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332740.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            24494..27262
                     /locus_tag="IWJ07_001148"
     CDS             24494..27262
                     /locus_tag="IWJ07_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9332741.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            27344..27763
                     /locus_tag="IWJ07_001149"
     CDS             27344..27763
                     /locus_tag="IWJ07_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAP9332742.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            27775..28245
                     /locus_tag="IWJ07_001150"
     CDS             27775..28245
                     /locus_tag="IWJ07_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9332743.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            28262..29041
                     /locus_tag="IWJ07_001151"
     CDS             28262..29041
                     /locus_tag="IWJ07_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9332744.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            29028..29849
                     /locus_tag="IWJ07_001152"
     CDS             29028..29849
                     /locus_tag="IWJ07_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9332745.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            29866..30915
                     /locus_tag="IWJ07_001153"
     CDS             29866..30915
                     /locus_tag="IWJ07_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9332746.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            30928..31932
                     /locus_tag="IWJ07_001154"
     CDS             30928..31932
                     /locus_tag="IWJ07_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9332747.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            32565..33584
                     /locus_tag="IWJ07_001155"
     CDS             32565..33584
                     /locus_tag="IWJ07_001155"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP9332748.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT        10964 a         5964 c         6878 g         9801 t
ORIGIN      
        1 tgatttttta ataattattc attaaactct taaacaactt cagaaaatat taattattct
       61 gaaaattcca ataaaggggt acgtaaaatg aaaaagaaat ttacatttgg tttggttgcg
      121 gtatgcggcc ttgtacttgc tggatgttat ggcggcagtt cagccacaag cagtagttca
      181 gcaaaaagcg gttcagcaga tggaggaggc gtatttaact tagtcgtgcc tcaagaaatg
      241 cctacagctg acttatcagt tgcaacagat acaatcagtt ttacagcatt gaataacgtt
      301 tatgaaggaa tttaccgtct tgatgaagac agcaaaccac agccggcagg agctagcgag
      361 ttggcagaag tcagtgaaga cggattaact tacaaaatca aattacgaga agacgcaaaa
      421 tggtcaaatg gtgatcctgt tacagcagcg gattacgttt atggctggca acgaacagta
      481 gacccagcta cagcttctga atatgcttac ctgtatgcgc cagttgaaaa tgcagaagac
      541 attacagctg gaaagaaaga taaatcagaa ctagggatca aagcagttgg tgactatgaa
      601 ttagaaatca aattgacgaa acaaacacca tatttccaat atctgcttgc tttcccatca
      661 tttttcccac aaaaacaatc cgtagtggaa gaaaatgggg atgcctatgc atctgcaagt
      721 gataaagctg tttataatgg accattcaca ttagaagatt ttgatggacc aggaacagat
      781 acagaatgga cttataagaa aaatgatcat tactgggaca aagatacagt aaaactttct
      841 gaaatcaaag tcagtgtagt aaaagaatct tctactgcat tgaacttatt caaagatggc
      901 caagcagatg acgtgatctt gtctggtgag ttagcacagc aaaacgcaaa cgatccagct
      961 tatacttctg ttaaagaagc gcggacaagc tatatcgaat tcaatcaacg tgaaaagaat
     1021 tcaccattca acaatgtgaa cctaagaaaa gctatttctt attctatcga tcgtgaagct
     1081 ttagtaaaac aagtactagg tgacggatca gttgtctcta caggattgat cccatcagat
     1141 atggtaaaaa gtccaacaac gaacgaagac tttgcaaaag aagctggaaa attagtttct
     1201 tatgacaaag ataaagcaaa agaatattgg gaaaaagcga aaaaagaatt gggcgttgat
     1261 tctttagaat ttgatttgat ggcttctgat gatgattcat cgaaaaaagt aatcgaatat
     1321 gttcaaaact caatccaaga aaacttggat ggcgtaacag tcaaaccaac acctgttcca
     1381 ttctctgtac gtcttgaccg ctcaacttcg ggtgattttg acgcagtact aggcggatgg
     1441 gctgctgact atgcagaccc aagcagcttt actgatttgt ttgtcacagg taattcatac
     1501 aaccgtggtc gctggagcaa tgcagaatat gacgaagcag ttaaagcagc ttcatcaaca
     1561 gacgctggcg atgaacaagc ccgttgggat gatttccaaa aagcaatcaa gatcatctct
     1621 gatgatatgg gtgtcgttcc ggtgtaccaa aaagcagaag gtcacctagt gaatgaaaaa
     1681 atcaaaggta tcgttcacca tgcggcaggc gcttcatggg attacaaatg gacatacgtt
     1741 gaagaataat cgttaatacc gtgagcatga gaaaaaacga ggctgcggca aatgtcgcaa
     1801 cctcgttctc atattcactt atcctaacaa agaaaaaagc atgaaaatag cagaaagagc
     1861 aattagtatt ccggcaatga tcagccaaaa gctgtacata ccgtagctgg cagacgagag
     1921 cgaggaaaat tctggttttt tctttcggtt ccttgttcgt gcaagatgga ccgatcgctg
     1981 aaaatgatcg aagaaacccg aagaaaagac ccataaaaaa ccgccgataa tcagaaacgg
     2041 caatgaccat aagaatagat cattagataa ttgaatgagt gtaaaggaat ggttgatgag
     2101 gttttttatt aaaataacca gaatcagtag aaaagctact gcgtagggga tagatttttt
     2161 tttcatttga ttgctccttt atttttggtt cattatctaa taatgtagcg aaacatctgt
     2221 cagaagtcaa aagctagggg gaaacattag tgaatagtta tatcaaatat gtattaaaac
     2281 gtgtcttttt catgatcatc acgctttggt taattgcaac aatcaccttt ttcttgatgc
     2341 aattgctgcc gggcacgcca tatacgaatc aggaacgttt gagtcctgaa acaatcgaga
     2401 tgctaaataa acaagtcggt ttggataaac cagttatcgt acaatatggg atttatcttt
     2461 ctaatttgct tcaaggagat tttggaattt ccttccaatt taaaaaccag ccagtcgcac
     2521 atttgttagc tggaagaatc ggaccatcct tgcagttagg gttacaggca attatctttg
     2581 gaacagtcct agggacgatt ttaggaacga tctctgccat gaagcaaaat acatgggcag
     2641 atacatcttc tacactcgta gcgatattag gtcgttccat tccaaacttt gtatttgctg
     2701 ttttattaca atatatcttt gcaatcaaat tacaagtttt gccaatcgca aaatgggatg
     2761 gctttgtgta cacaatctta ccaacaattg cgttggccat gtcgccgctt gctgactctg
     2821 ctcggtttat ccgtacggaa atggtggaag tactgcatag tgattatgtg gaattagcaa
     2881 aagcaaaagg attgagccgt tgggaaatcg cctttaaaca tggtcttcga aatagtttga
     2941 ttccattgat gactttatta ggaccgttag ctgtagcatt gatgaccgga tcactggtgg
     3001 tagaaaatat ctttgctatt cctggtatcg gggaacaatt cgttaagtca atcacaacaa
     3061 atgattatcc tacgatcatg gctgtaacga tcctttactc atttatgctg atttttgtta
     3121 ttttgatcgt ggatctattg tacggtctag ttgatccaag aattcgtgtt tcagaaggga
     3181 gccgaggata aatggaaatg ataccaaagc gttatgaaac agttgcagat atccctgcgg
     3241 atgaatttct tccattacaa aaaaataccg aagaagaacg tgaacagatt actgcacctt
     3301 ctttgaattt tatccaagat tcatggcgcc gtttaaagaa aaacaaagct gccgttattt
     3361 caatggtttt actattggtc atcattttga tctctgtgat cacaatcttt gtttctcctc
     3421 atgatccaac tgcccaaaac gtggcttata tcaatttgcc tcctcgtatt ccgggaatca
     3481 atatcgatgg attgaatgga aaagcaatgg ttggtggtga acttgttgat aaatatgctc
     3541 aagcaaacgt cccttcaaac gtaaatttct ttttaggaac agacggtttg ggacgtgatg
     3601 tattaagccg cttatttatg ggaatgagaa tctcgttgct gatcgcattt attgctgcat
     3661 tactagatgt aacgattggt gtagcttacg ggttggtttc tggtttgtta ggcggacgtg
     3721 tcgataacgt gatgcaacgt tttttggaaa tcttatcagg aattccaaac ctcgttgtca
     3781 tgattttgat gttggtcgtt tttgaaccag gaattttttc aatcgtggct gctatggcca
     3841 tcacaaactg gataccaatg gcacgtatcg ttcgtgcaca aacaatgaaa ttaaaagatc
     3901 aagaatatgt tttagctgga ttgacattag gtgaatcaag aatgaaaatt gcattcaaac
     3961 atattttgcc gaatatctct agtgtcatta ttattcaaat gatgtttagt attccatctg
     4021 ctattttctt cgaagccttt ttaagcttta tcggtttagg tcttacgcct ccgtcagctt
     4081 cattaggaac gatgttgagc gaagggtaca agacattcct gtatctgcct tatctattat
     4141 ggattccggc agtaacttta tcggttatca tgattggctt taacttactt gctgatggat
     4201 tgcgtgatgc ctttgatcct aaaatgaaag agtgaagacg atgacgaaaa ttttagaagt
     4261 gaaagactta gaaatctctt ttgatacgta tgctggaaag gtacgagcga ttcgcggtgt
     4321 caattttcat ttgaataaag gagaaacatt ggcgatcgtt ggggaatctg gaagtggaaa
     4381 atcagtgacg accagaagca tcatgcgctt gctgtcaagc aacgccaata tagatgctgg
     4441 tcaaatcctc tttaaaggta acgatatcgt tggaaaatca gaaagagaga tgcaaaaaat
     4501 ccgtggtaaa gaaatagcga tgattttcca agatccaatg acctctttag atccgacgat
     4561 gcccattggg aagcaagtcg ctgagtccct tagaaagcat aataaagttt caaaaaaaga
     4621 gggacaaaaa gctgcattag aattgcttaa attagttgga atacccgaag ctgagaaacg
     4681 tatcaacagt tatccgcatc agttctcagg aggtcagcgt caacggatcg tgatcgcgat
     4741 tgcactggta tgttatccgg aaatcctgat tgctgatgaa cctacaacag cgcttgatgt
     4801 gactattcaa gcacagattt tggagctgct gaaagaaatt cagacaaaaa tcgatacatc
     4861 aattatcttt atcactcatg accttggtgt agtagcgaat gtcgctgatc gtgtcgctgt
     4921 catgtatgga ggaagaatcg ttgaagttgg aacatcggaa gaaattttct acaacccgca
     4981 acatccctat acttggggac tgcttggttc aatgccgaca ttagatagtg cgaacgatcg
     5041 attgtatgct attcctggtt ctccaccgga tttattgaac ccgccaaaag gagacgcatt
     5101 ctatccaaga aatgaattcg cgatgaaaat cgatgcagaa caagaaccac cattttttga
     5161 actatcaaaa acacacaagg cagcaacttg gctacttgct ccgcaggcac ccaaagtgac
     5221 accgccagaa gaaatccaac gtcgctgggc tatctttaaa gagaaacaaa aacatactta
     5281 cggtcaagga ggaattgctg atgccaaata aactacttga agttaaagga ttgaaacagt
     5341 atttcaatgt aggaaaaaag aatgaagtac atgctgtaaa tgacatcagc tttcacattt
     5401 atgaaggaga aacattcggt ttagttggag aatctggaag cggaaaatcc actactggcc
     5461 gtacgatcat tcgcttgaat gaacctactg atggtgagat attattcgac ggtcaagata
     5521 taacgaagat caaagataaa aaaggtttga cgaaattccg gcatgatgtt cagatgatct
     5581 ttcaagatcc atacgcttcg ttgaatcctc gtatgaaagt cagagatatc attgccgaag
     5641 gaattgatgt taatggacta gctaaatcat ctgaagagcg tgcaaaaaaa gtcgatgatc
     5701 tattgagaac tgttggattg aatccaagcc acggtactcg ttatcctcat gaattttcag
     5761 gtgggcaacg ccagcgtatt ggtattgcac gagcattggc agtaaaaccg cgttttatca
     5821 tttgtgatga accaatttct gcactagatg tgtcgatcca agcccaagtg gtcaatttgt
     5881 tgcaagatct ccaaaaagaa catcaattga cctatctatt tattgcacat gatttgtcga
     5941 tggttaaaca tattagtgac cgaatcggtg taatgcataa cggcttgtta ttagagatgg
     6001 gaacaagtga tgaaatctat aatcacggtg ttcatcctta tacagaaagt ttgttatctg
     6061 ctattcctct accagatcca gaccatgaga gaaaacgcag aagaatcaaa tatcaacctg
     6121 aaccagatga cggacaagta cgtcaacttc gtgaaatcgc accagaacat tttgtctatg
     6181 caactgagca ggaagtccca tattacgcaa aaaaactaaa acgacaaaag gaatctcttc
     6241 tggttgctaa ctaggaggga catagatgct aaaagctttt aaattccgta tgtatccgac
     6301 agaagaacaa aaacagcaat tgattcgtac ttttggatgt gctcgattta cctataacca
     6361 tttgctgaaa aagcggcaga aaagttggca acaaacagga gtagccaatt tttcgctcac
     6421 acccgctaca ttaaaaaaag aatacccatt cttaaaagaa gtagacagtc ttgctttagc
     6481 aaatgcacag cttaatttgg atcgggcttt tcgcaattat ttcaagggac gtgccagctt
     6541 cccaaagctt aaaacgaaaa aaagtatgtg gcaatcttat acgaccaaca accaaacccg
     6601 tacgatctat ctggaaaatg gctacttaaa gttaccaaaa caaaaagaac taatcaagat
     6661 aaatcagcat cggccagttg aaggatcgat tcgctccgct acgatttctg ctcgatataa
     6721 tgaagaattt tatgtcgcac tactttgtga tgtttcgcca gtaaaaaaag aatctttagc
     6781 taaatggatc gggatcgcct atcatccgaa gacattgatc caaacatctc gacctctaga
     6841 agtaacttta ccaaaatttc accagacaga agaaaagctg caacatgcac agcggaaatt
     6901 gaatgtgaaa gtccgctccg ctcaccatag aaagatcagg ttagatcagg caagcaatta
     6961 tcagaaacaa aagagaaaag taatggatct ttatctgaaa caaaagaacc agcgagaaga
     7021 ttatcttgaa caattatctg ggaaattggt caaacaatat gactacttat ttgtagaatc
     7081 ttttccaaaa gaagaggcac atgctgattt ttctattcac gactggcata aattaataac
     7141 aaaattgcgt tataagtccc aatggtataa taaaaaattt cttttgatca ataccgacgg
     7201 agcagaagaa agcaattccg taagaaagag ccaagtagta gaaaaaatgg gcagacattc
     7261 ggttatcaag ggataaaaaa tgaatgaaac tgataggagc gttagggtta gccttgttaa
     7321 taaaaagtct taatgacttt tgtttctagg aatttttctt ttgaagaaaa gctcactaat
     7381 tatgacaaac caataaaaca atagcagaaa aagttcgtac gagctttttc tgctattgtt
     7441 aattttttat tcagaaagtt tacgaataca tcaaaattct gaaacaaaaa acgtaactca
     7501 caaagagttg tttttgcgtt ttttcttgaa tgattaggtc tgaatctttt gctcacaacc
     7561 ttattcatgg tgccccggct tgagctgata gatttttgaa ggacggccga ttcctacagg
     7621 acgttcaccg atctcatcga aatattggag catagctttc ttaaaattgg aatgatcgat
     7681 cgttttataa tcgatcccta agaatttagc aaatactttt cgagcttccg taatcgtgaa
     7741 atctttacct aaaacttgta gaacttgcgg ctcgtgttcc attttattga ctacacggtt
     7801 gaatgctttc aagatgattt gactatggtc aaaagccaaa gtatctttgc cagaagactc
     7861 tccagtgact aaatcaagcg agatagccac ttctccgcta gataaatgga tcttgttact
     7921 atgacgttct agtgtgaacc agcgtacttc cttggcatca tctccggcga ttagcggttc
     7981 ttctccaata aaggctaagt agctgactgt gacgacccag cctcgcggat ctcgattagg
     8041 cgtactgaat gtatgcagtt gctcaatatt ttcttttgaa ataaccacac cagtttcttc
     8101 ctttgtttct cttaagacgc tttctccagt tgactcatcc ttttggacaa agccaccagg
     8161 caatgcccat gaatttcgat aaggatgccc ttttctttgg atcaacaaaa ctttcagctg
     8221 atcttcttct ttgttatagc acatcagtac gatatcgaca gtcaaagagg gcttgtcata
     8281 ttctggacgt tcctgttttt gataccagtt aagaaattct tcttcacttg cttcatgttc
     8341 ataatataat ttttcttctt ctttagaagc aaaacggttc atctgaccac ctccattgac
     8401 agtaaaagta ctttttacct tattgtagca gactctgaaa aaaaaccaag gatttacttg
     8461 aaaaatctag gttatcattt aaatatgaaa aataaatgga agtatgagat tgaataaaaa
     8521 gaaaagcaga taaaacggca atactttata ccgctcttca cgaatggcaa agaagccaca
     8581 aataacgata tatacgtcaa tgataaaacg caaaatccaa gctgctgtca taaaactgtt
     8641 caaatgccca tggaggttaa aataaccata catacgaata taaaaatagc tgaatattaa
     8701 atgtgctaat gaaagaaaag caatactaag tgtaaaaaaa agatgtttct tctgcatgat
     8761 tgttccagtc ccttactaag atagatagtc tttattatac cacgtgtgag gggagaaata
     8821 aaaagccgtt ttttagtaga tatgaaacaa aacaaaaacc cgcatttatt caacctattc
     8881 atccttttaa aggatagatt gaacgtagat agaagtcgtt tatccagtct ttttcatttt
     8941 gaaaaaaagc taacagcatg ctttttttat ggtatactag caaggaaacg aattcgcatc
     9001 atgcgataaa ggaggaagag ccccgtggca gaaaaggaaa ctttttatat cactacgcca
     9061 atctattatc caagcggcaa acttcatatc ggcaactcgt atactacgat tgcatgtgac
     9121 gcaattgctc gttataaacg gttgatgggc tttgatgtgt tttatctcac tggagtggat
     9181 gaacatggtc aaaaaatcga aacaaaagca gaagaactag gtgtaaaacc gcaagaatat
     9241 gtagataaaa tggcggctga tgtcaagaaa ttatggaaaa cgcttgatat cagttatgat
     9301 aaattcattc gcacgacaga tgattaccat aaagcagctg ttcaaaaaat atttgatcgt
     9361 ttattagaac agggagatat ttacttaggt gaatatgaag gctggtactc tgtttctgac
     9421 gaagaattct ttacagaaac acaacttgca gaagtgtaca aagatgacga aggtaacgtg
     9481 atcggtggta aagcgccaag tggacacgaa gtagaattgg taaaagaaga atcatacttc
     9541 ttccgcatga gcaaatacgc agaccgtcta ttggaatact atgaagaaca tcccgaattc
     9601 atccagccag aatctcgtaa aaacgaaatg atcaacaact ttatcaaacc aggattagag
     9661 gatcttgcag tttcacgaac aacttttact tggggagtac cagtcaagag tgatccgaaa
     9721 catgtggtgt atgtatggat cgatgcatta tccaattata ttactgcact aggatatggc
     9781 tccgaagatg aaagtctatt cgaaaaatac tggcctgcag atgttcagat ggtcggtaaa
     9841 gaaatcgttc gctttcatac gatctattgg ccaatcatgc tgatggccct tgatttgcct
     9901 ctacctaaaa aagtatttgg gcatggctgg ctattgatga aagacggaaa aatgtctaaa
     9961 tcaaaaggaa atgtcgttta tcctgaaatg cttgtggaac gttatggttt agatgcactc
    10021 cgttattatt tgcttcgtgc tgttccgttt gggtcagatg gtgtctttac gccagaagat
    10081 tttgtttccc gtgtcaatta tgatctggca aatgatctag ggaacttatt gaatcgaacg
    10141 attgctatga tcaacaaata ttgtgatgga ctcgttcctg actatgcctc acttgtcact
    10201 ccatttgata gtgaactgtc tacgacggct gctaacgttg tcggacgtta ccatgatgca
    10261 atggaaaaaa tggaattcaa tacagcattg gctgaaatct gggtgctgat ttctcgtgca
    10321 aacaaataca ttgatgaaac agaaccatgg gtattagcaa aagacgaaga aaagaaagct
    10381 gaattgaata gtgtcatgat ccatttagct gaatctttgc gtatcgcagc gatattactt
    10441 cagccaatca tgacagaaac gccagagaag atcttcaatc aactaggatt ggactctgaa
    10501 acaatgaatc ttgaaggatt acactttggc gaatttcctt caggaacaaa agtagttgca
    10561 aaaggtacac caatcttccc acgtttagat atggaagaag aagttgcttt tattcaagaa
    10621 aaaatgtctg aaggtactca aacaaacgaa gatacagtca aatgggatcc agaagaaaca
    10681 gaacttgttt caacgaaaga aaaacaaatc aaatttgatg tatttgaaaa agtagaacta
    10741 aaagtagcag aagtcatcaa ctgtcaaaaa gtagaagggg cagataagtt gctgcaattc
    10801 cgactagatg ctggagacag ccaagaccgt caaatcttgt caggaatcgc tgaattttat
    10861 cctgatccaa gcgaattgat cgggaaaaaa gtcgtcattg tagcaaatct gaaaccacga
    10921 aagatgcgtg gacaaatcag tcaaggaatg atcctttctg cagaagcacc agatggctct
    10981 ttacaagtaa tcgaagcacc aaaatccatg ccaaatggct cagaaatcgc atagaaattc
    11041 aaagggggta tgacactagt tgtgtcatac cccctttttc cgaaatttga gaagcaattt
    11101 tcgtaaattc cttcttatta cttgaagcca atcatataat acagaaacag agaactatcc
    11161 aaaataaatc ctagaaaaag aagtacgagc ttcgctgatt tttgttaatt tagaataata
    11221 ctaataggaa tcgtaaaaac agaaaaaaag cataatgata aagggtcggg cagattttaa
    11281 ttgattcatc tggtactctt ttttattgtc cttacattca taaagtttga atgcaatgac
    11341 tttattacat ctatatttat atctaacagt agcgttgatg tgttaattat aagttctatc
    11401 actttatgtt caattagttt gtgctcttat atgtgtgatt gaaaatatag ataaaaaaac
    11461 aatcatcggc tttgacttcg atgattgttt tctttatttt gtaaaaatcc tatcacttga
    11521 ttttatggta accttttatt tttgtcttca aacggcttgg taagagagca gtaagcagtg
    11581 caaatgggat cgaaaagaca taccagaaaa tcgtacgtcc gataccataa tgttgataga
    11641 gctttgtcaa catcacgtgt aacaagatca accctaaaag caaggagcca gctataaacc
    11701 aatgattatc ccaatgcatg acttcactaa gtgccatcac tccaaaagga tagaaataaa
    11761 aatagatccc gactaaggtc atccgccagt tctttgtaaa tgttccgata ttcaataaaa
    11821 agaaaatcgc tgaaagcaaa ataccgatag gcggaaaaag cccaaccaaa aaggaataca
    11881 acatccccca caagatcatc acaggacctt tgatcaatgc aacgattgcg atgaacaaag
    11941 tcagcaatat tagtttttgt gataacgttg caacagtcat taatccaaat aaaattaatg
    12001 tgaaccaatg ccaatgggac tgctgttgtt ctttacggga aaaatcgacc cggttggata
    12061 aataatctgc taatttcgga taagaacgct taaaatccat aaactgttcc tccttcgtgg
    12121 aaaaggtagg tttagtatac gaaaaataaa aaagaaatac atccgtctta agagggagtt
    12181 tttcactgaa agtggagaaa aaagaatggt aaattaaaat attctcagtt atactgaaaa
    12241 ttataccaaa tttcaaagaa gaaagaggat gggaatgaaa ggcaaactaa aacgagaaat
    12301 caatctgttt ggcgcattag ctaccgttat gggaaccgtg atcggtgcag gcgtcttctt
    12361 caaaactgct gctgttactg ctagtactca gtctacaagt ttgacattgc tagcttggct
    12421 tttgggtggt ttcttaacga tctgtgctgg attgacagtg gctgaattag ctacagcaat
    12481 cccggaaact ggtggagctg taaaatatat tgaagctgcg tatggaaaat tgcccagctt
    12541 tttattagga tgggcgcaaa gcttgattta ctttcctgct aatattgctg cattatcgat
    12601 tatctttgcg acacaattga cgaacttatt acaattatct accgattatc ttttgttgat
    12661 tgcagtgatc accgctgttt ctgtgacagg gttgaatttg ttagggacaa aagtcggtgc
    12721 ttctgtacaa tcagtgacgt tgattgttaa attaatacca attgctgtga ttgtcatatg
    12781 gggattattg accccaggac aaggaactgt ccagttattt ccaatcgaag ctggaaaaga
    12841 tgttactttt gtcgaaggat tgagcagtgc gttgttagca acgttgtttg cttatgacgg
    12901 ctggttaggt gtcggtgcaa tggcagggga aatgaaacgg ccggaaaaag atttgcctaa
    12961 agcaattatt cttggtttga gttttgtaac agtcgtttat ctattgatca attttgtttt
    13021 tttgaaaact ttgccgattg atcatcttgc cggtaatttg aatgcagctt ctgaagcatc
    13081 agacgtcatt tttggaggaa tcggtggaaa gctggtcact attgggatct tgatctcggt
    13141 atacggtgca ttgaacggtt atacactaac tgggatccgc gtgccttatg cgatggcttt
    13201 agaagacgag ctacctttca gtaaacagct gacaaagctt tcaaagaagt ttacggttcc
    13261 ttatgttcct gcggttttcc agttagcagt agcttgtatt atgatgagcc ttggttcttt
    13321 cgacttttta acagacatgt tgatttttgt gatgtggcta ttcagtttac tgatttttat
    13381 cggtgtcttt attttaagaa aaaaagcgcc ggaattgcca cgtccatata aagtaccact
    13441 ttatccaatc gttccgatta ttgcgattct cggtgctatt ttcattttag gtatgacgat
    13501 gatgacgcaa acgaaattag cattgatcgg tataggtgtt accttgatag gtatcccagt
    13561 ctattatcaa aaaaagaaaa aacgctcaga agagtaaaat atttcaacaa cagacagaaa
    13621 aagtacgaac taggtcctct agatcttcgt gctttttcta tttcaataaa acaagttctt
    13681 ctattttgat ttttgctact tctgcaagta gtgcttatgt gctaaagtaa gaatgaaaag
    13741 gagagataaa catgatattt gattctcata cccatttaaa tgctgaacag ttcaatgaag
    13801 atattccaga aacgattcag cgagcacaag aaatgggggt aacgaaaatg gcggtcgtgg
    13861 gcttcgatac gccaacgatt gaaaagtctt tgcaattaag ccatgactat ccaaatatat
    13921 acagtattat cggctggcat ccgacagagg ctggcagtta tacgaaagaa atcgaaaata
    13981 aattacaaga acaattgact ttaccaaagg ttgtagcatt aggggaaatt ggcttggatt
    14041 atcattggat ggaagacccg aaagaagtcc aagataaagt atttcgcaga cagattgcta
    14101 tcgcaaaaga aatgaaccta ccaatcagta ttcatactag agaagcactt gaagacacgt
    14161 atcaaatcct aaaagaagaa gatgttcgag atatcggtgg aatcatgcat agtttcagtg
    14221 gtgattatga atgggcaaaa cgatttcttg acttagggat gcatatttca tttagcggtg
    14281 tcgtcacttt caaaaaagca ttagacgtcc aagaggctgc agcaaatgta ccaatcgaac
    14341 gattacttgt ggaaacagat gccccttatc tggcaccagt tccttttaga ggaaagcgaa
    14401 atgaaccagg ctatactcgt tatacagtag aaaagattgc tgaacttcgg aatcttccga
    14461 cagaagaagt ggctgcccaa acatggacaa atgcgcaccg attatttggt ctaagcgaac
    14521 atggataaga tgaagatcca agagattata gtcgttgaag gaaaagacga tacaagaagg
    14581 ctgcgggaag tagttgatgc agatacgata gaaacaatag ggtctgcaat caatgaagag
    14641 atcctgatgc aaattgaaca tgcccaagag actcgtggag tgatcgtttt taccgatcct
    14701 gatttttcag gagaaaaaat aagaaaaatc attatggaag ctgttcctca ggcaaaacat
    14761 gcttttttgc cgcgcacaca agctaaacca aagaaaaagg gaagccttgg tgtcgagcat
    14821 gcaagtgatg ctgctatttt agaagcattg aaaaaggtcg tgacgccaga catttcccaa
    14881 acggctgttt ctgaaatcac tagacaagac cttttagact atggtttaat agctggggca
    14941 caagcaaaag aaaaacgaga aaaactaggt gacgagttgc gcatcggtta cacgaacggc
    15001 aaacaattgg aaaaacgttt gaagatgttt cgtattacgc cgaaagaact cgcccaagca
    15061 atgaaaatag tggaggaaca acatgagtga atatcgtgaa attgcgacac cctctagaac
    15121 aaaagagatt ttattgaaac atggtttttc atttaaaaaa agtttaggac aaaattttct
    15181 gactgaacca aatattttaa ggaaaatcgt agaaactgcg caaatagatg aacatacgaa
    15241 cgtgatcgaa gttggaccag gaatcggtgc attgacggaa caattagcca agcatgcaaa
    15301 acaagtcgta gcatttgaga tcgatgatcg tttgatccct gtattggctg atacgatgca
    15361 accctatgac aatgtgacta ttgtacatca agatatttta aagaccgatt tgtcaacagc
    15421 tgttagagaa acatttcatg aggagcttcc gttaaaagtc gtagcgaatt taccttacta
    15481 tattacgaca ccgatcatga tgcattttct tgaatcagat ttggttgtgg atgaactagt
    15541 cgtcatgatg caaaaagaag tagctgatcg tatctctgct gaaccaggaa cgaaagctta
    15601 tggttcatta tcgattgctg ttcaatatta tatggaagcc agtttagcat tcatcgtacc
    15661 aaaaacagta tttgtccctc agccaaatgt agattcagcg attttgaaac tgacaagaag
    15721 agatataccg gcagtcgaag tgacagacga aaaagcattt ttccgattga caaaagcggc
    15781 atttcagcaa agaagaaaaa cgctttggaa taacttgcag cattcgtatg gcaaagatga
    15841 ccagacaaaa gcatggttag caaaaagctt ggaaacagct ggaatcgatc caaaacgtcg
    15901 tggggagact ttgtcattac aagaattcgc cgcattaagc aatgcgatgt cagaaaatat
    15961 gcagtaaaaa aagaggttgt gacagaagtt gtgaaaaagc tgtcctgcat caagtaataa
    16021 gaacagccaa acaaaaatag ttcctcatgt ttttcgtttg tttgggctta ttaccgcaga
    16081 tgcaagcttt tgaacaccgt ttaagaaaga ggttgtgggc ctgccgttta actctaagtc
    16141 acaaggaaca atttcaaaaa acagcttctc atgtttttga gttagttggt cttattactt
    16201 gatgcaagct tttgaccact attatgccga acaaggagat gtgacacaaa ttttgtcaca
    16261 tctcctattt ttgtttcttg atcaaaaaat tcttatactt gatactcaca ctcattttgt
    16321 atggatttag aaaagaatat gtgtcttgac agttattagt aagtgagtta tactaagtcg
    16381 acatttatgt gtcaagacat attaggagag agaaaaaatg agaaaaaatt cagttcgaaa
    16441 actaacgatt tctgctttat tgattgggat gggggtcatt atcccaatgg tcatgcctaa
    16501 aatcatgatt ccaccagctt cctttacttt ggcgtctcat gtgccattat ttattgcgat
    16561 gttttttaca ccaggagttg cagtggctgt tgcgctaggt acgacatttg gtttcttttt
    16621 aacaacgccg gttatcatcg cattgcgagc attgtcacat cttgtgtttg cgttgattgg
    16681 ggcttggtac ttgcaaaagc atccgagtat cgttttaaaa aatggacagt ttacttttgc
    16741 taattggcgt ttccaagggt tcaattttgt gatcggtgtg attcattctt tagcagaaat
    16801 gctagtagtc agcatctttt actttattgg taatatgcct gaaacatatt attctcaagg
    16861 atatttttat acagtattca tcttgatggg gattggtggg cttatccaca gtttagttga
    16921 ttataatatt gcttatttta tagcaggaac actcagcaaa tattttgata tccccgtatt
    16981 tacggcagca aaaaaagaaa atcggaaaga gtcgattcaa gggtacgcga agaaaacgac
    17041 atattagaca tgatctctca aaaaaaaaac tctcatctag taatggataa taaatcctct
    17101 aagatgagag ttttttgtcc taaaaagagc tgcaccaaca tcttctggca cagctctaac
    17161 agggggcaat ttatggaagt ttgatgactt gttctgtttc tttatcaaag aagtggcctt
    17221 tgctgatgtt gaaggcaagg tcgactattt cgcctggttt atggaaatct cttgcatcta
    17281 cttttgagat gaactcagta gaaccagttt ttgtgtaaag catcgtttca gctccaagta
    17341 attcggaaac gacaacttct gatttcacta cggcttcagg tgaagcttca agggctactt
    17401 gttcactgtg gatgtcttct ggacgaatac cgaagataac ttcttttcct tcataacctt
    17461 tttcaactaa tactttgttt tttccttcag gaatacgaag cttaagccca tgtccatctg
    17521 tgataacacc atttttcaat gtcacattaa agaagttcat ggcaggagat ccaataaagc
    17581 cggcaacgaa catattgacg ggtgtatcat aaacttcttt cggtgtaccg atttgttgga
    17641 taaatccgtc tttcatgata acgattcggt cagccatagt cattgcttct gtctgatcgt
    17701 gagtaacata gatggtcgtt gtttcaagac gctggtgaag tttggcaatc tcagcacgca
    17761 tggctacacg taatttagca tctaaatttg ataatggctc atccattaag aagacttttg
    17821 catcccgaac gattgcccgt cccaatgcca cacgttggcg ttgtccacca gataaagcag
    17881 ctggttttcg tttcaagtat tctgtcagac ctaagatctc tgctgcattg tccacacgtt
    17941 ttttgatgtc tgctttatca tatttacgta atttcaagcc aaaagccata ttatcaaaaa
    18001 cagtcatatg aggatacaat gcgtagtttt ggaaaaccat ggcaatatca cggtctttag
    18061 gtgccacgtc attcatgact tttccgccga tatttagttc tccttcagag atatcttcca
    18121 gtcccgcgat cattcgcaaa gtagttgact ttccacaacc ggaaggacca acaaaaacga
    18181 taaattcacg gtctttgatt gatagattaa aatcagttac agaataattt tctgcattgt
    18241 catatttttt atatacgttt ttcagagcca tttctaccat aatacgttcc acactttctt
    18301 ttttagtttt attaactatt taaagtataa atgaaagcgt taactaactt caatgtcatg
    18361 ttgaacaaga attttttctt ttttttgtca atgtgctagt tttaagcttt aataaatatc
    18421 cataagaaat aaaaactgat acactggagt aaataagtat gttaacaata tcaaaaggag
    18481 tgtctcttat gaaaatcgct ttgatcgctc atgaccgaaa aaaacctatg atggtgaaat
    18541 tagcaacagc gtataaaaca atattggaaa aacatgaact ttttgctacc gggacgactg
    18601 ggcagaggat cacggaagca accggcttgc cgatccatcg gtttaaatcc ggaccgttag
    18661 gcggggatca gcaaattgga gccatgattt cggaggatca gttagatttg gtgatttttc
    18721 ttcgagaccc gctggctgca cagccccatg aaccagatgt aacagcactt atccgtttat
    18781 ctgatgtcta tgagattccg ttagctacga atatcggaac agctgaggtg ctactaagag
    18841 gactggaagc cggcttccta gattttcgaa gaatcgtcca tgaaatagac aagcatccac
    18901 tttcattttg atggataagc gtatggaaaa ataacgttta atgttcgcct atatgtagct
    18961 ataaaagcta aacaaactga taatatgcga tataatacga acgtattgga taatataaac
    19021 aataatatta gtttttcgta tttgcagtaa atgtgaaaat atgctatagt ttctgtggga
    19081 aaagatccca aaaataatac agagtagaaa ataaagcgtt aagtgctaag aggatgggat
    19141 gttgcctctt ggacgaagga cgtaatgttc gcggttttat tttcgcattc gctgcatgat
    19201 acgatgtagc agctcttaaa ggagatgcta aaaatgaaga aacaccggtt agacgcacgt
    19261 atgattgcta ttatgggctt attgattgca ttgatggtca ctttgtcccg actggtagca
    19321 atcgagaccc catttattaa aatcagtgtc acatttattc cacaagtgat catgggcatt
    19381 ctttttggtc ctttttggtc agggataggc gcagttttag ctgatttagt aggaatggcc
    19441 ctcttctcga aatctgcatt ctttattgga tttacattga atgcatttat tgaaggagca
    19501 atctacggat tctttttcta tcgaaaagag atcacttgga aaaatgccat tctggctacg
    19561 cttagtgtca cgctgatcat caacttattt ttgacaccat tatggcttgc gttgatgtac
    19621 catgtgccat tgttcagctg ggtcgtttgg gcgccccgcc tgttaaaaac agtaatatgg
    19681 ttaccgattc aatcaattgc catttattac gtaggacgta gtattcctta caaaaaaatt
    19741 ttaagaagtt tagcgatcca cgctaaataa aaaacaagca gtcttcccat cccagaagac
    19801 tgcttgtttt tattgtgctg tgaattgtgc ttgatagagc cgtcggtaat aaccatcttt
    19861 ttgaagcagc tcatcatggg tgcccatttc gacaatagct ccctgatcca tcactaatat
    19921 ctggtccgca tttttgattg tggacaggcg atgggcaatc acaaaactgg tttttccttc
    19981 catcattgct aagaaggctt gttggatttt tttctctgtc aatgtatcga ctgagctggt
    20041 agcttcatct aagatcaaca tagggggatt actgatcatc gttcgagcaa tcgtcaacag
    20101 ttggcgttgt ccatctgata tctttaaccc ttgaccgcca atcttagtat ctaatttttc
    20161 cggcaaacgt tctacaaatt catacatata agaggctttt agggcatcat tgatttctgc
    20221 gtcacttgct tccggattcc caaaacgaag attttctcgc aatgtactgt caaatagcca
    20281 agtatcttgt aacaccattc caaaactttt acgtaatgta tcacgttgga tagagcggat
    20341 gtcatggtca tcgactgtaa ttgttccttt tgttgtgtca taaaaacgca tcagcaaatt
    20401 aacaagtgtg gattttccgg cccctgtttt tcctactata gcaatggttt gacctggata
    20461 agcagtaaaa ttgaaatctt cgatcaaacg ctgcttgggg tcataagaaa aagaaacatg
    20521 atcaaaagag acttttcctt tgatattttt gggttccaag gcataggcat ctggcaaatc
    20581 aggcttttct tctggctgat cgatcaaatc aaatgctcgt tctagaccag aaaaagcggt
    20641 ttgcagttgt gtcgtgattc cagaaagttc gataaatggt ttagaaaact gacttgagta
    20701 gatcgtaaag ctggaaatca caccaacagt gattgacgaa ccactcttca atgccaatag
    20761 gccgccaact aaaccaatcg ataaataagc aaggtgatct acaaaacgag aagacgggtt
    20821 tgtcaaggaa gaagaaaatt gtgcccgttg tcctttttca tatagatcag catttagcag
    20881 ttcaaattgc tgttgactag cttcttcttg ctgaaaagct ttcacgattt tctggttacc
    20941 gattcgttcg gtcacaaagc ccgagatatc cccaataatc ctttgctgtc tatcgaaatt
    21001 tgtttgtgaa gcacgagcaa cgatccaact cacaaggaaa ataattggtg tagaacaaag
    21061 aacgaccaat gtcagaagtg gacttagacg catcataaaa ataaacgaaa gaatgatgat
    21121 tgccactcca gaaaataatt gattgaaggc cgcagaaatt gcaatggaaa tattgtccat
    21181 atcatttgta aatcgactga cgatatttcc gtgggagttt tgatcataat aacgaagtgg
    21241 caatcgattc agatgagcaa atgcatcttt tcttaatttt gcaacggata agtaagctaa
    21301 ccgattgctt aacacttgga tgagccattg actgactacg ttgattaaaa cgataccacc
    21361 gaatagaata agtaaatgga acagtcgatt gaagcggacc tgtccttctc caatcatttg
    21421 atcgatactt tgaccaattt gtaacgtcat cacaactgtt gcaatcccat tgatgattcc
    21481 tagtatcaca gctagccaaa tgtcttttgc ataggcagta aggtaaggaa tgaatcggcg
    21541 gagcgtctgc aaggataatt tttttacttt cataactatt cttcctcctg tgatgcgacg
    21601 atttcctgat attctgctga ttgagcaagt aattcttcat gggtaccctg agcagcaatc
    21661 ttaccttgat ccatcactaa gatatgatgt gcttcttgaa tagagcggac acgttgggag
    21721 ataataatca ctgtactgcc aagataagtc tgtagcgcct tacgcaaatc caagtctgtt
    21781 tgataatcca acgcacttaa agagtcatct aaaatcagta atgctggctt ggcaatcaaa
    21841 gctcttgcaa tcgtcagtcg ctgtttttgt ccgccagaga aatttttccc attttccatg
    21901 acttttgtat ctaaaccatt tggaagctga cggacaaatt cttctgcctg agcgattttc
    21961 agagcttccc aacattcttt ttctgtggcg tctttcttac cccattgaag gttttcacga
    22021 atagtcccgg taaatagtac agaagtttgg ggaacaagag caatttgctg acggagatga
    22081 tccagctgcc aagaacgaac atccattccg tccattataa ctgtaccgga actgacatca
    22141 tagaaacgcg gaatcaattg tgtcaaagtc gttttcccgc ttccggttgg accaatgatt
    22201 ccaaggactg tatttcgctt cagctgaaat gtaatatttt ggagagaaag accagaatca
    22261 ggagtataac ggaagtcaac atggtcaaaa gcgataattg cttcagaaga aaaatcggga
    22321 gtagtactga cctcagcttc aagaatcgaa ttttctgtgt ctagcacctc tttcacccga
    22381 tttcctgaag cttcggcacg tgtgaagatc acgacgaggt ttgaaacaac gatcagtgcc
    22441 aaaagcattt gattcatata attgatcaaa gcaagaactt gtccttgttg caagctacca
    22501 atatttactt tgatcccgcc aactgtcagc aaacagatga tcccgacatt catgatcaag
    22561 gttgtagcag gtgataatag ggcagagata ttggaaacac gtaaataatt gttcgctaga
    22621 tcatttgtgc ttttatcgaa cttctctgtt tctttttttg tctgcgcaaa tgcgcggatg
    22681 acacgaacac cgctaagatt ctgactaacg tgttcattga gctgatctag tttttcttgc
    22741 actttttggt agagaggaac ggtttttttg atgataaaaa ataaaatgat tgagaaaata
    22801 ggcaataatg ctaagaaaaa cagacctact tcccagtcga tcacaaaagc catgaccact
    22861 gacccgatac ttaaaaacgg tgctctgatc aataaacgaa tgaccattgc taaagctagc
    22921 tgaagctgat tgatgtcgtt tgtcattcgg gtgatcaagg tatctgttcc aaaatgattc
    22981 aactcgttgt gagagaaagt attgattttt ttgattagct gattacgtaa ctctgtacca
    23041 aatccttgtg aagctactga agcatagtat tggcaaatga tagcacaaat cagaccaatc
    23101 acagacatag cgaccatcca taagcccatt tcaatgattt ttcctcggtc attcattttt
    23161 agtccattat cgatcaagga agccatcatt aaaggcagga caagttcgaa gacagcttca
    23221 agaaatttga agaacgggcc caaaatgatt tgcttacgat agtcttttgc ataacgtaat
    23281 aactgaatca tatgattcct cctattattt atttgtccac aattgaaaaa acaagtacta
    23341 ccacattgta gcttaggaaa tttagaatga aaagagaaca agccttttca taaatgaaag
    23401 ttttattatg aagaagcttt agaaattgaa tagactattt tttttaaaaa aaagaaaatg
    23461 agaaaacatg ccagtctctt cattcatagg aacgtctatt ttacttaatt tttctataaa
    23521 tgaattctgc ataaaaaata tatcaagtaa atgtttatag atgaaactct atttacttct
    23581 tttttaattt gttcttgcaa tcttttttgc tacgtaattt ttgtaaaaaa gtgggttgag
    23641 agaaatctaa ttgattttca aagaaggagt atgaaaatga gaaaaatatt tattcgttta
    23701 ttgtgggtac tatcttagag gaataaataa tagtggagaa aactatggtt tggcgcagat
    23761 tctatcaggt ttgatggaac gaagacaacc tcccgacatc aagtcaaaag ctacttgaga
    23821 ttttgatctg aaaataaaaa aagaaaacaa ggtaggaaca tcaatgtttc taccttgttt
    23881 tcgtaatcaa tgggaaaaaa ctaagagtgg tgtacgtaat caataaaaag gatggtgaca
    23941 acagtacctc tttactctct gaaagcatca taactcgttt ttcctcatta tgccaataaa
    24001 ttgttgttat accacatata aggatttgta atagctaaaa acagacagga aagttgaaat
    24061 ctgtgaatct tattttgtat ggaaggattt atagaacgca aaaatcaaga taaagatgaa
    24121 taaaaatcct gtttcggcta tatattgcgt atctacgcca ataaaacgag tttgttctgt
    24181 catagccata taaagcaaag gcaaaaatac aactacagcg agataagatc cgatgatttc
    24241 tcgaaaagtt ttccaggaag tatgtttcct gtttttatat ttatccgatt tcattgaata
    24301 gtcatagcct ccttattctt tcatatgttg aaattccagt ataaaaactg gaaggaactg
    24361 tacgtttttt agaataaaaa ttataaggga taagtaggct gctgtctata ataagatata
    24421 tttttcaatt ctttattaaa gattttattg acaaaatgaa tccgttttct atgatggagg
    24481 aggggtgaat aagttgttga agaaggaaat tttatgttac ttagagaacc agacagcctt
    24541 tttcgattta gaacatatga acgaggtctt tacagcacaa aggttagctg atcattttgg
    24601 ggtaaaaaga aacacagtca gtcattattt gaaccagtta aatgatgagc aactactagt
    24661 caaaatcaat tctcgaccag tgatttattt ccacaaagaa gcttttgaaa aacaattttt
    24721 taagttacgc acgaattttt atttttctat cgatgatctc aaagaggaac agcctttttt
    24781 cgcgcaacca aaagacttat tctcttttat gatcggatac gacgccagtt tgaaagaaag
    24841 tatcgaacaa atcaaaacag ctctttacta tccagatggc ggattaccac ttttgattac
    24901 aggcgaaagt ggcacaggta aaagttattt agttaatttg gtctatcagt attgtctgct
    24961 tcatgatctg ctagaagatt ctgcaccatt catcactttt aattgtgcac aatatgcaga
    25021 taatcctgaa cttttgacta gcaatttgtt tggacatgtt aagggagcgt acactggtgc
    25081 agaagaaaat aaaaaagggg cttttgaagc agcagatggt gggatcttgt ttttagacga
    25141 ggtacatcgc ttaacaccag aaggtcagga aaaactgttc acctatcttg atcaaggtgt
    25201 gatttatcgt atgggagaac ctaatttgtc acgccggatc aaaacacgtc tcttttttgc
    25261 tacgactgaa gagattacta gtcatttctt aacaactttc attcgaagaa tacctatccg
    25321 aatcgaacta cctgcactta atcagcgtag tcgtaatgaa cggctagaac tgatttactc
    25381 tttttttctc gaagaacaaa gaaaaatggg gagccctttg tcagtcagtg gaccagcttt
    25441 gtcactttta acaagtcaga aattatccgg aaacatagga gagctgaaaa atatcgtcaa
    25501 agtttcagca gcaaaagcat atgctgaaca gcgtgaacag cctgaaatcc atgtcacgat
    25561 ccatcatttg caaaaagaac tacttgcgca gcctattact aaaaatacag cacagcaagg
    25621 aatctatatt acgcaagacc agaccttaga gcaattaatg gagaagcaac agcccgaaca
    25681 gcagcgaatc gttaaaagtt ttgagcaaat cctaattgat ttcaagaaaa ataactgtct
    25741 gctatattct tgcgaaggaa aattaaagca agaagtgatt caattatttg attttttgct
    25801 gtttgagacc agtagacaag aaaaacacga gctgctggtt tattggacac aatctatccg
    25861 agagaccttt cgacaaatgg aaactgccta tcaaatcaaa ttcgatggaa acagtgtcta
    25921 tgccttgagt tactatctct atcagcgaag cactatcaaa tggttaccgg aagaacatga
    25981 tattatccaa ctaatcaaag aattagaaca acaagtttct cagtcttatc catctagtta
    26041 tcactatgtc caacgaattc tggaattaag taagatcaaa ttagatattg aagacacaag
    26101 tatggatcgg attttactga cgatctatct aaaaaaagcg aaatggtcaa aagaaaaacg
    26161 actgccaaaa gcgatgattg ctgctcacgg ttatgcaact gctagcagta tggcaaatgt
    26221 agtaaatcgt ttattacaag aggatctatt tgaatcattt gatatgccac tagatgtcac
    26281 accacagcag attgcttcag aaatccttga ttattgcgaa aacagcgatg tttcaaatgg
    26341 attgatcata ttagtagaca tgggatctct gaaagaaatc catcagtttt ttaaaaaaca
    26401 gttatccgta ccgcttttga ttttgaataa tgtgacgaca cctttagcaa tcacagtagg
    26461 ggaatgcctg caaaaaaatg tatcattaga agagatagca gaagagactg ttcgacagat
    26521 ccagccagaa tggcgactgc tctatccaga ggaaaacaaa cccaaagcac tcattactac
    26581 ttgcttcact ggaatcggta cggcggtcca cttaagtgaa ctgctagaaa aaagcttacc
    26641 tacggcttgt cagttgaaaa tcatcccata tgaatatcag caattaaaag ataagaaaaa
    26701 cagtgaccca ttattttcca tatacgaagt cgtaggaatg attggcacca cagatcctga
    26761 tatcaccaat atcgactatc tttcattaga agacctcatc tcgggggaaa agatgtctgt
    26821 actagctgaa tggttggaca ctactatgaa tgcttcagag aaggaaatat tcaatcagcg
    26881 catcattcgt aatttttcat tggaaaaagt actggattcc gtgactattc tagatacaga
    26941 gaaggtgatg ggagaaatcg atcattttat gagagaatta gaggtgcagt tgaatcgaag
    27001 catcagcaat cccaaaaaat tagcgttgta tgtccatgtg agctgtctca ttgaacgatt
    27061 gatccgacaa atgccaatcg aaacctatca aggattagag aaatggaagc agtgtcaaaa
    27121 agaagggtta tctgctatta aatctgcgtt tagtgtcatt gaagagcatt atagtgtcat
    27181 gatccccaat tctgagattg cctatatcta tgatattttg agtgaagata cagagtttct
    27241 ctcgatagaa gaagaatttt gaacataagc aagttgttgc cactaatttt ggcatgcaac
    27301 ttgctttttt tagtatgtgt aagtgaaaag aggagatgaa aaagtggata gagaaattat
    27361 cttagcttcc catggaaaat ttgcttcagg gatattagat tcattagagc tgatctatgg
    27421 gaaaaatcat tctattactg tattcgattg ctatactgat gagaattttg atttagcgga
    27481 aacagtaagg cagcttttta tgaggtacaa agataaagaa atcattgttc tgacggatct
    27541 ttttggcgga agtgtcaaca acgaattctt acaatatatc aatcgggatc atgtctatct
    27601 cgttgccgga ttgaatttgc cactattgat tgagctagta tctcggttgg acgctgaagt
    27661 agagacaact gttttgatcc agcaggtgct aactgattca aaaaaaatga tccagttttg
    27721 taacgagcgg ctcaatcaga aaatagaaga agacgaattt taggaggaag aaaaatgatt
    27781 gtattgacaa gagtcgacca tcggttatta cacggacaag tagctttttc atggacacaa
    27841 acaataggcg cagactgtat tttgattgct aatgatgatg tacctacgaa tgagatacga
    27901 aagacaacga tcaaattagc caagccacaa ggagtcaaac tagtgatcaa atcaattgat
    27961 gactcgattg ccgcgttgaa aagcggggtg acagataaat ataagttatt catcgtagtg
    28021 gaatcgatcg aggatgcata taagctggca gaagcgtatc cagaaatcaa aaccatcaac
    28081 ttaggaggga tcaaggcaaa acctggtact cgcagcatcg gaaaagcggt aaatatttta
    28141 ccagaagagg aggaacgctt gaaacgatta attgacaaac aaatagaagt agaaatccgt
    28201 caagtaccag cagataaaaa agtggatgta agagaagttt tgtaagaaag gaagaacctc
    28261 aatgattgta caagcagtct tattaggaat tgttgcattt gtcgcacaat cagaatatgc
    28321 actagggaca tcgttgcttt ctcgtccgat tgtgacgggg ctgtttacag gaatcgtgtt
    28381 aggtgatgta aaaactggga tcattatggg cgcaacttta gagttagcat ttatcggatc
    28441 gttctcagta ggtgcttcga ttccacctga tgttgtgacc ggtgggattt tagggacggc
    28501 ttttgctatt acagctggag ccggtacaga aaccgcgctt ttactaggtt tgccgattgc
    28561 tacgttgaca ttgatattga aaaatattta cttagggctt ttgattccaa ttatgaacca
    28621 taaagcagac aattatgcag aagaaggaaa ttataaagga gtcgagcata tgcacttgct
    28681 tgccggtatc ggtctttcct tgatgcttgg tgttgtggta atggtttctt atatggtggg
    28741 aagcaatacg atcggaaatc tattaaatat gattcctgac ttcgttcaaa aggggttgtc
    28801 agtagctaca gggttgattc cagctcttgg ttttgcgatg cttgctcgtt tgctgatcaa
    28861 caaacaagtt gcaccatatt ttttcttagg ttttgctata gcttcttact tagagattcc
    28921 tgtgaccgga gtagctattt ttggtgcgat tttagcagtt gtggtagtca atattatgaa
    28981 tgtccgtcaa ctaccacttc aaacaactgc aggggaggta gaagaagatg aagaattctg
    29041 agatcaaaaa cagcaaaata acgaaaaaag agctaaatca cgtattttgg cgttcttttc
    29101 aaatggagtt ttcttggaat tatgaacgtc agatgaatat ggcttatgtt tatgcaatga
    29161 ttccgatttt gaaaaagtta tatcagtcga aagaagaaat ggcagcagct ttgaaacggc
    29221 atctagagtt ttttaacacg accccacata tcgttacact gattttagga atcacggctg
    29281 caatggagga agaaaataag gatgatcctg aatttgatgt gactgcgatc gatagtatca
    29341 aaacatcttt gatgggacct cttgcagggt taggagattc tttcttttgg ggaactttgc
    29401 gattgattgc cacaggtgtg ggtacctctt tagccatgca aggaaatatt ttaggcccta
    29461 tcttgttcat tttgattttc aatattccgc acattctgtt tcgctatcta gcaacaggtt
    29521 ggggatatcg cttaggaacg ggttttttga aaaagatcca ggcaaacggt atgatggaaa
    29581 gtttgacatt aggcgcgtct attattggac tgatggtcgt tggagcaatg actgccacga
    29641 tgatcaccat caatattcct ttaaaaatcg gttcaggaga aaatgctgtg actgttcaaa
    29701 gtatttttga tgatatcgtt ccaaatatct tatgtcttgg agcatttggc atagtgtttt
    29761 atctattgaa gaaagaagtg aaaccattaa cgatcttgat cggactagct ttctttggaa
    29821 tttttggttc gtggataggg attttttaaa aaaggagcag agaaaatgtt tacgatgcaa
    29881 gattatatct atgaagaaaa ggaagtacta agtacgatct tgaaaaagaa tgatttttcg
    29941 acaagagaac atatgaaaaa aacagtgaat ttactgatct tagcaacggg ttcatcttat
    30001 aacgcttgtt tagcagcgaa gccagcattg gaatcttatg gtgatcttac tgtcgatatt
    30061 caagaacctt tttatttttt caattatgga aaactttctc cttcaatcga tactgtgatt
    30121 gctgtgtcac aaagcgggaa aagtgcttct actgttgaag cagtaaaaat gatacaaaaa
    30181 caagggttac ctgttgttgc tatcacaaat gacgtgcaaa gtccacttgc tttagaagct
    30241 gcacaaatta ttgatttagg tgccggggta gaatcagtag gcttcgttac aaaaggctat
    30301 tctgcgactg ttttacagtt actactctta gggataggaa ttggaataag taaaaataaa
    30361 atcagcaaaa aaatagaaca agactacatg caacagttgc gcaaaataat caaccattta
    30421 ccagcgatta ttcaaaaaac ggaagaattt tttgatggat atcaaagttt atttcgttta
    30481 gcacagcgtt tcgtcaccat tggatatggt ccaaactggg gaacagctaa agaagcagag
    30541 acgaaattga ctgaaacaat ccgcgttccg tcacaaggat ttgagttaga agcttatatg
    30601 cacggcccat acctggaggc agatgcttcg catttgctat tctttattga aggggaaagt
    30661 gtaaataagg aacgttcgca aaagttgcaa agatatatga gtcggtacgt tggagagact
    30721 ctcaccatta ccacaaaaaa agcaagaaac gagaaaacac ttggattagc aatagaatgt
    30781 gatgaatatc tgtctgtatt agcgcttgtc gttccgtttc aactattcgc ttataaaacc
    30841 gcagtagcta aaggtattga cttgaacaaa aagatttttg aagactttga tacagtcttg
    30901 aaaagtaaat tataagaggt ggaaatcatg ctgaaattca atgaagaaga acaaatcaaa
    30961 gcaataaaag gcgcactagc tttacgtccg caagtagaag aaatcatcga taaactttat
    31021 gcggaaaaat ttgacgctgt ctattatcta ggaatcggtg gaacgtatgc ttcctcgatg
    31081 caagctgtca cttatatgaa tgggaaaagc aacttgcctg tctttgtaca gcatgctgca
    31141 gaatattaca ctactggaaa caaaagatta acaaaagatt ctatcgttat tttgtcctct
    31201 gttaccggta caactcaaga agtagtgaaa gcagtagaac aaatcaagaa agtaggagcg
    31261 acactgattg gatttatcga taaagcgaac agtaagttgt ctcagctatg cgattttgtg
    31321 gtcacttatc cagctcctgg aacagaacaa atcaaatttt ttatggcagc agatcgattg
    31381 atgtatttga atggagaatt cgaagcttat tcagaatact atgaacagct cgaacgttat
    31441 ttaccaacag ggttagtgga agcagagaaa aaagcagatg catttggctt atcatttgca
    31501 gaaaaacatc gacgtgatag tatgcattat tttattggag cagggaatca atggggagct
    31561 gtttattcat atgccatgtg ttactgggaa gaacaaagct ggcttccatc gaaatctatc
    31621 catgctgccg agtttcttca cgggacgtta gagatcgtag aagaaacaac cccagtcaca
    31681 ttatttttag gcgaagatga acagagagtg ttatctgaac gtgtggctaa acttttgcca
    31741 aaaatttgca gcaattatac attgatagac acaaaagact atccagtaga aggtatcagt
    31801 gaaaaatatc gcggacgtgt tttgtctttc ttattgatgc atgtagtgac acaacgcatc
    31861 gatgctcatg tggagcagtt gaactgccat ccattagaga tacgccgcta ctatcgtcag
    31921 tttgattatt aggatagact ggataaaaag aatataaaaa ataaagagag cagcgataac
    31981 tgctcccttt atttttttaa aatattggca tcaattacgt tataaaacac attttttgca
    32041 ttatctacag ttcaacttat aatctgttta atgggacatg tgggtgccaa ccattttttg
    32101 ttatgcaata gtactattta gttgttgcat ggatccgtag taaaagtcca agtacaacga
    32161 gttgcgccag tttacatagc gttttgtatc cagaatgttg actaaaacaa aagttcctat
    32221 gtcaaccgtc aacagagaca atctgtccgt tgcaggacct gacagattta gaaatgttca
    32281 atctgtctct tatgtatttt aaatcaatac tcactaggag aagtttccta acctcctaat
    32341 agtaatgctg gtagtaacat tgtcaaaact gcttcctctt aggaacaagc aaagcataac
    32401 gagtgtaaaa ataaaatgta gactattgta acatatcatt gattatttca agtaaaaacg
    32461 ataaaagaag taaacaaaca cttaaaaaca atggaaatta gtcccaactt taagaaatgt
    32521 atactataat ggaaattatc aattatttat ttggaggaat atccatgaaa caaaaaatcg
    32581 cagttcttgg tcctggatct tggggcactg ctttagcaca aacattggca gaaaacgggc
    32641 atgatgtccg tatttgggga aatgtacctg aacagattga tgaaatcaat aagtatcata
    32701 caaatcagca ttttttacct gatttgacga tcccagaatc gattatcgga tataaagaat
    32761 tggcagaagc agtggataac gcagatgcta ttttatttgt tgttccaaca aaagcgattc
    32821 gttcagtcgc acaggaactt attggtaaaa tgaatactaa gccaatcatt atacatgcaa
    32881 gtaaaggact agaacaagat acgcataaac gaatctcaga agttttggct gaagaaattc
    32941 cagaagaaaa aagacaggca attgttgtat tatctggtcc tagccatgct gaggaagtgg
    33001 cagtacatga tatcacaaca attacagctg caagtattga tcaaaaagca gcagcctatg
    33061 tacaaaaact gttcatgaat gattatttca ggatttatac aaataatgat gtgattggcg
    33121 tagaaactgg tgcagcactg aaaaatatca ttgcaattgg tgccggcgct atccatggat
    33181 taggattcgg agatgatgca aaagcagcaa tcatgactcg aggattggca gaaatcagcc
    33241 gtcttggtgt cgcgatgggt gccaatcctc tgacatttat cggtttgagt ggtgtggggg
    33301 acttgatcgt tacttgtaca agtgttcatt ctcgcaattg gcgtgccggt aacttgttag
    33361 gacagggaca taagctggaa gaagttctag aaaacatggg aatggtcgtt gagggtgtgg
    33421 caacgacaaa agcagcagta gagctagctc aacagctagg tgttgagatg ccaattactc
    33481 aaacaatcta taatgttttg tataatggag aagatatcaa aaaagcagca aaagaaatca
    33541 tgttacgtga cggaaaaatg gagaatgaat tttctcttcg ttaaatagat gatcaatagt
    33601 taatcgt
//
LOCUS       DACDPS010000023        32535 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.023, whole genome shotgun sequence.
ACCESSION   DACDPS010000023 DACDPS010000000
VERSION     DACDPS010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32535
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.023"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            133..507
                     /locus_tag="IWJ07_001156"
     CDS             133..507
                     /locus_tag="IWJ07_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP9332749.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(621..1250)
                     /locus_tag="IWJ07_001157"
     CDS             complement(621..1250)
                     /locus_tag="IWJ07_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9332750.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1384..1456)
                     /locus_tag="IWJ07_001158"
     tRNA            complement(1384..1456)
                     /locus_tag="IWJ07_001158"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1421..1423),aa:Thr,seq:ggt)
     gene            1670..1743
                     /locus_tag="IWJ07_001159"
     tRNA            1670..1743
                     /locus_tag="IWJ07_001159"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1704..1706,aa:Asn,seq:gtt)
     gene            1698..1847
                     /locus_tag="IWJ07_001160"
     CDS             1698..1847
                     /locus_tag="IWJ07_001160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332751.1"
                     /translation="MTVNHDVVGSSPTAGVNKAYAKKKKSNHLGFFFFYYYDESSFLF
                     LSRML"
     gene            2340..3668
                     /locus_tag="IWJ07_001161"
     CDS             2340..3668
                     /locus_tag="IWJ07_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP9332752.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3870..4703
                     /locus_tag="IWJ07_001162"
     CDS             3870..4703
                     /locus_tag="IWJ07_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAP9332753.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4719..5456
                     /locus_tag="IWJ07_001163"
     CDS             4719..5456
                     /locus_tag="IWJ07_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAP9332754.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5611..6579)
                     /locus_tag="IWJ07_001164"
     CDS             complement(5611..6579)
                     /locus_tag="IWJ07_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAP9332755.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELKKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6811..7644
                     /locus_tag="IWJ07_001165"
     CDS             6811..7644
                     /locus_tag="IWJ07_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAP9332756.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7678..8517
                     /locus_tag="IWJ07_001166"
     CDS             7678..8517
                     /locus_tag="IWJ07_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9332757.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8543..9103
                     /locus_tag="IWJ07_001167"
     CDS             8543..9103
                     /locus_tag="IWJ07_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP9332758.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9302..11023
                     /locus_tag="IWJ07_001168"
     CDS             9302..11023
                     /locus_tag="IWJ07_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAP9332759.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11188..12330
                     /locus_tag="IWJ07_001169"
     CDS             11188..12330
                     /locus_tag="IWJ07_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP9332760.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12346..13557
                     /gene="thiI"
                     /locus_tag="IWJ07_001170"
     CDS             12346..13557
                     /gene="thiI"
                     /locus_tag="IWJ07_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAP9332761.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            14130..14777
                     /locus_tag="IWJ07_001171"
     CDS             14130..14777
                     /locus_tag="IWJ07_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP9332762.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            15170..17815
                     /locus_tag="IWJ07_001172"
     CDS             15170..17815
                     /locus_tag="IWJ07_001172"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAP9332763.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            18125..19438
                     /locus_tag="IWJ07_001173"
     CDS             18125..19438
                     /locus_tag="IWJ07_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAP9332764.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            19428..20081
                     /locus_tag="IWJ07_001174"
     CDS             19428..20081
                     /locus_tag="IWJ07_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9332765.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            20136..20828
                     /locus_tag="IWJ07_001175"
     CDS             20136..20828
                     /locus_tag="IWJ07_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAP9332766.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            20978..21178
                     /locus_tag="IWJ07_001176"
     CDS             20978..21178
                     /locus_tag="IWJ07_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9332767.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(21432..23111)
                     /locus_tag="IWJ07_001177"
     CDS             complement(21432..23111)
                     /locus_tag="IWJ07_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP9332768.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(23113..23325)
                     /locus_tag="IWJ07_001178"
     CDS             complement(23113..23325)
                     /locus_tag="IWJ07_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAP9332769.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            23718..25448
                     /gene="efrA"
                     /locus_tag="IWJ07_001179"
     CDS             23718..25448
                     /gene="efrA"
                     /locus_tag="IWJ07_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAP9332770.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            25445..27205
                     /gene="efrB"
                     /locus_tag="IWJ07_001180"
     CDS             25445..27205
                     /gene="efrB"
                     /locus_tag="IWJ07_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAP9332771.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            27300..27497
                     /locus_tag="IWJ07_001181"
     CDS             27300..27497
                     /locus_tag="IWJ07_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332772.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            27650..28135
                     /locus_tag="IWJ07_001182"
     CDS             27650..28135
                     /locus_tag="IWJ07_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAP9332773.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(28239..28832)
                     /gene="clpP"
                     /locus_tag="IWJ07_001183"
     CDS             complement(28239..28832)
                     /gene="clpP"
                     /locus_tag="IWJ07_001183"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAP9332774.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            29012..29539
                     /locus_tag="IWJ07_001184"
     CDS             29012..29539
                     /locus_tag="IWJ07_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAP9332775.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            29630..30367
                     /locus_tag="IWJ07_001185"
     CDS             29630..30367
                     /locus_tag="IWJ07_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP9332776.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            30371..31288
                     /gene="miaA"
                     /locus_tag="IWJ07_001186"
     CDS             30371..31288
                     /gene="miaA"
                     /locus_tag="IWJ07_001186"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAP9332777.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            31290..32519
                     /gene="hflX"
                     /locus_tag="IWJ07_001187"
     CDS             31290..32519
                     /gene="hflX"
                     /locus_tag="IWJ07_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAP9332778.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT        10772 a         5522 c         6875 g         9366 t
ORIGIN      
        1 gtctacactc tgagatgtaa gaaatatcat aagatatttc ttacatcttt ttgttttgag
       61 caggacttta gataatttta ttaatatatg ctagcatgct tttgtaagaa aaagaaagag
      121 ggcgtattag ttatgtatct taaaattacg gaacaagcac aagaaagatt acaaaaatat
      181 attgatgaag gcgcaacagt tatattggat ttagatgatg gcgttgggga atattctaaa
      241 atgggtgttt gctctttaga tactagtttt cgtttacttc ttcttgataa agaacagaga
      301 aaaaatgatt ataaactcca attagatagt gatattgggg atgtttatat aaaagattat
      361 tcaaaaatgt acatggatga acatatgacc ctttctttag atccacgtct aggtgtattt
      421 actcttgaaa gcccaagtgg ttctttagac agtcatgttc aattagtaga tctacgacca
      481 gaaaaattct ctggaataaa acaataactt tctctctatt gtccataaaa ctagtctcaa
      541 tcaagctgta ttgaaaaaac aagacaacat tcttaccatg gtaaataaaa agacgacaca
      601 ttttgtcgtc tttttttaaa ttaactattt tcgataaaag atcgcatagc ttgtgccatt
      661 atttctggct tttctctatt caattcatgt tttccgtttt taataaagag tagccggcca
      721 ttcgttgaaa gtttatataa acgttttgct gctggtttat tcgcatgatc tttggtaccg
      781 cataaaatca atttaggaag tcgtgatgta cgaagctgtt ttttgcagtc taatgtttca
      841 agtgaacgaa gtagggtaag ggtttgtttt ttagatagac ctaatttttc aaatatctta
      901 gaaggtacgc agtaaaaata aatattctgg aaagtaagca ataagcgatt aggagactca
      961 aattgtggag cgactaaaac aacctttttt aaatatggag aaggagttgc aagatagttt
     1021 aaagcaataa cagcgcctaa agataatcca cataaaataa aaggttcctt tatctgattc
     1081 aattgtactt cgatgtattt ggttaagtcg tctaaagtag cagtgtctgt gctgtttaaa
     1141 tgattgaaca atgaaagact tttgcaagaa taactattgg gtagataaga gattacatct
     1201 ttccatgatt gttcgtcttg gcctagacca tgcaaaaaaa caattttcat attgctctcc
     1261 tttttatagt tatcatatag actttctttg tactattaaa atataaacat tcatttctta
     1321 cgtcataaaa taaaaaaaca gcgcagacac attgttaaac caacatgttg cactgtttaa
     1381 ggaagccact tgccgggctt gaaccggcga cctcttcctt accatggaag cgctctacca
     1441 gctgagctaa agcggcatta tcattaatac aaataatagg ataacgaata ttaatgtatt
     1501 tgtcaacaga attttttcga aaaaatgtgt ttctctaata ggaaaacaga ttttagaaaa
     1561 aataaaaata acaaaaaatg cgaacggttt ctttgataat ataataaaaa aattcaaaaa
     1621 aagtattgcg ttatttagaa gaaattgata ttatattcat gtacttgatt ccggcatagc
     1681 tcagttggta gtagcgcatg actgttaatc atgatgtcgt aggttcgagt cctactgccg
     1741 gagtaaataa agcttatgca aagaaaaaga aatccaatca cttgggtttc tttttctttt
     1801 attattatga tgaatcaagc tttctgtttt tgtcacgtat gttgtaatct ttacgaaaga
     1861 gtagagttgg ctactcataa tcaaccatac ttcccatcat cgctaaatac gaaataaatc
     1921 agcattccta caccaatcaa agcacaagct gcaccaagaa aagggatgac attcccaaag
     1981 ttatctgtat tcataagtat gccaccaccc acaaacaaaa agccgccagc aatcaggttt
     2041 ttcattttat tcatctcctt tcttacgtta tagtatttcc ttctttcaat gagtattttg
     2101 aaagctatct tattgtatga tgttcttcta ggcctcttct ttattataca ggccattttc
     2161 gttatctcat agaaatattc ctttttatct gaaaactatt gtataccgta tgacattttt
     2221 cttcatgaag ttgtaagtat attctcattc aaatgaaaat ttcatcatag aagagattgt
     2281 atttctaaat tcttaagagt aaaatacgaa gtataacttg gatttggaag gatgattgga
     2341 tgaaagctat tacgcctgat atgacaacaa aatttaacaa acagtttcaa gcaaatatca
     2401 aacagcaggc attgcagcgg gcagttgtga agaacggaat tactgcttct gctgaaaatg
     2461 tttcagcaca tgtggagaat acaccggtat tttctataga tttggctacg gggaaagtgg
     2521 ctaatcaaat gcaatcgggg cgttgctgga tgtttgctgc actgaacaca ttccgtcata
     2581 agatgctgaa tcaattccat ttaaaagagt ttgaattatc acaaaattac acttttttct
     2641 gggataaata tgaaaaggca aattatttct atgaaaacat cattgcaaca ggaaaagagg
     2701 caatcgatag ccggaaagta gctttcttac tcgctactcc tcaacaagat ggcggccaat
     2761 gggatatgat cgtttcatta ttccaaaaat atggcgttgt gcctaaatcg gtgatgccgg
     2821 aaagtgcgaa tagttcaaat tctcgagatt tgaataacta cttgaacaaa ttactgcgta
     2881 aagatgcagt agtgttacgg aaaatggttg ctcaaggaga aacattagac gctattgaag
     2941 aaaagaaaga agaaatgcta gaaagcattt ataacttctt agctatctca ttaggaacac
     3001 ctccaaaaga attcgatttt gagtaccgcg atgaagaaaa gaactatcat cttgaccgag
     3061 gactgacacc acaaattttt tatgataaat acatcggtgt aaaattagat gattatgtta
     3121 gcgtgatcaa tgcaccaaca aaagacaaac cattcaatcg ttcttataca gtagagatgc
     3181 taggaaatgt cgttggcggg aaagaagtca aatatctgaa tgttgatatg gaaactttca
     3241 aaaaattagc gattgctcag cttgaagaag gagaatctgt ttggtttggt tgtgatgtag
     3301 gtcaatcttc tactagaact agtggcatca tggccttaga tgcttattca atggatgatc
     3361 tatttgatac ggattttaca atgactaaag ctgaacgttt agattttgga gaaagtctga
     3421 tgacccatgc gatggtattg accggtgttg acttagtaga cgggcaatca acaaaatgga
     3481 aagtcgaaaa tagctggggc gaaaaagttg gaaagaatgg cttctttgtt atgagtgatg
     3541 cctggatgga cgaatatacg taccaaatcg ttgtaagaaa agatttattg acgaaagagc
     3601 aattaaatgc gttcaatgaa gaaccaattg ttctatctcc gtgggatcca atgggtgctt
     3661 tagcatagac gagtatttca atgaagagta tgtagtaaag ataaaaagag ctggcttaag
     3721 ccagctcttt ttatctttaa agtccattcc ctgtttttta tggtccctgt gctataattc
     3781 taaataaagg ataaggattg gaaataatcc ttttatcatt aaaaatatga tgacgaagta
     3841 gaataaaatt gggagtaggg gggaaagtca tggatatcca aactgtttta tctgatctga
     3901 aattaaatgg taaagtgatt ccagttgttg gtggcgatgt taatcagacc tatcgtataa
     3961 aaaccgaaca tcgtgcttat tttttaaaaa tccatccgaa tgtaaaaaaa ggattttttg
     4021 aagcagaagt agacggatta aaagaactgt cagcattcgt acgtgtaccg gatacgtata
     4081 tgctaggtga aacaagtgaa ggcgcttatt tgttaatgga atggatcgag ccgggaaaag
     4141 gggatcaacg agatcttgct gcagctttag ctaatctcca tcagcaaaca gcgccacaat
     4201 ttggttttcg taaagataac tatttgggta cacttgttca gaaaaattct tttgaagaag
     4261 attggtggac ttttttcttt aaagatcggc ttgaatccca gatttctttg gcagaagaaa
     4321 cgaatcgatg gaatgtacag cgtcaagaaa agtatctgcg atttaaagaa cgtgtattga
     4381 aatcggttga accaaaaaag atcactccac gcctcttgca cggagacctg tggagtggta
     4441 atgtgttctt tgaccagcaa gggcatccag tttttgtcga cccagctgtt tcttatggga
     4501 atcgggaaca agatattgca atgagccagt tattcggtgg tttccgaccg gaatttttag
     4561 atgcttatca aactatattt ccattagaaa agggatggaa agaccgactg ccgatttacc
     4621 agttatatta tcttttggca catttgaata tgtttggaga aagttacggt tcccaagtag
     4681 atcaattact agaaaatttt taagaggagg gcgtgttttt ggcaaaaaaa tataccaaaa
     4741 agcatgaagt ttcatattat gagtgtgaca tcaaccaaac gatgacattt ccttcgatgt
     4801 taggagtagt tatcaaaacg tctgaagatc aaagcgacca gttgggaaga ggcagtgaat
     4861 tcgtcaatag ctttggactt acttgggtca tcacgaatta ttccatggag attaatcgtc
     4921 tccctagagt cggtgaagta atctcagtaa cgactcaagc catggagtac aataagtttt
     4981 tctgttatcg gaacttctgg atccatgatg aagatgggaa agaattagtc aaaatagaat
     5041 ctgttttcgt attgatggat tttgtgaatc gaaaaatgag cagtgttaat gaagagatca
     5101 ttgctccttt tgagagtgag aagatcaaga aaatcaagcg tcaggaaaaa attgaaaaga
     5161 ttgattcggg cttcatgttg ccatatcgtg ttcgttttta tgatatcgat agtaatcagc
     5221 atgttaataa tgcgatgtat tttaattgga tcattgacgt attaggatat gactttttga
     5281 ctacgcatgt tccagaaaaa gtaattattc gatttgataa agaagtagag tacgggaatg
     5341 agatcgaaag ccattttgaa caaatcccta tcgaaaatgg tgtcaaaaca aaacatgaaa
     5401 tccgaatgca agaacagctg tattgtgaag caaatattat atggaaaaat aaataaaaaa
     5461 gaggttgtga cagaaggggg caacttcaag aaataagaag aaatttacga aaattgtttc
     5521 tcaaattttt gtaaattttc gacttagttt tcgaagaagc tatttttgtc acagcctctc
     5581 tttttaagtt tagctattag ataatgatgt ttagttttgg ataaacgaaa tcaattcttc
     5641 acccgacaaa ccatagttta cagcaatcaa tagttttaaa cgtgcttttt gactattaat
     5701 actgttacag aaaataatcc ccattttttt tagttctttt cccccgcctt cataatcata
     5761 aacaggctct gcgattccat tgaaacaacg agaaacaagt actacaggga ttttttggac
     5821 aagaagcttt tccaaagcag ctaaggcttg tggagggaga ttccccgcac ctaaagcttc
     5881 gattaccaaa ccgtctactt tcgtatgggc gatggcttcc aataagtccc cttgcatccc
     5941 agcatatgct ttaacgatcg ggatagtccc atctacagcg gaaatgtcca gtcgctttgt
     6001 ttctaaaagt tcctgcaaga ataaaattcg attcttagtt actaagccga tgggacccaa
     6061 tgtaggtgtt cggaaagtag caacatttgt ggtatgggtc tttgtcacgt agcgtgcaga
     6121 atggatctca tcgttcatga ccaccaaaac ccccttatct agcgcttctt cacaacttgc
     6181 gacacgaatc gcactttcaa aattataaag gccgtcactt ccaagttcat tacttgaacg
     6241 cattgcccca gtcaaaacaa ttggtaattt gttgccaatc gttgtatcaa gaaaaaaagc
     6301 agtttcttct aatgtatctg taccgtgtgt gatcaccact ccggatatac ctgatgcata
     6361 agcggacaag atacgttctt ttaataccaa catgtgagaa ggcatgatgt gtggtgaggg
     6421 aagattgaag atatcttcaa ctaataattc tacatgcttg gggattgtga tttctgcatc
     6481 taataatggg tttgccacat caggactaac accgcccgtg agcatgtttt ctttcatggc
     6541 gatcgttccg ccggtgtgta gtacaagtat ttttttcatt ttacagactt ccttacgaat
     6601 aattttgcat agccgttaaa tcttattgta tactatataa aagagttttg ctatcagtga
     6661 agaataacga atagcctaac ttatcataaa aaagctgacc ttattaaaat ggaaaagact
     6721 caaccttgac ctcctaatac aatggagtat ctggttgagt ttcttttaca agaaagcagc
     6781 caaggaaatc aatcacgaag gtggccggaa atgatcagaa tcggattaac atcgtttaaa
     6841 gaacatgaca aattaactgg taaaaagaat aacactcttt atgaatatgc agggtttcta
     6901 cccttagtgg aattagatac tgcctattat ggaattcccc gaaagtcctc agttgagaat
     6961 tggataagtg aagtacctga atcatttcgc tttgtagtca aagtctatca aggaatcagc
     7021 ggtcaaggag actggaagcc ttattatcag accgaagaag agatgattgc gcatttttta
     7081 gatgttatgt cgccaatggt agatgctgga aagatgtttt gctttttagt ccaattcccc
     7141 tcatcattca aatgtacgaa agaaaatgtc cagtacttgc gcaaacttag aaagtggttt
     7201 gagggttatc ctgtttctat agaattgcgg gattattctt ggtacagcga tgaatttctt
     7261 gaacgtacaa gaaactttat gtctagagcc gctttttctt tagccatcat cgatgagcca
     7321 cagttgctga atacaacgat tccttttgat ccatttgtca ccaatccgaa ctttgctctt
     7381 tttcgattcc atggtaggaa tgcgcaagga tggcaagcaa atgataaaga gtggcgtaaa
     7441 aaaagaacgt tatatcgtta ttcagaaaaa gaactgcaag aactagcaca tgaagtcaaa
     7501 gtagttgatt ctaagacaaa agaggtaggt gtgattttta ataataattc tggtggtgat
     7561 gcggctgaaa atgcattagc actgcaaaaa atattaaata tcaagccaga tggactcaat
     7621 ccaacccaaa tggatctgtt ttaagggctt attttattca agaaaggggc gaagtctatg
     7681 atcaaagcta ttttttttga tattgacgga acattggtca ccaatcgttc aaaagcgttg
     7741 gaatcaacaa aacaagcaat tgaatatgct cggaaaaacg gcattttatg cggtgtagca
     7801 acggggcgca gcccagtaaa gatcaaagaa atcattgatg aattggaatt agatatgtat
     7861 gtcgtgtata atggtcaact ggtttttact gcagatcgca cgattatcga ccaccctttt
     7921 gaacaaaaag tattggaaca tattgttgaa tttgcagatg agaaccatcg acaaatcgtt
     7981 tttggtgcga gaaatcggtt agatggatca acgacgatgc tattaggaca atcgattttt
     8041 atcaagcgac tagtcagctt tttaccgcgt aaattcccgg tacgtctgat gaaaaaaatc
     8101 ctccaggttt ttagccctca ccgacaaaag gatcgttatg aaacgttaga gatattaaaa
     8161 gaaccaattt atcaatgtat attgctcagt ccggagtcag aacagaaaaa gttagaacat
     8221 cgctttcccg agtgtacgtt tcagcgttca aatacgtata ctgtagatat catcccaaaa
     8281 ggtggttcaa aattactagg catccaagct ttcgcaaatg ctgtcgaaat tgaaatggaa
     8341 gaaattatgg cattcggcga tcattacaat gatatcgaga tgctgaaagg tgtaggaatt
     8401 ggagtggcca tggggaatgc tcagatcgaa gtaaaacatg ctgcagactt tgtgactcaa
     8461 tcaaatgaag aagatgggat atacgaagga ctgaaacact tcggtctgat ttattagtca
     8521 atgaaagaag gaaaaaatca gtatggaagc agagacacct tttcaaaaag tagaaacatt
     8581 ccacaaaact tttgatccaa gaagaccaga gatacccacg gcttttacag aaaatgaagc
     8641 tcatacacgt gctgcgttca aaatagaaga acttgttgaa tttgttgcag ctacttcacc
     8701 taataaagaa caactgcatg aagctgttat gctgctgcac gaagcattag ataaagcaga
     8761 agaaaaaata cttggtaaaa cacagtgggg aagtgatccg ctggttgaac aagtagatgc
     8821 actgacagat atcctttact ttacttatgg ttctttttca ttactaggaa tagtgcctga
     8881 tcgaatattc gaaatcgtcc atcaggcaaa tatggggaaa ttatttccag atggaaaacc
     8941 tcattatgac cctgtaacaa ataaagtgtt gaaacctgaa aactggcaga tggaatatgc
     9001 tccagaacct aaaatcgcgg cagaaatcca acgtcagatt caagaagcga ttgctaagaa
     9061 gaaatccgct gatataggca tgtgcacaaa tgaaaaagag tgatcagagt ttcactattt
     9121 ttgttatctt tatacgatgg aaaaacaaaa gattttcgtt taagttagtg aatgcatgaa
     9181 aatagtatgc tataatctag aatagatgtc ttttgcacga gagacgtatt atttggattt
     9241 atgccaagca gaacgccatc tgttgcataa aagatgcata ctgaaaacgg aggaaccata
     9301 aatgggaaat aatgttatca tcggaatcgt cattgcaatc attatagtcg ccattgtcgt
     9361 ctttttggtt agttacttga tgaagaaaaa aaaccaagat cgattggatg ccttagaaaa
     9421 acgcaaggaa gaattatttg acttgccagt gattgaagaa gtcgatgatg ttaaaaaaat
     9481 gcatctggtt gggcaaagcc aaaatacctt tcgtgaatgg agtcaaaaat ggaatgacat
     9541 atctacttcc tcgttcgctg aactagaaag tcaaattttc gaagtggaaa acctcaatga
     9601 aagttttcgg ttcttaaaag gaaaaaaagc agttgttgaa gcagaagcta caatggacca
     9661 aatggagaaa caagtgacgg aaatccgtga aggtctaaag gaattgcggg aaacggaaga
     9721 acgcaactca ctagctgttc agcaggcact tgacgtctat gaagaactga aagaaaaagt
     9781 cagagaagac gagcatgaat acggaccagc tctttttgaa ttgcaaaaac agatcaaagt
     9841 gatcgaaaaa gaatttacgc agtttgtgac tttaaacact tctggagacc ctgttgaagc
     9901 ccgcgaggtt ttggataaag cagaaaaaca tacgtatgaa gtggaagctc tgatgaaaaa
     9961 aattccaccg ctgtatgagg agctccatac cactttccct gaacaacttg aagaaatcag
    10021 tgatacttac gagaaattga tggaagaaca atatgtattc ccagaagaag atcttgctga
    10081 agatatcgca aaagtaagtc gaagaattga aaattcatta gccaatcttg aaaaaacaga
    10141 agttgaaacg gtcgaatttg aaaaccgtga aacagctgat ttgattgatt cgttatatga
    10201 tatcttagaa cgggaaatgg aagctcagag atatgtaaaa actaaccaat caacgattgc
    10261 agagtatatc aaacatacaa caaagaataa ccgtcaatta ttgattgagc tggatcacac
    10321 atctcaaagc tatacattga accacaatga aattggccgt gtacgaggtt tccaaaccga
    10381 agtggaagaa atggaacgtc aaaatgaaca gatgattcca cagatccgtc aacacgaaat
    10441 tccttattca gaagtgcgca cgttctataa aacagtcttc aaagtcttag aagatatcga
    10501 aaagcaacaa gtagagatcg acgactcatt acatgaattg agaaaaggcg aaaaagaagc
    10561 gcaagaaaag atcgatacat tcgaattcaa attgcgcagt ttgaaacgtt ttgtagaaaa
    10621 acaacgtttg cctggcttgc cgaatgatta cctagaattc ttttttgttg ctactgaccg
    10681 tattgaagaa ctaagtgttg tcttgaataa aattcgagta aacatggaag aggtcaatcg
    10741 tttagttgct ctatgtgaag aagatctaga attattagat aagaagacac atgatttagt
    10801 agatgcagca gcattaacgg agcaaatgct ccaatatgca aatcgttatc gtcatacaca
    10861 cgaagaagtg agagaagcaa ttgataaaag ctactattta ttcaataaag aatatcacta
    10921 tcaagaagca ttagacgaaa tcggtacagc attagagcga gtagaacctg gtgctttcaa
    10981 acgcatcgaa gatttttatt ttaatcatcc agacttagta taaagcaaaa aagttagtaa
    11041 accagcaaga gcaagacgga ttattgtctt gttcttgtct ttttttgtat atttgaaaat
    11101 tgaaacaagt gaaaatttca cctgctcttg aaagcacctt ggacaaccgt tataatagaa
    11161 aagaatttca aaaaagaggg gtttcttatg atatattttg ataacagtgc aacaacccca
    11221 atctttccgc aaagcttaga tgcatatgta aaaaccagtc agcggattat tgggaatcca
    11281 tcaagcttgc atgatttagg caatcaagct aaccggttgt tgcagcaagc aagaaagcag
    11341 atcgctgatt tgatccatgt agaaccagaa gaaatctatt ttacttcagg cggaacagaa
    11401 ggagataact gggtattaaa aggaacgatg atcgaaaaaa gagaatatgg gaatcatatg
    11461 atcatttcag gagtcgaaca tccggccgtc agcgagacag cagaacaatt aaaagaatta
    11521 ggaatcgaag taagtatcgc cccagtggat aaacgcggat ttgtacgtgt cgacgaattg
    11581 aaagaattaa taagaaaaga gacagtatta gtctctgtta tggctgtgaa taatgaagta
    11641 ggatcgattc agcccatcca ggaaatcagt gatcttttgg atgattttcc taaaattcat
    11701 ttccatgtag atgctgttca ggcgattgga aaagtaccat tctcgcaatg gttgacagaa
    11761 agagttgatt ttgcaacatt ttcagctcat aaattccatg gcccacgggg aactggtttc
    11821 atttattgga aaaagggtcg tcgtttagca ccattactga caggtggagg gcaagaaaga
    11881 aatcagcgaa gtgggacaga aaatgttcca ggtattgtgg ctatggttaa agctttacgt
    11941 ttatcattag agataaagga acaaaaacct gagcagacac aacgcttaaa agtagctcta
    12001 atcaatgcgt tgaagacgta tgagaaagtg acgatttttt cagaaggaac tgactatgca
    12061 ccgcatattt tatgttttgc tctaaaagga attcgcggcg aagttctcgt ccatgcctta
    12121 gaagaaaaac aaatatttgt ttctactact agtgcctgct cctcacgtaa acatatggca
    12181 agcagtacat tgcacgcaat gcatgtacca aatgatctgg ctacatctgc tgttcgaatc
    12241 agcttagatg aaaacaattc gcttgcagaa gtcgagcaat ttatgattat tttcaagcag
    12301 ctttataaga aattttctgc aatcaattaa aagaggagtg ttcttgtgaa atacagtgaa
    12361 attatggtcc gttatgggga actttcgaca aaaggaaaga atcgaaaaag ctttatcatg
    12421 caactagcac aaaatgtcag acaagctttg aaagactttc cagaaatcaa aatccatgct
    12481 gatcgggacc ggatgcatct tttattgaat ggcgcagacg ggcagctagt tattccaaaa
    12541 ctagcaaaaa tatttgggat ccaaaatttc tctccaagta ttcgtgtaga aaaagatgtc
    12601 gaagttttga aacaagctgt tcaagacatc atgaaagaaa tttatactgg gaaagaaaca
    12661 tttaaaatta tcgctaaaag aagcgatcac caatttgaat tgacaagtaa tgaattgaat
    12721 caaacattag gtaatgctgt ttttgatatt ttcccgcata tccaagttca aatgcgtcaa
    12781 ccagatattc cattacgtgt agagattcgg cgtgatggcg cttatctatc gtatgaaacg
    12841 atcaagggag ctggaggatt gcccgtagga acaagcggaa aaggaatgct tatgctttct
    12901 ggggggattg attctcctgt agcagggtac tttgccatga agcggggagt ggaaattgaa
    12961 gctgttcatt ttgctagtcc gccgtataca agtgaacaag cactgcaaaa agcaaaagat
    13021 ctaacagcca aactagcacc ttatgtggga acgatccatt ttatcgaagt gccatttacc
    13081 gagattcaag aagaaatcaa aaagtcagtg ccacaaggat attggatgac aattactcgt
    13141 cgaatgatgt tgcgtttgac cgatgaaatt cgccgtattc gtcatggttt agtcattttg
    13201 aatggtgaat cattaggtca agtggcttca caaactttgc aaagtatggt ggctatcaat
    13261 gaagtaacga atacgccaat cattcggcct gtagcgacaa tggataaact tgaaatcatt
    13321 gaagtagcag aacagatcga tacatttaat ctttctatcc agccttttga agattgctgt
    13381 acgatttttg ctcctccaca gccaaaaaca cgccctcaac tggaaaaggt ccatcaatat
    13441 gaagagcgtt tagcaataga tgaaatgatc caacgagcac tagctggctt gaagatggaa
    13501 gagatacaag cagaatcgtc taatgaaaaa actgaagaat tttcagaatt gctataagag
    13561 gttatgaaaa tagtgttttg accagagtat tagagaaaca aaacgaaaaa atagctccat
    13621 gtattttacc gtttttgaac taatgtcgaa gattagctga ttgaatacca tttatcaaga
    13681 agttatggaa aagccggttc ttttttacta actgaaaaat aaaattttat ttagagatca
    13741 agctacagtt gataaatgat ataaaaaaga gaatttccca tgaaatagcg aggttgtgac
    13801 agaagtgggc agcttcaaga aataagaagg aatttacgaa aattgctttt caaatttttg
    13861 tgaaatttcg gcttattttt cgaagaagtt acttctggaa caccgtgtat cagaggttgc
    13921 gaaaaagctg ccctgcatca agtaataaga acagccaaac aaaaatagtt cctcatattt
    13981 tacgtttggt tgagcttatt accgcagatg caagcttttg aacaccgttt tatttgtctc
    14041 acaccctttt ccttcctact tgtaaccttg ctcccgtgtt agaaacatgt tacactaaat
    14101 atgtgaaatt attaacaagg agcgtcgtta tgcgagaaaa aacagataca ctgccaaaag
    14161 caactgcgaa aagattacct ttatatttac gctacttgaa gatgctggat gactcaggta
    14221 taagtagaat caaatcaaat gagttcagcg aaatcaccca aattccttct gcaacgatcc
    14281 gcagagactt ctcgcaatta ggcgagctag gcagaagcgg atatggctat gatgtccctt
    14341 ttcttatcga tgtattcaat aatatcctta atactaaaga agaaaaacga atcgcattag
    14401 taggatatgg aaatcttggc aaagcattga aacacaacaa ttttagaaga aatgaaaatc
    14461 taaatatcgt gtgcgtcttt gataatgatc cagcactgat caatcgagta attgatggag
    14521 aaatgatcta tccgattgat cgtttcgcgg agatagccaa agcaaaaaat gtgacagtag
    14581 caatctctac ggtaccaagt aaatattcac aatctgctat tgatgaaatt gtcaaaggaa
    14641 atgtaaccgc tatacttaat tttgcgcctg acagagtaac tgtcccagct tatgtcaatg
    14701 ttcaatacat tgatttgaca accgagttgc agacgcttat ttactttgat gagaattact
    14761 cagaagtttt ttcttaggaa aatttgagta ggcttattga tcttgcgaga actcgtcgta
    14821 attctttcaa aaatttttta gtactaaatg aagtggtttt ttcacgacca cttgttgact
    14881 aaaaaaaaaa accattgtag aatgaagcat aacgctgaac aagaggagta actggcacat
    14941 tcattcttag agagagaact atctgctgaa aggttctcaa tgacaaccag tgaaggtagc
    15001 ttgggagttt ctgtcttgaa tggagtagag atagacgagt cgtgatcgtt aactcacttg
    15061 agaggcaata atgattgatt attgcaattt aggtggtacc gcgtaaatca tacgtcctag
    15121 aatacgaaag tattttagga cgttttttag tttaaaggag gaaaacagca tgacggaaga
    15181 aaataaccta tcaacaaaat ataatccgca agaagttgaa gctggacgtt atcagcattg
    15241 gttagaacaa gatttgttta aaccgagcgg agataaaaaa gcaaaacctt attcaatcgt
    15301 aatcccgccc ccaaatgtta ctggaaaact tcatttaggg catgcttggg atacgacctt
    15361 gcaagatatg ctgattcgtc aaaagagaat gcaaggatat gatactttat ggctgcctgg
    15421 tatggaccat gcaggaatcg cgacacaagc aaaagtagaa gcgaagctta gagagcaagg
    15481 gatttctcgt tatgatctag gtcgtgaaaa gttcatcgat caagtgtggg aatggaaaga
    15541 agagtatgcg agccacatcc gcgaacagtg ggctaaaatg ggcttatctt tagattacag
    15601 tagggaacgt tttacattag atgatggatt atctgaagca gtacgtaaag tttttgttac
    15661 cttatacgaa aaaggtttga tttatcgtgg tgaatatatc atcaactggg atcctcaagc
    15721 acgaacagct ctatctgata tcgaagttat tcataaagat atcgaaggcg ccttttatca
    15781 tatgagctat gaactagcag atggatcagg cgtagtagaa attgcgacaa cccgtccaga
    15841 aacgatgttg ggagatacgg cgattgctgt tcatccagaa gacgagcgtt atcaagcgct
    15901 catcggcaaa aaagtgattc tacctttagt caataaacaa atcccaatca ttgcagatga
    15961 atatgtagat atggaattcg ggacaggtgt agtaaaaatc actccagcac atgatccaaa
    16021 cgactttgag gtaggtaatc gtcatgatct tccacgcgta aatgttatgg atgatgatgg
    16081 tacgatgaat gaactagcag gaaaatatgc tggaatggaa cgttttgctg ctcgtaaagc
    16141 aatcgtgcaa gatctgaaag aaatcggtcg attgattaaa atcgatacga tgatccacag
    16201 tgtgggtcat tccgagcgga caggtgtcgt tgttgaacct cggctgtcta ctcaatggtt
    16261 tgtaaaaatg gcgccgttag cgaaaaaagc cattgataac caagaaacag atcaagcagt
    16321 ggaattcttc cctccaagat tcaatcaaac gtttttacgt tggatggaaa atgtccatga
    16381 ttgggtaatc tcacgacaat tatggtgggg acatcaaatt cctgcatggt atcacaaaga
    16441 aacaggtgaa atgtatgtag ggatggaagc accagcggat agtgaaaact gggtacaaga
    16501 tcctgatgtt ttagacactt ggtttagttc agctttgtgg cctttctcaa caatgggctg
    16561 gccagatacg gaaagcgaag attataagcg atacttccct actagcactt tagtaaccgg
    16621 ttatgacatc attttcttct gggtaagcag aatgattttc caaagcttgg aatttactgg
    16681 cgaacgtcca tttgagaatg tcttgattca tggattgatt cgtgacgaac aagggcggaa
    16741 gatgagtaaa tcattaggta atggaatcga tccgatggaa gtaatcgaaa agtatggagc
    16801 agatgctctc cgctggttct tatcgaatgg ttctgcacca ggacaagatg tccgcttcag
    16861 ctatgaaaaa atggatgctt cttggaactt tatcaacaaa atctggaatg cttcacgttt
    16921 cgttatcatg aatatcgaag gaatgaccgt agaagagatc gatttatctg gagaaaaatc
    16981 agtggctgac cggtggatcc ttactcgttt gaatgaaacg atcgaacgag taactgagct
    17041 atttgatcgt tttgaatttg gtgaagcagg tcgacaactg tataacttta tctgggatga
    17101 tttttgtgac tggtacatcg aaatgagtaa agaagtactt tatggagatg atgctgtttc
    17161 aaaacaaacc acccgtagca tcttagtcca tacacttgat cagatcctac gtttattgca
    17221 tccaatcatg ccattcgtta cagaagaaat atgggaacat attcctcatc agggaaattc
    17281 tctagtagtg gcagaatatc ctgttgtacg tccagagttt gatgatgaaa cagcttctaa
    17341 agggatggaa gtattgaaag aattgattcg agccgttcga aatattcgtt cagaagtgaa
    17401 tacaccattg tctaaaccaa ttacgttgct aattaaaaca aacgatgcta aaatcgatca
    17461 atttttagtt gaaaatacaa gctatatcga acgcttctgt aatcccgaag agttgacgat
    17521 atcaagtgag atcactgcgc cagatctagc gatgtcagct gttctgacag gtgcagaaat
    17581 cttcttgccg ttagcaggat tgatcaatat tgaagaagaa atcaaacgat tagaaaaaga
    17641 actggaaaag tggacaagcg aagtaaaacg tgtccaagga aaactttcta atgaacgttt
    17701 tgtttctaac gcgccggaag aagtagtaga ggcagagcgt gcaaaagaaa aagattattt
    17761 agagaagcaa acggctgtca aagaacgaat cgaaagtttg cgtactattc aataagaata
    17821 gaaaaagaga gtgtgaaatc agctagctat tactaaaagc tagctgattg aacatcgtta
    17881 caggaaagag tttgagcctc actcttagct tcgattcata aggagatgtg acataaattt
    17941 tgtcgcatct cttttttagt ctactcttat tatgtaggag aaggagggag gtggatcatg
    18001 tatcaagagt caataaagta gttgataaaa tcgttcctct ctctgtttct tttctctttg
    18061 tcatactttt atttttctta tgtgttctat ttgttataat aaaagagaaa tgggggaatt
    18121 gttgatgacg attgaagaag caatagaatg gatccatagt cgattaccat ttggttcacg
    18181 tcctggcctt gaccgagtcg aagctttatt agaagtggta gggcatccag agagaaaagt
    18241 aaaaacgatc catatcgctg gaacaaacgg aaaaggttct actgtagctt atttgagaag
    18301 cctattagaa gaggctgggc tgacagtagg tacatttact tcaccttata tcgaaacgtt
    18361 caatgaacgg atcgcaatca atggcaaaag cattccagac aatgatttga tccagcttgt
    18421 aaaaaaatat cagccactga tcaaacaact tgatcaattt gaagcagtag ctggtattac
    18481 tgaattcgag acattaacag caatggcatt tgattatttt ttgatgaaga aagtggacgt
    18541 agcgattatc gaagttggct taggcggctt attggattct accaatgtag ctgtaccaat
    18601 gctgacggga ataacaacga ttggacttga tcataccgaa atacttggag atacaattga
    18661 agaaatcgct gcacaaaaag ctggaatcat caaacaaggc gttccagtag taacaggaaa
    18721 tattgcccca gaagctttta ctgtcatcca acaaacagct aaagcaaaaa atgcaccgct
    18781 ttatgtatac ggggaaaatt ataaaataaa ctatctgcat cctgatgaga tgtggggaga
    18841 agtatttgaa ttttttaatg aaataggaaa gatccctcaa ttaaccactc cgcttttagg
    18901 gcaacatcaa gctgaaaatg caggagtagc catcgaattg tattttgttt actgtcaatt
    18961 ggaaaagctg cctttccgag aaaaagatgt tttgaaggga ttgaagaaat ctcgatggcc
    19021 aggaagaatg gagcgtttga gtaacgagcc attagttgtt ttagatggtg cccataatac
    19081 gcatgcaatg aaccggcttg ttgagaattt gaaaaaagaa ttcaaaggat atcaaatcaa
    19141 cattttgttt tccgccttac gaactaaaaa tgttagagaa atgctagaac gattgcagca
    19201 agtaccaaat gtccatattt atttgacgac ctttgaacat ccaaaagcca tcgaattaag
    19261 tgaagtatca gatttagcaa gtgagagagt atcaatcgtt tcattgtggc aattcggatt
    19321 agcagatata ttggaaaaga tgacaaatga agacgtatta gttgtgaccg gttcacttta
    19381 tttcatttca gaagttcgcc atttattaat acaattagga ggagatcatg aagtataatg
    19441 cagtgatttt tgacatggat ggtttgctgt tcgacacaga gttagtgtat tacgaagcca
    19501 cacaagtagt agcagacaag atgggttttc cttatgataa agacctgtat ttaaaattct
    19561 taggtgtttc agatgaagaa gtatgggcga attatcatat gattttctcg gaatttggca
    19621 aaacaagtgt acaaagattc atagatgatt cataccagga aacgcttgaa cgtttttcgc
    19681 aaggagcggt acaaatgaag cccggtgtgt tggaactcct ctctttttta gaagaacatc
    19741 agattcccaa agttgtcgct tccagtaacc aacgagcagt cattgaactt ttattgaaga
    19801 agaacaatct gtttgaacga tttgagacga ttgtttctgc agaaaatgtt caacgagcaa
    19861 aaccagaccc agaaattttc ttgttagcta atcagaagct aggtacagcg aagaaagaaa
    19921 cattgatttt agaagattca caaaatggta ttttagcagc ttctgcggct gagatcccgg
    19981 tagtcatggt accagatctg cttccaccat cccaagaatt gatcaaaaaa acagctgggg
    20041 ttgtttcttc tttacatgaa atccctgcat tgttaaaata aaaatccatc tcttttcgta
    20101 tcttatttta gataagatag aaaggagatg tttttttggg aaaacgattg atcaaagaag
    20161 tacctactag ctctttacca agggaacgta tggaaattta tggcgcggaa gctttatcgg
    20221 atcaggagct tttggcaatc ttactgcgta ccggtcaaca cccctatagt gtgatgtcga
    20281 ttgctggaaa tctgttaaaa acgtttggag gactggcctc tttacgacag gctactttgc
    20341 atgagttaga agagattcga gggataggac gcgtgaaagc aatcgaaatc aaagccttga
    20401 tcgaattagg tcgacgaatt cagacggatc atgaaagagc ggctccaccg gttcgtacca
    20461 gctacgaatt ggcccagcaa ttgattctgg aaatgaaaga tcacaaacaa gaacatttgg
    20521 tttgtattta tttggacacg aagaatcaag tgatcttaaa aaaaacagtg ttcatcggtt
    20581 cgctgaatca gtcagtcgct catcctagag aaattttcca ttatgctgta cgttattgtg
    20641 cagcaaggat cgttttagcg cataatcatc cttctggtaa tgttattcca tcgcagcagg
    20701 atatgaattt taccaaaagg atacaaaagt gtggagaaat gatggggatc actgtactgg
    20761 accacttgat catcggacgc aaacgatatt ttagtttgag agaagaagga atgatggaag
    20821 agaagtaagt tgctttcttg caaaaaaaat gaagcaagtg tacaattata ttgtaatttt
    20881 gtttgggaat agaagttgaa acatctgttg cacctctatc gtacacgagc aatgttacaa
    20941 tttaaattag tgcttttggc acgaggagga tattattatg gaaaacggaa cagtaaaatg
    21001 gttcaacgct gaaaaaggat ttggatttat ctcacgtgaa gatggaagcg acgtattcgt
    21061 tcacttctca gctatccaag gtgacggatt caaaacatta gaagaaggtc aagcagtgac
    21121 tttcgacgtt gaagaatcag accgcggccc acaagctgca aacgttgtaa aagcttaatt
    21181 ttatctgtaa atcaaattga tggtagacat caatttttac gagagaacaa agcgagtaac
    21241 aaaaatacct gccacaagag acaatttctc ttgtggcagg tatttttttg tatgaaaaag
    21301 taaataaatt taagaggcta tgaaagagcc accttgtatc aaggaataag aacaaacaaa
    21361 aatggcttct catgtttttc atttgttttc tcttatattc aggacaaggt gctgatttca
    21421 taacctctta tttattcttc atctggtgtc attaccattg gcaaaatcat tggatgtctt
    21481 tctgtacgtt cgaataagaa aggctgaagc gcgccagtca tcgcttcttt caatttatat
    21541 tctgaacagt caggtccatt caaacattct cttaaagcgt ggaataagat tcgctgagct
    21601 tcattgatca tttctcctga ttctcgcatg tagacaaatc cacgagagag aatatctgga
    21661 ccagccaaga tttctttttt cttgatatcc actgtcgcga cagctaatac aagaccttct
    21721 tctgaaagca ggtgacggtc acgtaaaacg acattaccaa tatcaccgat cccgctacca
    21781 tcgatataca catctccagc cacaaaatgc ccagcaggac gcgcagaatc gctagttaac
    21841 gctaaaacat cgccattttc catgatgaag cagttttctg caggaacacc tacatcttgt
    21901 gctagagaag catgtacttt caacatgcgg aattctccgt ggacaggaat aaaatatttt
    21961 ggcttcatca aacgaagcat taatttttgt tcttcttgtc caccgtgacc ggaagtatgg
    22021 atgttattga tttttccgtg aatcacttct gcaccagctt cagacagcaa attgatcaaa
    22081 tgattgacac ttgttgtatt accaggaata ggtgaacttg aaaagatcac tgtatcgtct
    22141 ggctggattt ggatttgacg gtgagtacca ttcgcgatgc ggctcaacgc ggccatcgct
    22201 tctccttgag aacctgtaca taggatcatc gtttcattgg caggcaagcg attgatttcg
    22261 tgtgcatcta caaatgtgcc gtctggaact ttgatatatc ctaaacgttg accgttgacg
    22321 atggcatttt ccatgcttcg tccgaaaaca gcgattttac gaccagttgc cacagcggca
    22381 tctgctgctt gctgcaaacg gaagatgtta gaagcaaagc tggcaaaaat gatacgtcct
    22441 tcgatttttg aaaagatctt caaaatagaa tgcccaatcg ttttttctga tttagtaaat
    22501 gttggaatct cagcatttgt actatcagat aaaaggcaca agactccttc ttccccgata
    22561 cgagccattt tatgcaagtt tgctggttcg ccaacaggag tgaaatcgaa tttgaagtct
    22621 ccagtcgcta cgatatttcc agaaggtgtt ttcaccacga tccctaatgc atctgggata
    22681 ctatgggtcg ttctgaaaaa gctgacagaa gtctttctaa aacggatgat cgtatcttca
    22741 ttgatctcat ggagctctgc atctcgaagc aagccatgct catctaattt gtttgtgatc
    22801 aacgctaatg cgagaggacc agcatagata ggaatatttg cttgtttcag caagtagggt
    22861 attccgccga tgtggtcttc gtgcccgtga gtaatcacca aggcttttac tttgtgtaag
    22921 ttttgtatga tgtagctgta gtcgggaatg acatagtcaa ttccaagtaa atcgtcttcc
    22981 gggaatttga tcccggcatc aatcaaaatg atttcatctt ggaattgtac cccatacgta
    23041 tttttcccga tttcacctaa tccaccaata gcaaagacgc ctgtctcgtt atttttgatg
    23101 gaaagtttca tattaaaact ccactaatga gaaatcagca ttttcttttt cgtactctag
    23161 atgacttcct tctagaagtt gaacgaattc aatattgtat ggggtatttt cttccacacg
    23221 ttggcgagcg tctacatcgc tttttgcctc aatgtaaaga gattttgttt cttcccgttt
    23281 agggttgcgt acttttgttt cttgataata aactttatag atcattgttt tgtctccttt
    23341 atctgcgtaa agcagtgatt tagctagttg atttttttag acaacacaaa acgaggggac
    23401 aagccacctg gtttttcata tgctttgact agaatactgg attcgagtca aagagacaag
    23461 cctcgttttt gcttatctgt tcggatattt tttcaaccgt accttctgta taaaaaatat
    23521 acaacaatta attgttattc tagcataaaa cagcaaataa gtatagaaat caactagatt
    23581 gaaagaaaaa atcaatgttt atcatcccgc tataatcata atgacttttt tatgagagaa
    23641 gtgttctatt aataggctcg ttgtgctatc atttttagta atgaaaatgt aacaaaagat
    23701 taaaaggaga tgagaaagtg aaactaatgt ggcattatac catgcgttat aagaaattct
    23761 tgtttttcaa tttcatatgc gtatttggtt ttattttgat tgaattagga cttcctacga
    23821 ttttagcacg tatgatcgat gtaggaatcc gcaatgatga ttttgattat gtgaagcaac
    23881 aagggatatt tatgatcgtt attactgtaa tcggtattat tttgaatatc ttgttaggtt
    23941 attttggttc gaggattacg actaatatcg tagcagatat tcgagatgat ttgttcgaaa
    24001 agatccaaag ttattcgcat caggaatacg aaaatctagg cgtatcttca ttgatcactc
    24061 gtacaacaaa cgatgcttat cagatcatgt tgttcctgca aaatatttta cgtatcggtt
    24121 ttatggcacc aatgatgttc attgtcagtt tgtacatggt catgcgtaca agtgtgacac
    24181 tcagtttgta tgtagtaggt gcattacctt tactattatt ggcagtcgta gcgattgcta
    24241 aggtgtctga accgttatct aaaaagcaac aaaaaaactt agataaaatc aacagtatct
    24301 tgcgggaaaa tctttcagga ttgcgtgtga ttcgtgcatt cgtcaatgaa aagtttgaag
    24361 aaaaacgttt tgctgtagtc aatgagaatt atgcttctag ttcaaaaagc ttattccgtt
    24421 tgatggctgt tgcacaaccg ggtttttatt tcttatttaa tatcgtaatg gtattgatca
    24481 tctggagcgg ggcagtgcaa atcgatgcag gcgagcttcg tgtaggagat ttgattgcgt
    24541 ttatcgagta tatattccat gccttgttct cattcatgtt atttgctagt gtgttcatga
    24601 tgtatccaag agcagctgtt tcagctggac gtatccaaga agccctagat gccaaacctt
    24661 cgattgtaga agatccaaat ggtatatctg atacaaaaac taaagggtat atcgaatttc
    24721 aaaatgtaac atttgcttat ccaggccatt cgcaagagcc agtgatacga aatgtcagtt
    24781 ttacagcctc tccaggagag accgttgctt ttattggaag taccggtagc ggaaaatcta
    24841 cgttgatcca attgatccct cgcttttatg atgtgagtga aggaaagatc ttgatcgatg
    24901 gagtagacgt gagagattat cggctaagca agttgcgaga taagattggg tttattccgc
    24961 aaaaagcgct gctatttact ggaacgattg cagataattt gcgatacggg aaagaagatg
    25021 ctacgcaaga agaaatggaa cgtgcagctg agattgctca agcttcagaa ttcatttctc
    25081 gaaaaccaga tggatttaat gaacatttat cagaaggtgg ttccaatttt tcaggtggac
    25141 aaaaacaacg attggcaata gccagagcca tcatacgccg tccggaaatc tatatttttg
    25201 atgacagctt ttctgcatta gattatcaaa cggatgcgaa gttacgtgct cgtctgaaaa
    25261 aagaaacgac agaatctact gtattgatcg tagcacaacg tgtgggaacg atcatgcatg
    25321 cagataagat catcgtatta aatgaaggag aagtcgtcgg tatggggacg catcgggagt
    25381 tgttggaaaa atgcccgatt tattatgata ttgccgcttc tcaattatca aaggaggaat
    25441 tagcatgaaa aaaacctttt cttccttaaa acgtttagct cgatatatca gaccgtatcg
    25501 tgagactttt cttttagtga tcctatttac agttctgaca gtggctttca acgtggcttt
    25561 gccatatgtt acaggacttc ctactacaga aatcagccgt aatattgcca atggcgaatc
    25621 aatcaatttt tcttatatat tcaaatgtct catttggatc acagttgttg gaatagggta
    25681 ttgtatttcg caattacttt ctggtgtttt gatgacaaat gtcgttcaaa gtgccatgca
    25741 tgatttgaga agagacatag aagaaaaaat caatcgtctt ccggtttcat atttcgataa
    25801 aaaccagcaa ggaaatattt tgtctcgtgt gacgaatgat gtagatgcag tcagcggtgc
    25861 tttacaacaa gcttttattg gtatcgtaaa tgcgatattg ggaattacgt tggctgcagc
    25921 aatgatgttt tatatccagc cgatgatggc gctgatctct atgatcatga ttccaacatc
    25981 gatctggatc tcaaaacgag tgatcaatgc ttcgcagaaa tattttcaaa gcatgcagaa
    26041 ttctcttggt gagctaaacg gctacgtcca agaaaatatg acaggtttta gtgtattgaa
    26101 agtatatgga cgcgaaaaag aaacattgga aggattcaaa aaagttaatc acagcttgaa
    26161 atactttggg tttcgtgcag cattcatttc tggcttgatg atgcctctag ttcaattaac
    26221 tgcctatgct acttatattg gtatggctgt tctaggaagc ttttatgtta ttactggggc
    26281 gatcgttgtc ggtcaactac aagcattcat ccaatatatt tggcaaatca gtcagccaat
    26341 ggggaacgtg acacaattgt cttcagtttt gcaaagtgca tcagcggcaa cgaaacgtgt
    26401 attcgagatt ttagatgagc cagaagaaaa agaaaacaaa gtagatgctt cactgccaga
    26461 aacgatttta ggagatgtga cattcgatca tgtcgatttt gcatatgatc cgaagaaacc
    26521 attaatccaa gatttgaatt ttgaagtgaa agctggtcaa accgtagctg tggtcggtcc
    26581 gaccggtgcc ggcaagacga cattgatcaa tctattgatg cgtttttatg acgtagacaa
    26641 aggagcaata aggattgatg gaatcgacac caaatcaatg agtcgcagtg atgtgcgttc
    26701 gctatttggt atggtcttgc aagatgcatg gctttatcaa ggaacgattg cagataatat
    26761 ccgcttcgga aagcttgatg cgacagatta tgaagtagtt gatgctgcga aaacagcaaa
    26821 cgtagatcat tttatccgaa cgatgccaga tggatatgat atggccatca attctgaggg
    26881 agacaatgtg tctttaggac aaaaacagtt gctgacgatt gcccgagcag tggtctccga
    26941 tcctaaaatc ttgattttag atgaagcaac aagttcagta gacacccgtt tagaagcgtt
    27001 gattcaaaaa gcaatggatc gcgtgatgga gggacggaca agtttcgtca tcgctcaccg
    27061 tttatcgaca atccgtgatg cagatctgat tctagtgatg gatcaaggtc agatcattga
    27121 aaaaggaacg catgagagtt tattggcaca aggtggattt tatgaaaaac tgtataacag
    27181 ccaatttgct gaggaagcag aataagtcat ataagaataa aacagtttaa aacttatttt
    27241 ttgttctcta ttcttgataa tggtaaagta aagttatcaa gaaaagtgag gacacggaga
    27301 tggaacagtt tttaaatgaa aaaattactg aagccaaaag agaagttgat cgtttaacgc
    27361 acgtctgtca gacgaagcct tttgattcag ctgatttttt tgaaaatgag tttcaaatcg
    27421 aacgcttggc agcacaaata gaagtgtacg aattaatttt aaaaaaagta atagaagaaa
    27481 atatagatgt acaataaaaa caggtaaaac gtcagttacc agatactgta aatagacatc
    27541 tggcctgagc aggttttacc tgtttttttt cttatgaaat aaatatatgc tatgatacct
    27601 ttattcaata ggattagttt ctacaaaaaa tgaaaggaga aaaataaata tggaacggtt
    27661 tatagcatca aatgccacag tgattggtga tgtgacattg tcagaagatg tgacgatctg
    27721 gtatcaagct gttttacgcg gggacagtaa ttggataaaa ataggacaaa gaacgaatat
    27781 ccaagatggt acaatcatcc atgtcgatca tgatgcacct gtagatattg cggagaatgt
    27841 gacggtagga catcagtgca tgctacatgg ctgtacaatc gaaaaagggg cgctgatcgg
    27901 gatggggacc acgatattga atcatgcggt tattggtgag aatagtttga ttggtgctgg
    27961 atcattagta acagaaggaa aagttattcc tcctaatgtc ttggcttttg gtcgcccagc
    28021 taaagtgatc cgtcctttga caaaagaaga aatacaaaaa aacaaagaaa acatccagca
    28081 ttatgtcgag ataggtcgga tgcatgcgca aggaaaattc aaggagcaaa gataggcagt
    28141 agcatccata aaataaaaaa ggatggtcag tttgagttga ccatcctttt ttatttggtt
    28201 tagataagag agcttaaaaa agcttccttt gaaacagatt aatttaatga agaactgttt
    28261 tccatgactt catcgatcaa tccatatgct tttgcttgtt cagctgtcat atagttgtca
    28321 cgatctgtat ctttttcaat cacttcaagc ggttgtccag tgcgttcagc taaaattttg
    28381 tttaagcgtt cgcgtgtttg caaaatatgg cgtgccgcaa tttcgatttc agttgcttgc
    28441 ccttgagcgc cgccaagagg ttgatggatc atgatctcag catttggtaa agcaaatcgt
    28501 ttaccttttg taccagctgt cagcaagaag cttcccattg aagcagccat acccataaca
    28561 attgtttgga catctgcttt gacaaagttc attgtgtcgt aaatagccat acctgctgta
    28621 actgaaccac caggagaatt gatataaaga tagatatctt tttcagaatc ttgagcgtct
    28681 aagaaaagca attgggcgat gatcgaatta gcaagatcat ctgttacttg gccgcttaac
    28741 atgatgatac ggtctttcaa taaacgtgag tagatatcgt aagctctttc acctcgtgaa
    28801 gattgttcaa tgactgtagg aattaaattc ataatagagt tcctccttga tttcgtttag
    28861 aaatagtgta ccataattta gtttctaata cctaaaatgt agtatacatt tttggtcaaa
    28921 aaaggtcaaa taaaaaactt aggcaaatta gtcgcataaa cccgctttat ggtatgataa
    28981 gcagagaaac aaaacaagga ggaacaacag attgatagtt ggtattactg gccttatcgg
    29041 agtgctgaca ggtgctgtag ttgtctatat cggcatgata ctgcaaatgc gtcacttgga
    29101 aaataaagaa cagcgtagac gtgaattgga attgaaagtc aaagaaatcg agacgctgaa
    29161 tcaattaaat aaaaagatca acgagatctt gcaaaaacgg acaacgctga tggaagaata
    29221 tgtcagcttt gatgcatttg atgattgtta tatcacgatt gacgattttg cgtatctcca
    29281 atcattttca gcgcaaaata gtttttatct gccaaattat attttagacg agctatttaa
    29341 gaatatctcc catcgaaaag tgattttatc tccagatgaa acaatgaaga ttggtggata
    29401 tgcttataaa ggcggacgcg tgattttaga gaattttacg gatagtctga ctgaaatgat
    29461 caatgaaaaa aaagctcagt tgaagactat gacgcgccag ccgattggtt tcttctcgaa
    29521 agatgatgtt tggagctgat attttgaagc attgccgttg cacatgagag cgtggtaatg
    29581 ctttcttttt ttcttaatga tacaatagaa ctgaacggag tgaagaaaga tgttacaaat
    29641 ctatgcccat cgcggaagca gcggcaccca cccagaaaat acgcttcctg catttgcaga
    29701 agctgtacga gtaggtgcag atggtatcga actagatgtc catctaagca aagatggtta
    29761 tttgatcgtt atgcacgacg aagaagtgga ccggacgact aacggtaaag ggctgattcg
    29821 agaaaaaaca ttggaagaac tcaaaaaatt gaatgcaggg ggcaaattca gcaaagaatt
    29881 tccagcagct aaagttcctg aattgagaga agtgttagga ctgctcctcc aaaaaaatta
    29941 ccgagggatg ctaatgattg aaattaaaac tgatcagtat gaatatcccg gtatcgaaga
    30001 aaaattagcc gaaacattaa aaagtagaga ttggcctttt caacatgcat attgcagctt
    30061 tcatttggat tctttggaaa aaatcgcatt actggaaccg gagatacagc tggacttatt
    30121 gatgagtact tctgataaaa agatccaact agcagcagaa accccttata tagaaggagt
    30181 acatccaaaa aatacttggt tgttcgaaca tccagtccag gcagctgatt ttccaaaagc
    30241 aattcgtgca tggacgatta acgatgaaga agaaatggcc aaatgtcaag aactcaaaat
    30301 agatggaata ataactgatt ttccggagaa agctcttcgg tggaaacaat caacgaaaag
    30361 taggtaaaat atggaaaaag tattagtgat cgtcggtccg acagcagtcg gtaaaacagc
    30421 gctaagtgtg gaactcgcac aactatttgg aggagagatc atcagtggtg attcattgca
    30481 aatctataaa aatctggata tcggtacagc aaaaatcaaa gaagaagaga tgaataatgt
    30541 tccgcatcat ttgattgatg tgatcgaacc tactcaacgt tactcagccg ctgattttca
    30601 gcattcaggt cgacaattga ttaccgagat tgcgaaccga gggcacctac ctatcatagc
    30661 tggtggtacg ggtctctata tccaatccct tctatatgat tatcagttag gagcgacaga
    30721 agaagcgaat cctgagatta gaaaaaagta tgaaacgtta gctgaaacaa ttggttccca
    30781 agcattatgg aatcagttga aagaaaaaga cccggcagct gctgagaaaa tccactggaa
    30841 caatcaacgc aaagtgataa gagctctgga agttttagag acgacaggac atagtatcac
    30901 ggcacctaaa gaagagccaa agcaattgta tgattacctc atgattggat tagatacgaa
    30961 gcgtagtatt ctttatgaac gaatcaatca aagagtcgat ttgatgctag cagaaggttt
    31021 agtagcagaa gcgcgtaagg tgtatgagct tggagaagtc caggccagtc aaggtattgg
    31081 atataaagaa tttttccctt attttgaagg gaaagaaaca ttggaagaag caacagaaca
    31141 agtcaaacta cattcgagaa gatacgcgaa acgtcagttg acgtggtttc gtaaccgttt
    31201 ggatgcacag tggtttgatc ttataagtga gccaaatcaa aaaagtcaga ttgagcaaac
    31261 aatcaaaaaa tggctggagg cggaataaaa tggaagaaaa agtgatcatc gtaggggtag
    31321 aaaccgaagc aaaccagcgt tacttttcag agtcaatgga agagctagtt cagctgacga
    31381 ataccgcatc aggagaagtc gtattcacaa ttacacaaaa gagaccacaa gtggatcggc
    31441 aaacgattat cggtaaaggg aaattacaag aattagttca acaagcggat gcgcatgaag
    31501 cggatttgat catctttaat catgagctaa ctccgagaca gagccaactg attaccgatg
    31561 ctgttggtct tccggtaatt gatcgcgtgc agcttatctt agatattttt gcgatgcgtg
    31621 ctcgttcgaa agaagggaag ctgcaggttg aacttgctca gttagaatac ctgctgcctc
    31681 gtttagtagg gcaagggaaa acattatctc gtcttggcgg tgggatcgga acaagagggc
    31741 caggtgaaac aaagttagaa acagaccgcc gacatatacg gaataaaatt tttgctgtta
    31801 agaaagaatt gaaagaagta gaagctcatc gagagcgcaa tcgccaaaaa agaaaaaata
    31861 gcgagatttt tcagattgga cttattggct acacgaatgc cgggaagtca actatcttga
    31921 atctattgac acaagcggat acttattcga aagatcaatt gtttgcaacg ttagatccat
    31981 tgactaagag atggcgtttt gctgaaggat ttgagattac tgtaacggat accgtcgggt
    32041 tcatccaaga cctgcctacg caactgatcg atgctttcca ttctacatta gaagaaagtc
    32101 aaaatatgga tcttttgcta catgtagtcg atgctagttc tccagatcgt attttacagg
    32161 agcagactgt cttaaaattg atggatgaac tgaatatgaa agaaatgccg atactgactg
    32221 tttacaataa ggcagaccag atcgacccag cgatgttcac gcctacgcta ttcccaaatg
    32281 tattgatttc tactcagacg aaagaaggta agatggcgtt gatcgaagca atcaaacgtc
    32341 agctgatgga actaatgacc ccttatacac tttctgtgcc aagcaacgaa ggacagaaat
    32401 taagcgagtt gagaagacaa acaatggttt tggacgaagc gtacatggaa gaaacaaacg
    32461 aatatgaagt taaagggttt gcaaaaacca attcaaaatg gttaagaaaa aaccaataaa
    32521 attttatttt tttaa
//
LOCUS       DACDPS010000024        31719 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.024, whole genome shotgun sequence.
ACCESSION   DACDPS010000024 DACDPS010000000
VERSION     DACDPS010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.024"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..383)
                     /locus_tag="IWJ07_001188"
     CDS             complement(<1..383)
                     /locus_tag="IWJ07_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9332779.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKK"
     gene            658..870
                     /locus_tag="IWJ07_001189"
     CDS             658..870
                     /locus_tag="IWJ07_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9332780.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            873..1085
                     /locus_tag="IWJ07_001190"
     CDS             873..1085
                     /locus_tag="IWJ07_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332781.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            complement(1377..1601)
                     /locus_tag="IWJ07_001191"
     CDS             complement(1377..1601)
                     /locus_tag="IWJ07_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004401476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP9332782.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            1679..1867
                     /locus_tag="IWJ07_001192"
     CDS             1679..1867
                     /locus_tag="IWJ07_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332783.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            2193..2528
                     /locus_tag="IWJ07_001193"
     CDS             2193..2528
                     /locus_tag="IWJ07_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332784.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            2761..3702
                     /locus_tag="IWJ07_001194"
     CDS             2761..3702
                     /locus_tag="IWJ07_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAP9332785.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            3704..4594
                     /locus_tag="IWJ07_001195"
     CDS             3704..4594
                     /locus_tag="IWJ07_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HAP9332786.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            4637..5437
                     /locus_tag="IWJ07_001196"
     CDS             4637..5437
                     /locus_tag="IWJ07_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP9332787.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADDIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            5452..6303
                     /locus_tag="IWJ07_001197"
     CDS             5452..6303
                     /locus_tag="IWJ07_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9332788.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            6342..6503
                     /locus_tag="IWJ07_001198"
     CDS             6342..6503
                     /locus_tag="IWJ07_001198"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332789.1"
                     /translation="MLQIIGDRDMDELSQQESRMVRKISKLIIYRDTERFLTVLGNVY
                     ENPELLEVE"
     gene            6504..6965
                     /locus_tag="IWJ07_001199"
     CDS             6504..6965
                     /locus_tag="IWJ07_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010781228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9332790.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            6991..7443
                     /locus_tag="IWJ07_001200"
     CDS             6991..7443
                     /locus_tag="IWJ07_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332791.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            7549..7743
                     /locus_tag="IWJ07_001201"
     CDS             7549..7743
                     /locus_tag="IWJ07_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332792.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNEFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            7774..8067
                     /locus_tag="IWJ07_001202"
     CDS             7774..8067
                     /locus_tag="IWJ07_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332793.1"
                     /translation="MKLKDGFYSSGHGIGGLMLDMPTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVVRLVDMEVIKS"
     gene            8097..8537
                     /locus_tag="IWJ07_001203"
     CDS             8097..8537
                     /locus_tag="IWJ07_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9332794.1"
                     /translation="MSVIPQLEGEEAIVNILENIDIKQTRKNARRLLKRYRNLERLVG
                     PVKIDFSVMTVTKNLKFTIDSQNEEIIEAISTRDSVIEALTRLSRIHFQVLYYSYCFP
                     NKMSMYQIGEKLGYSDRTIERMKAVALVEFAEAYKSGELISRTK"
     gene            8612..9079
                     /locus_tag="IWJ07_001204"
     CDS             8612..9079
                     /locus_tag="IWJ07_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HAP9332795.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQHR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            9826..10056
                     /locus_tag="IWJ07_001205"
     CDS             9826..10056
                     /locus_tag="IWJ07_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332796.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMGREAE
                     PCEIDACKLVYCGHGFYDEPNIQNNINKNLRD"
     gene            10215..11048
                     /locus_tag="IWJ07_001206"
     CDS             10215..11048
                     /locus_tag="IWJ07_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small subunit of terminase"
                     /protein_id="HAP9332797.1"
                     /translation="MARKRDPRRDEAKKIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELKSNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPKRNKNALKTGEYET
                     IFFDTLSDDEKDIYSSLDDDPSFVLSEEIRLLKIRQLRMMKRIKEAESGLNDEEVDRL
                     QQMRKIKTPIEKDGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            11041..12456
                     /locus_tag="IWJ07_001207"
     CDS             11041..12456
                     /locus_tag="IWJ07_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase B"
                     /protein_id="HAP9332798.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPENMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTTVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            12468..13997
                     /locus_tag="IWJ07_001208"
     CDS             12468..13997
                     /locus_tag="IWJ07_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP9332799.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKAPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            14089..14967
                     /locus_tag="IWJ07_001209"
     CDS             14089..14967
                     /locus_tag="IWJ07_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAP9332800.1"
                     /translation="MMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            14969..15286
                     /locus_tag="IWJ07_001210"
     CDS             14969..15286
                     /locus_tag="IWJ07_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332801.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            15335..16036
                     /locus_tag="IWJ07_001211"
     CDS             15335..16036
                     /locus_tag="IWJ07_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HAP9332802.1"
                     /translation="MVEWWGATIKTQSNAGRENESWGERRMSMKHKSLMPMNLQYFAE
                     GDDQKFSFDDFKSFVESNEEAQKFIQSQSQSVADKQLEAWKQNNLDKIKQDTIKEYEE
                     SKKNKSPEQIQLEKLQAEFEAEKALRVTSDNKAFVAEQIAGLELDGELKESISQFMLN
                     NLVSSDTDFTKNAVEGFTSVLNAINEKHADALKELQMKSAFGGTQQSNNQVQQNNETL
                     TNPEEQLGQILQQFN"
     gene            16051..16941
                     /locus_tag="IWJ07_001212"
     CDS             16051..16941
                     /locus_tag="IWJ07_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HAP9332803.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENSSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            16965..17180
                     /locus_tag="IWJ07_001213"
     CDS             16965..17180
                     /locus_tag="IWJ07_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332804.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            17192..17533
                     /locus_tag="IWJ07_001214"
     CDS             17192..17533
                     /locus_tag="IWJ07_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332805.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            17533..17904
                     /locus_tag="IWJ07_001215"
     CDS             17533..17904
                     /locus_tag="IWJ07_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332806.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            17897..18292
                     /locus_tag="IWJ07_001216"
     CDS             17897..18292
                     /locus_tag="IWJ07_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAP9332807.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            18294..18671
                     /locus_tag="IWJ07_001217"
     CDS             18294..18671
                     /locus_tag="IWJ07_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332808.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNNPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            18672..19280
                     /locus_tag="IWJ07_001218"
     CDS             18672..19280
                     /locus_tag="IWJ07_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP9332809.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            19280..19621
                     /locus_tag="IWJ07_001219"
     CDS             19280..19621
                     /locus_tag="IWJ07_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332810.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            19863..22652
                     /locus_tag="IWJ07_001220"
     CDS             19863..22652
                     /locus_tag="IWJ07_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HAP9332811.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANPFVLIIGAVVGAI
                     AVFGYFMATNEELRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNGIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNIYFAALNI
                     WTGFKLTLINIWNEVVNQAKSIWLNIKYFFINLWIDIKYFAIQKWIELKFSIIQTWID
                     LKYNAITTWNNIKQFFKDTWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVN
                     SAENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTI
                     KGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTP
                     SVQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQ
                     MKRDAGAVGGTLYAT"
     gene            22642..23382
                     /locus_tag="IWJ07_001221"
     CDS             22642..23382
                     /locus_tag="IWJ07_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP9332812.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYASKP
                     LIKLSGSGDASFYINNDKYDLKLLDRELYIDSKLEESYRKLDDNLEHQDHVTLFLDFP
                     FLYPGKNEIKWTNNIHSFEIIPRWWRKV"
     gene            23379..26141
                     /locus_tag="IWJ07_001222"
     CDS             23379..26141
                     /locus_tag="IWJ07_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP9332813.1"
                     /translation="MIPRIYSPTETDFSTNGLGILKDTTKCEIYEVANGKYELELEYP
                     LGTRFDEYFENDYQIKAKSNDQEEYHIFFIDDKDIDTFLDTVTIYAQSRTNRLGRRAV
                     TFAEVDSKTGREAMAIIENNMDKKSDIRLYSDITTVSSTTFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRDNIGTIRYGKDLSGLKVKLDWTGVKTRIIPYADPQS
                     DAGTTSRIYGSPVDSEYINNYPDVYTEHVQFTEEQGVKDVNSLNKIAKNYFKTINPGC
                     DKPKISITVEFDKLTDTEEGKEFAKIRNYGLFDTFKIYHRKYKLYFESKVSGVQYDSL
                     SEKVLKLEAGDAQVAFYQQQAVTIQDKLKDYATNNYMSSFNDYVSSMIAGQGNAGGYV
                     VLWPKEKPSNIFIMDSPDLNKAKEVLRMNKNGIAFSKNGWNGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSNSSIGTI
                     GTTDSAGNPFPGASTPTPLEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWRIYDILCNNGFTK
                     QSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLF
                     NAAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGFKACTDPRQAAYAFERNYE
                     RPAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYS
                     GYLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIIN
                     WARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAISS
                     IVIPPK"
     gene            26154..27062
                     /locus_tag="IWJ07_001223"
     CDS             26154..27062
                     /locus_tag="IWJ07_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HAP9332814.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETRIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            27062..27679
                     /locus_tag="IWJ07_001224"
     CDS             27062..27679
                     /locus_tag="IWJ07_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332815.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            27683..28132
                     /locus_tag="IWJ07_001225"
     CDS             27683..28132
                     /locus_tag="IWJ07_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332816.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            28132..28626
                     /locus_tag="IWJ07_001226"
     CDS             28132..28626
                     /locus_tag="IWJ07_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332817.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVI
                     TYSI"
     gene            28640..29071
                     /locus_tag="IWJ07_001227"
     CDS             28640..29071
                     /locus_tag="IWJ07_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332818.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            29073..29210
                     /locus_tag="IWJ07_001228"
     CDS             29073..29210
                     /locus_tag="IWJ07_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP9332819.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITDEEADKIINKDKSA
                     S"
     gene            29247..29540
                     /locus_tag="IWJ07_001229"
     CDS             29247..29540
                     /locus_tag="IWJ07_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332820.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            29537..29761
                     /locus_tag="IWJ07_001230"
     CDS             29537..29761
                     /locus_tag="IWJ07_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP9332821.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            29758..30777
                     /locus_tag="IWJ07_001231"
     CDS             29758..30777
                     /locus_tag="IWJ07_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9332822.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            30967..31040
                     /locus_tag="IWJ07_001232"
     tRNA            30967..31040
                     /locus_tag="IWJ07_001232"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31001..31003,aa:Ile,seq:cat)
BASE COUNT        11392 a         4626 c         6557 g         9144 t
ORIGIN      
        1 tttttctttg ctttaacttt ggttaaggca agtgcaagtt gaatggcatc gtataatttt
       61 tccttatctt catcgtctaa aggttcacca taataatttg tttcagcatc tgaattcaag
      121 ccttcaagta atttttcagc ttgtaaagca atatcttgtt tatctttttc tgttaaatca
      181 taataatggc gtttatcagt acgtcctaaa agataatcag ttgatacatt aaagtaatct
      241 gcaacttttt gtattttatc tccagatgga gtacttttat tccatctaga catgcttcca
      301 tttccgaaat ttaactttct ttcaagttct gccaacgtca ttgaacgttc ttttaataag
      361 atttttatac ggtctaatag cataggatat gcattccttt caaaataccg acaaaaaaag
      421 tatttttctg caaattatct atttacaaat agagaatttg cagttatact agttttgtaa
      481 gcaagatatt tacggcatac aaagactaaa agcaataaac taatagtctc cccagacatc
      541 agtaagctcg ctttttgtgc gctgtatttt gtatgcttac ataatagcat atttgcagtt
      601 tttgtcaaac ttattagaca aaaatattag aaaatattcg ataagggggt atgaagcatg
      661 actttaaaac agcaagtatt aattgcttta gtaaagaaag gttggagtca aagagagtta
      721 gctcgacgaa tgggtatatc aattacttat ttacgagata tcttcatcga aaaaagaaaa
      781 cctaaggaac gtttaaaaca aatcgaagaa cttttggata tcaagttaga agtagattct
      841 aatgatcagc catcagaaca ggaggcgtaa aaatggtaca acgtttgagc aaagcggagt
      901 ttaaagagaa atataagtat tctgaaagta cttatcaaag aagaatgaag gaatttaaaa
      961 aatcacgttt tagtgagggg tataacgcag ttacttccaa agaaataaca attgatgtcg
     1021 atttatatga acagtttaga acatggcgtt ctaaaaatcg atttagagtt agagctgttt
     1081 gttaatagct aaaaaatcta aattcaccac ttctgaaaag aggagttgct gctctccttt
     1141 caagtttgcc aaagtccacg ttttttcatt ttggcaactc ctcttcttgg aggtggtgac
     1201 ggcgagagac gaaggaggga tgacatgaag gaagaatcaa aaatagggat tttgaatgag
     1261 aacaattaca caagattaat tattgaaaca gaagaaggga aaaaagtagc agaagttaca
     1321 ttgaccgatg caactcctgc tgacggttat cgtattcgat taactcctaa atatgattaa
     1381 ccttttggtg ggtgtggatc gtttccgtga ctatcacggc tttgaatttt tccatctctg
     1441 ccatgaataa atagctcaga tccttgattt ttagaaatct ctctagcgat ttttactgct
     1501 tctgattttg tattagtatg tgcagtcgct ttagaatttc cagcaccttt gacattccaa
     1561 ccaccattgg aattaggtac aacatgttga ttttttccca tagttttcac ctccatttct
     1621 atagaaatat tttatcaaat agaaatggag aaattcaata aacgaaagga gaagtgatat
     1681 ggcatatacc ttgcaacaag aacatcaaat tctcggtttg attaaacaac gcaggaaaca
     1741 attacaagat gaccgtgcag cgcttagaaa agccgatgag ctatcagata ggcaagctga
     1801 actaattgct tctgaacttg aggatttgag aatgctagaa ataaaaaata gggagattag
     1861 attatgaaga agacagacac actttttata ggattcattt tggggttatt agtgattgta
     1921 gcgcaccaaa gtattatcgg gggaagcttg ttcgcagcat tgatggtttt aatcaatctg
     1981 cttgattcaa aagaaaggag caactatggc acgagaagaa gcgctaaaaa tcggtaaagt
     2041 gattgctgat aatcggtggg gaaatagccg ccctattatt ttaagcaagc aacatatcga
     2101 aaagcaaaaa gcatggcaac aaataaaaaa gtgactctgc cagccagcaa agagtcacat
     2161 acaaatttat aaaactagga gaattatatc acatgaataa gaaaattgaa aatcttatcg
     2221 aagaattgaa acgtgaatgc caaaagcaag gtgtttctat tatttgtaca gcccaaaaag
     2281 aaggggaact taaaagtctc gtttatggtg agacaacaga aattttactt tgtcttgcta
     2341 tgcaagaaga acatttagat gaaaatctcc cattgtcagc acatattatg cgtagaattg
     2401 ctgtggatgc ttacgaacaa gcgaaaaatg aagaagaaaa tcaaccttca aatcatactt
     2461 ttgttattaa taacaaggaa gatttagctg atgtgatgac tcgtattctc aagggggaat
     2521 ttcaatgatg aatgaaaaaa caattgatct actcattatt ttatttttca cgttagtgat
     2581 tgtagcgaaa agttatgtaa tctttggctt gttattcgca attaccatgc ttgtgctttg
     2641 cgtgttagca agcagaaaag aggttaaaca tgaacgagtt agcaaatctt gacaactatt
     2701 taactgatcc tgattatgct aaaccaccat atgaagcacc aattgatgag gaggatgaag
     2761 atgagtaaat ctaccttaga aatgagccat caagaatggc ttgaagaccg taagaaaggc
     2821 atcggaggtt cggatgttgg aacaatttta ggattgaata aatggaaatc tccttatcaa
     2881 ctatggctag aaaaaacagg acaagtagta cttgaagaat caggaagtga gcccgcttat
     2941 tggggtaatg ttttagaaga agtggttgct aaagagtttc aggaacgtac aggcaaaaaa
     3001 gtacgcagaa gaaaccaagt atttgaacat ccattacatc catttttaag agcaaatatt
     3061 gatcgggaag tagtgggaga aaatgccatt ctggaatgca aaacagccaa tcaatttctc
     3121 ggtaaagaat gggaaggcga agaggtaccg cttagctatc tctgtcaagt tcaacattac
     3181 atgaacgttc taaacaaaga ctattgttac atcgctgtct taatcggtgg tcaaaaattt
     3241 atctggaagc ggattgaacg agatcaagag ctgatcgata caatcactga acaattagta
     3301 gagttttggg aaacaaacgt tcttggaggt atcgagccaa ttattgatgg tagtcaagcg
     3361 acagctgatt tcttgaaaga gaagtatgca gatgttgaag acgttcaaac gacgttacca
     3421 attcatttcg atgaactagt tgaacagaaa aacgaactca aacggactaa gaaagaaatt
     3481 gaatcagcta tccgtcaagt agacaacgag attatcagcg agctaggtaa acgtgaagcc
     3541 agtatcggta tcactcaaaa aaacatcatc agttggaaat ttgtccgtac gagacgtatg
     3601 aactcgaaga aactagcaga gaaatatcca gatgtcgcaa atgatgaaga gatttataac
     3661 gttactgaat caagaaggct aaccgaaagg gagatcaaat aatatggcaa caaatgaatc
     3721 gttaaaaaat caattggcag caaagccaca gaaacaagtt gcaccaggtc aattaggact
     3781 taaagctttg atgaatacac caacaatgag aaagaagttc gaagaggtgc ttcatgacaa
     3841 tgctaatgct tttatgtcga atgttatgac tcttgtatct aatgacagtt atcttgcaga
     3901 tagtgaaccg atgtctatca tgagtggtgc gttaactgct gcaacattaa atcttgggct
     3961 agataagaat ttaggttatg catatttagt tccattcaat agtaaaaaca agcaaacagg
     4021 aaaatgggaa aagaaagctc aattcatgct tggctataaa ggatatatcc aattagccca
     4081 acgatcaggt aaatacaaag cattaaatgt gatcgaagtt tacgaaggag aactaaaaag
     4141 ctggaaccga ctgacagaag agtttgagtt tgatccaaat ggtagaacgt ctgatgaagt
     4201 cattggatat gttggctatt tcgagttact gaatggattc aaaaaaactg tctattggac
     4261 caaacaagaa atcgaagctc atcggattgc taacaataaa gatcgcgata agacaaagtt
     4321 aagtggtgtg tgggcatctg attacaatgc aatggcacga aaaactgttt tgagaaatct
     4381 tctttctaaa tggggaatct tgtccatcga aatgcaagaa gccaccacat cggatgagag
     4441 agtccaaagg gttcaagaag acggcagcat tattgctgaa acagaagttg aagaagatat
     4501 tcctgaaaga aaagaagcag aggttatatc tgaagaaaat gaagatgtac aaactggatt
     4561 atttgatgca tctaatccgc cgttaaacaa ataatgaggg agctttctcc cttgcttttc
     4621 tagaggagaa atacgaatga acacaggata tgtcaaatta catcgaaaag tgatgaattc
     4681 attcgtttgg accaatcctt atatgtataa attatggaac ttgtgtttga tgaaagcgag
     4741 tcatgagaat cgcaagatac tttttaatgg aaaagagata caagtgaaca gcggagaatt
     4801 cgtcacaggg cgcgatgcta tcacatctga gatgaacgaa ggcgtgaaac gtgaacatca
     4861 agtgaacagc ggttctgtat ggagatggct aaaacaattt gaaaaaaacg gaatgttgaa
     4921 catcaaatca actacgaaat acagcgttat atcaataaaa aactggtctt tgtaccaaag
     4981 cagtgaacaa caaatgaaca tcaaacgcac aacaagtgaa caacaagtgc acacaaacaa
     5041 gaatgaaaag aattataaga atgaaaagaa taataaaaca tcatcgtcgc aaccacgcaa
     5101 aaagcgtgtt tacgacaccg actcagttta ctacattctc gcggaggagt tattcaaaca
     5161 gatttgtcag aatcaggaaa tcaaaaagcc gaatctgcaa agttgggcag atgacattcg
     5221 gaaaatgatc gagatcgaca agcggaccga aaaccaggta cggggaatga ttgaatggag
     5281 tcagcacaat gtgttttggg catcgaatat tttatctgct aaaaaattgc gagaaaaata
     5341 cgacacaatg gcagcgcaag caaatcgtga ttataaaaca aaacaaacta aaacgcttga
     5401 atacgagaaa tttagcacag atgagttgcc tatttgagag gaggcgtagg catggaaact
     5461 gttggggaaa tcatggaaaa gctgatacag aaagtgcttg tccaacgtgg cgaatgtcct
     5521 gaatgcggac agcctttgta tggatggcgt acgaagaacc ctgatggctc agaacgttgt
     5581 aagccaacct gtatgcaatg tgggtataaa gcattgcgag ttcaggaaga cttacaaaca
     5641 gaacgaattt acaacgagag cctgaaagca agagcaatca attttttcaa aggtggttct
     5701 gttgtgccta atcaagcgtt gtttgattgc acattgcaga attatcaaat tgtcgatcaa
     5761 gaaacaagac aagcggttga agtaaccaaa cgctttgtta attcagtctt gttaggaaat
     5821 ccaagtcacc ttgttttaac tggaaaacaa ggaacaggta aaagtcacct agcgatggca
     5881 gcggcttggg aagtcttgaa gcgatcaaac tacgataaaa aaatcttatt tatcgggtta
     5941 caggaaatgc tggatcaaat caaattttcg tacaacaatc ctgaactcag aaaaacgatt
     6001 gagggatcgt tgattgcaga tatcaaaaca gcggatttag tcatcattga tgatatcggt
     6061 tcagaactag gaaaagatgc atcagatagt cgagcgtttg gcataaacac gctaaattcg
     6121 ttcttggatg cacgacagaa cctagcaacg attatcacaa cgaacttgct tggtgaagaa
     6181 ctgaaaaaag cttacggtac gagaacaata tcaagaatgt ttgtcaactc tgatggattt
     6241 acgatggtat tttctcaaac agcagacaag cgcataaaac cagtcaaagg agcgatcgca
     6301 tgaatttgac taaacaagaa aaaagaatct gtcgtcagat aatgttgcaa attataggtg
     6361 atcgtgacat ggacgaatta tctcaacaag agagtcgaat ggttcggaag ataagtaagc
     6421 taattattta tcgggataca gaaagatttc tcactgtttt aggtaacgtc tacgagaatc
     6481 cagagttatt ggaggtagag taattgccaa aaatattaga tgcatgttgc ggtagtagat
     6541 tgttctggtt tgataagaac aattctgacg taacatttat ggattgtcgt cagcagtatg
     6601 aagaattatc aacaggtcat gtgatcaatg ttgatccaga tgtggtcgca gactttcgcg
     6661 atatgccttt tgaagatgat gtgttcgata tggttgtatt tgacccgcca cacttaagac
     6721 atgtcggcga aaactcatgg cttgccaaaa aatatggaaa attagacgaa ctttggccag
     6781 aagacatcag gcaaggtttt gcagagtgta tgcgagtttt acgaccttca ggaacattaa
     6841 tttttaagtg gaacgaggaa cagataccac tttctgatgt gttagaggct attggagaac
     6901 agccattgtt tggtaataaa cgaagcaaaa cacattggct tgtgtttatg aaaaaattca
     6961 gctaacggag gaaacaaagg aggaaaagta atgaagattg tattaaataa atgttttggt
     7021 ggctttgggt tgagccatga agcaaaaatg gaaattttta aaagaaaaaa tattgaagta
     7081 ttcccataca tcaataactt tagctatgac tcagatgatg aatacacgag atacacaggc
     7141 caaaagctag gttcgatgga ttttatctac tatttcaaaa aagacccgaa aatcgacaaa
     7201 gttacaggaa tttatagcga aattgaccga ctgtacggaa ttgctgatga ttccagtttt
     7261 tcaagtgatt caaacagagg tgataaggac ctcgttgctg ttgtcgaaaa actaggaagt
     7321 gaggccagtg gaccatatgc aagtcttaaa gtcgttgata tacccgatgg ggctgagtgg
     7381 gaaataagtg attatgacgg cgttgaaact gcacattatg gttttcaaac aggtagtatt
     7441 tagtccacta atgacaacta aagaggaggc agaaggatga gtgaagatga atactttgaa
     7501 cagtagtcta ttaacgacag agatttttaa ctggaaaggg gtgtagtcat ggacgcatcg
     7561 gaagtagtaa ataaaattaa tagtctcaaa gaggttttcg gagacgtcga agtagtgata
     7621 aacgcacaag gctatgacaa cgaatttttt aagcagatta ccgacattag cattcagcaa
     7681 ggaatggaag acgaagacgg aaactttatc gatgaagtag ccattttagt gacttgcgag
     7741 taagtaattg acagctaaag aggaggaagc ggaatgaaac taaaagacgg attttactcc
     7801 agcggccacg gcatcggcgg tttaatgcta gatatgccga caaagaatcc taaaacacgt
     7861 aagaaaccaa aattcaaagt cggtgacatg gttcgctgcg aagcagaagg attcatctat
     7921 ccatttcgtg gatatgtaga acacgtctat aatcactcag caatcattcg cattgaaaac
     7981 acgatggaat gtgataagtg gttagcgaaa agcaaagaga atttagcagt ggtgagattg
     8041 gtggacatgg aagttataaa atcttaattt tgctttttta caactatcct acaacaatga
     8101 gcgttattcc acaactggaa ggagaagagg caattgtgaa cattttagag aatatagata
     8161 ttaagcaaac aaggaaaaat gctagacgat tactaaaaag atacagaaat ttagaacgtt
     8221 tggtcggacc agtgaaaata gacttttccg tgatgactgt taccaaaaat ttgaaattca
     8281 caattgacag tcaaaacgaa gaaatcattg aagcgataag tactcgagat tcggttatcg
     8341 aagcattaac acggctaagt agaatccatt tccaggtact ttattatagt tattgttttc
     8401 ctaataagat gtcaatgtat caaataggag aaaaattagg atattccgat agaactatcg
     8461 agagaatgaa ggcagtagct ttggttgaat ttgctgaggc gtataaatca ggagaactca
     8521 tttcacgtac aaaataaaaa aagtcggatt cctccgacta tgaataatat ttccgacata
     8581 agtattatat cataattggg ggaatcagag gatggtactt ttcgatgtaa agaaatatga
     8641 gacaccaagc gcgaaagatg ttgatatgga acgcacaaaa cataatgtcg ctgtgtttct
     8701 ttcagcatat ctatcagcta gatgtagagt agggcaacat cgagaaccaa aagtgacagc
     8761 atcttattcc ttggttccac cttctacagc tgatcatata tttgaagcag aaagaatgct
     8821 gatcgataaa gaagaagcac aggaagaatt tgagtatctg cataaattgt ttgttcgagg
     8881 ctattcagcg atacagcatc cgcataagcc tgatgtgact gaaaggcgca agaagatatt
     8941 ctatgatcgt tatatcaatg gtctgcccat ttatgtaact gctcaaagga ataatactag
     9001 cgaagaatcg gttaaagtag aatcaaacag aattatcatc caatttgctt catcgttaga
     9061 actggttgct ttcaagtagc cagtttttac actttttata cccttttatt accaatttgg
     9121 tttccatttt ataccttttt tataccaatc acttacctat tcaacgttgt attatgatag
     9181 tgtcgaaaaa ttaggaaaca ggatcgacaa aataaaatgt aagggaggaa atctccctca
     9241 tcgtttaatt aagcttcgat aggcagcaac ggaaatatta agaataagga tgtgaatttc
     9301 aactccttct aaattgttct tattatctat catccgttgc tgtctattgt ttttttaatt
     9361 attcacacga taactaaagg taagtgaaga gaaatgattc cattaataat ttcaattttt
     9421 gcgctttgtc ttaatgtata tatgattgga tttaaaaatg ggcaaaataa aaaatagtag
     9481 cagccaagaa taattttata gtgtcactgt ggcggaaagg gtagacgcta agcatgtgtg
     9541 ctaggtcaat gcttcggcaa ccatgcaagg ttcgattcct tgccagtgac tttggcaacc
     9601 gaggcatcgg cggtttaaaa atataggggt gcgcaatttc gtacgcgttt tgtgcatcgt
     9661 gtaggttgct attacataac tggttaggtt agattgagat ttgggattcg gtacaaatga
     9721 atcgtcaaat gtctcaagca caggatcgga aacgtccctg cctgtgcatt acatattaga
     9781 tcactctttg agtggtcttt ttctttacat aaaggaggct acataatgag aaactactgg
     9841 tatgtatcgc taactaatga atatcctcga accattgatg attgttcagt gcgtgttgtg
     9901 cgttctgtac aaatcaaagg gaagtactct attgtcgaaa tggggagaga agcagaacca
     9961 tgtgagattg atgcgtgcaa gctcgtttat tgcggtcatg gtttctatga tgaaccaaac
    10021 attcaaaata atattaacaa gaatttgagg gattagaatg caagaaatgg cttaaaaatc
    10081 tccaatcagg acaaatcaag ttagacaaag tttccgattt aaagatatta attgaagcag
    10141 atctaatgtt gaaagatatt gaaaattaga aaacaaaact caacctaaac agaatgtgag
    10201 gtggtgtgta ttgaatggca agaaaacgtg atccaagacg tgatgaagct aagaaaattt
    10261 ggctagagtc caacggagaa aagcaattaa aggaaattgc atcagaatta aatgtttcag
    10321 attctcaggt tagaaaatgg aaatcgcaag acaaatggag cgctgaattg aaaagtaacg
    10381 ttaccaatgg caaaagtaac gttactaatc aaggtggcgc tcctattggt aatcaaaatg
    10441 ctaaaggtaa caaaggaaac agcagagcat caccacctaa gagaaacaag aatgctttga
    10501 aaacaggcga atatgaaaca atattctttg acacgttaag cgatgatgaa aaggacatct
    10561 attcaagttt ggatgatgat ccttcttttg ttttgtctga ggaaatacgg ttgttaaaga
    10621 tacgccagtt acgaatgatg aaacggataa aagaagccga gtcaggttta aacgatgaag
    10681 aggttgatcg cctgcaacaa atgcgaaaga ttaaaacgcc aatcgaaaag gatggtaaaa
    10741 agctagaaat caagcgtgag gttatgcaag atgtgcagat tagcagaaaa aaacatcgca
    10801 aaattgatga tattctttca attgaagatt cattgactcg gattagcaac cagttagcta
    10861 aagccatcaa gcaaatgaat gaactttata tgaatgaata cagaactgat ttaattaaag
    10921 ctcagactga taagatccaa gctgagacaa atgaaattgg cggaaataat tcaggtgaag
    10981 aaatagaaga atggaaacag gcagttttaa atgccgcaaa caaacgggcg gtgaaagaaa
    11041 atgaataatg aatttattcc ttttgctgat attggttctg ccattgatta ttactatgat
    11101 aaaccagtag ctttttgcca agatattttg catttgaatc ctgatgaatg gcaagaaaat
    11161 gttttaaatg atttagctga attttcaaag gtttctgttc gttctggtca aggagttgga
    11221 aaaacagcat tagaagcagg agcaatactt tggttcttaa cgtgtcgacc ctacgctaaa
    11281 gtaatagcaa cagctccgac aatgaagcaa ctttacgatg tactttgggc agaggtagct
    11341 aaatggttaa atgatagctt gatcaaaaac ttactgaagt ggacaaagac caaaatttat
    11401 atggttggtg attcagagcg ttggtttgct acggctagaa cagcgactaa accagaaaat
    11461 atgcaaggtt ttcacgagga ccatatgttg attgtggtag atgaagcttc tggtgtgtct
    11521 gatccaatta tggaagctat tcttggtacg ctatcaggtt ttgataataa gctgttgatg
    11581 tgtggaaacc ccaataatat tgaaggtgtt ttttacgatt cccacaattc agaccgtgat
    11641 aaatacagag ttcataaagt atcaagctat gatagtaaac gtacaaacaa agacaatata
    11701 gaaatgattc ttaaaaaata tggaaaagaa agtgatgttg ctcgtgtccg tatttttgga
    11761 gaatttccca aaggtgcgtt ggattcattt atcagtcttg aaacggttga attggctaca
    11821 gaaaaacaaa ttagtgattc tttagtcaat aaaacaacgg ttgctcatat tggtgttgac
    11881 gtagctcgat atggtgatga ttctacgatt ctctttccta gaattgctac cagggcattg
    11941 gagtatgaga agtattcaaa acgtagcacc atggaaacaa caggatatgt catcaacatg
    12001 gccaagaatc taatgagtca atatccgagt attgataaag tgatgattaa agtcgatgac
    12061 actggtgtcg gaggtggtgt aaccgaccgc ctagaagaac ttatagaaga caaacattat
    12121 ccttttgagg tgtttggagt gaataacggt tcaacatcag aagacgattt ttacgataat
    12181 ttaggtactc aactatgggg aaacatcaag gaaatgttag aagaaaatat gacagcaaat
    12241 cttaacggag aacagcctgt tattgaattg ccttctgata gttcgttaat caaagaatta
    12301 agtactcgca aattcaaaat gacaagtaga agtcgtatac gtttagaaag taaagatgat
    12361 atgaaaaagc gaaatattgg tagtcccgat attgctgacg cactggcttt agcgttttat
    12421 gagccaccaa gtcactatca atttattcaa ttttaggagg tgagcttttg attagtacag
    12481 tattatcttt agagagatat aagaagctac gtgttaaata tgcaacacaa attgaagatg
    12541 gaatgtttga tccgaatggt tttatagaag atatgaaacc attttttgct gatcgcgaaa
    12601 gaaaatatct agcttatact agcgaaaaga atgaaataga taatagacca aagcctaaca
    12661 ctgatattgt aaaagttaat aataaacttc atgctggtat gtattcaatt gtcgtagacc
    12721 aagcagtcaa tcattttact ggtataccta tcaaatggga ttacgatgta tcggaacaaa
    12781 aaagaacact cattcaaaga ttaaaagata aattcttaaa aaatgatata gaacttccga
    12841 cagttccaga agcttttaac aaattaacaa gcaatcttga ttccatgaga ttcgcaatgc
    12901 ttgattctga aactgcaact tttcaaggag cttgtggagt agctttccga ttgttagagc
    12961 ctgtggaaga agatgatggt tggaagttaa gagcaagtaa tattgaacct tggagagcag
    13021 aaagatatgg aaatgccgga atctatatca aagaaaagta tgactcttac cagaaaaaat
    13081 tttttcaaga aatgaaagtt atgacaagaa ataaaattct gacatatgct tgctatggtg
    13141 atttgaattt tgttacaagc ggaacgttca aaaaaattga tgagaccgat aaccctttgg
    13201 ggacgattac cttggcagaa tttaaaaata atacgaatcg ttattgtgat tttgaagtag
    13261 ctgaggaaat tggagatgca attgatcgtg ctttgtctga tcaacaaaac gaaatcgaac
    13321 aatttaagct tgcgtacatg cttgttactg gcacaacaat gagcaaaggc actgctaaag
    13381 aaatgatgaa tcagctaggg attatcaact taaaagcccc tactgcaaaa gctgaatatg
    13441 tcacaaaaaa tttagcaaaa gatttcaatg aatatcatat ggatctattg aaaaaacagt
    13501 tttacaccat ttgtaaagca atcgatttta acgatgaggt gtttaaatca aacagttctg
    13561 gagaagctcg caagtggcaa atcattagcc tagaagcgaa aacaaatact aaagagcaat
    13621 attttagaga aggattgaaa gaatgcgcag aaacaattgc tgcttttctt aagtttcatg
    13681 acaaagtaga aattgagcct gaaaaaatta tttttacttt ctctagatct ttgccaactg
    13741 atttaagtta tttagctgaa gctttaccta aacttgcacc atacgtatcc aaacgaacta
    13801 ttcagagcca aattccattt gtaacagatg ttgattatga aaatgaaatg atggaattgg
    13861 aaagtggaag tgcttatcca gatagtgaat acaattttgg cggaggtggc aatagtgacg
    13921 atagaaacga agtattggac caaacgtcga gaactggagg atcaagcaag gctcaaacaa
    13981 gaaaatcaga cacttaaaaa attaactagt gtatttcctg aagcactgaa agagatacaa
    14041 gcaaaactat tatcacaagc tgacttacac aatatcactt atccagaaat gatggagttt
    14101 tacagtacaa gtaatcagaa aaaatatcgt gaatatgtgg aaaaaaatta taagtcatta
    14161 aaaatgtatg atgcaaaata caaagagttt atcgatgaat tttttccacc atttgactat
    14221 gcaaaagtca atcgcttatt acaaatacga tcagatgtat ttaaaattct tgcagaatat
    14281 gcgatggatg cagatgtgaa tcaatatttt tctgatcgct tagaggaaat tcttcaaaga
    14341 acatattctt ctaatgctaa tgtttttgtt caacttttaa acgttgatat accaaattat
    14401 ttaccagaaa atgaaattaa acattatttg aactatccat ggtcaggaaa gacattttca
    14461 agaagacttt ggggaaatat ttcatcgctt gaacagaaac tctctaatgc tattgtaaaa
    14521 agtgttgcta gtggagaagg cgttatacac gcattaaaca ccatgagatt agactcagaa
    14581 atttgtgaca tgtttaagtt agaagaatca aagtataaca aagcgataga aaatctcgtt
    14641 cgaacggagt atgcaaaatt tgcacaagat ggtattgaaa aatcatattt agaaacaggt
    14701 attgaggaat acaacgtatt gactgcaaaa gatgagagag tttgccggat ttgtggagga
    14761 aaggcaagta agaatcccta taaactgaaa gatgctgtca taggtgaaaa tcgagcacct
    14821 ttccatagtc gttgtagatg tacggatgtt cctaatttac caaaattagg aaaggatatt
    14881 gatgaagaat atgaccgttt atttggcgat ttattagatg agtttgcaca tgattctttt
    14941 ggaattaatt tgaaacggag gaagtagaat gaaagatttt tttgaagcag tactaacaat
    15001 taatgtaaat gctgatattg cagaagccta caaaacagct attgagtctg agaaccatcc
    15061 taatggcttg agagaccatt ggaatggcaa ttatgcctac gtggttattg gcgatcaaac
    15121 tgttaattat caagataata ctccagttga taagaatacc gttaatttaa cgattcaatt
    15181 attatctcat tcattaccaa atttaaaaga aacagttgat tggtatgaaa agatgggatg
    15241 tattgttgtt agaactgact acaaagaagg aaagtctagt aattaggctt ttttattttg
    15301 tccgaaatga cgttaaacta gcgcaatact gggcttggtt gaatggtggg gcgcaactat
    15361 taaaactcaa agcaatgcgg ggcgtgaaaa cgaatcgtgg ggcgaaagga gaatgagcat
    15421 gaaacacaaa tcattaatgc caatgaattt gcaatacttt gccgaaggtg acgatcaaaa
    15481 gttttctttt gatgacttca aatcttttgt ggaatccaat gaagaagctc aaaaatttat
    15541 tcagtcacaa tcccaaagtg ttgccgacaa acaattggaa gcttggaaac aaaataactt
    15601 agataaaatt aaacaggata ccatcaagga atatgaggaa tctaagaaaa acaagtcacc
    15661 tgagcaaatt caactggaaa aactacaagc tgaatttgaa gcagaaaaag cgttgcgtgt
    15721 gacaagtgat aataaagcat ttgttgcaga acaaattgct ggattagaac tagatggaga
    15781 gttaaaagag tctatttctc aatttatgct aaataatctt gttagttcgg atacagattt
    15841 cactaagaat gctgttgaag gttttacaag tgttttgaat gcaattaatg agaaacatgc
    15901 agatgcatta aaagaactac aaatgaagtc tgcatttggt ggaactcaac aatcgaataa
    15961 ccaggttcag cagaacaatg aaacattaac aaatccagaa gaacaattag gacaaattct
    16021 tcaacaattt aactaggaga gtgaaaaatt atgaaaaaaa catctttaaa taatttagag
    16081 tatttggata tttcaccagc gattaatgct atgcaagtac caaatacacc ttttttaagc
    16141 tatttacttg gtgctggaaa aacagagcca gcaaactcga cagaaattaa atggcgtgaa
    16201 tatgatatca acaacgatga ttcttctgaa aaacttgagg gcggagaata tccagatgct
    16261 gaatcaggtc gaacttggtt taacaactat actgaaattt ttagaaaatc aacctctgta
    16321 tctggtacat tagatgctat taatgtgaat ggagtcggaa atgaattaac taatcaagta
    16381 gcactacgtg gtatggaaat gaaaattgat ttgaaccgaa aattgattac tggtgtaaaa
    16441 gctgatgaaa atagttctaa aggtcgtcga atgaatggga ttttgaactt gatcaattca
    16501 gcaaataagg cagaaacagc cactgcgggt gcagtaacaa gaaaagatat cgatacttta
    16561 tttaaattga tgtatgaaaa aggttatatg ggagaaaaac tatgcttgat ttctccagat
    16621 atgcaggagt taatgactga tgagttagat ggaaaatcaa caaaaattgt tcagttcggg
    16681 gaaagagtaa cttttggatt gcaaattgga aatatcgtgt ctaattacgg tacaggtatt
    16741 gctctactag aaccatcatt gccaaaagga acaattgccg caatcgatac taattatgtg
    16801 aaactacgtc cattacgtga atggagagca gaagaacttg caaaaacaac ggattctaga
    16861 cgtatcggtc ttgtaggtga atactctctt gaatacaatg cttcaaactc tggggcaatt
    16921 ttaaatttaa agtctgaata aaagggagtt agtactccct ttttttgata ggaggaatta
    16981 cagtggtaaa aaaagatgaa actaaaaaag atgaagtcgt gaaatataga gtaggtaaaa
    17041 ctaaaaattt tgttgggttt gttcatccta aaactcgtag atttatcaca gcagattcaa
    17101 ataacgaatt tatcatttct atagatgata aagaagcaat tgcaattttg gaagatgcaa
    17161 ttgatgttaa tgaaatttag gaagtgatct gatggatgaa tcgctaaaaa cggaaatcat
    17221 tgagtctaca aaagaagatt ttccagattt gagtgaagaa cgcataacta atttattaga
    17281 aataattttg ctagaaattg aatcatacaa cacttgtaaa aatgatattt catgggaaaa
    17341 gttaaaaagc gtgattagtg aagtgttgta taaaataata aaaaatgaat cagaaaaaac
    17401 agtatcttca gttagacgtg gcgatacaac gattagttat gcttcaacaa caaatgatgt
    17461 tagtgagtta cttctaggct atggtgattt gatacgaaga gttattggtt gtggaggatt
    17521 ggagtttttt taatgaatga agcagatatt ttagaaacta cttatgaaga tagttgtatt
    17581 attgaaagac tgacggacat tgaagattct aatacaaata ttactattca agattacaaa
    17641 aaagtatatg ataatcctat ttcttgcgct ctttcacaag gtcaaatcga tggactagca
    17701 gtcatagaag atggagagat ggtaaatgtt tcaactgaca catataaatt atttgttcat
    17761 cctaagatta aactaaaaaa aggagatcga ataacaataa ctcaaaaggc cagtggctta
    17821 attttttctc tatttgctac taagcctttt tactatccta gtcattgtga agtgaatttg
    17881 ataggaagtg agaaaaatgg gtaatctcaa atatgaatca aatgcagaaa agatcattga
    17941 gaattttaaa aatatgactg taattgctca aaaagaagga atatccttcg ttaacgattc
    18001 aatgaataaa gtcgttagtt tagctaaacc attaactcct gtaaaatcag gtaatttaag
    18061 gcgaggatat cgtgtagtaa aagctagaaa actatcaagc ggtcgaattg ttggagcagt
    18121 tataaacaat gaacattatt ttaaatatgt agaagaaggt cataggacta aaaatggtgg
    18181 ctttgtcaaa gggaaattca tgttaactcg tgcaacgaat cttgcaaata tgtcttatat
    18241 tcctcgaaga tttaaacaaa tggcaataaa aatcgttaag aaaggaaagt aacatgaaag
    18301 atgaaatcat tgctgcaatc agtagcaaat taaaagaaat ctatcctgat gggacaatat
    18361 atcttgattc agttatgcag tcaactaaag atttttattt cgttttatca gtaatggaat
    18421 ctggaactga aaatgtagga attgatattc aaaatgtttc tttcttaatt gatattgcat
    18481 tgattgataa taaccctaat agaaatttaa ttaatgaatt agtctcacgt tgtgggactt
    18541 tttttaatac gattacaatc gatgagcaaa tattatttcc aaaagcctat ttacctgatg
    18601 aagcagatgg tgttcaacat attcgtttta cattagaatt tccacaatat attgaatgga
    18661 gtgaaagata aatgggagaa aaaagaagta aaaccggaat tatttctgtc gaaaaaccta
    18721 cttggtttcc tttggaagat gagacaggga attttccggt atatggtact gcgttgccaa
    18781 tgggaacagc agtaagtatc aaaccaacag ctaattatga aacaacacaa gattatgggg
    18841 attcagtggt tcaagatcaa tttacagctt ttggtggtgc agaagttgaa ctggaagcaa
    18901 atggatatac tcataaagtt ttatctacaa ttactggagg gaaaattgtt aaaggtggtg
    18961 ccttgcgttc tggagaagat attgcgcaag atggtgcgtt tgcatataga cgtaaaaaat
    19021 cgaatgggaa ataccgttat acggtctttt ataaaggaca gtttgctcta gattcagatg
    19081 aaacatcaac gattgaagga agtaaagtaa gttttactca tccaacttgg aagggttcat
    19141 ttgtagatgt accaggactt ggatatatgt attcagttga tgaagatgat gaaggcgtgg
    19201 ataaagcaat gatcggaaat tggtttacaa aagtagcaat tccaattgaa gaagcagaac
    19261 tttcaggagg tacagaataa tgtcaaaata tcaaacaacg attaaattaa cgaaaaaaga
    19321 cgaagaaggt aaatatgagc aagtacaatt taaatctgct gaatttttac caggaacagt
    19381 tgtagaagat gctgcaggag ttatggaaga aatgcaaaca gcgactgata agcaatcagt
    19441 taaaaaagct ttaagtcgtg cttattcatt tattgcagat actttatttg agggacaatt
    19501 tactggtgaa gattattgca aaggaattga tgctcgtgag attgcctcat tgacaggaaa
    19561 attattgaag tctgttactg caggttttga tgaaacttat acagagacga aaaaaaagta
    19621 agtgaggcgc tcaagtcacc ttcatttaag tattcgatta cttaccgaga attagatata
    19681 aagacgcaat tactcgaagc aggttggacg ttaccagaaa ttgaacgtac tgacttggat
    19741 gagttaatgc gtctttttgc ttttagagat gcggtaaaag agcatgaaga tgttgagtac
    19801 tatgataatt tcactcaatt ttaggaggtg atactttgaa taacgatgac ctaattctga
    19861 agatgatact agatgaatct ggttttactg ccggtatgaa taatgctgtc aaaaagttga
    19921 actcttttga tgaaacaatc gaaagaaaaa gtagaaatag tggcagttca ttaggtagta
    19981 tttggaaaat atttgctgga agttttctag ctagcggagt aactagaatc gtaggagctg
    20041 gctttgatct aatcaagggt tccataagtg gagcggttga tcgagtagat acgatgaata
    20101 acgctctacg aaatttccaa aatatgggat ttagtaattc agaaattatg aagaatatcg
    20161 gaaagaatgg gcttttatct caaggtattc aaggacttcc tactgctttg aatgatgcga
    20221 taagtcatgt tcaacttctt gcttcttcta caggtgattt aactcgttca actcaaatat
    20281 ttaaagcttt aaatgatgga attcttggtt tcggtggttc aactgatcaa gttaatgaag
    20341 ctgttattca attatctcaa agtttctcaa acggaaaagt agacgcacaa acttggaact
    20401 caatgatcaa cgctcaactt ggacctactc tttctgctat tgctaaaaag atgggaatta
    20461 caatgggaga tctgaaagaa ggtttatctc aaggtaagat ttctgttgaa gaattccaaa
    20521 atcaattaat agaaatggat accaaaggtg gcggaggact taaatcatta agtcaaatcg
    20581 ctaaagattc tactaaggga attaaaacct ctatacaaaa tgcaaaaaca gctgttacac
    20641 gtggtgttgg tgaggtaata gaaggattaa ataaagcatt agtagattca gatttaggtg
    20701 gatttaaggg aattattgat aaagtgggca gttcaatgga atcgttctta aaagtaattg
    20761 ctgcaaatat tcctacagca gtatcatttt taagtaattt atttaacgaa attcaaaaat
    20821 ttggttctgc tttgaaattc atgatgccct ttcttgttcc tgcagctacc gcttttggag
    20881 cgttcatgtt tcaacttaaa ggtataccag caataataaa aagcttcaat aattttaaga
    20941 atgccataat tggcgttgga aattcattaa agattatggg ggcaatagct gctgctaatc
    21001 catttgtttt aattattggt gcagtcgttg gtgcaatagc tgttttcggt tactttatgg
    21061 caaccaatga agagcttaga aacaaggtta tatctatttg gaatgatgtg aaagattccg
    21121 taattggtgt attaaaaaat ataaaggact ggggaattga tacttggaat tctgctaaag
    21181 aaatggcatc aaatgcagtt gagggtgtca aagatgcttg gtcaggaaca aaggaatggt
    21241 tttctaacac ctggaaaggt attaaggatg gggccacagg tttatttgat aaaacagtag
    21301 agacttcaag aaatgcagtc gatagtgtaa aaaatgcatg gtctaatatg aagaaatggt
    21361 tttctgatac ttggcaaagt ataaaagatt cagccagaga aaaatggaat ggaatcaaag
    21421 gttctattat gtcagttgct ggtccattga ttacaggtat aaagaatgca tttttacatg
    21481 ttacttttta tctagagacc ttatggaata atttagttga gattggtaaa aacgtctttg
    21541 agattttaaa aaatgttata cttgcgccgg ttttgtttat tacctccatg atttctggtg
    21601 gatgggaaga aacaaaaaat aatatgattg ctgtttggaa taatattaaa gaaagtgcca
    21661 taaatatttg ggaatctata aagaatatat ttgtaagtta ttttacaaat atttattttg
    21721 ctgcactcaa tatttggaca gggttcaagc ttacattgat aaatatttgg aatgaagtgg
    21781 taaatcaagc taaatcgatt tggttaaaca taaagtattt ttttattaat ctttggattg
    21841 atataaaata ttttgcaatt cagaaatgga ttgaattgaa gttttctatt attcaaactt
    21901 ggatcgattt aaaatataat gccattacta cttggaataa tattaaacag tttttcaaag
    21961 atacttggaa aaatattaaa gatacggcat acaatacgtg gatttctata aaaaatacca
    22021 tgattaatac atggaataac attaaggact ctttctggaa tattgttact ggaattgtta
    22081 attctgctga aaatgcatgg acaaatctaa aaaatggtgt ttcaaatgct attaatcggg
    22141 taaaagaaat ctttaattcg ttaagggaag tcaatttatt tgaaattggc aagaacatca
    22201 ttgatggact tataaatggt gtaaaagaaa agtggaatgc tttgaaagat actatcaaag
    22261 gtattgcagg taatatcaaa aattggatta aaggagcttt agatattcat tctccatcaa
    22321 gatggatgcg tgatatggtt ggtaaaaata ttgttcaagg tattatcgtt ggtattgata
    22381 aagagcaaag taagttagat caaaccatga caaatttagt aaaaacaccg tctgttcaac
    22441 ctgtagtaaa tggaataagt cacctaccaa ttacacaaac aaagcaaaat atatctgcta
    22501 gtgaaacaaa agaaattcat ttacatttaa atgtttatgg agatttacct gattcaatgg
    22561 ttcggaagat taccacaaaa attaaaacag aattgacaag acaaatgaaa cgagatgctg
    22621 gtgcagtagg aggaacatta tatgcaactt aagagaggac aattttttat taatcaacat
    22681 tattcttctg aatttaatgt gtatattcaa aacaggcctg cttctgtttc agctagtcga
    22741 gtaattgaat tgagagagcg agaaggaaat gattctatca ttattgataa aggctattac
    22801 aaaaatgtga ctagaaagat tgagtgttac tataaagcac cttcaattga tatggtgcag
    22861 gagtgggaag atcgaatcac tgaatggtta gatatgtcgt cttatagtga ttttattctt
    22921 tactatgatg aacaatatat ttatcaagct gtagtaatag aaggacctga atttaaagga
    22981 acaagaaaaa caggaaatat tgtccctttt gaatttacag tgagtattcg accatttaag
    23041 gaaaattaca atggtagatt tactattcga caaacagaaa cttttgaaat ttataatcca
    23101 gagaagtatg cttcaaaacc gcttattaaa ttgagtggtt ctggagatgc ttctttttat
    23161 attaataatg ataaatatga tttgaaacta ttagatagag aattatatat agattctaaa
    23221 ctagaagagt cttatcgaaa gctagatgac aatttagaac atcaagatca tgtcacttta
    23281 tttttagatt ttccatttct ataccctgga aaaaatgaaa tcaaatggac gaataacata
    23341 cattcgtttg agataatacc aaggtggtgg agaaaagtat gataccaaga atatatagtc
    23401 ctaccgagac agatttttct acgaatggtt tagggatttt gaaggacacc acaaaatgtg
    23461 agatatatga agtagcaaat ggaaaatatg aattagagtt ggagtatcca ttaggtactc
    23521 gatttgatga atattttgaa aatgactatc aaataaaagc aaagtcaaat gatcaagaag
    23581 agtatcatat cttttttatt gatgataaag atattgatac ctttttagat accgtaacta
    23641 tttatgccca gagtcgtaca aatcggcttg gaagacgagc ggtcactttt gctgaagtcg
    23701 actctaaaac tggtcgagaa gcaatggcaa ttattgaaaa caatatggat aaaaaatcag
    23761 atatacgctt atattctgat attacaactg tatcaagtac aacttttgaa gcaagaaatg
    23821 ttttaaattg tattgctggt gaacaaggat ctttacttca gtattggggc ggagaaatta
    23881 aacgtgagcc ttttaagcta tctttattaa agcgaagagg tcgtgataat attggcacaa
    23941 tccgatatgg aaaagattta tctgggttaa aagttaagtt agattggaca ggcgtgaaaa
    24001 caagaattat tccgtatgct gatcctcaaa gtgatgcagg tacaactagc cgaatttatg
    24061 gctcgccagt agatagcgaa tatattaata attatcctga tgtatataca gaacacgttc
    24121 aattcacaga agaacaggga gttaaagacg ttaatagctt aaataaaata gctaagaatt
    24181 atttcaaaac aattaatcca ggctgtgata aaccgaagat ttctattacc gttgagttcg
    24241 ataaattaac ggacaccgaa gaaggaaaag aattcgctaa gataagaaat tatgggttat
    24301 ttgatacgtt taaaatatat catcgaaaat ataaactata ttttgaatcg aaagttagcg
    24361 gtgttcaata tgattcatta tctgaaaagg ttttgaaatt ggaagctggg gatgctcaag
    24421 ttgcttttta tcaacaacaa gctgttacta ttcaagataa attaaaagat tatgctacga
    24481 ataattatat gagtagtttt aatgattatg tgtcctctat gattgcaggt caaggaaatg
    24541 caggcggtta tgtagtttta tggccaaaag aaaaaccttc aaatattttt ataatggata
    24601 gccctgattt aaacaaggca aaagaagtat taagaatgaa caaaaatgga attgcctttt
    24661 ctaaaaatgg ttggaatggt ccttttaatt ccgcttggac attggatagt atattcaatg
    24721 ctaattttat tcaaacagga ttaataaagg cagatatctt tcaaaactct tttaataaaa
    24781 ctggagatgt attgaaatta gttaatggac tacttcaaat ttggaataac aaaaagaaaa
    24841 taatggagtt aaccaaaaaa ggaatggagt tttggaattc taatagttca attggaacga
    24901 ttggaacaac tgattctgct ggtaatcctt ttcctggcgc atccactcct acaccgttag
    24961 aagataattc tttagttatt cgtacaaatg gagacggcaa atatattttg atttctccta
    25021 aagtaggaaa aggattagtt ttattaggaa atggtaaagc aatttatttt ggagacttag
    25081 atgtacaagg aaaacttaca gttaaaggaa aagaaataat tcctggacaa ggtggtggtc
    25141 catcgggagg tggagaatct actggtgggt atcctagtga attaaaaaca gatgcagaaa
    25201 agagagcttg gagaatttac gatattttgt gtaataacgg atttacgaaa caatctgcat
    25261 gtggaatatt ggggaacatt caacaagaaa caggaggaac ttttgatcct gatactgttc
    25321 aaataggtgg accagcatac ggattagttc agtgggatgg ttcttcatat cctcttgttg
    25381 gtccagcaac atgggatgga aaagtttatg ttcaaaactt atttaatgct gcaggtatta
    25441 aagaaccaat aacgagctta gatgcacaag ttcgattgct tatttggaca tttacaaatg
    25501 gacaatggat gggcgtagta caacctacga cggttgatgg atttaaggcc tgtactgatc
    25561 caagacaagc agcatatgct tttgaacgaa actatgaacg tccggcagcg acacatcctg
    25621 aacgtcaaga ttatgcagtt aactggtata acaaatttaa agatttaaaa ccaggaggag
    25681 ctactggaga agcaggacta aaacatttag agtctttgat tggacaacga attggcaatg
    25741 gtcagtgtta tggcttgtct gcagaatatt caggatatct gggtggttgt ggcatgggtg
    25801 ctggaacaaa atatggttta actcatgtga taggaaatac ttctgcagca tctgatattg
    25861 gtattgccta tgattggtct gctgttggtt ggaaagtgat tcaaaaccct agatatgatc
    25921 aattagtagt tggtgcaatt attaattggg caagaggtgg acaagtgggt tcatggtttg
    25981 cagacggaac ttatggacat actggcgtta tccgaggctt agctaatggt cgtatgcaaa
    26041 cttatgaaca aaacacagag ttaggtatga tttgtggaaa gttagatcgc cagtattata
    26101 gtgcaagtgc aatttcttcc attgtcatac caccaaaata gggaggtgat ttaatggcaa
    26161 agtggaatgt catattaagt acaacagaac cctataatta cgtggggatg attcaagttc
    26221 gacaaggcaa taaaaacact gaagttatgg aagctacaat tgtcgaaaat ggtcttccat
    26281 atgatttatc agaatgtaag gtatattttg aatcagttgt tggcggaaaa tatccagtcc
    26341 aattagaaac aagaattgtg gatgctaaaa aagggaaaat caaatatatt tttgataaat
    26401 attccatgca gtgtttgcat cgacaaacag ccaatttcat tatatttaaa ggagaagact
    26461 tgattggaac aactcaagac ttctcctatt ttgtcatcaa tgctgtttca aaaacagaag
    26521 gagaaatggg ttcttattgg caatcaattg aagatttaat tgctgatatg acagatttta
    26581 ttaatgaaaa taaaggcgat tttacggact ggatgaatga aaggaaagaa gaatttgatc
    26641 gctggagaga agaacaggag aatagtttcc aagattggag agaaggacaa gaatcagatt
    26701 atttgtcatg gtttgaatca atcaaggata ttttgaagtc tatcgatcca ggtggagtaa
    26761 tgttagccga attaatggat gcacgtgtag atcttcaagg tgtacgccat gaatcgattt
    26821 ctgaacgttt cctatccgat ctaaattatt tgtatcaaaa aatgaaagca gcacttttca
    26881 cgattgaata cggtgaaatt gaagtgactg atattcttca agacgatctc ttttcagata
    26941 atcacgaagt cgaaaaaatt gaaacagtag aatttccaat tgaagaagga gctttgatta
    27001 ttgcaactgt tgatgatcca aaacagaatg ttttcactct tgagaaagtt ggggtgattt
    27061 gatggttaaa gtaaagagaa tgatggagac tgatgaatct ggtgttaaac gtcaatttat
    27121 gccaattaca catgtttctg cagttttagg cttagaaaaa atgatatcag gacaatcaaa
    27181 agttctttcg gttaatggta aatatggtgc agtcatttta acgaaagctg atttaggatt
    27241 agagaatgca attactgaac taccttatgc aagcgagaca agcgatggta ttcttactgc
    27301 tgaaatgttt caaaagatcg taaatggaga aggtggaaca tatattttgc cgattgctac
    27361 tcctgaacaa ttaggaggta taaaagttgg ggaactctta gaagttactg aagaaggtgt
    27421 tttatctgca actaagcaaa cagacttcaa tttctctgaa gaattaaaag gaaaattaga
    27481 atctctaaaa atattaaaag caggtgcaaa tatatcaatt gcagaagatg gaacaattag
    27541 ttctacaggt ggtagtggga ctggtggtgt taatcagtca tatgtagatc aaaaattcca
    27601 agaagctgta aatcaagcag aaaattacac aaacgaaaga attccaaatt ttacttttga
    27661 aaaaattggg gaggtataga gaatgacaga tatcgtaaaa ataaaacaaa gcaatgtaca
    27721 ggtttatcca caaactcatt ggaatgctat agaaggcaaa ccaacaacgg tgaagggaga
    27781 taaaggcgat cctggccaag cagcaacaat taccatagga accgtttcta gtggttcaac
    27841 cgcttctgtt acgaatgtag gaacatcatc tgcagctaga tttaattttg ttttaccaaa
    27901 gggagataaa ggtgatcctg gaataaatgc cacaactaca gctgtagcta caactacagc
    27961 aaatgggttg atgtcgtcca cagataaaac taagttggat ggaattgcag ctggagcaca
    28021 gaaaaatcca ggtaatgcta caactacgac agcaggttta atgtcagcaa ccgataaagt
    28081 gaaacttgat ggattagcga atattacatt tgagaaagtg gggacggttt aatggctgat
    28141 attgttcaat taaaagaaga cggagttgct aaatacttaa aaacgcatgt agaagctatt
    28201 gatggtaaag aagcattggt gcagacagat ggagaccaag ccattgcagg acataagaat
    28261 ttttcaggtt ctgtaacaat caataataaa cgtgttttga cgactgatga taacagatat
    28321 gaagtggtaa atctggttgt cactaacggt aatacaggga cagcaaagct ttatcgtgaa
    28381 ggaaaaacag tttctatata ttttgtggct ttaaacggaa aaagtagtgg cgggaatgat
    28441 tcggttattt taactgttcc agaaggctat cggacaccaa ttagttttga acaactggtt
    28501 gggtcaatag accgttctac tttgaacagt gctcaactat ctattggcgc agacggagcc
    28561 attaaatgga gaagaaattc aagttatgga tcagcttatt catttgttat cacttattca
    28621 atttaaggga ggaaatctaa tgaaagtagt ttacaaatca atcaagcctt acggattcga
    28681 gcaaatcatt ttgaacaatc aagaaaatat ccctgaaaac tgtacagaga ttaaaccacc
    28741 agttcctaac tggagaccaa gatttgattt tgataaaaaa cagtgggttg agttagctac
    28801 tgaagaagag aaaaaaggga atgctgttga tagcgtagat gaattggcta atattaaggc
    28861 attatacgaa acgctgaagg cagaaaatga tgaattgaaa cagctaaatt ctaaagcaat
    28921 gctaaacaat gtagcaatca agcaagaaaa tgtcttattg aaagaaaagt cagaaagttt
    28981 agcgcagttg aattcaaaaa cgatgcttgc ttctgtacaa aataccaagg aaatcgaaga
    29041 aatcaaaaaa caattacaag gtgggaagta acatgtattc atatgaagat atcaaactaa
    29101 tgtatgactg gggctgtttc actaacgaac aagtaatggt tttcgttcca ttatgcatta
    29161 cagacgaaga agcagataaa atcattaata aagataagag cgcatcttaa ttgatgcgtt
    29221 tttatttaag gtaaaggagt tgtcacatga ttaatttagg ggaatgggga atgatagcag
    29281 gatcaataac cgctatcgtt tctttgattt tattagtaat tagaccgatt gctgcatctt
    29341 tctcgaaaat tactgagact ctttcaaaag taagccacaa tttagatttg ctgactaaag
    29401 atttagaatc gagcaaatca gatcgattga tgattcatga agaactaaag aaacacgatg
    29461 aaagattaga tacacataca gaaaaattgg tggaacacac gcaacaaatc aaaactttgt
    29521 ttagggagag aagaaaatga ataataaaac gttcgaagta ctaaaatggt tcgcactggt
    29581 aattattccc gcactagcta ctttcgtggg gttagttggt aaagcgctca attggcagta
    29641 cacagatatc tgtgttgtca tcattactgg ttttggcgcg tttttaggga gtgtgttggg
    29701 tgtatcaaat cgaacctaca aaatgttctc ggctgaaagc gaagaaggag gaaacaaatg
    29761 aaaaagaaaa ttactattac tgcgatgagc ctgttaacgg ctcttttttt attgccaatt
    29821 aacggatttg cctatactat caacaatgaa tttaatttgg gcgcaaatga aggtagctca
    29881 caagtagcaa ataatcagta tattttactg catgaaacgg ctaatgaaac agcaacagga
    29941 cgcaatgaag cgcagtatat gcaacgttca tggactagcg cttatactgc ttatattgtg
    30001 ggagacggcg gaattgttta tcaagtcggt caacctggtt atgtacagta cggtgctggt
    30061 tcgtatgcta atgccaacag tcctgtgcag attgagttac aacacacaca tgataaagca
    30121 acgtttgaga aaaactacaa ggcatacgtt gaattggcta gagattcagc aatgaaatat
    30181 ggtattccat taacgttgga cactccttat aaccaaccgg gaatcaaatc gcatttatgg
    30241 gtaacacaaa acatctgggg cgatcataca gatccttacg gttatctttc tgaaatgggc
    30301 gtaagtaaag aaaaattagc atatgattta gctcatggat ttaccgatga aaatccaaca
    30361 acttctgaaa acaagcctgt cattgatcca acacgagctg gtgcagctaa tcctacgctg
    30421 acagatggaa caaatcacgc ccacattgat cagtttgggg aaatcgaaaa cgcaaacttg
    30481 catgtggctg gatggcacat tgctaactat aaatacgagt atattttcat tatggactac
    30541 aatactggga aagaattagc tcgagtaaga gctgatggaa tttatagacc agatgtaaac
    30601 caagcttata atacttctgg aaatgttggt tatcatgtat ctttcaatat gcgtaatttt
    30661 cctagtaaga aagtctatgt aatgatgcgg gcaacgaatg atccagaggg aaacactaaa
    30721 ggcggtgcgc aagatttcca tgacaaacgt tggtatttaa atattcctaa acgataaaaa
    30781 tagctcctcg ttgaggagca gtacataact atattgacaa ctataaaaat tattcgataa
    30841 aatagtgatg ttatcgcata tcttcactat cacccataaa tagtcacact ccaagctatg
    30901 cgataacagg tttgttgcca cacattctac tggttgattg tttatggctt tatgtggcaa
    30961 caaccagtac ccttagctca gttggtcaga gcagacggct cataaccgtc cggtcgtagg
    31021 ttcgagtcct acagggtaca ttaacgtagc catttgaatc gttctgtgtt agaatttttt
    31081 tgaagagtat tatacaagct aaagcttttc ttcattgcca ctcaaatgag tggctttttt
    31141 atgtatcctt ttatggatta atgaaaggat gtttcacata gttatacttc tgtatatttg
    31201 aaaagtttta ctttgatttt taaatagaaa gacatttggg ttaaattgtg agataataat
    31261 aaagaagagt ttaaagcgca ccccaaacca cttccccata agtgtgttac gctttaaact
    31321 cttttatatt tgaagccatt aaaaagcata ccatattttt gaaaaaaagt gagaaaaaag
    31381 gcttacaatt ggagtggtag ttaattagtg acttattttt gattttatag cactgatact
    31441 ataaaatata gatatcatca tattacacaa tcttaatact aacttaaaaa atatctcctt
    31501 tcacaagtat ggtgataaaa ttcgttccgg gctacctttt taggtagcct actttaatct
    31561 ttataccttt ctggatcaac gaaagtatac tttatatagt cataacgccg atgatcgcta
    31621 cgtgcgtccg gcacgtcagt catgaatcgg ctacactaga ctagacagaa aaaataaggt
    31681 gtgtagaata ataaaaaaca cactggagga tttttcatg
//
LOCUS       DACDPS010000025        31200 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.025, whole genome shotgun sequence.
ACCESSION   DACDPS010000025 DACDPS010000000
VERSION     DACDPS010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.025"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(149..580)
                     /locus_tag="IWJ07_001233"
     CDS             complement(149..580)
                     /locus_tag="IWJ07_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP9332823.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(598..918)
                     /locus_tag="IWJ07_001234"
     CDS             complement(598..918)
                     /locus_tag="IWJ07_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9332824.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            1217..1993
                     /locus_tag="IWJ07_001235"
     CDS             1217..1993
                     /locus_tag="IWJ07_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP9332825.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            2008..2211
                     /locus_tag="IWJ07_001236"
     CDS             2008..2211
                     /locus_tag="IWJ07_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9332826.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            2227..2565
                     /locus_tag="IWJ07_001237"
     CDS             2227..2565
                     /locus_tag="IWJ07_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAP9332827.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            2552..2731
                     /locus_tag="IWJ07_001238"
     CDS             2552..2731
                     /locus_tag="IWJ07_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332828.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(2774..3244)
                     /locus_tag="IWJ07_001239"
     CDS             complement(2774..3244)
                     /locus_tag="IWJ07_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332829.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            3331..4029
                     /locus_tag="IWJ07_001240"
     CDS             3331..4029
                     /locus_tag="IWJ07_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP9332830.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            4207..4548
                     /locus_tag="IWJ07_001241"
     CDS             4207..4548
                     /locus_tag="IWJ07_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332831.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            4541..5212
                     /locus_tag="IWJ07_001242"
     CDS             4541..5212
                     /locus_tag="IWJ07_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP9332832.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            5218..5904
                     /locus_tag="IWJ07_001243"
     CDS             5218..5904
                     /locus_tag="IWJ07_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332833.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            5907..6656
                     /locus_tag="IWJ07_001244"
     CDS             5907..6656
                     /locus_tag="IWJ07_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9332834.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            6668..6937
                     /locus_tag="IWJ07_001245"
     CDS             6668..6937
                     /locus_tag="IWJ07_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332835.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            7099..7401
                     /locus_tag="IWJ07_001246"
     CDS             7099..7401
                     /locus_tag="IWJ07_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332836.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            7398..7559
                     /locus_tag="IWJ07_001247"
     CDS             7398..7559
                     /locus_tag="IWJ07_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP9332837.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            7556..7861
                     /locus_tag="IWJ07_001248"
     CDS             7556..7861
                     /locus_tag="IWJ07_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332838.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            7861..8217
                     /locus_tag="IWJ07_001249"
     CDS             7861..8217
                     /locus_tag="IWJ07_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAP9332839.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            8177..8422
                     /locus_tag="IWJ07_001250"
     CDS             8177..8422
                     /locus_tag="IWJ07_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332840.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            8419..8838
                     /locus_tag="IWJ07_001251"
     CDS             8419..8838
                     /locus_tag="IWJ07_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAP9332841.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            8835..9392
                     /locus_tag="IWJ07_001252"
     CDS             8835..9392
                     /locus_tag="IWJ07_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAP9332842.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            9389..9685
                     /locus_tag="IWJ07_001253"
     CDS             9389..9685
                     /locus_tag="IWJ07_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332843.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            9762..10175
                     /locus_tag="IWJ07_001254"
     CDS             9762..10175
                     /locus_tag="IWJ07_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP9332844.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            complement(10633..10908)
                     /locus_tag="IWJ07_001255"
     CDS             complement(10633..10908)
                     /locus_tag="IWJ07_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332845.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            11362..11568
                     /locus_tag="IWJ07_001256"
     CDS             11362..11568
                     /locus_tag="IWJ07_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332846.1"
                     /translation="MLGQCFGNHARFDSLPATLNAYDGYDYRKKIVKKLYGATYGNVV
                     SVLSVHHQASVTVAEVEDAAVNGE"
     gene            11764..11931
                     /locus_tag="IWJ07_001257"
     CDS             11764..11931
                     /locus_tag="IWJ07_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP9332847.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            11957..12301
                     /locus_tag="IWJ07_001258"
     CDS             11957..12301
                     /locus_tag="IWJ07_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP9332848.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            12306..12587
                     /locus_tag="IWJ07_001259"
     CDS             12306..12587
                     /locus_tag="IWJ07_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332849.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            12690..13004
                     /locus_tag="IWJ07_001260"
     CDS             12690..13004
                     /locus_tag="IWJ07_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAP9332850.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            12982..14676
                     /locus_tag="IWJ07_001261"
     CDS             12982..14676
                     /locus_tag="IWJ07_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP9332851.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            14696..15874
                     /locus_tag="IWJ07_001262"
     CDS             14696..15874
                     /locus_tag="IWJ07_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP9332852.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            15837..16523
                     /locus_tag="IWJ07_001263"
     CDS             15837..16523
                     /locus_tag="IWJ07_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAP9332853.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            16523..17683
                     /locus_tag="IWJ07_001264"
     CDS             16523..17683
                     /locus_tag="IWJ07_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP9332854.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            17693..18568
                     /locus_tag="IWJ07_001265"
     CDS             17693..18568
                     /locus_tag="IWJ07_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332855.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            18565..18876
                     /locus_tag="IWJ07_001266"
     CDS             18565..18876
                     /locus_tag="IWJ07_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332856.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            18866..19219
                     /locus_tag="IWJ07_001267"
     CDS             18866..19219
                     /locus_tag="IWJ07_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP9332857.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            19209..19610
                     /locus_tag="IWJ07_001268"
     CDS             19209..19610
                     /locus_tag="IWJ07_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332858.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            19603..20007
                     /locus_tag="IWJ07_001269"
     CDS             19603..20007
                     /locus_tag="IWJ07_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332859.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            20019..20624
                     /locus_tag="IWJ07_001270"
     CDS             20019..20624
                     /locus_tag="IWJ07_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP9332860.1"
                     /translation="MAKTTTVTTFDNISIKRISFNFKNATNAISTDCNGQLDGETEMQ
                     RIVKKCGSTEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDERIKSGIYSYGPDSVG
                     EDFSLAAEIVDDFEEKNKLIGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFGK
                     FYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            20644..21006
                     /locus_tag="IWJ07_001271"
     CDS             20644..21006
                     /locus_tag="IWJ07_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332861.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            21009..21191
                     /locus_tag="IWJ07_001272"
     CDS             21009..21191
                     /locus_tag="IWJ07_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332862.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            21208..24639
                     /locus_tag="IWJ07_001273"
     CDS             21208..24639
                     /locus_tag="IWJ07_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP9332863.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDFIVAIGGLTAAFGILLPAILAVSYLFGPMML
                     IIGGIIAVIAGVIVAIKNWGAITDWFSGLWKKFTDWLGDTWESIKDGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISGIFDNLNISIPHIPLPEFIMEGSF
                     NPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGVGEAGPEAVLPLKRSVLQEIGDRILS
                     STSVSSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKIQNDNASAWR"
     gene            24690..25427
                     /locus_tag="IWJ07_001274"
     CDS             24690..25427
                     /locus_tag="IWJ07_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP9332864.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            25437..27728
                     /locus_tag="IWJ07_001275"
     CDS             25437..27728
                     /locus_tag="IWJ07_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332865.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVDGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDNGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            27752..29878
                     /locus_tag="IWJ07_001276"
     CDS             27752..29878
                     /locus_tag="IWJ07_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332866.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGDYDYSENPNLMPYITEPWVG
                     PLLGNGHTVKDSVKRVITHTKTRTANSGDILSLGLGIPCTAEANNRYLITTLRPSTTY
                     TLSVTMSVGSDWTGETNTIGVRLRYLNEQGGIELPINALIPANVERDKMVTHTFTGIT
                     KDNVTSITNCYVEIFSLNSEYKGTVSVSYDVKLKAHYPNLLDGPYWLGKVPLGENIAD
                     PTVVFPHKTSEYMVYGRRNTENYIADQTYTISMKATKLTVQSFAVYIAAGRVKVGDMK
                     PTEGLANTWELTFTVTKQHIDSGVTNYLEIYQYPSATKGAVQIEWLKLEKGNTRTPNI
                     SEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINS
                     QLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKL
                     YREGKTVSIYFVALNGKRSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSI
                     GADGAIKWRRNSSYGSDYTFAITYTI"
     gene            30041..30487
                     /locus_tag="IWJ07_001277"
     CDS             30041..30487
                     /locus_tag="IWJ07_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332867.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            30489..30626
                     /locus_tag="IWJ07_001278"
     CDS             30489..30626
                     /locus_tag="IWJ07_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP9332868.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            30664..30957
                     /locus_tag="IWJ07_001279"
     CDS             30664..30957
                     /locus_tag="IWJ07_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332869.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            30954..31178
                     /locus_tag="IWJ07_001280"
     CDS             30954..31178
                     /locus_tag="IWJ07_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP9332870.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
BASE COUNT        11160 a         4730 c         6481 g         8829 t
ORIGIN      
        1 ttaaaatctc tttttccatt ttttcttcct catttctgtg atatgatttt ttttgtagaa
       61 gctcatatat gaggaaagag cccgtgttgc agcacgggct tttttaatgt tttgctgaaa
      121 tcggtttttt taataattct tgatgtattc atacatattg ccttgcgtaa gaatattttt
      181 tcttaaaata gcattggcag acagcataac aagtgtatct gagcttattc tgaatagaat
      241 aatactccat aaatttttcg agattaaact gagattcatc agtcagttcg ttctcaatat
      301 agatatttaa taaaattaga atagctattt tatctgcttc tgtttcaaat tttgagtgaa
      361 aagttgtaga agtatcgtac aaaactgaaa attcaaaatg agaggcgctg aaatgtgcaa
      421 gttcatgaga taaatgaaag gcttctgcag tttcaccgta tagattttca ttcaaaaaaa
      481 tgattctggg ttttggatag tagaaaccag gctctttcat ctccatataa actactttca
      541 aattgtattc gctcaacatt tctttcaatt tcaaatacat acaaaccatc actccaacta
      601 ttcattttcc tctaaagctt tagcaattgc aatcgcttta cgcattgtct ccttagatat
      661 ttcttttcca tcaaaagaaa aaacagtatc gtcttctgat aaatccacat gtttaggggt
      721 ttctctttct tctctaccta gaaggtagtc tacagagaca tcgaaatagt cagcaatccg
      781 acttaattca tctgaattag gagtattatt tttccacttt gctagataac cgttagaata
      841 tccaaaatta atttctaact gccgtataga tatctttttc ctcttcgcca agtcttttat
      901 tatttcatag gtattcatcg ataaaacaac ctttctaaat gtttacaaaa aaagtttaga
      961 aaaatacgca gaaataattt gactaattct gagtaatgag ctatactata tcttgtaaac
     1021 agatttaaca actaaaaaga caacaaaaaa cactattgat atgtaaatgc agaccgccaa
     1081 gaaagcttta aaatcaatgt ttttatgtct tatttaatta tgctcttagt atagagtatt
     1141 actcagatac tgtcaattga atttagaaaa aagttgttaa atttgtttac gaatataaaa
     1201 gaaaggaatg agaaatatga acacaccgca aatttttaat ttcgaacaaa acgaagttcg
     1261 gacaatttta gtaaatgatg aaccatattt tgtaggcaaa gacgttgcga gtgttttggg
     1321 ttattcaaac actaaagatg ctttgtcgcg gcatgtagat ttggaagata agatggggtc
     1381 gcgaatcacg acctcaggtc aatcaagaga gatgacaatc atcaacgaat ctggtttgta
     1441 cagtttaatc ttaaaatcaa aacttccctc tgccaaaaaa tttaaacgtt gggtaacaag
     1501 tgaagtgttg ccagcaatta gaaaacatgg aggttatcta actccagaaa aagtagaaga
     1561 agctttgctt aatccagata caatcattca attagcaact caactaaaag aagaaagaac
     1621 tggaagatta atcgcagaac aaaagattgc agagtacgaa cctaaaatct cctatttaga
     1681 tagcatatta tcttctacag attcagtaac aattagtcag attgcagcag attatgggat
     1741 gtctccacaa cagatgaata aattacttca taaactaggt attcagaaaa aagtcggtaa
     1801 ccaatggtta ttgtgcaaaa aacacatgaa ccaaggatac acaaaatctc atacaactga
     1861 gatcccgaaa gccgatggtg gcactaaaat tgtaatgaat accaaatgga cacagaaagg
     1921 gcgtctattt atctacgaat tactaaaaaa agaaggatat taccctcaaa tggatttaga
     1981 ggaaattggt tagaaaggag ttttagtatg actgacattg cagaaatcac tcaacgagat
     2041 agagaaaaaa tcaaagaata tgtcgaaagt tcaaagttct taacttacac catgcttgct
     2101 gaaagatttg gaattagcaa aagctactta tctttaattt taaacggtaa aaagacttct
     2161 gcagaagcaa acagaattat agattcgatt atcactatgt acgaattgta aagggaggaa
     2221 cagctaatgc aatatctaga agcaaaaatc ccaattccag aaggctatgt aattatctcc
     2281 caagtggatt atgaggagtt aaaaaaagct gatgatactg gtagatggat gacgttgcca
     2341 gaagtgctag aacggattaa cagaaaatat gattggttta cttctagagt tttaaagaac
     2401 ccaagatata gaaatattat cgatatcgag aaaaacaaaa atggatttgt ctattatcca
     2461 gttgaaggaa gagacacata tctattttta agaagtaaaa cacttgaatt tttagaaaca
     2521 aatttttcgg aaatcttaag gaggcaagcg gatgggaaaa tttaatagag cgctagtgtt
     2581 cagcgcaccg ctaatcatct acgctttagg actttgggga agcaggcaag cgttgatagg
     2641 aacgattgtt tacatggttt ggatttttat ggggcttgat gaagctgaag ctgagtacag
     2701 agcgaaaaag ccaaccgagg gggctgacta aaaatgaata gaaaagagaa actagaatgt
     2761 atattactat tactcagttt aattctttca ctaatttctc tattgggaag tttgtatttt
     2821 tgatatcaac aattttgtgg atttgggttc tatttagagt aaatatcatt tttgtttggt
     2881 gattaaggag tttattaaaa ttaattttcc ctacttcaaa tgcaaggtaa caagatatag
     2941 cacttttagg aggaatattt agcggtacag cttcagagta tattctatta tcatcgactg
     3001 atcctccttt agcaattaat ttcttaaatg gagtagctga ccatctgcgg tttaagttag
     3061 cggatgaaag ttctaaatta actaacatag ctggttctgt tgaatagtta gaaatgatta
     3121 cttttgtata aatcatatct gcagcgaagt aagaagcatt aagttcaact tctagttgag
     3181 ggcgcattcc ttttattttt atccctgtta aaaacgtact caatataaaa cttattattg
     3241 acatccattg aaaaattgtt aagtgaaaaa aattcaaaat aaacaccacc agtttttaac
     3301 taaattatac caaaaaggag agaagaaata atgcaagaat tagtaatttt gaaaaataaa
     3361 gaagctgtga ctacgagctt acaagtggca gaaagcttcg agaaaaaaca tcaacatgtt
     3421 ttaagagata ttgatgcact aaaaaaagat gtgtccaatt ttggacagat gtttgtagaa
     3481 ggtaatgaac cagattcata tggcagaaat cgacgagttt tcttcattag tagagatgga
     3541 tttttcttgc tggctatggg gtttacagga aagaaagcta tctacttcaa acaaaaatac
     3601 attgaagcat tcaacgaaat ggaagatgtt attcgcaaga atactgttcc tcaaacaatt
     3661 gaagatatga tgatctatca attagaagaa atgaaagatg ttaaaaaaga tgtttccatg
     3721 cttaaagata ctatgcgaat tagcggacaa caagagtttg aaattaagca aaaaggaaat
     3781 atgaaagtta tgggagttct agggggaaaa gaaagccgag cttatgaaga aatcagcaaa
     3841 aaagtattct caaaattttg gtctgaattt aaacgtacct tttcaatccc aagatatggc
     3901 gagttacctc gtaagagatt cgatgatgct gtttcattta ttgaaatgtg gttgccagaa
     3961 actgcgatcc gtatggaaat tgatcaactg aacagacaac agagactttt tggtgatgac
     4021 aatgaataga gctgaagcgc taagaatagg gacggcaatt gctaattgct ggtggaaata
     4081 ctataaacca atcatcctaa gccaacaaca tattgacaag caaaaagcat ggcaacaaat
     4141 aaaaaagtga ctccgccggc aagcaaagag tcacaaagaa aacacatcat aaggagattt
     4201 tagcatatgg aaaaagaact ttccactcta gatcaatatt tgattgatcc tgattggggc
     4261 aagccgaaaa ttgaggaaac aagtggtcga aaaatcagac gaaatctttt gacgaatgaa
     4321 gaactagctt gggatcaaga tgatttaggc aaccatgtaa ctatttggga tcatgtttat
     4381 cttatccatc tatcgaagca ttcgaataaa cctgaatata tttacgtcat cgaagatggc
     4441 ttgattgatg cgctagaaga gtacgacaga gataacttga ttgatatctc ttattacgga
     4501 ccaggtaaga aatacattgc tgaaatggag gcagaatttg atgagtgaaa tcaaagggac
     4561 aacgaacttt gaaaaacttt ttagtcgtaa gttaaataaa attctcaaga aaaaaggaaa
     4621 ttttgattat ttatcttggg ctcacgcgtg ggagattatg aaaaagaatg atccacaggc
     4681 aacggtaact attaatgagt acaaacacta cagggttgtt tctggaactc atcaagactt
     4741 tcttgttgag gaatataaac cttttcttat ggatgaaact gggacttatg tatctgtctc
     4801 agtaacggtt aaaggacaca cggaaaccga attatttcct gttttagatt atcgaaacca
     4861 accagttgtt aaaccaaatg caatgcaaat caataactca ttgaagcgat gctttgtgaa
     4921 agcattggct ctacacggac tgggattata tgtatttcaa ggggaagata ttccaacacc
     4981 acctagaatc gatacaaaga aattaaacat gctagagacg attctagaag ctttcaatga
     5041 gcagatgggt aaagatatga ccaaaacctt aatcgaatat gttaatgagc agacagataa
     5101 attagggctc ttagctgata acgttgaaac tattgaacag ttaagctatg agcaatgtgc
     5161 cttgatggag cgagcaatag cagctaagag aaaagaatta gataagaagt gatatgagtg
     5221 tttaaaccat taatcgattc atattcagcg gttctgaaaa agttcaaagg aaaagacata
     5281 agcgcaacca tcaatgagga agtgaacatt gatcgactaa agacgatgta tgacggctac
     5341 gatggtgatc gagtcattga aattcgtttt attgatccta gacgtttcac cgtacagcaa
     5401 cgaaacttca tctatgcgct gataggcgat atttttatcg atacaggcat gccaacggac
     5461 ttctggaagg aattcttcta ctttcgtttt gaaggtgtca cagggcgcaa aataagcctg
     5521 aaagacgaat cgaatacaac tgtgagtgat gccaatgtct tagcaaatat catcttagat
     5581 ttcatctttg aacatcatat tcctttcaaa gaaggctatg agattttacc tgcgaatcaa
     5641 gaatattact tctacaaatg catcacaaaa agagtctgct gcatctgtgg caaaacagga
     5701 gctgacatcg atcactttga caaagcgcta ggaagacgaa agcgcaaaga agttgatcat
     5761 gcagagtaca catttgcagc actctgcaga atccatcaca cagagaagca caaaataggt
     5821 gtgattaatt tcaaaaataa atatcaaatc aaagggatca aattaaacca gaaaacaatc
     5881 aaaaagttaa atattggagg gtaaaaatga cagaacatcg aagttattat gcgattatac
     5941 cagccaacgt aaggtacgac aaaagactta aaccaaatac taagttgtta tacggagaga
     6001 taacggcctt gtgtaatgaa agaggctttt gttgggcagg caatgagtac tttgcagatt
     6061 tatatggtgt gaataaagag accatatcgc gatgggtaag tgatttgatt aagtttggat
     6121 acttgaatcg ggaaatcatt tacaaagagg gtaccaatca aataatcaat aggtacctac
     6181 gaattaatca ataccctatt gacgaaaaac gcaatacccc tattgacgaa aaagtcaaag
     6241 ataataatac atctattaat aatacattta ataatacaaa agaatatata agagagttac
     6301 cgccttcgaa aaaatcgaag gctaagcccg tccgtcataa atacggagag tataaaaatg
     6361 ttcttttgtc agatgagcag atggagaaac tcaaaacaga attccctaat gattaccaag
     6421 agcgaatcga acgactgtca gagtattgtg aatcatctgg taagacttat aaaaactatt
     6481 tggcaactat tcgaagttgg gcaaggaaag aaaaaagtga acctaagaac gcaagcagtg
     6541 gatacaagcg cacaggaaga cgagagaagc ttcctgaatg ggcaatcgac caagaagcct
     6601 atctcaagaa aaaagcgcta gaacgagcta atagacaatc aaaagcacca ttctaagagg
     6661 tggaaaaatg aagatcgatt atctagaact aattaatgaa atagcgaatt ataaaaaggg
     6721 cgaggaatta gacgtcctga gagacgtata tgatcaactc gaagaagctg gaattgaagg
     6781 aattaagaat gatcgttcga gttggagtaa gctcagatac tatttcgcac tttatatcga
     6841 tacaacacaa ttaagaaatt tagcatatac caaattacta tttgttgatt gtgttaaagg
     6901 cttgcaaaaa catcttaatg aacttgagca ggtgtaatca gatggaccta aagacattta
     6961 cagcacagat tgaactaatg catcaagaag ctttaagaca aagtgtatcg tacgaagaca
     7021 agtggctcaa cacgttccat ggcggacgtg agagcgcact tgatcaagta ctcaaattac
     7081 tgaaaggaga atgtcgggat ggataagaaa gcggcaatgc agcgaattat cgaattgact
     7141 tattcagaag attggcaaaa tgacaaagaa gctgcttcag aagtgatgag gcttggaaga
     7201 gcgatgtggg cagacaagag caacaagcca agaccacgaa aaatcgcaat ttggcacggt
     7261 gacaaacttc tagtgatagg gacagctgaa cagttagcaa gtctcacagg cttgcacgag
     7321 aaaatcgtga ggaaaagagc aaggtgtggc tacacagacg ctaagaagag aacgtttaga
     7381 tacgtggagg aatcatcatg acaacagaag aagtgattca aatgcgtatt cgaagccttc
     7441 agcgtgagat tgacgatctg gaacgaacaa aggcagtgat ggtcaatgaa acggcgagaa
     7501 aggcaatcga tttgcacata gagaatttaa gaagggaaat ccatcgattg gaggaatgag
     7561 cgtggataag aaagcagcaa tgaaaagaat tgctgaatta accaagtcag aatcttggca
     7621 agaagacaaa gaaatagttg cagaagtcca aaagctcggt aaatcaatgt ggactgaaaa
     7681 gcccaaacgg agaacgccga gaaaaattgc aatctggcat gatgaccgaa ttttagtaac
     7741 aggtactgct gaacagttat ctgaaattac tggattaagc aaaaacatta tctgggatag
     7801 agctaggagc ttatggattg attcaaaagg acgacagttt aggtatgtgg aggagagata
     7861 atggatctca ttacacaata cagtgacatc atcctcaaga aaatcatgat gaagattcag
     7921 aaagataaaa aatcaaaaga acgagctgaa ttagttaagt tagaaatggc tgaaacagga
     7981 gcaggagtgc gaagtagcag gcattggaaa gcagcagcaa acattgaatt ttattacaac
     8041 gaaattcaaa aagggttcga tcagatgcgt gagctggatc ggcaaacaaa ttggagcaag
     8101 aaacttcatc aagatcgttt caaatttgta gagaagtata aagaaatatt agaagagtat
     8161 ttgaggagga cagcaaatga taaaaaaact cgttcaattc agcatggatt tatatgatat
     8221 cgaatcagga gcaacactat ctgtggaatc ggaccatcta atcataaatt ttggtggaaa
     8281 gcgccagatt attttgtggg tagttgatga tgtactgttt ccagaaattg ttcatgattt
     8341 cgaagaatca aaagcggttg agtttgaaat agtgaaaaaa gtaatggaat tgattgaaaa
     8401 atacgaggag gacagcgaat gataccgaag tttagagcgt gggataaacg aaagaacgta
     8461 atgagagatg tagccgtctt gcattttact aaaaacggca aaacaaactt tattgaatat
     8521 tggataaatc ctaccgaatt gaaatcatat cacgtgcgaa acatcgacct catgcaatcc
     8581 acaggaatga aagataagaa tggtgtggat atatttgaag gggatatagt attagtcagc
     8641 gtgcgaaatg gcttcgatta cttagataat aaagtctgta ttgtcaaaaa ttcaatagat
     8701 tattccggat tagtttgtgc cactgttgat gaagacttag agtatcaaat ttttaacaca
     8761 gagctgtttg aagaatacac gtacgaagtc accggaaata tatacgagaa tagcgagtta
     8821 ttggaggaac agcgatgaat aaacaggaag caatcaaaaa gttagaaagt attaaagcga
     8881 taggaaatga tgcaatagct gcttgctata acgagagtat aaattcaggt attacgttaa
     8941 tgaaaaaaat agacgaaccg cagaaaccag ttattccaca actcgtggcc ggttggcttg
     9001 agaaatctac ggaccctttt acaaaagctg aaaaaatagc gtatttaatc aaatctaaag
     9061 atggtgattc atattatttc tgtgattggt ttgtacgaga tggcatagtg acgcaagagc
     9121 aaggagaaga attactcgct tgggcaacaa gacaatcata tgaaacacta ttgagcctat
     9181 acaacggcta cgaggttgag aaagagccgt tatgggcaat aaagaatgcc gatggaaact
     9241 atcttactaa atgtgcttta tggggaaaag atggagtaaa ttatagtttt gaatgcaatc
     9301 catctcatcg attgcttttc actgataaag caacagcgga tgctgcagca ttgttggtga
     9361 atggaacagt ggaagaggtg gtagaaagat gaaactaaaa gacggatttt acgctagcag
     9421 tcacggtatc ggcggtttaa tgctagatat gccgacaaag aaccctaaaa cacgcaagaa
     9481 atcaaaattc aaagtcggtg acatggtccg ctgtgaagca gaagagttcg tttatccgtt
     9541 cagaggatac gttaaaaaga tactgtcaaa ctcagcaatc attcgtattg aaaatacaat
     9601 gaagtgtgat cgttcgactg caaaacacaa gcattattta gctgtagcga gattagttga
     9661 catggaagta atcaagagca aataaaaaaa gccggatcgc tccgactgat tcaataaatc
     9721 caacacattt attatatcac ataaaggagc ggtttgactt gatgcaattg ttacgagagg
     9781 tagatttcaa acagacaaga tgtaatgcga gagatgtgct gaagaacttt cggcgtttgg
     9841 agcggatggc aggtcgctct ttgatagata ttaagtcgcc gattataacg gatatgccga
     9901 aggcaccgaa gcatggcaat aaggcagaag acgcgattat tcagatgatg gatatagaaa
     9961 cggagagaga tgcgattcta gcggctttga tggctcttag tctgattagt cgtcagatac
    10021 tctactacag cttctgtgtg ccagatagct tctcaaacta cagaattagc cgtgaagtgg
    10081 gttattcaga aagaagtata caacggatga agtcggaagc tctaatagag tttgcagaag
    10141 catataaaca cggaaaaata attgcttata aataatttgg cggttttttg gcggaatgat
    10201 ggcggttttt agacatttac cagtgttatt atggtagtgt cgaaagatta ggaaacagga
    10261 tcgacaaaat aaacgaaggg aggcaatctc cctcatcgtt ttaaacttct tcttagatag
    10321 acagcaacta attatgaaga aaaggatgtg aattcaactc ctcctaaatt cttcatcggt
    10381 tgctgtttat tttgattaga cagcagcact cacaaaacta aacatgttct tgtcgtcttt
    10441 caactgctgc tgtctattaa tttatgtatt ggagggaaga aaaatgaaat tatcagtaga
    10501 aggcactcca gaagaaatac aagaattgct ccaagctatt ggtagtagca aggagcaaaa
    10561 ggtaagtgtt tctaattcag atattgacgg tatttgcaat tgtttaaaca gagaaaaaat
    10621 atctaatcct tactaagaga aaatatgttc gttaatgaat ttccaatttt cctctgtagc
    10681 tctaccttga taattttttt gcagttcaca gatgaataag gaatcgttat tatcaagtac
    10741 tgtatttaat ttttcacaca tttgatttgg attcaaatta gttttaacta accaaaaaga
    10801 tttttgtagc tttatatatg ccccaaattc ttttattatt tcaaatactt tatcatactt
    10861 ttgccctggg ttattaagat cataactaat catgtagggt ttatacatgt tgtcttgcac
    10921 atccttgttt tatatttcag cggaccactc gctgataaat aaaattatac gcttagtatt
    10981 tattttcaca atattaattt atcgtaataa cttttgagta aatagaacaa aaaacctgca
    11041 ctagtttccg ctagtacagg cagtgactat atcggttgat aatctagcat atattaaaaa
    11101 taattgcaag aaaagaaatt tatagtggtt tataccaaat tatcaaagca atgttatttt
    11161 gttgctgtct attgtttttt aattattcac acgataacta aaggtgggtg aagagaaatg
    11221 attccattaa taatttcaat ttttgcgctc tgtcttaatg tctatatgat tggatttaaa
    11281 aatgggcaaa ataaaaaata gtagcagcca agaataattt tatagtgtca ctgtggcgga
    11341 aagggtagac gctaagcatg tgtgctaggt caatgcttcg gcaaccatgc aaggttcgat
    11401 tccttgccag cgacattaaa tgcctatgac ggttacgact accgaaaaaa gatcgttaag
    11461 aagctatacg gtgctacgta cggcaatgta gtaagtgtgc tatctgtaca ccaccaagct
    11521 tcggtcactg tggcggaagt agaagacgca gcggtaaatg gcgagtagcc tcgtgagagc
    11581 ctggtaagtt ctcgtgagtg gtgcaatccc actccagcga ctttaagcaa ccgaggcatc
    11641 ggcggtttaa aaatataggg gtgcgcaatt tcgtacgcgt tttgtgcatc gtgcaagtta
    11701 ctattacata ttagatcact ctttgagtgg tctttttatt tttgcacaaa ggaggaaaca
    11761 acaatgtata gaccacaata cttagaacag aagtatgaag taatcactgt tcataaaggt
    11821 aatggcgaaa aagtatatga gtatagaaga ccaataaaga gcgatacata taaacgaaag
    11881 gaaaacaatg aagttattcc attgtatggc aaaagaatag ctaagcatta aataagattg
    11941 cgaaaggaga cggaacatga ccgaggaatt ctatagatgg ctattacagt tgacaagaga
    12001 agatcgtttg gttaagttct atcagtctcc taaatggcgc aggcttagag agaaagcgat
    12061 gaaacgagat cactatgaat gccaagagtg tagaagacta ggtaagtatc atagagtaga
    12121 gaacgttcat catataaagg aagtcaagga tagacctgac ttagctttag atttagataa
    12181 tcttatttgt ttatgtgttg aacatcataa tgaagttcat ggcagatatc ttacagcatt
    12241 agataaacaa gagaagaaga tagaaagctt tgctaacttc gatgcaagtg aaaggtggta
    12301 agtgcatgat catcaatgat aatggcagag agtatgatac agaaaagatt gaagagtatt
    12361 catcttatac tcagggatta attaaacgtt tgatatacgt tcgctatgta ggtattaggg
    12421 atctgttatc agataactgt tgtagtaaat acaaagtgaa tcaagtaaga gaagcgttga
    12481 ataaagataa taacgtcgaa agaataaaaa atgtttttgg atatagtatt gaagagatta
    12541 attattacat tgacttcgct gaagctttca ttccgatggt gagataaccc ccccttaaaa
    12601 taaatcgcaa attttttggg ggtgatgaaa cggagggggc tgtcaggaaa agagattttt
    12661 tcgaacttta tcatgaaagg agggctaaaa tgtttaaaaa cgaattgtct caaaatcgct
    12721 acagagaaaa attacgccgc tctttaataa gccaattgga aagtcagaaa acaaatattg
    12781 agccattctt agataatgtt gatcgttata tcagtttatg ggaaacggcg atatcactgg
    12841 aagaagatat atccgagaac ggcattagac tggagaatgg taaaaagaat gaatcagtag
    12901 cgttgcttgt ttctgtcaac aaacaaatgg gattgatgtt ggataaactt gccattactc
    12961 ctgaattggt aggtgaagca aatgaatcaa ttcctgagtt ataagcatat tgaaaattgg
    13021 ttcaaagcta tagaagaagg cactatcaag gtatgcaaag agcaattatt gctaaaaaat
    13081 tatctagaag aaagagtctt tactagagaa gatatttact tcgataagca gatggtagag
    13141 gattcaatca atataccagc acaatacttt ccattcgaat taattccgtg ggaaaaattt
    13201 ctacaatgtt ttatttatgg tgttcgatgg aaaaaagata aaacactagt gttcaataga
    13261 tatctttcat taatgggacg tggtaatggt aaaactggtt ttgcttcttg gaacaacttc
    13321 tttctactaa ccgctaaaca cggtattaaa aattatgata ttgatatcta tgccaataat
    13381 gaaagccaag caaagactag ttttgatgat gtatttaaag taattaaaga tcatcctgat
    13441 ttagataaaa aagtatttaa agctacgaag gaagttattc aaaatatcgc tacaaacagc
    13501 aaacttcgtt ataacacggc aaatgctaga acaaaagatg ggaagcgacc aggtgcaaac
    13561 cgctttgatg aaattcacga aaatgaagat tattcaatga taaatgtggc tacttctggt
    13621 ggtggtaaaa ttcgagatta tagagaattt tatgatacaa ctaatggtca tgttcgtggt
    13681 ggtccgcttg atgacattat agaagaatca aaaatgattc tttctggaga acttggaatt
    13741 gacaaggatg gagcagaatt ttctagtttg tttccattta tttgtcgctt ggataacgat
    13801 aatgaagttg atgatcccga catgtgggaa aaagcttgtc caactattaa ttacaatgca
    13861 gatttaaaac ggaaaatgtt tcaagaatac tctcaaatgc aacgtaatgc tggtttaaga
    13921 cttacgttca tgaccaaacg aatgaacaga cctatggaag atacacgatt tgctgttgct
    13981 tcatatgatg atgttctgca tacgaaagaa aaagaatttc ctgaaaaaat ggatgaagtg
    14041 ataggaacag tcgattttgc tgatagacga gattttgcca gcgttgggtt gctaggaaaa
    14101 tacgataaag atgtttattt tacacaacat acttttatcc acgaatcagc ccttcgatta
    14161 caaaacatca aacgagaggt tatagatatt tctatagatc aaggaaaatc acagatcgtt
    14221 catggaaaaa atatagaagc tgattatatt gtaggttggt ttcttgaaat gagtaataaa
    14281 tattatatta aaaaaatcgc tatggatatg taccgtgcaa aaatattgaa gcccgcttta
    14341 gaagaagcag gttttactgt ggaaattgtt cgaagcggat ctgttacaca tggtatgtta
    14401 aaagatctgg ttgatgacct ttttattaat caacgtttat tttttggtga cgatgcgatt
    14461 atgcgttggt attgcatgaa tgtatatgaa gagcatattt ctaatggaaa tatacgctat
    14521 gaaaaaatag aacctgaaac tagaaaaacg gatggctttt tttcattcct tcatggtttg
    14581 aattttttag atgatattta tgattctgct cctgtaacag tcacaaatag ctcagtagaa
    14641 aatacaggaa ctggatttac tcctctagta ttctaacttg aaaggaggtg agaaagtggg
    14701 gatttttcaa aaggcggtag gatacttcac aaaaaaagca acggttcctt tagaagaata
    14761 cttttgtaaa ttgcaagttg attttgtgta tcgaaaattt gcgattgaaa cttgtattga
    14821 tttgattgca aatgcgatga gtaaagcgga attcaagtca tatgaagatg gaaaaaataa
    14881 aaagaatgat ctttactata ggctgaatgt agctcctaat aagaaaaata atgcaacaga
    14941 atttagaaaa aaactgatca ggagattaat attctacaat gaagtattga tcgtttctcc
    15001 gtctaataat tctagcgaaa tatttattgc ggatagttgg gatgtcacag aatatgcatt
    15061 gaaagatgat gtgttttctc aagtgcaaat taacaacata gtccttgata gagaatttct
    15121 agaaagtgat gttatctata taaaatacgc agatcaacaa attaggcaac tagtcgatgc
    15181 gtattatcaa gcgtatggga aactcatttc tagtgccatg aatgtttaca agcgctctaa
    15241 cgctcgtaga tacgtactga aagggaattt attccgaccg caagacaata caacacaaga
    15301 tcaaatcaat aaaatgatga catcacaatt taaggctttt atggaagctg ataatgcagg
    15361 tgcggtattt caattacaaa atgagtacac attagaagat ttcagcggaa acttccaaag
    15421 caattcaaga gatataaaaa acttaataga cgacatcttt gagatgacag cagcagcgtt
    15481 tcacgttccg aaaaacctac taaagggaga catgagtggg ttatcggatc aagtggacgc
    15541 ttttttaatg ttcgaaatca taccgattgc tgaacttatt caggatgcgt ttaacgctag
    15601 tctctatgaa gtagaagaat acttgtcagg gaattttgta cgtgtggata caactatgat
    15661 caagattact agcttcaaag atttggttga cgctattgat gtaggcatta gaaatggagt
    15721 atttacaatc aacgaaggaa gagaacgcgt tggaaatgat cgctctgata aggcgatggc
    15781 agatgaaata tttataacta aaaacaatca acaagtatcg aaaggaggtg aggcgaatga
    15841 cgacaatgaa aacatttcta gcagtaaaga atgaaggcgc agtaccgcaa atttttattc
    15901 agggatttat tggttctagt tggttctttg aagggaatac tgacaaggga atcaaaaata
    15961 ttttggatag tctaggtgat caagaagaaa ttgaagtagt aattaattca aacggtggag
    16021 acgtatttca agggattgct attgggaact tacttaagtc aaataaagca aaagttaacg
    16081 ttgtgattaa cggcttagcc gctagtgctg cttcaattat cgcaatggct ggcgatacta
    16141 taaaaatcta caacaatgca caattgatga ttcaccgcgc ttccacatac ggagaaggaa
    16201 atgtcgatga cttccgtacg attgctgacc aactggaatc aattgataaa tcggtaaagg
    16261 cttcatataa aacacgattc aatggcacag atgaagcatt gcaagaactt cttgaaaaag
    16321 aatcgtttat ggatgcagaa acagctttga gttatggatt ggtcgatgaa attatcgatg
    16381 cagaaaatag ctcaggtact gaagctaaaa aagaacaaag cgttgaagaa attttgaatg
    16441 acgttaaaga aaaaagagca gaaaaaattg ctgcatttac agcagcatta aataaaacat
    16501 ttggacaagg agatgtaaaa taatgacagt taaaaattta aaaggtgtaa cagctgcaag
    16561 cgaccaattg atgaaagctt ttaaagatgg taacgaagaa tcttttagcg cagctatggt
    16621 aagcttatct aaggaaattc aggataaaat tttagaagaa gcaacagcaa aaaatcaaga
    16681 tcaattagta ttaatgaacc gtggtcagcg tgtattaact acacaagaaa caaaattcta
    16741 taacgaagta gtgaaaaacg aaggttttgc aggggtcgaa gaattagtgc cagctactgt
    16801 atttgaacgt gtatttgaag atttagaaca atctcatcca ctattgcaaa aaattacttt
    16861 tgttaacaca actggtgtaa cagaatggat tgtgtcacgt ggagtcaatc cagcatggtg
    16921 gggtaaactt tgcgaagctg ttaaaaaagt tttagataat ggctttgatg taattaacat
    16981 gaagcagttc aagctatcag gttatattcc tgtatgtaag gcaatgcttg atttaggtcc
    17041 agtatggtta gatcgttatg tccgtactgt tttagtagaa tcattgagaa ttgcattaga
    17101 acaagcaatt gttgatggta ctggtaaaga tatgccagtc ggaatgatgc gtgacatgag
    17161 caaacaaact agcggagaat atgctgaaaa aaaagcagaa cctattacag ctttagatgc
    17221 tgtaactatg ggcggtttga tggcgcgact atcaaaattc aatatcgaag gtgtgaatga
    17281 tccgatttat cgtaatgtga atccttctga tgtggtccta attgtgaatc caactgatta
    17341 ctggtctaaa gtattcccag ctaagactgt actaactgct aatggagaat acgtacaagt
    17401 attgccagta ccagtttcag atttgcagtc aacggctgtg ccagaaggaa aagcagttat
    17461 tggggtagcc tcagattact tcatgggtgt aggatctaca ctaaaaattg aagcttcaga
    17521 tgaataccat tttgttgaag acgaacgcat ttatctagct aaacaatatg caaacggtca
    17581 acctaaacgt aacgatagtt tcattgtatt agatattagc gctttgggaa ctactactac
    17641 aactacaaaa ccaacaacca caacaactac aacacaagcg taggtgatca gaatgaagta
    17701 tattctttgt cagccggcaa tcaatcggtt taaatgggag cttgaagttt gtttaactaa
    17761 tctgaagaaa ctaggaatca aagatatcgt attgcttttc agcagacacg atgatcagat
    17821 tcctattttt tttgagaagg aatatggtgt tgaagttcat gtgtacgatg atctgcggga
    17881 cgacaaagag tatattcctt cgattaaacc atatttatgg tggaaatatt tagaagaaga
    17941 tcattcgcgt gaggacgacc gatatttcta tatcgattcg gatgtcattt tcaataaaag
    18001 aattaatttg cgcaaattgc cttctaaaga tgatgtttgg tattgtagcg actgctgtag
    18061 ttatctaagt cttgattata ttagaagctg tgaaaacgga gaaaatattc taaaagatat
    18121 ggcaaacatt gtaaatgtta cagtagaatc tttggaaact ataaacacta attcaggagg
    18181 cgcacagtgg gttattaacc gccctaaagc gaattattgg aaaaaggttt atctggattc
    18241 taatcggcta tatcgctacc ttagagggca aaaaacaaat atacaaatct ggacagccga
    18301 gatgtgggca cagctttgga acatgatgta tttcaatatt ggtcctaaag ttcacgagga
    18361 attagacttt tgttttgcta ctgatccaat agaaaaagtt aaagaagtaa aaatcttaca
    18421 caatgctgga gtaacaacaa acgatgaaga tttatttttc aaagggagat acgtgacttc
    18481 tacgcctttt gatgaagatt tatcatttgt aaacaagaaa aaatgctctt acgcatatgt
    18541 taaagcaatt aaggcggtgg ttagatgacg cctgaacaag tgactgaaga attgctaata
    18601 gctgtgaagg ataatattta cgttacctgg aacgaagaag atgagtcaat taaaaagatg
    18661 atagctaaaa atgctgttta tcttcaaagt aaagtgagta caacactttc tttttctcct
    18721 gaaagcttag aatacggatt gctaatcgaa agatgtagat acgactggaa tcgtgcttta
    18781 gatgagtttg aacaaaattt cgctagtgag ttattaggtt tcattcaaca ttatgcgcta
    18841 caagaatata ttgcaggtga tgtgaatggc gaataatcgt agactcgaag aaacattcaa
    18901 cgatggttgg ttaaagattt tgacgcaaac taccaaaaga aatgaactag gaaaaaagat
    18961 tggtgtagaa gatacagaaa tcacttcttt aaaatttaga aatctttcca tgagagatag
    19021 tgatataaca gctatggatg cgatgggatc gaaattaact aagaaagtaa agactccatt
    19081 tcatccaatc gccaagaaat ttaataaaga tcaatatttt atcgtaatcg atagtatgcg
    19141 ttacaacgtt atctatgccg attacgataa tttttatatc tatttttatc ttgaaagtgt
    19201 gggtgaatat ggtgattgat aattctaaag aaaaagaacg tttaaataag caaatttctg
    19261 ctatcaaaac ttccttagaa gagcattttg gcctcaaact ctttcaagac tccgtaggcg
    19321 aggatgagct acctgatgat tttaattact tcattctcga aacaggagaa atagaaatga
    19381 tcactgagcc aaaatatagc gtgggtcaaa atctatatct aactttctat tcagaaaata
    19441 gagaagattt aacaggagat tcactagata ttatttcatt gattcaaaat cgttcgattc
    19501 gttttcagag aatggatccc aatcatttaa aactagagaa ccaagatcgc tatatcgatc
    19561 aattggtatt tacgtttaga cgattattga agagtgattg tcatggctaa aaatagttgg
    19621 gagctaaaaa taaatggaca tgatgaactt cttgtgcgga tggaacgcta ttcaagcgag
    19681 agcgaacgac tgattaacga agcattgaaa tcaaaaggtt cggctattgc agtggatagg
    19741 attacagaaa aaattcctgt ttctgaagca gatttaagaa gagggcacca acacgcaaaa
    19801 aatagtcgtc cacttaagac tcaatacatt aatttgggtt tcatcattag acctacaaga
    19861 aaatttgagt atttaaaata tcctgatttg gggataggta cttctaaaag aaatcagccg
    19921 gacgaattta tgagaagagg attaggtctt gcacttgatc caattacaga acttctgatt
    19981 cgtcaattcg ataaattaaa taaataggag gaacaacaat ggctaaaaca acaacagtaa
    20041 caacttttga caatataagt attaaaagaa tttcttttaa ttttaagaat gcaacaaatg
    20101 caatttcaac tgattgtaat ggacaattag atggcgaaac agaaatgcag agaattgtaa
    20161 aaaaatgcgg ttcaacagaa gtaaaatcga aatctaaacc aatcaatatg acggtaacaa
    20221 ttactgcaca tgtaccgatg gaagtttatc gacgcttcaa tgggttgaaa caagatgaac
    20281 gtattaaatc gggaatttac tcttatggtc ctgattccgt aggggaagat ttctctcttg
    20341 ctgcagagat cgtggatgat ttcgaagaaa aaaataagct gattggtatg ttagcatgca
    20401 cttcgaatac aggattaaca ttctctattg aaaatggtgc ggatgaagta gctgcgttag
    20461 aactagaaac aaaagttatg caagatgaat ttggtaaatt ttatcatgaa gcaattgttg
    20521 cagaacttga agaagactta acagatcaat ggatgacgaa tctatctgct gatgtgatta
    20581 aaaaaacttc agttgtgaca actacggcca ctcaatcaca gtaaaaaaaa cggaggtagc
    20641 gaaatgaacg aagattactc aaaaattgaa ctaaacgatg gaacaatttt gaatttagaa
    20701 cctaaactga atatcaagaa attattgatg atcaatagag attttaacac agacgagttt
    20761 gcaaaaatga ctgtgggaaa aggatccatg gatatttctg ttattcaagg tgcaaaggct
    20821 gtgtatattg cttaccgcca agcgaacatg actgattata tttcattcga tgaatttatc
    20881 gataaatggg attttgatat ggctactgcc agctatattt atcaattgat gatgttcaaa
    20941 caagcacgcg atgcttatca aaaagaattt gaaaaagcaa ataaggaaaa aaagcttcaa
    21001 aagtaaaaat gccaaagctc ttagttgaaa cgtgggtcga tgtctattcg atgttgaccg
    21061 acgttttttc tatgccttca gatttggttt taagcgatat ctgtttagat gacattttgc
    21121 aaatggctta caacaagagt gcttatgaag gatggaaaaa ctatgcaata aaccaatccc
    21181 agaaaaacta aagaaaggag gtaaaaaatg gctaaaaaga gaacagaagc agaagtaaca
    21241 ttcatagcta acgatgacgg attgaaatct acgttaaaag aaatcagtgc tgaattaact
    21301 aaaaatagag cagaattaaa actagaacaa gctcaattac aacagactgg ttctgaatca
    21361 gacaagttag gaagtaaatt atcttcttta gaaaagcagt atgaattaca aagtcaaaaa
    21421 gttgaagtaa ctagccaacg tttagccaat gccaaaaaat attatggaga aaattccacc
    21481 gaagttcaga aacttgagag agaactgatt aaccaacaaa cagcgcaaca acgtttgtca
    21541 aacgaaattg ataaaacgag taatgcacta gctcaagcaa aaggcgaaat acagacgtac
    21601 gagtctacaa tgcaacagtt ggatagtgaa caaaaaaatg ttcaagctag tgcttctctg
    21661 attgaatccg aatacaaaaa atggcaagca actgctggtc aatcagcttc tgaatccgag
    21721 aaattagcga aagcccaaga atatgtttct caacaatctg aaaatgcaga gaaaacgata
    21781 gatatcctga gacgacagtt agaagctaca cagtctgagt ttggcgctac atccacagaa
    21841 gcaatgcaga tggaggcgaa gcttaatgat gctgaacgtg aatttgaaga gttaggacaa
    21901 gctgctaaaa atgtagatac aactaacttg gacgatatcg gaagcaaaat agatatgaat
    21961 aatctaatgg aagcttctga cgttttaagc gacattggcg ataagcttac agaattaggg
    22021 aaacaagcag tggactctgc taatagtgta ggtagttccc agagtaaaat acaagctaat
    22081 tttggtttga ctaaacaaga ggctgaagaa ttaacgaatg tagccagaga catttattat
    22141 aaaggttttg gagaatcgtt agatcagtcc acagatgcat tgattttggt aaagcgtaat
    22201 ttaggcgatt taaataatca agatttacaa aatatcacgg aacaagctat ggtcctagaa
    22261 aacaccatgg gcgctgatat ggatgaaacg ttacgtggtg taaatggctt aatggtcaat
    22321 ttcggcttga gcgctcaaga tgcaatggat ttaatggttt cgggtactca aaacggttta
    22381 gataaaacgc acgaattagg cgacaatatg gcagaatata gccaattatg gagtcaaatg
    22441 ggatattcag ctgatgaaac gttcggaatg cttcaaaatg gtttagatgc gggtgcttat
    22501 aaccttgata aagtcaatga cttagttaag gaaatgggaa tatcgttaac agatggtcga
    22561 tttgagcaaa acatggatat gtttagtgaa agtactagaa aagcttttga agagtggaaa
    22621 aatggcggag gaacacaaaa agacgttatt aattccatga ttcaagattt tagcaatatg
    22681 gatggtcaat acgaccaatt aaataaagct tctacaattt ggtctgcact tggcgaagat
    22741 aacgcgatga aagttgtcca atctttgact gatgttaacc atacatttga tgatgttagt
    22801 ggatctgcac aaaaaatgaa tgaagattct actactccgt tacaagaatt aaacggaaaa
    22861 atagctgaat taaaggattc attagctcct ataggcaaca caatcataga tgcactcgaa
    22921 ccagtaattg attttctagg aaagatggct gatgcgttta ataatcttcc acaaccagta
    22981 caggatttca ttgttgctat aggtggtctt acagcagcat ttggcatact gttaccggca
    23041 atactagccg tgtcatattt atttggtccg atgatgctta taattggagg aattatagct
    23101 gttatagcag gcgttattgt ggcaattaag aactggggtg caattactga ctggtttagt
    23161 ggtttatgga aaaaatttac tgattggttg ggtgatactt gggaaagtat aaaagacgga
    23221 gcctcatcag tttgggatgg ggttaaagaa acctggtctg gatttgtaga ttgggttcaa
    23281 gatatttggc aaggagtttc tgattggttt ggagagctat ggagcggatt agttgaagga
    23341 gcttccaaca tctggcaagg agtccaagaa acttggcaaa cattcgttga ttgggtttca
    23401 aatatttgga acggagtcaa agaagtatgg tcgattattt gggcagacat tgtaggaatt
    23461 gttcaaatac catggacatt aataacgtca ttgattcaag ctggtattaa tattatcgtg
    23521 ggtatttttg atgtagctgg acagttattg ggcgcagctt ggcaagctgt ttggacacct
    23581 atttctgatt tccttaaaaa tacttgggat actatgacac aatggataag tatcgcttgg
    23641 aatgggattg taactacatt ccatactata tttgatccag tagtggcatg gtggaatggt
    23701 atatggacag ctattagtac tacggcttca aatatttgga attcaattag tgcaacagct
    23761 tctagtattt ggaacagtat caagaataca atcactagct tggtacaagc agctgctaca
    23821 gtaattcaaa atatttggtc aactgtatct agttggttag gtggaatttg gaattcaatc
    23881 agctctacag catcaaatat ctggaatagc gtgactagta gtataagcaa tgctataaac
    23941 gcagctaaaa gtgccattca aagtgtttgg aatagtatat cttcgtggat tagcggaatt
    24001 tggaacggta tcaaaaatac tgctttgaat ctttggaatg gaattacaag cactattagc
    24061 tctaaagtaa acgatggaaa aaatgcaatt tcaagcggtt ggtccaatct aacaggtatt
    24121 gtttccgaca tattcaataa tgttaaaagt acaattgcta acatatggga aggcatcaaa
    24181 aagactgtta gcgctccaat tgattggatt agagacaaaa tcagtggcat ttttgataat
    24241 ttgaatattt ctataccaca tattccgtta ccggaattta tcatggaagg cagctttaac
    24301 ccgctaaaag gtcaaatccc ccatttgcgt gtaaaatggc atgctaaagg aggtatcttt
    24361 actaaaccaa ctttactagg tggaatgaac ggtgtcggtg aagcaggacc tgaagcagtt
    24421 ttacctttga aaagatctgt tttgcaagaa attggtgatc gtatcttgag tagcacatca
    24481 gtttcatcta gggcacaaac gattcaacct gtgaataact acgaattcaa tttcacaatt
    24541 gatggtaacg cagatgaggt tactatgaag caaacaactc aacaaatcat tgatagcatt
    24601 acaaaaattc aaaatgataa tgcttcggca tggcgttaaa caggagagta tttctcctgt
    24661 ttttttagta ttaaaaagga tgtgaaaaaa tgactgattg tatacattct ataatcgatg
    24721 gatttcctga ttatttgcat aaattggctt tagctgaaag accaaccata ccttctccaa
    24781 aaagacagag agttgaaact tctgttttag gtaggttagg tggcttagta caagattact
    24841 cgtttgaaga tatgtcgttt acattgcact ataactattt agaggatgtg gaagaccatc
    24901 aagcgttcaa gcaatcgttt tatatcatgc gtcattggtt aaactatgca aagaaattag
    24961 aattctctga cgatcccaac gtctattacg ttatccagac tatcgatatt ggggatgcag
    25021 aaaacgatat tgttgaatgg ggagagttcg atgtaaatat tactgcgaaa ccattcgcaa
    25081 gagttcaaga agatgtacct ataaccgtag ataaaccaca gtcatttaac ttgctgaata
    25141 atagtttaga agaaagtttt ccaaagatta tcatcactcc ttcagctact tcatgccagt
    25201 tcatcttaaa tgattatgtg tttagtttcg aaggcttagt agcaggaact gatgtagtca
    25261 ttgatagtga tttgatgctt tgctacgaag agcaatcgga cggagatatt ttagatcggt
    25321 ccaacaaaat gaagaccatg caatatccga cattgcaagt ggatatcaat cattttaatt
    25381 gcactggttt gagcaaaata caaatttatc gtaacgggtt aaggtaggtg aaatagatga
    25441 tcgataattt aataactatt tacgataaaa atgacgcgaa taatttagct gaacatttat
    25501 atgatacgca aggtttaggc gctttgtcag actggttaac agctactgtt agcaataaac
    25561 taaacggagc cgagatattt cagggtactt attcaataaa cggaactaat gcagatttga
    25621 ttgtagacgg acgtattatt cagtgttatg tagatgaaaa tcgagcaaaa cagcgtctac
    25681 ggatttatta tgcaaaaact tctgtaatag gcaatacgat agaagtaaaa gctgaaccta
    25741 ttttcaatga tataagaaaa tcggtgttga ataaatatga cagcggaaca gaaaagatca
    25801 ctgctactca ggcatggcaa aacgcaaaag ttttagcgaa accagctatc ccttcgcagt
    25861 tttctttctc gtcattagta gatacgcttg ctaatgtgaa gatagaaaag gcgaattttt
    25921 tagaattctt tggtggaaaa gagggatcta ttctagatcg atttcatggt gagtttctaa
    25981 aagataataa cacattacgt catgaaaaaa gtctaggtac ggatcataaa atcaaagcga
    26041 tttatactaa aaacttaact ggtcttgact tagagattga tgctcaaagt gttttagttg
    26101 gagtttatcc attcattagc agctcttcag aaggagaaga cgagatcact ctaccagaag
    26161 aagttatttt cacggattac gtggatgatt atcctgctgg atatgtttct tttgttgatt
    26221 ttaaagacaa agcgactgat gtagccacat taagggaagc tgctaaagac tggttgaaaa
    26281 caaacataga taaacaaaaa ccacaagtga gtggttcgat tgaattagta ccattgaggc
    26341 atcaaagagg ctatgaaaaa tttgttgatc tagaaaaagt ttcgatgggt gacggagtag
    26401 atgtgtatca tccacagtta aaagtgaata tgtcagcgag aattgtggaa tatacgttta
    26461 atgttctaac caattcatac gataaattag ttgtaggaaa cgtcaaaaca aacttcttag
    26521 aaaacacaga gaataatgtc agcaatttga ttaatgatgc cattgatcaa ttgaaaaacg
    26581 gtggcgaaat cagtgattta ctcaatgata ttgtagatca tcaaactgat atgattactg
    26641 gtcaaaatgg tggttatgtt ttattagatc ctaaagaagc gcctagtcgt attttgatta
    26701 tggacacacc agataagaat accgcaagga atgttttaca aatcaacaat gctggtattg
    26761 gtttttctaa aactggcatt aatggaacat atgaaacggc atggacgtta gatggcggat
    26821 tcaatgcctc gtttattacg gctggtgaaa tagtaggaat tactattaga ggtactacat
    26881 taattagtga tggtgctgat tatagaacaa gtattgctaa tggcaaaatg acttggtact
    26941 caaaaaaagt taacaaagat attatggagc tagaagcacg tgattatgta agtgctgatg
    27001 ccggtattgt atcatacacc atgaaaactg gtggtggttt catgattaga aatccacagg
    27061 gtaacttggt ttttagtacg tgggataatg gtaataacag accgtttcta tcttttggtg
    27121 cgcccaattt caggtatagc aatgctagtt atgtaacttc tggcgacggt agttctttaa
    27181 gcattaatgg tagtgcgggt aactcatggg aatttaaggt agctggtagg actatgaaat
    27241 ttactagtga tggtatgcta acgttaccag gttgtttttt tggttcatgg gaagatggga
    27301 aacttgctag gtttgaacaa tcaacggtac aagtatataa agattttact gttagaggta
    27361 ctaaaaactc aactgtacca acagaacatt atggacaacg actattgaac gcttatgaaa
    27421 ctccagaata ttatttcgct gattatgggg aagccgttac aggtgacaat ggtaaagttc
    27481 gtgttgatat tgaccccatg tttgctgaaa cagtaaatct aagtcggtat atgacacatg
    27541 tgacacctac agaactagtt ttgtgtgctg ttactcatga agatattgac catttcatca
    27601 ttgaaactag taagccaaac gtattagtta gatggaattt agtggcacac cgtctagggt
    27661 atgaagatat tagattaaaa gaggatacag catatgatag cacagtgctt gaccaaaaac
    27721 gtttttaaaa cgaagacaag gaggtatata aatggctagc agtttatata atttggcttt
    27781 agatttcagc aaagaattaa actacaccaa agctattatg gctcgtcaag gtgataaagg
    27841 gattacggtg acggttaaac cgtttctaaa tggcttgcag atggatacga gtggcggaac
    27901 atttacttta aaaggaacaa caccatctaa ccgttacgta gataatgttg caactagtgt
    27961 aactagtgaa gaagtcacgt tttctcttga tggcacattt atgagtgaag caggatatta
    28021 taaacactgc tacgtagaat atagaaaaga caatcaaatt ttaacaacgc aagatatcat
    28081 ttttttctca ctaggagtgt ctgacatttc gcaaggccaa gccgatgaat atgtttcgca
    28141 attagaagag ttgattcgaa agtacaacga aacttttgat gcttttatgg ctgaaatcaa
    28201 aggtagagtg gatagcttaa atcaacagat tactgattta actggtcaag ctaaaacgct
    28261 acaagacaag ttagatgctc tgaaagaaga aatttctaag ttaggtaact tacaagtgat
    28321 gtacagtaac agcatcgact tcggggacta tgattattcg gagaacccga atttaatgcc
    28381 atatattaca gaaccttggg ttggaccttt gttaggtaat ggacatacag ttaaagatag
    28441 tgttaaaagg gttataactc atactaaaac tagaacagct aattccggcg acatattatc
    28501 gcttggctta ggtattccgt gtacagcgga agctaataat aggtatctta taaccacttt
    28561 acgaccatct acgacataca cattaagcgt tactatgagt gtcggttcag attggactgg
    28621 agagaccaat actatagggg ttaggcttag atatttaaat gaacaaggtg gtatagaact
    28681 gcccattaat gcattaatac cagcaaatgt agaacgcgat aaaatggtca ctcatacatt
    28741 tactggtata acaaaagata atgtaactag cataacaaac tgttatgttg aaatattctc
    28801 acttaatagt gaatataaag gaacggttag tgtcagctat gacgttaagt taaaagcaca
    28861 ctaccctaac ttactagatg gcccttactg gttaggtaaa gttcctttag gcgagaatat
    28921 tgctgaccct acagttgtct ttccacacaa aactagtgaa tatatggttt atggtagacg
    28981 caatacagaa aactatatcg ctgaccaaac atataccata tcaatgaaag caactaaact
    29041 aacagttcaa tcgtttgccg tgtatattgc tgctggccgc gttaaagtag gagacatgaa
    29101 gcctactgag gggttagcta atacatggga gttaactttt actgtaacta agcaacatat
    29161 agatagtggg gtaactaatt atctggaaat ataccaatac ccgtcagcta caaaaggagc
    29221 tgttcaaatt gagtggctca aactagaaaa aggcaatact cgaaccccga atattagtga
    29281 gtataaatat cgtggtactg gtatgcgtga ttcaaacaat ccaaaagatt atgtttggga
    29341 tctagcacca gaatatgtcg aagataattt ggccacagat attaaaattt ctgaaattac
    29401 tggtaaagca aacaattata ccgatgggaa agtatcggag attaattcgc agttgactgc
    29461 ttcaattaat gaagtagaca ccacagctaa ggatgctcaa acaaaagcga atgctaatgc
    29521 gactgctata gatgaattag acaataagat cgatgaacgc attaatgata cagctactac
    29581 cacattaaca gttacaaacg ggaataccgg atcagcaaag ctttatcgtg aaggaaaaac
    29641 agtttctata tattttgtgg ctttaaacgg aaaacgcagt ggtggaaatg attcaacgat
    29701 actaacaatt ccagaaggct atcggccacc aattagtttt gagcaactgg ttggctcgat
    29761 agaccgttct actttgaaca gtgctcagtt atctattggt gcagatggag ccattaaatg
    29821 gcgaagaaac tcaagttatg gatcggatta tacctttgca attacttaca cgatttagaa
    29881 aagcgtgaat cgatatgaag gcagcatata gaccaattga accttacgga ttcgagcaaa
    29941 tcattgtgaa tgatgaagaa catttaccgg aagaatgcac agaagtcgaa ccaccgattc
    30001 caaattggaa accgaaattc aattactggg agggaaataa atgaaaaaca tttggaaata
    30061 tggacgtact ggcggagagt acgcaggaaa agtattggac gacatgcttg tatccgttcc
    30121 ttacacagat cagccaccgc ttgaaggaat tcgtgctgat ggcgaaccgt taacaatcgc
    30181 tgatcaaatg tttgatccta aattgaatca atggattatt ttagcgaacg cactagatca
    30241 caacgattta aacaatctca aagcaatgta tgagtcgtta gaaaatgaga acggcgattt
    30301 aaaacagatc aatgccaaac tcatgctaag cgatgtagca attaaacagg aaaatactgc
    30361 attgaaagaa aaagcggata gtttagcaca aatcaattca aaaatgatgc ttgcttcgtt
    30421 acaaaatagc aaagacattt cagaaattaa agagcaacta aatccagctt caaagggagg
    30481 tgagtagtat gtttagtttt agcgatgtga aaatgatgta tgattggggc tgtttcacta
    30541 acgaacaagt aatggttttc gttccgttgt gcattactga agaaaaagca gataaaatca
    30601 ttagcaaaga agagagcgca tcttaattga tgtgcttttt attttgattc aaggagttgt
    30661 cacatgatta atttagggga atggggagcg atagcaggat caataaccgc tatcgtttct
    30721 ttgattttat tagtaataaa accaattact gcatctttct cgaagattac tgagactctt
    30781 tcaaaagtaa gtcacaattt agatttgctg actaaagatt tagaatcgag caaatcagat
    30841 cgattgatga ttcatgaaga actaaagaaa cacgatgaaa gattagatac acatgcagaa
    30901 aaattggtag aacacacgca acaaattaaa actttattta gggagagaag aaaatgaata
    30961 ataaaacgtt cgaagtacta aaatggttcg cactggtaat tattcccgca ctagctactt
    31021 tcgtggggtt agttggtaaa gcgctcaatt ggcagtacac agatatctgt gttgtcatca
    31081 ttactggttt tggcgcgttt ttagggagtg tgttgggtgt atcaaatcga acctacaaaa
    31141 tgttctcggc tgaaagcgaa gaaggaggaa acaaatgaaa aagaaaatta ctattactgc
//
LOCUS       DACDPS010000026        31087 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.026, whole genome shotgun sequence.
ACCESSION   DACDPS010000026 DACDPS010000000
VERSION     DACDPS010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.026"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(162..1934)
                     /locus_tag="IWJ07_001281"
     CDS             complement(162..1934)
                     /locus_tag="IWJ07_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9332871.1"
                     /translation="MRMQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINM
                     VPAFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     VQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1888..3642)
                     /locus_tag="IWJ07_001282"
     CDS             complement(1888..3642)
                     /locus_tag="IWJ07_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9332872.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3752..4321)
                     /locus_tag="IWJ07_001283"
     CDS             complement(3752..4321)
                     /locus_tag="IWJ07_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAP9332873.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(4339..5763)
                     /locus_tag="IWJ07_001284"
     CDS             complement(4339..5763)
                     /locus_tag="IWJ07_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9332874.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDIN
                     FDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQF
                     FTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSK
                     TNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGG
                     IKKDIKLRVEDSTLIEQIFNDITK"
     gene            complement(5765..6433)
                     /locus_tag="IWJ07_001285"
     CDS             complement(5765..6433)
                     /locus_tag="IWJ07_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP9332875.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(6563..7147)
                     /locus_tag="IWJ07_001286"
     CDS             complement(6563..7147)
                     /locus_tag="IWJ07_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9332876.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(7478..7711)
                     /locus_tag="IWJ07_001287"
     CDS             complement(7478..7711)
                     /locus_tag="IWJ07_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAP9332877.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(7726..8676)
                     /locus_tag="IWJ07_001288"
     CDS             complement(7726..8676)
                     /locus_tag="IWJ07_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP9332878.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(8673..9515)
                     /locus_tag="IWJ07_001289"
     CDS             complement(8673..9515)
                     /locus_tag="IWJ07_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP9332879.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(9517..10236)
                     /locus_tag="IWJ07_001290"
     CDS             complement(9517..10236)
                     /locus_tag="IWJ07_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP9332880.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            10928..11086
                     /locus_tag="IWJ07_001291"
     CDS             10928..11086
                     /locus_tag="IWJ07_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9332881.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            11158..12384
                     /locus_tag="IWJ07_001292"
     CDS             11158..12384
                     /locus_tag="IWJ07_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9332882.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(12472..12864)
                     /gene="rpsI"
                     /locus_tag="IWJ07_001293"
     CDS             complement(12472..12864)
                     /gene="rpsI"
                     /locus_tag="IWJ07_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP9332883.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(12880..13323)
                     /gene="rplM"
                     /locus_tag="IWJ07_001294"
     CDS             complement(12880..13323)
                     /gene="rplM"
                     /locus_tag="IWJ07_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAP9332884.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(13842..14309)
                     /locus_tag="IWJ07_001295"
     CDS             complement(13842..14309)
                     /locus_tag="IWJ07_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAP9332885.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(14429..15967)
                     /locus_tag="IWJ07_001296"
     CDS             complement(14429..15967)
                     /locus_tag="IWJ07_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAP9332886.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            16047..16733
                     /locus_tag="IWJ07_001297"
     CDS             16047..16733
                     /locus_tag="IWJ07_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAP9332887.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(16934..17461)
                     /gene="msrA"
                     /locus_tag="IWJ07_001298"
     CDS             complement(16934..17461)
                     /gene="msrA"
                     /locus_tag="IWJ07_001298"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP9332888.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(17545..18222)
                     /gene="pgmB"
                     /locus_tag="IWJ07_001299"
     CDS             complement(17545..18222)
                     /gene="pgmB"
                     /locus_tag="IWJ07_001299"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP9332889.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(18219..19349)
                     /locus_tag="IWJ07_001300"
     CDS             complement(18219..19349)
                     /locus_tag="IWJ07_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP9332890.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(19349..21532)
                     /locus_tag="IWJ07_001301"
     CDS             complement(19349..21532)
                     /locus_tag="IWJ07_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP9332891.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(21529..22554)
                     /locus_tag="IWJ07_001302"
     CDS             complement(21529..22554)
                     /locus_tag="IWJ07_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9332892.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(22599..23426)
                     /locus_tag="IWJ07_001303"
     CDS             complement(22599..23426)
                     /locus_tag="IWJ07_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP9332893.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(23423..24490)
                     /locus_tag="IWJ07_001304"
     CDS             complement(23423..24490)
                     /locus_tag="IWJ07_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAP9332894.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(24516..25352)
                     /locus_tag="IWJ07_001305"
     CDS             complement(24516..25352)
                     /locus_tag="IWJ07_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9332895.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(25349..26218)
                     /locus_tag="IWJ07_001306"
     CDS             complement(25349..26218)
                     /locus_tag="IWJ07_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9332896.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(26288..27571)
                     /locus_tag="IWJ07_001307"
     CDS             complement(26288..27571)
                     /locus_tag="IWJ07_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP9332897.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(27585..29270)
                     /locus_tag="IWJ07_001308"
     CDS             complement(27585..29270)
                     /locus_tag="IWJ07_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAP9332898.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            29545..30570
                     /locus_tag="IWJ07_001309"
     CDS             29545..30570
                     /locus_tag="IWJ07_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9332899.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
BASE COUNT         9491 a         6212 c         5061 g        10323 t
ORIGIN      
        1 attattcctt atattataag aatagacatt aagtgaacta attcaatcat actgattctt
       61 tcgtgtattc aaaattagat gctgtttaat aaaattattc tttttgaaaa agaattataa
      121 ctatatccca aaaattgtag gttaatttaa agcctatttc atcatcgtga ttgagcttct
      181 ttttcataga cgaatttctc agatagttta gttttcttta tgagattttt gtatatttct
      241 gaccttcgat acaactcatt atgagtacct tgatcaacta gttttccttg atccatcact
      301 acaattttat ttaccttttc aatagacttc aagcgatgcg atataattag tactgtctta
      361 tcttgtatta aacgatttaa actttcctga atacgttttt cattttccgc atctaaactg
      421 gcagtaattt catctaaaat taaaattggc gcatttttca aaaaagcccg tgctatggat
      481 aggcgttgtc gctctcctcc agaaagtgaa cttccgtttt ctccgaccaa tgtatgataa
      541 ccgtcaggta gtttttcaat aaagtcttcg cagttagcta aacgagctgc ttttttcact
      601 tcctcatctg acgcttgact atttccaata cgtatatttt ccaaaatact tgtattaaac
      661 aaagttacct cttgaaaaac aatagcgatt ttgctaaaca aagaagctgg agatacacgt
      721 ttaatatcat acccatcaat taaaatacag ccttcatcat aatcatacag tctagacact
      781 aatttcaaga tactagtttt cccggaacca ctagcaccta ctaacgcagt cacttctcct
      841 tgtttagccg taaaagagat atgatctaaa attggggtat tgttatcata tgagaaagag
      901 acatctcgca actcaatgtc aaaagatttc agaggactat ctgatccctc ttggatactg
      961 gtttccttca tagctctaat tcgctgtatt ttaggagcta aataaaatat ttcaagcact
     1021 gcttctttca tagaatcgaa ggaatccttt atttttattg cggctagtaa gtaaccaacg
     1081 acgtagagta tagtcacttc acccgttaac agtaagtgaa cacctactag aatgactact
     1141 gctaaactaa catatgagaa aatagaggat aaggccatga ctgaaaaagt acttaattct
     1201 acctttaaat gaatcctttc actctcttcc atttttttga aaagtctgtc ttgaactttt
     1261 ttagatagat taaagctatt aatctcttgc tgcaattcta tagtttcttg aaattcttcc
     1321 gagttttctc tcaaagtatc gtaatattta gtattggctt tcgtttggga tttctttgat
     1381 aacaagatta agacaaaact aaataacgtt ggcaaaataa cagctaatcc cattttgaca
     1441 ttaccaacca aaagcatcac tgaaataaaa gggaaaaaca gagccatacc gccggcttta
     1501 ggtattgcat gactcatcgc atgctcaata ccttcaacgt cagacatgat agtttgagat
     1561 aaatctgata aattatgttt tgaaaaatag gataatggta aatttgacaa atcgtctgca
     1621 atttgcactc ttaaatgcgc actttcttta taagtactat tatataagtt ttcatactcc
     1681 ctatctaaca accaatacat gaaaagcaaa gtaacaaacg atacggcaaa ataaagccaa
     1741 cggggctttg tattttctaa aactaattcg tctatcagca tcattataat gaatgctggt
     1801 accatattaa tgcagtatac aaaaaaacta gaaatagaag ctttagttaa agcttctgcg
     1861 ccctgatcac ttaaagcaaa tttgttttta taaaaacttt tcattgtcaa gcctccaatc
     1921 atttgcattc tcataattag tgaccaattg tttatacact tgatgtttag ccattaattc
     1981 attatgattt ccacgttcta ctattttccc ttctgacaag acaagaattt catctacaga
     2041 tttaatgctg gataatcgat gagctatcat aataactgtt ttttctttca tcagatgttg
     2101 aaaagccttt tgtagttcat actcattatc tggatcaata gatgcactgg cttcatccat
     2161 aataacaatc tttgcatctt ttaaaattgc tctggcaata gctatacgtt gtttttctcc
     2221 tcctgacaga taaactcctt tactaccaat taaggtatgc tctctttcgg gaaatttttc
     2281 taaaatcgat tcgcaaccgg ctaatcttag cgcgtcaaat actttacttg ctggcgcttg
     2341 aggatttgct atagaaacgt tatcgtaaat actctgattg aatagtttcg tatcttgaaa
     2401 gacaaaagca attgtgttaa taattgtgtc ttttgaatag tgatttaaag gcttaccacc
     2461 aattttaatc tgtcccttat ccacattgta gaaacctgaa attagtttag ctatcgtact
     2521 ctttccgctt cctgattttc ctactaatgc ataaattctt ttttgttcta aaactaagtt
     2581 gaaattttct aaaacacact cttctttgta tgaaaagctg acattttcaa atgcaatgtc
     2641 acaattttta atctgtgtct cgttcccata ttttagttgg tcttgcttca tttctaaata
     2701 aagggagtct agtgtttcga ctgcatatct tgcttcaaac aagtgcatac ttacatacat
     2761 tattctcatg aaagaaacaa aaataattcc acttaaaaaa aacaacatca acaagtcaac
     2821 agctaatatc ttgggagtgg taagagaagt tataaaaaag gttataggaa ctatcaaaat
     2881 gcaaactatg ccaaaaaata gccattgaaa aagaacaaaa ggtgttttcc cactcaaaga
     2941 atacttatat gcaaacttag cataatcgtt aattgcttta ttcaaggctt taaatgatgt
     3001 tactttagca ccaaatatct ttataacttg aattccgcgg atatattcta ccgtttcaga
     3061 gcttaacaca tctaacgatt cttgatattt ttgaataaaa tttgtattct ctgtcatttt
     3121 ctttaacaag aatacaccaa ttactaacat ggcgattaga accaatccaa ttttaaagtt
     3181 aatgaaaaag cctaaggcaa ctgataaaac aggtgttact atggcttgag cactatctgg
     3241 aatcaagtga gcaattgcca tatgtgtttt agccgcgttg tcatcaattg tctttcgaat
     3301 atatcccgaa gaatttaaat caaaaaaacg aaaacttgct tctgttaaac cgctaattcc
     3361 cttttttctc aaattagtct ctaagcgaaa cccaaggata tgagaaaata agccagaaaa
     3421 aatataaaat aaagctccta aagttaacca agcaacagtt tgaaccgcat aactcttagc
     3481 taaatattca tctcctacaa taattagaga ctgaagaaat ttgtaaatac tgtaatatcc
     3541 atagactgtt aatacgacag aaaaatcaga aaataaagtg gctaaccatc cgtagtattt
     3601 cattttaggg acataactaa ataatttttt gtatactttc attttttcct cccccgcatt
     3661 caatttcttc tcttattcgt ttacaactgt taatgctttc cgtaatagcg taaattagag
     3721 atgtacaaac agataaaagg taaactacgt tctatttttt gtattttttt aagacttttc
     3781 ttccaatcaa tacaccaatc atagataaac ttgctacaac taacactgaa atgattcccc
     3841 atatacctac cgcatagtgg atcattgcat ccattgttgc agcagattgt cctttatcta
     3901 tccaatcttg acgaaaacga tctgtaaaga accatatagg aatccactgt ccggcagcac
     3961 tgcctaattg cattagagcc caagacgcaa cttgaggaat tgctcgatca tatccaaata
     4021 cagatagaat aatatcagct aagatcgcca tactaatcga actgataaac caaggtaagt
     4081 acgccccgcc cattaacgta aaaaataaca ataaaacaat tgtgtaaata gtaaaaattc
     4141 cttttctagg gtagttatag ctaatataaa caaatatgat tccagataat aatccaaaaa
     4201 tcccggggga tatgctgtga ccaaacgccc caccaatact actaaccatt attgaaaaaa
     4261 agtaaattac tagggaaata actgtagcta aaccaataaa aatataatct tttgttttca
     4321 tatatattct cctcaattct atttcgttat gtcattaaaa atctgttcta tcaaagttga
     4381 atcttctact ctaagtttta tatctttttt tattccacca ttttttaaat aaataattct
     4441 attgcatgtt ttataaagaa attccggatc atgagtagct ataactactg cacccttttc
     4501 ttttaagtct ttaataaggt cagcaatcct ttccatatta atataatcca acccacttgt
     4561 aggttcatca aatagaaata aattagtttt tgacaatgaa gctatagaaa ccaataatct
     4621 ttgtttttct ccaccggata aatcaaacgg taattgatct ttcattttat aaagttccat
     4681 tttttttaaa attttttctt gctgatcttt actaactatt tcattcccca gggctaactc
     4741 gctcctcact gattctgtga aaaattggta atccatttct tgcattacta aaaacggcga
     4801 tgacaaattt tttttatttt cgattatctt tcctttggta gggcgaataa tcttcatgag
     4861 tattcttagt aaggttgttt tccccacacc gtttttccct accaaaccaa taacatctcc
     4921 aactttgaca tcaaaattta tgtcttttag aatattttca taagtaattt cttcaacttt
     4981 taatacatct gcacttacct tttcgatata gggaacttca atcgaactat taaaaatgtc
     5041 aaatttcctt aatgcgttat tgttttcagt ttttaacaac tgaatagttg tagtttttac
     5101 agttctattt ttaacaaata gaacttgatc aattaattct ttcaaatagt aaaaacgatg
     5161 ttcagctata acaatagtaa atcctttatt ttttaaatca caaagtaagt cagcaagttt
     5221 cttagtcgct ttgaagtcca aatttgaaga cggttcatcc aatataatta atttagagtt
     5281 tagcgataaa ctacatccaa tagaaatttt ctgccgttca ccacttgata gcgaaaaaat
     5341 gtttttatct aatatatttg aaagatctaa taattcagct aattcattga ttcttttatc
     5401 catatgctct tttgtaaatc caaagttttc catcgaaaat actaactcac tcgttgtatt
     5461 tgttgtaaag aattgtcctc taggatcttg acttacgtag ccaatatttt ttgatatact
     5521 ttcttgtgtt aaggattgta gcgagttatt ttcaaataaa atttccccaa ccaattttcc
     5581 gtcataaagt cttggaataa gtccattcat taactttaat agtgtggatt tcccactccc
     5641 agaagaacca gtaatcaatg taagactgcc tttttttatt gaaaaattaa ggtcaacaag
     5701 cgcttgttcc ttaacatcct tatatgaaaa agaaacattg ttaaattcaa acaattcttt
     5761 cacctcaaat caaaatactt aagaaaaaaa tagtaacgga aaacaggatg atatagtcta
     5821 ataagctcat gtttgtttta tagtaagaac ttcttttcac aggagattct atcccctttg
     5881 ttattgcatt agcggtcatt tcttcagcaa tcatagttgc acgtataaga acgggaacta
     5941 acagaaattc caaatattgc agaggctgaa aaaaagacaa gcgaatgttt ctcattttta
     6001 aagattcttt aatcattctt atttcagaac gataagtcgg aaagaatctg atcactactg
     6061 taatacttaa cattattttt ttcggtaaat gtattttttc taaagaggat aataattcat
     6121 tgctagtata gacactaacc aacaaccatg agatcattac gaccggcaac attcgaaata
     6181 caatatataa ggatgtcgca aaaaatatag taaaaaactc tatcttgata gcatcaataa
     6241 ttttcattac taaaaacaga actgaataaa tcaagatgaa ataaatacat gtctttattt
     6301 tacgaaacat caatactaaa actgtagcaa taatgaaaca ccccacttga aatatatttc
     6361 cctttcctaa aaaaataatg accggaaaaa aaatatttaa agtaatcaag gttaacggat
     6421 tgatttttga cactctttca cctccaaaaa ataagatcgc actgttatct taagctgata
     6481 aaaatgcacc gttattcaga taacagtgcg atcttatcaa aaagcataac tgagaacaat
     6541 tctcaatgca agcttttttt aattaactca gtaattcaat ccacccagga acagaaaatt
     6601 gatacaatct tctggttaat agcatagtct tttcttttga atagctatgc ttcaatatat
     6661 catagaatgt agcgtagaat gagtaaatgt agaagtggat ttcttcttca ttaaagacta
     6721 aattttgatt gatcattccg atagtcttta acttatctag acaacagact gtctttttcg
     6781 tcacaaatga tgtaattttt tcaacaatat cttgatattt ggttccagct gattggaata
     6841 aaatcaaatc gaatatacgt ttattctcaa aaatataatt aataaatgga tctaattcat
     6901 tgaagttctc tctccaactt ttctctatat ctatattttc taagtttaag aggtatttgg
     6961 taaactcttc attatacata ttaataagcg cattaatatg cggctgaata atttcttcaa
     7021 ataacgcttc cttagaagta aaatgtttat aaaaagcacc cgtagttacg cctgcggtca
     7081 ggcacagttg ccttatctta gtttgctgaa atccttcttt ttcaaaatgg cttagtccac
     7141 tttctattaa cttttggtga gttataatat aatctctggt ttctatgggt acttcctcct
     7201 aataagatga attaacttta ttatcttaca caggacttga agtttcaatc gttaacgttt
     7261 attactgaac tcattcctta gagctaaact aatgagaaag agcagaataa ctgatacaaa
     7321 aagcaatcct acaagaatta atagactcat ctaaatcatt caccttttct aaaataatcc
     7381 gagaaagatt taaaaaaatt atatccagct attaggataa gtcctatcat aatcactcca
     7441 atagccattt tcttatcctc cctaaaaata aagtctttta cttaaaacct tttttgtaat
     7501 cgtcaataaa ttttttccct ttatcagtta attgaaatcc tatatctaaa tagtcctcat
     7561 atctaagttt agccagcatc tcacttacag atgctggact aacacttaga acttttgcta
     7621 aatcattatt ttttaatttc ttttttcttt ttgataattc ctgaattgct agtaaataaa
     7681 ttagtatagt agtcgtttta atttgtacca tacctaccct ccaaattatt tcgaaaggcc
     7741 ttcagcaatt ttatccaggt tccatttcat catcgcatag taactatcac catcttgtcc
     7801 tttttctgca attgaatcag taaaaatcgt tgaatagata ggaatattgg tatcttttga
     7861 tactgttttc attggtctat catccacact gctttctacg aataaggagg gaacttttgt
     7921 tgtgcgtaat ttttcaacta agtgttttat ttgatctggt gttccttctt cttcagtgtt
     7981 gatttcccaa atgtaagcag aaggcacatt atacgctttc gagaaatatt taaagcatcc
     8041 ttcacttgta acaatcattt ttttatcatt cggaattgaa gcaaatttag atttagcttc
     8101 tttgtctaga gaatccaatt tttcaatata cttatctaga ttttctttat agaatttttt
     8161 attatcagga tctttttccg ctaactgttt ttcaatattt ttagcgtaaa taataccgtt
     8221 ttctaaattt aaccaagcat gaggatcttc cttccctttc tcactctgac cctctaagta
     8281 aataacatct atgccatcac ttgctgcaaa atagtctttg ttttcctctt tgttcgcatt
     8341 tttaactaat tttgtaaacc aagcatttcc tccagtttcc aagttaacac cgttataaaa
     8401 aatcaaatct gcttttgaag ttttttgaac atcttcaggc aaaggttcat attcgtgggg
     8461 atctttccca ataggtacga tactgtgtaa atcaatttta tcttttgcta tattttcggt
     8521 aatatctgct aaaatcgaat tagtaactac tacttttaat ttttcgttcg aagccacttt
     8581 atctttgtct ttactattta ttgaacatgc cccgaatata aaaagagcaa tcaaaatccc
     8641 cagacttccc agtactgctt tccattttct catcataacg atctctcctt ctttcttaaa
     8701 aatttttgtt ttggtgagat tagaaaacta attacaaata agaatgccgc tgttaaaaca
     8761 atacttgatc ctgctgctat gttaaaacta taaccaataa acaatccaag caatgatgaa
     8821 cttgctccga ctgttgcaga aataaatatc atagttttca agctatttgc atataaatat
     8881 gctgtagctg ctggtgtaat taacattgca acaattaata tagttcccac actttgcata
     8941 gctgttactg ctactaaagt taatagaacc atcaatagat aatgataaaa actgacattc
     9001 atccctattg cttttgccat taatggatca aaagaagtga ttaataattc tttaaaaaat
     9061 agaactacta taaccaacac taaaaccgaa acgccaattg taatccactt atcaatatct
     9121 tgtacagcta aaatatttcc aaataaaata tgaaataaat ccgttgagct atttgcaact
     9181 cctataagga taactcctag tgctaaaaaa gaactgaaag taataccaat tgctgtatct
     9241 ccttttatta cactattgct ttttataaaa gtaataatga tagaagctaa tagaccaaat
     9301 acaatcgcac caataaaaaa attaatacct aaaatataag aaattgctac tcctggcaaa
     9361 acagcatgag aaatagcatc acccataaga gacattcctc ttaaaataat aaaacaacct
     9421 actgttccag aaacaatccc tataactaca gatgtaatta gagcattctg taaaaagtga
     9481 aagtcataca gaccattaat aaaattttga agcatattat tccccctctc caataaatat
     9541 agtatctcca taggcttttt ttaaattttc ttcattaaat acagattcta cacttccgtg
     9601 agcaattaga tttcgattca aaagaataat attatcaaaa tacttcttta ccttgcttaa
     9661 atcatgatgc acaattagca ctgtttttcc ctgatgtttc aattttttta acgtatccat
     9721 aattattcgt tcactaacta agtctatccc tacaaatggt tcatctaaaa atatgaaatc
     9781 cgcattttga gcaagacatc ttgctaaaag tacacgttga aattgtcctc cagataattc
     9841 tcctatttga tgattagaat attttcccat atcgaccttt tccaaagcct ttgaaactct
     9901 ttgccattct gcatttttaa ttctttgaaa aactttcatt ccggcatatg ttcccataga
     9961 tacacattct tttactttta ttggaaaagt aaaatcaata tcagcttttt gctctacata
    10021 tgcaatcttc accagttttt tattgatatc ttcattatta atggagacct ctccctgatg
    10081 agggataata tttaacattg ctttaagcaa tgtggatttt ccagcaccat ttggaccaat
    10141 aatacctgtt aaagcacctt cttgtatttt taaagaaatc tcgttcaatg ctaaaaaatc
    10201 gttataacaa acattaagct ttttcacttc taacataact ttctccttct aaatgataat
    10261 gattatcacg atcatatggt atacaaaaat tatattttag tcaaccctaa attttatagt
    10321 ttagattttt ttctgttgct gaagattaga atgacaaaac tatttgaaac tacgcgtata
    10381 gtaagtagct ttcaaaatat ataaaaaagg agcgttctca aatgagaaca tgctcctaaa
    10441 actactttat tggcgtatgg aaaaattata cttgaagtgg atagcgggtt gaataagttg
    10501 ataagcatca aaacatttat aaaaattatt caagactatg tctgttccat atacaaatat
    10561 gaaaatcagt aaactccatt taactttttt cgtagtgtgt ctttttatac atgtttatcg
    10621 ttattaatag tgaataggaa aaaaactatt tttgatcatt gacaattaaa tacaattttt
    10681 gaataacgat ccctgctatt cgagctagaa actcaagtac gccatgacct gattagcgac
    10741 aaatacttag agatttcttt ttgttctggg aaaacaaaat gccatgacaa tagcagcaaa
    10801 taatgatact tccgtaaggc tcgccgtaaa ctggggagag gtgaaattcc tatgtgctta
    10861 cccaaagcaa ttcaaaaatt tcataactac tattttagga gatgatttta tagaatccag
    10921 agtctacctg aataaaaaac aaacctgtgc atttctagga atcgataata atactctaga
    10981 taagtggatt cgtatatata atttcccagt aataagagtt ggtggtgtag ttcgctttga
    11041 cgtagaagat attaaagact ttatgaatac atttaaaata acttgaaatt ttcttctcgc
    11101 cgcatatact aaaattagtg aaatatactg ccttactttt tttaaggagt gaggattttg
    11161 cctacaaagc tttcaaatgg gaaatataaa actaatctga gatatccaaa aaggtttaaa
    11221 gaaataacag gaattgcatc agaaaagtat caaaaaacat ttccaaatag gcaattggcg
    11281 attaaagccg aaaatgatat gaagaagaaa atagaaaaag ttttgagaga ggagaatgca
    11341 aactcattag aattaaaagg aaaaataacg tttaaaaaat tttacgaatc taaatggctt
    11401 cctagatatg aattaggtca aacaattaga tcaaacaggc ctcctagtga tataaccatt
    11461 agtaatacaa aagatatatt tcggttgcat attttaccta tgtttggtga atacgcaatg
    11521 aattatctaa atatcaatac tgagataata tcagatgaat taaccaaaaa atcgaaagaa
    11581 tatgcaaata taaaaatcat aaaaggatat gttcgttcca tgttcgatat tgcagaaata
    11641 ctaaattata tcgaatttaa tcgaactact aaaattattc aaagtatcac tgcacctaaa
    11701 aaaaatgctt tagaagaaaa aagaatacaa gaaggtaagc aagcactatc atcaaaagaa
    11761 cttactaatt ggatagaagc agtaaatgat gattttaata atcatttact tacttttcat
    11821 gattacacac tttttatgat taccctgtat cttggtgata gaaaaagtga gacttacgct
    11881 ttgcaatgga aatatattga ttttgaaaaa caaacggtcc gtcttaaaca tactttagat
    11941 aaatatcaaa gaaaaaagtt tacaaaaggt cgaaaagata ccgtcattca agtaccagaa
    12001 gttgtaatga cattgctaag tgaatggaaa tcggtacaag ctgatcaatt gttgaaatta
    12061 aaaattaagc aaacgttaga tcagtatctc tttacttata ctaaaccatc tggagaagta
    12121 aattgtcctg ttcatgccga ttacctaaac tatcgaatca attcaattaa aagaaggcat
    12181 cctgacttag ttcatttatc tccacacaaa ctccgtcata cttatgcaac tatagctaga
    12241 caaggtggag cagatatgaa tcaaatttca aatgctttaa cacactcaga tatttctaca
    12301 acaaagatat atgtgaacac accagatata gttgataagg ctgtttttga agcttttcag
    12361 agaggattga ataagtgtga ttgatatgtg ttcttaaatg tgttcttaaa aacaaaagaa
    12421 gcactttcca aataattgaa aagtgccaga aacgttgata tataaaggat attaacgttt
    12481 tgagaattgt gaagctttac gtgctttctt aagacctggt tttttacgtt cgaccatacg
    12541 tgcgtcacga gtaagtaagc cagcacgttt aagagctaaa cggaagtcag gatcaacttc
    12601 tagcaatgca cgagcgatac catgacggat agctcctgat tgtccagcgt atcctccgcc
    12661 gtttacgtta acaaatacgt catatgcgcc ttttgtttca gtaacagcga aaggttgatt
    12721 gattacttca cgcaagtcag catgtggaat gtattcttca acatcttttt tatttacagt
    12781 aattttgcca gttcctggta ctagacgtac gcgagcaact gcgtttttac gacggcctgt
    12841 gccgatatat tgaacttgtg ccaatgaaat tccctcctat tagattaagt ttgtgatgtc
    12901 taaaacttct ggttgttgtg ctgcatgtgg atgttctgct ccaccgtata cgttcaattt
    12961 tgtaaattgt ttgcggccta aagtgttttt aggaagcata ccttttacag aagtttcgat
    13021 caaacgacga gaatttttag cacgcaattc gccagcagtg attgatttca atcctcctgg
    13081 gtagttgctg tgacggtaat aaattttgtc agtcgctttt ttacctgtta atttcacttt
    13141 gtctgcattg atgacgatga cgaaatcgcc agtatccaaa tgaggtgtga aagttggttt
    13201 atttttacca cgtagtacag atgcaacaac tgttgaaaga cgtcccaatg gaacatcagt
    13261 tgcgtctact acataccatt tacgttctac ttcgcctggt ttggccatat atgttgtacg
    13321 cacgttattt tcctccaatt taagttctga tgtttgacat acacttcgag tttccggggc
    13381 tctttgtggg gcaaacaata ccatttaaca tattacccgc taatctagtc aatgtcaacg
    13441 tttttctgct tgattgaaaa atttatatga aactttactt cttttctttc ttttatataa
    13501 aagggtcgtt tcctgtcaaa atgaaaaaca aaaagaggct gtgacaaaag tcttgtcaca
    13561 accccttatt ttgaataaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat
    13621 ccataaaaca taagaagctg ttttttaaaa ttgtttcttg tgactcggag ctgaacggca
    13681 ggcttacaac ctcttggtaa acggtgttca atcagctgac cctcggtatt agctcaaaaa
    13741 acggcaaaat atgaggaact attttgacgt ttgtttctct aatactcagg tcaaaacgct
    13801 gatttcacaa cctctttttc ggtcaagttc aacaacagtt tttacgcgtc gatctctggc
    13861 gctttgccca aatgagcttg gatcatccag atacgttttt ctgtggctgt tttgaattcg
    13921 ataaagatat cttgtgttgg atcgtcgcct tcttctccag aaacttcgat tcctttttgg
    13981 taaagatctg ctagataacg atagcctgca acaagtgttt ccaaacgttc tggaatagta
    14041 cgatcccagc ggccaatttc atcagggatt tttgtgttat ctgcaaattc acgcaatgta
    14101 gaataaggtg aaccgtctaa tgtgatcaag cgttcggata cttcatctaa ctgagcgttc
    14161 aaatcatcca tgaactcatc catcattggg tgcaatgtaa ggaaacctgg tcctctcatg
    14221 taccaatgtg tttggtgaac aacaacagac atttggctta gatctgcaac taattgattt
    14281 aatacttctt tggtttgttg gaatttcatc tttcttgcct cctaacattt tcttacactt
    14341 aaaataatag tagactttca atgaaaagta caacaaaatc atttacctga gaacgatttt
    14401 cagttcgctt ttttatccgt aaagccaatc atttttcaga atcagctaat tcttctcgca
    14461 gttgagcaaa ttgctgataa gtctgtgcta aatgcttttg tgtcgaaaca aataatggat
    14521 agattttctg atatttttta tgatcagttt cttgtactcc aacttgctct ttcaattcta
    14581 tcaattcctg tgcttcactg atactttgga tctttccgat gcttctccag cctaaaatca
    14641 cagctcctaa tgcggatgct tctgtgacag tagggaaaac aagcgggtaa cccagtacat
    14701 cagccatcat ctgacgaaaa atcggagatt gagcaaaccc gcctgtagca cggatctctg
    14761 tgagctgatt ttcctggcaa actcctttta aaatccgata aaggttgaga cagatccctt
    14821 ctagaatcgc cttcatcatt tctttttctg tatgttgccg ctgtaaaccg ataaaactgc
    14881 caaacgcatc tgcgtcccaa agcggtgctc gctcaccaag taaataggga tgaaaaatca
    14941 gaccattggc acccgctggc acaaaagcga tagccgaaag cagtgcatca tatggatttt
    15001 ctcctttttc tttagcagat tgaacgactt cctgtaaaaa tgtatgccct gcccaatcaa
    15061 agatgccagc accattgcta gtcgctcctc ccactaccca atgtttctga tccagaacat
    15121 aacaaaacgt ctccatttct ggatggaact gaggctgagt agtaataaaa cgaagtgctc
    15181 cgctcgttcc tacggtcaaa gcggcgatcg tatcaccaaa agcacctaac cctagattag
    15241 aaagcggccc gtctgcccct cccaaaacaa atgatatatc atttggcaaa cctagttctg
    15301 ctgctgtttt tgatggtaga cctgttactt gataggtgga aggaaagact tgcggcaatt
    15361 gctctattcg aatgttcaag tagtcaagca cttcttctgc ccattggaat tcatggatat
    15421 caaaatatcc agttcctgaa gctgttccgt agtcgctgat cagttcgcca aacaatctat
    15481 aaaagatata gtctttgata ccgacaaaat aagcagtttt tttgaataat tcaggttggc
    15541 tgttttgcag ccacttgatt ttgttcaatg gtgtcattgg atgaatcggc gtcccagtct
    15601 gccgataaaa ttctttgcca agcacggtat atttcagttt ttctgcttcg tccgctgctc
    15661 ggttatctgc ccaagtgatc attcgagata acggtgtttt ctcttcatcc aacaatatca
    15721 ggccatgcat cgcagaggag aaggagacaa attgaatgat tgtgtctgaa ggcaactgtt
    15781 taattacttc ttttataacg gtgcagacac cttgaaagat caattcctga tcttgttctg
    15841 ccatcccttg tgtttcttgg atcaaagggt agcgaaaata agcggatgct gcttcttggg
    15901 cttcatctgt gaaagcaact gctttggttt gtgtggttcc gatatctaat ccgattgaaa
    15961 cgttcatttt ttcacctcaa attttatgta tgtacaaaaa tttagtttaa gcttgcgtat
    16021 cttggttttg aaaaggggga gatggcgtgt tactttattt tatcattggt tgctgtatcg
    16081 gatcattttt atgtttaacc gcccagcgga ttccgttagg tcactctatt atttatccta
    16141 gatctcattg tgtcaaatgc tgtcgttcgc tttcttggta tgaattgatt ccgattctct
    16201 cgatcatcgt ccaacgattt cgctgccgct attgcaggtg tcggttgccc ttctactatt
    16261 tgttggcaga agccttatgt ggtggtcttt ttgcctggtt ctttactttt tcttccgcaa
    16321 gaaatttttc gacgttcata tggatgcttt cggctttgct attttcgttg atggatttat
    16381 tttacttcat ggtccattct cacacgctat tctgttcttg ggcaatttta tggatttttt
    16441 ggttgcaggc cggcgtcttc caatggcaaa gtgtgctgtt aacgtttatc gtaggcggtg
    16501 tgtgccttcg ctatggtcaa tcctatctcg gagctggcga tactctgttg atgctttctt
    16561 ggagtggtgg cttgtctcta gaagagttgc ttcagatctt gtttcttgct agtctgtttg
    16621 gaatatccgt tttttctgtt tactttatct gtcataaaaa aaagttggaa aagctgcctt
    16681 tcattccttt tttgagcagt gcgttgctga ttgtccttca tcttaggtat tgaaaaaagg
    16741 ttgtgacaaa cgacttgtca caacctctga tatatagtgt tctcttacca aatccttgtg
    16801 acttggagct aggcgacagg ttcacaacct ctccactaaa tggtgttcaa tcagctgacc
    16861 ctcagtatta gctcaaaatg ctggttttac aacctctgat atacggtgtt ccataagtag
    16921 ctttttcggc ttcttatttc ttgacgcccc aatgtcgacg aaggaaacct gcacgtccag
    16981 aaccttctcg ataaagattg taatggagct tgtttttctt gtaataatcc tgatggtaat
    17041 cttctgcagg ataaaattcg cttcttggtt cgatttttgt cacgattggc ttatcgaatc
    17101 gcccgctgtc ttgcaatgct tgttttgaac gctctgcaat atttcgttct tcttcatcat
    17161 tatagtagat cactggacga taagaatcac cgcgatcagc gaactgtcct aatgcgtcag
    17221 tcggatcggt ttgctgccaa tagatttcca ccaactgatc ataagaaata acttctggat
    17281 catattcgat ctctactgct tctgtatgcc ctgttgtgcc tgttgttact tgttcataag
    17341 ttggatttgg tacatgcccg ccagtataac cggatgtcac agaaatgatc cctggctgct
    17401 cttcaaatgg tttgaccata caccaaaagc atccgcctgc gaaaactgct ttttttgtca
    17461 tataaaatcc cccttttttc tcatcatacc ttgatttcca aaaggaggat aacgttttgc
    17521 ttgtgacaga aaaaacggaa actattatat acgtgatacg gcttctgata aatgccaaaa
    17581 cgaacgtact tgatcaaaaa cggttaaatc aggaatcaca tattcagcag aaggaagatt
    17641 tatgttttca cctattccca atacatgcat gttaccagcc aaagctgctt gacaaccagc
    17701 ttctgaatct tcaatgacca agcatgcatt tggcggtaca ttcaattgct gtgctccttt
    17761 gagaaaaact tctggatcag gttttgcttt tgatacttgc gtgccgtcaa taaggacatc
    17821 aaaataactt gtcaaattcg ttttttctaa aatgaggcgt gcatttttgc ttgctgatcc
    17881 caatccgatc ttgatgtttg tttttttcaa gtactcaaga acagctattg caccaggcaa
    17941 aacggatgtt tcatccattt tttgaatcgc ttgaagatat agattgtttt tttgctcagc
    18001 aaaggctgat ttctcttttt ctgagaattt ctgttccatt tttccaaatg acagcaatcg
    18061 ctctagtgac tcggttctgg atattccttt cagttgctcg ttttctttct cagtgaatgg
    18121 aatggacagc tggttagcta aaaccagcca cgcttcgtaa tgataacgag ctgtgtctac
    18181 aagaacgcca tctaaatcga atagtgctgc tttgattttc atcgattacc tccttgaaaa
    18241 tgaacgactt gaagtaaaga tgcgatgttt ttagctgtgc gatgaagatt atcagtggct
    18301 tgctgtaaag cttcttgtaa aggcacagct gcaggaagga tcggaaaaac ggccgaaatg
    18361 ccaacatctg ctgctttttc gcttccttta ccaacacttc cgcaaatagc tatcacagga
    18421 acgccctctg gtactcttct agcaatccca actggcgctt ttccttttag actttgtcca
    18481 tctagacgtc cttcgcctac gattacgagg tctgcttgct gtacccgttt atcgaactcc
    18541 agtaaatcca atacgtagtc gatgccagag actattttcc ctgaagcaaa tgctaacagt
    18601 cctgcaccca ttccaccacc agcactactg cccgatgcct cgattacttg aggggcaaat
    18661 ctcatataaa aatcatgcat ggcttggtca gccactgcta attcattttc tttcaagcct
    18721 ttttgtggtc cgaaaacaaa agaagcgcct tcttttccgc ataaaagatt agtaacatca
    18781 gtaatgacag tgattttggc tttaatcagc tcaataggca cgttcgtttt atcaactgta
    18841 gcaattttac ctagattttc tcccagtgct tctacttttt gattatcttg atcgtaaaaa
    18901 gcataaccta gaccttctgc catgccgatg ccgccatcgt tggtactact tccgccaacg
    18961 ccgatgatga tgtgtcgaac accttgttgc aacaattgtc gtatcagttc tcctactcct
    19021 cgcgtggata acctaagtgg ctgttgttgg tttagtggga catctttcaa tccgacgatt
    19081 tctgccattt caaaaatcgc agtttgaccg tctgaagcgt actggatttc tttgtttttg
    19141 ccaaatgcat tagtaacgac tgctgtttgc cgcttcaacc ttaaactgtc tgctaatgat
    19201 gaaaccgttc cttctcctcc atcgcctata ggtagcagat catattcagc ctgaacaaaa
    19261 acttcttgaa aacctgattg aatggcttgt gctgcctcta aggctgtcag actttctttg
    19321 aaagaatctg gcgcaatcag aatacgcatt attttcccct ccgttccaag atcagttctt
    19381 tttccagctg gtattggtct ccattgacaa aaataggtaa tgtttctttc gtctcttttt
    19441 ttattactat ttgttttttt gtcaaaacaa atagcaacct gtgatttttc caaataaaag
    19501 gaaaagaaat tttttcccat gctgaaggaa gtcttggaga aatagaaaga gttccattcg
    19561 ttactgtaat acctgaaaaa ccaaatataa ctgctagcca tagtgctcct aatgaagcag
    19621 catgaattcc gtcatctgat gaatgcggat ctggtcctaa atcaatcagg cacgcttctt
    19681 ggaataaatg atacgcccat tccatttctt ggatacggca agcctctatg gcgtgaatcg
    19741 ctttactcaa cgatgagtcg tggattgttt tgttttcata gtaatgcaaa ttcttagaaa
    19801 cgattggttg agaaaaatac tcaggtaata agtataacag catgacaaga tctgcttgct
    19861 tgctgacttg gagttcattt acttcgctac gggcataatc aagtaaaata gactggctgc
    19921 caggacgttc tttgtaagtg gtcaaatcaa taactggctt actgagatac gtgtcatctt
    19981 gcggaatcag ctgttctttg ttaggcactg gcaaatataa cttatctaaa aaattttgac
    20041 attctttcaa aaaaagcgga tcgccagctt gatatttaac agctaaggcc acattataat
    20101 gcgccagata gttcgtatat gcattattgt cgatatattc tgtgtattca tctggaccaa
    20161 tgactctgtg tatctctaaa tgatcatcga cttttacagc tctgctcagc caaaaagctg
    20221 ctgtttcatg aatgagcgtt tttccctttg tttctaaaaa cgattgatcc tctgttgctt
    20281 ggacatactc ttgaacggca taagcaaggt ctgcactgat atgatgttct gcggcagctg
    20341 aagcaacttc ttgtcttgtt ccggttttga tattcaatgc agcaaatttg ggtgtctctt
    20401 cctgaccagt gtgtgcgctt tcccatggga aaagtgcacc ttgatagtga taatctcttg
    20461 ctttttgccg tgcgccttct aatcgatcat aacggtacag cagcaaattc tttgctactt
    20521 caggaaactg atataaaaag aaaggcagga taaaaatttc cgtatcccag aaaacatgcc
    20581 ctttataacc ttcgccagtt agtcccttcg cccctacact aacacttgga tcattgcggg
    20641 gagtcataat ctgtaaatgg taagcagcga agtccatcgc taactgatcg aaagaagaat
    20701 cgccttggat cgttatccga tgctgatgcc aaaaatctgt ccaataagac atcgactgct
    20761 ctaaaatttc tgtataacta acgcttttct gggcttgggc aatacttttc ttaaacgggt
    20821 ctgtttgctg aagagaggta gagagcacga tgatcttctc cagcgtaaag gacgtatttt
    20881 ttctgatctt ttctgaaaag gaagcggtga tccggcgatt ttttgcttgg aatacacctg
    20941 ctttattgaa tttagctgca atcccaatat caattttact ttctgttgtc tggtaggatg
    21001 cactgatcaa atcgtcacca aatactcgca gttctttttc aaccaactgc tgtgtaccaa
    21061 aattggtttg ctgtccatcg atacccgttt tcacatctac ttggcagtct tcggtcaacg
    21121 gcttgatgac cactttcagg gctgcctgat ggagattatt ttgatcagca acacgacaag
    21181 taatgacttc cacttttttt ccttgagcag tttcaatgag cgcatagcgt ttaagctctc
    21241 ccatttctaa atctagtgcc cgctcgtatg caagaacttt catcgtctca agagaaaaat
    21301 gttcaccgtc gatttttatt tcccatctga tgacatctgg gagattcact aactcggctg
    21361 attctgctcc taaaggtcgg ttatatacac cagctgcaaa cattcccctt acttgattcc
    21421 gatagtcttc ctcctggctt cctctgatac caaaaaatcc atttccaatg gtcatctgac
    21481 tcgcatactt attgatatac tctaaatcaa actgtttttc ttctactttc ataaggtaat
    21541 aacttctcct gtttctgccg aacgatataa attttccacc agattctgga tgacatatcc
    21601 ttgccagcca tctgcaatca tctgagtctg gtcaccgagc gcccgattca caaaatgagc
    21661 catacttagt tcatgtgggt cattaactgg cgtggtcttt tgtaaggaaa ccagctttcc
    21721 atctacatct tcgaatagtt cgcctggata cagattagca cctgcctgat cgccacaaaa
    21781 agccacattc agtttgtctt ctctgatatt caaagcaaac gaagtattca gttggatgat
    21841 cttgccgttt tctaattcca gtgtgccaaa aattgcatct tctactgtga atttgcttgg
    21901 atcccatttc ccgaatttcc ctgaattttt tttcgtacca attttttgga agctgtacgc
    21961 ccgaacgctt tttactttag gaaatccagt aatatacatc gctgtatcaa gcatgtgaat
    22021 gccataatcg atcaaaggcc cgcctccttg aagttctttg tcgataaacg ttccccagcc
    22081 tggaacacca gaccgacgaa gcgcattcgc ttctacgtaa taaatatccc cgagaacatc
    22141 aagatgattc ttgatcattg ttgcttcgtc agaaaaacga tgctggaatc cataagccaa
    22201 aatctgcccg tttttatcgg ctgtctctgc catctttctt gcttgatcag ctgtcatagc
    22261 tggtggtttc tcgcacatca ctgaagcatt agcttcgagg gcagccaata catgttcgaa
    22321 gtgaaagcga ttgggtgtac agacactcac gatatcggga gaaatctcat tcagcatttg
    22381 tgtaactgat gtatacgcat gagaaaatcc tgtacgtcgg caaaagtctt ctgctcgctg
    22441 gctgactgga tcaaccacag cggccaattc taagacatct gatctttttt gataataagc
    22501 agcatgtact ttttctgcta cttgtccagc gcctatgatt gctactttct tcatttattt
    22561 ctccctcctt tgcttattta cttattgtta ttctttctct attctttctc taaatcattt
    22621 aaatgacgcg ctgtttccag acagtttttc aagtattgca atgattcctg ataaattttc
    22681 acttcatttt ctcctcgcaa acgacattca tataccatat aaccttcata ctcattttga
    22741 agtagcgttt gaaaatggcg agtaaaatca attgcgccag atcctggttg gtagcgatga
    22801 ttatctgcta aatggatatg acccacatac tctttttgcg tagtcaatgc ttttgaaata
    22861 tcatcttctt caatattcat atgataaaaa tcagcgatga tctgcacatg tttcaattga
    22921 ttagagtcaa tgtaatgacg cgcatccgct aacaaattga tcatatggtc ttggtatcgg
    22981 ttcaaaggct ccaaataaat aaccgtacct gtttcttcgg ctactttatc taaataacga
    23041 agtgattcgc tgactgcttt atagtctcct tctttgcttc ggggtgaaac catcggcggt
    23101 aatcgataag tgaacattcc ccaagctgct gggataacga ttcctttgcc tccaacctcg
    23161 cttagtgcac gcaaaatctc agcaatttga cgtacaccat taagacgccg ttcttcgata
    23221 aagtcaccga tccacccatc gtagccgtta caagctgtca ctactgggat ccctgtttct
    23281 ttgattgcct gtttgactgt gtcgatattt tctattaaca acttaccgtc gatttcgtat
    23341 tcttcaaatc cacagtcttt gataaatcta aatttttcta aaatgtctct tgggaaaaat
    23401 gcttgatctt gcgtaccgaa tttcattgtt ctcctcctcg gacttgaacg cccattttga
    23461 tactttcttc tgggtgctgg tctacataac gcatgtaagc ttctgcgctt tctgcaaatg
    23521 gcacaactgg atcaatgatt tcttcacaat ccaagtaacc gttcatcaat aattcccaac
    23581 atgtgtcctc gattcgttta cggttccatc ttgggtaatc tgggtttggt tcactagatg
    23641 ctcgagcaaa gacgattttt gcattattga aatgtgcttc gcgaccaaga ttaaacccag
    23701 ctggaaaagg tttagcaaag gcaacataag aaatgatccc gccatagccg atccctctta
    23761 aagaagcttg taatgcggca gcactgccac tcgtttcaat aactgaatct actccacgtt
    23821 tatctgtcaa ttcctttaga cgaaaaccga catctgcttt cataggatct agcgtgtaat
    23881 cggcaccgta tttttctgct aattctctac gctgcgctat gggatcgatc ccaacgatgg
    23941 tagtagcacc tgctttttta gctaattgaa tcgcaatttg cccgatagcg cctaaaccga
    24001 agattgctac agcatcacct gctcgaacat gtgcttcccg gataccacct aaagcgaatt
    24061 gagcaggatc gtaacatact gcatttttcc attgatccaa ccgatcaagt ttacgtagtt
    24121 tataattatc aacaccgttt gtaatcagcg tttcacgaat gggtccataa gtgcagacta
    24181 actctccttt ttgatactct gtcaccaaac tgcccgtttc ttcgatcgta ccgacaatca
    24241 tatttcctaa ttgaaattcg ccaaatacca caccgcgttg gcttccttct tctcttggca
    24301 agaataggcg ccattcttct gaaaattctt catcgataaa tggacttaat cctctaaagt
    24361 cgacaatttc tgttcctggt tttggagtag cgaaaattac tcggatacgt acttcgttct
    24421 tttcgatctc acggtcttcg taatctttga atccggctat tcttggttct aatgcaacta
    24481 attttttcat cttttctttc ctctttcttt tctttttatc cttttacgcc gccttcgact
    24541 aaatcacctt taataaatcg ttcagataac gcatacataa tgatgactgg taaagcggtc
    24601 accaatgaag cggccatcat tcgtccccaa atgtagtctg gtgtactgaa taatgtgttc
    24661 aaaccgattg gtaaagtaaa tttttcggag gtcgacagga aaatcgaagc aaataaataa
    24721 tcattccacg aaaccataaa gcaataaacg aagacagaaa tgattccaga aatagccaat
    24781 ggtataatga tccgaataat gatctggatc cggctaagac catcgatcat tgctgcttct
    24841 tcaatatcag tcggaatcgt gtcaaaataa ctttttagca tgaatatcgc agtaggaagt
    24901 gtttgaacga ccatcgtgat aatcagtgct gtattggtat catacaaacc tagaccggaa
    24961 atgattttaa ataatggaac aacgatcaaa ataccggaaa acatataaac ggtgtaaaag
    25021 ctagcattga ttttcgagcg agctttgaag cgtagttttg acaaagcgta tgccccgctg
    25081 atcccaatca ctactgcagt tacggaggct atcaaggaga tcactaaact atttttgaaa
    25141 tacatgatga atggaaaaat ctcagcattg aaaatatcct gataatgttc aaaagtccat
    25201 tctttcggaa aaatagtttg tgtcgttgca attgcttctt gtgaatcctt gaatgatgtc
    25261 atcagcatga tcaaaaatgg aaacaaattg acagctaata gcaacaaaat cgtcgcgtaa
    25321 aataagattt tcctgctctt tttatttttc atttcaagtc caccttcttt cgtgagagca
    25381 agacgaccgc tgcaataatg atgaacaata gaatcgatat agctgctgct tttccaagat
    25441 cattgaacgc aaaggctgtc tgatacaaat aaacgcctaa gatattgact ttattcgtca
    25501 gtagatagac atctgtaaac atatagaaca tccagataga tcgtaaaaca atcacagttg
    25561 ctagtactgg tttgattgcc ggcagtgtca cgatcttgaa cttctgccaa gcatttgcac
    25621 cgtccatttc agctgcttca taaagcgttt tgtcgactgt ttgcaaaata gccaaaaatg
    25681 aaataaacgc atacggatag tatctccaga tagcaaacaa aacgaccata aagaagctag
    25741 caactggctg gtcgaaccac agcggtgctt cgttgaaaag ctgcaaccta tctactaaca
    25801 gatcattgac gattccataa ctattattga acatatattt ccaagcaaaa atcaaagaaa
    25861 tagatggcgt aacataactt aataaaatca gtgaacgcac cgttttccta aatctgaact
    25921 tgcggttaag caaaatagct acggctaatc ctaaagctgt actgcctgct acaactaaga
    25981 atgtgtaagc aactgttaaa cctaaagaac gataaaattc actatcactt aaaatttcaa
    26041 gataattgct taatccgata aaaacggcgt ctgagttagg attcagcggc atatcgaaaa
    26101 aactgatctg aatattggaa agcattggaa tcagcactaa aacagctaat aaaagcatgc
    26161 ttggtgctaa aagtcccatg gctaatttta agtcagaatg tttccttgga cgattcattg
    26221 aaacctcccc ctgataatct gccaatagct atctactttt ttcgaacacc attggcagaa
    26281 cttcttgtta tttcgtattt ttttgtgcgt cagctaaagc ttgatcgaca tcttcgtttc
    26341 cgacagttac ttggttcact gcttttccga tcacacctga gcttgtgata tctcccattt
    26401 tcgtgaaatt cttttccccg actagtccaa agacttgtac ttcatcaaaa gcactagcaa
    26461 tctcagatgg cagctcgcca aaagacttga tgacttcatt ttctttgtaa ccatccagtt
    26521 cgactacttg tttattcact ggttgagcac caccaggaga catcaagacc cacttttcca
    26581 tgtttttcgg ttgagataag aaagctgtga atgcttcagc agcctctttt tgtttctcgt
    26641 ctagaccaga agaaatcgtc aatccagata ctgtgccata gacagcttga tctttttgtg
    26701 ttgggatagc aaaaccgata ttttctgagt tccccgcttc ataaacagaa ggcaaaatgt
    26761 aagttgaata gattgccatt ggtgctgttc cattcatgaa agcatctttg atttctgtca
    26821 catcattaga gcctggcatc gtgtattggg ataagttttg ataaaacgac aatgcttctc
    26881 tcatcttttc cgtatcgatc gttacctcgc cttcatcatc caaaacattt gctccatttg
    26941 acaaagcaaa ttgagagaac gcttgctcac tcattgttcc ttcagcagta ggcatagcaa
    27001 ttccatattg tttattctct ttatctgtga aatgttgagc aatttttaaa atatcatccc
    27061 aattttctgg ttctgaaaaa ccagcttctg ataatttttc tttgtcatac caaatacctt
    27121 gaacccagcc gctaattggt gcagcaatat agctttttcc atcttctgaa cgaacaaggt
    27181 ttttggctcc gtcataataa ttatcttctc cagcttcctc gataacgttt tggactgccg
    27241 tctgatcaat cagttcatct ttatccatca ctttagcaaa atcctgactg acttccatta
    27301 cttctggtaa tttaccggaa cgagctaacg taacgatttt tgtattgaac gctgattctt
    27361 ctaccggcac ttgcttgatt ttgatgtctg gattttcttt ttcaaaatca gcaaccagtt
    27421 cattgatcac acttaatcgt tcctgttcga cagaagagtg catgaattga atcgttgtct
    27481 gttgtgattc gttagagctt tgactttcac cacaagcagt aagaagaaat ccagtagcta
    27541 gcagacaggt agtaacaata atttttttca tttttttcca cctcttattt tattattttc
    27601 agccacatat attcatacgg ctggaatatc aactttcgtt cttgactgca atggataatt
    27661 tgtccagaaa acaggtctag gtaatctcct tttggtaaaa gataacctgc tgattgattg
    27721 gaaaaattat ggataactaa gactttctct tccttcgctt cgcgaataaa actgataaaa
    27781 gaatctgctg agtccagtaa gtaaatcaaa ctattcggat ggaaggcttt ttcttttttt
    27841 cgaatagata gcatctgggt caattcacca tatactgctg ttcttaatgg atctgtctgc
    27901 aactctttct caatatctgc taacaaaaat ttttgccgat tgatttttcg attctctcct
    27961 gttttttcta cgcctttgta atcatttcga cttcccagca cactttgaat ataaattgcc
    28021 ggcacgcctg gtaacgttac caaaaatgaa tgagcagcta aaaagcgttt gcttctccat
    28081 tccgtcgtat ctgttggaga acttaacgca tctagatacg tagcgtttat ctcatatgga
    28141 ctggtcgttc catctgggtt tttcttgtag tttactcggg taccctcttt cttcagctgt
    28201 tccactaatt caaggatcgt tttttccggc agaatacctc gtaccggatt caatccgata
    28261 ccgtcatggg aggctaaaaa attaaaaaaa gttgtttttt tcgatgcgaa atcgatggtt
    28321 tttaaccatt ctttcaactt acttgtatta cccgaacgaa tcgcttgaag ggtcaaaggt
    28381 ggtagcgaaa actgatagac catctgtgct tcattctcgc cattcccaaa ataactgata
    28441 ttatctttgt gtggcacatt tgtctcagtg atcaataccg ttcctggagc tgcttcatca
    28501 gcgattgcac ggaataattg gatgatttta tgcgtttctg gcaggtgtat acaagaagtt
    28561 cctagttctt tccataaaaa tcccacagcg tccaagcgaa taaatgaagc gcctttttct
    28621 aagtaaaaca aaagaatatc gatcattttc aataaaactt ctggattttt aaaattcaag
    28681 tctacttgat cttcgctaaa agtcgtccat acgtgttttt ctttcccgtt tttgtcaatg
    28741 aacgttgtaa gtaacgggct ggtccttggt ctagtcacgc tgcttgtatc tgtatccagt
    28801 ggcaaatcaa taaaaaaatc tttgtacgta tcattcaagt ttaaatattc ctgaaaccaa
    28861 gtactttcgg cagacatatg attgcagaca aagtcgaaca ttaaccgtgt ttcgcttctc
    28921 atttcttcta tatcttccca tgtgcccaaa cggtcgttga ttgcctgata atttacaacg
    28981 gagaaaccat catcagatgt atatgggaaa aatggcaaga aatgtacaac tctaaatgta
    29041 tctgccaaat acttttgata aaatttctta aatgtacgca aagttggttg ctgctcttca
    29101 aaaaattgat ccccataagt aatcaagaaa atatcttctt cgtcccaatg cagtttgcct
    29161 gcctggcctt tcttctgctg ataaacactg actttttgtt caatcataga ggcggttttc
    29221 tcagacaaat ctgagtcgcg ataaatcgct tgtaaaagtt ttgtatacat cggattcccc
    29281 tttctttttt gggagcgttc ccaacgattt gatcatatta tggaaaacgc ttactgtcaa
    29341 tagcattttt ttaaataatt ttgatataca caaaaataca aataagaaag cttatgtatc
    29401 aataattaat agcttcttaa aaaaatatgg tctctcatga tcatgcaagc tattcctaaa
    29461 ttttcaaaat catagacaaa aaagaatgtc tattgtacta tggaattatt ctaaaacgct
    29521 cccaaaaagg aggtgcatat aaaattgaaa cctactattt atgacattgc gaaaaaagct
    29581 aatgtctcaa aatcaactgt ctccagagta ttaaataacc agccaaatat ctctgataaa
    29641 gcaaaagcaa gtgtacttca agcaatcaaa gaattgaatt atgaaccgag caaaattgca
    29701 agaggactgt caggaaatgg ttttgacgcg attctagcat tatctcatcg tacttctcat
    29761 acgacctatg gcaatccttt tttttcagat gttattcaat ccatttctgc cgtttctgaa
    29821 caaaataact tcgacttgat tctacaaacg gcaaaaacac cagaagaaga gctagaaaaa
    29881 tgcgcgaaaa agatccaaga aaaaatgatc cgaggaatta ttattttaag ctctacgatg
    29941 gatgaaacac ttctttttga attggatcaa tacaatattc ctattgtggt aatcggaaaa
    30001 gtcggaaacg actacacgaa catctactca gtggacacgg ataatttcca ggatagttac
    30061 gatttggtgg aaacactgat caagaatgga caccgtcata tcggctgtct ccatgctccg
    30121 acagaagttt atgtatccaa tgatcgtctc aatggttacc gtcaatgctt atttgatcac
    30181 cacctagata tccgtaatga atggattata gacggcggct ttcgcttaga agatagcatg
    30241 gcagcaatga atgatttaat gaagctacga gaaagaccaa ctgcgatttt ttctaccgat
    30301 gcattaaaat cattgagtat ttacaaagtg gcagaacaat ctggaaagca gatacctgat
    30361 gatctttcga tcgcaggatt cagtgatccc ttattgtcct ccctattttc gccacagttg
    30421 actcggatag acattccgac aaatcagtta ggaatcaaag ccacagaact tttattccaa
    30481 ctaatccacc accaataccc tgaagaaaaa agaatcattc tttcaaccga gattactctc
    30541 acagattcga tccaacaaca agaaatgtag aacagaatta taaaagaaca gaaacactga
    30601 ggataagtca gttactctgt tcttttttca atcactcatc cctcgttacc ccctcccggt
    30661 attttagcga atttttctta ttttccatct gttttagata gctgtggata actataatgt
    30721 ttgtgttaaa ctattttttc cacagggaag aaaaaactat ttttaaccac gttgtgtata
    30781 agtttttata gttcgttttt ttaaacacat ttacccttat ttcaatccat gttatttggg
    30841 gatacttatc tctaaaacat aaatagaata acagacatgt ctactggttt tgttttattc
    30901 gaggaattat tcaataatag ctagcaaaat attaaaaata accgctctat cttcccatca
    30961 atttattttg cattcaaaaa atgatctatc tttacttttg ttgattgact tctcttggaa
    31021 gatgatcgtt ccgatacctt aattggatac gaaccgatgt tgttaagatc ctttcccact
    31081 tttcttc
//
LOCUS       DACDPS010000027        30329 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.027, whole genome shotgun sequence.
ACCESSION   DACDPS010000027 DACDPS010000000
VERSION     DACDPS010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30329
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.027"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            155..382
                     /locus_tag="IWJ07_001310"
     CDS             155..382
                     /locus_tag="IWJ07_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAP9332900.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            398..1564
                     /locus_tag="IWJ07_001311"
     CDS             398..1564
                     /locus_tag="IWJ07_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAP9332901.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            1799..2842
                     /gene="hrcA"
                     /locus_tag="IWJ07_001312"
     CDS             1799..2842
                     /gene="hrcA"
                     /locus_tag="IWJ07_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAP9332902.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            2936..3499
                     /gene="grpE"
                     /locus_tag="IWJ07_001313"
     CDS             2936..3499
                     /gene="grpE"
                     /locus_tag="IWJ07_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAP9332903.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            3550..5379
                     /gene="dnaK"
                     /locus_tag="IWJ07_001314"
     CDS             3550..5379
                     /gene="dnaK"
                     /locus_tag="IWJ07_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAP9332904.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            5530..6696
                     /gene="dnaJ"
                     /locus_tag="IWJ07_001315"
     CDS             5530..6696
                     /gene="dnaJ"
                     /locus_tag="IWJ07_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAP9332905.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            6858..7019
                     /locus_tag="IWJ07_001316"
     CDS             6858..7019
                     /locus_tag="IWJ07_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP9332906.1"
                     /translation="MIIIVSEGGIDYVLDIVNEPMVLSYAMEQVERLLVCAGEQAMRL
                     VQLSEKTRN"
     gene            complement(7064..7801)
                     /locus_tag="IWJ07_001317"
     CDS             complement(7064..7801)
                     /locus_tag="IWJ07_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9332907.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(7803..9305)
                     /locus_tag="IWJ07_001318"
     CDS             complement(7803..9305)
                     /locus_tag="IWJ07_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9332908.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            9564..11558
                     /gene="uvrB"
                     /locus_tag="IWJ07_001319"
     CDS             9564..11558
                     /gene="uvrB"
                     /locus_tag="IWJ07_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAP9332909.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            11568..14384
                     /gene="uvrA"
                     /locus_tag="IWJ07_001320"
     CDS             11568..14384
                     /gene="uvrA"
                     /locus_tag="IWJ07_001320"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAP9332910.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            14647..15912
                     /locus_tag="IWJ07_001321"
     CDS             14647..15912
                     /locus_tag="IWJ07_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAP9332911.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            15916..16647
                     /locus_tag="IWJ07_001322"
     CDS             15916..16647
                     /locus_tag="IWJ07_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAP9332912.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            16782..17666
                     /gene="rapZ"
                     /locus_tag="IWJ07_001323"
     CDS             16782..17666
                     /gene="rapZ"
                     /locus_tag="IWJ07_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAP9332913.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            17663..18661
                     /locus_tag="IWJ07_001324"
     CDS             17663..18661
                     /locus_tag="IWJ07_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAP9332914.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            18695..19630
                     /gene="whiA"
                     /locus_tag="IWJ07_001325"
     CDS             18695..19630
                     /gene="whiA"
                     /locus_tag="IWJ07_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAP9332915.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            20198..20854
                     /locus_tag="IWJ07_001326"
     CDS             20198..20854
                     /locus_tag="IWJ07_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332916.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            21033..21598
                     /locus_tag="IWJ07_001327"
                     /pseudo
     CDS             21033..21598
                     /locus_tag="IWJ07_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(22297..22818)
                     /locus_tag="IWJ07_001328"
     CDS             complement(22297..22818)
                     /locus_tag="IWJ07_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP9332917.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            23016..26696
                     /gene="nifJ"
                     /locus_tag="IWJ07_001329"
     CDS             23016..26696
                     /gene="nifJ"
                     /locus_tag="IWJ07_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAP9332918.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            26885..27772
                     /locus_tag="IWJ07_001330"
     CDS             26885..27772
                     /locus_tag="IWJ07_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAP9332919.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            27769..28938
                     /locus_tag="IWJ07_001331"
     CDS             27769..28938
                     /locus_tag="IWJ07_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332920.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            28962..30317
                     /locus_tag="IWJ07_001332"
     CDS             28962..30317
                     /locus_tag="IWJ07_001332"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAP9332921.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
BASE COUNT         9948 a         5245 c         6715 g         8421 t
ORIGIN      
        1 caaaattgtt taggaagaat catgagtgta aaaaaatcat ccaagaagtc gattcttgga
       61 ttttttagtg gatttgttcg ccaaatacgt ttagtatgcg tatatgagag gttgtaagca
      121 aagcgttgtg gtctgagcac ttaaggagga atctgtgaaa atagcttttc atatttttgc
      181 agattttgac ttaatgccga aggtcagctt tgcgattacc gtttatcgag aggctgtaaa
      241 agcaccgtgt agctccgatg acaataagca agcattttta gagctaggtt tccttgattc
      301 taaatatgcg gcttatttca atgagttggt gcttgaacgc gattttaaga aggtcgaaaa
      361 caggcacgac ttttcgacgt aaggagaaag aatttcaatg atagaaacaa taaatcccgc
      421 agaaaacaag gtttctgcgt acattcatat cccattttgc gagcatatct gctattactg
      481 tgactttaat aaagtttttt tagaagggca gccagtagat gagtatgtgg aaatgctgct
      541 gaatgaaatg agattaatgc tggaaagtca tcctacagcg aatttggaaa cactttatgt
      601 aggaggcgga acaccaactt ctttatctgc taaacaactt gatcgactat tatcaggtgc
      661 tagagagatc ctgccttttc aaaatggtaa tgagtttact gtagaagcga atcctggtga
      721 tttgacccgt gaaaaacttc aagtgatgaa aaactatggc gtcaatcgtt tatccatggg
      781 agtgcagaca tttgacaatc gcttattgaa aaaaattgga cgtaaacata ctgcagaaga
      841 tgtgtatcaa acaatgcaat ttttagaagc agaaaatttt accaatgtaa gcatcgattt
      901 gatttatgca ttaccgggac aaacactaga aggatatcgg gatactttgg aaaaagcttt
      961 ggctttggat ctgcctcact attctttgta ctcattgatc ctcgaaaaca aaacaatgtt
     1021 catgaactgg gtacgacaag gaagactcga actgcctgat caggaaacgg aaaccagaat
     1081 gtttgaagag acgattgaag caatggaaaa gcatggacgt caccaatatg aaataagtaa
     1141 ctttgggctc tccggacatg aatctcaaca taacctcatg tactggaaca atgaccacta
     1201 ttttgggttt ggagctggtg ctagcggtta tcttggagat attcgttacc gcaacaaagg
     1261 accaatccaa cactatttac gcccattacg agcaaatgaa cgcccggtat tggaagaaga
     1321 aaaattgaca cgaaaaaatc aaatagaaga agaaatgttc ttaggactaa gaaaaaaaac
     1381 agggattact aaaaaacatt tctatgatcg ctatcaaaaa acaattgaat cggtgtacgg
     1441 tgatgtgcta agggaattag agcaagaagg gttacttgtt aatgaagaag accgtgttta
     1501 tttgacacca agagggactt ttttggggaa caatgtgttt gagcgctttt tatttgaaaa
     1561 atagtttgaa gaatatttgt tttttatcga aacttgaaaa gaaaaagttt agaaagtttg
     1621 acaatgaggt attgaagtat tgatataact ggatttacat caattattta gaattagcac
     1681 tcttgtgttt agagtgctaa aaaatggagt ctttctcttg acaggagaag ggcttcttgc
     1741 tatatttata agtgaattag cacttagaat atatgagtgc taatgagagg tgaggaagat
     1801 gttaacaaag cgacaaaacg acattttacg tctgatcatc caacactata cgagtagtgg
     1861 cgtgcccgtt ggttccaaaa cattaatgtc tgagggagtc aaggccagct cagcaacgat
     1921 tcgcaatgat atgaaagcat tagaagatga aggattgctg ttgaagactc actcttcttc
     1981 tgggcggatt ccatctgctt taggctatcg ttattacgtg gatcatttgt taaaacctgc
     2041 tcgcgtagca gaagatgatt tgctgctgat tcgtcagtct ctaggcagag aatttcatga
     2101 aatcaatgag attattaagc aatctgctga gatattatct gagctgacaa gctatacgac
     2161 tttttcattg ggacctgaaa taaaagaccg ccgactgact ggctttcgta tcgtaccctt
     2221 gaataatcga cagattattg ctatcgtcgt aacagatatg gggaatgtag aaagccaagt
     2281 gttcactctt cccgaatata tgggaagtca ggaactagaa aaaatggtac agattgtaaa
     2341 cgaccgtcta gtcggagatc ctctgatgac ggtgtaccat aaacttcgta cggagattcc
     2401 gatgattctt cataaatatt tccagacgac agaagggatg tctcagttat ttgacaccgt
     2461 tcttggtcat gtttttgaag aaaaggttta tgtcagtgga caaatgaatc tcttggatta
     2521 tgagccccgt caagatcttg atcagttcaa atcgatgttt tcttttatga aagactcaga
     2581 cggacttaca caaatgatca ctccgatgga tagcaatatt catatcaaga ttggtgctga
     2641 attaggcaat gatttattgc agaatatgag tatgattcaa gcaagttacg agatagatgg
     2701 tcatggaaga ggtacaatag ccttgttagg gcctactagt atgccttatt ctcggatgtt
     2761 cggtctagtc gatgtttatc gaagagaact agcagctaaa cttgctgatt actaccgctc
     2821 gcttgacttg tcaaactcgt aatgtgaata acgaagctat ttgcagctga tcgttctctc
     2881 tttcttagta agagcaatca aacagctgac tgaataatga aaggaagact gtatcgtgaa
     2941 agaaaatcaa gaagaactag ataaagaaat gacagatgct cagccagaac cggaaatcga
     3001 tgtcgaagct gcagaagatt cgggtatttc agaagctgaa gcggaagaat tcgaaacagc
     3061 gaaattaaaa gctgagttag aagaaatgga agataaatac ttgcgggctc gtgctgaaat
     3121 cgcaaatatg gcaaatcgag gaaaaaatga gcgtgagcaa ttacaaaaat accgttcaca
     3181 agatttagcc aaaaaattac ttccatcaat tgacaacctg gaacgtgcat tagccacaga
     3241 agtgtcagat gatcaagggg caggtttgaa aaaaggcgtg gaaatggtct tggaaagttt
     3301 gcgtaacgca ctggaagaag aaggaattga aaaaatccct gctaaaggag aagcctttga
     3361 tcctaatctt catcaagcag tacaaactgt tccagcgact gaagatacac cagccgatac
     3421 gattgtagaa gtactacaag aaggctacaa attacatgat cgcgtgttaa gaccaactat
     3481 ggtcattgtt gcacaataat tgtgcggata taaaacttac actttcaatt aaagaaggag
     3541 acgttaatta tgagtaaaat tatcggtatt gacttaggta cgactaactc tgcagtagca
     3601 gtattggaag gaaacgaagc aaaaatcatc gctaacccag aaggaaaccg tacaacacct
     3661 tctgttgtat cattcaaaaa tggagaaatc caagtagggg aagttgcaaa acgtcaagct
     3721 gtgacaaacc caaatacgat ttcatcaatc aaacgccata tcggtgaagc aggttacaaa
     3781 gtagatgtag aaggtaaatc atatacacca caagaaatct cagcaatgat ccttcaatat
     3841 atcaaaggat tcgcagaaga ctatttaggt gaaaaagtcg aaaaagcagt tatcactgtg
     3901 ccagcttact ttaatgatgc acaacgtcaa gcaacaaaag atgctggtaa gatcgctggt
     3961 ctagaagtag aacgtatcgt caatgaacca actgcagctg ctcttgccta tggtttagat
     4021 aaaacagatc gcgacgaaaa aatcttagta ttcgaccttg gtggcggtac ttttgacgta
     4081 tctatccttg aactaggaga cggtgtcttt gatgtattgt caacagctgg cgacaaccat
     4141 ctaggtgggg acgactttga taacaaaatc atcgactaca tggtagcaga attcaaaaaa
     4201 gaaaacagca ttgacttgtc tcaagacaaa atggctttac aacgcttgaa agacgctgct
     4261 gaaaaagcga aaaaagattt atctggcgta tcaagcacac aaatcagctt gccatttatc
     4321 acagcaggag aatctggacc attgcatttg gaaatgacat tgacacgtgc aaaatttgat
     4381 gaattaacag ctgacttagt agaacgtaca aaagtaccag tacgtcaagc gttaaaagat
     4441 gcaggtctgt cacaatcaga aatcgacgaa gttattttag tcggtggatc tactcgtatc
     4501 cctgcagttg tagaagcagt acgtaaagaa acaggaaaag aaccaaacaa atcagttaac
     4561 cctgatgaag tagttgctat gggtgctgct atccaaggtg gcgtgatcac tggtgatgtg
     4621 aaagatgttg ttttacttga cgttacacca ttgtcattag gtatcgaaac aatgggtgga
     4681 gtgttcacta aattgattga ccgcaataca acgattccaa caagtaaatc acaagtattc
     4741 tcaactgcgg cagataatca accagcagta gacatccatg tattacaagg tgaacgccca
     4801 atggctgctg acaacaaaac tctaggaaga ttccaattga cagatattcc agcagctcct
     4861 cgtggtattc cacaaatcga agtaacattc gatattgata aaaacgggat tgtaaatgta
     4921 tctgcgaaag atctaggtac acaaaaagaa caaaaaatca cgatcaaatc ttcttcaggt
     4981 ctaacagacg aagaaatcga acgtatggtg aaagatgcag aagcaaacgc tgaagcagat
     5041 aaagctcgta aagaggaagt tgacctacgt aatgatgtag acgcattgct attctctgtg
     5101 gacaaaacat tgaaagaatt agaaggcaaa gtggacgaag aagaagtgaa aaaagcagaa
     5161 acagctcgtg acgaattaaa agccgctgtt gaagcaaaca acatcgagga aatgaaaact
     5221 aaacgcgatg cattgaacga aatcgttcaa aacttgactg ttaaactata cgaacaagct
     5281 gcacaacaac aagctcaaga aaatccagaa gcagctcaag gcggagcaga tgatgtcgtt
     5341 gatgctgatt ttgaagaagt cgatggcgac gataaataat cagttcactg aatttcgaga
     5401 gaaggccaaa gttgctactt tggctttttc ttttgaaaaa tagtttgaag tatcggtttt
     5461 tgtttcaaac aaaatatgat atgataacaa gcgacgtgaa aatgatgaga accaatagga
     5521 ggaaagccta tggcaacaaa acgtgattat tatgaagtct tagggctctc aaaaggagca
     5581 tcagaagatg aaataaagaa agcttatcgt aagctttcga aaaaatacca tcctgacatc
     5641 aataaagaac ctgatgcgga agaaaaattc aaagaagtat cagaagcata tgagatttta
     5701 agtgacccgc aaaaacgcgc tgcatacgac caatacgggc atgcaggcac agatccaaat
     5761 tacggtgctg gtggagctgg tggcggattt ggtggattcg gcggcttctc tggtggcggc
     5821 ttcggtggtt ttgaagatat cttcgactct ttctttggag gaggcggtcg gtcagtcgat
     5881 ccaaatgcgc ctcgtcaagg tgctgatttg caatatacgg tcgacttgtc ttttgaagaa
     5941 gcgatttttg gtgtagaaaa agaaatcaag tataatcggg aagaaatctg tcatacttgt
     6001 ggtggaaacg gtgcaaaacc aggcacacaa ccaactactt gtcataaatg tcatggtgca
     6061 ggtacgatca atgtagaacg ccaaacgcca cttggacgag tgatgagccg tcaaacttgt
     6121 gatgtttgtc atggcacagg gaaagaaatc aaagaacctt gtccaacctg tcatggctca
     6181 ggacatgaga agaaagcaca ttcagttaaa gtgaatgtac cagctggtgt agaagaagga
     6241 caacaaatgc gtttagctgg tcaaggagaa gctggagaaa acggcggacc atttggtgat
     6301 ttgtatgtgg tttttcgtgt agaagaaagt gatatctttg atcgtgaagg ttctgaaatt
     6361 tactatgaat tgcctttaag ttttgtacaa gcagctttgg gggatgaagt ccaagtacca
     6421 actgtccatg gagatgtgaa attgaagatc cctgctggaa cacagacagg tacgaacttc
     6481 cgtttacgtg gaaaaggcgc tccgaagtta cgtggtggtg caacaggcga ccaacatgtc
     6541 aaagtaaaac tgatcacacc gaaaaatcta aatgaacaac aaagagaagc attacgtgca
     6601 ttcgctgaag ctggcggcat caaagttgaa gaacagcaag aagacggttt cttcgataag
     6661 tttaaagatg cttttggcgg gaaaaagcgg aagtagtttt tctagtaaaa atataaagta
     6721 gacttgccca aattttctat ttctagtaaa aacgctaagt cattaataat taaaaaccag
     6781 acgaacaatc atacgttcgc ctggtttttt tgtagttgtc atgtttgtac caacaaaaaa
     6841 gccagcttta gaataagata atcatcatag tttctgaagg tggtatcgac tatgtacttg
     6901 atatcgtcaa tgaaccgatg gtactttctt atgcgatgga acaggtagag cgattgcttg
     6961 tttgtgcagg agaacaagcg atgcggctgg tacagttaag tgaaaaaaca agaaactaaa
     7021 aatgcccggt cgtcatgaag cgaccgggca tttttggttt ttattaaata tttaatactt
     7081 tattcaagaa atcttttgtc cgttcatttt gcggattttc aaagatttgt tgaggcgttc
     7141 cgtcttctaa aaagtttccg tcgtcaatga acatgacacg attggccact tctttagcga
     7201 agcccatttc gtgcgtaacg ataaccatcg tcataccttg ttctgctaac tttttcatga
     7261 cgttcaagac atcaccgacc atttcggggt cgagtgcaga tgtaggctca tcaaaaagca
     7321 tgatgtcagg gttcatcgct agagcacgag caatagccac acgctgcttt tgtccaccgg
     7381 aaagcatttc aggatacata tcttttttat cttctagacc aactgtctgc agcaatttga
     7441 ttgctttttt ctctgcttct tctctagatt ctttcttcag gtccatcggt gctaaaatga
     7501 tgttttccaa aatagaaaga tgagggaata ggttaaagtg ctggaatacc attccgatat
     7561 gttgtctcac caagttgata tctgtgcttt tatccatcaa gtgggcgccg tcaataataa
     7621 tttcaccgtc agaaggttct tctaaacgat tcaagcaacg caaaaatgta ctttttcctg
     7681 aacctgaagg gccaatgata cagacgacat ctccttcgtt gatggacaca ttgatgtcgt
     7741 ttaatactgt gttatcgccg tattttttga ctaaatgctc aactaagatt ttttctgcca
     7801 tcttacttca ccttcttttc taaaactttt gctaattttg tcaatgctgt gatcaaaatc
     7861 aagtacatga tcgcaatgat caagtaaaca tacgtacttt gtgttgtacg tgcaacaatg
     7921 atcttaccag tttgcagcaa ttcgactaca ccgattgcgg aaatgatcgt tgtgtctttt
     7981 aatgagataa cgaactgatt gacgaatgat gggatcatga tgcgtatcgc ttgcggcaaa
     8041 atgatttttt gcattgtccg attataagaa agacctaaac ttctggaagc ttccatttgt
     8101 ccgacaggaa cggcgttgat cccgcctcga acaatctctg cgatataagc acttgcgtta
     8161 agagtcaagg tgataacacc agcaaggaag tcaggaatcg taaatccaac gataccaggc
     8221 aacccaaaga aaataaagaa cgcaagaacc atcattggaa ttcctcggat gatatcgaca
     8281 taaatactag caattgtgcg aagagcgcgt attggtgcta cactgaataa tccaaaaatc
     8341 acaccgatta ctatagccaa agcaaaggag atcaaagtta ggagaattgt tctccaaagt
     8401 cctttcaaca atgcttgata gttgttttgg ataagtccaa gaaatgttga ttcatcagca
     8461 gtggaagact cactggaagt ttctgaacca tccgctacat attctttaat aatcttatcg
     8521 tattcgcctg ttctttttaa ttctttcaat ccttcattga acatattaag aagctcagga
     8581 ttttgtcctt ttttaacggc aaatgcatat tttccgccag attctttttt gatcggtgtt
     8641 tccaattctt gtccttgttt gatcgcataa ccgattaccg gataatcatc catcattgca
     8701 tcgattgcgc caacttttaa cccgtcgtac aattgatcag cagcatcaaa atatttgata
     8761 gaatagccat attcagattc atggtcgctt aaaaattcag cactttcggt accaactttt
     8821 gctccgactg tttttccttt caaatcttca taagacttga tcgaagtatc tccttttttg
     8881 acagctaatt ggatcccgct atcaaagtaa gaatcagaaa aatcatagct tttctttcgt
     8941 tcatctgtta cagtcattcc ggcaatcatt ccgtctgctt gtcctgattc caaagcttgc
     9001 aaagcagcag agaaaccacg gtagtccatt cggatgttaa atccttgcag tttggcgatg
     9061 ttttccatca attttacatc gatgccttcg tactcgccct ttgcgttttg aaattcaaat
     9121 ggagcaaaag cagagtcact ggcaattacg tatacgtctt ttttgggctc tattttttgc
     9181 attgtctcgc ttgtgcttga ctctgccgtt gtagtgttgt cagcttgtgc aacatgtcca
     9241 aacgcaagtg tcacaagtcc tgtcattaag aaaacaaatg agtgaaataa atgctttctt
     9301 ttcataatca acctcctaaa tttccaatat tttattatta taggcgaaaa gtgctgaatc
     9361 agacaagact gtaagctcga aaaaatgaaa aaaatgcgag tttctaacat tttatgttat
     9421 aaaacgtaac atgcataata agataaaaaa gtggcgaaag tatgttcggt gtggtataat
     9481 tttttagtaa ggaatgcctt tgctttcctt ttctttttat tggtcgtaaa atgaaaagga
     9541 atgaatgaaa aggaggacga agaatgattg agagagatac ctcccgacac tttgagcttg
     9601 tctcgaaata ccaaccggct ggtgatcagc ctgaagcgat taaccaatta gtggatggcg
     9661 tagtcggcgg gaaaaaagca caaatcttat taggtgcgac tggaacaggg aaaacatata
     9721 cgatttccaa tcttattgag aaagtgaata aaccaacact aattattgcc cataacaaaa
     9781 cattggctgg tcagctgtac ggggaattca aggaattttt ccctaacaat gctgttgaat
     9841 attttgtttc ttattatgat tattatcagc ctgaagctta tgtgccttca agcgatacgt
     9901 atatcgaaaa agattcaagt gtaaatgatg agatcgacaa attacgacat tcggctacta
     9961 gttcattatt agaaagaaat gatgtgatcg tcattgcatc cgtttcatgt atttttggat
    10021 taggttcgcc ttttgagtac caaaaacaag tagtttcgat tcgacaggga gcagaactag
    10081 atcgcaatca gttgattcgt gacttagtca gtatccaatt cgaaagaaat gatatagatt
    10141 ttcagcgcgg tcgttttcga gtgcgcggtg atgtcgtgga gatttttccg gcttcaagag
    10201 atgaacgtgc cttacgtgtg gagttttttg gagatgaggt cgaacgtatc cgagaagtca
    10261 atgcactgac aggtgaagtt ttaggagaaa cggaacacgt ggcaatcttc ccggcgactc
    10321 actttgtgac gaatgatgaa catatggagc atgccgtagc gaatatcaaa gcggaattag
    10381 aacagcgttt aactgtcttg cgaaatgaaa acaaacttct ggaagctcaa cgtttagaac
    10441 agcgaaccaa ttacgatatt gaaatgatgt tagaaatggg ctacacgtcg ggaattgaaa
    10501 attattctcg gcatatggac ggacgtaaag aaggtgaacc gccttatacg ctattggatt
    10561 tctttcctga agattttttg atcgtggcgg acgaatcgca cgtaaccatg cctcaagtca
    10621 gaggaatgta taatggtgac cgagcgagaa aacagatgct agtagattac ggtttccgtt
    10681 tgccatctgc tttagacaat cggccacttc ggttagaaga gtttgaaaaa catgtcaatc
    10741 agatcattta tgtctctgcg acgccaggac catatgaaca tgaacaaaca gataccgtta
    10801 tccaacaaat catccgtccg acagggctgc ttgacccagt gatcgaagtt cgtccaatca
    10861 tgggacaaat cgatgatcta gtaggcgaaa taaatgaacg agtcgaaaaa gaccagcggg
    10921 tgtttgtaac gacattgacg aaaaaaatgg cagaagacct cacagattat ttcaaagaat
    10981 tagggatcaa agtcaaatat ttgcatagtg atatcaaaac gctggaacga acagaaatca
    11041 ttcgagatct tcgattaggt gaatttgatg tgttagttgg aatcaatttg cttcgtgaag
    11101 gattagacgt acctgaagtt tcccttgttg ctatcttgga tgcggataaa gaaggctttt
    11161 tacgtagtga acgttcctta gtccaaacga ttggacgagc tgctcggaat gaagaaggta
    11221 aagtaatcat gtacgcagac aaagtaacag attctatgcg tctagcgatg gatgaaacat
    11281 caagacgccg tacgattcag cagaagtaca atgaagaaca tgggatcgtt ccaaaaacga
    11341 tcattaaaga aatccgagat ttgatttcta ttacgaaaga atcggaagat gatacaaaag
    11401 aagcggtaca agtctcgtat gaagaaatga cgaaagaaga aaaagatacg ttattgatga
    11461 aattagaaaa agaaatgaaa aatgcggcga aagcattgga ttttgaaaca gccgctaatg
    11521 tgcgagatat gattttagaa ttaaaagctt caaagtagga ggacaacatg gctaacgata
    11581 aaattacgat tcacggtgca cgtgcccata acttgaaaaa tatagatgta acgattccac
    11641 gagataaatt cgtggtcgtt acaggtttgt ccggttctgg aaaaagttcc ctagcatttg
    11701 atacgctgta cgctgaaggg cagcggagat acgtagagag cctttctgct tatgctcgtc
    11761 agtttttagg acaaatggat aagccggacg tagatagcat cgacggattg agtccggcga
    11821 tttctattga tcagaaaaca acaagtaaaa atcctcggtc gacagtaggt actgtgacgg
    11881 aaatcaatga ctatttacgc ttgttgtttg cacgtgtagg acatccgatt tgtcccaacg
    11941 atcatatcga gattaccagt caatcagtag atcaaatggc agatcaagta atggctttgc
    12001 ctgagcgcac aaagatccaa gtgttggcac cgatcgtggt aaagaaaaaa ggacaacata
    12061 aaaaaatatt cgaacgaatc caaaaagagg gatacgtccg cgttcgagtc gatggtgaga
    12121 tctatgattt aagtgaagtg cctgagctgg agaaaaacaa aaaacatgat atcgcgatcg
    12181 tcatcgaccg gatcattgtg aaagaaggga ttcgttctcg tctatttgat tctttagagg
    12241 cggctctgcg attggcagac ggctatgctc tggttgacgt gatcggtgaa gaagaaatgt
    12301 tgtttagcga acactatgcc tgtccttatt gcggatttac tgttggcgaa ctagaaccaa
    12361 gactgttctc attcaatgcg ccttttggag cttgtccaga ctgtgatggt ttaggtgtca
    12421 aattagaagt cgatcaagat ttagtgattc cagatagaac gaagacactt agtcaaggtg
    12481 ctatcgttcc atggaatcca atcagctctc agtattatcc tcaaatgtta gcgcaggctt
    12541 gtgaaagttt cggtatcgat ttagatacac cattcgaaga attaccggaa gagcatcagg
    12601 atatcatctt gaatgggaca gatgatcttt tccatttcca ttatgaaaat gattttggtg
    12661 gcgttcgcga tgtagaaacg actttcgaag gggtattgaa gaatatcgag cgtcgttatc
    12721 atgatacgaa cagtgatttc acgcgtgagc aaatgcgtct ttatatgaca gaattaactt
    12781 gtcagacatg taaaggttat cgattgaatc cccaagctct atctgttcaa atcaatggaa
    12841 cgaatatcgg gcaaacaaat gagttgtcta ttcaaaaagc agtagatttc ttctcaaact
    12901 taacgttatc tgaacaagag aaagtaattg ccaaaccgat tttaaaagaa gtaaatgaca
    12961 gactttcatt cttggaaaat gtgggactag actatctaac tttgagtcgc gcttccggaa
    13021 cattatctgg gggagaagcg cagcgaatca gattggctac acaaattgga tcgaatcttt
    13081 ctggagtttt gtatatttta gatgaaccat cgatcggatt gcatcaacgc gacaacgatc
    13141 gactgattgg ctcattaaag aaaatgcgtg atcttggcaa caccctcgtt gtagtcgaac
    13201 atgatgaaga tacgatgtgg gcagcagatt atctgattga cgtcggacct ggggccggag
    13261 aacaaggcgg acaaatcgtg gctgcaggca ctcctgaaga agtagctaat gatgaaaatt
    13321 ctttgacagg gaaatatctt tcagggaaaa aatcaatccc tgtaccaaaa gaacgccgta
    13381 aaggtaatgg gaaagcaatc aagatcactg gtgcttcaga aaacaatctg aaaaatatta
    13441 gtgttgaatt tccattagga gaatttgtag cagtcacagg tgtgtctggt tctggaaaga
    13501 gtacattggt caatagtatc ttaaagaaat cgttagcgca aaaattaaat aagaattctg
    13561 ctaagccagg taaattcaag acaatttccg gctacgaaag tatcgaaaag atcatcgata
    13621 tcgatcaaag cccaatcggc cggacgccga gaagtaatcc agcgacttat acaagtgtat
    13681 ttgatgatat ccgtgggtta tttgctcaaa cgaacgaggc aaaaatgcgg ggttataaga
    13741 aagggcgttt tagtttcaac gtaaaaggcg gtcgttgtga agcttgtcgc ggggatggaa
    13801 ttattaagat cgaaatgcac tttttgcctg atgtctatgt tccttgtgaa gtatgtcatg
    13861 gcaaacgata taactctgaa acattagaag tgcattacaa aggaaaaagc attgctgata
    13921 ttttggaaat gacagtagaa gatgctgtag aattcttcaa gcacattcca aagattcatc
    13981 gcaaactgca aacgattgtt gatgttggct taggttatgt gacgatgggg caaccagcaa
    14041 cgacattgtc cggtggtgag gcacaacgga tgaaacttgc cagtgaattg cacaaaatct
    14101 ctaatggaaa gaatttctat atactagatg aaccaacgac aggacttcat agcgatgaca
    14161 tcgcccgctt gttgcatgta ttacaaagat tagtagatgc tggtaacaca gttttagtga
    14221 ttgaacacaa tctagatgta atcaaaacag cagattatat cattgattta ggaccagaag
    14281 gtggagaagg tggaggaacg atccttgcga ctggaacacc agaagaaatc attaacgtaa
    14341 aagaaagtta tacaggtcac tatttgaaaa aaataatggt ataaagagga tagatagctg
    14401 agatagttaa aaaaacataa aaaaatgaat ttttgcttgt tttattaata tcagtgttct
    14461 agaatcatta catggtagtt ctttctacac tgattggtat agacaaaaac ttcctataag
    14521 ttcaatccgt tcaaaaaaga attgagcaat cggaggagtt tcctttgatt gcttttttct
    14581 ttatggtatg atattccttg tgtagttgga aaagaaaaac aagagattat gagtaagtga
    14641 gagataatga tgaacagtat tgaaaatgaa gaatttatcc ctatattatt aggaagcgac
    14701 atgaacgttt atggtatggc acgttctttt aatgaagcat acggcaaaat ctgccaagca
    14761 tatgcttcgg atcagttagc ccctacccgc tacagtaaaa ttgtcaatgt agaagttatt
    14821 ccaggatttg ataaagaccc tgtgtttatc gagacgatgc ttcgtttggc aaaagaaaga
    14881 tattctgata agagtaaaaa atatcttttg attgcttgtg gtgatggata tgcagaattg
    14941 atctcccaac ataaacaaga gctgtcagaa tattttatct gtccatatat tgattattct
    15001 ttatttgaaa gactgatcaa taaagtaagc ttttatgaag tttgtgaaga atatgatctt
    15061 ccgtatccta aaacattgat cgtcagagaa gaaatgttag taaatggaca tttggaacag
    15121 gaattgcctt ttgaattccc agttgctttg aaacctgcaa acagtgtgga gtatctttct
    15181 gtccaatttg aaggaagaaa aaaagctttt attttagaga ctagagaaga gttcgattta
    15241 attttaggcc ggatatacga agccggttat aaaagtgaaa tgattgtaca agattttatc
    15301 ccgggtgatg atagtaatat gcgtgtgcta aatgcgtatg tagatgaaga tcatcaagtt
    15361 cggatgatgt gtcttggtca tccactgtta gaagatccta caccagcatc gattggtaat
    15421 tacgttgtga tcatgccaga ttataacgaa aaaatctatc aaaccattaa ggcatttttg
    15481 gaaaaaatcg aatatacagg ctttgccaat ttcgatatga agtatgatcc tagagatgga
    15541 gaatataaat tatttgaaat caatttgaga caaggtcgta gcagtttctt tgtgacattg
    15601 aacggcttga atctggctcg tttcgtcaca gaagatcgag tgtttaacaa accctttgtt
    15661 gaaacgacct atggaacgaa tcaatcagat aaagcaagat tatggatggg tgtgcccaaa
    15721 aaaatctttt tagaatatgc tcgggaaaat gaagacaaaa aactagctga gcaaatgatc
    15781 aaagaaaatc gttacggtac tactgtgttt tatgaaaaag accggtcaat caagcgctgg
    15841 ttgttgatga aatatatgtt ccataactat attccaagat tcaaaaagta ttttcatgtg
    15901 aaagaaggct agttcatgga gaattttttc agtattttag gcggaatggg cacgatggcg
    15961 acagaaagtt ttgttcgctt gatcaaccac cgaacgaaag ctacaaaaga tcaagaatat
    16021 ttgaattatg tcttattcaa tcatgcaaca gttcctgatc gtacagctta tattttagat
    16081 cgatcagaag aaaatccaat gccgtttctg ctggatgata ttgagaaaca aaatttattg
    16141 cagccgaact tcattgtgtt gacttgtaac accgcacact acttttttga agaattacaa
    16201 gcggcaacgg atattcctat ccttcatatg ccaagagaag cagcaaatga acttgtccgt
    16261 cagcatacga cagggagagt agcgattcta ggaacagaag gaagtatgaa agctggaatc
    16321 tatgaacgcg aagtcaaaaa tcttggattc gaaacagtga ttcctgatac tgttcttcaa
    16381 gaaaaaatca attatctgat ctatcatgag atcaaggaat cggattattt gaatcaagag
    16441 ctatattacg aaatcttaga agaagcggta gagcgcctaa actgtgaaaa agttatctta
    16501 ggttgcacag aattgtcatt gatgcatgaa tttgctgaag ataaccatta tccagtgatc
    16561 gatgcccaat ctattttagc agatcggacg attgaacgag cgctagctga aagaagcaag
    16621 gcattggata cagcatcgga aaagtaaaaa aattggattt ggggcaaata tcctaaaaga
    16681 tatttggtcc aaatcttttt gtaaaagaaa aagaaaactg gtaatgatac gcccggaatg
    16741 atataatttt actatgcaca atgtgagagg agttcgtaat tatgacagat aatttgcaat
    16801 tagtcatcat aacaggtatg agcggtgcag ggaaaacagt agctatccaa agttttgaag
    16861 atatgggcta tttttgtatc gataatatgc cgcctcgatt gattccaaaa ttctgggaat
    16921 taatcaaaga atcaggcaaa gtcacaaaaa tggctttagg agtggattta cgttctcgtt
    16981 ccttctttga agaaatccaa aacatgctga tcgaaatcga aaatacgaac ttcatcgaca
    17041 cttctatctt atttttagat gcttcagacg aagaactagt ggctcgctac aaagaaacaa
    17101 gacgagcaca tccaatggcg atggacggtt tgatcactga agggatacgt aaagaacggg
    17161 cgatactaga cgacttgaaa gcccaagctt ccgtgatcat tgatacgacg acattgacac
    17221 cacgacaatt acgtgaaaaa atcaatgagt cattcaaatc aaagggagac caaggatttc
    17281 ggatcgaatt tgtatcattt ggttttaaat acgggttgcc gatcgattcg gatattgtga
    17341 tggatgtgcg ttttttgccc aaccctcatt atatcccgga actaagacca ttgaccggaa
    17401 aagatcagcc agtctatgat tatgtgatgt cctttgatga aacgcaatca ttctaccata
    17461 aatttttgga tcttctggaa actgtattgc ctggatacat taaagaagga aaaagcagct
    17521 tgacagttgc aatcggctgt acaggcggtc aacaccgttc tgtggcattg actgaacgtt
    17581 tggcaaatgc tttagggaaa aactataaag tcaatgtgac acatcgggac aaggataaaa
    17641 ggaaagagac ggtgaaccgg tcgtgaaaat gaaaacctac cggataagaa aaccaaagat
    17701 cgtagtggtc ggcggtggca ctggtctgcc agttatcctg aaaagtttgc gaaatcaagg
    17761 agcagatatc acagcagtag tgactgttgc agatgatggg ggaagcagcg gtgcaatcag
    17821 agaatcgatc gctatggctc ctcctggtga cttgagaaat gtcttagtgg cgttatcgga
    17881 tatgccacaa ttctatgaag atattttcca ataccgtttc aaaaaggaag atcagttttt
    17941 agccaatcat acgatcggta atctgatcat tgccgcagtt tccgagatgc ggggaagcac
    18001 ttatgaggcg attcaattat tagcaaaaat gatgcatgta gatggacatg tttatccttc
    18061 ttcagaaact cctttgacct tgcatgctgt ctttaaagac ggaacgactg cagtaggaga
    18121 atctaaaatc gcgatcgacc gtaaaaccat tgaccgtgtg tatgtctgca acacaaatga
    18181 taaaagtgaa gccaaagcgg ctcgaaaagt cgtttcagca attatggacg ccgacatggt
    18241 cgttttggga cccggaagtt tattcaccag tatccttcca aatttagtga taccagaaat
    18301 cggcgaagca atgctcaaga caactgctga aaccgtctat atctgtaata tcatgaccca
    18361 aaaaggggaa acggaacatt tcacagatgc cgatcatata cgtgttttac acaagcattt
    18421 gaatcagcct tttatcgata cagtattggt caatacggaa aaagtgcctg aagattatat
    18481 ggacccagag atctatgatg agtatctagt acaagtcgca cacgacttca acggattgag
    18541 aaatgaagga tgtcgagtga tttccaccga ctttttggaa ttgaaagatg gtggtgtctt
    18601 ccacgatgga gacaaagtag tagaagaatt gttccgttta gttttcggtt caaaatatta
    18661 agcaatttat tgttggagaa aggaggagaa taggatgtca tttgctgcag atgtgaagaa
    18721 agaactaact ggtttggaag ttcatcgaga acatgcgaaa gcggaattag ctgctttgct
    18781 gcgaatgaac ggctctttag gtattgtgaa ccagcagttt attttgaacg tccaaacaga
    18841 aaatgctgca attgccagac gtatctattc tcttttgaaa gatcattatc aggtacgaag
    18901 tgagctactc gttcgtaaaa aaatgaaatt aaaaaagaac aatgtctata tcgtccggtt
    18961 gaagcaaggc acaaaagatg tgttgatgga tcttgatatc atggatggta tgatgttcaa
    19021 tggacacgtt tcggacgaga ttatgggaaa ttcgcaaaaa atgcgttctt acttacgtgg
    19081 tgcttttatg gcctcaggtt cagtcaataa tccggaaacg agccgttatc atttggaaat
    19141 attttctatt tatgaacagc ataaccaaga catttgcgat atgttaaatt attacggtct
    19201 acatgctcga acgcttgaac ggcgcaatgg ctatatttct tatttaaaag gagcagaaaa
    19261 aattgctgat ttcttaacat tgattggtgc aaccaactca atgctccgat ttgaagatgt
    19321 tcgaatcgta cgggatatga gaaattcggt caatcgtcta gtcaattgtg agacagcaaa
    19381 tctgaacaag acgattgatg cggcttctaa acaaatcgaa aacatcgaat tgatcgaagc
    19441 aaaggtcggt ctacatgccc ttcctgaaaa acttcaggaa atcgcagaac ttcggcttca
    19501 acatccagag gtcagcttga aagaattagg ggaaatgatt ccctcaggtg cgatatcaaa
    19561 atcgggaatt aatcaccgaa ttcgcaaaat aaatgaattt gcggaaaaac tgcgtgaaga
    19621 agcagtttaa tccaaagaaa aaactgatac atgaagcagg agatgcgaat gtatcagttt
    19681 tttttaaaag ttatacttgc ttttcttccg tcaaactata attccatctt tactggagta
    19741 acataatttg gaaaggcttc tattgttttt aatacaatcg tataaaaagc ggggagacaa
    19801 ctgaatgaag attttagttt tgtacataaa tttttgctta atatagaagt cctgatatag
    19861 ataagatcaa gtatttatct ataactggcc tgattaatta aaaaactgga aggaactgct
    19921 cgatcatcct tgagaactgc tcttcttact tctggtcaag attctcgtgg ttggtggata
    19981 aggatggttg ataagttcat tggattagct agtcgaaaac aaccaggtgt aattaatgta
    20041 gaagttttaa caaggggatg gagtgattta ttcagtgata atcataagtt cataaatagt
    20101 aattgagatg tatggttgga atacgttata cctgaacttg aactaccaag atttggttta
    20161 gaatttgaga atcgatgttt ataaagggag ggcaaaaatg cgttttttta caacttttac
    20221 tatcattatg attgcagtac tttttatttt tctagatata gcaaagcgaa atacagcatt
    20281 tttattatat cgggttttac ttcgagcagg tctcataaca tttatatcga tcgttggatt
    20341 ttttctattc acagttattg tatttatttg gagaactcca gcaccaccat tgccagagat
    20401 tacatatgga gaatttcctt ttagacttga gtatgaattg aatgaagaat tacatgtgat
    20461 agaagacacg ttaatagttg aatttgatgg atttggaatg aacgaaggca tagggagata
    20521 tcgtagatgg acaagtcgtt tagccagcgg agaagattta gtgttgctgt tagaagttag
    20581 tgataataag caaatatttt attttccagg acctgctaat tattacatgg gagataggtt
    20641 gaatggttat aatcatactt ttcctagtgc ttctttcatt gaaagggaaa gaggcattac
    20701 tagaagggat attttacatg acaaagaatt attagaacaa tttggtccgc ttgatcaaaa
    20761 tacaataaat gaagaagaat tgttaaatca atataatata aggctagtga attgggaaat
    20821 atctgaaccg attgttaaca attttggtga ttagaaaaca attacgataa atagaaacaa
    20881 gccaaatcat cagcaagtaa aagctgatga tttggccttt cttatacgag tagtaggaat
    20941 tcatagcgaa tattattacg atcagtagat attttgatat taaaggagta ttacgaacta
    21001 gtatgtgaga atgtagaggt gatttgcgaa taatgagaaa tttatcaaat agaaacaaaa
    21061 tattaatcat tattgttgtt atagctgtgt ttcatctggg aacgaatgcg gttttaagta
    21121 gaataatatt gggtcctaag ccaccaagac ctgcgattac ccgaggggag tttgatttta
    21181 gattagaata tgaagtagat ggggaaagga ttgtgataga agatacaatt gttgcattat
    21241 ttgatggatt ttctgcagat gcaggtagca tggcatggta tcgcacgtgg cgtttgcatt
    21301 tggcgagtga caagagggca caacaattat tgttacatga gttagatgat ggtcgaaaaa
    21361 tatattatca ttttggagga gctggatttt atatggggaa gttagtagca gtgtaactgt
    21421 aacaaggaaa gatttgccag gtcttggcat tcgaaactcc atattttata tcgagcgtgg
    21481 gagagataca tctattggga acaggaaagg gttaactctt gaagagttat ataatgactt
    21541 tggaatccgt cttgttagtt gggaacacaa tccaccaata gaaaatcgct ttgaataatc
    21601 gaaatctatt aatccatttc atcagccagt gaatgttgta atggaacaat ttggtttagt
    21661 gttcaaaacc attgaaatac taggctgtga tcagcaaaaa tgtatctgaa taaaaaagag
    21721 agcaactatt aaatgagagg agttaggagt tgttagcttt atacgaatca aagtggcaaa
    21781 tacagatgcg tatgcatttg tgtttgccat cttgcgtttg gtagacgaga taggtataag
    21841 ctaaagaaac aacttgttta aaataaacga cctagctcaa caaacgacca tctaatcaac
    21901 taacagcata cataaaagca actatttttg aagtagaatt gaaatcggga taacttaaaa
    21961 agtgattggg atactagtgt gaatcaccgt acataaatat tactcgttaa gggaatttga
    22021 gaaattttgt atgaaaattt aagaattagt tagtatgcgc aagtcaaaaa tatgaataaa
    22081 ttatttggtg gagataattt gctcagcttg tcataagaaa tgagataatc tggcagatgc
    22141 aagcttttgg acaccattta ttcggaataa ggaggctggg acagaagcgt ttaactccaa
    22201 gaaataagaa ggagttgctt ctgcttccgc cgtttatagg tgtttagagc gtgagacaaa
    22261 agtgttcttt acttttgtcc tacgctcctt atttttttat tttgtttgga tcaattcttt
    22321 taattcctct attgaaatag attcatcaaa gatttcttta cccaagatga cagtcggaac
    22381 aaatcgaata tgtgcttgct cagcttcgtt gatgacagct tggctcagct gttcgtcttt
    22441 ttgttctgtc agctttagtt tatctactgc aaaatccgct acttcttgga gagaaagatg
    22501 tttccactcg tcttgagtat cgaatatttg tttgatctct ttgatcgctt tttgaccatc
    22561 gctgattgtg agataccggt gcatcacgtt tcctcgctgc aagctttctt tttctttatc
    22621 gaacaatttg atcactcgct gcaattgccc gctttggact gcttcttcta atagctcata
    22681 agattcttca aaccattgtc ggcaatacgg acaagatagg ttgatgaatt cgaccatttt
    22741 tttaggagca ttcgatgaac cataaaggat tcctttttgt gtatttgttt tcgttgcatc
    22801 aatcactgat atatccatgt taaaacacct ctttcgctta tagtttatac atgtctgaca
    22861 aaaaaaggca acgatttaga ctgtttgtct aaaaattaga tgaccaattc aaatagacat
    22921 atgtgaaata tttttataaa taaaagaaaa aatggaatgt tttacgttat aatggaacag
    22981 ataataagag agaatatgaa caaggatgat gaatcatgcg aaaaatgaaa acaatggatg
    23041 gcaatacggc agccgcatat atttcttatg catttaccga gattgctgca atatatccaa
    23101 taacacccag ttcgaccatg gctgaattag tagatcaatg gtcagcagca ggaaaagaaa
    23161 atgtattcgg tcaaccagta aaagtagtag aaatgcaatc cgaagcagga gcagctggtg
    23221 tggtccatgg atcattgaaa actggtgcac tgacaactac atataccgct tctcaagggc
    23281 tgcttttgat gataccgaat atgtacaaga ttgctgggga actactgcca agtgttttcc
    23341 atgttgctag tcgggcagtg acgacaaatg cgttgaatat tttcggagat cacggagacg
    23401 tgatggctgc aagacaaaca ggatttgcta tgttagctga aagcagtgta caagaagtga
    23461 tggatttagc acctgttgcc catcttgctt ctattgaagg agaagtacct tttttgaatt
    23521 tctttgatgg attccgtacg agtcacgaga ttcaaaaaat cgaagttttg gaatatgaag
    23581 aattagctcc tctgatcaat caggagaaat tagcttcatt tcgtaaacga gggatgaatc
    23641 ccaatcatcc gactgttagt ggtacgaacc agaatccaga catccatttt caacaacggg
    23701 aaacgatcaa cccctattat gataaattac ctgaaatcgt tcaaaaatat atgaaagaga
    23761 tcaaccgatt acgaggaact tcttatgact tggtgactta ttacggagca gacgatgcgg
    23821 aagaagtcat tgtttctatg ggatcagttg cacagacgat tgaacaaacg attgattact
    23881 tgaatgcaaa cggacggaaa acaggttttt tgaatatcca tctttatcgt ccttttccag
    23941 tggaaacatt cgtgcaaaag ttgcctcata cagtaaaaac aattgctgtt cttgatcgaa
    24001 gtaaagaacc aggggcaggt ggagaacctc tcttattaga cgttcaaagt gcgttatatg
    24061 attctgaact tcgcccggct gttattggtg gtcgctacgg attggggtct aaagatgtga
    24121 ctcccgacca aatctcagca gtttttgatg aattgaaaaa agacccaagc atacgcaaaa
    24181 aacggtttac catagggatc gtggatgatg tgacttatca atctttggaa ccaaaggaaa
    24241 gtctggattt gacagaaccg caaacattcc aggccaagtt ttggggattt ggatctgacg
    24301 gaacagttgg agcaaataaa tcagctatca agattattgg agatcacacc gataaatatg
    24361 cgcaaggcta tttttattat gattcgaaaa aatctggcgg tctgactgtc tctcatcttc
    24421 gttttggaga tacgccgata cgttcagcgt atcttgtcga gcatgcggac cttgttgcct
    24481 gtcacacacc agcttacctg catagctatg acttggtcaa aggattgaag ccaggtggca
    24541 tttttttatt gaatactctt tggagcgacg aacaattaga aacgcatctg cccttaaaat
    24601 taaaacgtta cttggctgaa aataacatta gattctatac gatcaatgcc atgagattgg
    24661 cccaagaagt cggtttggga cgccggatca atacggcaat ggaaacggct ttttttaaat
    24721 tagctgacat cattcctttt gatgaggtgc ttccgctgct aaaagaagaa gccctgaaaa
    24781 gctatggaca taaatcaatg gctatcgtag aaaaaaacat acaagcaatc gatcggacga
    24841 ccgaactgct tcatcaagtc gcgatcccag accattggag aacgttggag atgcctgaaa
    24901 aaatcaataa aaggaacact ggttatatac aagaaatact tgaaccagtg aatgcacagg
    24961 aaggaaatca actctctgtt ggtacacttg tagcaaatgg aatgacaact ggcgaaatgc
    25021 ctttaggaac ggcttctatg gaaaagcggg gcgtcgcttt agaagtacct gagtggatta
    25081 gtgatcgctg tacgatgtgt aatgaatgtg cctttgtttg tcctcatgct gccatccgtc
    25141 catttttagc agatgaagag gaaatggaag aagcgccaga aggatttatc gtgagagaaa
    25201 tgcgaggcgc agacggcgta aaataccgta tccaagtttc agtggaagac tgtacaggtt
    25261 gtggtctgtg tgtagaggct tgtcccgcaa aagaaaaagc tttagtgatg aagccttatg
    25321 aagaacagaa agaacaagct gtgaattggg cttttgcaat gacattaaga caaaaagcaa
    25381 atccagtcaa aggaaagaat actgtcttgt ctacacaatt caatcaaccg cttcttgaat
    25441 tctcgggcgc ttgctcaggc tgtggcgaaa caccttatgt aaaattattg acacaaatgt
    25501 tcggtgatcg aatgatgatc gcgaatgcga caggctgttc ttctatttgg ggcggtgctt
    25561 ctcctgcttc tccttatacg accaatgaat gtggtcaagg gcctgcatgg tcaaattctt
    25621 tattggaaga taatgctgaa tttggttatg gtatgcttct agcggcacag actcgtcgcg
    25681 aggctttggc aagttcaatc aaagaagtat tgccaaaagt atcagaagaa ctacgagaat
    25741 tgatggaaga ctggttggta cacatgtttg agagcgaagg aacgcagcaa agagcagcaa
    25801 aactgcaagc tgcgatcgaa agcgaacgat caattgtccc tgaactggag gatatataca
    25861 agagaaagga tctattcgta aaaaacagcc aatggatgat tggtggagac ggatgggctt
    25921 acgacattgg ttttggcggg atcgatcatg tacttgccag cggagcagac gtgaatattc
    25981 ttgttttgga taacgaagtc tattccaata ctggcggtca aacctcaaaa gcaacaccag
    26041 cttcggcaat cgctaaattt tcggctagtg gaaagtatgc ttcaaagaaa gatctaggaa
    26101 tgatggcgat gacatatgga aatgtttatg tggcgcaaat tgcttcaggt gccaatcaaa
    26161 tgcagacgat aaaggctttt gaagaagcag aaaagtatcc tggaccttct ttaatcattg
    26221 cctatacgcc ttgtatcaca catggtttgc ttggtggaat gaaagaatcg cttaaagaag
    26281 caaaagaagc tgtttcttct ggctattggt cattatatcg atataaccct gcaatggtcg
    26341 aaaaaaacaa gaacccaatg actttggact acaaaaaacc tgatttctct gctatgcctg
    26401 atttcatgag gaaacaagtt cgcttttcct cgctagagaa cgcaaatcca aatcttgcaa
    26461 aagctttatt tgaaaagact atccaagacg ctaaaacacg tttttacaac tatgctgcac
    26521 tgacgggaga attagaaaaa atcaagaaac gacttgaacc agaggaatct atcgatgtac
    26581 ccgttaagac tagaaaagaa aaaacgacgg accttgaagc agaagaaaga agagctgcca
    26641 gagcagaacg acgtaaaaaa atggaacaag ctacaaaaga aaaaaaggaa aaataacaga
    26701 aaaaatataa tgaataattc tctgaagttt aaaaatattt attgcagaga aaaataaaac
    26761 gcatacttgc aattttcctg aaaattatgc gtttttctca agaaaaatac gagcagatca
    26821 gcctctaaaa ttaaaacata tgatatacta gagaggaaat ctcaaaagag aagagaggct
    26881 ttttatgtca tttcaaatcg gggaactttt taatttggac tattggcagc aaatgttttc
    26941 cggtgacttg ttctctctga attttttggt gaacatactt gatattctgg ttgtctggta
    27001 tcttgtatac aaactgatcc aactgctgcg aggcacaaaa gcgattcagc ttctgaaagg
    27061 tgtagctatt tttattgtta tccgaatcgt agcggaaatc attggtctgc acaccttgtc
    27121 ttggctgatg aaccaagtca ttacgtacgg ggtgatagct gctatcgtga tttttcagcc
    27181 ggaaatccgg agaggactgg aacatcttgg acgcagttcg cttttcaaac aatcaaaaag
    27241 cgaacagcag gaagatgaga agatgatctt gtcttttgac aaagccatcc aatatatgtc
    27301 caagcgaaaa atcggtgcgc taattactat cgaacggcat actggattag atgagtatat
    27361 tgagacagga attgcattag atgcagatat cactggagag ctgctgatca atatctttat
    27421 tcctaataca ccgctgcatg acggtgcagt tattgtaaaa gaaggaaaga tcgcagtagc
    27481 aagcgcctat cttcctctat cagaaagtat gttgattcca aaagaattcg ggactcgaca
    27541 ccgggcagct gtcggtataa gcgaagtcag cgacgcgatt accatcgttg tctctgaaga
    27601 aactggggat gtcagcatta cattagataa tgaactgatg gctggcttat cccagcaaga
    27661 atatttggca attttgcgta gagaactgat tgctgaagag aaaacaaaaa acaagaaaaa
    27721 cgtgattcaa tctttcctag acggtatgac taagggaggg gatcgcaaat gatctcaaaa
    27781 gagaaacgga caagcattct ttacagtttt attgcattgc tgttcagtat tgcgctgtac
    27841 ttcaatgcaa atggccagtc cgttccaaat acgctttctg gaaatgaagc gtataatcaa
    27901 acagttactg gggtgccaat caagatttct tatgattcaa agcgctacta tatccatgga
    27961 tatgaaaatg cagtgactgt caaactttcc agtgcaaacc gagttcagtt gaatgcagag
    28021 gcaaacgaag atacacggat gttccgcgtc acagcagata tcactaagct gggcgaagga
    28081 acacatgagg ttccgctaaa aattcaaaat ttgagcagtg ctgtcacagc aaagatcgaa
    28141 ccatcgacta ttacagtaac ggttgagaaa aaggtaataa aagatttcga tgtggaaacg
    28201 ttgattcctt caacgttcac accatctgga tatgaacttg atgatacttc cgttagtcct
    28261 aaaaaagtct ctatcactac aggtgacaaa acattggcag aaatcaaacg agtagtagct
    28321 gatgtggacg cttcgatggt cacagatgat gggattaata gtgaggtccc aatacaggcg
    28381 ctgaatgcag caggggaagt attaagtatc gtctctgatc cagtgcaagt aacagtgaga
    28441 gctgattcga tcaagccaag caagtcggta cgactttatg ggatacagca aggaacacca
    28501 gctgccgggg tagaaagcta tgatttcagt ttttcaaaac tggaagcaga agtaagcggc
    28561 tctagcgatt tgcttgcatc gatcggtgac agtattgctg taccgatcaa tgtctcaggt
    28621 atcaatcatc gaacaactcg taaaattgaa ataccagtag aagaggggtt aagtatcaag
    28681 cccgaatcag ttagtgtgga gattacaccc gtgttgcaaa cgtcaagcag tagctctgct
    28741 tcgacgagca gtcggacaca agaaaataca agtacgagta gaagtatttc atctgctgat
    28801 tcaggaacgg aaccatcgac aacaaccatt tcttccagtt cggaatctgg gacaagcagc
    28861 tcgaccacaa gtacagaaga aacatcagaa acaagcgaat caacacaaat gagcagaaca
    28921 aattcctcag aaaattaagt taaaggagta attttacaac tatgggtaaa tattttggaa
    28981 cagacggcgt acgcggtgaa gccaacaagg aacttacgcc agaacttgca tttaagctgg
    29041 gacggtgcgg aggttatgtc ctaagccagc atgaagaaga aggaaaacgc ccacgcgtgt
    29101 tagtagggcg ggatacaaga atctcaggtc aaatgttaga agaagcgctg atttcaggac
    29161 ttttatcggt gggtatcgaa gtgttccagc taggtgtgat ctctacacca ggtgttgctt
    29221 atctgacacg tttgcaaaaa gcaagcgcag gggtcatgat ttctgcttct cataatccag
    29281 cacaagataa tggaatcaaa tttttcggtg cagatggttt taaactagta gatgaccaag
    29341 agctagaaat tgaagcatta ttagatgctg aagtggatga attgcctcgt ccatcagctg
    29401 aaggattggg aactgtagaa gaatttccag aaggcttatt gaaatattct caattccttg
    29461 tacaaacgat caacggtgac ctttccggtt tgaccatttg tgtcgatgca gcaaacggag
    29521 caacttcaac aagtgttaat cgtttgtttg ccgatcttga aacagatttt tataccatgg
    29581 gcacaaatcc agacgggtta aatatcaatg atggcgtagg ctctacccat ccagaacgtt
    29641 tagctgagat ggtcgttgaa aaaggagcag atgctggact agctttcgat ggcgatggcg
    29701 atcgtattat tgcggtggat gaacttggaa acattgtcga cggtgataaa atcatgttta
    29761 tctgtgctaa atacttggca gaaaaaaatc gattgaaaaa agatacgatc gttacgactg
    29821 tcatgagtaa cttaggattc cacaaagcgg tggaaggaat cggtttgaaa gatgtcgtta
    29881 cacaagttgg tgatcgttac gttgtagaag aaatgcgcaa aaacgattat aactttggcg
    29941 gcgagcagtc tgggcacatg attttcttag attttaacac aactggagat gggatgcttt
    30001 caggaattca gttactgaat atcatgaaac agacaggtaa gaaattatca gagcttgcag
    30061 cagaggtaac aatctatccg caaaaattag tgaatatccg agtaagtgat aaaaacggtg
    30121 cgatggatgt tccggcaatc aaaaaagtga ttgaagaggc agaagctgaa atgggatcag
    30181 aaggaagaat cctagttcgt ccttccggta cagaaccatt acttcgagtg atggcagaag
    30241 cgcctactga tgagaaagtt aattactatg tcgataaaat cgcggcagta gtcaaagaag
    30301 aaatcggact ggattaagag atattttca
//
LOCUS       DACDPS010000028        29104 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.028, whole genome shotgun sequence.
ACCESSION   DACDPS010000028 DACDPS010000000
VERSION     DACDPS010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.028"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..1031)
                     /locus_tag="IWJ07_001333"
     CDS             complement(<1..1031)
                     /locus_tag="IWJ07_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9332922.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1113..1802)
                     /locus_tag="IWJ07_001334"
     CDS             complement(1113..1802)
                     /locus_tag="IWJ07_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP9332923.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="IWJ07_001335"
     CDS             complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="IWJ07_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAP9332924.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2329..2832)
                     /locus_tag="IWJ07_001336"
     CDS             complement(2329..2832)
                     /locus_tag="IWJ07_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAP9332925.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="IWJ07_001337"
     CDS             complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="IWJ07_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAP9332926.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="IWJ07_001338"
     CDS             complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="IWJ07_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAP9332927.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4800..5468
                     /gene="sdaAB"
                     /locus_tag="IWJ07_001339"
     CDS             4800..5468
                     /gene="sdaAB"
                     /locus_tag="IWJ07_001339"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAP9332928.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5481..6353
                     /gene="sdaAA"
                     /locus_tag="IWJ07_001340"
     CDS             5481..6353
                     /gene="sdaAA"
                     /locus_tag="IWJ07_001340"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAP9332929.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6444..7463)
                     /locus_tag="IWJ07_001341"
     CDS             complement(6444..7463)
                     /locus_tag="IWJ07_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP9332930.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7636..8523)
                     /locus_tag="IWJ07_001342"
     CDS             complement(7636..8523)
                     /locus_tag="IWJ07_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9332931.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="IWJ07_001343"
     CDS             complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="IWJ07_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAP9332932.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9246..9416)
                     /gene="secE"
                     /locus_tag="IWJ07_001344"
     CDS             complement(9246..9416)
                     /gene="secE"
                     /locus_tag="IWJ07_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAP9332933.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="IWJ07_001345"
     CDS             complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="IWJ07_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP9332934.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9754..10398
                     /locus_tag="IWJ07_001346"
     CDS             9754..10398
                     /locus_tag="IWJ07_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9332935.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10454..10957)
                     /locus_tag="IWJ07_001347"
     CDS             complement(10454..10957)
                     /locus_tag="IWJ07_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP9332936.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10970..11016)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11554..12519
                     /locus_tag="IWJ07_001348"
     CDS             11554..12519
                     /locus_tag="IWJ07_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP9332937.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12618..13790)
                     /locus_tag="IWJ07_001349"
     CDS             complement(12618..13790)
                     /locus_tag="IWJ07_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9332938.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13777..14922)
                     /locus_tag="IWJ07_001350"
     CDS             complement(13777..14922)
                     /locus_tag="IWJ07_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9332939.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14936..15868)
                     /locus_tag="IWJ07_001351"
     CDS             complement(14936..15868)
                     /locus_tag="IWJ07_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9332940.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15985..17064
                     /locus_tag="IWJ07_001352"
     CDS             15985..17064
                     /locus_tag="IWJ07_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP9332941.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17061..17690
                     /locus_tag="IWJ07_001353"
     CDS             17061..17690
                     /locus_tag="IWJ07_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9332942.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17800..18027)
                     /locus_tag="IWJ07_001354"
     CDS             complement(17800..18027)
                     /locus_tag="IWJ07_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9332943.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18130..20718)
                     /locus_tag="IWJ07_001355"
     CDS             complement(18130..20718)
                     /locus_tag="IWJ07_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAP9332944.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20738..21397)
                     /locus_tag="IWJ07_001356"
     CDS             complement(20738..21397)
                     /locus_tag="IWJ07_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9332945.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21446..21949)
                     /gene="trmL"
                     /locus_tag="IWJ07_001357"
     CDS             complement(21446..21949)
                     /gene="trmL"
                     /locus_tag="IWJ07_001357"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAP9332946.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22170..23036)
                     /locus_tag="IWJ07_001358"
     CDS             complement(22170..23036)
                     /locus_tag="IWJ07_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP9332947.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23145..23786
                     /locus_tag="IWJ07_001359"
     CDS             23145..23786
                     /locus_tag="IWJ07_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAP9332948.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23848..25209)
                     /gene="mgtE"
                     /locus_tag="IWJ07_001360"
     CDS             complement(23848..25209)
                     /gene="mgtE"
                     /locus_tag="IWJ07_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAP9332949.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25227..26126)
                     /locus_tag="IWJ07_001361"
     CDS             complement(25227..26126)
                     /locus_tag="IWJ07_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP9332950.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26128..26925)
                     /locus_tag="IWJ07_001362"
     CDS             complement(26128..26925)
                     /locus_tag="IWJ07_001362"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAP9332951.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26903..27607)
                     /locus_tag="IWJ07_001363"
     CDS             complement(26903..27607)
                     /locus_tag="IWJ07_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAP9332952.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27738..28313
                     /locus_tag="IWJ07_001364"
     CDS             27738..28313
                     /locus_tag="IWJ07_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAP9332953.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28431..>29104
                     /locus_tag="IWJ07_001365"
     CDS             28431..>29104
                     /locus_tag="IWJ07_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP9332954.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLS"
BASE COUNT         8562 a         6169 c         5232 g         9141 t
ORIGIN      
        1 aatacagtat tatttcggtt gcttgctgct tcacctagcc cttgtactcg tgtttgattg
       61 gcgacttgac caaattgatt cccgacgtct aataaaataa tagctaaaag caaaatggcc
      121 aaatggcttc cgccaaccaa taaagcaata aagcttattg tttgcataac aagtcccata
      181 tacacgattt ttctttcaga ataagaatct gccagtcgtc ccacgatagg tgcagcaact
      241 gctccagaca aaccaaaaat cgctaatatc cctgcttcga atgttcccca attgtaggcg
      301 ggactgctga tataaaaaat caacgtagac caaaagatcg aaaatgttcc aaacatgaaa
      361 aatccactga ctgccgcttc tcttaataga cgttgagaag caactaaatg cggcaaactt
      421 ttaagtgaat caaggtatga aacagctgta ctactttgct ggacagaacg cggcattttc
      481 cagtaaagca gaagtgtaag aattccgact gcacataaag caaataaata gatcatccgc
      541 caagaaaaaa gactggcaac aataccagat accgtccgag aaagcaaaat ccccacgagt
      601 aaaccgctaa gcaatgtacc catgaccttc cctcttgctt ttggtcccgc tagtaccgca
      661 ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat
      721 gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc agataacgct
      781 accattcgaa tgatcaattt tggacgatcc accacgtccc ctaaaggaac taaaaacagt
      841 aaccctaacg catagcctaa ttgggtcaac atcgtcaata tcccgactgc acttgtgcta
      901 acagataaac tagctgctac ttttgttcca atcggctgga tgtaatacat attggcaaca
      961 acaacgccac aagtgattga taatagtaaa ataagcgaag ttgttaaagg tgttttttca
     1021 ttactcttca ttgatttccc ccatttccta tcttagttta caacttatgg aaaagattgg
     1081 aaatagaaaa atacaactat tcgccacact tattatttgt gcttttgttt tccttgtttt
     1141 ttcttgcgat ctttataaat gaagacaact aaccctacta gcacagtaac acttgcaacc
     1201 aacgtgactg cagttttgtt atatccgtga aacagagctt ctccaccaca cgcatacaac
     1261 acagacgaag ggatgactcc aatcacgatc gacaatatga tttgctgtag aggcaatttc
     1321 atcgcatctg cagcaaaatt gaccatagaa gatggaataa ccggtatcat atagcctaac
     1381 attacaccta tttttggatg tttcatttgt cggattgctt gcacccaatg attcgtttcg
     1441 gttttcttat ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga
     1501 gcattaccca aaatgttgat gatcgcacct gtcagtggtc caaagctcaa accagcaatg
     1561 actccaacta ctgaagtagg aatacctgga acagcacaca tcaccgctgt caatacgatc
     1621 aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc tgccttactc
     1681 gctctcggat ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa ccgatagccc
     1741 actaccagta tcagaatgat tcccaacgag atcaacatca atcggattat tttctttttc
     1801 atatttcctc ctgaaaaaaa tctatattca ttaaataata gtttaacata aaaacaaaaa
     1861 agcaccgaaa tccaacatag gatctcagtg cttttaaata gtaaaaatta tttaacagtt
     1921 acagatgcgc caacttcttc aagtttagct ttcaattctt cagcttcttc tttagatacg
     1981 ccttctttaa ctggtgcagg agcgccatca actacagctt tagcttcttt caagcctaaa
     2041 ccagttgctt cacgaactgc tttgataact ttaacttttt ggtctccagc tgcagttaat
     2101 tctacagtga attctgtttg ttcttcagca gcagcagcgc caccagcagc agctacagct
     2161 acaggagcag cagcagatac gccaaattct tcttcaatag ctttaactag gtcgttaagt
     2221 tctaggatag ttgcttcttt taactcagca acgatgtttt caatgttcaa tgccattata
     2281 atttcctccg tttaattgtt cttttttatt taatgcttta tgcggcaatt aagctgcatc
     2341 tccatcttct ttgtctgcca ctgctttgac agcgtaagcc acgttgcgga ctggcgcttg
     2401 tagtacagat agcaacatag atagaagtcc ttcgcgattt ggcaattttg ccaatgctgt
     2461 gatttcttcc aatgaagaga ctttaccttc gatgataccg cctttgattt ctaatgcttt
     2521 tgcttcttta gcaaattcgt ccatgatttt cgctggtgcg actacatctt cgttactgaa
     2581 tgcaacagct gtaggtccag tgaatacttc gtccatgcct tcaaggccag cttttttagc
     2641 tgcacgtgaa agaatagaat ttttgataac tttcatttct acgttagcat cacgaagttg
     2701 tttacgtaaa cgtgttactt catcaactgt taatccgcgg tagtcaacga ctactactga
     2761 tgcagctgct gtaaattttt cgcttacttc atcaacgatt gctgcttttt ttgcgataat
     2821 tgcttcactc atttttgagt ttcacctcct gaattttgat ggaagagttt tttgcactga
     2881 aaaggcaagt cacttttata gttactttgc tcaaccagta ttattcatca cctattcact
     2941 tcgttcatag tgattctact cgctttgctc gaaaggaaac tcctttcgct ttgctcaacc
     3001 agtattgttc atcatctctt cacttcgttc atagtgattc acaataaaaa actctatgcc
     3061 accgcagaca tagagggact attgaatata ttcaataaac gtcctcggta ggaaattaag
     3121 gcttgatgcc acctactgtc ttcggtacaa gttaattatt aacctcaact agcttagcat
     3181 gctaaactag ttgagtcaag aattattttt cagttggatc aatcaacatt ctaatattta
     3241 attagaaaga agcttggtca acatggatac caggtccaaa tgttgttgtg atcgttacat
     3301 ttttcatgta ttgaccttta gctgctgatg gtttagcttt tagcaataca tcatggattg
     3361 ctgcaaagtt ttcaactaat ttttcatcgt caaatgaaac ttttccgatt ggtacatgga
     3421 tatttccagc tttgtccaca cggtaagtta ctttaccagc tttcacttcg tttactgctt
     3481 ttgttacgtc cattgtaact gtaccagttt tagggtttgg cattaagcct tttggtccta
     3541 atactcgtcc tagacgacct acagtagcca tcatatcagg tgttgcaaca actacatcaa
     3601 agtcaaacca tccgttttgg attttttgaa ccatgtcgtc gtcacctacg aaatctgcac
     3661 cagctgcttc agcttctttt gctttgtctc ctttagcaaa aactaaaaca gtttgtgttt
     3721 taccagttcc gtttggtaat acaacagcac cacggatttg ttggtctgct tttttagggt
     3781 ctacgttcaa acggtatgca acttcaactg ttgcatcaaa ttttgcaatg tttgtttctt
     3841 ttgctaaagc tactgcttcg gcaacaggat aaactttact tgaatcaact ttttttaatg
     3901 catcttgcat ttttttgctc tttttagcca ttttggttcc tccttgattg tggttataac
     3961 ggttgtacct cccacgtgtt ctatatcttt caatatagag ccaactgaga agctgctgtc
     4021 ttagggtgta tagattattc tacagtgatc cccatgcttc gtgcagttcc ttcaaccatg
     4081 cgcattgctg cttcaacgtt agctgcgttt aggtcttcca tttttaattc agcgatttct
     4141 cttacttgat cgctagaaac tttcgcaact ttatttttgt ttggttcgcc tgaacctttt
     4201 tcgattttag ctgctttttt caatagaaca gcagctggcg gtgtttttgt aacgaatgtg
     4261 aatgaacggt cttcgtatac agaaataaca accggaatga tcaaacctgc ttgatcagct
     4321 gtacgagcgt tgaattcttt tgtgaatccc atgatattga tacccgcttg acctagtgca
     4381 ggacctactg gtggagctgg agttgcttta cctgcaggaa tttgcaattt aacgattttt
     4441 tctactttct ttgccacgag acatacctcc ttaagtccgt gatgtggtta attggagatg
     4501 catatttctc ctcccacgtt ttatctgatc tgaaaaaatc cagacaaata atgtgcaaaa
     4561 caatgcacac tggtacagta tagcaaattt atttgttttt tcaagatttt tttctgctat
     4621 ttttacattt tgcttgaata aacgctaaat ttattctaaa aaaacaacaa tttttcgttc
     4681 tcatttcttc ttttatgtta cacttgcgct agcatggttc aacagatgaa agcaaacaat
     4741 aacacggttt tttagaccct tgtacaaaga ttcctacata acaaaatgag gtggagaaaa
     4801 tgaaagattt aaggtataga agtgtctttg atattattgg acctgtgatg attggtccaa
     4861 gtagctctca cactgccgga gctgctagaa tcgggaaaat catacgtagt atatttggcg
     4921 aacagcctga ttctgtagat atttatctat atgaatcatt tgcaaaaacc tatcgggggc
     4981 acggaacaga tattgcatta gttggcggcc tgttagggat ggaacccgat gatgaacgtt
     5041 tagctgattc tttgaaattg gcatatgaag ccggtatgga agtcttgttt gttcctaaga
     5101 gtgaaaaagc cgaacatcca aactctgtaa agatgcttct aaaaaaagga acacgaaagc
     5161 tttcagtcac tggcatttcg atcggcggtg gcaatatcca gatttctgaa ttgaatggat
     5221 tcaaaatttc tttaagcatg ggtactccta ccttgatcgt tgtccaccaa gatgttccag
     5281 gtatgattgc taaagtgact agtatacttt ctgagaccaa tacgaatatc ggcactatga
     5341 cagtgacacg ggaatcaaaa ggtgaaaatg ccattatgat aattgaagta gatgatcctc
     5401 aagtagaaga aacggtaaaa agactcaaac agctgccaaa tatcgacagc gtaaattatt
     5461 tcgattaagg agctaaaaaa atgtttttat cgattgaaga attagtagat caagcaaaag
     5521 cttatccaag tgtagcagaa ttgatgattg cagttgaaat ggaaatgagt ggccgttccc
     5581 gtagtcacat catcgagatc atggaaaaaa acctctcagt aatggaacaa tcaatcaacg
     5641 agggaaaaga aggtgttacc tccgtcacag gaattaccgg tggtgatgca aaaaaattag
     5701 atgattattt aaatgaggca gactttttaa gtggagaaac aatcttaaaa gctgtacgca
     5761 atgctgtagc tgttaatgaa gtcaatgcaa aaatggggtt gatttgtgct acaccgactg
     5821 ccggaagcgc gggtgttgtt cctggtgtgc tgatggctgt ccgagatcgg cttgatttga
     5881 cacatgaaca gcaattggaa ttcttattta ccgctggcgc tttcgggtta gtcatcgcaa
     5941 acaacgcttc tatcagtggt gcagaagggg gatgtcaagc agaagttggt tctgcaagtg
     6001 caatggcagc agcagctttg gtttgtgcaa aaaatgggac tcctcatcaa gcagcacaag
     6061 cagtagccat tacattgaaa aatatgatgg gactgatttg cgatcccgtt gcaggcttag
     6121 ttgaagtccc gtgtgtcaaa cgtaatgctt taggctcttc tcaagctttt atctctgctg
     6181 atatggcatt agcgggtatc gaaagtgtca ttccgccgga tgaagtcgtt gctgcaatgt
     6241 accaagtcgg acgtcaaatg ccgtcgattt tcaaagaaac agcagaaggt ggcttagctg
     6301 ttacgccaac tgctaaacga cttgcggaag agattttaaa taagagggcg tgataaagct
     6361 ccttaatttc gagtattaag agattagtta tcttcataac aaaaaacgaa gagaggacta
     6421 attgcccttc tcttcgtttt ttattaatcc atcaatttcg tattttttac ttcatcgatc
     6481 gtttttgttc ctgcaagttg catcgtgatc gacaattctt tgttcaggtg ttcaaaaaca
     6541 gatgttactc cttctgcccc acctagattc aacccataaa tcactggtcg gccaatcgca
     6601 actaagtcag caccacttgc cagagctttg aagacatgct ctcctcgacg gacaccagag
     6661 tcgaaaatca ctggtacacg tttgttgact acttcggcaa tttttggcaa tacttcaaag
     6721 gacgcaggac ctccatctaa ttgacgtcca ccatgattgg atacccagat accatctgcc
     6781 cctgcagaga tagcgattac tgcatcttct ggcgattgta ttccttttac gataactgga
     6841 agatgggtga tttctttgat tgttttgatg tcatctggtg tcaagccttg ttttgcagct
     6901 gcataaattt ctgcgattcc ttttccttcg ccattaccac ttgcactttg ttcgctatac
     6961 gcagctaagt taggcattgg taatggaaat tggaattgat tgatgacgtc ttcttcacgg
     7021 taaccaccga gcgttgaatc tgccgttaaa atgatagctt tagcccctgc ttctactgct
     7081 ttgttcaaaa tgaactcatt gaatctgtcg tctttactca tatataattg gaaaaactgc
     7141 ggtgcgcctg gcgctgcatc ggctgcgtct ttgattgtcg tatttgcata agtgctgatg
     7201 gaaaagattg atccagcagc agctactcct ttagctgtat cagcttcccc tttttcatgt
     7261 gccaatcctt gggccgctga tggcgcttgg ataatcggtg tatctaattc aattccgaaa
     7321 acagatgtat gcaaatcggc tgagtcgatt ccgcgtaata tccgtggcat gattttcttt
     7381 gtattaaaag aagtcgtatt ttctttcatc gtccactcat cttcagaacc accgcgaata
     7441 tagccaaaag ctcctgcttc catgcgctct tttacacgac cttctagtta ggcaatgttt
     7501 acaatatcga ttggatgttc ttcattactt gtatgataag tcatagtaga tccttctttc
     7561 atcttcttga ttacatataa atcatactaa acttacaaaa agtaagcaaa catttactct
     7621 cagatacttt tatcgctatt ctttccaaat aaatcgactt acaagctgat ctacttcttg
     7681 gttttcatga agcatactat gagaggcatt ttcacctgtc actacttttt cctctatttc
     7741 atttccgcgt tgcttcaaca acgcatagac cgctaatgca ctgtttaacg gtactgttcc
     7801 atcactcaag tctgtttcat ctagttgacc agcaatcaag aaaaatcgag tggttatagg
     7861 aatattaccg atcatttgct ggtaatcttg atagcggtta gaaacgacag caggcccctt
     7921 tgctaactca tctgtcagag actgcgcact atcatcgaca aaatcattaa aaggcgcgcc
     7981 aattgctgtt aatgtctgaa cttgtggcaa tgacgtatcc tgtccatacg ttcctaaata
     8041 gcgtagtgca ctaactcctc ccatggaatg ccccactatg ttgactttat ctatgccgta
     8101 gttttctttc agatacaaaa gacaagtttt gatccattct gcttgattcc actcgttatt
     8161 tttattatct tcaaacaata cttggaccat cggattgtct gcttttccag acaaatctcc
     8221 tgttgcttgt atttgtccat ctgttcctac tgttaaaacc aattctttta ctgcttgctg
     8281 atttttttcc aaacgccgca acatcccttt aaaggaattt tctgtcccgc tgtagccgtg
     8341 gaaaaaaagt gttggaatag tggttttttc tatcacttta ctggattctg ccgttttttc
     8401 tttatcagaa acttcagtag tatttatctt cgtcgtctgg caaccactta acgcaaacaa
     8461 actgataagg actaaagctg ctgcagttgc acgtttgcat aaatttttga ttggttctgt
     8521 catacacctc ttcctttcca tatagaaaaa agaaagactt gaaaggccaa tcatatgatc
     8581 gacatttcaa gtctctttat taatcgattt tatctacttg atcgaaatca agttctgtac
     8641 tggtttcacg tccaaacata tcgatgttga ctttcaattt ttctttttct ccgtcaactt
     8701 ctgttacttg tccttctaat ccagcaaatg cgccttcgat gattttcacg acttcgccaa
     8761 cttctacttc tagatcattt tgtcgcaggc tcataccgat agaacgcaag atatgattga
     8821 cttcttctgg aagtaatggt gctggtttgc ttccggctcc gtgtgaacca acgaatcctg
     8881 tcacaccagg tgtgttgcgg acaacatacc atgaagcgtc tgtcatgatc atttcaacaa
     8941 gtacgtatcc tgggaatgtt ttatgaacga tttctttttc tttcccattt ttgacttcag
     9001 tttctttttc ttcaggaaca acgacacgga aaataaagtc ggccattttc atactttgcg
     9061 cacgagattc gatatttgct tttactttgt tttcatatcc tgaatatgta tgcaagacat
     9121 accagttttt ttcaaaagat tccattgaag gctcctcatt tcattgtaaa ataaaaaaac
     9181 ctcagtacac ccgaagtttt tctttcgctt cattatagca ctaaaagtac gttgtggcta
     9241 gcgagttatt ttaaaatcca gccaaataag ctttggatgg ctgtatccat tacgtaaaac
     9301 atcaatccga aaataataga agtttcgatg actactaacg tatctttacg gagttgtttt
     9361 ctgcttggcc aagttacttg tttgatttca tcttttacgc tttttagaaa tttcatgaga
     9421 caacctccct tgctactgtt tatttcgttt ctttatgtaa tgtatattga ttacaatatt
     9481 tacaaaactt attgatttcc aaacgttcgc cgctttttcc ttcactgact gattttgtgt
     9541 agttgcgaga cccacaaacg gaacaagcga gtgctgcttt tttctttgcc atttacttct
     9601 gcccccttgc tgagaatcag acttatactt cataacagta tcacgtaact ttttacttgt
     9661 caatttgtga aataaactat ctattgtttt ggataaaatt atgatatgat taatttgaaa
     9721 ttaagctaaa atgagacaat tggaggtata gcgatgctat taaaaacact tgtatataag
     9781 aaaaaagatc tgacaaccgt tacagagact agtacattag aagaagcatt aaaaatttta
     9841 gaaacttccg gataccgttg tgtgccgatc ttagattctt caggtaatat tttccgtgga
     9901 aacatttaca aaatgcatat ctatcgtcat aaagcaaatg gcggtgatat gacgcttcct
     9961 gtcactcatc ttttgaaaaa cgctacaaag ttcatttacc tggatacttc tttcttcaaa
    10021 gtattcttta cgatcaaaga gcttccttat attgctgtcc ttgacagtga aaatcatttt
    10081 tatggtattt tgacacatag tacgttgctt aatatgctct ctcaatcttg gagcgtagaa
    10141 caaggaagct atgtactgac aattgcttcc acaggaaagc aaggagattt ggctgctatt
    10201 gcaaaaatca tttcaaaaca cagcagcatt gccagctgta ttactcttga tatcgaaaaa
    10261 gacgaataca ttcgacgtac attgatcacg ctaccggcta acactgaagc gaaagtatgt
    10321 gaaacgatcg tcaaccactt agagaaaaag aattataaag tggtagaaat tgaaaatttg
    10381 caaaaccaag aagcataatc aaaaagcgaa aaaagaaaac agctaatctg ctttcttttt
    10441 tcgcttttta taattagaca cgcttttcaa aatcaagcgc gcgatctaaa taatacatca
    10501 atggtgctga aacagccatg acgataaact cacttaatgc aagcgtgcca taggttgtcc
    10561 aaaatgcttg gccacctgtt ggaactaaca tataagcaat cagtcccatg ctgatggtaa
    10621 agaagatcgt gttcaatact aaacgcatct ttacattttt cactttgttt tgcaataaag
    10681 ctgtcaggcc taaagaaagc aatgtttggc cgccaccaaa aaccacatcc atgataccaa
    10741 aaccaaagaa ggcattatag acgatgaccc cgcctaaaac tccccaaagc aatttacggt
    10801 taaagacaac taaatgattc aaactttctg aaatacgaaa ctggatcggc cctgaggata
    10861 caggcgcaac taaaatagtt aacgctagat aaagagccat gatgatgcca tttacaacga
    10921 ttacggataa ccgttgtctg cttttcgttt tttgcatttt ctctcctctt ttctcctagt
    10981 ttttttaacg cgggatggtt gatgaaccgc ggttatgtga aacaaacaat aattataaca
    11041 gacttttcat ttatttcaat ccgtcttatc tgcttttaaa aaagcaaaag cttaaacaat
    11101 gtaaacgttt gctaaaaatc gtgggatatg gtataaagta agtgtaaggg gtcataagaa
    11161 ttcccagtaa agcagacgaa gtaggatctg caagaacaga attgttgtga aacttaaata
    11221 aataaaaaag gatgtgggaa tttgagcaac ctcttactag agaacggtct agcaagcagc
    11281 tgaagatcta agtggacatg ccgaaccttg gaacttctcc ttacgtaagt aatggatcat
    11341 gcgcttgcta ggcttttttg tgtttttcgg tcttcggacc tttttcattt tcctagactt
    11401 ctgctataat aaccaagaat taattttgta acagattttt ttgaaaaaac aagtttcttt
    11461 cattcaaaaa atagaaaaag cttgctaatt attggttaac cagcaaacag gagtattaaa
    11521 atatcttgtt ttattgaata gcaggaggaa atcatgaaat tccattatca tatattgatc
    11581 aatccatcgg caggcagcgg aaatggctat aaagtagcag aaagaatttt accggtatta
    11641 aaaaataagc atattgacta tacacttcat tatagtgaat acaaaggaca cgatgctgag
    11701 attgctgaaa ctttggcaaa agaaacattg attccttgga tagaagaaga acagaaaaca
    11761 atcgatactt tccccctttt gatcatcgta ggaggtgacg gcaccttaca tcaggtactt
    11821 gatactttct atcagatgga agtagaattc ccagttgctt atattccagc aggttctgga
    11881 aatgattttg ctcgaggggc aggccttcca aaaaatccca aaaaaggact tcagctgatt
    11941 ttagcagctc aatcaccaga aaaagtccac ataatggctt atgaagaaaa aataagtgaa
    12001 aaaaaaggaa tagctgttaa taatttcggt ataggattag atgcagctat cgtccatgcg
    12061 acaaatcatt cttccacaaa aaagcgattg aacaaataca acttaggatc attttcgtat
    12121 ttattctcta ttctccgtgc tttattcact caaaaaggat ttccaattct ggtagatgca
    12181 ggaggaaaac aattaagttt ttcaaacgca tttttatgta cagcaacgaa gcatcctttt
    12241 tttggcggag gaatcgcgat tgttccaact gctgatgcgt ctaaaccagt cattgatttt
    12301 gtcgtggtcg aacgaatcaa tttctttaaa atcttgtggc tcattctttt acttatacag
    12361 aaaaaacaat tgaaatcgaa atatttccat cactacacaa caaacagatt acgtatcgtt
    12421 tcaacaacac cgcaacatgg tcaagaagat ggagaagaaa tggaaaaacg accttttgat
    12481 atcacaataa ccacaactaa acagttgttt tggtgttaat aaaagaggtt ttgtcagaag
    12541 ctacatctgg aacaccgttt attcggaaaa aaggactgtg acacgacttt tgtcacagtc
    12601 ctttttttac tatgcagtta attctttgcc caattgggtt gccatcgata gtttctttct
    12661 ttacccaaaa taaattggat cattgaaaac accgcagcaa aagcaactaa gacacaacca
    12721 ttgaaccaaa aagattctgc aaatgtttga ttgtaactga gtgtttcact gatcaagtta
    12781 ttattttgaa caaaccaata tgttggtgtg aatgccgcta ttttgctaac catttctggc
    12841 aagtattctg ctggtacaaa caccccaccg ataaagcaac tgcctgtgat aaaaatatct
    12901 ttgatcccac cgctggtttc tgagtttttt accaatgaag tgatacaagc cgaaaagcta
    12961 atgatcggca ataaaaacaa gaaagtattt aaaatatgat accataccgt tatatcccac
    13021 gaggtgtgga tcactaacag aaccattaga agtaagaagc cactgaacaa cagcagcgag
    13081 taactaatca aactaaaggt tactttgcga ttcaagtttc gtttggtaat gggcgcactc
    13141 tggtttcgtt tactgatctc tttgcgatta aacgctagat tgatgacgct gaaaccgcta
    13201 aaaatggata gaaacaaccc ataagacaag aaattaaagg ttgtgcccgt caacagcttt
    13261 ttcatccgct gactatatcc cgcacttagc tcaacttctc cagtaaccga gagtgccatt
    13321 tgtgtctgtt ccatagctgc atcgattgtt ttatcttctt cttgaaacag aatcagtgtt
    13381 tgcaaaaagg tgttcacttg ctgtgtaaca actgttttag aaaaggcatc tgggcgactc
    13441 tgtatcttga tcgtcgccat ctttcctact tcgacttgtt cggtaaagtc tgcaggcaga
    13501 tgtaagataa tgtttacacg attaaaataa agagcatcat cgatttcttt ttgtgaggtt
    13561 ccatccaatg taacgactgt ttccttgtca ttcaaatatt tacgaaggcc atcgactaca
    13621 ggcgactctt cttcactaat caaggcgatt ttcgcatctt gcagagaggt attttcaaca
    13681 tcaatatttt gcgcaaaaaa gaatgtggca acaacagtga taattacacc tatcagtaaa
    13741 ttcgatttat ttgctttaag caatgtccaa atcgttttat aaagcgtcat aacgcacccc
    13801 tctttccatc caagcatttc ctaacgcaaa aaggaaaata aatccgatca accatagaag
    13861 attggtataa aaactagcca aatcagtgta gaaataaagt ttatacaaac tttcgctgat
    13921 caagttgacg agatttaatt gaccaacaat cggaatatga agatcaatcc aatacttcac
    13981 ttgttccgac cccatcatac cagcaaaaaa gctcatcgca atcgtcactg aaatcaagat
    14041 gctatccttt tgcaccagat tcatttttgg tatcaaatta ccaaccaatg tacctaacaa
    14101 aatcgcacac aacgacccca gagcggtaac aatcatcaac cattgccaac gttcaccaaa
    14161 gtctacttga tagataaaac gaaagacagc taacataata taaatctctg tcaaaacaat
    14221 cgtaaagctt gctgccaaat tagcgattga aactaacgat tgctttctag gaatcaagga
    14281 caagcgaata ccatttgacg attgatttgc ttgctgatcc tgtacattgc ggacccccca
    14341 catcgtcccg tagagaatcg ccattccaat cagagtgaag aaataaaaac ttttgaaact
    14401 catattacga cctgcatgaa cttcttgaaa agatacttct tgttcaaaaa cagtcatgat
    14461 gggcgcgaga tcaacgttct ccacttctgc cactttactc gaaattatac caatacgctg
    14521 taaaaattga tccatgacgt ttttcatcaa cgtctgactc attcctgctt gacccacatg
    14581 gagcgaaaca gatgaaccat caatgagata aaaaccagcc aaatcatctg catttagctt
    14641 ttcattcgct tctacttccg atagttcctg tacttcaaat aatggtttat cattgacttc
    14701 gacttgttct aagactgtgg taaaattttc cgaaagcgct gtttgatccg ctttgattac
    14761 aattccagta ttaatcgttt caagattgcc accttgatca atctcgccaa aggccgccat
    14821 aaacatcaat cctaaaagaa ttggaaaggc caatgtccaa aacaacatgg atttatttcg
    14881 cagcagtatt tttgtgcgat atttataaaa atgccaaaac attatttccc ctcctctagt
    14941 ctctcaaatc cttgccagta atctctaaaa agacatcatt caaggacgct tctttggttg
    15001 ctatttttcc gtaactgatc ttttcttctt gtaagattgt aagaatcggc aacaatactg
    15061 tatttagttc agtagttgtt acttggagct gacctttttt ataagcgatt ccatcagaag
    15121 ctattgttcg ctgaatcgct gacaacacat tttctgggag taatgggatg tcgatcgtca
    15181 aacgatccgc cgtccgtacc atatctttta gagattcttt ggttccttta gcaatcacct
    15241 gtccttgatc catgatcacg atctggtcgc aaagaatttc tacttcttcc atgtaatgtg
    15301 ttgtatagac aatggttgct ccttgtcgat tcagctcctt gatactttct aaaattttat
    15361 tccgactttg ggggtctaca gctactgtcg gctcatctaa aaaaattaac cgcggcttgt
    15421 gggcaatccc acatgcgatg ttcaaccgac gtttcaagcc accactaagt tctcctggac
    15481 gaaacgaaac atatttttct aatcccacta attgaatcac ctcttcaatc aaggctttcc
    15541 ttttctcttt ttcggaaaca tataaaccgc aaaaataatc gatattttct tgcacactta
    15601 gttcgtcaaa gaccgcaacg tcttgaggaa cgaccccaat cattgccttg acagcataat
    15661 tttcaggagt cattgttgtc ccgaacaatt taatggttcc tttgtcataa tttaacaaag
    15721 aaagcataca gttgatggca gtcgatttcc cactgccgtt tggacctaac agacctaaga
    15781 cttctccttc ttgtacttgc agatcaaaat gattcaatgc tacaaaatct ccatagcgtt
    15841 tgactaattt atcaatttcg ataatcatca tgaaaaactc cttccaaatt tcttgttgat
    15901 ctgactataa taaagtgcaa gaaaagaaac tagtgacgat tggctaaacc acacatgaca
    15961 tttgtcacaa tgaaggagtc tgctatgcat aaatctcggc tactcaacga aactagcttt
    16021 ctactggtta tctcattgct tttgctggta agtcaaggat tcgttgctat gcaagttatc
    16081 tatttattga tcgccgtttt attcactggt gtcagttatt tactgcctac aaagccaaat
    16141 tggattttcc aagttattct atttctttta tgtgcctttt tacctgatct tgtcctgttt
    16201 ctgccagcga cgatgcgagt gatcgcccct gaagaaagcc accttgatta cttgtttttg
    16261 ttaggttttt tgttgatttc gacggttggt aatcatcccc ccatcaaggt cgcctttgtc
    16321 gtaattttgc tgtttttttc cttttactta cgaaaaaaag atcaacgaac gaccgatctt
    16381 gagaaaaagt acattgtctc caaagatact cattgggaaa aacaacaaga gttatcggaa
    16441 caaaataaag cattggccct gtcacaagaa acacaattag ccttgcaaat tgtggaagaa
    16501 cggaaccgaa ttgcccgaga catccatgac aatgtcggtc atctgctgtc aagtgcgatt
    16561 ttacaaattg gtgcgcttga aatgatcaat caagaaccaa aactaaaaga accattagaa
    16621 aatttaagtg ccaccgttca cactgggatg gatcggattc gtcaaagtgt ccatgacctc
    16681 catgagacat cgatctcttt tcaacactcc ttagagtttc tgatcgctga ttttccttct
    16741 tctgttacga ttgaaggaaa cttgtttgaa ggattgaatg aaacgcagca aaactgcttc
    16801 ttaatggtca tcaaagaagc cttaaccaac agtatcaaac atagtcatgc caaaaccgtt
    16861 gttttacatt ttcgaacgtt gccggccttt tatcgcttgc aaatcacgaa tgatggcacc
    16921 agcccacaaa aaaattctgc tcccggtatt ggtttgaaaa cgatgcgtca acgtgttcaa
    16981 gaacaaaagg gacaagtcca tattttccaa gaaaaccagc aatttcagtt aacgatcatt
    17041 ttaccaaggg agggacacca atgataacag tagtcattat tgatgatgat ccatttgtga
    17101 cgaaatcgtt acaaaccatt ttagaaagca cgaaagagat ccgtgtgtta ggcattggtc
    17161 attgtgccaa ggatgcgcta gtcttatacg aaacccatcg tcccgatgtt ttgctgacgg
    17221 acatccgcat gcctaaacaa acgggcatcg atgctgccaa agaaattttg gctaaatttc
    17281 ccaaagccat ccttttgctt ttgacgacat tcaaagatga agaatacatc cgcgaagcat
    17341 tttccatcgg tgtcaaaggg tatttaatca aacagaactt gcaagcgatt attccttctg
    17401 tcaaatccgc ttataatgga caggccgtat ttggtaatga aatcatcgag actttcactc
    17461 aaatgatgaa gaataaaccg acgattgata gtcatgctta tgcttctttt agtgaacgtg
    17521 agcttgcaat catcaaagaa gtagctgccg ggaaaaacaa taaagaaatt gcagatgcct
    17581 tgtacttgag tgacggcact gttcgcaatt acatcagcca attactagaa aagctagatt
    17641 tacgggatcg cacgcaattg gctatctatt tttatcagca tttgcaataa gtcttatcta
    17701 acacaaaaaa acctgaatga tgctccttcg catcattcag gtttttttca catcttctat
    17761 tcaattgtcc cgcaaaccat ttcctcacga ttgaaagaat caaggattcg cagggctgcc
    17821 aggattcgtt ggattgttcg gcgtgttagg cggaaaaata ggcgccttgt ctaacgtttt
    17881 ttctagtttt gattcttcac gatcgtctcg ttctacatca ggactttcat tcaatttgaa
    17941 ttctttttcc atctcatcta actttttaaa gtcattgcca gattcttttg ctaatttcgc
    18001 taatttatct tcggtttctt tagccataaa gtaacgcccc ccttttgatt tcagcttagc
    18061 atatcacact gaaaaaacaa aagaaaacgc taaccaacga tcatttttag ctatttctca
    18121 tctttaaaat caagcatttt ttgccggtat aaaatcgtag ggagaactgt tttccatacc
    18181 aatcatcgga tctactaact ggttatcgat cacttctgct gtaagaacga aatctttaga
    18241 aacagcaggt tctacctctt ctttcttttc ttctccaaca aatgaagaaa acagatctgg
    18301 ttccactgtt tccattactt ggtttttcgt ttcattcgtc ccactgtcat cttctacagg
    18361 tagcttttct tttacaaccc gtggctcttt tgtatagttc ccatcttcaa atggatcatt
    18421 tccgaccaaa gaatcttcat aagctgctag tttggtccgg agagtgatat tcattttatc
    18481 attatccatg atccttgatt tcaacgtagt catccggctt gctgattcat tcgctacaca
    18541 agtgacatag gcttgctctt cccctaacaa aatcaacagt tccttactgc gagtcactgc
    18601 cgtgtagagc aaattacgct gaagcatccg ctgatattga tggaccatgg gcaaaataac
    18661 catcttgaat tcactgcctt gtgatttatg gattgagcaa caataagaaa gagtgatttt
    18721 attccattcg tttcttttat aagtgacctc atttgcatca aactggataa ctaattcatc
    18781 cactttatct tcagaatctt ttgccaagat gatacccgtg atttctccca tatctccatt
    18841 gaatacattc aattcgggag tattgactaa ttggaggact ttgtctccta tccgataaac
    18901 ggtgtcgttc caatgtactt ccttttttcc aggtttccct ggattgaata tctcctgcat
    18961 cattttattt aacgcatcga tccctgcagg tccccgatac attggtgcta atacttggat
    19021 atcttgtgca cggaatccct tatttttcgc tttttccaca attttagaga tcaagggttc
    19081 aatttgataa gcatcacttc ggaaatagga acgatctttt tgattctttg tgaaatcttt
    19141 tggcagacgt ccttctttga tctcatgggc aagaggaata atactagaac catcaccttg
    19201 ccgataaatc tgattgagtt ccattttggg aatttgttcg atttccaaaa gatcatggag
    19261 tacttgacca ggaccgacag agggaagctg atctttgtct cctactaaga taacttgcat
    19321 attcgtcgga atcgctttga ataatgtgtt agccagccat gtatcgacca tagacatctc
    19381 atcaacaatc aaaagtccgc cttctaattc tttcgtactg actgaaggtg ttttttcccg
    19441 tccattcagc cccagaagac gatggatcgt actggcaggc agacctgtcg tttcgttcat
    19501 tcgtttagct gctcggccag ttggtgctgc aagtagaata ggaaaaattt cttgggtata
    19561 ttttgaagga tccaattcca gtccgttcaa ctctgcaaaa agattgacga ttccgttgat
    19621 cacagtagtt ttccccgtac ctggtcctcc tgttaaaata aatagagggg aacggattgc
    19681 ttcagcgata gcttgttctt gcgaaggtcc atactgtatg ccgaatattt tttcgatacg
    19741 ccgaatattc ttctgcactt ctttttcagg ataatggatt tcttttttac gactgagaag
    19801 gcgctggatc gaattaccga tcccccattc agaaaagtac aaactgtttt cgtaaagatt
    19861 agtctcttct tgttgtatct ttccttcttc aacaagttgg ataattacag tagcaacctg
    19921 atcaggctgg atttcaattg ggcgacttgt ttctaaaata cggatcgttt gctgaagcaa
    19981 tgtctccgcc ggtacgtatg tatctcctgt ttgaactgca tgctgaaaaa tctgatgcaa
    20041 gatagctgct cgaattcttc tcggtgaatc tgctgcaata ccgatttgtt cggctagatt
    20101 atctgcacgt ttgaacccga ttccttctat gtcttcgact agctggtacg gattttcttc
    20161 gatgatatcc aaagactcgt tcttataggc ctggtagata ctgaaagcta actgactacc
    20221 gaacccgtaa cgattcagtc ccatgatcac ttgatccatc ccgtggttta gccgaatcgt
    20281 ctcagcaagc atttctcttt ttggcttcgt caaaccggag atttgtttta ataccgaagg
    20341 atcagcaatg attttttcga tagcatcttc tcccagcaat tctacaattt tttctgctgt
    20401 tttcttcccg attcctggga atttttcgct ggaaagatac gctacgatcc catttgcagt
    20461 tgttggcttt tcttgctcat atctttcgag tttaagctgc cttccgtatt taggatgatc
    20521 cactaattct ccataaaaac gataagtctc attttcttga atatcgccga aacttccggt
    20581 gactacaatc tctttttcac ggtaatcagc tgtggtttca ctgatgtgta ccaaaagcac
    20641 tttgtaaaaa ttactaggat tttgaaaaaa aatcgcggca actgtaccaa cgaaataagg
    20701 catttcttct ggctgcatcg gaagccgcct ccttcttcta tagtaaaggt tctggtgctg
    20761 tttcagttcc taaaaaatct gtttggttcc atactttcag ttcatatggc aataatctgc
    20821 aaggctcttc agtctctaaa atcgtcaaac tactattgaa caatccaccc atttcacgca
    20881 actcacttag ctcttttcct gtcatccatt gaatggctgc agttaaagaa gctccatgtc
    20941 ctacgaataa aagtgggcca tctttatgtc gggcaaccgc atctgctaca acagtttcga
    21001 tacgtgtgat tgccgattca atcgtttctc cgtgaaaagg agaaggatca tacagatcta
    21061 aatgattacg caaattcggc agattatccg gatatttctg gtacatttca tcgatcgact
    21121 gaccttctaa agtacccagc cctagttctc ttagcttatc tgtataaaaa atctctaccg
    21181 gacgagcaag ctctttgttg ataccttctg ctgtatcacg tgctcgcttt gctgtactgg
    21241 aataaattgc tttaaaagga acatatttta cctttttccc aaatgctttg atttcttcgt
    21301 aactttgtgg caaaagcgga gaatcgccat tgctcccttg aaaacgtctt tcctgattcc
    21361 attgtgtctt cccatgtcgg gtaaaatata gttccatacg cagcaaactc ctgtctttct
    21421 tttattttac tctataataa actgtctaat ccagcttgtc attttcatag tgatgcgtca
    21481 attctaaatt agggaatcct tgctggcgaa gtgcttcata cactatcatt gcagctgtat
    21541 tcgataaatt caatgagcgg acatgctcat cattcatagg gatgcgtaaa cacttttctt
    21601 cgttttcacg cataaatgtt tctggcaaac cggttgtttc ttttccaaat aaaaagaagt
    21661 gctcttctcc atcagcaaaa ttttcgtcac tatatacttt atgggcaaat tttgagatca
    21721 aatgtagttt acgattacct aaaaatgtta aaaaagcttc taaattactg tggtaagtaa
    21781 tatttacatc attccaataa tccaaccctg cccgttttag atgtttgtca tcagttgaaa
    21841 aacctagtgg ctcgattaaa tgaagcggag tattcgtggc tgcacacgta cgggcaatat
    21901 ttccagtgtt tgcagggatt aatggttcaa ataaaacgat atgattcatc aggatcattc
    21961 ctctcatatt tatgttcgtt tctattttct gacaatttga tttttgcata aaaaaaaacg
    22021 tatcaactcg gtgtcagtca ctgcgagtcg ttacgttagt gaagctcttc tcactaactt
    22081 ttatcagaac aggaacctga taaaaaccaa tgaaaaaaca acttgttgct aatataacac
    22141 cctcaacttt tttttgcaac ctctactcat taaaaactgc tagaaaaacg ttttctctta
    22201 gttcctttta ataattctat gtcaattgta atcttcttca aaggattctt atataagtct
    22261 gctttgattc gctcttctac ctgccattct aaaggtgaca tggccaaaag atctttttga
    22321 ttttcctctg gtatctcaaa ttcataggtt acacgctgac gttcagttaa tttcattgct
    22381 tcagcaaact tggctactac acgttcattg gaataactct gtttttcagg ttgattcgga
    22441 taaaaagcct gacgtagttc ctttaaataa ttttcttgtg gcaccacctt caacacttct
    22501 ccatcttctg agagtacacg ttggaattct tggtaatgag aaggcgaaaa gatatttaaa
    22561 accgcgttga atgtttgatc agcaaacggc aaattggtca aatcagctac acaccaaaat
    22621 gcttcggcag attgttctgt agctgcgtag actccttctt ttgaaatatc aaatccaaca
    22681 gcattttgaa tctttttcaa ttttgaaagt tcattcaaga agctaccttc tccgcagcca
    22741 acatctaata gcgagacttc ttcaggtaaa tgctgcgcaa tagtctcaat aacgggctga
    22801 tacatgccag attggatcat tctacgcctt ggaacgaaca ttccttgatc gtattctgtt
    22861 ttaaccgcat gggataaaaa ataaagtgtt ccttttttgg aaaggtcaaa acggtgatta
    22921 tttttgcata tcaagccatt atcagacttt tttacttctt cttggcaaac cggacagcgg
    22981 aacagctgat gattttctgc caaaaactgg cgtcctcggt caattttttt taacatagtt
    23041 ccccctcatt tctgttttat cttaccgtta gatgctcact ttggcaactg ctaaaaatat
    23101 gatacaatgc ctagaagaaa gtgaaagata aaggagaaga ttagatgatc aaagaatttt
    23161 gcgctgaaaa ctacacatct attcctctag caattgctag aggagccgat cggatcgaat
    23221 tgtgtgacaa cttagcggtc ggtggcacaa ccccgagtac tggtgtgatc gaagaaacgt
    23281 taagttatgc tggggaaaaa gatatccccg taatgacgat tattcgtcct agagggggcg
    23341 acttcgtcta taacgatatc gaattgaaaa tcatgcatac tgatttgatt gaagcaaaga
    23401 aattagggac ggatggcgta gtatttggtt gtcttactcc ttcaggctgg ctagacgaag
    23461 aggccttaga actattgatc gaaacagcag aagggttgca gatcaccttc catatggctt
    23521 ttgacagtat tcctgaagat cggcaattcg aagcaatcga ttggctggta gaacatggtg
    23581 ttgaccgtat cttgactcat ggcggtcctg ctgacaaaac tattgtagaa aatttcccac
    23641 ggcttaaaga attgattgcc tatacagatg gccgcttaat cattttgcct ggtggtggtg
    23701 tcactgcaga gaatgcagca catgtagcta aagaattgaa cgtaagcgaa gtacacggaa
    23761 ccaaaatcgt ggatcttcag ttataatctt ataaacaaaa aaacatttgc aacaggcata
    23821 atgcttattg caaatgtttt tgttttttta cataaaatag cccataaata aagaagcaat
    23881 attgaaataa atcagtacac tcgtcaaatc gctcaaagtc gtgataaagg gaccgcttgc
    23941 tactgctgga tcaaatccta gacgatccat tagcatcggg attaaactac ctgcaagatt
    24001 cgccactgtg attgcaaata gcatagccat ccctatgaca aaccccaaga taaaattatg
    24061 tttccaaaaa cctacgatta gaaaaatcgt gatccccgta acagctcctg tgacaacgcc
    24121 ggtcaagact tcagccaaaa caagtcgaac aaatccatga tctttctcat cagaaatcgc
    24181 aagtcgacga acagccacag ctagcgactg ggttccagca ttaccggcag ttcctgtaat
    24241 cagcgaaatg aagactgcta aaatactcgc ttcacttacc aagtcttcat agtgactgat
    24301 taaagaagcg gtcgacatac ctaaaaacaa cagagtgatc agccaaggca aacgtcgaga
    24361 cgctgccttt actggatttt cattaatttc ttccacattg accccggcaa gtcctgaata
    24421 atcgctagca gcttcatcat caataacgtc gatgatatca tcgactgtga cgattcctaa
    24481 caaatgatca tcataatcgg taacaggaag agctaagaag tcataatctc taaatgtttg
    24541 tgccacatct tcttggtcgt cgcctacatg aactgaaagc actcgttcgc tcatcagatc
    24601 agaaatcatt gcgtcgtcat cattcacgat taggtcgcgc agagaaataa cccctactag
    24661 ctgaccttct tgattaacga catagatgta atagattgtt tcagctacat ctgcttcgtt
    24721 cttcagcacg tacatcgcag agcgaacagt ttgattggct acgatcgaaa caaattcagt
    24781 tgtcatgatc gcgccagccg tttcatcctc gtaatggaga agttccttga tttcactggc
    24841 atcgtctgcc gacatcaaac ttaaatattt agcaatttgc tttttatcca gcgtattaag
    24901 caagtcaaca gcattatctg tatacatctc agaaagcata tcagctgcat agctaggacg
    24961 catttcatct aggtattctt tcatatgttc gttgtcttct tcaatcacat cgaacatatc
    25021 cgctaattct ttaggcgaca gataggaata gatcagctgc ctgttctctt ctgtaagaga
    25081 ttggtagaat tgcccctgct catagatatg catctctaaa aaactgtttc tgaatgcctg
    25141 aatattattt tcccttaaat cttgagtcaa ctgggcaaat ctttcctcta attcttgatt
    25201 ttcatccaaa ccaatctcac ctcgccttat tcttcaaacc gtctcacaac tgcctccatg
    25261 tctgcggcta aaggctgctt gaactccagc aactcttctg taaatggatg gtaaaaacgc
    25321 agatcataac aatgaagggc ttgtcttttt attcccagat ccattcgccc gccatacata
    25381 tcatcaccga gcagggcaca gccaattgcg gaaaaatgaa ctcttatttg atgtgtcctt
    25441 cctgtatgca gctggatatc aactaacgcg atttctgtta gtcgcttctc tatccagtac
    25501 tcagtctgtg ccgaacgtcc ttcatcagag accattcgct tcaatagcga agaataatct
    25561 cgggcgatcg gcaaatcgat atgtccatgt gccgataaat ctgaaagcct tccactgact
    25621 aaggcctgat attttttcac aaacttcttt tcacgaagtt gcatatctaa ttttgcgtgc
    25681 gcaaatccat gctttgcaaa aagcatcaag ccagatgtat cccgatctag tcgcgtcacc
    25741 acatggatta cttggttttc gtaaccttgt tttttataat aagcctttac acgattagcc
    25801 attgtcccat ttggatggta ttgggcagga atagacgaaa cgcctgctgg tttatttact
    25861 accagaacgt gttcatcttc atacacgata tcgatcggtg tttcatccaa aagtacggtt
    25921 tcatgttctc cttcagcagg aatcacaatg gttaccttat catccttagc tagagaaaac
    25981 aagacatttt ctacttgatc attaactttg atttgaccgc cttgaaattt tattttcgct
    26041 aacagaccct ttgagatccc tttttctttt aaaaaatatt ttacttgctg agaatgatct
    26101 ttctcataat cccatgtaaa ctgcatatca atcctcacta ataaaggcat ctttcacacg
    26161 atgccaaaaa tgcatatgtc gataagaagc aaaatggatc cgttcttctg ctattcgata
    26221 ataaattgag cggatatttg cttttgcgac attcaattga tcaaccgtta caagataatc
    26281 ttctgtattt tccaatttta tttctagcca ttcatcatgg gcaatcacga tcggagaacc
    26341 caaagtacga aacactcgat tattcagtga agcaatctct gccaactgaa aggcattgat
    26401 actaggatgg atcacagcac cgccgacact tttattatat gctgtcgaac cagtaggcgt
    26461 agaaatcgtc aatccatctc ctctaaaact ttcaaacagc tcgtttttga tgtatacatc
    26521 ggctaccatc gtacgattgg cccgtttaat cgttgattca ttcagtgcta aaaaatgctg
    26581 gtccggtgtt tcatccaaat aactgatccg aacatccagc aagggataac taacactttg
    26641 ttcccggttc gttcgtaagc tgtctactag ttcttccagc tcgtaatccc gccaatccgt
    26701 gtaaaaccct aaatgacctg tatgcacgcc caagaaacgc acatcattca gacaatgact
    26761 gtagtgatgg aacgccgaca ataatgtgcc gtctccgccg actgagatga ccaattctgg
    26821 ctcatgatca tctcgttgaa tccctgcttg ttctaaaagt gcgttcaaac gttctgtcac
    26881 ttctatagtc tttgcttctt tattatgaac gatcgctact ttcatcccct gcctcctctc
    26941 tttcgctttc tttcttcaaa agcgttcgat agtagacatc atctgatttt cctcttccat
    27001 gggagaataa ccgctgtgct tcttggattt cttcacggat actggacatc tcttcatcca
    27061 actgataggc agcttctgcc gcacgctgaa gtcgttcact catttcttct ggaaaaacgc
    27121 cttgatattt ataattcagc gaatgctcaa tcgttgccca gaaattcatc gcaagagtcc
    27181 gaatctggat ttccgccaaa attttcttct caccgctgat cagctgtaca ggatactcga
    27241 tcaccagatg ataagaacgg tagccacttg cttttttatt tgtgatgtaa tctcgttctt
    27301 ggagaacttt catgtctttt cttttacgga tgatctcaac aacttgatgg atatcttcta
    27361 caaactgaca catgatcctt aacccggcaa tatcagacat ttcttcttct aaacgtccca
    27421 ttggaatatg ccgtaattgt gattttgtta caatactatc gactggcttg actctcccag
    27481 tgacaaattc aatcgggaca tgtcgatttt gttcacggaa ctgtttgcgg atcccccgca
    27541 gtttcacttt caattctgat acagtttgtt catatggtgc taaaaatgct tcccaatctc
    27601 gttccatagc tgatacctct acttcattaa cattcattgt ttattctacc atatatgcaa
    27661 agttttttcg tactcttgat ttatgcattg accaaaaaaa gaaaattagg caaaatagac
    27721 aaaaaggagt cgatcaaatg acacaaatgc ttgaaatcga atataaatct atgctgacaa
    27781 agcaagagta cgagatattc atcgctcact atcaattaac agataaagat ttccgtgtac
    27841 agactaatat ttatttcgat accgttgatg aacagctgaa gaaacaaaat tgtggcctac
    27901 ggatccgttt cgttggtcat caagctgaat atacattaaa aacacctgct gaaaaagggc
    27961 gtctcgaaac aacggatacc tttataacag aagaagcaga agcatttatc ttcgaaaaac
    28021 gtctgcctcg ttcaggcgca gtttttcaaa aactttctga tttgaatatc gatcctgctt
    28081 cattaatcca aaccggaaag cttacaacaa aacgagctga attccctatt gaagaaggac
    28141 ttttagctat tgatgaaagc tggggagaca atctacacga ttttgaactg gaactagagg
    28201 taggggatgc atctgtggga aaaattgctt ttataaattt tttaaaacgt ttttctttac
    28261 cttatcgtcc tgctaagaac aagatccaac gtatgcttga agctaaaaat tagaaacctt
    28321 tcattttcac aaggcacttg agttcatttt tttacttttt tctgttaagt tataggatgt
    28381 gaaagaatct agtcactagt tgtatagaat ctattttggg ggagattcat atgatcgaaa
    28441 tctatttatt tgttaatcct ttaggggaat actgtttcga aatggaacaa cagttattac
    28501 aatttatcga agaagagtac gggacaccat caaaagaaaa gatgcaattt cgctttcttc
    28561 ctttggtcaa tttgcagaca attggggatg tcatgcaaca aaaaggaatt tctcagaaca
    28621 atcttgagga acgtaatcac ttattctcaa tgacttattc tgcagcattg gactgcaaag
    28681 cggcgcagtt tcaaggaaag aaaaaaggcc gccgtttttt gatcaaattg caagaagcag
    28741 tcggctgtaa tgacatacca tattctcaag aacttgctga atccatattt gaagaaatcg
    28801 gcggcgattt gaatatgttt aaagaagatc gtcagtctga cttcgttaaa gaagtattct
    28861 tttctgatca aaaaattgct cgggaaatgg gcatcacaaa acacccttct gcagtcgttt
    28921 acaattatgc ctgcgaccgt gattttggtg ttttagtaga agtaaaaggt acaagtagcc
    28981 tttggagaat cacagagctg tgtaaaacaa cagataactc atatcaagtt tttaatggtg
    29041 aacatttaca tacaaatgat tctcagttcc attcaagaac tagccattta cacttgcttt
    29101 ctaa
//
LOCUS       DACDPS010000029        27787 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.029, whole genome shotgun sequence.
ACCESSION   DACDPS010000029 DACDPS010000000
VERSION     DACDPS010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.029"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            226..1485
                     /gene="lysA"
                     /locus_tag="IWJ07_001366"
     CDS             226..1485
                     /gene="lysA"
                     /locus_tag="IWJ07_001366"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAP9332955.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1472..2272
                     /gene="dapF"
                     /locus_tag="IWJ07_001367"
     CDS             1472..2272
                     /gene="dapF"
                     /locus_tag="IWJ07_001367"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAP9332956.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2275..3156
                     /locus_tag="IWJ07_001368"
     CDS             2275..3156
                     /locus_tag="IWJ07_001368"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAP9332957.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3153..3869
                     /locus_tag="IWJ07_001369"
     CDS             3153..3869
                     /locus_tag="IWJ07_001369"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAP9332958.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3875..4570
                     /gene="dapD"
                     /locus_tag="IWJ07_001370"
     CDS             3875..4570
                     /gene="dapD"
                     /locus_tag="IWJ07_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAP9332959.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4586..5587
                     /locus_tag="IWJ07_001371"
     CDS             4586..5587
                     /locus_tag="IWJ07_001371"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAP9332960.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5584..6945
                     /locus_tag="IWJ07_001372"
     CDS             5584..6945
                     /locus_tag="IWJ07_001372"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAP9332961.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6905..8056
                     /locus_tag="IWJ07_001373"
     CDS             6905..8056
                     /locus_tag="IWJ07_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAP9332962.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            8031..9200
                     /locus_tag="IWJ07_001374"
     CDS             8031..9200
                     /locus_tag="IWJ07_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9332963.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9375..10754
                     /locus_tag="IWJ07_001375"
     CDS             9375..10754
                     /locus_tag="IWJ07_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9332964.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            11035..11832
                     /locus_tag="IWJ07_001376"
                     /pseudo
     CDS             11035..11832
                     /locus_tag="IWJ07_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            12021..12689
                     /locus_tag="IWJ07_001377"
     CDS             12021..12689
                     /locus_tag="IWJ07_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAP9332965.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(12889..13275)
                     /locus_tag="IWJ07_001378"
     CDS             complement(12889..13275)
                     /locus_tag="IWJ07_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332966.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(13247..14629)
                     /locus_tag="IWJ07_001379"
     CDS             complement(13247..14629)
                     /locus_tag="IWJ07_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP9332967.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(14785..15972)
                     /locus_tag="IWJ07_001380"
     CDS             complement(14785..15972)
                     /locus_tag="IWJ07_001380"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAP9332968.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(15979..17010)
                     /locus_tag="IWJ07_001381"
     CDS             complement(15979..17010)
                     /locus_tag="IWJ07_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP9332969.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(17026..18480)
                     /locus_tag="IWJ07_001382"
     CDS             complement(17026..18480)
                     /locus_tag="IWJ07_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9332970.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            18655..19560
                     /locus_tag="IWJ07_001383"
     CDS             18655..19560
                     /locus_tag="IWJ07_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9332971.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            19651..20328
                     /locus_tag="IWJ07_001384"
     CDS             19651..20328
                     /locus_tag="IWJ07_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP9332972.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            20609..21505
                     /locus_tag="IWJ07_001385"
     CDS             20609..21505
                     /locus_tag="IWJ07_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9332973.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(21560..22930)
                     /locus_tag="IWJ07_001386"
     CDS             complement(21560..22930)
                     /locus_tag="IWJ07_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9332974.1"
                     /translation="MEEKKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            23168..23914
                     /locus_tag="IWJ07_001387"
     CDS             23168..23914
                     /locus_tag="IWJ07_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP9332975.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            23911..24750
                     /locus_tag="IWJ07_001388"
     CDS             23911..24750
                     /locus_tag="IWJ07_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP9332976.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            24751..25701
                     /locus_tag="IWJ07_001389"
     CDS             24751..25701
                     /locus_tag="IWJ07_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP9332977.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            25842..26594
                     /locus_tag="IWJ07_001390"
     CDS             25842..26594
                     /locus_tag="IWJ07_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9332978.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            26613..27764
                     /gene="queG"
                     /locus_tag="IWJ07_001391"
     CDS             26613..27764
                     /gene="queG"
                     /locus_tag="IWJ07_001391"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAP9332979.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         9124 a         5053 c         5833 g         7777 t
ORIGIN      
        1 ttcatcacta aatagtgttg aatttatgtt tttatgtata aatagcttct aatagttcgg
       61 gtattttgtt tttataacca tttttattct tatttttaaa aaaatatttc gaaatatact
      121 agacaattca gaaaattttg tttaatatta ttccaataaa attcaaatct ttatcaaatg
      181 aaattccaat aaaaacggaa aaaaagtgaa aagaggaatg aaaaaatgac agaattgata
      241 attggcggag tcacagcaag cacattggca gaaaagtata agacacctct gtatgtgtat
      301 gatgaagaaa agatgagaca aacgatgctg tcattcaagc gaggctttgt atcgaaacgt
      361 tttgatacaa aagtgcttta cgcctctaaa gcattccaaa cgattgagat gctgcattta
      421 gttcgtgaat atggattcgg gctagatgtt gtcagtggtg gggaaatcta taccgctttg
      481 caagctgatt ttcctagaga tactatctac ttccatggta acaacaagac gccagaggaa
      541 ctgatctatg cacttgaaaa tgatttgttg catattgttg ccgataattt gatggaagtt
      601 gaattattag cagaattaag ccaacgctat ttaaaaaaaa tctgtatcat gcttcggttg
      661 aatgtcggag tagaagcaca tactcatgaa tatatcgtca ctgcgcatat tgattcaaaa
      721 ttcggcatga gctatgaaag taaagagtgc caacaagccc ttcagttgat aaaagaaagc
      781 agctatctag agttagaagg atttcatgct catatcgggt cacaaatttt tgatgttact
      841 gcttggttag ctgaaatcga caaattgacg aattacttaa ccgattttga ccaacctctt
      901 tccttgaatc ttggcggagg gtttgggatt cgttatacac aaaatgatca gccgatccca
      961 gtcgaagagg cactaaaaaa actgattgaa tatacggaag aagctttgga aaaacgaaaa
     1021 ctgaccattc gtcaattact gatcgaaccg ggtagaagta ttgttggaga agctggtaca
     1081 acactataca cgattggttt catcaaaaag acaccacata aaaaatatta ctttgtagat
     1141 ggcgggatga cagataatat ccgtccagct ctgtatcagg cagaatacga ttgtgattta
     1201 gcagcaaaac tgtcggaaga aaaaacagag aaagtgacga ttgcaggaaa aatgtgtgaa
     1261 tcaggcgatg tggtaattaa agaagcttat ttgccgaaag cagaatcggg tgatctttta
     1321 gctgtttatt ccacgggagc ttatggatac tcgatgagca gcaactacaa tcgagcgaca
     1381 cgaccagcag ttgtttttgt caataatggc caatcaagac tgattgttcg cagacaagca
     1441 ttttctgatc tactaagggg ggaagtttcc tatgatcctt cataaatata atggctgcgg
     1501 aaatgatttt atcttattag attacgcaga agacacagat tatagtcaat tagccgcttc
     1561 tttatgcaca aaagaaaaat atgatacaga cggattgatc gccgtaaaaa tcgatccgtt
     1621 ggaaatgatc tattacaaca aagatggcag ccgcgcgccg atgtgcggaa atggcattcg
     1681 ctgttttgct cgatacgtaa atgatcagaa catgatgaat gaacaggaat tcaacgtaga
     1741 gacattagca ggcacaagaa aaattacggt gttggatccc tcaccttttt attgcagcgt
     1801 agatatggga gtgccagatg attctccgga aaaaatcaaa gctaatcaaa cttatccaat
     1861 aaaaaaccag gaaatagaga tcaatggtga aaaagtaacg atcactagtt tatttttagg
     1921 aacgattcat accgttgttt ttgttgaaaa tgcgcgtgag gaagtctgga gaaaaagagg
     1981 tgaactgatc tgtcatcatc ctttgttcaa agaaaaaacc aatgtcaact ttgtagaagt
     2041 agtcaatgaa caagaattga tcgtccgtac ctatgaaaga ggagtaggtt ggacacttgc
     2101 ttgcggtaca ggttgctgtg cctcttatgt ggttgctaag gaaactggga aaatttcagc
     2161 agatcaggta gttgttcact tggaacaagg agacttgatg atctctggtg aggatgcgat
     2221 acaaatggct ggcccagcag ttcatgaatg gacaagaata ttggaggaat gaagatgata
     2281 aaaggttcga tcgtggcgct gatcacgccg atgaatgaag agggatcagt agattatgct
     2341 ggtttggaaa aattgatcca attccattta gatgaacaaa cagatggatt attagtttta
     2401 ggtacaacag gagaatcttc tacgctcaca caatctgagg aggagcagat ccttcaactg
     2461 acagtaaaga aagtggctgg ccgtgtgccg gtgattgcag gagctggaac gaataatacg
     2521 aaagaaacga ttgaaaaagc gaagcatttt gcttctttgg gggcagacgc acttttggtc
     2581 atcacccctt actacaacaa aacaagtgat gcaggactag ctgctcattt tacagcaatt
     2641 gcagaagcct caccgattcc gcttattttg tataatgttc cttctagaac aggcatgtcg
     2701 attccaatcc atgtattagt aaatttagca gaacatccta atatcattgg tttgaaagaa
     2761 gcctcaggag atatggctta tgtgatggat gccgctcggt tgattggaga agaattcttc
     2821 ctttacagtg gaaatgatga tttgatccta ccagtaatga gtgtaggagg aagcggtgta
     2881 ataagtgtat gggcgaatat ccaacctaaa atagtccatg aattagtaaa agatacgcag
     2941 gacggaagat ggcagcaagc aaaagaaaag caattaaatg cacttgaact gatccatgcc
     3001 ttgttcagtg agaccaatcc tattcctgtc aaagcagcca tgtcattatt agacttacct
     3061 tccggacctc tacgcctgcc tttagtttct ctttcaaaag agaaaaagaa acaattagct
     3121 cagcttttgt taaaagaaag gacggttgaa aaatgaaaat cggactgatt ggcagtggac
     3181 agatgggaaa tagagtcgaa gaagttgcac aagaaagagg agatacggtc cttcttttac
     3241 ggacggcacc taaagaagca actacgcttg cttttacgga actgccagaa ctgcttattg
     3301 atttttctca tccagataat ttagagaaaa tcctttcgta tagcttatca tatcaacttc
     3361 cgttagtcat tggcacgaca ggttatacac aatcacagtt ccaagaaata aaagaacatg
     3421 ccaaacatgt acctgtgatg tatagtgcta atttttcgtt cggcatcatg gtgatgaatc
     3481 aactaatcaa gcaagcagct gcaatgttgc aaggctggca gatcgaactg attgaaaaac
     3541 atcatagtcg taaaaaagat gcaccatctg gtactgcaaa tatgctgctt caaacaatcc
     3601 aagaagtgca aaagctgcag cccgtataca actggcaagg gaaaaaaaga gaagaaaacc
     3661 aaattggtgt acacagtatt cgtgcaggtt ctttacctgg tgaacatgat gtattgtttg
     3721 ctgctactga tgaaatattt acgatcaaac atgaatcttt ttctaatcga atctttgctg
     3781 caggcgcagt agaagcagcg gactggttga aagatcagtc accaggttat tacaaattag
     3841 aagatttatt aatggacaaa ggggtgtaaa aaacgtggaa gctcaagaaa tcatccgcta
     3901 cattcaaacg gcatctaaaa agacgcctgt gaaagtctat ctgaatgtaa ccgagccaat
     3961 cgaatttaaa gaaagtaaag tttttggtga aggaagcagc ttcgttgttt ttggtgacta
     4021 tgagacgatt gctccggttt tagaagctga atcagaaaaa atcattgaaa tcgaaatcga
     4081 aactgctgca agatacagcg cagttccttt gctagatatt aaaaaaatta atgctcgtat
     4141 cgaaccaggt gctattatca gagatcaagt gtcaatcggc aacaatgccg tgatcatgat
     4201 gggagcgatt atcaatattg gtgcagtaat cggtgaaaac acgatgattg atatgggtgc
     4261 tgtcttaggt ggacgagcga cagtgggcaa aaactgccat attggtgcag gagctgtctt
     4321 agcaggtgtg atcgaaccag catcggccaa accggttatc gtagaagacg gcgtactggt
     4381 aggtgcgaat gcagtgattg ttgaaggtgt ccatatcgga aaagatgcag tcgtggcagc
     4441 aggagcagtg gttttagaag atgtggcagc agaaactgtt gtcggtggaa tacctgcacg
     4501 tgtattaaaa ataagtgatg atcagacaaa agaaaataca gcattgattg ctgcgttacg
     4561 cgaattatag aggagatgga gaaaaatgcg cgaaatagat acctgtattg tgggggcaac
     4621 cggattagtc ggccaaacga tgctcaaagt gttagaagaa tatgattttc cagtaggacg
     4681 attgaaatta ttagcttcaa aacgttcagc cggtaaagtt tgtgtattca agaaaaagaa
     4741 acacactgta caggaattga cagaaacttc atttgaagga tatgatttag cgctattttc
     4801 tgcaggtgct tctgtatcca aagagtttgc gccaatcgca cgaagtcagg gactatttgt
     4861 catagataat tcctcagcat ggcgggaaga ttcggatatt ccattaatcg ttccagaggt
     4921 caatctggaa gactatacat tgaatcggct gattgcaaat ccaaattgtt caaccattca
     4981 agcggtactt ccgcttaaag ctttacaaga agcctttggt atccagcgag tcaattattc
     5041 gacctatcaa gcagtttctg gttccgggca aaaaggcatt gaagatcttc atcgtacgca
     5101 aaatgacgag gaaccagttt tgtatcctta taatattagc caaacggtga ttccggaaat
     5161 cgatcagcct ttggaaaatg ggtatacaaa agaagaacag aaaatgataa atgagacccg
     5221 taagattctg catcagcctg ctttgccagt atcagcaacc tgcgtacgtg tgccaatcga
     5281 aaatggacat ggagtctctg tggcagttca actgcaaaaa gagtttacga cggaagaaat
     5341 tagagagtgt ctcagccagt ttccagggat tcaattagaa gatgatctag cagcacataa
     5401 atatccgacg agcattcttg ctagaggaac agatatggtt tatgttggga ggatcagaaa
     5461 agacacttct ttagagaacg gattgttgtt ctatacgact gccgataaca tacgcaaagg
     5521 agccgctgcg aatgccgtac agattgctgc agctttaatg cagaaaatag cggaggtggc
     5581 aagatgagta atacgctgaa agtagcgaaa ttcggcggaa catcgatgtc tcatgaagag
     5641 caattcaaaa aagtccgtac aattgttctt tcagatccca atcgccaagt gatcgttgtt
     5701 tctgctttag gcaaacgtac gtcagatgat gacaaagtaa cagacttact ttatttgatt
     5761 tatgcttacc ttcagcatca tgttgattgg acaccccttt ggaaaaggat ttgtgatcgt
     5821 ttcattcaag taagagatgt tcttgccctg cagttaccta ttgaagatga actagcacgc
     5881 atacagaaac aactggaaga cgaaaagata actgaagatt atttagtaag tagaggtgaa
     5941 tatctgactg ctcaactgat ggctgagtat cttggttacc agtttattga cgcaaaagac
     6001 ctgattttct ttgactatga tgggaaaatc gatcaggaaa aaacagcaca ggaaatcaca
     6061 aaagcattta aaagatatcc gaaaatggtt gttccagggt tttacggggc gaaccctgca
     6121 ggaaaagaaa aattattggg ccgtggggga agcgacatta ccggcgcgat actggcgaag
     6181 atgttgcaag cggaaagtta tgagaattgg acggatgttt caggaatcat gatggctgat
     6241 ccgcgaatca tcgatcatcc taaaatcatc gaggaaatct cttttagaga actgagagag
     6301 atggcttata tgggggctaa cgttcttcat gaagcagctg ttttcccagt tcaagaggct
     6361 gatatcccca tccaaataaa aaataccaat gatcctttcg ctcaagggac aaaaatatcg
     6421 aatcacgaaa tagaaaagga aaatggactg acaggaatag ctggacgcaa ggattttctt
     6481 tccatcactt tattcaaacg tcatatgtcc gatgaaatcg ggtttatctg gaaagcaatg
     6541 agcatttttg ctaaacatga gatatcaatc gaacatattc cttcagggat cgacaatatc
     6601 ggagtgatcg tctcagcaga agcgattgcc gataagttat ttctcatcac gaaagaatta
     6661 aaagaaacac ttggcgtgga agaaatcgtc gtgatcgaag acttagcttt gatctctgtc
     6721 gttgggggtc cgcataaaga attgattgga ttatctggga aagtgttatc gatattgaac
     6781 aaattagaaa ttcgcacatc gatcttatca caaggagcac aggagttgaa tctgatcatc
     6841 ggtgtaccga ataatcaata tgaaacagta gtgaagggta tctatgaagg gatggtgaat
     6901 acggatgctg aaagaatatc gaatggcact ccatcaaatt cctgaaatcg ggtttcatga
     6961 attcaagaca aaaaaatatt tatatgaaca agtaaaaaac agcggggccg ttatccatga
     7021 aatcggcgaa acaggactac tcctttattt tgataatcag caagaagaaa cgattgcttt
     7081 tcgaacagat atcgatgcac tgccagtcaa agaagaaacg aatctctctt ttgcttctaa
     7141 gcatgaaggt tatatgcatg catgtggtca tgacggacat atggcgatgc ttttagggtt
     7201 gactgacttt ataactaaac acattgagga tcttatttat aatgtagtac tgatctatca
     7261 gccatcagaa gaaatttccg gtggcgcaca aagcgttatt cagtcaggat tgttggataa
     7321 atatgaagta caagcaatat ttggctttca tctatggcct ggactagcta agggagaagt
     7381 tttctctcga ccaggacctt tgatggcaca aagcagtgaa acagacatta tcattaaagg
     7441 ccagtctgca catattgctt ctagtgaaaa aggaatcgat agtctggagg cagctgtacg
     7501 atttttgaag aatgtttatg attttgaccg gcaattaccg gcagagtgga atcatctaat
     7561 gaaatttggc caaatccaag gtggaacgat ccgaaatgtt ttagctggag aagttattgt
     7621 atccggaagt atccgttctt acagttcaga tatgcaaaat tatttgaaga ggggattaga
     7681 ccatttagca gaacagtttc actctacagc tactatttct tttcgctata atgacggcta
     7741 tccagctgta atcaaccata gaggcttata tgcagcactg ttagattcaa acaaactaca
     7801 tgaattagat gctccagttc ttcaagccga agattttggc atatatacgc aacaatatcc
     7861 atgtgtattt ttctttttag gtgtcggtga tacccctgct ttacatgatc cgacgttcga
     7921 ttttgatatg gatgttttag aaaatggtgt agaatggtac aaaacgattc tttttacaga
     7981 aaatcttctt gtaaaaatcg atagcagttc aaaagaggag cgagtaaata atgatgaatt
     8041 tatccaatcg atttagtcct aggctttcta gaatagaagt atcaaaaata cgacaattcg
     8101 atcagcaaat atcgtcgata ccagatgtga tcaaactcac cctgggagaa ccggattttc
     8161 caactcctga acatgtcaag caagcaggaa tagcagcgat tgaagaagac ttttctcact
     8221 ataccggaat gagaggatta gaggaactgc gggaagcggc ttgtatattt cagcaacagc
     8281 gttatggatt aacttatgat ccgcaaacgg aagttttgac gacagttggt gcaacggaag
     8341 cgattgcctc tgcgttgtta tctgttttgg aagaaggaga caaggttctt attccagcgc
     8401 ctgcttattc aggatatcag ccattagtgg aactagctgg tgctgaattg atcccgatag
     8461 atacttctga cacagggttt gtctgtcagc cggaacaatt tgaacgagca tttgaacaat
     8521 atggatcagc tgtcaaagca gtcattttaa actatcctaa caatcctact gggacgacat
     8581 tgtctgcaaa acaattgaaa gcaatcgctg aggttttaaa aaaatatccg gtatttgtta
     8641 taagtgatga agtatatgct gaattgactt atagtggaac gcatatgtcc atagctactt
     8701 atcttccaga acaaacaatc gtgataagcg ggttatccaa gtcacatgcg atgactggtt
     8761 ggcgagtggg ctttattttt gctcaaaaag cccttattga cgaactaatt aaagtccatc
     8821 aatatctggt gactgccgct acaacgatgt cgcaaaaagc tgcggctgaa gcgctaataa
     8881 atggggtgaa agatagcgag aatatgaaag agcaatatca aacacgaaga gactatctgg
     8941 tccagcaatt ggcgccttta ggatttgaag tgacccagcc aaacggtgca ttttacttgt
     9001 tttgcaaact cccagctgcc attcaaactg actcttggca gttttgtgta gaattagcgg
     9061 aacaggcaaa agttgcgtgt atcccaggaa ttgcatttgg cccagaagga gaaggttata
     9121 tccggatttc ttatgctagc agcatggaaa aattacatga ggcctgcaaa cggatcaaag
     9181 actttttaac agaacagtga ttcatagatt ttatcaaaaa aacaatcgtt aattttgcgg
     9241 tatattctgc aaggagacga ttgtttttta aatttagttc aaatgaacgc gtttcttttg
     9301 attcataact aatgtaatgt taagtttaaa ttgtttagaa tgattttaaa agaatattca
     9361 aggaggtttc atcaatggaa aaaatcaaag ttgtcattgt aggtgcatct catggagggc
     9421 atcaatcgat cttggaacta ttgacacgat acggtgaaaa tgtagatatc acgctatttg
     9481 aagcaggaga ctatatttcc ttcatgtcat gtggtatgga gctgtattta gaagacaagg
     9541 taacagatgt aaatgatgta cgtaatttca gaccagaaaa ctttccgcaa tccaatgttc
     9601 atattttaaa taatcacgaa gtaaaaacga tcgatgccga taaaaaaaca gtaacggtta
     9661 ctagcgcatc agataacgaa acaaaagaat atccttatga caaattgatt ttgagttctg
     9721 gtgtcaaacc aaacaatctg ccagtaccag gagcagatct agaaaatgtc tacttgatgc
     9781 gtggatacaa ttgggcaacc aaaatcaaag aaaaattaaa tgatccggct attaaaaaaa
     9841 tcgctgtcgt aggtgcgggt tacattggga tcgaagcagc agaagcaagc cgaaaagctg
     9901 ggaaagaagt cgtattgctt gatgtgatcg atagaccttt aggcacttat ttagatgctg
     9961 agatgacaga tattttagaa caacacctaa atgaaaacgg tattgaagtt ttgacaaatg
    10021 caaaaatcga agcttttact ggtaacggaa aagttgaggc aatcaaaaca agtgaaaaag
    10081 aaatcccagc agatctagtc attcaagctg ccggtgtgaa ggcaaataca gaatggttaa
    10141 aagggatcgt cgacttagat gaacgtggct ggatccaaac aaatgaatac ttgcaaacga
    10201 atcttccaga tgtgtatgca gtaggagatg ctacacttgc ttattctatc ccagcacgca
    10261 aaaaaatgcc gatcgcttta gcgactgttg cacgtagaga agcacgttat gtagtaaaac
    10321 atctttttga agaagtacca aacaaaccgt tcggcggagt cgtaggttcc tctgcattaa
    10381 gtgtctttga ctatcatttt gcagcaagcg gattgaatag ctttacagca gcaaaagcag
    10441 gagtagatgt acgagtgtct tattatgaag atacgttacg accaaaatat gtaccagaag
    10501 aacaaggaaa tccaaaagtg tccatccaat tattctcgga tccaatgacc catcaattgc
    10561 taggaggagc agtattatct acctatgatg ttacaggtca aggaaatgta ttggcactag
    10621 cgatccaaaa caaactgact ttagaagaac tagcagaagc tgacttcttc ttccagccag
    10681 gctttgaccg acaatggagc atgttgaatc tagccgctca acaagcgtta ggagaagaac
    10741 catttgtaga ataagaaaac gaaatatttg aaaagaggtt gtgaaaaagc tgccttgcat
    10801 caagtaataa gaacagccaa gtaaaaacag cttcttatgt ttttaacttg gctggactta
    10861 ttaccgcaga tgcaagcttt tgaacaccgt ttattttcaa aaaagggact gtgacacgat
    10921 ttgtgtaccg acccccaaaa gttagaccaa aaaatctaac ttttggaggt cggtattttt
    10981 tatggttaaa tatacttttg aacttaaatt aaagatcgtt catgattatt tggatggcaa
    11041 aggcgattgg accgtaaaaa tccagatcaa gagattgaag cggaaatgct taaaattcgg
    11101 gagaagcaca aggattatgg ctgtttacgg atgaccaatg aactaagaaa ccgcggtttc
    11161 tctatcaata aaaagaaagt tcagcgcttg atcaagaagc taggaataaa agtaacggca
    11221 tacacgcgta aatcacgtcg ctacaattct tatcgaggaa aagttggagc agtagccaaa
    11281 aatcggatcc atcgtcgttt ctacaccagt atctgccatc agaaaatcac gacggataca
    11341 actgaattta aataccatga agtggatgcc aaaggcatta tccgtcaaaa gaaattgtat
    11401 ctagatcctt ttatggatat gttcaatagt gagattttat cctatcgtat ctctgaaaag
    11461 cccaatgcat tagcggtaat ggaaggtttg gaggaagcta tccagaccac taacgattgt
    11521 ccctatcgtc gtacgtttca ctcagatcaa ggttgggcgt atcaaatgaa agcttataga
    11581 aacaaattga aggagtacaa aatctttcaa agcatgtctc ggaaagggaa ctgcttagat
    11641 aattcactta tggagaattt ttttggctta ttaaaacaag agatttttca cgggaaagtt
    11701 tataactgtt tcgaggaatt aaaatcagcc atcgatagct atatctatta ctacaataat
    11761 gaacggataa aacagaaatt aaactggcaa agtctcgtcc aatttcgtaa aacaaccgta
    11821 acggttgtgt aaaaatatca ttttagagca taaaaaaaag gagtggattt ctccactcca
    11881 aaaaagtcta acttttgggg gtcactacag tgtcacagtc ctttttttag taaaaggata
    11941 catttcttcc ttgtatattc catgcgtgtt cgtgagaaag catatataat ttaattaaca
    12001 gatagttagg gggagaaaaa atgatttttt caattacaat gaaactggtg ttcgggttga
    12061 tcggtttatt attagtggta cgtttgcttg ggaagaaatc catgtcagaa atcacaccgt
    12121 ttgatttagt ctatacgctt gttttaggtg ggatcttaga agaatcaacc tatgatgaca
    12181 atgtccatgt agggcatgtc ctatttgcca ttgcgctttg ggcagtaatg atttatggta
    12241 tcgaacgtat cgtccaaaaa aatgaaaagg tcaatcgttg ggtcaaaggc gagccatctg
    12301 ttctaatcaa agacggcgtc atcaacatga cagagttgac aaataaccat atcgagatgg
    12361 agcagttgcg agcaatctta agacagcaag aatgcttttc tcttgaaaat gccaaacatg
    12421 ttatcctaga gaatgccggt caaatgagtg tattgaaaaa atcagatgaa gacaaagctt
    12481 tatcgatcct gcttgtagat gaagggcaaa ttcagcataa agtgcttcag tccaatcaat
    12541 tgactgaagc atggcttatg gagaacttga agaaagaagg ctatgcagat gtaaaacagc
    12601 tgatttatgt tgaatggtca gaagaaaaag ggctctacat actaaataaa gaagacgtca
    12661 tgcatgaatc ttaccgaata gatggataga tagacagagg ttgtgaaatc agcgccttgg
    12721 cttgttactc aaaactaagc gactttttca caacctctga tatctaatat acgttgttcc
    12781 agaagttgct tcttcgaaaa ataagccaaa attccacaaa aatttaagaa gcaattttcg
    12841 aaaaatcctt tcttattttt tgaagttgat cacttctgtc acaacctctc atgtcacaaa
    12901 ccattttttt gtttccttta actcaatata ccgtacgtct tcccacaata tcggctgatg
    12961 gtcaatcata aagtaatctt cataggcttc accaatgaag tgcccttcga cctcatccaa
    13021 gagtcttcca aattcatctt ttaaattgag ctgaatgatt actgctttac gagataaaaa
    13081 ggcttctgtc agtatactat cgatttcttg caatgtcatt tgttgtttag gaagaggctg
    13141 tttgcgtgct tcttcttcat tttttttgat accgttgact agttcttcca tcgcaaaagc
    13201 agtcccccat tttaatccaa atggccgatc tcgatactcg ttatagtcaa tgaatttttt
    13261 tgctgtccgt ctcatctttt ccaccttcta atccactcat accacctgca tgcccgccaa
    13321 cgagagaact acgtgcgatg gcgcgtcctc cttgcaagag actatgcgca tggatgattg
    13381 attgaaagcc gtattttttt ctaatcgtat cgacaacaaa atcaactttc gcttcattta
    13441 tctgcttttc aggatcttca aaaaggttaa gctgtaaacc tgtcgcgtat acgaggtcgg
    13501 aacagttcac gcctacacgc cgaatatcct ggtagtggta atgctggtgg aaaatggtca
    13561 ataaagcatc tgctagatct ctgctagcat ttgtcgcttc gattttcatt tgctggtgaa
    13621 acccggtctt gccatttgga tcaagatagc ctaaagagta tcctacccat aagctgacta
    13681 cttgtgtttt tgctgcagat cgtctcaagc gagtgccgac ttggtcggcc atttctttga
    13741 taacgatctc gatttcttct cgtcttgtat aatctctatc taggatttga ctatttccga
    13801 tactttttga ttttggtgta tattcttgtc ccaaaaaact tcgatcgatg ccccaagcat
    13861 gggcataaag ctgagtaccg atgaccccta gcttgctttt caagtgataa taatccgcgt
    13921 gagctaaatc ataaacagaa tgtataccca tttgtttcag acgttttgct gttcgtttgc
    13981 cgattcccca aaagtcagtg atatctgaga ttttccataa cttttctggt acatcttcat
    14041 aacgccattc agcaaacatt tgctttccgt cttttgagcc attgtctaag gctagtttag
    14101 caagtagcgg gttatcgcca attcctatag tcgtgtagat tccggtctgc tcaaacacat
    14161 ctttatggat cagtttagcc atgtcgtacg catttgtttt accaaagagt gccatgctat
    14221 ttgttacatc aaggaagctt tcatcgacgg aatagacatg atggttcgct tcgtctgcat
    14281 attttttata gatagaattg atttctctgt tcttttccat ataataggcc atccttgggg
    14341 caacaatata taaatcactt ggatagggga agggaagatc tctggcacgg ctgatattgg
    14401 tgatgccgta agcttttttt gctgcaggac tggaagccaa gattaatccg ctaccccgtg
    14461 tttctgtcga atcggacgga taggacatga ctactaattt agttgtaagg ggatcaagat
    14521 ccttttcaac gcactctacg gaggcgtaaa aagatttgca gtcaatacat aaaatatctc
    14581 ttgaaggttc tttttcataa acaaataccg gattttgatt cgcccacata acaatcacct
    14641 cttcaacaca attagaacac acgttctctt tccgtgtcaa taaaaaacag caattttcaa
    14701 atacgagcaa aaggctcaca tttggaaatt gctgagaggt tatttttatc tatttgtttt
    14761 tagctgagat cgttgatttt tttatcaaac atgaacagct tctggatcga tctttgttgt
    14821 cccatttcca atgtgtgcaa cataaaattc cggagcatag ccgacaactt tcatgtattc
    14881 atcatagaca ttgtttttga aggcaggtat attttcttct ttgacaagag caatcgcaca
    14941 gccaccaaat ccagctcctg tcattcgtgc acctaagaca ccttcttgtt tttgagcagc
    15001 atctactagt gtatctaatt cgatcccagt tacttcataa tcatagcgaa gagagtggtg
    15061 agaatcattt aacagttgac caaatgcttc taagttccca gcttctaatt cagcttttgc
    15121 cttcaaagtc cgctgatttt cagaaacggc atggcgagct ctgcgtatca atgtatcatc
    15181 gccgatttgg tcaatagttg attcaaattc ttcttcatct aagtctccta aagaagagat
    15241 aggcaaagtt ttttgcaaac gtgccaaggc ttcttcgcat tcgctacggc gttcattgta
    15301 ttttgaatct gccaattccc gccgcttatt cgtattcatg atgacgattg cataaccatc
    15361 tagtttcact ggaaccattt cgtatttcag cgtatttgta tctaataaga ttgcatgatc
    15421 tagctcaccg aagccgatgg caaactgatc catgatacct gaattgacac cgatgaattc
    15481 attttctact tttttacctg tttggactag ttccaagcga tctgttttta aatcaaacag
    15541 atcttctaat acaacaccta ctaataattc taatgaagcg ctactggaaa gtcctgcacc
    15601 attagggatt gttccttcca ctaacaactc aaacccatga tcgattgagt gtccagcttc
    15661 ttttaattta aggaccattc cttttacgta atttgcccag tttgcttttt tatcatattc
    15721 tagctcatct aaagtaaaac taatgacacc gatatcgtca aagtttgtgg aataaactaa
    15781 tattttatta tcgtctcgag gtgctgctac accttgcgtt ccataagtga tggaagcggg
    15841 gaagacatgg cctccattgt aatctgtgtg ttcaccaatc aaattgatgc gtcctggtga
    15901 gaagtattgt gtagtttttt taggtccgaa tgtttcggca aattttgtgt ttaagcttct
    15961 tttgatttcc atgatgagtt attcctttct tgttttgatt ttgaattctg tcactgtttc
    16021 aaatactgat ccggctttta aatgaatcga accaaatgct gagaagcgct ctgcacctgg
    16081 agctgtttga gtctcaaatg tgatgccacc gtgatgtgcc agttttcggt tgcgcatttc
    16141 tggtgtttct gtgccgaaat ttgcagtgaa aataaccaca ctagaagcat ctgtagcaat
    16201 acctacttgg attcttttat cggggctagt caagcgagcg gcttctttac ccaagccggt
    16261 ttcttttaag aaaaatggat gatcgattcc gtcaacaaga tttttttgat ccaaatcact
    16321 agcaaaaacg gacgctaatt ttttaggtat ttgaaaatcg aaagcacttc ctgcagcatt
    16381 ttcttttacc cctataggaa tactatctgt tcgcaaagga gcataagctt cgctattgag
    16441 ccaaagctca tgttgatcga ttgactgaga agcatctccc gtcaagttga agtaaacatg
    16501 atttgtcgga ttgaataaag tatcttggtc actggtgccg cgagaggtca ctcgccagat
    16561 attatctttt gtcaaagtat agcggatttc cacatccatt gtacctggaa aaccatgttc
    16621 tccgtcggga cttgtagtag taaaaatgac agaagcttca ttttcgccat tcaaaatgac
    16681 ataagaccat ttttttaatt caaatccagg agcaccacca tgcagattat gtccagtttt
    16741 tggatctgtg gctaattggt aagtttttcc attgagagaa aagcggccat tttcgattct
    16801 ccctgcggtg cgaccgatag aagcaccgat atacggatct ttttcgatat attcttcagc
    16861 ggtgtcaaat cccaaaacta attctcgatc atttgatttt aaactgacga ttctagcacc
    16921 taaatcagag atagaaagtg ctagcttgtt tttgttctcc aaggttatca agtgataacc
    16981 ttggccaaaa tctttttctg taatcttcat tttcaagatc ctttcttaat gggaaggaac
    17041 tactgcatcg tgaagttcat cttccattgt atcagctgga gccaatttgt tttccaattc
    17101 ctcaacgatt tgtgcatgtt ttttctctgt caatgtaatc ttccaagcaa aaataatagc
    17161 ggagaaaatc atcaaagcag caggagcata gaatgcataa gctttgaaag tcgttacgcc
    17221 atgagcagtg atatcagcag cagtagcatt accagtcatc ccagaagcga tagcgacaaa
    17281 ccctacgatc ccgttagaca aagcaccagc gattttatct agtaaagggc gaattgatag
    17341 agtaacagct tcgttacgga taccattttt ccattgacca tattcaacag agtctgtgat
    17401 cgtcatcaaa gcggataaga aaatcaattg ttgagggaag tagaacaata ctagcccgat
    17461 gataacgatt ggcaaacttg aacctgcgat cgtaaagaaa aagtaagaag aaatcatcat
    17521 tgcgatccct ccaaggtaaa cgtagcgacg agagatgacg cgtgtaagca ttgggaatag
    17581 tggaacagct actaaaccaa gtaaggcagc aactagacca acgatccaaa attcagttgg
    17641 ttggccaagt acatatttga agaagtagaa taatacggca gttgttgcta cattggcaat
    17701 cgcataagcg atataagcaa gggcaagcca catcaactga tcattttgag caactgcttt
    17761 gaacacatct ttgatcccag ctttttcatt ttgctgacgg atcgcagtat cgacttcttt
    17821 tgtgcccaag gcagtgataa tcgcagtacc gcctgaaata acagcagcaa tcacaccgaa
    17881 ccagaaccag cctgaagcgc cttcaatatg tttatgagta acaagatagg taaagaaagt
    17941 aacgataggg acaacgacta gagtagtacc atttgcacct agagaagagc cgaaacgagc
    18001 aaacgtagca aatttttcac gttcctttgt ttccgtactc aaagctggaa tcattgacca
    18061 aaaagcgata tccttaaatg aataaaaact atccaataag atgaaaacga ttgcaaataa
    18121 tatgataaat aaagtcgtat tatgagtagc taagccaaag aaattggtaa agattgctac
    18181 caagagtacg gaactaacaa cgccgccgat aactaaccat ggtttgaatt taccgtattt
    18241 tgtacgagta ttatcgatga tgccaccaat caaaggatca aaaataattt caactaagcg
    18301 gatacctaca actaagcttg taactgtagc aatcattttt gcattcgttg ccttatctgt
    18361 tcctgcgaac atctggcttg tcacgaatac cataaaataa gtgcttaatg taacatagaa
    18421 tgtgtcgtgt ccaaaagcgc ctaaagcgta ggacacacgt tggatcattt tgcttttcat
    18481 aatctgtatt cctctcctgt tcaatatata atactaagta aaccggttac aacgaataac
    18541 aaagatcttt taatgactta aacttaacaa gaaatcgctt cctttttaat atatttctgt
    18601 gacttttatt taactttttg ttgcattata ctaaaggagt aaggagggat agacgtggag
    18661 caatcgattt ttttaaaaaa ggaacatcaa caagtagaaa aagaattgta ttttgatttt
    18721 tgtggtttct ctaaaacgat gccgtttcat tcttttggtc ctgctattcg caatgattac
    18781 atcctgcatg tcgtgatgga aggcaaagga tcttatcatg tgaaaaatca acaattccaa
    18841 ttgaaaaaag gtgatctgtt cttgattcgt ccaggagact cgacgtttta cttatcagat
    18901 ggagaagatc cgtggatgta ttgctggctt tcatttggtg ggagtgctgc agaagaaatc
    18961 attcggcagt cgcttttcag agaagaccaa tacacgatgg tctcttctga catacaaaaa
    19021 tatatcgaca ttattttaga atgcatgaaa gatacggaag aaaccttgga aaatgaatta
    19081 cagttaacag cattaaccta ccgtttttta tcagtccttt taaaagatgg cggaaaggtc
    19141 agcggtggtg tacaaaaggt tcattcctct ttagctgttg aaacagtcaa atacatcgag
    19201 gcacattatg ctgaaaaatt gtctgttgag gaaatcgccc agcatttagc ggtcaatcgg
    19261 agccatttgt cgcgagtatt caaaaatcat atgggtacca gtatcaagga atatttgatc
    19321 ggggtacgga tcaatcgagg ggcgtttctt ctttctctga cggatgaatc cgtggaaagt
    19381 attgcctatc aagtagggtt caatagtcta gtcgtattta gccgaatgtt caaaaagagt
    19441 accggagaaa cggctacgaa ctatcgtaaa cgtatgaaaa acgaaaattt taagaatatt
    19501 gctttggata ccttgaaaaa acagttggaa aaacaaaccg cagtttcttg gacaacctga
    19561 ttagtctggt taaaatgaca agcatcggca aaagaaactt gtggcggtta tgctacactg
    19621 taaagcgaag actaaaaagg aggaaaaggg atgcgtacac ttatcatcat tagccatcca
    19681 acgatcgaag aatctttaaa tcagcaattt ttcaaagaag cttctgctgg tttaccagct
    19741 acttggcatc atttggaatc aagttatcca gacggaaaaa tagatataga gactgaacta
    19801 cagctcttgg cccagcatga tcggatcatt ttccagtttc cgttttactg gtatagttct
    19861 cctgctcatt taaaaatttg gcaagataat gtcttggaac gagcagcaca tgtattaaaa
    19921 gggaaagaac taggaatcgt attgacaaca ggtgtggcgg aaaaagaata ccaagctgga
    19981 ggaaaagaag aatatacgat ttccgaattt ttgaggccgt atcagcgaat cgcaaataaa
    20041 ttccatatga cctatcttcc gccattcgtt ttagcacaat ttatgtattt atcccaggaa
    20101 aaacgttggg agaagctaat tgcttatcag cagtatctct cattggaagg caaaccatcg
    20161 ctgactcagc ggattgactg gtttattcaa agagtacagg aaaatcagaa aatgcaggaa
    20221 gaagaatctg aaaaacaagc atatatcgta gaagcactga ctgaggcaaa agagcagatc
    20281 gaagacttga cctttacact gcaagaaatg aaaggaactt cattatgaca aatgattggc
    20341 tgatccaaca attagatgaa ttaagcgaaa aatcagaata tcaagaccgt gcgctcttat
    20401 atgaaataaa gaaactgatg gctgagatag acgaacgaac aatgcagctg caaggagaaa
    20461 tcgacggaca atcttggaat catctcaact ggtgaaattt tcttataaat aacaaagata
    20521 aaaatgtgca cagcgacaat tatttgctac aataagaata tgaagcgttt gataacgcta
    20581 ttatacataa aaattggtgg tgaaaaaaat gtatgatgaa aatgagtggg gagaaaatgt
    20641 tcggatgacc cgaagaagac agcaaaaaca ggcagctcgt aagcggatga tcatgattgg
    20701 ggcagcagtt gccgtgtttt tgatcatagc aggaggagtt gcgggtctat attttcatgg
    20761 aaaaaattcg tcagaagcga aaagtacgac cataacggaa acaacgacaa agaaaacaga
    20821 gacgaaacaa acaagtgagt catcaactaa aacaaaagag acacaaactt ctgaagaaaa
    20881 aacagcaaat ggtttgccag agcaacttca agcaatcgta aatgattatt cgaaaaagat
    20941 cgaagaaaag acgcctgcat tgattgaaga ataccagaca gaaattcagg gaaatcaaga
    21001 gggaatagct ggtttatcag cagttgctaa ccaaaaagca cgtgagcttc aagcaatctc
    21061 cgacgaaggg atcagaaaat tacgtgcagc gtatcaggca gcagaaaata aagacggtgt
    21121 agacttagat actttgatca atcaattatc ggctaattat acgaatcatg tcgctaagat
    21181 ttccgatatt tatttgcaga ctagcgcctc tcttcaagca gagtcaacaa gcacacaaga
    21241 cacaacatct tctgattctg agacagcaga gtcggtagaa gcttctgaaa tgcaagctcg
    21301 aacaacacaa gacacaacag attcctctga aactaatacc aacagcgcga cttcacaggc
    21361 tgcgacaacc gtagttttgg aaggagaagg tccaaatcaa atagccgaac gaactggcgt
    21421 accagtagaa acgatcttgt cgctgaatgg gatgagcatg gagaattatt tcctgactcc
    21481 cggtgagacg ctaaaattga attgataaaa gcaataaaaa agaacgaagc ttccactaag
    21541 gtgaagcttc gttctttttt tattttccgg ttcttttttc gcgtacaaag taatggatcg
    21601 tcatgtaaag caagaaaacg agtccaataa tcaatgagat tctcgtttca ggattgataa
    21661 acatgaaaat caatacgata gctaaaaaag caagtgtcac ataattcgta taaggagata
    21721 gtggcatttt aaaaggatga tcatgcattt tatctgcgtg cttctttttg aagcgaatct
    21781 cgctgatcaa aatcactacc caaggaacca taccaggcaa aatacttgag ctgtatacga
    21841 aaacgaaaat attttctgac tggttgatga acattggtag aagataattt aagatcagac
    21901 caattaagat tccacctgag attgctacga caggataaaa aggcgcaccg tgacgagata
    21961 gtttcagaaa ttttttagac atctttccat ttatggcaag tgtatagacc atacgacttg
    22021 cgctgaaaat ccctgaattg caacctgata atgccgctgt aatcacgaca aaattaatca
    22081 gtcctgcagc aaatgtaatg ccgactttag aaaaagtttg tacaaaaggt gaaccaatct
    22141 cactcaattg attccacgga tagatactca caatgataaa aatagcacca atataaaaaa
    22201 tcaagattcg accgatagtg gactggacag cttcaatgat cgtcttttta ggattctctg
    22261 cttcaccagc agtcattccg atcagttcga ttccttgata ggaagcaaca acgatcgata
    22321 atgcaaagaa aaatcctttg acgcctccag tgaagaatcc accatttttc caaagatttg
    22381 aaatccctac tgcctgacca tgattaccaa atccaaataa gattacccca agacctgcaa
    22441 tgatcatcaa aatgatcgtt atgactttga tgatcgaaaa ccaaaattct aattcgccaa
    22501 acgccttgac agaaataaga tttgccagca taagaaacaa gacgacaatg attccgggaa
    22561 taattgccgg caaatcaggc caccaataat tcatataaga tcctacagca atcacttcac
    22621 ttattcctac gacaatatat tgaaaaatat tactccaagc ggtcaagtag ccagccacag
    22681 gatggatgta ctcgctcgcg taatttgcaa aagaaccagt cgaaggatca acgtaaagca
    22741 tttcgcctaa agcacgcata atcaaataca aaaagaatcc tgcaatcgca taggctccca
    22801 tcactgaggg ccctgtccaa cgtatggttg aggaggcacc cataaacaga ccgacaccga
    22861 tcgttccacc tagtgcgatc atttggatat gacgggcact aagacttcga gttaactttt
    22921 tttcttccat acaaaaactc ctttcgttgt tgagtcaaca agaaaaaata atcaaacgtt
    22981 tcaacacatg tttttcatta ttaggttagc cttattgaac tcttgatgtg attctacaga
    23041 aaattttcat aaaatgcgag gaaaattcga aaaaaacttt ttcatcattc tacttgaaaa
    23101 tgctttccat tttagttaaa ctatatttaa ataaaaagtt aggtaaacct aaaaaggagt
    23161 gtaagatttg gaacgtacgg ccattgtaat tcaagacctt tcagccgctt atcaagggaa
    23221 aactgttttg aaaactatca acttgacgat aaatccacaa aaaattaccg gtattatcgg
    23281 accaaacggt gcaggaaaat caacatttat gaaatctttg ctagagttga taccagctac
    23341 tactggtaaa gtcacctttt ctggacagcc ggtaaactca gtccgtaaaa aaatagccta
    23401 cgtggaacag cgaagtgaat tggatctttc ctttccaatt gatgtattag gcgttgtact
    23461 tttaggaaca tatccatctt tacgaattgg acaaagacct gggaaaaaag aaaaagaacg
    23521 tgcaagacaa gctttgaaaa aagtagggat ggaagaatat gccaaaagac agatcagcga
    23581 actatcgggt ggacagcttc agagagtttt tattgcaaga gctctagccc aaggagcaga
    23641 atggatcttt ttagatgaac cattcgtagg gattgatgcg ttaagtgaac gaaagatctt
    23701 tgacatcttg caggaattga agaattcagg aaaaacgatt ttgatcgtcc atcatgatct
    23761 tcataaagta gaagaatatt tcgatgaggt tattctttta aataaacagc tgatcgcttc
    23821 cggtccagta caagaagtct ttacatcgaa gaaccttcaa ttggcctatg gtgagatcat
    23881 cagacattta gtaaaaggag gagagaaaaa atgatccaat cctttattga tggattaatg
    23941 aattatcaat tcctgcaata cgctttggtc acttccatgc ttgtaggact tgcttctgga
    24001 gtcatcggtt cttttattat tttacgaggg atgtctttga tgggagacgc tatctctcac
    24061 gcagttcttc caggtgtagc catttcttat atgtttggtt ttagttacat ctttggagcc
    24121 actgcttttg ggatgctaac agcagcagtg attggtttcg tgactcagcg gagccgattg
    24181 aaaaatgata cagcgattgg aattgtcttt agctcctttt ttgcattagg gattatcttg
    24241 atttcatatg cccagagtgc gacagatttg tatcatattt tatttgggaa tgtcttagct
    24301 gttagggaaa gcgatttgtt gctgacagca ctcgttagtg gaatcgtctt gatatttgtc
    24361 tttttctttt ataaagaatt gaagatcaca tcattcgatc cgacaatggc aaaagcttat
    24421 gggctgaaca catcgctgat ccattatctt ttgatgttct ttttgacatt agtggctgta
    24481 gtcagtttac agacagtagg aacaatcttg gtgattgcca tgttgatcac accagccgcc
    24541 acggcttact tgctaacgaa ccatttactg aaaatgatca ttacagctgc aggaatcggt
    24601 atgctaagtg cagttgtcgg tgtgtttttc agttatagtt acaattggcc atcaggagct
    24661 acgatcgtgt tagcatgtac cgcatttttt atccttgctt ttttattttc tccaacaaaa
    24721 ggaattttat ttcagaaagg aagcagatag atgaaaacaa aaaaatcact ctttttaatc
    24781 ttggcagtta gtttcttggt attagccggt tgcggaaagc aggcttctga ccaagcggat
    24841 gaaggaagta aagaaaagtt gtcagtcgtg gctaccaatt cgatcttggc ggacatggca
    24901 aaagaggtcg gaacagatca aatagatatc cacagtatcg taccagtcgg aacagatccg
    24961 catgaatatg aagtactacc agaagacatc aaaaaggcaa gtgatgcaga tgttatttta
    25021 tacaacggtt tgaatcttga aacaggtaac agctggttcg ataacttgat ggaaacggct
    25081 aaaaaagaag aaggaaaaga ttattttgca gttagcaaaa atgtagaacc tctttattta
    25141 actagcggtg aagaacatac aaaagcagat ccccatgctt ggctagacct atctaacgga
    25201 ataaaatatg tggaggaaat cgcacgtata ttctctgaaa aagatgcaga aaatgcgaca
    25261 ctctataaaa aaaatgcaga agcatatgtg gaaaaactaa aagaattaga tacacaagcg
    25321 aaggaaagtt ttgcttctat cgaagagaac aaaaaattat tagtaacaag tgaaggggca
    25381 ttcaagtatt tttcacgagc atatgatttg ccagcagctt atatatggga gatcaataca
    25441 gaaagtcaag gtacgcctga tcaaatgaaa gcgatcattg atcagataag agcaaaagaa
    25501 gtaccggttt tattcgtgga aaccagtgta gactcaagaa gcatggaacg ggtagcaaaa
    25561 gaaacgggtt tgaaaatcta tgataaactg ttcactgatt ccatagcaaa agaaggagaa
    25621 caaggagatt cttactatca gatgatgaaa tggaatattg aaacgatcca tgaaggtttg
    25681 tcacaaacaa aagaaagctg aaaagtgcaa ggtctagctt gaggagcaga ccttgcattt
    25741 tttataagag atatgaaaaa aaattatgct gtttttcgtc gtacagatac cttttttgaa
    25801 gaaattccgt tatagttaga gtaaggaagg aaggtgtggc tatgaatttt acacgtaaag
    25861 ataaatatac gattaaagaa gaagtctttc gattgatcag ccagcaggaa aataccgaca
    25921 aggatcaaga ccttcaattt cttgaagcag aattagctaa attattcata gcaaatcaaa
    25981 atgagacaac agaaccagat gaagagctgt acataggcaa atggttgttt gatgaatttc
    26041 agcctttaga tgaaaatacc ggtatcggtt attttgtgtt cctttctcca gtcggcttca
    26101 cacctggcgt attagttaaa caaaacgacg aaataaccgt caaagcatgg cctgcattat
    26161 tttcagtcat caaagcagaa ggagtaatca aaaagatcca gtcggcctat tataaaagtc
    26221 aatttattgc caacagcaaa ttaatgaata tgttttataa tcgtctagta gaaattgcta
    26281 ggcctgggaa aatcccgcaa acattcacgt acacacagca aaaaaatctc tatgaagaag
    26341 tattgtattc ccgcgatctg tctgagtttt cgccatggga agaggtaagg ctccatgaac
    26401 tgttaagtac aaaaccagtt tcattgaatt tttttaatgc attagataac aaaagaaaat
    26461 taatttcaac aaattattgg gaagtatttg ctgcaagaaa aaaatggatg gaagaaaaga
    26521 agattgccga tcagcagatc cagtttgtcc agctgaaaaa agaaaaacta tattatgggt
    26581 tatacagccg ttaacagcaa gcgaggaaga ccatgacatt aaaagaagaa atcatacaag
    26641 aaagcaaacg attaggaatt gacaaaatcg ggttcaccac ggcggaacct tttgattcat
    26701 taaaagaatc gttggaagaa caaaaagcag ctggccatac ttctggcttt gaacatccaa
    26761 atatcgatga gcgtctttat ccagaaatga catttgatca gccgaaatcg atcatttcca
    26821 ttgcacttgc gtatcctact aggatccatg aagaagtgcc tagggatgaa aaacgcgggc
    26881 agtttgctcg tgcttcttgg gggatcgatt accatgatat actcagagac cgtttgaacc
    26941 gattgatcga attcatccaa acaagagcag aaaaatggca aaaagaagag gaatggcgtc
    27001 tagctccgca agtagatact ggagaactga tcgatgtcgc tgtagcacag cgagctggtt
    27061 taggatttat tggacgaaac ggattgttga tcactgaaga gttcggttca tttgtctatc
    27121 ttggtgagat cgttactaat attgtatttg agcctgataa accaggagaa tttggttgcg
    27181 gtgactgtat gcgctgcgtg actgcttgtc cgacaaaagc attgcttggt gacggaagaa
    27241 tgaatgccca gcgatgtttg tcttatcaaa ctcagacaaa agggatgatg ccggaagaat
    27301 atcgtaaaaa aatgcgaaat gtcatttatg gatgtgatat ctgccagtta gtctgccctt
    27361 acaatcaagg aaaagatttt cattttcatg aagagatgga accaaaagta gacgaagtat
    27421 atccaaaatt aaagccaatg ctttcgatgt ctaataaaga cttcaaacag caatttgggc
    27481 atttagctgg ttcatggcgt gggaagaaac ctttgcagag aaacgcattg attgctttag
    27541 ctaatctagg tgatcgaacg gcattgcctg agatttcaga atgtttaaca gatgtccgcc
    27601 ctgtcatcag aggaacagca gcttgggcta ttggacgtct aggaacaaaa gaaccggaaa
    27661 aatggcttga tgtattagat gaaatattag ctaaagaaac agaagaagtc gttataaacg
    27721 aattggaaca tgcccagaaa ttattgcgga gaaagttaaa ataaaaaaat agttaacatt
    27781 attcaaa
//
LOCUS       DACDPS010000030        26861 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.030, whole genome shotgun sequence.
ACCESSION   DACDPS010000030 DACDPS010000000
VERSION     DACDPS010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.030"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(45..392)
                     /gene="rbfA"
                     /locus_tag="IWJ07_001392"
     CDS             complement(45..392)
                     /gene="rbfA"
                     /locus_tag="IWJ07_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAP9332980.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(419..2725)
                     /gene="infB"
                     /locus_tag="IWJ07_001393"
     CDS             complement(419..2725)
                     /gene="infB"
                     /locus_tag="IWJ07_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAP9332981.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(2738..3049)
                     /locus_tag="IWJ07_001394"
     CDS             complement(2738..3049)
                     /locus_tag="IWJ07_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAP9332982.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(3046..3339)
                     /locus_tag="IWJ07_001395"
     CDS             complement(3046..3339)
                     /locus_tag="IWJ07_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAP9332983.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(3361..4536)
                     /gene="nusA"
                     /locus_tag="IWJ07_001396"
     CDS             complement(3361..4536)
                     /gene="nusA"
                     /locus_tag="IWJ07_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAP9332984.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(4560..5033)
                     /gene="rimP"
                     /locus_tag="IWJ07_001397"
     CDS             complement(4560..5033)
                     /gene="rimP"
                     /locus_tag="IWJ07_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAP9332985.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(5177..9529)
                     /locus_tag="IWJ07_001398"
     CDS             complement(5177..9529)
                     /locus_tag="IWJ07_001398"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAP9332986.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            complement(9741..11450)
                     /locus_tag="IWJ07_001399"
     CDS             complement(9741..11450)
                     /locus_tag="IWJ07_001399"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAP9332987.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            complement(11518..12786)
                     /gene="rseP"
                     /locus_tag="IWJ07_001400"
     CDS             complement(11518..12786)
                     /gene="rseP"
                     /locus_tag="IWJ07_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAP9332988.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(12947..13747)
                     /locus_tag="IWJ07_001401"
     CDS             complement(12947..13747)
                     /locus_tag="IWJ07_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAP9332989.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(13744..14556)
                     /locus_tag="IWJ07_001402"
     CDS             complement(13744..14556)
                     /locus_tag="IWJ07_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAP9332990.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(14751..15308)
                     /gene="frr"
                     /locus_tag="IWJ07_001403"
     CDS             complement(14751..15308)
                     /gene="frr"
                     /locus_tag="IWJ07_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAP9332991.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(15311..16033)
                     /locus_tag="IWJ07_001404"
     CDS             complement(15311..16033)
                     /locus_tag="IWJ07_001404"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAP9332992.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAHMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(16169..17050)
                     /locus_tag="IWJ07_001405"
     CDS             complement(16169..17050)
                     /locus_tag="IWJ07_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAP9332993.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(17149..17931)
                     /gene="rpsB"
                     /locus_tag="IWJ07_001406"
     CDS             complement(17149..17931)
                     /gene="rpsB"
                     /locus_tag="IWJ07_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAP9332994.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            18290..18769
                     /locus_tag="IWJ07_001407"
     CDS             18290..18769
                     /locus_tag="IWJ07_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAP9332995.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            18986..20077
                     /gene="phnW"
                     /locus_tag="IWJ07_001408"
     CDS             18986..20077
                     /gene="phnW"
                     /locus_tag="IWJ07_001408"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAP9332996.1"
                     /translation="MYKLLTPGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSGSFGVESVLSSVIRPEDTVLICSNGAYGQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQEKDLL
                     FIVDAMSSFGGIPIRVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            20070..20855
                     /locus_tag="IWJ07_001409"
     CDS             20070..20855
                     /locus_tag="IWJ07_001409"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP9332997.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(21201..22892)
                     /locus_tag="IWJ07_001410"
     CDS             complement(21201..22892)
                     /locus_tag="IWJ07_001410"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAP9332998.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(23314..24261)
                     /gene="arcC"
                     /locus_tag="IWJ07_001411"
     CDS             complement(23314..24261)
                     /gene="arcC"
                     /locus_tag="IWJ07_001411"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAP9332999.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(24376..25395)
                     /gene="argF"
                     /locus_tag="IWJ07_001412"
     CDS             complement(24376..25395)
                     /gene="argF"
                     /locus_tag="IWJ07_001412"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAP9333000.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(25486..26715)
                     /gene="arcA"
                     /locus_tag="IWJ07_001413"
     CDS             complement(25486..26715)
                     /gene="arcA"
                     /locus_tag="IWJ07_001413"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAP9333001.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         7154 a         5800 c         4775 g         9132 t
ORIGIN      
        1 aatcccgcaa cccctgattt cacaggattt gcgggatttt ttgattagtc tttgttcaag
       61 tcgcgaagca attcatcgat atggttccca tagacaactg attcatcaag ttcaaagaac
      121 aattcaggtg tcttataaat acttagattg tgccctaact ctcgacgaat caatcctgat
      181 gctttattca atccatcttg tgcttttttc ttatctgatg ctaagtcaga taaaatgctg
      241 taatagatcg ttgcttgctg taagtccccc gtaactttca catctgtgat cgttacgttc
      301 tccacacgag ggtcacggac ctttttgcgc aaaatatcat tgacttcttt taagatttct
      361 tgaccgactc ggcgatcacg gtagttagcc ataatgattg gcctcctttt atcagttttt
      421 agtcattttt gacttcttcc atgataaatc cttcaattac gtcatctact ttgatgtcat
      481 tgaatttttc aatcatcgca ccacattcat aacccatttt gacttctttc acgtcatctt
      541 tgaatcgttt cagacttgca agttggcctt catagattac gattccgtca cggattacac
      601 ggacaccgct atctctacga atatatcctt ctgttacata ggcacctgca atcgtaccaa
      661 ctttagaaac tttgaaggtt tcacgaacga tcatttgacc ggtgattttt tcttcaaatt
      721 cagggtctag catccctttc atcgcagttt cgatttcttc gatggcttta tagatgatac
      781 ggtgaagacg aatatcgact tcttcagtat cagcttgcac ttttgcctgc ggtgtaggac
      841 gaacgttgaa accgatgata atagcgttgc tggctgctgc taaggttaca tcactttcat
      901 tgattgctcc gacagctgaa tggacgattt ttacacggac accttccaca tcaatcttct
      961 tcaatgatgc agccagtgct tctgctgaac cttgtacatc tgctttgatg attacgttaa
     1021 cttctttcaa ttcgccttct ttcaggcttt caaataagtt gtccaaagtc acacggcttg
     1081 tggcagcacg gttttccaat aatgcacgtt ttgcgcgttc ttcacccgct gcacgtgctg
     1141 tcttttcgtc ttcgaagact acgaaacggt cacctgcttg aggtacatca ttcaagccag
     1201 tgatttctac tggcgttgca ggtcctgctt ctttatcgcg acggccaaga tcgtttgtca
     1261 ttacacggac acgtccgtaa gtattaccga caacgattgg gtcaccaacg tgaagtgttc
     1321 cttcttggac aagtaaggtt gacacaggac ctttaccttt atcaagacgt gcttcgatca
     1381 ctgttccgat tgcgcgttgt gttggatctg cttttaaatc ttctacttct gcgactaaca
     1441 ggatcatttc cagcaattct tcgatgtttt ggccgaattt cgctgagatc tcaacaaaaa
     1501 tcgtttctcc accccatgct tcaggaatta attcatactc acttaattct tgcataacat
     1561 gctgaggatt tgcacctggt ttatcaattt tatttacagc gacgatgatt ggaacgcctg
     1621 cagctttggc atggttgatt gcttcaactg tttgtggcat gacgccatca tctgcagcaa
     1681 caactaagat cgtgatatct gtgatacttg ctccacgtgc acgcatactt gtaaaggccg
     1741 catgtcctgg tgtatccaag aaagtgattg gtttaccatc aatatcgatc tggtaggcac
     1801 cgatatgctg tgtgattccg ccggcttcgc cgcttgttac acgtgaatgg cgcaatgtat
     1861 ccaataaggt tgttttcccg tggtctacgt gtcccataat cgttactact ggcggacgtg
     1921 agactaattt atctggatcg acttcttctg cctcgaagaa tttatcgata tcagcaatgt
     1981 caacttggac tttttcttgt ggttccatgc catagtccgt cgctaacagt tcaatcgtat
     2041 ctttatccag tgcttggttt tgattgacca tgacaccaag cataaataat tttttaatga
     2101 tttctgctgg ctcacgatga atttttttcg caatatctgc tacgttcata ccttcagtat
     2161 actctaatac atctggcaat tcacggaatt tgcgtggtgg tacagctggt ttagttgatt
     2221 gttgctgttt gcctttttta ccttttttat tataacgatt acggttatta ttattaaatt
     2281 tgccgcgatt gttattgttt ggtttgttgt tattgttagt acggttttga ttgccgcctt
     2341 ggctcgttcc ttgacgattt tggctgctgg tatttcccgt attattttgg tttgttgttg
     2401 gtttttttcc ttgattcact tgaccgctcc cttgaccgcg atcttgataa ctgcggttat
     2461 ttttattttt ttgttgattg ttcgttgttt cttttttacg ttgatcgttc ggctgttgat
     2521 tggctggttt tctgcttgct tgttgtgcag cttgttgttt cgtgcttggg tttttgaacg
     2581 cttgttgcaa tttcttctca tcactgctag taatcgcacc catatggttt ttcacatcga
     2641 tgccaagttg ttgtgctttt tccacgacgt ctttactaga ttttttgagt tcttttgcta
     2701 attcatagat tcttttgttg cccatgcaat caccttccta accttcaatt agctccttgg
     2761 tcttcttcgc aaatccggca tccattattc cgaatacctt gcgaggtttt ccgatcatct
     2821 gcgtgatttc tgcttcagaa aatagctcaa agcaaggaac ttcataatat gaactcttat
     2881 cttttatctt ttttcttgta ttctcacttg catcggttgc cacaaacacc aattttgctt
     2941 tttgtttgcg gatttcagca atggtcagtt cttcacccgt gattagtttt cccgcgcgca
     3001 tcgctagacc gatgagattc atggcttttt gccgattaac tgtattcatt ttccaaataa
     3061 ctctttacgt gctttttggt gagtgacata gtcaagaagt tcttgataaa actcatctgt
     3121 taatgttgtt tcgagtactc gatcgagaat ttttttatcc caagcttgtt gtacttcttc
     3181 cggctcaagt gagacatacg ctcctcgtcc aggcattttt ccagttggat cgatcgatac
     3241 ttccccttct tttgaacggg tgattcggac catctctttt ttaggtttca tttcacctga
     3301 aaccacggat ttgcgtaaag gaattttcct ttgtttcatc tctttgcctc cttactaaag
     3361 ttaagcttct ggtttttctt cgaccgtttc attgttgaat gcaattgttt catattcatc
     3421 gtctgttaaa tctgactgaa cgattgcttc atcatgcagt tcttctgttt ctttttgttg
     3481 ttcttgattt tcataaaatt ccgtcatgtc agattctgat ttgatatcga ttttatggtt
     3541 agtcaattta gcagctaaac gtgcattttg tccgcgttta ccgattgcta gcgataattg
     3601 gtagtctgga acgacaactg tgcaggcctt tggatttgtc tcatcgaaga tcacatccat
     3661 gacttgtgca ggattcaatg catttgcgat gaatacagct ggatcttcat tccattcgac
     3721 gatatccatg ttttcgcctt ttaattcatt gacgattgct tgaactcgtt gtccctttgg
     3781 cccgacacat gtaccgactg catcgatatt tggatcattt gagcgaactg ccacttttga
     3841 acgatcgcca gcttcacggg cgatgctgac aatttcaacg attccatcat agacttctgg
     3901 tacttcttgt tcaaataagc gacgcaatag gtctggatgg ctgcggctaa caaatacttg
     3961 cggaccttta gacgtatttt ctactcggga aacataaact ttgattcgat catgtggttg
     4021 ataaaattca tttggcattt gatcttgttt agaaagaact gcctcaattt taccaagatt
     4081 gacataaata taacgtttat cctggcgttc gacgattcct tgcatgatgt ctttttcata
     4141 agcactgaat tcgttataaa taatcgttct ttctgcttca cgtacacgct gtaaaataac
     4201 ttgtttagcc gtttgcgcag cgatccgtcc aaaatctttt ggtgttactt caaaacggat
     4261 cgtatcaccg atctcatatg ctggattgat taaaagtgca tctttcaatg agacttccaa
     4321 ctgtgaatcc atcacttctt ccgttacttc ttttaccgca tagacatgga tctttccttt
     4381 tttctgatca aattccactt cgacgttttg tgcttggcca tagtgacgtt tgtatgcaga
     4441 aaccaatgct gcttctaaag catcgatcac gatttctttt gagatccctt tttcagcttc
     4501 taaagcgtct aacgcattta acatttcttt actcattttt ttctgattcc tctccgtgat
     4561 taaaattgga tagctaagcg agcttttgca atattttttc ggtcaaaagt caattctttt
     4621 tctcgtgttt ttatgcgtat tttcaatgta agctgttcag catcaaatga ttgaagaaat
     4681 ccttcatatt gtttttcgcc atctactggt tggtataagg agacatggac ataatcacca
     4741 agtgcctttt catagtcgct ttcttttttc aatggccgct ctgctcccgg ggaggatacc
     4801 tccaggaaat aagcttgagg aatcggatct ggatctgctg catcaagttt ttcactcagt
     4861 ttctcactga caaaggcgca ttcttcgata tcgataccgc cttctttgtc aataaatact
     4921 cgtaagaacc agctttttcc ttcttttaca aattcgactt cgacaagttc aaaattttgt
     4981 tcatctaaaa tcggtgtcac catttctgtg actgtttcaa cgacgctact caaaagtttc
     5041 gcctccttaa aaattaagtg cgtaattcca ttaaaaagag tgagcgaaaa atccgctcac
     5101 tcacagttag tatcattacc tataagaact ataccatact ctgcttaaaa ttacaagcag
     5161 acaggaaaac agatgcttat agcatatcga ataacgataa ttgattttca tctggtagat
     5221 ctttcaatac gccattgtcg ttcatatatt cgatcaatgt ttttgaaact ttaccgcggg
     5281 tggcaagatc ttctttggat aaaaatggac cgtcttttct tgcttcaacg atttgtttcg
     5341 ccacgttggt tcccaaactt gggacagctc taaatggtgc gatcaacgta tcaccttcaa
     5401 taacaaagtt aacggcatcc gatttataca aatcaatcat tccaaatttg aatcctcgtt
     5461 ccagcatttc attggctaac tccagcactg tcagttgatt cttttctttt acggaagcat
     5521 ctaatccctt gtccgtgatt tctttcattg cttgtttcac agcatctttt cctttgcaca
     5581 tggagactag atcaaagtcg tctgcacgta cagaaaagta tgcacagtaa tacaaaatag
     5641 ggaaataaac cttgaaataa gcgacacgaa gagccatcaa tacgtaagct gcagcatggg
     5701 ctttcggaaa catatatttg atttttgaac aagaatcgat ataccagtcg ggcacattat
     5761 tttctttcat cgccgataga taggtttctc gcaattcatc agggatcttg ttccattgtc
     5821 ctttacgaac agtttccatg attttaaatg ccatcccgct atctaatcca gcatggatca
     5881 aataaaccat aatatcatca cgacagccga ttacttccgc cagtgtcgca tctcctcgtc
     5941 gaatcaattc ttccgcattt cctaaccata cgtctgtacc atgagatagt ccggaaattt
     6001 gcagtagttc tgcaaaagtt gtaggatgcg tttcttctaa catccctcga acaaaacgag
     6061 tgccgaattc tggaatacct aatgtccctg ttttggaata aatttgttcc tgacttactc
     6121 caagtacttc aggaccggca aatatccgca tcacttccgg gtcatccgta gggatcgttt
     6181 gtgggtcgat ccctgataaa tcttgaagca tccgaataac agttgggtca tcatgcccta
     6241 gaatatctaa tttcaataca ttatcgtgaa tcgagtggaa atcaaaatga gtcgttttcc
     6301 attctgaatt ttgatcgtct gctggatatt ggatcggcgt aaaatcatag acatccatat
     6361 aatcgggaat aacgatgatc ccccctggat gctggccggt tgtccgtttg actccagtag
     6421 cgccttttgc taatctgtcg acttctgcac ttcgaaactg cagattattg tccctttcgt
     6481 atccttttac atagccatat gctgttttgt cggcgactgt accaatcgtt ccggcacggt
     6541 aaacatagtc ttcgccaaac aatactttcg tatagttatg ggcttctgct tgataatcac
     6601 ctgagaagtt caaatcaata tcgggcactt tgtctccatg gaatcctaaa aatgtctcaa
     6661 atggaatatc atgaccgtct ttgtttaagc gtgcaccaca ttttggacat tgtttttctg
     6721 gcatgtcaaa tccagaacca tacgtaccat cttcaaaaaa ctcggaatat tggcactctg
     6781 gacaataata atgaggggct aatggattaa cttcagtgat tcctgtcatc gtcgcaacaa
     6841 agctagagcc aacagagcca cgtgaaccta ctagatagcc atcttcatta cttttatgca
     6901 ctagtttttg ggcgatcaga taaataaccg agaagccatt accattaata gaattcaact
     6961 ctttttctaa tcgtttttga atgatctcag gtaacggatc tccgtacatt tgttttgctt
     7021 ttgtgtaact caattctgat atttcatctt ctgaacctgg aatcttcggt gtataaagtt
     7081 catctttgac tggaatcact tcttcgcaga tatctgcgat tttattcgta ttttctacta
     7141 cgatctcttt cgctgtttct tcccctaaaa agtgaaatgc agttagcatc tcatcggttg
     7201 ttcggaaatg gacatccggc aaactatgac gattcaacgg attcgctccg cccatagaat
     7261 tgatcaaaat ttttcggtaa atggcatctt cttcgttcag ataatgtaca ttacctgttg
     7321 caacaacgat tttatccaaa ctttttccga tttctaccaa attttggata atttcttcca
     7381 aatcatgttc attttttacc aattcttgct cgatcaaagg cgcatagacc gcttttggca
     7441 taacttcaat ataatcatag aattttgctc ggtttcttgc ctcttctacc cctttttgca
     7501 tcatggcttc gaatatttct cccttatcac aagctgagcc gatcaataga ttttcacgca
     7561 tctttttcaa ttgggagcgt ggtattcgtg ggacgcgttc gaagtattcg acattcgaca
     7621 ttgaaatcag tttgaacaaa tctttcagtc ctgcctgatt ttttgctaaa atcgttacat
     7681 ggaaaggtcg agcccttttg taagaatctc cttctccgat atgctcattt agctgatcat
     7741 gataaagcat gttatgattg tccattgcct ctttcacaaa gatccatgct aagtgaccag
     7801 ttgcttcagc atcatagatt gctcggtgat gctgttccaa gctaacacca aactttttag
     7861 ataacacacc taatccgaaa cgtttgaatt gaggatacag atatctagct aattccaacg
     7921 tatcaatgac tggattggcc gcttcaggaa taccatacct tgcgtagcta gtattcaaga
     7981 accccatatc aaaagccgcg ttgtgggcta ccaaaattgt atcttttgaa aattcaagga
     8041 acatccgcaa tacttcttct tccgactttg accctcgaac catttcatcc gtgataccag
     8101 tcagatctac cgttgttcgt gacaatggat gtcctggatc gataaattca tcaaagcttt
     8161 cgatcacatt tcctttatac attttgactg cggctagctc gatgatcgtg tcatatactg
     8221 cagacaatcc tgtcgtttcc acgtcaaata cgacgtatgt ggcttcactc aatgcttcat
     8281 gtgcatcatt ataagcaatt gggacaccat catctacgac attcgcttcc acgccgtata
     8341 agattttcac acctgctttt tttccagcac tatgtgcttc cggaaaggct tgcgcaccac
     8401 catggtctgt aatggcgata gctcggtgtc cccattttcc tgcttgtgcc acaagatcag
     8461 agatgctgtt tgttgcatcc atcgtactca tattgctatg aacatgaagc tctactcgtt
     8521 tttccccttc aggagcataa tctttgcgag gtgtatgttt gacttctatg atatcttgcg
     8581 cattcatgac caaatcacgg acaaacgtat cttcttgaat acttcctcgt acctttaacc
     8641 agctaccgac actgattgca tcaaatactt gttcatcttt ttcgccgttc gagaattttt
     8701 tgacgataaa tgatgaagta taatctgtga ttttcaaagt caagatcttg cgttttgaac
     8761 gtagttctct gacttctttg tcgaatacgt acccctcaag tgtgactctt cgttcttctt
     8821 caacgatact gatcattggt gtcgttggtt catcggctgg aatatttcgt cctagctgga
     8881 tgggtccttc aagagctggc gattgctctt ttctttcctt tttcttttgt tcgtggacaa
     8941 ctagactttc agctgcttgc ttcataaagg cttctgcctg ttcttgtttg cgttcttcaa
     9001 aaagtttcaa tacacgttcc gcttgctgct catccacttc tggttcgata cgaaattttg
     9061 ggaatccgta actgacgaac aactcttcta ctaatggcaa gtattgttgc ttcaaataag
     9121 aaatagctcc tgtactgtcg attggcaaga tgactttacg atcttttatc acaggtacct
     9181 gcgttttcaa tacttgctgt gctagtggtg tatcgcattg ctgattttcc aaagcttggg
     9241 cccagtaatc ttgcaagagc tgttcatcga atgtctggtc atctgctaag atattcagtt
     9301 tgacttgtgc aatatcttta aaagcgatag tcagatgctg agtcaatgac cgatatagca
     9361 taataggcaa aattttggaa aaatgtagcg taaattccca taaacgcgat ttacgatgga
     9421 ccactacact atccatttca ccagcagtaa gcagtggatg atttttttct gcttcctcta
     9481 gttggatttg ttccaacagt ttttcaaata aatctcgttt ttctgacaaa gatatacgcc
     9541 ctttctgatg agaaacaatg caattgatcg catcttatcc tcttcttttt cagtaccttt
     9601 tagtatactc gttttttctg agttttacat taaaaaattg gaactgcgat tcaaatcgca
     9661 gttccaattt taaaagcgaa tcatcagatt gtcaaaaaca gctgattcag ctatttttat
     9721 tttctgtgtt ttaacgctta ttattcgcct tggttcaaga ggattggtaa agtattcgcc
     9781 aattcttctt tacgtacttc aaccatttcg ccagttttct tgattttcac ttctacgatt
     9841 ccatccactg cttttttacc gacagtgatt ctgattggac agccaatcaa atcagaatcc
     9901 gcgaatttca ctcctgcgcg ttcatttctg tcatcaacca gtacttcata gccagctttt
     9961 gtcatcgctt cttctagttc attcgtcagg cttgtttggt attcgtcttt tagattcatt
    10021 tggacgatat gcagatcaaa tggcgcaatt ccttttggcc aattgattcc ttgttcatct
    10081 gaatgttgtt ctacgattgc tgataataaa cgacttacac cgataccata acagcccata
    10141 atcacaaatt tttctcgtcc attttcgtct agtacagttg ctcccataga ttcactgtaa
    10201 cgagtgccga ttttgaatat atgtccgatt tcgattcctc gagtaaaggc aagaacaccg
    10261 ttgttgtctg gtgaaggatc tccttcttgt acaaaacgaa gatcttcata agaaagcaca
    10321 gtgaaatcgc gttttggatt cacattttta tagtggaatc ctgtttcgtt cgctccagta
    10381 atggcattcg ctaaatcttg gacataacgg tctgcatata cttttacgtc atctgaaaca
    10441 ccgactggtc cgacagaacc aaactcagca cctagatatt tttgtgcttc ttcttctgtt
    10501 gcttcttcta aaaagtcagc agataaaaag tttttcagtt ttacgtcatt gacttcatgg
    10561 tctccacgta ccaaaacaag gacaggttgt tcatccgcaa tgaacaatac agacttgatg
    10621 attttttgag gttcaacaga aaagaaatct gctacttcgt caattgattt tacgttaggt
    10681 gtagctactt tctccaattc aagctgtgtt tcatgcgatt tttttgatac atagtagctt
    10741 gtagccatct ccaagtttgc agcataatca ctttcagtcg aataacagat cgtatcctcc
    10801 ccgatttctg agatcgccat gaattccttt gagtctttcc cgcccattgc gccgccgtct
    10861 ccgatgatcg agcgaaattc taagccacaa cgcttgaata cttcggtata ggctttttcg
    10921 taatctttat atgtctcgtc caaactttct tccgttgcat ggaaagaata tgcatctttc
    10981 atgataaact ctcttccgcg aagcaaaccg aagcgaggtc gtttttcgtc acgatattta
    11041 ggttggattt gataaagatt taaaggtaat ttcttatatg aattgatttc attacgcatc
    11101 aaatcagtaa aagtttcttc gtgagttggt ccaaggatca tttttcgatc attgcggtct
    11161 ttgaaacgat acaagtttgg tccatatgtt tcgtaacgcc cggattcttc ccaaagttct
    11221 gctggcaaga tggcaggcat cagcatttct accgctccga ttttttcaaa ttcttcccgc
    11281 ataatgcgtt tcagtttttc caatacacga tttgctaatg gcagatatga ataaataccc
    11341 gctgatactt ggcggatata tccagctctt agcaagattt gatggcttaa tacttctgca
    11401 tcatttggaa cttcgcgtaa tgttggaatc aacatttttg attgtctcat tctatatact
    11461 cctttatttc ctcaaaatag ttatcgtttg ttattatacc ctatttgtca actttttcta
    11521 gaaaaagaat cgttgaatat cgttccatgt aactagcacc atcaacaaca ttaaaaatcc
    11581 aaagccagct aatgtcaata ttccttcttt ttcttgactt aatggtttcc cgcgaatccc
    11641 ttcaaaaata ttcaagacta atttcccgcc atctaaggca ggaatcggaa gcagattgac
    11701 gattccgaga ttcattgaaa gaagcgccat caaactaata acagttgtta caccttgatt
    11761 tgctgcttct gaagacaatt ggtacatcat gacaggtccg cctaatttat ctaaactgaa
    11821 acctgtaaat agtgatccta aggctttgaa aatttccaat gaaccgctga aagcttgacg
    11881 cgtccctcca atgatcttat ccataaaacc tgtattcatt ggggctttta taccaagttg
    11941 gccaactttt tcgccatttg actctacaga tttcggtgtc acgtcgacag attgcacctg
    12001 accttctcgt tcgatcttaa agtctaatgt tttacctgga tttttcgtaa tgactgttgt
    12061 cagatcattc catgaatgga tttcttttcc gtccacactg actacctcat cattttcttt
    12121 taagcctgct tctgccgcag ctccattagg catgatctct cctacgcgat tagtgttcgt
    12181 cacttgaaca ccaccttgca taaatgccag aatgatgaat aacacgattg ccaaaataaa
    12241 gttattcatg ggtccagcaa agttggtcag catacgctgc cacaatttag ccgattggaa
    12301 ctgtacgtct tttggcgcaa ttctgatttc tgttccgtcg ttttcaataa tcgtcgcatc
    12361 gtgatcaaca gggtaggtta ctacttctgt ttcgtctcca ttcacataac cagtgatagt
    12421 cagctcatct tctaaatcat aacggctcaa ttccatcggg atagcatttg tcaattggat
    12481 tttcttactt aaatttatct tctctacgat gccgtcagaa tttagtaaaa gagaaagagg
    12541 cattcctggt gccatttctg tttcatcatc cccattgcct gccattcgaa cataaccgcc
    12601 aataggtaat aggcgaagcg tatatgttgt cccgtctttt gcttgatgcc cataaatttt
    12661 cggacccata ccgatagcaa attcgcggac gaggattcct gatcgtttcg caaagaagaa
    12721 atgaccaaac tcatgaacaa tcactaatat accaaaaacg atgataaatg tcagaatcgt
    12781 tttcatagct tttttctcct ttacttgttt tttgtgtttt ttataaaaca acgataaaac
    12841 actataattt taccatagta taccaaagac accaatagta tcaatataat gaaaaataaa
    12901 aacctgcgtc ttttatgaaa ttcacaaaga aacgcaggta tacttactaa ataatgccaa
    12961 ataaatgcat gataggaaat acaaacagca aactgtcgaa acggtctaag attccaccat
    13021 ggcctggtaa gatatttccc gagtctttca catcataatg acgtttgatt gcagattcga
    13081 ccaaatcacc aaattgtccg actgctgaaa aaataatcgt catcagaagc atgacaaaca
    13141 taccatgatt gaatagttct tttgctgggt atagcaataa ataaagaaat gcgactacaa
    13201 cagcactacc gataccaccg agcgcacctt caatcgtttt gttgggcgaa atatcaggac
    13261 ttaacttatg ctgaccataa cgtcgaccaa ccatatatgc tccgatatca gttgcccaaa
    13321 caataaatag accaaatatc aaaacagcca agctttctgt tcgtgcattg acaaaattct
    13381 ggaaacctat tcccacatac aagctgacaa gcacaggaaa acctgcttca tcaatcgtat
    13441 acgtattttt agaaataact gatgttccta aaagaatcat aactataaga taaaataaca
    13501 taaatgtact tacattctct ggaagaaaga aaaaccatct ttcttttggc aaaacaagta
    13561 acactgcacc aacggcggat aaaactcctt caaaacttaa gagtgtcaat cctttcatcc
    13621 ggaacagctc gtaaactcct actaccgcta agattgctgc tgtaagctca accgctatgc
    13681 ctccgatcca tataatcgga ataaacaaga ctagcgccac agctgctgtg atcacacgtt
    13741 gtttcatgac tgatcccctt tctcatcttc tgtttttttt acaccgccga atcgacggtc
    13801 tcgattttga taagaggcga ttgcttcttc aagatgcgca ccatcaaaat caggccaaag
    13861 cgcttttgta aaatacagct cactatatgc gatctgccat aacagaaaat tactaattcg
    13921 ctcttctcca cttgtacgga tcatcagctc aggatcttgt aactctttag gcaagaaacc
    13981 tgtcattaaa tgcttagcaa tcaattcatc atcgatttct tctgcgtgta tttctccttt
    14041 tgctacttct tcggcaattt cttggacagc agtcacgatc tctgctctac ttccataatt
    14101 caaagcaaaa ttcaatacca ttccagtatt gttttttgtt tgctcaattg cacgtcttac
    14161 agcatcttgc gtatgttctg gcaatacgtt ttcataaccc atgacatgaa ctttgacatt
    14221 ttctttaatc aattctggta cgaacgtatc aaagaagtcg acaggtagtt gcatgagaaa
    14281 acttacttca tcttttggtc gcttccagtt ttcagtcgaa aaggcataaa gcgtcaacac
    14341 tttcactcca agccttgatg ctttttttgt gactttcttt actgtttcca tgccttcttt
    14401 atggccagca actcgtggca aacgacggtt ttgcgcccaa cgaccattgc cgtccatgat
    14461 gatggcaatg tgctgaggaa ttggtccatc ggtattaaat tgaaattcgc ttttttcttg
    14521 aatatatttg tttttttgcg gaaaaaaacg taacattctt tttcctccta tacttaaaag
    14581 attcccacta tcttaagtat tatagcaata tctaactgtt tttcctatga aatcattgtt
    14641 tttttattta aaaactgaat aattttttct ttttttaatt tttaaataaa agaaatataa
    14701 ataaaaacag aggaagcgga atgatccgcc cctctgtttt tatgatttta ttaaacttcc
    14761 aataattctt tttctttatc agcagcgatc ttatctactt ctttaatgct gttatctgtc
    14821 aatgtttgta cgtctttttc taatccgcgc aaatcatctt cggtaatatc accatttttt
    14881 tgttgttttt tatattcatc gatcgcatca cgacggatgt tacgaatcgc gatttttgca
    14941 ttttctgctt cttttttcac atctttagct aattcttttc tgcgttcttc tgtcagttgc
    15001 gggataacta agcgaatgac atttccatca ttggttggac tgattccgat atcacttgcc
    15061 ataatagctt tttcaacatc ttgaagaatg ctcttgtcaa aaggtgtgat cattaaaaca
    15121 cgtgcttctg gaatttggat tgatgccatt tggttcaatg gtgttggcgc accgtagtag
    15181 ttcacagtaa tacggtccaa aaggctagcg ttcgcacggc ctgcgcggat ttgcccaagt
    15241 tcacgttgaa gattttgtgc tgcttttttc attttttctt ttgcttctgt cataattgca
    15301 tctgccattt ttatttcccc cttacagttg ttccgatatt ttcgcctaag attgcacggc
    15361 gaatatttcc gtgttcattt aaattgaaga caagcagtgg aatatcatta tccatgctta
    15421 gtgaacttgc agttgaatcc atgacttgta atcctttggc aataacttcc agatgagtca
    15481 gttcttcaaa ttttactgca tttgcatcta ctttcggatc agcagaataa acaccgtcta
    15541 cattgttttt agccatcagg ataacatctg cgccgatttc tgccgctcgt aatgctgctg
    15601 ttgtgtctgt cgagaagtac gggttgccgg taccgcctgc aaagataacg atccgtcctt
    15661 tttctaaatg acgttctgct ttacggcgaa tatatggctc tgcgatttga cgcatttcaa
    15721 tcgaagtttg cacgcgtgtc ggtacaccca cgttttccaa cgtatcctgt aaagctaagg
    15781 cattcataac tgtagctaac atacccatgt aatcagcttg agcacgttcc atgcccatat
    15841 gtgctccgat ttgtccgcgc cagatatttc cgccacccac aacgattgcc atttcaacgc
    15901 ccagctcgtg aacttctttg atttcttgga tgatttcttt gattactggt ggtttgattc
    15961 caaatcctgc atccccagct aaagcttctc cactcaattt taagactaca cgctgatatt
    16021 tcggtgtgac catgttcatt cctccaatat tttctattgc cattttacca tacttagatg
    16081 atttcgacac tctaagactg gtttttcttg ttttgcttaa aaaaagggag cgcacaaacc
    16141 gtgcgttccc gtctgttatt caacagaatt attttttcac ttggctcata acttcttcta
    16201 caaagttatc ttcgcgtttt tcgatacctt cgccaacttc gaaacgtacg aatgttttaa
    16261 cagttgcgcc ttttgatgca acgtattttt caacagtcat atcaggatct ttaacgaatg
    16321 gttggtccac caaagcgatt tctgctttga atttcttcaa gcgtccttct accatttttt
    16381 caacgatatt cgctggtttg ccttcgttca atgcttgttc tgtaagaaca gttttttcat
    16441 gttctaattc agcttctgga atttgtgatt cgtttacata acgaggattg atagctgcta
    16501 cgtgcatagc tacgtctctt gcaactgttt cgtcagttgt tccatctaga acagtcaata
    16561 cggcaatacg tccgcccata tgcaagtatc caccaaatgc agcattgtcg tctttttcta
    16621 caacttcaaa gcgacggaag ctgatttttt ctccgataac ttgagttgct tcgatcaagt
    16681 cagattcaat tgtaccttta tctgttttga ttttcattgc agcttccatg tctgctggtt
    16741 tgttttcagc aacaagttct gcaatttctt ttactaaatc ttggaacatt tcgtttttag
    16801 aaacaaagtc tgtttctgag tttacttcaa cgattgctgc agtgttacct ttcacagcaa
    16861 ctgaagccaa tccttcagct gcaatgcggt catttttctt cgcagccttt gccattcctt
    16921 tttcacgtaa aagatcaact gctttttcca tgtcgccttc tacttcaact aatgcttttt
    16981 tcgcatccat cattcctacg ccagtcattt cgcgtagttc ttttaccatt ttagctgtaa
    17041 cgtctgccat gtttaactcc tcctgtgtgt ttttcttttt aaaaaaagct gcttcaaagg
    17101 agagtgaggc ttgtcgccca gcctttgaaa cagctccatc attcaaaatt attctgctga
    17161 agaattgtcg ccttcaacaa catctacgat ttcttcgatt gatgttgctg tgttttcttc
    17221 tgtgaaatct tcttcaacta cttgatcttc accttggttt ccttcgatga aagcatcagc
    17281 catttttgaa gtaatcaatt taacggcacg aattgcatcg tcgtttgaag ggattactac
    17341 gtcgatttca tctggatcgc agtttgtatc aaccatagct acgattggga tatttaattt
    17401 gtgtgcttct tgaacagcaa tacgttcttt acgtgggtca acgatgtaca ttacatctgg
    17461 gattcttggc atatcagcga taccgcctaa gaatttttca agacgttcac gttctttgtt
    17521 caaaccagca acttcttttt taggtagaac ttcaaaagtt ccatcttctt ccattgcatt
    17581 gatttgtttc aaacggctga tacgtttttg gatagtatcc cagtttgtca atgttccgcc
    17641 taaccaacga tggttaacga agtattgtcc tgcacgagtt gcttcttctt tgattgcttc
    17701 ttgtgcttgt ttttttgtac ctacgaataa tgctacgccg ccttcttcag caacgctctt
    17761 catgtaatca taagctgcat ctactaattt aactgttttt tgcaagtcaa tgatgtagat
    17821 tccgtttctt tctgtgaaga tatatttctt catttttgga ttccagcgac gagtttggtg
    17881 accaaagtgt acgccggctt ctagtaattg tttcatagaa attactgcca tgtttgtttc
    17941 ctccgatttg gttttatttt tcctcttctt atcttctacc ttgccggcaa actttttcaa
    18001 gcacctaccg acaatccaat aagaatgtgg atttgctgat gcataaagca ccctcagcta
    18061 ttatacagac aaaagatccg tatcgcaaga gcaaatatac ttttctgctc aacattcaat
    18121 agtttacctg aaagataaaa agaattcaag aaaaacagta gatttctctt tgtttcttta
    18181 ttatcctttt tccataaaat aattttaggt ataaaagacg aatattattg ggcaatcctt
    18241 cttttttttg ttatgataca tgtgaaatct ttcgtaaagg agaaagaaaa tgcgcaaagc
    18301 tcaaagacat ttgctgatta aacaagtcat tgaagaattc ccgatccgta cgcaagaaga
    18361 actattaaac aaacttgggc attatggcgt taccgccacg caggcaacga tttctcgaga
    18421 tatccgtgat ctaaaaatcg tgaaggcacc tgatgaaaat ggcgtttccc gcttcgtatt
    18481 gtttcgcgaa aaaggaactc ctgagactag gactgaggaa gaggatcggc tggttcaaat
    18541 gattgaagac attgtattga aagtcgaacg agtccacttt ttgacgatcg tcaatacctt
    18601 gccagacaat gctcatttat ttgcatctgt tttagatgag atcaaacctc cctctattgt
    18661 cagtacaatt gctggattcg atacgatcat ggtcatctcg gaaaatgagg aggacgcaaa
    18721 acgagtagaa gaatttcttc accatccaac tgaagtatca cttttttaat cagagcacgt
    18781 attcctaata tggaatgcgt gtttttttat tttcaatatt ttccttatta aataaacctt
    18841 tacactcttt acaaaaatac atcatttttt attcaatttt ttcaacacct tgattgaatc
    18901 acaataatca tcatttatat tggatgttaa caagagcagc ttcttgctct ttgtgtattt
    18961 caaaaaagaa atgaaaggaa atcatatgta taaattatta actcctggac cattgacgac
    19021 ttctgaaaca gtgaaaaatg ctatgctgat cgatcattgt acttgggatc aagaatacaa
    19081 agaaattaca caatggataa gaaaaaaatt attggatctt gctcaagtat cggaaggaat
    19141 atacacaacg gtcttaatgc aaggtagcgg ctcctttggg gtagaatcgg tattaagttc
    19201 tgtgatcagg ccagaagata ctgtactgat ttgttcaaac ggcgcatatg gacaacgaat
    19261 gatcaacatg tgcgaatgtc tgaaactgaa ctataccgtt tatgaggtat cggaacatca
    19321 aatcctccaa gctcatgtga ttacacagct gattgaagag aatccctcta tctccgcact
    19381 agcaatggtc catagcgaga ctacttccgg tattttgaat aatttggaag agattgctgc
    19441 tgtcacacaa gaaaaagatc ttttgtttat agtggacgcg atgtcaagct ttggtgggat
    19501 accgattcgt gtagccgaac tagggattga ttttttgatt tccagctcaa acaaatgtat
    19561 tcaaggtgtt ccaggctttt cttttgtact tgctaataaa gcggaactag aaaagcgaaa
    19621 aaatgtgagt cgaagtttgt cacttgatct atatgaccag taccgcgtga tggaaaacga
    19681 gaatggcaaa tggcgtttca cttcccctac ccatacggtt ttggcttttc aacaagcgct
    19741 gaaggaacta gaggcagaag gaggaatcga aactcgatat tcacgttatc tcgccaataa
    19801 tcatgtacta cgacaaaaaa tgacagagat ggggttccag cctttcattc aagaatatca
    19861 ggggccattc atcacctctt ttctttaccc taaggactgg cgatttgatt ttaatcattt
    19921 ttatcagtat ttaaagcaaa acggatttgt gatctatccc gggaaaatca gcgatcatga
    19981 atgttttcgt atcggaaata ttggcgagat ctatccggaa gatattttgg aactgacagc
    20041 aattatcaaa gactataagg agaacgttaa tgattaaaac tgtaattttt gattgggcag
    20101 gaacaactgt ggactttggc tgtatggctc ctgtccacgc ttttagaaat gcttttctag
    20161 aaaaaggaat ccaactgact gacaaagaaa tccgcgaacc gatgggaaaa ttaaaatggg
    20221 atcatatcca acagcttctt tccctccctt ctgtcaaaaa acaatggata gctatttatg
    20281 gacaactacc tcaggaaaaa gaggtggagg aattgtatca acaatttgaa cattatctgt
    20341 ttaaagattt ggtcaagcat tctaccttga aacccgacac attggaaacg atcgaacggt
    20401 tgagaaacgc tgggatcaat attggttcca cgactggatt tactgctcct atgatgacaa
    20461 tcgtagcaga aaccgcaaaa aaagaagggt attcaccaga cttcatggca acttctgaag
    20521 acgtcgaagg gtatggtcga ccgtttcctt atatgatcca taaaaacatg cagtacttca
    20581 aaaacaagtc ggttgatgaa gtaatcaaag tcggagatac ggtctcagat atccaggaag
    20641 ggaaaaatgc cggagtgttg acagtaggtg taattgaagg tagttcgcta atggggattt
    20701 cttatgatga gtaccaaaaa ttagagatgg ggaaaaaaga tcgtttaaaa cagtctgttg
    20761 ctcttagatt ccaacaagct ggcgctgata tttgtgtaaa caatttaaaa gagttaacag
    20821 aatacatttt gtacgctgac aagaatccag aatgactttt atacaaaatc agaggtcgtg
    20881 aaaaagctgt actgcatcaa gtaataagaa cagccaaaca aaaatagctc ctcatatttt
    20941 tcgtttggtt aggcttatta ccgcagatgc aagcttttga acaccgttta ttcgaaaaaa
    21001 aaagggagct gtgacacgac ttttgtcaca gtccccttat ttacaaacaa acagtgttta
    21061 agagacaacc tctccactaa acggtgttca ttcagctgac tctcggtatt tgctcaaaaa
    21121 acgataaaat ttgaaaagct attttctctt ttgtttctct aatactcgga tcagaacgct
    21181 gatttcacat tctctttttc ttacatctta tctggtgcat gaagtcctag caaacgaagg
    21241 tcttctttca ataatacagt cactgcataa actagagcta agcgtgcttc tttttgttca
    21301 tcatcagcta aaatcttcgt atgagcatag tatttgttga atgcttgtgc taatttgatt
    21361 gcatgtttcg caatcactga cggttcgtat ttttccccag cagacaagac cgtttcaggg
    21421 tatttttgaa ctaatttaac cacttcccag ctggtatcgt cattcaatgc atagtctgct
    21481 gtttctgaag ggacaaatcc tgctttttcc aacaggctga ctgcacgagc atgtgtatat
    21541 tgcacataag gtccagtttc tccttcaaat cgaacaactt cttccaggtt aaagtcaaat
    21601 gtattcagtc gatcgttttt caaatcgtgg aaaatgactg ccccaacccc aacttgttta
    21661 gctacagcat ctttgttttc caaatcaggg tttttctcgc tgatttgttc tttagcagat
    21721 tggattgctt catttaatac ttcttctaat aagacgatct ttcctttacg agttgataat
    21781 tttttaccac cttgcgtgat caaaccaaat ggaatatggc gcatatcgtc agaccagtca
    21841 aagcccattt ccttcaagac agctttcaac tgtttaaaat gatagctttg ttcattccct
    21901 actacatata gagattgttt gaagtcgtaa gtacgtttac ggtacaatgc agcagctaag
    21961 tcacgtgtaa tgtatagtgt cgcaccgtct gacttcttaa tcaacgctgg attcaggtca
    22021 taagcagata ggtcaacgat ctctgcacct ttatcctcgt tcagcaaatg tttttcttct
    22081 aaaagtttga cgatttcatc catcttgtca ttgtagaaag cttcaccgtt caacgaatcg
    22141 aaacgtactt ccagcagatc atagatttta ttgaattctt tcatcgattc atcacggaac
    22201 cattgccata attgaatagc ctcttcatcg ccttcttcca gacgtttgaa ccaagcacga
    22261 gcttcttcat ttaattctgg ttctgtttca gctacttcat ggaattggac atataaacga
    22321 agcaattcat tgattggttc tgctttaaca gcttcttctg taccccattt tttgtaagcg
    22381 acgatcagtt ttccaaactg tgttccccag tcacctaaat ggttgatgcg gattggctga
    22441 taaccgattt tttccataat gaaaccgatc gaattaccga taactgtcga acgcaaatgt
    22501 cccattgaga ttggtttggc aatattagga gaagacatgt cgattggtac tgttccttga
    22561 ttcccgatat tgctatcccc ataatgttct ttttctttca ctactgtttg caaaactttt
    22621 ttgctgataa gttctttatt catgaaaaag ttcaagtaag gtccgacaac ttcgattttt
    22681 tcaaagttgg cagaatcgat tttttctgct aaatctgcag cgatttgctg tggcgctttt
    22741 cggtaaactt ttgctaatga aaaggctgga aatgcaacat ctccgtgttc agctgatttt
    22801 ggattttcta gtaattgttc gacttgttcc agtgttaaat catctttgac agcgtcatag
    22861 acagcttttg cgacaagatc tttattattc atctttattc ctcctaaagt gctcccttga
    22921 ttgttaaaca aaaaaaatct ctagcgacgg catacttagt atgccatcac tagagacgag
    22981 atttcccgcg gtaccactcc aattgtccta aaaaaggacc agctccatct gatatcggta
    23041 gacgaccgtc tgtttaaaac agaattctcc aagtgcgctt cgttgtatct tcttcattcg
    23101 gcttccacca cttccgaatt cgctagccaa gaatgataca agtactctct tgttcaacaa
    23161 atcttagtct tattaaactt tcttgaatta tatcagaaaa aaaaacgaaa ggctagtcgt
    23221 ttctgataaa aaaacaatcc agatggccta aaggagaaga aataccacct ggatagctat
    23281 aaatcatttc gtaaatacga aagacttaac aaattaatca gctacgataa tcgttccttc
    23341 ttcagaagct aaaagattct cgatattttc taaagaagtg atgattgcct tagcgtttgg
    23401 ttttgcttca acaaatgcaa tagctgcctc tactttcgga agcatgcttc ctggagcaaa
    23461 ttgcttttca tcaatgtatt gtttcatttc tgaaacagta acagtttcta atttcttttg
    23521 atctggtttt tggtaattga catacacatt gtctactcct gtcaatacga tcagcaaatc
    23581 cgcatcgatg atttcggcta atttttctga agcaaaatct ttatcgatta cggcttctcg
    23641 tccttctaat ccagtagctg tttctacaac aggaatccca ccgccaccaa cagagactgt
    23701 gataactcct tgatcgacca gcgtttcgat gacacgtgct tctttaatcg agatgggttt
    23761 aggggaagcg actacttttc tccatccacg tccagcatct tctttgaatg tcacagtgga
    23821 gtcagcattc atttgttctt tggcttcttc ttccgtgtag aatggaccga ctggtttact
    23881 tggatttttg aatgaaggat cattttcatc cacaataact tgagtgacta aagaaaccac
    23941 atctttatcg atcccttttt ccttcagtac ttcgcccatg gcattttgta accagtaacc
    24001 gatagatcct tctgtcattg caacacatgt atctaaaggc attgccggtg ttttctcaga
    24061 atcagcagct tgctgctgaa tcaataggtt accgacttgc ggaccatttc cgtgggagat
    24121 gatcaattca tctccttgtt cgataaattt cactaaatac tttgctgttt ccattaacgc
    24181 ttcttgttgg gcttttgcac ttgcatccgt agacaagatt gcattacccc ctaaagcgac
    24241 aactactttt cgtttggcca tttcattcgt cctttcttca gttctaccga attttctaga
    24301 taacaaaacg agccagcgtt ttgtgatttt ccctcaatgc tgactcgttt tttcgtgaga
    24361 tgaatgtggt tatgcttaaa cgcgagggat aaacaagtta cctaatgttg ctgccatgat
    24421 cgctttgatt gagtgcatac gattttctgc ttggtcaaat tgacgagcat atttgctgcg
    24481 gaagacttca tctgttactt ccatttcagt gatgccaaag cgttctttca tttgttcacc
    24541 gtaaacagtg tttgtatcat ggaaagctgg caagcagtgc aagaagatca aacgatctgt
    24601 gttgtgtgtt ttttctacca tttccatatt gatttggtaa ggtttcaaca atttgatacg
    24661 ttcttcgaat ttatcttctt cacccattga tacccataca tcagaataaa ggacgtctgc
    24721 tccgtctaca cctttatcaa catcgtcggt aatcatcaat tgtgcaccgg atttttcagc
    24781 aaagccttct gccattttga cgatttcttc ttcaggttgt aattcttttg gtgcaacgat
    24841 acgcatattc gctcctaaaa ttgcacctgt gactaataaa gagttagcca tattgtttcg
    24901 accatctccg cagtaagcaa cagtgatacc ttctactgtt ccaaagtttt cttggatagt
    24961 taaaaagtca gcgatcattt gtgttggatg ccattcatca gtcaagccat tccaaactgg
    25021 aacaccagag aattctgcta gttcctctac cattttttga ctgaatccac ggaattcgat
    25081 tccatcaaac atacgaccta gtactttagc tgtatcttct gtggattctt ttttacctaa
    25141 ttggatatca tttgcaccta gatattcagg atgtgcacct agatcgatcg ctgcagtagt
    25201 aaatgcagaa cgagtacgtg tagatgtttt ttcaaataaa agcgcaatat tttttccttc
    25261 tagatagtga tgtgggatac cacgtttttt caagtctttc aagtgttctg aaaaatcgat
    25321 caaatattgt aattcttctt ttgtaaagtc tttttctgct aataagcttc ttccttggaa
    25381 tactgattct ttcatattaa acattctcct ttagattgtt tttatttaag attaaagagg
    25441 aaatcccgac ggcagctgac agtaagaccg ccgtcaggaa acaaattact tcaagtcttc
    25501 acggacaagt ggttggctca tgcagcgtgg accaccacgg cctcgtgaca attcacttga
    25561 attgatttct aacactttga tgccatagct acgaagcaat tcgttcgata cataatttcg
    25621 gttataagtg actacaacac ctggagcgat tgccaatgtg tttgatccat cattccattg
    25681 ttctcgaggt gcaacgatct catctccatt accagttggg atcagtacaa gatcatctaa
    25741 tccaagagca gctttcatcg tttcgtgtaa gttatctgaa tgagtaatct tgatatcatc
    25801 accatctggt tcgattgtaa agacatccat cttaccgtct ttactttgaa ttgctggatg
    25861 gatagtgaat ttatcgtgat ctaccattgt aaatacggta tccaaatgca tcattgcacg
    25921 gttgtttgga attttgattg ctaacacttt ttcaaatcct gaattttttg cgaataggtg
    25981 gcgtgctaat ttttctaaag ctttcgcatt agttcgttga gaaatcccaa cagctactac
    26041 tttatcgctt aagatcaatt cgtctccgcc ttcgatatga tctggatttt cacgatccaa
    26101 ccatacttcg attcctttat ttgcaaatct tggatgatgt ttcaaaatat attctgtaaa
    26161 catcgattca cgacgacgtg cttcaaaagt catcttattg actgtcatcc catttcccat
    26221 tgatgcggaa gggtcgcgtg taaagtacaa gtttggcatt gggtccatgt aaaatggata
    26281 gtcatcgtct gctgacagat cgtatagact gctagaacgt acttcaatat cttttgtacg
    26341 aaccccagcc atgattttat ctaccatttc ttgcgtttcc attgatagaa ggaactcatg
    26401 aagaccctct cttacagcat tcgatgcaat atgagattca tccaacattt tattcaagaa
    26461 ttgttctttg acattgcctg cgtcaatcgc ttctgccgct aatttttcca agtaaagagt
    26521 ctcgacgcct tcattttgca atgtctttgc aaagttgtca tgttcttctt gagcaatcgg
    26581 aagatatgga atatcatcga acaacagacg atccataata tctggtgtca aattttccac
    26641 ttcttgtcca ggccttttca gtaaaactgt ctttaatttc cctatttcag agaaaacgtg
    26701 aataggttta tccatgttgt gttgcctcct tctttttgat tacatgtata ggatatcctc
    26761 cttttgtaat cactttcata attttgtctc attcaaaatg agacattcct cacattaata
    26821 ttcgataatt aaataattaa acgtttttct atttttaagc g
//
LOCUS       DACDPS010000031        26269 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.031, whole genome shotgun sequence.
ACCESSION   DACDPS010000031 DACDPS010000000
VERSION     DACDPS010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.031"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(171..973)
                     /locus_tag="IWJ07_001414"
                     /pseudo
     CDS             complement(171..973)
                     /locus_tag="IWJ07_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1194..2408
                     /locus_tag="IWJ07_001415"
     CDS             1194..2408
                     /locus_tag="IWJ07_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9333002.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            2430..4445
                     /locus_tag="IWJ07_001416"
     CDS             2430..4445
                     /locus_tag="IWJ07_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAP9333003.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(4528..5901)
                     /locus_tag="IWJ07_001417"
     CDS             complement(4528..5901)
                     /locus_tag="IWJ07_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP9333004.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(5927..6211)
                     /locus_tag="IWJ07_001418"
     CDS             complement(5927..6211)
                     /locus_tag="IWJ07_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9333005.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(6213..8228)
                     /locus_tag="IWJ07_001419"
     CDS             complement(6213..8228)
                     /locus_tag="IWJ07_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9333006.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(8407..8712)
                     /locus_tag="IWJ07_001420"
     CDS             complement(8407..8712)
                     /locus_tag="IWJ07_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAP9333007.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(8801..9832)
                     /locus_tag="IWJ07_001421"
     CDS             complement(8801..9832)
                     /locus_tag="IWJ07_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAP9333008.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            10039..10914
                     /locus_tag="IWJ07_001422"
     CDS             10039..10914
                     /locus_tag="IWJ07_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9333009.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(11066..11908)
                     /locus_tag="IWJ07_001423"
     CDS             complement(11066..11908)
                     /locus_tag="IWJ07_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9333010.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(12000..13877)
                     /locus_tag="IWJ07_001424"
     CDS             complement(12000..13877)
                     /locus_tag="IWJ07_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP9333011.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(13874..15295)
                     /locus_tag="IWJ07_001425"
     CDS             complement(13874..15295)
                     /locus_tag="IWJ07_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP9333012.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEELRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(15298..18687)
                     /locus_tag="IWJ07_001426"
     CDS             complement(15298..18687)
                     /locus_tag="IWJ07_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP9333013.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            18944..20335
                     /locus_tag="IWJ07_001427"
     CDS             18944..20335
                     /locus_tag="IWJ07_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9333014.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(20408..21055)
                     /locus_tag="IWJ07_001428"
     CDS             complement(20408..21055)
                     /locus_tag="IWJ07_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9333015.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(21063..21749)
                     /locus_tag="IWJ07_001429"
     CDS             complement(21063..21749)
                     /locus_tag="IWJ07_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9333016.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(21755..22828)
                     /locus_tag="IWJ07_001430"
     CDS             complement(21755..22828)
                     /locus_tag="IWJ07_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP9333017.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            23050..23724
                     /locus_tag="IWJ07_001431"
     CDS             23050..23724
                     /locus_tag="IWJ07_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9333018.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            23729..25069
                     /locus_tag="IWJ07_001432"
     CDS             23729..25069
                     /locus_tag="IWJ07_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9333019.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(25218..26204)
                     /locus_tag="IWJ07_001433"
     CDS             complement(25218..26204)
                     /locus_tag="IWJ07_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAP9333020.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         7655 a         4947 c         4818 g         8849 t
ORIGIN      
        1 aatacgcata acagataaat gacaaatgtt tacacctata tcccctgata atacaaccaa
       61 aagtttagaa ctgaaaataa atccaaaaaa attcgctgca acgatataat aagacagcaa
      121 tcgcacctaa aacaattttc cttcatatgc tttagtgaaa gattttcttc ttaatgttta
      181 tttctccatt cattgggagt acatgttgaa tacttcttga ataatttgaa taaagaagtc
      241 gtttgtttat atccgatacg attagcgatt tcttccaacg aaaaactcgt ttcttgaatc
      301 agttccaacg caaagtcgaa ttttttcttt agaaccagtt cttgaaagga agcatcaaaa
      361 tacattttca atttattact aagatagacc ggattgtagc caaaatgagc tgctgtttct
      421 tttaacgtac aagaagtaaa atgcttatcg atataatcta atactctaag catctcattt
      481 tctgcgcatc catctgctga tgaaaagctt tgttgttcta ataaatgagt caattcgatc
      541 agtaatagtg atgtatagag atttaacgaa cggatcttat gactgtatgt atcaaaataa
      601 atcaacagca tattttcaat caaatgcgtc aaacgacttg tttttccgcc gcgaaaatga
      661 atgaattgtg tatgttcccc tttttctttt gatgcattta tcaaaaaaga agaaagtaga
      721 ttatttgtgc ttgccaaatg attcaatatc tctgtagata aggtttcttc ttttaataag
      781 atgttgatta aaatatcctc ttgccctgtg tattcgattc tttgaggtgt ttactgatat
      841 gaatatttcc ctgttgaaat agatcccctc ggtggatttt tggcggtgtc tgaagatggt
      901 aagctaagtt taaatcattg atattcattc cttgttcacg ctgcagagtt tcgattgatt
      961 cttcgttacg caaaaaaaat gtctaattca gctagttcca attgactccc tcctttttgt
     1021 aagcggtttc tttcgcttat tagttcacgc tgtactgcta ttatatgcaa agctattttt
     1081 ggtttcataa agactttcta tggttgggac atgaacttcc tgtgattccg ctattgagag
     1141 aataaccact tcatactata tttttgataa gcgcttacaa aaatgaaagg aacatgctta
     1201 tgaataaaaa gatgatgaaa ttttctctgc tgtcagtctc tcttattttg acttctgctg
     1261 gtgcagtcac gtctgcaatt cccagtatgt cagctactta tgccacgcgt tctttatctg
     1321 ctgttgaatt attaacgact gctccctctt ttatggtcac tatttttgtt ttgattagta
     1381 gttttattgc taggaaaatc ggacaaaaac aaactgtgat catcggatta gtgattgcat
     1441 cgatttgtgg tatcctcccc ctcttctcac aaagttacag tattgtctta gcttcaagag
     1501 ctggactagg tattggcttt ggattgatca attctttggc agtcagcatg attgctgcct
     1561 tttatcacgg agatgaacga gcctctatga ttggttttca aagtgcattt caaggatttg
     1621 gtgcagcttt aatgacattt gttgcaggac agctagtaca gttcggttgg caatatacct
     1681 ttttagtcta cgccatcagc ttacctattc ttgtcttgtt tgttttgttt gttcctacac
     1741 ctgaaacaac tgaacttcaa tcgcatggca cagccccaac aagacaagta atcaaaacga
     1801 atcctaaaat gattagttat ggtatttttc tcttgctggt catcgtggta tataatgctg
     1861 tatcgattaa attagccaca gtcctgacga cttattctct aggaaacgaa actaatgcag
     1921 cgaatatctt aacaatcatg caattggcaa gtatggctgc cggagtttgt tttggctggc
     1981 ttcagacaaa agcacatcgc tatatgctta cactttcttt actactgatg ggaatcggct
     2041 ttgtattgat tgccactgtg ataaacctgt atgtgattgg gtttggtgcg atcctaacag
     2101 gaatttcttt ttcgttattc attccttact tattcaacca agtaagtatc caagcgccca
     2161 agtacgcgga gagtttcaat acttcaatct tattggttgg cgctaattta ggtactttca
     2221 ttgcaccata tggtttgctc tttctctctt ttgcttctct tgggagtaaa acacttgctg
     2281 ttttcgtcaa tggcagtatc ttgctgttga ttagcgcgct tattagcctt ctagtagtac
     2341 acaggacgaa tagaaagaca gtgataagag tagatgaaga aataaaagaa caattgacaa
     2401 caaaataaat ttgatggagg tcgtgtaaaa tgacaaatga aaaacataaa gatagatggc
     2461 tttggtatcc tggagacttt gagatccgcc atggactgct acaaaatttc caacgggaag
     2521 aacgaggttt tgactggcca gcgtactggt acatggatga ttgccatcgg aacgtcaagt
     2581 ttaagagata ttactttctt gatcaacctt ccatgtttaa agtgacgatc caaggagttg
     2641 ggtatgtaga aattaatgga caaaaacatc catgtggaaa atggctgact tgtccagctg
     2701 gtaaagcaaa aattcgtatc tttgtaggac atacatcagg gctaccagcg atgtttatcg
     2761 agggagacga agtaaaatca gatattggct ggacagcaag taattttatt gaagaatatc
     2821 cagctggctg gagtccgctt tatgtcgata tcgcaaaaga tccaaatcaa atctattatc
     2881 aaaaagaatg cgtccatcca gtcactgaaa cggagattgg cggaggaatc ttattcgatt
     2941 ttgggcgagc agtgaatggc tgtgtagaac taatatctct ggatgaccat atcgaatcta
     3001 tcactctatg ttatggagag tccgatgtag aagctatgga tatcgattat tgctactata
     3061 agcaaatagg aatgacaaga aaaatgaaag ctcgtaaacg cgcttttcgc tatttgttta
     3121 taccagaggt ttctactgga atggttcaac ttcaagtttt tcatgaattt ttatctcttt
     3181 cgaaacaagc tgctttttca agcgattctt ctttactgaa tcagatctgg tcagtatcag
     3241 aagaagcctt caagttatgt agtgatttgt tttttatcga cggtatcaaa cgagatcgct
     3301 ggatttggtc tggagatgcg tatcaatcat tcatgatcaa tcaatatctt ttttttgacg
     3361 aggaaatcaa taaacgaacg atattagcat taagaggaca aaatgaaatc aagcagcata
     3421 tgaatactat cgttgactat tcgatcttgt ggttgattgg tatcgaaaac cactatatga
     3481 tgacgaaaga ctatgaattt ttacgcatcg tctaccctaa gatgcaatca ttaatgagat
     3541 acttgatgga gcaaacgaat gaattagggt ttatttatgg gagagcaaaa gattggatat
     3601 ttattgattg gtctgaaatg gataaagaag gaacgcttgc tgcagaacaa gtcttattac
     3661 tgaaagccta tcattccatg atcacttgcg gagatatctt agctgaaaca tatggagaga
     3721 acgaaaatta tcatactcat ctctatcaag aaaaatatca actattgaaa caaaacctat
     3781 ttgactactt ttgggatgaa gaacttggcg cttttatcga ttgctatgaa tctgggagaa
     3841 gaaatgtgac gcgtcatgcg aatattttcg ctgttttatt cgattttgct aacaaagacc
     3901 aacagcaatc gatcttaaag aatgtcttat tgaatgacac tatcacacag attactactc
     3961 catatttcaa attctatgaa caagatgctt tatgcaaact cggacagacg aatatcgtct
     4021 accaaacgat tcttgattat tggggaggga tgttatctca tgatgcggta actttctggg
     4081 aagaatacga ccctactcaa acaggaaatg aaaagtacag tatgtatgga gatccttatg
     4141 gaaaaagctt atgccatgca tggggagcaa gcccaatcta tcttttagga cgctatttta
     4201 tcggtttgta tccaacaaag ccaggttatg aagaatttat gatacaacct aatctagatt
     4261 catttgaaac actgaattgt acgctaccta tcaaagatgg aaagatttcg cttgaaaaaa
     4321 caaaagataa gatcgtgatt acttctgata gaacaggcgg aacagtgaaa atgaatggta
     4381 aaagttatcc tcttctagca aaacagccac ttgtcttaac aagctattca aaaataaatg
     4441 cataaaacaa aaaactgctt gcgacatatc agaagacttt aaatcttctt ctgatattgt
     4501 ccaagcaatt tttattttga agtttgctta attctgttct ttagaaacaa tgtttgttct
     4561 ttttccggca aaaagaaagc tagataatac gacaagtaaa aagaaagcga tgattccaac
     4621 tgtcaaggct atttgaccgt attctcctag tttcccaagt acaagtcctg gtactagata
     4681 atcagtatcg gcaaatgtag aagaagcgat tttaagatct cctaatactg gaaggataaa
     4741 taatggcagc caactaatca ataagctatt gaaagcagaa cctaataccg cccctcttct
     4801 accgccagtc gagttgccga agacaccaga agcagctccg cagaagaaat gtcctactac
     4861 cccaggaatg ataactgtag tttgcaagcc tatcatagca aacatggata gtgttcctac
     4921 aaagaagcta acaaaaaagc caattaatac agcattaggt gcatatggaa aaacgatagg
     4981 aacatctaat gctggtttag aatcgggtac gagtttttta gcaatccctt ggaatgctgg
     5041 aacgatttct gccaacacca ttcgaacccc ttgaaggacg ataacaaacc cagctgtgaa
     5101 tgttccagct tggatcagtg caaagattac agcgttgtct ccattgctta gttctctttc
     5161 aacaaatcca ggaccagcta acaaagcaag tgcgacatat acgatcgaca tcaagagcat
     5221 gatcgataca gtagaatctc gcaaaaagct gaaactttta ggaatcttta tgtcctctgt
     5281 agaacggtct ttgtttccaa aaaactttcc gatccatcca ctagaagcat aaccgatatt
     5341 tccggtatgc cctaacgcaa cttgattgtt tccagtgatg ttcttcataa acggttgaca
     5401 aagagcaggc gtaatagtta atagtgttcc ttcgaagacc ccgcctaata agatcatcca
     5461 caaatttgca gtaaaccccg cgcttactaa aatgactgct gttagacaag aaacataaag
     5521 cattgcttga cctgtaagaa aaatgaattt atatcttgtg aatctagcaa gtaaaatatt
     5581 cacgatcatc cctactagca tgatcaaagc agtaggtgta ccgtattgtt gcaaagcaat
     5641 cgccactact gcttcattgc taggaactac tccttgagta tgcagagctg tttggaacat
     5701 cgaagcaaaa ggggttagtg aattactgat taaaccagca ccgccgatca gtactaggaa
     5761 tccgacaaat gttttgatag ttccttggat cacttctgaa aatttcttct tctgaagaac
     5821 gagaccaagc agtgcaatca aagaaacgag gatggagggc tgactgacga ttgacacaaa
     5881 caaattcaac aatttatcca ttgatagttc ctcccttgaa tcatttttat tcttttgaca
     5941 agatcttcgt tactttttct tttacttctt cgctgtcaat gatcgattgc aaaggtacga
     6001 gcttttcatc aggtatagaa gttagcgtat ctttcaaaga atgctcaatg aagaaataat
     6061 cagccatatc gctagataca cttccaacat ccgtatgact tacttcaaac tcgtccacat
     6121 ctgctccaag atctgcaagt gcttgtttga tgttcatctc taccataaaa cttgttccta
     6181 atccactttg acatactgca aaaattttca tatcattcgt ctccttttct tatgatcgct
     6241 aatatttctt cttttgttga tgcgtgtaat aaatcttgaa gattttgttt attagaaagt
     6301 agctttgtta aatttgccaa agcccgaaga tgtgcctcat tatcgatagc agctaaacag
     6361 atgaacaaac taatgggatg ggctggatta tcaagcagat ccacaggttg ttgaagtttc
     6421 agcaaagaca tccctacttc gtttacgcca tcttctggac gagcatgagg taatgcgatg
     6481 tgatcgccaa tatggataaa cgcaccatac tgctccacgc gctcgatcat agctttgata
     6541 taactttctt ctattttatt ttctgataat agcggctgtg cggcaaatgt gattgcttct
     6601 tgccagtttt ttggttgatc cgataattgg atcatttctt ctgttaataa ttcagatagc
     6661 ataggacgtt gatcctcctt cttttctaaa agtttgttca ttttacgatt taatacttga
     6721 tagagctttt cttcgtctac gccttctttt aattcgatat aaggcagtag cgccgacaac
     6781 aattcttggg cagaaggcat tgaaaatcct ggtatcagcc aatctctttg tacttgattg
     6841 atcagctgat ttttttccaa gtcagtcatg attggatgca gaacatagag agactttttc
     6901 ttatcagtaa gtgtgagagg tacagtagaa aaaataatat cgtaagaagt ttcgtcaacc
     6961 tcgtgtaagc ggtcagctga attagtctct gtaaaaaaga tcatcgggaa taattgctga
     7021 agttcacttt ttaagatcaa tgaagagcta atgccacttg gacagacgat cacggctctt
     7081 atttttctat ttctttcttc tgcgtccgct tttctgatct ctccgccaaa tagtatagtg
     7141 aaaaaaccaa tctcttcttc agggatgctc ttccctattc gtctttctaa cggaagtaat
     7201 gcttttcttg tcatttcgta gatggaagca tattgatgct taatttgatc gatcatcaca
     7261 ttaggtaaat aaaaagaata ggaaatacga aaataggcag ggaccaagtg ataaaagaca
     7321 ttcatcaata attcacgaaa cgaagcaaat tctacggcca tcaggaattc catgcgatgg
     7381 atgacttctg aagaacattc cagcaaaaaa tcaagtgcag gctcctggac atctccttca
     7441 acgatcgatg ctaagcaaag agagaaaaat ataatctccg aattttgatg ttcggaatag
     7501 ccaaggccag tcaatacttg tcgactaatg tcagtgactg caagagaagc caacagttca
     7561 gcatctttta ccagttccat ggatgttttt ccaattcgaa tggttgtcaa actcaaataa
     7621 taagctaatt catccattcg atttggtgcg aatcgaagat ctgatagtga aatcgtttta
     7681 tctagcaaat ctctaacttt ggcgtaataa tcaatggaaa gggttgcggt taaatacgtg
     7741 agcccaaatc ttccagcaag tgactgcatc aaacgagcag tgcgattttt tgcttctctt
     7801 cttaatattg cttctgaccc tagcagccta aaaccagttt tcctagtata cgacagctga
     7861 caatcttcca cttctaattc atttcttatc tttttgatat ctgacaggac tgtcccttta
     7921 gaaacctgaa gaaaatcttg aaaatgaaaa acagaaacag acgttgcagt cgaataaacc
     7981 atcaaataaa gaagatctct tcgctcttgt tcggtataca cagcttcaaa tcgagtaata
     8041 tccaaaaatt tttctgccca gtcgattgct cggaatttct ttatacggat acgggtatca
     8101 tccacatatt cggcaaaagg agttaattgt ttgatcgcct cgcttgtatt tacttcgatg
     8161 atctctgctg ctttttctag actgatttct cttggtgttt tcaggagata actaattttg
     8221 tagttcacag cgctgttcac ctcctactta tattctagta tggtagcgct tttaaatcga
     8281 ggggtgtttg gtacaactta cagttggatt ttttttacga ctgtaccgaa cgatttctat
     8341 cgaatggata cagaaaaaaa gcaggtgacc aaatgtctcc tgcttttatg aattgatttc
     8401 gtccgatcaa tgatgatcat gtttattttt tgaggtcagt tcttcaaatt tttctttgac
     8461 atcttcaacc gctttttcga tatctttttc aaagtgattg ggctcattag gctttgtttc
     8521 ctcttctaca gaatttgaac cagataaagc tgtttcttta ttttctgttt caccagagtt
     8581 tgtgccagct aatgattctt ctttgtcttc tgtttcacca gaattagttc ctgctaaagc
     8641 cacttcttct tctgttttag gggccattga acctcttaat cgtttgtgtt tttcaccgtc
     8701 ataatttgtc atgttggttc ctcctagtag ttctatactt tttcatcgta caataagaca
     8761 gtttatgcgt caaaaaacaa acccgattct tacaaatatg tcatattttt ccttctgatt
     8821 cttgaatgct tttgattcct tcttccaaaa ttttcatttg taagattgtt tcttcatctt
     8881 tgatgacttt ttctgcgcca ttaactaatg tctcgtaaat cccttcgtag acacggctgt
     8941 aatcaccaac ctcggaaatt actttttctt catgataact cccttgttca tctatatagg
     9001 tcaatacacc ataatgttcg ggcaaatcga taccaaagtc ttcatgatca ggcatgtaaa
     9061 acattttcag atgttcttct tgtcgatctt ttgtttgttt cacgaacatc cctttttttc
     9121 cgtaaacgac gaaacttgga cgttctttga tgcggaaata actggatttt atcgaaattt
     9181 tcatatttcc atagaaaaaa tccagatcga agtaatcatt cattcgtcct ttccctaaaa
     9241 gttgacgtac atcatagctg atatcatctg gttcgccgaa ataagaaaga acttgatcta
     9301 ctgtatgaca agcatgtcca tataaatagc tgttcgcagt agaaaattca tgcgtatttt
     9361 ctggtacttc cggtctgaaa taatcataat gcatttctac ttctaaaata tcccctaatt
     9421 ttccgctttc gattactttt tggactgtca agaagtcaga atcgaaccgt ctgttttggt
     9481 aacattggat aaatagacct ttttcttttg ctaatgcaaa taactcttct gcttcctttg
     9541 acgtttctgc aaaaggtttc tctactagta cattcttacc atgttcgagt gccatttttc
     9601 catattcata atgaaattga ctaggtgtag tgatgacaac taattggatc tcaggatcat
     9661 cccaaagatc agacagctga tcagtatatt cgatgtcttc gaatgttttc cacactggtt
     9721 cagatgggga aaaaattttt ttgatattga ttttgttcgt aagttttctt gaaaaaggca
     9781 aatgatagcg atttgtactc tttccattgc caagataagc gattgtaagc atgagtggtg
     9841 cctcctttta ttgatgtttt cagtatatcc tagaaaagcg cagaaagtat ttataatagc
     9901 tattctggta aaaaagttca gaaaaacact tgttatcatt cggtttggtg aaattaatgt
     9961 tcaaagtaag gataaaaatc aaaaagatag tagcggaacc aagtgtttat cagtaagata
    10021 gaatttggag gtcagatcat gcttattgaa gaaaaactaa caaaacaaga gttgtttacc
    10081 acgacagaga aacggattgt cgattacatc cgtaggaata tcgaagctgc agtctacatg
    10141 acgattgaag aattagcaaa agctacgtat acttcccatt cagctatcat tcgtttatgc
    10201 aaaaaaatgg gattcagcgg atttaaagaa ttccgtttcg agctggggcg ggaagttcat
    10261 caattgattg ctcagttcaa tcaaacgatt gatcctaatt ttcctttttc aagtgaagat
    10321 agcactcagc aaattgcaaa gaaaatggcg gatttatcga tccagtcaat aaaaaaagca
    10381 caattgcaga ttgaacatga agatttagat caaatcggta aagtattgaa caaggcacag
    10441 cgaatctttc tttttgcaaa aggtgattct caaatcacgg caaggaaatt ccagaataaa
    10501 atggtcaagt tgaataagtt tttgatcatg gctgaagaat acagtgattc ttcttggaat
    10561 gcagccaatt tgactagcga ggattgcgcc atttttatta gctacagcgg tcgcatccat
    10621 cattatgaac gaataatgac ctatttgaag catgtagagg cccctacctt gctgattacc
    10681 ggaaatcagc attcagaaat ggccaaacag gctgatatgt gtcttgttat atcgcaggag
    10741 gaatatgatt ttgctaaagt agcgactttc tcctctcaaa ttgccttcga ttacgtatta
    10801 aatacgttat tctcagtgat atattctcaa aattttgaag agaatatccg caacttgaaa
    10861 aacaaacaag aattgatgca aaaaggcctt cttaaagaat atcggaaaac ttaagaaaag
    10921 atcctcattt agtaataaga aagatagtgt ttgataggct ctctttgata tcatcataaa
    10981 aacaggctga agttttcttt ttcaaacctc agcctgtttg tttacggaac gattcgaata
    11041 gatatattat agaaatgagg aatagctaac ttctgtttct tattttgaac gattgacgaa
    11101 aaagatatat aagaaggagg aaaatcagta tacatgcagc taacaatcca tacaggaaaa
    11161 atcgtttgtt ctcggatact ttctgtactt ctttttgaac ttcttttgca tgataaggaa
    11221 cccggtgccc tctgacaagt aagcgatgac tgttgaccat ataaggtgta caagttagga
    11281 gtgtcacgaa atcctgccct ttttcgattc tcagatcttt tgtatcagtc ggttctaccg
    11341 tcttgatctg atcgacttga taagcgagtg tccgtccatt gatttcgata tagaaagaat
    11401 cacctaaagt cagtttcggt aagtctgtga acaactttgc ttgaggcagt cctcgatggc
    11461 tggaaataac ggcatgtgta ttttctcccc ccgtaggata agacgttcct tccaataatg
    11521 aggcaccttt ttctagcagc acagtattcg tttcatcaaa aattggcaaa tggacattga
    11581 tctcagggat cgtcaaaata ccgatcgtat gtttctcaaa ataggttcta tcctttttcg
    11641 caggttcttt tttcttagta aagggatctg cacctggatt tccaccttct tttgctaatt
    11701 gctggttttt ctttgtcatg ttttcttgga ttttagccat gacttcttca ttttctttga
    11761 ccgcttgttg ctgataatga gagatgatct gctgatccag ataattattc agcgcgtcgc
    11821 taacaaatgg ataagctagt atccctattc ctgagagaag caagaaaagc atcagcagat
    11881 ctagaagttt ccttttcttt ggtttcattt gtgttctcct tgtgatgact gtttgataat
    11941 aaaagaaaaa ggagcatttg cgtaatccgc tgctcctttt tccaattctt gcaaaaagac
    12001 taaatctgac tgtgcttgcg tctagcaaag tacagtccag caatcagtag aaggactact
    12061 cctgcaccga tatacacata gattccctta ccgcctgttg aaggaagtgt acctttgtgt
    12121 ttatttggta ttttttcagg agaaatcaac tgtcctgctg ttacataaga ttgttcatcg
    12181 atagtaaatg ctacacggtt tgttaatgga acatattttt ctggcgcttt cgtttcttcc
    12241 agatagtacg tgccatattt tagacctgtc acatcgatta aaccgttact tgtggttgta
    12301 aaaaccgttg ctgattcttt cgccgatacc cagctgacgg cttttgtgga tgggtcgatc
    12361 gataaatatt tcgctgtgtc actatcttga tcacgaacga cgaattctgc tccagcaagt
    12421 gtttggtctg atgtaacgtc accatctact ttaacaaatc gtttgccccc agtaacgaca
    12481 tcgactgacg gtggtgtttg atcatttgta tgcccgttat cgacatttgc ttggttagaa
    12541 aatcctttgg ttggatctgc tttttcgttc aaatgcatat agtaaacgaa tttcaatgta
    12601 ccgccaggag ttaatgaagg aatataattc ggatcaactg aaaccgtgaa tccgtctgtt
    12661 tgctcagtga cagaataatt tactgggtcg atttctttat ttccatcata taaggcataa
    12721 gcatacgttc ctgaagaatc attatcaaat gttaaagcag cgtcatgagt atcgataagt
    12781 ttgaatgttg tgtacttgtt ttgcgttcct tctttatcag cgatacctaa tgggatattg
    12841 acagaaatct catattgaat tttttcaccg attgcgacat cttttccatt caattgaggt
    12901 gttgttttat caactttaga cgtatcattt ttgatggtct tttctactgg tgtttggcta
    12961 tttgccagga tctcaaaagg cgtgattgtt tgcttttcaa tcatttccgc attatcagga
    13021 gcttttactt cttctaaatt atattttcca acttctaaat gattaacgat caactggcct
    13081 ttttcaatag atgcctcggc aaagtcattg ttgccgatgt cataagaatg accagtaatg
    13141 aaatgtttgg ctttggtttg atcagttgtc caagtgtaca atccatctgt gacactttgg
    13201 atgtattttt tgacgcttgg tgttccttct tctttagaaa taataaattc tgctccattt
    13261 agtgcttcgt tttcggcagt accgattttt gtaactttca acgttccatc attaccgact
    13321 gtatttttag ggtagagatg gatagtatct agttcttctg tcccgtattt ataagagcca
    13381 tctgcttgtt tgatcatctc atatacaggg aaagctaaaa ccatgtttgc ggcagctgac
    13441 actccgtctt ttggttcttc tttgatcgtg tagactgcat ctttcccatt ttgtttttta
    13501 ggtaaagata aagtgacatt tccatcagca tctgtcgttc ctgaagcaac tggtgtacca
    13561 ggagtcaaag actggactgc ttgttttgct gcatccacgg acgctccttt atctcgttgc
    13621 gcataaaatt cttgagtgac gttataaact gaaaatgaaa tatcggctaa tccttggtat
    13681 tgatcgaatt cgctcatttc tttcccgctg ttttggatta aaggatcggg taaatcagtc
    13741 attttctttt tatgaaggat gacttgagct gctgtctcct ctgcaaacac tttttttgcg
    13801 ccgaagctgt ttgtgagtaa tggtaaaata aggaaaagga ctcctaacat aacgtttatt
    13861 tttttatgat ttttcatttt ttcactcctg ttcttttttt gataaacaac cagattgcaa
    13921 agcttgcgaa taccatacca acaacgccta aaagtgtatt tttcgtttcg tttgtttttg
    13981 gaagaaatgg acggctattt tcttttttat tgactacttt caatatactg tccgcctctt
    14041 tatcctcaga aagaaaagtt gaaatggtaa acggaatttc tgtttcactt aaaatataac
    14101 cagaaggtgc ttcgatctct tttaatcgat aacttcctgt tttcaacccg gaaattgaaa
    14161 aagcgccatt tttatcggaa atcaggtgaa gcgcatctga ttcgttcttt gtccacttgt
    14221 atccgttggc tgtttgctgg aggtaatttc ctgctttgtt tttaataagg aatactgcgc
    14281 cagctaatcg tttatctggt ttatttgtct cgattttgac aaattgctta ctccctgtat
    14341 atacagagac tttctttgta ctgacaccaa aaccaaaatc cagtgttccg ttgttgttga
    14401 tttccttgtt cgcctgtgct gtactgctta gctgcatctg ataagaaatg gtcagttttt
    14461 ttccagcata tttttccaac gaagggatcg aaaataaaac atcaaatccg tgaggtttct
    14521 tgtgtatgac aaagccttct gtcagctttt ctccattcga tgaaatcgtt aaactattag
    14581 gtaaaaacgt taatgcttca tccgcactat ctgacaattc gaacttttga tagtcaagga
    14641 tatttacagg aattgtcgta tgcaaggaat aagtgatttt ttcaccaatc gtaaaatcat
    14701 ttctaagctc ttctaatacc ttctcaaaag gtggatcagg gtagtcgttt tcttcatttt
    14761 taggatacaa atggatagtt gaaagttttt gattgttttg tccatgtaca ggaagaacaa
    14821 ctaccatatt ctctgccttt tctttgacga cttctggtac tttggattca atgaaaagat
    14881 acgctttatc tcttttttct tgatctttag cggccagaga aaaagaagcg ataccatctt
    14941 cgttgttaag agttgtagtt gttgcttctg cggtaaacat accggaagac gcaccgtttt
    15001 ttgcgatctc tgcttgtgct tcttctaccg ttttgccctt ttcccgtaga tggtaaaaag
    15061 aatctgtgac atcataaact tggaatgtga caccatttaa tcctcgatac gtttgtaata
    15121 aagctttttc ttgtccgtca tttggatgat ttttcggcag ttgcccgttt gggaaaagca
    15181 atttgtgtaa agtaatctga accatttctg tttctgtttc tgttgctacc tgagtaaaag
    15241 ctggtttaaa tagtaacaag aagagacaca tactaagcca accaagtttt ttcatattta
    15301 gctgccccct ctttcttttt tcaaaaaaag ataccaaata gaaaacgcac aaccaatcat
    15361 ccctactaga tagattccta aacggccaat tcctcccgtt tcaggtaatg gtacttttgc
    15421 ctgattcgtg atgtctaaag aaatctggtt gtttttggct cctggtgaca gaacagattc
    15481 atgatcttgt ccatccactt gaattgaccc atcttcgtgt ataactatag taactggctc
    15541 ttttagacct tggtatcctt ctggtgtaaa agtttcggtc agcgtataag ttccaggttt
    15601 taaattttcg aatagaaagg tatctgtttc ttgtccatct tttggcgatt ctaagtccat
    15661 ttctggtccc tgcagtctga attttgctcc ttttaatgtc tgtccgttgt cttctttttt
    15721 gtgtaccgtt agatcaaacg cttttagtcg attttgatga gtgagcgtcc atacattatt
    15781 tgtcatctcg gtattttttc ccgtgtaaat gatttttcca tatttatcga tttggaattc
    15841 ataatttccc ggcgaagtat catatccggc aggaccagct gtttcaacga ctcgatattt
    15901 accaggtgtg agtgaatcaa aactggcaag acctttttca ttcgttgttt ggctgtcaat
    15961 tggctgataa gtaccatttg cttctttttt ctgtagggaa aatgctgctc ctttaagatt
    16021 tatctctttg tccgtccctt gcatcgcata cttacgtact gctacaggaa cttcaacaaa
    16081 aggattttca atagtcaact ggatcgtatc atcggaagta gtgactgttt ttccatcaat
    16141 ggttaccgta ccatcagaag cgatcttgat ttcccaagta gtctttttgc ttaatccatg
    16201 cccttctgga gcttttgttt ctgtcagcgt ataggtcatt tctttttgca atttgacatt
    16261 ttctggaaga gaataggttc cgtcgccatg atctgttagt aaagtatcaa tagaatctcc
    16321 tgttaatttg aaaacagcgc caataagatc cttttcacct gtagaggaat ttttcgttat
    16381 tttcaagttt aacggtgtga attgtttagt atttttccat gtcattgcat ctatttgttg
    16441 agaatcgtat cctggtacag gtaattcttt gattgtttgg taactgaatg cttgaccttg
    16501 attattgtat tgaggtagtg ataatatctc ttgataactt tctccgctat ttgcagataa
    16561 tttgtcaatg tctgcacgtt cccacgtatt tgttgtatct ttagctggtt taatgattcg
    16621 aatatatccg tttttccaag ctgcagcatt tgtcgtatgt tcccgttgaa tctcaaaagt
    16681 aacttgatct ggacgatcag ctagattgtt gtcaaattct tcccataact ttttgatgtg
    16741 aagactgact ccgggagctt tagcagatgg tataccgaat tcagctaatt cattggtatc
    16801 aatgtttggc tggaaagttg tcctgccgtt catttgatac cagaaatttg gatggaagtc
    16861 ctcattttct gtttggattc tcacttggta atggatttgg atttcttggt cttttcctaa
    16921 atagatctga ttgctcttga tggtatttcc ttctatggaa atagatggag tgaccgttgt
    16981 aggacttgtc cccacactct tgactgaaag tgtaccaggc tgataaatga atggttctgc
    17041 gattggatca tttatttgtc cattgcttac agttgctgag atctgtacag cttttttggc
    17101 taggtattca gagatatctg ttgcatgatc agctgattca tagtaaagat cgcctttttc
    17161 atctgatgaa accatttgtc gcatacgaga ctctacttct gcttttgaga gaccagctgc
    17221 cggatcgctt tgaagttgga tgccaagacc atgtatttcg attcctcgtt ctttgagtgc
    17281 catcgcttct ccgatggttg cgataaacgt actgtcgatt cttctggata gattgttttg
    17341 gtcaggtgca tcatagattc ttgagattag agaagtattt cccggccgat cttgcgtatt
    17401 agaaaactga gttccgtaat aattgctgct tgattgtgcg tgtacccgct gtactttata
    17461 ggaaaatgtt ggtacaccat ccgtcagcaa aacgatcact tttttatgtc catttggaac
    17521 ggatagcatg cttcctgcat ctcttagtgc tttttgtgta aaagtaccac catttgtccg
    17581 tgaaggtgta atggatttta cttgattttt cactgaatca aatgaaccca tctgtactgc
    17641 accgttactg tagctatatc cttcgcttga gtagccgaca tatcccatat tgattttgtc
    17701 tgtgataccg ctatctgcta aagtatcgac aaaacgatcg acaccaatct ttacttcacc
    17761 gatccgatta ttgtcgttca tacttcctga ccagtctacg accaatacga gatcaagagg
    17821 cgtgatatct ttttgtacat ttccacgagc attcaaataa acatcaaaca atccgggtgt
    17881 acttgtttcc tttgcatact ttcgtatcgc atagtctgct tgattaccgg ttcctccata
    17941 ttcgatatac gaatgggtcc gatcattagg atctccattc caagaattta taccatccca
    18001 ttgatttgtt ccgtttttat tgccttgatg atcaagaaca tttgtatttc ctgtaggttg
    18061 ccaactggct ttaggataag tgccctgatc atctgttgta tattctgtag gaatcgtttg
    18121 attagatggc acactcacag ctggcaagga taatgtaggc aatgtaaatg ggctgacaga
    18181 attttcgttt tcttttgatg attcttcttc tatttcgtct ggctttggta ctgacacatc
    18241 tgtatctttc ttattttctg tctcatcaat aatgtcatat tctttattat cgacgatcag
    18301 cttgtttgcc tggctgtggt aaagtgctgg atcgattgga aaactcagat cgatagtttg
    18361 tttagtgttt gatgagattt ccaacgtaat tttcccgcta tttacctgat aagcatagcc
    18421 atctccttct tttctttcca catcacttaa gtaattttcg actagaccaa tttctttttc
    18481 tatcttttca gcactggtgt tattcgcatt tagcttgatg ttcagtgctt tttttccaga
    18541 ctgatccgaa atgacttcag ctgaaattgt ttctaaaact gttggatcat ctgtagtgtc
    18601 tgctattgca gataatggca caaagtttga taaactcaat attatcagca tgaaaataac
    18661 tttcagtttc ttccctgtgg tcgtcatttt tcccctcctt tttctttttt tctgttttga
    18721 cgtgaatagt gaaaattatt aaaataaaca ggcgttacat aattttattt attttaatat
    18781 gataattatt atacgaaggg cgctaaaggg aatcacacca attttcatac caattggttt
    18841 catttatgaa gttgattcct tcgattaatg agcgtttcat tgaaagaaaa gggttgaggg
    18901 agaaaaacat atgatcaggt tgttggaacg aaactataga aagatggaaa aaatcattcg
    18961 aattctgtcc atttctgaca agtggatgac taaaaaagaa cttgccaaac gaattggtag
    19021 ttcagaatct acatttgtaa ggtatatcga agagatcaaa atccgctggg gaacggatgt
    19081 aacgattcaa tcttcacaaa aattggggta tcgtcttgaa catttgaatg taccagctta
    19141 tttaaatgtt ctgatggaaa tgtcgcaaag ttctacaaac agtcgtttat taaatgagat
    19201 tattgtcaat ccgggtaaaa cgattgagta ttattgtgaa aaaatttcca tcagccggtc
    19261 ttcttttaca cgtaagttga aacaatgcaa tcaaatttta gaaagctatt tattaaaagt
    19321 gatcgttgac caaggttttc agttaactag ccttaaaagt gagctccagt tgcgtatctt
    19381 tgttaccttt ttctttttaa gttactacgg aagacgcacg ttgccatttc caatcgaaaa
    19441 aaagaagatc aaagaaatta tggtcaagaa taattgtcag atgaatttgt ttcaggaaga
    19501 aagcccatat gagcaatctt tctttattat gtattttgtt gtgcatctca tgagagaatc
    19561 ccaaggaact ccatttgaat ggaaagctgg agactcttat gtacagttga taaatatcga
    19621 taaaaaagac ttcttaatct tgaaaaatct cttaggaaaa ttgaagtaca gcagctatat
    19681 aaaagtagtg aactattttg tcaatacgat tttttcagaa ttttcggaga aagaaaaaaa
    19741 tgaaatcagt gagcagatac gaaaaaatat gtcctactgt tcgttttcat ccgctgattg
    19801 caaagaaaaa aacgaagaat atgttgttca attattaacg caaatttatt ttttctcccg
    19861 tattttccca tacgatacga ctcatttgac gatccgtgca acttactttg cacagcagat
    19921 gcaacaccgc cagatacgtc tatatgctgg atttcgtgca gaacttcaat tttattccac
    19981 tctactgaat cagaatctga tttattattt atctagctat atcttttgga tgcttatcgg
    20041 taatcctgaa attcaccatt atacttctga taaaaaaata ctgattatca gcgacttaag
    20101 tcttcgacat agtcaatata ttgaagaata tatcagtgat attcttgctg tgcataaaat
    20161 tcactctgag acaactgcta ttactgaaga tcagctgtcg aaatacaatc ttctcgagta
    20221 tgatctgatc gttacgaatc aacccatttt gaatgcccat gttcctcata tattgattga
    20281 tgatagtgtg tcattcgcaa atgaagaaga actcatccgc ttattcgagt tatgaataat
    20341 caaaaaacag ctatgtataa accaccctga taaaaatttc aggacaaata cacatagctg
    20401 tttcttttta ttgtattcct tcagcaatcc aacgagattt atagcgaaag tcttttaaca
    20461 actcttcttt tgtccaatta tttgcaatga gatccgtcaa agtgttcatt acgagggtca
    20521 tggaaaagta taggaaacta actgcttctt ccgacgattg aatggagaaa tgattgattc
    20581 ttaataattc cagccatttt tcgttcattg gagaatcagc cgatagttct ctacgtttgg
    20641 ttgtctgctg atcatttcgc tgggttaaca agtataacct ttgccaatag atactttcgt
    20701 gatctaagtc gcagcaccac tctaaatatt tttcgatccc caaaaagaaa tgttgtttgt
    20761 tttcgctaat gaattttaag atatcttgat ggactatgtg agaactgctt ttgatcgtat
    20821 acaaataagc atcttccaaa tcttgaaaat atttataaaa agcaccgcgc gacatcccca
    20881 tcttgtcaac gatttctgag actttgactt ggctgatgtg ctgtgagcaa aatatctcca
    20941 gtaataggtc atcgattctc tgtttctttg cgttcgataa attgaaaaaa gtttcttttg
    21001 gcatccacat ctctccttta tctctgatta tacgtggata gcccttcatt gtcatgaagg
    21061 attcattcct tctttttctc ttcttctgtc aagtacccat cttccattcg ataaacgcga
    21121 tcactccatt gaatcatacg gacatcatga gtaaccataa tagtcgcttt ttgtttctca
    21181 tgagcttctt taactagtaa tttgacgact tcatatgcat gatctgtatc caaactagct
    21241 gttggttcgt ctgctaagat caagcttggc tcattatata aagcgcgagc aattgccact
    21301 ctctgtcttt ctccaccaga aaggtcttta ggataactat ttttaagtga ctggatatct
    21361 aaagattgca gcaaggaatc gatttttgtt gaatcttctt tttttgaagt aacttggtca
    21421 actagttcaa attgttcctt taccgttaaa aaaggaatca aattagagct ttggagaata
    21481 aatcctattt ctttaaagcg taaatctgct cgtttttttt ctggaagctc agttagtgag
    21541 tgaccgttga tcactatttc tccggaagtc ggtgtttgca atcctcctga aatggtaagg
    21601 aaagtacttt tacctgaacc agaaggacca atgatgctga cgaattctcc ttcctttaca
    21661 taaaaatcaa ttgctttcaa agctgagacc tttgtatgac cagaaccaaa tgttttggta
    21721 acttttttca tcgttaatat ttttttcatt tttattaacc tcctattgct gtaattgggt
    21781 ctactttggt tatcgtacgt atcgaaaaca atccgcctag cattgcgaca atgatcaaaa
    21841 tcccactata tagcaaccat tgtgaccaaa aaacagcaaa aggcatcgcg cttggtaaaa
    21901 tcagactagt caaataagca aacaaaagag caagcagaac gcctaccacc ccaacaataa
    21961 aggactgtgc tactaaagat ttagcgatga agaaattttt gatcccttgt gctttcatta
    22021 ctccaaaaat agcagtcttc tgaagtgtca tcacgtacat gaaaataccg accacggcag
    22081 caaccactaa gaacaaaaag tatatcatcg catttaaggt catattttgg gcagaatagc
    22141 caggaatact ttcaatgaat gtcccaatag ttaataactg tagacccttc gatgcttttt
    22201 ctttcgaaat tttagctgaa tcctttatta cgatcccatt gatcggctgt tcttcttcag
    22261 aagcaaacgg ctgttgtcca tacttcaaag cagtccacgt atctagggac gcatagatga
    22321 cgggagaaac tgtgtaataa gtttcaggaa agataccgac aatcgtcaaa ggatcgtcgt
    22381 aactgccaat tttgatcgta tctccaattt tatagcctga atcagccaga ttttgagaga
    22441 tgatgatttc attctttgtt ttaaattcat gccctttcgt gagttttggt aataaaaatg
    22501 cttctttcgt tgtgccgaaa actgtaacat tttctttgtt ttttcctttg attgctccgc
    22561 tgtacaatcc gattggtgct ttctctcctc cttcaatgta tttcagatct cctcttgtta
    22621 attgtgaagc tgcaaatact tgatttgcat cctccgacaa aacaatctct tgtgcgtccc
    22681 aatcatcgat tgctttttta aactcttctg aaagtccatt cgctagtcct gacaacatga
    22741 atacgacata cgcaatcaag aacataatac cgacgatcaa gccatatcgt aatttagaat
    22801 agcgaatttc tttgatagct agaaacattt gtttttcctc cctatgatag attgatagtg
    22861 acaaaatgtc acttgttgaa tagtgtaact ctaaagtgac aatttgtcac ttgtgttgtt
    22921 tttttcttat caataaattg tctaagacat ctttcgataa aaccttaaca tgagtcataa
    22981 aaaatttatt taagccttat ttatgctttc catgataaac taaagtgacc aaatcaatga
    23041 ggtgggaaaa tgattaaact attagtagta gaagacgata aagtattatc caataatata
    23101 aaagaaatac tcgctgcaat cggcgagata acacaagttt atgatggtag tgaaggactg
    23161 ttcgagttgg aaagtggagt ttatgatctt gcaatcttgg atttgatgat gccggaaatg
    23221 aatggatatg aagtgttagc aaaaatacgg aaagaaaaaa tacagactcc agtattgatt
    23281 ttgactgcaa aagatagttt ggaagataag attgaaggat ttgaacgagg tgcagatgat
    23341 tatctaacaa aacctttcta tcgagaagaa ctgatcatgc gcgtgaaagc attattgaaa
    23401 cgctcgctag gtctctttga tgaaaatgtt ctgaagtata aggatatcag ttgtgattta
    23461 aaaagcaatg aagtcttgta taaagaacaa gttttgccca tacaaggaaa agagtttgaa
    23521 ttgctcgttt attttttaca aaataaggga atcattttga caaaagagca gatatttgac
    23581 cggatctggg gatttgattc tgatacgacg attacagtta ttgaagttta tatgagcaac
    23641 ttacgaaaac atctgcgaag tactggattg gataaagaaa tcaaaacctt acgtaatgtc
    23701 ggttatatac tgcaaagggt gtgatgcaat gaatggagat attacacgaa agcaacgtag
    23761 tcgctttttt gcgacaaatg tagcagcatt tgcttttatc ttttttcttt tgggattcat
    23821 tactttacag atattaaacc aatctgcata cagagaaaca gatctgtcat tagagaaaat
    23881 aaattttgat tctcgaacag tacaattaga gatacaaaga tatcagcaag gcgatccctt
    23941 tttaaacccg actcaaacgg aacaaaacag aacaaacatg gaacagccat taatggaacc
    24001 atcaaatgac cgttttaaca ctcaagtgat cttgtggtct aaaaatggtg agatattgaa
    24061 taaagcagct attggtgggc gtttcaacca aatcaaagaa ttaaaaatgg atacgaaaaa
    24121 tctgaatacc atccaaaata ttgaattaaa tgaagacagt ggctcatctg ccctctcttt
    24181 ccgttcgatt acgaaaaaag cggagacgaa tcaagatggc gttgcctaca tccaaatttt
    24241 agaaaataca aatcaaatca agaattcatt agcgactttt cgcacgatta tgattttgtg
    24301 tatgattgtt ttttggattc tctctattgg tatcagttat tacttgtcta gtctaagcat
    24361 gcgccctatt attctttctt ggagaagaca aaaagaattt gttgagaatg cttctcatga
    24421 attgcgcaca ccgttaacga ttattcaaaa tagtttggaa catcttttta ccaagcctaa
    24481 tcatactatt attgaagaat ccgaaagcat cgctcaagca ttgtcagaga cgaggcgcct
    24541 cactggattg acttcggatt tgctgacaat cgctcggaat gattctgatc agcagctgct
    24601 ttcaaagcaa atgatcaata cacaagaata tatcgaacaa cttgttaaac catttcaaga
    24661 gatggccatc atagatggaa aagagtttat tctccataac gatgcaacat tagaagtacc
    24721 aattgatgaa aaaaaaatcc atcaagtttt ggttattttg ctggataatg cattaaaata
    24781 tacgaatcca gaagataaga tcattttgga ttcatctgtt aaccataaag aatggctgat
    24841 tcacgtaaag aatactggcc cttctatcag tgaagaagat caagcaagga tttttgatcg
    24901 attttatcgg gaagatcaat ctcgggcgaa agagactggc ggatacgggc ttggcttggc
    24961 aattgcaaaa caggtaatcg aacagcatca tggacagatt tctgtatcca attatcttcc
    25021 acaaggagtc gattttaaag tccaattgcc gttgaagcct aaaaaataat gcttatccct
    25081 aaaaaagggc tgtgacaaaa atcttgtcac agcccttttt tgttgcgaat aaacggtgtt
    25141 ccagaagtag cttcttcgga aataagccga aatttcttta agctgaccac ttctgtcaca
    25201 acctctttag cttacgctta cgatttttta ttgcggtctt caatgtgtac atcaccgctt
    25261 gtgtcaatat ccaattcttc tcggctaaca tcttctgtca ctttttcaac atcttcatgt
    25321 tcttctttat gaatcccaac ttcttcacga ataactgtat gtttgcttac atctacttgc
    25381 tcttctttga ttgggatagt aatcgtgtca tcttccaatt tttcgtctgt tccctgtgaa
    25441 gattgatctg ttactggttt gcgttcgatg acgatttctt cacgtttgac cggtacttct
    25501 accgtttcag tatcgttgac cacatgtttg tggatgtcga cttctccagt tgtcacatca
    25561 tgtgtattta catctaattt ttcttctttt agttgaattg tatcatcatc tagatttggt
    25621 ttgcgatcga tgtcttcagt cttgcttgta cccattgtat cagtctcgcc aggtacgtcc
    25681 atcggatcag ttcctggtgt catattcaat ggatccgttg tattttgaaa gcctgtcgtg
    25741 ccacctacgt ccattggatc tgttccagtg ccagtttcta atccagtccc cattgtgctt
    25801 gctgggctaa cagtatcttg tgtatccatt gtatcttctg ttgtttctgg ctgacgatag
    25861 ccttttactg tgatgacaaa ttttccatca gaaatatctc ctctatattg atataaagga
    25921 tcattttctg gtgtagccgt ctcagaatca taggtatcaa aagaaaaagt atcttttatt
    25981 ttttcccata aagaacggtc ttcttcacca tccatccgat tttcatctgt ttcaacatct
    26041 ataccagaca atccgtcaaa tcctagtcta gatgctgctt catcagtagt ataaataacg
    26101 atatcatctc gttcataccc ttcgttacgc aaccgttcaa ttactgataa tgcttcttcc
    26161 ctcgtactat agcttccgat gaccgttaaa tttttctctt ccatgtaaat tcctcctagc
    26221 aaatagattt atgattggta tattgttact tataaattta ttctatagg
//
LOCUS       DACDPS010000032        25623 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.032, whole genome shotgun sequence.
ACCESSION   DACDPS010000032 DACDPS010000000
VERSION     DACDPS010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.032"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            128..2188
                     /locus_tag="IWJ07_001434"
     CDS             128..2188
                     /locus_tag="IWJ07_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333021.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2271..2840
                     /locus_tag="IWJ07_001435"
     CDS             2271..2840
                     /locus_tag="IWJ07_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9333022.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3015..3821)
                     /locus_tag="IWJ07_001436"
     CDS             complement(3015..3821)
                     /locus_tag="IWJ07_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333023.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3959..5473
                     /locus_tag="IWJ07_001437"
     CDS             3959..5473
                     /locus_tag="IWJ07_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAP9333024.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5876..6859)
                     /locus_tag="IWJ07_001438"
     CDS             complement(5876..6859)
                     /locus_tag="IWJ07_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAP9333025.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6864..7511)
                     /locus_tag="IWJ07_001439"
     CDS             complement(6864..7511)
                     /locus_tag="IWJ07_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9333026.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7596..8228)
                     /locus_tag="IWJ07_001440"
     CDS             complement(7596..8228)
                     /locus_tag="IWJ07_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9333027.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8244..8795)
                     /gene="coaC"
                     /locus_tag="IWJ07_001441"
     CDS             complement(8244..8795)
                     /gene="coaC"
                     /locus_tag="IWJ07_001441"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAP9333028.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8792..9529)
                     /locus_tag="IWJ07_001442"
     CDS             complement(8792..9529)
                     /locus_tag="IWJ07_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAP9333029.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9526..10515)
                     /locus_tag="IWJ07_001443"
     CDS             complement(9526..10515)
                     /locus_tag="IWJ07_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP9333030.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10773..11315
                     /locus_tag="IWJ07_001444"
     CDS             10773..11315
                     /locus_tag="IWJ07_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9333031.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11332..12417
                     /locus_tag="IWJ07_001445"
     CDS             11332..12417
                     /locus_tag="IWJ07_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9333032.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12429..13235
                     /locus_tag="IWJ07_001446"
     CDS             12429..13235
                     /locus_tag="IWJ07_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9333033.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13236..14063
                     /locus_tag="IWJ07_001447"
     CDS             13236..14063
                     /locus_tag="IWJ07_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9333034.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14060..15133
                     /locus_tag="IWJ07_001448"
     CDS             14060..15133
                     /locus_tag="IWJ07_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9333035.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            15561..18257
                     /locus_tag="IWJ07_001449"
     CDS             15561..18257
                     /locus_tag="IWJ07_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9333036.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            18334..18822
                     /locus_tag="IWJ07_001450"
     CDS             18334..18822
                     /locus_tag="IWJ07_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9333037.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            18834..19328
                     /locus_tag="IWJ07_001451"
     CDS             18834..19328
                     /locus_tag="IWJ07_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9333038.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            19349..20158
                     /locus_tag="IWJ07_001452"
     CDS             19349..20158
                     /locus_tag="IWJ07_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9333039.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            20155..20976
                     /locus_tag="IWJ07_001453"
     CDS             20155..20976
                     /locus_tag="IWJ07_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9333040.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            21027..23126
                     /locus_tag="IWJ07_001454"
     CDS             21027..23126
                     /locus_tag="IWJ07_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAP9333041.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            23123..24241
                     /locus_tag="IWJ07_001455"
     CDS             23123..24241
                     /locus_tag="IWJ07_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP9333042.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            24280..25158
                     /locus_tag="IWJ07_001456"
     CDS             24280..25158
                     /locus_tag="IWJ07_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9333043.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            25304..25501
                     /locus_tag="IWJ07_001457"
     CDS             25304..25501
                     /locus_tag="IWJ07_001457"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333044.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
BASE COUNT         8097 a         4457 c         5425 g         7644 t
ORIGIN      
        1 atacattagt ttcaatgttt actgagtaac taattatttt gcaggaggac aatttattta
       61 cacaaaatta ttgacgctcc caattactac ttcatgttta gacaagagta gggaggacta
      121 agaataaatg tgttcaaaaa gacgttcaaa acaactaaat aaacacgctt gtcgtttaga
      181 taaggaacgg tcgtgttctg aaaaacacta cgttaagttt atgatgcgca cgatgctgat
      241 cagtttatct gtaggtgtct atatgcatgt acccgttcag gcaacgacac aaaccatcaa
      301 ggtcgtacgt acctctgttt ctgagaccac tgattcccct atcactatta ctcatacttt
      361 cactgatgca aatggaaaac agctacttcc tgcattgact tcctataata aagtcagtta
      421 tcctccaacg ataaaccact actggttaat tcctaacaaa acattatgga catcggaaac
      481 cggaagcagt gtgccgcttt ctatcttttc cgatatgact gatcctacaa aaggtgatca
      541 gttaaaacag atcactgata ttttgaatca tgctttgaca gatataaagg acggtggaag
      601 cgcacaagtt tcttatgtat atgaaaaaga ttattcacag tttgaaagaa agaaaatcga
      661 tgttctggca gggacaaaag tttcgatatc tgatctggta gaaaggctga agaacgcaga
      721 tggaacacaa ggagatatgt ccaaagtaat ttcaaacaat gggaatatcg tggatacgac
      781 taatacagga acatctacta tttccttgtc ctattttgat ccaattgctt taaaaacaat
      841 gactgcatct gctgtgatcc atgttcgtgc tgatcaaaca gctatcaaag gaaaagatct
      901 tcacttaaga acaggagaga gttacgcccc taaagatctt gttgaatatg taacaaaaag
      961 cgatggaact tcaggaaata aagaagaaat caaaatgaac gggacagtag atactacaac
     1021 tccgggagaa tatcccgttg tattaactta tatagactcg aaaacatcaa atgtggcaga
     1081 aacaacagct gtagtttatg tagaagcatc tgctgcttca tcgactgtca tcactcattt
     1141 tatagatagt caaacaaata aagaacttca agcagaaaca catatgagta gtgtaaatcg
     1201 tattcctgat ttgcgcctac ctgctgaatt ttctgatcga gtgccaaata tagatttatc
     1261 gacttatgaa caaaatggga aagtcacttc tatttctgat tggatgcaag caaacggatt
     1321 agacagaaaa agtcactggt cggagatcct tgaagtactt cactcagaac tgatgaataa
     1381 taggaagcaa tcaggagggg aaattacctt tacttatgtt tatataccag acaatcgttc
     1441 attagaaggt catgatctta ttgttcatcc aaacgaaaaa ataaacaaag aagagttgat
     1501 ccaatcagca acaaattcct taggtaaacc cgtaccgtta aaggatgtat cggttacaat
     1561 caatgaagta ccattggagg atggttattt gaccaaacaa acaggtgatt ttacgattat
     1621 gtatagttat catgacccgt attcactcca agaactccat gctcagtcac tattacatgt
     1681 tttaccatta aacaaacaga tagatcaaaa agaacaaaaa agacaagaag aaagcttgtc
     1741 agaaaaaaaa caacaaatgg ctcatgcagt ttctccaaca gggaaagtaa aacaaacaag
     1801 aagcctagag ttagcaagaa tatctagaaa aatctcagaa tctaataaaa cgataaggca
     1861 agaagctcat ttggccccac aagtttctgg attggaaaat gaaattttag agcctaaaat
     1921 aaaacaacaa tcagaaaaag aatttgttgc taaacagtcg gaagatcaga aggaatcaga
     1981 tgaccagaag gaaacgaaga aaaaacgctt gtcaactatt gattccaccg atgagcatct
     2041 gcagccgcaa gtaccacttc ctagtccggg aacacccgca cctggtggag gtattccttc
     2101 ttctgcttta ggagatttca tgcgaggtat ggcaggaaca tgggtatacg ctaatcgaaa
     2161 tgaatttgac gcagatgact ggttctgatt tatatctcta tctgttatag tcaaataagt
     2221 agctgagtca tatgttattt ggcataatgg agacgaggag ggatagaaaa atgttagata
     2281 aaacgttacc ttacgcagaa atctggatga caagaccact tcaacaaaat gttccaatca
     2341 cgcctttagc ggaaggtttt gaatttcgat tttatcaaaa agaagatgaa tctgcttggg
     2401 cggagattga gacttccgtt ggggaatttc aaacgacaga tgaagcactt gagtattttc
     2461 aacaaaactt tgctccttat cctgaagaac ttgaaagaag gatgtttttt gtagagacaa
     2521 aaaaaggaga aaaagtagca acctgtaccg cttggcgaaa aaaatcaaga gagcaagtgt
     2581 atccggtttt tcattggtta gcagtgaaac cagattacca aggaaaaggg ctcgcaaaag
     2641 cattgactgc cagagtcttg caagaatttc ctaccctcca tacggacgga ccaatctatc
     2701 ttcacacaca gacatggagc catcaggcga ttgctcttta taaaaaaatg ggttttactt
     2761 ttattgctga aaatctagac ggcacaccta atccagatta tgaaaaagtt atgcaggttt
     2821 taagtaaatt agataaataa cgaaatggtg aaataagtcc tcagtattgg gataaaaaac
     2881 gaaatagtca ttcctcatat cttacttttt tgagttaata ctatcggata actaatggca
     2941 tgccatacat tcaaaaaaga aggggcagtg acaaaagtat tgtcactgcc ccttctcttg
     3001 ttttttagca tgttttatcg tttgattttt ttaggaataa aattcttgat gaacgtaaat
     3061 ttttggtaat cgtttcgact atagaggtaa ccaaatacac tgaaaacaac tgctccaata
     3121 aaattaacaa ataaatcctt catagtatcc atgattccca cgtctaagta accattttgg
     3181 atgattgttt tatctgtttt accatctgca ttttgacttt caatgattgt ccgagtgata
     3241 tctggaacat gatagacagt gtttccgtct ttacttaact ccacactatt gatttgctgg
     3301 atgatcgtat ctttttgcat atcgaattgc agtaatttat ctgctgaaaa ttccacaaac
     3361 tcccacataa caccaactgt catcgaaaaa cagaaggaaa caactgctag taataaagga
     3421 gtcaagttca tggtttttgt atttttattg agcaaataaa caagggaaaa gccgacaccc
     3481 ccagctaaaa atccgctgaa tgcatgtaga aaagtatccc acattgaaaa atagccataa
     3541 aaatcactta actctcctaa aactgttgaa ctaaaaataa aagataacaa aatgatttct
     3601 agcatatttg gaatctgtat ctttaatatc ttttccagaa aataagggat ttcaaataaa
     3661 attaatgtca gtaataaaag cattgtattt ccccagtgtt ggttccagac ctgatccact
     3721 agcataataa cgacgatgat acgtaataaa atatagattc cgtttgtgat tctacctgca
     3781 gtttttgtac tgttcgtaaa aaaggttttc aaatgattca taaaataact ccttcatgta
     3841 tatccttaat tatactaaga agcgtgtgtt taccaaagct atatgactta aagaaaaaca
     3901 gagttgatat tgtgaaaaaa atcacttaat atagtataag gagaaaaggg ggatacaaat
     3961 gaaaaaatta gttgctggtt tattaacagc cgttgcactt cttgctatga gcgcatgcgg
     4021 cgcagatcag aaaaatacag cgcagacaag ttctagtacg aaggaatctg ccgaagtatc
     4081 atcaggcgct tctgaacaag tatatacaga tccaaaagaa atgaaagaat cttatgatgt
     4141 cgtgattatt ggagctggtg gtgcagggat ggcagcagct atcgaagcaa aagatgctgg
     4201 gttgaatcca gtaattttgg aaaagatgcc tgttgccgga ggaaacacat ctaaatcatc
     4261 aagcggtatg aatgcttcag aaacaaaatt tcaaaaagaa gaaggaatca aagactcgaa
     4321 tgatgccttc tatgaagaaa cgctaaaagg tggtggagga acgaatgata aagaaatgct
     4381 tcgatacttt gtcgatcact ctgctgatgc ggtcgattgg ctagatcaaa acggtatcaa
     4441 actggacaac ttaacgatca ctggagggat gagtgaaaag aggacacatc gtccgtctga
     4501 tggctcggct attggtggat acttagtgga ggggttattg aataacgtcc atgagcggga
     4561 aatccctatt tttgtcaacg cagatgtgac agacattaag aaaaatgggg aaacggtcaa
     4621 tgaagtgact gtacatgttc aaggagaaga accaaaagag gtcaccggaa aagcaatcgt
     4681 agttaccaca ggcggttttg gtgccagcaa agaatttatt gaaaaatatc gtcccgattt
     4741 agctgattat attacgacaa atcaagaagg atcaactgga gatggtatca ccatgattga
     4801 aaaattaggt ggacaaaccg ttgatatgga taaaatacaa attcatccaa ctgtgcagca
     4861 ggatgagggg gtcttgattg gtgaggttgt tcgcggagaa ggagcaatct tggtcgatca
     4921 agaggggaaa agatttgtca atgaaatgga tacaagggat aaagtatctg cagcaattac
     4981 agcattgcct gaaaaatcag cttatttgat ttttgaccaa ggcgtgcgtg atcgtgccac
     5041 agctattgaa ttttatgacc aaaaaggata tgtcacaact ggaaagacaa tggaagaatt
     5101 agcagaaaaa atcgatttac caaaagaaac actagcaaaa acagttgatg gatggaatac
     5161 ggatgttgcg gagaaaaaag atgctcaatt caaccgtgcc acagccatgg aacatgactt
     5221 atcgtctccg aattattatg cgatcaaaat cgcaccagga atccattaca cgatgggcgg
     5281 tgtgaaaatc aatacgaaca ctgaagtatt agataaagaa aaccagccaa ttagggggtt
     5341 atatgctgct ggtgaagtca caggtggctt acatggaaac aaccggatcg gtggaaattc
     5401 agttgcggag atcattattt ttgggcgaca agcgggcata caagcagctc gatttgcttc
     5461 ggaaaatgaa tgaaatagaa cgtaaataaa aagaggttgt gaaatcagcg ttttgagcta
     5521 ataccgaggg tcagctgatt gaacactgtt tagtgaagag gttgtgaaaa agctgccctg
     5581 catcaagtaa taagaacagc caagtaaaaa cagcttctta tgtttttaac ttggttggac
     5641 ttattaccgc agatgcaagc ttttgaacac cgtttattcg gaaaaagagc gtgggacaaa
     5701 agtaaagaac acttttgtct cacgctctaa acacgtataa acggcggaag cagaagaaat
     5761 tccttcggaa ataagctgaa attcacaaaa atttgaaaag caattttcgt gaatttcttc
     5821 ttatttcttg gagttaaacg cttctgtccc agcctctttt ttgatcagca tgattttatt
     5881 cagttatctt caaaatgatt tttccgatgt ttttgtttgc ttccatatat tcatgcgctt
     5941 gtttgactgc gctgaattgg aatgtacggt caatgattgg ccgaatcttg ccattttcta
     6001 gaaaaggccc agctttttgc ataaaagttt gtgcgagctc tgctttatac tgatcacttc
     6061 taggtgtcaa taatgtacca agcagttgga cacgtttccc aatcaataca ccaaggtcta
     6121 tctcaggcac gatggtccca cctagcatac caatcaagac ccaacgtcca tcaacggcta
     6181 tagaagctaa attcttttcc cagtaagaag cgccgatgaa atccaaaata acatcgactc
     6241 cccgaccttc agtgattttt cgtacctctt cttgaaagtc ttgtttttta tagttgatca
     6301 attcatctgc accaagttct ttacaaaagg cgagtttttc ttctgatcct gcagtaacga
     6361 tgattttcgc gtgagaaagc tgtctagcta attggatcgc tgctgttccc acaccacttg
     6421 ctcctgcatg gatcaagaca gtttcgcctt cttgtaattt tccaagccag taaagtgttt
     6481 gataagcagt caaaaaaact tctgagattc cagcagcaga aacatagtct agcgtatcag
     6541 ggagaaggat tgcacgatcg gcaggcatca cagcatattc ggcataacca cctagattga
     6601 ctaatccgta tacttttgtc ccaggagtaa aagtggagat atcactttta ttctcaacta
     6661 cctctccagc tacttcgact cccaaaatag ggtagggagg tttcaaagag actgattctc
     6721 ttttcataat atctgttcga ttgacagctg ctgtatggac tttgatcagt aattcgcctt
     6781 gttttggttt aggaacaggc acttcaattt gttctaatac atctacagaa ccaggatgct
     6841 tgattgaaat ggcacgcatg gatctattcc tcctttgccc attcttggaa tgctgtattc
     6901 aactctttgc taccttcaga aagtactaga gtcgtacgag aaatatccgt ttcttccact
     6961 agagcggcaa gtacagcagc aactgtagag cgagtgatcg tttttgatgc cttctcatta
     7021 ggcttagtta attgaatgga agtaacttct tcatcgttag tcaatgtgac tggttgaaga
     7081 atgacaaaat ctaagttagt tcgtttcagc cattcatctg cataatgttt agtaatataa
     7141 taatctgtca tggactcagg ccattttgct ggttcatctg cataaacagc actgaccatc
     7201 agatagcgag agacatgcgc attttcagct gcgatcactg ttttgattgc gccatccaag
     7261 tctacttgca gaagattctt tccttgagag ccggcagcaa aaatcaatac atcgatgcct
     7321 ttgaatgctt cggtcatttt ttccacggac caagttaaat caaaagagac atacgaaaca
     7381 ttctcttttt ttactaatgt ttgggaagca acatctctca cccctgcaaa aatttcatgg
     7441 gaacttggag ctaaatcttt tatcaggtgt cggccaattt gtccgtttgc acctacaaca
     7501 aaaattttca tttggatcat cctttcgacg tcaaatcttt ccattcaata gtaacaccaa
     7561 aagagaaaaa aatcagagat cggctacgat gactatcagt tagtaagaga agggtgcttg
     7621 aagtatctta acaagacatt gcatactgct gcagtcgcaa gacctgcgac gattccttca
     7681 ggtataccac ttgtaaaaat aaccgctaga atagctgaat aaacagcatt taaatttgtt
     7741 ccgatgactt tggcataatc ttgctggaat aaaaagtaga tcatattcat cactaaaatc
     7801 gtattggtca atgaaccgga aagtccagct aagaataaag aaacggaatc tgctttttgt
     7861 ttcattagtt tgcgcagacc tttataaacg aaataaggaa ccaccccaat caagatgcga
     7921 ggaataaacg cgatcagcaa ggctttccag cttccatgtt ctgttccgat gactgggata
     7981 aacggagaaa agacaaaaga caaaggtgtt tggataactg tgctacggat catgctgatc
     8041 agtccaaatg taccgccaag aaaagcacct agtcttggtc ctaaaacgat cgaagcaatg
     8101 attacaggaa tatgcatggt tgttgcattg attggaccga tagggataaa acctaaaggc
     8161 gtaacagcta ataaaatcaa aatagctaaa aacatcgcag ttaatgtaaa gttttttgta
     8221 tttttcataa gtactccttt tttctaagca tttctttcat taagagtcag catgacctgt
     8281 tcgatgatcg tctgaatatc agctaaggca ccttttccaa aatctccaca agctaaaaga
     8341 gattctcttg gttcaatttc ctgatagccg acagatctca atgtttccag attacgctga
     8401 gtgatcgggt tttgatacat attcgtgttc atggcagggg caatcaattt tggtgttccc
     8461 actggaagcg ccattgcgac tgttgaaaga agatcgtctg caatgccgtt tgctagcttg
     8521 ccgataatgt ttgctgtagc aggcgcgatg agaaacaagt cagcttgttt tgctagttcg
     8581 atatgattga ttacctgcgg atttgattct tgcatgacgt ctgtatggac tgcgcgcttc
     8641 gacagagatt gcaaggttaa tggcgtgata aaatgtgtgc tgcttttaga cataatcaca
     8701 tctacctggg cacctaattt gacgaattga cttgtaagat cagcgctttt gtaagcagaa
     8761 atacttccag taacgcctag taagatcttt ttcattttga ttcctccagt ctaattttct
     8821 cagtaatcaa tgcagcaatc tcagatttac tttgagcttc ttccactgtt ccatctttag
     8881 ataagagata gccgtggtgt gtctcgccat ggatttccgt caaatcattt gcgaaaataa
     8941 aatcagtttt gttatttatc agactatttt ttgcgactgc cagaagagat tcctttggta
     9001 catctactaa tagtttgaaa ccaatgattt ttgcttttgg ctgccattca cgtagataag
     9061 aaatgatttt tggtgttttc ttcaaaacga gcacaagatg ctcggtgtta gaagagatct
     9121 ttgaaacagt ggtatctgtc tgttcatttt ccgcaaacca attgtccagt tcttcttgcg
     9181 ttgaagaagt gggtaggttt tttgccagct gttcttctga aaaactaaaa gcaggagtaa
     9241 aatcactgac tgccatactg tggataatcg catcgtagtg ttcttttttc gttagttctt
     9301 ctaattgtaa aaataagtcc agtgcagatt cgatcgtata aatcgttata ttggagtgtc
     9361 ttttcggttt cagtgcttgt ttcgtagtca cgtaatcgat tgtggtatcc ggattggcag
     9421 ctaaataatc agctaatgct tgccctaatc gtcctgtcga atgatttgta atagcacgta
     9481 cttgatcgat tttttcagtc gttcctccag ctgtgattag aatcttcatt tatggtcacc
     9541 tacatttact agtgttcttc cttggtgtgt tccagcaagt agtttagatg ctgtttcagg
     9601 caattgatcc agagtaatct cttggacgac cagttcatct gcaatctgca aatccgccat
     9661 ctgttgccat aaagaaagac gttgatctat tggtacattg acagaatcaa ttccaatcaa
     9721 ctggatgttt cgcagaataa aaggcaagac agtcgcgttt agtttgattc ccccagcatt
     9781 gccacataaa gctactgcac cgttataaga gatcaatgga agaagactgc ttagctgctc
     9841 gccacctact gtgtcgatga catagtcgat ttgctgtttt cctaaagctt ttgttgtctc
     9901 tggcagaaat tcatcaggtc gtacgacttg caaagcacct agacttttta gccatgtgac
     9961 agactcttct tttctggaaa aggcaatgat tgactggaat cctagtttat gtaacagggc
    10021 tatcgctgta ctgcctacac cgccagaagc accagtaacg acaacttttt tatcggaggt
    10081 cataccttga cggattaaag cgttcacaca caacatagca gtaaaccctg ctgtaccaag
    10141 gatcatcgct tgacgcagcg tcatattttt tggtagagga actaaccagt ctcctggtac
    10201 tttttgatat tggctataac cgcctggatg ggtgacgcca agcccaaaac cagtagcaag
    10261 tacaagatct tcttttgaaa aacgtggatt gttactttgg acgacagttc cggataaatc
    10321 aatccctgga gtcatcggat atgttcgtat cacctgacca gactcgctca ttgccaatgc
    10381 atctttataa ttgacatcag aataggcaat ttttaccaag acttcatttt cagccaactc
    10441 agtaaatggc agatcactga ttgctgtatg gaaaacaggt tcctttatta cctgaaaaga
    10501 tcgaaaagcg ttcattcgtg agacactcct tttaaaattt tcttttatca taccataaag
    10561 cctactcttt tattcttgtt cttccgtaaa tttagacaaa aattaggtga aaaataaaga
    10621 atcaaacaac tagaatttat tccaaaagat aaagaaatat tcagcaaaaa aaattttaca
    10681 aagggttgcc tttttcaata aatttcgtat actaatacga gtggtagtaa acaggaagtt
    10741 tactattaga aaacttcgat tcggaggaaa ttatggaaat tggtgagaag ttaagaaatt
    10801 tgcgcatcca aaagaatttg acacaagaag aactaggcga acgcacagat ttgtcaaaag
    10861 ggtatatttc ccagttggaa cgagatttaa gttcaccatc aatggaaaca ttcttttcta
    10921 ttcttgaagt cttaggtgtg actccagaag aattttttca tcaagaagca gcgaatctgc
    10981 aagtggttta ttcaaaagaa gatcatacgg tttatttcga tgaagaaaac gggtatgaat
    11041 tggaatggct cgtggctgat tcgaatgaaa aagaaatgga gcctgtcttg ctgacttttg
    11101 aaggtgcagg agaatataag acgtttgaac cttcattatc tgaaacgttt atttttgtat
    11161 tagaagggga attagagtta agcttaggag agacggtcta tgtggcaaaa gcaggacagt
    11221 cgatctatta tcaagcgaca aaacaacatc aattaaggaa tcatctcaaa aaacgaacaa
    11281 aagtattgat tgttgcaacg caatcatatt tgtaatatag ggggtaaaaa cgtggggaaa
    11341 ccaatcattt catttgatca agtagtgaaa cgctatgatg atgaaacagt attaaaaaaa
    11401 gtaagttttg agatcgaaca aggaaaattt tatacactgc ttggtccttc cggctgtggt
    11461 aaaacaacga tcctacgtat tatagctgga ttcaccgaag ctacagaagg ggacatctat
    11521 tttgaaggga aacggttgaa tgatttgcca gctaataagc gacaagtaaa tacagtcttc
    11581 caagattatg cactttttcc tcatatgaat gtttttgata acgttgcttt tggattgaag
    11641 atcaagaaaa tgtccaaatc agatatcgaa aagaaggtaa aagatgcttt gcgtatggtc
    11701 cagcttcctg gatacgaaaa acgggagatt agtgaaatgt ctggtggtca aagacagcgt
    11761 gtagcgattg cacgagcaat tgtaaatgaa ccgaaagtat tgctattaga tgaaccgtta
    11821 tcggcgcttg atttgaaact acgtacagat atgcagtatg aactgcggga tctgcaacaa
    11881 cgattaggaa tcacgtttat ttttgttact catgaccaag aagaagcgtt ggcaatgagt
    11941 gatgagattt ttgtcatgaa taaagggaaa atcgtccaaa gtggtacacc cgtagatatt
    12001 tatgatgagc caatcaatca tttcgttgct gattttgttg gggagagcaa tatcgtggac
    12061 ggtgtaatga tcgaagataa tctagttgag tttgttggta agcaatttga atgtgtcgat
    12121 ggcgggatgc gtctaaatga accagttgaa gttgttttac gcccagagga tttgacgatc
    12181 acgacaccag ataaaggaaa actagtcgta acggtcgata ctcagctgtt tcgtggtgtc
    12241 cattatgaaa tcatctgtta tgatgaacag caaaatgaat ggatggtcca ttcaacgaaa
    12301 aaagctaaag aaggaagcaa agtaggattg gcctttgagc cagaagatat ccatgtcatg
    12361 cgtttcaacg agtctgaaga agaattcgat gcacgtcttg acagctacga agaatagggg
    12421 ggacaagaat gaaaacaatg cgcaggatct attccgtccc ttatattttg tggctcggtt
    12481 tattcgtgat cgcgcctgta ttaatgatca tttatcaatc tttttttgat atgaatgggc
    12541 aattcacgct gaataattat ctatcttatt ttacgtcggg gacatcgctt tctatgacga
    12601 tcaattctgt atggtatgca tttttgatca cgtttttcac gctgatcatc agttacccga
    12661 ctgcttattt tttaacaaaa ctgaagcaca agcaattatg gctgatgctg attattttgc
    12721 ctacatgggt gaatctgctg cttaaagctt acgcatttat tgggattttc agtgtgcacg
    12781 gcagcgtgaa tcatttcttt gattttatcg ggatcgggcc gaaacagata ttattcacag
    12841 acttcagttt tctgttcgtg gcaacgtata tcgagattcc atttatgatt atgccaattt
    12901 tcaatgcatt ggaagagatg aatccttctt tgatcagtgc tagtagagat ttaggcgcga
    12961 atagctttga gaccttccga cgagtgattt ttccgttatc attaaatggt gtgaaaagtg
    13021 gtgtccaagc agtatttatt ccttctcttt ctttattcat gctgactcgt ctgattggcg
    13081 gaaatcgggt gatcacttta ggaacggcta tcgaacagca tttcttggtt acacaaaact
    13141 gggggatggg ttctacaatc ggtgtgattt taatcattgc tatgttcatc gtgatgctcc
    13201 tgacaggcga gaagaaaaaa ggaggtcgct cataaatgaa aaaatttaaa tggtcgtatc
    13261 tttatttagg gatcgtcttt ttattcttat atgcaccgat cttctatctg attttttatt
    13321 ccttcaatga aggaggaacg atgaacagct ttacaggatt tacttgggaa aattatgaag
    13381 ctgtttttga agatacacgt ttgatcatta tcgtattgaa tacgttcatg ctggctttct
    13441 tatctgcatt gatcgccacg atcatcggga catttggggc tatgggaatc tattacacga
    13501 aaaaacgacg tgcacggaca gcgttgttga gcatgaataa catcttatta gtctctcctg
    13561 atgttattat cggtgctagt ttcttgattt tctttacact actaggattc ggattaggtt
    13621 ttcagagtgt attgctttcc catattgctt tcagcattcc aatcgtcgtc ttgatggtct
    13681 tgccaaaatt gcaggaaatg aacgattcaa tggtcgatgc ggctagagac cttggtgcta
    13741 ataatttcca agtagtcaaa aatatcattt tgccttatct ttctccagga atcatagctg
    13801 gatattttat ggcatttacc tattctcttg atgattttgc ggttacattt ttcgtcacag
    13861 gaaatggctt tacgacgctc tcagtagaga tctattctcg tgcaagacaa ggaatcagtt
    13921 tagaaatcaa tgcgctaagt gcgttagttt tcttgttctc catgatattg gtcattggat
    13981 actactttat tagtaaagaa aaccgtccaa aacggctgaa gaaaaacaga agagtacgcg
    14041 agggggtggc acggctgcga tgaaaaaatt acaatctctg tttttaggta ttctcatcat
    14101 tattgcggtg ctgctgcttg gtgttcgtca acttgaaaaa acaagtggca tgagtggagc
    14161 aaaggtactt accatctata actggggaga ttatatcgat cctagtttga tcacgaaatt
    14221 tgaaaaagaa tatggataca aagtgaatta tgaaacattt gattctaatg aagcgatgtt
    14281 cacaaaaatc cagcaaggtg ggacaaacta cgacatcgct ataccaagtg aatatatgat
    14341 ccaaaaaatg atcaaagaaa aactagtact tccattagat cattcgaaga tcaaaggatt
    14401 aaataatatt gatccacgat ttttagactt ggattttgat ccagataatc agtattcgat
    14461 tccatatttt tggggaactt tagggatcgt atataacgat aaattctttg atgcaaatca
    14521 aattaagcat tgggatgatt tgtggaagcc ggaattaaaa gacagtttga tgctgattga
    14581 cggtgcacga gaagttatgg ggctttcttt gaatagttta ggctattctt tgaatagtaa
    14641 gaatatggca cagctgacag aagcagcaaa taaattgaat catttaacac caaatgtcaa
    14701 agcaatcgtg gcagatgaga tcaaaatgta tatgatcaat gaagaagcct ccgtggcagt
    14761 gacattttct ggcgaagctg ccgatatgat gtgggaaaat gaacatttac attatgtgat
    14821 cccaccagaa ggatccaatc tttggtttga taatatcgtt atgccaaaaa ccgtgaaaaa
    14881 taaagaaggt gcctatgatt tcatcaactt tatgctagaa ccggaaaatg cggcacaaaa
    14941 tgcagagtat attggttatt ctacaccaaa tgaagaagca aaagcgttat tgccaaaaga
    15001 catttcgtcg gacgaacaat tctatccatc tgatgacacg atctcccatc ttgaagtcta
    15061 caaagatcta ggatcgaaat acctaggcat ctataatgac ctattcttag aattcaagat
    15121 gtataggaga taatggcaaa actcagtaag aatggggact tatcaacgtt ctaaagagct
    15181 aaaaatcgtc ctttttctat ctgagttttc caatcttcta ccaaaagcgg atgaaacgcc
    15241 gctttttttg tgttatttta ttttttctac cacgtttttc taccagagaa aagtggacaa
    15301 aaccattgta aaatagggcc cgagacgggc tagtaaatct aagattgaaa aagttacaga
    15361 aggataaagt aattggaaac tatgcaaata tacttaaccc ttctgtaatc tattaataaa
    15421 actaatatat gaatccagtt caatatttgt agctatgaaa aaacataaaa atcatattag
    15481 ataaaaacta aattggtaga taaattattt tgtcagatat ctgaggcaaa tattcgacta
    15541 tttgtgtttc aaagtaaaaa ttggaataca aatatagaat agacatattg cagccgatag
    15601 tttattatgc ttttagacac attaaactgg aggaaaaaat gaaaaagagc gctaaagatc
    15661 gagtttttga tgagctgata cgactaacag ggaatatgtc ttctgtcact gccgagaaaa
    15721 tttcgaagaa tctaggtatc tctcgtcaga atgccagcca ttatttgacg agattagtgg
    15781 aagataaaaa ggtcgagaaa ttaccgggaa agcctgtttt gtggaagcct ttagatgaat
    15841 atgcggttgt agataatacg gagcaaataa acgaagcctt tcattctgta gttggtcatg
    15901 acggaagtct gagagaggtc attcaaaaat gtatagccgc tgtcaaatat ccgcctaacg
    15961 gactcagtgt attaataaat ggagccacgg gtgttggaaa aagtttttta gccacaaaaa
    16021 tttttgaata cgctatccat gagcaaatta ttgaaaaaga tgcaccattt gctatcttaa
    16081 actgtgccga ttatgcggac aatcccgaac tgctttcggc aacactgttt ggatacaaga
    16141 agggggcctt tacgggggct gaaaaggata ccgatggttt attggcaacg gccaacaacg
    16201 gctatctttt tctcgatgag gtccatcgcc tatcgaaaga aaatcaagaa aagctctttc
    16261 tatttattga tactggaaac tatcgtccag tcggtgaaaa tgtaaattgg cattctgcta
    16321 aggttcgttt tattttcgcc acgacggaaa aaggtgaaaa ttatttatta gacacctttg
    16381 atcggcgtat tcaaattagt gttatgttgc caacttttga agatcgcccc attcgagaac
    16441 gattagagct gattcaatta ttttttcaaa atgaagcgga tgttttacaa aaagatatta
    16501 ttgtttcaag agaagccttg tctttaatgt tatctcatcc ctttagtggg aatattggta
    16561 agctgaagaa tatcattaaa atcagttgtg cggatgtgta tagtcgaaca caagaagaac
    16621 cattaacgat tgggaaaaat gagatcttga ttcagttaaa tatgctggaa tccgaagtag
    16681 aaagcttgcc gattgctagt ttattgataa agaaaaatca aaagataaaa acggatcgaa
    16741 tcaaaccgtt tgattatgat tcgattttgg aaaaaattac tcagctgata aatgaaggta
    16801 atttcaatga aaatttttct tcgataaaga atgaagtaca gcatttggtg catcacattg
    16861 ataaggattc ttttttatca atgagtagaa aattgatgat tcagatgttt agaaaggtct
    16921 gggtatccgt tttaggaaaa acgtacggat tgacgacgag tggacctgtt gcagaaatta
    16981 ttagcagatc ctatgtcatt tccccaagct ataagacaga cttaaccggg ataaatcgta
    17041 cattagctgc aaatctacct cggacgactt acttatgtaa tcagttcttg gagcatttac
    17101 caccgctctc agaaaaggac gagaactttc taagactgct tattgtcgtg actttatctg
    17161 attatgtaga tgaatccatc gagctgaagg gcctgttgct tgctcacgga gatagtacgg
    17221 ctagcagcat tcaagcagta gtgaatcaat tatgcggcaa ttatgttttt gaagcgttgg
    17281 atatgccagt agagagtaat gtgtcagaga ttgtcgaatc gacaaggaat ttcgtgaaac
    17341 ggcaagtgaa tacagatagt ctagtgctga tcgtagatat gggctcttta aatcaaattt
    17401 acacacagat caagaacgat ttaaaagggg agttacttct tctgaataat ttgacgactt
    17461 ccattgcact tgatattggc ttgaagatga cgatgaatgc gccatttaaa cagattgcgg
    17521 aggatgcagc agacaagtat tcaatcaatg ttcaatactt tgaaggcttt tctcaaagta
    17581 gcaacattat catttcctgt atgtcgggtt tggggatttc ggacaaattg aaagatgttt
    17641 ttcgtcatca tttatcggat gaatccatcg aagttttcac gaaggactac agagaactac
    17701 gtgaattaat cgaccaaaat gatgaaactt attttgacaa gacaaaattg gtcatcacga
    17761 cttcggatct tccacgttcc ttttcaattc ctaatattaa tgtttatgac atcttagacg
    17821 gtgatggagc agccaatttg gagcatattt tgagtcctga aatgaagccg gcagaattta
    17881 accgcttgat tcaggagcta gtaaaattct tttcgttgga aggaattgcg gaccgtttga
    17941 actttctgaa cccaacgatc gtgatgagtg aagtggaaac agtcatttct aaatatgaaa
    18001 attactatca tttcacttta aatggccgtg taaaactaaa tctctatatg catacggcat
    18061 tgatgatgga acgtttattc ttggctcgga cagaaaaatc tgaagaaccg aattatgatt
    18121 tgaaaccaga tgaagatgaa ttttattatg tttctagaag tatttttcaa tcaatggaga
    18181 tgaaatataa ttttaaagtc aatgcttatg agctttcgct gctctatgaa ttattaggac
    18241 cgtatattca aaaataaagt gtctaaaaaa gtgtctaatc tgtttttttt aggttagaca
    18301 ctttttttaa tgtgaattaa cgtgattaaa ccaatgtttt taaaagttgg cacaggaatt
    18361 gcttatataa aagtgaacag aaagaaagga attaaccata tgaagaaaat tctaatagcg
    18421 actcacggtc acttagcttc aggctttctt agcagtattc aattactgac ggggaaggtt
    18481 caagagatta cggtgattaa tgcttatgtg gatgagtgtg attttgaaca ggagttgaat
    18541 gtatttgtaa accaggtgaa cgagcaggat cagatttttg tttttaccga tttatttggc
    18601 ggaagtgtca atcagaagat taccaaaacc tttttggaga aagagattgc tgtaactatt
    18661 ttgtctggct ttaatctgcc aattctgctc gaaatagtat tggcaaccaa ggaattaaca
    18721 aaggaggata ttcaggaact ggtaaataaa tgccaacagg aattaaagat gaccattctt
    18781 gatgaggtac atgtagtaaa tgaagaggaa gatttttttt aggaggaaga aaaatgatta
    18841 aagcagtgcg aattgatgaa cgattaattc atggacaagt agcgatgact tggacaaaga
    18901 gtctaaattt aaccgggtta gtagtagcaa gcgatgaggc ggctaccaac gatattcaaa
    18961 agatgacatt gaagatggct gcaccatcga atgtcaaagt cattattaaa acagttgatg
    19021 acgcgattca tttgttaaaa gatccgcggg cgaaggacat gcgcttgatg gttttagtcc
    19081 ctaatgtcaa agatgcggta aaagttgcga aagctttccc aacggaaatc gaattaatga
    19141 acgtggggaa tgctgggaaa atgacaccgc catcggaaga taagaaaacc ttatcaaagg
    19201 aagtgatgtt aacgacgtca gaaattcaag acttaaaaga attagttgaa ctttatccag
    19261 ataccgtttt tcagcctaca ccggcgatgg aaaaacgttc agctatctct attttgagtg
    19321 atttttagaa aaaggaagga gaaaaattat gtatgatgca tttgtagtcg ctttggccgt
    19381 ttttattgga gttgctggac atgaaatgtt cgggatgtcc atgctgagtc gtccgattgt
    19441 tgttgctccg ttagcaggag cgttgatggg ggatttgcag acggggttga ttgtcggggc
    19501 gtcactagaa gccatcttta tgggtgtcgt caatatcgga atctctagta cggcggagcc
    19561 ttctttggct gctgggctgg ctacagcgtt cgcaatcaaa atgggaggaa acgtggatgc
    19621 gattattccc attgtctttc cactagcagt attaggatta cagttaatga atatgatttt
    19681 ttcttttgta tgtggaccga tggctgctaa atttttcaaa tttgcaaaat ctggtgatca
    19741 aaaaggaatc atcaatctgc acttctttat gtggtttgtc cactatgcgc tttatgctct
    19801 tattccattc tttgccgttt tatttggagc agatgttgtc aaaagcgtct tggataatat
    19861 tccagaggtc atcatgaacg gattaacagt cgctggaaat ctgctgccgg cagttgggat
    19921 ggcgatgctg ttacggatgc tttggagtaa aaatattgct gtgtggtttt tctttggggc
    19981 cgttgttatg gcgtatctcg agttaccatt gattgcaatt gccgcagttg gaacgattat
    20041 tgcaattgtt atagcccaaa gagatctaca actaaaaaat atatctgttc atgcagttca
    20101 aagcgatgct gctgtaaatg caacgcttga ccaagaagag gaggatttct ttcaatgaaa
    20161 ttagctgatg gaatttctaa agatgagaaa aaattggttc ggaagatgtt ttggcgctcg
    20221 atgactatgt atgcttcggt gaatccggtg acgatgggcg gcggtggttt cgcctattct
    20281 atgatgccct tcattaatca tttctacaaa agtgaagaag atcgagctgc ggcgctggaa
    20341 cgtcatacac agtatttttc gacgaccatt ccttgcgcgt cctttgtcat ggggattgcg
    20401 gcatcgatgg agaaggaaaa ttctgaaaaa gctggttttg acgtggaaag tatcaacgcg
    20461 atcaaaacga gtttgatggg accattggct ggaattggcg attcattgtt ctggggagtt
    20521 tggcgtgtcg ttaccgcggg actagcgatc aacttagcca atgccggaaa tattttggca
    20581 ccaattatct ttttagttat gttcaatata cctaattact tgatgcgata ctatggcggc
    20641 gttttaggct attcattagg ctctaaatat attgaaaagc tgtatgcgaa cgggttaatt
    20701 gaaattctga caaaagctgc tagtatcttg ggtctgatca tggtaggtgc aatgacgatc
    20761 agtatggtca aattcaatac cgttttgagc ttttcaatga agggcgaaga actaatgaat
    20821 gtccaagaaa tcctcgatag tatttttaaa ggaattgtac cgttattggt aacattgagc
    20881 tgtttcaaat tactagataa aaaagtcggc gtcatcaaaa ttatttttgg aatggtcgtc
    20941 ttgggaattt tattgtcatt acttggaatc gcatagtagg aagctgcagc tactaggctg
    21001 cagttttttt agcaaggagg gaaaagatga ctattaatga agtaatttat tcacccaaac
    21061 acccattttt taatgctgag ttaaacgacg gaaatgtgac tacttcaaac gaatacagct
    21121 actatcggaa tattcctgat gtaaagggat tgaccttcga tgtaagtaat caaaagacat
    21181 tagcaaacct tgatgtttgc gggacgattc aaacgttgac cttttatcga gaaaatttac
    21241 taactgagga aaaaccaggt gtttgggtca ataaacagct ctctcaaacc aattcgttat
    21301 ccttaaaggt tgaagtaaac gggattgtct accatttgtc agaatcggat catcgtattg
    21361 aagtcgatgt gattcaagac tgtttgccta gatatattca tcattattcg acgtttaagg
    21421 tcattgtagt atcctttgct cccatactgg ctaataagcg tttaagtatg cttgttcaac
    21481 aggtttacat tgtgaatgaa acacacgaaa gccttcaagt ttcagtgaaa gatgtgcctt
    21541 cgtatcaaga gaagtattcg gatcagcaaa atgtgttgat ttctcaaaaa ggaaataagg
    21601 acacgatcgc acaacaggaa gtggcaagct tcgccattgc ttttgtggac ccaaatgctt
    21661 ttgaagaagt aatgactttt caggaatctg atatcgaaaa atggctgaag gaaacattga
    21721 actattttgc tcagctattt gggcagctgt caatgccaaa cgatagagtt gtccatctct
    21781 atcgtcgtgc actttatcaa agcttttcta gtttcggtat gaatcgacag ggacaggtcg
    21841 tcggcagcaa ttggggcagc tatcctgcta ctaatcgaat ttggaataag gatatgtatt
    21901 acgcatcatt gccattcacc atgtttgaac cggagttatg tcaaaaaacg gttctatggt
    21961 ttgatcaata tggcgttaag tttcctggtt caaaatttac aggaggtatc aatcattctc
    22021 tgagtaattc gttatccagt gcgttactgg ctagtcttta ttttgaacat actggggata
    22081 caaaattctt tgagcggtgt ccgcaggttc tactgaatgc ttcaagaatt attgaggaca
    22141 tattagctca acgtcggtca ggggaaccaa tgctttttcc atctgtttgg ttatcagacg
    22201 cctttgcact tggaaagtat cataccggca gcaatctttg tctttggaag gcctgtattg
    22261 gacttgcaga aatttttgaa gccctagaac aactgagtct tagcaagcga tacaaaaaca
    22321 ttgcctgtaa actaaaggaa tcaatttgta aacaaatgac tatcgatggt ccttttggtg
    22381 agcaattttt ggagggaatc ggtgatgagg agaaaacaac ctattcagtg aagcactatc
    22441 aaaaaccgat tcttgagcaa gggttaatct ttttaagtga tgtgattgtg gatggaaaaa
    22501 ttaatttatt gatgcatgat ggagaagaat cagacactac gttgatgcct ttttatcaat
    22561 ttttagataa taaagatcat ttatatcaga acaccatggc cttttctgct agttctttca
    22621 atccaaccta tagcgaggaa atcaaaggca tcacttgggg attagaatcc ggcgccactt
    22681 ttcctggctt tatcactgtt ttaatgagcg agctgcacaa ccctcaagct ttcgtaaccc
    22741 gattagaaga actaatttgt ttggcagatt tagacggttc gtggtggtgg tggccgtatc
    22801 gcttagaggc tcaacaagga gaagtcgtgc gagactttgg ctgcggcaaa tgcggctggg
    22861 catcgggtct ttttgtcagc ctaatgatca gtcaatattt cggattgaaa ttcaaaaaag
    22921 gagtattgca aattgatcct gttgataatt taacgttttc ttggaagaat ttgaagttcg
    22981 gtcaagcctt catttcgatt gaatgtacgc aatccgagtt gagtatttca aatttgtcag
    23041 aaaagccatt gaaaataagt gatacgaaaa aaattcttca cgtagaaaag ggaaaaacga
    23101 ttcacattca aaggagaaaa agatgagtat actaaaaaat attacccaat ttaaaaaaat
    23161 ccagagtcga gcaatcactc ccgaaaatcc aacaggcgag aaaggtaaag ctgcgatgtt
    23221 gccaagcgag ctaggaccaa gccgcaaagg tcgtggcgca attccgctga cttctggtga
    23281 agaaactgtg attgcggata ttactggaac gggaataatt cgtcatatgt ggatgacgat
    23341 tcgtgatcga acagaatttg gcagttttgt tttacgtgat ttaatcttaa gaatttattg
    23401 ggatgggtcg gatagccctg ctgttgaaag tcctttaggg gatttctttt gtaatgggtt
    23461 tggagagcgt tgcgaggtga actcgttgcc gatagtggtt aatcctacag ggggctttaa
    23521 ttcctatttc gaaatgcctt tccaaacatc cgctaaaatt acgatcgaga atttacatcc
    23581 gaaggatatg tcctcatttt tttatacgat aaattacgct ttggtggacg aattggaaga
    23641 aaatagccta tattttcacg caaattggca tcgggaacgc attaccaaat tacaggagga
    23701 ttataagtta attgatcgat taaagggtca aggctattac gtgggtactt accttgcgct
    23761 tacctgccta gaacgttatt ggtggggcga aggcgagttt aaattctaca ttgatgggga
    23821 tagtgagtat ccgaccgtaa cctcaacagg ttcagaagat tattttggcg gggcgtgggc
    23881 ctttcatgag tacgatgagt tcggacgtcc acatgcgaaa aactattcaa cagccttttt
    23941 agggtatcct taccaatcaa atagagatgc cacaagagat cgttttgaaa cgggaaaatt
    24001 gaatgccgtt catgcctttg gtgacgacgc aatgccacga catggtcttt atcgttggca
    24061 tcttcaagac cccatctgtt ttaccgaaga tttatcggtc actttacaac aaatcggcaa
    24121 cgatgatgtt cgcttatttg agcggcaaga cgatgttgct agtgtcgctt actggtattc
    24181 aacagaagct tcaggaaata ctaaacgtca tttcactgat cgagaacgct tgccgcgtta
    24241 atgaagtgtt tttcacatat tcgaagggag gaacctatga tgatatatgg ggcaattgaa
    24301 gctggaggga ctaaatttgt ctgtgcagtc ggtactgagg aattaacgat tttggaaaga
    24361 gtcagttttc ccacaacaac tccagttgaa acaatgaagc aggtcattga tttttttaaa
    24421 ccttttaagg aacaattggc aggcatcggg gtcggctgct ttggtccaat tgatattcaa
    24481 tgcaaatcaa cgagtttcgg tcatatcaca tcaacaccaa aacttgcttg gcaaaatttc
    24541 aattttgtag gaacgctaaa gcaatatttc aatattccga ttgaatggac aacagatgtc
    24601 aatgcggctt gctatggtga atatgttgct ggttctggca aagggttagc aggtgttgca
    24661 tacttcactg tgggaacagg tatcggaggt ggtgccttgt tcaatgggac tttcgtggaa
    24721 gggttcagtc atccggaaat ggggcatatg ttgatcaagc gacatccaaa ggacaatttt
    24781 tcaggaaact gtcccttcca ccaagattgt ttagagggga tggcagcagg accagcgatt
    24841 gaaaaacggt tgggtgtaaa aggccagaat ttattggctg acgattcgtt ttggcagatt
    24901 gaagcctttt accttgccca atgtgcatac aatacaacac tgatgttttc gccagataga
    24961 attatttttg gcggtggtgt aatgaaacaa gaacacatga agaaaaaagt tcaggataaa
    25021 tttgttgagc ttataaatgg atatgttgaa ataccaccaa ttgatagcta cattattacg
    25081 cccgaattag gtgataatgc cggaataatc ggcggattag cattggccag aaaagcggta
    25141 agaaataagc aaccataatt aatgaatgtt tcattgatac tcatgtcaaa agtatttaac
    25201 cgagtaatca aatgatcaaa tgaagttgga taaagcgaaa atgaattcaa ataaaaaggc
    25261 tctaagtcaa atcgtgttgt tttcattaaa tcactgaaag gaaatgcttc tgcgtttcct
    25321 tttttctttt tgttgtgcgc tgtatacctt gaggatattc actggtggcg tacacttaaa
    25381 tagtaatata ttgtgttact ttatgtctgt ggtaatagtt atagttgctt accaatgtat
    25441 gttcattttt cgaaatgttg acttcataat ccttggctta atgtttgaat ttttttggtg
    25501 atagattttt tcagccgtat aaaataaatc gtaattctat tatagagagt tgtgattata
    25561 aaaagatagc aagaaaaaaa aagaaagttt ctatacattt taaatatttt attcctattt
    25621 cta
//
LOCUS       DACDPS010000033        25184 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.033, whole genome shotgun sequence.
ACCESSION   DACDPS010000033 DACDPS010000000
VERSION     DACDPS010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25184
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.033"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            237..1877
                     /locus_tag="IWJ07_001458"
     CDS             237..1877
                     /locus_tag="IWJ07_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAP9333045.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2031..3353
                     /locus_tag="IWJ07_001459"
     CDS             2031..3353
                     /locus_tag="IWJ07_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAP9333046.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3415..3846)
                     /locus_tag="IWJ07_001460"
     CDS             complement(3415..3846)
                     /locus_tag="IWJ07_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9333047.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(3910..4746)
                     /locus_tag="IWJ07_001461"
     CDS             complement(3910..4746)
                     /locus_tag="IWJ07_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAP9333048.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(4936..5238)
                     /locus_tag="IWJ07_001462"
     CDS             complement(4936..5238)
                     /locus_tag="IWJ07_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAP9333049.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(5264..5695)
                     /gene="ruvX"
                     /locus_tag="IWJ07_001463"
     CDS             complement(5264..5695)
                     /gene="ruvX"
                     /locus_tag="IWJ07_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAP9333050.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(5692..5958)
                     /locus_tag="IWJ07_001464"
     CDS             complement(5692..5958)
                     /locus_tag="IWJ07_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAP9333051.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(6212..6937)
                     /locus_tag="IWJ07_001465"
     CDS             complement(6212..6937)
                     /locus_tag="IWJ07_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAP9333052.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(7303..7373)
                     /locus_tag="IWJ07_001466"
     tRNA            complement(7303..7373)
                     /locus_tag="IWJ07_001466"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7339..7341),aa:Gly,seq:ccc)
     gene            7490..8242
                     /locus_tag="IWJ07_001467"
     CDS             7490..8242
                     /locus_tag="IWJ07_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAP9333053.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            8258..8737
                     /locus_tag="IWJ07_001468"
     CDS             8258..8737
                     /locus_tag="IWJ07_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAP9333054.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(8988..10568)
                     /locus_tag="IWJ07_001469"
     CDS             complement(8988..10568)
                     /locus_tag="IWJ07_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAP9333055.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(10561..11304)
                     /locus_tag="IWJ07_001470"
     CDS             complement(10561..11304)
                     /locus_tag="IWJ07_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9333056.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(11480..12256)
                     /locus_tag="IWJ07_001471"
     CDS             complement(11480..12256)
                     /locus_tag="IWJ07_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9333057.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(12504..13340)
                     /locus_tag="IWJ07_001472"
     CDS             complement(12504..13340)
                     /locus_tag="IWJ07_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAP9333058.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(13351..14280)
                     /locus_tag="IWJ07_001473"
     CDS             complement(13351..14280)
                     /locus_tag="IWJ07_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAP9333059.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            14472..14825
                     /locus_tag="IWJ07_001474"
     CDS             14472..14825
                     /locus_tag="IWJ07_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP9333060.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(14899..15555)
                     /locus_tag="IWJ07_001475"
     CDS             complement(14899..15555)
                     /locus_tag="IWJ07_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP9333061.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            15781..17715
                     /locus_tag="IWJ07_001476"
     CDS             15781..17715
                     /locus_tag="IWJ07_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9333062.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(17801..19024)
                     /locus_tag="IWJ07_001477"
     CDS             complement(17801..19024)
                     /locus_tag="IWJ07_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAP9333063.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(19018..20916)
                     /locus_tag="IWJ07_001478"
     CDS             complement(19018..20916)
                     /locus_tag="IWJ07_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAP9333064.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(20910..22028)
                     /locus_tag="IWJ07_001479"
     CDS             complement(20910..22028)
                     /locus_tag="IWJ07_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP9333065.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(22111..22944)
                     /locus_tag="IWJ07_001480"
     CDS             complement(22111..22944)
                     /locus_tag="IWJ07_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9333066.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(22937..23854)
                     /locus_tag="IWJ07_001481"
     CDS             complement(22937..23854)
                     /locus_tag="IWJ07_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9333067.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(23877..24056)
                     /locus_tag="IWJ07_001482"
     CDS             complement(23877..24056)
                     /locus_tag="IWJ07_001482"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333068.1"
                     /translation="MNKINENTSNTVLPATYSDATSYLTTAAYNYWQTGDFENSYLKE
                     AENFAHKYGVKLTGD"
     gene            complement(24081..>25184)
                     /locus_tag="IWJ07_001483"
     CDS             complement(24081..>25184)
                     /locus_tag="IWJ07_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9333069.1"
                     /translation="VIALLNITLLFSLSACGKTNEEVTANIADPMIKKATTLEVYVAN
                     GKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPDIISMADT
                     SAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWASDFTPALC
                     YYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFINMLAQQN
                     QSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMFVAGGWYA
                     TNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQQFLTFML
                     TDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT         7768 a         5026 c         4296 g         8094 t
ORIGIN      
        1 ttctatttat ctatctaaca tatcctttat tcttctactt ttctttcttt agcatataag
       61 agatacgcat agtaatcaag caaggattgt tgatataaaa agaacaaata ccgctatttt
      121 gatttctttt gactctatta ctcatataaa taaaatttat tatacctgct ctttttgact
      181 atatcctcat ctgactccta tacttcgttt gtattaaaac ttaggaggaa tcattcatgc
      241 ttacagggct aaacttatta aataatcctt ttctaaataa aggaacagct tttacaaaag
      301 aagaacgggc aaaatacgga atcactggta tgcttccaag tactgtccaa acattggaac
      361 aacaatctgt ccaagcttac ggtcaatact taagcaaaca gaccgatttg gaaaaacgta
      421 tcttcttaat gaatctattc aatacgaacc ggacattgtt ttataaattg atgggcgaac
      481 atttagttga attcatgcct gtcgtttatg atcctgtcgt tgcggattca atcgaacaat
      541 acaacgaaat tttcttgaaa ccacaagatg ctgtatttct ttcaatcgac gaaccagaaa
      601 atatcaaagc aagtttgaaa aacgcagcag atggacgcga cattcgtttg atcgttgtga
      661 cagatgcaga aggaatctta ggtatgggtg actggggtgt gaacggtgtt gacatcgcta
      721 tcggtaaatt gatggtctac actgccgcgg ctggtatcaa tcctgcacaa gtcttgcctg
      781 tatcaatcga cgctggtact aataacgaag aattattgaa taatccatta tacttaggga
      841 atcgtcatgc tcgtgtagaa ggcgaaactt actacgaatt cattgatcaa ttcgttcaat
      901 cagctactga attattccca gaattgttat tacattggga agattttgga cgaggaaatg
      961 ctgcaacgat cttagaaaaa tacgaagata aaatcactac tttcaatgat gatatccaag
     1021 gcacaggaat cgttgtacta gctggtgtat taggtggatt gaatatatca ggtgagcaat
     1081 tgaaagatca aacgatcttg acatttggtg ctggtactgc tggtgtaggt atcgcaaaca
     1141 tccttttaga tgaaatgatc cgtcaaggtg tacctgaaga agaagcacgc caacatttct
     1201 atcaagtaga taaacagggt ttattgtttg aagatacaaa aggattgact cctggtcaaa
     1261 tcccattcgc tcgtaaacgc tcagaattca ctaatgctga tgaattgaca aacttagaag
     1321 cagttgtaaa agcaatccat ccaacgatca tgatcggtac ttctacccaa cctggagcat
     1381 tcactgaaga aatcgtgaaa gaaatggcgg ctcatacccc acgtccaatc atcatgcctt
     1441 tgtctaatcc aacaaaattg gcagaagcaa aagcaaaaga tttgattgaa tggacggacg
     1501 gaaaagcttt agttggaaca ggtatccctg ctgctgatgt ggaatataac ggtgtaactt
     1561 accaaatcgg tcaagcaaat aatgcgttga tgtacccagg tttagggctt ggtttgattg
     1621 cttcaacagc tacacgagta aatgctgaaa tcatttctca agctagtcga gcattaggcg
     1681 gaatcgttga tgtaacaaaa ccaggagcag ctatcttgcc tccagtagca aaaatcactg
     1741 aattctctca aacgattgct gaaactgtcg caaaatcagt tgttgcacaa aaattgaatc
     1801 gtgaagaaat cacggatatc aaagaagctg ttgaatctgc taaatgggtt cctgaatata
     1861 aatctttaga agattaagat tgttatagta aaagaacata cagaaacttt gtatgacgtc
     1921 taaaaagaaa aaagcgactg cggcaaaaca gcagccagcc aactactgct cttttgccgc
     1981 ttctctttgg cttttttcat acgatacaga ataagaaagg atctgtaaaa atggcacaaa
     2041 aaacaacaac cgcaactgca gttgaggaaa agaaaaaaga agggttttgg tcaacaaaaa
     2101 ttaccggcgt aagtttgccc ctttatttga tcatggtcct tgtcctgatc attgcaatgg
     2161 ctttaggaaa attaccgacg aatatgattg gcccaatcgc tgttttggtc attttcggta
     2221 atttatttca ttttatcgga acaaaaatcc ctatagtcaa aagttattta ggtggtggct
     2281 ccgtttttgc gatctttgcc tctgccgcga ttgctacatt tgggatctta cctgagtatg
     2341 tagtaaaatc cacagaaaac tttgtcagca atatgggctt tttagacttt tatattgctg
     2401 ccttgattac tggtagcatc cttggtatga accgtaatct attgatgaaa gcttctgttc
     2461 gttttattcc cgttgcactt atttcaatgg tcgtctcctt ttttgccgta ggactcgtag
     2521 gtatgcttat cggaaatggt tttgcaaatt ccgttttata tatctcattg cccgcaatgg
     2581 ctggtggtgt tggagccgga gctgtacctt tatcaacgat ttatgcaaat gttttaggta
     2641 cggacgcttc ttctatcatt tcccgcctga ttcctgcgac agcaatgaca aatgtcctag
     2701 ctatcattgg tgcagcgtta gttgctcgtg cgggacaatc aatgccaaaa ttcaacggta
     2761 atggtaaatt aatggaaaaa ggcgaccttg gcgatggaaa acctagaaat gtaaaacctt
     2821 ccattccaca attaggtgtc ggtttgatgg tatcattgac tttcttcatc ttaggacaaa
     2881 tctgtaacta ttttgttcct aaagtccatg cttacgcatt catgatcatt atcgttgtta
     2941 tctgtaaaat cgcaaaccta ttaccagaat attacgaaga tgcagctatc atgttcaaca
     3001 acttgattgt caaaaacttg acagcagctg ttcttgccgg tatcggtatt gcgttattga
     3061 atctaaatgt attggcttct gctttgacat ggcaatttgt tgtcttatgt ttgacaagtg
     3121 ttatcgcgat ttcagtagtt tcaggatttg tcggtcgttt gtttggttta tatccaatcg
     3181 aatcaactat tactgctggc ttatgcaaca actcgatggg tggtacagga aatgtagctg
     3241 tcttatctgc agctaatcgg atggaactta ttgcttttgc tcaaatgggc aatcggcttg
     3301 gtggtgccat cgttttgatc atctcaggtt tcttgatgca attactttct tagtcaaaaa
     3361 aaatacctga tcatcaagta tcggcttgat gaccaggtat tttttgataa aaatttacag
     3421 gattttcaaa aatctttttt gcattttatc ggaatgataa ccgatgcgtt cataaaatcg
     3481 gtgtgcttct actctgtatt ctgcagaatt aagtcttatt gctgatattc ctcgtctttt
     3541 cgcttcttct acgccagtca tcaaggcctt tccgattcct cttttttcag catgtttaga
     3601 cacagcaagt gccataatat tgaacatcgg atcgctgaaa atcgattcat aggactccgc
     3661 ttgtacatag ccagctgctt ttttagaagt aggatcttca tagaccaaga aaaaattttt
     3721 ttctggctcc ttcaacagtt tcttcatttg tctcaccgta acggttaatg gtacatcata
     3781 tcctaattgt tccgcattga tttctcgtag tgcttctgca tctcctaaac gcatttcccg
     3841 caacatccaa atcaccagcc aatttttatt tttttaaatg aattttttta tcacagtata
     3901 ctcctttttt cacttaaaaa aagacttttc agtgattttg acttgcttga atccagatcc
     3961 ttctaatgtt tgcaagatga cgaaagcatt gtaaagattg atcaaaagaa ccacgatgac
     4021 aatcgcttga aaaataaagg tcactttttt tggagacaaa accctgctaa atcttgctcc
     4081 ccacaaacct ccaataacag cagcggggat aacataaaac aacatagata aatcaaagcg
     4141 cataaagcta cttgttaaag caatcgttac caattttgcc agttgagaaa agaaaatcgt
     4201 agatagagaa tatagtgttg cttcttttat tggcaatgca aacatcagca tcaacaaaga
     4261 aacattgact ggtccaccac caatacctaa aaaactagct aaaaagccca gaaccccccc
     4321 acatataagg taccaagtcc atgacgtcaa atgaaatttt ggctgatgat gcttggtgta
     4381 gaagaacgca aaaaccaagg tcaataccgt caaaaaaatt tggatcagct gcacatgctt
     4441 ctcgttttca aataactgta aaaaaacttc gaatacaatg ttgccgacta ctcctccaac
     4501 aacagctcca ctagataccc aaccgacaat ctgccaattc agtgatttcc cgcttgatac
     4561 ttgtcgcaca gtcgaaacaa tggacatcgt gaataccgca actgttgagt aaaaagaaat
     4621 ccctgcaaca gaatgggcgc cgataagatc aagaatcggt ttgatcaaca cgcctccacc
     4681 catcccggaa atagcgccaa ctgtgttcgc cagaacgatc accacaaaat aaatcagacc
     4741 aatcatcttg tatttccttt ctcttgttta tatcttttag cgattgttac tcttttcttt
     4801 tattatacag taaaagaaac aaacgaggct tcgaaagctt ttctactttt taaggaaatt
     4861 tagtgctaaa cgaaaaaacc gggctctact tttcagagag tccagctttt cttacacctt
     4921 atcgattctt ttgttctatt cttcttccgc catgaatgta ttgaatactt cttcgatcat
     4981 atcccattct gcgtctgttt cgatttgttc taactcgcct tctgttccat cttcgttttc
     5041 gatgtatgca taagcttgta attctacatc ttcatcttct gaaacaccag ctggatataa
     5101 aagaacatag ttacgtccaa attcttcttg tccgtcaact gttagcaaga tttcgtataa
     5161 tgtttcattt ccttggtcat ccaccaatgt gatgtgttcg tgtccttcgt gttcgtggga
     5221 atggttatgt tctgtcataa gattacctct tttcttttta ttattaaaat aattgcttac
     5281 ctgagccgtc taaataattt tgtaaaatca tcacagctgc taatttatcg atgacttttt
     5341 ttcgtttcgc tcttgaagta tttgcctgct ctactagcat acgttctgcc tgaacagtgg
     5401 ttagtcgttc atcttgataa tcaaccggca aggagaatag ttctttgatc ttctctccat
     5461 atgacatgga agcttctgcc cttggtccga tggaattgtt catattctta ggtaatccaa
     5521 ctacaaaacg gtctacttcg tactgcgcca ctaattcctt caaccgatca aaaccaaact
     5581 cttcttctgc ctcattgatt cgaatgattt ctactccttg agctgtccag ccaaagggat
     5641 cgctcacagc aacgcctact gtcttagaac cgacatccaa gcccattact ctcataaatt
     5701 cactccatga tttgagaggt agtctttgac aattacttcc atgatttcat cgcgttcgtg
     5761 acggcgaatc aaatttcgtg catcacgata acgaggaatg taggccgggt ctccagaaag
     5821 caagtagccg acaatctgat tgattggatt gtagcctttt tcttccaaag cccgataaac
     5881 aatcgctaat gtttcgctca cttctttttt gcgactatcg tcaaaaccaa aacgtacggt
     5941 ttcatctgta aaacccatga ttctacacct ctttcccgca aggtaattcc agcttacctt
     6001 ctacaccctt attttactcg aagaaaggga aaactacaaa caaatattcc aaacttctga
     6061 ataaattaag aaacacgatt tgtctgattt ttcctttttt ctgtggcttt ttcaaaagcc
     6121 ctctttccat aaagtatacg ctatatttct ttgaaagtat gtttttacac aattttttca
     6181 taaagttcat aaaaaattcg aattgctgct actaattttt cgttttcgtt gcttgccctg
     6241 tctcataatt gatggtgtag ccatcttgaa cattgtagat aaagacattg aattcaattt
     6301 ttttgtcttc aatgctttct gcttccaact gcacacctct tgctaccagt tcatttccct
     6361 cgaaatgagg agtgacacga tagcgtacat gatgatttgt ttccttgata tatgccgcaa
     6421 tatcattttc gtgggcaagc atgtacggag cattcaacga acgtgtccct gtcattaagt
     6481 ttttaatgtt attattttga ccagtcaact ggaaaccaat caaatggctg cggttgtata
     6541 accattgtcc gtcacttaat ttcttttgtt tccaacctgt cggatcaata tacaaaggtt
     6601 ctctcttttc agtaggaaac aagtcttttc ccaacatcgc attggcttgt cctactctgt
     6661 tcaatttgtc tatatttgaa tagctttccc atgtcccttt ggaaagacta agatcctctt
     6721 ttgtaaactc cggctgattc ccattgattt ctatttcttg atgttttcca tcatattctg
     6781 gcacatccgc tacttgagaa gcttgcggtg tggaatcaaa aatggttgta atttgttgga
     6841 agatatctga atctttttgt gtggtggaaa taccgagtgc ccctgcaatc acagcagcga
     6901 cgatccccac gatcgccttg gttgtttttt tgctcatttc tttactcctt tactaacagt
     6961 tccctaaatc ttaacatatg ttcccctata aataaatact ggaaagaaat agaaacagta
     7021 aaaaaataaa accagttttt agacggctta agaatacaaa aaaagagacg gattcatcgt
     7081 ctctactcaa tcacaaaaca ctatttacaa ggtatttatt atcattcaaa aaatttttct
     7141 taatactcaa aactaaacgg cttctgtacg acctcttggt aaacagtgtt caaaagcttg
     7201 catctgcggt aataagtcca accaagtcaa aaacataaga agcttttttg cttgactatt
     7261 cttattactt gatgcagggc agctttttca cagcctcttt taagcgggtg acgggaatcg
     7321 aacccgcgac atcagcttgg gaagctggga ttttaccact aaactacacc cgcgatacaa
     7381 aaacagtata cgcctctttc actccttcct caagtttttt tattctttac gcttccgata
     7441 tgttttgtag taaaataaaa ctaatcaatc aattattgga gaagatgcta tgacgggaaa
     7501 agaattagat ctgttgctgg acacttgttt actcgctggg aaaatcatga tggaaagcaa
     7561 tgcggagatg tatcgggtag aagatacgat gagtcgaatt gctttagctt ctggaaattt
     7621 ccgtttggtc agctatgtca ctcaaaccgg tctctttatt ggtcttgatc gtacctcgac
     7681 catccgaatg gaacaaatta ccaaccgttc gattaattta gaaaaagtag taaaagtaaa
     7741 tgatctttca cgtaaatatg ttgcttcaga gctaacatta gaagaacttt actgcgaact
     7801 gcaagcaatc gaccgagacc gttctttctt tcctgtctgg ttgcaaatcg ttagcgcggc
     7861 agtgatcagt ggaacaatta tggtgttatt tggcggaaag atgaccgatt tacctgtgac
     7921 tttagtcatt ggaggattgg gatacgcagc ttatctatat agtttgaaat tttttagaat
     7981 caaattttta tcggaatttt tatcttcttt attgattggc atagccgcta tctacagcgt
     8041 acgttttggc ttaggaagca atcaggatct aatcattatc ggatgtgtga tgcctttagt
     8101 tcctggagtt caaattacga acgctatccg tgatttgcta gctggacatt atgtctcagg
     8161 agtttctcgc ggaacagaag cgatgatgac tgctacgatg atcggttttg cgattgcttt
     8221 tattttccag cttttttatt aggaaaggaa cacaagtatg gggacgatat ttattcagtt
     8281 ttcttttagt tttttagcga ctgcggcatt tgccgttatt acaaatgtac ctaggcgttc
     8341 attgatttgt tgtggtcttt ctggtgcttt tggctggatg atctactgga tctgtgttca
     8401 aataggcgga tctgctgctt ttggttcatt gcttggttct ttaggagtag cattcatcag
     8461 cgatcttttc tctaaacgtt tgaaaatgcc cgtaacgatt ttcaatattc ccgggatggt
     8521 tcctctagtc cctggtggtt tagcttatca agcggttcga aatctcgtta ctgaatccta
     8581 tcgagaagcg gctcattatg cggtccaagc aattatgatt gctggagcaa tcgctttagg
     8641 tcttgtatta tcagaagtct ttaaccataa catccgtaat ttcaaagtaa agcgtgacgc
     8701 aatcatgatg aaacgaaaaa aacggctttt taaataaacg aagtgtaaca aagaaaaggt
     8761 tgtgaaagag acgttcagcc ttgagcgatg acaaaatatc tcacaaacat tgcgacaagt
     8821 aaccgcagga gcaataagaa gtaattatga gtattaaaaa aagttttgta aaaatagctt
     8881 ctcacatttt tacataaaat tgacttaata ctgagaaact gacttttgaa caccctctat
     8941 ctgtaaaaaa ggggctgcga catgattttt gtcacggccc ctcttcttta ttcaaatttt
     9001 ttccaaaacg ttcgttgata ataaacgatc catagagcgg aaagtattag aactaaaccg
     9061 atcacaggaa tattcactaa ccaaaatgga taaaacaaag ccaacattcc gtataagacg
     9121 ataagaataa tactgatgat caatgaaccc atcatagttg cgactaaacc caaacttcct
     9181 gaacctctgt taaataattg gttaatattg gtccagttca gcagcagcaa ccgataatct
     9241 ctagcgaaat aacgtagtga caataagtag cagccgaata aagaaccaaa tagcaagcta
     9301 gggataaaaa acaaaggcag ctgaaaagac agtccgctta ataatgcgat cccaccagtt
     9361 aaaaaactct gcaatatcca tccgaatctg aacttttctt ttaaatattg gttcatagag
     9421 attggcaaag aacggataaa cagaaaattt tcttgatcca aagaaataag attgctgata
     9481 aaagaagtct gattaactgt taagaaagcc aatgcgatcc ctaccaggaa aacaacaccg
     9541 attaacttat tatctaaatc agttaatttt atttcgcctc ctagggcaaa agcaagaata
     9601 aaaatgattg gagttaacaa ggaagaagaa agaacttgca taatcaaatt aggttctttt
     9661 actaattgag cattgtaact aaataaaagc tgatgcacat tttggttctt tttatgggtt
     9721 ctttttattt tgaactcact gtttgaatca gtcagttgct ctgtaagctt tggtactatc
     9781 agctgtttta cagcaaggaa aaatcccaga gccgtgatag ctagtattcc ccaacccaat
     9841 aaaccttgtt gagaaagtgg ctgactggca gcatcataga atggcaataa tatcgaaatc
     9901 ggatgccgat ctgttaactc attttcaata gtccccgttt gatgattcat ccacatgatt
     9961 ccagcaaatg cgatcagcat cgagctgcca agcaagatac tagaaactgt ttttctatgt
    10021 tttttaaaca ctgctgttcg agccaaacca aaaacaatca aactagccag acaaaaaaga
    10081 ataatcaaaa atagagaaaa caaaaggatt gagataatga tagtaagcaa aatccctaga
    10141 tcagctcgcc atccggttaa aataaacatg actagaagcg gaaaaacaaa aggaatgatg
    10201 gtcaaagcaa ctaccaaaat ctttgctgaa aaaacctgtt cttgtccaaa aggcagaggc
    10261 aaaaatactt gcaggtcatt tccttcaaaa aagatattat aaatgactga tatactttga
    10321 gttaaaccta acataccaaa aagtgccacg taataagtaa agaaacctgg caatttaaca
    10381 aagttcatag caaacatagc aaatccgtaa atcagtaaaa aaatcagccc tgaaaaaaga
    10441 ttttgagaca aaagatagat agttaatcgc tttccctttt ttcctttctc tcttgcttta
    10501 ttcgtgagct gtggattaac atataaaaga ttaactcgaa ttaattctaa taattgtcgc
    10561 ttatacatac ggtccatctc ctgtcgcttg ccttccagcc attttcaaat aaatcttttc
    10621 tagcgtttca tcaggtgagc cattcaataa ttcactaact gccccctgat aaatcaattc
    10681 acctttcttc aaaatagcca gtttatcgca taactgttgt gcggtatcca atacatgtgt
    10741 agaaaaaatg actgtttttc cttttgcggc atgagccttc atcatttctt tcaaatcaaa
    10801 tgctgcttga ggatcaagtc cttgcaaagg ttcatccaat acccaaatat ctggatctgg
    10861 cagcaacgtg ccgatcaaaa tcgttttttg acgcattcca tgtgaaaaac ttgcgattgt
    10921 ttcattttga tgactataca tatcaaaaag cgctgtcaat tctgctaaac gcttttcttt
    10981 tttttgtgtg tctaattcat aggcagctga aatcaagtcc caatattctc cagccgtcaa
    11041 ttgtaaaaaa atatctgggg aatcaggcac ataaccgatt ttttgtttga tactcaatcg
    11101 attttccgtc aatttcattc catctactaa aatcgttccc gatgatggtt caataatact
    11161 aactaaactt ttaatcgtag ttgattttcc ggcaccatta tgtcctaaaa atccaaaaat
    11221 ttccccttgt cttatcgaga gagacaagtc ttttaatgct tgttttttcc cgtaactttt
    11281 acttacattg atgaattcaa tcatttctgt actccttttg tttgttatta ctttttattg
    11341 tacccttcgt tattggtgga gtaaatgtca aaaagagtgc ataacaaaaa aggatagaaa
    11401 cgactaggct gctacttggg ttgttctctt ctacaggaaa acactcttct aacaggtaag
    11461 atatgagtct ttgttttctt taaaatgatt cattttctat cttttcttgc ataaaattct
    11521 ttagtttatc aatacttctc tttcccatct ctagttgttc tccatttgca aatgttacta
    11581 tttcgtttac tctgctaata gatgtaatgt tttcagtatt aatgatataa gcttttaaat
    11641 ctttacataa atactcatat tcgagtgatt ttttatattt tttaattgtg ccgtttacga
    11701 gaatctcttc atctttcaat actaggccta ttttattttt ataacctggc atgctcatta
    11761 gataaaggat ttcatcgtaa tggatatagt gatcgctatt accataacgg acagttgtcc
    11821 accttttttt cgtagcatgt tgaacaggat agttttcttt ttctttcaat aagtaacttt
    11881 ggatgctttt tttatttttt tcataagtga attcttttat caaataacca ctaggcgtaa
    11941 tctttcgatt aataacagtt attgctttat tcggatgaga cgttagataa acaattttac
    12001 acttttcatt cttttctcta agcatttcgc ctagttcgat accgctataa taggcgttca
    12061 aatcgatgtc taggaaaaaa atatctgagg aatagatttc attattttta atgaattcaa
    12121 caaattgtat aacgttcaat ccctgaacga tctcaaattt ccaaggactt aataaaattc
    12181 tggattcttc caaaatttct actagcattt tttcaaaaac caaactatca tccaaaataa
    12241 atatacgcgt taccataaaa agtacctcca ctcgatattt tactatcaaa atcatttcat
    12301 acaacttttc aacatacatg ttaggaataa attgtaattc tcttcacaat cttttccaac
    12361 acctttgaaa ccgatatcaa atactttcag gatgctgctt ctaaaataaa tatcaaacaa
    12421 atgaatacat catgcaaaaa aagccagaca accgacgtcc ttgcgtgtca gctgtctgag
    12481 ctatgtttat ttattttgtt cttttaaaaa gaacgataca catactgatt tttcggagca
    12541 gagactttct cagcgtttgt aacaactaca gatgggattt cacgcttgaa tggttgataa
    12601 tagtctgatt ggatggttcc ttctatttta taccattcat catttttaac cgttactcct
    12661 tccggcatat gcgtcaacaa accaaagact cctgaatctg ccacacaatg gataatgccg
    12721 aatcggaaca agaagatatc tgtattgcta tcgttcggtg tttggtaaac aaatccttca
    12781 tagctgattt ttttgccaat gaattcgcta gggtaattat aaattagctc cataacttct
    12841 aaatagtttt catctgtgat cttgatagtt tgcttatccc catacttttc tttcaactca
    12901 ttcatttggt ctaagtagtc tgatttgttg aaataaatac tggtatccgg ctttaagtat
    12961 tgtgtctgca tctccggatc ccctactgat tctttactta ctgggaaatt aaatcctttg
    13021 gcttctacga tcgtagtgtc gagactaact gttggaaaca acgtccccac gattactggc
    13081 aaagctagca gtacataagc gattccacgt tgataaggtt ttgacaaccc atggtcatga
    13141 tcgtgctcgt gtgccttttc ttctgctttt ccgtttttga cccacaaata cagctgaact
    13201 aaagccaaaa gagcagataa aatcattgaa agaatagcta agtagcgata atggacattg
    13261 atatattgat cgagtttgcc agaaacttgc aagtacatca tcaattcgaa atacccagat
    13321 aaaatcaaaa accgcagcat attttccctc ctaaataaat aatgtgaatc caataacgct
    13381 aagggcaatc agtccgatca gcgataaaat aaagcgtcct gtaaaatatc gtttcatcat
    13441 cagtagattt ttgatatcga ccattgggcc aaagaccaaa aaggcagcta caggtgctgt
    13501 gccgaacaga cttaacaaag aagatcctat aaacgcatct gcttctgagc aaagagacat
    13561 cgtaaaggcc aataacagca tgatgataat ggctaaaatc tttgttgagc cgagcgttag
    13621 cataattcta gttggtaaat aagtctgcat cgcagctgcg attaatccgc caatgatcaa
    13681 ataacgacct gtatcaaaaa attcgtcgat tgaatgtgtc aacacatgcc cagtttgatg
    13741 gatcagctga ttttttggtt cattcttctc actttgttct tcttcatgga catgatgatg
    13801 gtgattttct gctactttag acgtgatttt ttctttcaat acagcgtctt tttgaaaata
    13861 ggcaagccag acaccaatca caagggcaac aaaaaagctg cctatcaccc gccagcttgc
    13921 catctgccaa gaattgccaa atgcgataaa agtggaaaaa atcacgattg gattgatgat
    13981 cggagcagtc agcataaaag caaacgctgt gtgaactggt acgccttttt taacaaattg
    14041 atgaacgatc ggtacgatgc cgcattcaca tgaaggaaag aaaaatccta agatcgaacc
    14101 agtaaaaata gagaagatac gattttcagg aagccatctc ttcacacgtt ctggggtcaa
    14161 aaatacttgt aaggcgcctg agataagaca gcctaataat acgaaaggta atgcttcaat
    14221 aacgatcgat aagaaaatcg tactcatctg caaaaccgaa tttggaataa aagaaaacat
    14281 gagtttcctc ctttttattt ctagttcatt cgctttgcaa atatacgctt ttattcaaaa
    14341 aaaacaagtt gctctgtaaa tttttacaca actcttattt tttaccaaag cacttcaatt
    14401 gactttttct gttattccca ttaaattaag gtctcataca tatcacttct atcataaaag
    14461 ggagaataaa aatgaaacaa accactaact tgactgaagt tcaagacata ctgcaacagt
    14521 catccgttgc cgtcatttat ctttcgatgc ctaattgcag tgtttgtcac gctgttcgtc
    14581 cacgactaga ggaacttctc acacattatg atattccggc acttcatttg gatgcatttg
    14641 aaacaccaga agtagctagt aaatttgaag tgctgactgc gccagttgtc ctgatttttc
    14701 atcaaggaaa agaagtcttt cggcaagcac ggttcatcga ctttaaaaaa atccgttatc
    14761 tactagatga actgacagca gaagatgatt ccctttctta tgaagaaatc ttccgaacaa
    14821 aataatcgtt gtccaaacag aaaaaagcga ttgaacaagt gaaagttttg ccttcatttg
    14881 ttcaatcgct ttttattttt attctgctgt attttcggaa tgcgcatttg cgtcagaatg
    14941 caagaaatag atcaaggttt gcaattcatt cgtcaaatca acattttgga tcaatacatc
    15001 tgacggagca ttcaatcgag cagcggtaaa gttcaagatt cctttgattc ctgcttccgt
    15061 taactgattg actacttctt gtgctctttc agcgggtatc gttaaaattg ctacttcgat
    15121 ttgttggatc ttgatttgtt ctgacatatc ttccattgga taaacgggga taccgtctac
    15181 gatgcgccca acgatttcct cattcacatc aaaagcacaa ctgatacgta tgctattact
    15241 ttgatggaat ttgtatttta gtaaagcact tcccaagttc cctacaccga tcagcgcaac
    15301 atttgtcaat tgatcgtcgt ttaatgtctt tgcaaaaaag tgcataagat cttctacatc
    15361 ataaccatac ccacgttttc ctaattcgcc aaaataagaa aaatctcgac ggatcgttgc
    15421 actatctact tgaacggctt cacttaattc agttgaagac acttttttct ttccagcatt
    15481 atttaaaatc cttaaatagc gataatacaa aggcagtctt ttagctgtcg cctttggaat
    15541 aatatgatct ttcacgaaat gacctccacg tattgtctaa ttacatcttt gatttctcca
    15601 tcatagcagt atttatagga aaaaatcaat tccattgctc agatattgtg aaattttatt
    15661 ctttattcat ttatttatca tttctgcaaa tctcgcagta taatggaatt tatggtagac
    15721 tactaattga aaaatagttt tttatttgag gagaattcac aaagtagaaa agaggattcc
    15781 atgatattat tacaagccaa tcagattgct cgccattttg gtgcagaagt cttatttgaa
    15841 aacatacatt tagaaatcag tacaggcgct cgtgtcgctt tagttggcag aaatggcgct
    15901 ggaaaatcga ctttattaaa gatcattgct ggaattgaag cacctgatga aggaaagatc
    15961 gctaaaaata aaacagcgac tctcggatac ctcgctcagg acacaggact tgattccaat
    16021 gaaacagttt ggaacgagat gctgaaagct tttgaacaag ttagacaaat ggaagagcgg
    16081 atgcgcgagc tagagatttt cattagcgaa ggtacgccag acacacctgc ttatagttct
    16141 tatttaaaag aatacgaccg gctgcaacac gatttttcag atcgaaacgg ctatggttat
    16201 gaaaatgaaa ttcgttccgt attgcatggg tttaaattcg attcttcatt ttatgatcag
    16261 aaaatcgata ctttatcagg cggacagcgc acacgtttag ctttagctcg catgcttttg
    16321 caacaccctg acattttgat tctagacgaa ccaacgaacc atttagatat cgagacactt
    16381 tcctggcttg agaattatct gcaaagctat actggtgcat tattgattgt ttcccatgac
    16441 cgttattttt tagataaagt agtcaatgaa gtctatgaat taagcagaag aaaaatgcgc
    16501 cattacaaag gtaactattc gaaatatcta gatttgaaag cagaacagtt agcaagtgaa
    16561 tggaaggctt atgaaaagca gcaaacagaa atccataaat tagaagactt cgttgctcgg
    16621 aaccttgttc gcgcctctac cacaaaacga gcgcaaagcc gcagaaagac attagaaaaa
    16681 atggaccggc tggatcgtcc tcaaggggat gaaaaacaag caaactttct attcagtata
    16741 gaaaagcctt ctggaaatgt agtcttgcaa atcgaagatg ccgctatcgg atatgataat
    16801 gtcaccttat ctgaaccaat caatctagat atccgtcgtc aagaagcgat tgctctagta
    16861 ggtccgaatg gtatcgggaa atcgacgttg ctgaaatcaa tcattcacca gatccctttt
    16921 atccgtggaa aagaaacgct agggacaaat gtcaccatcg gctattacga tcaaggtcaa
    16981 gctgatctgc atacaaacaa gacgatccta gcagaattgt gggatgagca tcctaccact
    17041 ccagaaaaag agatccgaag tgctttaggt gctttcttat tttctggcga agatgtagaa
    17101 aaaccaatcc ctctgctaag tggcggtgaa aaagcacggg ttgctttagc caagttagcg
    17161 atgaacaaag aaaatttttt gattttggat gaaccaacaa accatttaga tattgacaac
    17221 aaagaagtat tagaaaatgc attgatcgat tatgacggca cactgctttt tgtttcccat
    17281 gaccgatatt tcatcaatcg gatcgcgaca aaagtcgtcg aactctctga aaatggcagc
    17341 aaagtctatc taggagacta tgattattat ttagagaaaa agcaggaaga agaagaaatc
    17401 gccgaattgt tggcacaaga tgagccagaa tctgctcctg ctcccacgaa caaatctgat
    17461 tattaccaaa gcaaagaaca gcaaaagctt atccgtagcc ttcaacgaaa aattactcag
    17521 gttgaagaag aaatggcgcg gatcgatgaa tggatcgatc agttggaaca agaaatggtc
    17581 gcgccagaaa acctggaaga tcacgtaaaa ctcaatgagc tgaaccaaga tttagaagct
    17641 gctagacaag atcaagaaac aaaattgacg gaatgggaag aactaagctt ggaattagaa
    17701 gaatatgaaa attaacgaaa aaaccaaaac agaaaccatt catgctgttt tggtttttct
    17761 ttttttattt tatcaaacaa aaattccata tttacctttt tcaccaataa gaagaccaat
    17821 tactagtcat tctcactaaa gcttctaaat aatagtaatc accccaaata caaggtgtat
    17881 ttaccccatt atttttgggc ttattataaa cagactgtgt taaaaagcca tcttcttctt
    17941 ccgttgtagc atatctttct ttcaattgtc gcataatttt ccaaacagca gactggtata
    18001 agttcttttc tgttttgtct aatagacctg attctattaa ttctaataat ccacaaacca
    18061 tgatcaccat actagatgta tctttttctt ctctgctaga atcatcaaag atcaaatccc
    18121 aataacaaat ggtatcttct ggcaaccgat tcataaaata attggctaat attttgctta
    18181 aatcaaaata tttttgttct ttcaaataac gataacttaa tgcaaaaccg taaatccccc
    18241 atgcttggcc tctagcccaa caggaattat cactataacc ttgatgcgta cttccatatc
    18301 ttggttttcc atctttgaca tccatataaa atgtatgata agttgaacca tcttctctta
    18361 ctaaataatc tgctgcattt tgaatatgag cctgagcagc ttttttatat ttttgatttc
    18421 cagtttcttc actcgcccaa tacaataatg gaagattcat gcaacaatcc atgatcattc
    18481 taccttgttg tgaaggatca ctaggatctc cccaagcttg tataactcct gccttctccc
    18541 aaaaacgctg aagtaatgtt tctgcagcat caattgaagc ttccttcatt tttcgatttc
    18601 ccgttatttt ataatctgct acggttgata aagaatacaa aaaaccaaga tcgtgatgat
    18661 tagtgataat ttgacagtca atcctgtgga taaaatctat atctgctttt tcagctgttt
    18721 gttgaaataa aggatcaccc gtcttctcaa ataacaacca attaattcct gtccaaaatc
    18781 cttctgtcca accaatattg tctcttaatg gataacgtcc ttttatagga cactcgcttg
    18841 ggaattgata ctgactgtta ctgatacgtt tgattaacct ctccgtgtgt ctgcgacaac
    18901 gctcgatctc attattccat tctttttcca ttaattcttt ataacattca tttatccatt
    18961 tttctggatg tattatctct tcaaacatca ctttatcaag catttcccca ccagccttta
    19021 gcataatttt agtctttttt tattgttttt attttggata gttagcccgg ttgtcccatc
    19081 aaaatcaatt ttgggacgat tgttccatac ttctaaacta taatcccgat cttgatgagc
    19141 aaccactcct gttccgagcc aatgggttcc ttttccaaat gtaccctcta atccaggaat
    19201 aaaagtgttt tcccatgtca tcaagtttgt attgggcaaa cccataatag ctgtggattt
    19261 tcgtttaata gtatcgtcac ttaaacagat aatacctgaa aagccagctt cactcaccct
    19321 atagttttcc ttgtcttctt ctatgattat tccttctttc tcaggagaac attcaatagc
    19381 aaaccctgtc tcatatagaa catattctgt atctaattct actttgtgta cgcgaatctg
    19441 ccagccttca aaaggaataa gccaagttct gatttttgcc tgtaaagtag gttcccatgt
    19501 agaggcaagt acctctttct tacatacaat gtctcgtaca ttatttcgaa cgatgaactg
    19561 tccataggtt ttgatgctct cattcttaaa actggtcgtg atatatgtgt ccgctcgctg
    19621 aatccctagc attgaatcca ttgccatttc tgctttcaat tgattcgctc gcggtatcga
    19681 aaaaccaaat tgactagaat aagcaaattt taaatatttt tcattggtca atgtatggaa
    19741 atttggccct aattgtcccg cattcaaaaa aaatgtatgt ccattatcat gttgtatgag
    19801 caaattggct tctgaaagaa gtttagttcc ttcacgaata ggcataggca tttctttagc
    19861 cgtccaatac tcatggtctt caggtagcgc caacaataaa aaaatcttat tcagccataa
    19921 tggagataac gtactattat agggttcagt caatgtcaat tgagggtagg tataaccaat
    19981 attcaggata cctttttcat caaatatttc tttttgcatc caccatctta tattccgatt
    20041 gactaacccc ttgatttcac ctaatttaaa aggcaaaaga tcggcccaca acatagctga
    20101 ccaaaagctt gaaactgcaa aacgataaat catgcttcga ccaaaaggca catttgcacc
    20161 atcttccgca aaaaaatgaa tatagtctct agaaaaatca atagctcggt tcttatatat
    20221 ctggctaatg ggcttttctg gttctagtac gctataaatc aaaccataat aatgaaatgc
    20281 aaaaggatta taataatctt ggcgacctct tgaactgtct cgataccagc cattttgaag
    20341 ataacaattt tctatattat caaatgcttc tttcagtgct gattctttaa ctggataacc
    20401 aaatttttgg ataattgttc cgacaattat cttaaaaaat tgccaattac catctgcaca
    20461 ttcatattca aaaatttgcg agaaccaatg aataatctgg cttttttctc ctgtatctaa
    20521 ttttttccat aataattgtt catgatgata taacgctaat aacattgcgg gcatttctac
    20581 catacgctgg tctctttcat gtatttctcc ccaataatcg ggatgggaag gatctgttcc
    20641 agcttttatt cctgatagaa ttaatttcaa ccatcgttca tctaattggt caattgcggg
    20701 ccccgctccc cataaaaccc gagagaatcc ttccattcct gcaattttat ttccatatcc
    20761 taccccttga tgtatataat caatttgtgc gccacttgct cgataaaacg gctggagtgg
    20821 ttgatttatc tttttaaata agtcaaagta atcctgtctg gtccttaata gtggtactgg
    20881 atgtttcatt gtattttctc ctctttttac taccaaatag ctaattccga ctgttctaat
    20941 cgaagtaagc cttcaataaa aaaataatca gaatagatca gtggcacatt ataagtcagt
    21001 tctctatggt atgcttctgc tccgcctcca atgatcccat cttcatccat attccaattt
    21061 gcatagtgct tttctgtctt ttggagtata ttgatagcag cttcatgata catttttgat
    21121 cgtacagagg aaacatatcc tgctaattcc agaagaccgc atgcagcgca tactccagca
    21181 ctggtatcaa tattcgaatc ttttacagca aaatcaatga gaggaatata accagttttt
    21241 tcgattttct gaataaaatt tactgctatc ttttctgcta tagctagata gtacggattt
    21301 tttgtgtaac gataacttaa cgcgaatcca tataaagccc atgcatttcc tctgctccaa
    21361 caagaattct cattgtatcc ttgcccgcca agtaaccgaa tatatttccc tgattctgca
    21421 tcaaattctg cgatatgccc ggttgatcca tctttcctca ctagatactt agctgttgta
    21481 tctgcatgct gccgagcaat tttagagaac ctcggatcac cagtttccct cgaagcccaa
    21541 tacagtaatg gtaaattcat catactatca ataatcatcc aaccaggttt atggtcattc
    21601 catgctacta aatagttccc tgaaatatta tagcgtccag caagtaccgt agctgcatgt
    21661 aaccctagtt gtcgactatg tttgttttga gactgttgat aatgtgcaat agccgatggt
    21721 aagaataaaa aacctacatc atgatgaacg tctgaaaaat ggtacagggc agattctaat
    21781 aattgttctt gttgtattgc tgcctctttg aacttttctc gttttgtctt gtgaaagagc
    21841 tgccataaaa taccactcca aaaaccattg gtccaccagt cgattcctct gtcctttatt
    21901 acgtctgtat attttccgtt gtaagaaata tatggaattt tattccccat tctgtctaat
    21961 tccgcatcta tcttttcaga agctacattc aaaatttttc ttgaccattc cttcgtctgt
    22021 tcattcatgg atatcccgcc attcttttaa tttttatgaa tgtggagttg ctcctagaac
    22081 gaactagatg gttaacattc gttatttttt ttacttattt gcagctccaa tgtcattctc
    22141 cttaaattta gactgcgccc acacataaat caagaccatt gggatcactg aagttgctaa
    22201 aattagcata attgcgccat aattaattgg ctcacctgta ctagaaaccg tcatattttt
    22261 gataattaag ggaaccgtat atttatcttt atctgaaagc atgactaatg gaaatagata
    22321 ttgattccaa cttcccataa agataagtaa accactagta ataatcgtag gtgataaaat
    22381 cggtacagcg attttcgtaa aaatcaccca ctcattggca ccatcgattc tggcagcctc
    22441 catcagtgct gtaggaaatt gactgctata ttgtcgcatc ataaacactg aagttgtact
    22501 ggcaatcgaa gggatgatca cacctgcata agtattcgat aatcctaaac gactcataat
    22561 gtaaaataaa ggaatcaatg tggtaaaaaa aggcaccatc ctagatagca taactgtatt
    22621 gaacaacagt ttattcccca taaaacgata ctttgctaat gcataacccg ccattgtaga
    22681 actggctgta gctgaaactg tactgattac agcaataaca atcgaattcc aaataatcaa
    22741 gggataatta tatctcgcca tgatggcttg ccagtttttt aatagcatat ccccaaactt
    22801 gaaattgatt ccgccactta aaatttcttg attactttgc gttgaagcta caatcatata
    22861 taaatatggc atcaaaaata ttaaggcacc aaacacaata atgacgtacg taaaaattct
    22921 gctttgtatc ttttgtttag tcatttttat cacctccgaa tttaaattga atagccgata
    22981 atataacaga tacaaacacc atcgtccagg caatagcaga ggcttttcca aaatcaacca
    23041 tttcaaaact tgttttatat aaattgactc ctacgaccaa agctgaattt tctggtccat
    23101 tggaagtccc aaagaatata tttgggatat tgaaattacc caagccattg atagtggcta
    23161 aaactgtggc cattaaaatg attggcttta gcatggggat cgttaataaa aagaattgcc
    23221 gaaatttcga tgctccatct atttcagcac tttcgtatat ctctttagaa atcccttgaa
    23281 gaccagctaa aaataaaata gtgttatatc ctacactaag ccaaatcatc gtgatgatca
    23341 ttgatattcg tatccaaaaa ggatttgtta accactgtac accgttaatt cctagcgcat
    23401 tcaaaaatcc attgaataac ccagtcggat tgaaaaaagt catgaatacg gaagctacag
    23461 ccaccgttga tgtaatggtt ggaatataat agacagttct aaaaaatcct tttcctttta
    23521 actgtgctga attcaataaa acagcaaagt aaatagctaa tagcatcgtt attggaattg
    23581 atcctgccat gataatcaaa acattcgtgt aagatttaat aaacaatgga tctcggaaga
    23641 tagctagata attgtcaaat ccaacaaatt caaatgagta gccaattttc ataaaactaa
    23701 aagcaaacga gataatgatg ggaatcaaaa tcgtcacaat aaagaaaata acaaaaggtg
    23761 tgaccaatcc ataagcactt tttttatgag acgtatctgt tgcttttttc ttgctgttct
    23821 ttattgtctt gcttggtaac gaagattgca tcatcgttaa gtcctcctaa tattttttaa
    23881 tcaccagtta attttacgcc gtacttatga gcaaaatttt ctgcttcttt tagatatgaa
    23941 ttttcaaaat cacctgtttg ccaatagtta tatgcagcgg tagtcaaata agacgtagca
    24001 tctgaataag tggctggtaa aacagtattt gatgtatttt cattgatttt attcaatagt
    24061 tcatagtatt tttgtcctcc ttagtagtca aactttttct cagctgcttc tgtttgatag
    24121 gccaatgagt tagatgtagc cacgcccagc tccagtgcgt tggctaagca ttcttcatct
    24181 gtcagcataa atgtaagaaa ttgttgtgcc acttgtggat ttttggcatc tttagggaca
    24241 taaaaacttg atcctcctga tactgcacct tttcctggat tttcttttga aaaaggaatc
    24301 agtggagcca tgcgccattt gtcagatgca tcaggaaaat tcaaagacat attcgtagca
    24361 taccagccgc cagcaacaaa cattgcagat tcttgaaatg ctttctcagc atcttgtgca
    24421 ccgtagaaat gtacaatatc tgagtcgatc atttctttga ttaactttgc agcatgtttc
    24481 gcttctttag tattcagacg gatatttcct tcttcatcaa gcaaagattg attctgctgg
    24541 gctagcatgt tgataaacaa ttcttggtcc ccagattctg gcaaggcaat tccaaaacta
    24601 ccagtttttt cttttaatgc ttttgccacc tggatatatt cttcccaaga actgatttct
    24661 tcaaaatcta cacctatttc agcaaatttt tttggttgat aataacaaag tgctggggta
    24721 aaatcactag cccaaggaat aatcgtttca ttactcgttt gttgacgcaa aatattcaat
    24781 ttttgatcat aaaactgatc gccatatttg tcataaaatc catattcatc agcactataa
    24841 aaagcatctt gaaatttttc cagcacacct gctgctgaag tgtctgccat ggagataata
    24901 tccggcatat cttcgtgtgt taccaatttt ggggtgatca tttgtactgt tgctgttccc
    24961 gaagctacat tcacaatttt taagtcaata tttgctccag ttttgcggtt atagattttt
    25021 gcagcatttt caacatagtt tgtcagtttg ccgtttgcga catatacttc taatgtagtg
    25081 gcttttttta tcattggatc tgcgatgttt gctgttactt cttcgttagt ttttccacat
    25141 gcacttaagc tgaataatag ggtaatgttt agcaaagcta tcac
//
LOCUS       DACDPS010000034        24495 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.034, whole genome shotgun sequence.
ACCESSION   DACDPS010000034 DACDPS010000000
VERSION     DACDPS010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.034"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(55..789)
                     /locus_tag="IWJ07_001484"
     CDS             complement(55..789)
                     /locus_tag="IWJ07_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP9333070.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1116..1526
                     /locus_tag="IWJ07_001485"
     CDS             1116..1526
                     /locus_tag="IWJ07_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAP9333071.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1519..2220
                     /locus_tag="IWJ07_001486"
     CDS             1519..2220
                     /locus_tag="IWJ07_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAP9333072.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2220..3806
                     /locus_tag="IWJ07_001487"
     CDS             2220..3806
                     /locus_tag="IWJ07_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9333073.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3825..4046
                     /locus_tag="IWJ07_001488"
     CDS             3825..4046
                     /locus_tag="IWJ07_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAP9333074.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4043..4804
                     /locus_tag="IWJ07_001489"
     CDS             4043..4804
                     /locus_tag="IWJ07_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAP9333075.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5184..5693
                     /locus_tag="IWJ07_001490"
     CDS             5184..5693
                     /locus_tag="IWJ07_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP9333076.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5768..6307)
                     /locus_tag="IWJ07_001491"
     CDS             complement(5768..6307)
                     /locus_tag="IWJ07_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAP9333077.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="IWJ07_001492"
     CDS             complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="IWJ07_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAP9333078.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7374..8171
                     /locus_tag="IWJ07_001493"
     CDS             7374..8171
                     /locus_tag="IWJ07_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAP9333079.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8199..8834
                     /locus_tag="IWJ07_001494"
     CDS             8199..8834
                     /locus_tag="IWJ07_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAP9333080.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8854..9627
                     /locus_tag="IWJ07_001495"
     CDS             8854..9627
                     /locus_tag="IWJ07_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9333081.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10035..10832
                     /locus_tag="IWJ07_001496"
     CDS             10035..10832
                     /locus_tag="IWJ07_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAP9333082.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10810..11223
                     /locus_tag="IWJ07_001497"
     CDS             10810..11223
                     /locus_tag="IWJ07_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333083.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11207..13852
                     /gene="mutS"
                     /locus_tag="IWJ07_001498"
     CDS             11207..13852
                     /gene="mutS"
                     /locus_tag="IWJ07_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP9333084.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13870..15978
                     /gene="mutL"
                     /locus_tag="IWJ07_001499"
     CDS             13870..15978
                     /gene="mutL"
                     /locus_tag="IWJ07_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAP9333085.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16000..16560
                     /locus_tag="IWJ07_001500"
     CDS             16000..16560
                     /locus_tag="IWJ07_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAP9333086.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16645..18633)
                     /gene="treP"
                     /locus_tag="IWJ07_001501"
     CDS             complement(16645..18633)
                     /gene="treP"
                     /locus_tag="IWJ07_001501"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAP9333087.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18856..19569
                     /gene="treR"
                     /locus_tag="IWJ07_001502"
     CDS             18856..19569
                     /gene="treR"
                     /locus_tag="IWJ07_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAP9333088.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19646..20092
                     /gene="msrB"
                     /locus_tag="IWJ07_001503"
     CDS             19646..20092
                     /gene="msrB"
                     /locus_tag="IWJ07_001503"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAP9333089.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20262..20864
                     /gene="ruvA"
                     /locus_tag="IWJ07_001504"
     CDS             20262..20864
                     /gene="ruvA"
                     /locus_tag="IWJ07_001504"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAP9333090.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20877..21878
                     /gene="ruvB"
                     /locus_tag="IWJ07_001505"
     CDS             20877..21878
                     /gene="ruvB"
                     /locus_tag="IWJ07_001505"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAP9333091.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21907..22485
                     /locus_tag="IWJ07_001506"
     CDS             21907..22485
                     /locus_tag="IWJ07_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP9333092.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22537..23019
                     /locus_tag="IWJ07_001507"
     CDS             22537..23019
                     /locus_tag="IWJ07_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAP9333093.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23136..23510
                     /locus_tag="IWJ07_001508"
     CDS             23136..23510
                     /locus_tag="IWJ07_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333094.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            23940..>24495
                     /locus_tag="IWJ07_001509"
     CDS             23940..>24495
                     /locus_tag="IWJ07_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9333095.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDATYIPLKRQTVSKEAIYIAIGI"
BASE COUNT         8032 a         4405 c         5223 g         6835 t
ORIGIN      
        1 ataaaaaaac ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc
       61 cataaataat aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac
      121 tggtacccat gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg
      181 tacaagacgt ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag
      241 tgaagcaact aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc
      301 ttggaagaat ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa
      361 gaattcattg acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc
      421 taacacatgg tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat
      481 caattgtcca atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca
      541 agggaataga ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct
      601 gattccacca aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag
      661 catccagtca ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt
      721 acctagaacg attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga
      781 gtaatccata aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg
      841 aaaagcatcg gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat
      901 aaaaaaatga attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt
      961 taaaagttct ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt
     1021 tttctcaaaa attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta
     1081 gtaaaatgaa agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca
     1141 ttattatttt cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc
     1201 ctggaagtat cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc
     1261 ctgaaaaaat cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc
     1321 ctgctggtgt aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac
     1381 tgggagcagt cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag
     1441 gtgtagaagc tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg
     1501 aaatggagga gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg
     1561 actttaggag tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg
     1621 aatcctctcg tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc
     1681 tataaagact ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg
     1741 gcacttgcca tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta
     1801 atggctgcga ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac
     1861 ttcttctctc ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct
     1921 atttctgtcg atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt
     1981 gtctctacag gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc
     2041 gaaagtccag ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca
     2101 aaagcaatcg agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac
     2161 ggaatattga ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga
     2221 tgcgaaaacc actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga
     2281 aagacgtgta tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga
     2341 ttctcctcct agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag
     2401 ttgttcagct ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc
     2461 agcagtttag cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact
     2521 tttatgatac gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta
     2581 cgctgaaatt ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat
     2641 ttgcgcaaaa gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac
     2701 agcaactgca aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag
     2761 aagaaatcat tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct
     2821 ggccatttga aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt
     2881 tttttaatgt tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct
     2941 tttttttaag aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga
     3001 tttctgacaa tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt
     3061 attttcaagt agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat
     3121 attatagtga tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac
     3181 cgccctttac agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg
     3241 ggctgccagc agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt
     3301 tcgcctatct ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta
     3361 atcatttagt tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta
     3421 gacacaaaca tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc
     3481 tgactgtgtt taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt
     3541 taatgacaga tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt
     3601 tccgtaatga gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc
     3661 ttctgatttc gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg
     3721 tgactgaaga actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc
     3781 aaaaacaaaa actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa
     3841 ttttaccaga attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac
     3901 tttatgttgt tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat
     3961 tgatcaaaac aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag
     4021 cagatgattg ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt
     4081 atttatcaca ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca
     4141 agatgctttt gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg
     4201 atttcctgaa acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca
     4261 agcggtatcg aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg
     4321 gatattggat gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta
     4381 tgagacgaat gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg
     4441 ccaaaaaagc ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg
     4501 tgggatcgct tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct
     4561 ggattacgca ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc
     4621 aatgaatgcg gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac
     4681 accagtcaag cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc
     4741 tcctcaagca agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa
     4801 ataaccttgt gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg
     4861 atgataaaaa aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa
     4921 ttctttttaa taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa
     4981 acgatattca aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag
     5041 ctatttttat ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc
     5101 gacacttcag ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt
     5161 atcttgcaaa ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga
     5221 caacgacaga tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg
     5281 ttttgtctgt ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc
     5341 tgtccttgta taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata
     5401 ttgcttctcc tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat
     5461 tgcttatcaa ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga
     5521 cagcatgcgt atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc
     5581 aactaccttt tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg
     5641 tcggtgggat aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg
     5701 tttaatttaa aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct
     5761 tttttgatta atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat
     5821 aagctgctcc agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa
     5881 atgcagttgt aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg
     5941 cagcaagaga gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt
     6001 cgatcatcca accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg
     6061 gcccaaataa aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc
     6121 caagtaaaag ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg
     6181 ttaatccaat agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct
     6241 gagaaaggac agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc
     6301 ggttcataac gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa
     6361 acaaagtgat tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc
     6421 ggtctttttc aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga
     6481 gcttcgtcga tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc
     6541 aatttttcag cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct
     6601 tttactgtta cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg
     6661 tggtatgaag ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat
     6721 tgacgtgctt gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt
     6781 tgttctagta agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt
     6841 acgaacaagt tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt
     6901 tcagttaatt gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct
     6961 ggtgcgtaag ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga
     7021 cgagaaattt tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat
     7081 aagtttttgg agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat
     7141 cttcaccttt gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat
     7201 gagttattat ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca
     7261 cgaatgtcaa gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa
     7321 tgagagggta tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc
     7381 cggtatcacc attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg
     7441 aaaacagctt ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag
     7501 gaacggctgt ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa
     7561 agattttata cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg
     7621 aattaggaac atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt
     7681 taagtaaggc agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat
     7741 tatgtggatt tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta
     7801 tgttagaatc aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga
     7861 tgtttgcgaa tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg
     7921 tcggaaaagt gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac
     7981 acgtgatagc gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg
     8041 atggaatgac agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata
     8101 ttggtggcgg tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct
     8161 atgtggacta aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa
     8221 aaaatgagga aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg
     8281 gagtacctaa gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt
     8341 tatctatttc tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt
     8401 atcagtatgt agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact
     8461 tagtcgtatt gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga
     8521 gaaaagtata cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat
     8581 tgtttgaaaa aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt
     8641 ataaacagca aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa
     8701 aggaattctt tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg
     8761 ccgattgggt aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa
     8821 gtaagtattt ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat
     8881 attgatggaa cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg
     8941 gaaaaattac gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat
     9001 gcgcaagaga tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc
     9061 ggttttttag accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg
     9121 ttcgttcaag aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc
     9181 aagcgttctt cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct
     9241 gtcttgcctg aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca
     9301 tttttcgatc aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc
     9361 tggcatgaga acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg
     9421 cacttagcag aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa
     9481 aacgaccgtg aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca
     9541 gaagtacaag cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag
     9601 gcattgaaag agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt
     9661 aataagccca gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc
     9721 gcagatgcaa gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc
     9781 aagaaataag aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat
     9841 ttctgaagaa gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg
     9901 tcatagtccc atttttttat tttatttttc aaaactaaga aataccagga aagagttcct
     9961 tttgtttgac cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag
    10021 aagaggaatt tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag
    10081 agatgattac tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg
    10141 taaatggtga gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt
    10201 tgcaagacgg cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct
    10261 ttgagtttgt aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc
    10321 ctggacaagg gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc
    10381 aggctcgttc ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag
    10441 aagaagccag aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg
    10501 aaaaacaagc aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata
    10561 cacacgttca gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg
    10621 ttgggatgac aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa
    10681 agtttctgac tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa
    10741 gcggctgtat cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc
    10801 agatcaacga tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag
    10861 ccggaaattc aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc
    10921 attcgctaca aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga
    10981 gaaaaaatca aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca
    11041 gcagaaaaag aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg
    11101 cttgttgtag cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca
    11161 actatgatac aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac
    11221 taaaaatact cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt
    11281 tttgttctat cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc
    11341 acagcttttg gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat
    11401 gtgcggtgta cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta
    11461 taaagtagcg atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg
    11521 cgaagtgatc cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa
    11581 agacaacaat tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc
    11641 cgatttaagt acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa
    11701 tgaagcctct gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct
    11761 tcgtgaacag ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga
    11821 aaacgcggaa tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac
    11881 cggcaaacta ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa
    11941 agcagtagaa taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct
    12001 agaattaagc caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga
    12061 tgaaacaaaa acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat
    12121 ccaggcaaaa caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt
    12181 tgaacgtatc gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg
    12241 tcgagtagct ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga
    12301 acaagtgcca atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct
    12361 gctttcagaa atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga
    12421 tgatccgccg ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct
    12481 cgatatgtac cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa
    12541 agagcgtcaa gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta
    12601 ttatatagaa attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa
    12661 acaaacttta gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca
    12721 aatcctcgaa gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag
    12781 agaagaagtc aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt
    12841 agatgtgcta caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact
    12901 tgtcagtgac aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt
    12961 attaggacat caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt
    13021 gctgattaca ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac
    13081 tgttctgatg gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt
    13141 tgatcggatt tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt
    13201 tatggtagag atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat
    13261 tttgtttgac gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc
    13321 aatcatcgaa tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca
    13381 tgaactgacg gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt
    13441 tgaaaaagac ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag
    13501 ctacggcata catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc
    13561 agatatcctg catacgctag aaaatggcga taacggacac catactgttc cagctgaagt
    13621 agaagagaaa actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa
    13681 tcaagaccat atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga
    13741 attatccgaa aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat
    13801 gacgccaatg gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg
    13861 cggatatcga tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg
    13921 ggagaagtag tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac
    13981 gccggcagta ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg
    14041 attgataacg gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg
    14101 acaagtaaga tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg
    14161 gaagctttgc caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa
    14221 gaaaaacaag gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca
    14281 ccactgcgtc aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc
    14341 ttgaaatacg tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga
    14401 ctggcattga gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg
    14461 gcaactgctg gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc
    14521 gcaaaaaaaa tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt
    14581 tcattgcctg aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt
    14641 tttatcaaga acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg
    14701 gtcggtcgtt tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta
    14761 aatgttcatc cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta
    14821 attagtaacg ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat
    14881 aatctacggt ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat
    14941 ttgtcctacg cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga
    15001 gagacaggta cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat
    15061 aagtctgtgg aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca
    15121 gaaaaaacag cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa
    15181 acagtggaaa aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat
    15241 gaagaacaag cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca
    15301 ctccatcctg aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag
    15361 aaattagaaa cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg
    15421 cacggaacgt atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct
    15481 gcccaagagc gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat
    15541 ctgcaagaat tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta
    15601 aaagagcaaa aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac
    15661 agctttattg tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt
    15721 cgggagatga tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa
    15781 gcaacagcaa tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa
    15841 gcacaagcac gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc
    15901 catggccgtc cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga
    15961 atccaagacc cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc
    16021 caatcaccaa gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct
    16081 gctgatatcg atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg
    16141 gctgaagaaa aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt
    16201 gccagtgaca cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct
    16261 gaggctgaat tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt
    16321 gttttaaaaa caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat
    16381 cctttgacag acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca
    16441 ggggcatacg gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat
    16501 tacagtatcg taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa
    16561 acaaaaaagc ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt
    16621 gaaaagatta cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc
    16681 cgggcgaaca agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa
    16741 agcaacaata atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc
    16801 aatcagggtc tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc
    16861 ctttagttca acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag
    16921 aataccaatg gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata
    16981 gactttgccg tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac
    17041 tgggtcattt acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc
    17101 ttttgttgca actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt
    17161 tgcgccattt gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat
    17221 cttattcaaa attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc
    17281 aatgatcatt gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg
    17341 caaaccgcct actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc
    17401 taatccagag ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa
    17461 catagcaggt tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc
    17521 ttctttttta ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat
    17581 atttgataaa gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat
    17641 atcgatggca ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc
    17701 gaatacggca ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga
    17761 gatccagctt ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt
    17821 tggtaacaat gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg
    17881 gaagaagatt tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc
    17941 ttgataacca atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc
    18001 ggctgtggaa gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa
    18061 aacgattcct aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac
    18121 tggaagaaaa tggaaaattg cttcaccggg taaccacaaa aaggcattga caccattcca
    18181 aaacttcgag tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac
    18241 atttctgaat ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat
    18301 ttctgctaaa acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt
    18361 tccttgttct ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt
    18421 agaaacatcg tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac
    18481 gctaggtata tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg
    18541 catacgagtc gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa
    18601 cagcttctcg gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg
    18661 tctatgcaag ttgtttcttc atttactata ctttttttcg gagagaattg caagcgtttt
    18721 attaaaaagt acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa
    18781 aaataggaac attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg
    18841 agtgagggaa aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt
    18901 ttagcgaaag aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt
    18961 tatggtgttt cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat
    19021 attcaaaaaa aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct
    19081 atttctggat tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg
    19141 atcatccata cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg
    19201 gagatcggtg cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt
    19261 atattagaca ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca
    19321 caaggatcga tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa
    19381 aaggagatca cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat
    19441 gacacttatg cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag
    19501 tattctgagt cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga
    19561 aaagtgtaaa tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga
    19621 taggatcaca aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact
    19681 gaaagaaaag ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc
    19741 atttagcggg aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg
    19801 tgaaccgtta ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag
    19861 caaaccaata acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg
    19921 aatagaagtt cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc
    19981 agtagatcaa ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata
    20041 tgaaaagatg gatgaattag gttatgggga attcaaatca cttgtagaat aatggaaata
    20101 cacaaagagc ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg
    20161 attgctattt gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca
    20221 ataaagggaa cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag
    20281 gtgtggttac ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc
    20341 agatcgcact aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt
    20401 atgtccatca ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag
    20461 aaaagcaact gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg
    20521 caatcatggc aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca
    20581 cttatttgac gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc
    20641 taaaaggaaa attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa
    20701 acacaaaaga cgctacagct ctatctgaag caatggaagc tctctcggct cttggttaca
    20761 gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg
    20821 atgagtatct acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga
    20881 cagatgaata cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc
    20941 ttcgaccaca gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga
    21001 tttacatcga agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc
    21061 ctcctggatt aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata
    21121 ttcgtacgac gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg
    21181 aactggaagc aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag
    21241 aagagatgct ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc
    21301 caaccgcaca tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac
    21361 gggcaggtat gctttctgca cctttacgag atcgatttgg catcatttct catatggaat
    21421 attatgacga acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag
    21481 agatcattga agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg
    21541 ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg
    21601 atcgtcctat agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt
    21661 atgttgacca gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt
    21721 taagtacgct ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac
    21781 cgtatctgat ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt
    21841 atgcctatga acattttggt tacgactatc atatatgatt tttgctttta gattggagga
    21901 aaatgaatga gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg
    21961 tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc
    22021 aatcgacaaa cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat
    22081 tgtgaggatg cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag
    22141 caagcattga aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg
    22201 ctttctgacc cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc
    22261 aagatttttg aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat
    22321 gctcgttttt tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat
    22381 gaagaaacac cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta
    22441 tattcttatc gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg
    22501 ttggaaaagc aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt
    22561 ttgggaaata tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta
    22621 gaaggaaggg aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga
    22681 aagacaccgc accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat
    22741 atggttgctc ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg
    22801 gatcaagaaa atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc
    22861 catttgtttt taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat
    22921 accaataact ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat
    22981 atttggattg atgaaaataa aataacaaac aatttgtaaa gttaacgctt tctttttaag
    23041 gcgatgttag agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa
    23101 gtaacacttt cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa
    23161 tgaagaagct tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa
    23221 atgacactga agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc
    23281 aaaaagctgc tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta
    23341 gcaagtatga aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga
    23401 tgatccgcct acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa
    23461 aaataacaat cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag
    23521 atagaaaagc acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc
    23581 tttaagctga tatcagtaaa acggaacgtt ttaggatgag tatcatagca tgacagcatt
    23641 cggttgatta gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct
    23701 agccggggag ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt
    23761 tggtatgctt atataatagt cattagttgt gaatattttg tgacgaaagt tttcgaaagt
    23821 tgttaagttt ttgagagtat aaaatatttt gtgtaaatga aaaaatccat acaaaaaagg
    23881 aagtcccttc tgtagaataa agttaacgac aaccaattca cagaaaagag gacttcccta
    23941 tgaatgattt tactacagaa attgtgcaaa ctctagtcac taaaggcgat ttaaatgaat
    24001 tattccgttc gcacttagaa aaagcgataa acacactcct acggactgaa ttaacggctt
    24061 ttttagatta cgaaaaatat gatcgcactg gttttaattc aggtaattcg agaaacggtt
    24121 cttactttcg atcaatcaaa accgaatatg gtgaattaac attggaaata cctagagatc
    24181 gtaatggtga gtttaaacaa caaactttac cagcctacaa aagaacaaac gatacattgg
    24241 aaaccactat tatccattta ttcgaaaaag gtgttacgat gtctgaaatt gctgatttga
    24301 tcgaaaaaat gtacggtcat cactatactc cacaaaccat gtccaacatg actaaagttc
    24361 tgactgaaga agtaaatgcc tttaaatcca gagccttaaa tgataagtat gtcgctattt
    24421 ttatggacgc tacttacatt ccactaaaac gtcaaaccgt atccaaagaa gcgatttata
    24481 ttgccattgg tatac
//
LOCUS       DACDPS010000035        24378 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.035, whole genome shotgun sequence.
ACCESSION   DACDPS010000035 DACDPS010000000
VERSION     DACDPS010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.035"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(123..671)
                     /locus_tag="IWJ07_001510"
     CDS             complement(123..671)
                     /locus_tag="IWJ07_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP9333096.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            complement(716..1048)
                     /locus_tag="IWJ07_001511"
     CDS             complement(716..1048)
                     /locus_tag="IWJ07_001511"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333097.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(1062..1292)
                     /locus_tag="IWJ07_001512"
     CDS             complement(1062..1292)
                     /locus_tag="IWJ07_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333098.1"
                     /translation="MCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQKGYPYGPTN
                     LKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(1514..2056)
                     /locus_tag="IWJ07_001513"
     CDS             complement(1514..2056)
                     /locus_tag="IWJ07_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333099.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            complement(2077..3168)
                     /locus_tag="IWJ07_001514"
     CDS             complement(2077..3168)
                     /locus_tag="IWJ07_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP9333100.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEDGGEATGLAVAFNGE
                     YTEGLPIFSEIRGRGSITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYQGAMSIIKSYNLTEYDTFAIKHWNSLPTKK
                     SSLVAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YDWTAYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKMK"
     gene            complement(3176..5386)
                     /locus_tag="IWJ07_001515"
     CDS             complement(3176..5386)
                     /locus_tag="IWJ07_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333101.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYLLLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVINPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQSSKQQGNNQRKPLLNKGIAFVGQSIPLPAGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPKSSVATLSGNRSVQQLGQRSSESKPSISAP
                     KSLQPAFSSLDKSTDQSKKIEKDPKTLPILDAKLGTDFTLPKGRTVQKPLSKKG"
     gene            complement(5402..7930)
                     /locus_tag="IWJ07_001516"
     CDS             complement(5402..7930)
                     /locus_tag="IWJ07_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP9333102.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVLNLIWLDVAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGTSYLALLPIGETPENMRSCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATIIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSFVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVEYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILTHLRLEVNEANLSEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(7983..8393)
                     /locus_tag="IWJ07_001517"
     CDS             complement(7983..8393)
                     /locus_tag="IWJ07_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333103.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVAI
                     FEYLFLRSFISWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYVV
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            complement(8406..8624)
                     /locus_tag="IWJ07_001518"
     CDS             complement(8406..8624)
                     /locus_tag="IWJ07_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333104.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNNPTRVFDAFGKIIEKLFMN"
     gene            complement(8621..9613)
                     /locus_tag="IWJ07_001519"
     CDS             complement(8621..9613)
                     /locus_tag="IWJ07_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9333105.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKGVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKIDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTLVITKKTNQYYVEKLTHNWLDTKGEERK"
     gene            complement(9692..10606)
                     /locus_tag="IWJ07_001520"
     CDS             complement(9692..10606)
                     /locus_tag="IWJ07_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9333106.1"
                     /translation="MIKTTLAVVTTVGITLFSFQLGTLLVNADETVETPKSEIVDTLP
                     GEELPDVETELSGGASETPEQSQPEQPEKELIESEPEFPESTLPTDPIEEEGVHGSTT
                     TDSSTVTTPEQSGTTSSSEKTTDSSSTEQPTMSSTSEESKEEPSQPAEKASEKSEAIP
                     KENKPVVPAKEVTVSVTPSGEITTNTSQGTSVPIVTSNVEELTHIPTPTTPLKVESGQ
                     TIVGVKDGIPLVQDSEGNLVKDLSIPVKKLPSGNIEVKTADGKTKVLPKTGEEIHVVL
                     SVLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(10625..10970)
                     /locus_tag="IWJ07_001521"
                     /pseudo
     CDS             complement(10625..10970)
                     /locus_tag="IWJ07_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10990..11547)
                     /locus_tag="IWJ07_001522"
     CDS             complement(10990..11547)
                     /locus_tag="IWJ07_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333107.1"
                     /translation="MSLEFLGEPIEKEEFVLHYMDLFIQLIKKLCAIDEFYPREQDYL
                     AAERIKISLLWEKFTDENNRPPDYRYLSDCLTNNVLSKEYLFIKREKNQMSVEHFCDC
                     YIALLRSQQLLTVERYGAEFGYVMQAERHRATRCYEKEDQVIDGYEELCIRNNRYLQQ
                     RLYEQLMMDFSNLYRGYECSMIQER"
     gene            complement(11627..12127)
                     /locus_tag="IWJ07_001523"
     CDS             complement(11627..12127)
                     /locus_tag="IWJ07_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333108.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKRELEGQFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            complement(12128..12673)
                     /locus_tag="IWJ07_001524"
     CDS             complement(12128..12673)
                     /locus_tag="IWJ07_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333109.1"
                     /translation="MNSYMTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLESIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLATVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(12670..13926)
                     /locus_tag="IWJ07_001525"
     CDS             complement(12670..13926)
                     /locus_tag="IWJ07_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP9333110.1"
                     /translation="MTPIELRNYRKKLSAIHASGGGKKLLSQKRFAQLLGCSVDYIKK
                     MEQGKRKIPEDFEQRIKERFRLYVESGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDERWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQSYLEWMGTSGGKIA"
     gene            complement(14286..14597)
                     /locus_tag="IWJ07_001526"
     CDS             complement(14286..14597)
                     /locus_tag="IWJ07_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333111.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVGLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            complement(14620..15930)
                     /locus_tag="IWJ07_001527"
     CDS             complement(14620..15930)
                     /locus_tag="IWJ07_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAP9333112.1"
                     /translation="MYLIPGISIVLYLLLVSGLILGLMRWSRLKEGYFASVYWRQLLA
                     RMLIDNRFIYTKKQVGEKQMKEKMRLPKVYFYQKKQELVISFPLDGGRFHERFLKMGN
                     LLSEVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPN
                     LLISGAIGGGKTYLLYSLIKACLDVGTVDICDGKAADLAALGDVDIFKGHVFYKTNEE
                     MIACLRNALKEMNQRYAYMKTMKEPKYEPGKNYAYYGISPHFIFFDEWAAFYGSLDME
                     TRNKVDKLIQPLVLKGRQAGMYVILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYL
                     MTFGEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYD
                     FTVKEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            complement(16099..16476)
                     /locus_tag="IWJ07_001528"
                     /pseudo
     CDS             complement(16099..16476)
                     /locus_tag="IWJ07_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312971.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16545..16997)
                     /locus_tag="IWJ07_001529"
     CDS             complement(16545..16997)
                     /locus_tag="IWJ07_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333113.1"
                     /translation="MGLKFPNNYDITEALDETCVGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNLKQSQEKGLDQKQPSVDSKEKEKK"
     gene            complement(16999..17364)
                     /locus_tag="IWJ07_001530"
     CDS             complement(16999..17364)
                     /locus_tag="IWJ07_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333114.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIEEVL"
     gene            complement(17463..19487)
                     /locus_tag="IWJ07_001531"
     CDS             complement(17463..19487)
                     /locus_tag="IWJ07_001531"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HAP9333115.1"
                     /translation="MVFSITNDTEPIKVIHFANRQARKNVKLFKYEEWNKKKTPLAGV
                     TFTLYNQAGKALGEYITDQNGEILVKGLVANNYYFIENTPLESFQPNTDKQAFTITFK
                     QNAPDDGETLLVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGK
                     EYTISGVLMNKATNTPILQDGKEIRKTLTFIADKVNGTKDLEFVVNASVLKGQTIVVF
                     EKLFRDGIEVAAHANINDENQSVRVNNPDVHTTAVFEDGLTVADPTGLFTLNDMVKIK
                     EIIPGKAYLVKGILMDKATGEALLVHGQPVESAGGFIAKAENETVIVPFTFDLTGLNG
                     REIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKVVNPLMKITI
                     TDAVKYHDLIVGKTYTVNGILMDKSTEKPVLVDDKEITSTLTFVAERIDGTVNLDFVL
                     DARALRGKEVVVFEDLFRDGVLLASHADITDKDQTIKIVNPTITTKATNKNGGKEILA
                     LPNQTVLEWVKVDGLAIGQLYKLLVQGYLTPDGTPFEGTHAEKVFTADKEMMEFLFEF
                     LVDGRNLAGKGLSFVEWLQAKTEDDKEAFEEVVKHNVDLTNKDQTVQFVEAPKPPQPV
                     KPEQPVKAATVVPMLPKTGEQTSSILMVLGLVVLVIVLGITLYKVRKAKD"
     gene            complement(19445..21745)
                     /locus_tag="IWJ07_001532"
     CDS             complement(19445..21745)
                     /locus_tag="IWJ07_001532"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HAP9333116.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTATQPTVEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPVT
                     SQPVEETASITTPASETSTPEGEKAAEATPSVKPESSEKQAEEKQRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIEDQKKKEQDIPKTGTPTLLVTVP
                     TNRSVRVRRATNTAVLGGRKIDWGENIGVTSSPNNPNFTLNYMVRFEINGQVAFCIEP
                     GVSAAKGQVYTATALENYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLLNNFDVRATFHNQTHTIKIGETLKVKSGNDFLQRV
                     KGIQKPKGINVSIHGNEAWITVTKEAPEKAKIHFNQIEVLGTPLAYRRPGAQTIGILN
                     PFEPGDSVVNLTILKQGNIRVFKEDAETSVKAQGASSLNNAVYGLYQADGKKIKEVTL
                     KNVNGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLIDSMNPDNKPKLAGIEISLTSKTTGKVVKTVVTDKDGYATFGKNAV
                     VFDTYILSETKGKEGYKLFEPFEVTISENGQTFHYALEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTFTYGKDRYRLEE
                     IKAPVNYVLNQNHWYSRLPMIQNQSK"
     gene            complement(21903..22130)
                     /locus_tag="IWJ07_001533"
                     /pseudo
     CDS             complement(21903..22130)
                     /locus_tag="IWJ07_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(22220..22492)
                     /locus_tag="IWJ07_001534"
     CDS             complement(22220..22492)
                     /locus_tag="IWJ07_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333117.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            complement(22575..22826)
                     /locus_tag="IWJ07_001535"
     CDS             complement(22575..22826)
                     /locus_tag="IWJ07_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333118.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            complement(23825..>24378)
                     /locus_tag="IWJ07_001536"
     CDS             complement(23825..>24378)
                     /locus_tag="IWJ07_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9333119.1"
                     /translation="DIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVRKNRE
                     ETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDYKNIL
                     PKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENGRDIP
                     NTNRIIQYAYIGNLSLEFLIYGI"
BASE COUNT         7181 a         5287 c         3566 g         8344 t
ORIGIN      
        1 atattcaata ctggtgaaca tgccttagaa acacgaatat agttatggtt taaatagata
       61 ttttctttaa acattgcttg tttaaaagcc atatgctctt gttctaaact tttcataaac
      121 ttttactcta atgcacagct agcagctgct gctgcaattc ctgctacaat atttacctta
      181 gctaactctt tagctgcagc ttttcctaat tgtttttctg caatttttaa taataaatcc
      241 gcagcttttt caaaattaaa agcttttaat gcttcaataa aatctccctg cgtacctata
      301 gcccaagcca ctcctcctcc aggaagagaa ttaatggcta cacatccagc atatcgtttc
      361 caatcaactt tctccattct aggaacaata atatgttcat aaaagactct tgctgctctt
      421 ccttgttcgt tatcttgact gattcgttca cgaatagcac caaaatcctt tatatggtat
      481 acaccatgtt cgtctaaaaa accaactgta tcaaaataaa aagttaattc atctactaat
      541 ttattcttta tttctaaatc aactgtattc actttttctg tggcatacac acttgttcca
      601 gtactaacaa aagtacttaa acctaataag aaaacaccta aaacaccaaa tattttaatt
      661 attcttttca taatttctcc tccataaaaa ataagttact ttttaaatat cttgtttatg
      721 aattcattcc ccaaaggcct tttaagattg atacagcagt ccgttcactt cttttagact
      781 tgtcatacaa gtcttttaaa tgatcccata tcagactagt ttgtctatct agcttcaatc
      841 cattaaacgc taatgaacgg ttcatttttt ctattacttt agataaagct acagaaattt
      901 ttataccttc acaacgcgca tcttggattt caataatagt atcatcaatt agtttttgaa
      961 tttcttgcct atcaacatat tcaatcttag aaagagaatc ttttaaagct actaatgatt
     1021 caacgatttc ttttttctct tcagacattt tcttcctcct tctagtgacc cgtaaagcca
     1081 tgtagagcag agtccattga accagtaatt acactatcta caacgccatt ccaatttact
     1141 ctacattttc catttttcag atttgtcggt ccatacggat agcctttttg acattcaatg
     1201 ttcggattat accagatagg attataccat tcagccgcag aagcagattc agtcgataca
     1261 aacaatccca caccaacaaa tccaatacac atcacactat agccaattag ttttttcatc
     1321 tcgtatccat ctcctaaaaa atagaatagt tcttatagtt attcataaga catatttttt
     1381 ccgtttttgt tttatccttt ctgtcctata acaacttttt tcaagtaaag aatgacatag
     1441 tttttcttaa aaaaacagtc tgtttttttc tatttcttcg gtgtataata aatatgagtg
     1501 ttcatttttt attttaatac gcactccgta cataataaat cttgatcgca tcaatctttt
     1561 gagcttcttg atctactttc aattgaagta atacaaattg agtaacctta ttcattgttt
     1621 cttctccaaa taacaaatat tgatccggat tctctatatc ttgtgtcacc tggtaaagtt
     1681 tcccagttgt tttcatttgt acatgcgggt ctacatcaat actattgtct ttaatacaac
     1741 gatctgtcat ataatccctc acagcttgat tgcgtttata aacatctgtg aaattactcc
     1801 attttctagc aaaatctttt aagaaatttt ttgtcatttc atctatttgc atttgttgcg
     1861 ctgtctcgtc ttctaatgaa ccaatttgac tgttactttc agtactttga agagttccac
     1921 ttgtttcttt tactgcttcg attgtttctt ctgtcgttct caattgtgtt ttttgttttt
     1981 cagaagaaaa ccaatgacaa ccagtaagac ctatcaaaca taaccaacta aatactaaaa
     2041 ttatcccttt tttcacgttt ctagtcactg cctttcttat ttcatcttac gaaccagact
     2101 acgaattttt gtttgattgt acgtgcgttg ataccgagcc ataatccgtc cttgctcggc
     2161 attctgttcc acactagaga aatgttgtcc gccatatgaa acatctgtta taattccggt
     2221 gtgaccatag attccaggag acaatgcacc acctgcttgc caattcacca catctcctgc
     2281 ttgtaaatca cttggttttg ggttcatgat cacttgccaa ccataggctg tccaatcata
     2341 atcttcccct attaacatcg caaacatgtg tccagaaccc attaactgtg ggccacctag
     2401 ttgggataca taccacgccg tgccaccata acactctcca ttatttaccg atcgtccaat
     2461 gatccgatct aaaactttcg cattaccttt acttttccct gcaacaagtg acgatttttt
     2521 tgttggtagg ctattccaat gtttgatcgc aaaagtatca tattctgtta agttataaga
     2581 ctttatgata ctcatcgctc cttggtaata cggactgcct tcggtaatac ctgccgccgc
     2641 atagcctcca cgacctagaa ttaataaaca actcccagga tctttattgc ccacacattg
     2701 attaaagttg ctttgactcg ccaccataaa gccgtaatag tcataacaag cttttagatt
     2761 cggaaacttc atatagaagc cgccttcaga accgccaatc ccccgtgcag aaccttgtgg
     2821 atagggacag cctggaaacc aggtaatccc aaaaaagttc gtatcgtttc ttccagattg
     2881 tgactgtcca taagccgatt cataacccaa ttgagaaaga atcacactcg gcaacaactg
     2941 atactttacc gccgcaccaa ctgctgcttg cgcatgttca tctgtgatcg aaccacgtcc
     3001 tctaatctct gaaaaaatcg gcaatccttc ggtatattcc ccattaaaag cgacagctag
     3061 acctgtagcc tctcctccat cctcggaaag ttctcctgaa aaaaagaatg gcaatagaaa
     3121 aaagggaaga ataaagaaaa gaagacaacc tagacaccct ttcttcattt cttcctcatc
     3181 ctttcttcga caatggtttt tggactgttc gtccttttgg taaagtaaaa tcggttccta
     3241 gttttgcatc caaaatcggc agtgttttag gatccttttc tatttttttc gattgatctg
     3301 ttgacttatc taaagaagaa aatgcaggtt gtaacgattt tggtgcggaa atcgacggtt
     3361 tactttcaga cgaacgttgt cccagttgtt gcacagaacg atttcctgat agagtggcta
     3421 ccgacgactt aggaacagag tattgattca ataactgctg cattgctgga taactcccat
     3481 ttttcgcctg atttttcact tggcgaatct gttgggaaag agaaccagca gctgttaatt
     3541 gtggcacggg tacttttcca gcaggcaacg gaatcgattg accaacaaaa gcgatacctt
     3601 tatttaacaa tggttttctt tgattattcc cttgttgctt actcgattgt gccaacgatt
     3661 gtacctgttg ttttggtctt actgaattca tggacgaatt tggaatcaaa ccatttgaaa
     3721 gaccagaatt ccactctgtt ttcgatttgg tagaagacat ttcaggctct accccatgat
     3781 aacgaacatg acgaccacta taaggcaagg cttgatttga acgcccacct agttcaaagg
     3841 gattaatcac ttgcctccct tttaccgatt cttgtggtgt acgtttttgt tgaaactttt
     3901 gaaatccatg tatcgctgca gaagcgagtg gattcgttgc tcgatacaca ttccctgcta
     3961 atcctgcacc tttgattgcc aaattttgtc cagcagttga tagttgacca ggcagttgtt
     4021 tcatttgaga caaagaatga cttgctatgt ttttcgtgtt gtttatagtt tgatctgcag
     4081 ctcctaaccc tagtttcttt aataaatctt tccgttttac aatgagtaaa attgatagga
     4141 gtgctttgac aaacatattt cctaaaaaac caaaaacagt attcattgga attaaatcgt
     4201 ctactatgtt atagatcaaa aatgccacca aaataatcac actgtaaaaa gatttttgaa
     4261 acatcaagcc caaaaaggac tttaaggtat tcatgagcat ataatcaaat actggaacaa
     4321 aagaagcgag taatgaaaaa ggaatcacaa gtaataaaca caacaccaac atttgaaaga
     4381 taaaacgaat catgccaatc actacaagtg gtaatccaat acttagatta agcaatggca
     4441 ccatagaacc aacgactgct ttataaacag ctttagttgg ttgaatatag acacgataag
     4501 ccttcttttt ccctctatct tctcctgcag cagtggtcac ttttgtctca atatcagata
     4561 attcttcact tgacgcttcc ttggctagaa attcttgtgg ttcaatctct gcttgataca
     4621 attcttctaa atttgttgtc ccataattta acaatagata cggatcaaca acagccgctt
     4681 tataataaag ttggcgcact ttttgaatgg cttccttttg tgtcgttggc actaaaacac
     4741 cattgacatc ttttgtcgcc aattcatcta gactatttgt agcagaaaaa ataaagcctt
     4801 ctacctccac ggacaaatca tttaaccctt tggtaaaagc ttctccttta ctaggtacgt
     4861 tactaccata gccaaaccaa accattgaaa aaatgacaac gaagagaaat cgaataaaca
     4921 ccatttttgc gtaatgtttc cctctaaata tacgaagagt aaatagatat aacacataca
     4981 agacgacaat ccccgcacca ataggatcgt atatttttga ataaatagta tccgaaaaag
     5041 tgaaaaactg gtgaattaac tcgttcattg cagctccttc atagagcttc tcaatgacaa
     5101 aatcaaggcc ttgccaaatc aatttactta aactaaagaa catattcgtc atcattttta
     5161 gaaaatctgt gccatttgcc ccaaataaca ttttgcctaa tgaaaattca ctatcagata
     5221 aataagaaga aaaggtatca atatgaaatt tcccaatctc atagatcgat tgatccacct
     5281 gcttttctgc cgcatacacc aatccaccaa caaaaagcag tccgaatcca atcgttaaaa
     5341 aaaacaaaac gcaccatttc ttttccttat gctttatata agccattgtt tcacctcatt
     5401 tctattgcat gattaacgta tcctgttctt ctacttgaga gtgacttgtt ttttccgttg
     5461 tttgtaatgc agcggtcatt tcttcaaaca aactgtgtac attgagtttt cctacacgtc
     5521 catagggatc taacatccaa cactgtcctt taatcatatg gctcatttca gataagttcg
     5581 cctcatttac ttccaaacgt aaatgagtca aaatcttttc tcgctcactg tcttcatcaa
     5641 aagcgaagac aacaccaaaa ttcccttttt ctttatctga acctaagtcg ccaataaatt
     5701 gtgtatctaa cactaattga ttcatctgtg aacgccccac acgcgccatt gctgcaataa
     5761 tactacgtcc tgcgttcgaa ttattaaata cccatgcttc tgtaaaataa atcgctgttt
     5821 tcttactact atctcgtttt ccaaacattt cacaaaaacg acctaaacta atcatcaaac
     5881 acaaagactt tcgatccgca tctgtatata gttctggtcg gacattgtca tttggtaatt
     5941 tcaaatcttg gatctctaaa atcgttgttt tggcattaaa gtctaatcct gggttttctc
     6001 cataagaaaa tcctaaacgt aaaatcgaat cttctaccgt caagcgtaaa aactcaccat
     6061 attctcgaac ctctttttgt tccatttgtt ctaaatactc gaccacatgc atcaagccaa
     6121 cttgttgccc ggcttctttt tgaactaaaa cttcttggat cccctttaat accgcccctt
     6181 ttaaaaggtg attggtacct aacggactta actcatcaat catatcctgg gctacttgtt
     6241 tcgcatctac tcgggataaa caaacaattg gatctaatac gccataatta cggggatttg
     6301 tagcatctaa cgtgacaaaa ctcatttttt gcaaatgttc cacaaacaaa gggtaatgtt
     6361 tctgataata cgggtcttga atgagtttcg tgaaccattt gcgcttttct cctttgggat
     6421 ctacaaataa actttgtaca ttcataaacg aactatacaa aaagagcaag ccaactaaaa
     6481 atgttttccc tttaccagtt tttccagtta ctgctacatg aggcgcatca aacaacgcac
     6541 ctttgattcc ttccgcaatg gctaaaagat ttaaaaagac aaagcgattt gacgcgtaga
     6601 tcaattcttc taacggtgtt ttctctcctc tagaaccacc gtcccctaat gtatcttgac
     6661 gaccaatata ccagccagat tccataccta actctgatac tgctgaaaac aaccattcac
     6721 caactgcttc tacagttgtt cggtgcttcc aatgtaactg ccgtcccaat ggttgtccta
     6781 ataaacattt atagaacaat tctcgttgat cattttttgc acggtggata tggataatcg
     6841 tatgaagctg ttgaataatc gttgcactat tttgacgaac ttcttcaacc gtttgaccgt
     6901 atacaataaa acatgcgatc caatcgacga caggttgccc attatctact cgttgcttta
     6961 aatgttttac taatccccga acagttaata agcgttcact aggttcttca tcattttgaa
     7021 ccaattcctt atgttctgtt ttgaaccgtt ccgatagcca attcagtttt aaaccaatac
     7081 ctccctgtcc tttcaagcgt ggatagtgaa ttttcattcg taattctgct gggaaagaaa
     7141 gatcctgtgc aaattgaaat agatcacaac ttctcatatt ttcaggtgtt tctccaatag
     7201 gtaacaacgc taaataacta gtccccaatt catcttctaa ttgaagaagg cctttatgtt
     7261 ccttcggtat acgtacatac gttccttgat tgtctttcag tacttcccaa ttattttgtt
     7321 cttctacttt atgagccatt cctcgaatat attgcagacg attgagatac aacagctctt
     7381 tttttactaa gggacgaata gaaaagctat aaagtaattg tgtcacttct tttacataag
     7441 gatcactttc agaaaacaaa gtttccaaat catctacttc taatccaaat aagcgtgcta
     7501 aacttcctgc tacatctaac cagattagat ttaatacatc atgaaagctc tctaccgttg
     7561 ctttttcttt gactttgatt cctaaaataa atgaaggacg atagacttgc ccttctcgtt
     7621 ctaacaatgt cactgtttct tttgcataat acgcaccaat ggcttgacta tcaggtgaaa
     7681 atcccttacc taaggtgtca aactttgttc gtaaattcat tgttcgtgga tacatcacca
     7741 actccacatc ttcataatct gctaaaagca acagtaaacg agccatcatt tcttttattt
     7801 cctgtgtctt ttttggtgtg ttttttgaaa ttgttcgtgg aaaaacatga tagtaggccc
     7861 atatacaatc ctcgccattt aacaaacaat ttttcttaaa tgcttttact ggttctttta
     7921 caagcaccaa taagtttcac ctcccaataa aaaaaggaac aaccagttga ttgtcccttt
     7981 ttctaatcct ctaaataaac atacttattc acaaaaagta attcttccaa tgcattgttt
     8041 gataccttgt cccagttata atacacacaa tcaccattca tcatacgcac tacataacaa
     8101 aaccaatccc ataaataaaa atgcactttt tttccatcag gttttagttt tgccagtgct
     8161 aacacacata aatagggtag ccctatataa aaaacaactc ctgctggtgg aaaaaaagaa
     8221 ataacccaac taataaacga acgtaaaaat aaatattcaa agattgcgac caacaaaaag
     8281 gttgctgcat tgactaatgc aactggaaat ggaagacgcc aattgcttgt aatctgttgt
     8341 acgcgataag gttccgcata aacaattgaa taatcataag gttcccaatc catgtttcta
     8401 ttctcctaat tcataaataa tttctcaatg attttaccaa acgcatcaaa tacacgtgtt
     8461 ggattattta aaaagaaata gactgctgca cctgctgcaa tagatattaa tgcatttcct
     8521 acttttgatt tcataaaatt tcgtaacccc agataaacaa ccaccattat taaaaagtag
     8581 ccaccttgcg ttaatataaa atctacaatt cccttcagtg tcatttccgt tcttctcctt
     8641 tcgtatccaa ccaattatgt gttagctttt caacataata ctgatttgtt tttttcgtaa
     8701 taacaagcgt catttgttct ttttgtaaaa gtgacgtatc accatctcga aagacgaccg
     8761 ttgcttttac aataaaaggt tttttcgttt ctttataacg caatactttc atctctactt
     8821 gatcaatttt agctgtctga ttgagtcctt caggttcttt catcatgtat gacatatctt
     8881 ttgcattgtt tttcgcatac ttatctaaga aatcttccac aaatccttca acacctttcg
     8941 cttccgacgc atcaaccgtt tcgtataatg tgtcatcata agtaatcatt cgttgttttt
     9001 ctgtattctt tggtagtgcc gtaaaataag gataagcaat aatacgacag cccttgtttg
     9061 aaatagaaaa gggaagattg agtacttgtg tgattgtcac acttgtttct ttcttactct
     9121 ctggattcat cgagcgaata tcataagtta ctttataact agctgttgga atggtttcaa
     9181 tttcttttaa tccaataaac gaagcagcag ttaattttcg aacacctgaa acacttcctg
     9241 gttcttgaat aaagttttgc gcataaaact ctttttttaa tgtttcttga cgttgttgtt
     9301 cttgttcatg attatcgaat gtattcatat acaaagcaac aaaacgattc ataaaggatt
     9361 caatttcagg tgtgtacgtt aattctgacc gactttgtac ttctttcttt tgtaattgtt
     9421 ggatttcatc tttcaaaaac gacacttgaa ccttcgtttt catggattga aaaaatgcca
     9481 agccccctga aaaacaaaga aataaaacca aacaccaaat ccatttcact gctttttttg
     9541 aatggatttt cttttgcttc ggtactttct ctgtataaac tttttctttc cgccgtagtt
     9601 gtcgtttttt cataagctac tccttctagt atgtttttac tagtacgtac gattgacaac
     9661 gataagcttg tttttctgaa aaatagtcca ttcaattacc ctcattttct attccttttc
     9721 tctttctttt atatccaaca aatccagcaa tacttgtcaa cactcctcct agaacagaaa
     9781 gtacaacatg tatttcttca ccagtctttg gtaagacttt tgtttttcca tctgctgttt
     9841 tgacttcaat attgccactt ggaagtttct ttacaggaat cgacaaatcc tttaccaaat
     9901 tcccttcact atcttggact aatggaattc catcttttac accaacaatt gtttgaccac
     9961 tttctacttt taatggggtg gttggtgttg gaatatgggt caattcctct acattactag
    10021 tgacaatcgg tacactggtt ccttgagaag tattcgtcgt aatttcaccg cttggggtta
    10081 ccgatactgt gacttctttt gctggaacaa caggtttatt ttcttttgga attgcttctg
    10141 atttttcgct tgccttttca gcaggttgac taggttcttc ttttgattct tcactggtag
    10201 acgacatcgt tggctgttct gtacttgacg aatcggtagt cttttcagaa gaactagttg
    10261 ttcctgattg ttctggagtt gtgactgtac ttgaatccgt tgttgttgag ccatgcacac
    10321 cttcttcttc gattggatca gtcggtaaag ttgactcagg aaactctggt tccgactcta
    10381 ttagttcttt ttcaggttgt tctggttgag attgttctgg agtttcactt gctcctccac
    10441 ttagttctgt ctcaacatct ggtaactctt cccctggcaa tgtgtctaca atttctgact
    10501 taggcgtttc tacagtttca tctgcattta ccaataacgt tcctaactga aacgaaaata
    10561 gtgtaatccc tactgttgtt acgactgcta atgttgtttt tatcatgatt attcctccaa
    10621 ttttttatgc aattcgtgtt ttgttcatag acgccgcact aatattatct gaacgataaa
    10681 agaactctcg tccgttttct tctaacccaa catatggatt atttttgaat aattgaatca
    10741 atagttcact ggtgttgtat tttggatagt ttgcaaaaaa aataatactg tgcttgtcgc
    10801 ccatctgaaa attcaacaag taattcatag cgataccgct tcaatccttt tcgttgtttt
    10861 aaaggatctc tataccaact tgtccatcgt tccattcgtg gatagagata gacacgatac
    10921 acatagaaca atgccatacc gcataccacc aaaaataata atcgccacat aaaaattccc
    10981 ccttttcttc tacctttctt gaatcatcga acattcatac cctctataga gatttgaaaa
    11041 atccatcatc aattgttcat ataaacgttg ttgcaaatag cgattgtttc gaatacataa
    11101 ttcttcatag ccatcaatca cttgatcttc tttttcatag catcttgtag ctctatgacg
    11161 ctcagcttgc atgacataac caaactctgc accatatcgt tctaccgtta ataactgttg
    11221 actacgcaat aatgcaatat aacaatcaca aaaatgttca accgacattt gatttttctc
    11281 acgtttgata aatagatact ctttggaaag aacattattc gttaaacaat ctgataaata
    11341 gcgataatct ggcggtcggt tattttcatc tgtgaatttt tcccataata aactgatttt
    11401 tattcgttca gcggctagat agtcttgttc gcgcggataa aattcatcaa tcgcacaaag
    11461 cttttttatt aactgaataa acaaatccat ataatgcaaa acgaactctt ccttttcaat
    11521 tggctctcct aaaaattcca gtgacatacc atcttctgct tccttaaaaa ataaaaaagc
    11581 agaaaccgat ttggcttcta cttttctttc cttttatagc tcttttttac ggaagaatcc
    11641 gcaaatgaca ttttttcaaa aaatcaatcg gcacaagttc acgaaaactt ggagcaattt
    11701 tggatagaat cacttcccca tcttgtaaaa tacaatcata aaaatcatca gttacttgat
    11761 acttacgctt cttgcaataa ttgataaaat agtagcgatc attttctatt tccattcgtt
    11821 gttctactaa gcgcatacga acatcatcat agaaataagc cacaaattgc ccttcaagtt
    11881 cacgtttttc aatgggatac tcttctggta acaatctatt ttttcgtcgt ttatacattg
    11941 ctggatacct tagaagcagt tcatctaaac gtctgtcagt atatggcaac tgccgaatca
    12001 acgattctaa tcgttcttta tacagataga ataattggcg caaacaatag tgtactgcct
    12061 ctggattttc taatacagtt ggatcacgtg accaatacgc accaacataa atcataattt
    12121 ttttcattta gacaatcaca ccagcaatac taattaccga tccacaatag gtagaacaag
    12181 tggcatagcg accaatttcc tctaattcta tctcaatctc ttctaaagaa agatatgtct
    12241 gttcttctgt tggctggcga ctcatttccc aagtgacagt cgccaatgaa atttcttcca
    12301 taagaggttt ttcgctgcta agtgctgcca cacagttttc gtatatcttc attttatcgc
    12361 agcctttttt aaccgctaaa atcgcaggta ttggcaatga aaatcgataa aagcgaatgg
    12421 attctagcct acctttcctt cggctaaatt ccaactcttc gagttcttgt tcgtatccct
    12481 tacgaacttc taataagcgt gcaatttctt gccagttctc tttttcctga taacgtccag
    12541 cttttaccgc ttgttcaata taaggaactc gaaaacgaaa ataagacaaa tcaaccatct
    12601 tttcatttaa tgcttcgatc gcagctttta aatccaacag gcgcaaccat tctgttgtca
    12661 tatatgagtt catgcaattt tccctcctga cgttcccatc cattctagat agctttgttg
    12721 ctcgtcattt agttcagcat tatcaatgcc ttcttgtaca aagtctcttc ctaaaatacg
    12781 atctgcttct tgtaacaatt ttaatgtcgg agctacttgt ttcaaaaacc agcgttctga
    12841 acgatctaat gagaattttt ctggctttac tgtaaaatcc aattcttcca tcgaacgaaa
    12901 caattttgcc catcgttcat caacaatccg tgcatcactt tccagtcgat cccaaacaat
    12961 aagctttgca ttaattaaac caacgccaat ctctcctaaa cgtttcggtg aatcacctaa
    13021 aataaatgct tctactgctt gtgttgcttt ctcattggct agccgtaatt catatcgatt
    13081 ataaatccca tatttcgtta atgcttctaa ttctgaaatt ttttctcggt tcgcaatttc
    13141 aaaacgtttt tcatagaaat tgaaaaacaa tggactcttt cgcgatccaa aatacaagga
    13201 taatccttca cttcgaaaat agccatcatc taaaaaacaa ccgccaatat tctggaatct
    13261 cttaaaatcc atttctaata gacccgcttt ttgtttttct agtaattcaa agagatcgaa
    13321 atgttcttcc gtttccaatc cccaaagctc atctaacgca atatcgaatc gactacattg
    13381 cacacgtgta taaccagctt ccaactttcc ttgatttttc caaatagaat atagccatgt
    13441 tcgccaatct gtttgttgtt cacgcatgac ttcctcatat tcccggcaac cttgtcctga
    13501 aaattgtaca agtacatttt ctgttacggt tcgttttgaa tgccagaaca cccagatatt
    13561 cccacgaaca gaaagacagt caaaaagata caagcccgtt ggacgtgttt caaattctgt
    13621 cagatccatt cgtaatacac gttgaatcac tgttttgtag tctgtcccaa aaaaggaaag
    13681 acgcaaataa tcaatcattg ctcgaagatc tattccgctt tccacataca atcgaaagcg
    13741 ttccttgatt cgttgttcaa aatcctctgg gattttcctt tttccctgtt ccatcttctt
    13801 tatataatct actgagcatc ccaataactg tgcaaaacgt ttttgagaga gtaacttctt
    13861 ccctccacct gaagcgtgaa tagcactcaa tttttttcga taatttcgca attcaattgg
    13921 cgtcatattc tacctccttt ataaaataac aaatttcgat tttttcaatc tgctagaaat
    13981 gccggtatca taggtggtac cccctgattt ttctaaaaag gggtacttct tctaacttta
    14041 gctaaagcct tgatataact gctttaaata tactttttta tttgtgttat gttatttctg
    14101 ccccccatat tatatatcgg ggggctttac acaaagaggc cgtgcgcaag cgcacgacct
    14161 ctttgtgagc tgcacacgct gctagcgagt gcgcattata ggcccatgct tcgcagcctt
    14221 aatgcgcacc gccaggcaga gtgcggctac cagactacgt ctagagccct cccgatagat
    14281 aataactaca aaattaaatg atctaatttt gttatttcac tatatttttc aggagaaaaa
    14341 acgcgataaa ttgacaaagt taaatcctct tctaaaccta ctcgataatg atacaatcga
    14401 gtcaatcgtt ctatatcttt tttcgttagc aatacttgat taataacttt tcctgtaaaa
    14461 acacgttctt tgcgcaaaaa gcggtaatca gttacttgtt caatacgatt caaccaatca
    14521 taaaacgttc tggaagaaag agaaggatta atcattcgta ttgctgtagc tactcgataa
    14581 taacaactct ctgccattta acttcacctc cttagttgat tacaaaatat gcgcatcaga
    14641 aaaacttgtt ccttttggat cttttgctaa atacatttgg cgctctagtt gttcttgtaa
    14701 caactgatta tcatctccca tcgtttgatc ttgactatcc tcttttactg taaaatcata
    14761 gacaatcatt tcagtaaatt tctcaaattc tttgataaag tgatagtctt tgtaaacaaa
    14821 tggcgcaaag aactctacag gtacatcaaa tcctggaaaa gcatacccac gaccaggttg
    14881 atctttgttg taaaatggct tatttttaaa atcttcacca aaagtcatta aatagccaat
    14941 cggagataat cgaccaacag aaatacggaa cgttaaatta tctcgaacac cacttggaaa
    15001 atattccgca tctggtcgtt gcatcgctaa aataacgtac atgcctgctt gacgaccttt
    15061 taatactaat ggctgaatca atttatcgac tttatttctt gtttccatat ccaacgaacc
    15121 ataaaaagct gcccattcat caaaaaagat aaaatgcggc gatatcccat aataagcata
    15181 atttttccct ggttcatact taggttcttt catcgttttc atataggcat atcgttgatt
    15241 catttctttt aacgcatttc gtaagcaagc aatcatttct tcattcgttt tgtaaaacac
    15301 atgtcctttg aatatatcaa cgtcacctaa agccgctaaa tctgccgctt ttccatcgca
    15361 aatatctact gtcccaacat ctaaacaagc tttgatcaat gaatacaata aataggtttt
    15421 tccgccacca atcgcaccag aaattaacag atttggcgat tgatcatata cccaatccaa
    15481 ggttttcatt aaatgaatac gtccattttc tgctatacat tgatcaatag gaatccgacc
    15541 agaatcggaa agaaatacat aattgattcg ggctttttct ctttcttcac gaattaaatc
    15601 acgcaaaaat acttcagata acaaattacc catttttaaa aatcgttcgt ggaaacgccc
    15661 accatctaaa ggaaaagaaa tcactaattc ttgtttcttt tgataaaaat aaacttttgg
    15721 taatctcatt ttttccttca tttgtttctc accaacttgc ttctttgtat aaataaaccg
    15781 attatcaatt aacattcgag caagtaattg ccgccaataa acagaggcaa aatatccttc
    15841 ttttaatcgc gaccaacgca tcaaacctaa aattaatcca ctaaccagca ataaatacaa
    15901 cacaatactt attccaggaa taagatacat ttgccaattc gaatacgtac ttaaacgcgg
    15961 ataaaatact ttccaactaa accaacctaa cagaatcaaa aatgggagta aaaagaaacc
    16021 ataataacag aaccacaaat aacgattgaa ataacgaatt cgccgaccac gatacttaaa
    16081 aaaatgaggt tccaattgtc acattcctcc tttctgttta tacttctcct gtgctaaaaa
    16141 ctttcgacaa aaagcaacta atactggaat atcacattta ataaaaaacc ataattgaaa
    16201 acgatctaat cgattttttt atgggtgtgc acacatatta catattcgta ctacacgaat
    16261 ccaaacctct tcctgatgat tttgtttcca ctgatatggt aatgaaaaac gaacaagatc
    16321 acgtaaaaac atacactgcc caataaccgc acaaatatat gcatttgttt gtgcaaaaat
    16381 cgtcggattg tctccaaaat actcaatcaa ttgttgggta ttttgacaac aagccacaat
    16441 ctctcgaagt gtttgattgg ttttatatga attcaaaatc tccccttcct tctaccttag
    16501 ataaaaagaa taggaagact tcttcctact ctttcaaaaa atccttattt tttctctttt
    16561 tccttagaat caacacttgg ttgtttttga tctaaacctt tttcttgtga ttgtttcaga
    16621 ttaggattat ttgttggagc ttctactttc tctaatcctt tcgcataaat cactaaatct
    16681 gaatattcac gattgccatc ttgtgctgga atatatacca catgatattc tggatcaatt
    16741 aatttcacag gatcacgtcg tttaaaacca gtcaaatcta catctgctgg aaatttaact
    16801 cgtcgtgcaa tctttggttc atctggattt ccaaaaacta attgtgctcg ctgtgatcga
    16861 atttctcccg tataatcgcc tgtttcatta tcaaatacac gatcagactc ttctaatgcg
    16921 ccaaagcgta cggctcctac acaagtttca tctaatgctt ctgtaatatc ataattattt
    16981 ggaaatttta atcccatctt acaaaacctc ctcaattttt acgccagtta aaaagacacc
    17041 aacatcttga tagccagttg ttcgacgctc tgtttgtccc atatcatttg tttcacgact
    17101 attcactcgt tctttacgaa aaacaaaata agcttcttca aatgaaatac gcacctcttt
    17161 ttctggtaat cgtaatcttt tatcctcttc agctgtgaga taaacaaaaa aagcaggctg
    17221 ttcttctcgc gaacattctg cttgtaattg aacatatcgg ccagacacac cattaatttc
    17281 ctttggtgga ttaggttccc gtgaacccaa taaaaatact tgacctaaaa actctttctt
    17341 catacgcaat gctttttctg ccataaagtt tttcctcctc ttaaaaacca aaataaattc
    17401 taaatatttc tacattacaa tcaaacaagc ggttaaaaat cctaaccgct tgttcctcat
    17461 gcctaatctt tggctttacg tactttataa agtgttatac ctaaaacaat gactaatact
    17521 actaatccaa gaaccataag tatactactt gtttgttcgc ctgtttttgg caacattggt
    17581 acaacagtag ctgctttaac cggttgttct ggtttcactg gttgcggtgg ttttggtgcc
    17641 tccacaaatt gaaccgtttg atctttgttt gttaaatcca cgttatgttt tacgacttcc
    17701 tcaaacgctt ctttgtcatc ttctgttttg gcttgaagcc attccacaaa acttaagcct
    17761 tttcctgcaa ggttgcgacc gtccactaag aattcaaata agaattccat catttcttta
    17821 tcagccgtaa agactttttc tgcatgtgta ccttcaaatg gcgtgccgtc tggtgttaag
    17881 tagccttgaa caagcagttt gtacaactga ccaatcgcaa gaccgtctac tttaacccat
    17941 tccaacacgg tttgatttgg taaagccaag atctcttttc caccattttt attggtagct
    18001 ttggttgtaa tggttggatt gacgattttg atggtttgat ctttgtcggt aatatccgca
    18061 tgagaagcta ataatacacc gtctctgaag agatcttcaa agaccacgac ttcttttcct
    18121 cgtaacgcac gtgcgtccaa cacaaagtct agattgactg taccgtcgat acgttctgcc
    18181 acaaacgtta acgtactagt aatttctttg tcgtctacca acactggttt ttccgttgat
    18241 ttgtccatta gaataccgtt tactgtatat gttttcccaa cgattaagtc gtggtatttc
    18301 acagcgtctg taatggtgat tttcattaat ggattaacca ctttcaagcc agttggttta
    18361 tgcgtagccg ttgtacgtac acttgggttc gttactcgta ccgtttgccc tttgtcgttg
    18421 atgtctttgt ggtttgccag ttcttcatca tttctaaaaa gactttcaaa tacgacgatt
    18481 tcacgaccat ttaaacctgt caaatcaaag gtaaatggta cgatcaccgt ttcattttct
    18541 gctttggcaa taaagccacc agcactttct actggttgac catgaacaag taaggcttca
    18601 ccagttgctt tatccattaa gatccctttg accaagtaag cttttcctgg aatgatttct
    18661 ttgatcttaa ccatatcatt taaggtaaac agccccgttg ggtctgcgac cgtcaatcca
    18721 tcttcaaata cagcggttgt atgaacatct ggattgttta ctcgaacact ttgattttca
    18781 tcattgatat tcgcatgcgc cgcaacttca atgccatcac ggaaaagttt ttcaaagaca
    18841 acgatcgttt gcccttttaa gacgctggca ttgaccacaa attcaaggtc ttttgtgcca
    18901 ttgactttgt ctgcaataaa ggtcaaagtt tttctgattt ctttaccatc ttgtaagatc
    18961 ggtgtattcg ttgccttatt catcaatact cctgaaatgg tgtattcttt tccgacaaac
    19021 aggttggtat aagaaacact gtctttaatg acgatcgttt ctaatggatc aaccactttt
    19081 tcaccatcgg ctttatttga cgcttgtgtt tctagttttg gcggaatcaa gtagttacgt
    19141 acagaaacca ataacgtttc gccatcatct ggcgcatttt gtttgaatgt aatagtgaat
    19201 gcttgtttgt ctgtatttgg ttggaaactt tctaatggtg tattttcgat aaagtaatag
    19261 ttattggcaa ctagaccttt cactaaaatc tcaccatttt gatcggtgat atattcacca
    19321 agagctttcc ctgcttggtt atacagagta aaggtgacac cagcaagcgg tgttttcttc
    19381 ttgttccatt cttcatactt gaacaatttc acgtttttcc gtgcttgacg gttcgcaaaa
    19441 tggatcactt tgattggttc tgtatcattg gtaatcgaga ataccaatgg ttttgattca
    19501 aaacgtaatt tacgggcgct ttaatttctt ccaaacgata gcgatctttt ccataagtaa
    19561 atgtaccgtt agttagaaaa aagccttttt cattggtttt aaagatatcc gttgtttctg
    19621 tatcatttgg aatctccatt gtgacataac gattggctaa ggtatcaaaa atcttgaatt
    19681 ctgtattggc aagtggaatc acttttcctg tttcttgatc cactttcacc actttaagac
    19741 gttgttcgat tactttatct tctaaagcat aatggaaagt ttgcccattt tcagaaattg
    19801 tgacttcaaa tggttcaaat agtttatacc cttcttttcc tttggtttca gagagaatat
    19861 acgtatcaaa aacaacggca tttttaccaa aagtagcata gccatcttta tctgtcacaa
    19921 ctgtttttac cacttttcca gttgtttttg acgtcaaaga gatttcaata ccagctaatt
    19981 ttggtttatt atctggattc attgaatcga ttaagtcttt attagcaact ttcactaaat
    20041 caatggtgcc cttaatcact tggtcttttg cggtcgcatt gacagttttt ccaggttcaa
    20101 caacaatttc aattgcttca tcagaaagca cataaccttt tggcgctttg atttctttta
    20161 aaacatattt gccaggatca aggtctttaa tttctgcttg aactttccca ttgacgttct
    20221 tcaaagtaac ttctttgatt ttcttcccat cggcttgata caaaccataa acggcgttgt
    20281 tcaaagaaga ggcaccttgt gctttcacac tagtttctgc atcttcttta aagacacgaa
    20341 tgtttccttg tttcaaaata gtcaaattga caacagagtc accaggttca aaaggattca
    20401 atataccaat tgtttgggca cctggacgac gatacgctaa tggcgtaccc aatacttcaa
    20461 tctgattgaa atgaattttt gccttttctg gtgcttcttt tgtgactgtg atccatgctt
    20521 cattcccatg aatagataca ttgatacctt ttggcttttg aatgcctttt acacgttgta
    20581 aaaagtcatt tccacttttt acttttaacg tttcgccaat ttttatcgta tgtgtttgat
    20641 tatgaaacgt cgctcttaca tcaaaattat taagcagacg ttgaatttct gcttttcgac
    20701 gatcataatc aacacggata tttttgattg ggatttttgt gcccaaagct tcccaaatca
    20761 taaattgtgc cgcaatgtat tgttcatcgg atttgtcttt atttgtgaca tatcctaaat
    20821 aagagatcaa gcttgttttt ttccgtacat ttgaattttt caaatagttt tccaaagcag
    20881 tagctgtata aacttgacct tttgcggctg agacaccagg ctcaatacaa aacgccactt
    20941 gtccattaat ctcaaaacga accatataat ttaaagtgaa gtttggattg ttcggactag
    21001 aagtaacacc aatattttcg ccccaatcaa ttttccgacc acctaaaaca gctgtattgg
    21061 ttgcacggcg aacacgaacc gaacgattgg ttggtactgt tactaacagt gtaggtgtac
    21121 ctgtttttgg aatgtcctgc tcttttttct tttgatcttc aatactagcc gtagattctt
    21181 tgtttggttt cgttggttga ttttgactag ctgcggttga agagattgtt ttttcaacgt
    21241 tttcctcatt ttttccttct ttgtcagcag tagccgctgg ttgttccact gtttcttcta
    21301 ttcgttgctt ttcttccgct tgtttctcag aagactctgg cttcacagaa ggcgttgctt
    21361 cggcagcttt ttctccttct ggagtggacg tttctgaagc aggggttgta attgaggcag
    21421 tttcttcaac tggttgtgaa gtaacaggac tattccctgg gtgttgtgtt tccccaatcg
    21481 tctgatcgag agtcgttttg gaattgaccg ttggcgcttc tgtcacaggg gcctgttcaa
    21541 cagtggcttc accagctggg accccggctt ctgctggttg gacttctgtc acttcagaag
    21601 cgacttgggc aatggcttcc accgtaggtt gtgtagccgt cgtttcttct gcatgtgctt
    21661 gagcagttcc caccaagaaa cagaattgag aaaaggccac caatgcgagt gctttcaacc
    21721 aattcatttt cgtttttgca tacacaagaa ttcctccttt tcctgagaat tttatataat
    21781 aaaacaaaac gccgttacca aaagtgataa cgacgtgaac tgtctcttct atttttatct
    21841 ggcgctctta tctgaacaca tttcatcaat gattttaccc tcccactata attacagaac
    21901 aaaaaagaac ctgcttcgtg gaaatcaact gtccatttcc caattggaat accagcttta
    21961 ttggcaactt caccatcacc aaatctcatg ccataccaat tactaccttc atccttaatt
    22021 tctttccaac catatgacat agcttcatct aacgaatcaa aaatcatgcc actgttgcca
    22081 attgttgcag gatctggctt aagatcttct ggaagagaaa tatcaatcac accataatga
    22141 tccgattcct cagtattatt gtcattacta gctattggtt tggttgttct actggcttat
    22201 tttgatacaa caagtacgct tattcttctt ttacttctcc gttttcaatt tcccattcaa
    22261 accactcagc aaagtcttgc ttaaaaccat ctactcgata cctattttgt aaataggcat
    22321 cttctgacgt cgtaggatct aaataggtag gtttattctc tggacgagca tagacaccta
    22381 cccatgtttc gtaatctcct tcaaaataac tatctaccaa atagcctttt tttctaagaa
    22441 tctcccgaac ttgattttta attattgata cttgttcaaa accatcactc attctatcac
    22501 ctcaaataac tctagattct cataaatatt cccgacaatt tcaatcctat caatgatctc
    22561 taacaaatcg ctacttatgc ttccccattc gtataagaat ttcccttccg caaaataaat
    22621 tttccataaa cttcttcatg atcgtcccaa ccaatatcac tctcgaaaaa ttccacgttg
    22681 ttcttatctt ttagctcagt aaatgccata agttcaacat gttttccaac ctcgaaacaa
    22741 tcacaccacc catcaaccgt tacacgtgtg ccttcaatac ctaagaaact ttgtcctcgt
    22801 gtattaaaca ctaaaacttt ccgcattttt tgctcattaa ttaatcacgc gcgaaatcta
    22861 ggaatcacaa cttattcgct tcttctttat aaataaaaaa actccaataa tcggaaatta
    22921 ctatcttttc ctcattcctt ttttaaatct tcatacttct tcttatcttt gtcctaacct
    22981 aataacacaa attttgaaga aaaactcagt aaaacctgat tcttttttta tcttcacaac
    23041 aaccaacttt ttcttcagat ccttttacta gaacttgtat acaaaaattg ttgaatgctg
    23101 atacaatttg cggttacttg aaaaaacgat agatgtaaaa agataaatag aatcattttt
    23161 tattctatat cagtctatga ttcattgctt atcaatcacc caacccattc gatgccgttt
    23221 tttttaagag aaatttaaga aagaccgtac gaatctcgca ttgtccgatt acttttccag
    23281 aaaagatccc caattctgta taaataaccc tatcttcttg ttggtttcat tttctcatat
    23341 aagaattcag tcaatctacc cactatgacc attactacgt ccaacacaac cgacatatta
    23401 accacgtaag tattccctca cagatctccg aatctccatg gtatggattc ccatcctgac
    23461 gctgcagagg acaacatatt tcatgtggct ctgtaccctt ttgatttgga aacactacaa
    23521 atcaatactc atctataact tatcaagcga actttcgcta ggcttctagt gattactaaa
    23581 ttgttggata acatacctag cccattttat ctagctgtta ttcataacag ttatttcttg
    23641 tattacttct tccgcttgtg ttgccattga gcttgtcaaa aagttactta gaaaaaaagc
    23701 tgccagtaaa gcaactttca accaacactt tttgtttctt atcatatcaa gaaacctcct
    23761 tttataaatt ttttctataa taaaaaaacg tcgataactt aataagtatc gacgtaaact
    23821 atttttatat cccgtaaatc aaaaattcta aagaaagatt tcctatataa gcatattgga
    23881 taattcgatt agtattgggt atatctctac cattctcata cctatctatg gaccattttg
    23941 aaattgaagg agaaaaaagc tttccaaact cctccctttc tagataataa tctttcctaa
    24001 ttttcctcat ccttaatccc atactaaaag aatttatctt tttaaaatcg ctaacccttt
    24061 catcttttgg aagtatattc ttataatctt ctccgtacat aaggtaagga acagtgacct
    24121 taccaagata ggcaatattc attagttttt caatatctgg aatattaata cctttttccc
    24181 atctacttat aacatttttg cctgtataga tggtaaagtt tttaccaaat ttttcaagcg
    24241 tctcttcccg gttctttcgg acttctcgta ttctttttcc cataacaatt ttatcgtctt
    24301 ttgataaact aaatatctca ctatcacgat taatctcctt caaaatttct ttttcattac
    24361 ttaatagaaa aatatcga
//
LOCUS       DACDPS010000036        23698 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.036, whole genome shotgun sequence.
ACCESSION   DACDPS010000036 DACDPS010000000
VERSION     DACDPS010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.036"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="IWJ07_001537"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="IWJ07_001537"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAP9333120.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="IWJ07_001538"
     CDS             complement(1172..1867)
                     /locus_tag="IWJ07_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAP9333121.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="IWJ07_001539"
     CDS             complement(1880..2596)
                     /locus_tag="IWJ07_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAP9333122.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="IWJ07_001540"
     CDS             2926..3534
                     /locus_tag="IWJ07_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAP9333123.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="IWJ07_001541"
     CDS             3859..4182
                     /locus_tag="IWJ07_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP9333124.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="IWJ07_001542"
     CDS             4364..5734
                     /locus_tag="IWJ07_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAP9333125.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="IWJ07_001543"
     CDS             complement(5864..7165)
                     /locus_tag="IWJ07_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP9333126.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9510)
                     /locus_tag="IWJ07_001544"
     CDS             complement(7519..9510)
                     /locus_tag="IWJ07_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAP9333127.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="IWJ07_001545"
     CDS             complement(9654..9920)
                     /locus_tag="IWJ07_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9333128.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="IWJ07_001546"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="IWJ07_001546"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAP9333129.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="IWJ07_001547"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="IWJ07_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAP9333130.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /locus_tag="IWJ07_001548"
     CDS             13264..13827
                     /locus_tag="IWJ07_001548"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP9333131.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="IWJ07_001549"
     CDS             complement(14080..14601)
                     /locus_tag="IWJ07_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAP9333132.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="IWJ07_001550"
     CDS             complement(14604..15446)
                     /locus_tag="IWJ07_001550"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAP9333133.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="IWJ07_001551"
     CDS             complement(15439..16164)
                     /locus_tag="IWJ07_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAP9333134.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="IWJ07_001552"
     CDS             16340..16636
                     /locus_tag="IWJ07_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333135.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17204..18796
                     /locus_tag="IWJ07_001553"
     CDS             17204..18796
                     /locus_tag="IWJ07_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9333136.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="IWJ07_001554"
     CDS             18809..19549
                     /locus_tag="IWJ07_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9333137.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19717..21066
                     /locus_tag="IWJ07_001555"
     CDS             19717..21066
                     /locus_tag="IWJ07_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9333138.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21194..21784)
                     /locus_tag="IWJ07_001556"
     CDS             complement(21194..21784)
                     /locus_tag="IWJ07_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP9333139.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            21931..23283
                     /gene="brnQ"
                     /locus_tag="IWJ07_001557"
     CDS             21931..23283
                     /gene="brnQ"
                     /locus_tag="IWJ07_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP9333140.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6793 a         4772 c         4511 g         7622 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagccattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaaataaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgaaatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttct gaaaattttg gaatcttagt tgtgtttttc agaaaactct cttaatatgg
    19681 aagtaattaa acttttttag ttaggatgat ttaaaaatga atgaagagca agaactgcag
    19741 cgtggactga aaaatagaca tgtccaactg atttctattg gcggagcgat tggaacgggg
    19801 ttgtttttag gttccggaaa gtctatccag ctagctggcc cttcgatatt gctcgcctat
    19861 cttattactg gatgcatctg ttttttcatt atgcgtgcgt taggcgaact tctgttatca
    19921 aacaccaata accattcttt tctggatttt gttgcagaat atcttggcaa aaaagcggct
    19981 ttcatcactg gctggacgta ttggttctgc tgggtatcga ttgcaatggc cgatttaaca
    20041 gccatcggaa tgtatgtacg ctactgggcg cctggtgttc ctcagtggct tcctgaactg
    20101 attgcattag gtcttcttct ttgcttgaat atggtggctg tctcattgtt tggtgagtta
    20161 gagttttggt ttgcattgat caaagttgta gcgatcattg cctttatcct cgtaggagct
    20221 tatatgatcc tcacccacta tacgacagat attggcacag ccagtatcac gaatctatgg
    20281 tctcatggcg gattctttcc aactggtgca aaaggattta tcttagcttt ccaaatggta
    20341 acctttgctt ttgctgggat agaattagtt ggattagtcg ctggagaaac agaaaatcct
    20401 gaaaaagtgt tgccggaagc tatcaataat attcctatca ggatcatctt attttactta
    20461 ggatctcttt ttgtcattat ggcaatctat ccatggaaca gcttgaatcc agataacagt
    20521 ccatttgtag aagtattttc ggaaattggg atcacgattg ctgcttcctt gatcaattta
    20581 gtcgtattaa gtgcagcagc ttctgcctgc aacagtgcaa tatatagtac aggacgtatg
    20641 cttcgttctt tagcgcaaga aggcagtgcc cctaaaaaat tcaaaaagtt aacaacacat
    20701 catgtgccag ggaatgcttt gacattttct actatcgtca ttttcatttc tgttatcttg
    20761 aattacgtga tgcctagtga agtattcaca ctagtaagca gtattgccac tacttgtttc
    20821 ttgtttatct ggagtatgct tgtctacacg cacatgaaat atcgtaaaag tatcctcggc
    20881 aaaaaagctc attcatttaa aatgccgttg tacccattta gtaattatct ggtatttctt
    20941 tacatggcct ttgtgtgtgt cgtcttgttc ctaggaaaag atacgagaat cgctttatta
    21001 ctaacacctg tgtggttctt gcttctatta ctaatctatc atatgaaata tgaacgtaag
    21061 aagtaagaaa gtagttgtga cagaagtggt cagcttcgag aaataagaag gaatttacga
    21121 aaattgcttc ttaaattttt gtgaaatttc ggcttatttc ttggagataa aagcttctgt
    21181 cccagcctct tttctatttc cccggaaaca accgctgata ataggcatct gcttttttat
    21241 aggaaaaaat ccaaatacca gatacaaaaa aacgtccgaa taacccgatc aacggtacaa
    21301 aaaagacacc ggcaatcaag cataagttaa tcatcatgga gataagactt cgacggtcct
    21361 ttgacaaaac tctcacgaca tgggaatctg gtttatttgc tttcatgatt tgcctcatca
    21421 agataccaaa agaaaaactc cacatcgtgt atactaagac ataagccatc tcaggccaaa
    21481 aagcttcagg gttacgcccg acccaactag taacagaagg acataaagtc agccaaaaga
    21541 gcaaaaagat attcgaccag accactcggt aattaattcg ttctgccatc tgaaatatac
    21601 gatgatggtt cacccagatc accgcaacaa atataaagct gatggtataa gctaataatg
    21661 tatttcggat gacccataaa gaatgaaagc tagatgtatt agggatcttg atctctaata
    21721 caagaactct caaaacaatg gctaccactc catcagtaaa tgcttctaat ctcgactttg
    21781 tcacgatgat tcctcctcag atgattcagc gcttttattt tatcacttac gttattaaat
    21841 aaccatcgtt ttatataggg aattttctga aaattgttgc atatttttcg aaattttgtt
    21901 acttttaaaa aaaccgtaaa ggagttatgt atgacaaaaa aattatcatt taaagactat
    21961 ctttttatag gctccatgct tttcggtcta ttttttggtg ctggaaatct tatctttccc
    22021 gttcatttgg gtcaagaagc aggcgctgct accttttggg cgaatctagg tttcttggtt
    22081 accggtatcg gtcttccctt cttaggggtg attgccattg gtgtatcgaa aagctcaggt
    22141 gtatatgaat tagcaaaacg gataaatcaa acatatgcga tggtttttac catcttgctt
    22201 tacttagtta tcggaccgtt ttttgcttta cctcgccttg caaccacatc tttcgagatt
    22261 ggtttagcac catttatcga tcctagcaga cagacgctat atctagcaat tttcagtatt
    22321 gtattttttg tgctggcttg gtggttttca agaaaaccga caaagatatt agattatgtt
    22381 ggaaagttcc tgaatcctgc atttttggtt ttattaggtg ctttgcttct ccttgctttt
    22441 ttgaatccac taggttctgt aaatcaagcg cctattcagc caaattatca agagcatgct
    22501 tttttcacag ggtttactca aggatataac acattagatg cgcttgctgc tttagcattt
    22561 gggatcatca ttgtcacaac aatccaaaac atgggtgtaa caaaacctgc tgaaatcgct
    22621 aaagacacga tcaaatctgg tgcgatcagc attatattga tgggaatcat ttatactttg
    22681 ctcgcttatt taggtgctat gagcttaggt agctttgctc taagtgaaaa tggcgggatc
    22741 acgctggctc agattgctga tcattattta ggtacttatg gcagtatctt attagcattt
    22801 atcgttatat tagcttgctt gaagaccggt attggtttga ttacagcttt tgcagaaaca
    22861 ttcacagatt tgtttccgca aagaaattac gcattctttg ttgcacttgc tagtatcctt
    22921 ccttgtttag cggcaaacgt aggcttgacg aatatcattc aattttcgtt gcctgttttg
    22981 atgttcatct atccattagc aatgacactg attttactag tgttagttgg tcctttgttt
    23041 aagcagcgta cggctgttta tcgaatgact acttatttta cattgattgc ttcgattttt
    23101 gatgggttga acgcttgccc agaaactatc aaacaaacac ctattgttca aaatctttta
    23161 tatgttgcgg aaagctactt gccgtttttc aaattgggga tggggtggat cgtaccagca
    23221 gttatcggtt ttgttatcgg actgatttgg agtttcgcta agaaagaaga agtagcagat
    23281 tgataaaaga ggttgtgaaa gagacgttca gccttgagca ataaaaagta attttgaaaa
    23341 atggcttctc acatttttgc ataaatttaa cttaataccg agaaactgac ttttgaacac
    23401 cgtttattga gaggtcgtga aaaagccgct tagtttcaag tattaagaaa tattttgtga
    23461 aaatggcttc tcacattttt gcataaattt gacttaatac cgagaaactg gcttttgaac
    23521 accgtttatt gagcgtctat aaactgacct gagtattaga gaaacaaaac ggcaaagtag
    23581 ctccatatac tttgccgttt tgttttgaac taataccgaa gagaagctga tcaaacaaca
    23641 tttcttttat aatatagatt ttagacatat atatgatata aaaatagttt tcgcaaaa
//
LOCUS       DACDPS010000037        23644 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.037, whole genome shotgun sequence.
ACCESSION   DACDPS010000037 DACDPS010000000
VERSION     DACDPS010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.037"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            46..1386
                     /locus_tag="IWJ07_001558"
     CDS             46..1386
                     /locus_tag="IWJ07_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9333141.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1604..2047
                     /locus_tag="IWJ07_001559"
     CDS             1604..2047
                     /locus_tag="IWJ07_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAP9333142.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(2551..3969)
                     /gene="nhaC"
                     /locus_tag="IWJ07_001560"
     CDS             complement(2551..3969)
                     /gene="nhaC"
                     /locus_tag="IWJ07_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP9333143.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(4372..5595)
                     /locus_tag="IWJ07_001561"
     CDS             complement(4372..5595)
                     /locus_tag="IWJ07_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAP9333144.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(5626..6465)
                     /locus_tag="IWJ07_001562"
     CDS             complement(5626..6465)
                     /locus_tag="IWJ07_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP9333145.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(6482..7162)
                     /locus_tag="IWJ07_001563"
     CDS             complement(6482..7162)
                     /locus_tag="IWJ07_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9333146.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(7164..8237)
                     /locus_tag="IWJ07_001564"
     CDS             complement(7164..8237)
                     /locus_tag="IWJ07_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9333147.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            complement(8884..9555)
                     /locus_tag="IWJ07_001565"
     CDS             complement(8884..9555)
                     /locus_tag="IWJ07_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9333148.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(9642..12320)
                     /gene="mgtA"
                     /locus_tag="IWJ07_001566"
     CDS             complement(9642..12320)
                     /gene="mgtA"
                     /locus_tag="IWJ07_001566"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAP9333149.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTRFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            12979..13416
                     /gene="ndk"
                     /locus_tag="IWJ07_001567"
     CDS             12979..13416
                     /gene="ndk"
                     /locus_tag="IWJ07_001567"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAP9333150.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(13438..14304)
                     /locus_tag="IWJ07_001568"
     CDS             complement(13438..14304)
                     /locus_tag="IWJ07_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9333151.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(14464..16557)
                     /locus_tag="IWJ07_001569"
     CDS             complement(14464..16557)
                     /locus_tag="IWJ07_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP9333152.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(17306..17929)
                     /locus_tag="IWJ07_001570"
     CDS             complement(17306..17929)
                     /locus_tag="IWJ07_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333153.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(18088..19485)
                     /locus_tag="IWJ07_001571"
     CDS             complement(18088..19485)
                     /locus_tag="IWJ07_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9333154.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(19517..20488)
                     /locus_tag="IWJ07_001572"
     CDS             complement(19517..20488)
                     /locus_tag="IWJ07_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9333155.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            20862..21287
                     /locus_tag="IWJ07_001573"
     CDS             20862..21287
                     /locus_tag="IWJ07_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333156.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            21349..21546
                     /locus_tag="IWJ07_001574"
     CDS             21349..21546
                     /locus_tag="IWJ07_001574"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333157.1"
                     /translation="MIVKETKWMIDSPDYCTCNNNKNIHTEFNDQGSLSICDNCHKLV
                     KDSFKTFESYPVQNYVKSRRR"
     gene            complement(21775..22476)
                     /locus_tag="IWJ07_001575"
     CDS             complement(21775..22476)
                     /locus_tag="IWJ07_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP9333158.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(22492..22932)
                     /locus_tag="IWJ07_001576"
     CDS             complement(22492..22932)
                     /locus_tag="IWJ07_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP9333159.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(22932..23507)
                     /locus_tag="IWJ07_001577"
     CDS             complement(22932..23507)
                     /locus_tag="IWJ07_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAP9333160.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT         6805 a         4902 c         4067 g         7870 t
ORIGIN      
        1 cttaatataa attttgattg tgaatttaaa agaaagaggt catttatgga agataaaaaa
       61 ttgaaaaaac aacttagctc cagacatatc accatgttag ctttaggtgg agcaatcggt
      121 gcaggtctat ttaaaggaag tggcgaagcg attgggatcg ccggcccttc tgtattgatt
      181 gcttttttga ttggtgggct gattttatat atcgtgatga aaggtctggg aaaaatcgtt
      241 ttaagcggtg gcgacacgca tcacggatta tctggtttga tacgccctta tctaggtgct
      301 cattctgctg attttactga ttgggtctac tggtctatgt ggattatcaa tatcatcgct
      361 gaagctgttg cagctgcttc ctttttgcag ttgtggttcc cgcacattcc agcctgggta
      421 tttgttttta tgttggctgt tttaactacg atcatcaatc tttattcggt acggttattc
      481 gcagaaacag aatattggct ggcttttgct aaaatcagtg tcattatctt actgatcatt
      541 tttgctactt atcttgtcgg tcagcaaatg ttgggcacag gcgttttccc tacccttcag
      601 caattgactg accatggtgg gtttactcct catggtatga agggaatcat cagttctttg
      661 cttgtagtta tctattctta cggcggttct gaactgattg ctatcacggt aagcgaagca
      721 gatgatccta aacgtgcgat tcctaaagca atcaaaggtg tcatgggaag aatcatttcc
      781 ttttacatca ttcctttgtt tttgcttttg attctttttc cttggaatac tttagcaggc
      841 acgaatgtca gtccttttgt gatggtattt gaaaaaatga atatcccatt tgcagcggat
      901 atcgtcaatt ttgtgattgt ccttgctttg ttctcatcta tcaattcggg tgtgtatgca
      961 tcttcaagaa ttttgttctt ccgtttgaag gatcgaaaag gttcctctag aaaactagct
     1021 gttttaaaca agcatcaagt accgcaacgt gctgttttat tttgtaccag tgttctttat
     1081 cttggtgtag ctctttctta ttttgtcggt gacaaattat ttggctatct ggcaggttcc
     1141 ctttcgtata cagtattatt gatctggatc attatcagtg cggcatcctt tgtactggca
     1201 ctaagaaaag gaacattatg ggataaagga atcagctttt ttgcattagc tgtcttagga
     1261 ttgattttcc ttggtatcct gttcacgaac tccattgggg taactgtatt gactggcctg
     1321 ctttacctgt tcatttattt cagttatcaa aaaaagaatg atgcgttcgt attgacgaat
     1381 gaatgaaatc gatagaaaat tttattttat ttatgtggaa tagatggagc tattgaagaa
     1441 aggagttgtg acaaaagtct tgttgcaact cctttttccg aataaacagt ctcatgacct
     1501 cttggtaaac ggtgttcaaa aaccctctgt gcaaaataat tgattgttct ccatgctcat
     1561 gacatgatag aagaaaggaa tgaaaaaaag gagtttgttt taaatgaaaa agtattggaa
     1621 gtttttgttg atgatcggtg tttcaacggt agttatgttt ggtatgatgt acttgaatgt
     1681 ctatgcgctc gatcatatct tcttcagtcg tacacgagta ttcatggcgt tgatgatggg
     1741 tgcagtcatg gcagtcatca tgctgttgtt catgtggaaa atgtatgaaa acaaaaagat
     1801 aaatgttatg atcttagccg ctagtcttct tgtttttgcc ggttccttgt ttatggtacg
     1861 aagccaaact actgtcaacg acacagcttg gatgaaggcc atgattcctc atcattctat
     1921 cgctatcctg gcaagtaaac gagctgattt aaaagatcct gaagtaaaaa aattagcgga
     1981 agaaatcatt caggcacaag aagaagagat cgacaaaatg aaaaaaatga tcaaagaaat
     2041 ggaatgacca ttatccgtcc aaataaaaaa agaggatgtt ccagaagtta tcagcttcaa
     2101 gaaataagaa gaagctactt ctggaacgcc gtgaataagg ggttgtgaaa aagctgccct
     2161 gcatcaagta ataagaacag ccaaacaaaa atagcttctc ctatttttcg tttggttagg
     2221 cttattaccg cagatgcaag cttttgaaca ccgtttatta agaggttgta aaatcagcgt
     2281 tctgacctga gtattagaga gacaaatgga aaaatagttc ctcatatttt gccatttttt
     2341 gagctaatac cgagggtcag ctgattgaac accgtttatc gagaggttgt gaaaaagtcg
     2401 cttagtttcg cgtattaaga aaaattttgt gaaatggctt tccacatttt tgtataaatt
     2461 tggcttaata ccgagaaact ggcttttgaa taccgtttat tcgtaaaagg gattgtgaca
     2521 aaagtcttgt cacaatccct ttttatgttt ttattgttct aattgcgctg tcttgcttac
     2581 tgcttcttgc ggtttcacaa atttttttga gattccccaa cctgtgaagg cactgaccat
     2641 cgaaaacaca ggagaaagca aactgaaaaa tacgaatggc aagtaagtga gtgtaggaat
     2701 accaaatgta cttgctacga aacttccggc aattccccaa ggcactaagt agttgattac
     2761 tgttccacca tcttctagcg ttctacctaa tacagttgga tcaaatcctg ctttttcata
     2821 agtttcttta aatgcattac caggcagaat aacagatagg aattgttcac caataaaaat
     2881 atttactccg atacttgtca tcaatgttgt aaaaatcaaa tttggtgtgt tcgtcatctt
     2941 tcttgatact cgttccatca cggttgagat caagccaaat tcgatcaaca atccacctaa
     3001 tgacaaagtt agaataatca atgacacagt aggcatcata ctttcgattc ctccgcggct
     3061 aagcagtgaa tcgatttgtt ggtttccagt atttgaaaca aaccctgatt cgatgacccc
     3121 agccaacttc gttaaggaag tttgtgggtt ttgaataaaa atcattccta ctgctacagc
     3181 gatattcaac aaaatcgtta aaattgccgg cacctttttc catgcacaca gtaacattaa
     3241 aaataaagga atcagtgacc agattgaaat agtaaagttt gcttctagaa catcagtcac
     3301 ttcacgaata gatgaaagac ttgcggcttt acttgtgtgt cctaaaatca tgaataaaaa
     3361 gaaagaaaca acaaatgccg ggacagttga ccacatcata tgcttgatgt gtttgaacaa
     3421 atcggcatca acaatagctg ctgctagatt tgttgattcg gataatggag acatcttatc
     3481 tccaaaaacg gcacctgaaa caatagctcc aacaaccagc gctggattta agcctagtgt
     3541 tgtcccaata ccgaaaaatg caatcccaat cgttgacata acagtaaagg cactccctac
     3601 agcgctacct acgattgcac acacaacaaa tacagaaggg acaaaccact ggacactgat
     3661 caatttgaac ccaatgacca ttagagttgg aatgatccct gcttgtatcc atacagcaat
     3721 caatgaaccg actaaaataa aaataaagat tgggatgatt cctggtttga tgccattgat
     3781 gattccttcg tgcagccaat ctgtcgggta tctttttatc actgcataca acatgaccat
     3841 tccgatcgct gctaagatcg tgatattagg tgagaccttt aatccgacaa cgccaatcgc
     3901 aatgcctaaa acaataacca ttaatacaag tgttgcttct aaaaaacttg gtgttccttt
     3961 ttttcccatg ataatctctc cttttttagt tgttcttatg ttctaaaaaa gttaagatat
     4021 cggattactc cgccgcaatt catccgcgat tcctccttta taaaaataaa aagacctgtt
     4081 ttaaactaag gacgaatgac tccgcggtac caccttaatt aaaaacagat ctgtttttca
     4141 ctttcaaact tttattcaaa tgtatggact ttccatgtaa ttcgtcaatt aggcttgatc
     4201 ggttcacagc gaccaccgac tttctgaaca aaacacctaa ccgctactgc gtgaaaagtt
     4261 gacttgtacg aagtttacct tccataagat catttgtcaa tgaaaaagaa aatatttttt
     4321 catttttctg aaaattattt ttataacttt cacacacaag tttatttttt actagaaagt
     4381 agcgccctct tgtttttttc ttgctactat ttcttgatgt tcttgcttga ttctttctaa
     4441 tagctttggc tctttcaaca gatcaagagc tgtattggcg agtagttctg ctgcaatcgc
     4501 aatagaagca aggcctttgt cacttttagc agcagctttg aattcttctg tgtgtccggc
     4561 aatatatgtc tcagagattg ctaccgtagg ctggattgtt ggtatgactt ggctgacatt
     4621 tcctacatca gaagaaccga gacttgttct ttgttccgtt tctatttctt catctggaac
     4681 acctgcttct tttgcatgct ggaagaataa gtcatcaaaa gaaggagtaa cgatgacatc
     4741 atcaactgca ttttggaaca atccaaactc aaaagtcgtg cccgtactta tcgcagcacc
     4801 tcgtacgata ttttccactt tttcatatac tttatctaat gtttgccgat ttcctgcacg
     4861 taaatagaaa cgtccagcag cgtaatctgg aatgacgttg gcagctactc ctccgtctgt
     4921 aatgatccca tggatattga catcttttgg caattgcaag cgtagagcgt tgattccatt
     4981 aaacacttgg atcaatgctt ccaaagcatt tactcctttt tcaggtgctg ctgctgcatg
     5041 ggaagcgaca ccgtaaaatt tgatgtcgac tggatcattc gctaaagatt ctgtcgtttt
     5101 cccataacga aaagctggat ggacgcataa cgctacatcg acatcctcaa aaaaaccttc
     5161 tcgaacaaag ctgcctttag ctgatccgtt ctcgccccct tcttcccctg gggtgccata
     5221 tacacggatt tctccaccag tttcatcaat gaattgcttc actatgctgg cggctaatac
     5281 ggagtaagta ccaaacaaat tatgcccgca tgcatgacca atacctggta aagcatcgaa
     5341 ttctgccaaa aaggcaattg tgggaccggg tttattggaa atataacgtg catcaaaggc
     5401 tgttcggtgt ccagctactt ctttttctac ccaaaacctt tctttttcta attgtttaat
     5461 caacgctgct gaggaataaa actcatagtt acttacttca ggatgttcgt ggatatctag
     5521 agcaagtgct tgatattctg gtaaacgata ttggattaga tccttcaatt ggtttgttga
     5581 gattgttgtt gtcataaaaa attcctccta tgctagtcat ttttcttatt ttctcccgaa
     5641 tgtttcccat gcaggcacgc ttgatccttt tgatgttttc tcgattactt tgactgtgtc
     5701 ttctgtttga taagcgtcta ctattttttg atacgtttta ttgttttcat cttcttttct
     5761 tgcaacgatg atattcacat aaggacgtgc atggtcatcc actggttcta agaagatggc
     5821 atcagaagca ggattaaatc cagcatctac agctacaccg ctattgatca ctgaaatgtc
     5881 tacatctgac aaagcacgtg ctgtttggga agcatctaat tcagaaatgt ctagattttt
     5941 tctattctcg gtaatatcag aaacagtggg cgcttgtcct tttgccttat ccactttgat
     6001 caatccagca gtttgcaata acagaagagc tctgccgcca tttgtaacat cattagggat
     6061 tgctactttt gctccgtctt tgatatcagc tacatccttc aaagtgttag aataaattcc
     6121 cagcggtgca ttgacagtat ttccgatcga tactaaatca gttccgtgtt ctttattgta
     6181 attgtctaaa aagaattgat gttggaaaga attcaaatca atcgatccat ctgctaaagc
     6241 cacatttggt tgattgtaat ctgtaaattt cacgtattcc aaattaatgt tctccttttt
     6301 caaacggtct ttcacgttgt cccatacatc tgtatcttct ccaataatcc ctagttttac
     6361 gatagtttct tttttgtcag ttgtatctgc ttttccttgg ctacaccctg ccaaccaaat
     6421 aattgctaca aatgtaatga ctacaaaacg aataattttt ttcatttttt ctcctccact
     6481 ttcaatgtgt aattttttta atcagttgat tactgataaa ttgacttaaa aaaactaaga
     6541 ttagaatcaa taatgtagcc acaatcgtta catctgtttg gaatcgatta taccctctcg
     6601 atatagcaag attccctaat ccgcctgcac ctactgctcc agccatggta gtcagtccga
     6661 tcacgttgat gatagtcaac gccgagaccc gaatgatacc aggcaaacct tctaacaaat
     6721 acacacgtaa aatgataccg attggactgg ttcccatcgc ttctgccgct tcgatcaccc
     6781 caggatcaac ttctagcagt gcgttctcga tttgacgagc aaaaaacgga acaactccaa
     6841 aaatcaatgg cactaaagct gctttcgcac caatcgtcgt tccaactaag aaacgagtag
     6901 tcaatgctaa aagagaaagc aagataatga aagggatgga acgaatgata ttgatcagct
     6961 tatcaagtat ttcaaatagc agacggttag ctagtaatcc attagggcgt gtcactacta
     7021 acgtcacacc taagatgatt ccaatcatcc cgccaatgaa tgctgtccaa aaagacatat
     7081 atagtgtttg cttcgtcgct tcgacaaatt catccggaat caaataaaca ttgggcaaat
     7141 atgttttaaa gaaatcagtc attttatctt cctccttcga tcactttgat acgactatct
     7201 aaatcgtttt tttgtaaaat atttattttt acaccagatt tgatcaaata atccaatgct
     7261 tttttccgct gtttggtttc tccagctaaa gtaacgatca aactccctaa cggcgtttct
     7321 tgtaataact ctacattgcc atataaaata ttcgctgtta cttgatattt gctgtacaga
     7381 tgagcgatta gaggttcgtt cgtctgattt ccaatataac ttaattccac cagccattta
     7441 ttggcgatct gatcggcaaa agcagaatgg gcaatgatcg tttctaatgc ttggtcaaga
     7501 tgagtagctg ttcgaataaa atctttggtc aattcttctt caggattgct gaagatctgg
     7561 atgctgctac ctttttcaac gattcttcca tcttccataa ccgctacctt gttgcagatt
     7621 tctttgacaa cttgcatctc atgagtgatc aggacgattg ttaaacctaa ctggcgattc
     7681 aattttttta acaatgcaag gatctgaagg gtcgttttcg gatcaagtgc actggttgct
     7741 tcatcacata ataatacctt aggttcattc gccaaagctc gtgcgattgc tactcgttgt
     7801 ttttgtcccc ccgacagttg tttaggataa gcagatgctt tttcttccaa accgacaagt
     7861 tccagtagtt cattcacttt ttgacgacgt tcctgtttcg attttccaga atactttaaa
     7921 gaaaaatcaa cgttgtcaaa aatagttcgg ctttccatta gattgaaatg ctgaaagatc
     7981 ataccgatgg cctttctttc ttttcgcaat tctttgggag agagctgact cagattcgtc
     8041 tgattgacaa tgacttctcc tgctgttggt ttctgcagca aattcatcac acgaactaac
     8101 gtacttttcc ctgcacctga gtaaccgacg attccataaa catcgccttt ttcaatcgtt
     8161 aaatctacag aatctactgc ctgtatctct ttttcttttt gtttgaaaca tacagaaaca
     8221 tctcgcaatt caatcattaa aattcctcct tatctaaaaa aagcgtcctc aagaaaatga
     8281 aaatgtcatt ttcttgagga cgcttttttg cgtgttacca cctcatgttc aagctgtgtt
     8341 cacacacaac tcctcttcaa gtacgatcat actctagcaa gatatcggtc gcagccgtca
     8401 tcgccttaca tctatgctcg ggatgaattg ctcgatggcc attttccaat agtcgttcct
     8461 tgcttcttct cagctaccga agctctctgt caaaggttct tctatcgtac ttaccatttc
     8521 aatgcaaata atattcaaaa aaaaagtcct tttactaaca taaactatgt cagtaaaagg
     8581 acgacgtatc gcggtaccac cttttttcgg aagtttcctt ccctcaagcc caacatctca
     8641 aaaagagaga ttggcgttcg ttttaacggt cgaacagttc cgaatttgcc tacttatcga
     8701 caaattattc tccaaggcca ttttcagctt ttcttacttg actcttctca ccagccagag
     8761 cctctctgca aagtttccaa acttactttc cttttcaacg aaatcgatat tttgttgttg
     8821 ggattaatct acatgaggaa aactgaaaag tcaagtacga aattcaaatt attttttcta
     8881 tatttatttt tctgtttttt tatttaacat ttcaataaca gttcgaagat cttttccttc
     8941 cgcaagaagt ttcaattttt ccttttcttc gacggtataa tttcggacaa aactggaatc
     9001 aggtatcaaa acaacgccta atcctgctcg tgaagcagct gttgctccta ctaaagaatc
     9061 ttccactgca aaggctgaac tagcttctaa tcctgttcgt tccaaagctg tcagataagg
     9121 aatcggtgaa ggcttgttct ccgacacttc gtctccgaat gtaaacgtgt ccaagtaatc
     9181 agctaattta aagtgatcca agatatctgt tgctcttttc gtaacagtgg tcgtagcaat
     9241 gcctagtttc atatgacgtt cttttaagtc tgccaatatt tcttttgcat atggttttaa
     9301 gtggatggct ccgcttgtca gctgttgata aaaatattct tcacgcttcg ctcggacttc
     9361 ctgtactttt tcagggctac ctgctgcttt cactaaaata tcaaaacttt gtttccagtt
     9421 ttttccgccc caagcgtcaa cgatttcttt tgggatatgg atatcaaatt gctgaaatcc
     9481 ccaaagccag ccatcacgat acgttttttc tgtgtcaact aacaaaccat ctaaatcaaa
     9541 tattgcacct ttcatctata cattaatcct ttccaaaaca atacacatag tttagcacga
     9601 ctggtccaaa aagaacagaa gcaaagaacc agccttctcg tttataagag ttcttggaat
     9661 cgacggatat agattttctt cacagctgtc acaagcgcca gataagcgat gactgtagca
     9721 aataaccata accagtaatt gccaggaagt ggcatcaagc ctaattcagc accaaacctt
     9781 gtaaaaggaa ggacagaacc gacaataatt cctaatgtag tgataacaaa catggcaaat
     9841 gttgcgttgc tttggataaa tggtactttc ggtgtacgca gtgcatggag cactaaggtt
     9901 tgtgtccata gtgattcgac aaaccatcct gcatggaata atgcgatgaa aatcactcgc
     9961 tgtgaactat ccaatgtatg gaaacttccg cccactacag caggacaaat aacaaaatac
    10021 atcaacaagt aagtagtaat atcaaatatc gaacttgttg gaccaaagta aaccatgaat
    10081 tccccaatac tagatgcatt ccattttttt ggctcgtcca aatattcttt atccatccga
    10141 tcccatggca aggaaatgca ggaaacatca tagatcagat tcaaaaacaa aagttgtaaa
    10201 ggcagcattg gtaaaaatgg caggaacgta cttgcaatca gcacggaaaa catattgcca
    10261 aaattagaac ttgctgtagc cttgatatat ttcatgatat tcccaaaagt ttctcgtcca
    10321 gaaagaaggc ctctttccaa aatcgtcaaa tctttttcta aaaggatcac gtctgcagat
    10381 tctttcgcaa tatccacagc tgtatctaca gaaataccga tatcagccac tttcattgca
    10441 ggagcgtcat tgataccatc tcctaaaaat ccgacagtat gaccattttt acgtaaagct
    10501 gtcgtcaaac gtgctttttg ctgaggattc aattttacga atacttcatg gtcttcagct
    10561 acttgtgcta attcatgatc actcatttcc gagattttct caccggtaac caattcattg
    10621 atctctagac ctacttgacg gcacactgaa cgtgtaacta attcgttgtc cccagtcaga
    10681 acttttacag ctacaccatg ttctttcaga gctttcaacg cttgtttcgt tgtttctttt
    10741 ggtggatcaa ggaaagcaag ataaccaatc aaaaccatgt ctgactcatc tttgactgaa
    10801 aattctccaa cgacgcttgg attggtcttt tgtgcgacac caatcactcg taagccatct
    10861 tcattaagtg actgtacttt tgcaagtacg gcttcttttc gttcttttgt taatggtgta
    10921 acagtaccgc caagatcaat aaatttagaa atgttcagca tttcttcaat cgcgcctttt
    10981 gtgatcattt gtgttttacc agctggatca gcaaccacaa cactcatacg acgacgttca
    11041 aaatcaaaag gaatttcatc cactttttga taattgatct tatctgtatc taaagtttct
    11101 gttgcttcat caatgatagc cacatctaac aaatttttca gccctgtttg atagtagcta
    11161 tttagatacg catggcgcaa aacacgatca tcttcatttc cttcacaatc catatgatat
    11221 tcaagaatga ttttatcttg tgtcaatgtc cccgttttat ccgtacataa cacatcgatt
    11281 gcaccaaagt tctggatggc attcaaattt ttgatcactg ttcctttttt ggccatcaca
    11341 cttgctcctt tgaccaaatt cgtcgtaacg atcattggaa gcatttcagg tgttagacca
    11401 actgctacag atagtccaaa aagaaaagct tctagccaat ccccttttgt aagcccattg
    11461 atgacgataa ctgctggagc catcaatgac ataaatttga tcagtagcat ggatgtttta
    11521 tggatgccta attcaaagct tgattgaatt ggtttttcag atagcgtttc tgctacatga
    11581 ccaaatagtg tttcatttcc aacagaaaca atgattcctt ccgcacttcc actgatgaca
    11641 ttacttccca taaagacaag attttcgtaa cttgtttcgc tttgctgatc gtcttcacat
    11701 actttcgcat gcttttctac tggatagctt tcccctgtca atgctgcttg ggaaatgaat
    11761 aaatcctttg cggacaataa acgcatatcc gctggtatca tatcccctgc tgaaagccga
    11821 acaagatctc cacagacgat ttcttccata ggaatctctg tgtactttcc attacgtttt
    11881 acagctgccg ttactttcac aagtgatttc agtttttcag ccgcttgatt ggaacgtacg
    11941 gactgaatca acgtcattgt cccgctgacg ataaccatag tgaagataat ggctacaccg
    12001 aaaagatctt tatctccagg agctgctaat acatattccg tgatgaatga aataattcct
    12061 aatgcgatca acactaatgt aaatggcgtg atgtatgctt tcaatatttc tacaatgaat
    12121 ggtgtttttt tgccatatga aatcgcattt tcaccgtaat tttctcttga acgttcgatt
    12181 tgttcttctg tcaatccatt tgctgatgca tggaatagtt caaataattc ttgtttcgtt
    12241 tttccagcaa aagctgcata gattttttgt gtatctttct ttttttccat ctcaatctct
    12301 cctttgtgtc caaaagacag ctgttctcgc tgcttttggt ctttagctat tcatcggatc
    12361 gatagagaaa aaactgaaag gggctgagaa cagatagata atagccctgg cctttcctct
    12421 agatccttgt ttttcgaatg actgtcatcc atttgttctc atctccttta ctaagtaatt
    12481 ttaattcatg acttccaatc atttttcgaa tagttatttt tccaattaga ccacctcctt
    12541 tttatttttc accgatgggg atttgcggca acaaaaaaac ctctcgccaa atactcagcg
    12601 aaaggtttga aaaagcatat taaaaaaagc ttctcagatc tcatcgttga gttttgacac
    12661 tgtacaacgt aaaaagcaga tacctgcttt tagctacctt atgaaaagcc ttatctcgac
    12721 aattcctgtt ctacccattg gcgtctctcg acgtttttgg gcagtagcct atgtttgtat
    12781 aggagcctca cctaacaaat cttattcggt tttccttcgt taatataaca actaaaaaag
    12841 aggatgtcaa atcaattttc catgtttttc atactttttt taaaatacac acgttatatt
    12901 cctgtctcac gcggttttat ttgtctcatt ttccacctcc cgctataata gaaatatagt
    12961 aggaaacgag gtgctgttat ggaaagaaca cttgttatta tcaagccaga cggcgtgaga
    13021 cgccatttag tcggaagcat catccaacgt tttgaagcaa agggactggc cattgctgaa
    13081 atgaagtttg aaactatgac tcctgaatta gcaaaagaac attatcagca cttgactgag
    13141 cgcagttttt ttgatgaact gatcgattac atgacttccg gtcctgtcgt ttatcttgtg
    13201 ctggtcggcg aggaagtgat cgatatcgtc cgtaagatgg ttggagcaac aaaagctgcc
    13261 gatgctgttc caggtacgat ccgaggagac tacgcgctgc cggggactga aaacatcatt
    13321 cacgcttctg attcccgtga tgctgcggta aaagagatcg cccgcttttt cccgccattc
    13381 gatataaaca aaccgtctga aaaagcggcg aagtgataca aattctaaac tgctggtcta
    13441 gttgctgggt tcagcaatgg ccagcagttt tttctctaag gacctccgtt gattctttcc
    13501 taacatatat tcgagcactg tgaagaattc ttggtagtct ttcgtctttt cttcccctaa
    13561 agcagcagaa atcaatcttt gataataatc tgccatcaat gtttcataga tcactgtact
    13621 tctttcttta tgtgctaagc tccgtttcat atcttcattc acactacgtg cctttttcaa
    13681 ccgaccattt tctatataaa acgaagtcag gttcattaaa aaagtaaaga gatactgctc
    13741 atgattgttc aactgtgcta atcgtcgcat tctttttttg attttcaaat aattgttctc
    13801 aatgaaccga tcactgaata tccaaggaaa atcagctaga atagatagtt caaattgacc
    13861 atagtctggt atctgcatca agtattcttc aatgattcgg cattcataac gtaaaaattc
    13921 aaattctgtc tcagatactt ctgttttttc gaccatgtga agattcaaaa ccaaataata
    13981 cagatcttct gttttcttaa aatgcctacg gttattgctg atctctttat aaggatcaaa
    14041 gctgtcttct tgtacccgat ctagcattcg tctttttttc tttaacttta ttggttcatc
    14101 cagctggttt cgcatccatt catattctgc aagtgtcatg ttcattcgct ctaaaaatga
    14161 tctcaatgtt acaaatgaca tttttttcac tgaatttgtc tcaatcccga ctagagaagt
    14221 cttaaaacag attccttccg ctaattggga ctgtgtcaac ttcttgttga ctcgaagttt
    14281 cttgatcaat tcaccatcat acataatagt tctccttttt aatttaaatt tttccacatt
    14341 caataaatag atacgttaaa aaggaaaaat acacgaaaaa aataaaaaaa tgtttttttc
    14401 ataaaaaata ccaaccttca agcattagaa tgttgtctaa ctactgaagg ttggtttgat
    14461 tgtttattga ttattttttt gctgttcaac gtctgttctt gttactggat ataagttggt
    14521 tgttgtttta ttaccatttt ttgaaaagac acttgtcgaa tgttcgcctt tttgcttttc
    14581 gattgcttca ttacgttccg cttcattttt atagtcatat tttttcggat caatcgtctc
    14641 aaatccgttt ggcacataaa accgcaagag attttcctga ttcagcttat ctgaaagact
    14701 taacgcagtc ttgacttttt cctgatcttt ggagatttcc tcatcgattc ctgctgcttt
    14761 cgtatcgatg atttctcctg tctgattatt gtaagctttt cctcctagga cggtgtattt
    14821 gggtgtcaca aagttcccat ttcgaaaagc aactacttga tcatgctgag aagataataa
    14881 gtctgtacca aaatgaagat aatctttatc atcgattccc aatagatgta gtaatgtcgg
    14941 caaaacatcg atttctccac cgtattcatg attgactgtc cctttagtat aaccaggcat
    15001 atggatcatc aatggcactc gttgcatctg tgcgttgtca aattcatccc atgtatctgg
    15061 atctttacca agagcgcttg ctaaatcttt attatctgta ttcgagatcc caaaatgatc
    15121 cccgtaaatc acaaagattg aattttggta aataccagat tttttcaaat acgtaaaaaa
    15181 ttgttccaat gattgatcca gataatgtgc cgttcgaaca taattatcta ctgtgctatt
    15241 gccagtattc aaagatggaa agtcgaaatt cttgtcatcc gtataataag gcgtgtggtt
    15301 ggttacggaa agaaatttcg tgtaaaacgg ctgttgtaga tgctcaagat attgggcaga
    15361 ttctctgaat aaatctttat ctaatatccc ataacctaac gtttcgtcgg attcatcaaa
    15421 ataagatcga tcaaagaaat tgtcataacc taagttttta tatacatgat ctctattcca
    15481 aaaactagct acattcccat ggaaaacagc gcttgtatag ccttgctttt gtccaaggat
    15541 cgctggtgcg gcttgaaaga cattgtctga cccaagttct gtgaataatg agccttgagg
    15601 taaaccaaat gtgccagttt ccaacatatt ttcagcatca cttgtctttc cctgtcctac
    15661 ttcatgaaag aaattgtcaa agctgatcgt tgcttgattc tggaaaatac tatttaaaaa
    15721 cggtgtgact tcttgtccgt ctaccttcat attaatcaaa aactgctgga aactttctaa
    15781 atggagaacg atcacatttt tccctttagc gatcccaaat gtttcgggat ttggctctgc
    15841 ataatgtttc tttgtataat ctaaaacttc atcaatccca ttactgcttg catgcgcgcg
    15901 aacactgcta gtcattcctg ttttgattcc gtcataaata gtatacgcat cgattcctag
    15961 atatttgaca atatatgaac ggtcaaatgt tcgctgcagc aattgtggac ggttggcttc
    16021 gctcagcgct agatttcctg aaaaaataag ccctgaaaga caggtaacag cgatagccat
    16081 cggtttgccg acagggttgc ttttgatagg tattttcttg accttcaacc aaaccagtag
    16141 cccaatgaac acagcgatat ccagccagta gatgacatca tggggcttca tcaaagaaaa
    16201 ggagcttcct gacaatcctt gcgacacttt cgaaaagcct aatacagatt tgactgtaat
    16261 gaaatctgtg aattcccgat aaaaaataat attcaagtac aacaaaagcg tatttaaaat
    16321 atcaatgatc aaaagaatag ctaacgctgg ctttggcttt ttaatataca aagaaaggcc
    16381 aaaaaacaat aaggtagtcg caattgggtt gatccacaag ataaagtact ggatcgtacc
    16441 ttccacacct aatgaaaaat ctgcatagta ggcaatgatc gtcttcagcc agaaaaagaa
    16501 aacagctgtc aaaacgattg ctaaacgatt ttggcacaat tttttaagtt gtgtcacgta
    16561 tatcacttct ctctattcac attttttctt attttgctcc tgaaaaatga aaatttccta
    16621 aaaaagttgt tatcttaaga tttttttatt ctttctaaag attattccgt ccacttttca
    16681 cagcataaaa ccgctgcgat gtaagcgatt aaactaatga agagataaaa gtcttgtggt
    16741 gaccgcttca ctccacggta ttcaaaagcc agtttctcgg tattcgtaca aaaaaagagg
    16801 ctgtgacaaa attcttgtcg cagctcctta tttcttgatg caggacagct ttttcacaac
    16861 ttcttcctta aacggtgttc tcctgccaac tcctcggcca caagtcacaa tatttataaa
    16921 acatgagaag ctgttttttg aaattgttcc ttgtggctca gagttaaacg gcaggctcaa
    16981 gacctcttga tacacggtgt tcaatcagct gaccctcggt attagattat gtcaggacgc
    17041 taattttaca acctcttggt aaacggtgtt caaaagcttg catctgcggt aataagccca
    17101 accaaacgaa aaatataagg agctattttt tgaaattgct ccttgtggct cagagttaaa
    17161 cggcaggctc aagacctctt gatacacggt gttcaatcag ctgaccttcg gtattagctc
    17221 aaaaaacggc aaaatatgag gaactatttt gccgtttgtt tctctaatac tcaggtcaaa
    17281 acgctgattt cacaacctct gtttttcatt gtccatagga ttgatattgt ccttgtgatg
    17341 actcattcgt aataattgag gcttgatttt cttcacttcc cgttatcgtt tgtccactgc
    17401 cagaatctga aggacttgtt cctgtcgact cgtttttttg agaagagaca gttgttttct
    17461 tgacttgttc atttgtcttt ttccattccg ctaaacgatc ggcgaccact tttttgtcta
    17521 ctttttcaga ataagcggtg atcacttttc catttttcaa acgggctact ttgtcgccat
    17581 catgagtcaa aagcaaaacg gtcaaatgct tgtctttaaa ttgatattgt ctgagagaaa
    17641 tcgcactaag tacgtcatcg atattaataa attgattttc ttcttttcct tttggcaaat
    17701 aaatgtccaa ttcgcctttt tcgtaattgg cagttatttt atactccgcc caccaataag
    17761 ttgagaattc ttttttgaaa ttatctactg attctttgct tgctacgatt ttttctgttt
    17821 ctttctgagc aagatctgct gtcatttttc ctgaaaatgc tgcggataaa ataagcagta
    17881 agaaaaaacc gaataaccaa aagagaagaa acggtctttt tttcctcatg ctttcctcca
    17941 ctcctattta attttgacta ttctattata tacaaaaaat gaaatagaaa gacaaaaaag
    18001 ttgaaaaaga tcgtaaataa ttgaacttct ttttcacaaa atggtaagaa tggagaattt
    18061 cacgcatttg ctctcaacta ttctttctta agcctcaaat ccgtttttat ctgatacgtc
    18121 tttgaaccaa tgacccgatt ttttgattgt tctttttcca tcttgtgcta agtctactga
    18181 aataaagcca tatcgattct tatatgcatt ggtccacgac cagttatcca tacatgtcca
    18241 catatgatag cctaaacaat tggcaccttc ttgaatggct tgatggacat atttcagatg
    18301 agattggaca aattcgatcc gataatcatc gtggatcatg ccatcttctt tgataaatcg
    18361 tgcttcgttt tctacaccca taccgttttc agaaataaag cagtcgatat ttccataatg
    18421 atctcgtaca ttgatcaaaa tatcatagat tccttgttca tagatctccc atccgcgata
    18481 aggattcatt ttacgaccag gcatttcata attgtcgaaa taatcctcag gcattggacc
    18541 gtttttagta tcgattggtg tttcttttgc ttttacccgg cgtggctggt aataattgat
    18601 acctaaaaga tctactgtat tttctttgat aaccgtcaaa tcctctgcat ggatcacagg
    18661 catgagatca agttctttta ctatctcaat caattcactt ggaaactcgc cttttacagc
    18721 tggatcaagg aatgaacgat tgaagaacgc atctgctatt tgcgctgctt taacatcttc
    18781 agggtttgat tcgtctcttg gatagcttgg tgtcagattc aagatgatac caataacacc
    18841 accttgctcc atttcatggt aagatttgat ggctaacgct gaagccaacg cttcatgata
    18901 gccaacttgg atcgcttttt tcatatctga ctcttctgga taatgccatc gatataaata
    18961 acctgcttcg actggaacga ttggttcatt atgagtaaac cattttttta ctttcgaacc
    19021 aaacaattca aaacaacttt tggcatattt ttcataggct tcaaccgttt ctcggttcaa
    19081 ccatccgcct ttttcttgca gatacatcgg catatcgaaa tgatacagat tcacaaaggg
    19141 ttcgataccg ttttcaatca actcatcaaa ataacgatga tagaaacgaa cggcttcttc
    19201 gttgattgtt ccagattcat caggtatcaa acggctccat tggatagatg ttcgaaatga
    19261 attatgccca gtctgtttca tcaactgtac atcctcttga tacttcgtat aaacttgcga
    19321 ggttttttca ggtcctactt gatcaaagaa cttttgcggt tcttttttgt accaataatc
    19381 aaaaatgttc tctccttttc catcgcctga aatccttcct tctgtttgtg ggccacttgc
    19441 tgcagaaccc caccaaaaat ttgctggaaa ttgatatttt ttcatcaaag cacctctttt
    19501 tcttttttag ttcgttttag ttgattccat gtatgatatt ttccatcaca atcacttttt
    19561 cattttcttc ctcttctttt ttgattcgtt ttaacagctg ttctgctgct cgttccccga
    19621 ttttatctgt tggtggcgtg atcgtgattt tcggaaggat actcagatcg tttttatcta
    19681 aataatcata gtgactgaac gcaatatcct tcccgattct gacaccttct tgattgaaac
    19741 attcgatggc accaatcgac atattgtaat tgctgacaaa aatagcagtt gccccttttt
    19801 ctaataatgc ttttgccccg taatagccgc tttttctaga atagtctcct gtaaagacct
    19861 ctaattggat cgatggatca ttttggaccg cttctttggc tcctgctaaa cgttctcttg
    19921 ctacgtagtc tgtttgagtg gcagcaataa atccgatttt tttatgtcct tgttcaccat
    19981 gatgagcgat cacttttgct acactttctc gatcattcac taatattgta tcaatacctg
    20041 ctggtccttc tggacaattc aatgcgataa ctggaatact catttttgtg agcatttcaa
    20101 atccagacca ctcttcttcg gataagaaga caatcagccc atcgatcaca tgcttcatca
    20161 ggtattcaat tttttgttca attttttctg gctgatcggc aaacccgctt aataataagc
    20221 tatacccatt ttcttcagct actctttcga tccctgagac aacttccgac ccaaaacggc
    20281 tggaaatccc gtttatcaaa agtccaatag agaagcttcg attattcttc aaccctttag
    20341 caatgatgtt ttctttatag tccaacgctg aaatcgcttc tgctattttt ttcatgttct
    20401 tttccttcag ttgctgacca ttcaagtaac gggaaactgt tcccactgaa acaccagcca
    20461 tcttagccac atcacggatc gatgccatac attctcactc ctgccagtta catttgattt
    20521 ctctttcttg ttgttcgatt atgatgaccg cattctttgc atagtgttgc ttgtcatcaa
    20581 tatccatata actaaagtct tcagtagagc aacttcctct cactattaat tatacttaaa
    20641 caagtatttg aaccggttca tttttaatat agcatttttt taaaagattg caacactttt
    20701 ttaaaacgct ttctgaaagt ggcggatttt ttgccttttt aaaattttat tctaaaattt
    20761 actctatttg tgggtgtttt gtgcgttttt ctcttccatc cgtttgaagt tttacattcc
    20821 aatggtataa tttctttatc ctatcaaatg ggggaagtat tttgaagaaa ttaatcgtac
    20881 tatttatttt gtctgcggga cttttgacat tgactggctg tgatttgttt ggcggctctg
    20941 accaatcctc ggacgaaaca accgattctt ctgtcttaaa agcggaaaaa gcagatacca
    21001 ccaacagtgt atatacgcta aaagtacgta aagatatgga tttatccgcg ggacagttga
    21061 catttacaga taaaaaaatg gaatggacac gcacgtatca gacacaagga actgattctt
    21121 ctgaatcatc tgaagaaaaa acagtgctga cagatatcaa gatcactacc aaagatgacg
    21181 aatatattat ttcaggaaaa gaaaataaaa aagaaacaac agtcatattt acaaaaatcg
    21241 gtaattaccg tttgaaggat gaggatggca acatctacag tctatagtgc cgtctgttgg
    21301 atcttctttt gtttctcccc ttcttttctg ctatagtaaa gaagcaagat gattgtaaag
    21361 gagacgaaat ggatgatcga tagtcctgat tattgtacat gtaataacaa caaaaatatc
    21421 catacagaat ttaatgacca aggaagcttg agcatatgcg acaactgtca caaactagtc
    21481 aaagattcat ttaaaacatt tgaatcttat cctgttcaaa attatgtaaa aagtagaaga
    21541 agataaacaa atccaagcaa aactcactat ggctgatttt tatgaacttc aaactcagct
    21601 tcttcctttg atcaagcttc atcagtaaaa caaacagtgg atcatttcag tttacaaatc
    21661 aaagagtaaa gatttattta tttcaaatat ttgaatataa aataaccaaa cgatagaaag
    21721 gcttattcta catcacgtag taatttttct ttcccgtcgt ttggtttttt atttttagcc
    21781 taattcttga ccttttgcat gctggttatc cgtaaaatat tttccagcag gctttccttc
    21841 caattcagct aatttttctt ggatctgacg gatcaccttt tttgcatctt ctggattttc
    21901 tacaaaatcg aatttatccc catccacttg tattttagga catacatcaa aatcttcata
    21961 ccattgatta tagcgggtgt tcaacgtttc ataataggta tataacgtct catcgctttc
    22021 tagctgttca tagtcacgcc cacgtttttt gatacgttct agcatcgtgt ccaaagaaac
    22081 tttgatatgg accatcaaat caggccgttt ttttggagca acatcatcta actcgtttag
    22141 catcgtatct aataagctat cgtactgctg tacttcgata tccgttaccc gacctaaatc
    22201 agcgtttaga tggaacaaca ggctatcttc atagatggat cggtctagca cattgtcatc
    22261 gttcactaaa gcatttttca ttgctaaaaa tcgcttgttc aaaaagaaga tttgcaataa
    22321 aaaagcgtac tgctcaggat ttgaataaaa gagcggcaat acttcgttat cttcgacatt
    22381 ttcataaaat ggtttagaat caatctcttt tgcaagcata tccgtcaaac tgctcttacc
    22441 tgcaccaatc gttcctgaaa tcaaaatcac tgacatacaa cccttcctct tctataagta
    22501 ctcgcctacg taagtttcaa caggcaatgt ttcaaaatca tattcacgta cagcttggtc
    22561 tgattttaaa tacgtatgga gactttgtgt aatcatcaag ttcgtaggta ccgtctcttc
    22621 ttgtacaatg atcatcggtt ttttcaacat agttgccaca cctaattcgt aagccgttcc
    22681 tggatcgatt gtttgatgct caaagtcaag gatagcaaca acaaattctg catttgtcag
    22741 ttcttccata tctttttcgt atacttcctg cgcccatcca gcgctgaata gttcataatt
    22801 gctttcttgg tgatgacgtg gagagtagaa acttgtgact gttttgtttt cctccaacgc
    22861 tttttcgata cgacttactc gatcgatttg ctcctctgaa aaaaatggtc ctgctaaata
    22921 tatttgactc attattttgc ctccttcaat aataactgat ttttaataga tactgctcgt
    22981 aattcattga ccagttccaa tggattgttg gaaaagatta cttgatccaa ccatttttta
    23041 tccataaaat cctcttttgc aaaagtttct aataattgtt gcaagccgct gtaataaccg
    23101 ccaatattca atattccgat tggcttgtcg ggcgtcagac cgattgctat ccaagaaaga
    23161 agttgtgaga attcctccaa tgtacctaga cctccaggga aaacgaggac agcatctccc
    23221 ttttcaaata ataatacctt tctttcatcg atcgtttctg taggaataaa tgtagtaaca
    23281 tcttttccag gcaattcagt ttcaaaaagc ccattcggat agatacctgt gacttttccg
    23341 ttattttcca gtactgtttt actaatcact cccataatcc cggagtatga accaccgtaa
    23401 accagttcga tttcttcttg tgccatgtat tttcctaacg tttgcgcaat aaatttgtaa
    23461 ctttctttat taccaaaacg ggaaccgcaa aaaacagtaa gtttcataaa aaaacactcc
    23521 ctaactcaaa agtctatctt cattctacac gtgttttttt aaaataaaag actagatttt
    23581 gtagtttttt agtggtatat aggatagacg ccactatata tagtatttag aaaaaaatca
    23641 tttt
//
LOCUS       DACDPS010000038        22680 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.038, whole genome shotgun sequence.
ACCESSION   DACDPS010000038 DACDPS010000000
VERSION     DACDPS010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.038"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..501)
                     /locus_tag="IWJ07_001578"
     CDS             complement(<1..501)
                     /locus_tag="IWJ07_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9333161.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFG"
     gene            890..2656
                     /locus_tag="IWJ07_001579"
     CDS             890..2656
                     /locus_tag="IWJ07_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAP9333162.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2758..4017
                     /locus_tag="IWJ07_001580"
     CDS             2758..4017
                     /locus_tag="IWJ07_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9333163.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            4164..5465
                     /locus_tag="IWJ07_001581"
     CDS             4164..5465
                     /locus_tag="IWJ07_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9333164.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            5468..6319
                     /locus_tag="IWJ07_001582"
     CDS             5468..6319
                     /locus_tag="IWJ07_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9333165.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            6332..7147
                     /locus_tag="IWJ07_001583"
     CDS             6332..7147
                     /locus_tag="IWJ07_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAP9333166.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            7298..8464
                     /locus_tag="IWJ07_001584"
     CDS             7298..8464
                     /locus_tag="IWJ07_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9333167.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            8694..9341
                     /locus_tag="IWJ07_001585"
     CDS             8694..9341
                     /locus_tag="IWJ07_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAP9333168.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            9425..10369
                     /locus_tag="IWJ07_001586"
     CDS             9425..10369
                     /locus_tag="IWJ07_001586"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAP9333169.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            10504..11955
                     /locus_tag="IWJ07_001587"
     CDS             10504..11955
                     /locus_tag="IWJ07_001587"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAP9333170.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            11976..12812
                     /locus_tag="IWJ07_001588"
     CDS             11976..12812
                     /locus_tag="IWJ07_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP9333171.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            12812..13471
                     /locus_tag="IWJ07_001589"
     CDS             12812..13471
                     /locus_tag="IWJ07_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9333172.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            13597..14286
                     /locus_tag="IWJ07_001590"
     CDS             13597..14286
                     /locus_tag="IWJ07_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333173.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            14292..15248
                     /locus_tag="IWJ07_001591"
     CDS             14292..15248
                     /locus_tag="IWJ07_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAP9333174.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            15292..15804
                     /locus_tag="IWJ07_001592"
     CDS             15292..15804
                     /locus_tag="IWJ07_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9333175.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(16060..16608)
                     /locus_tag="IWJ07_001593"
     CDS             complement(16060..16608)
                     /locus_tag="IWJ07_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9333176.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(16697..17878)
                     /locus_tag="IWJ07_001594"
     CDS             complement(16697..17878)
                     /locus_tag="IWJ07_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAP9333177.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(18114..19622)
                     /locus_tag="IWJ07_001595"
     CDS             complement(18114..19622)
                     /locus_tag="IWJ07_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAP9333178.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            19781..19972
                     /locus_tag="IWJ07_001596"
     CDS             19781..19972
                     /locus_tag="IWJ07_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333179.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            20175..21092
                     /locus_tag="IWJ07_001597"
     CDS             20175..21092
                     /locus_tag="IWJ07_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAP9333180.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            21085..22158
                     /locus_tag="IWJ07_001598"
     CDS             21085..22158
                     /locus_tag="IWJ07_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333181.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            22306..>22680
                     /locus_tag="IWJ07_001599"
     CDS             22306..>22680
                     /locus_tag="IWJ07_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333182.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLP"
BASE COUNT         7509 a         4044 c         4474 g         6653 t
ORIGIN      
        1 tccaaaaata aacagattaa gagaaacaac gaatgcgtct gtaaaaaata agccaatcga
       61 agtattcaag tggaaatatt ttttaaaaat gaggggaggg atcgtcgtac cgccgctaga
      121 agagttgttt ttatataaaa tcgcaactcc taaagcaaaa atcgcacttc caaaaatgac
      181 agacaacaac cgatctgaag taatcattgt ttctggaact attgcaatga ttaaaggaaa
      241 aaccaagctt ccgaaaagta ctttaaaaaa gggtttttta cctaaaaata gaagagctag
      301 taccaacata acgatattca acactaaaat aactatagct cggtcaaagc ctaaagcagc
      361 ttccagtaat attcctagac cgctgacacc tccagcagca atatgatgag gccctaaaaa
      421 catattgata gcgactccta atatgaagag ggcaaaaata atcttaatgt atgtccatat
      481 aaattgtctc gtttctttca tttattcact tccttaattc attaataata ctcttatctt
      541 attataactt acttttcacc ttaagttaac tttaattaat aaaaaaatta ggctagtcta
      601 aattattatg tttcattctt ctagaatata cttctacgcc aaaaactgtt aaaaaacttt
      661 tactttagaa aattggagaa attgatcgct taagataaat tcaggacact aaatagtaca
      721 ggaagtagac taaaactttt aaaagtttta agtctatcta tttggcaagt ttaaaaaata
      781 aaaattcggt atcaaaaaac tgttaccgat aacatttttg aaagcgttgt cttttgcttc
      841 tctatcactt atagtgatga cgtcatatta aaacacaagg aggaaatcaa tggatacagc
      901 tgcaatttat catcgaccgg aaagtgaata tgcatttcta tatacgaata caagatttcg
      961 tattcggctt cgtactagga aaaatgatat caaaaaagta tatgtgctat gtggagatcc
     1021 atataccata acaactgaaa aatggtatca aaaacaacga ccaatgaaaa aatgtctaag
     1081 taccaatgtt catgattatt gggaaattga agtgacgagc gaaacaagtc gcttacagta
     1141 tgcgttccat gttgttgggg aagataacac ggactgtttt tacggtgatc aaggaatttt
     1201 tccttatcga gaaaatgtga ttactgaacc aaatttttat tttcgaatac cttattttca
     1261 tcaaatcgat cgattcacta ttccagaatg ggtaaaagaa acagtatggt atcaaatttt
     1321 tcccgaaaga tttgcaaacg gggacaaagc aaatgacccc gaacacactt taccttgggg
     1381 aagtaaagat ccagaccgag aagacttttt cgggggagat ttgcaaggtg tgatggatca
     1441 tttggattac ttgacggacc ttggcatcaa tggcatctat ttttgtccca tatttaaagc
     1501 cacatccaat cataaatacg atacgattga ttattatgag gtggatcccg cttttggcga
     1561 taaaaaactt ttaaaaaact tgattgatga agcacataaa agagggatac gtatcatgtt
     1621 cgatgctgta ttcaatcata tgggtgtcca ttcaccacag tggcaagacg ttctcaaaaa
     1681 cggagaaaaa tcgatctata aagactggtt ccacatccgt tttttcccag tagattctta
     1741 tcagatgact gacttaccag aaactgctga aaatatccca tatgatactt ttgcttttac
     1801 tccattcatg ccgaaactaa atacagcaaa tccagaagtg caaaaatatt tgctggatat
     1861 cgcaacttat tggataaaag aattcgatat tgatggatgg cgtcttgatg ttgctaatga
     1921 agtcgatcat catttttgga aaaaattcag agaagcagtt acagaaatca aaccagacat
     1981 ctatatcctt ggagagattt ggcattcctc tcaagcttgg ctgcaagggg atgaattcca
     2041 tgcagtgatg aattatgcat ttacagattc gattaaagat tattttgcaa aaaagaaaat
     2101 cacagcttca caaatggtca gtggaatgaa tcatcagcaa atgctttaca gagatcaggt
     2161 gaacgaagga acattcaact tattagattc tcatgatact gccaggatct tgaccctttg
     2221 ccaaggtaac aaagaattga tgaagtctgt gatggccttt atgtttcttc aaaaagggtc
     2281 tccttgtatc tattacggga cagaaatagg gatgactggt gaagatgacc cggattgccg
     2341 caaatgtatg atatgggaga aggaggaaca agatctagag ctattcggtt ttataaaaga
     2401 acttgtttct ctaaggaaac agttgtctaa gattatttct gaaggttcta ctcagtggct
     2461 aatagtaaat gatcgtgaag ataagctcta tttcacacgg aaactagagg gacagatcat
     2521 ctatgtttac tttaatcagt caaaagaacc ttgggtagta gaacaggaaa atgaggtgat
     2581 tcttagtcaa aattgtcagt tgttagaaga cgggaaagca gagatacgac aatacggatt
     2641 tttgatcatg tgttgaaaac tgttaccggt aacaacaaga aagcgtttgc aaatgaagat
     2701 ttgtgcccat tataataagg gtgtaacagc acgttgaata caaaggagga aatgaagatg
     2761 aaaaaaagaa gtgtgaaaca gttgggttta gggttaatct ctttaggaat cttaggcgga
     2821 ttagcggctt gtggcaatgg taactcttca gcagaagggg aagccgatga aggaaaaacg
     2881 ttaacagttt ccgttgattc tggatataaa gattatataa acgaaataaa aaacactttc
     2941 gaaaaagaaa acgatgtgaa aatcaagcta gtagaaaagg acatgttcga tcagttggaa
     3001 tcattagcgt tagatgggcc tgctggaaag ggacctgacg ttatgatggc agcatatgac
     3061 cggatcggtg cgttaggaca gcaaggacat ctggcggaag taaaactggg aaatgaagca
     3121 gcttacgatg aaacagacaa agcccaagtg acttatgatg gaaaaatcta cggagaacct
     3181 gcagtaatcg agacccttgt tttgtactat aacaaagatt tagtcgatac agcaccatca
     3241 acgtttaaag atttggagaa tctatccaaa gacagtcgtt ttgcttttga gtcagaagca
     3301 ggaaaaaata caggattttt agctaaatgg actgactttt attattccta tggtttaatt
     3361 gcaggttacg gtggctatgt atttggtgat gatggcacaa atccttcaga tatcggtttg
     3421 aataatgcag gtgctgtaga aggaatctct tatgcaacag attggttcaa aaatgtttgg
     3481 ccaaagggaa tgcaagacat aaaaagtgca ggagacttcg ccagccagca attcatgtcc
     3541 aataaaactg cagcaatcat tgacggtcca tggcaagcgc aaacatacaa agaaaacaat
     3601 atcaattatg gtgtagccaa aattcctact ctaaataatg gacagcctta tcaaccgttt
     3661 ggcggaggaa aaggatgggt agtcagcaat tatgctaaaa acaaagacct ctctcaaaaa
     3721 tggcttgatt atgtaacgaa tcaggaaaat caagaaaaat tctttgaaat ggtaaatgaa
     3781 atccctgcaa accaacaagc tagggaaaca gcaaaggcta aaaacgatga attaacaaca
     3841 gctgtgattg aacaatatga aacagctcaa gcgatgccaa acatcccaga aatgggcgaa
     3901 gtttggacag gtgcagaaaa cttgatgttc gatgcagcat caggttcaaa aacaccaaaa
     3961 gaatccgcag atgaagcagt aaaaacaatc agtgaagcca tagaacaaaa atactgatta
     4021 aaatgataac cgtccatttt tataaaaaat ggaacaggtg cttcagataa aagtggcaag
     4081 agcgagatgg aaaattatct tgctcttgtt gccattatct taaaagcagt ttgcggttta
     4141 gcaagtttag agggggcatt cagatgcagc aagataaaac aagcaatatc aacgaaaaaa
     4201 aaatcacgct ttgttcactt attccaggga tggggcagtt tgtaaatggc caagctttta
     4261 aaggtatcgt ttttttcgcc atattcgtcc tattcattgg tcagatgctt ctgggcggat
     4321 ttcatgcatt ggtaggattg gttactttgg gaagtatacc aatggaagat cattcattat
     4381 ttattttgat tgaaggcact ttgcaattca tcattacaat cattttcttg attttttacg
     4441 gtttgaatct tttagacgct tatcgagtag cgaagatgcg tcaagagggc aaaaaaatca
     4501 atcggacgat gaaggaagtc atacataata taggtgatga aggttttcct tatctattga
     4561 cacttcccgc ttatcttctc atgattttta cgatcatttt cccagtccta gttacattgt
     4621 tcactgcatt tactaattat gacttcaatc atattccacc agccagttta attgattggg
     4681 taggggctaa aaactttttt agtatcttct ttttaagctc atatcgtaat actttttttg
     4741 cagtcttttc gtggaccgtg atttggactt tatgtgcaac aactttgcaa atcacccttg
     4801 gtattctcac agcggttgtt gctcaccaga aatttattaa attcaaacgt attttcggta
     4861 tcattttctt attaccatgg gcggtcccag cttttattac tatcatgagt ttttcgaata
     4921 tattcaacga tagcgtaggt gccatcaata ctcaagtgat tcctttcata aaccagcttc
     4981 cttttgtaga tatcagtgca atctcttgga aaacggaccc gaactggacg aaagtagcaa
     5041 ttatcttagt gcagggctgg ttaggatttc cctatatcta tgtgatggtt acaggaatct
     5101 tacaatcgat ttcagaagac ttatatgaag cagcaaaaat cgacggagca aatgcatggc
     5161 agcgcttttc aaacatcacg cttcctgctg tcttcctagt ggcagcgcca acatttatta
     5221 cacagtatac ggggaatttc aataatttct ccatgatcta tctgtttaac aatggcggtc
     5281 ccggaagcgt aggcggagga gctggttcta ctgatattct tatttcctgg atctataaac
     5341 ttacgactgg aacttctccg cagtattcta tggcttcagc aatcacgttg attatctccg
     5401 tcatcgttat ctgtgtgtca ttgctggtat ttaaaaagac aaatgcgttc aatatggggg
     5461 attaacaatg aaaaaatttc attcgagtgc tcgattccaa aaacgacttt ctcacttttt
     5521 tacttacttt tttcttttaa tactgtcagt cattatcatc tatccgcttt tgatcacagc
     5581 aagtactgcc ttcaagtcag gaaatattgc agcttttagt ttagatcttt caagtgactg
     5641 gactttagga aactttactc ggctattcag tgaaacactt tacgggactt ggtataaaaa
     5701 tacattgatc atttctgtcc taacaatgat cgtacaagtg actgtagtaa ctttggcggg
     5761 ctatacttat agtcgctatc gttttatcgg ccgcaaatca agtttgatgt tctttttagt
     5821 gattcagatg gtaccgacta tggcagcatt gactgccttt tatgtcatgg ctttcttatt
     5881 agaagcgctc gataagtact ggttcttgac aatgatctat ataggcggtg gaattccaat
     5941 gaatacttgg ctgatgaaag gatactttga cactgtacca atagatttag atgaatctgc
     6001 taaacttgat ggggcagggc atttcaggat ttttttccag attgtattac cgcttgtcaa
     6061 accgatgctc gccgttcaag cattatgggc ttttatggga ccttttgggg atttcatgtt
     6121 agctaagttc ctgcttcgaa cacctgaaaa tcagactgtt gctgtcggct tacaaacatt
     6181 tatttctgat gtgacaaacc aaagagtctc tctttttgct gctggggcaa ttttgattgc
     6241 actaccgatt tgtctattat tcttcttttt acaaaagaac tttgttaccg gcctgacatc
     6301 tggcggaaca aaaggatagg agcagagtat catgcaaaca tttccgttaa actatttttc
     6361 cgcattacgt acgccaactc aagtgtttgc gggacgcaaa ctattatcat ggcctaaatt
     6421 tttcttgata tttgtatttt tagtttcctt gatggtcatg cctgtgactc ttttttatgc
     6481 caatcagatc caagcgattc cattagaaca atttctatcc gttcattcgc tgatcgatga
     6541 acaaggaacc caaaaatttt ctgaacttga attatctgaa actgggttac aagcatcgca
     6601 acaaacgata gctgtgaccc ctgaaatttt ggtaggtgtg tctttatctg agaaacaaca
     6661 atccgatcat ggaactttta ttgattttga gaaggaacaa tgggtgatcc aacaaaagga
     6721 taaatctggt atacgccgct atacgatgaa ttatagccca tctttccaac cagattctgt
     6781 acgtacgccc gaagatttcc aacgattttt agaaagagaa ttttatgcca gtaatcgtcc
     6841 aacaatcatt ttatcttata gtttaagtct tggtcttgtc ctatttgtca tgacgagtct
     6901 cattttattc ggggcttcct ttttcttgtg gatgacaaga aaaagccaac tttcatccat
     6961 tcatacattc aaagagagtg caaatttgat gctgaatgtt atgggaatag gttccatggc
     7021 agcagcagtt gtagggttta tccactttga ctttatcttg atgctaagtg tccaaacaac
     7081 gataactgtc ttgctgcttt tatgggtttt tgtcaaaaca agattcaaag ataagcgtgt
     7141 cgaatgacaa gtttaagcga tttatgcgac aatgaatgtg aaaaaaatga aaaataaagt
     7201 agtttttgaa aaatttctga aaaaattaaa cttcttagtt gattcttttc ttttttagta
     7261 ttaacgtaat agggtagttt ttttaaggag gaaaatcatg ttagagaaaa ctttatacaa
     7321 tcaattattt tcacgttctt tctcgctccc tgtcgaagta aattattggg acggtacaac
     7381 gaaacaatac ggagatacgg acactcctcc ccaaatcaaa atcaatatcc atgaagaaat
     7441 cccaatgaaa gagattacaa gtaacgcatc tttggcacta ggagaagcct atatggatca
     7501 tcgaatcgag atcgagggaa gtatccaagc attgatcaac gatgcttatc agcaagcaaa
     7561 cagcttttta cgaggaaaag attatctaaa atggctgcca aagaaagaaa aacatacgaa
     7621 acaaaaaaac aaagaagata tccatgcgca ttatgactta ggtaacgact tttataaact
     7681 ttggctagat ccaactttga cttattcttg tgcttatttc aatacacctg aagatacgtt
     7741 ggaacaagca caaatcaata aagtccatca tatcttagac aaactcttta tcaaagaagg
     7801 cgaaacgttg cttgatatcg gttgtggctg gggaacattg atgttcactg ctaccaaaga
     7861 gtataatgtc aaggcaactg ggatcacact aagtgaagaa caatacgatt tgattaccaa
     7921 aagaataaaa gaagaacatt tagaagataa atgccgtgta ttattaatgg attaccgcga
     7981 actaaaaggc gaaacgtttg atcatatcac aagtgtaggt atgttcgaac atgtaggttc
     8041 tgaaaatctt gaaggttatt tcaaagtggt aaaagattta ttgaaaccaa aaggaacagc
     8101 tttgatccat ggaatcagcc gacaacaagg cggtgcgaca aatgcttgga tcaataaata
     8161 tattttccct ggcggttatg tccctggtgt tactgaatta gttgataata ttacaaaaaa
     8221 cgatttgcaa ctgattgatt tggaaagctt acgtcgtgac taccaattga cattagaaca
     8281 ttggacaaag aattttcatc aagtccaaga tcaagtcacg aaagaaatgg gtgattcctt
     8341 ctatcgaatg tgggatctat atttacaagc ttgtgcagct tcatttgaat caagtaatat
     8401 cgatgtgatt caatatttgc ttgttcaacc tggaaacaat gacatcccaa tgcatcgtgc
     8461 ataattgagg caaaagtact gtttaaagac tatttaaaaa gaagctaagt tttatgatgc
     8521 tccagaagtg aacttttttg tctaacttct aggattgtct ctaaagctta gtttcttttt
     8581 ctttatattt tcataataaa agtttggttc tttaattttt aaacatgaca gaaaattttt
     8641 tcttcgttat acttgtaata gaatactatt ttataacaaa ggggatcgaa tcaatgaaaa
     8701 aaactgttat ttatactagt ttatttgcag ctttgttttt agctgcttgt caatcaaata
     8761 ccaatcaagg cacatctggg aatacgtctt ccagtagcga acagcgctct tcacaggtat
     8821 cggactcctc aacaaaagca agcacatctt cgtctagcca gacaaaaagt tctagtgaag
     8881 caagtactca gtcagatatc aaagtgtctg cagctcgtgc gatcgaattg ttccaagata
     8941 aatacccaga tactgcaatt actagtttgg aattagatag tgattgggga tcttacttct
     9001 acaagataga aggtgtcgat gatcaaaaag aatacgaagc aagaattgat gctcaaaatg
     9061 aaaaaatgga gacagagaat ccagaaactc tagatcgaga tgaacaaaat ggtcaaaaga
     9121 aaaaagaaga tggtctggat gttgataaat tgatcacgat tgaggaagca gcaaaaatcg
     9181 ccgtcgacaa agtaggaagc ggtacggcaa cagattggga tttggataaa gatttaggta
     9241 ctacttattg ggaagtcaaa gtaaaagacg gcaatcaaag tacatcagtc aagatcaata
     9301 gccaaacagg cgaagtactg tcaactgaac aagatgatta agaatttctt cggaaaacaa
     9361 gagttgattt ttcttttagt catgatacac tagacaaaat aacttgtttg aaggagcgat
     9421 ttacttgagt ttagaaacga cctttgccaa tcatatcttt gccaatcctt tgatgaatgc
     9481 atctggtgtc cactgcatga ctacccagga attagatgaa ctggctcatt cagaagcagg
     9541 agcatttatc actaagagct gtacgatcaa tgaacgaaaa ggaaatcccg aaccacgcta
     9601 ttttgatgtt cctttaggga gtatcaattc tatgggactt ccaaacttag gtttttctta
     9661 ttatctagag tatgctcttg cgtatgaaaa agtgcaggaa aaccaaaatc aaccgttatt
     9721 tttttccatt gccgggatga gtgtccagga aaacttggaa atgcttgaga aaatagaaaa
     9781 aagtgggttt aatgggatca ctgaattaaa tttatcttgt cccaatgtgc ctggaaaacc
     9841 acaactagct tacgattttg aggccactta tgaaacactg aaagaagtat tcagcatctt
     9901 ttctaagccg ctaggaatca aattgcctcc ttattttgat tttgctcatt ttgatcagat
     9961 ggctgatatc ttgaatcagt ttccattgac ctatgtcaat gctatcaaca gtgtgggcaa
    10021 tggtttgtat atcgatacag aacaagaagc agtagtcatc aaaccaaaag aagggttcgg
    10081 cggcattggc ggagagtata tcaagccgac tgctttggcg aatgttcgcg ctttttatac
    10141 acgtttgaaa ccagaaatcc aaatcattgg tacaggtggg attcggacag gacaagatgc
    10201 atttgaacat ctgctatgtg gcgcaagcat gttacagatt ggtaccgagc ttcataaaga
    10261 agggccggaa attttttcta gaatcataaa agaattaacg cagattatgt cagaaaaagg
    10321 atatacgtca attgacgaat ttaaaggcaa attacgcacc atttcatagc aaagagacga
    10381 aggtcatgat tgatcttcgt ctttttttat aaaaaacact cgtctgttca ttcctgtgca
    10441 agcgtactca ttgtatgtta gacgaacttg tgggaaaata acagaggcaa aaaggaggat
    10501 aatatgaatg atgcaacaga attagcaaaa gagcttaaag aaaaagaatt tagttttcaa
    10561 gaactagtta cggatataca gaaaaaaata aagaagcaaa atcatgaatt gaatgcgttt
    10621 gtcgcgcttg aaccattcga gagtaattcc cacgagctat tgattcccca gtcagaaaaa
    10681 gggccattgg ctggtatccc atttccttta aaaatgctcg gtcaagaaaa aaagggatgg
    10741 ctagctactt caggatctcg tttatttgaa acacaccgag cttctcatac atctaattat
    10801 gtgcggcaag cagaagcgat aggtctagtt ccttttggac aaaccaatgc gccagagttt
    10861 ggtttcaaaa atattacaga cccagtcatc tatggcccag caagaaaccc ttggaacctt
    10921 gaatgtacac cgggcggatc aagtggagga gctgctgctg cagtttcatc aggaatcgtt
    10981 cctttagctg gtgctagtga tggcggtggt tctattagaa tacccgcttc gttttgcgga
    11041 ttgattgggc tgaaaccttc gcgagggacg atgccagttg gtccatatgc ttggagagga
    11101 tggcaaggag ctgctattga tttcggcttg acagtttcta tgagagacac ggaggcttta
    11161 ttttatggga tgagaagtat ccattccggt gcgccatatc aagtatcgcc agttgagtgg
    11221 caaacacatc caagaaaaaa acggttgaag atagcttttt gtgttgattc accgatccac
    11281 tcacagatta gtgaagaggc agtacttgct gtaaaaaaag ctgtagattt ccttggaaaa
    11341 gaagggcatg atttgacaga gattccttac ccactagatg gtcgtaaact gatcacgtct
    11401 tattatcaga tgaatggcgc agaaacagca gcaatgatcc gctcgattga aacaggcatc
    11461 cagcgagctg tcagaaaaga agaacttgaa cccatttctt ggactttgtt ccaatatggt
    11521 cagaagctct ctgccgctac ttacatcgat tctctgcatg tatgggatga agcagcaatt
    11581 gtcatggaaa acctacatca agaatacgat ctattccttt caccaacgac tgcatttacc
    11641 gcccctaaaa tcagcgaaga tttgcaaagt gatgcgataa gagcacagat ggaacacatc
    11701 agtgattgtt ctgaagtaga aggtttagac gttgtttctt ccatgtttga ggagagcttg
    11761 aagatcacac cttatacaca gttggctaat ctgactggac aaccagcaat cagcttgcct
    11821 actcatttga caaaagaaaa catgcctcta ggaatccaat ttatggctgc cagaggaaga
    11881 gaagatttgc tatttcaaat cggctatcta tttgaagcaa atcatcagtt ccatttacca
    11941 aatccatgga aatagcagaa acaaggagaa gaagaatgaa gaaaaaaaga ttgtttgctc
    12001 taataactag tgcctttttt attttaagcg cttgcggtac agctgccgct aatcaggaag
    12061 ataacagctg gagtaatgta gaaaaaaaga aagaattagt agtagcgact tctggcacac
    12121 tgtttcccgc atcttattat aatgatcaaa atgaattagt tggttatgat gtagatttgg
    12181 caaaagaggt ggctaaacgg ttaaatgtca aaatctcctt taaagaatac aacgtggatg
    12241 gacaaatcac ttctgtttca aaaagagaat cagattttgc tgcgaacgat tttggtatca
    12301 caaaagaaag agaaaaaaaa ttctcccttt cagagacttt caaatattca tttgacagta
    12361 tgatcgtacg aaaaaaagat gattcaggaa tccactcact tgaagatctt aaagggaaaa
    12421 aagcagctgg cgaacccaat actagctata tgcgtttagc ggaaaaacac ggagctaaat
    12481 tagtcacata tgacaatgcc acgaatgatc agtatttaag cgatgtagca aatggtcgaa
    12541 cagatgttat tctaaatgat tattatctgc agaaaatggc cgtatctgct ttaccagata
    12601 tccctgtaaa aatactggag aacgtttatt tcaatgaaaa cgaaagtggt tttttattga
    12661 aagaaggaaa tgatgctctg aaaaagaaaa tcgatggcgt attaaaagag atgaaagaag
    12721 atgggacgac aaagaaaata tctgaaaaat attttcaaac tgatgtttca gtcaaaccga
    12781 aagaaacgat ggctgagtcg gtgagtgagt gatgtacttt cctctaattg atttttccct
    12841 catgtggact tcacttcctt tcgtactgca aggcttggta tacacattag ggattggctt
    12901 cgtgtcgttt cttttaggaa atctggtagg gttgcttttg acagttttag gtcttttgga
    12961 ttggctgcct ctgaatgtat ttattcgttt ttatttatcg ttctttagag gcattcccgc
    13021 actagtcctg ctgtttctgt tatattttgg gttaccttat caattaagtg ccttaacggc
    13081 ttcagtcatc tgctttacga ttaccagcag tgcatttatt ggtgaaatct atcgaggatc
    13141 actagcaggc gtttcttcag gtcagtggga cgctggttta gcttctggtt tttctttctt
    13201 tggggtattg aaatatatcg ttttgccgca agcttttcga atctcagtac cggcactcag
    13261 taacgtagct atggatctag tgaaaggtac ttcgttagca gcaacaatca cagtgccaga
    13321 aatatttcaa aaagcaaaga tcataggcgg tcggacattc gattattttt ctctctatat
    13381 tttagttgca ctgatttatt gggtcatttg tatcgggatc gaaaaagtgc aacagtattt
    13441 agaaaaacgt tctatgcatc agtcaatctg aaaaatagaa atggtgctag cgtggctggc
    13501 accatttcta ttttttgttt cttgtatatt ctgcctagta ttttaaaacc atttcatgta
    13561 aaatgtaaat cgaaaatatg ttcggaggta gaaaacatga agaccccgct aaccatcgaa
    13621 atagaatcct cgttatacca atattgcatc gagcaaggcg gcttggttgt agaagaagtc
    13681 acgatgcctg atgaccaagg aattgtagat acgcttagct gcttgacaaa atttgatgga
    13741 agccgagaat ggcgatgcta tgagctgaaa gtgacaaaag cagattttcg ctctacagcc
    13801 aaactgtcat ttgttggaca ttataattat tttgttttac caagtaaact atatgaagaa
    13861 gtaaaaactg aaatccctac aggaataggt gttttagtgt atcgtccgta cacggttgaa
    13921 gaggagatgc ctgcctctgg tacatttctg attgcgaaaa aagcagcaaa gaaagagctt
    13981 gctgtaccgg aagaagcttt gacaaaccgc ttcatggctt ctttattcag ggaagtaaga
    14041 aaagcaaagc aaatggagcg aggcgtaaaa tattttccaa cagatcaatt gtacaaagaa
    14101 ttgaaaagac gttcagaaaa tcaagatcca ttttctaacg aacgatatta tgaacggttt
    14161 ttagaggaaa tagctagtca ggcaatcaat gagttgcaag aagaagttat cgcgcttcag
    14221 catgattatg aatatttgag gcaacaacag cagatccgtc ggttgcctac agagccattg
    14281 gagtgaaaaa tatgtactat ccttatttaa gaggaaaaca atttgaccta ttagcattaa
    14341 aagaagcact gtcgcgagga ttgctctcca ataaaatcca accagtgatc gaaccagtac
    14401 gtgattccgc tacgcttaaa aatgtgatcg aattatttca aaaaaagcac catcgaatcg
    14461 ctgtgatcca aaatccccaa gtaggacaat tcaaattatt cgatcagcat gtgcacagtt
    14521 ggacagtcaa agaaaatagc tctgtcgtcc ctgcacagat cttaacgcca gaaaatctca
    14581 gtgaagtact ggattcacct ccagcgtttc ttgttttcga tggacagcac tacccaaaag
    14641 atacagaggt gtggaaacag ttggcaggag ttgattcgaa atttttgata ccagatacta
    14701 gtcgctttcg catctggctg cccgaaaaca aaattgtgat ccgtgactct tttcaaacga
    14761 gaaagcatgt tgaaagttat gcagataaaa cagatgactt ttttagtgat gactatcttt
    14821 ttttccacgc agacggatat attggatttt ctgatttcac gatcgaaggt agccgttatt
    14881 tcgataaagg gttccctagc cgagcgcttg ctatccatct aacgtatatt gatgcttacg
    14941 gcaatatacg cgtgaaacat tttgtctctg acagtaacga ctctgctaaa gaccaagcat
    15001 tgaagtttct tgaagcagga gataaaatga aaaaatggat catgcgtcac catcatcaac
    15061 tcttgattac ttcaggaatg attgaattat tggctcttta ccagcaacaa aaattccctg
    15121 gactgggagt gttgaaaaag tggtcactta tgcatcattt ggaacttatt agtcacctgc
    15181 tagatcatcc aaaagattgg cttaaatatt attcaaaagt tcaggaacta tatgaagtga
    15241 tccaatgatt agataatgta aatgaaaaag gaatagaaaa gaggaaactg gatgtatatt
    15301 cggcaaacaa ggaaagaaga agtagaagaa gtggtagaaa tcattaatgc cgcagcggat
    15361 ttcattgctt cgcaaggctc tcctcaatgg caaaacgggc agaaaccaac aaaagaaacg
    15421 attttgaacg atattgaaag aaaagaaagc tatgtattaa tagtggaaga tcaaattgct
    15481 ggaacagctg cattagtctc aagcgtggat cccgtttaca caaaaataga tggtagttgg
    15541 aaagaatcaa ctggtcctta tttatcgatt catcgtgtcg ctttagcaga aagtactcga
    15601 ggaaaaaaac tagcaaaacc gttcttagaa catttgttga cgattgcagc cagcaaaaat
    15661 atccaagata tccgtatcga tacttatcca gctaatgaac cgatggaaaa gaccatctat
    15721 tctgctggat tcacttatca agggatgatt gaatttcctt ttgaacacgg cgaaagaaaa
    15781 gcatatcaaa taatcatcaa ttgactataa aatcatctga gcaaagataa agacattaac
    15841 actactgaaa aaaacaatca tatgatagcc aagaggttgt aacagaagtg gccagcttca
    15901 aaaaacaaga aagaatttcc gaaaatgctt ctcaaatttt tgtggaattt tagcgtattt
    15961 tacgaaatag ctgctttctg aaactccctt catcgagagg tcgtgacata gcttttgtca
    16021 cgacctctcg gcttatccta cgagtattat tcgatacatt tatatcaata atcctttttt
    16081 tattttatct gcagcttctt ggaacgctcc atcgtttgac acgactaaaa gtgaggggat
    16141 agctttcaaa cgtttcaact gtgctaaatc ttctacataa accgccagtc tggctgaaat
    16201 ctgttcagat gaatctccac gttttttcag gcgcgattgc aatgtttctt tagagatcgt
    16261 aataaagata ggacggacta ccgtcccgaa atactcatat aaatgccaaa aaccatttgc
    16321 tgtcaaaacg gcgtaacaat ttcctactgt cagtttcttt ttaatttctg ctttactgga
    16381 accatattta tgttgagcat attggtccca ctctgcaaac tcatttccct tgatcatctg
    16441 attgaatgta ttgtcagaaa caaaataata atcctcttga tctttttccc caggtcttgg
    16501 tgatcgattc gtgtaagtaa tgatcttttg ttccggctga aaaatagctt ctgcgagtga
    16561 agtttttcca gaaccagaag gaccgataaa tacatagcaa taattcattg tcatttctcc
    16621 tttgatgatt gaacaagtga aaaagctttg gaaaatattt ccaaagcttt taaaagtaac
    16681 tttgcttttt tgtatattat accagcaagc cagtcacaat agcacttaaa aagctgacta
    16741 aagtcgctgt tagcaaaatt ttcaatccgt gttttgcgac aagatttcct ttttcttcat
    16801 ttaatccctt catcgcacca gaaatgatcc cgatagaaga gaagttggca aatgaaacga
    16861 ggaagacaga tataattgct gttgtacgct cagtaaaatg gaaattacca tttgctaatt
    16921 ctgtcatagc aacaaattca tttgtaacca gtttagtcgc catgatacta ccggcatcca
    16981 ctgcttcttt ccatgggatt ccgctgatga aggcaagagg ggcaaagatg taacctagga
    17041 tctcttggaa agtgatgccg aatatcccgt ggaagatagc attgatcatg gcaatcaaag
    17101 caacaaaacc gataagcata gcagcaaccg tgatcgccac atggaaacca tcaagaatgt
    17161 attctcccaa catttggaaa aatgtctgtt ctttgttttc ttcaatgatc agctcatctt
    17221 ctttttcttc taatacatag ggatttacaa ttgaagccag aatataaccg ccaaataagt
    17281 tcaaaacaag ggcagtgatc acatatttag aatcgatcag tgccatataa gatccgacga
    17341 tcgacataga aactgtagac atagccgata cactcatggt gaacaaacgt ttctcagata
    17401 ggaagggtaa ttcctttttt atggaaataa aaacttccga ttgtcctaaa atagcagaag
    17461 ctactccatt ataagattcc aatttgccca tcccgttgat tttacttaat cctagaccaa
    17521 cagctttcat aaataatgga agaatcttga tatagcgtag aataccgata atagcagaaa
    17581 taaatacaat tggcatcaat acggaaagaa agaaaggtgc acttccttta tttgctacac
    17641 cggcaaaaac aaagtcaaca ccttgcccag caaaagcaag aagatgatca aacatttttg
    17701 ccaatacaga aacaaccgct gtaccaaaag ttgtacgtaa taatacgaaa ccaagaacaa
    17761 gctgtaaaac gaacatcaca attagtggtt tcaatttgat tgcttttttg ttactgctga
    17821 tcaaaaaagc aagccctaat gtgacaaata aaccaataat acctaataaa taactcaaat
    17881 aatcactcct attccaaatt ccgttttatt ttacctgttt tgcaaataaa atactaatct
    17941 ttagtgaaat tttcattact tttttagagt gaaacgcatt cattttccat tagaaaataa
    18001 agcgaacaaa ataaaaggtt taaaaagtgt aacgtcttag taaagtctat aaaaaaacga
    18061 aactatcgat taagctaata gtttcgtttt caacatttat aagtaaactt tttctattgg
    18121 taatatagat tttcggaagg gtagacaggt gcgcaggtaa tatcgattcc tagttttttc
    18181 aatgtttgtt catcattttt attgaccatc acagtagagt gggcttgtgc tccctcaagt
    18241 tcagctagtt tatcataagc aacttgagcg attggattcg ttactgcact gattgataaa
    18301 gcgatcaaga cttcattgat gcttagtgca tccaaagagg aatgaagtgt ctttgatttc
    18361 aatccttgta ttgtttcaag aactaatggt gacaataaag gaatgtcatc tgcgatatgg
    18421 gctaaatgtt ttactgcatt taagataact gccgctgacg catccatcaa tgaacttgta
    18481 cgcccggtaa tgatgacttc atctggtaat tcgattgcaa tcacagcaga aggatctttt
    18541 gtaggacttt gcgcttgaat cttatctgca tagtcatggg cagggactac cacgtttcgg
    18601 tcttcaggac gtaaaccgac ttcttccata atcagtttga ttcgattaac tgcttcttcg
    18661 tcgatcaatc cttttttgaa gtcgcattct gtttggaaat aacgacggat gatttcttgt
    18721 ttagaggctt cttggacaac ggtatcatcc acgatcccaa atcctacacg attcacaccc
    18781 atgtcagtag gagattgata aacagattct ttaccagtaa ttttttcaat aatacgtttg
    18841 attacaggaa atgtctcaac atcccgatta tagttcacag ctactttatt gtaggcatca
    18901 aagtggaagg aatcgatcat attgacatct tttagatcga ctgtggctgc ttcataagca
    18961 atgttcagcg gatgtttcaa cggaacattc catactggga aagtttcgaa cttggagtaa
    19021 ccagctgcat gtcctttttg gctttcatga tacagttggt tcaaggaagt agcgagcttt
    19081 ccacttcctg ggccaggagc tgtcactaca acgattggtt tagttgtttc aatatactca
    19141 ttttttccaa aaccatcttc tgagacgatt ttttctacgt tgactggata gtcttcgatt
    19201 tctgagtgtt tatagacctt gatgtttcgt ctttctagtt tattgataaa gactttcgtg
    19261 gcaggctgtc cactatagcg agtgataacg acactgttga ttaataagcc ataaccacgt
    19321 aattcatcga ttaaccgcaa gatatccata tcataagtaa tcccataatc accacggatc
    19381 ttattacgtt caatatctcc agcatagacg caaatcagta tttcagcttg atctttcaat
    19441 ttttgcagca atttgatttt cgcatcttct tcaaatcctg gaagtacacg tttcgcatgc
    19501 ttgtcgtcaa ctagcttccc accaaattct aaatataatt tgtcatagtg gttcactcgt
    19561 tccaaaatat acgctgactg ctcttcaata tatttttctg aatcaaaacc aatttttttc
    19621 actaggttcc ctccttaagt actaaagcat tttttcattt ttttgaggat tcgtcaactg
    19681 gaagagcgaa attcctcaaa aaaacaaaga aaatggttta aaccaacttg tctagatagt
    19741 tattcattgg tatgatagtt tttgataagg aaggtgctac atgaaaaaaa gacagaaaaa
    19801 gaaaaacgca tataaacact atatccgttc gatttttacc ggttatgaaa gaatgcttga
    19861 agatcccgaa ttggaacaat tgacgtttac ttatctaaac gaagaaacgc agctaactcg
    19921 tgatgagcat cagcgcattc atttcacaac aagagatctg cctagtaaat aaaaaataag
    19981 ataaaggtca tcggattcga ttctgtccta tcagatggaa agactagttg atcgaaaaaa
    20041 aggctgtacc aaattttggt gcagcctgtt ttatggagca ttgcccgctc ttttttataa
    20101 caatttttta cagaaaatgg tactattgaa gtattaataa gtgaaaaaca gatgagtaag
    20161 aggaggcaag tagtatgaaa gagccagata caaatgcaaa ttatgagcca gacgaactag
    20221 aggagacgaa tgcggatcag gatacaggac aagaatcgtc ttctttttac gatatcaaag
    20281 aaaaagcaag catgaatctt tcccaattat tagaagactt acaggctttt gaaaaagctg
    20341 tccgcgaaga agatatccct gagatctacc gtttatacaa tggccgattg aatgaagaat
    20401 taaaaagaag ctcaaatgaa aatcacgaga tcgatcaatt gctgatgcgt aagatccatg
    20461 atcgtttttt acaggaattt ccttttatgg aacaagtaga aaacatctcg ccaacgatga
    20521 gctattacaa aatcggcact tattaccatg atcggcccac ggtcggtatt gatgcaagtc
    20581 tgccagaaat ttttgttttg cctaagatcg atgaagagtg ggagaaatat tcccaaccgg
    20641 agtcaacgga ctatgacaaa aaaatcaatg agctagacgc aaaggtgatc actgctcaga
    20701 cagaaattga acgtttagat gaacaaataa aagaaatcaa tcgacaaatg actgatttga
    20761 acgacaataa aggattcttg aatcgtaaaa aaatcgagga agaaattcaa gaattagaga
    20821 agaagaagca agtactatct aatgaaaaat taggctggct tccttatatt gagacgcctg
    20881 aaacaatcca gcagcaaaaa gaagcattga agcaagaagc aagagcagac cagcttcgtg
    20941 cagccatcgt agaaaaagaa caaaggcaaa tcagacgcta tttcggtagc aaagagggat
    21001 tcggtcaagc aatccatgaa ttcttgatga attatcttgg caatgagaat cctaataatc
    21061 aatctaatga aggaggttcc gagtatgaat gaacacgatg aagcagaatg gacaaaaaat
    21121 gaagaacaaa cagaagatca tgaagaaacg aacacttcat tggaagaaca aacaagtaat
    21181 cctctggatg acacagaaga aatccctatg ctagaggaaa caaaaataca cgaaaaagaa
    21241 aaagtaactc cgacgaatac gaatactgca gaaaacaccc atgcttacga aataactgaa
    21301 aatcccgggt atttagaagg aaaagagcaa gatcatcaag atatcgtcag tttgattgaa
    21361 acagcggaag cagaattagc ggatttacgt atgagaaaag atttgatcga atcagatttt
    21421 ccacaattat tagaatcata tgatcgtttt atattgaatg aagaaccaat ctccacgatc
    21481 ggaaagacaa gcctgatcga atacttcacg tatgaattac tgaaaccgtt gaatgatatt
    21541 ggtctggaac ggtcaaattc agcttatgac acatggaaaa caagacattt ttcgatcaat
    21601 tatacgttga atggacaaaa cgaattagtc ttttcctttg tattaccgac catcgatcaa
    21661 cgataccaag tggaatcgat gagtctccta aacgtatcac cagagacaat ggaaatcaat
    21721 gtccaagatg accgggtgct ttctttgatc cgacactggt cagtggatcg agtattctct
    21781 gcagggcaga tcacgatatt caatcacaaa ttgaatcaat tattagccca tgcaagaaca
    21841 cttggtttta ccgttaatca gacattacta gacaatacga agccacttcg tttgaatctt
    21901 cagagcgaat ttgaattgac gaatgaagta ttggatgata tttttatcgt ggcaatgaaa
    21961 aatccatcct acgatatgga aaagatcaac gaaactactt atcaagtacg attagataaa
    22021 ggacagtctt taacgataac aacaaacgaa aaagatcaaa cagtcttgtc gatctcatct
    22081 ggagactacc cacgatctgt cattgatttc tttatcaatt acgaattctt ggtcccatta
    22141 atggtgagaa agtcctgatt ggataagttg gattaccaga tgtcattcag ataattctgt
    22201 tccaaaggac ttgcggcagt tatccagaat aattgtcaca agtcttttct gtatgtacta
    22261 aatggacgtg aataacttgc tgatgaagga gggatttcat taggaatgtc cacaaataaa
    22321 atagcacagc ttagcttatt gtctgctgct tgtgtagcag gtaggatcgc ttttcaattt
    22381 attccgaatt tccagccaat gactgctatt tttttattta tagtacttta cttaagctta
    22441 aaagatgcgt taattgtcat gagtctttct attgcgatca gcagttttta ttttggtgtt
    22501 ggaccatggg tcatcggcca atggatcagc tatactgttg ttctgagttt gttttcaatc
    22561 gtctgcttga gaaaaacagt gcaaaggaat ctatggttaa agggagtctg tttttttctg
    22621 gcgggcatgg tttacggaat aggtatgacc gtttttgata cgctgttata tcgtttgcct
//
LOCUS       DACDPS010000039        21216 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.039, whole genome shotgun sequence.
ACCESSION   DACDPS010000039 DACDPS010000000
VERSION     DACDPS010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21216
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.039"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            28..546
                     /locus_tag="IWJ07_001600"
     CDS             28..546
                     /locus_tag="IWJ07_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WHG domain-containing protein"
                     /protein_id="HAP9333183.1"
                     /translation="MSVFELPTVTLIPNGIHHPPIEDGRLLPNSVKNMDDLRQALFEQ
                     IYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLCLEEYGGGKEMQNFSYTY
                     FVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSIIHPTDEQIEKMIQDSID
                     AILKFKEPIEID"
     gene            complement(724..1164)
                     /locus_tag="IWJ07_001601"
     CDS             complement(724..1164)
                     /locus_tag="IWJ07_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAP9333184.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            1322..2086
                     /gene="map"
                     /locus_tag="IWJ07_001602"
     CDS             1322..2086
                     /gene="map"
                     /locus_tag="IWJ07_001602"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAP9333185.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            2118..3041
                     /locus_tag="IWJ07_001603"
     CDS             2118..3041
                     /locus_tag="IWJ07_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAP9333186.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            3117..4256
                     /locus_tag="IWJ07_001604"
     CDS             3117..4256
                     /locus_tag="IWJ07_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAP9333187.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            4249..5049
                     /locus_tag="IWJ07_001605"
     CDS             4249..5049
                     /locus_tag="IWJ07_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9333188.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            5049..5876
                     /locus_tag="IWJ07_001606"
     CDS             5049..5876
                     /locus_tag="IWJ07_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9333189.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            5854..6588
                     /locus_tag="IWJ07_001607"
     CDS             5854..6588
                     /locus_tag="IWJ07_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9333190.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            6688..7554
                     /gene="rfbA"
                     /locus_tag="IWJ07_001608"
     CDS             6688..7554
                     /gene="rfbA"
                     /locus_tag="IWJ07_001608"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAP9333191.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            7568..8140
                     /gene="rfbC"
                     /locus_tag="IWJ07_001609"
     CDS             7568..8140
                     /gene="rfbC"
                     /locus_tag="IWJ07_001609"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAP9333192.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            8162..9190
                     /gene="rfbB"
                     /locus_tag="IWJ07_001610"
     CDS             8162..9190
                     /gene="rfbB"
                     /locus_tag="IWJ07_001610"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAP9333193.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            9288..10139
                     /gene="rfbD"
                     /locus_tag="IWJ07_001611"
     CDS             9288..10139
                     /gene="rfbD"
                     /locus_tag="IWJ07_001611"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAP9333194.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            10174..12207
                     /locus_tag="IWJ07_001612"
     CDS             10174..12207
                     /locus_tag="IWJ07_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333195.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            12251..13531
                     /locus_tag="IWJ07_001613"
     CDS             12251..13531
                     /locus_tag="IWJ07_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAP9333196.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            13741..14547
                     /locus_tag="IWJ07_001614"
     CDS             13741..14547
                     /locus_tag="IWJ07_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9333197.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            14559..15779
                     /locus_tag="IWJ07_001615"
     CDS             14559..15779
                     /locus_tag="IWJ07_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9333198.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            15769..17355
                     /locus_tag="IWJ07_001616"
     CDS             15769..17355
                     /locus_tag="IWJ07_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9333199.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            17394..19532
                     /locus_tag="IWJ07_001617"
     CDS             17394..19532
                     /locus_tag="IWJ07_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9333200.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAESTHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
     gene            complement(19900..20883)
                     /locus_tag="IWJ07_001618"
     CDS             complement(19900..20883)
                     /locus_tag="IWJ07_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HAP9333201.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
BASE COUNT         6695 a         3586 c         4403 g         6532 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaaaatatgg atgacttgag acaagctctt tttgagcaga tctatgatca tttacaatat
      181 gaagtgttca ctgttgagca tacagggaat acgattatcg atcttgcttt taattatatc
      241 cattttgcaa gaagagagaa gaagttgtat cgttcactgt gcttagaaga atatggtgga
      301 ggaaaagaga tgcagaattt ctcttatact tactttgtga atacagtgaa acgcgatcca
      361 gcttatgtga atctttcaga agagcagatc gaatctttgt ataatgggac atggatcata
      421 gctactggga tcgcagcttt gatgtcttca agcatcatcc atccaactga tgaacagatt
      481 gaaaagatga tccaagacag tatagatgct atcttgaaat tcaaagaacc aatcgaaatc
      541 gattgaatat actcaaaagt tccacaaatc agtaggtaac aagagatgtg tcaaaataag
      601 gaaaagttcg tttttcttac agcgtatgca tttttaactc ccgaagaact cttccatgaa
      661 tgcaatgaat aaaagagcca tacagattga ataaatctgg atggctcttt tattgcaagt
      721 atattaaatt tttgcgacta gttctttagc gaaagcttct aaattttcga tatcttcttc
      781 ttcagcattc agatccactt taacattttc ggctcctttt gatgcgcctg tttttgcaaa
      841 agcagcatcg aaatcatcca ccgatttgca gaattcatcg taaaaagtat caccagatcc
      901 gcagacaccg taaatttttc ccaatagatc caattcttgc aagtcttcat aaaagtctac
      961 gatctcatca ggcagatcgc catcatcgta tgtataagta gctactacgc aaatatctgc
     1021 ctcctcgaat tcatctgcat ctacttgggt acattcgttg atttcgactt cgatatccag
     1081 attttcaaat gcttctgcaa caatatcggc gatttcttca gtgtttcctg tcatgctagc
     1141 atatacgatt tttgctaaag ccatgatgtg cctcctcgtt tataattatt taacaattat
     1201 gaattatacc aaaacatcat tgaaaagaaa ataaaaccta aagacacctg gggaatgtca
     1261 tttggctaaa tgtatgccgc tatgttaaaa tgagagggat ttgtaaaaag gagacgaaaa
     1321 tatgattact ttaaaatcag cacgtgaaat tgaaatgatg gcagaatctg gagctttatt
     1381 agcagatgta cataaaaatc tgcgtgattt tataaaacca ggaatcacaa gttgggatat
     1441 tgaggtattt gtcagaaact atatcgagag tcatggcgga attgctgcgc aaattggctt
     1501 tgaagggtat gaatatgcga cttgtatcag tataaatgat gaaatttgtc atggattccc
     1561 tcgtaaaaag cctttgaaaa acggtgattt ggtaaaagtc gatatgtgta tcgatttgaa
     1621 aggcggcatt tctgattctt gttgggccta tgtagttgga gaatctacac ctgagatcga
     1681 ccgattgatg gaagtaacga agaaagcttt gtacatcggt attgagcaag cacaagtagg
     1741 gaatcggatt ggtgacatcg gtcatgctat ccaaacttat gtagaaggcg aaaatctagc
     1801 gatcgttcgt gattttattg gtcatggcgt tggtcctacg attcatgaag aaccagcagt
     1861 tcctcattac ggagaagctg gaaaagggtt gcggttgaaa gaaggcatgg ttatcacgat
     1921 cgaacctatg gttaataccg gaacttggaa aatgaaaatg gatcctaacg gctggactgc
     1981 gtatacaaga gacggcggac tttcttgcca atatgaacat acattggcta ttactaaaga
     2041 tggtccaaga attttgacat cacaaggaga agaaggcact tattgatcta tccagtcaat
     2101 aggtacggga gaggtacatg aaagtttgga acaaaataaa acaaaatgaa aatctgatgc
     2161 gctttattga gacgactcaa aagcgcatca ctgattcaga aatgagtacg acctctgtcg
     2221 tcgttgctta ttatttactc ttgtctttat ttccattgat tattgctttt ggtaatattt
     2281 taccgtttct ccacatcgat caagagactg tactgaccta tttacgagaa gtgattcctg
     2341 agaccgttta ccagtttatc ggtcctgcaa tcaaagacct tttgacacaa agttcaggag
     2401 gcttgttatc cgtttctgca ttagcggctt tatggtctgc aagtcaaagt atcaatgctt
     2461 tgcaaattgc gatgaataaa gcgtatggcg tagaaaatag aaaaaatttt ttgatcgtcc
     2521 gtttcttctc actaattgtc attgtcttgt tcatgattgc tattagtggg gtaactatcg
     2581 ttcttgggtt gggacagctg attttggatg ctattcagcc aatcgtacaa atacccgtga
     2641 actttatcga tcagttccaa actttgaagt ggccaatcac attggtagct ttatttgtca
     2701 tcatgttctt gatttattta attgtcccta atgctcagtt gaagttgaag acgatcattc
     2761 cgggcacgat cttcgctact gtcggctgga tgctgttatc acaagtgttt agtatctatg
     2821 ctaggtattt tgcaacgagg atcagcggtt atcagatcat aggaagtttt attgtactca
     2881 tgctttggct taattttgct gctacgatca tcattttggg cggaatcatc aatgcagttg
     2941 ttcaagaata cgtgacgggt catcaaatca aaaaacgtcg agatccaagc cgaaaatggt
     3001 tcaataaatt gaaaactaaa ttttctcata aaaaaaagta gaaacaagag ttcttattgt
     3061 aaatttatat cagtaattta taataattat gactcttaat taaagtaggt gcccatatgc
     3121 ttgtttcttt tagtatggtt ataagatttt ttctgacaat tttattatca ctgatattaa
     3181 cacctgtatt caaaatcatt tcagtacaaa caggtatggt cgacaaacca aatgaaagaa
     3241 gaataaataa agttccaatg ccttcagcgg gcggactgcc tatctttatt tcatttgtta
     3301 tatcgacttt attcttattc cgtaaaatta tccctcaatc gtatattgta ccgatcttga
     3361 ttgcttcttt agtaatcata gtcacaggag tgctcgatga taaatatgag ctgacaccta
     3421 aacaaaaaag tatagggatc ttgattgctt ctcttattat ttattttgta gcagatatac
     3481 gtattgattc cttcacgctt ccttttattg gatatatcca actaggctgg cttagttttc
     3541 ccgtaacgat tttttggatc ttcggtatca cgaatgcggt caatctgatt gacggtttgg
     3601 atgggctggc ggcagggatt tcattgatcg gtttaattac gataggaatc attggatatt
     3661 ttttcctcca tgcttctaca gtgtatattc cagttgttat tttctgtctt gtagcaagta
     3721 ttatcggttt ttttccttac aatttttatc cggcaaagat ttatttaggc gatacaggag
     3781 ctctattcct agggtttatg atggccgtcc tttcactaca agggctgaag aacgtgactt
     3841 ttgtttcatc gatttctttg ctgatcgtca tgggagtgcc ggtaacagac acgttttttg
     3901 ctatcattag gagaaaagca aaccgtgttt ctttttctac agcagataaa aaacatcttc
     3961 atcaccgact aatcagttta ggctttactc ataaaggtgc tgtcctgacg atttatgcga
     4021 tggctttgat gttttctttc acagctatgg tcatgaatta tactggaaga atcggaacga
     4081 tcatattgat tattgccatg ttgtttgctg caattttatt atttgaattg attggtctga
     4141 tcaatgagaa acatcagttt attttaaaaa cattgcgatt tttagggaat aaagagtacc
     4201 gtacgcaagt aaaacagaaa tatttgaaga aatggaaccg tcatatagat gaataacaaa
     4261 ttaaaccagc cgatccttat tagtattgtg acggcaaaca gtgataaaat ttttcggaca
     4321 ctagataatt ttatttcaac tgtaaaagat gaaacagcag taaaaatccg aatttttgac
     4381 aacaattcac ttcccgagta caaaaaaaga ctaacagagt ataggaaata tccctacatc
     4441 gatatctatt ttcatgatga aaattccgga tttggttatg gacataatca caatctgata
     4501 gctagcgatt ctcgctatgg cattatttgt aacccagata ttttagtgga tgaagagaca
     4561 gttacagcat tggtcttgtt attgaaagaa catccggaat gtgcgatgtt agcacctaag
     4621 attcttaatg aagatggaac aactcagcac ctcatccgtg agcagctggc tgtttttgat
     4681 tacgttttgc gctttgtgcc tttcaaatgg gtcaaaaaaa agtttgacaa acgactggct
     4741 gtgtttgaat gccgaggatt gcctactgat gctaatagtt atgtacggat gatctcaggt
     4801 agttttatgg tagctgatat tgataaattt aaagagataa aggggtttga tgagcgtttc
     4861 ttcatgtact ttgaagacaa cgacttgtgt ttgagttttg aaaaagcagg ttacaagtta
     4921 ttgtacacac ccttttatgc agttatccat ttttacggga agggtgcgca tcgaaatgca
     4981 aaactgtttc gcgtgttttt gacttctatg atgaaatttt tcaataagtg ggggtggcgt
     5041 tttttctgat gaaaccagaa atattagtag tcgttgtgct ttatcagcaa ctattcagtc
     5101 aatctccagc ttatggacca ttaagcaaag cacttagcga aaagcaaatt cagcttatta
     5161 tttacgataa tagtcctcaa aggcagcaaa atgagctgtt tgaaaaagaa aatatcgttt
     5221 atcatcatga tccgcaaaat cctggtttag cggtggctta taattttgcg ttgtctcaag
     5281 cagatgaagg aacacgttgt ctcgtcactc tggatcagga tacgaggcta gtcgacgatt
     5341 attttgaact attgcgtaaa gtaaccttta cagatgaatg tgttgctgca gttccgatga
     5401 ttttttctgg gagccgacaa gtttctcccc tatattcaga tcgttatatc aatcgtacag
     5461 ctcaagcagt agaagttgaa ggcccaacag ctgaacgaat catggcaatc aattcaggtg
     5521 ttgcctggtc tgtcaaattt ctaaaaaaga ttggcgggtt taatcctgct ttttctttag
     5581 attttttgga tcattggctg ttttggaaag tggatcagtt ggaaaaaact gtggagatcc
     5641 ttcctgtacg aatggaacat gatctttcgg ttttagacta tgaaaaagtt tcaagtaatc
     5701 gataccgttc tattttacaa gcagaaaatc gattttatca tgaatatgat caaaggcatt
     5761 tatccagtca ccgacgacaa ttattgttaa gaacagctaa acagttcttg accgtcaaaa
     5821 accggcatat ctggcggatg actttgcgct catatgttaa gaattggaag gtgtgaattc
     5881 gtgcgttcgg tttgtatagc tacctttaat ggaggcagat atttaagaga gcagttagat
     5941 agtattttat ctcagctttc agcagaagat gaagtagtca tttctgatga tgggtcagta
     6001 gatgatacat gggatatatt aactgaatac gcagaaaaag ataagcgaat ccagcttttt
     6061 tcggggcctc gtcaaggatt gatcgctaat tttggttatg cgatccagca gacgaaagga
     6121 gatctaatct ttttggcaga ccaagatgat gtttggttgg agacaaaagt gaaaaagatc
     6181 actgactatt ttgagcagaa tccggatcag ctagtagtag tttctgattt aatcattgtg
     6241 gacagcgagc tgcaagagat ccatgattcg tatttttcgt ttcgaaattc caaagaagga
     6301 tggcttaata atgtgataag aagtcactat attggtgctg gtatggcttt ccggtcttct
     6361 ttaaaacccg atattttgcc gatcccgtca gaagtgccga tgcatgatat gtggattggt
     6421 ttgctgggag gaaagaagac tgggttccta agagaaccgt taactctgta ccgaagacat
     6481 gatttaaacg cgagtgagat tgaaacacat tctaaattac ggcaaaaaat ttcttggcgt
     6541 gttcatctga ttacggcgtt gatcaaacga aaatggttta aacgttgata aaagatgttt
     6601 aaatccttaa tatttccatg acaaatataa caattgatga gataataaca aagtgaaatt
     6661 tgaaatcaaa aaggagagca gatatccatg aagggaatca ttcttgctgg gggcagcgga
     6721 acacgtctgt accctttaac aaaggcaaca tcgaaacaat tgatgccgat ttatgacaaa
     6781 ccaatgattt attatccaat gtctactctg atgttggcgg gaatcaaaga aattctgatt
     6841 atttcaacgc cacaagatac accacgtttc aaggaattat ttggaaacgg aaatgattta
     6901 ggactgcaga tcgaatatgc agtacaagaa agtccagatg gattggcaca agcgtttatc
     6961 attggagaag aatttattgg agatgatagt gtctgcttgg tattagggga caatatttac
     7021 tatggtggcg gtctgtctaa aatgttgcaa cgagcagctt caaaagatac aggcgcgaca
     7081 gtatttggct atcatgtcaa tgatccagaa cgctttggtg tagtagaatt tgatgagaat
     7141 atgcaagctt tgtcgattga agaaaagcct gctcagccaa aatcaaatta cgctgtaact
     7201 ggcctttatt tttatgacaa tgaggtaatt tctatcgcta aaggtatcaa accttcagaa
     7261 cgtggagaac tagagattac ggatgtcaat aaagcatatt tggataaagg aaaactatcc
     7321 gtagaactta tgggacgcgg atttgcttgg cttgatacag gaacacatga atcattgctt
     7381 gaagcatcta cctttatcga aacaattgaa aaacgtcaga acttaaaagt ggcttgtttg
     7441 gaagaaattg cttaccgtat gggctacatc gataaggaac aattgctggc ccttgcacag
     7501 ccattgaaaa aaaatcaata cggtcaatat ttattgaatt tggccgctga ataaggagtc
     7561 cttacagatg aaagtaacag atacaaaatt aacagatgtc aaaatcgtag aaatggacgt
     7621 ctttggcgat catcgcggtt tcttcacaga aagttattca aaagcaaaat ttgcagaaca
     7681 tggattgaaa tatgactttg ttcaagacaa ccattcacta tcagctgaag ctggtgtcct
     7741 tcgggggctt catttccaaa aaggagaagc tgcacagaca aaattgattc gggtagtcac
     7801 tggagcagtt ttagatgtag tagtggatat ccgcaaaggt agcccgactt atggtcaatg
     7861 ggacggattt atcttatctg aacataatca tcgacagttg cttgtaccta aaggatatgc
     7921 ccatggattc gttactttga caccaaatgt gaacttcctt tataagtgtg ataattacta
     7981 taatgcagaa gcagatgggg gcatcgcatt tgatgatcca gatttagcga ttgattggcc
     8041 aattgatcca tcaagagcga ttatgtcgga aaaagatcac cgtcatccga cattaaaaga
     8101 atttgaagca gaaaatccat ttgtttacgg agaaatctaa gaattaagga gaaattttct
     8161 tatgaaaaac atcattgtta ccggaggtgc cggattcatc ggctctaatt tcgttcatta
     8221 cgtggtaaac aatcatccag aagtgcatgt cactgttttg gataaactga cttatgcagg
     8281 aaacaaagag aacttggccg gacttcctag cgatcgcgta gaattggtcg taggagatgt
     8341 tgcagatgca gaattagtgg accgtctagt aaaagaagcg gacgcagttg ttcattacgc
     8401 ggcagaatcc cataacgata attcattaaa agatcctttc ccatttgtac aaacgaatat
     8461 cattggtacg tatacacttt tagaagcttg ccgcaagtat gatgtacgct atcatcacgt
     8521 atctactgac gaggtttacg gcgatcttcc attacgtgaa gacctgccag gacatgggga
     8581 aggcgaagga gaaaaattca ctgcggaaac accatataat ccttcaagtc catattcttc
     8641 tacaaaagct ggttcagatc tgttagtaaa agcatgggtg cgttcttttg gcttacgtgc
     8701 aaccatttca aactgttcaa acaactatgg tccttatcag catattgaaa aatttattcc
     8761 tcgccaagta acgagtatct taagcggtat tcgtccaaaa ttatatggcg aaggaaagaa
     8821 tgttcgtgac tggatccata cgaatgatca ttcttcagca gtgtggctga ttttgacgaa
     8881 aggcagaatt ggcgaaacct atctgatcgg tgcagacggt gaagaagata ataagaccgt
     8941 gatggaattg atcttagaaa tgatgggcca accaaaagac gcatacgacc atgtaaacga
     9001 ccgcgcaggg cacgatttgc gttatgcaat cgattcgaca aaattgcgag aagagctagg
     9061 ttggcagcct gagtttacga acttccgtga tgggttggct gacacgatca aatggtacga
     9121 agaacatgaa gactggtgga aaaaagaaaa agaggccgtt gaagcagcat atgccaaaaa
     9181 tggacaataa tacagaggaa atttcactgt attaatggaa aaacacatgt gaggcaggcg
     9241 ctcgcctgtc tcttttttaa aataaaaatc aaggagcgga taaccatatg tttttattaa
     9301 ctggaggaaa tggacaatta ggtacagaat tgcgccattt attagatgaa aaaaatctgc
     9361 catatgtttc aacagatgca aaagacttgg atatcacaga tgctgagaaa acaatggctt
     9421 atattacaga attaaaaccc gatgttattt tccattgcgc agcttacaca gcagtagata
     9481 aagcagaaga agaagcaaaa gaattagatg agaaaatcaa tgtcgatggc acaagaaatg
     9541 tagcacttgc agcaaaagct gtgaatgcac aattcgtcta tatcagtaca gactatgttt
     9601 tcgatggaaa aaacaaaaat gtagaatacg aagtagacga ccaaacgaat ccactaaatg
     9661 aatatggtcg tacgaaatta ttaggcgaaa aagcagtaga agagatttta gaagactatt
     9721 atatcatacg aacttcttgg gttttcggga tctatggaca caatttcgtc ttcacgatgc
     9781 aacgcctagc ggaaacccgc gacaaactga ctgtagtaaa tgaccagttt gggcgaccaa
     9841 cttggacacg tactttggca gaatttatgg tatatgtcat cgaagaaaaa gcaccatttg
     9901 gtatttatca tttgtcgaac gaaaatagct gttcttggta tgagttcgca aaagaaatat
     9961 tgaaagatac agatgtagaa gttgcacctg ttacgtcaga agaatttccg caaaaagcaa
    10021 cacgtccgca gtattcagtc atgagcctga aaaagacaga agcgttaggt tttgtcatac
    10081 caacttggca agaagcactt gcgcaaatgc tggaagctgc caaaaaaatg aataagtaat
    10141 ttccttttta tcaaatagaa tttggagcat gatatggaaa aacaaacaag aagtgcacga
    10201 cgaaaagaaa agccaaaagc gaatcaggca gattcgaatt tggtgcttga cctgtttcta
    10261 aaactccgct atcttattgg cgttttagtc atcgtggtca tcgttacttt taatttaaac
    10321 ggaagttcac ttggtgtttg ggataaatat gtgtcgcaaa gagacgatgg gaaaaaaact
    10381 gatgtgattt ttggacagaa tcgggaagtg cggtcagacg aatggctggt gcagacacct
    10441 ttttacctct cacaagctga agaggacttt tcgttggtga acgaggatta ttcattaaac
    10501 ggacaaaaca tgatcattgc gtataattct cctgtaaagg atattacggt tatcggaaaa
    10561 ccattcaact ggggattctt tttcctcgga agagatcgag gactttcctt ttattgggcg
    10621 atgaaattag tcgtcatggt cttgcttgga ttcgaagtac tgatgatatt aacgaagagg
    10681 aaaaaagctc tatcgctaat cggtgcattt gcgctgacat tttctccagc agtccagtgg
    10741 tggttcatgc aacatgtggg cgatttgatt ttctttactt tgggactgat gattgctttt
    10801 tatcattatt tctacaatca tgataaaaaa tggctacgaa cgttgatgat gcttttggtg
    10861 gttattttcg gtattgggtt tattttagtg atttacccag cccatcaagt gatgctggcg
    10921 tacttgctga ttttttattt tgttggcctg cttatttact atagcaagaa agtttcatgg
    10981 gatctgttgg atgtagtgtt gattggaggg gcagttttat ttattggcgg agtcatggtt
    11041 catttctggc taacgtcaaa agacgcattg ttagcttctt tgaatacctt atatccagga
    11101 aaacgggtaa gtactggagg aaagtggacg attggcgaat ttttttcttt ccttacaaat
    11161 tggaaaattc cttttgaaga tatcaactat tcgaataact ctgaagtggc cctgttctat
    11221 cacttttttc caactgtatt cctagcaagt ccatttgtgt tatttggcaa aaaagatagc
    11281 gaacaaaagt tactgggacg tattttgatg attttttgtc tgttttctat cttttggatt
    11341 accgttgggt tgccaaaatg gctagcagaa atcactctgc tgtcttatgt tcctccatta
    11401 agagccatcc ttacttttag ttttgcggct tgtttattat cgatctggtt tatcgcttat
    11461 atttggagag aagatgttat tcctatttgg tataaattca ttttgttggc agtaaatgca
    11521 tggtcttatt tctatgcgat tacgcattcg aaaatggata attatttttc ggattttgaa
    11581 accatcgcag ttgctgtgtt gggaacagtg attttagctt ttgcattatt tggctggaga
    11641 cgcttgtttt acctcgcttt tgtcgcctta gttatcgtat ctggcttttt cgtcaatcca
    11701 gtcgtagaag gtacaggagc gatttttgaa aaaacactgg caaaagaaat ccaaaaaatc
    11761 gatcaacaag atcctggtaa agtttggttg tctgaagatg aattatataa cttcacccca
    11821 acattgggag tgaaatcctt caatacagta cgcttttatc cagatatagc tgcatggaag
    11881 aaaatcgatc cgaaagaaga atacgagaag tattataatc ggtatgctca tacaagagct
    11941 tttatcgcta aggaagatac aaagttcatt ttagatcgtc cagatatgtt ttcggtcacg
    12001 ttgaattttg aagacgcaga aaaattaggg atcgactatg tgatcagtaa acgaccatta
    12061 actgattaca accaattata tgaagcgcag ttcacgcagc tatatggacc ggataaagac
    12121 ggttatatga tttttaaagt agaatatccg gattatccag atgtaccaca aacacaaact
    12181 caggaaaatc agctagtcgt acagtaatag gctagctgag aataatcctg aaatattagt
    12241 agaaaggaga gtgaagataa ttcaagcaat cgtacaaaaa tttgtcaaca aaatgagtgt
    12301 cggtttcttg cttttgttgc tgacgctagt cttttttgtt tggacagcag gggtaggttt
    12361 tccagcaact ttgtttatat ataacaatag ttcattactt atcgctttta gtgtggctat
    12421 tttgctcatt ttaaatgttt ataaagcaaa aggcgtcgat tttctttatc taggtgttgc
    12481 gcttgccctt ttcattttct tttcttactt tcgggaatta agaaatgagt ctttatttgg
    12541 agacttactg tttcctttga ttattttatt ttgtttagta attaaatgga tcgagtggac
    12601 aaaatgggat cgcggtattt atctgattgt ctttacggtc ttcctcggaa tcacgttata
    12661 tcgtgtattt acagagatcc aagtaccaga aggacaaagc atctgggctc caggcaattc
    12721 gctttctgat atttggatca atacgaatac aatcggaagt tctttgatga ctcttgcttt
    12781 attgatcagt ggatttgcca gttcttttga acgatggtat gttcgaatca tcggtgggat
    12841 agcagtcgtt gcagcctttt taggtatttg ggtctgccaa tctagaggtg cattagtcgc
    12901 gttgattgtt tttgcgatat tggatctttt acctaaatcg ttgatgcgtg tcattcgtgc
    12961 gccatttatt gcctacacgg ttacgattct tttagctttg ccgatttctt atctggcggc
    13021 tgtctctgaa aaagtcaatt tgtttaccga tcgagaagat atttggcata aattctatca
    13081 gacgctagga gaaaaatctg aacaagtttt actaggaatg aaaacattta tcttccagcg
    13141 aggaaaccaa tttttaggaa accacaatag ttataattct attttgaata tctatggatt
    13201 gataggattt gggattgctg ctttgctgct tatccttttt attggtcgct taacgttgaa
    13261 agcagattta tccaacgggc agatgacttt tatctgggcc ttttttgcgg tgatgatgca
    13321 gtcatttatg gaggatacat tgacttcata cccatgggtt ccgattattt atatcctgtt
    13381 ggctatggcc gttcatcgat atgatgaacc agaagttgcg gaggaattct atgttatgga
    13441 agaggcatat gacgatatga cttatgatga tacatcttat gaggaacaac cagatagcgc
    13501 tcattcttcc aggatccaac gctatcattg ataggcacaa aatacgagag aggctgtgac
    13561 aaccgtcgca gccttttttg gtttattgca attgtctagc atcttctgta aaatagaaac
    13621 gttagagaaa atcttgtatc atagaaaatt aagaaagaaa taagaacgca gctcggtacc
    13681 aaaaatatgg taaaatggca cttgactgta attcactgaa aatcgcttgg aggaacacaa
    13741 atgtttaaag atttgtattt atttatgaag gacatttatc agaacagaaa attattgcta
    13801 cagttttcga tcaatgactt caaatcaaga tatgcaggat cgtttttagg aatcttatgg
    13861 gcgtttatca atccgctatt tactgtattg atttattggc tggtatttgg tgttggactg
    13921 aaagcacaaa tgatcgatgc aaaataccca tttatcgtat atttagttac cgggatgatt
    13981 ccctggttct tcttctcaga tgcatttgca tcaactacgg ttgtttttcg ggaatatact
    14041 tatttagtaa agaaagtcgt attcaatatt cgtatcttgc ccacgacgaa aatcctatcc
    14101 aatctgtaca cccatttatt cttcattctg attggcatag ctgtcgcgct tataaatggt
    14161 atatatcctt cagcaatgtc tttacagctg atttattatt tattttgcat gtgtgctttc
    14221 ttgacaggat tgacttggct gacggcatcg atccaaccat ttttacctga tatcatgcaa
    14281 ttaattacga tcgtgctgca gttgatcatg tggacattac cgatattatg gagccctaat
    14341 cagtttgatt caggtcttgt aagcatattg aaattaaatc cgctgtacta tgttgttcaa
    14401 ggttatcgtg agtcattttt aagtgaagct tggttctggg agcatggaac atatactttg
    14461 tatttctggg ttttcacgtt agtaatgttc gtaatcggct ctgcagtgtt ccgtagattg
    14521 aaaccacatt tttcagatgt gctgtaagga gaatagctat gacagaatat gcaattagac
    14581 taaaagatat cacaaaatct tataatatgt acgcaaaacc ttcggatcga tttcgtgaag
    14641 ccctcaatcc ttttaagaaa tcctatcatg atgtatttta tgcgttgaaa aacgtgaatg
    14701 tagaaataaa aaaaggtgag atgatcggtt tcgtgggaga aaacggatcg ggaaaatcta
    14761 caatgttgaa aatcattacg ggagtactga caccaacatc cggcacgatg gaaatcgaag
    14821 gaaaaatctc agctttgcta gaactaggat caggattcaa tcctgagtat tctggctatg
    14881 aaaatatcta tttaaatgga atggttctag gattttcaag agaagaagtc gatgaaatgg
    14941 tcgatgatat cattgaattt gctgacatcg gagaccatat ttatcaacct gtaaaaactt
    15001 attcaagcgg gatgttcgtt cgtctagctt ttgctgtagc aatcaatgtg aatcccgata
    15061 ttttgattgt ggatgaagca ttggcagtag gcgatcttga atttcaacta aaatgtatgg
    15121 aaaaatttac agaaatcaag aactcgggaa agacgatttt attcgtttct catgatatca
    15181 gttcgattcg tcgtttttgt gatcgttcct attggttgaa aaacggagaa gtcgtcgaat
    15241 atggcgatac gatggaagta acgacgaatt atgaaaactt tttaaagaaa aaatcagtta
    15301 aaacagtgga tcggaataaa aaccaagtag aacggttcgc cgctccagat atcgtcgacg
    15361 tgacgaaagc ggaattgttg gataaagaca aaaatccggt ggatatgctg gaacaaggag
    15421 aaaaactata cgttaaagta acttatgaag taaaagatga tacaatcaaa aatccggttt
    15481 taggaattgc gctacgaaca gtcgacaatt tttatgtatg cggacttaat actctactag
    15541 atggtatcaa gattccttgg aaaaaaggag agaatatctt ttatctggag tatgaaaagt
    15601 taggtttgtt agctggtgaa tattattttg acgttgcggt ctttgaagaa aatgcaacgg
    15661 ttcctttggt atataagaca aagtacatga acttgtttgt cagcggttct tatatcggag
    15721 aaggcatcgt ggtcttagac cataaatggg aagaagggac tcatagcaat gaaatatgat
    15781 tttgaaatgg acctagatga acaaagcagt gtgggaaaaa ttgccgcaca gatcaaacca
    15841 ggaagtaaag ttttagagtt tggtcccgga aacggccgat taacgaaaca tttaatcgga
    15901 gcaaaacaat gtgaagtgag cattgttgag ttagataagg aattgttcga ttttgtcagc
    15961 gaatttgctc aagatggatt ttatggtgat atcgaaagtt ttgagtgggc aaactattat
    16021 gctggacaga catttgatta cgtcttgttt gcggatgttt tagaacattt ggtagatcct
    16081 ggaaaaacat tgaagaaagt cagagaattt ttgaatgaag aaggagagat cctgattaca
    16141 ttccctaact tggcgcataa ttctgtcatg atcgatttgt tcaataacca attgccgtgg
    16201 gcttcttatg gtctcctaga tgaaacccat aattcttttt atactcatga tggcttccaa
    16261 aaagtttttg aaaaagcggg attattcatt aatattgaag attatttgta cttagctgta
    16321 ggtgatacag aattaaaatc aacttatgaa gaattgccgg aagctgtacg ctatgatttc
    16381 aaaatgcgac catttggtga agtctaccaa tactttttct cattgatgaa acatccagta
    16441 gcacaaagtt ccattgcaga gccacaaaat tctaattatg taaaagtgtt agaagtaacc
    16501 cagcaaacga aacaagatga aacaatccaa caaataccat tcaataattt cacaggggaa
    16561 aatgagacac taagtttccc cgtatcagaa acaacagaaa gaatggtgtt tcgcttcgcg
    16621 caacaaccta gctttatcga attcagcgca gaagctgccg gagaaaaact aacatttatc
    16681 gattcgaatg ctgtagtcaa aacagtaaat gattgttatt tgtttgatgg gaaagaatta
    16741 ccagagttcg ttctaacgga tatttctggg aaagaagtga ctatccattg tcactatcgc
    16801 tttatcgggg aattgacacc aacaatgaaa gaattgttgg aaacgattcg tcccatggct
    16861 gaagtcacaa agcagctttc tgagaaaaat gacgaattag aaagagaaaa tcatcatctt
    16921 cttgaggaaa atacaagact agaccatacg ttaaaaacaa ccacaaatcg ctattgcaca
    16981 ttgttggaaa gtgatgaatg gacaataaaa catagactag gacgacgtaa gaaagaaact
    17041 tcgaaaaaaa tccaagaaaa agaactttct atatgtattg acgagaaaat ctgggatgca
    17101 gaaacaaaaa tattaaaaat catcggatgg ggaatcgcta attcggatcg ccagcccttg
    17161 tcctataagt tatcagctga ccaatcgcca tttttcgaag caattcctgt ttcacgagaa
    17221 gaagtcaatc aagcagaaca gctcgcaaaa ggaacagaag cgggatttga gttacgaatc
    17281 ctttgcgagc aagaacgttc attcttagtg gaagcagtag ctcaaaatgg ccaatcatgg
    17341 atcattgaaa tgtaaataat gattaactta taaagataag gagtaaacga atcgtgcaaa
    17401 aaaaagaaat tgccgtattt atcgatcaaa tcactcgtga acgggccact ggcgatttgc
    17461 ttattgtggg ctgggcaatt gatgaagtga ccaaagaaat ccctacgata aaggtagaaa
    17521 aagaaaatgt gattgctgaa tcaacacatg tcgtaagatt ggatatcaat catttatata
    17581 atcttgatgt taagacccaa agcggtttca aggtacgttt atcagggaaa atgagaggaa
    17641 aagccatctt agacttccaa acagcgagac atcaaaatgg catagctgtg aaattaaacg
    17701 gtaaataccc ttatgatgac ggcattgaat cttcatggga aagaaaaaaa agattactga
    17761 aaaaagggat aaattatgca aggactcatg gcgtaaaaaa agcgatccgt cgggtcaaat
    17821 tggaattgaa gccaggatcg atcgactatg cagagtggat cagtcgtcat gagaaaccag
    17881 atttgaaaga gcaaaggaaa gaagcacaag gatttgcgta ccgtccattg atttccatac
    17941 ttatgcctgt ctataacgtt gaaatcaaat ggctcgaaaa atgcattgat tcggtactgg
    18001 atcaaacgta tgatcattgg gaactatgta tcagtgatga tgcttctact gatccagcta
    18061 tccgaaagtg tctggaatcc tatcaagcaa aagatgatag aatcaaagtc gttttccgaa
    18121 aagaaaatgg acacatcagt ctggcaacta attctgcatt ggaaatagca gaaggagaat
    18181 ttatagcact cttagataat gatgatgaat taccgccatt tgcattatat gaagtggcaa
    18241 aagtgctaaa cgtacatcca gaactggatg tgatctacag tgacgaagat aaaatcgatg
    18301 cagatggcaa tcgctttgat ccgcatttca aagcagactg gtcgcctgat acattgatgg
    18361 gaaataatta tatctctcac ttaggcgtat atcgaacaag cattgttaaa gaacttggcg
    18421 gtttccgtaa aggctatgaa ggttctcaag attatgattt ggtccttcgt gtgacagaac
    18481 aaatccctgc agatcatatt taccacattg accgagtctt gtatcattgg cggacgatcc
    18541 caggatcaac tgcaagtaat ggcgaagcca aaagttatat ctatgattcc ggagtgaaag
    18601 cattgacaga tgccctgtct agaagaaaca tcaaaggaag cgtacgtcct ggcagaattt
    18661 ctggatttta tgagattgct tatgacgtct tgcaagagga tttggtcagt gtgatcattc
    18721 caacgaagaa tggctatgaa gatctaaaaa cttgtgtgga ttcgatcatc gaaaaaacgt
    18781 cttattcaaa ttatgaaatc attattgcgg ataacggaag tacagatcca aaaatgcaag
    18841 aattatttgc agaatacaag catcagctga aagaccgctt tatcgtagaa ttgattgaca
    18901 ttccattcaa ttattctcga attaacaatt tagcagcaga aaaagcaaac ggcaaatact
    18961 ttttattctt gaataacgat acagaagtga ttgagccaga ttggatgacg gtgatggttt
    19021 cttatgcaca gtttgatcga atcggctgtg taggagcaaa actgttttat ccagatgata
    19081 caacgcagca tgcaggagta ttattaggaa tcggtggtgt tgcagggcat gcattgaata
    19141 actatgatcg aacacattgt ggttatttcg gccgattggt cattgacgtc aattacttag
    19201 cagtcactgc agcttgtatg atggtcaagg cagctgactt caatgctgtt aacgggtttg
    19261 atgaaacatt agaagtagca ttcaacgatg ttgacttgtg cttgaaagtt tatgaactgg
    19321 gtcgttacaa tgtctatgca catcaagcag aattgtacca tttcgaatct aaatcaagag
    19381 gttatgaaga tactccagaa aaacaaagac gttttgctgg tgaaatcaaa aaaatgcaag
    19441 ataaatggcc agcatatatt gcgcatgatc cattttacaa tgataattta acaaaagacg
    19501 gaatcggcga cttttcgttg agaccagatt aaactataat aaaaagctgt tatgttgtga
    19561 ttcgctcgca cataacggct ttttatagtt ttttgtcaaa aaaaggcaga aggtgccgac
    19621 tcaaaaaaat aagtcgaggt tgggacagaa gtgtttaact ccgagaaata agaagaaatt
    19681 cacgaaaatt gcttttcaaa tttttgtgaa tttcggctta tttaagaaga agttgcttct
    19741 gctcccaccg tttattcgtt tttaaagaat gagacaaaag tgtttcttac ttttgtccca
    19801 cgctcttatt catgctcaga gcgccaatag atacaaaatc atagccaaag tttcacgaac
    19861 cctagctgtc aaagaatcga tattttattt taatataggc tattttaaaa taaatggttt
    19921 aaggtattcc tctacaaaag ccactctatc aattatcatg ggtttaaaat aagaagtgat
    19981 ggagataacc agattgttct tagcgtcaat ataaagaaca tttccactat taccaattgc
    20041 tgaatatatt tcgttctcag aatctaatac ccaccataaa tagccatatc tcatattttg
    20101 taagtcttct aaagcactgg ttctaggttt gaacatctcg tttatccaca tgggtggtac
    20161 gatttgtttt cctttataca tccctttatt taggcataat acaccaattt ttgccagatc
    20221 acttgccgat aaacacaaac catatcctgc cgtcccgata tttagtggat cacaaaacca
    20281 gacattggtt ttaggagact tatccattgt aaactgctta tgttcttcag ctgtttcagc
    20341 ataataattg acatggtttg gtatccctat aggatcaaat agatacttat tggcaaaatc
    20401 aacagttgtt agcttacttg tctgatacaa caagccagtt aatatgtgta aacagacagt
    20461 ttgatagttg aattcgccgg ttataccttt tcttcctccc aataaatcta gtgatgtttt
    20521 tgtccaatct tcacttgaac agacttttgt ccatggatcg cctttacatt tgtagggtgc
    20581 tttcattgtt agcaagtgct tgattgtaat agcgtaaata gttttctccc cgcgtttcac
    20641 tttatactct ggaaaataat ctaatacttt atcatcgata cttttgatta atccttgctc
    20701 taaagcaatg ccaatcagca aagatacaat acttttcgtt gctgacatga tatgacaagt
    20761 atcgtctttt ttataaccat tccattcatc tgtataaact tctaccccat ccttatatgc
    20821 gacaatttga catatatttg gttgtcgaaa accaataaaa tcatgcaact cttgtttatt
    20881 cataagatat tacctcactt tcttctataa gcataatgtc attaaaaatc gatttcaatt
    20941 ttttcgattc ttcacgtaat acaggtattc ttatggacta gacagcgctt tgggaagatg
    21001 gattctctga aaaatataaa ggttcacttt atgagtggat aaaaaaagca gcaaatactt
    21061 cgacttcttt aatttaccaa cattagtgaa aatcgattca tctggagatt tgtaatttag
    21121 agtccttgtc ttgattagag gtgtataaag ctttttatta gacattttta aattccatta
    21181 caatttgctt ataaactagg tttttaaagg tgacta
//
LOCUS       DACDPS010000040        20999 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.040, whole genome shotgun sequence.
ACCESSION   DACDPS010000040 DACDPS010000000
VERSION     DACDPS010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.040"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..132)
                     /locus_tag="IWJ07_001619"
     CDS             complement(<1..132)
                     /locus_tag="IWJ07_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP9333202.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
     gene            542..1090
                     /gene="hslV"
                     /locus_tag="IWJ07_001620"
     CDS             542..1090
                     /gene="hslV"
                     /locus_tag="IWJ07_001620"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAP9333203.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            1104..2504
                     /gene="hslU"
                     /locus_tag="IWJ07_001621"
     CDS             1104..2504
                     /gene="hslU"
                     /locus_tag="IWJ07_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAP9333204.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            2522..3313
                     /gene="codY"
                     /locus_tag="IWJ07_001622"
     CDS             2522..3313
                     /gene="codY"
                     /locus_tag="IWJ07_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAP9333205.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            3395..4270
                     /locus_tag="IWJ07_001623"
     CDS             3395..4270
                     /locus_tag="IWJ07_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP9333206.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(4402..5040)
                     /gene="plsY"
                     /locus_tag="IWJ07_001624"
     CDS             complement(4402..5040)
                     /gene="plsY"
                     /locus_tag="IWJ07_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAP9333207.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            5167..5601
                     /locus_tag="IWJ07_001625"
     CDS             5167..5601
                     /locus_tag="IWJ07_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAP9333208.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5767..7812
                     /gene="parE"
                     /locus_tag="IWJ07_001626"
     CDS             5767..7812
                     /gene="parE"
                     /locus_tag="IWJ07_001626"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAP9333209.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7824..10274
                     /gene="parC"
                     /locus_tag="IWJ07_001627"
     CDS             7824..10274
                     /gene="parC"
                     /locus_tag="IWJ07_001627"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAP9333210.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            10551..12782
                     /gene="pflB"
                     /locus_tag="IWJ07_001628"
     CDS             10551..12782
                     /gene="pflB"
                     /locus_tag="IWJ07_001628"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAP9333211.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            13026..13787
                     /gene="pflA"
                     /locus_tag="IWJ07_001629"
     CDS             13026..13787
                     /gene="pflA"
                     /locus_tag="IWJ07_001629"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAP9333212.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            13872..14798
                     /locus_tag="IWJ07_001630"
     CDS             13872..14798
                     /locus_tag="IWJ07_001630"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAP9333213.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            14980..15873
                     /locus_tag="IWJ07_001631"
     CDS             14980..15873
                     /locus_tag="IWJ07_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAP9333214.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            16007..16132
                     /locus_tag="IWJ07_001632"
     CDS             16007..16132
                     /locus_tag="IWJ07_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9333215.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(16266..16898)
                     /locus_tag="IWJ07_001633"
     CDS             complement(16266..16898)
                     /locus_tag="IWJ07_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333216.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(17046..17588)
                     /locus_tag="IWJ07_001634"
     CDS             complement(17046..17588)
                     /locus_tag="IWJ07_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAP9333217.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            17723..18112
                     /locus_tag="IWJ07_001635"
     CDS             17723..18112
                     /locus_tag="IWJ07_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333218.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            18116..18880
                     /locus_tag="IWJ07_001636"
     CDS             18116..18880
                     /locus_tag="IWJ07_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9333219.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            19009..19956
                     /locus_tag="IWJ07_001637"
     CDS             19009..19956
                     /locus_tag="IWJ07_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9333220.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            20086..20826
                     /locus_tag="IWJ07_001638"
     CDS             20086..20826
                     /locus_tag="IWJ07_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333221.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         7381 a         3573 c         4409 g         5636 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaaatgata ttttacgagg aggaaacaaa
      541 catggtcgaa tcaccatttc attcaaccac tatctgtgcc gtcgaaaaag acgggaaatt
      601 tgctatggct ggagatggcc aagtaacaat gggtgaatct gttgtcatga aaggcactgc
      661 aaagaaagta cgacgtatct acaatggaga agttgttgta ggtttcgccg gaagtgttgc
      721 agatgcgttt actttagaag aaaaatttga aggcaaacta aacgaatata acgggaatct
      781 acaacgcgca gctgtcgaat tagctcaaga atggcggaca cagcaatcta tgcaaaaact
      841 tgaagcgatg ctgatcgtaa tgaatgataa agaaatgctt cttgtttcag gtacaggaga
      901 agttatcaca ccagatgatg gtatcttagc tatcggatca ggagggaatt ttgcgttggc
      961 tgcagcccga gcaatgaaaa actatgctca aaacgacatg agtgcgaaag aaattgcaga
     1021 aaatgcgtta aatattgccg cagatatctg tatctttact aatcataata ttatcgtaga
     1081 agaactgtaa gaggtgagta gatatgtatg aattaaaaca aacaccaaga caaatcgtaa
     1141 gtgaattaga tgaatatatc gtaggacagc acgcagcaaa aaaatctgtt gccgttgctt
     1201 tgcgtaaccg ctatcgccgt ttgcagttag atgaaaaaat gcaacaagat attacaccaa
     1261 aaaatatgct gatgatcgga cctacagggg tcggtaaaac agaaatcgca cgacgtttgg
     1321 caaaaatcgt caatgcacca tttgtcaaag tcgaagcaac aaaatttact gaagtcggtt
     1381 atgtcggaag agatgtagaa tcaatggttc gggatttagt agaaaatgcg atccaaatcg
     1441 tagaaaaaga gcagtacagc cgtgtataca caagtgctaa aaaaaatgca gaaaaacgtt
     1501 tggtaaaaat cttggtacct ggtatcaaaa aagaacagaa acaatccact aatcaatttg
     1561 aacaaatgat gaatcttttt aatgctagtc agcaaaatac cgctgaaccg caagaagaag
     1621 tgacagaaga tattcgaatc aatcgccgta cagtcttgga acaactacaa aagggtttac
     1681 tggatagtcg tgaagtaacc attgaagttg aagaccaaaa gaaacctgca ccaatgatga
     1741 acaatggatt ggagcaaatg gggatcgacc taaatgaaac tctaggtgct ttgacaccga
     1801 agaaaaaagt ggaacggacg gtaacagtca aagaagcttt agagttattg atcaaggaag
     1861 aatcagcaaa attagtcaag gatgctgata tccatagtga ggcaatcaaa ttagcggaag
     1921 aaagtggaat tatttttatc gatgaaatcg ataagatcac ttctaaatca cagcaatctg
     1981 gagaagtatc cagagaaggc gtccaaagag acatattgcc aattgtcgaa ggttcacaag
     2041 taaacacaaa atacgggatc atccaaacag atcacgtcct attcattgct tcaggagctt
     2101 tccatttgtc taaaccaagt gatctaattc cagaacttca aggacgcttc ccgatacggg
     2161 tagaattaga tgatttgaca gctgaagatt ttgtgaagat tctgaccgaa ccaaataatg
     2221 ctttagtaaa acaatacgtc gcgttgatcg gtacagaaaa tgtgtcagtt acgtttacaa
     2281 aagaagcaat tgaacggatc gctcaaatcg ctttcgatgt caaccgtgac acagataata
     2341 tcggcgcacg cagactgcat accattttgg aaaaactact agaagatcta ttatttgaag
     2401 caccagatat gcaaatggga gaaataacga tcacagaagc atatgtggat gacaaacttg
     2461 gctcaattgc ggcaaatgaa gatttaagtc gctatatttt ataaaaaatc ggaggacgac
     2521 aatgacagac ttgctaacga aaacccgcaa gataaacaca cttttgcagc aaaagaatac
     2581 ttttgacatg caagcagatt tgccttacga taaaatggct gtcacactag gtggtattct
     2641 tgacagcaac acctatatca taggaagtga cggagtatta ctaggttata atgaacgtca
     2701 cgatgtgaac aatgaacgaa tcaaaacaat gtttgttgag aaacaatttc ctgaatctta
     2761 tactgataca gtggcacagt tgatgaaaac tgaagctaat atcccaatta ctagtgatat
     2821 tactgcattt ccggtagagt caagagagaa atatccattt ggtctgacga ctgtcgttcc
     2881 gatctttgga gctggcgaac gtttgggtac gattatctta tctcgtatgg aacaatcgtt
     2941 tgcagatgac gatctagtct tagcagaata tggagctacg gttgtcggta tgcagatcct
     3001 ttatcagaag tctcgaagca tcgaagcaga tgtacggagt gcaacggctg tccaaatggc
     3061 aatcaacaca ttatcttata gcgaattgaa ggctgtacaa gctatctttg aagcattgga
     3121 tggagatgaa ggtcgtctga ctgcttctaa tattgccgat agtatcggca tcactcgttc
     3181 tgtgatcgtc aatgctttac gaaaattgga atcagctgga atcattgaat ctcgttcgtt
     3241 aggcatgaag ggaacttatt taaaagtatt gaattcccgt tttaaagacg agttagcaaa
     3301 acacagttat tagaagtaag gggactgggg cagatagaaa tgtttgttcc agccttccca
     3361 ttttaaaaag taagtcgaag ttggaggaat ggcaatgact gttattattc aaaatgacca
     3421 gctgatcgcc gaaatcgctg aacacggtgc ggaattgatc agtctgaaat caaaagaaca
     3481 tggtttagag tatatctggc aaggtgatcc agcgtattgg ggcagacatg caccggtttt
     3541 attccctttt gttggacgtt taaaagatga tcagtatgtt tatcaaggga aaacatatca
     3601 aatggggcaa cacggatttg ctcgggatat ggattttgaa gtcattgaac atggacaaga
     3661 aatggctagt ttcatcttaa ggagtacgcc ggagacatta gaaaagtatc catttgattt
     3721 tgaattagtg atcagctatg aattaggagg agacggcatc acggttcatt atcaagtggc
     3781 aaataccggt gaaaaagaaa tgttcttttc tatcggtgga catcctgctt tcaatgtacc
     3841 tcttgaagaa ggattgtcat ttgaagattt ctatctggca ttttctccat tgaaatcaag
     3901 attacgccta ccattgaaag gcccattcat cgatatggat caaaagacgt taggtcagac
     3961 aaatacgaat ctggctttga atcgtgaact ttttgagcaa gatgccttgg tttttgaaac
     4021 aaggggtcta aatgcgtacg ccattcgaag tgaaaaatct tcccatagcg taactttaag
     4081 ctataaagat atgccttatg tagggatctg gtctccttat ccaaaagaag cgcctttcgt
     4141 ttgtatcgaa ccgtggtgcg gaatcgctga cacggtgaac agtagtggaa acttgatgga
     4201 aaaagtaggg atcaatcaac taggtgccca tgaaatcttc aagacaaaat attccatcac
     4261 agtgaagtaa gaggttgtga ccgagaagtt agcagggaac accgtttacc aaggtgtgta
     4321 aaaaagctat cctgtaagta ataagggagt gtgacatcga tttgtcacac tcccgtttta
     4381 tctctttgga atgaaaaagt atcaggattt tttcttgctg ctattcaaac caaacgggat
     4441 tcgactttca gtatggtttc tgatacgctt gatattgtct ttatgccgat aaaagataaa
     4501 ggaagaaact aaaaaggcaa tgatcgtcag taaccaattg ggcttagcca aaatcacagg
     4561 agctacataa ggaaggacga tcgtagataa agtgatcaat actgcactga tcatactgct
     4621 caaactcacc atacttgtac aaaataaact aacgataaag atgatacaag aataaacaaa
     4681 aaatacagga ttaaatccta aaagcattcc tgcacttgtc gcaacagctt ttcctccttt
     4741 gaacccagca aaaatcggaa atgtatggcc aagaatggcg cataccccaa accaaagagg
     4801 gttaatccca gaaaggtgga agatcatcgg caaacaagta gccaatgtac cttttaaaat
     4861 atccataagc aggacaacaa tcccggctgt tttgcctaat acacgaaatg tattggtcgt
     4921 tccagtatta ccgctcccgt gttgtcggat atcttttttg aaaaacaatt tgccaatcca
     4981 gacaccggaa ggaatagaac ctaaaagata tgctaaaata agtaataaga tcaatttcat
     5041 tttttaccct ctttcaagtt gaaaactatc ctttatttta gcatgaatag gtaacaggaa
     5101 caattgaaaa aatgaaagat acaagtataa tagatggaat aaaaagaaaa gcaaggggtg
     5161 gcagaaatgt ctgtaaaaaa tccaacacaa gaaacgattt tcaactattt ggaagaagca
     5221 aaaaatattg ccgtcgtagg cttgagcaat aaaccagaac gaacaagtta catggttgca
     5281 caggtattgc aagacaatgg atataaaatc ataccagtaa atccattatt ggctggtgaa
     5341 gaaattctag gcgagaaagt ctatgcgaat ttgacagaaa tcgatgatcc aatcgatatc
     5401 gtagatgtat ttagacggag tgaattttta cctgaaatag caggtgattt tttgaaaact
     5461 gatgctaaag tgttctgggc acaattaggg attgaaaaca aagaagcagc tgagatgcta
     5521 agaaaagcag gacgtacaga tatcgtaatg gatcgttgta ttaagattga acttggtaag
     5581 ttgaaagaaa accaaaaata aaaatgacca caaagaagag atgatcgata gaggggaatg
     5641 tcgaaaaagc ggttaggaga taattaaaat tgatttctcg tctaccctct tttttttcac
     5701 gaccttttct agtatgataa tcgtggtgcg gttttgccgc aaagtattat tgaaggagcg
     5761 tttgccttgg caaaaaaagt aaacaacgaa tacaatgact cgtccatcca agtattggaa
     5821 ggattagagg cagttagaaa aagaccaggg atgtacattg gatccactga cagccgaggc
     5881 ttgcatcatt tagtctatga aatcgtcgat aatgcagttg acgaagcatt atcaggttat
     5941 ggcagtgaga ttgatgtgac catccatgaa gacaatagta taactgttgc agatagcgga
     6001 cgtgggatgc ctgtaggaat gcatgcttcc ggaataccaa cagttgaagt gatttttact
     6061 gtgctgcatg ctggcggaaa gtttggtcaa ggagggtaca aaacttctgg tggactgcac
     6121 ggtgtaggag ccagtgtggt aaatgcctta tcgaaatggc taattgtaac gatcgttaga
     6181 gatggcgtag agtatcagca gaaatttaaa aacggcggta aacctgacgg tactttgaaa
     6241 aagataggaa aaacaaaaaa agcaaacggg acgactgtcc attttttacc agatgataca
     6301 atattcagta caacgaaatt ttcatatgaa attttagccg aacggttaag agaatctgcc
     6361 tttcttctaa aaggcgttaa aatctctctt tctgatcttc gtggagaaga accagtcaag
     6421 gaaatttttc attacgaaga aggaatcaaa gaatttgttg attatctgaa tgaagaaaaa
     6481 gatacattga caccagttgt ttatttttca ggagaaaaag aaggtatcga agtagaggtc
     6541 gcttatcaat acaatgacgg ttattcagaa aatgttttat cttttgtcaa taacgttcgg
     6601 acaaaagatg ggggaaccca tgaagctggt atgaaagcgg cgatgacgaa atcttataat
     6661 gaatatgcga gaaaagttgg tttgctgaaa gaacgggaca agaatctgga aggtagtgat
     6721 ttcagagaag ggttagctgc tgtactttct atccgtgtac ctgaaaatct tctgcaattt
     6781 gaagggcaaa caaaagaaaa acttggtacg ccagttgctc gtaccgtagt agataacgtc
     6841 atcagtgaac aaatgggctt ttatttgcag gaaaatagcg aaatgagcca gatgttggtc
     6901 cgtaaagcaa tcaaagccag agaagcgaga gaagcagcac gtaaagccag agaagaaagc
     6961 cgaaatggga agaagcgtaa aaaaggcgaa tctcttcttt caggaaaatt gacacccgca
     7021 caatcccgaa accctaagaa aaatgaattg tatttagtcg aaggggattc tgctggcgga
     7081 tctgctaaac aaggaagaga cagaaagttc caagcaattc tacctttacg aggaaaagtc
     7141 atcaataccg aaaaagctaa aatgcaagat atcttgaaaa atgaagaaat caatacgatg
     7201 atctatacga ttggtgcagg agtcggtcct gagttttcta tcgaagactg taattacgac
     7261 aaagtaatca tcatgaccga tgcggataca gacggcgccc atattcaagt attactgctt
     7321 actttctttt atcgttatat gaaaccgctg attgaagctg ggaaagtata tatcgcgtta
     7381 ccgcctttat ataaagtatc caaaggacaa ggaaagaagc aagtcattga atatgcgtgg
     7441 acagatgacg aattggcagc aatgattaaa aaagtcggca aaggatatat gctgcagcga
     7501 tataaaggac ttggagagat gaatgcggaa caattgtggg aaacaacgat ggatccgact
     7561 tcccgtacgt tgatccgcgt ccgtatcgat gatgctgcgc aagctgaacg tcgagtaacc
     7621 acactgatgg gtgacaaagt agaacctcga agaaaatgga tcgaaaatca cgtgcaattt
     7681 acgctcgaag aagatggcag tatcttagat aaaaaagagg atactgaaat ctctccttca
     7741 gtttctaatg atttattaga tgaagaacga gcagataaaa atgaaaacaa tcagttattt
     7801 gaagtagaat aggaggtcac ctgatggaaa atcgacaaga agtacaagaa ttaactttag
     7861 aagaagtgat gggtgaccgc ttcggacgtt attccaaata tattatccaa gaacgtgcac
     7921 tgcctgatat ccgtgatgga ttgaagccag tgcaacgccg gatattattt gccatgaaca
     7981 aagatggcaa tacgtatgac aaaggattcc gtaaatcagc aaaatctgtc ggaaatatca
     8041 tggggaatta tcatcctcat ggtgacatca gtatttatga agcgatggtc cgcatgagcc
     8101 aagactggaa gttgcgggaa gtactgatcg aaatgcacgg gaataacggg agtatggacg
     8161 gtgatccacc tgctgcgatg cgttatactg aagccagatt atccaagtta agtggagaga
     8221 tgctagcaga tatcgagaaa gaaacagtcg atctcgtgtg gaattttgat gatacagaaa
     8281 aagaaccaac agtccttcca gctcgatacc caaaccttct cgtcaatgga tcgacaggta
     8341 tatctgctgg atatgcaaca gaaatcccga ctcataactt agctgaagtg atcgatggca
     8401 caatctatat gatcgatcat ccacaagcga gccttgaaaa attgatggaa tatattcctg
     8461 gacctgactt tccaacagga gggatcttgc aaggaaaaga tgaaatcaaa aaagcttatg
     8521 aaacaggacg aggaaaagtt attcttcgtt cgaaaacaaa gatagaggca atgaagggta
     8581 ataaacagca aatcgtgatc acagaaatcc catatgaagt aaataaagcg accttggtga
     8641 aaaaaatgga tgagatccgt ttgaataaga aaatcgatgg aatagctgaa gtacgagatg
     8701 aaagtgatag aacaggtctg caaattgtcg tcgaattgaa aaaagacgcc aatgctcaag
     8761 gaattttgaa ttatttgttc aaaaacactg agctacagat caattacaat ttcaatatgg
     8821 tcgctatcga ccatatgact ccgcatcaag ttggactgaa agacatttta agaagttata
     8881 tcgagcatcg aaagcaagtc ataacaaaac gtagtcaatt cgatttagcc aaagctcaaa
     8941 aaagacagca tatcgtagaa ggattgatga aagctttatc gattctggat gaagtaatcg
     9001 caacgattcg tgaaagtaaa gacaaaaaag acgccaaaaa gaatttagtc gacgtctttc
     9061 aatttacaga agaacaagca gaagcaattg tcacattgca attataccgt ttgacgaaca
     9121 cggatatcac agagcttcaa aaggaatccg aaagcttgat tgcgcaaatc acagagttga
     9181 ataaaatcct ttcaaatgac aaagagcttt tctctgttat gaaaaaagaa ttgcgagaag
     9241 tgaaaaaaaa ttattcttcc gctcgtctaa cgacaatcga agacgaaatc gaagaaatca
     9301 aaattgatac gcaagtctta gtggcacaag aagacgtaat tgtcagtgtg acgcgagaag
     9361 gctatgtgaa gcgaacaagc cttcgttctt atagtgcttc caaaccagaa gaaatcggga
     9421 tgcgagaagg cgactacttg ctatactcag gtgaattgag tacattggac catgttcttc
     9481 tgatcacaaa taaagcaaat gtgatctacc gccctgtcca tgaactaccc gatttgaaat
     9541 ggaaagatgc tggtgagcac atttctcaaa caatctccaa tctgtctgta gacgaatcga
     9601 ttttagctgt tttcccatat caaaaaattc aagcagagaa aacatttgta ttcatcagca
     9661 agaatggatt gatcaagcaa acacgaatga cagagttcga accatggcgc acctataaga
     9721 gccgtccgct cagctgtatg aagctaaagg cggaagaaga tgaactgatt gctgtgtatc
     9781 tgaaagaaga tcagccaggc ttagatgttt tcttagtaac gcatcaagga atgggtttgc
     9841 gctatccttt aacagatgtt cccgttgttg gagcaaaggc agctggagtg aagtctatga
     9901 atttgaaaga tggagatagg attatcaacg ggctgcttgt gttagcagaa ggtgatacgc
     9961 cgatcgtaat cgttactcag cgaggagctg ttaaacgcat gcttgctcaa gaaatcagcc
    10021 aaacttcacg tgcgaagcgg ggagtgacgg tcatgcgtga gctgaaaaaa aatccgcacc
    10081 gtattatcta tatgtcggaa ggaagatcaa aagaaataac cttgatgaat caaaaagggc
    10141 agcagttaac gattgaccca actgatttcc ctataggtga tcgaacatcg aatggatcat
    10201 ttgcgatgga tgagaaaaaa ggtggagaag tcatcgaagt tatcgatgct cctgagattt
    10261 tcatcgagtc gtaactgttg tagtatttta atgaagagac gccatctgta ttaatacagg
    10321 tggcgtctct ttttcattca tttttaataa atccttatac gtttgcaaaa aaagatgaaa
    10381 gtgttcacaa tgcttaaatg ttggtatgtc aacgttgatt taaatatatg taaataaaac
    10441 ataaaaattg ttaaataaaa aacaagaaaa cccttgcaaa gatagggtgc tgtgttatac
    10501 taatcacgta aaaactgtta cataaaacag aaatttagga ggtccataca atggagcaat
    10561 ggaatggatt taaaggtacc aaatggaaag aaggcgtaga cactagagac tttattcaaa
    10621 ataactacac tgaatatcgc ggtgatgaca gctttttgga aggcccaaca gaagcaacag
    10681 aaaaattatg ggatgaattg caaaagttaa atgatatcca attcgagcgt aacggtgtct
    10741 atgatatgga cacagaaatc gtatcaacta ttacttcaca cgatgcaggc tatttggata
    10801 aagatttgga aaaaatcgtc ggtctacaga cagacaaacc tttgaaacaa gcattcatgc
    10861 cgttcggcgg aatcaatatg gctaacaatg cgctaaaaag caatggctat acaccaaacg
    10921 atgaattgac acatattttc actgactggc gcaaaacaca taatcaaggt gtatttgacg
    10981 cttatactgc tgaaatgcgt gctgctcgta aaaacaaaat cattacaggg cttccagacg
    11041 cttacggtcg cggacgtatc atcggtgact atcgtcgtgt ggcattgtat ggaatcgact
    11101 atttgatgga acaaaagaaa aaagatcatg atatgactgg ttatcataca atgagtgaag
    11161 aagtgattcg tttacgtgaa gaaatcactg aacaatatcg tgcattagcg caaatgaaag
    11221 aaatggctgc gaaatatggc tatgatattt ctaaaccagc tgcaaatgca aaagaagctg
    11281 tccaatggct atacttcgga tatttagcag caatcaaatc acaaaatggt gctgcgatgt
    11341 caatcggacg tatctcagca ttccttgata tctatatcca acgtgatttg gataatggag
    11401 taatcacaga aaaagaagca caagaattga tcgaccactt agtaatgaaa ttacgtatgg
    11461 tcaaattcgc gcgtacacct gaatataacc aattattctc aggattccct atctgggcta
    11521 ctttatccat tgcaggtatg ggattagatg gtcgttcatt agttacaaag aatgatttcc
    11581 gtctgcttca tacattaaca aatatgggac cttcaccaga accaaacttg actgttttgt
    11641 attctgaaca ccttccagaa ggattcagaa catatgctag cagaatcgct atcgaaagtt
    11701 cttcgattca attcgaaaat gataatcagc ttcgtgaaca atggggttct gatgattgtg
    11761 cgattgcttg ctgtgtatca ggaacagttg tcggaaaaga catgcaattc tttggcgctc
    11821 gtgctaactt ggctaaaaca ctattatatg ccatcaatgg cggagtcgat gaagtgactg
    11881 gcgcccaagt tggaccagaa tatcgtccaa tcacatcaga aatactagac tttgaagaag
    11941 tgaaacataa cttcatgaat atgatggatt ggttagcaga actatatgtc aatacgttga
    12001 acatcattca ttatatgcat gataaatatt cttatgaagc agctcagctg gctttgatgg
    12061 attctgactt gcaacgcaca tttgctacag gtattgctgg gatctctcat gcggcagaca
    12121 gcttgtcagc gatcaaatat gcaaaagtaa aagcaatccg taatgaaaaa ggaatagcag
    12181 ttgattttga agtggatggc gacttcccta aatacggaaa tgatgatgac cgtgcagatg
    12241 aattagtttc atggatcttg gaatacttca tgggacaaat caaacgtcat aaaacttacc
    12301 gcaactcaaa accaacaact tcattattga cgattacttc aaatgttgtt tacggtaaaa
    12361 atacaggaaa tactcctgat ggccgtgaag ctggaaaacc acttgcgcca ggagcaaatc
    12421 caagttacaa tgcggaacaa agtgggttat tagctagcct gaactcaaca gctaaaatga
    12481 attacggctg tgcattggat ggaatttcaa atacccaaac aatcaatcct tcaggtcttg
    12541 gaaaagaaca agatacaaga atcgaaaact tgcgtaatgt cttagatgga tacttctcaa
    12601 aaggagctta tcatttgaat gtcaatgtat tcagtaatga tttattgcgt gatgcaatgg
    12661 aaaatccaat gaaatatccg aacttgacga tccgggtatc tggttatgca gttaaattcc
    12721 gtgacttgac aagagaacaa caattagatg tcttgatgag aacttcacac gacagaatgt
    12781 aaaaagttcg tgaaaaaagt ttttcgactt agcgtcgaaa ggacgttgaa tagccatcgc
    12841 atcaaaatcg aataatcagt atgcttattt gaaagaaacg atttacaggg gcaggacttg
    12901 tatgttccgt ccctgtaaat tttacacata acgaagcggc aattgcgaaa aattttgttc
    12961 agaaagcttc gcttcgacag ctaacttgta cttaaaatga aatcaaagaa aagatgtgaa
    13021 cttaaatgga agaaaaaaca atcggctatg ttcattctat cgaaactttc ggttcagtag
    13081 acggacctgg actgcgcttt gtcgtgttta tgcaaggatg ccgcatgcgt tgccaatttt
    13141 gtcacaatcc agatacttgg aatattggcg gagggaaaga atacacagca gatgaacttt
    13201 tagataaagc agaacgcttc agaccttact ggggagataa aggtggtatt actgttagtg
    13261 gtggagaacc tctattgcag atcgattttt tgatcgaact atttaaaaaa gcaaaagagc
    13321 ggaagatgca tactacattg gatacatgcg gaaaaccttt tacgtatgaa gaacctttct
    13381 tttcacgttt tcaagaattg atgaagtata cagatttact gttatttgac atcaaacaca
    13441 tcgataatga agagcataaa aaactaacgc attgggacaa tgaaaatatt ttagaaatgg
    13501 ctcaatattt gtcaaaaatc aacaaaccgg tttggattcg ccatgtacta gttcctgagc
    13561 gaagtgatta tgatgaatat ttgatccgac tagacaactt tattcaaacc ttgtcaaatg
    13621 tagacagagt agagattctt ccttatcata cgatgggaaa atacaagtgg gaaacacttg
    13681 gtttgaaata tccattagaa gggatcgaac cacctacaaa agaacgtgtc gaaaatgcta
    13741 aacgattgct tcatacagaa gagtatacaa aatatttaac gcgataggtc gcatcggaaa
    13801 gaagataagt attgcttctt tccgtttttt tgctattatt aaataggaga aatcacaaag
    13861 gagcgaatga catgtcaaaa attttagttt ttggtcacca aaatccagat acagatgcaa
    13921 tcggggcagc cattgcgttt tcacacttgc aaaatgaatt aggtaaagaa acagaagcag
    13981 ttgctctagg tacacctaat gaagaaacac aatacgcatt agaccacttt cagctatctg
    14041 caccacgagt agtagagtca gctaaagcag aaaatgcaga tgtcatgctt gtggatcata
    14101 atgaattcca acaaagtatt tcagatatca gcgaagtaaa tattttagct gtagtagacc
    14161 accatcgtat tgcaaacttt gaaacagcta atccattgta ctatcgtgca gaaccagttg
    14221 gatgtacaag tacgattatt ttaaaactat ataaagaaaa ccaagtaaca attcctaaag
    14281 aaatcgcagg aatcatgctc tcagctatca tttcagatac attgttgttc aaatcaccaa
    14341 catgtacgcc tgaagatgaa gcagctgcaa aagaacttgc agcaatcgct gaagtcgatc
    14401 taaacactta tggacttgaa atgttgaaag caggaacaaa tctaagtgat aaatcagctg
    14461 cggttcttct tgatttggat gcaaaaagtt tcccaatggg cagttccaac gttcgtatcg
    14521 ctcaagtcaa tacagtggac cttaatgaag tgatggatcg acaagcagag ctagaagcag
    14581 caatggaaga tgaaatgaaa gcaaatggct acgatctgtt cgtattagtt gtcacaaaca
    14641 tcttagacag tgattcagaa ttacttgttg ctggtaaagg aaaagaaaaa gtagaagaag
    14701 ctttccaaac aactttgacc aacaaccgtg ctttgcttaa aggtgtcgtt tcacgtaaaa
    14761 aacaagtcgt acctcaattg acggctgtat ttagctaatc aaatggtagt atgaagcatt
    14821 caaaatgaat ggataagcga ttgatataaa aagaaacata gaacatacta aaggccgaag
    14881 aaatggagga gaataacagt caattatgac tcttgttctt cctccttttt acgtaaaagc
    14941 cttttaagta aaataaaacg gatagaaaag agaacatata tgaactatta ttcaaaaaat
    15001 aaggaaaact tttatcaaaa actgaccgat gatccgcttt tttcttcatt gaccgattat
    15061 ctatatgaac atcgagaaca agaaacgatt ctaagagaat tgaaaaaaga attttcgcaa
    15121 aataaatttt cccattttct tgatttattg atcgatgcag gattaatcaa gagggaagaa
    15181 cgaagatatc atttgaactt tccaatattt gatcctaaag attacttgca acaagccact
    15241 tcggctgctg aaaccatagc tgaacaattg aagagactct ctgttgatga acaaaaatta
    15301 gcgatgggag aagttatttg ggcgtattgt ttcgaagatg aacggaaaga agcgtatttt
    15361 tatggagtaa gaaacagccg agagacagaa ttgctgagga caactgcagg aaatgagaag
    15421 taccgtttta tcacactttc ctctatagaa cacttcccgc ttactttggc taattacttt
    15481 tttgtacaga agaatcagct tcctgtaacg aaggcattca aagaattagc cgaacttatt
    15541 ggagacgtaa atgaatcgta cttttttgat caaatagaag ttatcgttga caggatacgt
    15601 aagaataaat ataaaaatcg ccggcctagt attttccatc agtctctact tgtgacgaat
    15661 actataaagg aagaagaatc attcacatta gaattaccta ttgtcgaaaa aaacaatttt
    15721 gagattgaat taccaacatt ggatccttca ttgacaatgg aggaaatggc ctttcttaaa
    15781 cgacaaatct tttctgagct gagcaagaaa tttataccgc atgcattttc ttatatcaaa
    15841 gaatatggaa cagttttagt gagtaaaacc taagagagca gtaagcaaaa attatactga
    15901 ctcttgcagg aggagccttt ctcttccttt atggttaaaa gaattatttg agtattttgc
    15961 ctatctacca cctattagat tcactacatg gtctggaaac aaaggaatgc aaaaaaatgg
    16021 tgagaaatgt ggaatgacaa aggaagtagt gataagaaaa gttagatttt taaataatca
    16081 atattatgat tcagtcaagt atggcatttt atgggaagaa ctagctgatt agattaggaa
    16141 gtccatttga taatccagaa tcactaagaa acgaatggcg agtcattaat aatttatcca
    16201 atcttgggtt attcttttag attgaagtgt ttaaaactaa aaaagcattt atgaagtcgt
    16261 aatagtcagg cttcaaaatg ctttttattt tgtttgtttt ttctttgcca cataaataca
    16321 cctagactga cgatgaagag catatccaac agatttcctg cgacttggat cgtcgaaggg
    16381 ttggtcagtg gcattaaagc aaggacaaaa aaggaaaaca agctgtaaat aaaacaggtg
    16441 acaggaacta atcgaaagaa gagcggacat ttcaattggc taaaaatgag tgaaaaaata
    16501 aaataagtaa aaatggataa aatcacgact aaaccattca tgataaagaa gaaaagcgta
    16561 ttgtgattaa acgaataaaa aagaattgct aaaaaagaaa acgttttaaa tgcaatcaat
    16621 acgcttgaag cttcgtttaa cgtaccagga tgtcgatgaa aaaaataggc accgaaataa
    16681 acagaaaaca gtaagccgca aagagaagac aaaatcaaaa aaaacggatt ttcttcggct
    16741 tctgtgatat cagaatgatc caaaagcaac cagcctaatg cactagaagg cgcatgtgag
    16801 gtcttaaggt atgaataaca tagaaaaaat aagatcgtca aagttgcaaa aaaagagcct
    16861 aaatgtaaaa aattagtggt tctacgttga atttccataa tatctccaaa ttaaattgat
    16921 aagtatataa agtataataa caaattgatg agtatatgtc gatgtttttt aatcaaaata
    16981 ataaataaaa actgtctaac atttaaaatt taatttagat attagacagt ttttaagttt
    17041 gtgagttatt ttccttgtcg ttttctcact tttttagtcc agaaaccacc tgaaatatat
    17101 tttggctcat aagaaatgat aaaagcacgc tcttcgattt ctttgatcag gcgatacagt
    17161 tcacgttcgc tttttctagg agaaagtatt tcgagaacta gccgctcacc ttccagcccc
    17221 attgcagaag tctgtgtcac accataaccg tgttctctca gtatacccgg caaactttta
    17281 tcttctgcgc tatttgctgg caaaatcgtt gtgaccatga tgtatcctaa tgccaatttg
    17341 tcttcaatca aaattccgac actgatacct actgcgtaac ctaacgcata gacaaataaa
    17401 ttcagtggat tatctaaacg atctaaaaca agggataagc cgacgatata aatcgtgatc
    17461 tcgaccatac tgacaagtgg agcaatcagt cgatagcctt tcattgtcaa catgaaacgt
    17521 aacgtgttca gagtgatata agcaaaatta atgaggaaga tcatccccaa catttttgga
    17581 tcaatcattt gtaaaactcc tttcatttat ataacaacaa tatcaaaaaa aatcgtaagt
    17641 gaagaaaaat tgaaacttta tacatacaaa aaaatgataa aatgattata atagaattat
    17701 aaagataggt taggtgatta aaatgaaaac ggtcaatcgt gtgttttgtg ccagccacgt
    17761 gctaagtctc cttctcagta tatacttagg tgtctattcg attcaacagc caattgaggt
    17821 agagaacatt gtttttcaga tgtcgctgac agatggttta attttaagcg tttttttctt
    17881 tttgatactg tttattggta atattttcgg tagcgttgcc agttttttga atttttctat
    17941 ttatccactc ctttcgctag cattaggagt ggtgggactt tgttctctgt gcttatttcc
    18001 cgagccattc agtcctgcat ttatattatt tggtacactg aacctgttcc agacaactgt
    18061 tggagcatgg ttgctatggc gatcaggaaa tttaatgaaa ataggtgagt aacaattgaa
    18121 tccaattata ttgaaacgtt ctgtccatca tattccagtg ttagaagttg ttctgaaaga
    18181 attaagagag gaacgtattc ccgttgtcat ctattatcac ggatggcaga caagtaagga
    18241 gttagttttg acgcaaggaa gaaaaattgc gcagcagggc attcgtgtga tcctgccaga
    18301 tgcgatgaat catggggagc gcaaacagcc ggtttcttct attccatcct atacattttg
    18361 ggatagtatc tacggaaatc tgtttgaatt tgacacactt atcaagcatt tagaaaaaag
    18421 aggtcttcta catgatcagc ttgcagttgg cggggtttcg atggggggaa tgacaacatg
    18481 cgccttgttg gctaaacatc ctcatatagt agcgggcgtg tgtttgatgg gttcccctgc
    18541 acctttggaa tatggaaaag aaatcgtaag gcgcgcatcg gaatataatt atgtcgtacc
    18601 aaaagactat tttgatttga tttcatgggt taagagctat gatttgtcat tgcagccgga
    18661 aaagttagct ggacgtcctc tgttcttttg gcatggacaa gctgacgaaa aaatccctta
    18721 tatacaaacg gaacgatttg tagaaaaaaa tcggcataag tcttacggaa aagaaattgt
    18781 ttttcgttcc tcaaaaacac agcgacatat ggtacaaata ccactaatgg aagaagcttc
    18841 cactttttta gcaaaaaatt tgataaaaaa gtataaataa ttgaaacgga aaatcactca
    18901 tgacgataga ggattttccg ttttttattt tgggctataa taaactctgt taggaaaatt
    18961 tggtataatt gatagtaaga atgacaacat gttagaaagg aagagagcat gcccacttta
    19021 gcagatgttg caaaacgtgc caatgtttca aaaatgacgg tctctcgagt catcaatcat
    19081 ccggaacagg tgactgacga attgaaagag ttagttttct cagcaatggc tgaactggat
    19141 taccgtccaa atattgctgc aaaagcactt gtcacgaatc ggtcgcagat catcaaatta
    19201 tttatactgg aagaaatcga cacaactgag ccctattaca tgaatttgct gatgggaatc
    19261 gctaaacgga tcggtaaagc gcattattct ctccaattag ttacaaatga tgccttcgac
    19321 gtcggttctt gtgatggata catcatcaca ggtgtcagaa aaaaagactt cgattggatc
    19381 gaccgtttgg aaaaaccagt tgtcttgttt ggggaaaatt ctgaaggata tgattatgta
    19441 gacagcgata atgaatatgg tacagcaaaa gcaacgcaat atgccttaga aagagattat
    19501 caaacgatta tttatatcgg tatcgaagtg gacgagcctt ttgaaatttc aagggaaact
    19561 ggttatttat ctgtgatgaa ggaagttgat cttcctagta aaatcttgcg gtttccaaat
    19621 cattcgacag aagtagaaag atatatgaca gaaaactggg cagattttac agataaaact
    19681 tgttttgtat gtagttcaga tcggctagca ctaggagcag taagagggat tataaatgct
    19741 ggaggacact tgcccgaaga atttggtgtg attggatttg atggggtatt tttagaccaa
    19801 atcagttcac ctaaattgac aacgatcaag caagatatta tacgtatggg tgaagtatgt
    19861 ggagaaatgt tgttaaaaaa aatagaagaa aaaggggcga gtcaaggtta tcgtcgtttt
    19921 tttcctgaac tagttatcag agagacaacg aattaagtct tattactata tatatattta
    19981 tgaaattcat cgctattttc ttaaaataat cagcgcattg ctactgaaaa caatgaatct
    20041 gtgctattat tgagaaaagt tccatttttg agaaggatgg tttgaatgaa agaaatcaga
    20101 cgaaaacatt tccgttttcc tgcgtacgat gatgagttag gcgtaaaatt aaccaacgac
    20161 aagaagcgag tattatttga cggacaagaa gatcttttga ctgaacagaa tcagtccccg
    20221 tttgaacaga tgaaagaatt tcatttcacg aacacgaaca ccgagaaaat ccgtcgcaag
    20281 cgtcgtgcaa caaatggatt gaagtcagct ccgcgtaagg aaaatttgga aaagcataaa
    20341 ggaaaattac cggattatac cggccattac caatcagaaa tcacatcgac aggacgaaaa
    20401 aaactttttg gtgattcctc tcgttctgtc agacaaagtg aaacaaggac agagtccaag
    20461 catactacac gaaacgcatc tgttgacacg aaggtgcggg agcgttctta ttttgttcct
    20521 aaatatgtac ccgcatcgat tatcccagac cctagagaaa gcaaaatttc tgaggatcat
    20581 ttaatcgaat cgatgaaaaa aacggatgac tcttatctgt tgttcgatac agaaccgacg
    20641 ccgtatcaag aaaaaagaaa tggtgagccg tctgtacgaa cattcaatcg aacaaaaacg
    20701 ttagaggaaa caaagcagtc agaagaaaaa aaatcgaaaa aaagccatgg tatcttagaa
    20761 cgctcattac aaggattgat cgatgatcaa ggaaactctt tgggagaaaa tagctatttt
    20821 aaataagtga tatacaaatt tatattcggc tacagttgga aaggaaacgc tgcggcgttt
    20881 ccttatttta tactctatcc gaaagtttat atcgtatcct caaatttttt tgagtttaag
    20941 cgaatttttt ctcccaataa gtaagaaaat aatggcaaaa taaaaaaaca aactatttt
//
LOCUS       DACDPS010000041        20360 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.041, whole genome shotgun sequence.
ACCESSION   DACDPS010000041 DACDPS010000000
VERSION     DACDPS010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.041"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            290..1429
                     /locus_tag="IWJ07_001639"
     CDS             290..1429
                     /locus_tag="IWJ07_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAP9333222.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1496..2071)
                     /locus_tag="IWJ07_001640"
     CDS             complement(1496..2071)
                     /locus_tag="IWJ07_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9333223.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2076..3050)
                     /locus_tag="IWJ07_001641"
     CDS             complement(2076..3050)
                     /locus_tag="IWJ07_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAP9333224.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3074..3118)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3333..4301
                     /locus_tag="IWJ07_001642"
     CDS             3333..4301
                     /locus_tag="IWJ07_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9333225.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            4414..4692
                     /locus_tag="IWJ07_001643"
     CDS             4414..4692
                     /locus_tag="IWJ07_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP9333226.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4810..5046
                     /gene="secG"
                     /locus_tag="IWJ07_001644"
     CDS             4810..5046
                     /gene="secG"
                     /locus_tag="IWJ07_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAP9333227.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5158..5904
                     /locus_tag="IWJ07_001645"
     CDS             5158..5904
                     /locus_tag="IWJ07_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9333228.1"
                     /translation="MKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSLAEH
                     QLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5907..8264
                     /gene="rnr"
                     /locus_tag="IWJ07_001646"
     CDS             5907..8264
                     /gene="rnr"
                     /locus_tag="IWJ07_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAP9333229.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8286..8750
                     /gene="smpB"
                     /locus_tag="IWJ07_001647"
     CDS             8286..8750
                     /gene="smpB"
                     /locus_tag="IWJ07_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAP9333230.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9052..9771
                     /gene="atpB"
                     /locus_tag="IWJ07_001648"
     CDS             9052..9771
                     /gene="atpB"
                     /locus_tag="IWJ07_001648"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAP9333231.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9824..10039
                     /gene="atpE"
                     /locus_tag="IWJ07_001649"
     CDS             9824..10039
                     /gene="atpE"
                     /locus_tag="IWJ07_001649"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAP9333232.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            10130..10654
                     /gene="atpF"
                     /locus_tag="IWJ07_001650"
     CDS             10130..10654
                     /gene="atpF"
                     /locus_tag="IWJ07_001650"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAP9333233.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10641..11183
                     /locus_tag="IWJ07_001651"
     CDS             10641..11183
                     /locus_tag="IWJ07_001651"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAP9333234.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            11206..12762
                     /locus_tag="IWJ07_001652"
     CDS             11206..12762
                     /locus_tag="IWJ07_001652"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAP9333235.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            12776..13678
                     /locus_tag="IWJ07_001653"
     CDS             12776..13678
                     /locus_tag="IWJ07_001653"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAP9333236.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13701..15107
                     /gene="atpD"
                     /locus_tag="IWJ07_001654"
     CDS             13701..15107
                     /gene="atpD"
                     /locus_tag="IWJ07_001654"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAP9333237.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15121..15543
                     /locus_tag="IWJ07_001655"
     CDS             15121..15543
                     /locus_tag="IWJ07_001655"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAP9333238.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            16012..16242
                     /locus_tag="IWJ07_001656"
     CDS             16012..16242
                     /locus_tag="IWJ07_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAP9333239.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            16339..17640
                     /gene="murA"
                     /locus_tag="IWJ07_001657"
     CDS             16339..17640
                     /gene="murA"
                     /locus_tag="IWJ07_001657"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP9333240.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNCPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            17653..17829
                     /locus_tag="IWJ07_001658"
     CDS             17653..17829
                     /locus_tag="IWJ07_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP9333241.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            17964..18260
                     /gene="yidD"
                     /locus_tag="IWJ07_001659"
     CDS             17964..18260
                     /gene="yidD"
                     /locus_tag="IWJ07_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAP9333242.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            18324..18707
                     /locus_tag="IWJ07_001660"
     CDS             18324..18707
                     /locus_tag="IWJ07_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9333243.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHH"
     gene            18876..19760
                     /locus_tag="IWJ07_001661"
     CDS             18876..19760
                     /locus_tag="IWJ07_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9333244.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            19877..20110
                     /locus_tag="IWJ07_001662"
     CDS             19877..20110
                     /locus_tag="IWJ07_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAP9333245.1"
                     /translation="MSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            20198..20278
                     /locus_tag="IWJ07_001663"
     tRNA            20198..20278
                     /locus_tag="IWJ07_001663"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20232..20234,aa:Tyr,seq:gta)
     gene            20285..20356
                     /locus_tag="IWJ07_001664"
     tRNA            20285..20356
                     /locus_tag="IWJ07_001664"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20317..20319,aa:Gln,seq:ttg)
BASE COUNT         6762 a         3562 c         4423 g         5613 t
ORIGIN      
        1 tttttgcaac tagcttctgt atatgatgtt tacaaagaat ggtacaagaa aagatcatcc
       61 ttgaaaaagg aaagctaaca ataactagca tcatataaga gcttttaagg atagaacagt
      121 agaataggaa tctatgggca agtcctctcg tataaataaa ataggaggga agtagcagca
      181 tatttttcat aaagtggctc tctttcttca tttttacttt ggttgctcct atttgaaaat
      241 tcgagcacac tcaaatacaa taaaaagaaa tggcaaggga gaggattaga tgaaaaaaga
      301 agaaaaaatc gcgattttac aagagattat ccgaattaaa tcagtgaatg gaaacgaagg
      361 tgaagtagca gcctatttga ataagctttt ggcaaggcat gacataacag gagagattgt
      421 ttcttacaga gatggtagag ataatttgat tgctcgttac caaaagggcc aatcaggaaa
      481 agtgttaggc ctatctggac atatggatgt cgtggcggct ggtgatgaat cgtcttggac
      541 ctatgcaccc tttgcagcag aaatccatgg gaaccgtttg tacggacgtg gtgctacaga
      601 tatgaagagt ggcttggcag caatggtgat tgccatgatc gaactgaaag agtcaggaaa
      661 accatttaac ggaacagtca aattattagc cactgtcgga gaagaagtgg gtgaactagg
      721 tggggaacag ctcacgaaag caggctatgt agatgactta gatgccttga tcatcggaga
      781 gccaaccaat tacagtttga tgtatacgca catgggatca atcaactata cagtcacctc
      841 gcacggaaaa gaagcgcata gctctatgcc agaccaaggt tataatgcaa tcaatcacct
      901 aaatgaattc attacgaaag caaatgctga aatgaatcac ctcgctgaaa caatcgagaa
      961 tcctgtccta ggaaaaacaa tccataatgt tacattgatc agtggcggga atcaagtcaa
     1021 cagcatccca agccatgcgc agctccaagg gaacattcga tcgattccag aatacccaaa
     1081 tgataaaatc attgcattgc tgcaatcaat cgtcaatgaa ttgaatcaag agacagacta
     1141 tcatttggaa ttgatgatcg attacaacaa aatacctgtc aaagctgatc cagattctcc
     1201 tttgattcat tccattcagc aacaattcag ccagccatta cctttagttg gagcggcagc
     1261 aacaacggat gcagccgaat ttacgaaagc taaccattcc ttcgactttg tcgtctttgg
     1321 gcctggtgtc gttactttgc ctcatcaagt agatgagtat gttgaaatcg ataattattt
     1381 ggatatgatc gaaaaatatc aaggaattat tttatcctac ttagcgtaag atttttttaa
     1441 taaatgaaaa aacagagcca gattagcatt attttgctaa tccagctctg tttattcagc
     1501 ctatttttct ttttgaatat atcaagcgtt ggggcaacat agtgatgact aatgaaacaa
     1561 cgacgataga caacaagcga tcaaggtagt ctgtacccac ctgtaccaag atcacacttg
     1621 ttgtctgact taaacctaac ccatgtaata gctgaacgat catactggat cctgaggagg
     1681 tgatcccgcc aaataactga acggtaatga tcgaagaaac gatcgttcca ggtaccgaca
     1741 aaatcaaagc gagtaataac agtttcaagc tgtttttatc tttgccgaga tatcttggca
     1801 aaatcaatcc agcaagcaag cctgtgagaa tttggattgg cgaataatac agagaaaaaa
     1861 tatctgtagt cactccactt aaaataccgc tggacaatcc tgtcagcatt ccaggtacag
     1921 gcccaagcaa tgcaccagca aaaatcgttc cgatagaatc gagatagatt ggcaaacgaa
     1981 gccataaagc aatcgttcct ccaatataat tgatcccaat acacaaagcc atcactgtaa
     2041 gtacgcgagt tgccattttg tttttattca ttttgttagc ccatctttaa cctttccaaa
     2101 tctttcataa tcgtttcttc ttgtgcattt aaaagaacgg ttaaaaactt tcggaaaaaa
     2161 agacgagtat ctacttttgt cataatttta ctgttagcag gtttctgcca aaattcataa
     2221 cgatcaacca atgtttgtcc gcgacttatt ccttgagttt ctacagcagt gaagctttta
     2281 aagccttcac agatggactc atcgataaaa taagcaacag ctaatggatc attgatgaca
     2341 catcctaaga ttctttcttg tttccagtga aagtcaaaat aaaaacgagt gatcgctttt
     2401 agatactctc cctcttcagg attcatttgg cagcaatatt caaggatagt cggtgtcaag
     2461 acgatttctc ttgtcacatc taacccaacc atttctatcg gttgtttgag cttttcgaat
     2521 acataagaag ctgcttcagg atcacaccag taattgtatt cagcaactgg tgaacagtta
     2581 ccatggcttt tgtaagttcc gcccattgag acaaaacgat ccatatgttt cccaacagat
     2641 gggctgtttt tcaaagcaga agcgatattg gtcaaaggcc ccaaagcaat gactgatgta
     2701 tctgtttttt ctttgaaata atcagtcaga aaatcaactg catggagcga ttctgcctgt
     2761 ttggctaaga ccatcgggaa atttgtgtcc cctaatccat ccaacccatg agtgtcttgc
     2821 gcactaatga aaggttgctt caaaggtttt tccattcctt gataaacagg gatatctaaa
     2881 cgatcacagc gctccaaaca tttcagcaca ttctgcgtac cgatatctac agggacattc
     2941 ccgcaaacga cggtaatagc aactacttcg atatcagggg atttgacagc tagtgataaa
     3001 gctaaagtgt cgtcgatccc tggatcgcaa tcaataatta ctttacgcat tatttccaat
     3061 ctcctttctt tagagatact tagtttttta ttctgggaag tttccgaacc agtccaagac
     3121 gagtgtcttg tttataatgc acgaaattag tatactggct tttattgtga atgtaaatgc
     3181 tctcttacaa gtaaatctat tgagaagaac tttaagtaaa tgatacaatg aaaaagaaat
     3241 ggaatttacg tgtgccgaag aaaaagaatc ctattgatcg gtatattgta taataaatag
     3301 attatagttt ggcagaaagc ggcgggtgta cgatggttat tttttatttc attcttattc
     3361 tgatatttat aggaatcagt taccattttc tttcttattt ttatgcaaag aaattagtaa
     3421 gagcgggggt acagagaggc tatgactggt atgaagaaac tgggcaccgc ttgatggatc
     3481 ctgctgcagt cacgtcagta gaaaaaaagc gacaaaaatt agaagaagag tgtgaaagct
     3541 tctgggaaca aggaataccg aaaacgatca aaagttatga cgggttgaaa ctagctggac
     3601 agatgttcct ccaaccaact cagcagaata aatgggtcat ttgtgtacat gattatcgaa
     3661 gtacagggaa aagagatatg tctcatatag gtaaaagata tgcagagaaa ggattcaacg
     3721 ttcttatacc cgatttacgt gcgcatggag aaagtgaagg agaaatcatc ggaatgggat
     3781 ggctagaccg gctagattta attgcatgga tccaactcat cttagatgaa caaccagatg
     3841 catccattat tttgcacggc agttcgatgg gcgcttctac tattatgatg gcaagtggtg
     3901 aaaagcttcc atctgcagtc aaaggattta tcttagacag cggatatgtt tctgtttatg
     3961 cggaatttcg gtatatgtta agtaaaatca cggtctttcc taaaaaaatg atcatgagat
     4021 atgcgaatca ctatgctcag aaatacgcag gttattcttt aaagcaagca tcagctacaa
     4081 gacagcttgg tagtaaccat ttgcctttgt tagtcatcca tggtgaaaga gatcattttg
     4141 ttcctacaga agcagcatac acgattcaaa acgctacggc aggggataaa gcattactgc
     4201 ttgttccaga agcagaacat ttggaagcat cgatgaaaga tccgaaaacc tattggacag
     4261 tgattttttc atttattgaa caaagaataa cgatggattg attttctctt cctttttgta
     4321 agcaacacga ggataaataa ttgtttatag accatgagac tgttaaaata aggtaggaat
     4381 aaaaaaatat cagttatttg aggagtgcgg catatggaaa tcaaagagga aaaaaatcgt
     4441 cttgctttat tcaatgatga gcaacaagaa atcggagaaa tgacatggtc ggatgcgggg
     4501 ccagacatca tgatcattga ccatacattt gtcgatccag catatcgtgg tcaaaaatta
     4561 gctgaaaagc tagtgtatac tggagtagaa ctagcaagac gagaggggaa aaagattatc
     4621 ccttcatgcc cttacgcaaa aaaagagttt gagaagaaac cagagtatca tgacgtctta
     4681 cgccaatcat aataataaca gctgaaagat ttgcactttc agctgttttt ccatttatat
     4741 agagttgtca cggagtttta tctatgataa agttaaagag acgatatctt ttgggaagga
     4801 ggctgctata tgtataattt aattttggga atcgtgatcg ttttatcgat tgttatggtc
     4861 attgcgatta tgatgcagcc aagcaagcaa aatagtgcag cgagtgcatt taccggcggt
     4921 gcggatcaac tattcggcaa acaaaaagct cgcggctttg aagctgtcat gcagcgatca
     4981 actgcagtca tgggagctgt ctggatgatc ttattatttg tcctagcatt tttatcttcg
     5041 aagtagtcga ataagcctct cgcacaacgg cgggcggctt tttttattgc aaagagaacg
     5101 cttaagaaga agttatgaaa gaaactatgg taaaatgagg cgaaagaggt gaaacgagtg
     5161 aaacaattac caaaacccct ttatgcaaaa catggaaaac gagcagtttt gctgcttcat
     5221 gcgtactcag gaagtccaaa tgatgtccgg atgctcgcac ggtttttaga aaaagcagat
     5281 tatacagtct atgcgccgct atttaaagga catggaacga tggagccata tgatatcttg
     5341 caagaaaaag cagaaagctg gtgggcagat actaaaaaag ctatccattt tttacagtct
     5401 gaacagtttt ctgatattgc cgtattggga ttatcgatgg gtggcatctt tgctgtacgg
     5461 gcactagaag aagaatcagt aattggcggc ggttttttct gttcgcccct gtctcctgta
     5521 gaaacaaacg tgcctgaaaa ttttgaaaaa tatgtacgtc aagtgctgaa gatagcaggc
     5581 aaatcagaaa aagaaatcaa tgaaaaagct gtgtcttacc gctcgttagc tgaacatcaa
     5641 ttaatggata tacaagacca agcagcaatc gcagaaagta gactatcaga tatccaacag
     5701 ccaatatttt tagctcaagc tgggaaagat gaaatgatcg acccaaatgg tgtatttgaa
     5761 acggcacgca aattgagccg ccaacgagtg acacttcaat ggtatcctga aagcggtcat
     5821 gtgattacag ttggcacagc aagacgcgaa ttagaaaaag acgtgttgga atttttagaa
     5881 aaattacctt ggaatgagga ataaatatga caaaagaaac aataaaagag aaaattctct
     5941 ttttcatgga aagcagtcgc aaacgtagtt tttctatgga agaaatcgct gaaggtctag
     6001 gctttcaaaa aagcgatgat tttaaattac tagtacaaac gattgctcaa atggaaagag
     6061 aacagagtat cgttttcaat aaaaaaggaa aagtcaaact tcctttaaaa ccgatcttgg
     6121 tcgaagggac atttcgggca aatgaacgag gatttggatt tgtaacgatt gatcccgaag
     6181 aagatgatat ctatatcccg aaagaagcga ctggctttgc aatggatggg gataccgtag
     6241 caattgatat catgaagcga gcaaatacag atatggatcg cggagcagag ggaaaagtag
     6301 tggagattcg agaccgagcg actactcagt tagccggaga atttgttgct tattcagacg
     6361 aagagataca ggaaacggat ctttatggtg tggtcatccc gaaagataga aagcttaacc
     6421 aattcaaagt gtatgcagcg gcagagggag ttcgtccagt tgacggaagt attgtattga
     6481 tcgaactgac ccattaccct gaaaaagggt atgcaactag tttagaaggg ataatcaagc
     6541 aagtgattgg tcataagaat gaccctggca tggatatcct ctcgattgtc gtggctaacg
     6601 gtattcctac aaaatttcct gatgatgtgt tatctgaggc agatgaagtt ccagaccaga
     6661 tcagcgaaaa ggatattgtt ggacgcagag atttaagaga tcagctcatc gttacgattg
     6721 atggagaaga tgcaaaagat ctagatgatg cagtgacggt caaaaaatta gaaaatggta
     6781 attattttct tggagtacac attgcagatg tatcttacta tgtgacgcaa ggcagtcaac
     6841 tggacagaga agcatacgaa agaggaacaa gtgtctattt gaccgatcga gtgattccga
     6901 tgatcccaca acgtttatcc aatggtatct gttcattgaa tccccatgtt ccacgactca
     6961 ccatgagttg tgaaatggag atcgatccaa atggacaagt tgtctctcat gagatttttc
     7021 caagtgtgat ccaaacaaca gaacggatga cctacacagc tgtcaatcaa atcttagaag
     7081 atcaagatga agaaacgatg caacgttatg ctcagctcgt gcctatgttc caagaaatgg
     7141 gtgaattgca tcgaatcctt gaaatgatgc gaatcaaacg tggagcgatt tcttttgagg
     7201 atcgtgaagc gaagatcctt gttgatgctg aaggacaccc aaatgatatc cagctaagaa
     7261 gcagagggat aggtgaacgc ttgatcgaat cctttatgct tgctgccaac gaaacggtag
     7321 ctgaacatta taataaatta aatcttccgt ttatttatcg aatccatgaa cagccaaaag
     7381 aagaaaaaat gcagcgcttc tttgattttg cttcagcttt gggcattctg gtccgaggaa
     7441 cgaaaggaac gatcacacca aaagatttac agcatgtgat cgaaggagta gaagacaaac
     7501 cagaagctgc agttatcaat actatgttgc taagaagcat gcaacaagca cgctattcag
     7561 aagacaattt tgggcattat ggcttagcgg cagattacta cacacacttt acatctccta
     7621 ttcgacgcta tcccgatcta atcgtccatc gtctgatccg ttcttacagt gaagataaaa
     7681 gtgaaagcaa tcaaagttcg tgggctgcgg aacttcctga aattgcagat catagttcta
     7741 agatggaacg tcgtgcagta gaaacagaac gtgaagtaga ttccatgaag aaagcagaat
     7801 tcatgatgga caaagtggga gaagaattcg atgggatcat cagctctgtt gtgaaattcg
     7861 gcctgtttat cgaactgccg aatacagtag aaggattgat ccatatcaat gaattgaaac
     7921 aggactactt ccattttgtt gaaaatcatt tagctcttgt aggtgaaaga actggtctga
     7981 cttttaaaat cggacaaaaa gtgcgcgtaa aagtagtcaa agctgatcca gatgaacgag
     8041 cagtggattt tgaactcgtt tccgcagaag aggtcactgc gttggaacgt ccaaaagcac
     8101 gcaatggaaa atctggaaaa cgagaaggaa aacaagcagg caaacgctca gaaaaaaatg
     8161 gcaagaagaa caaacgatat agtgatagta agcaaacgtt cggtaagaaa aaagggaaaa
     8221 agccatttta caaaggtgtc aaaaagaaga aaaagaaaaa ataaaatgag ttggaggaaa
     8281 cagctatgcc aaaaggcgag ggaaaactaa ttgcacaaaa caagaaagct cgccatgatt
     8341 attcgatcat cgacacgatg gaagcaggga tggtcttgca aggaacggag atcaagtcga
     8401 tacgaaacag ccggatcaat ctgaaagatg gatttattcg cgtccgcaac ggggaagctt
     8461 tcttgcataa tgttcatatc agtccttatg aacaaggaaa tatttttaat catgatccgt
     8521 tgcgcacgag aaagttatta ttacacaaga aacaaatcgt tcgtcttgaa aacgaattga
     8581 aaaacactgg aatcactgtt gttcctttaa aagtctatat tcgtaacggc tatgccaagg
     8641 tattgattgg tctggcaaaa gggaaaaaat cgtatgataa acgggaagat ttgaaacgaa
     8701 aagatatcga tcgacaaatt gatcgaacat taaaaaattt ctctagataa tttcattatc
     8761 ctttttcgca tattcggtgc ttttgctttt acttaatgat aaaatttgtt agcatttaat
     8821 ctgataaaac atgattatca caggtttttt cttagatcag gagcaccgtg catggggaaa
     8881 aattcgcttc gtttttgact gaaataaaaa agtgtctaat tttcatgaaa atgttttgaa
     8941 ttttctgttt catggtggta tgataccgtg tggttagaaa ccgattggaa aaaaccagat
     9001 acgcacatga attgatttta tgtgaatttc ggggaaaaga ggtgaaatgg attggatgaa
     9061 cgctcgctaa cgtttcacat tggaccaatt tggtttgatg gtacagtttg tataatggtg
     9121 ttactgacct gtttgattgt tttcttctta gcttactttt ttacaagaaa tcttaagttg
     9181 aaaccaacag gcaaacaaaa tgcgatagaa tgggtcattg attttagtcg aggaatcgta
     9241 acagataatc tgcctagaaa agaattgagc aatttccatt tgttggcatt tacattgttt
     9301 ttatttgttt ttgttgcaaa caacataggt ttagtaacaa aaatcgtctt acctaacgaa
     9361 acaacacttt ggaaaagccc gacagcagac ccgtttgtta ctttaacttt ggcatttata
     9421 atgatcttac ttactcactt attcggggtg aaaaagttag gcttcaaagg atatttcgta
     9481 aattcgttct tgaaacccta tagttttatg tttccgatga aactgattga agaatttaca
     9541 aacttattaa cacttgcttt acgtctttat gggaatattt atgcggggga agttttgcta
     9601 accttgattg caaaaatgat gacaagctta ggctggttct cattaccatt agcaattcca
     9661 ttagagatgg tctggatcgc gttctccttg tttattggga gcatccaagc atttgtcttt
     9721 gtcactttgt caatggttta tatgagccat aagattgaag tagaagaata atactcggtt
     9781 aaaaaacaaa ctactattta tctattttag gaggaaaaca attatgaatt atatcgcagc
     9841 agcaatcgca atcatgggag cagcaatcgg agccggttat ggtaacggtc aagttatctc
     9901 taaaacaatc gaatcaatgg ctcgccaacc tgaaatgtct ggtcaattcc gtacaacaat
     9961 gtttatcggg gtagctctag ttgaagctgt tcctatctta ggggtagtta tcgccttgat
    10021 tctagttttt gccgtttaat tgaatcagta ttaaggcaga aacaacatcg tttgttactg
    10081 cttttttaca tggaaactag aaatactcca aagaaaggaa ggctcgccta tgctgaatca
    10141 attggcaatt gcagaagttg gcaatgcgat gttaggtaat atcatcgttg tcagcggctc
    10201 atttttgata ctactggctc tcttgaagca ctttgcttgg ggaccaatca gcgatatttt
    10261 gaaaaaacgt gaagacaaga tcgccaatga tttagattct gcagaacaat ctcgcatcaa
    10321 ctcagcgaaa atggaacaag aacgcgaaca acaattgtta gcctctcgtt ctgatgcagc
    10381 tgatatcatc aaaaatgcga aagaaagtgg agaattaagc cgccaaaata ttttgaagga
    10441 tgctcaagaa gaagcagctc gtctaaaaag caaagcccaa gctgatatca ctgtagaacg
    10501 tgattcagcg ctgaactctg taaaagacga cgttgcagaa ctctctcttc aaatcgcgga
    10561 aaaaatcttg aacaaagaat tatctccaga aatgcatgaa tcattaatca atcaatacat
    10621 tgaaggtcta ggttcttcaa atgaaactag ataaatatac agtaggaaga cgttacggga
    10681 aagctttatt tgaactggcg atcgattcaa atcaagcaga agaaatctat caagatctcg
    10741 taagtttacg tcaaatctat gaacaagtac ctggattagg gaatatgtta agtgatgttc
    10801 gtctagaacc tcatgaaaaa cgagaaatca tggacaagct tgtcagcggt tatgatggaa
    10861 tcgttaaaaa ctttttagaa gttgtttatg agtataaccg aatggatgac cttttgttca
    10921 tgattgaaga atatgaacgt cgatatgatg aacatcaagg gttactattg ggaagtgtca
    10981 caacagcagt cccactttca gacgaacaac gaatacaatt agaaaagaat gttgcaaaaa
    11041 caatggacta tcaatcagtt gaattaaaac aaatcgttga ttcttctatt attggtggcg
    11101 cgattgttga agccaaccat cgagtaatcg acggaagcat tcgcacgcaa ttagaaaaaa
    11161 tgcgtaatca attaaacaga tagtacaaag aggtaggtga atcgaatggc catcaaagca
    11221 gaagaaatta gtgccttgat taaagaacaa attgaaaatt atcaaaacgt gctttcagtc
    11281 gaagaaatcg gtaccgtaac ttatgtaggt gacgggatcg ctcgtgcaca tggtttagaa
    11341 aacgcgatga gtggtgagtt gcttgaattt tcaaatggct catacggaat ggcgcaaaac
    11401 ttagaatcaa atgatgtagg tatcattatc cttggcgatt tcgagtccat tcgtgaagga
    11461 gataaagtaa aacgaacagg aaagatcatg gaagttccag ttggagaggc cttgatcgga
    11521 cgggtagtta atccgctagg tcagccaatc gacggactag gtgaaatcgt tactgacaaa
    11581 gctcgtccag tagaagcaat ggcaccaggt gttatgcaac gtaaatctgt taacgaacca
    11641 atgcaaacag ggctaaaagc gatcgacgcc cttgtgccaa tcggacgcgg acaacgtgaa
    11701 ttagtcatcg gtgaccgtaa aacagggaaa acttctattg cgatcgatac gatcatcaac
    11761 caaaaaggtc aagatatgat ctgtatctat gtagcaatcg gacaaaaaga ttctacagtt
    11821 cgtacacaag ttgaaacatt gaaaaaatat ggcgcaatgg attacacaat cgttgtgaat
    11881 gccggtgcgt ctcaaccagc gccgttgctt tatatcgcac catatgctgg tactgcaatg
    11941 ggtgaagagt tcatgtacaa cggtaaacat gtcttgatca tttttgatga tctttcaaaa
    12001 caagctgttg cttatcgtga actttccttg ctacttcgcc gtccaccagg tcgtgaagct
    12061 tatccagggg atgttttcta cttacactca cgcttattgg aacgtgcagc aaaattaagt
    12121 gatgatttag gtggcggttc aatgactgcc ttaccatttg ttgaaacaca agctggagat
    12181 atctctgctt atatcccaac aaacgttatt tcgatcactg atggacaaat cttcttagaa
    12241 agcgacttgt tctacgcagg tactcgtcca gcggttgatg ccggactatc tgtatctcgt
    12301 gtagggggtt ctgcgcaaat caaagcaatg aaaaaagttg ctgggacttt acgcctagac
    12361 ttagcaagtt accgtgaatt agaagcattt acacaattcg gttctgactt ggatgcagca
    12421 acacaagcaa aactaaatcg tggacgccgt acagtagaaa tcttgaaaca aaaattgcat
    12481 gcaccgctag ctgttgaaaa acaagtagtg attttgtatg ctttgacaca tggattctta
    12541 gatagtatcc ctgtagacag tattttagac ttcgaacatg aattattcga atatctggat
    12601 accaatcatt cagatatctt tgaaacaatc cgtacaacaa aagatcttcc agaagaagaa
    12661 gcgttgaaca gtgcgattca ggaatataaa gacatgttct tagctacaaa aggaagtaat
    12721 tcctctacag aagataaatt gaaatccatt caaaatgcat agtgaggtga gatgaatggg
    12781 tgcttcatta aatgaaatca agacacgtat cgcttcaacg aaaaaaacga gccaaatcac
    12841 gcgggcaatg cagatggtgt ctgcttctaa gttgacaaaa tctgaagctt cttcacagaa
    12901 gtttcaggtt tatgcaacaa aagtaagaga aatcgttacg catttgactg ctactcagtt
    12961 aaatgatatt gcctcagaaa atcctagagg ggatatcaat tacaacagca tgctgctcag
    13021 ccgtcctgtt aagaaaactg gttatatcgt catcactgca gatggcggtt tagtcggtgg
    13081 atataacagc tcgatattga aacaaacgat gtctatttta gaagaagacc accaatcacc
    13141 cgatgattac gtgatgatcg caattggcgg aactggtgca gacttcttta aagcacgcgg
    13201 tatcaatcta gcatatgagc tgcgcaatct atctgatcag ccaagctttg atgaagtccg
    13261 caaaatcgta aatatggcaa caactatgtt ccaaaacgaa gtattcgatg aattatacgt
    13321 atgttataac catcatatca attcgctaac tagtcagttt cgtgtagaaa aaatgctgcc
    13381 aatctcagac ttagatccag aagaagcaac tactttcgaa caagaatata tttttgaacc
    13441 ttcaaaagaa gaaatcttgt ctcagttatt gcctcagtat gctgaaagtt tgatctacgg
    13501 tgcgatcgtt gacgccaaaa cagcagaaca tgctgctggt atgacagcaa tgaaaacagc
    13561 aactgataat gctgctaata tcattgatga gctgacggtt tcttataacc gtgcaagaca
    13621 aggggctatt acccaagaaa ttaccgaaat tgttgccgga gcttcggcac tagaatagta
    13681 ttgagattgg aggaaaaaag atgagttcag gcaagattgt tcaagtaatc ggtcccgttg
    13741 tcgacgtgga attttcttta gaccaatcct taccagatat caacaacgca ttagttgttt
    13801 ataaaaatga cgaaaataaa tcaaaagttg ttcttgaagc agcgttagaa ttaggggacg
    13861 gagtgatccg gaccattgcg atggaatcaa cagatggttt acaaagagga atggaagtca
    13921 ttgatacagg caaagcaatc tctgttcctg taggtaaaga aacattaggt cgcgtattca
    13981 acgtactagg agatacgatc gatttagaaa cacctttccc agaagatgcg gaaagaagcg
    14041 aaattcataa aaaagcacca gcctttgacg aattaagtac aagtacagaa attttggaaa
    14101 cagggatcaa agttatcgat ttgcttgccc catatttaaa aggtgggaaa gtcggactat
    14161 tcggtggtgc cggtgttggt aaaaccgtac tgatccaaga actgatccat aatatcgccc
    14221 aagaacatgg tggtatttct gtatttaccg gtgtaggtga acgtactcgt gaaggtaatg
    14281 acttgtatta tgaaatgaaa gattcaggag ttatcgaaaa aacagccatg gtgttcggac
    14341 aaatgaacga accaccaggt gcacgtatgc gtgttgcttt gactggtttg acgattgcgg
    14401 aatatttccg tgatgtagaa ggtcaagatg tactgttgtt tatcgacaac attttccgtt
    14461 tcactcaagc tggatctgaa gtatcagcct tgttaggacg gatgccttct gcggttggtt
    14521 atcaaccaac attggcaaca gaaatgggtc aattgcaaga acgtatcaca tctacgaaaa
    14581 aaggttcgat cacatcgatc caagcaatct atgtgccagc cgatgactac actgacccgg
    14641 cgccggcaac agcattcgcc catttggatg cgacaaccaa cttggaacgt aaattaacag
    14701 aacaagggat ttatccagcg gtagatccat tggcttcttc ttcaagtgct ttagcacctg
    14761 aaatcgtggg agaagaacat tataaagtag caacagaagt ccaacacgta ttgcaacgat
    14821 atcgtgaatt acaagatatc attgctatct taggtatgga tgaattatcc gatcaagaaa
    14881 aagtgttagt ttctcgtgct cgcagagtcc aattcttctt gtcacaaaac tttaacgtgg
    14941 ctgaacaatt tactggtctt ccaggttcat atgtccctgt agaagaaaca gtgaaaggat
    15001 tccgcgaaat cttggaaggc aaatacgatg atcttcctga agaagctttc cgaagtgttg
    15061 gccgaatcga agacgtcgta gaaaaagcga aaacactagg ctactagaaa ggggtgcccc
    15121 atggatcaat atttaacagt aaatgtggta actcctgccg gtttggtcta tgatcaccat
    15181 gcagctatcg tcgttgcacg tacgacagct ggtgagcttg ggattttgcc taaacatgca
    15241 ccaatcatcg ttccattgac gattgatgaa gttcgtgtaa aacgaacaga ttcagatacc
    15301 cacgtcgatt ggatcgctgt taatggcgga atcatggaag ttcgtgataa tgttgtttca
    15361 atcgttgctg atagtgcgga acgtgaacgt gatatcgatg tatctcgtgc ggaacgtgca
    15421 aaacaacgcg cagaacgcca aatcgaagaa gcaaaagcaa aacaagatac gaacgaattg
    15481 aaacgggcaa ctgttgcgtt gcaccgagca atcaatcgta tcaaagtatc taagcactca
    15541 taaaatgaga tgcggatgtt tatccgtgtt taagaacagt aaaaagaggt tatgacagaa
    15601 gtggtcagct tcaagacata agaaggaatt tacgaaaatt gcttctcaaa tttttgtgaa
    15661 atctcagctt atttttcgaa gaagctactt ctggaacacc gtatatcaga ggttgtgaaa
    15721 tcagcgtttt gtcctgagta ttagagaaac aaatggaaaa tagttcctca tattttgccg
    15781 ttttttgagc taataccgag ggtcagatga ttgaacaccg tttatttgga ataagggact
    15841 gcgacaagac ttttgtctca gtcccttttt ttgcatattt ttagcattta tttcctaaaa
    15901 aatgtaaggg gattcctata tttttaagta tacttgtttt tccttttata cattttatgg
    15961 tattatcatt ttattagtta ttataaaaca atcgttagaa aggacgtcat tatgcaagta
    16021 tacggaattg atgcggtgct tcgaatcgtg agtcatttgg cgtttatcta cttagccttc
    16081 tggtctttac gttcgttacg cattgaaact tttttcagac caatgcataa tacgcaaatt
    16141 agaatggcaa tcgtactttt ttcaattttt ttagggtaca ctgcaagtaa ttttttctta
    16201 gaagttatcg ctttatgccg aaatcttttc gttagcctgt aaattttaaa aaaaggtact
    16261 attttaaaag caaggatgtt ataatagaaa agattttgag taagtgtatg ctcattggaa
    16321 ttttttggag ggaacaacat ggaagagatc atcgtaagag gtggcaatca attaaatgga
    16381 acagtaagaa ttgaaggagc taaaaatgct gttttgccta ttttagctgc tagtttgtta
    16441 gcagaagaag gtattacgac attagacaac gttccgattc tttctgatgt cttcactatg
    16501 aatcaagtga ttcgtcactt aaacgtagat gtagattttg atgaacaaaa gaatcaagta
    16561 acaatcgacg cttcaagaca attagaaatc gaagcgcctt atgagtatgt tagtcaaatg
    16621 cgtgcttcaa tcgttgtgat gggtccgcta cttgccagaa atggacatgc aaaagtagct
    16681 atgcctggtg gatgtgcgat tggtaaacga ccaatcgatt tgcatttaaa aggatttcaa
    16741 gctttaggag caaaaattat tcaaaaaaac ggctatatcg aagcaattgc ggatgaattg
    16801 atcggtaaca cgatttattt agatttccct agtgtgggtg ctactcaaaa tatcatgatg
    16861 gctgccgtaa aagcaaaagg aacaaccatc attgaaaacg tagctcgcga accagaaatc
    16921 gttgatttag caaacatttt gaataaaatg ggcgctcaag tctatggagc tggtactgaa
    16981 acaatgcgta ttgaaggcgt ggatcattta catgcagtca accactcaat cgtacaagat
    17041 cgtatcgaag caggtacctt tatggttgct gctgcaatga cacaaggcaa tgtgctgatt
    17101 gcagatgcta tctctgaaca taactgccca ttgatttcta aattgatcga aatgggtgca
    17161 gaaatcattg aagaagaagg cggagtccgt gtcatcggac caaaacatat cttacctaca
    17221 gatgtaaaaa cgatgcctca tcctggtttc cctacagata tgcaagctca aatgacagct
    17281 atccaattag ttgcagaagg tacaagtgtg gtgactgaaa cagtgtttga aaatcgtttc
    17341 caacatttag aagagatgcg ccgtatgaac gctcatgtca aaatcgacgg aaatgtagca
    17401 atcatggatg gaaaccatga attgcaaggt gcagaagtat atgcaacaga cttgcgggca
    17461 gcagcagcat tagttttagc tggcttgaaa gcaaatggaa tcacacgtgt gcgtaacttg
    17521 aattatctag accgtggata ttataatttc catatcaaac tacagcaatt aggtgcagac
    17581 gtcgaacgtg tagacatgga tcaaacttct gctgaaaaaa cagcacaaac gattgcttaa
    17641 ggaagtggct gaatgagtaa ccaacgatat atcttattaa ctttgataaa gattttagtg
    17701 gtgatcttat tgcttatcct tctttttgtg gccggaacga tgatcggata tggtgtcatc
    17761 ggtggcggca atccatttaa agtattccag ccaagtttgt ggattcatat tcgagacttt
    17821 tttcattgat aaataagaat cacagtggtt ctagtcgata tgaagtgagc catgaagcag
    17881 aacatgataa aagaaaacag aagggaacgt tgacgttagt cgcgatccct ttttttagta
    17941 aaagaaaaga aggaatctaa ttgatgaaga agatattgat ttatttagtt agaggatatc
    18001 agcgctttat ttcgcctgca tttccaccta gctgccgtta ttacccaacc tgttccaatt
    18061 atatgataaa agcaattcaa acccatggtg cgctaaaagg gacggtaatg gggactgcac
    18121 ggatcttacg ctgtcatcct tttgtcaaag gcggtatcga ttatgtacca ttgacattcc
    18181 gattaacgcg aaataagaaa gataaaaaag aaccagatta taaaaaggtt gtgaaaaata
    18241 cgggtagcac tgagcgataa agaaaaagag gaaatttatt gtcaaagtaa caactccgca
    18301 actacttata tgggggacaa aaaatgatcg aagaactaaa caagaaagat ttaccatggt
    18361 ccttgttatt agatgcagat cctgataaag aaaaagttca agtatatgtt tccagaggga
    18421 gtggctggat atggaaagaa aaagaaaaaa caataggtgt actgatctat gtggcaaggg
    18481 aaaaagaata cgaaatcgtc aatgtagcag taaccccaac gtatcaagga aaggggattg
    18541 gcggaaaatt acttgagaca gcttttcaga agctttcaca gctagcttca aaccaaactc
    18601 ggatcattat acgtactggc actactagta gcgctgcgtt acacctctat caaaaaatgg
    18661 gctatgtgga gattggtcga gtcaaagact attttatcca tcattaatct gagcccattt
    18721 ttgaaaatgg agatcaatta agggatcaag tgattttagc aattcactta cctaaaaata
    18781 gctttaacta ataaactttc aattcgagaa aatgctttca aatgctaaat agatacatta
    18841 caatagcttt agcaaacaaa aaggagaata tttttatggg aatattagtt tttgattttg
    18901 gtggatcggc tgtcaaatat ggttgttggg atggcaaaga gattaaaggt aagggacaat
    18961 ttgcaacgcc tgaatcttgg gaagaaatga aaacgcaatt atttcaagtt tataaaaaaa
    19021 tactcgtttc ttttgaagtc gaaggaattg gaatcagctc tcctggagta gtgaataatg
    19081 agaaacaggt gattgaggga atcagtgcga ttccttatat tcatggattt aatattttcc
    19141 gagatttgga aactctgttt caactcccag taacgattga aaatgacgcc aactgtgccg
    19201 gaatggctga attttatgaa ggtgctgccc gtgattatca agatgttgct tttgttgtgg
    19261 caggaactgg gattggcggg gcgttgttca cgaaaggaca aataaataaa ggggcacatc
    19321 tttacggtgg agaatttggg ttgatgttct tagatggcga taagacattc agtaaacttg
    19381 gtacagcggt acagatggct tggcgttatt gtgaacgcaa aggtctcgat aaaaatgctt
    19441 ataccggaaa agatgtcttt gaattagcag aaactggaga tgcaatagca aaagaagaag
    19501 ttgagagttt ttatacgtat cttacaaagg gattattcag tattcaattt tcgtttgatc
    19561 cagaagtgat cgtattaggc ggcggggttt ctgcaaaaga agggctcatt gatgagatca
    19621 aaagccgaat gaagaagctg acagaacaat tcgatctgca tgatttcgaa ccgcagattt
    19681 tattatgtga gtacagaaac gatgctaatc ttgtaggtgc agcagcaaac tatctcgctg
    19741 tacgtcaagg agagaattaa gagctttaga gaaggtgcag cttgcataaa agaagagaat
    19801 gaaatgaata ggagattttt acctaaggaa tgttttattt gctgcagtat gataaaatat
    19861 ctgagaggtg aaaatgatgt caaaaaaaaa taaagatatt gaagtgcgta tagaagaagt
    19921 gaaaaaagat atcaaaggta agaattatga agtcactcag ttatttatag ggaaaaaaat
    19981 gattggtgag atcttagcct atgggccaaa agaatatgaa attttctttg gtgaagaaga
    20041 ttttggaaaa gaaaaaagtc ttgaaaatgc gatagaaaca gtgattcgac actggaatct
    20101 gcacgaataa aaaaatatca aaaaaacttt caataaaggg ttgcaaaaat ctggaaaatt
    20161 aggtattata actaagtcag gttgatacaa acctttcgga ggagtagcga agtggctaaa
    20221 cgcgacggac tgtaaatccg ttccttaggg ttcagtggtt cgaatccact ctcctccatt
    20281 tcattggggt atagccaagc ggtaaggcaa gggactttga ctccctcatg cgttggttcg
    20341 aatccagcta ccccagtata
//
LOCUS       DACDPS010000042        20197 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.042, whole genome shotgun sequence.
ACCESSION   DACDPS010000042 DACDPS010000000
VERSION     DACDPS010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.042"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..456)
                     /locus_tag="IWJ07_001665"
     CDS             complement(<1..456)
                     /locus_tag="IWJ07_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP9333246.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEK"
     gene            complement(561..1424)
                     /locus_tag="IWJ07_001666"
     CDS             complement(561..1424)
                     /locus_tag="IWJ07_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP9333247.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(1620..3719)
                     /locus_tag="IWJ07_001667"
     CDS             complement(1620..3719)
                     /locus_tag="IWJ07_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP9333248.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(3939..5048)
                     /gene="rpoD"
                     /locus_tag="IWJ07_001668"
     CDS             complement(3939..5048)
                     /gene="rpoD"
                     /locus_tag="IWJ07_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAP9333249.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(5072..6913)
                     /locus_tag="IWJ07_001669"
     CDS             complement(5072..6913)
                     /locus_tag="IWJ07_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP9333250.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(7095..9488)
                     /locus_tag="IWJ07_001670"
     CDS             complement(7095..9488)
                     /locus_tag="IWJ07_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9333251.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(9595..10455)
                     /locus_tag="IWJ07_001671"
     CDS             complement(9595..10455)
                     /locus_tag="IWJ07_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAP9333252.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(10514..11035)
                     /locus_tag="IWJ07_001672"
     CDS             complement(10514..11035)
                     /locus_tag="IWJ07_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAP9333253.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            11355..12074
                     /locus_tag="IWJ07_001673"
     CDS             11355..12074
                     /locus_tag="IWJ07_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9333254.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            12100..14691
                     /gene="mprF"
                     /locus_tag="IWJ07_001674"
     CDS             12100..14691
                     /gene="mprF"
                     /locus_tag="IWJ07_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP9333255.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(14879..16066)
                     /gene="tuf"
                     /locus_tag="IWJ07_001675"
     CDS             complement(14879..16066)
                     /gene="tuf"
                     /locus_tag="IWJ07_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP9333256.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            16434..16790
                     /locus_tag="IWJ07_001676"
     CDS             16434..16790
                     /locus_tag="IWJ07_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9333257.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(17031..18035)
                     /locus_tag="IWJ07_001677"
     CDS             complement(17031..18035)
                     /locus_tag="IWJ07_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAP9333258.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            18201..19052
                     /locus_tag="IWJ07_001678"
     CDS             18201..19052
                     /locus_tag="IWJ07_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP9333259.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            19069..19314
                     /locus_tag="IWJ07_001679"
     CDS             19069..19314
                     /locus_tag="IWJ07_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333260.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            19501..>20197
                     /locus_tag="IWJ07_001680"
     CDS             19501..>20197
                     /locus_tag="IWJ07_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333261.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYSLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAIT"
BASE COUNT         5699 a         4144 c         3542 g         6812 t
ORIGIN      
        1 tttttctttt tcatagcatt cctgacagat tccatggaac gttagccgat gatcttttac
       61 taaaaaatga taacgttctt ctactacttg ctccacttca agtaaaagat cctcttctac
      121 ttcctcgatc gtgccgcagt tcaagcatag taaatgatga tggaaatgtt tggcgccttc
      181 ttttctcaaa tcataacggg ctaatccatc attaaaactt actttatcga caattttcaa
      241 ttctgtcaag atttcaaggg ttcgataaac agtagcaagt ccaatttccg gactcttttt
      301 tttcactaaa aagaagattt cttctgcaga taaatgctct ttctcatttt ccaaaagcac
      361 aagcagagta gcttctcttt gcggtgtcag cttaaacccc gcttcatgta gctgctgttt
      421 tgtttttttc atcgccgaaa tagcattcat gaccatttac tcaaccccaa caatttataa
      481 taattacaaa taaaaaaaga tataacacta tgttaattaa aattattata agctagttaa
      541 gaaaaacatg caagggattc tcaattagat tttttgacta gttttgtcac accgttttct
      601 tgtgtaatca atccttgttt caaaagatta cctaatgcgc gcttaaatgc tcctttgctg
      661 ataccaaaag ctttcatgat ttcttctgga tccgatttat ctgtaaagtt gatttgctga
      721 tctgcagaac gttctaaaat ggttaaaatc atttgtgcat catctgatat agcttcatat
      781 gctcttggct tcaatgaaag attcagtaca ccgtctggtc ggacaccaat cactcgacct
      841 tttatctgct cgcctagacg aggttcatta tatcgttcag aaggatggat aaacccaaga
      901 tagaaatctt ccgttaaaac gtacgtacct gttaatttca agcggtatgc tgttcctatc
      961 acatctttgt ttttcatttc ttctgttcca tatttgctta aagatttgaa tattttttca
     1021 tctgctaaac tgccccaaat acggtctttt gcatcaactc taagggaaat catcaagcga
     1081 tccccttttt taggccatag ctctgtcatt gtcggcaatt catcaagaga tacgacaaag
     1141 tctttatctg gaagaccgat atcgacaaac gcacctaaat cttttcgtgt agcagtaacg
     1201 attccaaaag cgtaatgacc gatacggctt ttaggaattt gtgtagtgat ccgattttca
     1261 tgtttttgat tttggtaacc gaatccttca actgcttcac cgattttgtg ttcaccttct
     1321 gatttgctta agccaaaggt ttgtccgttt ttttggacaa agtagttttt ttcattttca
     1381 tcaataataa tacctgtaaa tatagttgct aataaatcgt tcattttttt ctccctctat
     1441 agtgaaaggt tttcttaata catttctttg ctatcataac ataatgttat ctacttgaat
     1501 agctgagcct tggtttggaa aggtgaaaaa aggagtatga caacagttgt gtcatactcc
     1561 cctttttcga ataaacggtg ttaaacggca ggctcacagc ctctttcatt tgcctaaaat
     1621 taattgctcc catttcctcc gaaccatcca gttttacagt tgatggttac gatgtaaaat
     1681 ggcaaagcac tttctccaat gcctttcatc aatgcagatt ttgttgaagt ggcatataga
     1741 ttataatagc cttccccatt ttcgattctg gctttttcta atctgtaacc tccttcaacg
     1801 atatatccac gttggataca tgtagcaatc actttgcttt ggacacctgg tgcccacgtc
     1861 catgctggat tagatgcatc ggcaatatca cttgctctgg cagatttgat tggctgattc
     1921 gctgagtttg aagaggaagg ggtcgaggat gccgcttctt gctgtgctgc ttgtgcgacc
     1981 gcagcttgtt gagcggcttg agctgcagct gctgcttgag cggcttgttt tgcttgttct
     2041 tcagctgctt ttttcgcttc ttctgctttt ttcttttcct cttcttcttt tttctcttta
     2101 tcagtgagca tcttatctac ttgtttcaat tgttcattca actgagcaga tagatctgta
     2161 tttttcactt tttctactgc ttcttttgcc gtcttgtatt ggtcgcgtgt tgctgaatcc
     2221 actacttttc cgtctttaaa gactatagct gttttttctt tagctgtttg caattcagca
     2281 tattgatttt tagcttctgt ttgtgcttgt tgaatcaatt ttccaaaatc tgtgttatcc
     2341 gtttcgattg cttggatctc tttatctgct ttcaacttga tgtcatctgc tagatgatca
     2401 tcgacgatgg caggtgctat gaaccactca ttcacttttt ggatatctcg gatcttagat
     2461 tggatatctt ggtaagttgt ttctaactct gaatatcctt tttcattctt aagactggat
     2521 aaatcatttt ttagttttga taaatcttga tgaatcatat ctgcacgaat aaattgatgg
     2581 tgatcatctg tataaaaggc agcaagtttc tcatgaagtt cttccatttg ttgctgtctt
     2641 tttgcttctg ctgctacttc ggcttctact ttttgatgat ggtcataata agcccagctt
     2701 cctccaccga gtagaagtac tgctgctaaa acgagataag gtactttttt tgattttttc
     2761 ttttgcgctt tatctggatc tgttttttct aagtttgatt cttgatgtgt tttttcagta
     2821 tctggcacga ttgttattga tttcgcttct gcttcaaaca gcttaggctg gttctcttct
     2881 ttttctattt ttgcttcaag atcagtaccc tctttgctta cagattgatt ttcagcatcg
     2941 gattcactat ttatttcatc gggctctgtt gactttttgt ctgtgttttc gagtatttct
     3001 tttttctctg cttccggcag tttattcgtt ttttcctttt ctaattgttt ttcagtaaat
     3061 acttcagttt tttctgtgat atccgttttt acgtctttta tttttactgt ttctttacct
     3121 gtggcgtttt ctctatcaga gtctttttca gtttcttgtt ccttttctgg ctctactttg
     3181 ttttcctgtg caattttttc actgatcatt ttgagcagct ctttcgcgtg ctcttctttt
     3241 gtatcttcac gatgtgcttt gatataagcg gatagtatcg aattttcttc ctcattatcg
     3301 acgatttctt tcagttcagc tactcttctt ttttgttttg attcaagcgt ttcttcttga
     3361 ttctttcctg attcttccga agtctcatga acagttgctt tcttctgttc atttttctct
     3421 atattagtta cttgatcgtg agcaggtggt tcttcgtctt ctggttctgc tttttcgaat
     3481 aattccatta cagattctag aggtaaatct ttcaattccg accattcgat tggatcgttt
     3541 aattctgtat caggaaagac tgcatcttca gttgatttat cctgcatagc tgcatctgta
     3601 tttgtttctt caattgattc ttttatttgc tgatcttctg cagttatttg atgaccgcag
     3661 tctggacaga tagtattctg ctccattttt ttcgatccgc aatttggaca ttttttcatc
     3721 acttagcgat cggctccttt actaggttta cgctaccatt taatctatca tattctatac
     3781 gtcacgccaa tagcttattt ttaggattta cttaaaaata atgaatgaat ttccttccta
     3841 tagaaaaaaa agcttgagac tgcgtagtct caagcttttt ttcttttaaa ataaaccaaa
     3901 cagttttact gatttctctt ctggtcaata gtccacgctt attctaagaa atcttttaat
     3961 tgttttgaac gagaaggatg gcgcaatttt cttaatgctt tagcctcgat ctgacgaata
     4021 cgttcccgag taacaccaaa tactttccct acttcttcca atgttctcgt ccggccatca
     4081 tctaaaccaa aacgtaagcg aaggacattc tcttcccgat cagtcaatgt atctagtaca
     4141 tcttccaatt gttcttttag taactcataa gctgcgtgtt ctgctggact tgttgcttcc
     4201 tggtcttcta tgaaatcacc taagtgagag tcatcttctt caccgattgg cgtttcaagg
     4261 gaaacaggtt cttgcgcgat tttcaagatt tctcggactt tttctgttgg aagatccatt
     4321 tccgcaccga tttcttctgg cgtaggttct cttccaaggt cttgcagcaa ttgacgttgg
     4381 atacgtatca atttattaat tgtttcaacc atatgaactg gaatacggat cgtacgtgct
     4441 tggtcagcga ttgctcgagt gatggcttga cgaatccacc atgtagcata ggttgaaaac
     4501 ttaaatcctt tgcggtagtc aaatttttca actgccttca tcagtcccat gtttccttct
     4561 tggatcaagt caaggaactg catgcctcgt cctacataac gtttagcgat acttactact
     4621 aaacgaaggt ttgcttctgc cagacgctgt ttcgcttctt ggtcgccttc ttcgattttc
     4681 aatgctaacg ctacttcttc ttccgcagtt aaaagggaga cacggccaat ttctttcaga
     4741 tacatacgaa ccgggtcatt gatttttaca ccggttggcg cagaaagatc ttctgttttt
     4801 gcttgttcag cttttttctc agcactcttc aagctgtgaa ttgatggatc accattttca
     4861 tccaccacac tgatacctgc gtcttcaatc ttttggatca atttgtccat ttcatctgca
     4921 ttcaaagtga atggtgtagc aagctgattg gataaatcat catatacaac ttgtcgtttg
     4981 ggtttgtttt ctttgatgaa agctgctact gctgcttcat acgttttttt atctgttcca
     5041 tttgccataa aagaaggccc cccttgagta gctatttggc ttgttttaac tgtttcgtta
     5101 aattgatgat ttcaatcgtc aattcaagct caagctgttt gtttccagtt cgtctggctt
     5161 cttgctgcat ttgttttttt aatgttattt catcagctaa gccagatttg ctgatcacat
     5221 ctagcaagtc ttggatttct cgtggcgaac tttcttctgc caccggtaaa tattcgattt
     5281 ccaccaccaa ccgccttaaa ttttcatctt gcaaaaagtc taaaaactct gcaaggttga
     5341 attcataatg ttgtgagaca tatgcatcga ataaaaaata aacttctgca tatgcatcat
     5401 gaataaactg aaattctcgt tcttttaaaa tattacggac agaaacttct ttaaatgcac
     5461 gatataatag aatctgctct gccttttcta cttgcgtcaa aggtctcttt ttccgtgcag
     5521 tttcttgtct cgtaacctga gtgtgttgac tttcctgagg ttcttgacga ttttgctgat
     5581 ttgtccttcg aagatcgcgc agttgttttt gtatcgtctc tctgccgatc tgaaactctg
     5641 ttgacagttg agatagatac atatcttgtt cgattggtga aacaactcgg ctaagttcaa
     5701 ctagtaattc ttcgaggtat tcgatcttgt ctttttcatt atctaaattt ttcccttgtt
     5761 ttaaataacg tgatttaaaa gtaaagactg tttcgcgccc atgagccact aattcttgga
     5821 aagatgcttc tccatactta cggagatatt catcaggatc aagcttttct ggaacgacta
     5881 cgacagaaag tgctaatcga ctgttttgcc gaagcaattc aatccctcga ttggttgctt
     5941 caaaacctgc gttgtctccg tcatagcaaa agaccaattc tttcgtcagc ttttccattt
     6001 gttggatctg ttgagctgtt agacttgtcc ccatagaagc taatccagtt ttgataccag
     6061 cctgccatgc agcaatcaca tccataaacc cttcgaacaa caaaatttct ccgtttttcc
     6121 ggatatcgct tcttgcttta tcaagattaa ataaaacttg tcttttattg aacagttctg
     6181 tttctggact attcaagtat ttgggctgat ctttttcctg ccctttttca ggtgcaagcc
     6241 agcgtcctga aaaaccgatc gtctggcctt gagaattacg aagtggaaac atcactcttt
     6301 gataaaaacg atcagacata cggccatcat catgcgtgac aaacaatcca gagcgttcta
     6361 acaattcatt tgaaattgat tcattcttga atacttgctg aagaaaagaa cgctcattag
     6421 gtgcaaaacc tatgttgaat tcttcaatta attctaaaga caacccacga ctttgcaaat
     6481 attctaaagc tggctgtcca gcttttgtat gcaatagcat gtgatgatag atttccgctg
     6541 ctttttcatg agcagcaatc agtttccccg tagcagattc aggattttgg acagctatag
     6601 tacgctgacg ccattgatct tctaacggta tctgttcgaa gtctgccact tttaccactg
     6661 cttctggaaa acttagacct tctagttctt gaagaaaagt aaacacgttt ccgcctttac
     6721 cacaaccaaa gcaatgaaaa atctgcttgt cttctgcaac agaaaaagac ggtgtacgtt
     6781 cttcatgaaa aggacacagc cccaagtaat ttttaccaga ctttttcaac tggacatatt
     6841 gtccaatcac ctcaacgata ttcgtttgac tgcgtactgt ttcgataacc tcttgaggaa
     6901 tccgttgagc cattttttca ccccctgctc tattgatatc ttttgggttt aacagaagat
     6961 atttcaggag aataaccggt aaaaaatcgc actaactgaa atcagtgcga ttttaaagga
     7021 cacaattata catgtttgat tgtatcatat ttcattgctt tttcaagtat aacacatttc
     7081 tatctcatta agcatcaatt tgctactctt ttcagtaact gattttgaat atcagccttc
     7141 cagtacaata ctcgaagaac aatatttacg agcatgaata caaagaagta tcctacgctc
     7201 tctcccaacc ctaagtttac ttccaaaatt ccatggaaca gcattgcagc tacatatatc
     7261 cctacgatcg tcgttactgc taggaagatg cgcaatgggt tgaagggata acaagctttt
     7321 acaactgcca tgcaactgat cccaatcagc aagtaataca tcaaagtagt agtttcagca
     7381 tttccccatg cgaatgtttt tccaagtaga tacaccgcaa tgatattcac tactacaagc
     7441 aaggcatttg gtaaagcatt aataagtgct gtaggcaaat aacggtaagt gatttttctt
     7501 ttatcttgtt caaaagataa aaagaaggaa ggataccctt caatcgctaa atcaatcagc
     7561 gtgatttgga tagggataaa cggaaatcca attgctgtaa cggcacatat aatcgataaa
     7621 ataaatgaat aaatcgtttt gataaaaaag atgcctgaga ctttcgtcac attgttgacc
     7681 actcggcgtc cttcaaacag gacttctggc aatgtagtaa aatcagagtc caacaatacc
     7741 agattcgcaa tctgacgtgt tgctccatca ccttctgcca tagcaatgct gcaatctgct
     7801 tctcttaacg ccaaaacatc attcacaccg tcgcctgtca ttgcaaccgt tctgccgctt
     7861 tctttcaatt catttaccag caatttcttt tgctgcggac tcactcgtcc aaaaaccgta
     7921 tagcgatgag cagcttctcg cacttccatt tcttcagtaa tttgagaaag atcgacatac
     7981 gcatcataat tcggtaatcc tgcacgtcta gcgatattgg agacagtaaa aggattgtct
     8041 ccagaaatca ctttcagatc cacaccttcc tcatgaagat aagctagtgt ttcttgagcg
     8101 ttttttcgga ttggatcatc gatttcaaac agagcaaccg gctcaacaaa tgctggtttt
     8161 tcttcgttca attgctgatt tggagcgatc cccaacataa gtacgcgata gccattcgct
     8221 tgggctgttt ttagactgtc cggaagccga tcttcttcca ccaaacgttc aggcgctcct
     8281 acgtacacga ctcccagttc tggaaattcc atcgcgcccc atttgcgatc agaagaaaat
     8341 gcgactttgt ttgtcacatc ataccgatta gaaagctcaa aataatctct catagcccgc
     8401 atagtgatgt tgttatcact gctttctgcc agatacgtcc ccataatttc tgggatggct
     8461 ttctcgtaca gcggatgtag tgcttctacc cgctggatac tcattttccc ttcagtaatg
     8521 gtccctgttt tatccaagca taatgtatcg acatgggcca gcgtttcaat tgaatacatg
     8581 tcttgaacaa ggattcgttt ttttgccagt ttggttaccc ctgtcgttaa tgcaatactt
     8641 attagcaata ccaaaccctt tggaagcatg cctaacaaag ctgctgcaga tgcaataaca
     8701 gaaattttga tagaagcatc gcggatgaac agtgcttcca aaaatagtat gatccccaaa
     8761 ggaataatca cgaaactagt gaatttggat actttgcgaa tcgaatctac aagttcagaa
     8821 cgaatagctt tgtgagtctt tgcctcgcta gtcaagcgga cagcataatt atctgcacct
     8881 actcggataa cttcagccat gacagaaccg cttgtcaaaa agcttccaga aagcaattca
     8941 gctccagttt ctttgacaat caaatcagat tcgcctgtca aaagagcttc atttgcttct
     9001 gcccgtccag atataacctg taaatcggct ggtatctgtt ctcctgctcc gatttttacc
     9061 aaatctccta ataccagctc tttagtagaa atcgattgtt ctttaccgtc ccggatcact
     9121 agtacttctg attgggaaac aatcgataat ttccgtacaa gatttcttgc atgtatttct
     9181 tgataaattc caataacgat attgacgaca ataattgcta aatacgccat gttggaatac
     9241 gcacctacaa ataacagaca aatcccaata ccaaagttca gaaaattgaa taacgtcaat
     9301 atattatcgc tgaatatttg ttttgtactt tttgatgcat cttcctggta gtcattttgc
     9361 tggccagaag ccattttctg aactacctct tgctgtgtaa gtccattcat tttctttttc
     9421 tccattttcc gccaactttc aaaaataatc acgtgtttaa gtctagcgtg ttccaagatt
     9481 aaaaacaact tgtttttata aaatttatag aaaaacacac gaatgatttt acaaaaaaga
     9541 tgccaacctt tttgtttgaa tcacccgtgt gctgttttta ttatcaatct atctttactt
     9601 atattgttcc tccaaacggc tcatccacca acctagtgct gtaccggcta taaaaagaac
     9661 aggacaagct agtaccccag caactcccaa tcccgcgtaa tcggtaggaa tgatcataat
     9721 cgttgaagca aaaactacac ctaaaatgaa atggaatagc ttgctatatg cctttgagaa
     9781 aatgtaatcc atgatttttg acaatccaag aacacaaaga atcccaccaa ttccgatcgg
     9841 cacaatcact tcaagtcgta cttctttaaa tccgtcagcc atcgctttat acaatcccat
     9901 atacaccaaa aaattagaag ggctcaatcc tgggacgatc atacccaaac caatcaatcc
     9961 gccggcaatc agccatgttc caaaattctg atcgacttgc ccgtcaaata attgagcgcc
    10021 gtataataaa aacaaataag ccagaacaaa agtaacaacc atgatgatca catcagaagt
    10081 atctcttcct tttttccctg cttctttcca taacgcagga acagttccag cgatacatcc
    10141 gacaaaaaac cataatatcg ttgtttcata cgcacctaat aaaaaactga ctaaaaaaga
    10201 aagtagaaag atgcctgtca ctccgcctag accaacaggc agaaaaaaca aaacattttc
    10261 tttgaaattt tttgtaaggt gggctaaaaa agaaatcagc cgctcataca tgccaaagac
    10321 agctgctaat gcaccaccgc tgactcctgg caagataaat cctgaaccga taaacattcc
    10381 cttcacaaac cgtaacatcc agtctttggt tgtgtacccc acttcttgat tttccatttt
    10441 ttgccttttt tccactattt gcgcactcct tctttcattt aattttattc atgattgaac
    10501 atttcctgct attttactta aagacttttt cataggtctc tattattttt tcattcattt
    10561 tgacaatatc aggataaaac atgttacctc cattatgata gagatagccg ccattgtaaa
    10621 gcaaggactg gatccgccaa tatcggtatt gtttcccttc ctcatttcct aaaagtgggc
    10681 tcagcacatc tttagaatac gcttctgcta attctactgt gttttctccg ccatgatttt
    10741 ttacataggc aatataatct ttcccaaaat tatatgcttg gacagctgtc cataaatcgc
    10801 atccttgttt ctttgcttct actatcattt ccgccaaata tttgacacct tgatcaatac
    10861 tgtcagctgg attatcaatg atctcagctt ctccatgcag actttccgag ctctgcatga
    10921 gatctgtccc ttttcctttt gattcggtca atattatcgc cttgatcaaa ttctcgtagt
    10981 tttccatatg ataatttttt gcagctgttg ttatttctat ttgatagctt tccactcgtt
    11041 ttaccgaacg ataagttctt cctcataaaa acaacaccgc accaataacc gtcagaatga
    11101 tcatgtaaaa aagaatacgg ataatagata atcgttttcg cttcatttca tcacctagtt
    11161 attttgacat ctttttttat cttactattt ctttatcttc ttggctattt cttccttgat
    11221 ttctgtgtac gccttaagaa aaaatgaatt ttcctgtatt ttttgacatc ttacgaaaat
    11281 gatcgttgtt tttcagtaaa aacaattgcc caatgactga tataatcgtt atactttaca
    11341 cagtaggtga ttatgtgaaa aaagaaaaga aatatgcaaa aatgccagat gggagcgaaa
    11401 tttactatga aaagagcggt caaggcttcc ctctcttttt actgcatggc aatgacggca
    11461 gcggccgctt tttttcagaa caggtccctg tactcgaacg ctactatacc gtgtatctag
    11521 tagatagccg aggacatggt cgttcgacaa acgaagcatc tatgttgaat tttcagttaa
    11581 tggccgagga tctgaatacg atcatgttgc tggaaaaaat cgatcaggca gattttcttg
    11641 gatttagtga tggagcaaat ctagcactgg tatttgccag cagcttccct aaaaaagttc
    11701 atcgattgat tttaaattct ggaaacacgc ttgttaaagg cgttcgtttt tctgccagag
    11761 tcatcagtaa tattcattat gcatgggtat ggttactctc tttatttcgc ccaagtcttc
    11821 gcaaaaatct tttagttatc aaacttttgc tgcatgacat cgggcttaca gaaaatgatt
    11881 taaaaaaaat caactccccc actttgatca tcgtgggaaa aaaagatgta atcaagctaa
    11941 aacattcttt gtatatagct aaaactatac caaaagcttc ctttgtgctt gtgaaagaac
    12001 aaggacatga attagctaga aaagatcctg aacgcttcaa tcgagaagtt cttcaatttc
    12061 tttctgaaac ataactatta ggaatagaag gtgatttatt tgttaaaaaa taccatacaa
    12121 tggctgaaaa accacctagg actgttcaaa acaatctttt tgatttctgt cattgtcatt
    12181 attgtcggtg aattgatgtc catcggtaaa actctatcta tccagcaatt aggagaaacg
    12241 tttgccacta tacctgtatg gaaaagcgga ctaatgctat tgattggtct tatctctgtg
    12301 ttacctatgg ttggttatga tattattttg aatcggatgc ttgaacaaaa gcagaatcct
    12361 cgttacctgt ttgagacaag ctggctgatc aatacgatca acaatattgc cggtttcggc
    12421 gggtttgtta gtgtggggct tcgctctgaa ctatatggac aaaagaaaga gagcagaaac
    12481 gtgatccaag cattatcgaa agttttcttg tttttgatgg ctggactttc tatttacagt
    12541 ctgctttctt ttttacttgt agtcttcacg ccagttgctc cttttttgaa gcaatattgg
    12601 atatggttga tcggcggcgg tctatatttt cctgtcgtct ttttatttac tactttcaaa
    12661 aagaaaggat tccttggtgg tacctcactt aaaactcaag gacagctatt gctagtttca
    12721 ttgttggaat ggaccggtgt cctgtttagt ttcctttctg ttggttattt aatggaaatc
    12781 cggattgatc tgtggcaaac aattccatta ttcattgctg cttcagtgat aggaatcgtg
    12841 tctatgattc caggagaaat tgggagtttc gatgtaatga tgattatcgg gttgtccgct
    12901 attggtgtgc caagagaaat agtcgttgtg tggatcttgc tctatcgcct gttctactac
    12961 atcattccgt ttttgattgg aatcgtcttt ttcttcaaaa atatcggttc cacttttgat
    13021 cagcgttatt ctggtatccc taaacaatta gcaacagaaa ttgcgcataa aatcgtggtt
    13081 gtcttattat acttttctgg aatcatgcta gtactttctg ccactatccc gcaagctttt
    13141 acagagtttc gctggcttca tagtttgaac ccattgaaat ttcattttat tatccaattt
    13201 cctagtatac tattgggatt tcttttaatc attatgggca gagggatcgc agcacgtgtg
    13261 aaacgagcat atttgcctac aatcttcttg attgttctag ctttacttta tgttttatta
    13321 agtgatttta gtttcacacc ggtggttttc ttgtctattc tgcttctgat cattcttgct
    13381 tctaaaaacg aattatttcg ggaacaattg atctattcat gggagtggcg tacgatcgat
    13441 ggaatcatta ttggtgcact gtccttgctt tacatcgtta ttggggtata caatctgccg
    13501 gatttccctc atcggcgtca ccattttatt tcctttttct tatttccttc tgaaaagatt
    13561 tggttttcgg gattgttggc catcatcgct gttagtttca tgatcgttct atttgttcat
    13621 ttccttcaag gagaaaagaa acagattggc gaagccttta atgaagaaaa ggccctgaag
    13681 atccttacaa cttatggagg aaactccgat agtcaattga tcttcttaaa agataagcgg
    13741 atgtttgcct atgaaaaaga cggtgaacca actgttcttc tccaattcgc ttgcttcaat
    13801 aataaatgta ttgtaatggg tgatccttct ggtaagaaag aagattttcc agaagcaatc
    13861 gaagctttta tcgaagaaac agatcgacta tgctatttac cagttttcta cgaaacaagc
    13921 gaagagattg ttatgatttt gcatgaattc ggctacgatt tcattaagat gggggaagaa
    13981 gcttatgtag acctgaatag ttttacaact tcagggaaaa aaatgaaggg cactcgtgct
    14041 gtcttgaatc ggatcgaacg agaaggattt acatttgatg tcttacaacc accattttct
    14101 actgagcaaa tgagtacttt caaaaatatc tctgataatt ggttgggttc acgaaaggaa
    14161 aaagggtttt ctttaggctt tttctctgaa gattatctac aaagagcgcc tattgcagtt
    14221 gtcaaaaata cccaagaaga aattgtcgct tttgcaacga tcatgcctac ttacacgaac
    14281 aataaagtag gaacgattga tctgatgcga tatgattcta aaaaagcacc ttctggcagt
    14341 atggatttct tgttcttgca tttgtttgcc tatatgaaag aagaacatat ccaatggttc
    14401 aatttaggga tggcccctct ttcaaatgtc ggaacttcca gaaagagttt tttacaggaa
    14461 aggattgcat acttggttta cgagtttggt tctcattttt attctttcca tggattaaga
    14521 gaatataaaa gcaagtacgc tacaaactgg gtctcacgtt atactctata ttctagagat
    14581 agttggattg catatgttat gattgctttg ctgattattg ataatgcgcc agttgagcag
    14641 acacaaggta gaataagtgg tttgaaaaaa tggatacgaa gaaagtatta aaaactgcat
    14701 accgatagat gaacactctc atgatgaata aaagagactg aagtaaaacg aaagttcatg
    14761 cttctacaat ctcttcttat acaattgaag aacaaagagc ggtccaaaca gaaatcctgt
    14821 ttggaccgct cttttatttt aaacattttt cattaccaaa atattcgagt cgcgtttctt
    14881 atgcttcgat ctctgtaacg atacctgaac ctactgtccg tccgccttca cgaattgaga
    14941 atgtcgttcc gttttcaaca gcgattggat ggatcaagtc aacatcaatc gtcacgttgt
    15001 caccaggcat gaccatttct gtatcttctg gcaatgtgat tgttcctgta acatcagttg
    15061 tgcggaagta gaactgtgga cgatagttgt tgaagaatgg agtatgacgt ccaccttctt
    15121 cttttgtcaa cacgtacact tctgctttga attttgtatg tggagtaatg gaacctggtt
    15181 tagcaagtac ttgtccacgt tcgatatcgt cacgttggat cccccgtaac aacacgccta
    15241 cgttatcccc agcttccccg taatctaacg ttttacggaa catttctaca cctgttacaa
    15301 ctgctttttg tgtttcaggt ttgatcccta cgatctctac ctcatcaccg acacgaacag
    15361 caccacgatc aatacgacct gaggcaacag ttcctcgacc tgtaattgag aagacgtctt
    15421 ccactggcaa tagtaatggt ttatctgtat cacgttctgg tgttgggata tattcatcta
    15481 ctgtatccat caattccata atagcagctt cagcatctgg atcgccttgc aatgctttta
    15541 atgcggaacc tttgatcaca ggagtgtcat cgcctgggaa accatattcg ctcaataact
    15601 cgcgaacttc catttctacc aaatcgatca attcttcatc atcgactaaa tcaactttgt
    15661 tcaagaaaac aatcaggtat tttacaccaa cttggcgtga caataaaatg tgttcacgtg
    15721 tttgaggcat cggaccgtca gttgcagaaa caactaagat tgcaccatcc atttgtgctg
    15781 cccctgtaat catgtttttc acatagtccg cgtgtcctgg tgcatcaata tgcgcgtagt
    15841 ggcgtttttc tgtttcatat tctacgtgtg cggtattgat cgtaatccca cgttcacgtt
    15901 cttctggagc tgcatcaata ctagcatagt cttgaggatt tgctaggcct tttttgccta
    15961 atactgtagt gatcgcagca gttaaagtag ttttcccatg gtcaacgtgt ccaatcgtac
    16021 caatgttaac gtgtggttta cttctgtcat aatgttcttt tgccataatt tacaaacctc
    16081 ctaaataata tagatcactg aaaaatttca gctctcacct ttttattata gtcttttttt
    16141 gactaaatca aaaagaatac gtccgactta ctttttgtaa gattcgaact aagcttatta
    16201 tacgtcaata ttggtcaaag tcaactaaga gcactcgtct gtcttatacg cgaaaaaagt
    16261 accttttata aataaaaaaa tgtgtttttt gagaaaaact tcaagatagt ccttaacttt
    16321 cttttgaagc aataagaaca aaatgaggaa aagtttaaaa ttcagctgtt gccaacaact
    16381 ttcaatagac gaaatcttct atttcgttaa taataaggaa agaggtgaag tacatgaaaa
    16441 aattaattgg cgtacttgtc gttttaggca tatttctcgg cggaagtttt gcagcttatc
    16501 attattttta cggcggcgaa gcttattata caaaaatcac gacgaatggc gaaatgtcag
    16561 ctacccaagc agataacgga gagaaattca cgacttatac ctacaagcaa gcggcttata
    16621 ataaagatgg tgaaaaaaaa caggtgacgc ttcgcgaaga aagagaacat ccattaaaga
    16681 tgaatgcgta tcttaaactc aaagtcaatc ctcgtaaagg tgtattaagc tgggaagaaa
    16741 taaaggcttc tgaagtgcct aaaaatgcgg cagaaaaaat cgataattag taaatggtct
    16801 taaaatttta ttagataaaa agaggctggg acagaagcgt ttaactccaa gaaataagaa
    16861 ggagttgctt ctgcttccgc cgtttatacg tgtttagagc gtgagacaaa agtgttcttt
    16921 acttttgtct cacgctcttt ttttgttata ttcgtctttt ccgatccttc tcttgacaca
    16981 attcatttaa ctgatcactg gtacttgaac acctaatatt ctagtcattt ttataaacga
    17041 ttcaatactt catccacatt tttgatcatc acggatatcg taccatccgg attattcaca
    17101 aattcaatca aatctgggtt gcgatataca tctactggta cgatcagttc gatcccgttt
    17161 gatagcttga atttttgttt tccaaatttt ttctcagaaa tttcttgtac ttcttttaca
    17221 ggaggtgctt ctgggacaaa tccttgctct tgtacttctt ctttaaaagc catttttgcc
    17281 gtgatgtttt ccttgaagac tttttccgca atcatctcat gatccaaccg acctttttct
    17341 tctatcgtat catagacagc ttctttcaaa tcagccatga tatcaaattc ttctgtctca
    17401 aatttttttc caagatgctt ggctactttt tggatcactt ttacattttc ttctaaagaa
    17461 ggtgcaggaa cactttcgat cactcttcca gaaaaataaa gctctttttt gccagaaaac
    17521 tcatattttt tttcaattaa ttcgtaggtc caatcagtca aattcacagc gaatgcttca
    17581 tctgcttttt gtgttttcgc ccctagaatt gctcgattca aaatcaagcg gttgtcgatc
    17641 cctgcttcgt ctgcttcaac aaagtgagtg taagcctcac gataattgac tttcaataaa
    17701 gataagtaca tcactgtatc taattcatac aagacaacaa atacatctgc actaggtgct
    17761 tcttcacttt cttggtagat atcgaaccac cgctgaacga ttgcttcact tgcttccaca
    17821 aaattttctt gagcgatttt aagtgattca acgatcatgc tttcttctgc tagttgtcca
    17881 gtttttgttt gagcagaaga gattttttgg atcttctttt gaagaaaatc acgaatatat
    17941 tcttttgtca gatccagctc tttttgtgaa aagactggag aaccagtttc tctgtcaata
    18001 atatgcagaa ttgctttttt taaatagata tccattttct cattcccttc tgtcttagtt
    18061 tacggaaaaa aaatcaaaaa taccagaatc aaaatcattt tcttttaaac aaaaatttca
    18121 agagtagaaa caaaatggat tctttttttc gaaaaatgcg ctacacttat agttgaacga
    18181 ataaaaaagg agctgaaagt atgaattcat taacaagcac ttatcgtcta tcaaatggtt
    18241 acgaaatccc ggtagtagga ttcggcacat ggcaaacacc tgatggggat gtcgctgttt
    18301 cctctgttaa agaagcgctg gctgcaggtt atcgccatat cgatactgcc caaggataca
    18361 agaatgaaga aagcgtcggc caagcgatta aggaaagtgg aattcctaga gaagagatat
    18421 tcttgacaac aaaattatgg aatgcaaacc acagctatga acttgtgatg tcttcttttg
    18481 aagaatcatt gaagaagctc caaacagatt atttagatct gtttttgatc cactggccaa
    18541 accctgttgc ttttcgtgat aattgggaac aagcaaatgc agatacatgg cgagcatttg
    18601 aagaactgta tgaagcaggt aaaatcaaag caatcggtgt gagcaatttc ttaccccacc
    18661 atctagatac actagcaaaa acagcaaaaa ttatgccgat ggtcaatcaa gttttccttg
    18721 caccaggcga attacagaca actgtagttg agtatgcaaa aaaacatgat atgatcctcg
    18781 aagcatatag tccattaggc actggtaaaa tcttcgatgt gcctgaaatg aaacaaatcg
    18841 ctgaagcaca tgacaagacg attgcacaag ttgctttgcg ttggtcgctg caacacgaat
    18901 tccttcctct tcctaagtca gtgacaccaa gcagaatcaa agaaaatact gaattatttg
    18961 attttgaatt aacagaagaa gaaatgaaac aaatcgatca gttagatggt gtagtcggca
    19021 aagcaaaaga cccagatact acccaatttt aagtcgaaag gtgattttat ggatccaatc
    19081 gaacgcttga attctctctc ggaagaagtt acccagactt ttcattccga ttttgtcttt
    19141 ttgatcgatg cagagaaaat ccaacatttt cctgcaagaa actggacaca tgatcaaatc
    19201 atcgaagaac tgaaaaaacg gttcgatcat tcgctaatgg tcaaaccctg gcacgaacat
    19261 gaagtcattt attcacctga actacctgtt tttgccttga taccgaaaaa atagaaagaa
    19321 tggaacggat tatgtatata tacataatcc gttccatttt ttatttttct ttcaaaaaac
    19381 gggaaaatac gcaaattttt ctgttgtaat ttgcgtatac gcaagttttt ttgcattact
    19441 ccttttcatt tggctgtttt tttcttataa tcgaaagggt atgagcgaag gagagaacat
    19501 atgaagaaaa aacagaaaca aaaattatta aatcaattcc gtccttcatt ggatggtgta
    19561 agaggacaat tattccgttc tctagaagaa gagagcttgc gacgttatag tttgccgtta
    19621 caagtcatgc ttgatcccaa aaaccgtcaa atcttaacga tcggattagc tgggaaaaca
    19681 aatgaagata cacggatcaa cgtgccaatc gatgacaact tcaatactgt attaaagaga
    19741 attcgttcag gagaaaacgg gattttcgaa cgattccgtg acaatcttct tattgaaatc
    19801 gtgtcttact ggaatgatca acgattgaaa aacgatgaac caactgcgca agctgttgcg
    19861 acaccagtta cagaagaaac agtcagtgtg ccagaagaaa aaacggaaaa accaaaagcg
    19921 gacacaactg aaaatacacg ctcaactgat acgaatgccg ctttaagttt tgctgatttt
    19981 tcaaaagccg ttgctgagta tcctaaattt tacacagaac aaaatgaaca tgaagtagtt
    20041 attaatgaaa aaaatgctga tgaacctcgt aaattagcaa ctatttctat gactactgaa
    20101 aatgagttcg ttatcgaaaa agcactcgaa cggaaataca aagtcaaatt gactttgatt
    20161 cctttgatcg aacaatttgc agcgacagcg attactg
//
LOCUS       DACDPS010000043        20133 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.043, whole genome shotgun sequence.
ACCESSION   DACDPS010000043 DACDPS010000000
VERSION     DACDPS010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.043"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..164)
                     /locus_tag="IWJ07_001681"
     CDS             complement(<1..164)
                     /locus_tag="IWJ07_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017826416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP9333262.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAEL"
     gene            493..1242
                     /locus_tag="IWJ07_001682"
     CDS             493..1242
                     /locus_tag="IWJ07_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAP9333263.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(1426..2703)
                     /locus_tag="IWJ07_001683"
     CDS             complement(1426..2703)
                     /locus_tag="IWJ07_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333264.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(2782..3426)
                     /gene="pcp"
                     /locus_tag="IWJ07_001684"
     CDS             complement(2782..3426)
                     /gene="pcp"
                     /locus_tag="IWJ07_001684"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAP9333265.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(3625..4569)
                     /locus_tag="IWJ07_001685"
     CDS             complement(3625..4569)
                     /locus_tag="IWJ07_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAP9333266.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(4571..5257)
                     /locus_tag="IWJ07_001686"
     CDS             complement(4571..5257)
                     /locus_tag="IWJ07_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAP9333267.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            5731..6051
                     /locus_tag="IWJ07_001687"
     CDS             5731..6051
                     /locus_tag="IWJ07_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAP9333268.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            6205..7110
                     /locus_tag="IWJ07_001688"
     CDS             6205..7110
                     /locus_tag="IWJ07_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9333269.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            7107..7880
                     /locus_tag="IWJ07_001689"
     CDS             7107..7880
                     /locus_tag="IWJ07_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9333270.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(8061..9485)
                     /locus_tag="IWJ07_001690"
     CDS             complement(8061..9485)
                     /locus_tag="IWJ07_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9333271.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            9696..10142
                     /gene="nrdI"
                     /locus_tag="IWJ07_001691"
     CDS             9696..10142
                     /gene="nrdI"
                     /locus_tag="IWJ07_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP9333272.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(10207..12189)
                     /locus_tag="IWJ07_001692"
     CDS             complement(10207..12189)
                     /locus_tag="IWJ07_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9333273.1"
                     /translation="MAKNELTLEELAKHIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(12203..13099)
                     /gene="murQ"
                     /locus_tag="IWJ07_001693"
     CDS             complement(12203..13099)
                     /gene="murQ"
                     /locus_tag="IWJ07_001693"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP9333274.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(13102..14163)
                     /locus_tag="IWJ07_001694"
     CDS             complement(13102..14163)
                     /locus_tag="IWJ07_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP9333275.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(14374..14901)
                     /locus_tag="IWJ07_001695"
     CDS             complement(14374..14901)
                     /locus_tag="IWJ07_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9333276.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(14968..16470)
                     /locus_tag="IWJ07_001696"
     CDS             complement(14968..16470)
                     /locus_tag="IWJ07_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP9333277.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(16728..17849)
                     /locus_tag="IWJ07_001697"
     CDS             complement(16728..17849)
                     /locus_tag="IWJ07_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP9333278.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            17975..18916
                     /locus_tag="IWJ07_001698"
     CDS             17975..18916
                     /locus_tag="IWJ07_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP9333279.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(19066..19962)
                     /locus_tag="IWJ07_001699"
     CDS             complement(19066..19962)
                     /locus_tag="IWJ07_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP9333280.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
BASE COUNT         5871 a         4240 c         3547 g         6475 t
ORIGIN      
        1 tccaattcag cacttcctag tttcagatct aatttatagt catctcgttc tttgcggtaa
       61 ttttctctga ttacttcaaa tcctaattta ttaacataaa aatcacgtga tttctgataa
      121 tctgaaacaa tgatggctat atgatggatc gttgtaaggt tcatatgagc actcctattc
      181 ttcgtttaag ctcggatcta tattaatctc cagttccatt gtaaatgtgt cagattgaaa
      241 aaaatagctg tttttccttt ttataagaaa aaaggcaaaa aaaacagaaa gtaggtcctc
      301 caagacactc ttcctgttcc caatataata aatgaaagta agaaattata ttttcaagaa
      361 aacaaacaga aaattatcat aacacaaatt atttataatt gaaagactag tttttttcaa
      421 gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta gtgtcttaag
      481 gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa aggctggccg
      541 caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac tcttgtcaat
      601 agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg tgtattgtgt
      661 attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt gtctgcgatt
      721 tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga acagattgct
      781 tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca taatatggct
      841 tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag tactttcctt
      901 gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg gccgtgggta
      961 gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt gcgttacaac
     1021 aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg gtatatttca
     1081 tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta cttgctgaat
     1141 gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga acgtatgcaa
     1201 aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg agcgtgggac
     1261 aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg aatcagaagc
     1321 aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt cgtgaatttc
     1381 ttcttatttc ttggagttaa acgcttctgt cccagcctct attttttata gtaattcctt
     1441 cagatacttt cttgcctcat cattgaaacg cagtgattct tctaacttct gataatattc
     1501 caacgcttgt ttctgagctt cagtaaaacg ataatgtttt cctttccatt tgtcgattgt
     1561 ctcttgcaca tttccagatt ggatatcttt cactactagt tgccattttt cttttgagac
     1621 tgtacggtct ttcttatcag cagattgtat agcttctcgt tctttacgca atacttttaa
     1681 aaaaccattt ctgactatcc gaggaatgct gtcgaagaac ccttctgtaa aaccatagcg
     1741 acaatattgc ttccaaataa atagatagat ttttggttgg atgaatcgtt ggtttttcgc
     1801 aaaatattcc atctgagcag tgacttctgg tgttcgttga cgttttgtct ttctcaacac
     1861 ggaaataaat gtttcaaaat aagcattagg atgtactggc acttcttcat atacgatgga
     1921 ttgccaatct ttacgtaaaa atgcgcgata gttgatcaag aattcttcta catcctttac
     1981 taattcttct gcaaagtcgt caaatagttc tttttttatc tgtcgctttt ttgactgccc
     2041 attgatgtgt tcacttattt tcttgatttc attttccaat gcttgataat ccgctatgta
     2101 aatcggcatt tctacttggc gaaattctgc gagccgctga gctaatttta tatccggtaa
     2161 atatggctgt ttcatccgac gattttggta caagacaaaa cagtattttt gccatagtcc
     2221 ttcaggaaaa tcgatattct tctgcttaag ccataataat tcgtctaagg ccagatcaac
     2281 ttttgtcatt ccctgatgga tcgaagctgc cactgtcgga gagacatgca ggtgatcggc
     2341 aaagtggttg atgcctagtt ttaggatctt tccggttttt ggcgatttaa cgatgtattg
     2401 cacttttaat tctctaccac actcacaata caatttttgc tggcttcgtt ttgttcgaaa
     2461 aaacggattg atcttgaagt cagaaaacac ccaatctgtt gcagctaaat aatgttcttc
     2521 cataaaacga gagctgatta gatatcggcg gtgcttatcg actagttccc gctgtttttt
     2581 cgttaatttt gcatagatct cccaacgttg actgcttgtg tattgtttag aaattttgat
     2641 gggatagctt ttgctgattt cttttggaaa ccattcttca aagacggatt gccattcttc
     2701 cacggctgtt tctttccttt ctaattacaa aaagctggct actaacataa cgttagcaac
     2761 cagccagtgt ttcttgctaa attagtgagt tgttccggca tttccggaaa tatcttcttt
     2821 attttctact gctgcttcaa ctgcataagt caaagaagaa gcaatgtctt ctaaagacat
     2881 agaaggcgta cccactggac gatcgatcac ttgatcgggt gagaatggca cgtggatgaa
     2941 cccacctcga attgccggga attgtgtttc gatcaaatga agcaaacgat acatgatatc
     3001 attgcagaca aatgttcctg ctgtataaga gatgtacgct ggtagaccat gatcttgtac
     3061 attttttgtc attgctttga ttggcaatga ggtaaagtaa gcggctggac cgttttcttc
     3121 taaagccgta tcgaatggct gatttccttc attatcaggg attcttgctt ctgctagatt
     3181 gattgcgaca cgttcgaacg agactgctga ccgcccacct gcttgtccga cacagatcac
     3241 gacatctggt tggtgctgtt ggatggcttc tttgactact tgactgcttt tttcgaatac
     3301 ggtcggtact tccactttga tgatttcagc accgctgatt tcatctggca ttttttttac
     3361 tgcttcatag gctggattca ctttgtcccc accaaatgga tcaaaacctg ttactagtac
     3421 tttcatctgt tgttcctccg tccgattgat ttaacatatg gttatctttt atcataccat
     3481 tttccttgaa cagcgtaaat aaaggaaaca atagtttaaa taacataaca gatcaaactt
     3541 aatatattct tataaaaaaa tagtgttcca ctacaatgtt agaagaggtt gcgacaatta
     3601 ttctgtcaca acccctctct tctattattt aaatagaatc atataagcaa tttggaaaac
     3661 tagcataaat aacgcaataa acaactggtt tttgatcact ccgtatttgt ctttcatctc
     3721 cagcatcgct acaggcacga tattgaagtt agcagccatc ggtgtcagca gggtcccaca
     3781 aaaaccgcaa gtcaatgcaa ccattcctat taacgctgga ttcgctccat gactaaatac
     3841 gaatggtgca ccgatcccaa ccgtcatcac ggtgatggcc gcaaatgcat tccccatgat
     3901 taccgtaaat atcaccatgc caagagcgta aatgatgatg ccgatgttca catttccttc
     3961 tggaacgatc gtacctactg cattggaaat cactgtccca actccagcgc ttgtaaatac
     4021 cgcacctaga gaagctaaaa gcattggcaa catgcttaac ggccctactg tgcccagcat
     4081 atctgctgca tcatctaaaa atgtacttgg tcgattatct cgagaaaaga acatcaagat
     4141 tagcatggct gcaaaaacac cgacgcccac gccgactaaa gcacctaaat tggtaaatag
     4201 agcaaacaag atcgcaaaga caccgatact caaagctgga ataaaaattt tcattcctag
     4261 tttgtctgac attttttcca tatattcttt tgatggaagc cggctttctc ctttgctgac
     4321 tttcttagca attgctggta tggtcatcgc aaagatcaag ccaccgttca cgattgccgg
     4381 aagccatctt cctcctgcaa taactacccc taagaccgtc cagaaaaagg ctgacccata
     4441 acgatgagga ttggttttat ctgttaaatt tcttactcca gtgtaaatca atacgagtcc
     4501 catcacgata taaaggatct ccagtaattt atcgcctaat aaaatatcag gatttgtaaa
     4561 aaagtccatc ttattgcccc tttcctggat aatatttctt cgctaatttc ttttctttaa
     4621 gatagaagta gacacttgcc acaactaaag ccgtgatagc cactggaact tccactaaag
     4681 caagctgtcc caatgttacg tcatatccta gatctttcag tgttgattgg actagcaacg
     4741 cccctgctcc tccgatgaat agtacttggc caaagaacca gcagacattt tccatagctg
     4801 aagccatacc ttttagttct tcttcatgct taggatggac ttctaaccct tttgattcga
     4861 tggtccccaa tgccatcggt aaaatgattg gacgaacaaa tccagcaact ccaccaaaac
     4921 tcacgttaaa agccgcaaag atcattcgaa agatcccata aatatctaca atcataccag
     4981 cagatacttt cttgaatcgc ttgatcaagg aagccgctga ttctttcaga ccatttcttt
     5041 caagcgttcc tgttgccaac ataatgatga taaaaatcgc catcccgcga ttgtttacaa
     5101 aacttgtacc taaaagttct aacattcctt gaatgccaag accgccagtc aaagctgtaa
     5161 caactaaagc aaccattacg atcaatatcg agtctaattt aaatgcaaac ccgagaataa
     5221 caatcaagac gcctaaaagg ctaagataat tttccatata ttttcctcca taaaatgaca
     5281 atattttttt aattataaag caaaacacat aaaaaatata gttgttttct cacgaaatag
     5341 acactatttt taaatatttt tcgttatttt tttatcacaa aaattatctt acttattgta
     5401 aggtaaagct ttttttaatt tattgcccga taatatatag aaataaggtc tagttactgt
     5461 tttttttaaa tgattaataa ataacaactt ctttctatga aattgcaaag cattcatctt
     5521 cttttccgct aacgatatat ccgtcagtgc ttggtacact ttactaaaaa gtatgagatt
     5581 gccgatatct ggataggtgt gctatactac agaaaaagat atacaatctg ccaccgggta
     5641 ggataaagag gatgatcatt ccattaggtc tttgaaggga tgattttcct ctttttgtgt
     5701 atttttttat agttaaggag gaatgtaagt atggcatgga tggaattaat catagcagga
     5761 attttggaag tattctggtc aacgatgatg aaatggagtg atggtttttc taaaatcaat
     5821 tattcttttt acacagtgat tggcatgatt gctagttttt actttttgtc aaaggcaatc
     5881 aaaagtttgc cgatgagtct ctcttatcct atctggacag ggattggcgc agttggttcg
     5941 gtcataattg gtgtggtctt gtttcatgat aagctgaatc tttccacttg gttctttgtg
     6001 gggatgctac tcatcagtat cattggcatc aagattacta gcggccacta gacacttgga
     6061 ataagtactg atcagaaaaa ataagccttt ttatttaata ttcaattttt ttatgaaaaa
     6121 ataagaaata agtccaatga cctagcgact tctccactca tcaggtgtat gcttgtacta
     6181 tctatagaaa aggagtgaat atttttgaaa agtctttcgg tccaagaatt atccaagaac
     6241 tttggtcaca aacaagccct cagcgatgtc agttttagct tatcccctgg caaaatcgtt
     6301 ggattagtag gtcctaatgg cgctggaaaa acaacgatca tgaaagctat tttaggactg
     6361 atccattact cagatggaac aatctgtatt ggtgaaaatg agatttctcc ctctagtcat
     6421 aaagggttgg aacaagtagg agcattgatc gaatacccgg gtatctatcc attcttaaca
     6481 ggctatgatc atctgaagtt attctctgaa accaacgatg tatctgctat cgacactatc
     6541 gtgaatcagc tcaagatgga aaagtacatt cacaaaaaag cgaaatccta ctcgttggga
     6601 atgaaacaaa aattgggaat tgctcttgcc ttactgaacc atccgtctat cgttatttta
     6661 gatgagccga tgaacggttt ggatccacaa gcaacaagag atgtgcgaaa aatcattacc
     6721 gacttggcta ctcagggcac ctctttcttg atctccagtc acttactcag cgagcttgaa
     6781 aaaatggtcg atgagctgat cctgatcaat caaggaaaga tcatcaaaca atgtacaatg
     6841 gctgaattga accaaacagc gcaagatttc atcgttatct ctacaacaaa tgatctagct
     6901 gctcgaacga tcttgacaga agctggatat actttggaag atcctgataa aatcaagctt
     6961 ttaaaagaaa acgaagaatt attaggaacc gtgatccagc tattgtctgc acaaaatatc
     7021 catgtgacag acgtccagca tctgacgaat gatttggaat cttctctatt ggaaattctg
     7081 gattcagtaa agggggaaga agaaaaatga cattgcttgt aaaacaagaa ttatttaaat
     7141 tgatcaagaa aaagtcaact gctgttttat ctgttctatt agttgttttg ctcataggaa
     7201 cagcacttct agccaaaaaa tacacgacga tcattgatcc agttgaaatg actgctcagc
     7261 tttttagtgc cacatcttgg attgtcttta tcatgattgc agctgcaagc acgatcattt
     7321 cgatggaagc acaatatggt acattgaaaa atctccttta tcgaaagtat tctcgaggag
     7381 aaatattagt aagcaaatgg atcacattag ttatttattc tgtttattta taccttttag
     7441 ctattattgt cactgtgttg atgaaactga ttcttttccc ttcgatttcc ttcacggaga
     7501 aggtttctac tggtcaaaca ctgatccaaa gcttgttcac ttatactttg ggtagttata
     7561 tcggtttatg gctgatttta agtttagtgt tgatgattgc ttgctttatc aatagttccg
     7621 gcgcatccat ctcagcagga atcgtctttt attttgcttc gtcaatcata tctggtatcc
     7681 ttattgcact catccaaaaa tgggagtgga tcaaatggaa tccgatcagt atgttgaatc
     7741 tgcaaaacca aattggaaac gaagagatca tgaagcaatt gactcatctt tctactaatc
     7801 aaatgctatt cggtaattta gcttacatcg ttttgttctt agcattagga tatctcgtat
     7861 ttaagcgaaa aaatgtatga tgactcatca aaaaagaggc tgtgaaagag acgttcagcc
     7921 ttattgccga ttggctgtct cttaaacacc gtttattctg aaaaaggagc tgtgacaaca
     7981 tttgtgtcac agctcctttc ctagtatgtt tttgaataaa cgacaggctc acaacctctc
     8041 tttatacttc ttttatactt ctatacgtga agcatcccag tggcaaataa aaccattgca
     8101 atcactccga ccatcgtcac acctataagt aaaatcttat ctaagtggat atgttgtaca
     8161 ggctcgtttt gctccttacg tgctttgagg taaaaataaa tgcctggcaa gaaaagaaca
     8221 agaacaagca ttacttcatt aaaggctacg actagcatag cccatacttg aaaggcactt
     8281 gctaaaagcc ctactaaaaa ttggacccat tctttccgct gataagaaag tttcatttgg
     8341 tacaatccta agaaaagcca cgtgaagaaa atagctgctg taccaagtgt gtaagcaaag
     8401 ttataagcac tgtcagtgaa taataatgaa agtaaaaaga cattcgtaca taacgtcgtc
     8461 aatacgatag cataagttgg tgccccattt ttgttgagtt ttccccatgc tttaggcaaa
     8521 agtccgtctt cagccatgtt tcccattgtt tcaatcggta agatcgtcca tgagagccaa
     8581 acaccaacta aagaaatgat caaaccgccg ttgatcacga ctgctcccca tttaccaacc
     8641 atcatttcaa acacatagcc catagcaggc tgcgccttga tcgtttgcag ctgattcatc
     8701 gtcattgtcc catatggaag catggaaacc aagacgtaaa taataatcaa gccgaatatc
     8761 cctagaatcg ttgctttccc aacatctgat tgtttttttg ctcgtgaacc tacgacagag
     8821 gctccttcaa taccaacaaa tacccaaagc atcaccatga tgcaactcaa tacttgactg
     8881 gaaaacggga tcggctgtcc attcgcattc aatataatgt cgctagaaaa gttatcaatg
     8941 aattgttgga tcgaaaacgt gctgatagct actacaataa ataaaaataa tggcactaat
     9001 ttacagaatg tgacgatagc attgacgaaa ctagcatttt cgactccccg attcactagc
     9061 catgcatatc cccataaaaa gactgtactc atcacgatcg atgccagatt ttgcccacct
     9121 tcaaatactg ggaagaaata tcctaatgcg ctcatcaaca atgttgcaaa agctacgttg
     9181 cctaaccagc atgtgagcca gtaaccccaa ccgctgataa aaccagcgaa acggccaaaa
     9241 ccttcttctg cgtaactaaa gatcccgcct tttaaatctg gacgtttttt attgatatta
     9301 ttgatcgaca aggagagcat caacattgct gttcctacga gtacccaact gaaaatagct
     9361 gggccgccag ccgatccgga gatgtccgtc ataataccaa atacgccacc tccaatcgct
     9421 gaaccaatta ctaaggcagc aagtggaatt aaaccaattc cttttttctg ttgctctgta
     9481 tccatgtaag catcctccaa aataaaaccg cttttatcaa aaagtataac aaaccttttt
     9541 tatttttact taattaataa aaactttaat agaaatataa ttctaccggc ttttttctca
     9601 ttatacaagc aaagcgccta gtccctttcc aaattacacc accaaaaaac ttaaaaaaga
     9661 tgctatacta aaaggaaaga attggaggat gatccatgaa cattttatat atatcgattt
     9721 caggaaatac gcgtgccttt gccaaacatc tagcagaata tgctgaaaaa atgcatgcag
     9781 aagatcctgt caatcctgaa gttactttaa aagaaatcca tgaaaatagt gattttacga
     9841 aagaaacaga accatttttt actttcgttc ctacttattt agacggcgga aatggtttag
     9901 ataacggtga tacagaaatc ttgaccgaaa cgatgcgtga atatctggaa catgaagaca
     9961 atcataaact ttgtcttggt gtagtcggta gcggaaataa aaacttcaat aatcagtatt
    10021 gtctaacagc caaacaatat gcacaaacat ttggctttcc tttcttggca gattatgaat
    10081 tacggggaac accatctgat gtgacacgaa tttatcagat tttagcagaa aacaataaat
    10141 aataaaaaag aagaagctga aaaatagatt tcagtttctt ctttcgtttc tattgtttct
    10201 atcatattaa gctttgacat ccatcacagg tgcttttgct tttgctttac ctgttttttc
    10261 caagacaaag ttttttacac gatccatatt ggttaccaag acgatcatgg atgtttcttt
    10321 gcctgctgct ttgatcgctt ctaagtcagc ttctgctaca agcgtatcag gtgtcacttg
    10381 ctgcccttct gttactttca catcaaatgg cgtaccgcct aagtcaactg tgttgattcc
    10441 catatgaagc aagatttcta aaccatttgg cgttgtgatc ccaatagcgt gtttcgttgg
    10501 gaagacagag ctgactaccc cagtaactgg agaatagata tgtccgtttt ctggttctac
    10561 aaagtagcct tcacccatca tcttttgtgc aaaaactgga tcacttgctt tatctaaaga
    10621 aacaactgtt ccgtcagcaa ctgatgagag gatagtcaca ctttcagctg aagtttgagg
    10681 tactactttt tcaggcactt catttactgt ggctgtttca gtcgctaatt cttctttttc
    10741 taaacggtct ttaggaatac cgaagaagaa tgtagcaacg aagccaccag cataagctgc
    10801 caataatcca aggatgtagc ctaaccattt accgtcagag atcaatggga tcaaagcggc
    10861 tcctgaaggt ccaatagcga tagccccgac atttccgatc attccgatca ctgccccgcc
    10921 gatacctcca ccgatacaag cagtaataaa tgggcgtcct agaggtaatg ttactccgta
    10981 aatcaatggt tcgccgattc ctaggatccc gactggtaaa gctcctttga tcatttctgt
    11041 taattcttta tcttttttgc agcgaatcca taatgccaat gctgcaccca cttgtcctgc
    11101 accagccatc gcaagaattg gaagcaatag cgtacgacca gtttcatcaa tcatttgcac
    11161 atggattgga gtcaaaattt gatgcagtcc gaacataacc atcggcaaga ataatgtgcc
    11221 tagaacgaag ccggcaaatg ctccaccaac gccaagtacc cagttgatcc caccgaccat
    11281 gccatcagaa atgaaacctg ctaaaggcat gatgaagaaa atttcaatca gaccgatggc
    11341 aatcaatgaa agagttggtg taacgatgat atctaccgca tcaggtacta ctttatgcag
    11401 ttttttctca acgattgaca atagccaaac agcgaagatg acacctagga tcccaccttg
    11461 tccagctgca agagcttcac cagtaaataa gtttttcagt ggtgcttccg gattcatccc
    11521 tgtcagcatc acaacggccc cgataactcc accaagagtt ggtgttgcac caaaaacttg
    11581 tgcagcattg atacctgtat agataactag gtagctaaac atcccgtttt tcaagatgtt
    11641 catcacatcg atatattgtt gccatgttgc tgcatcaagt gttccggctg tgactaagtt
    11701 tgctaaaact gcggcaatcc ctgccacgat cccagtaccg acaaatgcag gaatcattgg
    11761 cacaaagata ttagaaatgt cttttaatac cgatttgatt ttagatggtt tttgtttcga
    11821 tttttgtgcc gctttcattt cttgtgcccg ttcgtttaca cggtcttttc cagatgctga
    11881 tgaatttgct gtagctgtac ttacagaaga aagtttttct cctaatttca cgcctgccaa
    11941 atcaaccatt tgctgtgcta ctttgttcac tttcccaggt ccgatgatga cttgaagctg
    12001 ttcatcttct actacaccaa gcactcctgg aatcgctttt aattcagaga catttacaag
    12061 atcatcattg atgatcttca tacgcacacg agtcatgcag tggacaatgc tttctacatt
    12121 gcccatgccg cctgcccctt tataaatgtg ttttgccagt tcttccaaag tcagttcgtt
    12181 ttttgccatg gtcttttccc cctcataagg tcccttttac aaatccgttt gcttcagcta
    12241 attttgcttg agcttcctct ttatcactat ttgtcaaaat catgacgata gccagcttaa
    12301 cattttgatc tgcttcttca aagtactttt cagctgtttc gtaatcagct tctgtcgctt
    12361 gcatgatgat tcgttttgag cgttcgatta gtttttcatt tgttggtttg acatcgacca
    12421 tcaagttatt gaatacttta cctataccaa tcatactgat agtagaaatc atattcaaga
    12481 taagtttttg tgctgttcct gacttcaatc gagtagagcc tgtcaaaaat tctggacctg
    12541 catcgacttc gattgggaaa tccgcgtgtt tactgatttc ggcttgttta ttgcaagaaa
    12601 tcgttcctgt gacagcaccg atcgattttg cgtaatctaa tccaccgatc acatatggtg
    12661 tacgaccgct agctgcgata ccaataacta tatcattttt tgtcaaattc agcttgatta
    12721 gatcttcttg ccctagctca taagaatctt cggccccttc gacagctaca gtcattgctt
    12781 cttctccacc agcaattaga cctatgacca tttccggttc tacaccgaat gtcggtacgc
    12841 attcagcagc atctaacaca cctagtcttc cacttgttcc ggctcccata taaatcagac
    12901 gtccaccttt tttaaaggac tcaatcgtgt tagcaatcac tggttcgata cttgtcagtt
    12961 ctttttcaac cgcagcggcc actttctgat cttctttgtt cattagttga acggcttgag
    13021 aaatactcat ctcatctaat ccaaaagttt cttggtttct gcgttccgtt gttaggttct
    13081 ctaaattgat tttgctcatt tttatcttcc ttcttttctt ataaattgat agttcatacc
    13141 agctttgatt tggttgatca atggtagctc ttctttgata actttagcta ctacattcac
    13201 tttttcatcc gctggcaaat catatttggt taattggatc tcacccatat agcgcaaata
    13261 cttttcgtta tctaaagtaa tagacccttt catacgcgta gctgtatttc ttgctgggat
    13321 aggcggaatc tctcgaaaac gtgcatctac gcttcgtatg acatctcttg catcgtcttg
    13381 ccgattcgta tgcttcccta atatatattc gtaaaattct tcatctacag gttccgtatg
    13441 gagcaagact gtctgttctt tttggaaaga agcaaattgc tcctgaacgt tttctgacag
    13501 acctgcatct ccgatgtaaa caatatccgt gttacttgct aatagatcta gagcgcctgc
    13561 aagaggatgg atccctcgat gttcttccaa agttggcaat ccttgatata gtgggcctct
    13621 caactctgta tctcctggta caaaaccttg gacaaggaga ccttgaattt tgagccattg
    13681 attttttttc tgataccatt ctttgtccag ccctgtttcg ggacgtggat aataattatg
    13741 ccaagcttca atctgtgaga aatcagcttc tgcttctttc aattcatcga tatctttagg
    13801 cgtgatcgtg ctagcattca aactgatttt caattgatgt gaccagtcgg caatctgctg
    13861 attggaaata tgataatcca ttcttaaacc tgttacacca attgctttca aaggcgttag
    13921 atcttctaat gaaaaacccg ctcgttccaa tgcctctcct gaaatatcga ccatcaattc
    13981 tagtttattc tttttcgctt cgcttcctag ttcgatcaat cgcttttgat atgccgaaac
    14041 atcatcttct ggtatatgca tggaagtgaa aataccgaca aagccattgg atgccatttg
    14101 gcggatatac tgtttcttct cttctgacaa agaatcgttc ataaatattg aaaagccaaa
    14161 catattatcc ctctttttct gtatccgtct tcatttctat gactagagta tatatctaaa
    14221 gaaacaaaat ttcaatactt ttattaaaat aattaaattt catttctaaa ggttgagttt
    14281 atattatcaa aagaattgtc ataacctaaa aaaggcactg ctaagcaaaa gcagtgcctt
    14341 gaaatatttt atttaaataa cacaaaaaca taattactca taagaaacca attctgtatt
    14401 atctaccatg aatgtaaaga acccctcatt aggcatcgga tgaaaataag cattgatcac
    14461 tcttatcaag gcattatggc aaactaacag atagacattt tcttcgtctt cgatgcattc
    14521 tttcaataaa ggaacgatcc gtgcatacac atctaagact gactccccat ttgggaatcg
    14581 tacagcgaac gctaaacgtg ctttttgaaa gttctcatct ttgcttggca gtccatcata
    14641 atccccaaaa tccatttcta ccaatcgttc atcaacggtc agcggcaagc tgagacgatc
    14701 agcaactgct tgtgctgtat ctcttgctcg ttgaagaggg gaatggatga ttttcgtgat
    14761 tggaacaggc aaatctacta ccttttctgc tagtttctgt gcctgcatct gtcctttttc
    14821 agtcagcggc acatctgccc gtccgcaaac taatccttgt gcgttccaag ttgtttctcc
    14881 gtgtctcgtt acataaagca tgtgattctc tccttcgttg gcaaagttcc ccttaaccat
    14941 accttgttgt tcttgagatt acaagggtta gattctctca agagaaataa tctcagtaga
    15001 tactttatcc aggaaggttt gatcatgttc cactagtaac atggcaggtt tcacgttcaa
    15061 gatcagttgt tctaattgtt cttgattgaa gacatccaaa taattcaatg gttcatccca
    15121 tgtatatagt tcagctggct gtgataaaga tttagccaat tccacttttt tccgttggcc
    15181 catactcatc tgctcgatct tgttatgaaa aacatctctt tccatcccaa gctttcggag
    15241 gttgttcaaa aatgcttggt agtctacttg gtttttctct gcaaattccg ccaacgttcc
    15301 tcgattgtct tcataatttt ggcttgcata actaatgctc agatgttttg gctggtattt
    15361 ttctcctata accttgccgt tgaatgcccc cagaagataa tggataatgg atgactttcc
    15421 tgcaccgtta ggacctgaaa tcgccacccg ctgatgaggc tcgattgtaa aatgaattgg
    15481 ctcgaataac agattttcat accctaattg aagatcttct acgcttaaaa gtcgcttatg
    15541 gtgagacgct tggctattca tcgtcaaaga atcgatatat tcgatatctt ttaatagttt
    15601 ttctttctcg ctgatctggg tctccatccg attcacgatt gctttggatc gtttcatcgt
    15661 ccgtgcagca tcagcaccca ctgctccttt attcactcgt ctagattcag tatcgatgaa
    15721 tccgacttgt ttctttgttt tatctccttc tcgggaacga gaccattcgg ctttttcagc
    15781 tgctgttttc tttagcctac tgacttcttt tttcaatttt tcattttgag ccatttcaaa
    15841 ttcatcacga agttttttct gttcttcata gatagagaaa ttcccttgat aaagttccag
    15901 ttgacttttt tcgattgcta aaacatggtc cactacttca tcgataaatc cccggtcatg
    15961 gctgaccacg atgaagcctt gttttttctt tttcaaataa gccgctactt gttttctacc
    16021 agagatatcc aaatgattcg ttggttcatc gattaacggg aaatgagtgt catccacaaa
    16081 taaaagtgcc agtaggactt ttgtcttctc tccccccgat agtgtgctga attctctcca
    16141 taagatttca ggatctgttt gcatcaattg gagctctctt tcgatttccc atatctcaaa
    16201 atccgtaata tcatttaaca cgtaataggt taaacgttct ttatcttttg tctgttgcgg
    16261 gaaataggca aattcttgct gatggattac ttgcccttga taaggtagtt tgttttgtag
    16321 aatattcagt aaagttgtct ttcctcgacc gtttcgtccg ataagtccta gtttccattg
    16381 tgtgtcaaaa tttagatttg cttgttcaaa taataatgtg ccttggctgt cgtagccgaa
    16441 tgtcagattt tttatttcga ttttagacat tttccatcac ctcgtaaaaa aagaccagtc
    16501 ctattttcag aactggctaa agaggatgca agggttggat acacacaaaa agacccatac
    16561 atcgaaaaaa ccaagccaat cccgaaaatc tgcacactgc ttccttgtct ccacaaggta
    16621 cagtctgaat gcagctttca tcaatcgact taatttttca atgcacggat ccctcacttt
    16681 gtcctatatt tgaaattacg atagcataaa gaaaatgatt ttgtcaacta cttcgggagg
    16741 atggtgaaaa atctgctaag tgtctgtcct aagatttttg ttttttctaa tgtttgcctg
    16801 tgttccattg cttcttctaa acctattgga cccaaatgaa tacttaagac aattgggaga
    16861 aaatcagaaa gtccttctag ctcttggtct ctacttccgc aaatagcgat tattggtttt
    16921 cgatattttt ttgcaagcac tgctacaccg tatggaactt ttccgtggat cgtttgctga
    16981 tcgatccgcc cttctcctgt aatgatgaca tctgcttgtt cgatcttttt ttccaatcca
    17041 gtgatttggc tgactaaatc gaaaccagaa accatcttcg ctcccaacaa aaaaagggct
    17101 ccaccaatac cgcctgctgc tccacttcct ggaatatcat ctaaatccac ctcccagaaa
    17161 tcttttattt gctgagcaat ctttcctgct tgtttatcta agaaagctag cgtcgtatct
    17221 attcctcctt tttgtcttcc aaattgccaa gcagctcctt gttgacctgt atatggattg
    17281 gtaacatctg cagcgataat caattttact ttagaaacaa gaacgtttct tcgatcaaaa
    17341 tgaagttttt tagtagataa taaaggattt ccttctgtgt acccttctaa agaagcaccc
    17401 aaggattgga gtaatcctag accaccatcg ctacacccgc ttcctcctaa agtgacaata
    17461 atttgttcta cttcttgaag cagtgcatct ttaataagtt ctcctaaggc ataactggaa
    17521 gcaagtcgga tagtttgatc cgttggttta tcaagtagat ctaaccccaa tgcttgtgca
    17581 gattcgatga ctgcggtgtt ttttccttgc caagttgtca ataaatagct gatttttcgc
    17641 tgacggaata agagatccat cccatcaatg gtacgccatg ttcccctgag cgcgtaatag
    17701 attgcttctg cagtattttc tccgccatct gcaatcggca cattgatttt tcttccttta
    17761 cgatcttccc agccatttag aactgcctga tttagttcaa atgaagaagc acttccttta
    17821 aatgaatcaa ttgccgccaa aatatccatc tgtatccctc actttcttct taagtaagac
    17881 actttttttc atttttttca aacaaaacaa tgctattttc ttccattttt ctatatacta
    17941 aaagggaact cattgaataa aggagagaaa aaacatgaaa atcgcaatag ctggggccgg
    18001 agcaatgggc agccgaatcg gattgatgct acatcaaagc ggaaatgagg tcctcttgat
    18061 cgatcgttgg cctgctcaca ttgaagccat tcgtacaaat gggttgattg ctgactttaa
    18121 cggtaaagaa gtggtcgcaa aactgccaat ctattcacca gaagaaatca tcgaaagcaa
    18181 cgaacacgtg gacttgatcg tagcattaac aaaagccaat caattagatg atatgttttg
    18241 ttcgatccaa tccattatta ctgacaatac ttatgttttg tgtttactaa atggattagg
    18301 tcatgaagat gtactggaaa aatatgtgcc aaagaaaaat atcttatttg ggatcacgat
    18361 gtggacagct ggtctagctg gtcccggaaa agtcacattg ttaggtgatg gagaaatcga
    18421 actagagaac ttggaaccag aaggcgaagc atttacaaaa aaagttgtag aagtctttca
    18481 agaagcaaat ttaaatccga tctatagcca taacgttcgt tattccattt ggcgaaaagc
    18541 ttgtgtaaat ggaacattga atggtttatg cacgattttg gattgtaata ttgctgaact
    18601 aggcgcgcaa aaagcggcag agagcatggt tcgaacaatc gtatctgaat ttgcttctat
    18661 cgcagcaaaa gaaggaatca tactagatca agaagaagtg taccaacata ttgcttctac
    18721 ttatgatcct gacaacatcg ggttgcatta cccatctatg taccaagacc tgatcaaaaa
    18781 ccatcgtttg actgaaatcg actacatcaa tggtgccatt tggcgaaaag gacaaaaata
    18841 tgatattgct acaccatatt gtgccttttt gacccaatta gtccatgcaa aagaaggaat
    18901 ccttggcgca gaataaaagg ctctctgatg agaatcagct gaatcagcag ctggttctct
    18961 ttttttaagc ccaataaaag atcaaaaaga aaacacaact ctcatgatgc cataagaatt
    19021 gtgcttgttt gtttcagtac ataatctctt caaaatgtag gttttttatt tgttttcaac
    19081 agaggagagg tcatattcgt attgctcata aatagtggaa tcgttagtga taaatcgttt
    19141 tgcccaattt tctgcatcat aaaattccaa tagctctgat aggattggct taaatggtgt
    19201 atgcagaaat tccggtaatt catctaaatc acgttctcga agcaaaaaaa tattttcagg
    19261 acgataaaaa tgataatgag caatcgcttg attatccgtc actagtttct tttgaaaata
    19321 catggattca aatgtccgca aagtcactcc ttgctgaccc tcccgcaaca gttccagaat
    19381 cccgcgcgat tcccctgttt ttttcaggta ctcttcataa gtaagatatc caggttgttc
    19441 attccctcga tgtggaagaa tgaaatagtt ggtcgtaaca tctcttttta taaattcctg
    19501 cttatagctt tctgctcgtt cttttctccc attattcgtt gcaccaaaaa atagatccgt
    19561 ctttggttcg ctagaagaaa aattcatatg tttcgaataa aatgaactat tatgtttcag
    19621 cccaagttct tttgcttcca aaaaatcaaa atgataaatc tcatcgattg ccggttcatc
    19681 ttgtgcctgc ttcagcatcg cctcgtgttc tgctttgaag tgattccaaa aaaataacac
    19741 aatttttgca ttggtaaatg ggcgcacgcg tcgagccatc tccatatcaa atccataatc
    19801 taaaaaaacg atcagctgat aatttgccaa ctgttgtcta gctgatttaa tgaattccga
    19861 ctgtagtaaa tcttttccgg accaaacatc taaagagcgg atggaaggca aaaaataaag
    19921 tgatttattt tttgcaacta gcagtacgtc atttttgttc atcttttccg cctcactttc
    19981 tctgtcactt tttcctatga tagcctgttt aaatagtaaa aagccagtaa aacgtttcct
    20041 atatttgtat gaaaatttaa catttgtatg aaaatatgat gaaataaatc attcttctat
    20101 taaaattcgc ctaaaagttt tctgaatagt tgt
//
LOCUS       DACDPS010000044        19921 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.044, whole genome shotgun sequence.
ACCESSION   DACDPS010000044 DACDPS010000000
VERSION     DACDPS010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.044"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            11..1438
                     /locus_tag="IWJ07_001700"
     CDS             11..1438
                     /locus_tag="IWJ07_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP9333281.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1530..1895)
                     /locus_tag="IWJ07_001701"
     CDS             complement(1530..1895)
                     /locus_tag="IWJ07_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP9333282.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(1892..2257)
                     /locus_tag="IWJ07_001702"
     CDS             complement(1892..2257)
                     /locus_tag="IWJ07_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP9333283.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2449..2697)
                     /locus_tag="IWJ07_001703"
     CDS             complement(2449..2697)
                     /locus_tag="IWJ07_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333284.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2696..2824
                     /locus_tag="IWJ07_001704"
     CDS             2696..2824
                     /locus_tag="IWJ07_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAP9333285.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2881..4575
                     /locus_tag="IWJ07_001705"
     CDS             2881..4575
                     /locus_tag="IWJ07_001705"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAP9333286.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4642..4848
                     /locus_tag="IWJ07_001706"
     CDS             4642..4848
                     /locus_tag="IWJ07_001706"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333287.1"
                     /translation="MFLIWLDLLPQMQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFL
                     TWLDLLPQMQAFEHRLPRGYEKGV"
     gene            5226..6359
                     /locus_tag="IWJ07_001707"
     CDS             5226..6359
                     /locus_tag="IWJ07_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAP9333288.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6390..6710
                     /locus_tag="IWJ07_001708"
     CDS             6390..6710
                     /locus_tag="IWJ07_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333289.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6739..7203)
                     /locus_tag="IWJ07_001709"
     CDS             complement(6739..7203)
                     /locus_tag="IWJ07_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9333290.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7335..8588)
                     /locus_tag="IWJ07_001710"
     CDS             complement(7335..8588)
                     /locus_tag="IWJ07_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9333291.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8617..8934)
                     /locus_tag="IWJ07_001711"
     CDS             complement(8617..8934)
                     /locus_tag="IWJ07_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP9333292.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(8960..9280)
                     /locus_tag="IWJ07_001712"
     CDS             complement(8960..9280)
                     /locus_tag="IWJ07_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9333293.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9744..11159)
                     /locus_tag="IWJ07_001713"
     CDS             complement(9744..11159)
                     /locus_tag="IWJ07_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9333294.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11176..12492)
                     /locus_tag="IWJ07_001714"
     CDS             complement(11176..12492)
                     /locus_tag="IWJ07_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9333295.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12642..13340)
                     /locus_tag="IWJ07_001715"
     CDS             complement(12642..13340)
                     /locus_tag="IWJ07_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAP9333296.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13629..14618)
                     /locus_tag="IWJ07_001716"
     CDS             complement(13629..14618)
                     /locus_tag="IWJ07_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAP9333297.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14698..15531)
                     /gene="msrA"
                     /locus_tag="IWJ07_001717"
     CDS             complement(14698..15531)
                     /gene="msrA"
                     /locus_tag="IWJ07_001717"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP9333298.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15706..16974)
                     /locus_tag="IWJ07_001718"
                     /pseudo
     CDS             complement(15706..16974)
                     /locus_tag="IWJ07_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            17152..17448
                     /locus_tag="IWJ07_001719"
     CDS             17152..17448
                     /locus_tag="IWJ07_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333299.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            17455..19407
                     /locus_tag="IWJ07_001720"
     CDS             17455..19407
                     /locus_tag="IWJ07_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAP9333300.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(19479..>19921)
                     /gene="dfr_DGK"
                     /locus_tag="IWJ07_001721"
     CDS             complement(19479..>19921)
                     /gene="dfr_DGK"
                     /locus_tag="IWJ07_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DfrD/DfrG/DfrK family trimethoprim-resistant
                     dihydrofolate reductase"
                     /protein_id="HAP9333301.1"
                     /translation="DIPWRIPKDWEYVKNTTKGHPIILGRKNLESIGRALPDRRNIIL
                     TRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIYNLFFPYVEKMYITKIHHEF
                     EGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYERKNLLS"
BASE COUNT         6367 a         3870 c         3420 g         6264 t
ORIGIN      
        1 aggctctact atgattgaag aatatattga gaaaaatatt ttaagacagc tgtttttatg
       61 tgggcaattc tatgtaaata aagaagtcaa tctagaaaag ctctccaatc tcctgcatgt
      121 ttgcaaaacc actttactca atgatatcaa taacataaaa aaagaatttg aagaacagat
      181 cgcttataca catcgggaaa aggaccgtta cactttatat ttttcagaac atatacctcg
      241 atgcaagatc atgcagcaat tgtctcaaaa ttcgttgttt ttaaaaactt gtctgcttta
      301 tttggaagaa gatgaacctg attatcttca attaactgaa tgtgaattca tctctgtcag
      361 caaagcttat tcattaaaaa aacaagtatt agcttacttt aatgactgcg ggatcgaaat
      421 cgaccgctac tcccctcgat tcactgaaat ggaaagacgt ctgctgcttc ttaatgtgtc
      481 gtatcgttta ggcggtttta attcttggga gctacctgaa tcgttttttg aacgtgccga
      541 tcgtttcatt gaatccgtaa cggaaaatag tggcagattt tatgataaag aaaataaaga
      601 aatcttatcg attggttttg ctatcagttt cctacgccag caagtgtgtg ctgtgacaat
      661 agattcaaaa tttatcgaag aaatcaaaaa acgtcctatt tacaattatg tcgaatcagc
      721 atgggaaaac actgatttcc aaacctatta taaaaaagaa gaattcgcct ttattttgac
      781 actattcaat ctttgtaatt atggtttcca ttcttatcag ttgattgcgg aagactttca
      841 acagctacac caagtgttta ttgacaatac tcccgaaata aaagaactag ttgctacctt
      901 tgaatctcac ttcaatcaag aattattcgg aaaccaaccg ttcgaacgtg cgctgattca
      961 tctgatgcgt tcggcttggg ataactatca attattcatg ccagaaaaat tttatctttt
     1021 aaatgaggaa cagacaaatt tatataaaga agtccagaca attttttcca gctggtccag
     1081 ccagttacct tatgatctac ggttgaatcc taattgcatg cgggcttttg tgatcgaatt
     1141 gagcggaatt ctccgtctga caaaagaaca tctgactatc tatatcgtca caaattcgga
     1201 tgttcattat ctgatttatc gggaagcctt ggaagctgta actactttcg attttcaagt
     1261 tgcaccaaca atttattcaa gtatatctga tataaagaag tatgcgcagc agtcctcaaa
     1321 ccgggtatta tgtgaacgta cattatatac accagatgcg gtccaatatg aaaatattat
     1381 tcctatttcg atcaatacga tcgatcgagc tatcatttca gctgtgcaaa acaaataagg
     1441 ggctgtgaca aaaatcatgt cacagcctct tatttgttga agatgcccat ttctgtaaca
     1501 atctcttgat acacgtcagg ctcacaacct cacttatttt ggacatctag gggtccgatc
     1561 tctttattta aaaaataaga aatgatggtt cggataacta ccaacgccgc tagttggaaa
     1621 atatcctgca aggtaggttt gataatcgat tcgatgatcc cagctgaaac aagaatttcc
     1681 aaacttagaa gtatatagcc tcccaacatc tttttaatag cattgttctc gcttactcgg
     1741 tcgctttgca cttttgtatg gacaaataaa tatttcaaaa aatccttgat tgatagacaa
     1801 acaccccaaa ccaataaaca gatagcaaag atatcaagtc ccagtacaag tttttcgaaa
     1861 tatggaagaa gaaactgcat caactctgtc gtcatcctac ctctcctctt ctttgttttt
     1921 ctttgtcttc tatttcctga ttcaagaaat aagaaatgac tgttcgaatg ataataataa
     1981 gtgcaagctt caaaatatct gtccaagtcg gcttgataac tgactcgatg atatcagcaa
     2041 cgactagtac ttccaagctt aacaaaatat aacttgctag cagttttctt gtatctgcat
     2101 tacgagtatt acgttctgca tagtttttct tgacatgaga ccaatcaaaa agtttgaata
     2161 gaacaagaac gatcccgaca attaagatac cgatagaaca aatatttaaa atagcaatga
     2221 tccattctag aaattcaatt aaatttgagc gaaacatata cgctacctac tttcttttaa
     2281 tttgttccta ttcttttatt gtaaaagaaa gttaccttgt tcgtctattt aaaagttgtt
     2341 ctaatgataa cggttcgctt aaatttttct ttttcactct cccatttttc tccctccatc
     2401 cataaatcgt ggaagtatcc aaaacaacgt agcaagttaa aataaggctt agtccgtcat
     2461 cagcttttca aagaagcact gtctcggtgc tttttttgta cccaaatttc taaaaataga
     2521 accatacacc tcctcaaatc ttttcttctt acctacccta tttttattta caggaaaatc
     2581 agacacttct tcttttttgt caaaatttcc tttttcttct cgtcctatca tttggcattc
     2641 taataaaaac gaactatcct tggatataga aagagaaaac aaggaggcgt ttttcatgag
     2701 taaaaaatta atcaaagtcg gcatcggttt aggtttgctt gcccttggcg ctgcatatct
     2761 aggaaaaaag acaggactat tcgaagatga cagtcattta tatgatgaat tcgaatcaat
     2821 ctagcatcct atttagttga atcactacgg acatacttgg aagacattgg aggaatcaca
     2881 atgagaaaaa gtaaagcaat aatgattggt gccggtattg ccaatatggc agcggctgtt
     2941 tatttgattc aagaaggcaa atggaaaggg gatcagatca ccttttattc attggatgat
     3001 cacggttcaa atgatggtgc accgaccgac actgtagtgg atgaatattg gaataaaaac
     3061 catccattag aaaaccaaaa aggctatatt gctcgaggcg gtcgcatgct taattaccgt
     3121 acctatgtag atttgatgga tctgttaagt cgaatacctt ctgttactga gccagggatg
     3181 actgctgaag aagatacgag agattttgat gcaaaacacc gaacctttga caaagcacgt
     3241 ttgatggaag gtggcaaagg aatcattgat ggcggaaaac tgggattcaa taataaagac
     3301 cgtattcttc tgactaaact gattgctatg cctgattcag aagaagaaaa actagacaat
     3361 atcactatcg aagaatattt caaagatgat ccacactttt ttgaaacaaa cttctggttt
     3421 atgtgggaaa caacatttgc tttcagaaca cgtagttctg ctcaagaatt gcgtcgctat
     3481 atgcatcaaa tgatttatga attcactcaa atcgaacatc tagtgggcgt caatcgaaca
     3541 cgttacaatc aatacgaaag tattatgctt cctttgatcg agtacttgaa agaacaagga
     3601 tgcaaaatca ttttgaaccg ccgtgtcact gactgggaat tcaaagaaac accgatgcaa
     3661 gatgaaatca ctgtgactgg tttgaaaatt accaatacac tgactcaaga agaagaatac
     3721 gttgtggtcg atgaagatac agcagttatt ttcactaatg ggtccattac agattcagct
     3781 actttaggag attacgaaac accagctgta gaaaatatgg attatggtgc agcttctagt
     3841 ctctggaaaa aagccagtga acgtttctac aacttgggaa atcctgacaa attctttgct
     3901 gatcgagatg caagcgaatg ggtcagcttt accctgacga cgaaaaacca tttgttgttg
     3961 aatgaaatca cccgtatcac gactcaagaa ccaggaaatg ccttgaattc atttatttca
     4021 accacaccga tcacgccgtt aggccaaaaa gatgtcaata tgtctattgt cgtgcatcat
     4081 caaccgcatt ttacgtctca gaaaccaaat gaaacagtta tttggggtta tttcctttat
     4141 ccaagacgac gtggcgaatt tgtggataaa cagtatatta aaatgaatgg aaaagaaatg
     4201 ctagaagaac tgattggaca actttcaaaa gtagatcctg gaccagtgaa tattcgtgaa
     4261 aaagaaacag aaattttcga cagcgtaata aataatatcc ctgtctatat gccttatgct
     4321 tccgcgttgt tcaataatcg tgccaaaagc gatcgaccaa aagtcattcc taaacattcg
     4381 accaatctgg cctttacagg agaatttgtc gagcagcctt atcagatgat ctttactgaa
     4441 caaagtgctg tacgttctgg agaaatcgct gctttccatt ttgcgggtat tcctatgtcc
     4501 cgtcttgtca aaacaccgcg ttacgacaaa gatattccta ctctagctcg tgccgcaaaa
     4561 aaaatgtttg aataatcaag aggttgtgaa aaagctgccc tgcatcaagt aataagaaca
     4621 accaggctaa aacagcttct tatgtttttg atttggttgg acttattacc gcagatgcaa
     4681 gcttttgaac accgtttacc aagaggttat gaaaaagctg ccctgcatca agtaataaga
     4741 gcagccaagc taaaacagct tcttatgttt ttgacttggt tagacttatt accgcagatg
     4801 caagcttttg aacaccgttt accaagaggt tatgaaaaag gcgtttagtt tcgagtatga
     4861 agaaaaattt tgtgaaaatg gttcctcacg tttttgcata aatttgactt aataccgaga
     4921 aactgatccc ttctgtcata acctcttttt atttatgtct gtttcttttt gaaaacaact
     4981 attttttcaa cgtttactct cttgccaatc caacatcatc taacggatac aactatttca
     5041 atgattacca ttacactaaa aaatcggaaa cacacctctc cgaacacaaa tacataagac
     5101 tatagaatgt ttcatttaag aaaaaaagcg tttcattatc aaaaaggtaa gatctccttt
     5161 aaaatgaaat tatctgtagt actaataatg cagacaattt tatgaatctg aaaggagcat
     5221 ctccaatgaa agcagtcact tggcaaggaa aacagaaaat ggaaatcaga aacgtagatg
     5281 atccaaaaat attagaacca acagatgcaa tcatccgtat cacagcaaca gccatttgcg
     5341 gctccgatct gcatctttac catcatggtg aagcagtttt ggaaaaaggg tatgttgtag
     5401 gacacgaacc aatgggaatc gttgaagaag tgggatctca agttcaaaca ttgaaaaaag
     5461 ggcaacgtgt ggttatccct tttaatatta gttgtggtca ttgtcatttc tgcctgaatc
     5521 acatggaaag ccaatgtgat gaatcaaatc cagatcagtt atatggcggg cttttcggat
     5581 ttggcaagtt gaacggtaat cattggggtg gtcaagcgga atatctaaga gttccttatg
     5641 cagactctac ttcatttgtt gtaccagata atgatcttcc tgatgaaaaa gttttatttc
     5701 tatcagatat tttgcccaca gcttattgga gcgttgaaaa cgcaggagta aaaaaaggcg
     5761 atacagttgt gattctaggt tctggtccag tcggtctatt cgctcaaaaa ttcgctgtta
     5821 tggctggagc agagcgcgta atcgcagtag atccagtaca gcaccgtcta gataaagcgg
     5881 ctaattacaa tggggttgaa acttatttct tagaagacac ggcacaagct ggaacagaat
     5941 tgtatgaact ggctaaaggt ggcgcagatg tcattatcga ttgtgtagga atggacggat
     6001 tagagcctgt caaagaaaaa gcgaaaaatt tagtaagctt acaaagcggc acaatctctc
     6061 cattacagat ggcatcgcaa gctgtcaaaa aatttggaac agtccaagta actggtgttt
     6121 atatgactcc agcttcttct tacccgctgc aagaattttt tatgcgggat atcgctgtca
     6181 agcatggaca ggcaccaatt atccatctaa tgccaaaaat ttacgcaatg attgcagaag
     6241 gactattcga tcctacacaa atcattactc attcgctgcc actatcagaa gcagcacaag
     6301 catatgagat ctttgacaaa aaagaagata aaaatataaa agttgttttg aaaccttgaa
     6361 ttcagtaaaa aatacaggtg attttcttta tgcccaaaat caaacgaaat acccatgaga
     6421 tcgaaatagg aaaagaagaa gatatcgaaa tacgaggacg tcgcttccgc ttctatttcc
     6481 aaaatcgtta cacattgatc tcactggctg tcgatttatt aaccgggatt ttctatattt
     6541 ttggaagcct tgcttcctta actaaaatcc ccgatcgata tggcacctat ttttatttgg
     6601 ctggtgctat tttcttgacg attcgtccta ttctcagaat cgtaaggaac atttttattt
     6661 acgacgaaaa aaaagcaatg aagagaacaa gcaaaaaaaa agaggcatga gaatttttca
     6721 aatctcatgc ctcttttttc accctatcgt atcactttga tacagacgag cggtcgtccc
     6781 actttcttcc ttgaccattg tcaaaaatgt ccactcgtat cgttcataaa acgtggtatg
     6841 atctgtaatt aagtaaagtt tttcgtaccc aagttgatgc atttcttttc tgatttccgc
     6901 taatatccgc ttggctatcc cctgcttacg cttttccggt tcaacaaaca gggcacaaag
     6961 atttggtgtc agatcagggc ggttatggaa gtcgttttca atgacgcccg ctcctccgac
     7021 gatggtttct ttttcatcta agaccacata ccattgagga atactttcct tattagcgat
     7081 tccagtacga atactttcct catacacaac agcaggaact ccccattttg atgaaaacca
     7141 attcgcgctt tgttttacta attctgggtg ttctcttaat ttgatccagt tatatgtaac
     7201 caatgtttaa acctccgact aaaataaaac gtttacaaaa attatacaag tctttatgaa
     7261 aattagcaat gacaacaata gtgatgtaaa aaaaaaaacg gaaaacaatc acttgttctc
     7321 cgttttttga ttaatcaagc atctaaagct tcttttcttc tttcttggat cgtagctacc
     7381 ttgacgaacg gtagatagat caaaatagaa agaaccaaat tgacagcggc tagtacacca
     7441 cctgcaatac tttttgttgc caatacaccg ccaataattg gtggagttac ccatggaggc
     7501 ataacggtag ctgctggcac taaacctgta gaagtcgcaa agtaagcaac actaactaag
     7561 accattggtg tcaaaataaa tggaatgaac atgatcgggt tcaacacgat aggtaaccca
     7621 aacatcgttg gttcattaat attgaagaca cctggcgcaa tcgacaagtt tgttacaacc
     7681 atatatggtt tgtttttacg tcctactaaa taaatagcta acagtaaacc taaagttgca
     7741 cctgttcctc ctaagttgac aaagctgtca aagaaaggtt tgttcacgat aaacggtgcg
     7801 ctatgtccag ctgcgattgc attaacgttt gcttcaatgg ctggcgcatt gattgtttgc
     7861 atcaaaggat cgaccatatt cgctccgtgt aaaccaaaga accataagaa tggtgtgata
     7921 aatgcaagca gtaaggcaga tggataagag tttgcaagtc ccataaatgg ttcttggacc
     7981 actgtataga aagaacctac gatatcatca acaccgaatg cagcaaaaat tgcagcaact
     8041 aaagcaaacg cagagatagt gatcattgct ggtagtaatg aagcaaatgc ttttgcaact
     8101 gccggtggca caccatcagg cattttgatg atcaattttt catttccgct taaacgtaca
     8161 aagatttctg ttgaaataat cccaacaata atagcaataa acaaaccgtt tgagctcata
     8221 cctaatgctc cgcctaaagc gaagaaagag cctaaagaaa tcacacctgc ggaaacaccg
     8281 tcttttcggt agccgttagc taaatgataa gcaagcaaga aagcaatcaa gacagaaaga
     8341 atggcaaatg taccgttcca agcagcagcc ccgaatcctt tccatgattc accaccaaaa
     8401 attgagttca tcaattcttg gaacccggga atcggtaagt tattgatcat tactgctaat
     8461 gctcctaaga tcattaacgg catactcacc ataaatgagt cccgaatcgc aactaaatgc
     8521 cgttgtgcac caatttttga tgctacaggt ataaaatgtt tctccataaa tgcaacaaat
     8581 ttatccataa atcttccctc ttttctttac tgtagattac gcgttcaatc gacgatacag
     8641 atcaatgatc tcttttgcta aatctgtaaa agctatagat gtcatcagat gatcttgact
     8701 gtggaccgtc aaaagcgtga cttcaacgtg atttccttgt gcttcttgtg tcagcatctc
     8761 tgtttgagag tgatgtgctt ggactaaaga ttcctctgca tctttgattt tttcatctgc
     8821 taaagtgaaa tcaccttttt tcgcagcagc aatcgcttcc attgcatcgc tttttgcatt
     8881 tccgccaaac atgatcagac ccatgatggc ttctagattt ttttgatctt ccatttattg
     8941 tatcctccga taatttttat tatttgttta ttagtttttc tgcttggtcg agtacttttt
     9001 caccattcat catgccatag tcagccatat tgattacatc taaaggaatg cctttaggtg
     9061 ctagcttttc agcaaattga ttttgcataa agcgtacttg cggtcctaaa agtaaaacgt
     9121 ctacattttt agattctagt tgattatccg catctgaagc agatacagca aagatgtctg
     9181 cttccattcc ttttgcttct gctgcctttt gcatttttgt gactaggaga cttgtactca
     9241 ttccagctga acatacaagc atgattgttt tcttttccat tttaaacacg ttcctttcta
     9301 tacttacact tgtatataag caagtagcgt gccaacttta aaagcgcttt aaaaagttga
     9361 tatcatcaca tttaaggtct ataccaagtt gcatacacac taaaaaagaa agcgcacact
     9421 aaacacacaa attaattttt attacctttt ttcttttaac gtgcacaaaa aaagttgtga
     9481 cacaaaactt gtcacaactt ttttcaaata aacggtattc aaaagcttgc atctgcggta
     9541 ataagtccaa ccaagttaaa aacataagat gttgttttta ctttgctatt cttattactt
     9601 gatgcaggac agctttttca caacctctca ataaacggtg ttcaatcagc tgaccctggg
     9661 tattagctca aaacgctgga ttcacaacct cttttatttc aaataaccag taaatacaat
     9721 gaacatctac aaaaaacttc tatttattta tagatttcgt caggcaaatc caattctctc
     9781 ttatcaatca aagtctgata ccacgaagca gattttttca atgttctgct tcgttcatcg
     9841 ttcaattcga ttcgaactaa tccatatcga ttcttaaatg cattcattgg tgacacacag
     9901 tcagtgaatg cccataacat atagccttcg caatttgcgc cttcttctgt tgctttgatc
     9961 aagtaatgca aatgctgtcc gataaattcg atacggtaat catcttgcac gattcctttc
    10021 tcatccatat actgttcttc atattctctt cccataccgt tttctgttat cagccaaggg
    10081 atattatgat aattttcttt tagatacatg gctatatcat acatgatttt cggatagatt
    10141 tcccatcccc tggaattatt cattttcttc ccaggtaaat caaagacttc ataatatcgt
    10201 tcaggatgga aaggtgtctc ttcattccat tgataacgag gggcgcgaac gcgtttagga
    10261 taatattgat tgattccaag gaaatctact gtatgatgct gaatcgtttc taaatcctct
    10321 gttgcaggat cgaaataaat cgattgtttt tgacacaact ccaggaactc ttctgtgtag
    10381 tatcctttta ccatcggatc aaaaaatact cgattataaa acaaatcata catataagca
    10441 gctttttggt cttctggaga attggatcga gcatagacca tttcaggatt taaaatacag
    10501 ccaatccttc caccataatt tccgctgcgg taaatctcta ctgctttagc cgttgctaaa
    10561 actttatgat aattccacat catccacttt tttgtgtttt gttcatgcgg ccagcgaaca
    10621 gcatccaaat agcaacgcgt ttggggaacg attggttcgt tgaatgtaaa ccagtttttt
    10681 accctatcac cgtaacgatc aaaggcaatt tgagcatact tagcataaaa atcaactact
    10741 tttcttgaag accagccatc atatttttcc aaaagaaaag ctgggacttc ataatgttcc
    10801 aaacaaatca tcggttctac gtctgcttcg atcaaagagt cgatcatttc acttatatgc
    10861 tgagcatatt cttcatctac tatacagttc tcataatcag taaaaaaacg agcccaattg
    10921 atagaagtcc gataatgtgt cagatggatg gatttcatca gatccacatc ctgctggtat
    10981 tgttccataa aattagttgc tacggctggg ccatacccat tatgccaaac aaaatgttca
    11041 tttttatacc agctatctat aaacgaatcc tgtccttctt tttttccttt ccaaccttcc
    11101 gtttgccaag cagaagatgc agcaccaaga ataaaatttt ttggaatagt aattttcatg
    11161 atttcctcct gttttttaga tcccatcatt ttccacacga tttgctactt ttacaaatgg
    11221 cagatagatc aaaatagaaa caataataca aacaagttgc gtaataactg caccgatatt
    11281 tcctgctgtt gccagataag cattgacgat cggcggcatt ggccaaggga ccattacaac
    11341 tgctttcgcc gcaaatccta aagcggttgc aaaataacca atcgttccag tgataagagg
    11401 cgttaagata aacggaatag ctaaaattgg attcagcatg atcggcatac caaaaataac
    11461 tggttcattg atgttgaaga tcccaggagc aagggataac ttggtgattt gtttcatatc
    11521 ttcccgtttt ccaactaaca atactgcaat cagtaaaccg atcgtgactc cagacccgcc
    11581 catactcata tacatatccc aaaatggcat agtaataatg ttcggtactt cttttccagc
    11641 ttcaaaagct tccgtattta cagcaattgc cgctaaaagc aacggttccc gtatcggctt
    11701 caccatttga tttccatgga taccaatcac ccaaaagact tgcgcaataa acatcaatag
    11761 caaaataccc ggtaaccctt gtacgacacc ttctaatggg cgttgtacga cattgtaaat
    11821 gatatcataa aggtacattc ctgtaagttc tttgatgata aatcccgcag ccgcgatacc
    11881 cgatacagtc agaatcgtcg ggaataaagc agaaaaacta gcagaaacat tcgttggcac
    11941 ggtatcaggc attctcagct gtagtttctt ctgtttcctc aaccagcaat aaagttcaat
    12001 agaaaggatg gagaccaaca ttcccaaaaa taaccctttc gtatctgtat attgtttagc
    12061 caatacgttt tctactataa cagtctgatt ttcattcact aattcataaa cagttggatt
    12121 aacagataaa taagacatca ctgcgatgat accagggaat acaccatcca ctttattcag
    12181 cttggctaat tcgatcccta tcagaaaaac agcatagatg gtcaagaaac ttagtgtgac
    12241 atagctgatg gcagatgcga taggctttaa ttcttctaaa aaacgcaaga aacttatctg
    12301 tgctaagcca ttttccgagt caaagaccat tgctgaaaac aatgtgccaa aagcacccgt
    12361 aataattacc ggaatcaatg cagcaaatgc gttttttatc accatgatat accttaaatt
    12421 attgattctc gttgccacgt tccctaaaac attggctaat ttatccataa aactcattcc
    12481 agatcccctc attcctgctt cttctccttg acgtcatctc tttttgtcta ttagtcagcg
    12541 cttacatttc gatgaacaga aactatttac tcctcttttt taccaaatat aaaacaaaaa
    12601 ttcatctgat gtatttatca taaaatacat cagatgaact gtcaattagt ttctttttct
    12661 cctattattt tctgtaattc tctccgattt attgccatat ggacagtcat tgcatcaaac
    12721 gcagcaagtg cattccgcct ttcgatggcc tgaacgatct ctgtatgaat atcgatcgtc
    12781 tcgattttca gatgctggat atcataattt ttattaaaca aagcaattga ttcattaatg
    12841 attgggataa tatgcagcaa ggctacgttt ccgctagctt ctgcaatcat cgtatgaaaa
    12901 tcgatgtcta attgaacatg ttcttgtgtg ccttctcgaa aagaagtttc aatcttttgc
    12961 gttaattttt tcatttggac gatttgattc ttcgttgcat attccgctgc caaacctgcc
    13021 atcttaggct ccaataaata tctcagttca aataagtctc gtatcatccg atcctgatct
    13081 tcaatcaaag aaaaaccgag tgggtcattg gcaatccctc gcttatcact gacaaatgtc
    13141 cccgctcctt gccgaacttc aaggatattc ctggaagcta gagcccgcac agcttctcgc
    13201 accgtgcttc gccccacctc taaataatcg gacaattcat attcattcgg cagtttatct
    13261 cctacttgca actgcatttc ctttaaatat ctaaggatct tgtcagtagt tacttctact
    13321 agggttttat tttcttccaa accacatacc tccttttcat ctgatgtatt ttttcgtaat
    13381 tattataaca cgtttgaatc atctgatgat tttttgtata caaaattgat taatccgttc
    13441 atagacatac aaacgaagaa gatatttacc ataaaaaaga ctaggataaa attcactcct
    13501 agccttcgat catttttatt ttgaacgaca aaaaatatga tgttcaataa cagacgatct
    13561 tcattcgatt cttgatgtca tcttttgttt tttttaatat atggataaaa agtttgatta
    13621 catcgtttct attctagaat ttggtcgatt ctcaattctt cttctggtga gaaatctaaa
    13681 ttatccatca ttcgaacact ttcttgtaac tgacttaacc gacttgctcc gaccagtaca
    13741 cttgtaacag atttttgtcg caaattccat gcaagggcca tttgcgctaa tgattgtcct
    13801 cgtgatacgg cgattgtttg cagtgctttg attttttcca gagttgatcc aacttgttct
    13861 tctgaaagga atggtatttc cttacgatgt gctctagaat cttcaggaat accatgtaga
    13921 taacgatttg tcagcaaccc ttgagcaagc ggactgaacg tgattgcccc tagcccttct
    13981 tcttctaata ctggagatag atcgtcttct agtagtgcac gagaaaacat attgtagcgc
    14041 atctgatgaa taatgaacgg tgcttccttt ctttttaaaa tttcagtcat tttttttgta
    14101 tcttctccat tatagttgga tataccaata tataatgctt ttccttgtct tacaagttgc
    14161 atcaaagctt ctgctgtttc ttccaaaggt gtatcagggt ctgggcgatg atgatagaag
    14221 atgtcaacat aatctagccc cattctttgg agactttggt cacaacttga aatgatggat
    14281 ttacgagagc cccattcccc atatggccct tcccacatat gataacctgc tttgctagaa
    14341 ataatcagtt catcacggta ggcttgaaaa tcttctctaa aaatacgccc aaagtttttc
    14401 tctgcagctc cagcagggcc tccataatta ttcgcaaggt cgaaatgggt aattcccatg
    14461 tcgaatgcgc ctcttaaaat ctctctttga ttatggatag gatcataatc accaaaattt
    14521 tgccataatc cgagagataa aatcggtaat ttcaaacctg aatgaccgga tttacgatat
    14581 tccatttttt cataacgtgc actatctgca atatacaatt tttctcctcc tcatcaaatc
    14641 tttttaaaac gctttctttt ttctattttt agtttactac attttaaaga aaaggagtta
    14701 atcaaaatga ttttttcgta tatttttttg ccatttgttt tttactcgtt gatagatcag
    14761 aaattgatta cgtgcttgct ggattttttt ataacgtttc ggttgttttt tgtaaaattg
    14821 ctgatgatag ttttctgctg gccaaaacgt accggctggt tcgatttttg tgacgattgg
    14881 ttggtggaaa gttcctgaac tgtctagttt ctgttttgat tgttccgcta attctttttg
    14941 tcgttcgact tgataaaaaa tcactggacg atattgcttc cctctatctt gaaattgtcc
    15001 aaatgtatct gtgggatcag taatttgcca atagattgtc agcaactcgc tataacttat
    15061 ttctcttgtg tcataaatga tctccacagc ttccacatga cctgtgtaac caccacttac
    15121 ttgatcataa tttggttttt caacatgtcc gcccgtgtat cctgacaaaa cagataggat
    15181 cccttttttc gattcaaacg gctcgaccat acaccagaaa catccccctg caaatactgc
    15241 acgttcttga tagtcagcat cttgcatggc atgcttggaa aagtcatatt tttgctcctt
    15301 atcaaatcct tccgtaatca ataaataaaa gtccattaca tcaggcgtca gattcattcg
    15361 gatagctaat ggacgtagtg ttgcttctag ttttgataac tgttctttaa cggatatatt
    15421 ttctcttatg tcattctttg tctgaatcaa tacttttcgt tcccaatctc gtgtattcgg
    15481 attcaaaatc agattataaa gtgtttgaat gaatatttgt tcctcatcca tagattctcc
    15541 tcctttccga agaagacaag ctagtgactt tagctgtaat ttatcataaa gtgaggaaat
    15601 aagcgattac ttcatccccc tacagatttt tcgtataaaa aagagagaat ccttatcgac
    15661 agattctcct ttttaatcaa tttattttat aaatgaaaag ttaatttacc ttttatggaa
    15721 aactatttta aaaaataaac ataaatatgc agttaatgta acaagataag aaataatagt
    15781 cgttactgta taacttgtgc ttactttaat atcagcaact cctttgactc cgcttatttg
    15841 taccaatcca ttagcattca cctgctcctt tacaggttga tgatttattt ctacattcgt
    15901 tcctaaataa gcatatacag gaagtgtgat tacttggctc ttctcattag ttactctata
    15961 tatgatagaa ctaccagttg ttttataagg aaggtttacc cattgactgc caatctttgc
    16021 ttcgtgtttt attatatgct ccttattctc caatgaagcg ataggagaat aatcatataa
    16081 aaaataatcc attcctttag tggtatacgc ctctactgtt tctttgttta caacaatatg
    16141 tcccggctga gaaattattt tttgattgaa atttttttta ctagaaacag ttaaagtgat
    16201 taataacaaa acagtcataa ataaataaaa tagttctctt gtcttattgt atcttctatt
    16261 tttgaaaaat atcatcgtta atatcagaga agagaatatt atttgcaagc ttaagatccg
    16321 ccaaggaaat tgtaacaatt tggctggcgt attttgcaga agataccatg gcaccaaact
    16381 agttgtagct agccaagtca aacacgtacc ataataaatc tttttttcta tgtttgttaa
    16441 ttttttaaat aaaatcaggg gaatacataa agataacaga ccaactagtc ccattgagta
    16501 agtagttaag tcgttgttca tagcttcagt cagactatcg aacacattag atgcatgagc
    16561 atataaattt gtcacgctag gtttgttgat tttttggtag agcatttgtt caaacatcgg
    16621 ataccaaaaa gcactcgtca gaaccaatgt cattcctgcc gcactaaata attttttaaa
    16681 tatagcaata aaatctgcat tcttgaaacc tttagaaacc aaacgaataa aaataaaaat
    16741 gataattaat gctgtactca ttaatgttga tagtacatgg ctgtacacta gcaaactcat
    16801 actcaatgct agctttacag acggatactt ttcttctttt ccagagatga tttgataagc
    16861 ataaaataat acagggataa caagtgttat tgcgatagct tcggcaattg cggaacgata
    16921 gtagatgtct actgtccgat aagcagagaa aatatagaga caactaaata aaaataagta
    16981 gttcagtttt ggagtacaaa aagtgaaaat gtaaacctaa atgagtatca aattgcaact
    17041 gaatttgata ctcgtttttt attgttaaat caacgtttgt tgttaattta caccacaaaa
    17101 tgtaagttaa aatgtatata aaaatgcgaa cgtgaaataa ttattgtagg aatgcaaatt
    17161 ggtgtgttaa aatggttaca atttactaag caaaaagggg tgcattttcc tatgcaattt
    17221 cttgagccaa aaaataagaa cgctaagtct gtggattggg aaatatcaga gcaagtaagg
    17281 gtgatagtta agcaatacgc tgaatatgca gagcgtactg aaagcgaagc agtggatgaa
    17341 ttcttattga acattttaga cgataagaag tttattgaat ggattgccaa caagcgaagt
    17401 aataagcgga ttgttgaaaa aatgggtatc aaagatagag tgggttgata gacaatgacc
    17461 aagcttaaaa gattagccac gaaggaagat gaaattgtag atgttaaaat tccgatttca
    17521 aacgaagaat taaaagatag ggctaaacaa tatgatttat taactccaaa acgatttgca
    17581 acaaggtata acaaaatgtt gttcttgcca acgtctttta aatggaacgg ttctgaatat
    17641 cccattcaat ataattactg catcaatccg ttttgttgta attttgggaa agaacagcac
    17701 aaattcaaag atgtaaaagg gaaaccaagt cgatataaaa tgactggtag ttccaaagat
    17761 aaaggtcata aaggtatgta ctgcaatgac aatcctattg gcagaggtgt atcgcaaaac
    17821 tgtacagtaa cccctttatc taattggtct gttgttgaag aaattaaacg ccttatagaa
    17881 atcaatagca tccaagatgt tgagccagat tatcaatttc ataaagaagg ctgctcggaa
    17941 gaagaatcaa ccccattcaa tgagccgaag caattttaca aaagggggaa aagtaggggc
    18001 aaatctcaac gctatcaatg taaggcgtgt aagaagttca ctaatgttct accaaaaaga
    18061 gaagaaacca ctacttatca ccaacaaaag aacaccattc ttccaatgtt cgctaagatg
    18121 gttgttggaa gggtttcagt cagtcgtact tgtgatattt taggaattgg agttggaacc
    18181 tactatcata agctggagtg gctgtatagg cgttgtttgg agtttttaga acgatatgag
    18241 acacaacccc ttcaaacaaa gaaatttaac gagatgtggc ttaatacgga caagatgcac
    18301 tactacctaa ataacgtccg taagaaagga caaggttcaa agaaatatac ggggtttgaa
    18361 gaccttaata tgcaaacgta tatagttgta tctgctgagg tcttatcaag atatgtgttc
    18421 cgttctgatg ttgcctatga ttggaatatt tcaatggatg aattaaatga agatacaaga
    18481 aaattcaaag aagaccatct caatactttt agtcgtaaaa atgataggtt agattggtct
    18541 tattatccac aagaaccatc cgctaacgat agcgaaaata ggaatgctta tcttcacgaa
    18601 ttagggaaaa ttactaatag gagtagattt gtcgatggtt taaatgttga tgctccttat
    18661 actacaactg ctcactattg gttaattaaa caaatggtta atgccgacga atggcgaatg
    18721 attagtgatg atgacttctc tattagaaac gccttttata gagtattcac gaaagaatta
    18781 aggttgagcg atgcacatca ttttatttgc caagttaaca agacgaaaag cagaaaacaa
    18841 tgcttaaaag aattcggaca agcaaaagca gaactgctcg attggggaga tatcagagga
    18901 ttcaaaacaa agtttctacg gactttggca tctcattatc taactgaatt actaacaagt
    18961 catcaattcc acgaagaggc tattagtaaa gatggagaaa gatatcgcaa atatgccgat
    19021 aaccctatca aacatccatt agccactaag gataaaggat tttattcagt tgattgtaga
    19081 acggatttat ctgctctgga accaaacgaa atcgcaaaga tgctattaaa tgttaatgac
    19141 cattcgacca atagctttat tcaacagata agacgttata tatcatctct tgaaagacca
    19201 ttaacaacag ctcgtggtga taagaagagc tatatttacg ctaacttcaa tccgaagtat
    19261 gctcaatttg cgattacaat tctaagaact tactacaact tttgtcgccc ttttaaatca
    19321 gcggataaaa aggttttgac acctgctcaa agattaggga ttacagataa acaatttgat
    19381 tggaaagata taatttattt caaatgacaa aaagagcttg gagtaatccc aagctcttgt
    19441 gctttatctt tactgttctt cttcaattaa cgcacccgtt aactcaataa gttttttctt
    19501 tcatatacat gaaaatagta gttatacgga tttttatcat tctttatccc tttttgggca
    19561 aatacctcat tccattcctc ataattcact tctggaaaaa aagtatctcc ttcgaattca
    19621 tgatgtattt ttgtgatgta cattttctca acataaggga aaaacaaatt ataaatctgt
    19681 tctcctccga aaataaaaat ttcttcttcg tttttacata actcaaaaac atcttctatt
    19741 gaatgaacaa tttcacaacc attaaaggta aaccccttat ctctcgtcag aataatattt
    19801 cttctgtcag gtaaggctct tccgattgat tcaaggttct tcctacctaa tattatcgga
    19861 tgtccctttg tagtattttt aacatattcc cagtccttgg gaatcctcca aggaatgtca
    19921 t
//
LOCUS       DACDPS010000045        19648 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.045, whole genome shotgun sequence.
ACCESSION   DACDPS010000045 DACDPS010000000
VERSION     DACDPS010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19648
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.045"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            219..419
                     /locus_tag="IWJ07_001722"
     CDS             219..419
                     /locus_tag="IWJ07_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9333302.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            566..3355
                     /locus_tag="IWJ07_001723"
     CDS             566..3355
                     /locus_tag="IWJ07_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP9333303.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            3402..3929
                     /locus_tag="IWJ07_001724"
     CDS             3402..3929
                     /locus_tag="IWJ07_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP9333304.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            3950..5140
                     /locus_tag="IWJ07_001725"
     CDS             3950..5140
                     /locus_tag="IWJ07_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9333305.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYSLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5180..6478
                     /gene="asnS"
                     /locus_tag="IWJ07_001726"
     CDS             5180..6478
                     /gene="asnS"
                     /locus_tag="IWJ07_001726"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAP9333306.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            7531..8205
                     /locus_tag="IWJ07_001727"
     CDS             7531..8205
                     /locus_tag="IWJ07_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP9333307.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            8202..8543
                     /locus_tag="IWJ07_001728"
     CDS             8202..8543
                     /locus_tag="IWJ07_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333308.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            8573..8932
                     /locus_tag="IWJ07_001729"
     CDS             8573..8932
                     /locus_tag="IWJ07_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAP9333309.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            9135..9314
                     /locus_tag="IWJ07_001730"
                     /pseudo
     CDS             9135..9314
                     /locus_tag="IWJ07_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            9456..11537
                     /gene="cadA"
                     /locus_tag="IWJ07_001731"
     CDS             9456..11537
                     /gene="cadA"
                     /locus_tag="IWJ07_001731"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP9333310.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            11848..13314
                     /locus_tag="IWJ07_001732"
     CDS             11848..13314
                     /locus_tag="IWJ07_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9333311.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            13929..14468
                     /locus_tag="IWJ07_001733"
     CDS             13929..14468
                     /locus_tag="IWJ07_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HAP9333312.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            14542..14982
                     /locus_tag="IWJ07_001734"
     CDS             14542..14982
                     /locus_tag="IWJ07_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAP9333313.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            14995..15204
                     /gene="copZ"
                     /locus_tag="IWJ07_001735"
     CDS             14995..15204
                     /gene="copZ"
                     /locus_tag="IWJ07_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAP9333314.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            15204..17390
                     /locus_tag="IWJ07_001736"
     CDS             15204..17390
                     /locus_tag="IWJ07_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAP9333315.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            17410..19581
                     /gene="copB"
                     /locus_tag="IWJ07_001737"
     CDS             17410..19581
                     /gene="copB"
                     /locus_tag="IWJ07_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAP9333316.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
BASE COUNT         6566 a         3414 c         4293 g         5375 t
ORIGIN      
        1 aaaaaaagtt cggtttttca ctttatgtaa tcgctgagaa aacttttttt aataaaaaaa
       61 gtcgaaaaac tctagctttt ttgtaaacgg tatgatatac tataggagta gtttttgttc
      121 ggtaatctgt ggtagcttgt ttcaaataaa catcttccag atatacacca ttcaagtaat
      181 tgcaaaatat gtgttgatac acaaggagga tttttattat ggaaacaggt acagtaaaat
      241 ggtttaactc agaaaaagga tttggattca tcactgcaga aaacggaaac gatgtattcg
      301 ttcatttctc agctatccaa ggcgacggat tcaaaacttt agaagaaggc caagcagtaa
      361 ctttcgatgt tgaagaaggc caacgcggac ctcaagcaac aaacgttact aaagcttaat
      421 taacctatac taaaaaataa acgcccagac atttgcgtgt ctgggcgttt ttgtctattt
      481 tctttaaaaa gcgatgagag tatttttctg cactttgtag tataatttca aaagaacatt
      541 agaggcagga gaagaggaag atattttgaa accaagaact tatgcagtcg ttgatatcga
      601 aacgactggg acgaatccaa aagaagatcg gatcatccaa tttggctgcg tcatgattga
      661 atcaggtcgg atcacttcac gtttttcgat cgatattcat ccagggcgga agatttcaaa
      721 acaaatccag catttaacag ggatcacgaa tcaacgcgtc caaaaagcgc cttatttcga
      781 agatgtcgcc caaacgattt acaatctttt ggcagacacc atatttgtcg cgcataatat
      841 ttattttgat tataattttt tgaaccatga actcatgcga tgcggacttc ctagtttgaa
      901 gataccaggc atcgatacag tagaacttgc acaaattttc ttgccgacgg aaccaagctt
      961 tcgcttagct gatttgtcag aaagccttgg atttattcat gagaatcctc atcaggcaga
     1021 tagtgatgcc gaagtgacgg gtcaacttct tttgctgatc gaagaacgga tgagaaaatt
     1081 gccaatcatc acgttagagc agattgcgcg attaagtaga cacacaggta tggatacgag
     1141 tcgtttcatt tatcatgtga ttgaagaaat gaaagaaaaa ccagagccac ttgatccctc
     1201 attagaaatc gtcgatgggc tagctttgca gaaaaaagaa gtggaactat tcacatccgt
     1261 ccattatggc gagcggacat atccaagaaa aaaacaagca aaagaaaaaa tgtttggtaa
     1321 aacactgatg tatcgaaaag aacagaacag actgatgaat gcggtctacg accatttcac
     1381 aaaagatgaa tcaaaagatt tgatgatcga agcagctaca ggaatgggta aaacaatcgg
     1441 ttatctgctg ccattgagtt atttggctac acctgaaaaa cccgctgtca tcagtaccgt
     1501 ttcgctagtt ttgcagcagc agatcataga aaaagacatt ccattgctga atcaattgct
     1561 agatcagccc atacaacctg taattatcaa aagctatcgc cactatattg atttacagcg
     1621 ttttaaaggc acacttgtgg aaccgccaga acaaaaacaa tatgctttgt atcaaatggc
     1681 ggtcttagtt tggctgacgc aaacgaagac cggtgatttg gatgaattac atttaacaaa
     1741 tttgaaccat tcattctttg cagatatcgc gcacagaggt attggatttt tagcaagaaa
     1801 tcagtcattt tacgaacaag actttgttcg tttcttgcaa aagaaaatca ggcaaagtaa
     1861 ttttttgatt gtcaatcatg cgtttttaat acaagaaaca cagcgaaaag aaccattgct
     1921 tccagctagt ccatttttgt tgatcgatga agtacatcat ttgccggaaa tcgctgaaaa
     1981 agtaaatgac aaacgattaa gtattaacta tttccataaa caaatccagc aattagaaga
     2041 accaaatcag ctgtttgacc gaatacaaag ctatttaccg aaagaacatg aagccttacg
     2101 ctggttatcg atttatcgag aagaactttc tgcgctatgc gaagcacaga tttgtatggc
     2161 agaaggatta atgagtttag tgagcggcaa gcaacctaac ggacagtatc cagaagaagt
     2221 catgatgaca aaagagatgt tggatcagct gccactggaa tctgggacag ccattgatca
     2281 cctgcagctt ttttatcagg agatccagca gctacagtca caaatgcgtc tgacttttga
     2341 gattatccaa gaaagctgga caaaccagat gcggatcgat ttagcaagtt tatatagctt
     2401 atttgatcaa ttagacaagc agtacttgct gatggatcaa tggctggaag actggcgaga
     2461 tcatttgatc cattggttcg ttcctgggaa cacagcgcag cagatgattt ttcatctcca
     2521 tgattttcaa gcggctatgt tgccgtcgac tgtttggtat cctcgatatg atcgtattct
     2581 ttatattgga ggaacgctga agatcggccc taatcgacac tatctctcta agcggcttgg
     2641 cattgaagca gcacctctta aagtgattgc ctctccttat gattatgaga aacaggcaag
     2701 gatctatgtg ccaaaagagg cacctgccgt ccatcaagtc agcaatcaag catatatcga
     2761 ttatttagag acagcactta caggtctgat cgaacaagaa gatcggccaa tacttgtgtt
     2821 attcacttct catgatgttt tgcagaaggt ctatcagaaa atgcatctgt ctatgctaga
     2881 aaaaggacga gaaatactag ctcaagggtt tggcggaagc cgggagaagc ttttgaaacg
     2941 cttcattttg tcagaaaaaa gcgtattgtt tggcgctgac agcttttggg aaggtgtaga
     3001 tttgcctggg gatgctttgc agatcgttgt agtcacacgt ttacccttcg ataatccgct
     3061 tcgcccgatg gtcaaagcta ggaacgctta tttagaatca gaggggatca atccttttta
     3121 ccaggaatcc gtacctaaag cagctttgaa gttacgccaa gcattaggtc gcttgataag
     3181 gacggagaca gatcgtggcg tattgcttat tttggatcga agactagtca ctgcaaaata
     3241 tggtgcacgg atcatccatg cattaccgaa aaaagtaccg atcaaagagc tagctatgga
     3301 ggaaatctat caggacatcc atgaattctt aaagaaaaat gaagaaggaa gttagaggag
     3361 ctgtcttttt tcataattaa aatctgctat aatgaaaagg aatgaagata aaggagcgac
     3421 gacccttgaa gaaaaaacag acaaaggaaa aaacgattaa tcgcgtgtta cttggcttga
     3481 tcgtattttt attagcggtc atcgtgatca gctcgatttt ttacttgcgt tccagtcgtc
     3541 cggcggtgca ggcgaagaaa gaagcagtag aaatcgcaaa gaaatatgca aatcttgagg
     3601 aagccgatca tttttattgg tttactcgaa agaagacata cttttcactg acaggtacaa
     3661 atgataaagg aaaagaaatc gccgttatca ttcctaaatc tggtgaaaaa gtaaaagtat
     3721 tggatcaaac aaagggactg actgaaaacg aagcaaaaca gacaattgct gcggcccatc
     3781 ctgaaatcca aatcgaaaaa gctgctttag ggatgtatga tgatcagcct gtgtgggaag
     3841 tagtaggaaa agaaacaaat gggaaactga attattacct gattggcttt gaatctggag
     3901 atgaagtcaa gacaatcaag ggaatttaaa aggtaaagga atgaatggga tggagttatc
     3961 aaaaagaaca aaacgattag aaccgtctgt cactttggca gctgcggcaa aggccaaggc
     4021 attaaaagca aaaggcagag atgtgctgag tttgactgtg ggagaaccgg attttgcgac
     4081 accagaaaac atccaagaag cagcaatcga agcaatccgc aacgggaaag ctagctatta
     4141 tacaccaaca gcaggaatcc cagagcttcg acaggcaatc gttgattatc tgaagaaaaa
     4201 ctatggactt gtctatgaac cttcacaaac agtcgtaaca gacggagcga aatttgcttt
     4261 gtataccctt ttccaaacga tccttgaccc gcaagatgaa gtcatcattc ctgtgcctta
     4321 ctgggtcagt tacggtgagc aagtaaaact ggcagaagga cttcctgtct ttgtgaaagg
     4381 ggaagaatcc aatagctgga aagttactgt tgaacagcta gaacaagcac gttcagaaaa
     4441 aacaaaagcc gtaatcatca attctccatc gaatccgact ggaatgattt atagcaagaa
     4501 tgaactgcag gcaatcggtg aatgggcagt aaaaaatgat attttgatag ttgctgatga
     4561 tatttatggt cgtttagttt ataatggcaa tgagtttact ccaattgcaa cgatttcaga
     4621 agcaatcaaa aatcagacta tcatcatcaa cggcgtgtct aaaacatatg cgatgacagg
     4681 ctggcgcatt ggttatgcgg tgggaaatcc tgtgatcatc aatggaatga tcgctgttgc
     4741 ttcccagtca accagtaatc cgacagcagt cagtcaatat gcagctgttg aagcattgaa
     4801 aggcgagcaa gatacagtgg aagaaatgcg gaacgccttt gaagaacgcc tgaatacgtt
     4861 atattcgctt gtggcagaac tgccgggtgt ttctcttgaa aagccacaag gagcttttta
     4921 tctttttcct aatgtaaaag aaacgctacg catgtgtaaa tatgaaaatg tcacacaatg
     4981 ggtagaagat cttttagaag aaacaggggt agcgcttgtg actggcgaag gctttggggc
     5041 accagaaaat gtccgcatga gttatgctac tgatcgcgct acgttagaag aagcggtacg
     5101 cagaatcaag caatttattg agagtaaaag tcaaaattaa tcctaaagaa attcaaggaa
     5161 tataaaagga gagtcatacg tggaacaaat tcaaatcatc gattcgaaaa accatgtggg
     5221 agaaacagta acaatcggcg cttgggtcgc aaataagcga tcaagcggaa aaatcgcatt
     5281 tttacaatta agagacggaa cagccttttt ccaaggtgta gttgtaaaaa gcgaagtgtc
     5341 ggaagaagtt ttccaaacag ctaaaaatct acaccaagaa acatccgtgt tagtaactgg
     5401 agaaatccgt gaagacagtc gttcaaaatt tggttatgaa atcggaatta caaatgtgga
     5461 agtggtaagc gagagccacg attatccgat cacaccaaaa gaacacggaa cagacttctt
     5521 gatggatcat cgtcatttat ggctgcgttc ttcaagacaa catgcgatta tgcagatcag
     5581 aaatgaaatc attcgtgcaa cgtatgaatt ctttaatcaa catcattttg tcaaaattga
     5641 tccgccgatc ttgactggtt ctgcaccaga aggcactacg gatctattcg aaacaaatta
     5701 ctttgatcaa aaagcttatt tatctcaaag tggccaatta tacatggaag ctgctgctat
     5761 ggctttcgga aaagtctttt cattcggtcc gactttccgt gctgaaaaat caaaaacacg
     5821 acgtcatttg atcgaattct ggatgatcga accagaaatg gctttcactc atcaagaaga
     5881 aagtttagaa gtacaggaac aatatgtagc attcttagta caaaatgttt tagatcattg
     5941 cgaatatgca ttagatgtac taggacgaga caaagaagtc ttaaaaactt atacaaaatt
     6001 gccattccct cgtattactt atgatgatgc gatcgaatta ctgaagaaaa atggatttga
     6061 tgacatcgaa tggggcgaag actttggttc acctcatgaa acgttcattg cgaattcatt
     6121 cgaacagcct gtctttatcc taaactatcc aaaagccatc aagccattct acatgaaacc
     6181 acatccaaca agagaagacg tggtgatctg tgcagatatg atcgcaccag aaggttacgg
     6241 tgaaattatt ggcggaagcg aacgggcaac agactatgat ttcttgctag aacaaatccg
     6301 taatcacggt ttggatgaaa aagaatattc atggtatctt gatctacgta aatacggttc
     6361 tgttccgcac tctggttttg gtctaggtct tgaacgaaca gtcacttgga ttgctggtat
     6421 cgaccatgta cgtgaagcta ttccattccc acgtttgttg aaccgaattt atccataaga
     6481 ggtgatgaaa aagctgttct gcatcaagta attagaacag ccaagcaaaa acagctgttc
     6541 atgtttttga attggcggga cttattaccg cagatgtaag cttttgaata ccgtttagtg
     6601 gagaggttgt gaaaaagctg tttagtttcg agtattaaga aaaattttgt ggaaatggct
     6661 ttccacattt ttgcataaat ttggcttaat accgagaaac tggcttttga acaccgttta
     6721 aggaagaggt tgtgcgcctg ccgttcagct ccgagtcaca aggagtaatt tcaaaaaaca
     6781 gtttttcatg tttgatgact attgtgactt gtgaccgagg agttggccgg agagcaccgt
     6841 ttattctaaa aaagaactgc gaactctcgc agttcttttt ttaaaatacg ggataaagga
     6901 agtttccagc agacaagaga agaaagcaaa agaaattgct atttgattca caaggttcta
     6961 taacgaaatc gcaatcaatc aaacatttac aaagaaggta attcaaatgg aaatctgatt
     7021 tgatgatgct tctgctgttg aaataaagca gcatctagca cctgaaaaca aactaatagt
     7081 aacattcgaa gatggagtag gtccgtattt tcagcatgca ataatccata tgtaagttca
     7141 attcaccctt gaaatcatcg gaccagataa agttatgacc aatctatccc aagcaatcta
     7201 gaagagatat tagtaaaggg acatttacta gaagtcttag atgagcctat aaatatatgt
     7261 tctgatggcg tcatcagtgg tctgattgac ggaagcttag gtgtgatcga tctctctgga
     7321 ccaaacaacg aaggaatcaa ggggaatccg gcacgttaag aaaaaaatag cataaaaaac
     7381 tagacacttg atgagcgttc attcaattgt ttgaagggac taggctggtg tcatttttta
     7441 tacatatatt ttctctttcg tcttgatagg gcttatggga taatataagg aagaaaatca
     7501 tcgttttatt caaaaaggag tttttgacaa atgaaaaaac gtatattatt tatcggtgat
     7561 agcattacag acgcaggaag agaccgagca gaagcaactg atctaggtaa aggttatcca
     7621 cttttgattg ccggaaaact aatctcccaa tatccaacga tggaatttga atgcatgaac
     7681 cgtgggatag gaggagacaa aattgcagat ctgcaagtac gctggcaaga agattgttta
     7741 gctttacaac ctgactatgt atcgattttg atcggaatca atgatacctg gcataatact
     7801 agcgatcagg ctatattcgg aactgagaaa tctgctcagc aatttgaatc cgtttatcga
     7861 aaattactaa atgaacttca aacagtggga atctgcaaca tcatcttgat ggagccgttt
     7921 gtacttccta taaccgacga ccgtcatggc tggcgtaaag acttagaccc taaaatccaa
     7981 attgttcgta aacttgctaa ggaatatggc gcattgctgg ttcctttaga tggtatattc
     8041 aatgctttag ggattgttta tggtttttcg aaatatacaa gagaagatgg ggtccaccca
     8101 acgattggcg gacatgagat cattgcacaa agctggatag atgcaattgc cgcagtcgat
     8161 ttttttagtg gagataaaaa tgagcgaaag aaaagtgaga aatgatggaa atacggtggg
     8221 cagtaaaaga agatctacct caaatcaatc agctgattac tgatcatcca gaatcattcg
     8281 taaaagcaaa aacggcagct gacctaaatg ataagaataa actattgctc gttgctgtaa
     8341 caaaagaaaa acggattatc ggatttgcgc aagcggacgt gcaacagtca gaagaacgat
     8401 tggatcagcg accatcagga aaactaatcc aacacacatt agtaacaaaa gaaaatacgg
     8461 aagaaatcca aaaaaaattt gcagaatatt tgatactgga agctaaaaaa agaagaataa
     8521 aatcattttt tgcccagttt taatcaaaaa cagtaataga aggagaaata caatgagttg
     8581 tttaggaaat gtcatctggt ttatattcgg cggactaatc ggtggtctta gctggttagc
     8641 agcggggata ctttggtgta tcacgatcat aggtatccca atcggtatgc aatgttttaa
     8701 gatcgcctcc ctttcttttg cgccttttgg caaagaagta atttatcacc agtctggcat
     8761 gtcattgatt gttaatatca tctggctgat cgtttcaggc ctaccaattg ctctcgcaca
     8821 tgtcgtcagc ggcatccttc tatgtatcac gatcataggg ataccttttg ccaagcaatc
     8881 tttcaaactt gctcgattgg cgttgatgcc attcggtgca gaaattgtat aaaagagaat
     8941 aataaaggtt agataaagca tagagtcagg tgggatacat gtatttcacc tgactctatg
     9001 cgttctcctg tataagttct tccatgtgac ttttacgtct tttatttata tggggttact
     9061 gtttatggaa agttgaaata attttcatta aagaactctt tctatatttt tgaaagcgta
     9121 gtggagcttc gaatatctgg gaaggacatt ttgcagggat tgaaaaaaga tattgtccta
     9181 tcgaaaaatt aatgaattta gacaaattgc cagcaacagg cttcaaactt agttgctatc
     9241 ctgtcaagat caagaaggca agtgcgggtt ggataagagc agtggcaatg atcgaaaaga
     9301 aaaagaaaga ataagccaaa acatctgtat ggaagaaatt ttccgatgca gatgtttttc
     9361 attttttctt tcggatacct tgacatatga ataaccgttc atttataatt agttatgaag
     9421 agatattcat atgttgaggc gaggaggtca ttaaaatgaa aagttacaga ttagaaggtt
     9481 tagattgtgc gaactgtgca atgaaaatcg aaaaaggagt tcagaagatc aatggagtca
     9541 aggaagcaac ggtaaacttc acttctggaa aactaacgat tgacgcagag gaagatcatt
     9601 tggctacgat tgaacaagaa ataaaaaaag tagtgaagga attagaacca gatgtcaaag
     9661 taacagaaat agataaggaa aaagtatctg aacatggaaa tgaaaaagag agaaatacgc
     9721 tgttccggat tttattttcc cttgcaggta tcgctttatt gctattattt gattttaatg
     9781 aaccaattcg cttgataggg tatttattga tttatctgct gataggatat gatgtagtga
     9841 agaaggcagt gatgaatatc gtcaaaggaa aaatctttga tgagaatttt cttatgtctg
     9901 ttgctacgat tggcgctatg ttgatcggtg aatatccaga agccgtagca gtgatgctat
     9961 tttatcaaat cggcgagtat ttccaaggat tggcagtcag tcattcaaga aaatcgattc
    10021 gcgaattgat ggcgatccgt ccagaaacag ctcacgtgca gacgacggaa ggattgatga
    10081 ccgtgaaccc tgaagacgtg cagatcggtc agtttgtact tgtgaagcct ggtgaacgtg
    10141 taccgttaga tggaacgatc atcgaaggag aatcattagt cgatacgtca gcacttacag
    10201 gagaatcagt ccctaaatct gtatatgttg gtgaaacagt attaagtgga tttatcaata
    10261 aaaataaacc atttttagtt caagtagaaa aatcttatga aaattctact atcagcaaat
    10321 tattagaatt agtagaaaat gccagcagta aaaaagcacc agctgaaaat ttcattacta
    10381 aattcgcgcg ctattacaca ccagtcgttg taggattggc cgttttatta gctgttttac
    10441 ctccacttgt tgtttctgga gcggcgtttt ctgaatggat ctatcgagca ttgacatttt
    10501 tagtgatctc atgtccatgt gcattagtga tctccgtacc ccttagtttc tttggcggta
    10561 tcggaggagc aagtaaaatc ggtgtattag taaaaggttc aaattacttg gagttgcttg
    10621 cacaaactga gacagtggta ttcgataaaa ccggcacatt aacgaaaggt gacttttcga
    10681 tccagacgat cgatacaaaa atcgatccaa agatattcat gcaatatgtt gcgagtgcag
    10741 aacagttttc tacacatccg attgctcaat ctgttttaga aggctacgca ggtccgcttt
    10801 taccaacagc taatatccaa gaatttgcag gtgaagggat cttagctgaa gttgatggca
    10861 aacaagtatt agcaggtaat cacaaattga tggaaagatt tgaaatctct tttccttcaa
    10921 gtcaagaaat cggtacattg ctttatctag cgatcgatca aagttacagt ggttatcttg
    10981 ttattgcaga taccttgaaa gaagatgccg tagacgcatt ggttcaatta aaaaaggctg
    11041 gtgtgaagaa cacagtcatg ttgacgggcg attcgaaaaa aattgctgat catatcggga
    11101 agcaagtagg cgtggacaag atatatagcg aattgcttcc agaagataaa gtgcagcggt
    11161 tagaggaaat cttacaaaga aacaataaaa agaccgcatt tgtcggagat ggaatcaacg
    11221 atgcaccggt gctagcaaga gcagatgtag gaatcgcaat gggtggattg ggaagcgatg
    11281 ctgcaattga agcagcagat gtagtcatca tgaatgatca accatcgaag attgcagaag
    11341 caatccattt agcaaagaaa acattgaaaa tcgtcaaaca gaacattgtc tttgctattg
    11401 ggataaaaat tttggtactt gccttaggtg cttttggttt tgcatcaatg ggagatgcag
    11461 tatttgcaga tgttggggtg acagtattag ctgttttgaa tgccatgcga agcttgcatg
    11521 taaaaaataa acgttgatca atgaagagga cgaatgaacg tcctcttttt tttatatcct
    11581 tttcttaatg aaaaatgtaa agaaaataga taggattata aaataactaa tgtttttaat
    11641 tttgcagaaa attcaaaata gcttgaaatt caataaggta aatgaaataa aagaaaataa
    11701 cttatttttt tgatggaaaa aatatgaaaa aacgatgtgt attaaataaa agaatacaca
    11761 cataaaataa cactgtcttg taagtttgtt aaaaattgaa tatgattaca tctggataac
    11821 aaatttttat cataaaggag agaacgaatg aacaacaaac aattacgctg gtttactgtt
    11881 gcgttaattg catttaacat ggtttggggc atggggaatg tggttaataa ctatgctcag
    11941 caaggagtat cagttgtcac ctcttggcta ttgattttgg ccatttattt tattccatac
    12001 gcattgatcg ttggacaatt agggtcagcc ttcaaggata gtaaaggagg cgtcagttca
    12061 tgggtcgaaa atacgacatc taataaacga ctggcatact atgccgcttg gacttactgg
    12121 gtcgtccata ttccttattt ggcacaaaag cctcaagcaa tcctgattgc tggcggttgg
    12181 gcagtccaag gaaatggaaa tatcgtaaat acgatgtcag tacaaatggt agcagttatt
    12241 tcacttatta tatttcttgc ctttttatat ttatcaacaa aaggactttc tactttgaaa
    12301 gtgattggcg gactggccgg aacagcgatg ttcgtcatgt cgatcttatt tattctttta
    12361 gctgtaacgg caccgttcat caatccaacg atggaatttg ccacaccaca tatgaatcaa
    12421 gtgaaaacat atattccaac atttgatttc agttatttca caaccgtttc gatgctagta
    12481 tttgcagttg gaggagcgga aaaaatatcc ccttatgtca actctacaag aaatcctgca
    12541 aaggaatttc ctaaagggat gatcttctta gcagcaatgg tagggatttg tgctgtatta
    12601 ggctcagtcg caatgggtat gctgtttgct agcgataata ttcctaaaga cttgatggca
    12661 aatggggcat acagtgcttt tcaaatttta ggtaattact atggtatcgg taactcattg
    12721 atgatcattt acgcattgac aaacatgatt ggacaaatct ccgcattagc cttttcaatt
    12781 gatgcaccac taaaaatctt gttagcagat gcggatccag aatttgtacc aaactggcta
    12841 cgtaaacgta atcgtaaagg tacattggtg aatggttatc ttctgacagg tatactagta
    12901 ggaatcatta ttttgttgcc gatctttggt atcaaagata tgaatgaatt agtaaaatgg
    12961 ttgacgaact tgaactcagt tgtcatgcca atgcgttacc tatgggtgtt cttggcatat
    13021 atgatgttga ataaagctta taagaaattc aattctgaat ataaatttgt taaaaatcca
    13081 aaaatcggtt ttatctttgg tgcttggtgc tttgccttca ctgcgtttgc atgtatccta
    13141 ggaatggtgc caaaagtaga ttatactgct gatcctaaag catggtggtt ccaattattc
    13201 acgaatatca tcacaccaat tgtcttgatc ttattaggta tgatcttgcc agcaattgct
    13261 cgtcgagaca aaaacaaaga aatcactgaa ccagaaacag tggttcagcc ttaaaataga
    13321 aatacttgtt acaagaggtt gtgacagaag tggtcagctt caagaaataa gaaggaattt
    13381 acgaaaattg cttctcaaat ttttgtgaag tttcggcttg tttttcgcag aagctacttc
    13441 tggaacaccg tgaatcagag gttgtgaaaa agctgtcctg catcaagtaa taagaacagc
    13501 caaacaaaaa tagcccctca tatttttcgt tttgctgggc ttattaccgc agatgcaagc
    13561 ttttgaacac cgtttaagaa agaggtagtg agcctgccgt ttaactccga gtcacaagga
    13621 gcaatttcaa aaaacaactt ctcatgtttg ttgactattg tgccttgtga ccgaggtgtt
    13681 ggcaggagaa caccgtttaa ggaagaggtt gtaaaatcag cgatttgacc tgagtattag
    13741 aggaacaaac gaaaaaatag ctcctcatat tttgccgttt tttgagttaa tgctgaaggt
    13801 cagctgattg aacaccattt attcgaaaca agggactttg acacgacttt tgtcacagtc
    13861 ccttgttttt ttgcggcgtt ataagtggta tgatcgaacc aaattccaaa gagaagtgag
    13921 ggagaagaat gaagatcatc gtgattggac aaagtgggtc gggaaaatcg accctcgcaa
    13981 ggaagatcaa agaaatcacc gactttccgc tactgccgct agacctttta tggcatacaa
    14041 cagactacag tattcaggca aaaaagtggt ttttgcaaga acagcaatcg tttatggtat
    14101 ctaatccgtc atggattgtt gaaggaaatt atacttctac attagaggaa aggattaaag
    14161 aagcagataa gatcatttgg cttaaagtgc cgcgttatct cgccatctac cgagtagttt
    14221 ggcgttctct aaaaagaaag ataaacaaaa aatctcggcc agatatgcct gaatcatttt
    14281 cagagaaact aaacagggaa tacttagaat ttttatcttt tatctggcat tttgaagaaa
    14341 acaatgaacc gaaaatacag cagctgatcc aagtagctaa tgcaagaaat aaactgatca
    14401 tccttaaaac aagacgagag aaacaagaat ttttaactaa attgaaaata gagagcaaaa
    14461 ataattgaca gtttttgttt acaagcgtaa tctgtattca taagctaatg attacatttg
    14521 taaacgaaag gtgtgaaata aatggaaaaa gtcaaaacgc cagtaaagat cagtgattca
    14581 gagtgggaga tcatgcgtgt tatttggaca cttggacaga cgacagccca agaaatcacg
    14641 cagatcttag ctgattcgat ggattggaaa ccagcaacga tcaaaacttt gctgggacgt
    14701 ttggtaaaaa aagaagtgat ctggacagaa caagaaggaa aaaaattcat ttatcatcca
    14761 gctgtctcgg agatggaaac agttcgcagt gcgacggaaa atcttttttc ccatatctgt
    14821 gcaaaaagga ttggagagac gattgcggat ctagtagaag aagccacttt gactcaagaa
    14881 gatatcaatg agattttgaa acaactggaa caaaagcaac cagtagatac gatcgaatgt
    14941 aactgtatcc caggtcaatg tgaatgtaaa caacatcaat aggaggaaga aaaaatgaaa
    15001 caacaatttt caatcgaagg tatgagctgc aatcattgtg cagcaagagt agaagaaaca
    15061 gtaagcgcac ttgacggcgt gcaaaaagtg aaagtaaatt tgaaaaaagc aaacggtacg
    15121 gtcaagtttg atgaaacaaa agttcaatcc gaaaagattt gtcaagcaat caatggattg
    15181 ggttataaag cagaggtgat ttgatggcaa cagcagaaac caaaatggat acgttcgtca
    15241 ttacaggtat gacttgtgca aattgttctg ctaggatcga aaaagagctg aacggacaac
    15301 ctggtgtggt caatgccaca gtcaacttgg ccactgaaaa agcaagtgtc aaatatgaag
    15361 ggacgaccac tgaaaaatta atccaaagcg tagaaaacat cggttatggt gcaattttgt
    15421 acgatgaagc ccacaaacaa aaaattgcag aagaaaagca agcgtatttg aaaaaaatgc
    15481 tattcgatct tatattgagt acggttttga ctttgccgtt gatgttgtca atgattgcta
    15541 tgatgttagg aagccatgcg gcgattgttc acttttttca ttttccaatc gtccagttag
    15601 tgctgtctgc tcctgtccaa ttttatgtcg gtgctcgttt ttataaaggt gcgtaccatg
    15661 caatcaagac gaaagcacca aatatggatg tattggtagc aattggaaca tcggctgcct
    15721 ttgcattgag catttacaac ggatttttcc gtggacaccc ccaagatctt tattttgaaa
    15781 gcagcagtat gatcatcacg ctgattttgc tcggcaaata tttggaacat acagcaaaaa
    15841 cgaaaacagg taatgcaatc aaacaattga tgtctcttca aacaaaaacc gcccaagtga
    15901 taagaaacgg gaaagaagaa acgttagcga ttgaagaagt agtcgttggt gatcaactag
    15961 tcatccgtcc tggtgagcag attccagcag atggtcgaat tatttcagga agcagtgcga
    16021 tagatgaaag tatgcttact ggtgaaaatc ttcctgtaga aaagaatccg gatgacacgc
    16081 tttttggcgg gacaatcaat acgaatggtc tgctacacat ggaagtcaca caagtcggca
    16141 agcagacagt attagcgcaa atcattcaaa tggtggaaga tgctcaaggg agcaaagccc
    16201 caatccaaaa aatcgcagat cggatctcag ggatttttgt tccgatcgta ttagtcatcg
    16261 cgtttatcac gctcatagct acaggtttga tcactggtga ttggcaactc gcattgatcc
    16321 atagtgtctc tgtattagtc attgcgtgtc catgtgcatt aggtttagcg accccaacag
    16381 ctattatggt aggaacgggt gttggtgcac gaaatggaat tttaattaaa ggcggagaag
    16441 cattagaagc agctgctcac ttagacagca ttgttttgga taaaactgga acgattacag
    16501 aaggaaagcc caaagttaca gatctagtcg gttcaaaaga agtcctatct attttttata
    16561 ctttagaaca agcttctgaa catccactag gtaaagcaat cgtggaatac ggaaaactgc
    16621 aagaagcagc aacatacgat atgattgatt ttacagccca cccaggtgct gggatcagcg
    16681 gtacgattaa tggagtacgc tattttgcgg gtacacgtaa acgattaatt gaattgaacc
    16741 tatcatttga tgaataccaa gaacacgcgt tagaattaga acagcaggga aaaacagtca
    16801 tgtttttagc tgatgaaaag caagtgatcg ggtggattgc cgtggctgat caaatcaaac
    16861 tagaagccaa acaagcaatc aagcagctcc agaataaagg attagatgtt tttatgctga
    16921 caggagacaa caaactggca gccgaaacga ttggtaaaca agttggtatt gatccaaagc
    16981 atatctttgc tgaagtactt ccagaggata aagcagccta cgtagaaaaa cttcagaaag
    17041 atggcaaaaa agtcggtatg gcaggcgatg gaatcaatga tgcaccggcc ttagcattag
    17101 ctgatgtcgg tatggcaatg ggaagtggaa cggacattgc gatggaaaca gccgatgtga
    17161 cgctgatgaa tagcagtttg gcatccattg cacaaacaat cgagttgtct aggttgacat
    17221 tgagaaaaat caaacaaaat cttttctggg cttttgtcta taatacgatt ggtattcctt
    17281 ttgccgcact aggctttttg aatccaatca ttgcaggagg agcgatggcg tttagttccg
    17341 tcagtgtatt gttgaactcc ctgagcttaa atcgacacat gaaaaagtaa gagaagggat
    17401 ggagtaaaaa tgaatgggaa cgatatggag aaaaaacatg agcataaaaa agaagtagta
    17461 aacaaagaaa gtacagaatc tcatgaaaaa cagatggaga tgaaacatga ccatagtcag
    17521 atggatcatt ccatgcacat gggacataac catggagata tagcaaacag caaacaaatg
    17581 gaccattcga tgcacatgga tcacgaacac ggcggcatgg atcattccat gcacatgggg
    17641 aatttcaaac aaaaattttg gctatcgctt atcctagcta tcccaatcat tcttttttca
    17701 ccaatgatgg gaatggaatt tccatttcaa gtaacgtttc ctggttctga ttggctcgta
    17761 ttgatcttag caactatact atttatctat ggcggtcagc catttttaag tggagctaag
    17821 atggaattga aacaaaaaag tcctgctatg atgacactga tcgcgatggg gattacagtt
    17881 gcatatattt atagtgtcta ttcgtttatc gcaaatctgc tcaatccaca tacacacgtc
    17941 atggatttct tctgggaatt ggctactttg atcgttatta tgctactagg tcactggatt
    18001 gaaatgaatg ctgtgtcgaa tgccagcaat gctttacaaa aactagcaga attactgcca
    18061 gaatcagtga aacgcttgac gaaagatgga aaagaagaga cagtttcatt gaaagaagta
    18121 aacgaaggtg atcgtttgat cgtccggtct ggtgataaaa tgccaacaga tggtgtaatc
    18181 ttgaaaggtg aaacaattgt tgacgaatca gctgtgactg gcgaatcaag aggaatcaaa
    18241 aaacaagcca atgacaaagt gattggcggt tctatcaatg gggatggaac gatcgaaatc
    18301 gaagtaactg gaactggtga aaatggttat ctagcgaagg ttatggaaat ggtgcgaaaa
    18361 gcacaaggag aaaaatccaa gctagagtca ttatctgaca aagtggcaaa atggctgttt
    18421 tatgtcgcat taattgtggg tatcctggca ttcatcgcct ggttgttctt gacagatctt
    18481 cctaatgcat tagaacgtat ggtaacagtc tttatcatcg cctgtcctca tgctctaggt
    18541 ttagctatcc cattagttgt tgctcgctcc acatcaatag cagctaaaaa cggattactt
    18601 ttgaaaaacc gcaatgcact ggagcaagcc aatgatctag atgtgatcat gctggacaaa
    18661 acaggaacac taacagaagg aaaatttact gtaacgggtg tcgaagtact tgatgatgcg
    18721 ttcaataaaa atgaaatcct tcaatatcta ggagccttgg aagcgaatgc caatcatcca
    18781 ttggctgttg gcattatgaa ttatttgaaa gaacatgaaa tcaagcctta tcaagctgaa
    18841 aatctaaaaa atctttcagg agtcgggcta gaggcaacag tcaaaaaaca acaagtgaaa
    18901 atagttaatg aaaaagaagt tgagcgtctc ggattaaacg tagaacaagc attattgaaa
    18961 ccttaccaag aacaaggaaa taccatcagc ttcctgattt tagaaaatca tctagctgca
    19021 atcgttgctt taggtgacgt agtaaaaaca gaagcaaaag aatttatccg aacattgaaa
    19081 gaaagaaaaa tcactcctgt catgcttact ggtgacaata aaaacgctgc ccaagcagta
    19141 gcagattatt tagggatcga agaatattat ggcggtttat taccagatga taaagaagct
    19201 gttgtccaga aataccttga ccaagggaaa aaagtaatca tggtgggtga tgggatcaat
    19261 gatgcgccaa gtttagcccg tgcttcaatc gggatggcaa tcggtgcagg gacagatatc
    19321 gcaatcgatt ctgcggatgt cgtattgaca aacagcgatc caaaagatat tttgcatttt
    19381 cttgatctag ccaagcaaac gcgaaagaaa atgatccaga acctctggtg gggggcaggt
    19441 tacaatattc ttgcaattcc gctagcagcc ggaattcttg cgccaattgg tatcatatta
    19501 agtccagcag ttggtgctgt cttgatgtcg ttaagtacgg tagttgtagc tctaaatgct
    19561 ttgacattga aaatcaaata ataatgaaaa aaggggctct ttgtcaataa ggactgatgg
    19621 gttgtacaaa atcaaatctg ggtcagaa
//
LOCUS       DACDPS010000046        18426 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.046, whole genome shotgun sequence.
ACCESSION   DACDPS010000046 DACDPS010000000
VERSION     DACDPS010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.046"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(101..784)
                     /locus_tag="IWJ07_001738"
     CDS             complement(101..784)
                     /locus_tag="IWJ07_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP9333317.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(802..1419)
                     /locus_tag="IWJ07_001739"
     CDS             complement(802..1419)
                     /locus_tag="IWJ07_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAP9333318.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(1429..2253)
                     /locus_tag="IWJ07_001740"
     CDS             complement(1429..2253)
                     /locus_tag="IWJ07_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAP9333319.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(2246..2806)
                     /locus_tag="IWJ07_001741"
     CDS             complement(2246..2806)
                     /locus_tag="IWJ07_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333320.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            2980..3936
                     /locus_tag="IWJ07_001742"
     CDS             2980..3936
                     /locus_tag="IWJ07_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9333321.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(4241..5197)
                     /locus_tag="IWJ07_001743"
     CDS             complement(4241..5197)
                     /locus_tag="IWJ07_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAP9333322.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            5301..5903
                     /locus_tag="IWJ07_001744"
     CDS             5301..5903
                     /locus_tag="IWJ07_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9333323.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(5991..7490)
                     /locus_tag="IWJ07_001745"
     CDS             complement(5991..7490)
                     /locus_tag="IWJ07_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAP9333324.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(7506..8030)
                     /locus_tag="IWJ07_001746"
     CDS             complement(7506..8030)
                     /locus_tag="IWJ07_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333325.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            8328..9077
                     /locus_tag="IWJ07_001747"
     CDS             8328..9077
                     /locus_tag="IWJ07_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAP9333326.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            9068..9853
                     /locus_tag="IWJ07_001748"
     CDS             9068..9853
                     /locus_tag="IWJ07_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP9333327.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            9822..10538
                     /locus_tag="IWJ07_001749"
     CDS             9822..10538
                     /locus_tag="IWJ07_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP9333328.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(10640..11659)
                     /locus_tag="IWJ07_001750"
     CDS             complement(10640..11659)
                     /locus_tag="IWJ07_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAP9333329.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(11678..12736)
                     /locus_tag="IWJ07_001751"
     CDS             complement(11678..12736)
                     /locus_tag="IWJ07_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAP9333330.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            complement(12956..14104)
                     /gene="nagA"
                     /locus_tag="IWJ07_001752"
     CDS             complement(12956..14104)
                     /gene="nagA"
                     /locus_tag="IWJ07_001752"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAP9333331.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(14180..15016)
                     /locus_tag="IWJ07_001753"
     CDS             complement(14180..15016)
                     /locus_tag="IWJ07_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9333332.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(15200..16417)
                     /locus_tag="IWJ07_001754"
     CDS             complement(15200..16417)
                     /locus_tag="IWJ07_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9333333.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            16800..16883
                     /locus_tag="IWJ07_001755"
     tRNA            16800..16883
                     /locus_tag="IWJ07_001755"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16834..16836,aa:Leu,seq:gag)
     gene            complement(17123..18121)
                     /locus_tag="IWJ07_001756"
     CDS             complement(17123..18121)
                     /locus_tag="IWJ07_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333334.1"
                     /translation="MGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKIYWVS
                     IPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSLTTPGN
                     ALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLIFLLTYRKC
                     QNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLS
                     LFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVW
                     ASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         5500 a         3791 c         3188 g         5947 t
ORIGIN      
        1 aaaatttctc ttttttttga attctgaaca aatagtactg gagtacaaaa gacgaggaag
       61 agccactatc tgctcttcct cgtcttttga ttctcacctg ttattcatga ttctttttct
      121 cagtttcccg atatttttcc attttacgac taaaaagctc tccgctactt ggtggcgtat
      181 atgtgatggc atttgctcct gcttgaatgg tctcagcaat actttcttcc gttggaccac
      241 ctgtcgcaat gataggaagt tcaggatatt ttttacgaaa atggcgtact gcttctgcag
      301 tttctttacc tgcactgata ttgataatcg aaataccagc tgctaacttt tcatctattt
      361 ctgtatattt cgacgttacc gtactgataa ctggaatatc aactactcga caaacttctt
      421 ttactgtctc taaaggcgta ggaccgttca aaacgacccc taacgaacct tgcgcttctg
      481 cgaacatgct catgtaggaa gaacgagcac cttgtgtcaa accaccacct acaccggaga
      541 aaactggaat gtcagctgcc tcaataatgc ttttagtaat cgctggatga ggtgtaaaag
      601 gatacacagc aattactgca ttcgcatttg tattacgtat gatcgcaata tccgtagtaa
      661 agatgatcga acggatcaat tttccaaata tcttgatccc gcttgcttct aaaatgattt
      721 ccggtactct tacgatatct tttctcaatt ccgtcatgat ttctggcgta aattgttctg
      781 ccatcagcca cttctccttt gctatctccg gtcatactga ttcaattgat acgtctcgat
      841 gacctggatg attttttgcg cataaccggg gtctgtcgca taacctgcat cttgcaatgc
      901 ttgtgcggct tcctgatagt tggtggccgt aatcactttt tcatacagcg caggattcca
      961 atctactcct tggacgaaca gttgtgtatg gtcatccatg gactgttccc atgaatcata
     1021 cactttgaag tctccttgga tagttatcca ttcttcgtta acaaattcct ttgtttccag
     1081 gcttactttc ttctgatccc cataagcctt gatcccgaaa agattattgt atttgctagc
     1141 caaggtactt tttccccaat ttgattctaa tatagcttgt cctaaaatga tactaggaag
     1201 cactccataa ccatcttgta gttcttgggc atgcggaaca agccgttcaa taaattgctg
     1261 atgggattgc gcttcatttt cttgctgatt atcagtaaaa ggagaagaca gattcttcaa
     1321 ggaaaaaaca aaggctaaga gtatcactgc tacaccggca accactgctg gccaacggat
     1381 attcttgcgc ttttgctttt tccacttttt tttcgccatg atcaattcct acttttcatt
     1441 taattcattc agatattctt ctagcgtcaa ttggtgtttg gtgatatact cagcgttttc
     1501 tttaccgaca taacgtaagt gccaaggttc atatgtgatc ttagttatat cttcgtgtcc
     1561 ttttggataa cgaacgataa atccatactg cgcagcatta tctgcgatcc atttagctcc
     1621 aggctgatct ccgtaacttg catccaatac agtagaagga taactgttgt accagttttg
     1681 atcgacgaca tcgactgcca gacctgtgtg gtgttcgctg tatcctggct ccgtaatcgt
     1741 ttctttagta atctttgtcg cctcctcttc tgatacacct tgacgactca tcacatcttg
     1801 gacattttgt tcgaaaactt gttgttggga tgatacagac cggtaagctg agaccatcac
     1861 taatggaaat ccagctgctt cagcagcctt ggcaaatgct tcatattctg gagcaatgcg
     1921 tttgtcgatc aagtatccgt tacttaatga tgccaattga gtagactcat caatttcttt
     1981 ttctaatttg tgatctggac caaccaaaac tagcttccaa tcgtctgttt taacatctgg
     2041 caattgtttg gacgtacgat cttttgtttc tttgtccttt accttgctgc tggttgtttg
     2101 actgttcttt tctttactag cagttgtttt ggtgcttgtt cgttcgactg cttgattgcc
     2161 ggcttgaaca tgcgaatcat catcgctaaa aataacatag cccattgctg cgatcataat
     2221 cacaactgct ccaaatccta aaaatttatt cacttgaaaa atcctttcgt tgttcttcac
     2281 gttccccatt cgcctcatga acgtaagttt cttccagatt ttcattcacc caatcgagaa
     2341 gttcgatttt ttctccttcg atctgttttt gaacatctgc tggcagacga aaattcaaaa
     2401 tatcatcata gccccattca tcatattgaa tacttacttt caagtcaagg tatctaccag
     2461 cctgctgatt atttttcaaa gggatcagct cgacttttgc tgctttgctg ctaagccatt
     2521 tttgcgcgat gcgtgttttg atttgcttgt actctgcaac ggcaacgttt cgtttttcgg
     2581 gaaaaataat cgtttgacgc aaaatctttt gcgttagcat ccattcataa tcagtgaaaa
     2641 gattttttac tttctgcatc gcttcagatg gcagattttt ctcgccatct ttccattttt
     2701 gccattgttc tggactgatc agcaagtatt catctaaaaa atttttttca gtttcaaact
     2761 gttctaataa tacactcata ataagatcaa tatatacttg atgcacgttt atcccctcct
     2821 tttaactatt ctacttaagt tttgatcaaa aagcacctct taaatcgctg ctttcatttt
     2881 ttttaatgtt tcaagtcctc tcctctttat tcttcgtgat ttttgttata ataatagaca
     2941 gacaagaaac tcaatccaac tatgaaggag cgttttcaga tgaaaaaata tcaatgggga
     3001 atcgttggtc ttggtaccat cgcacatgag tttgccgaaa gcttcaatca agagacgagc
     3061 gaactaacag cagttgcttc cagaacttca gaaaaagcgg aaaacttcgc tcaccgttac
     3121 aatattccaa aagcttacgg aagctatcaa gaaatgttag acgatgctga aatcgacatc
     3181 gtgtacattg ctgtaccaaa tagacagcat atcgaccata tcttggcagc attggaagca
     3241 ggcaaacacg ttctatgtga aaaagccatc acgatgaata aaaaagaact ggctgacgct
     3301 atgcgcttag cagaagaaaa gaatttgatc ttagcagaag cgatgacaat cttcaacatg
     3361 ccactttatc agcagttacg ctcaataatg gatacaggaa aactgggggc tttaaaaatg
     3421 atccaagcac ctttcggcag ttacaaagaa ccagacccta aaaaccggtt cttcaatcca
     3481 gaattagctg gaggagctct gttggatatc ggtacgtatg ctgtatcttt cgctcgtttc
     3541 tttttgagct ctcaacctga agtagttgcc tctacaatgg ttccttttga aacaggcgtg
     3601 gacgaacaat ccgtcactat cttgcgtaac aaagaaaatg aacttgctgc tgtcagcttg
     3661 acttttcagg caaaaatgcc aaaagtcggt atcgttgcct ttgagaacgg ttatgtgacg
     3721 attgctgatt acccgcgtgc tgatcgtgcg gagatcttat ttacagatgg tacaaaggaa
     3781 ttcatcgaaa gcgggaatac atctgaagca atgaattacg aaataaagaa tatggtacac
     3841 atgattgaag gtgacttgcc caatcgctct ctcttcttga caaaagatgt catcgaaatc
     3901 ttggatcaaa tgcaagttct atggaaaaat atgtaatttt aaaaggttgt gaaaaagctg
     3961 ttctgcatca agtaataaga acagccaaac aaaaatagct cctcctattt ttcgtttggc
     4021 tggacttatt accgcagagg aaagcttttg aacaccgttt aagaaagagg ttgtgagcct
     4081 accgttcagc tccgagtcac aaggagcaat ttcaaaaaac agcttctcat gtttgttgaa
     4141 tattgtgact tgtgaccgag gagttgacag gagaacaccg tttaaagaag aggttgtgaa
     4201 atcagctttt tgacctgaat attagctgaa ttcacaacct ctatttttgg attttttgtt
     4261 gggagaattc tgccttgatt tcaataaaac ttttaaaaga ctgcaaaaac tggaacaatt
     4321 ctgctcgatc ctcgaattca gggcgactag tcggcagagg catttgccga tacaatacat
     4381 aaacttcttc aatacgtgtt aaaatttgtc tgccatcatt tgactctgaa aatgtttctg
     4441 cagtataaat caataaacca tagatatgtt ttccataata agctggttgc tggattctct
     4501 ctagattttc cagcatgtct ttaatcacat tggcctgtgc tcttctcata gagaagtaag
     4561 tttcgtagta tagcggctgt ctgagccagt agttttcttg atgttctctg gcatcttctt
     4621 ggctttcgcg aatgaatgtc agtaaatgtt cgcatttgat ctgcacatct tctttatttt
     4681 ctgataaaag aaattctgcc atttgtctca aaatatttcg aaattctttt tcgatctgta
     4741 tttggttttc cttcagctgc ttcgtacgat ctggcatgta aacatttgca agcaaagcaa
     4801 atactaatcc aatcgccatt aatcccgctt cattgccgat cagctgccaa gacatgtttt
     4861 cctccactaa ataatgagtg accaaaaccg aatttactac tatcccttcg gttaatttga
     4921 agcgagaggc aagcgggatg aacaggaata aaaaaagact gaagccaaaa atagtatacc
     4981 caacagcaaa aaaactgaac agtgctgttg ccgtagctaa tataaaggct gcgattcgat
     5041 tcatgcccgt acgcaatgta gagcgcttgg tatttcctac acttaataat gcaatgatcc
     5101 ctgcagctgg ccaatacaac agcgataagc tacccgctgt gaccatcgaa aagaagacac
     5161 tgatcactgt tttgactgtt cgcattccta tacgcattta cttccccctt ttttatttta
     5221 attatacttg aataatgaaa aactagctat cagacaaaag tttaggtatg atagtaaaaa
     5281 gaccaaaaag gagaatctct atgcaatttg gccgttttcg actaggtatg cgtacgatca
     5341 aaagtgcctt agctgttttt ttatgtatct tgttttttca tttgactcaa cgaggaatcc
     5401 cgatgattgc tgcattatct gctgtttttt ctttgcgtca ggacttgact acaaccgttt
     5461 cttttggaaa gtctaggatc atcggcaata cattaggtgg tacattagct atcatttatt
     5521 tttttgtcaa aagctatttt cacaatgatt ttttggtaga attgatcttg cttcctattc
     5581 tagttattat tgtgatcgtc gtgtcagatg gtattaacaa taattccggt atcatttccg
     5641 caatagctac gttgttgctg attgcgttga gcatccctca aggcgaatcc gctttgtatg
     5701 ctatccaacg tgtactagat acttttatag ggacattcat tgcaatcggt atcaatttct
     5761 ttcttcgtcc tcctgaaaca gaaaagaagg aagaactatc agaagatttg gtcgaactcc
     5821 aaaagaaaga acgattgcta agaacaaatt tacaagagat agaagctaag atccaaaaac
     5881 aaaagaaaaa tagcgaggac taattttctt ttgcaataaa ataaaaaaat ggagagaaga
     5941 actttaaaaa gtcttcctcc atttttgatt gaacggtgaa gtgtactact ttattcttcc
     6001 ttcatcgagt tttgttctgc ctcttttggc gcttcataca tatttacttg gctagtagtc
     6061 ccacgtgtcg acaaattctc tacgatttcc ttcaagtcaa gtcctgttgt cgcttttagt
     6121 gtttcttgag tagaagaaag caaatttgtg gcataatttg tcactcggtt cgctccactg
     6181 gtttctcctg aactgcttcc tgtatcgaca acagagattt tttcgatatt tcctagcggc
     6241 tgtgctgctt cgcgcatcaa ttgcggtagc atatcgataa ccatactcaa gacagctgct
     6301 tcgccatatt ccttgaaggc attggcgatc ttttgttttg cttcggcttc tgcttttcct
     6361 tttgctagtg ctgcttcggc ttctgcttgt ccagctaaac gtgtcttatt ggcttccgct
     6421 tctgccagtg cttccacacg gaatcgttct gcttcggctt cagtcacttc tttgactttt
     6481 tgagcttgtg cttcttgttc tttagcgtac cgatcggcat ctgctttttt cttcacttcc
     6541 gaatcatatt gcttttcacg acgtgtgatt tctttttctt ccagttcgat ttgtttttgg
     6601 cgttctacga ttttaatctg catttcctgt tcgattactt gttgttgtgc tcgggcactt
     6661 tctaaattgt aggcttggtc tgcttttgct ttagcaacat cttgttcttg tttgtaggtt
     6721 gctaatttca gttctttttc ttttaatgat tctgcgatct ctgtttgtcg ctgtaattca
     6781 gctgcttgtg attctttttc tgcttgagct tttttgatac gcgtttcttt tagagcttcc
     6841 gcttctgcta tatctgcatc tcttttgact tgggcgattc ttggtttccc taaagagtca
     6901 agataaccgt ttttgtctct gacctctttg atagtgaaag aaacgatgat caaccccatt
     6961 tttgcaaggt ctacagaagc gacttcttgt acgctttgac tgaacttatc gcgattttga
     7021 tagatttctt ctacagtcat agaaccaaga atcgagcgca agtgtccttc taatacttct
     7081 cttgcttcat tttccagttc ttctcttgtc ttgcctaaaa actgttctgc tgctgtagca
     7141 atctcttcta cagatgaacc gattttgatg atcgaagtac catcacacat caccggtacg
     7201 ccttgttccg tatacacttc aggggtggag acgtccaatt tactcgataa tagactgatc
     7261 cgattcgaac gttgaaaaac tggcaagacg aatgcgccac ctccacgcac aattttgatt
     7321 ttgttattgc tttcatcaat gtgcacattt ttgcttccta aataacttcc actgatgatc
     7381 aacgcttcat ccggttttgc tgtctggtac ttcgctacaa agacgatcag caacatcagt
     7441 aaaatcaaag ctgcgattgc gatcgcgata aaaacaactg gtatttccat ttaacacacg
     7501 ctcctttaag cttttatcat gctttcataa ggcatgacat aacagattct ttccttgatc
     7561 tcaatgatca atactttcgt tccccgctca gctgtctgtt cttgggggct gtaaaaacaa
     7621 gctggacgac taatggaacc ggtaacactt ttcaactgaa tttctcccat acctcgtaca
     7681 gggatcggtg tgatcacaac agctacttgt ccttccagag ttttttcaga agacgacatc
     7741 gttgattcgg catgtttcat cggcatcaat atatagaaat taagcaaaaa taccaacact
     7801 atcgatatgc ctccactagc aataaataaa aataaagatg attgatctgt taaacgctca
     7861 cctagaaaac caaataagct tgtgaaagtt aaccaaggga taataagcat cggatcaagc
     7921 gggccatcaa aatcgaatac atctccaaaa ataatcaata ggactgccaa tcctgcacaa
     7981 atcagcaatg tataccaata aatcgtctca agtgcgattc cccctatcat atacacgcct
     8041 ccttccattt ctacgtctat cataccaatg tttgaaaatt ctgtatagct tcttttaccg
     8101 actcagtggt gttttttata ggaaggttgt gtttatcgtt gacaatttgt taaatttttg
     8161 ttttcatgtt aaaattttca caataaattc tttcgaggta gcctatgttt ccttttttta
     8221 agaaaaaacc attaaagctc cagaacacga tcatccttgt cgtttgtttc tccacattcg
     8281 tttctttgat tgtttctgcc atattgatca ggaattttat tctttcgatg aaccgatcta
     8341 gtgtgtcaga taaaatctct agcattgcta aaatcgttgc caatgataaa ctcatccaaa
     8401 aaaagctgca gactggtgac ccgacacatg cgattcaagg aataacaaat gaaatcatga
     8461 ttgcatcgaa tttagatttt atcgttgtca tggaccctga aggcattcga tattctcacg
     8521 cagacccaag ttacatcggt cttcctttct caagtcatga agatgtgaaa aaagtgattg
     8581 gaggacaaga atttttctct gaaaaagaag gaatgttagg gttcggcttc cgctatttcg
     8641 tccctattta ttatgatcaa gagattattg gtgcagtttg tgttggctta acaaacaaca
     8701 cgattgacaa tcaagtcaaa gatggacaaa aaatgattgt tttttcattg attattggac
     8761 ttagcgtagg catcataggc gccgtttttc ttgcccaaaa aatgaaaaaa gtactgcttg
     8821 gacttgaacc acaagaaata gcagaacagt taagtgaaaa aacgattatc gaaaatgaag
     8881 tagatgaagg aatcttagcc atctcttctg ataaaactat cctcttgatc aatacacctg
     8941 caaaaaacat tttctaccaa acagagcagc tattggattt cgaagtagga aaaaaatcag
     9001 tgataatcta tatgacgttt tgtttaaaga gacgattgag catcaaacga ccgttcggac
     9061 tcaatccatg tatttaaatg ggatagaaac tatttctagc gttacacctc tttttagcaa
     9121 tgaccgtttt ttaggagcag tcgcaacttt acgaaatcga tcagaagtaa tgaaattaat
     9181 ggaggagctt ggggggacga aacaatatat aaactccatg agagctcaaa cccatgaatt
     9241 catgaataaa cttcatgtca tttctggttt gattgatttg aagaagtatg aagaagtcac
     9301 ccattttatc cagcagctaa atcaaaatta taaaaaagag attggccaag tctcaagtct
     9361 gatcaaagta cctgcaattg ccggtttttt attaggcaaa ataaacgaag ccaaagaaca
     9421 atctgttgaa atgctgttag aaagcgattc ttttatccct aaaatggaga tggatgactc
     9481 catccattca cttttgcaag tgttagggaa cttacttgat aatgcaaaag aagccgtgct
     9541 acaaaaacat gaaagaaacg gacaggtccg gattcattta aactatgaca cagaagctga
     9601 agtattcatc gtgaccgtct gcgatggcgg tgtcgggata tcagaagaaa tctcagaaaa
     9661 aatatttacg ctaggttttt cgacgaaggg aatcaatcgt gggtttggat tgaatctcat
     9721 ccattcaatc gtcaaacgcc acggtggatt tttagaattt tccaaacaaa caaatggcgg
     9781 tacaatcgtc tatctagaat taccaaaaaa ggaagatcaa aatgatgaca aatgtcctga
     9841 taatcgaaga tgatccaatg gttgcagaat tgaatcgtac ttttgtggaa agcatcccta
     9901 atatgaacgt aatcgaaagt ttcagaaatg tagaagaagg aaaaaaatat ttcagacaaa
     9961 aaaacgcaga tctgctgctg cttgatgtat acatgcctgg cgaaagtggt atccattttt
    10021 tatcttggct tagacagcaa ggaaatgata tcccagtgat tttgatcact gctgcagatg
    10081 atatcgaaac cataaagaat gtgatccgct acggtgcctt agattatttg atcaaaccgt
    10141 tcactttgca acgttttcag ctggcgatcg aaaagtatct tgctttctct cagacaatcc
    10201 aaacgactga aaaagccgat cagaaattga tcgatcaagt attcaaccac accactccca
    10261 ttcacgaaat accatcattc atcgatttgc ctaaaggtct gtcaaaactg actttaaaaa
    10321 aaataagtgc atttattcta aaagaagata ctattttttc aacaaaagaa ctggctcaaa
    10381 aaagctcact gtcacgtata tccgtcaaga aatatttgaa ttttttagta gaacaagata
    10441 tattgaacga aagaatcaaa tatttggaaa tcggccagcc gctaacctgt tatgaattga
    10501 caaagaatgg gcggaaaaag ctcacagatt actgttgagt catcgaatta tcccatacaa
    10561 aatagtccaa gtatacagaa gtaacaactg tttacttgga ctatttttaa acattttatt
    10621 gacgaatatc catgttttct tatcttaatt gacgggcttt aggaagagat acctcatttt
    10681 tcgtttttcc agtttctatt atttcacgaa agttctcgaa acttgtcgaa atcatatttg
    10741 ataatgcctc atctgtattt gaacctacat gcggggttac aagaactcta gggtacattt
    10801 ctactaattg ctggatagca gggtctggaa tcgtctccca tggcttgaag gatcggaaaa
    10861 aaacctctgt ttcatttgca aaaacatcgg ttgcaaaacc agctaaatga ttgcttttca
    10921 aagctcttaa aatagcttca ttatcttgaa gctctccacg ggctgtatta atcaagatgc
    10981 tacctttttt catttgagct aaaaatcctt cattgatcat ttgatcgttt tcacctggga
    11041 tatgaggtac atgaatactg acaatatcgc ttttggctaa taactcttca agcgagtgga
    11101 acgtcaatac ttttttagca gcttctgtct gatgaatatc gtagccgatc actttggctc
    11161 ctagtccctt aaacaaaaca gcttccgtca accctatcct gcctgttcct atgatcccta
    11221 ctgtgcaatt tctgatttca cgactaaaca tttgctcatc cactcgaaaa tctttgaaag
    11281 atgttcggat cgtcgtatac gcagtatttc tcaaaagcat catggcaaaa gtcaaagata
    11341 actctgctat cgcatttggc gaatacgatg gtacacgggc tactgtcata ccaaaatcag
    11401 ctgccgcttg tagatcgata tgattgactc ccacagtccg tgtaaaaaca taacgaatcc
    11461 catattcctg catttttgca agattggtct catctgctat acaatttccc ctcaacaata
    11521 cagcatcatg gtcctttgct gacacgatat tgtcatgtgt caacagttct tcataaagac
    11581 tgagatcata atggtaaata ttcaattttt caaaaaaatc tatctcgttc ggacgaacac
    11641 cgtaacatgc gattctcata ctttcttcct ccatttctta tgaaaataaa cctatgccat
    11701 gaatcatttg taatacaacg atccatacag gtgtcagtac cacagcaaaa atagttgaca
    11761 gtaaggatgc attggaggct aaaagtgcct ccctatcaaa actgatagca taggctgctg
    11821 ccactgttgc agctggtgta gccatcatga taacgattgt tgccagtgct tcaaaattga
    11881 ctggtagaat attcgttgcc gtcaaaactg ctagaatcac gatattcaat gcaggtacaa
    11941 ataccacttt gactagactg taataccatg aagttttttc agatgcagct tttttgaagc
    12001 ttacttcacc taatgttgcg ccgattgcta gccacgccaa tggtgaactt aaaccagcta
    12061 aataatccat cggctgaaat agccacggtg cagtctggtc aattcgcaaa aacgctactt
    12121 ggcttatctc tccttctgca cctgtaactg acacttgtgg cagataccct tggaacagcc
    12181 aaatgaaaag gccggcaaaa gtagcgataa tgattggatt gacgaacatc gctttgatat
    12241 ttttagcttc catcttcaag ccactcattt ttatgtatcc ataagaatac aaaaagattc
    12301 ggtagccaat attgaaaatt gaggaataca tcacgccgac aggaccataa atcgcactga
    12361 cgataggcgt tccgaaaaag gtcgtagaac caaagatcgt caggacacgc aacgttgttt
    12421 gcttatcccc tttatagctt aagaaaaaag gttttgaaac tagaataaga ataatataaa
    12481 taactaatcc ccaaatcaag acattgattc cttgcatcag taattctgta ttgatatctt
    12541 gcatgaaagc tcgaaatgcc agtgctggca aagccactgt taagacgact ttcgatagaa
    12601 tttttccaac agattcagaa aaaatcccct tttttctaca aacaaaccct aataatataa
    12661 taaaaactgt tgaagtgatt gcactgacaa tcgacatatc tgtcaaagtc gatcgaaaaa
    12721 tgtcaaataa gttcatcttt ctcctcctaa ttatgttcat ttattcacaa taaaaaaagc
    12781 ttttattttg ctaatacgta ttaacatttt taattttatc tttacattgt tacttattga
    12841 acaataggaa attaaagaaa cttttgaaac ttatgtaaag tgatttttac tagaacacaa
    12901 aaaaacctta aaaccaaact taagaagttt agttttaagg ttaacttaaa ttatattatg
    12961 cgtggaaacg ttccacgcca tctagataag ttgcttccaa tgtcatatca gggttcaata
    13021 cgataaagtc tgcatcgcgt ccttgagcga tttttccaca tgtatcatcg attttacagc
    13081 tgattgctgg aatcagacta gccatcatga ctgcttgttc tggtgttgct aatccccagt
    13141 ctacaacatt tttaattgct tctttcaatt tcaatatact tccagctaag ttgccagatt
    13201 ctagacgtgc tgttccttct tttactacta ctggaaattc gcctaaattg taatttccat
    13261 caggcatccc accggccatc atacagtctg taattaatgc gacatgatca tgtcctgctt
    13321 tttccattag gattcccgca gcatttggat gtacatgatg gccatcacaa atcaattcag
    13381 aaaagacatg ttctaaagat aataacgcac ccaccatacc tggttcgcga tgattcaatc
    13441 cacgcatacc attgaaagca tggacaaata cacttgcgcc tgcttcaaca gctttttgcg
    13501 cttcttctag tgtagcatta ctgtgtccta gagaaacaac tactccttct tcagtcactt
    13561 gtttgacaaa ctcttctaca ccttgacgtt ccggtgctaa agcaattttt ttgattaaac
    13621 cacctgaagc ttcttgccat tcatggaaag tatccaaatc aggatccccg aaataacttg
    13681 ggttttgtgc ccctttgtgt tcttctgtga agaaaggacc ttcaaaatag attcctttga
    13741 ttttagctcc tggtgcttcc tgatacattt tgccgattgt ctcagctaca tcttttaaac
    13801 gttctttgct tgacgtcaat gttgttggta aaaatgaagt cactccacaa gataacagac
    13861 cttcagacat cactttgatg ccttctgcat cattatccat tacatcatga ttcatatagc
    13921 cgtgaatatg tgtatctacc aaaccaggag caatccattt gccgctttgg tcaatgatgt
    13981 tgacttctcc ctcaggtagt tctttggtgt attcaccaaa aagtccatcg gtaatttcaa
    14041 gatagcctgc atgtttgaca cctgacgcta aaaagaattt gtcagcatag ataaaagttc
    14101 tcattctatt ctctcccttt gtttttctac ctctaaagta ctcttattta gagaagaagt
    14161 caagaatggt ctacaccatt tacaaatttt ttgaaatttg tttcacagct tgtgctaagc
    14221 cttcatagaa tgctgctctt tctggttctt ctttacgctt gtatattaag cttttaagca
    14281 caaataaatc attgaggtaa tagtagctat tcttctcttg agcccaatag attccttgat
    14341 tcacctgttc taacgcttcg tcgatttcat tttgatggaa taagaaattt ccataattat
    14401 aaaaaatttg cgaaaattca ctttttgatc gttcatcagc tgattgatgc agcagttgga
    14461 tgcttcgact caaatagtag cgtgcttcag ccgtttttcc tgccacacca tatatttttc
    14521 cgatacaact aatcagttgt atttctgtgt cagaaacatg gattctttct ttttggctag
    14581 agtagcttaa tgcttctttc aaataataaa gggactgaga gatattttga cgcaacgtga
    14641 attcgcaaac accgagataa taataatagc gttgaaagtc agttgcttga tacagatttt
    14701 cagaaatatc agaagatttt agtacttgtt ctaacttccg ataatcacgt ttttggaaac
    14761 aactatccaa cagttcaaat aatttatcgt ttgtccgagt agtttttcga gagagattca
    14821 tcacatgatc aatcgttacc cctagccgtt gacaaatctg catcatcacc aatacattcg
    14881 gcagttcctc attattttcg atccgactta atacactttg tgaacaaata tcatgcgaaa
    14941 gcattttttg cgtcattttt tgttcttttc gaatctcttt aatgatcgtt ccaaaggact
    15001 cgattaacgt ttccataagt atcctcccca aaatatgcgt attcgcattc tattcgtatt
    15061 ttactttagg aagtcgcaaa cttcaagaaa agacttgcaa ctaaataata tactttagtt
    15121 atttaagaaa actccactag aaagagagcc tcagtgtgtt tgtctgtggc cctcctactg
    15181 aaaaatttgg tttttaaaat catttttggc gataggttgc taggaattct ctcattttct
    15241 tagctgtttg tttcaaatca tccattttag gaagataaac gattcgaaaa tgatctggct
    15301 ggttccaatt aaatccacca ccatgtacaa gtagaatatg atgttcatgt aaaaagtcca
    15361 aaacgaattt ttcatcatcc aaaatattga atcgctttgt atcaattttt gggaatatat
    15421 aaaaagcagc ttttggtttt acggctgaaa gaccaggaat atcattgatt gcattataga
    15481 tatattctct ttgttcatag atccttccgc ctggcaggag cagctcatcg acactttgat
    15541 aaccgcctag cgcggtttgg ataatttgct gagacaatac attggaacac aaacgcatgg
    15601 aggacaacat attcagccct tctatatagc ctttgacgtg tgacttatct ccacttagga
    15661 ccatccagcc tactcgaaat cctgctacac gatgagattt ggatagaccg ttcagcgtaa
    15721 caacgaacaa atcaggcgct aatgtagcaa tcggaatatg ttctagacca tccatcacta
    15781 agcgatcata gatttcgtcg gaataaataa tcaggttatt ttgtcgagct acttcaacga
    15841 tttcttctaa taattctttt ggataaagtg ccccagtagg attattagga ttaatgataa
    15901 caatagcttt tgtccgactc gttatttttg atttgatatc atcaatatca ggataccatt
    15961 ctgcctcttc atcgcaaata taatgaacag gtgtgcctcc agctaatgca atagaagccg
    16021 tccacaacgg atagtccggc attggaacca gtacttcatc tccattgtcc agcaacccct
    16081 gcatacacat cgtgatcaat tcgctcactc cgttgccggt gtagatatca ttgatcgtca
    16141 cattcggaaa atgtttcagt tggcaatatt gctcaattgc ttttctagca gaaaagatcc
    16201 ccttggaatc tgagtagcct tctgagtcac gggcattcat gatcaaatca cgaatgactt
    16261 cgtttggtgc ttcaaatcca aaaggtgcgg gatttcccgt gttcaatttt aaaatactaa
    16321 tgccctcttc ttgcatccgc tccgcttctt ctagaacggg accgcgaaca tcgtaactga
    16381 caccttctaa tttgttggat ttttcaaatt ctctcatcac ttagccaatc cctcatttca
    16441 aaaagttaga actttccaaa ttttttgtta tttactattt caatatcata ctccatccta
    16501 gaaaattttg ccacttattt aggaaaaacg taagtattaa aaaaacacaa acaagcgttg
    16561 agaacttaat tgtcatgata gtcattccat agattcgctg cccaatccat tgcaattcca
    16621 caaatgattc ctagcataaa accaagcaaa aaacgtccga atgcaagtcc aatcaataca
    16681 cctaagatca ttcccagtcc tgaaaattct ccgtcatatt tctccatctt aataagtcct
    16741 cccatttttg agcttgacaa ctatgtaaaa cctcgctatc attagtgtgt tattattacg
    16801 cagatgtgtt ggaattggca gacaagcatg attgagggtc atgtgagcgt tagctcgtgt
    16861 gggttcgagt cccatcatct gcactacaac tcgtgaagtt aattttgctt cacgagtttt
    16921 tttgtttgaa cgtttcttta tgccattata tacatatcac tgtatccttt acaaaaagaa
    16981 cgtacaatca tgtaaacagc ctgaggtgag tatatgaagg tcggaaacaa gctactcgta
    17041 gaaacttgca agtgttaaaa caattaatcg aaaaacgagt aaaatccctc acctaatact
    17101 aaagcgaggg attttttgta tattatctgt ttatcaccgt tattataaag gtacaaatac
    17161 caagcacggt taatccaaat tgcataaatt catttttttg tacactcatg cttttcatta
    17221 gctctttaac tgccattagt actaagatgg cgataacaac ataaagtata tagtacgtcc
    17281 gcattccgga agcccatact gtgggagaaa atcccataat taaccttgaa caaaatccca
    17341 ttatcatcaa aataactaaa aaaatagaag ttttcttatt gtcaaaactt aaataaattc
    17401 caacaattaa acatagtaat aatgccaaga ctactaaagt agctatccaa gtacctggat
    17461 aaaaaagtga cagtttcgtt cctaactctg taaagagatt attcaggtta ctactactcc
    17521 aaatcattgc ccttttattt ccattgacgt aagtaaagct ttgacccatt gtattaccaa
    17581 aataaataat caggtttaag aaaaacggta atgcagtcaa tatacgcaca taatagtttt
    17641 gacatttcct atatgttaga aggaaaatta agaaaaatag caataaaaat aagatattca
    17701 tgtctaaaaa aaacggcttt ccaaatgatg aaatacctaa atctaactta ttaagaaagt
    17761 tgaaattttt atattcagga aaccatttat taatttcatg tgcagcacgt aatgcattcc
    17821 ctggagttgt caaagagaaa attaattctg ctaagcttat tattgaaaat actgataact
    17881 tataattata tcgtccattg acaatcaaat ataaactaac tatacttgtt agaacaaaaa
    17941 aacaggcgtt gacttgttct tgattagtta aaaatatcaa taaaggaata cttacccaat
    18001 atatttttct atttatttct tctcctctta agagttgaaa aaacggataa aaagttagta
    18061 aactaaatgc tactggccac tgatagttca aggtggttgc tctccaaccg gtttccccca
    18121 ttactgtcaa tggtattaaa cagtatatag agcaaaccaa taataaattt ttaacattta
    18181 ttttctcaaa aacatacttg caaagtaata ctacaattcc taacataatc gtagaattta
    18241 ataatttcca tagcataaag tgtttagaaa agaacattaa gaatagttct ataataattc
    18301 ttgaactcca tgtcttataa tgttctatcg aagcacccaa atagccaagt tcatctaatc
    18361 tacttgcata aactacgtca tccgctctca tagtcggcat atacatatta aagagtaaaa
    18421 agatac
//
LOCUS       DACDPS010000047        18277 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.047, whole genome shotgun sequence.
ACCESSION   DACDPS010000047 DACDPS010000000
VERSION     DACDPS010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.047"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(105..560)
                     /locus_tag="IWJ07_001757"
     CDS             complement(105..560)
                     /locus_tag="IWJ07_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333335.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(714..1922)
                     /locus_tag="IWJ07_001758"
     CDS             complement(714..1922)
                     /locus_tag="IWJ07_001758"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAP9333336.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2034..2366)
                     /locus_tag="IWJ07_001759"
     CDS             complement(2034..2366)
                     /locus_tag="IWJ07_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP9333337.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2514..3389
                     /locus_tag="IWJ07_001760"
     CDS             2514..3389
                     /locus_tag="IWJ07_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9333338.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3471..4052)
                     /locus_tag="IWJ07_001761"
     CDS             complement(3471..4052)
                     /locus_tag="IWJ07_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAP9333339.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4058..5317)
                     /locus_tag="IWJ07_001762"
     CDS             complement(4058..5317)
                     /locus_tag="IWJ07_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP9333340.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5319..6365)
                     /locus_tag="IWJ07_001763"
     CDS             complement(5319..6365)
                     /locus_tag="IWJ07_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333341.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6600..6875)
                     /locus_tag="IWJ07_001764"
     CDS             complement(6600..6875)
                     /locus_tag="IWJ07_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAP9333342.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7118..8428)
                     /locus_tag="IWJ07_001765"
     CDS             complement(7118..8428)
                     /locus_tag="IWJ07_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAP9333343.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8610..9839)
                     /gene="rpsA"
                     /locus_tag="IWJ07_001766"
     CDS             complement(8610..9839)
                     /gene="rpsA"
                     /locus_tag="IWJ07_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAP9333344.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9974..10654)
                     /locus_tag="IWJ07_001767"
     CDS             complement(9974..10654)
                     /locus_tag="IWJ07_001767"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAP9333345.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10703..11338)
                     /locus_tag="IWJ07_001768"
     CDS             complement(10703..11338)
                     /locus_tag="IWJ07_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9333346.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11404..12807)
                     /locus_tag="IWJ07_001769"
     CDS             complement(11404..12807)
                     /locus_tag="IWJ07_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAP9333347.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12794..13825)
                     /locus_tag="IWJ07_001770"
     CDS             complement(12794..13825)
                     /locus_tag="IWJ07_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333348.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13856..14077
                     /locus_tag="IWJ07_001771"
     CDS             13856..14077
                     /locus_tag="IWJ07_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAP9333349.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14242..14979)
                     /locus_tag="IWJ07_001772"
     CDS             complement(14242..14979)
                     /locus_tag="IWJ07_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAP9333350.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15132..16472)
                     /locus_tag="IWJ07_001773"
     CDS             complement(15132..16472)
                     /locus_tag="IWJ07_001773"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAP9333351.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16709..18232)
                     /locus_tag="IWJ07_001774"
     CDS             complement(16709..18232)
                     /locus_tag="IWJ07_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAP9333352.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
BASE COUNT         5144 a         3901 c         3097 g         6135 t
ORIGIN      
        1 cttgtcacag tcccttattc tgaataaacg gtgtggaaga gacagcctat cagtagtaat
       61 aagacacatc tctttcacag cctcattttt gcgtaaaaat cgatttattc gaaaaattcc
      121 ccgtatacat cttctacacg ttgatctgga ttgatcacga tatacaagtt acctgttttt
      181 tcgctgattt ttgttgattg atattgattg tctaatcctt ctgttccttt atctagatat
      241 gggaagtaca caagatcagg tgtgccattt cggtaaagga tttccattcg ctcgatatct
      301 tcgtatttca atgcgcgatt aaacatgccc atttccagtc cgcctaggtt gatatcatgt
      361 gtcgcaactg cgtccccttc atggaagatt tcgattttaa atccagcaca cggatggatt
      421 tcaacaaatt cactgccatt gattcgtcca aaacttgttg tgatttgttt gatccaaaga
      481 tcgccgatat gacggcggtt gatcgtccag gtttctccat ttctaaaata aaattttagc
      541 gctgtcattt ctttactcat taaaaagact cctttatgat gtaatttatt cttgtactac
      601 ctcagtttag cacaaattta tgtaagtggt cacatcttat gttttttctt ctaatgacgt
      661 atctgcccac tttaagtagc caacaaagct gagcagatac agtctagtgt tttttaaatc
      721 aatttttctt tcgataaaaa atccaaaacc tgttttttat cattttttat tctttgttct
      781 acgacggctt tttcagctaa ttgcaaagct tcactaaccc acttaccagg tttttttcca
      841 gtttcttcta gcaagatttt accatctact gctaattcgc ttaatgactt gataggtaaa
      901 cgatcatagg attctcctac ggcatccatc tgaggtgttt cgtcaaaata gatcatcaat
      961 tcttctactt gcagtgcatg ttttttgcct aagaagtaca attcgaatgg ttccaaagct
     1021 ccagtttctc gttgtcgtag tccttggaca agtgcttgaa cgtctttaat catttggtta
     1081 gaacacttcc acgatttcaa aaaggaacga atctcatttt cttttagatc cagttggctg
     1141 atcaataatg cccatgcttg gctctcttct ttcaatggga cggatggtaa ttcagcaaaa
     1201 cgtaaaagtg cttcgccttc ttctttcaaa ccgggacagc aaatgtagca ctctgtttca
     1261 ataaatgcat ggattcccgc ggcccgttca tttcccaaga gcatcttgat gaattccaca
     1321 tttatacgtt ctacagatat tttacctaat agctcgtggt tttctttgat ggcgtagaag
     1381 gtttcttcct ctaatttgaa ccctagttga ctgacaaaac gcaacccgcg catcattcgt
     1441 aaggcatctt catggaaccg ctcatgagga ttgcctacag ctcgcaagat ctgttcttct
     1501 agatcttgca gaccatcgaa caaatcgatg atttctccat cttctttcaa agcaaacgca
     1561 ttgatcgtaa aatctcttct tttcaaatct tctgccaacg aacgaacgaa ttcgacatga
     1621 tctggtctac ggtagtcctg ataagttgat tccgtccgaa atgtggtgat ctcatattgt
     1681 tcatcatttt ctaataccaa cacagttcca tgttcgatcc caatatctat cgtttttgga
     1741 aaaagctgtt tgatttcttc tggaaatgcg cttgttgcga tatccacatc atggattggc
     1801 tggttcagca agatatcacg aacacttcca cctacaaaat aagcttcata tcctgcattc
     1861 tctaattttt tcagaactgg aattgccttt tggtattcca gaggtaattg ttttaatttc
     1921 atttttccaa cctttcctgc gtgtttcttg tcctgagata aagactcagt tgcatggtga
     1981 ctgtttgaca agtccgtttc agagtcttca aggccaggac cacttcatct tgtttactcg
     2041 tccttacgct caaagcgatt atggtctctt tgattgaatt tttccatgtt tttttcaaaa
     2101 acttcagtta agtcgatatt caatgaatta gccatgatca tcgtaacgaa taaaacatca
     2161 ccaagttctt ctgctaccga gttaactggt tcagaagcct ttttgctttt ttctccataa
     2221 gtgtggttga cttctcttgc caattcacct acttcttcag ttaatcgagc catctgagct
     2281 aatggagaga aataccccac cttgaattgc tggatatatt catccacttc ctgctgcata
     2341 ctgtataaag aacgcttttt atccatcttt ttccctccaa aactatctta ccaaaacttt
     2401 aacgcaaatc ctagataaga gcaattgaga aaatcgaatt tccatgctac atttaggaag
     2461 gaatttctaa atggaaattc tgtgaaaata cataattgaa agctggaatt catatggtca
     2521 aacgaaaaac agtaaaagaa gttcttttta tcattatagg aacaagtatt tatgcatttg
     2581 gtcttgttta cttgaacatc gctaatcatt tagcagaagg gggagtctca gggatcacct
     2641 tgatcttaag agctttattt ggtattgatc cagcttatac gactttatta atcaacatcc
     2701 cattgatttt actaggagga aagatcctcg gtaaacgttc cttggcctat actgttctag
     2761 gtacactttc tttgtcattc tttttatgga tctggcaaag ggttccacta gaaatcaatt
     2821 tacagcatga tctactgatc gtttctctac ttgcgggttt agttgcaggt gtaggaagcg
     2881 ggatcgtcta ccgcatgggt ggaacaacag gcggcagtga tattatcgct cggattttag
     2941 aacaaaatta cggcatcact atggggcgtt ccctattggc atttgatatc atcgtcttac
     3001 ttgcttcatt aacctatatc gacttgaaac gtatgatgta tacgttgatt gtttcgtatg
     3061 ttttcagtcg tgtgattgat tcgatcttag atggcggata ttctgcaaaa ggtctacttg
     3121 tcgtctccaa caaaagcgaa gagattgcac cactactgat gaccggtctg caaagaggtg
     3181 tgactttcct ttctggagaa ggcgcattct ctggtaattc aaaaaaaatc atctatatgg
     3241 tcgttagctc tagtgaatta actgaagcta agaggatcgt ccacgagatt gacgaaaaag
     3301 ctttcctttc aatcatcaat gtgcatgaag tggaagggca aggctttact tatttgaagc
     3361 cacagacaag attactgaaa aaactataag aaaaaagaca aagataggct atgaggaaag
     3421 attccacatc cagctgacct ttgtcttttt tagtactata ctatttttaa ctaattgtta
     3481 tgttcactta cccggttact tttcttttgt gtctgcaaaa ggatctttcg gttaacttca
     3541 tcagataatt tcaaaaaagc atccctatct ccatcttcta gtgcttgatc gatttgagca
     3601 taaagcattt ttagttttgc ctcttcttct ttttcttgaa aatatcgttc aattaattca
     3661 gtatcttctt cactgactga agaatcccaa gaagaaaatg gattgtcttc taagatagat
     3721 agatattctc ttgtctgcca agcattttcg aatatacatt ccacatataa aggctttttc
     3781 caattcaaac ggatttcatg aaaaatctgg tctgtgtccg taaattcttt tccctgcaaa
     3841 aacaatttca tgggttcccc ttcaacagat agatcactga tctgcagtcc tcgatccgca
     3901 aaattcgccc cttcaacaaa gtggacattg cttaaaatcg cttcatgatt ggcaagataa
     3961 ttcaagatcc aaaaaacttc tcttcggcta aaagatacat gattgaccat ccacgttaaa
     4021 aaattcttct tttcccgtac atcaattagc atttttctca cctctctgtt aggtcatcta
     4081 ataatgacag tgcctccata tctcctggtt caagtgttaa ataataagtc aataattctt
     4141 gcgctctttt cagctgccct tcttctctta agaaaaaggc atactctttc aagaaatctg
     4201 gttcatgtga tagttcatga taagcctgtt catagtgtac agctgcaacg gtaaagtctt
     4261 ctaattcatt ataagcatgg gctaggttcc attctgcata aggattagca gtttcttcca
     4321 tctgattgat cacttgtatc acatcttcat atctttcatc atccaagtaa agattactta
     4381 aagtaagtaa tgtttcatct tgtttgtctc ctacttctaa agctttcagt aaaagttctt
     4441 ctgctcgctg tctatcatgc agacggaaag cattttccga ggccaggtga taaagttcga
     4501 cttggtaagg attttctttg attccttctt ctaacactgt ttgtgcttct tcaatcattt
     4561 cttcttcttg aagagcttgt cctagatata gatataacga ttgataatgc ggattaactt
     4621 ctcgaagttc ttccagatag ttgattgcct gctgattttc tttcaattga agattcgtaa
     4681 aagctagctg gaacagtcga tcatctgtcc tttcttcttt cagtgcttct tctaaaggta
     4741 cgatagctgc ttcgaattct ccttgcatac ttaaggattg tcctaaacgt tcctgcatcg
     4801 agattcctga aatttcattt atcccgcttg ccaacaatgt ttcataaacc actgctgcct
     4861 ctttgaaacg atcaactgaa aaatacaatt cagctagcgc aaactggatc aatggttcat
     4921 caggaagcaa cccagctgct tctttcaatt ttgcttcact cacttcaggg attccgataa
     4981 cttggtacaa atcagctatc gccaataagc tttgcggata gtaatcactt tgcttatcca
     5041 ctttttccag gtattcaaaa gctaagtcga tctcattatc ttcgattgca atttctgcta
     5101 acgggatgtt cagtccgtca ttttctgggt attggttgat cagttgttca aaaatctgtt
     5161 ttgcttcgat caaaaagcca atagccagca attcttcgcc tagttcagct aatatatctg
     5221 ctgggtcttc ttttaatgct ttttctaaaa agatttgtgc ttgtgacaag tcttcatctg
     5281 ataaagcttg caacattttt tcgctatttg tattcatttc aattccctct tatctcactg
     5341 acgatttgtt gaaataccgt cattgttctc aataattctt tttcgtttgg atgttggtat
     5401 ccgtctatta tacctatttt ttctaaaaca agtatttttt tgcttttttc tgccaaaaaa
     5461 agaacttttt ccaaatactc catctttgaa atctgttcca gccagtggat tggatagcca
     5521 agcaagtcca gccacacaaa aaagtttttt agttggaaat caaaaggata tgcttggaga
     5581 ttccacacga aatgaaacat cattcctaaa aattttttca tactagcaga aagtagatgg
     5641 ccattttcgg tcagataaaa agcttgttca aacagtttac cataatttga caggttttct
     5701 gcctctaatt gataaaaata gatcatttct tcgatcatcc cggcaaacgg ccgagtcgtt
     5761 aattgctttt tatttggata gtttttatac agattttgca aaaaacgatg atcacaaatc
     5821 attccgcaac agataaaagt caaaaagtca atctgctttc cgtccacctg ccgctcgctg
     5881 attgtttgat cataaacgat gcgttctggc agattgatgg tttgcagcac tacacgattt
     5941 ggtagctgca aaatcgtccg ctgcggtgtt aatgctcggc ttagagaacg gatcgataca
     6001 gggatcatcc atagtgatga atcaagggtt gtatgttttt gaaaaaaacc tgccagatcc
     6061 atgatccctt catttccaaa tccgatgata aacatggatt gattttcggg atagcgtttg
     6121 acgaaatcaa gcaggttctt taattctgtt aaatgattgc attgagtgtt ggcacaaata
     6181 taccagtcat tgtttgcttg atttgcaaat aacgggttga tcttatctgc aaacaaatca
     6241 taatagcgct gattcgttaa aaataagatt ggctgatcaa tggaaagttc tcttagctga
     6301 ctgactagcg tttctccgta aaacactttc gtcaacgcat cttctacttg ataatcaagt
     6361 tccatacgca tccctcctct tcttattttt attttaccat tagtaagcaa attctagaaa
     6421 agataagagc aaagctcacc tcataacttg gaaataaata aaaattaaat aaaatacatg
     6481 tgttttctat gtatgacata cggattcgga atctctgtct cttatttatt ccttttctgc
     6541 taggaaataa aaagaaaagc acttgtagcc ctagagccac aagcgctttt tcaactgtct
     6601 tatttaacag cgtcttttaa tgctttacct ggtttgaatg caggtacttt gctagctgga
     6661 atttcgattt cttcaccagt ttgtgggtta cgacctttgc gagctgcgcg ttcgcgaact
     6721 tcaaagttac caaaaccgat taattgaact ttttcgccgt tagctaaagc atcttgaata
     6781 gttgaaaata cagcatctac tgctgcagtt gcatcttttt tagttaaatc agtagctgaa
     6841 gcaacttttt cgattaattc tgctttgttt gccatggata gattcacctc ctccacaaga
     6901 gatatgatag aaatatcatt tagttaaaac tttctgagtg gtccagaagt ttaaaaagaa
     6961 atattgatta acttcaatat tctgttatca cgatatcata tgaaccgctt aatagcaagg
     7021 tttttaccaa ttttcacatg ctttttttag tcatagaaac gtaacaaacg aaactgtctt
     7081 tttcttttga aaaaaaaacg tcgatatggt aaaatgccta ttttctccta cgagggataa
     7141 ttcggatgga cgtcccttca aaagtaaatg cttttctgat ttgattttct aagaaacgtt
     7201 cataagaaaa atgcatcaat tcctcttcat tgacaaaaac gacaaatgta ggtggtttga
     7261 cagcgacttg tgtagcataa aaaatcttca ggcgttttcc tttatctgtt ggtgttggat
     7321 tgattgctac agcatccata atgatatcat tcaacacagc cgaagagata cgaagattct
     7381 ggttcatact tacttgttcg atcatttcag gcagacgttc caaccgttgt ttagtcactg
     7441 cagaaacaaa aacgattgga gcgtaatcaa gataccggaa ttcatcacga atctcttgtt
     7501 cgaaatcacg cattgtattg gtttctttct ttacaagatc ccatttattc acgacgataa
     7561 taattccacg accagcttca tgggcgtaac ccgctactcg cttgtcttgt tcccgaatcc
     7621 cttcttcagc attcagtacc atcaaaacga tatcagaacg gtcaatggca cgcattgcac
     7681 gcatcacgct gtatttttca gtattttcgt atacttttcc gcgtttacgc ataccagctg
     7741 tatctatcat taagaatttc tgaccacttt cagaaacgaa gtgggtgtcg attgcgtctc
     7801 gggtcgttcc ttcaatatcc gaaacaatta cacgttcttc tcctaaaatc gcattgatca
     7861 aagaggattt accaacattt gggcgaccaa tcaaactaaa cttaatcgtg tcgtcgtcct
     7921 cttcttcagt ctcttcagag aaatacttca ctgcttcatc taaaatatca ccaatcccta
     7981 gtccatggct tcctgaaaca ggataaggat cgcctagccc taaggaataa aattcataga
     8041 tatcgtttct catttctgga ttatctactt tattgactgc taaaatcact ggtttattac
     8101 ttctgtataa aattttcgca accatctcat ctgcgtctgt cacgccttcg cgtccgctga
     8161 ctacacaaat gattacatct gcttcttcaa tcgcgatttc tgcttgatgc ttgatctgat
     8221 ccataaaagg ttcatcgcca agatcaattc cgcctgtgtc gatcacgctg aattctcgac
     8281 ctaaccattc accttttgca taaatacgat cacgagttac tccaggagta tcttcgacaa
     8341 tcgagatacg ctcaccagcg atgcggttaa aaatcgtaga cttaccgacg ttcgggcggc
     8401 caacgattgc aattgttgga tttgccatat tatttcctcc tcacaagttt ccaatctcac
     8461 ttagtttaac gaatgcattg ccaactttca agcatagatt gtcaattaaa cgaagtattt
     8521 ttattcctta ttgaaatact aatcgctttt caaaagaaaa actgagacaa aagcagctgc
     8581 tttcatctca gtcacagata caaaatcaat tatttttcag aatcatcggt tgctttttct
     8641 tctacttctt ctttcaacgc atcacctaaa atgtctccca tagtaaatcc agtattttct
     8701 tctggtaatt cataagactc aacatttttt ggttcttctt ctgaagctgg tttttcttct
     8761 aaagctttga tactcaatgc aatacgatgt tcttctggat ggatctctaa tactttcact
     8821 ttcacttcgt caccttcatg aagcacttca tgtggtgtcg caatatgttt atgtgaaatt
     8881 tgagagatat ggactagccc ttctactcca gggaacactt caacaaaggc accgaagctt
     8941 gtcaaacgtt taactgttcc gtctaatact gtgccaactg gagcttttgt atcgatttct
     9001 gaccaaggtc caggctgtgt gtctttgatt gacaaggaaa cacgttcttg ttcaggattg
     9061 atagacaaca ctttgacatt tacatcatca ttgacagcca atacatcact tggttttgct
     9121 acatgactat gtgaaatctc tgagacatgg actagaccgt caattccgcc taggtcgaca
     9181 aatgcgccaa aatcagtaag acgtgcaaca cgtccactga gaacatctcc ctcatggata
     9241 gttgaaagaa tttctttttt cttttcttct ctttcgtttt ccactaccgc tttatgcgat
     9301 aagatcaaac gattttctga tggttcgatt tcgatgattt taaagctcaa tgttttgcct
     9361 ttatagtctg aaaagtctgc aacaaaatga tcttccacca ttgatgcagg gacaaaacca
     9421 cgtacaccga cgtcaacgac taaaccgcct ttgacaacat ttgtaacagg tgcttcgatg
     9481 accttgccct cttggaaatc tctttcgata tcttcccaaa cttttttagc atctaaacgg
     9541 cgttttgaaa gtaggtagct gccgttttct ttatctttgc cgatcgtact gataacgact
     9601 aaatctaata catcaccgac ttttactact tcgctgatat cttctaccgg caaagttgat
     9661 aattcttttg ccggaacgac tccttcaact ccagcccctt cgattccgac aatcacttgt
     9721 ttgtcttcaa tgactaaaac ttcgccctta acaacatcgc caacttttac ttcttgtacg
     9781 cttttcattg cgtcttccat tgactggttg ttctcttgat tttgatcaaa ttctgtcata
     9841 acacttgtcc tcctaaccaa cattctgccg taagtacggt actgttttta gtttaattga
     9901 ttattcaaaa aaaataaagc gatttcgttc gttttccttg aaaaaacaat tgtttttcat
     9961 tttttttctt aatttaaaaa agaagataac cttttttcaa aacaacagct tttattgctg
    10021 caacgacttc tggaatcgat ttgccagtcg tatcgatacg aacagcatcc tctgcttgct
    10081 tcagcggaga gacgtccctt gttgaatcat aatggtctct ttcctgtatc tctttcgtca
    10141 gtgtttcaag atcagtcgga attccttttt cttggttctc tttgaaacgt ctttctgcac
    10201 gctcacttac gcttgctact aaaaagattt tcacttctgc attcggtaat acagctgtcc
    10261 cgatgtctct tccgtccatg acaacgccac cgcgttcccc tatttttcgt tggacagcaa
    10321 ctaattcttc tctgactttt ccatgtgcag agacttcaga aacagctttt gtgacatctg
    10381 gttgacggat ttctaaagtc acatcttttg atgctatgaa tacatgctgt ccatcttctt
    10441 gttgcgaaaa agagatagga tactttttga tcagcgaaac tagcccttct tcatctgaaa
    10501 aagatacatt gttcgtaata gctaaataag ttactgcgcg atacatggca ccggtatctg
    10561 tataaatata attcaattct tttgctaata tcttggcaac ggtacttttc ccagatgaag
    10621 caggcccgtc gattgcaata ctgatttgtg tcatgatata actccttttt ctcagaaaaa
    10681 aagccgcaaa gcgcggctat tattatttaa ttcttaattc ttgaccaggg taaagcataa
    10741 agttgtttgg atctaagcca ttcaattcaa acaattgatc agtggtgatc cctgcacgct
    10801 cagccacttg ttttggacct tcgcctgctt gaacagtcgt tgttgaaccg gctgcagctg
    10861 atgaagaagg tgttgtttct gctgcattgc tgcttgaatc ccctgcttga taagttgcct
    10921 cacttgagga agtcggtgtt gtctctgctg caatactgct ttctgacgtt acacctgtat
    10981 cttgagacac agtagattct tccgcgattg tactagattc tactgttgaa gtctcagttg
    11041 actcctcttt tgttgtactt gtagaacttt ctactactga tgtcgaagtt tcagaagaag
    11101 ttgctgctgt tgcattgttg cttgatccat ttctgatcca gaaataagca cctgctggaa
    11161 tagcgataca taagaccagt aaaatcaaaa atagcgaaag aaataatgta tttcctttct
    11221 tttgttgtct ctgagaactt cttgatgaaa cttcatcatc tgtatcgtag attggttgtt
    11281 cccatggttc ttgtgtttcg ttattttctg tagattgatg tctatctttt ctgctcactg
    11341 ttttacctcc taaactctat cgtgtgtatt ataccatagg aaaaagtgtc ttgtcctaaa
    11401 aaatcaatgt tccatgtttt ttttaaataa atttagtaaa atcgtttccc aatgaagttg
    11461 ttcagtctta gaaaaaagct tcctctttgg cagaagttct gcttttgctc catcaatatc
    11521 acaacaattc tgcggctttt cggagagttt ttcgccaaaa taagctaaga tgaattttct
    11581 gcggcagttt tcttcattaa tatagcgaag catctgctgc aggcggaatt ctttttcttt
    11641 ttcctgacgt ttgagccgtt ccacccaatt ttctggtttt tcagactgct ggatcagctc
    11701 catccatttt ttttgcagct catcaaaggg agcttgcttt gtggcatatt ccaaataaag
    11761 ctcaaaactt tgtcgctgtt ctctagaaag ttgattaaag aaataatgga tacgttcatc
    11821 cccactttga taaaaaagaa ttgctgcact ttcttcctga tctctgcctg ctcgaccgat
    11881 ttcttgtaca tagttttcta gactgtctgg caaatcataa tggaccacat aacggatatc
    11941 agatttatcg atccccatac caaatgcatt cgtagcaatg agaaactgca gctgattttt
    12001 tacaaactgc tgttgaagtt gtcttctttg agctgcatca agaccaccgt gatagtatcc
    12061 aactgtaaaa cgttccctga ataaatgata caatcgttcg acttcttttt ttgttgcaca
    12121 ataaatgatt gccgctcctt gtgctctttc catgaagtgt tcaagttctt gttctttttg
    12181 ctgcgttttc cgaacaaaaa gcgagatatt ttgccgattg actgattgtc tgatctcttt
    12241 tggtggtcga tcaaatagta catgacgtat atctcttgct actaaatctg ttgctgtcgc
    12301 cgttagtgcc agtgtgaccg ggtttcctaa aagctttcgg atttttccta actgttgata
    12361 ttctggacgg aaatcaactc cccattgaga cacgcaatga gcctcatcta ctacatataa
    12421 cgcaatattt tgttttttca gatgctctaa aagagaagga ttagtcaaca tttcgggact
    12481 caaaaacaaa aatttatatt gcgatagatg acggaggacg tatcttcttt cagattgcga
    12541 aagtaaacta ttgaaagcag ctactcgttt ctcccctcgt ttttgtaatt gcgtcacttg
    12601 atcttccatt agagaaagca gcggtgacac gatgatgacc agaccttcca ttaaataccc
    12661 cgtcaattga tagcaaaggc tttttcccgt tcctgtaggc aaaatagcga gagtatcttc
    12721 tccatcaagc aacgcctgaa taatttcttc ttggccggga cgaaatgaat caaatccaaa
    12781 atattttttt aattcattta ttaacattct gtatcgcttc ctttctcaat agatagattt
    12841 gatacaaacg aaattctcca taatctaatt gtccatctac attccattcg ctaaagcgcc
    12901 actctcgcac ggaatcctta tttggttcca aacgttcgat tgtttctgca gagagaatcc
    12961 actcgtatgg aaaatcatca aaaaacaagg cccactcaat aagatgatct gttactgttc
    13021 ctctttttaa atgacggatt gccatgatct ggtcaattgt ctgaccattc ataaacatct
    13081 gcttggtgat catcatgctt tgattgaaat tttgctgtaa taatgaatcg atcaaagcgt
    13141 gccaaagcga atctggccgt ttttcgattt gtgctaacaa aaggtggata catctgcttt
    13201 gttgcagata aacctctgac tcatcattgt tttttgccag ctgataagga agcagccctg
    13261 ttcgctcatt tccagataat tggttagcta aaaaatctgc tgcttcttct ggtatctccg
    13321 aaaacagctg caccaaactc tcatacgcag tatcaatcaa aatttcacgt ggcaattggc
    13381 tttcactaag ccattttttt agttggaaag tataaaaagg acttgtctct gctggaagat
    13441 agtcttgaat acccgttgcc agattagaaa ttacttgaac agcaaattta atgagccgcc
    13501 aactagtttc ccctgttctg ccatagcggt caaaacgcaa accagtatgt tctatttgat
    13561 gttcggacaa catatcagcc cctgcaggcg ttagctttgc ttcattttca tttatttcga
    13621 tccaaccttg ctgcgcaatt ttttgcaaaa tttgataaaa cttatcctgc ttcaattctg
    13681 gaaaactgtt atggataaac aaaagttcgt gggtaaatcc ataaataagt actgaagtcg
    13741 ttcgttttcc cacaaggagt tggtaaagag aactaatccg cagcttgtgt cctgatgaaa
    13801 atagcgataa aataaaaaca atcatattat tctccttaag ttaggatggt tagaaatgtc
    13861 actactatgt aaacttgtac ctgaacgctg tattgcatgc ggtctttgtc aaatcgaagc
    13921 gccagatatt ttcgattatg atgaagaagg aatcgtttta tttgcccaag aaccagaggc
    13981 tcatcaacag ttcgtgatgc cggaagaaga aacatatgta aaaaaagcgt atcaaaaatg
    14041 tccagttcga gcgattttat tagaaaataa aatataaata taaacgaaac tttaaaaacg
    14101 tacactaagt tctctaaact tgaaaataag ccgggatctg tttctcttgt tccgtgaaat
    14161 cccgacttat tttgaaagat tcatttgcta tctatagtta attgactagt tttttcacgg
    14221 accctatagg acccatttat attatttcaa ctttttcagc cagttttcca atgtattgac
    14281 gatcacagtt tgctggtctg cgttagaaat ttctgcctct ccatctcctt tttgctcacc
    14341 atagctgcca aaacctccat gatttccgcc attgattgat acatactctg ttctttccgg
    14401 taaatactgc ttagcttctt gataagcttc ctgattcaat actttatctt ttgtcgccgt
    14461 gatcgataaa acaggaacag ctgattctgt caatgttcct tttttgtcgg gataacttgc
    14521 catgaagaat acccctttta aatgtttgtc tgaatgattc ttattgacat aacgacttgc
    14581 cattacgcca cctaatgaat ggccaccgat cacataatcg gaatttggaa aatcttcttt
    14641 gaccttatcc gctttgtttc cgcccatcac tgctagatca agtggcattt tgacaatagc
    14701 gactgaaaag ccgttttttg ctaaatcttg cgcccaaata ctataacttt ctggctctac
    14761 gagagcacca ggataaaaaa taagtaatgg agactttcca tgattctttt tctctccttc
    14821 aaaaaataag taatcttctt tgttttttcc ttcttttacc aacacttgcg cttctgacga
    14881 tggttcgtac gtattttgct gtatatagta ataaccggct ccaccgagta atcctaaaat
    14941 gatgactaag ccaattagta ttcgtctaaa tattttcact ttctcccacc ttttctatct
    15001 gagtgacccc tttttcttaa tcaagtatga ttatacctta aaaaaataag agagaaatgc
    15061 aaactttcca gcatcaaata aaaaaagacg gaatatccat ttgaatatcc gccctttttg
    15121 tatgtttatt tttatttagc acataaacga tacacagctt ctgcataaat ttccattgct
    15181 ttatacatac tacttaattc ccatcgttca ttcgcctggt gcataaagtc aggagtatca
    15241 gggaacatcg caccgaatgc cacacactgg ttcatcgtac gagcaaaagt agcgccgcct
    15301 gatacaaacg gttcagtcat gtctccagta atatcgcgat atgtccccaa tagatttttc
    15361 actaactcac tgtctagtgg cacgtataat gaatcaagga agtcaaattc ttcataagtc
    15421 aacccatatt tttctgctgt tttagttaat ttagctacta aatcatcttt cttgaatgta
    15481 acagggattc tcatatctac accgattttt gtttgttctg gtgtgatttc caaacttgca
    15541 aagttcatcg ttagctcgcc ggattgttca tcttctgttt tgcccacgac agcttcacca
    15601 gttgcatttt cttgaactaa ttttcctaag aaattgattg ggccaaaatc aaatacttct
    15661 gaaagagcaa ttgccagacg agaaatagca ttcactcctt gtgctgcatc tttcgcgtgg
    15721 atacttttac caagaacgac gatgccttca ccttgttctt cgaaatcgaa ttcgtgtttt
    15781 ttcaatgctt cttttacttc agaaagcttt tctccagagt aaggagctgc atcaggaaca
    15841 acattcaacg ctcctccagc ttttacagaa aactcgttcg ttcctggtcc tgtcaagtaa
    15901 gcttgcaata agcctttttc tgcataaatt aaaggaaatt cagcatctgg cgcaaatcct
    15961 tgcgtaattc cttcttcttt ttcgttatat ttttctaaac aacgccataa attttcttca
    16021 tctgtaccaa agataaaacg aatacgtgtc ttgaattcca cgccagcatc aatcaaagct
    16081 ttgacggcat acattgccgc aatagaagga cctttgtcat cttgggatcc gcgaccaact
    16141 aaccagccgt cttttatagt tggatcgaat ggttttgttt cccaattatt ttcgtcccct
    16201 gctggaacaa cgtccatatg acacaagata ccgaataatt catctccaga gccaatctct
    16261 gaataaccgt aatagccttc tggatcttta aatgttctaa atcctagctt ttcactgatt
    16321 tccaatactt tatccaaagc ttcaatcact tttttaccga agggatgtcc ttgtcctgaa
    16381 tctgcttcat ctagtacgga aggaattcgg atcaattcgc tcaatgattc taatgcttgt
    16441 tcatgatgtt gttctgtcac aaattttttc atgatattat cctcctaata tgttaaaaag
    16501 ggtcatgtca aattcctccg acacaacctc ttcttctcaa ataatgacgt tttaagcctg
    16561 ctccatcagc aagcagaatg ttcttaacaa atgatgaaag aaaaaacact cactgaatgg
    16621 agctattgac ttgtccacgt gcttatcgat agccgtttat cggtttcttc attcttatga
    16681 tagaccttgt acctgtattt gtctaggttt agaacattgc tgctacacct aggatcacac
    16741 agatggctac aaataatatc gcaattaatt taccggtaaa tttccaccac actgaaacat
    16801 cgaaacgagc aattgctaca gcacccatta cgatagcaga tgttggtgta attaagttta
    16861 ctaaaccaga tgcagattga taagcagtga ttactaaatc cttaccgacg ccggcaaatt
    16921 cacccatagg tcccatgatt cccattgttg cagcagctaa gccggaagtt gaaggaatca
    16981 agaatgacat agggatatag aaaataaatg taataatgat gaatacactt gaagataatg
    17041 aacttaatcc ttgttcgccc caatgtaaga tggtgtctgt gattaatcca ttgttcataa
    17101 ccacttggat accacgagca acagctacta cgattgctac accgatcaaa tcagaagcac
    17161 cagctaaaaa ttcagagaca aattcagact ctttcatgcc ataagtgatt gctactacga
    17221 tggacattac taagaataac atagtaattt ctgggaagta ccagtcacct aatggaagca
    17281 tgcttttacc tagaaccgta cctagtaccg gaaggtttgt taaccagttt gtcaatgatt
    17341 caaaaatcgt gaaattcgca ttcaaggttg tccaaggaat caaactgatg atcatgatac
    17401 caaaagtaat aaagaacaat acattaacat gtttttgttt tttcgtgatt acttcttgac
    17461 gaccgattgc ttcaatatcg aaatgcttca tatcttcttt acgttggttg tatactaatg
    17521 attttgttgg atctttttcg atctttgatg cataacgata cacatataca atagaaatag
    17581 ctaacaaaat aacgaacatc aagaatcgaa gaccaatacc ttcacccatc gaaatcccaa
    17641 cagcttgtga agctacgcct gttgcaaaag gatttaccgt tgaagctaga cagcctactt
    17701 gagaccctac taatacgatt gctacagcta ctactgtatc aaagcctaca gccatcatga
    17761 ctgggatcag taaagcatag aatggaatcg tttcttctgc cattccgtaa gtagagccgc
    17821 ctaatgcaaa taacagcatc aatactggaa taagcatttt ttcttttcct ttgaatttct
    17881 tcacaactga agcaatcccg gcatctagtg cccctgtttt gttgataata cctaaaaatc
    17941 caccaacaac caaaatgaag aaagagattg ggattgcagc aggtgtttct ttagttagct
    18001 cccccgctgg tacacctagc atcccattga ttggcgccat gaaaatatcc cacagccctt
    18061 gaggattttg ttgtactcgt tcatatgatc cagcgacgat gttgcctgca tcgtctacac
    18121 tatagtgccc agctggtata aaccaagtaa gtacagcgat tactgcaata ataataaata
    18181 agaccgtata cgctgaaggc atttgaaatt ttttcttttt tgtttgttcc atcttcttct
    18241 tcctttcttt tccgttagag acaacaattt tggctct
//
LOCUS       DACDPS010000048        17807 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.048, whole genome shotgun sequence.
ACCESSION   DACDPS010000048 DACDPS010000000
VERSION     DACDPS010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.048"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            1003..1683
                     /locus_tag="IWJ07_001775"
     CDS             1003..1683
                     /locus_tag="IWJ07_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP9333353.1"
                     /translation="MIGIEEKKGVQKDVTIDREENLRGHRFYWIGRRAQDIFFSALAL
                     IALSPVMLITALAIVIDDPGGSPIFVQDRVGRNGKLFKFYKFRSMCVDAEAKLEALLA
                     QNEMDGPVFKIKDDPRITRVGKFIRKTSIDELPQLWNILKGDMSIIGPRPALPREVEQ
                     YGDYERQRLFVQPGLSCYWQIAPHRNELSFEEWVELDVKYINERSFITDWKIIFLTLQ
                     AVLFGYGE"
     gene            1712..3025
                     /locus_tag="IWJ07_001776"
     CDS             1712..3025
                     /locus_tag="IWJ07_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAP9333354.1"
                     /translation="MNIILLSGGSGRRLWPLSNDIRSKQFIKLFKDENGEYESMIQRV
                     YRQITTVDPDAKVTIATSKSQASAIHNQLGDKASICVEPCRRDTFPAILLAAAYLHDE
                     LGVGDDESVAVCPVDPYVDNSYYEGVEALEKLVQQNTANLTLMGIAPTYPSEKYGYII
                     PESSNEVSTVKEFKEKPDKATAEKYLAQNAVWNAGVFAFKLGYLLEKAHAMVDFEDYR
                     DLYSKYETLTKISFDYAVVEKETSIQVMRYNGDWKDVGTWNMMAEVMADNIKGKAVMD
                     DTCVNTQIVNELNIPILAMGCKDMVIAASGDGILISDKERSGYMKPYVEKISTEVMFA
                     EKSWGTYTVMDVQEGSMTVKVSMQAGQKLSYHYHNSRDEVWTVVAGQGKAIVDGMKQI
                     LRAGDVVTIAAGCKHTIEAVTNLDIIEVQIGETISVADKYKSSLN"
     gene            3519..3830
                     /locus_tag="IWJ07_001777"
     CDS             3519..3830
                     /locus_tag="IWJ07_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HAP9333355.1"
                     /translation="MNMEATLKLEKRNKLIHFYPGGSREILCRCKYCEPERIQVKTGF
                     SFSVKEESFQVLLCISGDGQVESANGVMKLIRFVKKILYVFANEYWKMLSVERCGLTE
                     N"
     gene            3831..4598
                     /locus_tag="IWJ07_001778"
     CDS             3831..4598
                     /locus_tag="IWJ07_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HAP9333356.1"
                     /translation="MGIEGGYLMSRMRFMNTEIDNLTMGEVLVEVEKMIQDRRCGYVV
                     TPNVDHIVQLETGKELQDVYKNASLILTDGKPLIWISKFYKTPIKEKISGSDIFSLLC
                     KMAAEKGYTMYFLGAAEGVAAKAADNLMKRFPGLNVVGTYSPPYGFEKDEDEMFKIDS
                     QIKRANPDMLIVGLGCPKQEKFMFYNCDRLGVPVSFGLGASLDFEAGNVKRAPKWMSD
                     YGFEWLFRITQDPKRMAKRYLVDDLKIFKLALKYRKK"
     gene            4625..5626
                     /locus_tag="IWJ07_001779"
     CDS             4625..5626
                     /locus_tag="IWJ07_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HAP9333357.1"
                     /translation="MIITKTPFRMSFFGGGTDMEDYFRENGGAVLSTTFDKYCYVNVR
                     HLPRFFDYSTELSYSRTERVTNIEDIEHPAIRNAMKMLDMHEIRLTYEADLPARSGLG
                     TSSSFAVGMLNAFYALKGKYADKKKLVDKAIYLERVLCNEAGGWQDQIAAAYGGFNRI
                     NFNSDGYEVLPIIITPERKKQLNNNLMMFFTGFTRFSSDVQKANNVSGTEDKRVRLKK
                     MYELVDEAEAVLTDKNRDLDDFGRLLDLTWRLKKATGGAISTGSIDELYEKGMAAGAL
                     GGKLLGAGGGGFLVFYVQPEKQNAVRSAMSNLMYIPFEFENGGTQVIHYTPEMYIPK"
     gene            5649..6935
                     /locus_tag="IWJ07_001780"
     CDS             5649..6935
                     /locus_tag="IWJ07_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HAP9333358.1"
                     /translation="MKVVIMAGGKGTRISSVASDIPKPMIKIENKPVLEHEIECLRDQ
                     GFTDIILTVSHLGDIIMNYFGDGSGISPVTGQPFDVKIEYFLEKEPLGNAGALFRLKD
                     NLTEDFLLLNADAMFDVDFNRFVAYHREKGGLVTLFTHPNSHPYDSGLIIADKDGSVD
                     RWLVKEDSRPTYYRNRVNAGLHVISPAVLDVEINTPKIDLDRQLLKPLAGTGKMFCYD
                     SPEYVKDMGTPDRYYAVCEDFKAGRVKGKNLQNKQKAVFLDRDGTINQYVGFLRHIDE
                     FELIKGTEDAVKKINAAGYLAIVITNQPVIARGEVSFEELEEIHNKMETLLGYKGAYL
                     DAVYYCPHHPHKGYEEERPELKFDCECRKPKPGMLIQAATDFNIDLSQSWMIGDGEND
                     IKAGQAAGCKTALVGESEYGQDMTIATLSEFADKIC"
     gene            6969..7622
                     /locus_tag="IWJ07_001781"
     CDS             6969..7622
                     /locus_tag="IWJ07_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9333359.1"
                     /translation="MIDNRLQKHIDLLIKRYPTLEVIKQDIVDGYLIMEECYLNDGKL
                     LIAGNGGSAADSEHIAGELMKSFKTPRPVSREFADKLIAVDPVKGSELAKNLEYGLMA
                     IPLVAHEALSTAYINDVDGLGVFAQQLFGFGRPGDVFLGISTSGNSKNVINATVVARA
                     LGMKVIGLTGAKGGELARVADVCVKAPETETYMIQEYHLPIYHCWCLMLEDKFFGNR"
     gene            7655..8860
                     /locus_tag="IWJ07_001782"
     CDS             7655..8860
                     /locus_tag="IWJ07_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9333360.1"
                     /translation="MNVLFITTQFPYPLDNGGKIGAYNGISVLSRDYDVTVLSFTEEP
                     QYVEEGVSFFREKFNSVKFENPVIHDVHIRNNPMKLVEALAIGYCLRQPYIVSKFYDS
                     KMLQLIDEKFSNNAYWDIIFIDYLNMQSYGQYIREKYKTQFGKYILKDHNIEYDLVLQ
                     EAESSRGLKKKLLQLEYKRTRKYEEAAVNRADYCFSVCDANTEVLKKINDNSYTMLPT
                     YEMLTGVKKNIEKNTILYLGNLSWKSNMQGLVWFLESIYPNIQQRIPDVKITIVGSGP
                     VGTDFSKYSGVDYRGYVKDISHIYDDQAVLIVPLLEGSGIRIKILEAFNNEIAVVSTL
                     VGCDTIGAKDGSELFIADNEKDFATAVVRLLEDMNLNEKVRKNAKLFLKEKYSLTARQ
                     DEFSAIINS"
     gene            8990..10315
                     /locus_tag="IWJ07_001783"
     CDS             8990..10315
                     /locus_tag="IWJ07_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333361.1"
                     /translation="MIVAAMSYHLTGNSNVASLIAVVGVVYAIVKACNNEYLAIWVMF
                     VLGSDIWGIVPYINIGSGVLHWSDFNVIVAIFLCLRFIKVKARSRYGIVYSVMLIFIL
                     IACVQSHSIYGQDVHTTLLTARELLVVISFWPIVKIVYSERITKNEFIDLVCKFTRWT
                     MCIYWFEWILVNMGFNITFLQTGARWGTRIYIDPIFIILYFFINLYAVIYKREGYNST
                     NKSIWNCIFSLLTFVIISQGRSGILYVVITSVIMLLISLKPSKIIRFGILGIALIACL
                     VSVTQIREVVISSFVESQSSDTGNIAYRIAEQDYFENKLEGHELFGVGIPNNHDARAV
                     EYSGKTIDFESKKYNPYYLEDLGAFEIRYRFGIIAYALYIVALAISIIDAGFRRDKSV
                     AAFAGMAFMVFMALNFNLLAYITARPFGFMISLILIEISAFNDEFIYIE"
     gene            10332..11462
                     /locus_tag="IWJ07_001784"
     CDS             10332..11462
                     /locus_tag="IWJ07_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333362.1"
                     /translation="MNLDRLKKIANMISPIIKVINAPLAYFSGKSDYMEKGILWPTVT
                     TYSNNSRKVIQYTTLPKNVDNMAQMQWAGESFEDICIVLQGPVLEDGDFTLNTVKMYN
                     KYYPKVKLVVSTWIGLSEETECKLREAGADVVLCKKPEKTGAYNINLQLTSAYEGVKM
                     AQKLGCSYALKTRTDQRLYANDVLKYFQTLLNIFPSSDCKVVNQRLIFTTYGKSFRYL
                     PFNLCDFLVYGETSELLKLYGIQRDNRASDFGKIIYTEEKEFNKKIFTLLEGKQGVSP
                     YDVFPDFDALYKKYMFAEYYIVYNYFLENIASNESYGDLMDAYYSYLKKYAVVADSEK
                     LLLYWPKYVQYNVKFENELDFYAKLDSKKWMDIYANYTPFSQ"
     gene            11488..12573
                     /locus_tag="IWJ07_001785"
     CDS             11488..12573
                     /locus_tag="IWJ07_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HAP9333363.1"
                     /translation="MKIGIVTIVNGASFGNRLQNYATQKVLENNGYTIETINNCFDKE
                     STLSIVIKNAIYTLFKFIPHFKKSKSIWKRMYTFYQWDKQYIDYSHRKIDASKKNSFV
                     SIKNDYDYFLVGSDQIWNPKYAANKGFELLSFVPNEKRVSLSTSLGVPSIPSDLEKLY
                     VTEWNKFKSISVRENQAAKIIEDLTGTTAAVHIDPTLMLERSEWEAFQKKPDNIDENY
                     ILSFFLTNPPKDTVDVLDKVAKDLKIGVLTLDASWEYFQDITPNHFVYLISHARMIYT
                     DSFHCTVFSLLFGKPVVVFDRFDSEKMNSRLSTLLNKVNKAHFWVGKKADEAMIRAIA
                     SEPEYDIDKLMVSERECVKAYLKEYLQ"
     gene            12596..13411
                     /locus_tag="IWJ07_001786"
     CDS             12596..13411
                     /locus_tag="IWJ07_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333364.1"
                     /translation="MEHLSDCFGKMKIVTTSCAREYFKDDLDKSEYSCFVDSRAAMFF
                     AMGQALAKRRNVVVMLADELTSCYTALTECAFQQVDLIVFAFQTVRHEIDLASLKNCG
                     CNLINCSAQDDIDRVVSNTKGLNQTTVIKINMNCESDKDHICEYIDLPDIPVFFHGSL
                     YSPASKKKYEQYGQVSKYLGYLVGGKDAILIISHNEFLNDISVWINYYSFGEIQPKII
                     IFDSPNDYQYKWLCDLECSVAHSTNRFLSEDLALLQNNGISIVLRGDNYDIVS"
     gene            13395..15113
                     /locus_tag="IWJ07_001787"
     CDS             13395..15113
                     /locus_tag="IWJ07_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylase"
                     /protein_id="HAP9333365.1"
                     /translation="MISSVKNVQILVSLLKQHNIKDIVISPGSRDFQLVHSVETDSFF
                     RTYTVVDERSAAFFANGLSMAIDKPVCVCCTSSTASSNYMAAAKDAFANGIQLVLLTA
                     DRDYRRLYQMEDQMIDQVDMYGAYVRSNVDIPIVKDSDDEWYATRKINEAILELNHGV
                     KGPVQINYQLMDLSAPFVETMPEYRKITRYQEDDLLSSVEIFKQKLLSKKRIMVLCGQ
                     QEENNLSALLEEFFIKYNTIIIKDNYSNIHGEHFIKTVLVTERMSVNAFIDYCPDLVI
                     TIGKHVWSFVKYTLRDKKLKFEHWRISEDGKIVDPFNKLTSVFQCSAEFFMETVTAGV
                     NSNNNLEYYNLWKTRLSKVCYPELVYSNFYAISELVKKIPDSALVHSSILNAARLYDF
                     VSDDKALLSFANLGADGIDGCLPTFLGQAEADKTRPAYLISGDLSFFYAMNSLCGDIP
                     DNAHIFLINNHSGSEFHTNFGKFYNGSLSVDKHIAAGHISNAKDWALENNIKYITADS
                     KESLKSGLDVFINTFDSPVLFEVFTDPDTDTSVLESFYSINKVITPKMYFRKALDIIK
                     KHLHIK"
     gene            15139..15975
                     /locus_tag="IWJ07_001788"
     CDS             15139..15975
                     /locus_tag="IWJ07_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9333366.1"
                     /translation="MAIKSYIKRLLLYIRHGDPKINLDVSYLSEGAMLAGKTVIITGG
                     AKGLGFAIAKSFIAQGASVIITGRDMDSLNNAKCQLGDMCSIRAFDITDAKMYRTFFE
                     DAYETFGRIDILLNNAGISLHEDGFMSVTEKTWDKQMDTNLKGVYFFTQEFIKFVKEK
                     NQMQANIIMMSSERGLCGDSIPYGLSKAAINSLVVGLSKKYISEGIRVNGIAPGVTAT
                     AMTGISEKGNLYNPATIGYRYFLPEEVAQVALFLASSLSNCISGEIIACDQGQYLSRN
                     WG"
     gene            15980..17413
                     /locus_tag="IWJ07_001789"
     CDS             15980..17413
                     /locus_tag="IWJ07_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP9333367.1"
                     /translation="MGILLKKYNSLSEPAKASIWFTLCNVLQKGILFLTTPIFTRMLS
                     TAEYGKYTIFISWYSIIAIFTTLNLSAGVYNKGLIKYENKYALTSSFLGLSSFISLCA
                     LMLYLPFRTVINGLLDLSTEFVILILIESIFEPAFLLWSVKERFDYKYQKLVLITVLM
                     AVLNPGLGIILILSFGEYAIWRVRAYVVVNVVVGMFIGIGILAKGKKLFDKEYWTYAL
                     RFNLPLIPHYLSMTLLNQVDRLMINSMVGASEAAIYSIAYTISSMMNIITTAINGSFI
                     PFTYKAIKDGVYEKIKSVANQLLVLIFVLCSFTMLFGPELIWVVGGEKYMDAVWIIPP
                     VAASVFFMFLYPLFGNIEFYYEKTNYVMFASCVGSIAKIISNYVSIPRFGYISAGYTT
                     LICYMLFSFAHYMFYKKILRENGIKEDMYNIKNIIFLSVLMIVLTIGMTILYFSTILR
                     YSIIIGLVIVAFLKRTQIMKILKNIKN"
     gene            17430..>17807
                     /locus_tag="IWJ07_001790"
     CDS             17430..>17807
                     /locus_tag="IWJ07_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HAP9333368.1"
                     /translation="MERRHGEMRLLIPSAKIVPEELHHLGKLPAIIYPINQKIVFDYL
                     YDQYKDVCSAIDIACYEKMDKVARRLDKYIKSKTVNIIQLKELGDLGRTIYDSLIGCD
                     EPVIINFADTIINDNIYSLECDSF"
BASE COUNT         5818 a         2457 c         3871 g         5661 t
ORIGIN      
        1 tggtatatcc gatataggaa ctgacgagct atttagtatg atacaaaagt atattgatag
       61 ccattaataa attttccaaa aaataatgac agcacaccaa tttttataat ttagcttgtt
      121 gaatcggtat gttcatggga cggttcaaaa aaagaatcga aaaaataaac ggtaaaattt
      181 tagtgaatcc tatgtaataa aatttggata gatggaaaaa cagcacataa ggaggctgag
      241 tttgttgtca tttctgctcc tacaaactat ggtagtgaat gtgttattga attagtgatg
      301 tctgttactt cagagacatt ggggataatt aacttcacta tacctgtagg gtatacaaaa
      361 aatggaagaa aaaatattat caatagattt tatttagttc agaattttta cgtggaagta
      421 aattattgat gataatcttt atccaattag aattattatc gtaggatgtg atgatgatag
      481 ccgtgaagtt gttcaagtat ttaccaatgt ctacaacgca gaaaattagt ttgggttttt
      541 cggattggaa aatttcaatg tataaaatca taaatttgct gtaaatagac agtttggggt
      601 agtaatagcc caataaattg ttcgataaac tcatctttgg ggtgagaata gctcaatcat
      661 tgaatttcaa acctgccttt ggggtgggat tagaggtcaa tatgtaccta cataatactt
      721 tttggaaaat gatggtgttg agaatgtaag tacatttcaa tgtgaaattc taatatagcc
      781 ccaaacgagg tcaataaaaa tgacctaaac tcgtatttct cccctaaacg aggaggagat
      841 agatatgaaa attgaatgtg ccggcgttgg agttggctgc ttttagtagg aatgaacatg
      901 gttcgatgag aactgtttca gatagagtgg tcgatgatca caggccttta agtcgtttac
      961 agatgatgag acttgtaaaa atagagggac gaatggaaaa agatgattgg tatagaagaa
     1021 aagaaaggtg ttcagaaaga cgtaacaatt gatagggaag agaatcttag gggacataga
     1081 ttctattgga ttggacgcag agcacaagat atcttcttct cggctctagc tttgattgca
     1141 ttgagccctg tgatgctaat tacagcgctc gccattgtga ttgacgatcc cggtggcagc
     1201 ccgatttttg tgcaggatag agttggaaga aatggaaagc tgttcaagtt ttataaattc
     1261 cgatctatgt gtgtagatgc tgaagcaaaa ttagaagcct tgttggcaca aaacgagatg
     1321 gatggtccgg tcttcaagat taaagatgac ccaagaatca cgagagttgg taaatttatt
     1381 cgaaagacaa gtatcgatga gttacctcag ctctggaaca ttcttaaagg tgatatgagt
     1441 attattggac ccagacctgc tttaccgaga gaggtggaac aatacggtga ctacgaaagg
     1501 cagcggttgt tcgtacagcc tggtcttagt tgctattggc agattgctcc acatcgtaat
     1561 gaactgtcct ttgaagaatg ggtggaactt gatgtgaagt acatcaatga aagaagcttt
     1621 atcacggact ggaagattat cttcttgact ttacaggctg tattatttgg atatggtgaa
     1681 tgattatata tagtattagg agttgaaata tgtgaatatt attttattat cgggtggtag
     1741 cggtaggcgg ttatggccgc tgtccaacga tattcgtagc aaacaattca tcaaactgtt
     1801 taaggatgaa aatggcgaat atgaatctat gattcaaaga gtttatcgcc agattacaac
     1861 tgttgaccca gatgcaaagg tcactattgc aacaagtaag tctcaggcta gtgcaatcca
     1921 taatcagttg ggagataagg cttcgatttg tgtagaacct tgtcgtagag atactttccc
     1981 tgcaattttg ttagctgcag catatcttca cgatgaactt ggcgtaggtg atgatgaaag
     2041 tgttgcggtt tgtcctgttg atccttatgt agataattcc tattacgaag gcgttgaagc
     2101 ccttgagaag ttggtgcagc agaatacggc taatttgact ttgatgggta ttgcacctac
     2161 atatcctagt gaaaagtacg gttatatcat ccctgaaagt tctaacgaag taagtactgt
     2221 taaggagttt aaggaaaaac ctgacaaagc aacggcagag aaatatttag ctcagaatgc
     2281 tgtttggaat gccggtgtat ttgcgttcaa acttggttac ctgttggaaa aagctcacgc
     2341 aatggtggat tttgaagatt atcgtgattt gtattccaag tatgagactt taaccaagat
     2401 cagctttgac tatgctgtgg tcgaaaaaga aacgagcatt caagtgatgc gttataacgg
     2461 ggattggaaa gatgtcggaa cttggaacat gatggctgaa gttatggctg ataatattaa
     2521 ggggaaagct gtaatggatg atacctgtgt taatactcag attgtgaatg agttgaatat
     2581 tcctattctt gctatgggct gcaaagatat ggtaattgca gcaagtggcg atggaattct
     2641 gatttctgat aaagagcgca gtggctatat gaagccgtat gttgagaaaa tcagtacgga
     2701 agtaatgttt gcagaaaaat cctggggtac atataccgtt atggacgttc aggaaggctc
     2761 catgactgtt aaagtttcta tgcaggccgg acagaagttg agctaccact accacaattc
     2821 tcgcgatgaa gtttggactg ttgtagcagg acagggtaaa gcgattgtag atggtatgaa
     2881 gcagattctt cgtgcgggtg atgtggtgac tattgccgca ggatgtaaac atacaatcga
     2941 agccgtgaca aatctggata ttatcgaagt tcagattggt gaaactatta gcgtcgctga
     3001 caagtataag agttctttga actaaaagtt cgcgaggtag ttattatgga gatactaaaa
     3061 tttcaacccg tgggtaaaga ttatctttgg ggtggagaac ggtttaagac ggaatttgga
     3121 aaaaattgac ttaacaccat taagcttata cgtgggaatg cttagttcat ctagacgata
     3181 actacgtacg gaaatatcaa atatgaagga cagaatggta aaaccgagtt gtggtatgta
     3241 ttggaagcta caccgggggg tgatttatgc ttttgagtac gatgtaacag aagaaatgtt
     3301 acgaaatgct gtgatggaca gatcacttgg gaaacattta tataaagaag aagtacacaa
     3361 gggagatctt ttttatgtca gttcatggta ttggcgctgg agtattagtg gctgaaatac
     3421 aggaaagctc aaatgttacc tatcgagttt acgattatga tcgagtagat aagaatgata
     3481 ataagtgaga attgtacttt gaaaaagcga ttcaggtaat gaatatggaa gccactttga
     3541 aattggagaa aagaaataag ctcattcatt tttatcctgg tggctctcgt gaaatactgt
     3601 gtagatgtaa atactgtgag cccgaaagaa tccaagtaaa gacggggttt tcgttctcgg
     3661 taaaagagga atcttttcaa gttcttttat gtatatcggg ggacggacaa gttgagtcgg
     3721 caaatggtgt tatgaaactc attagatttg taaaaaagat attgtatgtt tttgccaacg
     3781 aatattggaa gatgttaagt gttgagcgat gcggacttac tgaaaattag atggggatag
     3841 agggaggata tctaatgtca cgaatgagat ttatgaacac agaaattgac aatcttacaa
     3901 tgggtgaagt attggtagag gttgaaaaaa tgattcaaga taggagatgc ggatatgttg
     3961 taactccgaa tgtggatcac attgttcagt tggaaacagg aaaagagttg caagatgttt
     4021 ataaaaacgc atctttaatt ttgacggatg gtaaaccatt gatttggatt tcaaagtttt
     4081 ataagacacc aataaaagaa aaaatatcgg gatccgatat attttcttta ctgtgcaaga
     4141 tggctgcaga aaagggatac acaatgtatt ttttaggtgc ggcagagggc gttgcggcta
     4201 aagcggcaga taacctgatg aaaagatttc cgggattaaa tgttgtagga acatactctc
     4261 ctccttatgg gttcgaaaaa gacgaagatg aaatgtttaa aattgattcc caaattaaac
     4321 gagcaaatcc agatatgctt attgtgggtt tgggttgccc taaacaggaa aaatttatgt
     4381 tttataactg tgatagattg ggagtacctg tgtcttttgg tctaggagct agtttggact
     4441 ttgaggcagg gaatgttaaa agagcaccaa aatggatgtc agattatgga tttgagtggc
     4501 tgtttagaat tactcaggac cctaaaagaa tggcaaaacg atatttggtt gatgatttaa
     4561 aaatctttaa attagcacta aaatatcgga aaaaataaac tatattcaat gaggacaatg
     4621 aagtatgatt attacaaaaa caccttttcg catgtctttt tttggcggcg gaacggatat
     4681 ggaagattat tttcgagaaa atggcggtgc agtgctttcc actacttttg ataagtactg
     4741 ttatgtgaat gtgcgtcatt tgcctagatt ttttgattac agcacagagt tatcatattc
     4801 tcgtactgag agagtaacaa atattgaaga tatcgagcat cctgcaattc gtaatgcgat
     4861 gaaaatgcta gatatgcatg aaattcgcct tacatatgaa gcagatcttc ctgcacgttc
     4921 tggtctgggt acatctagtt cttttgccgt aggtatgttg aatgctttct atgctttgaa
     4981 gggcaagtac gctgacaaga agaaactcgt tgataaagca atttatcttg aaagagtcct
     5041 ttgtaatgaa gcaggcggat ggcaagatca gattgcagca gcttatggtg gctttaaccg
     5101 aatcaatttc aactcagatg gctatgaagt attgcctatt attattacgc ctgagcgtaa
     5161 aaagcagctt aacaacaact tgatgatgtt ctttactgga ttcaccagat tttcctctga
     5221 tgttcagaag gcaaataatg tcagcggcac agaggacaaa agagtaagac tgaaaaaaat
     5281 gtatgagctt gtggatgaag cagaagcggt tctgactgat aagaacagag atctagatga
     5341 tttcggcaga ctgcttgatc tcacttggag attgaaaaag gctaccggtg gtgcgattag
     5401 cactggaagt atcgatgaac tttatgaaaa gggcatggct gctggtgcat tgggcggtaa
     5461 acttctcgga gcaggtggcg gcggtttctt ggttttttat gttcagcctg aaaaacaaaa
     5521 tgctgtgcgt tcggcaatga gtaatttgat gtatattccg tttgaatttg aaaacggtgg
     5581 aacacaggtt attcattaca caccggaaat gtatattcca aaataaagaa atcagcgagg
     5641 ataagaatat gaaagttgtt attatggcag gcggtaaggg aactcgcatc tcatcggttg
     5701 caagcgatat tccaaagccc atgattaaaa ttgaaaacaa accggtattg gagcacgaaa
     5761 tcgaatgtct gagagaccag ggattcacag atattattct taccgttagc catcttggcg
     5821 atatcattat gaactatttt ggcgatggtt ctggaatttc accagttact ggtcagccct
     5881 ttgacgttaa aattgaatac tttttggaga aagagccatt aggaaatgcc ggtgcattgt
     5941 ttcgtttaaa ggataatttg accgaagact tcctcctgct gaatgcagat gcgatgtttg
     6001 acgttgattt caaccgattt gtagcatatc accgtgaaaa gggtggcttg gtgacattgt
     6061 ttactcaccc taacagtcat ccatatgaca gcggtctaat cattgcagat aaggatggct
     6121 ctgtagacag atggcttgta aaggaagatt cccgtccaac ttattaccgt aacagagtaa
     6181 atgcgggact ccatgtgatt agtcctgctg ttcttgatgt cgagataaac acacctaaga
     6241 ttgacttgga ccgtcagtta ttaaagccgt tggcaggaac cggaaaaatg ttctgctatg
     6301 atagtccaga atacgtaaaa gacatgggta cccctgatcg ttattatgct gtctgtgaag
     6361 attttaaggc tggaagagtg aagggcaaaa atcttcagaa taagcagaaa gctgttttcc
     6421 ttgatcgtga tggaacgatt aatcaatatg ttggctttct gcgtcatatt gatgagttcg
     6481 aactaatcaa gggtactgaa gatgcagtga aaaagatcaa tgccgctgga tatctggcta
     6541 ttgtaataac gaatcagcca gtaattgcaa gaggtgaagt ttcttttgaa gaattggaag
     6601 agatccataa caaaatggag acacttcttg gatataaggg cgcttatttg gatgcagttt
     6661 attattgccc gcatcatcca cataagggat atgaggaaga gcgcccagag ctaaaattcg
     6721 attgtgaatg cagaaagcca aagcccggaa tgttaattca ggcagctacg gacttcaaca
     6781 tagatttgag tcagtcttgg atgattggcg atggcgagaa tgatattaaa gcgggccaag
     6841 ccgccggctg taagactgca ttggttggcg agagtgagta tggacaggat atgaccatag
     6901 caaccctgag tgaatttgca gataagatct gctaagggat gttttatcac gaaaggagag
     6961 tataaacaat gatagacaat agattacaga aacacattga tttgctgatt aaacgctatc
     7021 ccactcttga agtaatcaag caggatattg ttgacggata cctgattatg gaggaatgct
     7081 atttaaatga tggcaagctg ctgatagcag gaaatggcgg atctgctgct gattccgagc
     7141 atattgcggg tgagctgatg aaaagcttta agacgcctcg ccctgttagt agggaatttg
     7201 cagataaatt gattgcggtt gatcctgtaa agggttctga attagctaag aatcttgaat
     7261 atggtttgat ggcgattcct ctcgttgctc atgaagcgct ttccactgcg tatattaatg
     7321 atgttgatgg cctaggtgtt ttcgcacagc agttgtttgg atttggaaga cctggcgatg
     7381 tattccttgg tatttcgaca tccggcaata gcaaaaatgt gataaatgca actgttgttg
     7441 ctagagctct tggtatgaaa gtaattggct taactggagc caaaggtgga gaacttgcaa
     7501 gggtagcaga tgtttgtgtt aaagctcctg aaacagaaac atatatgatt caggagtacc
     7561 atttgccaat ttaccactgt tggtgtctaa tgttggaaga taaatttttt gggaatagat
     7621 aaaatatcta agcccatatt gaaaggataa aattatgaat gtactattta taacaactca
     7681 atttccatat cctctagata atggcggaaa aattggtgcg tacaacggta tcagtgtttt
     7741 atctagagac tacgatgtga cggtattgtc tttcaccgaa gaaccgcagt atgttgaaga
     7801 aggtgtcagt ttttttcgag agaaatttaa tagtgtaaaa tttgaaaatc ctgtgataca
     7861 tgatgtacac ataagaaata atccaatgaa attggtagaa gctttagcaa tagggtattg
     7921 tctacgccaa ccttatatag tttcaaagtt ttatgattca aaaatgctgc agttaataga
     7981 tgaaaaattt tctaacaatg catattggga tatcattttt attgactatc tgaatatgca
     8041 aagttatggt caatacatac gggagaagta taaaacacaa tttggtaagt atatcttaaa
     8101 agaccacaac atagaatatg atttagtcct acaggaagct gaatcaagtc gaggtttaaa
     8161 aaagaagttg cttcagttag agtataagcg tactcgaaag tatgaagagg ccgcagtaaa
     8221 tagggccgat tattgcttta gtgtgtgtga tgcaaatacg gaagtactga aaaagattaa
     8281 tgacaacagt tatacgatgc ttccaactta tgaaatgctt acaggggtga aaaaaaatat
     8341 agagaagaac acgatcctat atttgggtaa cctttcttgg aaatccaata tgcaaggatt
     8401 agtttggttt ttagagtcaa tttatccaaa tattcaacaa agaattccgg atgtaaaaat
     8461 cacaattgtc ggtagcgggc cagttggaac agattttagt aaatattcag gcgtggatta
     8521 tcgtgggtat gtaaaagata tttcacacat ttatgacgat caagcggtgc taattgtacc
     8581 tttgttagaa ggtagtggta taagaataaa aatattagaa gcgttcaata atgaaattgc
     8641 agttgtatct acattagttg ggtgtgatac gattggcgca aaagatggaa gtgagttatt
     8701 tatagcagat aacgaaaaag attttgcaac ggctgtagtt agactgcttg aagacatgaa
     8761 tttgaatgaa aaagtaagaa agaatgcaaa attattctta aaagaaaaat actccttaac
     8821 ggctagacaa gatgagttta gtgcgataat taacagctaa tatatatata agcttatctg
     8881 atgatgattt ataggaggaa atatatggag atggctgaga gaaaggaaaa gaaacttgga
     8941 aataagaata tcatattatt ttagaaaact cctgttattt tatgtttgtg tgattgttgc
     9001 ggctatgagt taccacctta caggcaattc caatgtagcg tcattaattg ctgtagtagg
     9061 agtagtttat gctattgtaa aggcgtgtaa taacgagtat ctggctatat gggttatgtt
     9121 tgtattaggt tctgatatat gggggatagt gccatatatt aacattggta gtggtgtgtt
     9181 gcattggtca gattttaatg ttatagtagc gatatttctc tgcttgagat tcatcaaagt
     9241 taaagctagg agtagatacg ggattgtcta tagtgtaatg cttatattta ttttgattgc
     9301 atgtgtgcaa tcgcattcaa tctatggaca agatgtacat acaacgctct taacagctag
     9361 agaattgcta gttgtaatat ccttttggcc gatagtaaaa atagtatatt cagaaagaat
     9421 aactaaaaat gaattcattg atttagtgtg taaattcaca agatggacaa tgtgtatata
     9481 ttggtttgaa tggattcttg ttaatatggg attcaatata acctttttac agacaggtgc
     9541 acgctggggt actagaatct atatagatcc gatatttatc attttatatt tctttataaa
     9601 tctatatgcg gttatatata aaagagaggg ctataatagt acaaataaaa gtatatggaa
     9661 ttgcattttt agtttactga catttgttat aatctcgcaa ggcagaagtg gtatattata
     9721 tgtagtaata acctcggtta ttatgctttt aatttcgctt aaaccatcca agattattag
     9781 atttggtata ttaggaattg ccttaattgc atgtctagtg tctgtgaccc aaattagaga
     9841 ggtcgttata tcttcgtttg tagagtctca gagcagtgac acaggtaata tcgcatatag
     9901 aattgctgaa caagattatt ttgaaaataa attagaaggt catgaactat ttggagttgg
     9961 tataccaaac aatcatgatg ccagagctgt agaatattca ggcaaaacaa tagattttga
    10021 gtctaaaaaa tataatccct attatcttga ggacttaggc gcttttgaaa ttcgttatag
    10081 atttggtata attgcatatg cgttatatat agttgcttta gctataagta ttatagatgc
    10141 cggatttcga agagacaaat cggtcgccgc atttgcgggt atggcattca tggtgtttat
    10201 ggcgcttaat tttaatttgc tagcgtatat tacagcaaga ccgttcggat ttatgatttc
    10261 tttaattttg atagaaatat cggcattcaa tgatgaattc atatatattg aataggtaag
    10321 ggagagcaca gatgaattta gatcgattaa agaaaatagc aaatatgatt tcaccaatca
    10381 taaaagtgat aaatgcgccg ttagcgtatt ttagtgggaa atcagattat atggagaagg
    10441 gaatattgtg gcctactgta acaacctact ccaataacag tagaaaagtg attcaatata
    10501 caactttacc taaaaacgta gacaacatgg ctcaaatgca atgggctggg gaaagttttg
    10561 aggacatatg tatagttctt caaggtccag tattagaaga cggtgatttt acactaaata
    10621 ccgttaaaat gtataataaa tactatccta aagtaaaact tgtagtgtct acttggatag
    10681 gattatctga agagacagaa tgcaagttgc gtgaagctgg tgcagatgtt gtattatgca
    10741 agaaacctga aaaaacaggt gcgtacaata ttaatctaca acttacttct gcatacgaag
    10801 gcgtaaaaat ggcgcaaaaa ctggggtgta gttatgcgct gaaaacgcga actgatcaaa
    10861 gactatatgc aaatgatgta ctaaagtatt tccaaacatt attgaatatt tttccatctt
    10921 cggactgtaa agtagtgaat cagcgattaa tatttacgac ttatggaaaa tcatttagat
    10981 atcttccgtt taatttatgt gattttttgg tatacggaga aactagtgaa ttattaaaat
    11041 tatatggtat tcaaagagat aaccgagcat cagactttgg gaaaattatt tatactgagg
    11101 aaaaagagtt taataaaaag atatttacac tactagaagg aaaacaaggt gtatctccat
    11161 atgatgtttt tccagatttt gatgcactat ataaaaaata tatgtttgca gaatactata
    11221 ttgtgtataa ttatttcctg gaaaatattg caagtaatga atcttatggt gacttaatgg
    11281 atgcctatta tagttattta aagaaatatg cagttgttgc tgattcagaa aaattgttac
    11341 tatattggcc gaaatatgtt caatataatg ttaagttcga aaatgaactt gatttctatg
    11401 cgaagttgga ttcaaaaaaa tggatggata tttatgctaa ttatacacct tttagtcagt
    11461 gattatatgg aaaggatgag gtgaataatg aaaatcggaa tagtgactat tgttaatggg
    11521 gcaagttttg gtaacagatt acaaaattat gcgactcaaa aagtgttgga aaataacggc
    11581 tatacaattg agacaattaa taattgtttt gacaaagaaa gtaccctatc cattgttatc
    11641 aaaaacgcta tttatacatt atttaagttt ataccgcatt tcaaaaagtc aaaaagtatc
    11701 tggaaaagaa tgtatacatt ttatcaatgg gacaagcagt atatagatta ttctcatcgt
    11761 aaaattgatg catctaaaaa aaacagcttt gtgagtataa agaatgatta tgactatttc
    11821 ttggttggta gcgatcaaat ttggaatcca aaatatgcag ccaataaggg atttgaactt
    11881 ttatcatttg ttccaaatga aaaaagagtt tcattatcaa ccagtttggg agttccatct
    11941 ataccaagtg atttagaaaa attatatgtg acagagtgga ataagtttaa atcaatatcg
    12001 gtacgtgaaa atcaagcggc aaaaataatt gaggacttga caggcacaac tgcggctgtt
    12061 cacattgacc caactttaat gttggagcgt tcggaatggg aagcctttca aaaaaaacca
    12121 gacaatattg atgaaaatta catcttgtcg tttttcttaa ctaatccgcc taaggatact
    12181 gttgatgtat tagataaggt cgctaaagat ttgaaaatag gtgttctcac cttagatgcg
    12241 tcttgggaat attttcaaga tattactccg aatcattttg tttatttgat ttcacatgca
    12301 agaatgattt atacagattc attccactgc acagtttttt cattgctatt tggaaaaccg
    12361 gttgtggtat ttgaccgttt tgatagtgaa aaaatgaatt ctaggttaag tacattgtta
    12421 aataaagtaa ataaagcaca tttttgggta ggaaaaaaag ctgatgaagc aatgattcgt
    12481 gcaatagcat ctgaacctga atatgacata gataagctga tggtatcaga gagggaatgt
    12541 gtaaaagctt atcttaaaga gtatttacag taaaattttg aaatgggatg aaaatatgga
    12601 gcatttaagt gattgttttg gtaaaatgaa gattgttacg acttcatgcg caagagaata
    12661 ttttaaggat gatttggata aatcagagta ttcatgtttt gttgattctc gagcagctat
    12721 gtttttcgca atgggacaag ctttagctaa aagaaggaat gtagttgtaa tgcttgctga
    12781 tgaattgaca tcgtgttata cggcactaac agaatgtgca tttcaacaag ttgatttaat
    12841 tgtgtttgct tttcagacag tcagacatga aattgattta gcatctttga agaattgtgg
    12901 ttgcaatcta attaactgta gcgcccaaga cgatattgat agagttgtgt ccaacactaa
    12961 ggggttaaat cagacaacag taattaaaat caatatgaat tgtgagtcgg ataaagatca
    13021 tatatgtgag tatatagacc tgccagacat acctgttttt ttccacggta gcttatactc
    13081 acctgcaagt aaaaagaaat atgagcagta tggacaggta tcgaagtatt taggctatct
    13141 cgtgggggga aaagatgcaa tactcataat ttctcacaat gagtttttaa acgatatcag
    13201 tgtatggatt aattattatt cttttggaga aattcaaccc aaaattatta tatttgacag
    13261 tccgaatgac tatcaatata aatggctgtg tgacttagaa tgcagtgtag ctcattcaac
    13321 caaccgattt ttgtctgagg atttagcctt attgcaaaat aacggaattt ccattgtgtt
    13381 aagaggtgat aattatgata tcgtcagtta aaaatgttca aattttagta tctttattaa
    13441 aacagcataa tattaaagat atagttatct cgccaggtag tagagatttt caattggtgc
    13501 atagtgtaga aacagacagt ttttttagaa cctatacggt tgtagatgag cgcagtgctg
    13561 ccttttttgc taatggactt tctatggcga ttgataagcc cgtgtgtgta tgttgtactt
    13621 cttcaactgc ctcttcaaat tatatggcgg cagctaaaga tgcttttgct aatggcatcc
    13681 aacttgtact tcttactgct gatagagatt accgccgctt atatcagatg gaagatcaga
    13741 tgattgatca ggtagatatg tatggggctt atgtgcgttc taacgttgat attcctattg
    13801 ttaaggattc tgatgatgaa tggtatgcta ccagaaaaat aaatgaagca atattggagt
    13861 taaatcatgg cgttaagggt cccgtgcaga ttaattatca attaatggat ttaagtgcgc
    13921 cgtttgttga gaccatgcca gagtatcgta aaattacaag atatcaagag gatgatttac
    13981 tgtcatcagt agagattttt aaacagaaac tattatcaaa aaagagaata atggttttat
    14041 gtggacagca ggaagaaaat aatttaagtg ccttattgga agagttcttt attaaatata
    14101 atacaattat aatcaaggat aactattcta atatacacgg tgaacacttt attaagactg
    14161 ttctagtaac agaaagaatg agtgtaaatg catttattga ttattgccca gatttggtaa
    14221 taactattgg taaacatgta tggtcttttg tgaaatatac tcttagagat aaaaagctta
    14281 aatttgagca ttggcggata tctgaggatg gtaagattgt tgatccgttc aacaagctaa
    14341 catctgtctt tcagtgctca gcggaatttt ttatggagac tgtcactgct ggtgtgaata
    14401 gcaataataa tcttgagtat tataatctat ggaagacaag attatcaaaa gtttgctatc
    14461 cagagttagt ctatagcaat ttttatgcaa tttcagaact agtaaaaaaa attccagata
    14521 gtgcattagt tcattctagt attttaaatg cggctagatt gtatgacttt gtatctgatg
    14581 ataaagcatt actcagtttt gctaatttag gggctgatgg tattgatggt tgtttaccaa
    14641 catttttagg tcaggcagaa gcagataaaa ctcgacctgc gtatttaatt tcaggagact
    14701 tgagtttctt ttatgctatg aatagtttat gtggtgacat tccagataat gcacatattt
    14761 tccttattaa caatcattct ggaagtgaat tccatacaaa ttttggtaag ttttacaacg
    14821 gatcattaag tgttgataag catatagctg caggacatat ttcaaacgca aaggattggg
    14881 cattagaaaa taatataaaa tacattactg cggattctaa ggaaagtctc aaatcggggt
    14941 tggatgtatt tattaataca tttgacagtc ctgttttatt tgaggtcttt acagatccgg
    15001 atacggatac gtctgttctg gaatcttttt atagtataaa taaggtaatc acaccaaaaa
    15061 tgtattttag aaaggcgtta gatattataa aaaagcattt acacattaag tagatgttta
    15121 ttgcgaggtg aaacaaatat ggcaataaaa tcttatatta aacggttatt gctttatatc
    15181 cgtcatggag atcccaaaat aaatttagat gtgagttatt tatctgaagg tgcaatgctt
    15241 gctggtaaga ctgtgataat tacgggtggt gcaaaaggac ttggttttgc aattgcaaaa
    15301 tcatttatag ctcaaggcgc aagcgttatt attacaggaa gagacatgga ttcgttgaat
    15361 aatgctaaat gtcagcttgg agatatgtgt tctatcaggg cgtttgatat aactgatgct
    15421 aaaatgtatc gaacgttttt tgaagatgca tacgagacct ttggacgtat tgatatactg
    15481 cttaataatg ctgggatctc gttacacgaa gatggattta tgagtgttac tgagaaaact
    15541 tgggataagc aaatggatac aaatttgaaa ggtgtttatt tctttacaca ggaatttatt
    15601 aagtttgtta aagaaaagaa tcaaatgcaa gcaaatatta ttatgatgtc ctcagaaaga
    15661 ggcttgtgtg gtgatagtat tccgtatgga ttatcaaagg ctgcaattaa ctctttagtt
    15721 gtaggcttgt ctaagaaata tatttctgag ggaattaggg ttaatggtat tgctcctgga
    15781 gtaactgcaa ccgctatgac tggtatttct gaaaagggta atttgtataa tcctgcaacg
    15841 atcggttata gatattttct cccagaagaa gttgctcaag ttgctttgtt tttggcaagt
    15901 agtctttcaa actgtatcag tggtgaaatc atagcctgtg accaggggca atatttaagt
    15961 cgaaattggg ggtaataact tgggaatatt attaaaaaaa tataatagcc ttagtgagcc
    16021 tgcaaaagca tctatttggt ttacactttg taatgtgttg caaaaaggaa ttctattttt
    16081 gactacacct atttttacaa gaatgctttc aactgcagaa tatggtaagt atacgatttt
    16141 tatatcgtgg tattctatta tagcaatttt cacaacgttg aatttatctg caggtgtcta
    16201 taataaagga ctaattaaat acgagaataa atatgcatta acctcgtcat ttttaggatt
    16261 atcgtcattt atttcccttt gtgcattgat gctatacctt ccatttagaa cggttataaa
    16321 tgggctactg gatttatcga cggagtttgt catattgatt cttattgaat caatatttga
    16381 gccggctttt ttgctttggt cagttaagga acgatttgat tacaagtatc aaaaactagt
    16441 actcatcaca gttcttatgg ctgttttaaa tccaggtctc ggtattatat taatcttatc
    16501 atttggggag tatgctattt ggagagtacg agcttatgtc gtggtaaatg ttgtagtggg
    16561 gatgtttatt ggcataggaa tactggctaa gggtaagaaa ttatttgata aagagtactg
    16621 gacatatgcg ctcagattta atttgccatt aataccacat tatttatcaa tgacattgct
    16681 gaatcaggtt gatcgcctta tgataaatag tatggttgga gctagcgagg ctgcgattta
    16741 tagtatcgct tacactatat ctagtatgat gaatatcata acaacagcta taaacggttc
    16801 ttttattcct tttacataca aagcaattaa agatggcgtg tatgaaaaaa ttaaatccgt
    16861 tgctaatcaa ctattagtgt tgatttttgt cttgtgttcg tttactatgc tgttcgggcc
    16921 agaattgatt tgggttgtcg gaggagaaaa atatatggat gctgtttgga ttattcctcc
    16981 agttgcggca tcagtatttt tcatgttttt atatccgctt tttggcaata ttgaatttta
    17041 ctatgaaaag acaaactacg taatgtttgc atcttgtgtg ggctctatcg caaaaattat
    17101 ttcaaactat gttagtattc cccgttttgg atatatttcg gctggttaca cgacattgat
    17161 ttgctatatg ttgttttcct ttgcccacta catgttttat aaaaaaattc ttagagaaaa
    17221 tggtataaaa gaagatatgt ataatattaa aaatattata tttctttctg tcttaatgat
    17281 tgtgttaact attggtatga caatattata cttttctaca attctaagat atagtattat
    17341 tattggatta gttattgtgg cttttcttaa aaggacgcaa ataatgaaaa tccttaaaaa
    17401 tataaaaaat taacaagcaa atcttgggaa tggaacggag gcacggtgaa atgagattat
    17461 tgattccatc tgctaagatt gtaccggaag aacttcacca tcttggaaaa cttcctgcaa
    17521 ttatctatcc gattaatcaa aaaattgtat ttgattattt atatgatcaa tataaagatg
    17581 tatgttctgc tatcgatatt gcctgttacg aaaagatgga taaggtagca agacgattag
    17641 ataagtatat taaatctaaa acagtaaata ttattcagct taaagagtta ggggatcttg
    17701 gaagaactat atatgattct ctaataggat gtgacgagcc tgttattatt aattttgctg
    17761 ataccattat caatgataat atatattcct tggaatgtga cagcttt
//
LOCUS       DACDPS010000049        17093 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.049, whole genome shotgun sequence.
ACCESSION   DACDPS010000049 DACDPS010000000
VERSION     DACDPS010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17093
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.049"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(18..923)
                     /gene="xerC"
                     /locus_tag="IWJ07_001791"
     CDS             complement(18..923)
                     /gene="xerC"
                     /locus_tag="IWJ07_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAP9333369.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(1121..2497)
                     /gene="trmFO"
                     /locus_tag="IWJ07_001792"
     CDS             complement(1121..2497)
                     /gene="trmFO"
                     /locus_tag="IWJ07_001792"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAP9333370.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(2466..4544)
                     /gene="topA"
                     /locus_tag="IWJ07_001793"
     CDS             complement(2466..4544)
                     /gene="topA"
                     /locus_tag="IWJ07_001793"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAP9333371.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICDFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(4666..5523)
                     /gene="dprA"
                     /locus_tag="IWJ07_001794"
     CDS             complement(4666..5523)
                     /gene="dprA"
                     /locus_tag="IWJ07_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAP9333372.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(5579..6346)
                     /locus_tag="IWJ07_001795"
     CDS             complement(5579..6346)
                     /locus_tag="IWJ07_001795"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAP9333373.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(6339..7199)
                     /gene="ylqF"
                     /locus_tag="IWJ07_001796"
     CDS             complement(6339..7199)
                     /gene="ylqF"
                     /locus_tag="IWJ07_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAP9333374.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            7459..7857
                     /locus_tag="IWJ07_001797"
     CDS             7459..7857
                     /locus_tag="IWJ07_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP9333375.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            8094..8636
                     /locus_tag="IWJ07_001798"
     CDS             8094..8636
                     /locus_tag="IWJ07_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP9333376.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(8719..9270)
                     /gene="lepB"
                     /locus_tag="IWJ07_001799"
     CDS             complement(8719..9270)
                     /gene="lepB"
                     /locus_tag="IWJ07_001799"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP9333377.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(9422..9649)
                     /locus_tag="IWJ07_001800"
     CDS             complement(9422..9649)
                     /locus_tag="IWJ07_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAP9333378.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(9646..10164)
                     /gene="msrA"
                     /locus_tag="IWJ07_001801"
     CDS             complement(9646..10164)
                     /gene="msrA"
                     /locus_tag="IWJ07_001801"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP9333379.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(10184..10897)
                     /locus_tag="IWJ07_001802"
     CDS             complement(10184..10897)
                     /locus_tag="IWJ07_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP9333380.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(10900..11796)
                     /locus_tag="IWJ07_001803"
     CDS             complement(10900..11796)
                     /locus_tag="IWJ07_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP9333381.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(11954..12796)
                     /locus_tag="IWJ07_001804"
     CDS             complement(11954..12796)
                     /locus_tag="IWJ07_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9333382.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            12928..13581
                     /locus_tag="IWJ07_001805"
     CDS             12928..13581
                     /locus_tag="IWJ07_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP9333383.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(13644..14171)
                     /locus_tag="IWJ07_001806"
     CDS             complement(13644..14171)
                     /locus_tag="IWJ07_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9333384.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(14191..15138)
                     /locus_tag="IWJ07_001807"
     CDS             complement(14191..15138)
                     /locus_tag="IWJ07_001807"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP9333385.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(15150..17036)
                     /locus_tag="IWJ07_001808"
     CDS             complement(15150..17036)
                     /locus_tag="IWJ07_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9333386.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
BASE COUNT         4537 a         3542 c         2987 g         6027 t
ORIGIN      
        1 tgttcttttc cttattttta cgctcttgga tggaaagaac gatagttttt ttgtaaactt
       61 tcttttgtta catgggcata gatctgggta gtcgataaat tggcatgtcc tagcaattct
      121 tggactgtcc gcaaatctgc cccattattt aataaatgag tagcaaatgt atggcgcagc
      181 atgtgaggat ggatatcact atccaaactg ctttttttga tcatttgatt caagacatat
      241 tcaattccag taggcgtgat tggttttcca tgatgattca caaaaacgat atcatgctct
      301 tcgtgatatt ttgtcatcaa tacctggcgg ccttccctga aatattcttg cagagcatct
      361 tgagcaaaag aacctagagg agcatatctc tctttgttcc ctttaccatg gatcaacatg
      421 acctctgaat caaaatcaat gtctgatacc gacaaactgc tacactcact taaacggatg
      481 cctgacccgt aaaggacttc cagtaaagcg cggttcctca aatccaaagg actatctcct
      541 gctacgctgt caaaaagtgc ttgcatttca ttttcataaa aaaaacgtgg cagtctcaaa
      601 tttttctttt tgagatgaac gtaagaaaat ggattttctt ttatcacttc ttctttgaga
      661 agatactgat aaaacgaacg taggctggct atcttgcgac tgatagaatt tctgctatag
      721 ctaggacgat gatcagctaa tgcactcaaa tagacacgaa ccgtaaaata ttccgccttc
      781 agcagatcag cgtctccaga ttcctccaaa aaatgtttga aatggagaat gtcttcttca
      841 tatgcttctt tcgttttttc tgaatatcct ctttcgacaa tcaaataacg caaaaatttt
      901 tgttcccacg tggcttctgt cattttctca ccttcctttt gtcccagaga ataatttagc
      961 acagcgttta taaaaagaca aataaattgt cagaatttaa ccaatatttc taagtttgtt
     1021 cacaattggc gcaattcctc acattttgtt tgagaaattc ttaagaaaaa cgccagaaaa
     1081 agagcgagac tatccgtctc accctcttca tggagtttcc ttactcattt gggattgcag
     1141 ttttttctaa ttctgttttt attgcctcat tagcttttaa agctcgttgt gcaattgctt
     1201 catatcttgc ttttttgtct cgaatgcgct ctggcagttc aggaaacaga ccatagttgg
     1261 cattcatcgg ttggaaatgt tttccttccg catgagtaat gtagtaagcc atacttccaa
     1321 tagctgtttc ttgtggaaac tcgactggtt ccatttcttt tgctaagcgt gcggcgttga
     1381 ttccagcaag tagtccgctc gctgcacttt caacatagcc ttctacacca gtcatctgac
     1441 ctgcaaagaa cagatcttcg cgtttcgttg attggtaagt cggtttcagc aattctggcg
     1501 aattcataaa gctgtttcgg tgcatcacac catagcgaac gaattcagca ttttctaacc
     1561 caggaatcat ccggaaaaca cgtttttgct cgccccattt caagtgagtc tgaaagccca
     1621 cgatgttgta taaagaagct gcagcattgt cttgacgcag ctggataaca gcataaggac
     1681 gtttgccagt tttaggatct tccaatccaa ctggtttcat aggtccgaaa agcatcgttt
     1741 tgaatcctcg ctgtgccatt acttcaattg gcatacatcc ttcaaaatat ttttctttct
     1801 caaaagattt aagttctgcc acttcggcat tgattagtgc ctcatggaaa gctttgaatt
     1861 cctcttctgt catcggacag ttcaagtaag ctgcttctcc tttgtcatag cgggacttca
     1921 aatatacttt atccatatcg atcgtagctt tgtctacgat tggtgcagct gcatcataga
     1981 aataaaaacc atctgaacca ttgaatgctt tgatgctttc tgctaatgga tgagaagtca
     2041 aaggtcctgt tgctactacg gtgattcctt ccggtatttc agtaatttct tcgtttatca
     2101 ctgttactaa tggatgctct ttgacttttc gggtaattgt ttcagaaaaa gaatcgcggt
     2161 caacagctaa ggcaccacca gctggcactg ctgtttcatc tgccgaagtg attactaccg
     2221 aattcaatcg gcgcatttct tctttcaata accctactgc attagccaaa ctgtttcctc
     2281 ttaatgaatt ggaacatact agttcagcaa aattacttgt gtgatgcgcc tcagtcgatt
     2341 tgactggacg catttcatat aaacggacag ggactcctgc ttgtgcgatc tgccaagcag
     2401 cttcacttcc ggcaagccct gctccaacaa catttaccat tttcaatgaa agaacctctt
     2461 ttctattatt tttggatgtc ttcttcgtaa tctccattta cacagactac ttgtttaccg
     2521 cctttgactt tcttctcgac cagatatccg tcacattttg gacaatttct gcccactggt
     2581 ttatcccaag aagtgaattc acattctggg aaacgggaac aaccatagaa tagtctgttt
     2641 ttcttagatt ttcgttcaat gacttgtcct tcatgacata ctgggcaagt cacaccgatt
     2701 tcttttacga ttggtttcgt attccgacaa tcaggaaaat tactgcaagc ataaaacttc
     2761 ccatatttcc ctaattttat caccatcgga tgtccacaga catcacaatc gaatcctgct
     2821 ggttcatctt tgatttgaat tttttcgatt ttttcttccg cattcgtcag ttctttttca
     2881 aacggtttat agaaacgatc aaccactttg acccacgctt ccgtgccttc ttcaatttta
     2941 tccaaatctc cttccatttc tgctgtgaaa tggatatcga cgatttgagg gaagaaatca
     3001 cagattaatg aattgacaat ttctcctagt tcagtcggct caaatctttt ttgtgtcaat
     3061 tttacatagt agcgtctttg gatcgtatca agtgttggtg cataagtgga aggtcgccct
     3121 accccgtttt cttctagtgc acgaatcaac gtagcctcac taaaacgtgc tggtggttga
     3181 gtaaaatgtt gttttggttc aatatcaaca gattggacta catcgccttc ttcaagttct
     3241 ggaagaatat tttctttctc ttctttgccg tcatctctac cttccacata cacttgcatg
     3301 aatcctttga atttgatttt tgaaccgttc gcaatgaaga tgacaccatt ttgttctaat
     3361 gtcactttca ttgtatctaa aatagctggt gtcatctgac tagccacaaa acgtgaccag
     3421 atcaatgtat aaagtttcaa ttgatcttta tctaaatatt gtttgagttc atctggtgta
     3481 cgaagcacac tcgttggacg cacagcttca tgggcatctt gtgcgccttg agcatttttg
     3541 atctttcggt gatcatgtct agaaaattct tttccatacg tatcttcaat aaatgctgcg
     3601 gcctctgctt ttgctgtatc ggcaatccgg gtagaatcag tacgcatgta tgtaatcaag
     3661 ccgactgttc cttgtttacc caaagtgatt ccttcatata attgctgtgc taccatcatg
     3721 gtttttcttg ttcgaaagtt taacttacgg gcagcttctt gttgcaggct acttgtagtg
     3781 aaaggattcg cagggtttct tttgcgctct ttcttttcta cttttttaac ttcgaagtct
     3841 gttccatcaa ttctagcagt gacttctttt acactttgtg catctggtaa tttctttttc
     3901 ttaccgtcga ttccccagaa atttgctttg aatttcttgc gagctttttt aaaattaccg
     3961 tcgatgctcc aatattcttc tggaacaaat tctcggatct ctttttcacg gtcaataata
     4021 atttttaaag cgacagattg gacacgtcct gcacttaacc ctttcttgac cttacgccag
     4081 agaataggac tgatcgaata accaacgatt ctgtctaagg tacgacgagc ttgctgtgca
     4141 tctactaaat ctacatcgat cgttcttggt tccttaaaag ctgctttgac tgcgtctttt
     4201 gtgatttcat tgaagaccac ccgatttttt tctttcagat ccaacccaag taggtacgct
     4261 aaatgccaag caatcgcttc tccttctcta tccgggtcac ttgccaagta aactttttga
     4321 gctttttttg ctgcggcttt caggcttttg atgacatcgc cttttccgcg tatagaaata
     4381 taatggggtt cataattgtt ttcaaaatcg atacccattt tacttttcgg taaatctctt
     4441 atatggccaa cactggccat tactttatag tttcgtccaa gatatttctc aattgtcttg
     4501 gcttttgcag gtgattctac aatcactaaa tatttatacg ccattactga atggactcct
     4561 ttccattgtc gtgttcctac tattaaaaga ggattcatac aaatcgtagc taatcatatc
     4621 cattcagaaa acgtttgtca agaaaagcaa atgaaaagta tggtgttatt ctttaaattc
     4681 tgccaagata tcttcacccg aaaccgtgca aactgcaccg tcttggatca aatgatgaca
     4741 tccggtagaa tgttctgtta aaatatttcc tggaacagaa aaaactgttc ttccaagatc
     4801 caatgcttgc tgtgctgtta tcagagagcc gctttttttc ttggcttcaa agacacatgt
     4861 tcctttagac aaagctgcga taatccgatt tctcattgga aaacaatgct ttttcgggcc
     4921 ggattgagga ggatattcac tcaaaagaag atgatcattt gcaatcacct cttgcaagtc
     4981 acgattttca gcaggataga cttgctctat ccctgttcca atgactgcga ttgtccgtcc
     5041 accatgagag attgctgctt ggtgaccgta tgtatctatc cctttcgcgc agccactgac
     5101 gatcacatat ccagcttcta tcactggttt tagtaaataa taaactgttt ttttggctaa
     5161 agcagaagtg tgtcttccac caacaacgct taacatgtta caagaaagta aagatagatc
     5221 accattataa aacagtacaa ttggcggata ggtcaagtga agcagctgag ggggatattt
     5281 gggatcaaag caagtgataa acgactgttt cgattggatt ttgccccagt tagcgtgaat
     5341 ttcctgccaa cttttgcgaa acagctcccg atgtttggtt attttaccta tcttaatgat
     5401 gtcttcaaat ccgatttctt tataaggata ctgcatcatc cagttagcaa gtgcccattt
     5461 tccttgacta ctgattcctt ttgtataagc aagtttcaat aaattttctt ctatttgata
     5521 catcataaaa ccgcctttcg tctctctttc taataagata cgtaaaagcg gctgtttttt
     5581 attgtaaata tttttttaca ggtgcaaaac ttttacgatg gattggcgta attccttgtc
     5641 gttcaagtcc ttctaagtgt tcctttgttc cataacctgc attatgcgag aaaccatatc
     5701 caggataaag cacgtcatat tctttcatca attggtctcg atatacttta gcaatgatac
     5761 ttgcagcagc aatcgacaca gatcgtgcat cccctttgat tattttttcc tgctgaatag
     5821 gcaaatccaa ggtcatcgca tcgatcaaca aatagcttgg tacaatggac aatttttcta
     5881 ctgcttggat catcgctaat ttacttgctt cataaatatt cacatcatcg attgtctgct
     5941 gatcaacgat cccgatcccg attgccaggg ctttttcttg aacttgttga taaagaagtt
     6001 ctcgcttttt ctctgataat tgtttggagt cattcagccc taatatgatc tcatttttcg
     6061 gcaagataac tgcagctgcc acgacaggtc cagctaatgg tcctcttcct acttcatcaa
     6121 tgccggcaat ccattggtga ccttgtgcgt acccttgctt ttcaaagcaa aacatttctt
     6181 catatttttc cttaattaat gcttcctttt tcaatcgtcg ttcaaaagaa gccagtgctt
     6241 gctgcacgcc tttgcgtgga tcatttctta atgattctag tgtaggatca tctattctct
     6301 gtatgctttt caatcgttct ttgatacttt ggatggattc agtcatcact tttttgctcc
     6361 atttcttcgc aacgatccag tgtgtatcgt ccaagttttc cttgacgaat atcatgcacg
     6421 actaattcgc tgccacgatc atagtcttct ttatacccac gttttgctgt aatatccatc
     6481 agtaattccg gtgccaattt ttcttcttgc tcgattgata aaccataacg atcgctgatt
     6541 cttcctgaat aatatcgggc aaaaaagtct aatccgtaaa tcgctaaatc atctaaatgg
     6601 acaagctggt ctttgatcgc gcctgtcagc gctagttttt tgccgatttc ttcatcttca
     6661 aatttgggcc ataaaatccc tggtgtgtct aacaattcca actcattacc taaacgaagc
     6721 cactgctgac cttttgttac gccaggctta tttcctgtct gtgcaatttt cttgcctact
     6781 agtcgattca tcaacgtaga tttcccaaca tttggaatac ctaagaccat tgcccgaata
     6841 gcgcgaggct tgatcccccg agcagcatct cttgcccgtt tttctttcaa tgcttcttta
     6901 gccgcaggta ctagaccttt aattccttta ctttcttgtg ccgtgatagc taatgcaaga
     6961 tggccttgtt tacgaaaata ttcttgccac agctttgtct gttgtggatc tgccagatcc
     7021 gctttattca ataaaacaag gcgaggtttt tgctgaatga tctgatctag cattggatta
     7081 cgagaagata aaggtaatcg tgcgtctacc aattcaaata cgatgtccac atatttcatt
     7141 ttttcagaaa cttctcgtcg ggcttttgcc atatgtcccg gaaaccattg gatggtcatt
     7201 gttctctcca ttcctttcat accggaatat tccccggctt tacttgtttt tttctttttc
     7261 aatataaatt cactttttta ttataaagaa attccatcta ttagttaagt gaaagtgaac
     7321 cagtcctcat tctatcacat cttcactttt ttgatgatgc aaatttttat tttggtttct
     7381 atatatgaat ctaaattttt gacagcgttt cctaaataag agatgataga cgaacaaaac
     7441 agaaaggaga cgaaacaaat gaatagtatg gtttttgtta attttcctgt tgctgatgtc
     7501 aatcgttccg ttgcttttta tgaaaagctg ggttttaaaa aaaatgaaga attttctact
     7561 gaagaagctg gttcaatggt atgggatgac actttttgga taatgcttct ctctcatgat
     7621 ttttataaaa aatttataaa agataaagaa atagctgata ataaaaaaac aagtggtgct
     7681 ttgatttcgt ttagtgttga aaacgcggat gaagtaaaaa aatttgctca aattgcaaaa
     7741 gaaaatggtg gtactttcca cgaagtagat atgggaatgc ctgaagaaga aatgttttca
     7801 ttagaagtcc aagatctaga cggaaataca ttagaacccg tatggatgaa gatgtaagca
     7861 gataactaat ctgacgtttt ttataacaaa aaaacaaagg cggatgatac gataagtatt
     7921 ctccgcctct atttttttta ttcgtacaat ctctttttta cagtttataa aagcagtctt
     7981 ctcacccttt ttttaattgt tagatgccag tgtatgtaaa tcaccaatgt gtcatgacac
     8041 attggtgatt ttatggtatc cttttattat taactgacaa aggagagaat attttgaaca
     8101 ctttgattgc aaaagacgtt tttagattgc ccgaaatgaa tgcaagattg ttgtcgacct
     8161 ctgctctttt attagccctt gatgtcatct tatacaagat agcgattggt cctgcttatt
     8221 ttcaaatcac attcggattt ttaagtatgg cgcttatggg ttatctatat ggaccgatct
     8281 gggctggttt gcttgaaatg ttgtcgaaca ctttaaattt cacgatattt ggttcaggaa
     8341 cgtttcaagc tgcttttctt atcacggctt tccttggtgg atttattaat ggcttatttt
     8401 tatatcataa aaagataaac ctgccttccg tcatcgctgc ccaattattg attatgatcc
     8461 cagtcagcct ttttatcaat tcttggctga tgtctgtttt gtttggtgca gatttcaaag
     8521 taatttttct aggtagattc atcaggaata tgatccaaat ccctattcaa ataatggttg
     8581 tttattcatt tatcaaaact ttagaggcta gaaaagtgtt taaacgagct gtttgatttt
     8641 acgtactaag gtagtaaact gattttggat taaacaataa agcgtgcagt ctcaacagac
     8701 tgcacgcttt attgtttctc actggatcca ttttatttca tcaagtggat aatagacaaa
     8761 tcttgcttta cctagaatat cagttttatt gatcgtgcca aacgaacgac tatcttttga
     8821 aactcttcga ttatctccaa gtacaaaata attatcttcc ggaagcttgg attgtcccgt
     8881 tagctcttgt agtgtaaagt ccgtcgtata ggatgcattt gcatgatctg atttcaggtt
     8941 tttagttaga taaggttcct taactacttt cccattgatc ttcaattgat cattttggta
     9001 gctgacattt tctcctggaa gaccgattac tcgtttgata taaatcgtgc catctgctaa
     9061 ttgaaaaaca acaatatcaa atcgtctgat ctccgaaaat ttttccatca caaccatatc
     9121 accttgcttc aacacgtttt ccattgaatt gccttccact ggtaccggga tgaataaaaa
     9181 tccccgcaac atgaacgcaa ggaatacaga aacaagtaag tatttgaata caagccagaa
     9241 gcgattaata tagcgttgtt tttttgtcaa tctctatcaa tccatccctc tttttttcat
     9301 tctttcttta ttataagaaa aatatcaaaa aaatgcgact agatagctga aataaaaata
     9361 attctttcaa ttaggctatt ggacaaaaaa cttttttgat cataaagtac aaaactatct
     9421 cttatatgtc atgattattt tctaaatatt tttcccatgc ttcgtcaaag atatccatgg
     9481 acggcaaata gtcgacattc agttccagat aattagaaat ttcttcgtag ctagagctgt
     9541 gcttaggaaa ttggatgtct ttaccggctt ctgtagcgaa aacagataaa gcatcatgtg
     9601 tgccaccgcc tcgcagtgtc attaaataat gaaaaaagct tcgattcacc cttcgttcct
     9661 ccaattttct tcgataaagg cagcccggcg tgcatggttt cttttaaaat tttcaggatg
     9721 ctttttataa tactcttgat ggtcttcttc tgctagataa aatggttggg ctggttcgat
     9781 ttttgtaacg attggctgtt cgaaacgtcc actttcagct aactgtttct tacttttttc
     9841 agcaatgatc cgctgttctt ctgtcaaata aaagatcacc gggcgataat tgtcaccgcg
     9901 atcctcgaat tgtccgaaag cgtcagttgg atcagtttgc tgccagtaaa gttctactaa
     9961 ttgttcatat gaaatcaatg ttggatcata gataatctcc actgcttctg tatgtccagt
    10021 cgtttggctt tttacttgtt cataagtcgg atctggtaca tgccctccag tatatcctga
    10081 catcactgta tggataccag gaagcgtgtc gaaaggctgg atcatacacc agaagcagcc
    10141 tcctgcaaat attgctcgtt ccattatgat tccccctcta atactactca tccgatgatt
    10201 ttttattttc ttttgtcttg ctttcagtac tgctggtgct agttttgttg gtatttgagt
    10261 tgtttgaagc atcagaggta tcttttggca aatatagact aaaccgaatg tcatcattta
    10321 ctaagtcgat tttatctgct ttaatataca tcccgttttg cattcggaat tgatccaacc
    10381 taagcatgat agtcagatca tctgtattga cttcgatcca atctggtaat ttatagtttc
    10441 tcttgatcat attcatcaca tctttgattg gcaaactcaa tgttccgata gaaagacttc
    10501 tagcttttag ttggacattt ccgttttcca tcacatacgg atcaaaatat aaatagaaat
    10561 cgataggaaa acctagtact tcgaaagtac cattgagtag tgcttcattt tccaaataaa
    10621 atttatattc aatatcacta tctttttgat actcactaag gaaaaagctg atgatttgat
    10681 tgactttttc tttatttgaa ttgatcgtta agacaggatc tccttgtctt tccgtaagag
    10741 ctggtatttc tgggagttct ggttcccgat tagcaaaaat acggctgcct aaaaatacgg
    10801 tcgaaccaag tactacccca agaaggataa ggaaggcgaa tttccagtaa tttgttttct
    10861 ttgttttgtt tgaagatacg tcagatcttg ttttcatgtt tattgatcat cctccttctc
    10921 taaccattgg ttttgtgtct cgatcagctg atctctcact gcatttgcca tcaattgata
    10981 acctaaatta tttggatgga aatgatcttc ttcatagagc acattatttt tgatgccatt
    11041 tagattttct gtcgtgctag ttgtttgatc tgttccttct tctgtgacac ctacttcctg
    11101 attcaatcct tgataaagca aatcattgat tgggataaag tagcagttcg gcattttttt
    11161 tgtcaccgtt tctgttcctt tgttccaatt gttaacaatt gtctgcatgt cagtgatctc
    11221 aggaaagttc aaataaaatg gattgtagat gcctaaaatg taaataggtg cggttttatt
    11281 gagggatcga aattcttcca atagttctgt gacattttcc tgatagtttt tcagtggctt
    11341 tttaaaggaa ttgaccgtta acccgaataa attattttgg ataactttca tcaaatcatt
    11401 accgccaaca gtcagtgtga taaaatcagc tgttttgata ttgtttcgaa tttcttcttt
    11461 tgtttttact cgctttaaga tctggtcact tcgttcacca tttacaccat agttttctgt
    11521 ttcaaccgcg gtcaaattat aacggtcttt tagatcattt gcgacaaggg gaacgaatcc
    11581 tccttgcttg gtttgatctc ctactccttc agtcagcgaa tctccgatag ctatgtagtg
    11641 aagtttttct tttttatttg gatcttctct cacagtagtt actggtttca ttagtgggcc
    11701 agctttcggt atagaagcag ctaaacctgc atagatgatg atcgcaatta ataatgggaa
    11761 caaaacgagg atcaaatgtc gtttcgttaa tttcattttt tcacctcttg tattcgattg
    11821 gttctaatgt tatcaaaaat tttatcaaaa aggtagtctt tatgaaaaaa gaaaacaatt
    11881 attttcctaa ttttataaag aaaaagagaa taagctgcaa gcctttcagg ctgagggctt
    11941 atcctctttt cgactaatct acataataca tgattgcaaa cgcgtcttta ccagtatgcg
    12001 tagcgataac cggattcgta tgcagtacag ggatatgcat gtccgggaat aattcttgca
    12061 aaccctcttt gaattcttgc gccaattctt ttgcatccgc atgtgaaata ccgatttgtc
    12121 gcacatttgg caattctttc agttcatttt tcaaattatt aaaccattta tcgaacgttt
    12181 tctttcctcg ccccttagtt acagggatta attcagaatg ggcgaaattc atcaccacgc
    12241 gtatgttcaa aacactagac agcaaaccag ttgttcggct gattcttcca ccttttacca
    12301 agttatctag tgtggatatt ccgatgtata attttgtatt gtcttgtact tttttgactt
    12361 cttcaaggat tttctctact gttttacctt cttttgctaa ttcagctgct cgaattacct
    12421 gaaaagataa tccttggtca gtggtatcac tgtcgataac cgttactttc gtttttgtta
    12481 gattactggc ttgtcttgct gcttctactg ttccactcaa gccttttgtc atatggatcg
    12541 aaatcacttc actgccgtct tctcctagct catcatacat cgagatgaat tctccaattg
    12601 gtggttggct tgttttgggc agattttttg ctttcgccat catttccata aatctttcgc
    12661 cgtctagttg gtcatcatcg ggatatactg ttccgtcgat catgatggat agcggcatga
    12721 cacgaatgcc taaagcatcg cgtacatttt tttccatcgt acatgaagaa tctgttacga
    12781 tttttacatt tgtcattatt ttctctctct ctttctttaa caagatgcct tattcatgtt
    12841 aaactacatg taagacaatt tctattttta gtataacaga caaataatga tttttcatta
    12901 attacaccta gtaaaggagc cgttttcatg gaaaaaacga aatttaatcg aacgtatgaa
    12961 atattaaatg aagtgtttaa tgcagtcacc catggaattg ggactggatt gagtatcgcg
    13021 ggactagtgg ttcttttagt aaaaggtgcc cacttgcatt cctccctcca tattgtctct
    13081 tatgctatct atggatcgat gatgattctt ttatttttaa cgtctacttt gtttcattca
    13141 ttgattttta caaaagcaaa aaaggtgttc caagtattcg atcatagttc gatttttctt
    13201 ttgattgcag gaagttatac tcctttttgt ttgctgagta tccgaggatg gctaggttgg
    13261 actttatttg tattaatctg gttgtttgcg attcttggca ttatatataa agcgttgacg
    13321 ttgcataaac gggagagagt atcaaaagta tctacgatca tctatatttt tatggggtgg
    13381 ctctgtgtcg tggctgctgt tcctctttat cactctcttg gaatgactgg tgttattttg
    13441 atggtagcag gcgggctttc ttacacgata ggcgcattct tttatagctt gaattctatc
    13501 cgctatatgc atgttgtttg gcatttgttt gttatgttag gtgcaggttt tatgtacttc
    13561 tctgttttat tctttactta ataaaaaaaa taaagctatg gatgggacag ctctaaataa
    13621 cagctagtct atccatagct tttttatatc gtcaattcgc ctgtcccgct ttttacagga
    13681 ttctcattac gctgatacgt ttcaaattga tgcggataac gatttttttc atccatttct
    13741 ccttgactag tattgatcag tgaccaatca gaccagtcaa caggtggaaa ataagcatct
    13801 ccatcgaata catgatggat gactgtacgg tataatatcg tacaaaatgg cagaaattct
    13861 tgataaacag ctgaccctcc agcgatgaaa aagataccgt ctgtttcttt ttccagagca
    13921 agcacgtctt ccacactatg catgacatgc acgccttctg ctttatagga agaatctctc
    13981 gtcaaaacaa tcgtctgacg attcggcaac ggacggctac ccatcccttc gaatgttttt
    14041 cgtcccatca ctagtgtatt tgcttctgtc atttgtttga agaatttcag atcatttggc
    14101 aaacgccaag gaagacggtc ttcttttcca atcaatccgt tttcatcctg tgcccaaatt
    14161 gcagctaaca ttttctcatc ctttctgatt ctaaacagcg ataggagctt tgatcgccgg
    14221 atgtgggtca tatccttcta attggatatc ttccatctca aaatcaaata ctgactgctt
    14281 ttcctgattc aaaactaaag tcggaaatgc gcgtatttct cggctcaatt gttctttcat
    14341 ttgttcgata tgattgatat aaagatgagc atctcctaat gtgtgaacaa attcgccaac
    14401 cgctaaacct gtttcatgtg caattaggtg agtcagcaac gcataacttg caatgttgaa
    14461 cggaacgcct agaaacacat ccccactgcg ttgatacaat tggcagctta gccgtccctc
    14521 ttgaacataa aattgaaaca tcgtatgaca aggaggcaaa gccatgctag gaacatcttc
    14581 cggattccat gcagaaacga tcaatcttct agaatcagga gtctttttga ttgcttcgat
    14641 cacttgtttg atttgatcga taaagccacc gtcttttgtt tcccagtgac gccattgata
    14701 gccgtagatg tgtccaagat cgccgtatgt ttcagcaaag gaatcatcgg ttaggatctt
    14761 ctcgcaaaaa agcttgctct gctttttata ttcttcattg aattcaggat ctgtcaaaca
    14821 tctatggcca aaatcagtca tatctggtcc attatagtct ggacttttga tgtagcgttc
    14881 aaatgcccat tcatcccaaa tatggttttt attttgtaat agatagcgga tattcgtatc
    14941 tccttttaaa aaccaaagca attcgctttt gatcaatcca aaaggtactc gtttcgttgt
    15001 taacaaagga aacccttttt gcagatcaaa ccgcatttgg tatccgaata agcttaatgt
    15061 gcctgtgcct gttctgtctt ctttacgatg cccttcttcc aacaattttc ttcctaagtc
    15121 aagatacgct tgttccatcc tttttcctcc taatcttcta cccattcact caagtattcc
    15181 caccgtgcca ttttttcttc aagttgtgct tctgtttcag atacgtcgtt ttgcaattct
    15241 tgtaatcgag tgaaattatc tccttgatga ttcatctctt cttggagcaa tgagattttt
    15301 tcttcaagtt ctgcaatctc atcttcaatc gtttcccatt ctttttgttc catgtaagat
    15361 aatttcttct tttcagcagg ttccttgctc tgtatttttg gtttcgcttt ttcagcaggc
    15421 tgctgggtct ctttttgctt cagtaaataa ctggacatgt ttccgtaata aagttcttgt
    15481 tttccgtttc cttggaatac taataactta tctgctacct tgtccaagaa ataacggtca
    15541 tgggaaacag tgatcacagc acctggaaaa cttctaaaat aatcttccaa aatcgtcaat
    15601 gttgctatgt ccaaatcatt ggtcggttca tccaataata aaacattagg ttgctgaatc
    15661 agcagtttca acaaaaataa ccggcgtttt tctccgcctg aaagtttgcc tatcagtgtc
    15721 ccatgcataa atcttggaaa taaaaatcgt tcaagtaatt ctgctactcc gatttgactg
    15781 ccatctgctg tttttgcttc ttcagcagct tcttgtaagt aagcaatcat ccgtttatct
    15841 ggtgccattt cttcattttc ttgtgtataa tacgcaagat gaaccgtttc accgatagac
    15901 attgtaccgc tatccagggg aattctgcca gctagaatat taagcaatgt cgattttccc
    15961 gcaccatttt taccagtaat acctagtctt tcccgagatt gaatcaataa atctaaatgt
    16021 tctagcagtg tttggtcatt tatacggtaa ctgccatctt tgatttccag tacttttttc
    16081 cctaaacgtt gtgtcgctag gttcagctct aaatcatctt cttggtttac ttggtacaag
    16141 ttctccttta gatcttcgaa acggttgatc cgtgcttgct gcttggtgcc acgtgcctgt
    16201 acgccagccc tcatccaagc taattcctgt ttataaagcc gcttgcgctt ttcttcttgt
    16261 tctacttcta cacgatcacg ttctgctttt tctaagacgt aagcttcata atttccttta
    16321 tactcgtata attttccaaa tgaaagttcg aagattcggt ttgctactct atctagaaaa
    16381 taacgatcgt gggtaaccat caatagtgcg ccacggtatt gtttcaaata gttttccaac
    16441 cactcgatcg cttcataatc taaatggttt gttggttcgt ctagtaatag taaatcgggt
    16501 tcatcaatca acacttgcgc cagactaact cgttttttct gaccacctga taaagtactg
    16561 atttttttat ctaattcact gattcctaat ttttgcaaaa taatctttgc gttggtatct
    16621 gtagtccaag catcttcttt attcatttgt tcttctgctt tagcataacg tttttgcaca
    16681 tcagggtctt ctccgttttc ccctagttcg atcaatgctt cctcatatgc tcgaacagtt
    16741 tggatcaagg ggctgtttcc ttgaaagact gcttggagaa cagttaattc atcggaaaat
    16801 tcagctgctt gagaaagata accaatacgg taatcagttg gataaaacac tgtttgccga
    16861 tcaccgtcgc tgctatcgat accggcaata atatttaaga gacttgtttt acccgtcccg
    16921 ttcgtcccaa tcaacccgat acggtctttt tcgtggacca aaaaagaaat ctggtcaaac
    16981 aaatcttttt caccataagt ttttttgagg tcagttactt ttaattcttt caacatgttc
    17041 cctccttatc ttggatattg tatcataaaa atagtgaact tcaggaaaat ttt
//
LOCUS       DACDPS010000050        17060 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.050, whole genome shotgun sequence.
ACCESSION   DACDPS010000050 DACDPS010000000
VERSION     DACDPS010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.050"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            252..743
                     /gene="nrdR"
                     /locus_tag="IWJ07_001809"
     CDS             252..743
                     /gene="nrdR"
                     /locus_tag="IWJ07_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAP9333387.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            766..2139
                     /locus_tag="IWJ07_001810"
     CDS             766..2139
                     /locus_tag="IWJ07_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAP9333388.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            2139..3065
                     /gene="dnaI"
                     /locus_tag="IWJ07_001811"
     CDS             2139..3065
                     /gene="dnaI"
                     /locus_tag="IWJ07_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAP9333389.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            3194..3634
                     /locus_tag="IWJ07_001812"
     CDS             3194..3634
                     /locus_tag="IWJ07_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP9333390.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            3778..4479
                     /locus_tag="IWJ07_001813"
     CDS             3778..4479
                     /locus_tag="IWJ07_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAP9333391.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            4476..5597
                     /locus_tag="IWJ07_001814"
     CDS             4476..5597
                     /locus_tag="IWJ07_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAP9333392.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            5612..6844
                     /gene="pepT"
                     /locus_tag="IWJ07_001815"
     CDS             5612..6844
                     /gene="pepT"
                     /locus_tag="IWJ07_001815"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAP9333393.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            6976..7884
                     /locus_tag="IWJ07_001816"
     CDS             6976..7884
                     /locus_tag="IWJ07_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAP9333394.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            7919..8191
                     /locus_tag="IWJ07_001817"
     CDS             7919..8191
                     /locus_tag="IWJ07_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAP9333395.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            8202..9248
                     /locus_tag="IWJ07_001818"
     CDS             8202..9248
                     /locus_tag="IWJ07_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAP9333396.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            9499..12108
                     /gene="clpB"
                     /locus_tag="IWJ07_001819"
     CDS             9499..12108
                     /gene="clpB"
                     /locus_tag="IWJ07_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAP9333397.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(12259..12930)
                     /locus_tag="IWJ07_001820"
     CDS             complement(12259..12930)
                     /locus_tag="IWJ07_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333398.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(12923..13417)
                     /locus_tag="IWJ07_001821"
     CDS             complement(12923..13417)
                     /locus_tag="IWJ07_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP9333399.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(13437..14657)
                     /locus_tag="IWJ07_001822"
     CDS             complement(13437..14657)
                     /locus_tag="IWJ07_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9333400.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            14804..16639
                     /gene="lepA"
                     /locus_tag="IWJ07_001823"
     CDS             14804..16639
                     /gene="lepA"
                     /locus_tag="IWJ07_001823"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAP9333401.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            16835..>17060
                     /locus_tag="IWJ07_001824"
     CDS             16835..>17060
                     /locus_tag="IWJ07_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9333402.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIG"
BASE COUNT         5718 a         3045 c         3596 g         4701 t
ORIGIN      
        1 tttttgtgct agatgactaa atcagctttt gtgtaagtta tttctcagta atcaaggtga
       61 tcagtattgg aaaattgaaa tttagtaagg acaggggaca caattacgca tgaaagtaga
      121 aaaaattgtt cttatatccg tgttttttgg gtaggacagt cgcgtttgtg atttgtttcg
      181 tgttataata caactagttg taaagaaaga cacatccttt atataaaatg cgatgcaaga
      241 ggtgagacaa aatgagatgt cccagatgtc aacataataa ttcacgagta atcgacagcc
      301 gtcaagcaga tgatggacgt gcgatccgtc gccgtcgcga atgtgagaat tgttcgttcc
      361 gattcactac atttgaacga attgaagctg ctccgttatt agtaatcaag aaaaacgggg
      421 aacgcgaaga gttcaatcgc gataaaattt taagaggact catccgttca gcagaaaaaa
      481 gacctgtagc tatggaacaa atggaacaaa tcgtcaacca tgtcgaaaac cgcgtacgca
      541 gtctcggaga aaacgaagta tcgacgaatc taattggtga atatgtgatg gaagatttag
      601 ttgatttaga cgaaatcgct tatattcgtt ttgcaagtgt ttatcgccag ttcaaagata
      661 tgtctgtttt cttaaaagag ttgcaagata tcgtggataa agcaaaagaa aataataaag
      721 agaataataa agaaaacaat taagaaacaa aaaggaggca tttccttgga aaatgcttgg
      781 aaagagcttc aaccaaaaaa tatttaccaa ggaagaaaag cggctccgct gagagaagac
      841 agcaagaacg ccttgctgta tctttatcag ccactaatcg gcggagaagc cttgagtatc
      901 tatttcaccc tgttatcaga aatctctcaa gaatctggca aaggtcctga agggatgcat
      961 gcagatctgc ttagtagtct aggctgcggt atcccgcaat tttatgatgc aagaaaaaag
     1021 ctagaaggaa tcggcttact ggatgtttat tttaaagaag atagtgatct gggacctcgt
     1081 tttatttatg agctgttaga accactcatt cctaaagcat ttttcaaaga ctctgttctg
     1141 gcttttcttc ttctggaaaa agttggtgaa cgacgattcc agaagcttac ccaattcttt
     1201 gaacctaaaa ggcatacagc agagggatat caaaaaatca caaaaaaatt tttagatgtt
     1261 tatcgcttca atgaagcagt gtacgctgct aatcaagaaa acatcgaatc gctgaataaa
     1321 tcttttgaag aatctgaatc agtagcatta cttgaagcag attcgacatt cgattggcag
     1381 tttttgaatg attggctggc aaaaaaacat attgcaagtc ttgatgatcc aatcatcaaa
     1441 caagtcgaca tgtatcatca gctttacggt ttcgatgaat tgacacttgg cgaactgatc
     1501 agtcaatctt atgactttag tgaacaaaaa gtatctgaaa aagaactgca aaaagccatt
     1561 ttggcaatgc agagacgtcc ggtgaagcaa acaaaagaaa cagttgcggt ttctgaaaac
     1621 agtaatgaac cctcgtcaaa cgaacatttg acaaacgcag cgcttcagct gataaaagaa
     1681 gcacagagca ttccaccgat gaaatatctg gaagcaatca agtcagaaaa aggcggatat
     1741 gtttctaaac aagaaaactg gttactgcaa gatcttgttt ctcaatcagg tctttccagt
     1801 agcgtgatca atgttttgct gaattatgta ctagtgatca agaataatgc tagcttaaat
     1861 gccagttatg taaatacagt ggcaaatgaa tgggcacaaa agaaaatcgc cacagcagaa
     1921 gaagcgatcc agcacattcg gcaaataagt aatcaagcaa aacaatcaaa acaaaagaag
     1981 caaacaaatt atcaaactgg caagcgaaat gttcgacggg aaaagcttcc tgattgggtc
     2041 aaccagccaa aagatgaaaa acagatcagc agcgagaaaa aagcagagat tgaccgccgt
     2101 tttaaagaat acctggcaaa aaaggagggt gatatctagt ggaagacgta ggcaaagaat
     2161 tgaagaggat catcagcaaa cgcaattatc aagaaaagta tgctgaaatg atcgctgaag
     2221 tcttaaatga tgcggatgtt caagcttttc taagcgcaca ccaagaagaa ttgacacaag
     2281 cggatattga aaaaagttac gctaaattgt atgaattcgt gcaagaaaag cgtaaatatc
     2341 aaaaaaacga tccaacgatg atcgctcccg gctatgagcc gaagctgacg ttgaattttc
     2401 attatgtgga tgtcacttat gtaccaacgg aagcattgtt agcacggcaa agacaagaag
     2461 aaatcagaag ccgagtaaaa gcaatggata tgccaaaaga tattcaatcg gcttcttttg
     2521 aaaactttga acgtacagat ggacgaggat atgcgagctt agaagcccta gattttgtag
     2581 agaaatatac tgctgatcca aaagggtatc ataaaggact gtatctagta ggaagttttg
     2641 gtattggaaa aacatattta ttaggagcaa cagcgagaga attagcgatt gctggattca
     2701 ccacgacgct agtccatttt ccgacatttg ccgttgagat gaaacaggca atcggaaaag
     2761 atcaagtggc agaaaagtta gatgcagtga aacgttcacc tattttgatg atcgatgaca
     2821 ttggtgcaga tgcgatgagt agttggatac gagacgaagt attcggtgtt atccttcaat
     2881 atcggatgca ggaacagtta ccaaccttct tctcatcgaa ttttacaatg gatgaattgg
     2941 aacagcattt gtccgtcact caacgtggag atgaagagcc gctaaaagcg aaacggataa
     3001 tggaacgcat acgttatttg acaaaagaaa tcgaaatgac tggtcgcaac cgacgcaatg
     3061 gttgaacagt tgacaagtcc aactgacatt tgacctagta gtaaaagcag gtcagcgaat
     3121 cattatatgg acagaaatga ccggagacaa tcaccgcaac aagtaatgca gaaacttaaa
     3181 cggaggattt tttatgaggg ctattttttc tagtatatct gtagttggtc tattttttct
     3241 tttagcaggc tgcggtagcg cagatagcaa cacaggttct gttgattcga ttgatgcggg
     3301 tcaaacagta aaaacaagca gtacgaaaac ggagagacaa agcatatttg tcggaacgat
     3361 tgaaagtatt actcaagtag aagcagaaga agaaacgctt cagttgaaac tcgtcgatgt
     3421 catggccaaa gaagatccag cgaatatggg aacgtcattc agcaatgacg gagtgatctt
     3481 aaatgctact gctgaaaaaa taagcgtgga cataaatgaa ttgaaaatcg gtgacaaagt
     3541 ccaatttaca ttagttgaga tgccgatcat gacgatgtct atcccaccgc aagttcctgg
     3601 agattcgatt attgatgttt cattgaacaa ataaccaata acattttttc aaaaataaga
     3661 aaatgttgtt ttatagaacg aaaaggtata ttttttaagg agatatggga atttttttat
     3721 tcctagtctc cttattttat ttggaaaatg ctataattat ctagaaggag tgaagctatg
     3781 aaccatttag atttatctca gcgcttagcc gcagtgggtg actttgtccc tgaatccgct
     3841 cgtttagcag acattggatc agatcacgct tacttacccg ttgcattgat attaagagga
     3901 aaaatcgaat atgcagttgc tggtgaagtc gttcaaggtc cttatgaatc agcacgtaag
     3961 caagtcaaaa aagatggatt agaagaccgt atcaccgtac gccttgcaaa tggtctagat
     4021 gctatccagc cacaagatgg tattacagct gtcacgattg cgggtatggg aggttcactg
     4081 atccgagata tcctagaagc tggcaagttg aatcaacgct taagcggaca agaacgtttg
     4141 atccttcagc caaacatcgg agagcgtaca ttacgatctt ggctgcaaga aaatcagtat
     4201 aaaatccaat cagaaaaaat acttgaagaa aataaaaaaa tatatgaaat cattgtagcc
     4261 gaaaaagaac aaaaaccagt tacttacaat gaaatggagc tgatgttcgg gccatttctg
     4321 cttgaagaga aaagtgagat cttcaaatca aaatggacaa gagagcttca acaaagaaaa
     4381 gctatccgtg aacagctcaa acaagcagct aatcaagagg cacgtcaaaa acaaatcgag
     4441 caggaaatcc aaatgattga ggaggtttta aagccatgac aatcagcgga cgcactttca
     4501 tccaaaagtt tgaaagctat tgtcctcagt ggctagctga agaaggagac ccagtcggac
     4561 tgcatatcgg aacattggat aagccaatcc aacgagtaat gatgacatta gacgttcgtc
     4621 cagaagtggt cgaagaagca atcaagaaaa aaatcgattt gctgattgct aaacatcctc
     4681 caatctttcg tcctgtaaaa cggctggtta ctgatcagcc acaagaaaaa atgtatgcag
     4741 atttgttgaa acatgatatt gccgtctatg cagcacacac gaacatggat attatttggg
     4801 atggactgaa tgactggttt tgtgaacttc tcggtattga agtggagagt tatttagtaa
     4861 agactcatga gattcattat aaaaaactgg ccgtttatgt acctgtggat catgcacaga
     4921 aaatgcgtga agtattagct gcagctggtg ctggtacaca aggtgattat accggtacaa
     4981 gttttacctc gattggacac ggtcgattta ctccggaagc tggtgcacaa ccagcgattg
     5041 ggaaagtagg gaagacagaa caagttcaag aggcaaaagt tgaggttatt ttgccggaaa
     5101 ctatcgaaaa acaagtgatt caagccatgc ggtcagctca tccttatgag gaacctgctt
     5161 atgatctttt cgctatagat gaaccagtcg aaatgtttgg gttaggacga gtaggtgaat
     5221 tgccgcaaga aatttctata gaggcatttg tagaacaagt aaaagaagcg ttccaactag
     5281 atggactgag aatagtccag ccaaaaaatg caaaatcctc tgtgaagcgc attgcgattt
     5341 gcggtggaag cggtgaaaaa ttttatccgc aagcaatcgc ccaaagagca gatgtctata
     5401 tcacgggaga tatctattat catacggctc atgatatgca aagcgctggg ttgattgcaa
     5461 ttgatcccgg tcattatatt gaatcgttat gcaagcaacg atttatcgag aaatttgaat
     5521 cttggaaaca agaagaaaat tgggatatta actttttcgt ttctgaaaca aatacaaacc
     5581 catttcaatt taattaaaag gagagaatac tatgtacgaa aatcttttgc cacgtttttt
     5641 acgatatgtc aaaacagaaa cacgctccga tgcaacaagc aaaacgactc catcgactca
     5701 gacacaagtt gcttttgcgc aagaattgaa aaaagaatta gaagagttag gcttagctga
     5761 tgttttctat aatgaagata acggattcgt tatgggaaca ttaccaagca atgttgatca
     5821 tgaagtgcgt tccattggat ttatttccca catggataca gcagatttta atgcagtagg
     5881 agtcaatcca caaatcattg aaaactatga tggcgagtct acgatcgtac tagatgcgga
     5941 agaacgctat acattgaata caaaagattt tccaaatttg aaaaattatg aaggccacac
     6001 attgatcaca acagatggca gtacattact gggagcagat gacaaatcag ggattgcgga
     6061 aatcatgact gcaatggaga tcttgatcaa taatccttct atccctcacg gtaccatcaa
     6121 agtggctttt ggtcctgatg aagaaatcgg tgtcggagcg gataaattcg atgttgagca
     6181 attcgatgta gattttgctt atacagtcga tggtggtcct ttaggtgagt tgcagtatga
     6241 aacgtttagt gctgctcaag caaatattac gatccaaggg aaaaatgtac atccagggac
     6301 ggcaaaagat acaatgatca atgctctaca gttagcaatc gattttcata atcagcttcc
     6361 agccgatgaa gttccagaaa aaacggaagg atacgaaggt ttcttccatt tgatggcatt
     6421 ggatgggaat cctgaagaag cttctatgag ttatatcatt cgcgatcatg atcgtgaaaa
     6481 attcgaagca cgcaaagcac aaatcacaca tatccaagaa gagttgaatc agcgatttga
     6541 tcaagaacga atcaaagtag atatgtatga tcaatattac aacatgaaag aaatcattga
     6601 aaaagatatg agcatcgtgg aattagcgaa acaagcaatg atcgagcttg gtatctctcc
     6661 agtgatcgaa ccagttcgtg gaggaacaga tggttcaaaa atctcttaca tgggtattcc
     6721 gacgccaaat ctatttgccg gaggagaaaa tatgcacgga cgattcgaat ttgtttcttt
     6781 acaagtaatg gaaaaagcaa cagacgtgat tgtaaaaatt gcagaattga acacaaagag
     6841 ttaaaatcga aaaagaacag cttatgacaa ggcgttcgtc ccaatgatgg aatgtcatct
     6901 gtctaaggct aaagggcagg acaatggaat tggttgtccc gccctctttt ttgaagagta
     6961 aataggaggt aaagaatgga aaagctgcta gttttttata acaaaagttc agggaaagat
     7021 gagggtgaga aattagcacg ctggtttcaa gattatgtaa aagaaaagca gccagaatta
     7081 tatgtagaac tagctgaaac tggcccatcg attgatgatg cggaattagt caagaaagca
     7141 gaagccatag aagctgatac tttagtaatt ataggtggag acggcacgat tcatcatatc
     7201 gtccaagcgt tccaaaaaac attatctcaa taccatgtcg gattacttcc aggtggaact
     7261 gtcaacaacc ttgcacgtgt tcttgagatt cccctcaaaa aagaagaagc tgctgacgtg
     7321 atcctggaag gcaatgtacg gaaaatagac tatggtcaag tcaatgatga agtcattatc
     7381 tctacactga caatcggaat cttagcagat acagcagttt ggatcagtca agaagaaaaa
     7441 cagcgttacg gctcgtggat ctttatcaag aagtttttta agttattgct gaagaagaaa
     7501 aaatatcatt tggatttaga aacagacgag gagcattgga aaggaaaatc ccaactgttg
     7561 acagtcacga tgtctaactc cgtgggcggt tttacaaatt ttgacgactc agcgacccta
     7621 gatgatggaa aatttcatgt aacgatcgta ccttccttag atattttccg atttattttt
     7681 tatttgccta gaatcatgag aggcaaattc tatacgatcc ccggcattac ttatttcaca
     7741 gccaaacgca tgaaaatcca agcgcaggaa aaatctgtgg gcactcgaac ggatggagac
     7801 acgacagatg atcttccagt tgaattatct gttgtatcaa aaggattaac agtttattca
     7861 ccaaagaaag aagaaaaagc ttagaaatag tagttaacag aaagttggaa gaagagtaat
     7921 ggatgtattg ttacacaatc ttttgtggtt ttaccttttc attgtgaatt tttacttatt
     7981 ttgtctgatg gggtatgata aacatcaggc tagaactagc ggctggcgtg ttcctgagaa
     8041 taatttgata tttgtcagcc tgttaggcgg tggtttggga ggattgatct ctcgaaaagt
     8101 tttccatcat aaaactcgga aaaagaagtt tactatagga ttcgctgcag gtgtacttgt
     8161 tgatttgatt ctcatttatt ttttccatta aggagcagga gatgtcgtct aaaggaacga
     8221 aaactaaaat cattctgaat ctgcttttgc tgattgtgat ttttgccatt atttattatt
     8281 taatcaatca atcatttgca gatatctttg ccgaattgct gtctaccagt gttgttgttt
     8341 tggcagcggc tgttatatta gggacagttt accaaatcgc tgaagggcgc tcaatcaaag
     8401 aaattgctga acctttcgcg ccgaatttta caacgaaaga tgggttcttg acctctagct
     8461 atattgcgtt ttaccgaatc gtttcgtttg gtacaggaac gctgatctcg gagatttatt
     8521 tttataacaa aaaaggaatg aagctatccc agggagcagg ggtgacagca ctccatatga
     8581 ttatgtacaa actggcagtg atcacttatg cattagctgg cttggtcatc caattctctc
     8641 ttttttattc taatggtcca tcaatgatct ggtttatcat cgctggtatc cttttgacga
     8701 tcgtgattgt tgccttttta ttggcgatgt ccgtcagctt gaatgttcaa atattttttg
     8761 tcagcttatc gaataagtta tttaaaagtg agcgtttgcg gaaatgggtg gatacatgca
     8821 atcagcagat ctactcttta agggaaacgg tccaaaccat tatccaagac aggactgcgc
     8881 tattgcggat ttatctatgg aatatgttaa aacttgtatt ttggtatgta cttccttatt
     8941 tgattctgat tgaaaaccat cctaaaatag attttctact gaccatttcg tttatcagct
     9001 ttgcagtagt gctttcaggg gttattccta cgcctgctgg tattggttcc tttgaatttg
     9061 tctatttgtt attattcaga ccagttgttg gaacagttga cgcagtttct tcgcttttac
     9121 tgtatcgttt cagttccttt atcttgccat ttctctatgg atttgtgtat gtgattgctg
     9181 aacggcgaag agtgatcaaa gaagaaataa tagaagtaaa aaaagagaat aaagaaaatc
     9241 aagggtaggt cttttataag atctaccctt tttatcaaat caaagaaaaa cgttatggat
     9301 aagataattt tacgtttgtt tggctctttt tttatataca tacaatcaat tttgtttttt
     9361 taagatttat ataagtcgag aaaaaaagtc aaaaaaccat gtgtatattt tgaccaaaat
     9421 tgaccaaagc gatataatag tttttgtaaa gatcaaagaa ataagtgatc ttacctttaa
     9481 ttaggagggt atacctgtat gaatattgag aaaatgacta cgacgcttca agaagcgatt
     9541 gctgaagctc aacagattgc ggtcactcga aaacatcaag atatcgacat tgcccatgta
     9601 tggaaaatct ttttacaacc taatcatttt ggtcgaaatt tctatacgga tgccggattg
     9661 gatgtcgaat cttttgaaca tgaaattgat cgtctactgg atgagtatcc agttgtttct
     9721 ggtggaaacg tccaatatgg acaaaacctc agccagaatt tctttcgatt attgaatgaa
     9781 gcagaccaaa tacgtgaaag cttcggcgac gagtttcttt caacggaagt cgttatttta
     9841 gcgttgatga aattaaaaaa ttatccgtta actgtttatt tgaataaaaa cggattatct
     9901 gaaaaagaat tgcgtaaaaa catcgaagag atgagaggag gagaaagagt gacctcacaa
     9961 aaccaagaag aacaatacaa agcattagaa aaatacggcg ttgatttagt acagcaagtc
    10021 aaaagcggaa aaatggatcc aatcatcggt cgtgatgaag aaatccgtga tgtcatccgt
    10081 attctttcac gaaaaacaaa aaataatcct gttctgatag gagaacccgg tgtagggaaa
    10141 acggcaatcg ttgaagggct tgctcaacgt atcgtacgca aagatgtgcc tgaaaattta
    10201 aaagataaaa cgattttttc attagatatg ggtgcattga tcgctggagc aaaattccgt
    10261 ggtgaatttg aagaaagact aaaagcagtc ttgaaagaag tcaaaaaaag tgatggacgt
    10321 atcctcttgt tcatcgatga aatccacaat atcgtagggg caggaaaaac agaaggaagt
    10381 atggatgccg gaaatctgtt gaaaccaatg cttgctcgcg gtgaattaca tttgatcggt
    10441 gccacaactt tagatgagta ccgtcaatat atggaaaaag acaaagcgtt ggaacgtcgc
    10501 tttcaaaagg tattagtcaa agaaccaaca gtggaagata cgatcagtat tctgcgtgga
    10561 ttaaaagaac gttttgagat ccaccatggt gttaacatcc atgacaacgc attagtagca
    10621 gcagctactt tatctgaccg ttatattact gatcggttct taccggacaa agcaatcgat
    10681 ttgatcgatg aagccagtgc aacgattcgg gtagaaatga actctatgcc aacggaatta
    10741 gatcaagtta ctagacgttt gatgcagttg gaaatcgaag aagctgcact gaaaaaagaa
    10801 tcagatgatg caagtaaaaa acgcttgaaa aatttgcaag aagaattagc tgaacttcgt
    10861 gaagaagcaa atgcgatgaa gatgcagtgg gaaacagaaa aagaagaagt caattctgtt
    10921 tcagcaaaac gtgctgaaat cgataaagca aaacacgaat tagaagatgc agaaaataac
    10981 tatgatttag aacgcgctgc tgtattgcgt catggtacga tacctcaatt agaaaaagaa
    11041 ttgaaggaat tagaagctaa agcaaaagac agcgagatta agatggttca agagtccgtt
    11101 accgaaaatg agattgctca agtcgttggc agattaacgg gtatccccgt aacgaaactt
    11161 gtagaaggtg aacgagaaaa actaattaaa ttgaatgaaa cacttcataa acgagtgatt
    11221 ggtcaagatg aagcagttga tgcggtcagt gacgcggtaa ttcgttcacg ggctggattg
    11281 caagacccaa atcgtcctct aggttctttc cttttcctag gaccaactgg tgtcggaaaa
    11341 accgaattag cgaaagcgtt agctgagaac ttgtttgact cagaagatca tatggtacgg
    11401 atcgatatga gtgaatatat ggaaaaacat gctgtgtctc gtctagtcgg agctcctcca
    11461 ggctatgtag gttacgaaga aggtggtcaa ttaacggaag ctgtcagacg aaatccttat
    11521 acaatcgtct tgttggatga aatcgaaaaa gcgcatcccg atgtcttcaa tatcttgtta
    11581 caagtcctag atgacggacg cttgactgat tcaaaagggc gagtagtaga tttcaaaaat
    11641 acggttctga tcatgaccag caatatcggt tcacagttac tgctggaagg cgtgactgct
    11701 gatggaacga ttccagaagc agtggctgaa caagtcaaca cgctattacg tggaaacttc
    11761 aaaccagaat tcttgaaccg aatcgacgac acgattttat ttacaccatt gagcctagac
    11821 aacgtgaaag gaatcgttga caaaatggtg gcgcaattgg cacaacgtct ggaacatcaa
    11881 gaaatcctgc tgacaatcag tgatgaagcc aaaacgtgga ttgctgaaaa tgcttatgaa
    11941 ccagcatatg gtgcaagacc attgaaacga tttatcacta aggaagtaga aacaccacta
    12001 gctaaagaaa tcgttgcagg acatgttatg cctaaatcaa aagtaacgat tactttactt
    12061 gatggacaac ttcatttcaa aacagaagag ctagaagaaa tcgtctaatc ttccattgag
    12121 actataaaag aaaaaagggg tctgacacaa ctagtgtcag acctcctttt ttcaaataaa
    12181 cagtgttcca gaagtagttt cttcgaaaaa taagccgaaa tttcttgaag ctgcccgctt
    12241 ctgtcacaac ctcttttatt aggtattttt cactttttct aatttataac tttcaaagta
    12301 acgctcatct tttttacttt gagaccatgc aaacaatccc attaaggcag cacctatcgt
    12361 atttgtcagc aagtcgccca tcgtatccat caatgctgct cgtccgacaa acagtgtgcc
    12421 atcagaagca gcaaaacgtt gaagattcat gcctaagaac tggtcgcata agaattccca
    12481 gaactcccaa aacacgccgc aaagtccagc aaacgcaaag ccgaataaga ggaacaacca
    12541 aggactggtt tttgatatct cagcatcttt catcaagtaa ccaatcaatc catagccaag
    12601 agctgtcagg accattggac tcaccgcatg taagattttg tcccaaaaag agatgatact
    12661 gatcaaatgc atccctgtgc ctaaaaagac agagatgaac aagaaaaacc agtaaaacaa
    12721 aacgattgct gctggtattt taagtttgaa aactttcaaa atgacttgtg gcatgaaaat
    12781 caatgcaatc cctaataaag attccacgat aaatgtgatt ccttgagaag tcgaatattt
    12841 ttgtgtgata aattcataaa aattatagca gacaacgaat acaccaaaag caagtaaagc
    12901 tatttgcacc tttttgattt ctttaatcac tgaaagtccc tccatctaaa aatcttttta
    12961 cttctgaaag caactcaatt tctgaagaaa ccacttttcc gttcaatttg atcaaaccta
    13021 ttgtatataa gttcaggtag tgaaattgat tttcagctgt ttcttgtagt gcagcgattt
    13081 ttttcgggtg ggttccgcct tgctgtcgag tatcagtata taagccgatc actgggatgc
    13141 ctttcgcata agccacaccg atttccgatg ctaccccggc atcgatcgtc agcccatcta
    13201 gtaaagcaat cattaaatca ctagccaaca ctttttctgt gtcagcaaga gcaatcattc
    13261 gactgtctgc ataagcagtt ttgtcattga ttgcttcatt ttcttgagga agatagatcg
    13321 aaagctcaga agagagctct ctgatttctt taactaactg ttggttatat aacaaatcac
    13381 ttttagcaaa cattggtgcg gcaaaataaa ttttcatcta aacatcatcc tttttatcaa
    13441 aaataatcgc taaagtaagc ttccatgttt ggaatgatac gctctagtga cttcagtgtt
    13501 tcttcatctg tttctaaccg ttcgattctt gccaaataag aagcacggcg ataattcatc
    13561 atcaagcaag tcaatgtttg gatatccagt cgaacggatt ttcctatagg ttcatttaag
    13621 acacggactt gtccctgttc atcccatgtt aaagcgaaga caccattatt ccaaggagcg
    13681 acaggatctt caatgatgaa atgaaaagca tcagctgttc caacaaacgg aaaccgttga
    13741 aggaattctt ttacatcaac gatccgagcc ataaaatatg gttcgatctg ctctttgatc
    13801 tgactatctt caatcaaaaa tgctaaaggc tcatttttat aaatatctcc atgtacccag
    13861 tagatcatgg aaaaatgtgc ggaaataaag ttccataagc catttcgagc ttcttgatca
    13921 agatagaaca tttcttttac gtgaaatatt tcttcagcta cccagtagaa cagaacacct
    13981 cttggttcat ggttgctatc gtagtagaca gcggctgtgc gctcatcttc attttcgaaa
    14041 cgccaatact cttcccaatg aaaatcactg cggaataaag caccatggtt ttgttgggca
    14101 aatttgtcat aaacggaaaa tacgtctgga tgatcaactg gtaatcgctc aaccatacca
    14161 cttactggga tttgtttagg tagctgtgta tctctgattt taaaggaaag tttatcagac
    14221 atgatttccc atcctttacg ccgatagtag gggatactgt aaggatacaa ataagagatc
    14281 cattgcttat tcttgcgcat attatccaat gcaacgataa ttagatcttg catcaatcca
    14341 tggttagcat attctggata agttccaact ccagtcacac cacccatttt ataacgtgca
    14401 ccgtgaatat tgacttcaca aggatagata gcgatttgag agatcagttt attttcgtgg
    14461 aaccatccga acactttcga tacttccaaa atcggttttt tggaacggat aaaagctcgt
    14521 ttattctcaa acccgctttc ctcgatgtct gcttcagtca cttggaacac ataggaaagc
    14581 aattcattga attgatcgat atgttcttga gtgaccggtt ttaattcaag attttctcta
    14641 aaatcttttt gatccatttt ttcatctcca tttattagct tgataacagt atagcaaaat
    14701 tctacaaaaa aaacttctat agctgactaa ttaaaaactc tttaaattac tgcttttcgt
    14761 tggtataatg gaaagtgctg attatatgaa aagaggagac gctatggata taaagaaaat
    14821 gaaagaacgc caagagcaga ttcgtaattt ttcgatcatc gctcatatcg accatgggaa
    14881 atctacattg gccgatcgta ttttagaaat gacgcacact gtgacgtctc gcgaaatgca
    14941 agatcagctg ttagactcga tggatttgga acgtgaacgt ggaatcacga ttaaattaaa
    15001 tgctgtagaa ctcaattaca cagcgaaaaa tggagaaaca tatatctttc acttgattga
    15061 caccccaggg cacgtcgact tcacctatga agtctctcga agccttgctg cctgtgaggg
    15121 tgccgtttta gtggtcgatg cagcacaagg aatcgaagca caaacattgg ccaatgttta
    15181 tttagcgtta gacaatgatt tggagatcct gccagtcatc aataaaatcg acctacccgc
    15241 agcagatccc gaacgtgtcc gtcaagaaat cgaagacgta atcggaatcg atgctagtga
    15301 agctgtatta gccagtgcca aatcagggat tgggattgaa gatattctcg agcagatcgt
    15361 ggaatatgtt cctgcaccaa gtggagactt agatgcaccg ctaaaagctt tgatctttga
    15421 ctctatttat gatagttacc gcggtgttgt tctaaatgtc cggatcacgg acggtatggt
    15481 caaacccgga gacaaaatca aactgatgag taatggcaaa acatttgatg ttacagaggt
    15541 cggggtcttc tcgccaaaag cagttgctag agactttttg atggtcggtg atgtcggtta
    15601 tatcacagca agtatcaaaa ctgtccaaga tacacgagta ggggatacag taacgttagc
    15661 agacaatcct gccgctgaag ctttaccagg ttaccgaaaa atgaatccga tggtttattg
    15721 tggtctttat ccaattgata cttctcgtta caacgatttg cgggaagctt tagaaaaact
    15781 gcaattgaat gatgctgctt tacaattcga accagaaacc tctcaagcat taggttttgg
    15841 gttccgctgt ggattcctcg gacttcttca tatggatgtc gtccaagaac gcttggaacg
    15901 ggaattcaat ctagaattga tcacgactgc accatccgtt atttaccatg ttaataaaac
    15961 ggatggtacg actgctacag tagataaccc ggcagatttt cctgaacctg taacgattca
    16021 ggatgtagaa gaaccattcg tcaaagcaca gatcatggtc ccaaatgatt ttgtcggtgc
    16081 agtaatggaa ctttctcaac gaaaacgggg agaattcatt acgatggatt atttagatga
    16141 ttaccgagta aatgtcgtct ataatattcc gctttctgaa attgtctttg actttttcga
    16201 caaattgaaa tcaagcacga aaggctatgc ctcattggat tatgaaatgt ctggttatca
    16261 aaaaagcaag ttagtgaaga tggacatctt gctgaatgga gaaaaagtcg atgcattaag
    16321 ctttatcgtt caccgagact ttgcctatga acgtggaaaa gcaatcgttg aaaaactgaa
    16381 aaaactaatc ccacgccaac aatttgaggt gccaatccaa gcagctattg gacaaaaaat
    16441 cgttgcccgt tcagacatca aagccttgcg taaaaacgtc ttggctaaat gttatggtgg
    16501 agatgtttct cgtaaacgta aactcttaga gaaacaaaaa gaagggaaga agcggatgaa
    16561 acaaatcggc tccgtcgaag ttccgcaaga agccttcatg gccgttctga aaatggatga
    16621 gcaagatagt gtgaaataac ttataatcaa tcgaaaaacc tattaaatca acctttttga
    16681 gtagatgcaa cactgaggat tatctgtata aaagatttta aactgtcatt tggttatcac
    16741 ggcgatacta aaattaacaa aattggaata tttttcttga agatttttag ctgtcagtat
    16801 atgatgaaag catctcatag gaggtgcttt tttcatggtt gataattcaa gtggaagggt
    16861 tcttaaatct ctaagaaaag agaaaaaact atctcaaaaa aagttagctg atttagcagg
    16921 tatttcacaa agtactttag ttaaatatga aaaagggtca agaaaaatcc ctaaagacgt
    16981 agataatact ctaagcaaga ttctgaatat agaaactcta ataaaagatg aggaagataa
    17041 gattgagatt ctcatcggtc
//
LOCUS       DACDPS010000051        16633 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.051, whole genome shotgun sequence.
ACCESSION   DACDPS010000051 DACDPS010000000
VERSION     DACDPS010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.051"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..167)
                     /locus_tag="IWJ07_001825"
     CDS             complement(<1..167)
                     /locus_tag="IWJ07_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP9333403.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLV"
     gene            complement(169..366)
                     /locus_tag="IWJ07_001826"
     CDS             complement(169..366)
                     /locus_tag="IWJ07_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAP9333404.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(760..2487)
                     /locus_tag="IWJ07_001827"
     CDS             complement(760..2487)
                     /locus_tag="IWJ07_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9333405.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2480..3673)
                     /locus_tag="IWJ07_001828"
     CDS             complement(2480..3673)
                     /locus_tag="IWJ07_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9333406.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3860..4504)
                     /locus_tag="IWJ07_001829"
     CDS             complement(3860..4504)
                     /locus_tag="IWJ07_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9333407.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4598..4732)
                     /locus_tag="IWJ07_001830"
     CDS             complement(4598..4732)
                     /locus_tag="IWJ07_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAP9333408.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4734..6866)
                     /gene="feoB"
                     /locus_tag="IWJ07_001831"
     CDS             complement(4734..6866)
                     /gene="feoB"
                     /locus_tag="IWJ07_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAP9333409.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6863..7336)
                     /locus_tag="IWJ07_001832"
     CDS             complement(6863..7336)
                     /locus_tag="IWJ07_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAP9333410.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7737..7961
                     /gene="nrdH"
                     /locus_tag="IWJ07_001833"
     CDS             7737..7961
                     /gene="nrdH"
                     /locus_tag="IWJ07_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAP9333411.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8120..10279
                     /gene="nrdE"
                     /locus_tag="IWJ07_001834"
     CDS             8120..10279
                     /gene="nrdE"
                     /locus_tag="IWJ07_001834"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAP9333412.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10329..11294
                     /gene="nrdF"
                     /locus_tag="IWJ07_001835"
     CDS             10329..11294
                     /gene="nrdF"
                     /locus_tag="IWJ07_001835"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAP9333413.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11383..12522)
                     /locus_tag="IWJ07_001836"
     CDS             complement(11383..12522)
                     /locus_tag="IWJ07_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9333414.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12831..13544)
                     /locus_tag="IWJ07_001837"
     CDS             complement(12831..13544)
                     /locus_tag="IWJ07_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAP9333415.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13798..14499)
                     /gene="nagB"
                     /locus_tag="IWJ07_001838"
     CDS             complement(13798..14499)
                     /gene="nagB"
                     /locus_tag="IWJ07_001838"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAP9333416.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14733..16265
                     /locus_tag="IWJ07_001839"
     CDS             14733..16265
                     /locus_tag="IWJ07_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9333417.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16456..>16633)
                     /locus_tag="IWJ07_001840"
     CDS             complement(16456..>16633)
                     /locus_tag="IWJ07_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9333418.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5161 a         3428 c         2875 g         5169 t
ORIGIN      
        1 ttcaccaatc tatttattaa aggactggga tctaattcat atttttgttt ttctaatttt
       61 aaatatacct tttgtaaaac ctcaagaata tcagaatatt taggatcttt acttatagat
      121 atatcattat ataattcatg aacaatttgc ttagcatttt ttttcatttt agcacttccc
      181 tggaattgct ccacctaaaa aaccacctat agacattcct gcaatacaag tagttgcctt
      241 ggcccaatcg accgtacatt tatttttagt gcaatacact ccatttccat aatattttcc
      301 actatgagtg gtacccccat agataagttg tgtctcttta atagacaaaa tttttaaatg
      361 tttcataatc taatcacccc taaattaatg tttttagact attattctaa cattcattaa
      421 atttcaaaaa acaattcctt cttgaaacgt agcaaaataa ttcctgaata agaatttaat
      481 cacttttaag tatataaact atgtatattc tttaatattt tttgagaatg cacttcgtac
      541 ataataaact ttaatcacat tagtctttca tacctacgat cgatctcttt gactcgcacc
      601 cataaattca taatagtttg tcggcgtctt tgaacgtttc gaagatgagc tacgcattct
      661 ctttttttat agacaaaaaa gatagagacg aacatggagc ttccaaattt ggatatttga
      721 tccaattttg gaagctccat ttatttcgtg tctgtctttt tattttttcc agctttcgac
      781 taattcttgg ttctctttga tccagtcttt tgctgcttgc tgtggatctt ttccattgtt
      841 gatttccaac ataacttttt ccatatcttt ttctgaccaa tggaaattgt ctaatacttt
      901 gtaagcttct ggctggtctt ctttcaatcc ttttcgaacc atcgtatgga tctgctcttc
      961 gcttcccatt ccgttttcag gatcttctaa gtatttcaga tcatatttgg caaacatcca
     1021 atgaggtgtc catcctgtaa ctacaattgg ttcgtgtttt ttgatcgctt gacctagtgc
     1081 aacggtcatt gctcctgaag aagatgtctc tactttccag tctttcaagt tgtcatattt
     1141 ttggatcgtg ttttcagcag ccgtaactac accagctcct ggctcgattc ctatgatttt
     1201 cttacctgcc tgatcggtta actcatcgat cgaatttaca tccatgtagg ctggcactgc
     1261 taaaccaact tttgctcctg tcaaattggc acctaggtct tcgacttgat ctttatattg
     1321 tgcatattgt gaaccatgag ttttaggtag ccaagctgaa accatggcat ctgactcgcc
     1381 atttgaaaca gatttccaca tgattgaatt atctaatggc gtcatcgtta catcatagcc
     1441 catttgtttc aatacttcac cgactacatt tgttgaagca acttctgtat cccattcaac
     1501 ataagataag ttgatacgtt ttgtttcttt ggcagatgaa aaactcccta taccaatcgc
     1561 acctaagatc actactacta cagcaccaat gatcagtcct tgacgttttt tacttttctg
     1621 tgttgttgct cctgcagctt ttttattgtt tggctggttc aaatgctgtg taaaacggtc
     1681 aatgatgatt gctaagataa ctaatgcaac cccgttgaca aatccattcc cgacttgtgc
     1741 tctttgcaat gctgataata cgccgcgtcc taagccgggt gcaccgatca tagaggcaat
     1801 aacgaccatt gaaagtgaca gcattgttgt ttgattgatc cctgccatga tcgtactttt
     1861 agctaaaggc aattctagtt tgaatagttt ttgacgactt gtgctcccga atgaatcaga
     1921 agcttctact aattctttag gaacttgtcg aatccctaaa ttcgtaaaac gaacggttgg
     1981 tggcagagca aagatgactg aggcaaatac ccctggaacc atcccgatac cgaaaaatgc
     2041 aactgctgga atcaaataaa cgaatcctgg cattgtctgc ataaagtcaa ggatcggttt
     2101 gataatactt tgggctttac tgctttttgc catcaagatc cctaatggta ccccaatgat
     2161 gatcgagatc acactagata aaagaacaag tgtgacagta ttcatcaaat cattccatag
     2221 accttggtta taaataaacc atagaccgat taatgtgaac aacgtaagtc ctggacgttt
     2281 cttagaaata aagaaagctg ctaccgtcaa taacaagata aacaaaggag ctggaatcac
     2341 taacagtaaa tttgtaatcc ccgacatcac tgcctgtcca atcgtttgta aaaatgaaaa
     2401 taggccagaa aaagttgtcg taaaccattc tgtgatattt tctacccagt ttgccactgg
     2461 taattgatag ttcaatagat tattcatgtt cactcacctc gcttgtttct gctaatgctt
     2521 caatcacact acctcggatg acgacacctt ttaattttcc atcctctaca actgcaagtg
     2581 gagtcggtga gtcataaata atattgaaaa tatcacttac caccatgtct ttcccaatca
     2641 ccgtcacatt ttcatctagg aagtcgatca atggacgttt ttgcttccta gcttctaacg
     2701 cttgatctgc agtgatgatc cctttcaggt gtctctttct gtcaacagcc atcagcatgc
     2761 tgacttcttc attacgcatc cttgtcaatg caacgtttgg tccatcagat tcgatattcg
     2821 tcgtcaatgc aggtaccatg atgttttgag ccgtaagcac tttggaacga tcgacttctt
     2881 ccacaaattc acgtacataa tcatttgctg gattggtcaa tatttcttca cctgttccga
     2941 tctgcatgat ttcgccgtct ttcatcaagg cgattctgtc cccaatccgt aaagcttcat
     3001 tcaaatcatg ggtaatgaaa ataatcgttt tttgaacatt ggcttgcagg tccagtaatt
     3061 cgtcttgcat ttctcgccgg atcagcggat caagagccga gaacgcttcg tccatcagca
     3121 agatctcagg atcatttgct aaagctctgg ctaggccaac ccgttgttgc atcccaccag
     3181 ataactgact aggatattgg tctttaaatg aaagcaaact agaattttct aatgcttttt
     3241 ctgctttttc ttgtcgttct tctttaggta caccacggac ttctaacccg tattctgtat
     3301 tttccaagat cgttcgatgt ggaaacagac cgaagttttg gaaaaccata ttgattttat
     3361 gacgacggac ttctcgcaat tcttctttgc ttaacttcga tacatcttct ccatcaatat
     3421 agatactgcc cgaagttggc tcaatcagac gattcaatag acggatcaat gtggatttcc
     3481 cactcccgga aagccccatg atcacgaaga tttctccttc ttttacgtca aaattcacgt
     3541 catacacacc aaccgttgca ccggttttct ttaaaatctc cgttttcggc tggtgatcat
     3601 tcatcatatc taaagcggct tgtgtctttt taccaaatat ctttgttaaa tgttttactt
     3661 ctacttttac caaacaaatt ctcctttttt atgtaaattc atttttcttt ctgaaaaaag
     3721 tgaccacact atgttaacat catgtagtca ctttgtcaaa aatgaaagaa atttgcttgt
     3781 ccaaactgaa aaaaacttgg ctaaatcttt gtaaataaaa ggtttagcca agttaatatt
     3841 tttttaatat ttcacacaat cattctgcaa gctgatgctc cgggtaaaaa aagtttgtca
     3901 tacgctgtag tgtatattca tttccaataa aatgaacgat gtcccctgcc gtaaatttag
     3961 cataggggcc aggtgaaaca agcagttctt cattatgaga aattgcgata actgtggcgc
     4021 cagtcgcctg ccagatattc aactcgctaa ttgaacggtc cagatgcatt gctttttctg
     4081 ataaagataa ttcaaacgga agtaaaggat tcatcttatg tattcgcttc gtttgattga
     4141 ccagctgatc caataattga gaaaaatgat tcagctcatc ttgttgcttc tccacacttg
     4201 ccatgatttc tgcttttgta tcttggatgg attgtacatc ttgatattgc aataggaaat
     4261 cttttgcttt ttccttcgaa gcaacaaatg ccccacttcc atgccgtact tccatgattc
     4321 caagatctac cagcacattg atagccttgc gggtagtctc aggagaagca ttgtacgtac
     4381 tggcaagtgt tgagcgggca tggatcttct cacccacttt atatcggttt tcaacgatac
     4441 gttcggctaa atcaacagca atctgctggt agcgcgggcg gacgattttt ttctttccag
     4501 acattttctt tcctctttct ttttttactt tccctcatct tactatgctc tagcaaaata
     4561 agcaaaaaag gggagaatag cctcttgcaa cgactgatca tcgcttgatc tgttcctttt
     4621 ttactgggca agctgtttga cagtcatcac aattcttacc ggattttaaa cgagtataga
     4681 caattgcgcc tgcacttcca aagattaaca cacttaataa gaatgtcgcc atcctattcc
     4741 tctccttcta atgacaatat agtataaaac tggttcttgg attttggctg acggaacaaa
     4801 acataaacga gtcctatcat cagtaaaata gcaagatacg tccctaacgc tacctgtcca
     4861 ccctccaata agtgtcccaa ctgataaatc acaaaactaa ctaaataagc taaaccgcat
     4921 tgatagccta tggcgatcca agtccatttc ttggcattca tttcccgatg gatagcccca
     4981 attgccgcaa aacaaggcgc acaaagtaaa ttgaaggcta acaatgaata agctgctgcg
     5041 ggagtatatg tgtgttgtag cacttgccag atttctctgc cattttcgga tacttccgca
     5101 tgaccaaaca aaataccaaa tgtgccaata atattttctt tagcgaccaa tcctgtaatc
     5161 gctgcgactg ttccttgcca gtttccccaa ccaagcggct gaaaaacaac tgccaggatt
     5221 cgcccgaaaa aggctaagat actttggtct tcagaaacag gttgagcata aaaattataa
     5281 gaagagctga accagatcac gatactagaa acaaagataa tcgttcctgc ttttttaaca
     5341 aaagacttgc tgcggtcata ggtttgtctt aggaccgctg accattgagg aaaatgatat
     5401 gcgggcaatt ccatgataaa aggagctgta tctccagcaa acaaacgtgt ttttttcaag
     5461 ctgattccag agaaaataat tgcagtcata ccaagaaaat aggcagaagg agctacccaa
     5521 gattgatgag gaaaaaaggc ccctgctact aaggcaataa tcggcaactt tgcagaacaa
     5581 ggcatgaagg ttgtgaccat gatcgtcaaa cgacgatctt gctcgttttc aatcgtgcga
     5641 ctggccatga cgcctggcac tccacatcct gtagcaatca gcataggaat gaaggatttt
     5701 cctgaaagac cgaatttcct gaaaagtcga tccatgacaa aagcgatcct cgccatatat
     5761 ccgcagtctt ccagaaagct caagcataaa aacaatataa tcaattgcgg tagaaatcct
     5821 aacactgctc ccacgcctgc gatgatgccg ttcaagatca gctcctgcat ccatcccgct
     5881 acttgccatt cagctaacaa tctgccaaca tgttctggta accatgtacc aaacaatgtg
     5941 tcattgatcc aatctgtccc cattgttcct actgtttgaa tagccagata atagatcagc
     6001 cacatcacaa atgcgaagat cggcaaggct aaccaacgat tcgttgcaat ctgatcgatt
     6061 ttgtcactca tcgataattg gaaagaatca tttgacacaa cacagagtgt acatagttgg
     6121 gtaataaatt cataacgttc attgaccaga atcgtctcac tatcgtcgtc caatagtttc
     6181 tctgttaact ggatcagttg ctcgatctct ttttgctgag aaactgctat ctcgtaatcc
     6241 tctcttacct tctgatctcg ttcaaaatat ttcattgcat accagcgttt cttgccgctt
     6301 tgttctccta gtacatcttc aatttcatgt aaagctgctt ctaaacgtgg atcatatgta
     6361 cagggaacag gaatagaagg ccgctttttt gcagactcct gaatcttttc gattagtttt
     6421 tcgattcctt gctttttcaa tacgctgatt ggtaaaactg gtacaccaag accataggaa
     6481 agcttttcta gattgatttt ttttccttct tttttgatga catccatcat attcagacca
     6541 atgatcatag gaatgtctaa atccaataac tgcgtagtta aataaagatt tcgttctaaa
     6601 ttggtagcat caataatgtt gatgatcagt tcaggctgtt cctgaaccaa ataatctctt
     6661 gcaatcactt cttctggtgt ataaggagac aaagaataga tgccgggtag gtcctgaata
     6721 tgaatgttcg gatttttttt caacgatcct tctttttttt ctactgttac gcccggccaa
     6781 ttccctacat attgattcga accagtcaag agattgaaca agctagtttt cccgctgttt
     6841 ggatttccga ctaacgcgat attcattctt tttcctccct ttgtatcaag attagttcgg
     6901 cctctgactt tcgtaacgtc agttcatacc ctcgcactgt cagttcgatt ggatctccca
     6961 gaggggctag ttttcggata actattttta catttcgcgt caatcccata tccattaacc
     7021 gtcgcttcac tgcgccttgt ccgtggattg ctacgacgat cccagtctct cctgctttta
     7081 attgatcaag agaagtccag ctttcagagg aagcagtctt tttattgaca aagatttgac
     7141 ctagtatttc ccgacttact gccactcgat gactttttac cagtaaaatg gcattgttgc
     7201 ctgataaatt gacgacgacg attttacttc cggcaacgat cccgatattt tgcaaatggc
     7261 gttttgtttt ttcttctgtt tcgattcgtt cgacttgata aacgaaattc tgctttgcct
     7321 gcgctaaggt ctgcatattg attcctctct tgtgaaataa atcatgtatt aaaatcattc
     7381 attatatgtt ctaattttct tacgtgttac gcaaataaat gagaatcatt ctcacataga
     7441 atactctcgg taattgagaa tgtcaattat acttgtgata ttatgagcga atctttagta
     7501 ttgatagata aaaatgagaa aaaaatattt cttcatagtt ttccacaatt ttttctctat
     7561 taaatttgct attttcccac acgatacttg ttttcagctc ttttttcaaa aacacaaaac
     7621 ttagtattcc acagtttcca caacacaata tgtagtgttt gttgtttgac ttttttacta
     7681 agagttttat actatctatt atagataata agaatgtgtt agaggaggca ctcaccatga
     7741 acgtaaaaat cttttctaaa aataattgta tgcaatgtaa gatggtcaaa cggtttttaa
     7801 cagaaaatca aattgctttt gaagaagtca atatcgatga acagcctgaa gcagtagact
     7861 ggttgaaagc acaaggattc caaagtgttc ctgtcattac ttctgatgca gaaacagtta
     7921 tcggattccg tcctgatcag ttgcgtaaac ttgccagcta acatgactaa aagaatcaaa
     7981 acgtgataca tggatttgaa gggcttgaga cagcaccagt ttttgtcgca aacttccaaa
     8041 tcctttttta aagcttaaaa acaatgcaat gatttgcaat caagcatcat caaatacgaa
     8101 atgaaggttg ggcgtacgta tgagtcttaa aaatttacaa gatgtcagtt atttcaagct
     8161 gaataatgaa atcaatcggc cagtcaatgg tcaaatccct ttgaataagg ataaagaagc
     8221 tttagttgcc ttttttgagg aaaatgtcaa accaaatacg atgacttttc catctattat
     8281 ggaaaaaatc aattacttga tcgatcaaga atatatagaa gcagaattca ttcaattgta
     8341 ttcaccatca tttattgaaa agctatatca atttttggac gagcaacatt tttctttcaa
     8401 atcttttatg gctgcttaca aattttattc tcaatatgcg ttgaaaacca acgacggttc
     8461 cgcttatctg gaaacttatg aagatcgagt agccttcaat gcgctttact ttgcgaacgg
     8521 ggatgaagaa ttagcacttg ctcttgcaga cgaaatgatc catcaacgat atcagccagc
     8581 tacaccttct ttcttgaatg ctggacgtaa acgtcggggc gaattggtgt cttgtttctt
     8641 gactcaagtc actgacgata tgaactctat cgggcgctca atcaactctg ctttgcaatt
     8701 gtcccgtatt ggtggcggcg tagggattac tttatcaaat cttcgtgaag ccggcgctcc
     8761 tatcaaagga tatgagggtg ctgcaagtgg tgtcgttcca gtcatgaaat tattcgaaga
     8821 tagtttcagc tattctaatc aattaggtca gcgtcaagga gctggcgtgg tttatttgaa
     8881 cgttttccat ccagacatta ttgctttcct ttcagcaaaa aaagaaaatg cagatgaaaa
     8941 gattcgcgta aaaactcttt ctcttggcgt gatcgtacct gataaatttt atgaattgac
     9001 acgtaaaaac gaagatatgt atttatttag tccatatagt gtcgaaaaag aatatggtgt
     9061 cccattctct tatgtaaata ttacagctga gtatgatcat ctagttgcaa atccaaacat
     9121 tcgcaaacaa aaaatcaaag cgcgtgatct tgaaaatgaa atctcaaaat tacaacaaga
     9181 atctggctat ccttacgtaa tcaatattga tacagcgaac cgcgagaatc caatcgatgg
     9241 caagatcatc atgagtaatt tatgttcaga gattttacaa gttcaaacgc cttctgtgat
     9301 taacggcaaa caggaatatg aagtattagg aacagatatc tcatgtaact taggctcgac
     9361 aaatatcgtg aatttgatgc aaagccctaa ctttggtcaa tctgtgcgta cgatgactcg
     9421 tgcattgaca tttattacag atgcatctga tatcgatgtc gtaccaccga tccaaaacgg
     9481 aaataagctg aatcatacga ttggattagg tgccatgggc ttacacactt atcttgcaaa
     9541 agaacaaatg gaatatggct cagaggattc attagacttt acaaatattt acttcatgct
     9601 gttgaattat tggacacttg ttgaaagcaa caatatcgct cgtgaaagaa aacaagtttt
     9661 ccataatttt gaaaaatcag cttatgctga cggcacttat ttcgaaaaat acgtaactgg
     9721 agaattccag ccaaaatcag aaaaagtcaa agcattgttt gaaggcattt ttatcccaac
     9781 tgctgaagat tggaatgctt tgaaacaagc agtaatgaaa gatggactgt atcatcaaaa
     9841 tcgcttggca gtagcaccaa atggctcaat ttcatatatc aacgatacga gtgcaagtat
     9901 tcatcctatc actcgcatga tcgaagaacg ccaagagaag aaaatcggta aaatttatta
     9961 cccagcgcct tatctatcca atgagacgat tccgtattac acttctgctt atgatatgga
    10021 catgcgtaaa gtaatcgatg tttatgcaac ggcacaacaa cacgtcgatc aaggcatgag
    10081 tttgacttta ttcatgcgtt ctgaaatccc agaaggcatt tatgaatgga agacaacaac
    10141 gaagcaaacg acacgtgatt tgaatatttt aagacattac gctttccaca aaggaatcaa
    10201 atccatttat tacgtacgta cctttactga tgatgcagaa gaaatcggaa gcaatcaatg
    10261 tgaaagctgc gtgatctaat atcatagata gattcgctta ttcatatttt aattaaagga
    10321 gcattctcat gtcagaaaca tattatgaag caataaactg gaatgaaatt gaagatatca
    10381 ttgataaatc cacttgggag aagctgacag agcaattttg gctagataca cgtatcccac
    10441 tttcaaatga cctagatgat tggcgtacat tgtctcaatt ggaaaaagat acagtaggac
    10501 atgtatttgg cggtttgaca ctgctagata ctgtccaatc tgaaagtggc atggatcagt
    10561 tgcgtaaaga tgtccgcact ccccatgaag aagccgtttt gaacaatatt caattcatgg
    10621 aatccgttca tgccaaaagt tattcttcta tttttagtac actgaatacg aaaaaagaaa
    10681 tcgaagaaat ttttgactgg acaaatacga atccttatct gcaaaaaaaa gctgagcgta
    10741 tcaatgaaat ctacaaacac ggtacaccgc tagaaaagaa aattgccagc gtctttttag
    10801 aaacattctt gttctattca ggtttttata ctcctcttta ttatttagga aacaacaaat
    10861 tggctaacgt agcagagatc atcaaactaa tcatccgaga tgaatctgta catggaacct
    10921 acatcggtta caaattccaa ttaggattca acgagttacc tgaagcagaa caagaaacgt
    10981 taaaagactg gatgtacaat cttctttatg agctttacga aaacgaagaa cgttatacag
    11041 aagaattata cgatccaatc ggctggacag aagaagtcaa gacattccta cgctataatg
    11101 ccaacaaagc attgatgaac ctagggatgg atccactctt tccagatact gccaatgatg
    11161 tcaacccaat cgtaatgaac gggatttcaa caggcacaag caaccatgac ttcttctcac
    11221 aagtcggaaa cggctatttg ttaggtcatg tagaagcgat gaaagacgac gattatttga
    11281 ttggattgga ataaggattt attttaattc aaaaattttt cgaaaaataa aaagagttta
    11341 gcgtacagat attttaaatg tacactgaac tcttttttat gcttattcac ttgtttcttc
    11401 cagcactttt ggactgtcta acttttccga aacatgttcc attcttctca ataaatagat
    11461 tttccggata tttaatatta tcgtcttaat cactgcatag acaggtatgc aaatcagcat
    11521 gcctaatatt cctgcgacac ttcctgctcc aatcaatacc aagatgatcg tcaaaggatg
    11581 gatattcatt gatttcccaa ataacaacgg cttgatcaaa tttccatcta attgttggat
    11641 cactaaaatc gacaacgcca tatacaaagc ttgaaatgga gagataaaca aaccgacaat
    11701 gattgctggt accgctccaa taaaagggcc gacatacggg ataatattcg tgattccgca
    11761 aatgaccgca agcaacaagc tataaggctg atcgaatatt ctcattgcca aaaagctcat
    11821 tacaccaata atcaaagcat ctaagatcgt actgctgata taggcagata atgtttcgtt
    11881 taattctttt accgtttggc gtaactcttt acgaatcgct ttcggaaaga agtgagacaa
    11941 cgcatcaata aatttgtgcc catccttgaa cataaatagc aaaataaatg gaacggtaaa
    12001 taacaaaata aaaaattgca tcataaatga aatgattttt gagacacttg cagataaact
    12061 gactaatatg atattgaaca aattactaag agataaattt gctgatgcta attgttcttc
    12121 caaattgaaa tttttaaact cttcccgctg cgccaattca tttaaccatt tgcttgtttc
    12181 atctgcatac attggcaagc tctgggtcaa ttgaattgtc tgttccatta actgaggcac
    12241 tacattcatg acagttaaaa caactaaaat gattacgacc aaaaacgtca ataaaaaacc
    12301 gaacactctc gggatccgat gcttttcgag aaaaacaacg attggatcga acatataata
    12361 gataaaacca gcaacaagca atggtaaaaa aattgccgaa agcattttca atattggact
    12421 aaaaatattc ggcatttgta aaataaagta cacgccgatg atcgtgagta ctagctccac
    12481 tgtccagaaa aataattttg attgtttaaa acgtgattgc atacatctcc tccaaggtcc
    12541 tttgatactt ctattctatc atttaacaaa gagttacgcg ctgagatttt catttgctta
    12601 aaaaaaacaa aaaaaataaa catattttaa tgagtagacg agtacttaaa caaaaacacg
    12661 gtttggagta gtgacaaaaa ttatactgca gtcccttttt ccaaataaac ggtgttcaaa
    12721 agcttgcatc ttcgtaataa gcccagccaa acgaaaaata taaggaacta tttttatttg
    12781 gctgggctta ttacttgatg taggacatct attttacaac ctcagatcat ttattgcgca
    12841 atactgacta aatgcaactt tgtgatattt ttgtgtacgg acaactcctc gatcacatcg
    12901 gcataagtga gcccttttgg aagatcaatc gtatagatat ttttgtacac tcggtagtca
    12961 tcaacgaatt ttacgtcaaa attcacatct tcgattacga tatttttctc ttcaaaataa
    13021 tcggtaagaa aatctttggt ttcttttcta tggataaatt ggatttctaa ctttttagtc
    13081 gtaggtacat ggatcacgcg tttcactact gtgagtgcaa tagcaatccc gataaaactg
    13141 atgatcgcaa tggaatagta acccatgccg atggcgatcc ctaatccagc aattgcccat
    13201 aaagaagcgg cagtcgtaag tcccgtcacc gattgtttcg tgacgatgat cgttcccgcg
    13261 cctaagaaac cgacaccact caccacttga gcaatcagcc ttgcttcatc tgaacgaatc
    13321 gttccagcaa gctgtggata ctctaaagca tctcttaatg caccactagc gatctcaact
    13381 tggatcaatg caatgataca tgctcccata caaactaaaa tatgcgtacg catacctgct
    13441 ggacgatttt tatattgtcg ttcaaaaccg attgctccgc caaatagcat agcgatccct
    13501 aaacgtaaaa taatctctgg aattgtcaat accatatcat tcatctaaac accctccagt
    13561 ccaatatatc atgtctgaaa ggagcagaca aaaaaagtgc aatttttacc tcttacgaaa
    13621 aaaggactgt gacaaaattt ttgtcatagt cctttttatg aataaacggt gttcaatcag
    13681 ctgaccctcg gtattactca acaaacggca aaatatgggg agctattttt cctttgtctt
    13741 ctctaatact caggtcaaaa cgctgatttc acaacctctt tagcctacta ttgaaaatta
    13801 caacttactt gctgcttctt catcgatgat cacaacaaca ttgtcgtgat tttgcaatac
    13861 acttgctggc aaatgattcg ttacaggtcc ttcaatcatc cctttgattg catcagcttt
    13921 gctttcgcca aatgccatca agacgatttc tttacttttc ataattgaac cgattcccat
    13981 tgaaacagct tttgttggta catcttctgc tttatcgaag aatcgtttgt ttgcattgat
    14041 tgttgattct gttaagtcaa ctactgaagt cgttccgtca aatgaagcac caggttcatt
    14101 gaaaccgata tgtccatttt gcccgattcc taagatttgg atatcgattg gatgtgcatc
    14161 gatgatttga tcatactttt tgcatgctgc ttccagatct tctgcttttc catctggtac
    14221 aaacgtttct ttgaatggtt ttttatcaaa taattggaca tccatgaaat gacggtagct
    14281 ttgttcatct gaaccgccta gtccaacgta ctcatctaag tttacagaag tcatttcact
    14341 gaaatccaaa tcgcttgaag tcatttcttt atataaagta actggtgtgc ttcctgttgc
    14401 caatcctaat acttttgcac ctttgttcat accttctttg atgatttcaa atgctttttt
    14461 tccgccttct tcagcgtttt ttacacgaat gatttccata gtgattgctc ctttatattt
    14521 tggtatagcc caattatagc attctttttt tctttttaca ataattagta tagaccaaat
    14581 tgcatttttt tcattttctt gacaataaat aggaatccgt gttaggtttc ataagatggt
    14641 tcaatcaaac aatccatgtt gcatgaaaac caagcaagtg attcactgtg gacgctttat
    14701 gtctgcagtt tttttatgaa aaaggagtta ttatgagtat actgacatta aacaacatta
    14761 cccagcaatt tggcgacaaa atcctctatg aaggtgtcga tctgcaatta aatgctggcg
    14821 aacatcttgg gctgattgga caaaacggag ccggtaaaag tacattgatt aaaatcatca
    14881 ctggtgagat cttaccagat gacggacaga tccattggca aaaaaatatt cacaaaggtt
    14941 acctcgatca atatgtggaa gtcgatgaaa cattaacaat cgaagaattt ctaaaaactg
    15001 cgtacgcaaa agaatacgaa aaggaagaaa aaattgcttt tctttaccaa acatatagcg
    15061 agaccttcga tgattccttg ctggaaaaag ctgggaaact ccaaacggaa ttagaccaag
    15121 gagtgtttta tcaaatcgac accttagttg ctgagatgag cagcgggttg gggatcgatg
    15181 tcttaggttt agattcactg ttgaaaaatc taagtggtgg acaacgttca aaggtgattt
    15241 tagcaaaact acttttagaa aacccagatg ttctgatctt agatgaacca accaatcact
    15301 tagatgatca gcatatccaa tggctgacgc agtttcttca ggattttgac ggtgcgtata
    15361 tcgtgatttc ccatgaccaa gaatttcttg atcagatcac cacccatatc gcagacatcg
    15421 agtttggaaa gattactaag tatactggcc atctaaaaca agcattgaaa caaaaggaac
    15481 aaaatcgcga aagctatttg cgccaatatc atgctcaaca aaaacaaatc gaaaagaccg
    15541 aagcttatat tcgaaaatac aaagccggtt cccgatcaac gatggcaaaa agccgacaaa
    15601 aacaattaga taaaatcgaa cggctgacgc ctccagcttc agcaagtaaa gctgtctttg
    15661 attttccatt ctcgcctatt gtcacgacat tagcagtgga aacaactgat ttagtcatcg
    15721 gatatgatcg accgctactt gcacccatca atttgaccat ccgctttggt gaaaatattg
    15781 ccattcgagg cttcaacggg attggaaaat ctactttatt aaaaacactg atcggtgaga
    15841 ttgaaaaaat aagtggcgat ttccacttcc cagataacac aaaaatcaat tacttttcac
    15901 aagatctgta ctgggaaaat ccattagaaa caccactaca atatttaagc agtcaatttc
    15961 caaaagcgac tatcaaagaa ttacgtcgcc aactagctaa agcagggtta gttaatcaat
    16021 tagcaagtga acctctcact acattaagtg gtggggaaca aactaaggtg aaacttgcac
    16081 agttgacaat gaaccaaggt aatttactga ttctcgatga accgacgaac catatcgatc
    16141 aagaaacgaa agaaagctta caagacggga tacagaaatt tccaggaaca gtaatcatcg
    16201 tctctcatga acaagaattt tatcaagatc ttgtgcaacg agtcattgag attgaaccaa
    16261 aataaacgtt tgattgaata gttgaaaaaa aggagctgtg acaaaagttt tgtcacaact
    16321 ccttcttctg aataaatggt ggccaattag ctgaccttcg gtattagctc aaaaaacggc
    16381 aaaatatgag gagctatttt tatttggcta ttcttattac ttgatgcaga acagcttttc
    16441 acaacctctt ttcgtctatt ttttattttc gagatttctc tttcctttaa aaacaactgt
    16501 ttgaaagaag aacgcacatc cttgaaaaac aagcccgcta accgttacac ctaaccagcc
    16561 atactgctgc cacattgtcg tccctagcaa tgaaccagcc gcacctccaa taaagtacat
    16621 aaacataaat aca
//
LOCUS       DACDPS010000052        16389 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.052, whole genome shotgun sequence.
ACCESSION   DACDPS010000052 DACDPS010000000
VERSION     DACDPS010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.052"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..525
                     /gene="scm"
                     /locus_tag="IWJ07_001841"
     CDS             <1..525
                     /gene="scm"
                     /locus_tag="IWJ07_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP9333419.1"
                     /translation="TSASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSD
                     STDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSD
                     TRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVI
                     GLRITFMSYKKSKR"
     gene            complement(680..1666)
                     /locus_tag="IWJ07_001842"
     CDS             complement(680..1666)
                     /locus_tag="IWJ07_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP9333420.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(1689..2255)
                     /locus_tag="IWJ07_001843"
     CDS             complement(1689..2255)
                     /locus_tag="IWJ07_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAP9333421.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(2269..2685)
                     /locus_tag="IWJ07_001844"
     CDS             complement(2269..2685)
                     /locus_tag="IWJ07_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAP9333422.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(3142..5076)
                     /locus_tag="IWJ07_001845"
     CDS             complement(3142..5076)
                     /locus_tag="IWJ07_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9333423.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5378..6313
                     /locus_tag="IWJ07_001846"
     CDS             5378..6313
                     /locus_tag="IWJ07_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAP9333424.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            6403..7506
                     /locus_tag="IWJ07_001847"
     CDS             6403..7506
                     /locus_tag="IWJ07_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP9333425.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            7519..8472
                     /gene="menA"
                     /locus_tag="IWJ07_001848"
     CDS             7519..8472
                     /gene="menA"
                     /locus_tag="IWJ07_001848"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAP9333426.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(9407..10753)
                     /gene="gor"
                     /locus_tag="IWJ07_001849"
     CDS             complement(9407..10753)
                     /gene="gor"
                     /locus_tag="IWJ07_001849"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAP9333427.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(10887..12128)
                     /locus_tag="IWJ07_001850"
     CDS             complement(10887..12128)
                     /locus_tag="IWJ07_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP9333428.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(12146..13069)
                     /locus_tag="IWJ07_001851"
     CDS             complement(12146..13069)
                     /locus_tag="IWJ07_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP9333429.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(13290..15782)
                     /locus_tag="IWJ07_001852"
     CDS             complement(13290..15782)
                     /locus_tag="IWJ07_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP9333430.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(15789..16256)
                     /locus_tag="IWJ07_001853"
     CDS             complement(15789..16256)
                     /locus_tag="IWJ07_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAP9333431.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         4820 a         3424 c         2918 g         5227 t
ORIGIN      
        1 acatcagctt caagtgacag tacagatact acctctacca caagtgatag cacggacacg
       61 tcagcttcaa gcgacagtac ggatacatct accacaacaa gtgatagcac ggacacgtca
      121 gcttcaagtg acagtacaga tactacctct accacaagtg atagctcaga ttcatctacc
      181 actacaagtg atagcacgga cacgtcagct tcaagtgaca gtacagatac atcctctacc
      241 acaagtgata gctcagattc atcaacttca agcgatagta cagacacgtc gtctaccaca
      301 agcgatacac gtcatgcaag tgtctccttc tcaaaaacaa gttcggataa caggacgaac
      361 tttaacttaa ctaataaaac aagcaatgac aatggaaaca aatcaaaaag agcacttcct
      421 aaaactggaa gtcaatcgaa taattggata acactcgcag gcgttatatt acttgtaatc
      481 ggtttaagaa tcacttttat gagttataag aaatcgaaaa ggtaaaaaaa tattcaagat
      541 aaaaaagagc aacgtcgcta acggacgttg ctctttattt ttgcttttta tcataaatac
      601 aagaactaca attcaatttg tcaaaaaaca cgtagatttc cttctgagaa atttatttac
      661 gtatcgaacc tttccctatt cagtctttgc gatccaaaat cagttgggta agggaaaaga
      721 gattctcttt tgttcgaaga gaggtggcag gtaatttctt tatttccttc aaagcctttt
      781 ctgtgtaaga ggccgctaac ttcctagctt cttccacgcc tcctgtatgt tcaacgattt
      841 tgcgtacggc gtcgacatct tcttctgtca ttgccgcttt cttctctaaa taaggtagta
      901 gctttggttt gtttttttgc aaagagtaga tcaacggtaa agaatagacc ccttgacgca
      961 tgtcttctag tacaggtttg ccgatttcct ggctctcttt tgtataatct aaaatatcat
     1021 cgataatctg aaaggccatc ccgatagaaa gtccgatatc tcctgctttt tttgcgaagc
     1081 gctcgcttgt tccactttca aaagcgccaa cagaacaact taattggaac agttcagctg
     1141 tcttacctga aatattttcc aaatattctt ttacagaaag ttcataatta tagcgatcat
     1201 ccatctgccc taattcgcca ttcaatactt tttccatact gcgagaattc aattggagac
     1261 ttttcaagga agtggagtaa tcagacaata gtttaaaaca gcagacaaat aaatagtctc
     1321 ctgcatagac agaaacgaca ttaccaaact gactacggat attaggctga ccacgtcgca
     1381 gatccgcttc atcaacgata tcatcatgga tcagtgtggc cgtatggagc atttcgatag
     1441 aagctgccaa agcgatggct ttgtctcgat cttgctctgg tccaaattgt gagaacaata
     1501 gctgataggc aggacgcaac agctttccgc cggcatggat cgtttctttg attgcttgtt
     1561 cgacaggttt gtttttcagg cggacagttt cttccatccg ttttagtgtt tctgtcaatt
     1621 ctttacttaa agcaggatag ttttcccaca ttggatgtag cttcataaaa aaactccttg
     1681 tgtgacgatt attttttggt aaacattggg ctccattttt gtttggttcc ctgctcataa
     1741 tctttttgga atcgttgaag tgtctgcaca tgtcgtaaaa gataattcgc tgcgatcccg
     1801 attgcaatcc ctgataaaat ccctaaaact gaaaggactg gcaagtacaa catgactgtc
     1861 catgattgtg caatgaaaga agccatcaaa agctgtccga cattatgcat gaaaccgccg
     1921 gccgcactaa ttccgatgat actgacacgt tttggtccta attgcttgac caataacatt
     1981 ccaaaataac taagcaaagc accactcata ctataaagga atgtggaaat cgttccgcct
     2041 agcaaagtag tcaggaatag tcgtagccaa agtagtaaga agctgtctcg ttttttcatg
     2101 gtaaaaatgg caacgatcgt gattagattg gctaagccta gttttgcacc aggcgcaaaa
     2161 gaaaaaggat aagggatcat attttccaat aaaccaatga tcactccttg tgcaacaagt
     2221 agcgaaatat aaatcatttt ttgtaattta ctcatggtgt gtgaaggtct aataaatcag
     2281 actgtcttcg tcacctccat ctgttgaacg gacttcgatc actaatttat gtggcaaaca
     2341 gacgatggtt tcgccatttt tcgttgccca gcctcgacgt acgcagatct ggtcatcgca
     2401 atctgcttct tttatccgta ctcgatcgcc atctatctcg atcaagttat aatctccgtg
     2461 ttcatctttg taagtatatg tgttgctctc ttctccagcc atcaaaggga atgttttgat
     2521 ttctgtgcca tccacacgca gtaccgcttc tttttctgct gttgcggtgt tttgctgaag
     2581 accaaaaatc atgactggta aaaaagaaag aacagtcaaa agcaaaataa taaaaacatc
     2641 ccaaggtttg agcagacttt tttcagcaaa ttctttaagg ctcatttttg ttctcctttt
     2701 tccagattaa ctcacccaat tttagcataa aaacaagagg ttgtgacacc tgtgtcttgc
     2761 tttgagtaat aagaacgaag aatagaaaac agctttccat gttttctaat tctttgggct
     2821 tattaccgag aagcagcagg tggaacaccg tttataacaa gaggttgtga cagaagtggg
     2881 caacttcaag aaataagaag gaatttacga aaattacttt tcaagttttt gtgaaatttc
     2941 ggcttatttt tcgaagaagc tacttctgga acaccgttta taacaagagg ttgtaacaga
     3001 ggttgtgaca cctgtgtctt gctcatggag gcttatctcg ggcatcttat tcctttaatt
     3061 ttacataaaa aggaggctgt gacggtttgt ctcagcccct ttctattgaa atcttgcatt
     3121 caaataacgt tctttatcta tttatttctt gccgtactgg gaacttggtc taccgtacca
     3181 ccaattacct agtgtacgtt gtagccatgg aattacccag cgatccaacc caatcgcacg
     3241 tccggaaccg ttcatcaatg cgaaagcaac aaaaatgaac caaatgttta cccagtaaaa
     3301 catgcctgat aagcagaacg tgatcaccaa tacaatcgtt gtcgcactgc atagccatgt
     3361 gaataatcca gcaatcaatg ctaatgccaa ggcaatttca aagaatacca tgaatttttg
     3421 gaagaacatt gctacatcat tgtttggcat gatcacttgc atgatctttg tgaaccagtc
     3481 tggtgacttc tcaatcacag ccattggttc ttcaccgtat gcataactca aaccaaaatg
     3541 tgcagcttga gtcgctgctt ctccggcact tgcccctgct ccgctggcag ctgtggttgc
     3601 atcaccagca gcagaagcac cactggcaac atctgttaca ttgtattccc atgtccaagg
     3661 gaacacactg tttccagtga accatgatcc ttcaccaaac caagtacttg gtttgaacac
     3721 ttcaccattc ccaatgattt ttttggaagc ttcaacgaac caaactaaac catagaaaat
     3781 acgaagtggc acactccata agacattgcc gtaacgagaa gaatgtcctc gagtcacgtt
     3841 gcgatcatct ttgatatgga aaaactcatg cattaaatat tggaacatat aataaccaga
     3901 acgaatatca aagaagtatt tcaaattcac gatatgtttc attacgatag ctaaaaatcc
     3961 gcttaaatgg atcttatcaa ataggttagc taccccccat ttggcaccaa tagaaaccat
     4021 aaatccttga tagtttcctt taaatttatg cttttctccg cctttgatcg aagccacgat
     4081 attcgctgcg gctgtgtgtc ctgtttgttc agcagcttga acgatctgcg gtgtcggtgt
     4141 tcctgggaat tcttcataaa agactaaatc accaatgata tagatgtttt tatcttcgta
     4201 gccttttgcc tgcatatatt cattggcgat caaccgctgt ccgcgtgctg attctaatcc
     4261 aaagtcagca gcgtcagaag ttcctttgac accagctgtc caaatcagtg tgtgagtcgg
     4321 aatgttgcgt ccgtctttca atttgatatg atcagcagcc acttctacga ttggtgcatt
     4381 caataataat tccacgttgt gcttacgcaa atagcgttca gcttttgcag catcatttcg
     4441 atccaacata ttcaagatcg tcggcattgc ctctacgacc atcagtgtga tttcgtttgg
     4501 atctaattta taatctttgg ctaggcgatt tttccagtcg atcagttccc caatcatttc
     4561 aataccggta aatccagaac cacagacaac gaaagtcagc atggctttac gcagttcatc
     4621 atctggttcc aatgcagctt ttgctactgt cttctcaata tgttcccgaa tccttacaga
     4681 atcttcaaat gaccaaaggg tgaatccgtg tgctttgaca ccaggtgtac caaagtcatt
     4741 cggctctccg cccattccaa ggatcagctg atcaaaagga taggaaccta atttggtctt
     4801 gacgattttt cgttctttat cgataccgat tacagtgtct gtgacaagtt gtacattctt
     4861 ttgacgagca aacaaatgct gcagatcgta ttggattgct tctggttcta cacgtccgcc
     4921 agctacttca tgcaactcgg tcatcatagt atggtacgaa tgacgatcga tcagagtaat
     4981 tgctacatct tcttttttca gtttctttgc caagtatttt gtagctgaaa caccggcata
     5041 tcctgctcct acaacgacaa tattttgctt tgccattgat catccgctcc ttatagacta
     5101 tctatataat gaatatatga taattctttt agtttgatac ttattacaca atctccatat
     5161 attatataga tgttctgttc ttttgtctaa ctccttttgg atttttttca aattttaaaa
     5221 taaagcgttt tttcacgaaa tttgctgaaa gaaaacgttt gaaatattac atgggttatt
     5281 tatcaaaaat agttgactta taggctaaaa agattagaat gtataatgaa tcacaaagaa
     5341 aaattgtgga ctagaaatgg aatggtgggg tattaaaatg aagaaaaaac gttttgtaac
     5401 tggatttgcc gtattagcat tttcagctct agtgttaggc gcttgcggcg cagatgacaa
     5461 cggaagctca aactcttctt ctgaatcatc tacagcacaa tcttcgactg caaaaagcac
     5521 aacagaatca tctgcaaaag ttgtagctgg cggagatttg caagacggca catataaatt
     5581 agaagaaaaa aattattcaa atggttaccg tgcagtcttt gaaatggttg taaaagacgg
     5641 caaaatcact gaatcaaaat atgataacgt caatgaaaac ggcgaatcaa aaacaaaaga
     5701 tgcagaatac aacaaaaaca tggaagcaaa atctggcaca aacccagaaa aatacattcc
     5761 tgagttgaat gaccaattct taaaagcaca aagcgcaagc ggtgtggaag tcgtaactgg
     5821 agctactcac tcatctgaaa gcttccaaaa ctacgcacaa caattgatcc aagctgctca
     5881 agctggtaac acagatacaa tcgaaatcga caatggtgct gatttgaaag acggcactta
     5941 caagcttgaa gaaaagaact actctaacgg ctaccgtgtg caattcgaaa tgacagttgc
     6001 tggcggcaaa gtaactgaat caaactttga ttatatcgac aaagacggta aatcaaaaca
     6061 agatgataca gaatataatg aaaatatgaa agcaaaaagc ggtacagaac caaaaactta
     6121 catccctact ctgaacgacg aattcgtcaa agcaatgggc gaagaagatg gctcacctgc
     6181 taatgtagaa gttgttacag gtgctacaca cagctcacat tcattcatca tgtacgcaca
     6241 acaattagtg aatgcagctg aaaaaggcga cactcaaaca atcgaagtag ataatatcgt
     6301 aacagaaaaa taatcacata atcatactaa taaaagttgg agggtgggac gatagccaga
     6361 ttaggcagtc gtctcacctt tcaaaatgaa gagaggaaaa agatgcggaa aaaactacta
     6421 ttttccttaa tcacactggg catctctttt atggtgctaa ctggctgcag caactcagca
     6481 aataaaacaa ccactgaaag ttcagcagct accaagatat tgaaagatcc atattctgat
     6541 gaacagttct tgttagggac ttacgttcgt atccgtgtgt atgatgaagg caaagaatca
     6601 gcattgaaac ctgccttcga tcgagtaaaa gaacttggtg ataaaatcac gatcaatcaa
     6661 aaaggttctg agatcgatga agtgaatgag caagctggga tcaaaccagt aaaagtttct
     6721 gatgacgtct acacgttagt caagcgggcc tacgaatata gccaagattc tcaaggcgga
     6781 ttcgatatgg cgattggtgc aatcacacaa ttatggcgaa tcggttttga tgacgccaga
     6841 aaaccttccc aagaagaaat cgatcaggca ttgaaattag tggattatca taagatcgaa
     6901 ctaaatgaca aagaaaaaac tgtttatcta aaagaaaaag gaatgatcat cgatttaggt
     6961 gcaatcgcca aaggttacat tacggacgaa gtagtgaaag tcttgaaaaa acaaggagtg
     7021 accactgcca tcgttgactt gggcggtaat gtctatgtct taggccacag cccaagaggc
     7081 gaaaaccaag actggacagt cggcatccaa gatccgaaca tggctcgcgg atctgtttta
     7141 ggttctatta aagaacggaa caaaacatta gttacctctg gaatttacga gcgttattta
     7201 gaagtagatg gcaaaaaata ccatcatttg tttgatccaa aaacgggtta tccgttcgac
     7261 aatgatatcg caagtgtcac gatcataaca gataaatcaa ttgatggtga cgggttgtct
     7321 acagcagtct tttcaatggg tgtgaaaaaa ggactggaat acgtcgaaag cgaattgaat
     7381 aacgggacgg aagcgatctt cgtgacaaaa gacgataaag tatatgtgac cgatccaatc
     7441 aaagacacct tcaaattatc ggaggactcc cattacacga tgggtgaccg ttctgaacta
     7501 aaataaggag tacgaaccat gacactaaaa atctttttag aagtagttga aatacgaaca
     7561 aaggtggcta gtgtatttcc ttttgcggta ggggtattat tctctatcgc tttttttcat
     7621 gagatacatt ggctaaacac ttttttattt tttattggaa tgctagtttt tgatttagcg
     7681 actaccgcta tcaataatta catggattat gaaaaggctc attctgaagt atataagtat
     7741 gaagaaaatg tcattggccg cagaaatcta tctcctgtat tgattcgaaa catgatttta
     7801 gctatgattg catttgtttt tgttatcggc gttattctaa cgatcaatac gggttggctt
     7861 ttacttttga tgggatttat ctgttgtttt attggagtat tctatacatt cggaccgatt
     7921 cctttatctc gaatgccttt aggggaaatt ttcagtgggg tgactatggg ccttggaatt
     7981 tttgccatgg tcgtttatgt caatacattc ggttcagatg tctatgatct gacagtcaac
     8041 ttttcatctg gacaatttgc tttggtagga aatctttggg ctatagcagc aatcgtatta
     8101 gcttccttac ccttggtgtt tacgattgcc aatatcatgt tggctaataa tttgcgggat
     8161 cttgaccgtg atattgaaaa tcatcgctat acactagttt tttatatcgg acgtcctgcg
     8221 ggaacagtct tattccagct attgatgtat gcttgctatt tcgtgatttt aatggggttg
     8281 ttgagcggta tctatgaatg gccgatcttg gtcaccttct tgaccttgcc aattgtgaag
     8341 aagcatttgg atcaattcaa gcaaacgtta ccccagccta tcagtttcag ctattccatc
     8401 aaaaatatgg tcttattcaa tgccagttat gtattggggc tattactaac aatcattatg
     8461 caagccattt aacagcatga agaggttgtg aaaaaactgc cctgcatcaa gtaataagaa
     8521 cagccaaata aaaatagctc cttatatttt tcgtttggtt gggcttatta ccgcagatgc
     8581 aagcttttga acaccgttta tcaggaggtt gtgaaaaagt cgcttagttt cgagtattaa
     8641 gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa atttggctta ataccgagaa
     8701 actggctttt gaacaccgtt tatcagaggt tgtgaaaaag tcgcttagtt tcgagtatta
     8761 agaaaaattt tgtgaaaatg gctttccaca tttttgcata aatttggctt aataccgaga
     8821 aactggcttt tgaacaccgt ttatcagagg ttgtaaaatc agtgttttgt cctgagtatt
     8881 agagaaacaa atggaaaaat agttcctcat attttgccgt tttttgagct aataccgaag
     8941 gacagctgat tgaacactat ttgttcggaa aaagtgacta actatgacac aatttttttc
     9001 acagtccctt ttttcacttg atttgattcg taccagcgaa cttgacgaga actttcttgg
     9061 tttggtaact atatggtatt cttttaacat cctcgtttgc ttcttctaat ttatttatac
     9121 aaagtcacac ttttttaaac atctgttctt cgatacattt ctatttcttt taaacatcga
     9181 taccaatatt ttgaacttaa gagtgcatac caaatggatt catttactct tcgtcagtta
     9241 cgaagataat caaaatctcc aacttcccta cttatttcgt tttgagaatt atcgaagcaa
     9301 tcttacataa ataactaaaa aacaaaaaac cagaaaatca gtatactgat tttctggttt
     9361 tacatggttt gttcaataaa aagctgttgg aactgttaat attaaattat cgcatcgtta
     9421 cgaattcttc cgctcctgtt ggatggatgg ctactgtatt gtcaaagtct tctttagttg
     9481 cacccatctt gatagccacg gcaaaacctt gcagcatctc atcgacacca acaccaatag
     9541 catgtaagcc gacgattctt tcttcttttc cgacacagat caatttcatt tcgcattttt
     9601 ggcgataatc atttaatgca aagtacattg gggtaaagcg tgaacgataa actttcaatt
     9661 cattttcgcc atatttttcc aatgcttctt cttctgtcaa accaattgtt gcaattggtg
     9721 gatgcgtaaa gacgactgtc ggaacaagtt tatagtcaag ataagaatct ttctttccat
     9781 taaataaacg ttctgataaa cgtctaccag ctgcgattgc aacaggagtc agatctaatt
     9841 taccgatcac atcaccaaca gcaaaaatat gttcatccgt tgtattttgg tatttatcta
     9901 ctttgataaa cccttttttg tccaattcca catttgtatt ttccagacct aatttttcgg
     9961 tattcggtac acgtccaccg gcaaaaagta cacaatcacc agttaaggtt tcaccatttt
    10021 caaatagcac cgtatattcg tcattgtttt tagtaatttc tttagcaata tgatgtgcgt
    10081 aagtttttat tccagcttct tggtacattt ggatcaaatt gtccgaaagc attttatcaa
    10141 acgttctaag tggtcgttct tttcgatatg cccacatgac ttcactacct aaaccacgaa
    10201 atacacctga aagttcagcc gcaatgtatc cagcacctaa aacgattgct ctcttaggta
    10261 actctgttaa ttcaaaaaat ccatttgaat caattgcgta ttctccacca ggaatggtca
    10321 ttttacttgc acggcctcca gtagcgatca agattttagg agcacgatag gtttcaccgt
    10381 ttacttcgat cgtttgacta tcgataaaag tagcgtaacc ttttatagcc tcgacatgat
    10441 tgctatctaa tcctctttga taagcaccgt gtagaaaatc aatatatttt tctcgtcttt
    10501 ctactagacc ggcgaaatca acatttttta aatctgcttg gataccgtaa cttgaggcat
    10561 ctcgttcaat tgtttccaaa atcgtacttg cttgccacat aactttttta ggtacacaac
    10621 cgacgttgac acatgttccg cccaattcat tcgcttcaat cagcaaaact tttgcgccat
    10681 gcatccctgc tctgtttgct gaagcaatac cgccagagcc gccaccaatt acgatgtaat
    10741 catatatttg cattttgttc ctccttattg aagagatcaa tcaaaacctt tatttaacga
    10801 tcctttttct acagaaaaca agtgtaacac ttttcttgaa ttttgtatgc gaagatgctt
    10861 aaaacaggga tatatcagct ttttccttat ttacgttttc ggatcatatc tccaggtttc
    10921 accggctgtg cgactggcat agttaaaatc atcattggat ttggtgctcg gtcaatggat
    10981 tcgccttctt cattatgcat aacttcaacg gtttgactga aatgactgaa tcctggacca
    11041 taaaattcga tttcatctcc tacagaaaac aagtttctct gacgtatcgt agcaatctgt
    11101 gttgcctcat cataagataa tacttcaccg acaaatttat atactgggat tttacgacgt
    11161 tcaccgaata attgttcgtt ttcagttggt gtattgtagt aaaaacctgt agctaattct
    11221 cgttgtgcta ctttccataa ctcgtcgatc cattcttgct tgcagacata gttttcagga
    11281 tcttccatat aactgtctac agccgcttta tagacatttg acaccgtaga tacataatgg
    11341 atcgatttca ttcttccttc gatcttgaag ctgtctacgc cgttttcaat caattctgga
    11401 atatgttcga tcattgacat atccactgca ctcatcgaga attcttcttc cacttcgcct
    11461 gtatcagtta aactgtttcg ttcttgacca aaaggcatat cgaacaatcc atacttccag
    11521 cgacaagatt gagaacatcc gccacggttt gcatcccgca tcgacatatg gttggacaat
    11581 gtgcatcgac cagaataaga gatacacatg gcaccatgga taaatgcttc gatttcggta
    11641 tccgtatttt tacgaatctc tgccacttcc tccattgaaa cttcacgagc taaaacgaca
    11701 cgttccaacc cttcttcttt ccagaattcc aatgtctcaa aattagttgc tgaagcttga
    11761 gtggataaat ggatcggcag acctggggct tctgtcgcac aaatttcgat caaagctgga
    11821 tcagaaacaa tcactgcaga aataccgaca tcacgcaaac tgcggaaaaa ttcccctgct
    11881 ccatcttcat ttccttcatg ggtgaccata ttagcagcaa cgtatacctt cgcatcatgt
    11941 tcttttgcaa aagcaactcc ttcagccatt tcttcataag taaaattacc tgcacggctt
    12001 cttaaaccat atgcattccc gccaatatat actgcatctg ctccataatg gatcgcggtt
    12061 tttaactttt ccagtgttcc tgctggcgct aggatttctg gtcgtttcaa ttttcttttt
    12121 actgtcattt ctttcatcat actccttatt tgatagcaga aggatcaata taaaagaatc
    12181 ctgtatctaa ttcacggttt tcaggatgca atttactgat ttcgctagtc attttttccg
    12241 ctttttcttg atcccagcta ccttctaaaa tttgtttttt tgcttcggcg aatagtcgag
    12301 tgatgtttac gaatttttcg cctggagtaa ataatccgtc caatttccat gtacagtaat
    12361 gatgctgata aagttcattc aattcattcg agagattcag atcattgctg gcaaaaatat
    12421 gtgtgccatg actatcttca taaatagaat aatgtgtctc ttctttttta ggttccgaga
    12481 taaaaagacc tctttcacga tctttttgtt cgtcttgttt cgtatagtta tagtaattct
    12541 gtagcagtgg acgtttggat tgatggatgc atgtagcacc atacacaagg gtttctacag
    12601 gaatatcgag tttttcttcc atcgccttca tctcttcaaa aggaacttca cgagcaagta
    12661 ctgctccaac tgcgcctttt ttcgcccaga aattgatttg acgtgcgctc gttactaatg
    12721 tttcagcatc ataaatatac ggtatacgca attcaggatt ttttttcatc gtataaataa
    12781 tacctgtatc accaactgtg atcacatcta cgtttatttc ttttaaaaaa gccaaatatt
    12841 caggtacatt tttcattttt tcaggatgaa tgatcccatt tacagcaata ttcgcttttt
    12901 taccatactt gtgtgctaat tctaccaatt gtcgctgttc ttctcgtgta aatgaagcag
    12961 gcagacgcaa tccaaattgc tcttcaccga aatatagtgt atctgcacct gcagcaagca
    13021 actgttctgc ttgttcaata gtttctactg tggttatgag ttcgatcata attttctcct
    13081 cctacaactt cataatagta gcatagctct aaaaactttc caatacttct tctatttaaa
    13141 gtcggatttt tgaaaattat ttcacaaaaa atcaaaataa agttcgatat attctgtcca
    13201 aatataaaaa gagattgtga caaaaatctt gtcacaacct cttaatcaag tcttactatt
    13261 ataaaaattc agctacacag gccaagtaac tatagggaac ctactgtttc ctttttaggt
    13321 ccgcgaacat tcaatgtgat cttacctttt gaagcaccta ctgttacgtg gtcacctaaa
    13381 cgaatttctc cgccaagcaa agcctcgctc aatcgatctt caatttcttt ttgaagtgcg
    13441 cgacggattg gacgtgcacc atattcagga tcaaatccag ctttcccgat cacatcgatt
    13501 gcagatggtg tgattttcac ttgaatatct tgttctttca atcgtttgat aatcgctttg
    13561 ctcatgattt ggacgattgt atggatttca tctttactca atgaatggaa gacgacagtc
    13621 tcatcgattc gattcaagaa ttcaggtcgg aaagctttct ttaactcttc taagatacgt
    13681 ttttgcatgg ctttgtgatc tttagtaaca tcttggacat tgaatccgac atttttttct
    13741 tctcgaattt gtgtcgctcc gatatttgaa gtcataatca tgattgtatt tctaaagtcc
    13801 acttttctac ctttagaatc tgttaaatga ccatcatcca atacttgcag aagtaaattg
    13861 aaaacgtctg gatgtgcttt ctccacttca tctaagagaa tcactgaata tggtttttga
    13921 cgaatttttt ctgtcaactg tccaccttca tcatacccta cgtatcctgg aggagacccg
    13981 attaatcggc ttgtactgta tttttccata aattctgaca tatcaacacg gatcagggca
    14041 tcttcacttc caaacatgac ttctgaaagt gctttggcca attctgtttt tccgacacca
    14101 gttggtccaa ggaacatgaa tgaaccaatt ggacgatcag gatctttcaa tccgcttcgt
    14161 gcacggcgga tcgcacgtga aactgctttg acagcctcat cttgaccaac gacacgttca
    14221 tgcagaagtc cttccagttc taacaagcgt tcgctttctt ttttctccaa ctgctgtaga
    14281 gggacaccag tccactccga tacaactgta gcaacatctt cttctgttac acgatcagaa
    14341 taaccttgct cctgttttgc ttcaattgca gaaacttttt gcagcttttt acgtagtgct
    14401 ttttcttgtc tgcgtaattg agcagcattt tcaaagtctt gtttttggat cgctgcttcc
    14461 ttttcttgta ctagttcatc gatttctagt ttgatcacag ttgattttgt taagtgatcg
    14521 gtttgatcca aacggacttt cgctgctgat tcatcgatca aatcaattgc tttatcgggt
    14581 agttgacgag aagtgatata acgaacagaa agattgactg ctgctcgtac agcttcatct
    14641 gtgatctcca ctccatggtg ctcttcatat cttgtacgta atccttgaag aatcacttct
    14701 gcttcttcag gagttggctc atctacttga atgcgagcaa aacgtcgctc taatgcagaa
    14761 tctttttcga tatatttttg atattcatct agggtcgttg ctccgatcgt ttgtaattct
    14821 cctcgagcta acgccggttt caagatattc gaagcatcaa ttgctccttc tgctccacct
    14881 gcaccaatta aggtatgcaa ttcatcaatg aataagatta cttgaccatc attatagatt
    14941 tcgtcgatga cctttttcat tcgatcttca aattcaccgc gatatttcgt accagcaacc
    15001 aaagatccca tatcaagcat catcaggcgc ttttcttgca tgtcttctgg cacttcacct
    15061 aaaatgattt tttgagctaa cccttcagca atggctgttt tgcctacacc aggttcaccc
    15121 accagtacag ggttattctt tgtccgtcgg ctaagaatct ggataagccg tttcacttct
    15181 ctcgaacgac caacaactgg gtccagcttg ttttcttttg ctaatttcgt caaatcacgt
    15241 gctaatgaat ctagcgtagg tgttccttgc ggtgtttgcc gtttatcttg tacatttaca
    15301 tttctacgac gattcattcc ttttccagca gaatcaccag ctagccccat tttttgtttc
    15361 agaacttgac gggcttttga aaggctgata cctaagttga tcatgattcg agtagccatg
    15421 atactttcat cttttaataa gctcatcaaa atatgttctg ttccaacttt ttgagaacca
    15481 gttttcttca cttcgctgct agctaaagca aaaacttgtt tcattcttgg tgaatatgga
    15541 agaaagactc ctttagggta agaacggata ccaccataac caatcagatg ttcaatttct
    15601 tcataaattt ccgtttcatt caaatccatt tcacgtaata cttttccagc aattccattt
    15661 tgctcgacca ctaaggctag taaaacatgt tctgaactaa ctgattgatg tttcaaccgt
    15721 tttgcttctt cttgtgcaat ttctaatact gcttgggctc tttcagtaaa aagttcttcc
    15781 atctaatctc acttcccatc ttcataactt aaacgttcca gaaatgctcg gagaatccga
    15841 gcacgtgtat gatcttcata ttcattgaaa ttcaatgtgt tttttgcaat tgcactcaac
    15901 atcaagtttc cttctttttt tgtaattatt tggtcttcat aaagtttttg aataatagca
    15961 aaagcatttt tctcactgat tgtatcatca aataactgat ttatctgctc aagtaattgt
    16021 tttttatctg aaatccttac ttttgcaatt cgtatataac caccaccacc acgtttgctt
    16081 tctactagat aaccacgttg gatggtaaaa cgggtattaa tcacatagtt gatttgtgaa
    16141 ggaacacagt taaagaggtc tgccatttca atccttcgaa tctcgatcat ttcactattt
    16201 tccagaattt tctttatata tgattcaatt aaatccgagg tattctgatg tgccatctta
    16261 ttcattccct ttgactaata ttgaccttaa ttataacgaa tttgataata gaaagaaaga
    16321 agaacgcttt ttgttaaata aatcctttag aatcaacaaa aaaaaatacg gaatatacta
    16381 ttccgtatc
//
LOCUS       DACDPS010000053        16293 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.053, whole genome shotgun sequence.
ACCESSION   DACDPS010000053 DACDPS010000000
VERSION     DACDPS010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.053"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..1781)
                     /locus_tag="IWJ07_001854"
     CDS             complement(<1..1781)
                     /locus_tag="IWJ07_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAP9333432.1"
                     /translation="MKIEIIIVHLSCIQEVKIMGIEFSERQMKILYFLIENEHTTYKE
                     LSEQLYVSTKTISKDILILKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKIN
                     IADSPEDRLTYVFAKLLNTNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLV
                     SDRNGVRIKADEKSKRRLMSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDI
                     LKKVAELLNRYMNKTQLKFTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNT
                     LILIKMIEKAFSIIVPIDEQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVR
                     IKLNEFYPDEELINNLVLHLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEE
                     IEERFCIHFNEDEIAYVTLHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFA
                     DMVEIEDVLSINELQDRKVTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQL
                     NEQTEDAFLNLLDKRFCFFTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKL
                     SSTAMNDFAMPHIEVDFIKKPVICVYV"
     gene            complement(1939..2835)
                     /locus_tag="IWJ07_001855"
     CDS             complement(1939..2835)
                     /locus_tag="IWJ07_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9333433.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(2828..3508)
                     /locus_tag="IWJ07_001856"
     CDS             complement(2828..3508)
                     /locus_tag="IWJ07_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9333434.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(3509..4291)
                     /locus_tag="IWJ07_001857"
     CDS             complement(3509..4291)
                     /locus_tag="IWJ07_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9333435.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(4284..5201)
                     /locus_tag="IWJ07_001858"
     CDS             complement(4284..5201)
                     /locus_tag="IWJ07_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9333436.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(5365..5688)
                     /locus_tag="IWJ07_001859"
     CDS             complement(5365..5688)
                     /locus_tag="IWJ07_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9333437.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            6476..9703
                     /gene="fss3"
                     /locus_tag="IWJ07_001860"
     CDS             6476..9703
                     /gene="fss3"
                     /locus_tag="IWJ07_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAP9333438.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            9816..10130
                     /locus_tag="IWJ07_001861"
     CDS             9816..10130
                     /locus_tag="IWJ07_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9333439.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            10143..10517
                     /locus_tag="IWJ07_001862"
     CDS             10143..10517
                     /locus_tag="IWJ07_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9333440.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            10527..10862
                     /locus_tag="IWJ07_001863"
     CDS             10527..10862
                     /locus_tag="IWJ07_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP9333441.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            10945..12285
                     /locus_tag="IWJ07_001864"
     CDS             10945..12285
                     /locus_tag="IWJ07_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP9333442.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            12363..13037
                     /locus_tag="IWJ07_001865"
     CDS             12363..13037
                     /locus_tag="IWJ07_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9333443.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            13270..13704
                     /gene="dcm"
                     /locus_tag="IWJ07_001866"
     CDS             13270..13704
                     /gene="dcm"
                     /locus_tag="IWJ07_001866"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP9333444.1"
                     /translation="MRFLDLFAGIGGFRLGMEQASHHCIGFCEIDKFARRSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQRFSDTRGTLFFEIARF
                     TTLLRPKLLFLENVRGLLNHEGGATFETILRTLDGLGYDVEW"
     gene            13705..14412
                     /locus_tag="IWJ07_001867"
     CDS             13705..14412
                     /locus_tag="IWJ07_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP9333445.1"
                     /translation="MLNSKAYIPQSRERIFLIGHSRDACTEQVFPIIGPSSTSDQNIR
                     NLVNINPSNRGMGGQVYGSDGVAPTLTGDEGIKIALPARDGISVAGLLPGKFEQGNRV
                     YEVTGTAPTLSTKQGGTKIMIRKNETEYQEVKPEDSVNLAFPNSTSRRGRLGKQSVHT
                     LLTGDQQAIVTDQYQIRKLTPRECWRLQGFPDWAFDRDSQVNSDSQLYKQAGNSVTVP
                     VIFDIARRLKELDVHGL"
     gene            14402..14692
                     /locus_tag="IWJ07_001868"
     CDS             14402..14692
                     /locus_tag="IWJ07_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333446.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            14951..16135
                     /locus_tag="IWJ07_001869"
     CDS             14951..16135
                     /locus_tag="IWJ07_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP9333447.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            16150..16269
                     /locus_tag="IWJ07_001870"
     CDS             16150..16269
                     /locus_tag="IWJ07_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP9333448.1"
                     /translation="MGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKGK"
BASE COUNT         5169 a         2961 c         3275 g         4888 t
ORIGIN      
        1 ttaacgtaga cacagattac tggctttttt ataaaatcta cttcgatatg cggcatagca
       61 aaatcattca tcgctgttga agatagtttt tctcttttga ttgctccctc tagtatccct
      121 tctttcgcgt aattttcttt taccaatttt ttggtaatta tatttaaaac gttcacttga
      181 ttatccttta tttggtgggt aaaaaagcaa aatcgtttat ctaataaatt tagaaaagca
      241 tcttctgttt gttcatttaa ctgtagatac tgttttactt ttctgacatc tgcccctaac
      301 atcaaaggac taacaacaat aacaggaaaa tttgtatttt caatcggaat cgtgcttata
      361 acaagttcat cagtgacttt tctatcttgt agttcattga tgcttagtac atcttctatc
      421 tcaaccatat ctgcaaaacc gttatttatt ctttgctcta ataattttga tgttccatat
      481 ccgctactac aaactaatat cactttcgtt ttgtcaggtt tgtaacgatc caaaagtgat
      541 tgaatatgga gtgttacgta agcgatttca tcttcattaa aatgtataca gaatctctct
      601 tcgatttcct ctaataaatc tacacttatc ataaatgacc ttttaaaaga atatttgatt
      661 ttgtccgtat aagggttata gatatttact cccaatttga gtcttttaac tgctgcattg
      721 agatgtaata ctagattatt gatcaattct tcatcaggat aaaattcatt taatttaatt
      781 cttactaact cttgtagctc aacacccata tctaaatagg aaagcttcgt ttgatctaag
      841 tcgtgattca tagcattttt ttcaatcgaa gtaatgtgaa tgtcaacata agcttgttca
      901 tctattggca caataattga aaatgctttt tcaatcattt tgattaaaat aagtgtattc
      961 tttgagattt cttcttcaga attattacta cgtgataaga aattgcttgt ttcaatcctt
     1021 tctatcgcaa tagcaaggtg gatagctaaa gtttgaaatt cataatctgt aaatttcaat
     1081 tgtgtcttat tcatgtatcg attaagcaat tcggctactt ttttcaatat gtccttattt
     1141 aagaaattat tcgcttcacc aatgaaattg atattcaagt ctaagagatc tgttttactg
     1201 ttagatgctg taaagccacc ccaataatag ttcacaactt ctgacatcaa ccttctttta
     1261 cttttttcat cagctttgat gcgcacgcca tttcgatctg aaactaagga catcccatgc
     1321 ttgtttagtc tttttctaac atctttcatt gtgctttcta ccgtcgacct gctcacgtat
     1381 aattcgtcag caagattttg aatctttaga gaatcattag tattcaataa tttagcaaac
     1441 acataagtta gacggtcctc cggagagtct gcgatattga ttttatgact ggataataaa
     1501 tttcgaagtg tttctttttt tccaataagc gaaacgcctt ttttaggttg aaccaacatt
     1561 tgtacatctg gcattttttg gattatttct ttcaaaatta aaatatcttt gctaattgtt
     1621 ttcgtagata catataattg ttctgataat tctttgtaag ttgtgtgctc attttcaatc
     1681 aaaaagtata gtattttcat ttgccgttcg ctaaattcga tgcccattat cttcacctcc
     1741 tgaatacagc ttaagtgtac aattattatc tctattttca tacagaagaa agttcttgat
     1801 taatgtagaa gtttctaata ttcttgatta tttcaaatca ataaaaaagc ctgcaatatt
     1861 tatatcacag gcttttttct ctattaaata tatataagat aatacatttt tctactagtt
     1921 ctcactaatc ttgtataatt aaaccctccg caacttgacg gagatggaga agatgccccc
     1981 gtctaatcta gcggtcatct ctcctcccat tccttccacc aatttttttg acaggtaaag
     2041 cccgattccg ctagattccg tatttgtgcg gctggtattt tcggtataaa aacgctggaa
     2101 gactttttca acagaggttt gcgttcctgg tttgatagga ttggccactg ttaatcgaat
     2161 gaatggatcc tcttttgaaa atgtcacctt tgcttgtcca tctgcataac gcaataaatt
     2221 ctgtatgata ttttgcagca ctcgcagcaa catgagcgga tctgctaata cagttgtatt
     2281 tatcgagtgt tcagcgaacg ctacctcgat atttttctca gaaaattgtt cataaaaaga
     2341 gaaaaatact tcttccacct ctttagaaaa agaaagcgta tggagatcca tttctttttg
     2401 cttcgtctct attcgagcaa gatcataaaa ctgatccgtc atactgagta atcttgcaac
     2461 agattgtttg atgatcgtta aatacttctc ccgtttctcc gtatcctcta cctgttccag
     2521 taattgtaca taaccgttaa tagcagtcaa aggagttctg agatcatgag aaaggccgac
     2581 gatcgatagt ctgatgtttt cttccatttc ttgaaatgct tgtccttttt gttcattttc
     2641 atccaaccat gtattcagtg catctactag gtccattaga ttcttttctc gaaattcgac
     2701 tgaaagacga ccaccatatt cttgtcgttc agaaagagtg gcgattttct tagttaatct
     2761 cttgatttcc ctttgcagga agaaaataaa acttcccata aagataacaa cgagaagcaa
     2821 cagccattta atcaaatcta aaccccactc cccacaccgt atgaatatac acagcatgtg
     2881 cctttgctag cttccctcga agattggaaa tatgcatatt cactgttttc tcactatcaa
     2941 aatatggttc ttgccaaact gtttcataaa gatttgctcg cgaaaatact tttttcggat
     3001 gttctaaaaa aagcttcagc agatcgaatt ctcttcctgt caaatggaca gccacaccat
     3061 tcacatttac atccctcgac tctttgtcca gtaagatttc cttgtaatag atgcgttgcg
     3121 acagatcttt tacttggaca gaagtctgat gacgaagttg gatgccgatt cgtaacaaaa
     3181 gttcttcgat atcaaatggt ttcgtcacat agtcatccgc tcctgctgcc aacaaggaaa
     3241 ctttatctct ttgatcgctt tttgctgaaa gaacgatgat cggaacattc gatgtctgtc
     3301 ggatttctgc taacaatgct tcacctgaaa gccctggaag catgagatct agaaggatca
     3361 aatcgacagg atatgtgctt aacaataatt ttccttccgt acctgaataa gcatcaagaa
     3421 gtttgtatct ctgttcaaga acctcctcca gtaaggaatg aacagcttca tcgtcttcga
     3481 taatcaaaat agtttccagc aggaccacct acttaatgtc agatttttgg aaaataaaca
     3541 ttcctagagc tgatgatacc acgattgtta ctagactgac cagaatcatt ttcggtgctt
     3601 gcgaataagt tgcttgttgc gcaaagtccg tatacatgag ccacgtttcc gtaaaccact
     3661 ccgttagaaa ttccaagcct ttgatttttg cgaaaacacc gggaatgaag gccgttccca
     3721 tgatataaag aaaagaaaga agcaaactga cactggtact tctagtgatg aatgtcaaag
     3781 ccactggaac agataatgtc cctaatgtca gcagaagcaa tacaggaaat acttttagaa
     3841 cataatttcc atagtttttg attccctcaa cttcaccaaa tccgaaagca agtgtgtaag
     3901 caagcatggc tagtcccgtg atcaaaacca cgccaagtgt tgtgatcaaa agcgtaacga
     3961 gaagtttcga cacataataa gtcattcgat tccgtcctaa agataacgta ttgcgaatcg
     4021 taccgttttg aaattcttcc ccaatataaa agcacgcaaa tgctgcgaaa gccagaagaa
     4081 tccatgcagc cattttttct accattaaaa tccctgttgc accgttgatg actgtatccg
     4141 ttgcttggtt taattcattg ccgtattttg tatgcatata ccattcgttc caagaataaa
     4201 aaagcggaag tacgaccatc agtcctagtg agacccacat tttacggctc ttccatactt
     4261 ttattttttc agcagttaat agattaaaca tgtggattgc ctccagttaa ttgtaagaaa
     4321 taatcttcca gtttttgccc aactaggtga ataccatcca caggaacttt tgcttctacc
     4381 aatgcttgat tgatggctgc tacttggtca attccttcaa aaagctggat aacacggcta
     4441 gattgtacaa aataatccct ataaccaaga gagtcgagaa ccgttactgc ttgttccgtc
     4501 gccgaagtgt caatttttat aaattgacga gattcttgtg ccaattcttc tttcgataat
     4561 tctttgatca agtgtccttc atgcaaaata ccatagtgag tggcgatctg tgacaattca
     4621 tctagcaagt ggctagaaag caagacggtg atgccccgtt cttttaccaa acgttggatg
     4681 atttctcgca tttctgccat ccctgctgga tccatcccat tcgtcggttc atccaaaatc
     4741 aaaaattctg gattcgtgat cagtgcattt gcaatagcta aacgctggcg catacctaaa
     4801 gagaaattct ttgctttctt tttccctgta ttttccaatc gcatcatttt cagcaggtct
     4861 tcgatttctc tatccgacac accgccgttc gctgcttgga tacgtaggtt atctcttgca
     4921 gttaattctg gataaagagc aggggtttca atcgattgac ccatacgtcg tcttgctgcc
     4981 tgcaaatccg tcgttccaaa taattcgatc gaaccttcat caatcgagat caaacccata
     5041 atagttctca taaaggtaga cttacccgct ccattttccc caatcaatcc atagatcatt
     5101 ccacgtttga tttcgattga tactttatct aatgctttgg cagcaccgta ttttttggtt
     5161 ataccagttg ctttcaagac cgtttcattt ccaaaatcca tacttatcct tctttcttat
     5221 tgatgagtga agtatagttg acgggtataa agaaagcagc cagaaatgta taaagaaagt
     5281 gtaaattttc caattgaatg tcaaaaaaaa caatcggaca atacgtgtct ttcctattat
     5341 caggcagttt gttatacgct tgtatcagtt tcctgattct ttcttttcga ccaaagtcgt
     5401 ttctctgtat tcctcttcac tgaaaatttt tgcttctttc agatcaagct tgagtaattt
     5461 taccagaccg gttataagga taactatagc aagactccct cccagaaaaa ttagactgtc
     5521 tcgcagacgt ggttgcgttg tcttccaatc aaagagcgca ccaaacagtg ctaatccaag
     5581 tggactggcc accatggtaa tcgttgttat cgttgacata agtcgtcctt gtatatgcgt
     5641 tggaacttct cgctgaagcc agaccatata aaaagcaaaa gaaaacatta atccttaaaa
     5701 acggacttat gaaaaataat acatgccgtt tgggatttac aattagctac caaataagag
     5761 gttatgacaa aagacttttc acaatccctt cttccgaata aacggtgttc tcctgccaac
     5821 tcctcggtca caagtcacaa tatttatcaa acatgagaag caatttttgt aaattccttc
     5881 ttatttcttg aagctgacca cttctgtcac aacctcagaa gcggataaat aatattgttt
     5941 gatgatactt tatctttttc ccgtttgttt atagatcgaa tgatattata gaccaagaat
     6001 cctacctatt aacatagcta aaaacacaaa aaaggcactt tccaaattgt ggaaagtgcc
     6061 agaaaagtta ggattaaggt aactgcgctc agcattgctt cgcccagtac gattctcgta
     6121 actcgtgaac tcgaacgata atcgcaatta acgttttgag aactgtgaag ctttacgggc
     6181 tttcttaaga cctggttttt tcttcgtatc attgaataag tttgaaaaac cttgatttat
     6241 aaaggttttc ccgtaaatat attttattat atttgattaa atttgaaatg tttgtgttct
     6301 aaaatgtgtt ctaatttaaa ggcagtatat ttcgtatttt agattaaatt tttaggctca
     6361 ttcattctca aatccattct attcagtcaa taaaaatttt aaaaagactt tataaacaaa
     6421 gatgttcaag cgagcatctt atttttttgt gcttaatttg aaaggagaac atttcatgta
     6481 taaaaaacaa aaaaagaaat tatgtgtatt aactattttg agtttggctg ttcaagcaat
     6541 tgttggagca attgctccta cgattacttt tgctgatgaa attacccatc cacaaacggt
     6601 aaccgtagaa ctagatttag cacatcaata tgctgttgaa ggaactttta gtgatggtcg
     6661 tccaatgtca gaagtcactg ttccgcatta tgcagtgtac aacggcttta aacaagatat
     6721 tttctgtatt gaaccaggag tcccgattta caatgagttc actcctggct atgagaaaaa
     6781 cccgttacca gatatgtccg ataaagccaa attagtttcc gttttatgga aaaaagtagg
     6841 tacagatgca gatactcata ttgtggcaca gaagatgatc tggcaagaag ttaatgggta
     6901 tacccttcac tcaataaaac gatcagatgg tagtgccgta aatatcgcag ccattgaggc
     6961 aaaaataaat caagccattg acgattatca gaagaaacca accttccata atagtacggc
     7021 taaaacagta ttagggcaat cgactactgt gacggatacg aataatctga atttgtcaga
     7081 gttcgatgag gttgtggaaa atgccgcaaa cattgattat cgtgtgaatg gtaatcagtt
     7141 gataatcacc ccaaatgcca attcaaaaga aagtggtgtg ctgacgctta agaaatctgc
     7201 tggtaccgga acacctgtcg cctataaaaa ggctggccaa cagactttaa tggccggggc
     7261 gattgataaa cctaatacct acacaatcaa aattgatgtg gaaacggagg gttctttaaa
     7321 gatcaaaaaa gtcgataaag aatcgggtga tgttgtacca ggaacggttt tccatttaga
     7381 ctttggaaaa actttacctg caaaagacgt gactactgac aaagaaggca ttgctacatt
     7441 ggatgagatt cctcatggta caaaagtaac cattaccgaa aaatcagtac cggcgcctta
     7501 tacgattgat accaccccca tgactaccac cattaaagca ggcgaaacca tttacgtgac
     7561 ttcaaaaaac gcacgggaaa aagggcaaat cattctggat aaaagcggag ttgaaacagg
     7621 gagtgatctt tggaatgaca attattcctt agctggtaat acgtttgcca ttcgcaaaga
     7681 cagtccaacc ggtgaaatcg ttcaagaaat gaccacggat gaaaacggtc atgcggaaac
     7741 accaaaagag attgctaatg cgttggaatt gggaacctat tatgtgaccg aaactaaagc
     7801 tagtcatggg ttcgtgaata cgttcaaacc tgtaaaagtc gaattgaagt atgccaatca
     7861 aaccgtagcc cctgttacca gtaacgtaaa agggcaaaat caagaagtca ctggcgaaac
     7921 cactttgacg aaagaagaca aagatactgg ggataaagca caaggtaagg cagtctttga
     7981 gggtgctgaa tacaccttgt ttactgctaa ggatggaaaa gctgttaaat ggagtgaggc
     8041 ttttaaacca gagttagcga agggaacgaa aggttctgat gaaacggtca ctttgacttt
     8101 agacgaaaag aaccaagttg ccgttaaaca tttagctatc aacgagtatt attggcaaga
     8161 aacaaaagcg cccgaaggat atagccttga tgaaacaaag tatcctgtat ccatcaaaaa
     8221 ggtggatgat agcgaaaaaa atgccgtgat tacccgaaat gttacggcaa aagaacaagt
     8281 gattcgcttt ggctttgatt tctttaaatt tgctggatca gctgcgggca ctaccgaaac
     8341 tggcttcaat gatctgacct ttaaagtgtc gccattggaa gggaccaatg aaatcacagg
     8401 tgccgaagat gaagcgacca cagcttataa cgagcaatta gggtttgatg gttatggcaa
     8461 gtttgaaaat cttccttatg gggattattt acttgaagaa gtagaagccc cagaaggttt
     8521 tcaaaagatt acgccattag aaatccgttc tacttttaag gaaaacaaag aagactttgt
     8581 gaagagtgag tatgtcttta ccattaccga acaagaccaa aaacaaccaa ttaaaacggt
     8641 gaccgttcct tacgagaagc tgacgaacaa agcattttct gttagtttga accgtttaat
     8701 gctctatgat ttgcctgagg aagaagatag tttaacttct cttgcgactt ggaaagacgg
     8761 aaacaaagaa ttaacttctc tggattctac cgagctagtt gataaattga gttacaatct
     8821 acatgaaatc aaagaagact ggtatgtggt agcgcaagcc attgatgtgg atgcgacaaa
     8881 agctgcccaa gaaaaagatg aaaaagccaa accagtggtg attgcagaaa caagcgcaac
     8941 gttggcgaac aaagagaaaa caggaacttg gaaaattcag cataaattaa ccgccgaaca
     9001 agttttaaat aaaactattg tcttattcaa ctatgtgtat gaaaataagg aagcttttga
     9061 agcaggcgat aagccagtag cgaaggatgt tagcttgaat aaccaagccc aaactgtcag
     9121 ttgtacagtg gaacaccatg tttctatcca aacaaaggcc cacctagaaa acggttctca
     9181 gacctttact catggtgatg tggtggatat gtttgatgat gtgtcgatta ctcatgatgt
     9241 actggatggc tcaaaagaag ctttcgaaac aattctgtat gctttattac cggatggtac
     9301 aaacaaagaa atttggaaat ctggcaagat tgattatgaa gtaaatgata aagaattcac
     9361 caaaactgtg cttgcgaaaa aagtagatac cggaaaatat ccagaaggaa ccaagtttac
     9421 ttttgcggaa atcaattacg ataaagatgg gaatatcaat ggaaaacaca atgaagattt
     9481 gaaagaaaaa tctcaaacct taacaccaaa agaagttcca accatactaa gtacgccgaa
     9541 acaaccggaa acaccaactg ttccaagtgg ctctcaagaa tctagtccca cagtgaagac
     9601 cttccctcaa accggggaga aaaattccaa tgttttactg ttcattggtt ttactttgat
     9661 ttttgcgacg gcgggctatt atttctggaa tcgccggaac taaggtgatg agatgataca
     9721 agtaaatcct aagaaccaac gtgcgcgacc gcctcctaaa aaagtagtcg cgctattttt
     9781 gtatccaaaa ctaacaatga aaagaggaaa agaagatgga attgaaattt gtcgtgccag
     9841 atatggctga aacctttgga aagttaagtt atgccggtga aggagaaatt ataacagaag
     9901 gatatggacg gaatactacg gtgattggtc gcagttatca tttgtattcc agtaagcaac
     9961 gggccgatga tattgaggtg gtagtagctg cggaagccgg tgaaaaggat tttgatcaag
    10021 accaaccgct aaaagccgtg aatccccatt tggttgccaa aggctatgag attgagaatc
    10081 gtgggtttac tgattacgtg ttgtatgttg atgatttagt gaaagcatag gaggaaaaga
    10141 agatgagatt aacagaaggt attgtcgtag attcaggttt aacatttggg aagttgcgtt
    10201 tttccgcatt acgccgagaa gtacggaagc aaaatgagga tggaacaatt ggtaatgagg
    10261 taaaagaacg aacctataat ttaaaatcct ctgcacaagg gcgaatgatt caagttagta
    10321 tcccagctaa tgtaccctta cgcgagtttg cttatgatgc agaggtagag ttggtcaatc
    10381 caatcgtgga taccgttgcc aattatgttt ttcgagaagg aactaccgtt aattggttca
    10441 ttaaggcgga tgatttagtc ttgaagcggc agccgaatca aggaaatcct acgaaccaaa
    10501 atgaaagaaa gaaataggtg aagattatgg aaattaatgt tgaacatatt ttagattgtc
    10561 tcgatcaata tggaaaaggc gagctgactg aggaacaact cgttaaagca ttaacctctg
    10621 atgaaaaaat gtttctgatt atacatcaag gtttacttgg agaaaatagc gacagtgaag
    10681 aagactctga tgtactagac tggttaggag aagaagagtc tttctttata gttgtcgaag
    10741 taaatgaaaa cttatgtcgg caggcagaat ccattttgga agaaattggt gtggagatgc
    10801 cggatgctat tgaaggcttt ttaaatcaat tggttgaaac gaaacaactc ccctcctcgt
    10861 agcagtaaat gactagcata actttttgaa attgcctaat gaaatcctat tgggcttttt
    10921 cgattggagg tggaaaccat caaaatgtac aaaggacatc gcatacgcgc aggagatcag
    10981 cacttggttt atcactttgt tcttggctgg ctactcgctc tgttcatcgg ttggatgagc
    11041 gtcttttatt ttcaagagtt tagacaattt gatatttcta agctatcact ttctactatt
    11101 gaaatcgttt ggtctataaa agatttagta tgtctgcttg gaagtctcgc tttttcagga
    11161 gctatgatat tgctttatat tcactttttt cttgatcatt ggcgaagctt gtggcatcga
    11221 caaaagctag ctcggatgat tctagagaac cattggtatg aagtgaaaca aactcaaagt
    11281 gaaggctttt tcaaagattt gaatagtagc cgaaccagag agactatcag ttacttcccc
    11341 aaaatctatt atcgaatgaa agatggatta ctgtctattc gcgttcaaat ttcacttggg
    11401 aaataccaag atcagctttt aaaattggaa aagaagttag aaagcggact gtattgtgag
    11461 ttagtagaaa aggaactcaa agactcctat gtggagtata ctttactgta tgatatgatc
    11521 gccaatcgaa ttgggataga cgaagtagtg gcagaaaatg ggactctgcg attgatgaaa
    11581 aatcaagtat gggcttatga ttctttaccc catatgttaa tcgctggtgg cacgggtgga
    11641 gggaagacct atttcctcct caccattatc gaagcactat tgaagtcaga tgcagaattg
    11701 tttatcctcg atcccaaaaa tgcagattta gctgatttag gtacggtgat gcctcatgtt
    11761 tattcacaaa aggaagaaat ttctgcttgt gtggaagatt tctatgaacg tatgatggct
    11821 cgtagcaagg caatgaaaga aatgtctaac tacaaaacag gagagaatta tgcctatctt
    11881 ggacttccac ccaacttttt aatctttgat gaatacgtgg cttatatgga gatgttaacg
    11941 acgaaagaaa gtgcggtgat tttgaataag ctcaaacaaa tcgtgatgtt gggtcgtcaa
    12001 tctggtttct ttctgatttt ggcttgccaa aggccggatg cgaaatattt aggagacgga
    12061 atccgcgatc aattcaactt tcgcgtggct ttgggtcgta tgagtgaact cggatattcc
    12121 atgatgtttg gggaggtcga aaaaaacttc tttatgaagc acatcaaagg tcgaggttat
    12181 gtggatacag gtggcagtgt gattagtgag ttttatacgc cacttgtacc aaaagggtat
    12241 gatttcttag attcaattag taaattagaa aagatgaatg attgagtgat cttcaatact
    12301 gactttatac ttcaagtaac gtataatact tttatatagt aggaagaaag gggataatta
    12361 atatggaaag taatgattct tatttagagt atgcaaaagc aaataaggat aagtttattt
    12421 cttatattat caaagggaaa tcccccgaag ttggacgaga cgcagttttt atggcaggga
    12481 gtcctggggc tggaaaatca gaggttgcca ttggattagc tgagaattat gataaccatg
    12541 tgattattga tgcggattac tttcgcaccc agtttcctga ttataatgga aaaaactcta
    12601 gtctctttca aaaagcatct tcttggttgg tagagcaggc atttagatat gtcttagatc
    12661 atggctattc ttttattctg gatgcaactt ttgcaatatt aagtgctgaa aaaaatatct
    12721 cgcgggcgtt gaaaaaacat tttcgagtga cgattttcta tgtttatcaa gaccctaaaa
    12781 tcgcttggaa atttacacaa gaacgtgaaa ttgcagaggg acgtcatgtg ccgaaaaagg
    12841 catttattaa cgcttttttc aaatcaagag agaacattgc aaaagtaaag agtaaacatc
    12901 cagatgtatt gcttcatatc ataataaaag attatacgaa taatatttcc gaagttcatt
    12961 atgctacgga taatctagaa ttatttttac cactagggta tagtaggaaa gatttggagg
    13021 ttcaattaca tgactgagaa agaacaagta acgaaaattg taaagaaata caataagagc
    13081 attgcggatt tatcggaaaa tgctactgca aaagagttca aaacggtgat taaatatgta
    13141 gccgatcagg ccaatgaaaa acaacggaaa ttagttggat taaataaaaa atagtaggac
    13201 aaattaataa tgtatgagcc aagagcaaaa tgttcttggc ttttttcttt gcaatgaatg
    13261 aggtaatgta tgagattttt agatttattc gccggtattg gtggctttcg tttaggtatg
    13321 gaacaggcga gccatcattg tatcggtttt tgtgaaattg ataaatttgc tagacgtagt
    13381 tataaagcaa ttcatgatac aagtaaggag gtggaaatgc atgacatcac aagtgtatca
    13441 gatgagttta ttcaatccct cggaccagtg gacatccttt gcggcggatt tccgtgccaa
    13501 gctttttcaa ttgcgggaaa gcggcaaaga ttttccgata ctcgaggtac tttattcttt
    13561 gaaatcgctc ggttcaccac tcttctccga cctaagcttt tattccttga gaacgtccga
    13621 ggattgctca atcacgaagg aggggctacg ttcgagacga tcctccgaac gctggatgga
    13681 ttggggtatg acgtggagtg gtaggtgctt aactcaaagg cctacattcc ccaaagccgg
    13741 gagcggatat tccttatcgg acattctcga gacgcatgta ccgaacaagt atttcctatc
    13801 attggaccgt cttcaacatc tgatcaaaac atcagaaatt tggtaaatat caatccttcc
    13861 aatcgaggaa tgggtggaca agtctatggt tcagatggcg tagcgccaac cttaacaggt
    13921 gatgaaggaa tcaaaatcgc actgccagca agggatggaa tctctgtagc aggactatta
    13981 ccgggaaagt ttgaacaagg taatcgagtt tatgaagtta ctggaaccgc ccccaccttg
    14041 tcaacgaaac aaggtggtac aaaaatcatg attcgaaaaa atgaaacaga atatcaagag
    14101 gtgaagccag aagacagtgt gaatctggct tttcctaatt ccacaagtcg ccgaggtcgt
    14161 ctgggaaaac agagtgttca taccttacta acaggagacc aacaagcgat cgtcacggat
    14221 cagtatcaaa ttcggaagct aacacctaga gagtgttggc gacttcaggg ctttcccgat
    14281 tgggcttttg atcgagattc tcaagtcaat tcagatagtc agttgtataa gcaagccgga
    14341 aactctgtaa cagttccggt catttttgat attgccagaa gattaaaaga gttggatgta
    14401 catggtttat gatgagttga ttggagaaat ttattgggtg attggaaaga ttcagtcaga
    14461 cccagaatta gaagaacaat tgcgacggtt aaaatttgat attcgcaaga atggtgtcaa
    14521 agtcgctggc gatccatatt taatgaatga ggagacggat gctcgaattg aaataaatca
    14581 agtaattgca gaatttgagc ggattgcgga ccaagcaaaa gaaccagata ttcgtcaata
    14641 cttgttagaa atgaaagcag agttggaagt aaatggtatt acagatgagt aacctaaacc
    14701 aacacaactg actgttaggc gctgaccgcg gaggattcgc gaagcagaaa tcctccgtgg
    14761 tgctgtgcct gttcatggac ggcgttagcc gaccatgatg tctcaccccc gtatctaaca
    14821 ggggggtaca aaaacacaaa aaataagtaa gcaaccatta agaatgcttg ttgtatcaaa
    14881 gatagtaagt aatttgtata ggtgttaact catttgaaaa agttaacacc ttgaatttgg
    14941 aggtgagaac ttggcacaac ggaatttaga ttatcggcta ttaaaagacc gacgcaacga
    15001 atacggcgtt tcacaaaata aattggctac ggcttgtgga cttagtcggc cgtacttaaa
    15061 tcagatagaa aatggtggcg taaccgcatc tataaaaacc atgaggaaaa tttttgacca
    15121 actggaaagc tttaatcccg atttgccttt gacgttactg tttgactatg taaggattcg
    15181 ctttcccaca acggatgcac gaaaaattat tcaagaaatt ctccatttga aatttgagta
    15241 tatgctccat gaagattatg ccttttactc ctatcaagaa cagtatgtca tgggtgatat
    15301 tgtggtgatg ttgtctcatg aagaagataa aggtgtgctt ttagaattaa aggggcgagg
    15361 ttgccgacaa tttgaaactt ttttactcgc tcaaaagcgt agctggtacg actttttcga
    15421 ggattgtcta aaggccggtg gcgtgatgaa acgcttggac ttggcaatca atgatcgagt
    15481 ggggctattg gatatacctg agctaacgaa aaaatgtcag aaggaagaat gtatctcctt
    15541 gtttcgtacc tttaaaagtt atcgttccgg ggaactttta aaggctgatg aaaaggacgg
    15601 catgggcaat accttatata tcggcagtct caagagtgag gtttatttct gtttgtatga
    15661 aaaagactat gaacaatata tcaagttagg gattccatta gaccaaactg agacgaaaaa
    15721 tcgttttgag attcgactga aaaatgaccg tgcttttcat gcgattcaag atttgttaaa
    15781 aggtcgtagc attgagagca ccacgttttc cattatcaat cgttacttgc gatttgcgga
    15841 taaagtagaa ggtaaaaggc gaaccaactg gcccttgaat gaacaatggg ggcgttttat
    15901 tggacggaat cggaaagaga ttcaattaac ttccgagcca aaaccgtata ccattgaacg
    15961 aaccttgaat tggctaggcc gacaagtcgc ccctacgtgg aaaatggcta aggagttgga
    16021 tcgattgaat cggacaacct atatccagga tatggtacag aatgcacgct tatcagaccg
    16081 acataaaaag attttggagc aacaaagtat ggcaattgaa aatttgatta tatgaaaaga
    16141 ggaatcgtca tgggaattgt aaaagatatt tttttggtat ttggtggcag tatgcttggt
    16201 gttactgtga tgtgcttgat gaatgcaagt gcagcagcgg atcgagcaat ggaaaagaaa
    16261 ggaaagtgac aagatgaatt tcggacaaaa ttt
//
LOCUS       DACDPS010000054        16206 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.054, whole genome shotgun sequence.
ACCESSION   DACDPS010000054 DACDPS010000000
VERSION     DACDPS010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.054"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            16..318
                     /locus_tag="IWJ07_001871"
                     /pseudo
     CDS             16..318
                     /locus_tag="IWJ07_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320930.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase"
     gene            566..784
                     /locus_tag="IWJ07_001872"
                     /pseudo
     CDS             566..784
                     /locus_tag="IWJ07_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296656.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(913..2121)
                     /locus_tag="IWJ07_001873"
     CDS             complement(913..2121)
                     /locus_tag="IWJ07_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEnfa110 family transposase"
                     /protein_id="HAP9333449.1"
                     /translation="MNCVGIDVSKGKSMIAVMRPFGEVVVSPFEVRHTANELSELAGL
                     LKSLDGETRVVMESTGNYHAPVAWLLHDAGLYVSVVNAMLVHDYGNNSLRRAKTDKKD
                     AVKLANYGLDHWLTLPRYIPEEDTRLMLKICYRQYQQYSKVQTMLKNNLISLLDTTFP
                     DANRLFTSPPRADGSEKWVDFVATFWHCECVCGRSEKAFTTQYQKWCRKHGYNFSEDK
                     ALDIYASACRHFGVIPKTDTAKLLVEQAISQLQTTSSALAALKQEMQSLAASLPEYPV
                     VMGMFGVGPTLGPQLLAEIGDVRRFHSKKALVAFAGIDAPPYQSGQIDVRSRSISKRG
                     SASLRRTLFLVMSVILQCAPIDEPVYQFMDKKRSEGKPYRVYMMASANKFLRIYYASV
                     KAYLESLEHD"
     gene            2411..3376
                     /locus_tag="IWJ07_001874"
     CDS             2411..3376
                     /locus_tag="IWJ07_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004605046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9333450.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKNLTLKAKGLLSQMLSLPEDWD
                     YTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDAKGKMADMVYTIYEQPVLENP
                     VLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPSP
                     TPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVDL
                     MLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYLR
                     AMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
     gene            3398..3610
                     /locus_tag="IWJ07_001875"
     CDS             3398..3610
                     /locus_tag="IWJ07_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP9333451.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            3685..4161
                     /locus_tag="IWJ07_001876"
     CDS             3685..4161
                     /locus_tag="IWJ07_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HAP9333452.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            4158..4658
                     /locus_tag="IWJ07_001877"
     CDS             4158..4658
                     /locus_tag="IWJ07_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003525523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG"
                     /protein_id="HAP9333453.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPS
                     APVRAV"
     gene            5218..7035
                     /locus_tag="IWJ07_001878"
     CDS             5218..7035
                     /locus_tag="IWJ07_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP9333454.1"
                     /translation="MRNPIHVLKSLEEKASVSNYKYERLYRNLYNPEFYLLAYANIAK
                     SQGSMTQGVDGQTLDNMSLPRINRIIESIRNRTYQPKPAKRKYIPKKNGKLRPLGITS
                     TDDKLVQEVVRMILEAIYEPTFSNNSHGFRPKRSCHTALTQVKKNFTGVTWIVEGDIK
                     ACFDNFDHHVLVELLRKRISDEAFIGLIWKFLKAGYMEQWQYNCTYSGVPQGSGISPI
                     CANIYLSELDNYMQEYKEKYDCEPERRRTTREYERASRRYRKARKALMGAEKSTPELV
                     KEFKDSRRKKMNQHYYNPFEEGFKKIQYNRYADDFVIGVIGSKKDAEKIKEDVKIFLQ
                     EKLHLEMSEEKTKVTHSSKPVRYLGYDFKVIHSKNMKRCKNGDMKRVWYGKVFLYMPK
                     EKWIKKAMERGAIQVKRNNDTGKEMWRPMPRKDLMNRSDAEIVSTFNSEIRGLYNFYR
                     IAENVGALHKYYYMVRYSMLKTLAGKHRTNVSVIKKRHMVNGVLRIPYDTTKGRKYCE
                     FYHDGFRKHSDGYDNVADVMPSYRKYDSRHTIVNRIKAGVCEICGEHADYLCMHHVRT
                     LKSLKGRDIFEQKMLKMRRKSLALCPDCFELLHETKESR"
     gene            7144..8469
                     /locus_tag="IWJ07_001879"
     CDS             7144..8469
                     /locus_tag="IWJ07_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006771541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP9333455.1"
                     /translation="MQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTIN
                     FSKSMKYNPFVYIHSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIW
                     YEAPAEEMNFSTLLEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLL
                     SAGKTRSSILISCGARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFN
                     FILAMLQSQLINLLCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREI
                     SASIILQSQSQLKAIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTS
                     ENRGSQTSHGLNYQKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYK
                     YLADADKKNTFDVDRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            8546..8707
                     /locus_tag="IWJ07_001880"
     CDS             8546..8707
                     /locus_tag="IWJ07_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003509345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333456.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKHLMSSM"
     gene            8748..9128
                     /locus_tag="IWJ07_001881"
     CDS             8748..9128
                     /locus_tag="IWJ07_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnpV protein"
                     /protein_id="HAP9333457.1"
                     /translation="MTISCKAKGDTMKIEYTKVGDYYLPNLYYPEETRPIGLWGMLRK
                     EYLKEHKSGTYTYLLLTARLDSYLADLNEQAQERFELIETQMRSAEGVSEELKRQNPM
                     EWLCHCNNIRNRAAEIIKQELIYV"
     gene            9143..9424
                     /locus_tag="IWJ07_001882"
     CDS             9143..9424
                     /locus_tag="IWJ07_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333458.1"
                     /translation="MRRELIESYYEEYQRELERFLESDPEMFTLEAEIHHYIEILEPV
                     LRDGGQEMWQTLDRIISAKNAMEGIVAKEMYIRGFLDYERLVCEKDSKI"
     gene            9683..10465
                     /locus_tag="IWJ07_001883"
     CDS             9683..10465
                     /locus_tag="IWJ07_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAP9333459.1"
                     /translation="MKVKNADEFYKILERKAIYPVFQPIVNLQTGDVAGYEALSRIDR
                     HDTTLMISDLFVIAEQVGCVWKLEKLCRNKALKAAANKPEHAKLFLNVDGNIIQDKSF
                     IQGFTNRKAAKAGVPSCDIVFEITERSDIENYQILQQIMKHYADQGYEIALDDVGAGY
                     SGLNRVVNTSPNYLKVDIELVRDIQKHKKKEIMMEFLLHYCNETGAILIAEGIETEAE
                     LECLCRLGVHYGQGYFLGRPDRTFKNVRNEAMLVLRRLNNKS"
     gene            10638..12356
                     /locus_tag="IWJ07_001884"
     CDS             10638..12356
                     /locus_tag="IWJ07_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HAP9333460.1"
                     /translation="MNENVKVVFGLIGGLALFLYGMNSMSDALQKAAGERMKKILGFL
                     TRNPIMGALAGALVTAVLQSSSATTVMVIGFVSAGLMSLPQAISVIFGANIGTTMTAQ
                     LMAFKISNYIYPIIFVGFILNFVSKKEKVKNIGMVIFSFGLLFEGIEIMGEVMKPLAG
                     SPVFVDLMGKVSSIPVLGVVLGAVMTLVVQSSSATIAVLQNFASQAGPDGVSSVIGLT
                     GAIPILLGDNIGTTITALLASIGQSKNAKRTAIAHSIFNISGSCVFIFLVPWFAKFVQ
                     FISPKGNEIDVISRQIANAHTTFNIVCTLVWLPLIPLMVKIVTTIIRGNDKTEKAAFE
                     PKYLDMKVIEQPAAAMVLVSKELNRLGELAESLLSDLKTAIVADGDSKTHGSFIENLE
                     IVHQLQDSVTEYITRLFASGNLTEQQSEQTAGLLYVNNSIQRIADRCEDIDQICEKVN
                     NNGKRLTSEASSEMEDCIDIIQQLLKKAMEAIRKGDAVVAETVFKNKKKMHKAEKKYS
                     KAHLNRVKNEVCDASMTRYFSGIMYNFDRMADNCVSIAEEACDNVAFINLDEETGKSM
                     MERGAV"
     gene            12358..13212
                     /locus_tag="IWJ07_001885"
     CDS             12358..13212
                     /locus_tag="IWJ07_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333461.1"
                     /translation="MVKKKNIRKSALLLIMIMLATGITACGTEEEPLPDLSAMGAVTA
                     VSREEGSGTRAEFENLLKLPESDTGIVVDSTEKVLKKVEEDKNAVGYVAYSSATDTNG
                     KILQINGVLPSEKTIDNNSYPLCRDYYLAYNGELTDVEQDFLTYVKSKGQDIVKQYCI
                     QADSTTTFLSDKSEGKILIEGSTSMEPMVKALADDYQKQNPNAEIEVKATDSSRGITA
                     VISGECDFAMSSRELKDYEAELLETKVIGKDAIAIVINEENPLENLTIKQLTGLYNGT
                     YKNWDDLS"
     gene            13888..14208
                     /locus_tag="IWJ07_001886"
     CDS             13888..14208
                     /locus_tag="IWJ07_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333462.1"
                     /translation="MPGKEQFMTELKMKEMQLINWFGCPNLIYTRERIHLVAMLTTDE
                     EMKKNLYRVCRFLAREEIAYRYPIMYEMIRKIFSPDDTNPPEACAMFMPDDETDDEEY
                     AMAA"
     gene            14232..14642
                     /locus_tag="IWJ07_001887"
     CDS             14232..14642
                     /locus_tag="IWJ07_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP9333463.1"
                     /translation="MNKVVLMGRLTKNPEIKYAGKDNDMAVARYTLAVNRRYKRDGEQ
                     EADFISCVTFGKSAEFAQKYLHKGMRIVIGGRISTGNYKDKDGKTIYTTDVIVEEHEF
                     AQNKDNGVGADSSETPKTDKDGFMEAPEGEVPFD"
     gene            14789..14995
                     /locus_tag="IWJ07_001888"
     CDS             14789..14995
                     /locus_tag="IWJ07_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005341250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9333464.1"
                     /translation="MGVSYNRLWKMLIDKNMKRIEMQYLTGISGNILARMGKNQYVSM
                     ETIEKICKKLDCTVDEMLEFTDDE"
     gene            15342..15863
                     /locus_tag="IWJ07_001889"
     CDS             15342..15863
                     /locus_tag="IWJ07_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9333465.1"
                     /translation="MIRKAILKDVNSIEDTYNEHFQYELNHTAFTVFKKGVYPTKDDA
                     ERAIYAGALFVYEENGTIVGSIIIDKVQPIEYATIPWKEKLSEDEVMVIHLLMVRPSM
                     SGKGIASSLIKFATELAQKNSCRALRLDTGSQNIPALSLYQKNGFEIIASASKKVGDV
                     IAHKNHLFLEKIL"
     gene            15921..>16206
                     /locus_tag="IWJ07_001890"
     CDS             15921..>16206
                     /locus_tag="IWJ07_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9333466.1"
                     /translation="MIRELRKVDINKVAEIWLDTNIKTHYFISAQYWKSNFELVKELL
                     LQATVYVYEDKQEIQGFIGLSNEYIEGIFVSAEMQSQGIGKILLNYVKGKR"
BASE COUNT         5127 a         3183 c         3985 g         3911 t
ORIGIN      
        1 gaataaagaa agaagttgaa aacgatacct gattacaaaa aaataacttt tataagttct
       61 ataacgttga ttatattttt aattcttatt cttctcgtcg tcttggttcc ccgaattttt
      121 ggtttgactc cttatattat tgaggacaaa gaaatgagtc catattatgc acaaggaaat
      181 ctgatatatg tacgaccgaa aaatacaaat gagattttag tcggagatat tattacatat
      241 tatgaaaatt caggaaaaaa aatctcgacg cgtcgcgtaa tagccgttga aaacaaccac
      301 gaagattttt cctcaaaatt ttaggttatc gctgaaagcc ccggaaatac gggcatttcg
      361 caggatatga gaactcaatt tactaccaat ttactacttt cggattgagc cgcatatatg
      421 ctgaaccata ctgctaatac ccgccacccc aaatacggca catcataaat tgaatacgac
      481 accgtaaacg cggcgttcct ctttcccgtc atgggagaac aggacgccgc ttttttatgc
      541 ccaaatagag aaaggaggcg acgccatgct atttacttcc ggccctgacc gggcgtttga
      601 acggctgatg caggaaaagc ccggtttcga tcactacgac ggcggcgggg cggacgcgcc
      661 ggaggattgc ggcacctgcc gattttaccg cccggattgg aaatatcaat tctgccagtt
      721 tgcagagtgt ccctatcagc cgggcaagct gaccgactat gcagcggtca aattcacagt
      781 gaaagacctg ctaataaaag tcaagtagaa atttcagatt tttagggagg cgaaaaaatg
      841 caatacaaaa tcaagccgct gagcatctca aaattaaaac ggcggccagc cagatggtat
      901 agagtgtaaa aatcagtcgt gctccaggga ttccaagtag gctttcacag aagcgtagta
      961 gatgcgcagg aacttgttgg cagaggccat catgtagaca cgatatggct tgccttcaga
     1021 gcgtttcttg tccatgaact ggtacaccgg ctcgtctatt ggagcacatt gcaggatgac
     1081 gctcatcacc agaaagagcg tcctgcgcag tgaggcagat cctcgcttgg aaatgctacg
     1141 gctacggaca tctatttgac cggattggta gggcggggcg tcaatacccg caaaggcaac
     1201 caacgctttt ttggaatgaa aacggcgtac atcgccaatt tcagccagga gttgagggcc
     1261 gagggttggg ccaacaccaa acatccccat cactacagga tactccggca gagaagctgc
     1321 cagggattgc atctcctgtt tgagagcagc taacgcggaa gaagttgtct ggagttgaga
     1381 aatggcctgt tctaccaaaa gttttgccgt atccgttttc ggtatgacac cgaagtgtct
     1441 gcaagcagag gcataaatat ccagcgcctt atcctcgctg aaattgtagc cgtgctttct
     1501 gcaccacttc tggtattggg tagtaaaggc cttctcagac cggccacaaa cgcactcgca
     1561 atgccaaaaa gtagcgacaa agtccaccca cttctcgctg ccatcggcgc ggggcggact
     1621 ggtaaacaag cgatttgcgt ctgggaaggt ggtgtccagc agggagatca ggttattctt
     1681 cagcatggtc tgaactttgg aatactgttg gtactgccgg tagcagattt tcagcatgag
     1741 tcgggtatcc tcctccggga tatatctcgg caatgtgagc cagtggtcaa gaccgtagtt
     1801 ggccagcttt acggcatcct ttttgtcggt tttggcccgt cttaaactgt tgtttccgta
     1861 gtcatgcacc agcattgcat tgactaccga gacataaagg cccgcgtcgt ggagcagcca
     1921 ggccaccgga gcatggtaat tacccgtgga ttccatcacc acacgggtct cgccgtccag
     1981 gcttttgagc agtcctgcca gctcgctcag ttcattggcg gtgtgacgta cttcaaaggg
     2041 tgaaaccact acctctccga agggccgcat gactgcaatc atgctcttac ccttggaaac
     2101 atcgatgcca acgcagttca ttcacattcc tccaatacaa agaatctgca accggaatcc
     2161 atcttttctc gctgccgatt caatctattg tgtgacacga actctccgac atccggtggt
     2221 tcaacctgct taaatcgaac gctgcaacga gaggatggct aacagtcttt cacacggaca
     2281 tacaagccca aggagtgatt ggtcagccag ttgctcctct cattgtagct taggcacgag
     2341 atggagggaa agccggactg gctgtccggc tttcctgtcc aaatttattg taataggagc
     2401 gaacaacgct atggcagttt tccgtgtgga aaagaacaaa ggctatacgg tgatgtcaaa
     2461 tcaccacctg cggaataaga acctgacctt aaaggccaag ggcctgctgt cgcagatgtt
     2521 gtccttgccg gaggattggg actataccct aaaaggactg tcctatatca accgggaaag
     2581 catcgacgcg atcaggacag cggtatggga gcttgaaaaa gctggatata tccggcgtga
     2641 gcagggccgt gacgcaaaag gcaaaatggc cgatatggtc tataccatct acgaacagcc
     2701 ggtattggaa aacccggtat tggaaaatcc aacatcggat aacccggtgt tggaaaatcc
     2761 gacatcggat aatccggcgt cggaaaatcc aacgcaatta aataaagaag tacaaagaac
     2821 tgacttatca aaaaaagaaa aaataattac agatggacaa agtacccatt ccattcctat
     2881 cccatccccg acccctgccc ctatggaggg cgaggcagcg gctacgccgc cggaacggaa
     2941 aaggacagga aaggatgagg ctgtacggat ttatcgggaa atcattttgg agaacatcga
     3001 gtatgcctat ctcattcagg acaggagcat tgaccgtgaa cagcttgacg aaatcgtaga
     3061 cctgatgctg gaaaccgtct gcaccgcccg aaagacaatc cgtgtcgccg gggacgacta
     3121 ccctgccgag ctggtgaaat ccaagtacat gaagctgacc ggcgaacata tccgctttgt
     3181 ccttgactgt ctgcgggaga acacaacgaa agtccgcaat atcaaacaat acctgcgggc
     3241 tatgctgttc aacgccccca gcaccatttc caactactac acggcccttg tcgcgcacga
     3301 catggcacag cccgattggg gaaagcctaa atccggcctg ccggactatt cctgcggcga
     3361 gggcgaaagc ctgtaacgag aaaaggagga cgaccctatg aagcagggcg cattgatttt
     3421 tgacgagcga acagaccgtt acgacatccg ctttgatctg gccgactatt acggcggcct
     3481 gcattgcggg gagtgtttcg acgtgatgat cggcggcaaa tggaggccca cccgcattga
     3541 aatggccgaa aactggtatc ttgtcggcat ccgggccgac gacctttccg gcctgcgggt
     3601 gcggatttga cctatatcta cctgcggcgt ctggtggttc cgccttagac gccgcttttg
     3661 actttctgac aaggagggat gcgattgcag gaagaagtta ctcaaaagac cattgccctg
     3721 tccatgaaaa cgggcaagct cacggctcaa gtgctgcaag cggcgctgaa aaagtacctg
     3781 caacaccggg ccaagggcaa aaacacgctg caccacggcc agcagagcct aaagcagctt
     3841 aaaaagcatg gggctgatct ctccaacatt gagatcaccg aggccaacat tggagcgttc
     3901 aagccatgcg ccaagaaata cggcgtggat tttaccttac gaaaagataa gaccacccag
     3961 ccgccccact acgttgtgat tttcaaggcc aaggatgcag ataaccttga tctggcgttt
     4021 cgggagttta ccgccaagac gctttccaag gagcagcggc cctctatccg caaggttttg
     4081 tccgctgtga agcaaaaggc ggcggcccag accaagcagc gggccaagga gaaaatcaag
     4141 gaaagggggc tggaactgtg aagcctgacg taaagaaatt gctgatcctg aacctcccgt
     4201 atctgatttt tgtgtacctg tttgcgaaat gcggggaggc gtaccggctg gccgctggtg
     4261 ctgacctgtc ggggaagctc ctgcattttg cggatggttt tgcggcggct tttgccaacc
     4321 cgctgccaag cctgcacctg tttgacctgt gtatcggtat cgccggtgcc gtgatcgtgc
     4381 ggctggtggt gtactgcaag agcaagaacg tgaagaaata ccgcaaaggc atggagtatg
     4441 gcagcgcccg ctgggggacg gccaaagaca tagcgcctta catcgacccc aagtttgaaa
     4501 ataatatcct gctcacccaa acggaacggc ttaccatgac cggcaggccc aaagacccca
     4561 agacagcgcg gaataaaaac gtgctggtga tcggtggcag cggttcaggg aaaacgagat
     4621 ttttcgtgaa acccagtgcg cccgtaaggg cggtataaat cctaccttga gcaagtagta
     4681 ggtaaaagta tcaagcggca ttgtgccgca acctatcatt ccccgtctta ccccaaaagg
     4741 gaaaccggag ggcgaccata gcatgacgga ggacacgggg cgctgaagcc tcagaagcgc
     4801 cggcgtgacg gaatgaaaat ccacgtatga ggatggaagc tagactgctt gaatgacagc
     4861 ctgaaatcgg gaccaagagc gtacccctga cgtagagagg ttgtactcgt cagtgttcct
     4921 gacagtcgca ttgtttggct atgcggctgc tgatactcgg agcaggttca gccacgggaa
     4981 agtggaaaaa ggcgaacgtc acataccgac aacgtggaac gcttgtttat accgtactaa
     5041 acggggattg cctaaagtga aatgccgaaa ggctatgaaa acgctgaaat gcggggttct
     5101 gaatattcac catggcaaca gagttcccat agtagtctgc ggacgggaaa gccgtctaca
     5161 tggcaaaggg gaacagtgaa tcattttcaa aacagaaagg atggtgtgtg agacactatg
     5221 agaaatccga ttcatgtctt gaaaagcctt gaagaaaagg caagtgtaag taactacaaa
     5281 tatgaaagat tatatcgcaa cctatacaat ccagagttct atctccttgc atatgcgaac
     5341 attgcgaaat cgcaggggag catgacgcag ggggtagacg ggcagacgct ggacaacatg
     5401 agcctgccga gaattaaccg gattattgaa tccatacgca acaggacata tcagccaaag
     5461 cctgcaaaaa gaaagtacat tcctaaaaag aacgggaaac ttcgcccgtt gggaatcact
     5521 tctactgatg ataagttagt gcaggaagtg gtcagaatga ttcttgaagc aatctatgag
     5581 ccgacattca gcaacaattc acacggtttc agaccaaaaa gaagctgtca cacggcactt
     5641 acgcaagtga agaaaaactt cacaggtgtt acatggattg tcgagggaga cattaaggcg
     5701 tgctttgata acttcgacca tcatgtgttg gttgaattac tgcggaaaag gatttcagac
     5761 gaggctttta tcggtctaat ctggaagttc ctgaaagccg gatatatgga acaatggcag
     5821 tacaactgca cctactccgg tgttccgcag ggcagcggta tcagtccgat atgtgcaaac
     5881 atctacctca gcgaactgga caactatatg caggaataca aagaaaagta tgattgcgag
     5941 ccagaacgca gaaggacgac cagagaatac gagcgggcgt cccggagata cagaaaggca
     6001 cgtaaagcgt taatgggcgc agaaaaatca actcctgaac tggtaaaaga atttaaggat
     6061 tccagaagga agaagatgaa tcagcactat tacaatccgt ttgaggaagg cttcaagaaa
     6121 atccagtaca accgttacgc cgatgatttt gtaatcggcg ttatcggctc caaaaaggac
     6181 gcagaaaaaa tcaaggaaga tgtaaaaatc tttctccaag aaaaactgca tttggaaatg
     6241 tccgaggaaa agacgaaagt cacccattcc agtaagccgg tacgctatct ggggtacgat
     6301 ttcaaagtaa tccattccaa gaacatgaag cgttgtaaaa acggcgacat gaaacgggtg
     6361 tggtatggaa aagtattcct gtatatgccg aaagaaaaat ggattaaaaa agcgatggaa
     6421 cggggagcga tacaggtgaa acgcaacaat gacacaggca aagaaatgtg gcggcctatg
     6481 ccaaggaaag acctgatgaa ccgcagcgac gcagagattg tgtctacttt caattccgaa
     6541 attcgagggt tatataactt ctatcggata gcagaaaacg taggcgcatt gcacaagtat
     6601 tactacatgg tgcggtacag catgttaaaa actctggcag gaaagcacag aacgaatgtc
     6661 agtgttatta agaagcggca catggtaaac ggagtactga gaatcccata cgatacaact
     6721 aagggacgta aatactgcga attttaccat gatggattca gaaagcacag cgacggctat
     6781 gacaatgtgg cagacgttat gccgagttat aggaaatatg acagcaggca cacgatagta
     6841 aaccgcataa aagccggagt atgcgagatt tgcggggaac atgcagacta tttatgtatg
     6901 caccacgtaa gaacgctgaa atcgctgaaa ggcagggata tatttgagca gaaaatgctg
     6961 aaaatgagaa gaaaatctct ggctctctgc cctgactgct ttgaactctt acacgaaacc
     7021 aaagaatcaa ggtgattcat ggaaagccgg atacgctgag aggtgtacgt ccggtttggg
     7081 aggcggctcc ctgaaaccta ctgatgaaag tcagcaaggc gcagggtgcc tacctcatac
     7141 ctgatgcaat gctttccgac cacggactat ccgacttcgt ttgtggtgac agatccgaaa
     7201 ggcactttgg ttctggaaac cggcaggatg ttccagcagg cgggctaccg cattaaaatc
     7261 ctgaatacca ttaacttttc caagtccatg aagtacaacc cctttgtcta catccattcg
     7321 gaaaaggacg tgctaaagct ggtaaatacc ctgatttcca acaccaaggg cgagggggaa
     7381 aagtcagcgg aggatttttg ggtgaagtcg gaacggttgt tttattcggc cctgatcggc
     7441 tatatctggt acgaggcccc agcggaggaa atgaacttct caacgctgct ggaaatgata
     7501 aacgccagtg aagcccgcga ggacgacccg gagtttcaaa gccccgtcga tctcatgttt
     7561 gagcgtttgg agcagaaaga ccccgaccat tttgcggtgc gccagtacaa gaaattcctg
     7621 ctgtccgcag gcaagacccg ttcttccatc ctgatttcat gcggtgccag acttgcgccc
     7681 tttgacatcc gggaggtacg cgagctgatg gaggacgacg aaatggagct ggacaccatc
     7741 ggggacgaaa agacggtgct gtatctcatt atgagcgata cggacacgac ctttaatttc
     7801 attctggcta tgctccaaag ccagctaatc aacctgcttt gtgaccgggc tgatgatgtg
     7861 tacggcgggc ggctgcctgt ccatgtacgc ctgattttgg atgaatttgc caacattggc
     7921 caaataccca atttcgataa actcattgcc accattcgaa gccgtgaaat atcggcctct
     7981 attattttgc agagccagtc gcagctaaaa gcgatttaca aggacgccgc cgagatcatt
     8041 tcggataact gcgatagtgt gcttttcctt agtgggcgcg gcaaaaatgc caaggagatt
     8101 tccgatgcgc tggggaaaga aacgattgac agtttcaaca ccagcgagaa ccggggaagc
     8161 caaacgtccc acggattgaa ctatcaaaaa ttaggaaagg agttgatgtc ccaagacgaa
     8221 attgccgtga tggacggcgg caagtgtatc ttgcagctac ggggtgtgcg cccgttttta
     8281 tcggagaaat tcgatattac caagcatccc cggtacaaat accttgccga cgccgacaag
     8341 aagaatacct ttgacgtgga cagatttttg actatcacgc gccggaaacg gcagcaagtg
     8401 gtaacgcagg atgaagtatt cgacctgtat gaaatcgacc tgtcggacga ggacgccgcc
     8461 gctgaataaa cagcggcttt tttcattcca acaacaaaat caaaaaatca ttatgagccg
     8521 gaaaccggca gaaagaggta aatttatggc attttttaat tccgcagtta ctactttgca
     8581 gactatcgtg atcggactgg gcggcgctct gtgcgtatgg ggcggcatca atctgttgga
     8641 gggatacggg caggacaatc cgggggcaaa atcgcagggt gtcaagcact taatgtcttc
     8701 tatgtgaaaa gattttaaag agaaaatgca catcctacgc agataacatg acaatttcat
     8761 gcaaagcgaa aggagacacc atgaaaatcg aatacacaaa agtgggagac tattatttac
     8821 cgaatcttta ctacccggag gaaaccagac cgattggctt atggggaatg cttcggaagg
     8881 aatatctgaa agaacataaa tctggaactt atacatatct tcttctgacc gccaggctgg
     8941 acagttattt ggcagatctg aatgaacagg cacaggaacg ctttgaattg atcgaaactc
     9001 agatgcgaag tgcggaggga gtttccgagg aactgaagag acagaatcct atggaatggc
     9061 tatgccactg taacaatatc cgcaaccggg ctgctgagat catcaagcag gaactgattt
     9121 atgtataagg gggattctag atatgagaag agaactgatt gaaagttatt atgaagaata
     9181 ccagcgggag ctggaaagat ttcttgaatc tgacccggag atgttcactc tggaagcaga
     9241 aattcatcat tacatagaaa tactggaacc ggtcctcaga gatggcggac aggagatgtg
     9301 gcagacactg gacaggatta tttcagcgaa aaatgcaatg gagggaattg tggcaaaaga
     9361 aatgtatatc cggggatttc tggattatga aagactggtg tgtgaaaaag acagtaagat
     9421 ctgaataatc agaacataga ctatatataa cgaggctttt attgtataga cagtaagagc
     9481 ctcatttaat atagttttca taataaaaaa caggtggtag tacgtatttg ctttgtgtta
     9541 tataagaatt ggtctggctg aacaatggtg aaaatttact atgattttac ttaaatctga
     9601 ttgaaggatg aaaaagtgat gatatcatat agtgggttga tagggttaca gaaaatgtat
     9661 tcacagaggc agagaagaaa taatgaaagt aaaaaatgca gatgaatttt ataaaatatt
     9721 agaaagaaag gctatatatc cggtatttca gccgattgta aatttacaga ctggagatgt
     9781 ggcaggatat gaagctctta gtcgtatcga caggcatgat acgactctta tgatttcaga
     9841 tttgtttgtg attgctgaac aggtaggctg tgtttggaaa ttggaaaaat tgtgcagaaa
     9901 taaagcattg aaggcagcgg cgaataaacc agaacatgca aaattatttc ttaatgtaga
     9961 tggtaatatc attcaggata aatcgttcat acagggattt acaaaccgta aggctgcgaa
    10021 agccggagta ccgtcctgtg acattgtatt tgaaataacg gaacggtcgg atatagaaaa
    10081 ctatcagatt ctgcaacaga ttatgaagca ttatgcagat cagggatatg aaatagcttt
    10141 ggatgatgtt ggagctggat attctggtct gaaccgtgta gtaaatacat cgccaaacta
    10201 cttaaaagtt gatatagaac tggtcagaga tattcaaaaa cataagaaaa aggaaattat
    10261 gatggaattt ctgttgcatt actgtaatga gacaggagca attctgattg cagaagggat
    10321 agaaacagaa gcagagttgg agtgtctgtg tagattaggt gttcattacg gacaaggata
    10381 ttttcttgga agaccggaca gaacgttcaa aaatgtaagg aatgaggcga tgttggtatt
    10441 acgtagactg aataataaat cgtaatgaaa actatatgtc agcaggtgaa aaatgaataa
    10501 gtcaattata acattataaa gatttccatt gatttttaca cctattttac acaagcactc
    10561 acatagattt gatttctgtc ttctataatt gtataggaaa atgtagatct tatataataa
    10621 caggagagga aagagaaatg aacgagaatg tgaaagtggt ttttggtctg atcggtggcc
    10681 ttgccttgtt cctttatgga atgaacagca tgagtgatgc acttcagaaa gccgcaggag
    10741 aacgaatgaa aaaaatcctt ggatttctta caagaaatcc aattatggga gcacttgccg
    10801 gagcacttgt aacagctgta ttgcagagca gttcagcgac gacagttatg gttatcggtt
    10861 ttgtcagtgc gggacttatg agtcttccgc aggcaatttc tgttatcttt ggtgccaaca
    10921 taggaaccac tatgacggca cagctgatgg catttaaaat cagcaattat atttatccaa
    10981 ttatctttgt tggatttatt ctgaactttg ttagcaaaaa agaaaaggtt aaaaatatcg
    11041 gtatggtaat tttttcattc ggacttttat ttgaaggaat tgaaatcatg ggtgaagtca
    11101 tgaaaccttt ggcgggaagt ccggtctttg ttgatctgat gggaaaagtt tcatccattc
    11161 cggttcttgg tgttgtactg ggagcggtca tgacattggt tgtgcagagt agttcagcta
    11221 cgattgcagt tctgcagaat tttgcatcac aggcaggacc ggacggagta agcagtgtaa
    11281 tagggcttac cggtgcgatt ccgattcttc ttggagataa tataggtaca acgatcacag
    11341 cacttcttgc atcaattgga caatctaaaa atgcaaaacg tacagcaatt gcgcatagta
    11401 tttttaatat cagtggaagc tgcgttttta ttttcctggt tccgtggttt gcaaaatttg
    11461 tccagtttat ttcaccaaag ggaaatgaga ttgatgtgat ttcaagacag attgcaaatg
    11521 cacatacaac gtttaatatc gtctgtacac tggtctggct gccactgatt ccgctgatgg
    11581 taaaaattgt tacaacaatc atccgtggaa atgacaaaac agagaaagca gcttttgaac
    11641 ccaaatatct ggatatgaaa gtgattgaac agccggcggc tgctatggtt ctggtatcta
    11701 aggaactgaa ccgacttgga gaactggcag aatctctgtt aagtgattta aaaacggcga
    11761 ttgtagcaga tggagactca aaaactcatg gttcgtttat agaaaatctg gagattgtac
    11821 atcagttaca ggacagtgtg acagaatata ttaccagatt attcgcaagc ggaaacctta
    11881 cagaacaaca gtctgagcag acggcagggt tactgtatgt aaacaatagt attcagagga
    11941 ttgcagacag atgtgaagat attgaccaga tatgtgaaaa agtaaataac aatggaaaaa
    12001 gactgaccag tgaagcctcc tcagaaatgg aagactgcat tgatattatc cagcagcttt
    12061 taaagaaagc aatggaggca atccgtaagg gtgatgctgt agtggctgaa acagttttta
    12121 aaaataagaa aaaaatgcat aaagcagaga aaaaatacag taaggcacat ttaaaccgtg
    12181 taaaaaatga ggtttgtgat gcgtctatga caagatattt ttctgggatc atgtataatt
    12241 ttgaccgtat ggcagataat tgtgtcagca ttgcagaaga agcctgtgat aatgtggctt
    12301 ttataaacct tgatgaagag acgggaaaat caatgatgga aagaggtgca gtataaaatg
    12361 gtaaagaaaa aaaacatccg aaaatctgca cttctgctga taatgataat gctggcaacg
    12421 ggaataacag catgtggaac cgaggaagag ccattgccgg atctgtctgc tatgggagct
    12481 gtcacagctg tatcacgaga agaagggtca ggaaccagag cagaatttga gaatcttttg
    12541 aaactgcctg agagtgatac aggaatagtt gttgattcta cagagaaagt tctgaaaaag
    12601 gtggaagaag ataaaaacgc agttggttat gtcgcatata gttccgcaac agatacgaat
    12661 ggaaaaatac ttcaaatcaa cggagttctt ccttctgaga aaacaataga caataacagt
    12721 tatcctcttt gcagggatta ttatctggct tataatggag aactgacaga tgtggaacag
    12781 gatttcctga cttatgtaaa gagtaaagga caggatattg tgaaacaata ttgtattcag
    12841 gctgacagta caacaacatt cctttctgac aagtcagaag gaaagattct gattgaagga
    12901 tccacttcaa tggaaccaat ggtaaaagca ctggcagatg actatcagaa acaaaatcca
    12961 aatgctgaaa tagaagtaaa agcaacagat tcttcaagag ggattacggc ggtgataagt
    13021 ggagagtgtg atttcgcaat gtcatccaga gaattaaagg attatgaagc agagcttctg
    13081 gaaacaaaag tcattggaaa agatgctata gctattgtga taaatgaaga aaatccactg
    13141 gaaaatctta caataaaaca acttacaggt ctgtacaatg gaacatataa aaactgggat
    13201 gatctttcct gagaaaaacg aatataatat cttacaagca ctgactataa cggtcggtgc
    13261 ttcttttttg gattttttca aaattatctg acattcttca aattcccacg tgtgattagt
    13321 ggaaaaataa ttttttctgc aaaagtaccg tccaaatgcc ctctgaaact tgctattagt
    13381 gagggaatat ttcatttatg cgaaaaaaac ttgcaaaagg gggttggtaa ataccgcttc
    13441 ccaggcttta taggtagagg aacaattatt ttaaaaattt taccccaaag atgtccgctt
    13501 ttgcctgtct cagtacagag tatgtgaagg gactaacaaa agtaaaagaa aaaatgataa
    13561 aaatgcaaaa aaatatcacg aatcttttgc atcttgcgaa gtcatttgac tctagtaatc
    13621 agaggacgtt ttggaatgtt ccttcgatga accttgacaa ccgataagtg caccctagaa
    13681 gcctaacagg agttatgcca tgacctttct ttttctgaga gcgagcgaaa ttataatgac
    13741 actcccgatg atgggcggct gaccgaagcc ttatggtcag tgacctgctg atgcaggcag
    13801 aggcagtctg agacggctgc aggtgacagg gaaacgcacg ccagaccgcc ggacgaatga
    13861 aactgatgac aatgtttaca gggcaaaatg cccggaaagg agcagtttat gacagaactg
    13921 aaaatgaaag aaatgcagtt gattaactgg tttggatgcc cgaatctgat ttatacaagg
    13981 gagagaatcc atctggttgc catgcttaca acagatgaag aaatgaaaaa gaatctttac
    14041 agggtatgca gattccttgc aagagaagaa atcgcatatc ggtatccgat catgtatgaa
    14101 atgatccgga aaatattttc accggatgat accaatccac cggaagcatg tgcaatgttt
    14161 atgccggacg atgaaacaga tgatgaagaa tatgcaatgg cagcgtagct gcggaaagga
    14221 cagtaacgat tatgaataaa gtggttttaa tgggaagact gacaaagaat ccggagatca
    14281 aatatgccgg aaaagataat gatatggcag tagcaagata tacactggcg gtaaacagaa
    14341 gatataaacg tgacggtgaa caggaagcgg attttatttc ctgtgtcaca ttcggaaaga
    14401 gtgcagagtt tgcacagaaa tatcttcaca agggaatgag gattgtgatt ggcggtcgta
    14461 tcagcactgg caattataag gacaaggacg ggaaaacaat ttatactaca gatgtgatcg
    14521 tggaagaaca tgaatttgcc cagaacaagg ataatggtgt tggagctgat tcatcggaaa
    14581 caccgaaaac agataaggac ggatttatgg aagcaccgga aggtgaggtt ccattcgact
    14641 aaaccgaagt aaatggttgg agaggttgcc ctttgtcggg gcatcctctt ttccatttta
    14701 cagaagacat caaaaattac aggtattcaa cctaacgaaa tcgtgatata atcaaaatat
    14761 gttcagatga aaaatatgga ggttgcttat gggcgtaagt tataacagat tatggaaaat
    14821 gctcatagat aaaaatatga agcggataga gatgcagtac ctcaccggga tcagtggaaa
    14881 tattcttgcc aggatgggaa aaaaccagta tgtatcaatg gagacgattg agaagatctg
    14941 caagaagctg gactgcaccg ttgatgagat gctggaattt acagatgatg agtaacaaga
    15001 gcagacatgt caaattatta aaactggaag aatgcttcag aagtcgaaaa aagaaattga
    15061 aaaaagataa cacgccggat acggcataaa tctcttagca acgctaagag cgcacttcta
    15121 tacaggcaat tccatctgcc tgtatcgtgc gacctgcggt gctgttcccg atttcaagaa
    15181 atctcaaacc gggaaataca gcgaaatcag agatttcgta caaggggaag atccccttgc
    15241 ggcacagatg cctatccctg ataaagtttg tgactgaata tttcagatat agacatataa
    15301 ggggaaagaa agtgtgaaat caaaatttgt ggagagattt tatgattaga aaagcaatac
    15361 taaaagatgt taattcgatt gaagatactt acaatgagca tttccagtat gaactgaatc
    15421 atacagcctt tactgtattt aagaaagggg tttatccaac gaaagatgat gcagaacggg
    15481 ctatttatgc tggtgctttg tttgtatatg aagaaaacgg aacaattgtt ggaagtatca
    15541 ttattgataa agttcaacca atagaatatg caactattcc ttggaaagaa aaattatctg
    15601 aagatgaggt aatggtaatt caccttttaa tggtacgtcc gagtatgtct ggtaaaggaa
    15661 ttgcatcatc actaataaaa ttcgctacag aattagcaca aaaaaatagt tgcagggcac
    15721 tacgtttgga tacaggaagt caaaatattc ctgcgctatc tttgtatcaa aaaaatggat
    15781 ttgaaataat tgctagtgct tctaaaaaag ttggagatgt aattgcacac aaaaaccatc
    15841 tttttctcga aaagatattg tagttcgtaa attccagttt gaaaaattga gaaaatcgga
    15901 gtttgaggag catcaccgtt atgattagag aattacggaa agtggatata aataaagttg
    15961 cagaaatatg gttagataca aatataaaga cacattattt tatctctgct caatattgga
    16021 aaagtaattt tgagttagta aaagaacttc tattacaggc aactgtctat gtttacgagg
    16081 ataaacagga aatacagggt tttatagggt taagtaacga atacattgaa ggtatttttg
    16141 tttctgccga aatgcaatct caaggtatag gtaaaatttt gctaaactat gtaaagggaa
    16201 aaagaa
//
LOCUS       DACDPS010000055        15316 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.055, whole genome shotgun sequence.
ACCESSION   DACDPS010000055 DACDPS010000000
VERSION     DACDPS010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.055"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IWJ07_001891"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IWJ07_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP9333467.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="IWJ07_001892"
     CDS             complement(322..891)
                     /locus_tag="IWJ07_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAP9333468.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="IWJ07_001893"
     CDS             1077..1979
                     /locus_tag="IWJ07_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9333469.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IWJ07_001894"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IWJ07_001894"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAP9333470.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IWJ07_001895"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IWJ07_001895"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAP9333471.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IWJ07_001896"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IWJ07_001896"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAP9333472.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="IWJ07_001897"
     CDS             8547..8732
                     /locus_tag="IWJ07_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAP9333473.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="IWJ07_001898"
     CDS             complement(8794..9990)
                     /locus_tag="IWJ07_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9333474.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="IWJ07_001899"
     CDS             complement(10160..11596)
                     /locus_tag="IWJ07_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP9333475.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="IWJ07_001900"
     CDS             11889..12698
                     /locus_tag="IWJ07_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9333476.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            complement(12884..15037)
                     /locus_tag="IWJ07_001901"
     CDS             complement(12884..15037)
                     /locus_tag="IWJ07_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAP9333477.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT         4491 a         3241 c         2683 g         4901 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gacaattttt gtcacaacct tttttgccga gtaaacggtg ttcaaaagcc
    12781 agtttctcgg tatgaagtca aatttaagca aaaatgtgag aagctatttt cacaaaattt
    12841 ttcttcatac tcgaaactaa gcggcttttt cacgacctct tttttatttt gccatgaaca
    12901 tatcataata cgcgtccatt atttctttag caatcgtcaa gtttatgtgg tcttgttcgt
    12961 ctaataagtt cggcaagaca acactgatgg caatttccgg attgtctgtt ggtccgtatg
    13021 ccacgatatt actattgact gtcgcaatat ctggatgacc ttccgcaacc gtttctgcag
    13081 tccctgtttt tgcggaaagg tccattttcg cactggctaa tggtttggct gttgtgtaag
    13141 gatcggtacc atggacaact tggtggaatc cttcacggag gatcgccata ttttctgcag
    13201 aaatattgac cgtattttct actgttgtgc caatttcttt cttcacttca cccaagtttc
    13261 cattttcgtc attgccatag atcgctttga ctaggtgcgg ctgtactcgt ttcccgccat
    13321 tagcgactgt tgaagcatat tgagctaatt ggattggtgt atatgtgtca aactgtccaa
    13381 atgccaaatc ggtaaagtta cctggagcat accattcttt gccattatcc gcattgaact
    13441 tcttcatata atcaacagaa cgtgagatcc caggagattc attcggcaaa tcgatcccag
    13501 tcgttgtacc aagaccaaac tctttgaaag agtctctcaa ttgctgataa gctttagctt
    13561 cttcatctaa actaggtagt cccattccag gtgtatagtc tagtcctaac atctttaacg
    13621 ccactttgat catatacgta ttagaagaca attccgatgc tttgactgca tcaagagcaa
    13681 cttgtccgct tcgattaaag acagagcttt tttctgaagc accttgcaga cgaatcggct
    13741 catcaatcaa aacttgattt ccgctgatcg cgttacttgc ccagcctgct gtcaaagtac
    13801 cagccttgac tacagaccct ggatcaaagg cgctagtaat cgtgcctaaa gcattctcgg
    13861 tgatttcttt ggaaccttgt tcatgactga aaccagtcat tgctagcact tctcctgtct
    13921 gaggattcat agccactgca taagcaccag gcgaatactg tgctttgccg ttgttgatca
    13981 atgtttgata attccttttc aaaatttctt ctacttttga ttggaattct gcattcatag
    14041 ataacattaa attagaacct ttttccccag agaagatttc tttttgattc gaaacatttc
    14101 cttcattatc aagggaaact tcatattggg ttttggttcc ttgcaggaca tcttcatatt
    14161 gtttttccaa ataactttgt cctacacggt cattccgaga ataacctttt gctaggtatt
    14221 tctctgcttc atctgctgga agtccttgct tttcagtcgt cacactgcca agaatacttt
    14281 tcagtgaact tgctgcatta tattctcgtg tccagtctgt gccagtcgaa actcctggaa
    14341 gttcagaagc gcgttctgcc acaacagcta actcttgatc agtaacacca gagtttttga
    14401 tatagacagt gtttaattca tatgcactat tcatccgttt aaagatggta gcaattttta
    14461 attgctcctc gtttaaatta atctcatcag ctgtgacttt attgaccatc gcactatatt
    14521 gttcgctgtt ccctaattgc ttttcttttg ccgaaagtct ttttgtggct tctttcaaat
    14581 gtttgggatc agccagccaa aaatcttttt tatctctatc tgtcagattt tcatctaccg
    14641 gaacatcgat cagcgagttc agcttcgttg cgatttcaag caaatcttta gccgtaactt
    14701 gtgttcctct cgtatatgtg atggcctgat tcgctttatt cgtaactaaa gcttttcctg
    14761 tggagtcgta gatcattccg cgaggagtac tttcttggac cgtaatcgta gaagtagact
    14821 tcactcgcgc ctccatctct tcgccgttca cgatctgcaa ataacccaat ctagcaatca
    14881 acgcaacaaa taacgtaaat ataatgaaaa aaagaaaatt taagcgaaaa ggaacatggg
    14941 accggcgaaa cgttttgttc ttaggttctt gtgcaccttt tcttattttt tctaaccaag
    15001 aagtatttaa tatttttttc ataaaatctt tcatcatttc ctatccttcc gcctatttac
    15061 attcatgctt cattgtaccc aaaaacttct taataaacaa gaagtattta atattttttt
    15121 cataaaatct ttcatcattt cctatccttc cgcctattta cattcatgct tcattgtacc
    15181 caaaaacttc ttaataaaaa agaaaaaatg ggcggagcaa gaaattcttt acctaatttc
    15241 aagataaaat caactttttg tttcacagca atcacttaaa gttacatatc caactatttt
    15301 tttcattaaa aaataa
//
LOCUS       DACDPS010000056        15090 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.056, whole genome shotgun sequence.
ACCESSION   DACDPS010000056 DACDPS010000000
VERSION     DACDPS010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.056"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(13..243)
                     /locus_tag="IWJ07_001902"
     CDS             complement(13..243)
                     /locus_tag="IWJ07_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333478.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            complement(679..1245)
                     /locus_tag="IWJ07_001903"
     CDS             complement(679..1245)
                     /locus_tag="IWJ07_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP9333479.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1245..2201)
                     /locus_tag="IWJ07_001904"
     CDS             complement(1245..2201)
                     /locus_tag="IWJ07_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAP9333480.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2201..2857)
                     /locus_tag="IWJ07_001905"
     CDS             complement(2201..2857)
                     /locus_tag="IWJ07_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP9333481.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIISFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3227..5245
                     /locus_tag="IWJ07_001906"
     CDS             3227..5245
                     /locus_tag="IWJ07_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9333482.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            5672..8086
                     /locus_tag="IWJ07_001907"
     CDS             5672..8086
                     /locus_tag="IWJ07_001907"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAP9333483.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8434..9987
                     /locus_tag="IWJ07_001908"
     CDS             8434..9987
                     /locus_tag="IWJ07_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP9333484.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10368..12152)
                     /locus_tag="IWJ07_001909"
     CDS             complement(10368..12152)
                     /locus_tag="IWJ07_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9333485.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12167..13888)
                     /locus_tag="IWJ07_001910"
     CDS             complement(12167..13888)
                     /locus_tag="IWJ07_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9333486.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13988..14749
                     /locus_tag="IWJ07_001911"
     CDS             13988..14749
                     /locus_tag="IWJ07_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9333487.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4903 a         2771 c         2977 g         4439 t
ORIGIN      
        1 aaagcataac cattactccg atctattttt aaacttagtc tctacctcta atatatatcg
       61 agtttttatt ttgccttcag agaatactgt ttcttttttt gcagttacag gttgtttatt
      121 ttcggaaaaa gctaatatag ctaaaattga aacatccatc tggaatttat cttttttact
      181 gctttgatca taaaagtctg catattcatc actgatattt tttctaataa attcttccat
      241 cataaaaatc acctcaaaac gattatacga acttacgttc ttgttgtaaa gcgatatttg
      301 aggggcaaaa aaggggcata atttgactga tattaagtta gctattgata tatgaatgac
      361 atacaaaaat ataaaatctt ttaaaaatgc ttatatatca atgtttttgc cattctttta
      421 tatatctgtt atatgtcaaa aaaatgtgtt caatttcaat ttgtatttga ataaagaggt
      481 tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca atcaaaaaca gcttctcatg
      541 tttttgattt ggttggactt attaccgcag atataagctt ttgagcaccg tgaataaaga
      601 ggttatgaaa tcaacgctct aacctgagta ttagagaaaa aaacgcaaaa catatcattt
      661 atgttttgcg ttttttattt atcttttttt ctcaatacaa tataaaatag gcggctggtt
      721 cttttggttg ataaaccgat aattcagcac attatatgtt tcctgtggca gtttggaaca
      781 aaaagaatca accatatcca gttctttttc tccaccttca tgaccatagt agacgacaag
      841 gatcaatcgt ccgcgaggca caagtcgttt caatatttct tccatagctg tgcgtgtagt
      901 ctctggcata gtgatgattg ctttatcact ttttgggaga taccctagat tgaaaatccc
      961 tgctttgaga ttttgttgtt cagctatcac ttctcctaac gtttcgtggc cttgtagaaa
     1021 caatgagaca cgttcttcta actgacgctg ttctagcctt tcacgagtag catcgattgc
     1081 ttgttgctgg atatcaaaac tatagacatg accagttttt ccgatgtgtt ctgctagaaa
     1141 aaccgtatcg tggccattgc ccatcgttgc atctacgacg tgatcccctg gttgcaagat
     1201 ttcttgcagt aatgtatggc tgaaatgcaa agcagtttgc aacatcagac gctcaccttt
     1261 ttccgtacat catattttcc ttggaagcta tctctgcgaa gaagttcttc atcaattgca
     1321 ttcaagactt cccatttttt caagctccac attggtccaa tcaaggaatc ccacggcgca
     1381 tcccctgtca aacgatggat gatgatctct tttggtatca tttccagctg gtcgcaaatg
     1441 acattgacat agtcaggacg gctcattaat tgaaggcgac cttcatgata gtcccgcaac
     1501 attctcgttt tgcgcatcag atgcatcaaa tggagcttga ttccttggat atctgaatct
     1561 aaaatggtgc gtctgacatt ttctttcatc atttctaaac tttcgcctgg taatccgttg
     1621 atcaaatgcg tacaaacacg gatattatgt ttgcgtaatt tagccacgcc atctaaataa
     1681 gtttgataat catgtgctcg gttgatcagt tttgaagttt cttcaaaagt tgtttgcaat
     1741 ccaagttcta cccataaata catccgctcg ttcagttcag caaggtaatc aactacttca
     1801 tctggcagac aatccggtcg tgtaccgact gataatccta cgacacctgg taaattgact
     1861 acttgctcaa atcgttcttt gattatttca actggcgcat gggtgtttgt aaaattttgg
     1921 aaataaacga tgtattgttg gacatgaggc catttttgat gcatcatgtc gatttctttc
     1981 tggaattgga ttggaagagg gtcttcaggt gcaacgatca tgtctcccga accagataca
     2041 ctgcaaaaag tacaaccgcc atgggcaaca gtcccatctc gattcggaca gtcaaatcct
     2101 ccatcgatcg gcaccttgaa aattttcccg ccaaactcat ttcgtaaagc gtaattccaa
     2161 gaatgatatc ttttatttcc ttctgaataa ggaaaagtca ttaaaagtcc ttctttctgt
     2221 aagagttatc tatttgtttt cttttttctc tatacactgg ataagtcagg cacagccaag
     2281 aaagactgag taagtagccg ccgatgatat cagtaggaaa atgaacgccc agataaatgc
     2341 ggcttatccc gacacttact ataataaaca tcatcagcaa ttgaagcaat gtgcgtaagg
     2401 ttcttttttg aacaaaagct ggaagcagaa atagtaaaga accgtaaaga atcatactgc
     2461 ctgctgcatg tccactaggg aaactgtaac ttgtttcaac tactaagtgc gccaatgaag
     2521 gacgctctcg catgaaaaat agtttgatca ccgggttgat gattccagca attacaataa
     2581 atccagaaac gaaccaaaat gtttctgccc tttttttctt ataccacatc cataaagccg
     2641 cacaagcaaa tgaaatgatg acggtaagag gattaccgaa tttagtcata aagagttggt
     2701 agctgttcag atcaggataa gaagctctga caatgcctgt aaaccactga tcgaaaccag
     2761 aaagccacga cagatggaac tttacgatcc agcccaaaat gaaaaacagg acaagaaaga
     2821 agctaccagc cacctgaaat gatgttttat atttcataca attcctcctt tgaagcaatg
     2881 acttattata gcatttttgg tccagcgaaa aaaacggtaa agagataaaa ccaatgtatg
     2941 attcctcttt gaataaccaa tattcttata aaaaaaataa ctgtaatcgg aaagacattt
     3001 tttggtaatg caaatgaaat gttagggaaa taaaatcagt gtttttcgaa atccttttaa
     3061 acaagaacgt agacgtagga agaacttgaa aatgaacaga agttgttcat tacggtaaat
     3121 tacaatatga ttacaaaaaa caacaaagct cttaaataac ttttaataaa tattaagaga
     3181 agttataatt attaataata gataaattga agttggagga tagaagatgg aaaacattgc
     3241 aagaaaagaa cggagacgtt tagaacagac caaacgtttt cgaaaagcaa aacgaggggc
     3301 agcaatcgtg ggaactgcga tggtaggctg ttctgtcgct gccccattag tacagccggt
     3361 tcctgttcaa gcggatgaaa cacctgttca gttcggcgca agaatcaata cagcggcatt
     3421 tatcacagaa attgctactt atgctcaacc aatcgctcaa gccaacgatc tatatgcatc
     3481 agtgatgatc gcacaagcag ttgtagaaag cggttggggc agtagtgcgt tatctcaagc
     3541 accttattat aatctttttg ggataaaagg cagctatcaa ggacaaaccg tctacatgga
     3601 tacgcttgaa tttttaaata atcaatgggt aaataaaaag gagcctttcc gacaatatcc
     3661 ttcttttgct gaatctttca atgacaatgc ctatgtactt agaaatactt catttggcaa
     3721 cggttattac tatgccggta cttggaagag caacacgaaa tcctatacag atgcgacagc
     3781 ttgtttgaca gggcgttatg cgactgatcc tggttacgca ggcaaattaa ataatatcat
     3841 cgcaacttac ggattgaccc aatatgatac accagctagc ggaaacgcag gcggtggagc
     3901 gactacaggt ggcaataata ctggaagtgc aggtagtact ggcggttcaa gcaattctgg
     3961 taatacaaac tcaggttcag caacttcgac cacttatact gtccaatctg gtgattctgt
     4021 ttggggaatc tcaaataaat tcggtatcac aatggatcaa ttgatccaat ggaacaacat
     4081 ccaaaacaat ttcatttatc caggacaaaa actgacaatc aaaggtagcc aggaaaatgg
     4141 ctcttcgacg aataattccg gtaacaatac caactcttca ggaaatgctg gtacatcaaa
     4201 cggaggacaa acaactggtg cgaaatacac tgttcaatct ggtgattctg tttggaagat
     4261 ctcaaatgat cacggaatta cgatggctca attgattgaa tggaacaata tcaagaacaa
     4321 tttcgtttat ccaggacaac agctaatcgt atcaaatggc ggttcttcta atgctggagc
     4381 aaatactggc tcaaatactg ggaatacaag tggctctagt tcgtccagct caagttcttc
     4441 taatacagct tcaggaacga aatatacagt taaagccgga gactctgttt ggagcgtctc
     4501 aaataaatac ggaattacga tggctcagct aatccaatgg aacaatatcc aaaataactt
     4561 tatctaccca ggacaacaac tgactgtttc aaacagtgga tcaacaaaca gcacgtcagg
     4621 taatacaggt tcgtcaacca atacaaataa cagcagttca gttaatactg gcagcagttc
     4681 gtctcaaggc acttataccg tgaaagcagg tgaatctgtt tggagcgtgg caaataaaaa
     4741 cggcatcacg atggatcagt taatcgaatg gaataatatc aaaaacaact tcatttatcc
     4801 aggacagcaa ttgatcgtta aaggtggaac ttcggcaaat acaaatacag gttcaactac
     4861 ttctgcagcg aaaccaaata caccgaatac gtctgctacg acaagtacat catccactgg
     4921 taatacgatg tataccgtga aagcgggcga gtcagtttgg agcgtagcga acaaacatca
     4981 tatcacgatg gatcagttaa tcgaatggaa taatatcaaa aataatttca tctatccagg
     5041 acagcaagtg attgtgaaga aaggttcatc tcaatcaaca aaccaacaat cagttactgg
     5101 ttcgaaaacc tatactgtga aatctggaga atctgtttgg agtgttgctg acagtcatgg
     5161 aattacaatg gctcaactaa tcgaatggaa caatattaag aacaacttta tttatccagg
     5221 tcaaacgttg attgtagcta aataaaatag ctgttcgaag aaataacgat tgaattccac
     5281 aaaaatattt gttactataa ggctacttaa atatgacaac acttgaaaag gaagtagtaa
     5341 aaagactgaa gtttcagaga gcgcatggct ggtgagaatg tgcacggatg cttttgaact
     5401 cgcctttgag ttgcccttgc tgaatacccg agtaagcaag cggcggtgac ggccgttaca
     5461 acgttcgagg ccttttcact aaggtaaaat taggtggtac cgcgtagatt acgtcctata
     5521 aaaagagcaa tctttttata ggattttttt atttcttttg tgaagtttga gaaaacaaag
     5581 caaaaggaaa taaaaataaa cagattctgc cggttttttc gttatattta taatagaaac
     5641 aagtgttttc aatagaacag gaggacagca aatgagttac aatcataaag aaattgagaa
     5701 aaaatggcaa aaatactggg caaaaacgaa tactttcaac acccatgatg atcctgaaaa
     5761 gccaaaattt tacgcactgg atatgtttcc ctatccatcc ggtcaagggc tacatgtagg
     5821 gcatcctgaa ggttatacgg caacggatat cctttcacgt gtgaaaagaa gtcaaggata
     5881 caacgtgctg catccaatgg ggtgggatgc attcggtctg cctgctgaac aatatgcgct
     5941 agatacagga aacgatccag ctgagtttac ccaaaaaaat atcgaaacat tccgacgcca
     6001 aatcaattct ttaggattta gttatgactg gaatcgcgaa gtgaatacaa ctgatccgga
     6061 gtattataaa tggacacaat ggatctttac gaagctttat gaaaaaggtc tagcttatga
     6121 agcagaagta gcggtaaact gggtacctga attaggaaca gttatttcaa atgaagaagt
     6181 gatcgatggg aaaagtgaac gtggtggcta tgatgtagtc agaaaaccaa tgcgccaatg
     6241 gatgctgaaa atcactgctt atgctgatcg cttattggat gatctagaat tagttgattg
     6301 gccagaaagc attaaagaaa tgcaaagaaa ttggatcggt cgttcagtag gagcaaatat
     6361 tgaatttaaa gtggctggta cggataaatc ctataccgta tttacgacac gaccagatac
     6421 tctattcggt gcaacttact cggttcttgc acctgaacta gatcttgtta gagaaattac
     6481 gacccctgaa caaaaagctg ctgtagaagc ttatatcgaa gagacagcta aaaaatcaga
     6541 tctaaagaga acggatcttg caaaagaaaa aacaggagta tttactggtg cttatgcaat
     6601 caatccagtt aatggcaagg aaatcccaat ctggatcgcc gattatgtat tatcttcata
     6661 cggcacagga gccatcatgg ctgttccagc acatgatgaa cgtgactttg aattcgctca
     6721 aactttcgga ctagaaattc ttccagttat cgaaggtgga gatgttgaaa aagctgctta
     6781 cacagaagat ggtacgcaca tcaactctga atttctagac ggcatgaaca aacaagaagc
     6841 aatcgataaa atgaatgaat ggctagaaga aaatggcgta gggaaaaaag aagtcagcta
     6901 tcgcttacgt gactggctat tctcccgtca acgttactgg ggtgaaccaa tcccaatcat
     6961 ccattgggag gacggaacag tcacagctgt cccagaagaa gagctaccat tacggttgcc
     7021 taagacaaaa aacatcaagc caagcggtac tggagaatct ccattagcaa atatcgagga
     7081 gtgggtcaat gtcgttgatc ctgtaacagg taaaaaaggc cgtcgcgaaa cgaatacaat
     7141 gccacaatgg gcaggaagct catggtatta cctgcgttat atcgaccctc ataacaaaaa
     7201 agaactagca gattatgaaa agttgaagcg ttggctacca gttgacatct acattggtgg
     7261 agcagaacat gcggtacttc atttactata tgcacgattc tggcataaat tcttatacga
     7321 tatcggtgtt gttcctacaa aagaaccatt ccaaaaacta tacaatcaag gaatgatctt
     7381 aggcgaaaac aacgaaaaaa tgtcaaaatc tcgtggtaat gtagtcaatc ctgatgatgt
     7441 ggtcgaaact tacggtgcag atacactgcg catgtacgaa atgttcatgg ggccattaga
     7501 tgcttcgatt gcttggagcg aaaatggctt agaaggaagc cgtaaattct tggatcgtgt
     7561 atggcgtttg atcgttgatg agaacaataa aatgcgtgac cgtatcacta cattgaatga
     7621 tggcaagctt gataaagtgt atcatcaaac agtgaaaaaa gtgacagaag actatgaaaa
     7681 tcttcatttc aatacagcaa tctcccaatt gatggtcttt atcaatgaag cctataaagt
     7741 ggacgctctg ccttatgaat atatcgaagg atttgttcaa ctactcgcac cgattgctcc
     7801 tcacatgggt gaagaactat gggcaatcct tggaaatgat ggtggtattt cttacgcacc
     7861 atggccaaca tacgatgaag cagcacttgt tgaagacgaa gtagaagttg ttttccaagt
     7921 aaacggtaaa gttcgtgcga aaagcaatgt tccccgagac ttaggaaaag atgagctaga
     7981 aaaagcagca ttagccaatg aaatagtgca agaatatatt gaaggcaaaa cagtcagaaa
     8041 agttattgcg gtaccaaaca agttagtcaa tatcgttgct aattaattgg ctctttgtca
     8101 ataaggactg atgagttgtg caaaatcaaa tctgggccag aatgaacttc attctgtctc
     8161 agattttttg ttatttcaaa ttgattaatt gattgatcaa aaaggttttg attttcatgt
     8221 tctcaaacac gttcaatgtc tttttcttct gaatggtctt gtaagagatc tctgtaatca
     8281 tcaaattttt gtctattaat cttagtattt tttgatgtat aaactacctg aaattgagag
     8341 tacataccaa attcataaat tttttattag tgggactagt gattgtatgg attttgacgc
     8401 ataatgaatg aagtatgaga aaaggagaga caaatggatc aagaactatt tattatcgaa
     8461 gtattatcag ctaaacaaaa aattatcgag aaagcgaaaa aaagagcgaa aagaataatg
     8521 ttcaagcttt taagcttagt atttttctct ggtttgcttt ttttcggagc tgtatcgttc
     8581 ctatatcgcc atatgagtgc agtagtccgt tcagctgaag ggatgcaaaa attaaataat
     8641 ttgtatcact tttcgtgggg agttggcgga ataacaattg ttgtgcttat tgtttttctt
     8701 ctacttattt ggctccgtta ccatcaattg tcgcgtcatt tcgatcaatg gcttagtcaa
     8761 agagcacaac agtttttaca taataaacca acttctcaag atgatgacta tctttatttt
     8821 gagaagactt cgacgagtca agcttttcgg atacaaaaaa gtgattgcca gttattgctg
     8881 agatcacttg atgatttacc tatctactta ggcgaaagca aatcaaaatt cggaatgaac
     8941 acattccata tttttttact agaaaacgaa cccagatggg aaaaaagcca gtcttcgcct
     9001 ggtggatacc tgacaactaa gatgagtaca ttagtcactg gttttttcct cattctttta
     9061 gctggaacaa tctattatgg gagatacact cagccaaaaa tgtcttctca tataaatgct
     9121 gcttctcaca tgacaatgaa ctcaactaac gatacgcata tacaaaagac aaatgaaggg
     9181 ttgttagctg atcctaagca aaaggtaaat ttgacaacac aaaagataaa tgatctcaaa
     9241 cttgatacag agacgcactc tctctatatg acaacagata gtggtgctaa ttgggtattt
     9301 gtgccgattg attcgcaatg gttgcgtttt ggcgaataca ccctgacaac ggggctgatt
     9361 cctgaaggtt attggatgga caacacatat gatatttccg ccgatttctc atggtttatc
     9421 tattcttctg gggatgatgt acgtctgctg acttcaagag ataatggtaa aacatggcaa
     9481 acgtctatta tcacccaatc agttcagccg atacgttatc gaaaagcggt attttatggc
     9541 aacgatggtc tatttatctg tacatcagcc agtgggatgt ctgcagaaac tttgtacatt
     9601 tacactacta tggatggcgg aatcacttgg ataaaaagtg gaagtacaat gatcaatcag
     9661 ccagtacaaa atgtcagctt cgttacaact tctttaggtt ttgtttcgac acgtgaaagt
     9721 ttgttttata caaacaacgg aggacgctca ttcaaagaat cagttctgtc gattccggaa
     9781 aactatcaga caggggggat agatcttttt caatcaccaa atgaagttac tcaaataagc
     9841 gcgaatacgt tagagacaaa attttatttg ttgaaaaaag gaccaatcga tgcagggaaa
     9901 atgtttgcct gcctcttccg ttcaactgat aacggggaaa catggcaatt cgttcagcaa
     9961 ctatcgcaag tagaacaagg agattaaaga agagagattg tgagcctgac gttcagcacc
    10021 gagtcacaag aaacaaattc aaaaaacagc ttctcatatt tgttgaatag tgtaactcgt
    10081 gattcggagt tgacaagagc aacaagaagc gattttgaag tatagcttct cgtattttac
    10141 aagatcgtgt cttattgccg ataggttgtc tcttgaacac cgtttattcg taaaaaggga
    10201 ttgtgacaag tcttttgtca ctacttaccg aacgaaactg aaggagctat ccaaaaagta
    10261 tcattagtat tattctttgt gtttctagtg gcaccactag tacatttaaa aaagctatcg
    10321 atcatggact aatcatacat gatcgatagc tttttagata atgtttgtta cgctatctga
    10381 aattgactat tataaagact ggcataaagc gttggtgatt ggagtagttc agaatgtgtt
    10441 cctttttcga cgatatcgcc attcttcatt acaaggatca tgtctgcatt ttcaatcgtt
    10501 gacaaacggt gtgcaatcac aaaacttgtc ctatcttttg tcacagcttc cattgcttgc
    10561 tgaatcaatg cttctgttcg tgtatctacg ctagaagttg cttcatctaa aataacgaca
    10621 ggaggattcg ctagaatgac tcgagcaatt gtcaataatt gttgctgtcc ttgagataac
    10681 gtgccgtttt cgctagaaat gatcgtatca tatccgtctg gcagtgttcg aataaagtga
    10741 tcacattgtg cgatttcagc cgcttcgata atttgttctc ttgtggcatc cattttcccg
    10801 taggcgatat tatcagctac cgttccttca aacagccagg tattttgtaa aaccatccca
    10861 aaagaacgac gtagttctcc acgtgaaagc ttggtaatat caagaccgtc gaattcgatc
    10921 tttcctttat tcagttcata aaaacgcatc agcagattga ctaaagtcgt ttttcctgca
    10981 ccagttggac caacgatcgc taccgtttgt tttggcttta ctgaaaaatc gacttgattc
    11041 atcaatattt tatctgatgt gtaaccaaac tgtacatttt tgaaagctat ttttcctttc
    11101 ggttgttcta aaagtcttgc atctggaagg tctggaattt catctgtttc atctaatatc
    11161 tcaaatatcc tatcgattgc tgccattgcg gattggatcg agttgatcac ataagaagcg
    11221 gtagaaattg gttcagaaat ctgattgatg tactgaagat aagcttgtaa gaagccgatc
    11281 gtcacaccac ctgatagaac aagcatagca ccgaataccg cacttatgat gaaggcgagt
    11341 tgattaatga aacgaatggc cggatagata gcacagttca taaattgagc acgtttaaaa
    11401 gcgttgtatt gctttttatt tacttcgtgt atagcagctt ctgcatgagc ttgctgattg
    11461 aatgttttga ccactagatt accagctaag atttcttcca ttcgattatt taaaacaccc
    11521 agttcatact gattctgttc agaaatctgt ttgtttttgt tcgcaatctt agttgtaaca
    11581 attgaactgc caacgatcag caataagacg agcaaagtaa gcttgctttc gatataaaac
    11641 agcatcagac cagcagcagc tattgttacg acagaagtaa aaaattgatt gatacctgtc
    11701 aataacactt gagaaagttg attgagaccg gtagttgtcc gactaatgat gtcacctact
    11761 tgatgattgt caaaaaacga catcggtaat gttttgaatt tttttgtgat ttcttttcgc
    11821 accctcaatg tgatcttttc gcttaacgct gccattgtcc gttcctgaat aaatgaagtg
    11881 atgctgctta agactgaaca gccgatcagc aataagacag ggaacaacaa tgcttgtttg
    11941 atatcactga aagaaagctg tccaatacct gtttttctta tttctgataa gagatcatcg
    12001 atcccgattc ccatgatcaa gggcatggct acgactaata ggttcccgaa taaactgcat
    12061 agaaacaatc cgtaaaaata tttatgttca ggtttgatca ttttccaaaa acgtatcaaa
    12121 gcacgcgtgt tcaacgtttt tttcttttcc atcgtatccc ttctttctat attccttgtg
    12181 attttgcaaa ctcctgataa gtttggcaat tagacagtag ttcttcatga gtaccttgtc
    12241 caacgatttt tccttcatct aaaacaatga tgttatctgc ttgttggatt gtactgaggc
    12301 gttgagcaac aatcacgaga gttttctccg acatgtgttt atgaagggct gctcttaatg
    12361 ctgcatctgt tttatagtct aaagcggaga aactatcatc gaatacatag atctctgctg
    12421 gtttgatcaa cgctcgagca atacatatcc gctgtttctg gccccctgaa taattagaac
    12481 cgccttgagc aacaaagctg tccaatccgt caggaagggt gtccactagt tcttctaatt
    12541 gagcgatctt gatcgcttta gacaactctt ctgttgaggc atattcattt cccatctgca
    12601 agttgcttaa aattgtcccg ctgaataaga aagccttctg agggacataa ctgatgtggg
    12661 agcggatctg ttcttgagtc atttgagtaa gaggaatacc accaagggtt acttttccta
    12721 tcgtagggtc actaagtctc aagagaagtt tcgccaaact actttttcca gatccggtcc
    12781 cgccaacgat cgctgttgtt tttcctttag gtattgtgaa atcaatatct tctagaactg
    12841 gatcagagat ttcagtatat ttaaatgtga catgttcaaa agcagctgca tttgtctgat
    12901 caaaggttgg ttcagctact gttttgatcg tctcttcctc aatttcgatc tctgcttgca
    12961 gtatttcttc tatccgtcgt aatgaggcaa acgatttagg gataatgacc aataccatag
    13021 ctgcgatcat caagtaagca agtgtcagca tagagtattc gactacagca gtcacgacac
    13081 cgatttgtaa tgctccttct gctacaagat ttccgccaaa ccacaaaata gaagcataaa
    13141 cgattcccat gagcaaaaaa gcaattggag tgacccaagc aaatattcgg ttcactttga
    13201 tcatttgatt agcatagtca gcaaagctct tattcgttcg cttttcttca tatgattgat
    13261 tatcaaaggc acggatcata ttaatcccag taaagaattc tctcaatgtg atggtgatcc
    13321 gatccatttt tggctggata ctcaaagata aaggtgcccc tcgtttcatc agtcgataaa
    13381 caatccatac ataaaaaaca acagagatca atgtaaccca tgttagtaat agagaataac
    13441 gaaacatcat gagaatggtg aaaacgcaaa taaccggtgc tggtaagatc atttggcaaa
    13501 ataaaacaat catttgctgg acattatcga catcattcgt cattcttgtc aaaagagatc
    13561 cagaactaaa acgactgata tccttcattg acagctgttg gaatttacgg aaatatttct
    13621 cccgcgtttg gaatccaaac ctagcggaag ccaatgctga aaagtaacta gaggcaactg
    13681 cagcgattgt tcctagaaac gctacaagca gcatttcaac tccaattttt tttataactg
    13741 aggcttcgcc acttgctata cctgtgtcga tcagctttgc tactaatttt ggtacaccta
    13801 atgtaccggc gatttctaaa cagatcatca caaaggtaag gagagcgagt cctttgtttt
    13861 tctgtaagaa tgtttttaag atagacatat tattcctcct tttttcctga tacttttcga
    13921 ttctatacct tcaagtaact tgaaggtcaa tagaaaaaag aataaactga aaaagaggtg
    13981 agaaaaaatg aaaaaaatgt atacgattgg ccaagtatcg gaactatttg atttgccaaa
    14041 atcaacgatc cgctattggg atgaacaagg attaatccgt tcaagtagac aagaagagaa
    14101 tgattatcga ttgtttgata tcgatgatat ttttatgctt tatgatatcg ttttttatag
    14161 aaaattagat ataccgatca aacagatgaa gaatttatat ggaaaaacat tgacggagtt
    14221 atatgagaca cttgatgaaa cagaaagaag aatccataaa gaactggtgg tgatgaaaca
    14281 aaaacaaaaa gagataaggg agcaaaaaaa acagttgaaa ttgatgatcg atacaaacga
    14341 ggaagaattt cctgtagaag agatcccttt tgactgtatg atctcaacgg agtttgaaga
    14401 tattgtggaa atcaaaaaat ttctacctaa ctattcaagc tttgggatga tgagtatgcc
    14461 agcttccagt tcacagacga tgtatggatt ttttatcgac cctagtgaag tacatttatt
    14521 tactcaagac gtgatttggg agaaaaaaga tacggctgtt tatcgtcgat tcttattgaa
    14581 atccgagatg aaccatgcgg aacgtaataa tattttagaa atcagagaaa gaatgtatga
    14641 aaaaggatgg aaaacaggag aagtgatcgg ccagtatcta ttgacgaata cggatgaaaa
    14701 caatatcaga actgaatatt atcatgcatg gatagaaatg aaaaaatagg aaatatgtgt
    14761 ttaagataac tcgaaaatga tggttgtttt agttataaag aaaaattttc tggttcgatt
    14821 acgggtttaa tgtgggggat tattagtcgt ggatagatag aattttagtt ttttagagga
    14881 aatatgtctt taaaaatgga atggttgcaa ttcttagcga tatatacgat gtaattgtcc
    14941 tcttaaaaaa gtatggaaaa ataattgtat tgggtgttat gtataattca tggtgaactg
    15001 agactaatga gaagaaaaat tcatattgtt atgagtgaga ttgtatcaga tgagaatgat
    15061 aaattaaata ggtgcataaa agcattacac
//
LOCUS       DACDPS010000057        14832 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.057, whole genome shotgun sequence.
ACCESSION   DACDPS010000057 DACDPS010000000
VERSION     DACDPS010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.057"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(16..729)
                     /locus_tag="IWJ07_001912"
     CDS             complement(16..729)
                     /locus_tag="IWJ07_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9333488.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            904..2328
                     /locus_tag="IWJ07_001913"
     CDS             904..2328
                     /locus_tag="IWJ07_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9333489.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            2627..3334
                     /locus_tag="IWJ07_001914"
     CDS             2627..3334
                     /locus_tag="IWJ07_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAP9333490.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            3321..3644
                     /locus_tag="IWJ07_001915"
     CDS             3321..3644
                     /locus_tag="IWJ07_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP9333491.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(3733..4770)
                     /locus_tag="IWJ07_001916"
     CDS             complement(3733..4770)
                     /locus_tag="IWJ07_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9333492.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(5091..6074)
                     /locus_tag="IWJ07_001917"
     CDS             complement(5091..6074)
                     /locus_tag="IWJ07_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAP9333493.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(6489..7259)
                     /locus_tag="IWJ07_001918"
     CDS             complement(6489..7259)
                     /locus_tag="IWJ07_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9333494.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(7252..8187)
                     /locus_tag="IWJ07_001919"
     CDS             complement(7252..8187)
                     /locus_tag="IWJ07_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9333495.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(8503..10152)
                     /locus_tag="IWJ07_001920"
     CDS             complement(8503..10152)
                     /locus_tag="IWJ07_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP9333496.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(10269..12872)
                     /locus_tag="IWJ07_001921"
     CDS             complement(10269..12872)
                     /locus_tag="IWJ07_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP9333497.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLILIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(12884..13933)
                     /locus_tag="IWJ07_001922"
     CDS             complement(12884..13933)
                     /locus_tag="IWJ07_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAP9333498.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(13959..14273)
                     /locus_tag="IWJ07_001923"
     CDS             complement(13959..14273)
                     /locus_tag="IWJ07_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAP9333499.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(14289..14735)
                     /locus_tag="IWJ07_001924"
                     /pseudo
     CDS             complement(14289..14735)
                     /locus_tag="IWJ07_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
BASE COUNT         4393 a         3072 c         2495 g         4872 t
ORIGIN      
        1 ataattttcc cgagcttatt tttcaacaaa aacagcttca aaaacaaagt cttgatataa
       61 atgtctcgat accgaatact cgaaaggttt accagacgat agagcaacaa ctttgtctat
      121 ctcaatcata aattcacctg gatctatttt taatattttt gcgtcaaatt cattcgcagt
      181 atctcctttg atacgtatgt gcgaagtctt tatttccaga cccagttcat ttgtgatata
      241 agcatataca gatttcttta aatgttttgg ttccaagcct ggtatgactg ctaatggcat
      301 gaatgtttgc tcaatcgagt atggttgaca atcaataaaa cggcttcgta caatattata
      361 tacatagtct tttacactga tacttaattt atcagctact tttttcgatg gaagttcaat
      421 tgagaaagta tggatttctg aatctacgtg ttcttcacca accacgctaa acaaaccttc
      481 gtttggacca agaactcgtg agttatcaat tggtgttctg acgacttcag tcccataccc
      541 acttctactt cgcaaaactc cttcggaaac aagtaagtct acgcctttct ttactgttaa
      601 cttgcttgta tgataaataa ctgccaattc atctcctgta ggaagtttct caccaatttt
      661 atactctcca tttacaattt ttttctttat atctaagtaa atttccatat acttcgcaat
      721 ttttttcaat ggtgaccctc caatcaaaaa catatcttca tctatacttt aacataaata
      781 aaccgataaa atccacatca aaattataca cacaataaaa aagatatata tatttaaaca
      841 aaactattta ctttaagttg taagcggtgt tatcattaag tggaaacagg aggtaaaaaa
      901 attatgacaa ctatgaaaaa gctaactttt ccagatgatt tttggtgggg ttccgcttgg
      961 tcagctgagc aagcagaagg aaaaggagaa acaggaaaag cagagactat ctgggatcgc
     1021 tggtttaaag aacaacctta ccggttttat caacgaatcg attcctctat tacaaccgat
     1081 cacatccatc aatataaaga agatatagaa ctaatgaaaa aaactgggca taactccttt
     1141 cgcgtttcta tttcttgggc acgaatgttt ccaaatggcg gtaccggaaa agtaaatccc
     1201 aaagccattt ctttctatcg aaaattattt tccgaaatga ataataacgg aatcaaagta
     1261 tttgccaact tatatcattt tgacatgcct gctactcttc aagataaagg aggatgggaa
     1321 tcacgtgaag tagttgatgc ttatgttcat tacgctgaaa cttgttttaa agaatttggt
     1381 gacttagttt gtcattggtt taccttcaat gaaccacttg ggcctatttt aggtacttat
     1441 cttgaagatt ttcattatcc aaacctcatt gactttaaac gcggagcgca agcagcattc
     1501 ttcactatac ttgcacacgc aaaagcaata caagtattta aagaactagc tcttcctagt
     1561 aaaataggcg ttattctaaa tttgagtcct acttttcctc gtagtcaaaa tcctgctgat
     1621 gtgaaagcag cagaaatcgc tgatttattt tatacgcgga gctttttaga tcctatggtt
     1681 aaagggaatt tttctaaaaa gcttatcctg ttattacaag aatataatca gcttcctgaa
     1741 gattatacga aagaagatct tgtatgtatc tacgaaaata ccgctcaaat tcttggacta
     1801 aactattacg agccacgtcg cgtcaaatct cgttcttcta tggtggatac atctagtcca
     1861 tttttaccag agtggttttt cgaaccacat atcatgcctg gacgacgaat gaacgaatac
     1921 cggggatggg aaatttacga aaaaggaatt tatgatttgt gcatggatat caagaaccat
     1981 tatggaaaca tcgaagcatt tatctcggag aacggaatgg gcgttgcgga tgaagaacgt
     2041 ttcatggatg atacagggca aataattgat gattatcgga ttgagtatat caaagatcat
     2101 cttgcttact tatggaaggc aataaatgaa ggatgtaaca ttaaggggta tcatctttgg
     2161 acatttattg attgctggtc atggatcaac gcctataaaa accgatatgg gctggtttca
     2221 ctcgacctta ctactcaaaa aagaacaatt aaaaaaagtg gcgaatttta caagcaatta
     2281 agtgatacaa atggatttag atacgatgta gacttgttag ttgaataata ttctaaaatt
     2341 ccacttgaag tgaacacatt agaaaacaag aataatgaga aataccaata acaaatactt
     2401 tttcttcata cttcatttat acttgtcagt ataagagact gtgacaaatg tagtgatcct
     2461 cagaagttag gtctggaaat ctaacttgag aagatttttt taaactacgt ccagtctttt
     2521 ttatttgttt aaaattgaag ttgacaagtc ttcattaaaa atttagaata ttcacaagtt
     2581 tgacgacaat tatgaaaatt tatgtcgtgc taaaggagtt ttgttattgg atgaaaagtt
     2641 agatataaaa actgcgatca aagacacgtt acctaccgtt tttggttata tcggtattgg
     2701 acttgcattt ggtatcgttg ggaaagctgc cgggtttcat ccgctggtcg tcacgttgat
     2761 gtccctgctg atctatgctg gttctgccca atttatcacg gtcagcatgc ttgctagcca
     2821 tagtccactg ctctccatcg ttctctcgac ctttctagtc aattcccgaa tgattctgat
     2881 gagtatgacg attgctcctt atttcaagaa aaatcggctg cttcaaaatc tgctgattgg
     2941 cacattgctg acagatgaaa gtttcgcact gggaatgaat aaattgaact atactggaca
     3001 aaagttaaac tttcgttgga tgaatacagc taattggatt tcttatttga cgtgggtggg
     3061 ttcttctctt gttggcgcac ttttagggaa ttttattact gatccgaaaa agtttggttt
     3121 ggatttcgcc atcgtcgcta tgtttatcgg gttactttat cttcaagtca tttctgatag
     3181 aaacacctct aaacgattac agttgatcct gattggtctc actttgattc tggtatatgt
     3241 cggactgatt tttatcccaa gtaacttagt gattttagtt gtaacactga ttgggtgcgg
     3301 attgggggtg tggatcaaac atgccttctt ttgagtttac attgctgact attttaggtt
     3361 gtactttagc aacttggctt tctaggatcc taccatttgt cttactgaaa aaattcgatt
     3421 tgccgcaacc gttgcttgag tatttaagtt ttgtcccgat cgtcattatg tctgctttat
     3481 ggttcagcag tctatttacc caaaatatcg gacatctgcc gcagatcaat ctcgaaaatg
     3541 ctttggcttc agttcctaca cttttagctg ctattctttc aaaaagctta ttggtgatcg
     3601 tcttggtagg aattctttcc ctttcgttga ttcgcttatt ataaataaaa aaacaagagc
     3661 ttatggcaaa ttactgtcat gcgctcttgt ttttatcaaa gtcaaataag aaaaaaatcg
     3721 gcgtattctt gcttatccgt tgaacggacc actaacaacg acttttccta atatatgtcc
     3781 tgtttctacg tctttggttg cttgtttcaa gttcttcgca ttcatcccat ctgaatagac
     3841 cttgctcaat gtactgcgga taatcccttc atttgctaaa gcagcaatgt gcgctaaagc
     3901 ttctccttgc gtttcgattt catagttata gtctgtttta gcgaacatat actcccagtc
     3961 gaagctgata gattggtttt tccaaacacc tatatctaac ggctctttga tccctacgat
     4021 cgttcccaca tgtccaaatg gtcggacaag gtgtttgatt tgatcaaagt agcgggtaat
     4081 atcgaataat acagcgacat actctacttg atcgattcct aattcacgaa aacttttact
     4141 taagtcgtga tgataatcga ttggatagtc tacacctgtt tttttcagcc attcaaaatt
     4201 cttcggactt gcagtcgcgt agacttctag gccagcccaa tgcgctagtt gattcaaaat
     4261 cgaaccgacg cccccagctc cgttgatcac taagattttt ttcccgcggt tggcattttc
     4321 ttctggagtc agaccaaatt tttcaaataa caattcataa gcggtcaatg aggtcaacgg
     4381 caaggctgcg gcttcttcat ctgaaagatt ttttggtgca agagcgacaa tacgttcgtc
     4441 aaccagttgg tattcttgat tgcttcctgc tcttgtcgtt gtacctgcgt aaaaaacgcg
     4501 atcgccgaca gaaaattttt gcacttcatc acctacagca acaactttcc cgactccatc
     4561 aaaccctaag atacgtaaag catttcttat cccgttatct tggcgaagtt tcgtatcgac
     4621 aggattaaca gatactgcgt tgacttttac cagaagatcc cgtgaactgg gtacaggtat
     4681 ctgttctttt tcatcaataa agctttttgg atcatcgatc gacaaccctt cataaaaacc
     4741 gactaccttc attaattcct tttctgacat acgaattcct ccttagcttc atcaagttgg
     4801 agcatccata cagttttcat ttcaatatat atagtatgct atttgttcca cttttattca
     4861 tcataattat tctcttactt aaagtaaact atttgcactt ttttggttaa aaaagaggtt
     4921 gcgacaagac ttttgtcaca acctctttta aacggtgttc aatcaactga ccctcggtat
     4981 tcgctcaaaa aacgacaaaa tatgaggagc tatttttgtt tggctgttct tattacttga
     5041 tgcaggacag ctttttcacg acctctaatt cacggtgttc aagcaagaaa tcattgttca
     5101 ctgaaaagcg ttggatgttc tttttcaaag gtgtctaaga tctctcgtac ttcatcagta
     5161 gatttcgtat tcatcaactg aactcgcaag tcatttgctc ctggaaatcc tttgatatag
     5221 attttaaaga aacgatgtag tcctgtgatg gaacgcggga tgatttctga ataatgatct
     5281 tgcagatcta gttgcagacg aagtagtccg atcagttctt tttctgtatg ttccctcggt
     5341 tctttttcaa atgcataagg attcttgaaa atcccccggc caatcatgat gccatctacg
     5401 ccatattttt cggccaattc ttctcctgtc tggcgatctg ggatatctcc gttgatcgtg
     5461 atcagtgttt gcggagcgat ttggtcacgt aaagcaatga ttttagggat gacttcccaa
     5521 tgtgcatcta ctttactcat ctctttacgc gtacgcaaat ggacagaaag attggctata
     5581 tcttgatgaa gtaggtgact gatccattct tccatctcag acatttccgt atagccgata
     5641 cgcgtcttga cgctgacagg caaaccgcca gctttagccg cttcaatcaa ttgtgccgct
     5701 acttctgggc gaaggatcaa gccactgcct tttccacgtt ctgctacatt agggactggg
     5761 cagcccatgt tgatatcgat tccttggaag cccatttctg ccatagcaat gctcatttct
     5821 ctaaaaaatt ctggcttgtc tccccaaata tgggcaacca tcggctgttc atcttctgta
     5881 aaggtcaatc ttcctcgtac actgtctttt ccatctggat gacagaaact gtctgagttt
     5941 gtaaactcag taaagaagac atccggtgct gctgcatgct ttacgacatg gcgaaataca
     6001 acatctgtta catcttccat tggtgctaaa acaaaaaaag gctttggcag ctctttccaa
     6061 aaattagtag tcatttattt gttactctcc attctatcaa gcagtgaagt cgcaatcttt
     6121 ctcaaaaaca ttgtcattat actaggaaac aaaaaaagaa gcaaagtgaa cagcttaaaa
     6181 gatgtttctg attgagcaaa aaaagactgt gacaaaaatc ttatcacaat tccttttccg
     6241 gaataaatgg tgttcaaaag cttgcatctg cggtaataag tccaaccaaa cgaaaaatag
     6301 gaggagctat ttttgtttgg ctgttcttat tacttgatgc agaacagctt tttcacaacc
     6361 tctccactaa acggtgttca atcagctgac cctcggtatt agctcaacaa acgacaaaat
     6421 atgaggaact attttcccgt ttgtttctct aatactcagg tcaaaacgct gatttcacaa
     6481 cctcttttct aacttctcaa tcctcttcct ttattgatca aataccaagt aacggcgtac
     6541 aaaacaacga tgaatagcac aagtacgatc atcgatgttg ctactggcac atcgattgtt
     6601 ccaaggaatc cgtaacggaa accggagatc atataaacga tcggattgac tttcgaaaca
     6661 gcttgccaaa atggcggcaa catagagatg gcatagaaaa cgccacctag ataagttaac
     6721 ggctgcaaga caaatgtagg cacgatcgat acatcgtcat aagactgagc aaaaatcccg
     6781 ttgagcagtc ctgctaacga aaacaagatt gccgtcatca ataatgtaat gatgaccatg
     6841 aaccatgaat acacatgaag tggtacaaag aacaaggaaa tgatcgttac gagagagccg
     6901 acgagaatac tgcgacccac tccgccaatc acaaagcccc aaatgatcac atgcgtagga
     6961 acaggcgcta ccaagatttc ttcgatgttt ttctggaatt tttgagagaa gaaggacgaa
     7021 gaaacgttgg catacgaact agtgatagca gacatcataa tcaaaccagg cacgatgaat
     7081 tccatatatg aaaaaccacc catgtcaccg atccgaccac cgatcatttt tccgaaaata
     7141 acaaagtaca aggatgtcgt gatcacaggc ggaaccagtg tttggaccca gatacgcaaa
     7201 tagcggttcg tttccttgat agctaaactt tttaatgcag taaaataaag attaaacatt
     7261 tttctcctcc gtatgattat tttcttctgt gattttcaaa aacagttctt ctaaacgatt
     7321 cgacttattc ctcatagaaa gaactttgat tccttgtctt gtcagctgat cgaagattcc
     7381 gttcacccct tgatttcgtt caacttcgac agcgattgtc tgttcatctt caaatgtaca
     7441 agcgtatcct tcaatcacag gtttggtctc atatggcgct aaatcaaaga taaatgtttc
     7501 aaattgcagt ttggatagaa gctctttcat actagtattc tcgatcagtt cacctgattg
     7561 gatgatcccg atgttgcgac aaagcatttc agcttcttct aaataatgcg tcgtcaaaat
     7621 gatcgttgta ccattttcgt tcaattctcg gagaaactcc cacatctcgc gtctcaattc
     7681 aatatcgact cctgctgttg gttcatccaa gatcagcaaa cgtggttcat gcatcaatgc
     7741 cctagcaatc atcagtcttc gcttcattcc cccggaaagc atacgtgcac ggacatttct
     7801 cttttcccaa agattggact gcttcaaata tttttcgctg cgtttcatcg cttctttgcg
     7861 agagacaccg tagtagcctg cttgattcac gacgatttgc tggaccgttt caaagggatt
     7921 gaaattgaac tcttgcggga ctaatccaat ctgttgcttt gctcggacta gatccgtatc
     7981 cagatcataa ccgaagacat tcacttttcc agatgtttta ttgactaaag aagtaatgat
     8041 tccgatcgtt gtagacttcc cagctccatt tggtcctaat agcgcataga aatctccttc
     8101 tttcacggaa agatcgattc ctcgcaatgc ttcgacccca cttccataaa cctttttcaa
     8161 attatttatt tcaagcgcat ttgccattta tttcctatct ccttatagac atgattcttt
     8221 attgtatcat ggaatacagc agatgaaaaa acaacttggt tccagcgaaa aatcaggttg
     8281 ctgggctcag gaaaatctcc taatgaaaga catcaatcgg acgaagtcag acaacctttt
     8341 cagaaaaaac aaatcgaaga taaaggatta ctattcatat gtgcaacaaa aggagcgtga
     8401 gacaaaagta aagaacactt ttgtctcacg ctctaaacac gtataaacgg cggaagcaaa
     8461 agccaagatt tttgatccaa taaatacttt cgatcacttc gcctatccca atacatgcca
     8521 ctgccgcttt tcatcaaata acgcggcttc cgtttttgcg gtttctacta aagtctctgg
     8581 gtaatccaaa actttcagca aggcaatcgc atttcgggtc cttgaaggtc cttcttttaa
     8641 tttgaaatca aaactgattc cttttccttc tgttacttgt tcttcaaagt ggacattctc
     8701 acaataattc tttaaaatct ctgtcaattc gatatcatgt gtggcgacaa atgctaaaga
     8761 tggatactcc gcaagccacc gaacgactga agcggaagaa gcaatccgtt cgatcgtatt
     8821 tgtccctttt aaaatctcat ctacgaagca gtagcagcgt tctttggaag ccacttggtc
     8881 caacaaacgt ttgatcgatt tgatttctga aacaaaataa ctgtcgcctt caaataaatc
     8941 atcttcgatt gccatggaag tcaacacatg tccaggttgc agggtaaact gttctgcaat
     9001 cgctgtttgg atcgtctgtg ctagaataca gctgatggct atacttttga catacgtaga
     9061 ttttcctgat gcattggaac ccgtgactaa tgtattcttc tgccagttga ccggattcac
     9121 taccgcttct tttaacaatg gatgatagat atctttcgct gtcacaccgc cctcagtaaa
     9181 ctctggtcgg caagtgatgg gcatataagt acggtaattc aatattgcag cggctacttc
     9241 taattctccc agcaattccc acagcttgat ggcttcagtt gaatgctttt ctaatttagt
     9301 gagaacgaaa tgataggaga taaatggcag catgaacatg atgttgacat attcaaaaat
     9361 caactctgct tcactcccac tcttcatacg aaatgaaaaa cctaatttag ggatgtctct
     9421 taatggttta gtttgctgtt tcagctcttc ttgaagtggt gtcggcagtt ttgctagctg
     9481 attggcggct gaaatcgttg aaaccaaata actcatgcta ttcagctctg tttctagtgt
     9541 ttgtttcttc actagataat agaccgtatt gaatactaac ccaccgattg ccaaatagat
     9601 tcctagtact tgtcctaaaa gaagcaaacc caaacccacc agcggaagca agccaagaaa
     9661 aataaataaa cctacgcgtc caatcttttt actttttcca tccgctagat attgttttgc
     9721 aaaattatga tctttttttc ctaaacgagc gaattgatat tggatttttt ctctcagttg
     9781 aggattttcc tgataaaaag caatcagttt ttctaactgc tgatcttcac caaaatcaaa
     9841 gctgcgtaaa cgttgataca acgcttctga acctacacta gaataggtac tgttgattcc
     9901 ttcgaatact tcgaacatat ccaggtcata ccaagtaaga tcatcgattt cgctatccca
     9961 tgaacggaac ttcttctccg tgagccatgc ttccttcaag ctttcttctt tatcaaatct
    10021 cggttgataa ggaatttttt cccatttgct acgaactgtt tcctttaatt tcaaacgtga
    10081 aaaaatgtct aaagcaacta ataaaataac agctgcaata ataattaaaa cgataaattc
    10141 tctttgaccc atatccctct ccgttccttt atttgttagc tttagtatag cactgttgcg
    10201 ctatttgaaa agagggtaaa aaaacacctt tcaaaataaa aggtgttttt ttcttgtata
    10261 ttacatattt atctctcatt tatcaaaatt gacaaataat catagggagg aattgttgtt
    10321 aattcttgtt tattctccag tgcattgaca actgtcccaa gggaaaggat agcctcaggt
    10381 actatttttt cttgttctgt tggattagct aagcgaagaa caaatccttg ttcatgaaaa
    10441 taagaaggat aaatcgatga tacaattaag tcttcaggta attcaatcaa tgaaaattta
    10501 cgaggaacat caagttcatt ttgtaatggc caaatcttgt tatctaacct gtgaataaat
    10561 acattcagtt tctgcttttg ataagaaata gattgttcta accgtttttc tgcctgtttt
    10621 gccactttga actcattaaa tcttccttct tctacattaa atccaaaagt gactttatac
    10681 tcacctatct cttgagctag cggtgtttcc atcatgacat gcccttcatt tgtcgtatct
    10741 cctgaagctc gcccaggtcg ccaagcaaga ttaggttttc caagttcccc tgttgttgaa
    10801 aataaggtta tgattaattg attttctata cgttcatact cttttaaacc atctgcataa
    10861 aacgtaaaat aatgatcatt ttctgccaca gagacactct tatcgaatat ttctaaatta
    10921 acaggtttct ctaccatagt ttctgaccaa ttcggaggaa tgacatcagg ggtattttca
    10981 ataaatccgt tttgaatttg tgcaattgat ttttcagatt tatctaaaac attaattgct
    11041 aagcgcatac gatgcgagta aacagtatta tgaattttga gtgtgccttc aacgatttga
    11101 ctttctccca gactgaacga tatttcataa gatatttctt ccatttcggg tttttcagat
    11161 aaacgatctt ttaaatcttt aggaagttga gcttttccgt aaacaactaa tgtttcttgt
    11221 aaagaatctt tatatacttt agctgtttct aattttagag atagttctgt atctccttct
    11281 aatggtgaat aatcatacgt atcgccatca tttgctgaat cgatcaaatg aacgaaatca
    11341 agatattgtc tatctccaac tatcaaattc aattgcccat ctttaaaaga aagtttatat
    11401 ttattgttac ttatctcagt tttgtctatc tcgattgctg tttctaattt ttcattattt
    11461 ttctcaaatt caacaacagt gtatcctagt gctggcacat ttacatttat ttttatgtca
    11521 agttcataat aagctggttc atcaaaatat tcgaaacctg tagcaatcat ccgataagca
    11581 tttttccgtt ctggaaaata aacttctttt tcaatgatat tatagggact acctttgaag
    11641 tgtattttct ttgaatcagc tactacatgc acgattttcg atccactata attctcagtc
    11701 gttgtgttga aaacaaggac ttcattcgat tttagcttta atgcatcagc catccgtttt
    11761 acaataaggt tttcgattcc gtcagctatc tcattagctt ctttgattcg atggaaaata
    11821 tcttccgcaa cattatctga aacacagcca ccaatactat catgagcctg gttttgcaaa
    11881 atctttttcc ataaccgacg aagtaggcct ttactgatat ggataccatt tctttctgca
    11941 atcagtatga gcggctcaac tcgttttaga attttttgtt ccaatttaaa attttctatt
    12001 ttcatttttg ttcttactga gccaatagaa cgatgaacac gagagtaaac cggctgacga
    12061 agttcacctt tatattcagg taattctttt ttgtttctga taacttctac ataagaagga
    12121 taatctgaaa taatattgtg gaatttactg tatttattca acttctctac gacttgatca
    12181 aaatttaaaa tcattgactt ttgatctaca cccgaaggta ctaaaatatc tttgtttttt
    12241 ccttgcatat gaagcatttc aactgcttca tccaactttt cttttgcaaa ttcaggaata
    12301 tctttaattg cgtctaaggt catcattcca gtcatataac caaatggaaa attcatggca
    12361 taaacaaatt tatctcccaa tcctttccag ataaaataag gagatggaac catcgtttca
    12421 aagtttaatc ctcgccaaca catgaaatta tcgatttcta cttgatgcaa aagtgtaggc
    12481 aaattcgcat tgaacccaaa cgtgtcaggc aaatacccta ccatcattgg atctccatat
    12541 ttaaggatcg tatcactgat tccaatcatt aagtttctta aaattgattc agcatccact
    12601 aataaggcat ctgtttgtgc ataccacggt cccacgaata gttgtcctgt tttcatcagt
    12661 cgttggataa ccttaagctt ttgcggattg atctccaaat actcatcgac gattgaagtt
    12721 tgtccatcta aacaaaagtt tactttaggg ttcctttcca attcatctag aacttcagta
    12781 aagacctgat cagacaatac gatggcatct tcatttgtaa aataccattc tctgtcccag
    12841 tgcgtgtgtt gaattatatg tgcatttacc atatcagatt ctcctacttt ccttcttcta
    12901 ctgaatttac ttgttgttct tttttatact tgatggattt tagtgcattg accattaacg
    12961 ctgtaaccac tcccccaata acaattgata ctaaatataa aggtatattc gtaacaaaag
    13021 gaattgccat aaaggttgaa agcattgttt cttgagtaac accaaaaaat ccatttagac
    13081 cacatgctac tgcagtgccc accacgatgc ttggtattac tcgtaaagga tctttcacgg
    13141 caaacgggat cgtcccttca gtaatcccca caaggcccat aatcatagct gtctttgcat
    13201 tatctcgttc cgattcatca taaagttttt tgtttaaaat tgttgctaaa aacattccaa
    13261 atggcggaat agcaattcct aataacatgg ctgtactagg tccaaagctt ccgcttgaaa
    13321 aagaagcaac cccaaaagca tatgcgattt tattgatagg tcctcccata tcaaacgcca
    13381 tcattcctcc taaaatagcc gacatcaaag cagcatttgc tcctgacatg ccttctagcc
    13441 aattagtcag tcctgttgtc attgcttgaa taggcgtagc tagtacatac cataatatga
    13501 ttccaacaac taatgtagta acaaaaggaa tcagcataat tgattttaga ctaataactt
    13561 gcatcggtac ttttactttt ttcaatgcat tagctaaata gccacaagct aatccagcaa
    13621 ccattcctcc aataaatccc ataccaagat tttgacacat catcccagtg ataaaagctg
    13681 gtgctagacc gggacggtca gcaattgaat atgcgatata ggctgaaatc atcggtacga
    13741 taaactgtaa tcccgtctga ccaatcattc gcaggactcc ccagaatgta tccgtctggt
    13801 cccaaacacc tgtgccacca aaaatgactg caatagaagt tagtaaccct gctgctacga
    13861 caagcgggat catataggaa accccacttt ggaaatgatt tagaatttct cttccttgtt
    13921 tcttgaattc cattagtaac tcccccacga tatatttttt atttgttaat taatgcaatt
    13981 gcttcatcaa tcgtctcttc accatctttt actacctctg aggtccctac tgttaaaatc
    14041 ggtttaccgt caaagcgacc tcgttctgaa acatttgtat ctactgcaaa tatgacaaca
    14101 tctgcctcgg aaacatccga aattgaaagt ttgttttcta tccccatagc cccctgtgtt
    14161 tctactttaa tatctaaccc tttcttcttc gcaaactttt ctaaattagc ttttgccata
    14221 taagtatggg caatccctgc gatacatgct gttactgcta ctatttttgt catatgaaat
    14281 tcctcctttt aaaatgatag agcttttacc aattcttttt tatttctcga atcttttatt
    14341 cttttaacaa actcgtcatc catcaatctt ccagataatt ctgctaaaat acgtaaatga
    14401 ttagtacctt tatcagtgtc ttttattgct aacaaaaaga cgagatcaac tggttgatca
    14461 tctaatgcgc cccattcaac agaccgcttt aacttggcta cagccactgt tgattcaatt
    14521 actgctaggc ttttgccatg aggaatggcg atatttttcc caattcctgt ggtcatatgg
    14581 ttttctctgt caaatacgtc tttttgaaat tatttgtggt cagacacaaa cccattagtc
    14641 tctaacaatt tcgataattt ttcgactaca ctttttttat cagatgcatt taaatctatc
    14701 actatattat ttatattgat tttttcactg atcatttata agccttcttt caatatataa
    14761 actaattttt caaacgacga actcgttttt aaatttgcta ataactcatg attcgaagcg
    14821 atttcattga at
//
LOCUS       DACDPS010000058        14673 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.058, whole genome shotgun sequence.
ACCESSION   DACDPS010000058 DACDPS010000000
VERSION     DACDPS010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.058"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            85..1824
                     /gene="yfcC"
                     /locus_tag="IWJ07_001925"
     CDS             85..1824
                     /gene="yfcC"
                     /locus_tag="IWJ07_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP9333500.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            1981..3165
                     /gene="iadA"
                     /locus_tag="IWJ07_001926"
     CDS             1981..3165
                     /gene="iadA"
                     /locus_tag="IWJ07_001926"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAP9333501.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3276..3479
                     /locus_tag="IWJ07_001927"
     CDS             3276..3479
                     /locus_tag="IWJ07_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP9333502.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3580..4242)
                     /locus_tag="IWJ07_001928"
     CDS             complement(3580..4242)
                     /locus_tag="IWJ07_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9333503.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4255..5757)
                     /locus_tag="IWJ07_001929"
     CDS             complement(4255..5757)
                     /locus_tag="IWJ07_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9333504.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6305..7984
                     /locus_tag="IWJ07_001930"
     CDS             6305..7984
                     /locus_tag="IWJ07_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP9333505.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8111..8590
                     /locus_tag="IWJ07_001931"
     CDS             8111..8590
                     /locus_tag="IWJ07_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAP9333506.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8809..9489
                     /locus_tag="IWJ07_001932"
     CDS             8809..9489
                     /locus_tag="IWJ07_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAP9333507.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLIDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9497..10828
                     /locus_tag="IWJ07_001933"
     CDS             9497..10828
                     /locus_tag="IWJ07_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9333508.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10843..11805
                     /locus_tag="IWJ07_001934"
     CDS             10843..11805
                     /locus_tag="IWJ07_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAP9333509.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11916..12254
                     /locus_tag="IWJ07_001935"
     CDS             11916..12254
                     /locus_tag="IWJ07_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAP9333510.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12333..13040
                     /locus_tag="IWJ07_001936"
     CDS             12333..13040
                     /locus_tag="IWJ07_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAP9333511.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(13143..14624)
                     /locus_tag="IWJ07_001937"
     CDS             complement(13143..14624)
                     /locus_tag="IWJ07_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9333512.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4659 a         2531 c         3150 g         4333 t
ORIGIN      
        1 aaatgtcatt tttactgtat aattcatttg attatccatt ttttattcat caaaattact
       61 gaaaaaagaa aggaagaaat aaatttgaag aaaacaaatt cttcaggaaa accgaaacgc
      121 gacttcacta aattatcgac accccatacg tatgtcatta tttttggtgt agtcatcttt
      181 gcatggatac tgacttttgt cgttccagca ggaaagttca gcacgcaaga tattgaatac
      241 aaagatgcaa atggcgaaac aagtactcgt actgttttac gtcaagattc cttccgttat
      301 gcttacgagc tggataagtc gtatgtgttt gatcaattag aagaactgca agatcatcca
      361 gcagaacgtg aaaaactgga tgttccggaa aaaggattgg aaaaagtcat tgctgacgga
      421 gaaaaaaatc ttacgcagga aaaactagat gaaatctcct tgacagatga tgtgctttac
      481 gatcagtacg gcgaaaatat ctatgatact tctaaaaaat tgcataaaac agccaaaata
      541 tggggaacag atgattttgg cggctttggt ttcttaaatt ttgtttttga aggactagta
      601 tctggagata agtatggatc tgctgtaggg atcgctgcat tgatccttgt tgttggaggt
      661 gcttttggta ttattatgcg taccggagcg atcgacgcag gtatctatgc gtttatcagt
      721 aaaactaagg gactggagcg tttagcttta ccgctattat tctttgcttt ttcattcggt
      781 ggcgctacat tcgggatggc agaagaggta attccattct caatggtgat ggttccattt
      841 gtcattgcgt taggatatga ctcgatcgta gcagtaaccg tgacttatgt cgcatctcaa
      901 gtcggaaatg cgacctcttg gatgagcccg tttagcgtag cggtggctca agggattgct
      961 ggaatccccg tgttgtctgg tgcgacattc cgattgatta tgtgggtagt tgttactgca
     1021 ttagccgctg gttatatgat gatatatgca gaaaaaatcc gtaaaaaacc agaaagttcg
     1081 ttgacttaca aatcagatga ttatttccgt tctcatatca aaaagacatc tgatgaaaat
     1141 aaaaaattca tgcttgggca taaattgatc ttattagaga tgttagttgt ccttgtctgg
     1201 atcgtttggg gcgtgactca aaaaggctat tacattccag aaatcgcttc tcaattcttt
     1261 gtgatgggat tagttgctgg aatcattgct gttcttttca aattagatgg aatgaaagtc
     1321 aatgacatcg cttcttcttt ccaatctggt gcagctgatt tagctggtac agcaatcgta
     1381 gtaggtatgg ccaaagggat tcttcttgtc ttgggaggat ctgatgcatc tgttccttca
     1441 gctttgaata cgattttgca tgggatcggg acggcgttga ccggtgttcc agctgtcatt
     1501 ggtgcttggg cgatgtacat cttccaaagt ttgttcaact tggttgttac atcaaattca
     1561 ggacaagcag ctttgactat gccaatcatg gctccactgg cagatttagt aggtgtatcc
     1621 agacaagtag cagttcttgc ttatcagctt ggttcaggtt ttatggacgc ttttacaccc
     1681 gtatcagcca gcttgattgg tgtgctaggt gttgcacgga tcgaatgggc aaaatgggca
     1741 agattccaaa tcaagatgca aggattcctg tttgttttag gaactatttt catcatgatt
     1801 gccattgcaa taggtctgca gtagtactgg agacctgtaa ataaaaaagt aatccaaaca
     1861 gtagataagg actccaagaa ccgtcatttt acggtaaaat caaaggagta aagatcctat
     1921 cgtttggatt attttttaag atcctatcat cagttatact taaagaaaga ggaaaaatgt
     1981 atgaaaatca ttcgacaaat tgaagtgttt gcaccagatt acttaggaaa aatggatgta
     2041 ctgattgccg gtgggaagat tttggctgta gaagaacagc tagaaggcgg ctacgaagga
     2101 gtggaagtag aagaactttc aggtgaggga aaagtactaa ccccgggttt cattgactgt
     2161 catttccata ttttaggtgg aggaggagaa ggcggttatc aaaatcgaac gccagaagtc
     2221 acattgagcc aattgactac agctggtgta acgacagttg tcggttgttt gggaacagac
     2281 ggagaaggta gagatatgac tgcgttgatc agcaaagcga aaggtctgga agcagaagga
     2341 atctccactt atgtatacga aggttcgtac cgattgccgg taaaaacagt aacagattct
     2401 atcatcaaag actttttgac aattgataaa attatcggaa tcggagaaat cgcagtatcc
     2461 gatcaccgtt cttctcagcc aagttttgaa gagttcgctc gtgcagtagc agatgctcgt
     2521 gtaggaggca tgttatccgg aaaagctggg atcatcaatg tccacttagg cggaggaaaa
     2581 agaaagctag agctattgac acgaatcgta gaagaaacag aaattccgat cacacaattt
     2641 ttgcctactc atgcaaatcg cacaccagaa ttatttgaag cttgcgtggc atttgccaaa
     2701 cgtggtggca cgattgactt cactgctagc gaagatcctg atttttggga gaagacagat
     2761 ggtgaagttc gcttcagcaa agcactgaaa cgattgatcg aggaagatgt ttctttagat
     2821 aatttcacga tgacctcaga tggacaagga agcttgcctt attttgatga aaacaatcat
     2881 ttcctagggt taggagtagg aagtgccaag gcattgctag tagggatcaa agaagctgtg
     2941 caaaaagaaa gtatcccttt ggaaatcgcc ttacgagcta taaccagtaa tccagcacgt
     3001 atattgaaat tggataaaaa aggaaaaatc gaaattggtg ctgatgcaga tctgtgtatc
     3061 ttggataaag aaacgcttga tattgataca gttatcgcta aaggagaaat aatggtccaa
     3121 gaaaaagaag tcaaagtatg ggggactttc gaaaaaagtt tttaatcaac agctctaact
     3181 atctttttta gaaaacgagc gttctgtttg tctgtatctg ttgttgatgt taccctgaag
     3241 gtagatcaat aaacaaacta agaggaggaa gcacaatggc agatttaaaa ggacgttcta
     3301 aagatatgaa agataaagta gtcggtgaag gaaaagaagc atacggcaaa gcaacagacg
     3361 acaagagcaa agaaacagaa gggaaagcac aatcactagg tgctgacatc aaagaaaaag
     3421 ctcgaaattt aggcgacgaa gtcaaagaca agttttctcg aaaagacgaa aaaaaataaa
     3481 aaccacttat tactaattga atcaagcaaa taaaaaatcc gaaccatatc tgcgagacag
     3541 atatggttcg gattttttat ttgatcatta tcctgttttt taaacttcga ttttttgaaa
     3601 ttttttattt tttagttcaa agacttcatc acagcttttt gctacttcaa tctcatgagt
     3661 cacgatgata atacagcgat tttgttcatg ggcgatttct tggaaaagtt tgacgatatc
     3721 ttgtgaagtt gtttcatcta gatttccagt aggctcatcc gctacgatta gttgatgttc
     3781 acagcagata gcacgaacga tagcgactcg ctgctgctga cctcccgaaa gtttggtcac
     3841 ctgttttttt gctaatattt catcaattcc tacacgagcc agtcgatcta aagcaacttg
     3901 tcgataattc tcttgcttgg tcttagtgat ttccattgct gctatgacgt tgtctagagc
     3961 cgataaataa ggaagcaagt tgtaagcttg gaaaataatc gagacatcat ttttacgata
     4021 acttcttaac ccgattttgt tcagtgaacg atcttgatag tatacctttc cttcttttgg
     4081 tgcatctagt ccagaaataa gcgaaagaaa agtcgttttt ccagaaccac ttgctcctaa
     4141 aatagcgtac atttttccag cttcgaaatt cagttctaca tctttataaa gcgcatcttg
     4201 cggatcttga taccaaaatc ccaattgttc tgtccgtaac atcaaattcc tcctttagtt
     4261 taataggatt tttcttgggt ttaatcttaa tatccctata gaagctaaaa agacagaaaa
     4321 cagactgatt ccaaatgcta ctccggctaa tagtcccaat tgtgcaggtt tgacattgat
     4381 ttccaattcg ctgacttgat cagaaacagc aaatggatta ttcgtggttc cttgttgacc
     4441 atcgcgttga ccgccttgtt cttgtctgtt acctggcatt tgtccaccgc caggagcttg
     4501 ctggttttgt gtcgtggttt ctgtctgttg gtcaagtagc tgctgtccca cggcattcgc
     4561 gacgacgttt ccgctaagcg aagcaactcc taatgcaaga atcatacaaa tagccacttc
     4621 ggtaaacaat tggagaatga ttttacttct ggattctcct aaagatagca agacgcctaa
     4681 ctcatgccga cgctcccgaa tggtaatcat aatgatcaga gtaagtatga taataccggc
     4741 tacagccact agtaaaacaa catttttaga aaaacttgcc acgttgttta aaggttccaa
     4801 cattgactga tacatggaat catttgattg taagctaaat gtatctgtgt cgatcagctt
     4861 ctcagcttct ttgagaaact catccatttt atctggatca gatagagtgt aaatagcaga
     4921 atctatcgtg ttatcgtcag atgtcccatt tagttcattt gcaaaagtgt aagaagtgta
     4981 aagtgtattc gacggattca tgaagttgaa tttcataccc atactgccgc ttgtttcgct
     5041 ggattcataa attcctttga tcgtcatttc ataagtggta tcttcatctt cagtactcgt
     5101 gattgtgaaa gtatctccga ctgccaggtc atttgtttct gctagagtag aatcaatgac
     5161 tacattgttt gtgcctttgt cgctgtcatc tattccttca ccatccgtga tttttgctgt
     5221 tccatcagaa aaattactat tttgtgcagt tgctgacaca ccactgattt gaaagtctgc
     5281 ttgagtcatt tgcggattcc cgcccattcc tccagcaggt tcaccgcctg catcagtagt
     5341 ttctgaactg tcttcacttg aatcagaact agtgatggca gttattccat cttttgccat
     5401 agctgaggcg gaactttcaa aggaataact ctttacatta tctaattcag ctattttttc
     5461 cgcgtccgtt aaggaaactg gtgtaagaga aaaactgcct ggatctggac gttcttcagt
     5521 ggtagaaccg cttgaagaag tttgttcctg ttttttaaat gaagcttctc gattggcact
     5581 taaagtcact gttgctccaa cttctttttg tgcttgattc gctgcttctt ctgcagcgct
     5641 tcgaatcgtg aggccagcta gcacaaaaat aagaatggct gtgaacacgg caaacaataa
     5701 caatgaacgt ccccatttta cttttgtact tttccatgca cgtttcataa aattcatatt
     5761 ttgcatccct cctagtagat gcatttgata atagacaacc tatcttaaag ctagcttaaa
     5821 tcttatgctg tttgcacacc atcaacatga ggttctgtat atataaaaag tcactatagt
     5881 tattgcaaaa aatgtggtaa aatgaaaaaa gtatgtttca aaccgacgaa gaagaaatga
     5941 aagaggaata gcaaaagaaa tcctagatgc tacagaggga tttttacgtg catagttgtt
     6001 ctaacaattg ctgatactag cgatttagtt tatttgttgt ataataatgg ggaacagctg
     6061 aatacttata taagtattct ggctatagcg tttcgcaaaa gatccatttt gttaatgaag
     6121 tatatccaaa ataaaatgcc catccaactg tcagaacgcg gatgtgctgg taattttcct
     6181 gctgattctc cataagttga gaaatagtga ttttgcatat gcttcattgg aatggctcat
     6241 atatttagat aaagaagaaa atcgacatct ttaaacttgg atgtcataag aagaggtgaa
     6301 gtttgtgagt accattaaaa ttatcccatt aggcggcgtt cgtgaaaatg gaaaaaacat
     6361 gtatgtcgca gaagtgggcg aggatatttt tattttagat tgtgggctaa aatatcctga
     6421 aaatgagttg ttggggatcg acgtcgtaat ccctgatttt acgtatttag aagaaaacat
     6481 tgatcgtgta gctggtgtct ttttaacaca tggacatgca gatgcgatcg gggcacttcc
     6541 ttacttgttg tcaaaaattt ctgtaccagt attcgggaca gagttgacga tcgagttagc
     6601 aaaattgaat gtcagtcgta atgaagcagc caaaggattt aaggatttcc atgtggtaga
     6661 tgagcataca gaaatagact ttggtgaaac agtagtgagc tttttccgta ccactcatac
     6721 gataccagat tcagtgggga tcaatctgaa aacaaaagaa ggtagtatcg tttatacggg
     6781 agattttaaa tttgaccaaa cggctatccc aatgtatcaa acagattatg cacgattggc
     6841 ggaaatcggc aaagaaggag tcttagcctt actaagtgaa tcggcaaatg cagaaaatcc
     6901 tcagcctgta gcttctgaag cccaaatagc agatgaagta tttgatacga tcaaatattg
     6961 ggaagggcgt atcatcgtcg cttgtgtagc aagtaatttg caacgagtgc aacaagtatt
     7021 ggatgcagca gaaaaagcac aacgaaaagt tgtcttgact ggtcaagatt tcgaacgaat
     7081 tattcgcaca gcgatgcgtt tagaaaaact tcaattacca agtgaagatc tgcttgttac
     7141 gttgaaagac atgaaaaact atgatccaga agaactgatc attttagaaa ctggccgaat
     7201 gggtgaaccc atcaagtctc tgcaaaaaat ggcaaacggt acacaccgta atgtcaagat
     7261 tgaagaagga gatttggttt atatcactac tacgccaagc atcgcgatgg aaaccatcgt
     7321 ggcgaaaacg gaagatatca tttatcgagc tggtggaaca gtgaagttga tctctgagaa
     7381 tatgcgcgtt tctggccatg ccaatcctaa tgacttgcaa ttaatgatca atttgatgaa
     7441 accaacatat tttgtgccag ttcaaggaga gtatcgtgaa ttagctgctc atgcagattt
     7501 ggctcatgca gtagggatgc catacaaaaa tatctatatt actggacgcg gagacgtgct
     7561 ggaatataag aacaagcgta tgagtgttgc aggaactaca agtgcagaaa atgtaatgat
     7621 tgatggatta ggcataggtg atatcggaaa cattgtttta cgtgatcgaa aaatcttgtc
     7681 agaagacggg attttcgtag cggttgtcac tatcagtcgg cgagaaaaga aaatcatttc
     7741 aaaacctcaa attacttcac ggggattcgt ttacgtaaaa gctagccgtg atctgatgaa
     7801 agaaagcagc aatatgattg aaacaatcgt tgagaaacat ttagaaagca atgattttga
     7861 gtggagcaaa ttaaagcaag acatccgaga tcaattaggc cgttacttat ttgaacaaac
     7921 aaaacgccgc ccagtgatct tgccagtcat tatggaagct actcaacgtc gaaataaaaa
     7981 ataaacaaaa agagtaagat gaacctctct atagagtgct cgtcttgctc tttttttgcg
     8041 aaaaacaaat aatttttgaa tacagcatcc ctcgctttat aataaacgat agaataaagg
     8101 gagtgaatca atgatgagtc aaacaaaagt cgaacgttat ttagaagaac aaaagatcag
     8161 ctatcaacca cttgatttta gtcatgtggt agaagaatct ttcacagaag aactacaaaa
     8221 aagagggatt gatccagctt tgatatgtaa aactctcgta gtgaaaggaa acaaaactgg
     8281 ggtgatcatc gcattgattc ctttgaacga tcacttagat tacaaaaaaa cgagacaagt
     8341 gacgaaagat cgtaaagttg gttttcctgg aatggatttt gtattaatgc atacaggata
     8401 tccccacgga gcaaacaccc ctattggaat aaaactcgca catcctgatt attgtttttt
     8461 agcagacagt tctttaaaag aaaaagaaga agtaattgtg tcaagcggtg aaatcgggag
     8521 aagccttcgg ataaaagtag ccgatttaga aaagatcgtc catcctattt acgctgattt
     8581 aattagttaa gggacgagaa gagaagctaa gcagagcaga gacctcaaac ttgagattat
     8641 atctgcttgg tacttggtgc atcctgtatt atctgttaca attgctttgt acatactgta
     8701 ctatcaatcc aatgaaaata tatttagcag ctatattttt tcattgatgt gcaagtgatg
     8761 aaatcgtttt taataaaaaa gatacagaga aaacgcgagg tgtaaaatat gaaaattacg
     8821 tatcatggac attcctgttt aacaattgaa atggaaaatg gccaacgcgt gatgattgac
     8881 ccgtttatta caggaaatcc attgatagac ttaactgcag aagaagtaaa gacagattgg
     8941 ttgctgatta ctcatggtca tagtgaccac ataggagata tgattcctat tgcaaaagaa
     9001 aatcaagcca caattatcag tattgttgag attgctgatt atgcgaacag cagaggagta
     9061 gattcattcg gtatgaatat cggtggaaaa catgcgtttc cattcggcgt ggtaaaattc
     9121 gtacatgcac agcatagttc aagctatgaa gtagacggta tcgttcagta tatgggcgag
     9181 cctagcggaa ttattattca agcagaagga aaaacgattt atcatgcagg ggatactgct
     9241 tatttcagtg atctaggatt gcttgcagaa gaattcgata ttgatgtcgc tttcttgccg
     9301 attggcgata actatacgat ggggccagaa gatgcagcaa aggctgcgca aacaattcgt
     9361 gccaagaaag tagttccaat ccattacaat actttccctg tcattgaaca agatcccgaa
     9421 tcttttgtag agttactgga agatagtgaa ggaaaaataa tgagcgtctc ggaaacgctg
     9481 gaagtgtagg cagaaaatgg cgacaaaaca tgatttgatt ttagaatata ttgaaagttt
     9541 acctgttggc aatcgaattt ctgtaagaag catcgcaaaa gaattgaatg tcagtgaagg
     9601 aacagcgtac cgtgcaatca aagatgcaga aaatgtcgga ctagtttcaa caattcaacg
     9661 agtagggacc atccgtatcg aacgcaaatt aaaaaaacat attgaacgtt tgacatttgg
     9721 cgaggtcgtc aggatcattg aaggggacgt tttaggcggg gcggcaggat tagacaaggt
     9781 cttgaataaa ttcgtcatcg gcgcgatgac taaacatgcc atggaacgct atattacgcc
     9841 aggatcacta atgatcgtcg gcaaccgggc ggaagtccaa aaattagcat tggaagatgg
     9901 cgccgcagta ttgatcacag gaggattcaa tacgactgct gagattgcaa agctagctga
     9961 tgatcttgaa atgccgatct tacgcacctc ttatgataca tttacagtcg caaccatgat
    10021 caaccgagcg ttaagcgacc aattgatcaa aaaagatatc atgcttgtca gtgatatcta
    10081 tatgccagct gaaaagactc attatttgca gcagacagat acgatttctg attatcagcg
    10141 tttgtcagaa gaaacgcagc attcgcgttt tccagtcgtc aaccgccatc atcgtttgat
    10201 ggggattgtt acggcaaaag atgttttagg gaaatcgcca aaccagatca tcgatcgggt
    10261 gatgacgaaa gaaccaatca gtgtaaagaa aacaatgagt attgcctctg ttagccatca
    10321 aatgatctgg gatgggctag aagtcatgcc ggtcgtttcc gatgacttga cattggaagg
    10381 cttgattacc cggcaagatg taatgaaagc gatgcagctt gtccagcgac aaccgcagat
    10441 tgcagatacg atttcggatc agatatctgg aaatatccat acgattgata ctgaccgtga
    10501 aggaaatcgt ttggaaaacc ctaagttcaa attcgtagta gcaccacaaa tggtcaatgg
    10561 cgttggaacc atttcttttg gcgtacttag cgaaatcatt tctgatgtcg cacaaaaaac
    10621 aatggtgatg aaccaaaaac gaaatatctt gattgaacaa gtgaacctac attatttgcg
    10681 gctgatccaa ttggaaagcg aattagacat tcgtccacgt gtattggaaa tcggcagacg
    10741 ttccgctaaa ctggatatcg aagtgtttat tgaaaacacc atcgtttcca aagcaatcgt
    10801 tgtttgtcaa gtaatggaac gttcttagaa agcaggagtg aaatgacagt acagcaagaa
    10861 atagtagata caataaaaaa atacgataaa atcattatcc acaggcattt gcgccctgat
    10921 cctgatgcat taggatcaca agtaggatta gccgaactat tacgggaaag tttcccagaa
    10981 aaagaaatac gtcaagtagg tgaaacgatt gaagggcttc gctttttagc agaaatgcaa
    11041 gaagttgaag atgtcttcta ccaaggagcg ctagtcatcg tgactgatac agctaattca
    11101 cccagaatca gtgatcaaag atatcagttg gcagaaaaac tgatcaaaat cgaccatcat
    11161 cccaatgatg acccttatgg agatcttgtg tgggtaaata caaaagcaag tagctgtagt
    11221 gagatgattg cagaatttgc tttgatgttt cctgaatctt taaagatgaa cgttaaggct
    11281 gcacggttac tttatgcagg aatcgtcgga gatacagggc gctttttgta tccctccacg
    11341 acgtcccgta cattagagat tgcttcgata ttgagaaatt atccatttga tgcaagtgct
    11401 ttgaacagag agattgaaca aatgccgatg aaagtcgcta aactttccgg ttatctttat
    11461 caaaatatcc aggtggatga aaatggtgca ggaagaataa tccttccgca agaaattctt
    11521 gataaatatg gaatcgatga ttcagagaca gcttcgatcg tttcacttcc tggagtgatt
    11581 gacgaagtgc tggcttgggg gatttttgtt cagcagcctt cagggtatta ccgcgtacgt
    11641 ttacgttcta aggggccgat catcaatgaa ttggctaaac gccatcatgg aggaggccat
    11701 ccattagcaa gtggtgcgaa tgcaaaagat ttagcagaag tagaggaaat ctataaagag
    11761 atccaagata tatgccaatc gtataagagc agaaaaacag aatgatactt cgttcgaaaa
    11821 cgtgcctttt gtagtaaaaa tcacaaatgt atcttttcaa gatcttcttt ttccgttaca
    11881 atagtgctca ctaaagattt taaaggagaa agaagatgac attaccaaat tgcccagaat
    11941 gtggctcaga gtacacatat gaagatcgag gcttgttgat ttgcccagaa tgcgggaacg
    12001 aatggaacaa agaagaacaa gaaactgtca tagaagacgg attagtggta ttagatgcaa
    12061 atggaaaccg tctgttagag ggcgatagcg tgacagtgat taaagacttg aaagtaaaag
    12121 gtgcgtcagg tgccattaaa caaggaacaa aagtcaagaa catccgttta gtagaaggcg
    12181 atcataatat cgattgcaag gtagaaggat ttggaccgat gaaactgaaa tcggaatttg
    12241 tgaaaaagaa ttaaagaata ttcaaggtct tttcacattc ttttcctact actaatgata
    12301 aaataaagta aacgaaaagg agtgaaacga aaatgtatgg ttatggaatg tatggttttt
    12361 tagatccgac atttcttttg gtcattcttg gattagttat ttccggaatt gcgtcagcgt
    12421 acgtcaatag tactttccga aaatacgatc aagtgagaag caaaaacaat gtgactggta
    12481 cacaagcagc acaatatata ttacaaagtc aacagataaa taatgtcggt gttcaacaaa
    12541 ttgccggaga tctcacagat aactacaatt caggtaataa gatgttgagt ttgtctcaag
    12601 caactgcaca gtctacttca gttgctgcga taggtgtagc agctcatgaa tgcggacatg
    12661 cggtacaaga tgcttcgggt tatgtcccat tgaagattcg tgcagcactt gttccagcag
    12721 ctaactttgg ttcgatgatt tcgttcccct tgattatggt aggtgtattg cttagctgga
    12781 actctacgtt aatcaatatc gggattttcg ctttttcttt agcattactt ttccaattgg
    12841 tgacattacc agtcgaattt aacgcgtctc ggcgagcaat ccaaattttg agtgaaggcg
    12901 gactactgac ggaagaagaa gtaccgatgg caagacacgt attatttgca gcagcactga
    12961 cttatgttgc agcagcgctt tcgacattcc tgcagctgtt gcgcttgatc ttattgttcg
    13021 gcggcaaccg aagagattaa aataaagaag ttgtgaaatc agcgctttga tctgagctaa
    13081 taccgagagt tagctgattg aataccattt atttggaata agaggatgtg acaagaattt
    13141 tgtcacatcc tctttgtttt gttctcataa ccttttttca tatacagttc atgaaccgct
    13201 tttattaact tgccaaataa cactgtattg tgcgttgtat ccagatagaa agtctccgtt
    13261 tcatctgaaa ttttctcatg ctgatctgta atgatcaaat cagcatgttc tgaatatgcg
    13321 gtaattttaa cagcatcttt gcgaaataac tgctgcaaat ggctttgaag atgataattt
    13381 cctgtaaact gtttggttat ctgcaaatgg atgagtaact gctctttcgt ttctgtttgt
    13441 agtaagtgtt ggatcaattg actaaggtat tgactgattt ggtttctttc cccatttttt
    13501 gaaaaaattg tatcgatttt ttctcttaat ttcttttcct gtataaaaga actagaatga
    13561 ttggacgtgg cttctggaaa gcaaagtgta gaaaaactac tgctggattc acccaacagt
    13621 tcgaaaaaaa caactgccaa cacaagaaaa taaaaataaa ttggtatttt attttctaaa
    13681 tggttgagag gaaactgttg agttaagtaa gtcatcaatt gaccgacttg tcgacaaata
    13741 aggtttttgc ttgtggaaaa taaaaaacca atctcctgtt tttgtcgaaa agagatttga
    13801 tttggaaaaa aacaatagaa aaaataatta aagtaggctc tctctgtttc tgtctcttca
    13861 caagggcgac tcactgaaaa agaagtaaag tattctgaat aatcatgtgt atgctgcaat
    13921 aagtccatca attcagtgat ttcagaagaa gtttttggaa aatatgcctg ttgtttgttt
    13981 cgtaatatga ataaagttaa cagcaagtag atattttttt cttgttttga aattttttct
    14041 atttgctcat ctgtactgtt catttttagt gcatttaatt caagtgagga aaatggccaa
    14101 tcgttacctc caaatgtatc tgaaagaaaa aagtaaagat agagacggat agataactca
    14161 tttccttcaa gagtacagca attttttaca gataggatcc gtaaatggaa tttttttaag
    14221 gaatgattga gctgttttgt cagtttttcg agataggagg gcgatatata aagttgttca
    14281 agtaaatatt tttttggtac agcagtttgt ggataattga ttagtaacat caaatagtta
    14341 aaacgattgg attcttttag gtagcataat tttaacttag aaaaaacagc taacttttta
    14401 gtttgatcgg tttctttggt ttggatctct cctttatgat tagaataaat aaattcatca
    14461 atcaataaaa attgcttgag tgatacattt atttttcgga tcagttcttt gattgtccgt
    14521 tttggaatat gtaagtcatt ggataattct tgtaaagaac aattattttt ttgaatgacc
    14581 atttgtagaa caagtaactc tttttttctt gcttcaatta gcataaatac ctccttttta
    14641 ttctgttttt gtatatagtt agacaaaaac tgt
//
LOCUS       DACDPS010000059        14377 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.059, whole genome shotgun sequence.
ACCESSION   DACDPS010000059 DACDPS010000000
VERSION     DACDPS010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.059"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            457..1587
                     /locus_tag="IWJ07_001938"
     CDS             457..1587
                     /locus_tag="IWJ07_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP9333513.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            1679..2434
                     /locus_tag="IWJ07_001939"
     CDS             1679..2434
                     /locus_tag="IWJ07_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP9333514.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            2492..4531
                     /locus_tag="IWJ07_001940"
     CDS             2492..4531
                     /locus_tag="IWJ07_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAP9333515.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQATRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(4640..5791)
                     /locus_tag="IWJ07_001941"
     CDS             complement(4640..5791)
                     /locus_tag="IWJ07_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP9333516.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(5945..6475)
                     /locus_tag="IWJ07_001942"
     CDS             complement(5945..6475)
                     /locus_tag="IWJ07_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333517.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(6574..6924)
                     /locus_tag="IWJ07_001943"
     CDS             complement(6574..6924)
                     /locus_tag="IWJ07_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAP9333518.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(7081..8610)
                     /gene="ahpF"
                     /locus_tag="IWJ07_001944"
     CDS             complement(7081..8610)
                     /gene="ahpF"
                     /locus_tag="IWJ07_001944"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAP9333519.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(8620..9183)
                     /gene="ahpC"
                     /locus_tag="IWJ07_001945"
     CDS             complement(8620..9183)
                     /gene="ahpC"
                     /locus_tag="IWJ07_001945"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAP9333520.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(9327..9989)
                     /locus_tag="IWJ07_001946"
     CDS             complement(9327..9989)
                     /locus_tag="IWJ07_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9333521.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            10173..11819
                     /locus_tag="IWJ07_001947"
     CDS             10173..11819
                     /locus_tag="IWJ07_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP9333522.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            11810..12892
                     /gene="uxuA"
                     /locus_tag="IWJ07_001948"
     CDS             11810..12892
                     /gene="uxuA"
                     /locus_tag="IWJ07_001948"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP9333523.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(13000..14377)
                     /locus_tag="IWJ07_001949"
                     /pseudo
     CDS             complement(13000..14377)
                     /locus_tag="IWJ07_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
BASE COUNT         4726 a         2619 c         2709 g         4323 t
ORIGIN      
        1 atttgcaacg ggattctttc aacttatttg ttattttttt ctaaaaaata ttaaagattg
       61 ttaaatttca ctatttcatt tcaaattcca ttgcctaatc attaggaaaa gtctatcatg
      121 aactcgtaaa tagaatatag taataacctt gttaggtgag gctcctatac aaacataggc
      181 tgttgcccag aaacgtcgaa agtcgccaat gggtaaaaca gaaactgttg gattaaggct
      241 ttttccaata gctaagaatg ttttctttac gttgtatagt gctaaaactc ggacgatgag
      301 atgatgctag gtcagaattc ttgttcgaaa aggtttctga ccttttcttt atatttttgt
      361 gactgactta tttgatgaga caaactcgct cctcacctga caagtggatt gctgtgtgag
      421 gaggagatgc ggtgaaaaaa gatcgattgg tggattttgg gtccttattt gactttgtcc
      481 atgatcggtc ttttagaagt atacagtgcc agttcttatc gtttattgca agccgatgaa
      541 aatacaaaaa gtctattatt gcgtcaacta attttcatat ttttgagttg gagcgtgatc
      601 ttcttagctc gttcagtcaa actacactat ttacttcacc ctaaaatagc aggatacggt
      661 ttagccttat cgattttctt tttagtatta gtaagaatag ggatattcgg tgtcactgtc
      721 aacggcgcac aacgttggat ctctctgttt ggcattcaat tccagccttc tgaactggca
      781 aatctttttt tgatttttta tttaagctgg ttttttcgtg acggaaatag tagcccaaaa
      841 gatctaaaaa aaccattcct gattacagta ggtataactt ttctgatttt atttcagcca
      901 aagattgctg gagcattgat gatcctttcg attgcgtggg tcatattttg ggcagcggcg
      961 gttccattta aaaaagggat ctatctaatc gttacttttt ctgcattgct gattggagca
     1021 gcaggcgggg tattatattt aggaaataaa ggttggcttc cacagatgtt taatcatgct
     1081 tatgaaagga tcgcgacttt aagagattct tttatcgata gtcatggtgc gggctatcaa
     1141 atgacccatt cgttttatgc actatataat ggtggcatat ttgggcgggg attaggaaat
     1201 agtatcacaa aaaaagggta tcttccagag acagaaacag attttatttt ttcgattatt
     1261 acagaagagc ttgggctaat cggtgcattg tgtgtgcttt tcttattgtt ctcattatgc
     1321 atgagaatct tttgtttaag cagtcgctgt aaaaatcagc aagctggatt gtttctttta
     1381 ggattcggaa ctttgctatt cgttcagacg ataatgaacg tgggaagtat cgcggggctg
     1441 atgccaatga ctggtgtgcc attgccattt gttagctacg gaggaacaag ttatctgatc
     1501 ctttctttag gaatcggaat cacgttgaat atttcttcaa agatacaggc cgaagagttg
     1561 gcgctctatc gacctgaaaa acaataaaga gaaactgaga aactctcagt ttgaattatc
     1621 ataaagtaca atcttttatt tttcaagcga gaggttcttt tagaaagagg aattcgcaat
     1681 gaaagtatct caaaaaatca tattatctat tttgttactg cttacattag tagttggcta
     1741 tttttcgatt gagtttatcc atggattttc gtctgcaaaa cagagttcaa ctgtgaaaaa
     1801 ggtagagccg aaaagtatcc ctaccacact aaatgtggct ttgattggtt cagatgctcg
     1861 ttcgaaagac gaaaacggtc gctcggattc acttctggtt gcacaataca accagaaaac
     1921 acaacaagca aaactgatct ctatcatgag agattcatat gtcgatattc caggttacgg
     1981 aatgaataag ataaatgcag cttactctta tgggggagtt gatttattaa atcaaacatt
     2041 aaaggaaaat ttcaaatttg aagctcccta ttatgcaagt atcacatttc aagattttat
     2101 cgattgcgtt aatgaactgt ttcctggtgg agtcaagatc gatgcagaaa aatctctaga
     2161 tttagatggc gtatatataa acaaaggaga gcaagtgatg gatggtaata ctttactcca
     2221 gtatgctcga tttcgggaag acaaagaagg agacttcggg aggatcagaa ggcagcagca
     2281 agtgatcaaa gctatctctc agcagctgaa agacgtaact tcaatattca agctacccaa
     2341 agcggttggg aaattacttg gtagtatcca aacgaacctt ccagaaagtg tgcttctgga
     2401 ttgtggtatg gatttcctca aagacatatg ttagaataaa caggtataaa tagtgaaata
     2461 aaggaatgac aagcaagaga aggaggaaaa aatgaaaaga agtgacaagc acggcaaaaa
     2521 tcgaacaggc gcttatattg ccggcgcagt gatcttaata gcagctgcgg gtggcggtta
     2581 tttttactac cagcactatc aagaaaccca agcagtagaa gctggagaaa agacagttga
     2641 gcaatttgtc caagctttga acaagggaga ttataacaaa gctgcagaaa tgacatcaaa
     2701 aaaggcggca aataaaagtg cattatctga aaaagagatc ttagataaat accagaatat
     2761 atacggtgct gctgatgtca aaggacttca gatatcgaat ctaaaagtag ataaaaaaga
     2821 cgattctact tacagttttt catataaagc aaagatgaat acctcattag gtgaattgaa
     2881 agatctttct tataaaggaa cattagacag aaatgatggt caaaccacga tcaactggca
     2941 acccaacttg gtttttccag aaatggaagg aaatgacaaa gtaagcctga ccacacaaga
     3001 agcagcaaga gggaatatta tagatcgaaa tggtgaacca ttagcaacaa caggcaaact
     3061 aaaacaatta ggagtcgttc caagtaaact tggagatgga ggcgaaaaaa cagccaatat
     3121 caaagcgatt gcttcctcct tcgacttaac agaagatgct atcaatcagg cgatttcaca
     3181 aagctgggta caacccgatt actttgtccc attgaaaatc attgatggag caacgccaga
     3241 acttccagct ggagctacca ttcaagaagt agacggcaga tattatcctt tgggtgaagc
     3301 agctgctcag ctgattggtt acgtgggaga tatcacagca gaagatattg ataagaatcc
     3361 agaattaagc agtaatggaa aaatcggtcg ctcaggtttg gagatggctt ttgataagga
     3421 tcttcgtggg acaacaggtg ggaaattaag cattacagat gcagacggtg tagagaaaaa
     3481 agttctgatc gaacatgaag ttcaaaatgg caaagatatc aaattgacaa tcgatgcgaa
     3541 ggcacaaaaa acagcttttg acagtctagg aggaaaagct ggatcaactg ttgcgacaac
     3601 gccaaaaacc ggtgatcttc ttgcgcttgc tagctctcca agctatgatc caaacaaaat
     3661 gacaaacggg atctcacaag aagattacaa agcttatgaa gaaaatcctg aacatccatt
     3721 catcagccga tttgcgacag gttatgctcc tggatctacg tttaaaatga ttacagcagc
     3781 aatcggtctc gacaacggca ctatcgatcc aaatgaagtg ttgacgatca acgggcttaa
     3841 atggcaaaaa gacagttctt ggggatctta tcaagcaacg cgtgtcagta gtgatgtatc
     3901 acaagtagac ttaaaaactg ctttgatcta ttccgataat atatatgcgg cacaagaaac
     3961 gttgaaaatg ggtgagaaaa aatttcgtac aggcttagat aaattcattt ttggtgaaga
     4021 ccttgatttg ccaattagta tgaatccagc acaaatttct aatgaagata gctttaactc
     4081 agatatcttg ctagctgata ctggatatgg acagggcgaa cttctaatta ctcctatcca
     4141 gcaagcagca atgtattctg tttttaccaa caatggcaca cttgtctatc ctaaattgat
     4201 tgcagataaa gagacaaaag ataagaagaa tgtcatcagc gaaacagcag tacaaacgat
     4261 cgtgccagat ctgagagaag ttgtgcaaga tgtaaatggt acagcacatt ctctttctgc
     4321 tttagggatt ccgttggcag cgaaaactgg tacagcggaa atcaaagaaa aacaggatgt
     4381 aaaggggcaa gagaacagtt tcttgtttgc tttcaatctt gataaccaag gttatatgat
     4441 ggtcagcatg ttggaaaata aagaagatga tgattcagca accaaacgag catcggaact
     4501 attgcaatac ctcaaccaaa attatcaata aaaaattgct ttttaagtac agtaaaagga
     4561 ctgtggcgtt atcgtcacag tccttttttc aaataaacgg ttttcacgat gtctctttca
     4621 caacttcttt ttgatttact cacacttcat aaactttttt tgtaaaatat ttgagaacaa
     4681 gtggagaaat aagtgtactt aataaaatca cgataactaa aggtgaatag tactgtggtt
     4741 cgatcaattg actttgctgt ccaatttgta aaatgatcaa agccatttct ccacgcgaaa
     4801 tcataccagc accgaccatc aatgaactat gttgagaaaa accggcaatc tgagcaccaa
     4861 aataacctcc aagcaatttg gtcaaaatcg ctacaagcgt caatatagaa ataaagagta
     4921 gttgttcact aaagtcagaa aaatcaactt ctaatcctac actgacaaaa aatactggta
     4981 taaatactgc atatcctaaa gcctctacat tgtggtatac ttcttcttta acctttgtct
     5041 gactgacagc tatcccagca aaaaaagcac caatgacaga gctaagccca acaagatccg
     5101 ctacgtaaga cattcctaaa caaataacta gcgatacgat gataatagaa gaatttgcgt
     5161 acactttttc tgcaaatgaa agtaggaaag gcgcaatcca tttgactaat aagaaaataa
     5221 aaacaaaata aattagctgt tcaatcaaaa caagcggtaa agaagcattg ctagaagctt
     5281 cccccgttag aaaagatagg ctgaaactta gaactaaaac gactaaaatg tcatctacca
     5341 cagaagcacc caaaattgtt gatccttctt ttgtattcac tacgtttagt tcttttaata
     5401 cttctactga aatactgaca gaagttgccg caagaataat cccaaagaaa atcgcttcat
     5461 ttcccgaaac ttggaatgct ttgcctgtca accagccaaa aacaaccggg aagatgatcc
     5521 caagcaaagc aacgaacata ccaggtcgga aatatttttt caaaagagat aaatcacttt
     5581 ccagaccagc taaaaacatt aataagatca caccaatctc cgaaaagtca tggactagaa
     5641 tatcaggttt gacccaattc aatccagctt ggcctagcaa aacaccaaca agtaattgtc
     5701 caatcactgc tgggatacct aatctccgcg agatatgtga gccaatcgtt gttgaaatca
     5761 atatcaggca taaaatacca ataaattcca ttctcatcat tcctttgtat aaaatatagc
     5821 ccgaacttag cataacatac attattgaaa aaataggaaa atatactcac aaaaaaacca
     5881 acaatccaaa agattttttt ggattgttgg tttttatacg gtctatttta ataactaaaa
     5941 atgattaacg tatagtcatt tttcttattc tgatgaaatt gataatctcc tggatgccca
     6001 tgaaaatcaa aataaagccg aatatttgga atagaagcat caaacttttg aatggattga
     6061 acaataagac gaatccgcca atcaacaaca gaatactact ataaacactc caacttttag
     6121 cttttgtatt catgctgaag aacaactgga acaagctgtt gatgataaca gctaatccaa
     6181 gaagaatcgg caggatagaa acgattgcag gtgcaaaagc atagacgatc agagctaaga
     6241 tcaggaaaaa aagaccactg aacagaccga tactccaaga acctgttttc cttttaaaac
     6301 ggtaagcctc aaaaagattc aacaacccaa gcaaaacaag ataggcggtg atcagataac
     6361 cgataaaatg aaaaacagca gaagggttta gcacaatcgc aatcccagaa ataatataaa
     6421 cagcagctct taataaggcg tatttttgaa tattttgttt aatcgtctca aacatataag
     6481 aaaacctcct ttgacttatt ttaacgtgaa ctagtcattt taccaaatta aatgttacaa
     6541 aatgagcgaa ggagaagaat atctctggaa aaatcaactt ctgaggatct tttccatttt
     6601 tttgcctttt gctagctcat cgatcatttt atctaaataa cgaatttctt tcatcaagtt
     6661 atcctcgatg tcttctactc gtacaccaca aacaacacct gtaatcaagg aacgtgaagg
     6721 attggggaca ggggcttccg caaaaaattg ttcgaaagtc acttgttttt gtagttgata
     6781 ttctagttct gtttgggaat aacccgtcag ccaacaaatg atttcatcta cttcctcttt
     6841 tatacgatct tttctctccg cttttgctac atacatagga taaacgcttg caaaacttgt
     6901 cgtaaaaata cgattttgcg ccataatcag tcacctttct ttttttcaaa ttatttacta
     6961 cttatagtat ataagaaaaa gagaagaaaa agcgattatt caaaaaaaca aaaaggattg
     7021 tgacaaaatt cttgtcacca tccttttttc cgctctctga ctttttacaa tcttcatagc
     7081 tcaagtgcgc atcagataat cgaatgctcc taaagctgca gtagctcctg aacccattga
     7141 aacaatgatt tgtttgtatg gactatctgt acaatcacca gctgcgaaaa cgccggggat
     7201 attcgttgcg ccatttttct cggtcactat ttcatttctt ggattcattt cgatcgttcc
     7261 atctagccaa tccgtattag ggagtaagcc gactagaatg aatactcctg aaacagctag
     7321 tgtatgcatc tccatcgtac ggcgatcgat ataggagaga gattctacct gattcgtacc
     7381 gctgatttct ttcgtcgctg catgtttgat aacagtgaca ttcgcaagag agactagttt
     7441 atcctgtaag atttgatctg cttttaattc tggtaagaat tctagaacat atacatgttt
     7501 tgctaaccca gccaaatcga ttgctgcttc aacaccagaa ttaccaccac cgataacagc
     7561 aatctctttt cctttaaata aagggccgtc acagtgtggg caataagcaa ttcctttatt
     7621 tttaaactct ttttcgcctg gtacattgat agaacgccag tgcgcacctg tcgcgatgat
     7681 ggcggttttt gctttaagtg atataccgtt agctaagcca atattaataa aatgttgctc
     7741 tttatttaag gaaacggcct gttgattcat cataatgtcg atagggtaac tacgcacatg
     7801 actttctact tgctgcatca attttggacc ttctgtgtaa gaggtaccaa tcatattttc
     7861 aatccctagt gtttcaacga cttgtccgcc gaaagaatca gcgacaagtc ctgtacggat
     7921 tcctttacgt gctgcataga tagctgcact actagctgct ggtccaccgc caatcactaa
     7981 tacatcataa ggttcttttt caaacaactc ttcttcaatc aaagggccag tgattttttc
     8041 aacaatttgt tcgatcgtca tgcgtccgct actaaattct tctccattta aaaacacagt
     8101 tggcactgcc atgatttttt tatcatcgat ttctttttgg aacatcccac cttcaatcat
     8161 cgtatgagta atatttggat ttaatacagc taatatattc aatgcctgta caacgtcggg
     8221 gcaattatga caagtcaagc taacataact gtcaaaatgc aatgtttgtg ttattttttt
     8281 gattttaaga ctcaaagatt cactgatttt cggtgcccgt ccactaattt gtaacatagc
     8341 cagtaaaaaa gaggaaaatt catgaccgag ggggatacca gcaaaaacga ttccactttc
     8401 tttgccaact ggattgatag aaaaactagg tgtgcgcatc gaatctcttt tttcccaaga
     8461 gattttagga gacatttctg ctatttcctt cgtaaattca gctagttttt gactattttc
     8521 actattatcc aaactaacag aaaaaacaat aggagattca atcagtgcta aatattgttc
     8581 gagattttgt ttagtttctt tgtctaacat atccaccacc taaatctttc caacaagatc
     8641 aaaacttggt ttcaatgttt cgccactttc tttccatttt gccggacaaa cttcacctgg
     8701 gtgggtacgt acatattgag cagcgcgaat cttatcaatc agtgcactag cgtcacgacc
     8761 gattccatct gcattgattt ccatagcttg aacgattccg tctggatcaa taataaatgt
     8821 tcctcgctga gctaatcctt gctcttcatc caaaacatca aaaatacgag aaatctgatg
     8881 agacggatca ccgatcatca cgtattcgat tttaccgata gcatctgatg tatcatgcca
     8941 tgctttgtga gtaaagtggg tatcggtaga gcaagaaaag acttcaacac ctaaagattt
     9001 caatgtactg tattgttctt gtaagtcttc taattcagta ggacaaacaa aagtaaaatc
     9061 agctggatag aagcaaagaa tactccagtt ccccataaga tctttttcac tgacttctaa
     9121 aaattctcct tgatgataag catgtgcatt aaattcttcg atttttttcc caatcaatga
     9181 catagtaaaa cctccaataa taattattta tttataatta ttgtaattta gttggagttg
     9241 caaagtcaat ggatattttg ttatttaata aagattactc aaaaacgaag gttaattata
     9301 aaaatagaag aggtcgcaag aaaatgctac tgctggaaat actcgggata acgttgagta
     9361 acagtttcct gttctttcag catcatgtgt aagtgcaatc ccgtttgaaa tcgagcttct
     9421 tctggttttt tatcaactaa agccttgagt aaatcataat gttgttccat gatcgtttcc
     9481 catggaagcc cctgaatggt cactcgtaac cagcggaaac gttctaaatg ggtattatgg
     9541 tcatctaacc agttccaaac ttcttttctt cctgcaattt cgaaacaagc ttcatgaaat
     9601 aaattatctg cacggaaaaa agcgcgttcg tcatttattt tgactgcttt ttcttgttct
     9661 tctaaaatcg tttctagcac tattcgtgtt tgtgtgtcca atttggaaca acattcaatc
     9721 atgatttgct cttccaactg ttctcggaca aagcgagcat tttctgcaga aacaagatta
     9781 attttggaaa catacgtacc actttgaggt atcgtataca cgagttcttg ctgacgtagt
     9841 tgaatcaatg cttcgcgaat gggagtccgg ccgatagcaa gatgttcctc cagtgccttt
     9901 tcagatattt tccttccagg ttctaagtca gcatagataa tttgtttacg aatcgcttga
     9961 taagcttgtg attgcaagct gcttttcatc tttattcctc caaaatcatt tctatgaacg
    10021 tagtataaca gaagaggtct gaaaaacaat ctggtatgtc acattaacta gtaaatttgt
    10081 ataaatagta aaattttggt tgacatagcg atttttattg cataaaattc acttgaatac
    10141 aaaaactaat atgttagttt aggaggggaa aaatgattga tttaaacaac tggaggaact
    10201 ttatccctga attatctcaa aaagaagtag aaatacctca ttttgaccgt gcatcattaa
    10261 gagaaaatgg acaaaaaaat cctgtctgga tccatttcgg tggcggaaat ctttatcgag
    10321 gatttcatgc agaaatcgct caacgtttag cggatctagg cgaattgtcc actggtgtca
    10381 ttgtttgtga aacattcgat gaagaaataa ttgaaagggc ttaccaagat tttgatgatt
    10441 tgattcttga agtggtcatg ccaagcagtg gagagttcgt caaacgtata ttagctgcga
    10501 cagctgacag tctgtattgt catccagatt gttctgaaca ctttgataag atgattcgac
    10561 actttgaaaa tcctagttta caattagtta cttttgctat tactgaaaaa ggttatcgat
    10621 tgaaagatac aaataataaa tatataggac cgattgcagt cgatataaaa aatggtccaa
    10681 agaagccatc acatacaatg agtattcttg ctgtgctact ttataaacgt tttttatctg
    10741 gagcttggcc gattgcactt gtctctacag ataatttttc acaaaacggt agtcaattac
    10801 gtacttcttt gctaacgatc gcaaaagaat ggcaaaagga aggttatgta caaaatacat
    10861 ttatccaata cttatcgaat gaagaaattg ttgcgtttcc atggacgatg attgatcgta
    10921 tcaccccgaa tccttccttg tcagttagca aaatgttggc aaaagagggc ttttcaaata
    10981 tgtcgatttt ccaaaccgca aaaggaaata atattgctcc atttgtcaat acagaggaca
    11041 tccattattt aatcatggaa gatcattttc ccaatggacg tccgaaattc gaaaaagctg
    11101 gggtattctt gactgatcgt gcaacggtag ataaagcaga tacgatgaag gtgacgactt
    11161 gccttaatcc tttgcatacg tctttagcta ttatgggttg tttactgggg tataggagca
    11221 tagctgagga gatgaaagat ccagatttat cgctgctagt ttctgaaatc gggtacaatg
    11281 aaggcctccc agttgtggag tctccaggta tcattgatcc aaagatcttt ctggaagaag
    11341 taattactgt gcgcttttct aatccaaata ttcctaatat gcctcagcga atcgccacgg
    11401 atacctctca aaaggtggcg attcgttatg gtgagacgat taaaaaatat atggaaatag
    11461 aagttaaaga cccgcagaca cttgtattca taccattgac tctcgctgct tggcttcgat
    11521 accttattgg cattgatgat caaggaaaac cattcatccc gagtccagac ccactattga
    11581 cagaccttca gcataagctt tccgatattc aactgggtac gcaagataca tcacatattc
    11641 atgagaaaat ggctcctatt ttgtcaaata aagaaatttt tggtgtagat ctttaccaag
    11701 cagagcttgg cagaaaaata gaagaaatgt tcatcaagat ggtagcagga ccaaatgccg
    11761 tacgaaaaac attacaggat tacttacaag aacatggagg aaatttatca tggaaatgac
    11821 ttttagatgg tacggatcac aggaagaaaa gattacctta gatgaaatca gacagattcc
    11881 tgcggtcaaa gggattgtag gcacattgat ggatatacca gtaggagagg tatggcctag
    11941 ggagaggatc aaagaactaa aagaagaaat tgagtctcgt gggttaacac taaaagtaat
    12001 tgaaagcgtc aatgtgcatg atgccattaa aattggatta ccttcccgtg agcagtatat
    12061 cgaaaactat aagcagacaa tccgaaactt ggcagaatat ggaatagaag tcatctgcta
    12121 taattttatg cctgtatttg actgggtgaa atctgacctt gactaccgct tagaagacgg
    12181 ctcatccacg ttagctttta tatctgatga tattccagat gatccaagag aaatcgtcgc
    12241 gcgcatcgaa cattccagca atgaatttgg attgccggga tgggaacctg agcgcttagc
    12301 tcctatcaaa tccttgcttg aagcctatgc atcagtagac gaagaaaaac ttcgagaaaa
    12361 ttttgcctat tttctccaat caatcatccc aacatgtgaa gaggtagggg tcaaaatggc
    12421 tgtacatcca gatgatccgc catactcgat ttttggatta gctcgaatcg taaagaatcg
    12481 agaagattta gattggttgt gtcgcatagt agacagccct tataacggaa tcactctttg
    12541 tacagggagt attgcagaag atccagaaaa tgatgtatat gggattttag cagaattttg
    12601 ccaaagagat cgaatcccat ttgcacatgt tcgaaatatt aaatttttag ctaatcgaga
    12661 tttctatgag gcaccacata aatccgaata cggttcatta gacatgtacc gcatactaaa
    12721 aacaatgtat gacaacggat ttaacggata catccgtcct gatcatggaa gaatgatctg
    12781 gaatgagaca ggaagaccag gatacggctt atatgatcga gcattaggtg ctgtttatct
    12841 aaatggatta tgggaagcaa tcgaaaaagg aaacaaaagt aaagataatt agtgaaaaaa
    12901 actgtaattt tattatgtaa ataagaagga tgttcaaatt cttgatacat aaatccggag
    12961 gttgttaata agcattcgga tttatgtatc ttatttggtc taggaagtaa cttgattttc
    13021 ttttttcgat tgatccatga gtgcttcttc ttttaattca aagatatatt ctgagatcct
    13081 cataatcact tttttccagt cttctggtgt gatgattcct tttaaaatat agattttttt
    13141 gatatgagaa ggcaattgtt tcaaagggca gtcgctgata acgatgttgc agttctccat
    13201 aaaatcattt tgtattttga tcgattcatt tgaaaaaatt tgttctatct taacgatcag
    13261 atctttactg atataaaaat cacgcgtata gttcacacca atcacaattc gcggctgttt
    13321 tttgtagcga tcatacaaat gtactaggtc ttcaaccatc cattgcacat tttccttttc
    13381 taaaataact ggtaacaagt gttgattttg ttctctgaaa tccatataaa attgttcaat
    13441 ggcggtttgg acttctttca atacgctatg ttcttgataa agcatgaaag taggaagggg
    13501 cgtatttggt tgattgaagt taagaaatcc attataaagt ttatttcgtg cgaatgccaa
    13561 tgagtaaaga atcttttctt tttcaggtat aaacaaatca aatacagtac aaagggactc
    13621 caccatttct cttgaataaa gaacatgagg aacgtttgct ttttttaaaa gtttgtattg
    13681 ctccaagctt gtttcttccg attcgatttt cgtgatagaa agtcgagcta aaatattcac
    13741 taagatgcgt tgttcgcgac aaattggcag attaggtcgc aaaatatcta cagagagatc
    13801 aataaaaaag tcaatatatt catactctgt gaattctttt tgtacgtctt tcacagaagt
    13861 tgcaggaaaa ttctctttac atatttttaa tagaaaatat aatttatcta tcataccgct
    13921 atttagtcgg tcaagtactt cttttttaaa aaaagtattc atcataaaag ccactacttg
    13981 cggatcttca tcataaaatt ctgtagcagt acttgaaaag atgttccaat aaatatctaa
    14041 tgctgcatat tgaatttgta cctcttttcc agaaaaagac tttctgccat tattagaaaa
    14101 ggagatagac aaaccgtact atgctaaaaa tgcattaatc tttttcattt tcgtatacaa
    14161 ataagaggca ctatagtttg tttgctgact gaaactgata atcgaatttg tctgacagct
    14221 gaacaaagaa gtcaagatct gataactgac tgattcgttg cagtatcttc caaatagacg
    14281 ataatagcag taagccggat caattccttc aaaattcaac tttaaaaaag gtttttgctt
    14341 atcaatatgc agttctgtta gattgagacg ctcaaag
//
LOCUS       DACDPS010000060        14084 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.060, whole genome shotgun sequence.
ACCESSION   DACDPS010000060 DACDPS010000000
VERSION     DACDPS010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.060"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..3649)
                     /gene="rpoC"
                     /locus_tag="IWJ07_001950"
     CDS             complement(<1..3649)
                     /gene="rpoC"
                     /locus_tag="IWJ07_001950"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAP9333524.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQT"
     gene            complement(3728..7345)
                     /gene="rpoB"
                     /locus_tag="IWJ07_001951"
     CDS             complement(3728..7345)
                     /gene="rpoB"
                     /locus_tag="IWJ07_001951"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP9333525.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8073..9041
                     /locus_tag="IWJ07_001952"
     CDS             8073..9041
                     /locus_tag="IWJ07_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9333526.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9059..9964
                     /locus_tag="IWJ07_001953"
     CDS             9059..9964
                     /locus_tag="IWJ07_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9333527.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            9961..10767
                     /locus_tag="IWJ07_001954"
     CDS             9961..10767
                     /locus_tag="IWJ07_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9333528.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            10924..11892
                     /locus_tag="IWJ07_001955"
     CDS             10924..11892
                     /locus_tag="IWJ07_001955"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAP9333529.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12209..12733)
                     /locus_tag="IWJ07_001956"
     CDS             complement(12209..12733)
                     /locus_tag="IWJ07_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAP9333530.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13020..>14084
                     /gene="scm"
                     /locus_tag="IWJ07_001957"
     CDS             13020..>14084
                     /gene="scm"
                     /locus_tag="IWJ07_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP9333531.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT         3982 a         3040 c         2787 g         4275 t
ORIGIN      
        1 ttgtttgatt gttcattgcg tcttctgcag ccatttgcgc ttcaatatct gaaatactgt
       61 agacattttc gcttgctaca ccgacttctt ttggttccat gtcacgataa cgttgcatac
      121 cagtaccagc tgggatgatc ttacctataa taacattttc tttcaaacca agaagaggat
      181 ctttcttacc gcggatcgca gcatccgtca agacacgagt tgtttcttgg aatgatgcag
      241 cagataagaa actatttgtt tctaatgatg cttttgtgat acctagtaag acaggtcgag
      301 aagttgcagg aacgcctcct gagactagcg tattgtagtt ttgatcttta aagtctgcga
      361 tgtctaataa tgtacctggt aagatatctg tatcacctgg atccattaca cgaactttac
      421 gcagcatttg acgaaccatt acttcgatat gcttgtcgcc gatttctacc ccttgcatac
      481 ggtaaacacg ttgtacttca cgaagcaagt agttttcaac agataataca tcgcgaactt
      541 gcaacaattg ttttggatcg attgatcctt ctgtcaatgg tgcaccacgg tggatcatat
      601 cgccttctgc tactttcata cgtgctgtat aaggaacagt atacgtacgt gtatctgttt
      661 ttcctttgat tgttacttct ttggtacgtg ttgcaggatc ttctgagata tcgatgacat
      721 cccctgttac ttctgtgatc actgcttgcc ctttcggatt acgtgcttca aagatttctt
      781 ggacacgagg aagaccttga gtgatatcgt ctccggcaac cccacctgta tggaatgtac
      841 gcatcgtcaa ctgagtacca ggttcaccga ttgattgagc ggcgatcgta ccaactgctt
      901 ctccgacttc aacgtcagag ccagttgcaa ggttgcgtcc gtaacaatgt ttgcagacac
      961 catgttttgt attacatgtg aagacagaac ggattgtcac tgtttcgaca cctgcatcaa
     1021 cgatttgttt tgcgatgtct tcggaaatca attggtttcc tgcaatgatc aattcatttg
     1081 tttcaggatg acgtacttct ttacgggtgt aacgtcccac taaacgatct tctaatgatt
     1141 cgatgatttc atttccctcg cggatggctt ggatttcaag acctcggtca gtaccgcaat
     1201 cttcttcacg gatgattaca tcttgggcca cgtcaaccag acgacgagtc aagtaacctg
     1261 aatcggcagt cttcaaggct gtatcggtca tccctttacg agccccgtga gttgagataa
     1321 acatttccaa gacggatagt ccttcacgga agttcgaaat gattggcaat tccatgattc
     1381 gaccattagg agcggccatc aaaccacgca ttccggcaag ctgtgtaaag ttggaaatgt
     1441 taccacgagc tccagaatca gacatcatga agattggatt tttcgcttcc aaaccttcca
     1501 tcagtttttg ttggatgctg tcttttgcag cattccaaac agcaatgaca cgttcataac
     1561 gttcgtcatc tgtgatcaga ccacgacgga attgttttgt aatagtttca acttgtttat
     1621 gggcattttc aatgatttct tgtttttcat tcagtacact gatgtcagcg atccctactg
     1681 tgataccggc atgtgtcgaa tgtttgtacc ctaagtcttt catacggtca agcatcttag
     1741 aagtttctgt tactttgaat cgtttgaaga cttctgcgat gatgtttcct aagtttttct
     1801 tcttgaacgg taatactaat tcttgctctt tgatatgtgc aggaatatca gtaccagctt
     1861 caacaaagta tttgtccggt gtttggacgg tcaagttgaa gtctgttggt tcattcagat
     1921 aagggaattc aggaggcatg atctcgttga agatgatctt accaactgtt gtgatcatcg
     1981 tacgttcttt ttgccattca gtaaatggtt tttcacctaa tgagttaggg ttcacaccga
     2041 tacgtgaatg taaatgaaca tatccgttgc gccatgcgat aacggcttcg ttttgatctc
     2101 tgaagaccat tccttcgcct tcgcgtcctt cttcttccat tgtcaagtag tagttaccta
     2161 gaaccatatc ttgggaagga gtaacaactg gtttaccatc tttagggttc aagatgtttt
     2221 gagcagctaa catcaacata cgtgcttctg cttgtgcttc ttcattcaat ggaacgtgga
     2281 cagccatttg gtctccatcg aaatcggcat tataagcttc acatacaagt ggatgcaaac
     2341 ggattgcgcg tccttgtact aaaactggtt cgaatgcttg gataccaagt cggtgaagcg
     2401 taggtgcccg gttcagcaat actgggtgtt ccttgattac gtcttctaaa acatcccata
     2461 cttcgtcttc ttgacgttcg attttacgtt tcgcattttt gatgtttgaa gctagttcgc
     2521 gttgaacaag ctcacgcatc acgaatggtt tgaataattc aatggccatt tcttttggaa
     2581 gtccacattg gtacatcttc aagaaaggtc caacaacgat aacggaacga cctgagtagt
     2641 cgacacgttt acctagcaag ttttgacgga aacgaccttg tttccctttc aacatatgag
     2701 aaagagattt caatgggcgg ttacctggtc cagtaactgg acgtccgcga cgaccgttat
     2761 cgatcaatgc atctactgct tcttgcagca tacgtttttc attttgaacg atgattccag
     2821 gtgcattcaa gtctaacaaa cgtttcaaac ggttattacg gttgattaca cgacggtaaa
     2881 gatcattcaa gtcacttgtt gcaaaacgtc caccttctaa ttgaaccatt gggcgtaaat
     2941 ctggtggaat aacagggata acgtccataa ccatccagct tggttgattt cctgaagcgc
     3001 ggaatgcttc taaaatatcc aaacggcgga ttgcacgtgt acgtttttgt ccttgtgcag
     3061 atttcaattc ttctttcaat tcggctgctt ctgcatctag atcaacatta tcaagtaatt
     3121 gtttaatcgc ttctgcaccc atagcagctt ggaattcttg tccatattgt tcacgttttt
     3181 cgcgatattc gcgttctgtc aacaattgtt ttttctctaa attcgtattt cctggttcga
     3241 tgactacata tgaagcaaaa taaatgattt cttccaaagc gcgtgggctc atgtctaata
     3301 caagacccat acgtgaagga atccctttaa agtaccagat atgtgaaaca ggagctgcta
     3361 attcaatatg tcccatgcgt tcacggcgaa ctttcgaacg agtaacttcc actccgcatc
     3421 gatcacaaac gattccttta taacggatac gtttgtattt tccgcaggca cattcccagt
     3481 cttttgtagg accaaaaatc cgctcacaga acaacccttc gcgttcaggt tttaaggtac
     3541 gatagttaat cgtctcaggt ttttttactt caccgtaaga ccagcttctg attttttcgg
     3601 gagaagccaa acctatttgc atactttcga atttatttac atcgatcaaa aggggttccc
     3661 tccatttcat ttaatggcaa accgcaagac ggctgatctt cgaccgcctt gcagctcata
     3721 gaccggatta gtctaaatta tcttctgcgg tttcaaagtt ttggatgaca tcgtcttttt
     3781 catcttccac ttgttcagca gctttctttt ctaattcttt ggcagtttgc tgttcggcaa
     3841 attttgtcaa agcatcaaca gtgatcaaat cgtcgtcttc atcgtccatg tcgcgaagtt
     3901 cgatctctgt ttcttcgata tccaagacac gcatgtccag acctagtgat tgcaattctt
     3961 tcaccaatac gcggaatgat tccggtacac ctggttttgg aattggttca cccttgacga
     4021 ttgcttcgta tgttttcaca cgtccgacta cgtcatctga tttgtatgtc aagatttctt
     4081 gtaaagtata agccgcaccg taagcttcca gtgcccatac ttccatttca ccaaaacgtt
     4141 gtccaccaaa ttgagcttta cctcccaatg gttgttgtgt aaccaatgag taaggtccga
     4201 ttgaacgtgc atgcaatttg tcatcgacca tgtgagcaag tttgatcatg tacatcacac
     4261 cgacagacac acgtccatca aatggttcac ctgtacgtcc gtcgtacaga acagttttcg
     4321 catcgctggc cataccggct tcacgaactg tttcccatac gtcttcatca cttgcaccat
     4381 caaatactgg cgttgctacg tggataccta actgacgtgc agccataccc aagtgtaatt
     4441 ccaatacttg cccgatgttc atacgtgaag gcacccctaa aggattcagc atgatatcga
     4501 ttggtgttcc atcaggtagg aatggcatat cttcttccgg cataatacgg gatacaaccc
     4561 ctttatttcc gtgacgaccg gccattttat ccccttcatg gattttacgt ttttgaacga
     4621 tataaacacg aactaacatg tttacgcctg gtgacaattc atctccagct tcacgagtaa
     4681 agattttcac atcgtgaacg ataccgccgc cgccatgtgg tacacgtaat gatgtatcgc
     4741 ggacttcacg agctttttcg ccaaagatcg catgcaatag acgttcttct gcagataatt
     4801 ctgttacccc ttttggcgtg actttcccta caagcaaatc gccatcttgg acttctgcac
     4861 caatacggat gatacccatt tcgtctaaat cttttaatgc gtcttccccg acattaggga
     4921 tttcgcgtgt gatttcttca ggtcctaatt ttgtatcgcg ggcttctgat tcatattctt
     4981 caatatggat agaagtgtat acgtcatctt ttaccaaacg acggctcatg atgatcgcat
     5041 cttcatagtt gtacccttcc caagtcatga aaccaactaa aacgttttgg cctagagcca
     5101 tttcgccttg ttccatagat ggtccgtctg ccagagtatc ccctttttcg actttctcac
     5161 ccaaatgaac gattggacgt tggttgtagc ttgttccgga gtttgaacga cggaattttg
     5221 ttacatcgta tttatctaat gcaccgttgt cgcggcgtac gcggatttca gaagcatcaa
     5281 cgtattcaac gacaccgtca tgtttacata acaaagcagc tcctgagtca tgcgctgatt
     5341 tgtattccat accagtacca acccatggtg cttgaggatt gatcaacggc actgcttgac
     5401 gctgcatgtt ggcacccatc aaggcacggt tggagtcatc gttttccaag aaaggaatac
     5461 atgctgtcgc aacggcaact acttgtttag gtgatacgtc catgtagtct actttatcaa
     5521 tagatacttc taagttttca ctttgagcac gagccataac aacgtcttga gcaaatgttc
     5581 catcttcatt caatgggctg tttgcttggg ctacgatgta atggtcttcg atatcagctg
     5641 tcaagtagtc gatttggtca gtaacacgac ctgtttcacg gtctacacga cgataaggtg
     5701 tttcgataaa tccgaattta tttactttcg cataactgga caaactattg atcaacccga
     5761 tgtttgggcc ttcaggagtt tcgatcggac acatacggcc atagtgggag tagtgaacgt
     5821 cacgaacttc ataaccggcg cggtcacgag tcaatccgcc aggtcctaag gctgaaagac
     5881 gacgtttgtg tgtcaattcg ccaagagggt ttgtttgatc catgaactgt gacaactgag
     5941 aagaaccaaa gaattctttg atacttgcca caactggacg gatattgatc aattgttgag
     6001 gtgtcaatgt ttcagtatct tggatcgaca ttctttcacg aaccacacgt tccatacgtg
     6061 ctagcccgat acggaattgg ttttgtaaaa gttcaccgac tgaacggata cgacgatttc
     6121 ctaagtggtc gatatcatcg acattgccga taccttccat caagttcaag aaatagctca
     6181 ttgaagcaat gatgtcggct ggacgaaccg tttttacagc tgcttctgga tagccgtttc
     6241 cgatcacgtt tacttcacgt tcagggtctc ttggtgaaaa tactttgatg acttggacag
     6301 tcattggatc tgtgaccaca ccgtcttctg atggatagta agtcactgaa tttaggccat
     6361 tatcaatgaa aggagctagt gtttccatga cttggtgagt cagaactgtt cctttttcaa
     6421 cgatgatctc gcctgtttct ggatcaacta atgtttcagc taatgtcaga ttcaatagac
     6481 gtgttttcaa atctaatttt ttgtttactt tgtaacgacc aacgtttgct aagtcgtaac
     6541 gttttggatc gaagaaacga gcattcagca agttacgtga gctgtctgct gtttttggtt
     6601 cgcctggacg caaacgttcg taaacgtctt tcaaaccttc ttctgttctt gagtcgcttg
     6661 cgtttttatg caagtctttt tcgactgtat tgcgtaatgt ttcgctgtcg ccgaagattt
     6721 caaagatcgt atcatcggaa ccaaatccta acgcacgaac taatacggtt aaaggaatct
     6781 tacgtgtacg gtcgatacgt acataagaga tgtcttttgc atcagtttcc atttctagcc
     6841 aagctccacg gttagggata acagtagaac cgaatccttc ttttccgttt ttgtcgactt
     6901 ttccatggaa atagacacct ggtgaacgga ctaactgaga aacgattact cgctctgccc
     6961 cgttgatgat gaatgttcct tgttctgtca ttaatgggaa atcaccgaag aatacttctt
     7021 gtgctttgat ttcgccagtc tcgcggtttg tcaaacgcaa tgttacatgt aatggtgcag
     7081 agtagtttgc atcatgcgca cgagcttctg ctactgtata ctttggttct ttcaattcat
     7141 aatctacaaa ttccaaagat agattgccat tgaagtcatc gattggcaaa atgtcttcaa
     7201 acatttctct aagtccctca tctaagaacc attggtaaga gtctgtttga atttcgatca
     7261 aatttggtaa ttccaacact tcactgattc ttgcgaaact tctacgttcg cgatgttttc
     7321 cgtattttac tacgtgtcca gccaagctct tcacccctct aaaatgtcat tcaaaaagac
     7381 tacccgatca attgttacaa taatatttca aatattattt ttaggtaaca acagacattt
     7441 tttgcggctt ttaggcaaaa aaaaaccaaa aatagaacat tctcttgaaa ccttacttcg
     7501 tgagcttcta cttttgtttc cttatattaa agccgaaacg gacaatattt cgtttctgcc
     7561 ttttcttcat cagatagttt ttgcaacttc atatactaca cttattttct ataaaagtca
     7621 acacttcctg gcgtttcttc tgctttttcg caacattttc ttaatgtttt caattttatg
     7681 ttttcaatta tctgacaatt cttaattgac agaccttttt atctcggtta tactttagaa
     7741 aattcatttt gtggaggaac ttctatgcga accaacatca accaaatcgc accaacacaa
     7801 ctgaataact tttactttag ctactttaga taggtttgta ttcatcatga tggatacaaa
     7861 cgttaaacga cagtttgtat ctatgatggc taaagaactt tgtattgcag caaaaagcca
     7921 cgtagacaaa atgctaagtg gctgagcagt gaaaatacaa agtaaaagta tcaagcattc
     7981 tactttttca atttattttc cgacttttca atccatttgg acattttgtc tgaatggatt
     8041 ttttgtttcc caaaattaag gaggaaaaag aaatgaaaaa aaatataatc atcggattag
     8101 gcgctgccct gctactagct ttaggaggtt gcgcacaaaa agacaattcc gcagaggcat
     8161 cagatggaca aaaaacaatt ggcgtattgc aacttgtaca acataactcg ttagatgctg
     8221 cctataaagg attcaaagaa ggattagcag aagaagggta taaagaaggc gataatctaa
     8281 caatcgatta tcaaaatgcc caaaacaatc aagacaacct gaaaagtatg agtgagaaac
     8341 tcgtcaaaga agatcctgat ttgctacttg gaatcgccac tccagcagca caatctttat
     8401 taaatgaaac aaccactatc ccgatgactg tcacagcggt cacagacttg gaacaagccc
     8461 atttagtcac atccaatgaa aaacctggcg gcaatgtgac tggcacgaca gatatggtgc
     8521 caatcgaaaa acaaatcggc ttactgttaa atatcgtgcc agacgccaaa acaatcggca
     8581 tcatgtataa caacggtgaa gcaaactcta agatccaagc tgatttggca gaaaaagaat
     8641 tgaaaaaagc gggcgtcaaa gtcaaagtac tgacagcaaa ttccacaaat gatgtccaac
     8701 aagtgacgaa aagtctagct aaagatgtag atggaatcta tattcctact gataacacgt
     8761 tcgcaagtgc ggcaagtgtt gtcggtgaag tcgcaaaaga aacaaaaaca cctgtcgttg
     8821 ctggcgcagt tgatcaagtc aaagacggcg gtctagcaac agtaggaatc gactacgaaa
     8881 gcttgggtaa acaaacaggg atcatggcag caaaaatctt agacggcaag aatccaagtg
     8941 aacttcctgt cgaaacagcg aatgatttgg aactgtatgt caataaagac atggccaaag
     9001 cattaggcat caatccagac agcatcaaag aagctgaata aaacacagga ggattctcat
     9061 gacaaatttg ctctctcttt tcttatcatc tgcatcgcaa ggggtcttat gggccctact
     9121 tgcgattggt gtatacgtca ctttccgtat cttggacatt gccgacttga ctgctgaagg
     9181 aagctttcct ttaggtgcag ggatcgcggc agtaagtatt acaaatggct atcatccatt
     9241 gacagcttgt cttctaggct ttttaggggg tgcggcagca gggcttgttt ccggactgct
     9301 tcacacaaaa ttgaaaattc ccgcccttct tgcagggatc atcaccatga ctggtttata
     9361 ttctgtcaca tcccgtgtga tgggtgcgcc gaacatctct ttattgggtc agccaacgat
     9421 ctttcattgg gtcgagtcat tcggcataac aaaagacaac gctgtactga tcgtcggtct
     9481 gctttttgct ttgatcgttg tatgtttact tgttttgttc ttacgtacag aaacaggact
     9541 tgctattcgt gcaactggcg ataatctggc aatgagcgaa gcaaacggga tcaacacaga
     9601 taatatgaag atcatcggtt atatgatttc taatggatgc attgctcttt ctggctcctt
     9661 gcttgcgcaa aacaacggct ttgctgattt aaattctggt attggaacga tcgtaattgg
     9721 acttgcttct attatcatcg cagaagtttt gttacgtaac cagccactat ttctccgtct
     9781 cttgactgtc gttttaggtg cggtcattta tcgttttatt ttagcgttag tcttcgaact
     9841 gaatgttgaa ccaagtgatt cgaaacttgc ttctgctttc gtgctagtga tctgtctttc
     9901 tttcccgcag atccagcaaa aattccattt acctcaaaaa aagaaaggcg gtagtgtcaa
     9961 atgaatgacg tcttaacatt ggaaaatctc catcatacct ttgaaaaagg aacgatcaac
    10021 gaaaatcatg tcctgaaagg cattaatctt tccttagatc ccggcgactt cgtgacgatc
    10081 atcggtggaa atggggctgg taaatctact ttgctgaaca gcatcgccgg tacattttct
    10141 gtcgatcagg gtcaaatttt attgaatgga aaaaatatca cgaaaaaatc tgttgtcgaa
    10201 cgttcgaaat ccatcagtcg cgtattccaa gatcctaaac tagggacagc cgttcgttta
    10261 acggtagaag aaaatcttgc tcttgctatg aaacgaggaa aaaaacgcgg cttcttccgt
    10321 ggtgtcaaac cacaagaccg aacatttttc aaagaccagc tggttcggct aaatttgggg
    10381 ttggaaaatc gtttatcctc agaaatcggt ttgctgtctg gaggacaacg tcaagctatt
    10441 acgttattga tggccacatt acagcgtcca gaactgatct tattagatga acacactgca
    10501 gcgcttgacc ctgctacttc caaaacggtg atggaattga cagaaaagct gatcactgag
    10561 cagaatctca ctgcttttat ggtcactcat gacatggaag acgcgatccg ctatgggagt
    10621 cgactgatca tgctgcatca aggaaggatc gtcgtagata tccagcaaga ggaaaaacag
    10681 cagctcacag tgccccaatt attagcgttg ttcaaacaaa acagcggcac ggcattaaaa
    10741 gacgatcaag tacttttatc ttcataatca gaaagaggat gggacaaaaa atgaacgccc
    10801 accctcttct tttttctctt tccttctttc gtttttgctt tatgtatcaa ccgttgtttc
    10861 ttcgaaaact attttgttct ctccttcttc ctgatagaat ggatgggaaa ggagcttgat
    10921 gccatgacaa cgaaagacaa ggtacttcaa ttattgaaaa agacaccaga ttttctttcc
    10981 ggagaaaagt tggcacagca actggaagtt tcccggacaa gtatttggaa agccatcaaa
    11041 gaattggaaa aggcaggcta tcgttttgag cacgggccta cagggtatcg ttacctgccc
    11101 tctgacgtac tagatgcaaa agaaatccag caagagctac ggtcgtttat tcctgatttg
    11161 aaagtacaaa cgatgttgac ttctgaatct acgatgaaag atgccaaact ggcagcagct
    11221 caaggagatt ctgtttcgaa gttatttgtt gctgatacac aagaacaagc tcgtggacgt
    11281 tttggacggc cttttttcgc tgaacctgga cgaggaatct acatgagtct cttgctccat
    11341 ccagacaaac ggtttgatga attgcctcaa tatacgatca tcgcagccag tgcctgtgtg
    11401 aaagccattg aatctgtaac cggtcgttct gcttcgatca aatgggtcaa tgatatctac
    11461 atggatggaa aaaaagtctg tgggatttta tctgaagcag tcagtgatat ggaaagcggc
    11521 aggatcagtc atatcatcat tgggatcggg cttaattttt ctgttcctca aaatcatttt
    11581 ccagctgatc tccaagaaaa agccatctct gtctttcccg acggacaagc tagtgtcaca
    11641 aggaatcagc taatcagcaa aatctggcaa gaattgttca cttatctttc tcacccagaa
    11701 aaagcgttgg acttctataa agaaaaatca ttcgtattgg gaaaaaccgt gtcgttcaat
    11761 caaaaaggaa cgacttatgt cggtcttgca aaagaaatcg gtgatcatgg cgaactgatc
    11821 gtccaaacaa atgaacagaa gatgagtttg tcttctggcg aaatcagtct cacatctatc
    11881 cgtacgcaat aattatcaga tttcacaaaa ggacacctga tctcatacag tgtccttttt
    11941 tctgaattct cttcaaaaga ctctccttat tctatcagct gacagtttct ctatcctcta
    12001 gaaaaaggta ttctaaaaat atctataaag catggtacac tttctttaaa tgaatcaatg
    12061 caaaaaacaa gagagtcatc agtttacacg atcaaaagag atagcaggcc gcttcctttt
    12121 tcaatcttat agatagacca tgggtaacga taaaaacaaa aaagaacttg cgacttattt
    12181 tgccgtaagt tcttttttag ctagtgcctt aatctcttaa tcccaacaaa cgtctcgcat
    12241 agatcacctg accagagtgc atagcagcat catcaataat agaaacgaga cgcattcctc
    12301 gtgtgactgg tggtgtccag ttctcgtcaa ttacttcatc tagctcggaa ggttccagcc
    12361 ttcttaagta agaaatagca aaatcagcag ctcctcgcaa gtatcctagc agatcttcta
    12421 tttgattagc ttggatctgc tttgcttgtt ccagactatg cttccaatct tgttcgttat
    12481 ctgctatcgt catcggaaac cggctttccc agtcttgact atgccaaatt ggagaagttc
    12541 ctgctaaatc agcaatctgg aaatctaatt ctcgagcggt atgccaagcc agccaagtca
    12601 ttgatttgat ctgcggggct ctatcttcca ctggaaaacg attggcctcc tcaatagaga
    12661 cgccttcaaa caaacgttca aaccgatcgt atgcccgatc cacactctcg catagcatat
    12721 ctaaataatc catttatttt cctccttttt atttatccta cttacttttt cctaattcta
    12781 ggatacctac ttatatgtta ctacacaccc ttatttttta cactttaaca gtcaccatca
    12841 tcaatagttt ggagattgaa agaaaaagat agtgtcgaac tatcaattga acaagaagca
    12901 acaatatgaa cgtttcatta atattcttta aataaacaat gatttttcaa acgaacagtt
    12961 taagactagg agaaaaagag tataatagga aaatgtttga aaggaaagga aggggaaaaa
    13021 tgaaaaaaag ggcaagttta ctagtcctag ttgctttgtt gttcaatatc ttttcgtatg
    13081 gtgtggccgt ttcagcggaa acatacattc ctaaagaccc tgggaatatt tataatgatc
    13141 taactggtaa ctatggcttg ttaggaattg ctagtcaatt ccacgtattt gctaaaaata
    13201 agacaactgt gaatgcacat acagacggaa atatagcaac acatgaactt gatgcaaaaa
    13261 ataattttgg tacagagctt catgaaggtt tattgaacca agaaataaat tatatacaaa
    13321 aaactgattc tttcgctaat tcttcaggca tacctacagg tagcacaaga atgaataaat
    13381 ttgtcgttgg cagtaacgtg gatgtagcac ttcaagatgg tcatcctaca gtcaatggat
    13441 ccaggcttga tcacttaact ccagatgatt tttataaaga tcgtgctggg catgaatata
    13501 ttgatttcga gcaggaattt gctaaattga atgtagcagc aaatgatctt gcaacgatca
    13561 ctccagctaa aacatatact gctgcagatt ttccagacat gaacaatcgg acaatcgatt
    13621 taactgattt atcagatacc gggtttttat tggtaaatat cgatgcagaa gtgttaacga
    13681 tgaatacgcc acttcaaatc attaatccaa atgatcaagt agttgtattt aatgtcatca
    13741 acagttcttc tgcattgaac gtacagtcac caatcaaata caatgatcgt tcaaatcatg
    13801 aaacagaaga tttttcggat gcgaatatct cttggaattt tggaaatgag atgactgatt
    13861 tgacaatcag tgctcctttt caaggaacaa tccttgcgcc taatgctact atccgcatta
    13921 ctcaaaacca agacggaacg attataggaa caaatgtgat tttagatgca gctaccaatc
    13981 gttgggatcc taacgaaata tttatcagcg acaatggtac agatacgaca gacacaagcg
    14041 acagtacaga tacatctacc acaagtgata gctcagattc atca
//
LOCUS       DACDPS010000061        13992 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.061, whole genome shotgun sequence.
ACCESSION   DACDPS010000061 DACDPS010000000
VERSION     DACDPS010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.061"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(269..2443)
                     /locus_tag="IWJ07_001958"
     CDS             complement(269..2443)
                     /locus_tag="IWJ07_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HAP9333532.1"
                     /translation="MMNAPKRPTHFEQMFSVDRKRKQEKETPKSRFQQLYFIVTLLGV
                     CLYPFFIVGSIPLAIVFMIDKKETRAHIYDFEYESFLKRRSSLFFIFALGFTLLNLFC
                     FIFVLPRGYLSCYLLFPLNLFSHALQFNGQTIFALLLGGTGAGSCLIAYASFIEKRKV
                     VSKKERQTALLNSKEYKERRKNKFQESQKYTAEYQQAYQVAIVIKDVALRNKQLASLA
                     KVILLGTDEYGLPYVMNFSELNQHVIIPATTGSGKTTVIQLLAEHACKFRIPVILVDG
                     KGAGETLQALKEVAEAYNRPIEVFVDGGDLRYNPVKNGNDIAIRDKLTNLAETESVYY
                     STAAKALLQVTIQLLDEFQKSHKIERNLVWLQHYLLPRNVLHLFADRIQLKNPDLFKE
                     KVVVEKKKTTKKKTKKNEAMDKTERDFADVEQLSEERIVEHDRKTVDLETYYLLLKEN
                     ISLMTEIEQTLFKRLFVRYEHKKNPFYLYATSENLQTNINMLLDSELGELFDTSGGKR
                     ELDIQEVVKEGKIVYISLNGLIYSEFIRTLAQMLVGDINFFASEVYKQAGETANRSKV
                     LVIFDEPASYLNDNLIDLVNKGRGAGVHAAYAPQTLADIDRIDPVLRKQLIGNVNTFF
                     IGKTNDPTEIDYWADLMGTEEDIELTEMTTQEAGYSDMGKVDWTGERGTKRDVDRFKF
                     NPNRIRALRTGEFVVYRTAEDVQEPPRVVYIRKPNLEKQYAK"
     gene            complement(2459..4801)
                     /locus_tag="IWJ07_001959"
     CDS             complement(2459..4801)
                     /locus_tag="IWJ07_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333533.1"
                     /translation="MRKKSLKWWTLLLLGGLFFCLQGATVSADQMNDGAGVTETDRSE
                     GENANDKVEVKEVTTDVDQLYKNYKKNSFELMTNDYESMNLKKKLSYNVAGVMKNFVW
                     SGVKMLGEFNASMVEFLFELDIISAIREPIQNLTQKIADNMLSIAGTIGISFSAIMMA
                     IKYAGEARMKQALRVFLMTILIFTGLSVLKDSGTNKNLFDVLFSLDSTVETAFVKVNP
                     VLTPEEETNYETKETEEVTADKKVETAGKLMAARVFYSNVYEPYLLLNYGTTNVDTIR
                     EKKVKYAGKEYDRINLLLDNDLGTKIGEKIHDKVTEYEADTLKNKNIQYYNSWKNTAF
                     ALFYFVVNLIQTVVYFVLSMFRLIIGTMQLLLVPMLPFLLFLGLFLVTMNVFGNYAKV
                     FGILTFLKGMTGFACILFSSFLSYGFQLSSTQDDPWEKILLIIVYLFAPLGIYLFRGF
                     LGSLFTGRMGVRGALAFAGNPFGAEAKLRREAKQRLKERKEARKAQKQKEKENARKRQ
                     ERQRKRGGGSDVRMPQTAKQATRSAFRRESAQQPRHKAPNRMERMAQKARDLHDQAKQ
                     AEEQSQERMQRTAMEKQARKTADLLQRELQGNPSLRERSRKGASRSPYRNQGSAMVVS
                     LANPKQKHKSYGQKHTPRTELNDRKRRQGQTSRRRKEALTPVTKTSSMKTRTVAGQKH
                     AVQKPLRKGNVPRATGSTTPKSVRRPEGVREHMSAVNRVTAQTKAPSTVSKAYSTMKQ
                     TKSPKQAPKTRSEFRATQAKMKRKREKQPIYRREMKQRKR"
     gene            complement(4823..7366)
                     /locus_tag="IWJ07_001960"
     CDS             complement(4823..7366)
                     /locus_tag="IWJ07_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HAP9333534.1"
                     /translation="MAFLFLLVVFIIGIWQFKKKKTKLPRLEFPIDAIIRNFVLTKND
                     DVWVGYQLSSQVFPLNDIDFFKNYLQDGEGILGHDEYDYHFINIPRQFNLDTHIEETI
                     RDLVKGECADLGKVYFEQAGKILKDEVQLNEYRTYLFVRFTTEMKVSDPIEYFQLLKH
                     VFKKFIYRITGQRVPISSVLPTYETLEKELYTELSNYKPIERLSAQEIGQIYYYFFHR
                     SEEKLPERQLTPMEICEGVISNEAGYIAIEQLNQTHYSTFLSQTDAPLAMYGSSFIQN
                     LQDSLSFPIDTHIRLRFEHSKSDERKINKMAGRIYEQEKEYETVDGILDSDDVLNFGT
                     ERLMDVKDKLKRKEKRLCYSTVTLVVSASSKEELEERVKDIEFAIESTDFKFYRSVAD
                     QLTLFNQCLIGSTQRFRSYEQVATTGFVADYGLDLKKEVGNKKGFPLGRIVIAKKFKS
                     AKEALALSSKLVWYYPNLTKKSIEGAEHTNGNTLIIGPPGKGKSVLVKYIFLWLMFLG
                     QKILYIDPKNETQKFFLKALEKFGHIPEFVAMYERINFISLSSDESCRGMLDPLLFLP
                     KEEAIQTAKNVLGMLGEVDTNRETASHKKTVIQDAIDVVYTMPGKHSLSQVIEEIRKS
                     DKELAKLILGHSVGLGKILIGNEHSTPIRFDSQINVLGTQGIRIPTQKEIDSGRLNSE
                     QLAGMAIMEVIMKFTYIFSTNKEEDAAIIYDEAKGYEDTAQGSYLIDDSLRKGRANNT
                     DIYLVVQAFKDYDQEDKKELISYKFAFRPKQKEAREKLLSFFDMEVNSANLEMMEQLQ
                     TGTCLFQDHLGRNQPIAIDVLFDSWLLAVSSTENQDEKIQAMLENEKQR"
     gene            complement(7376..7774)
                     /locus_tag="IWJ07_001961"
     CDS             complement(7376..7774)
                     /locus_tag="IWJ07_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9333535.1"
                     /translation="MEYNYTKEFKHPHKIYGIKGQKIPWISNGIRMEQLVIGGVFLFI
                     LLILFLIAYVNQISFLQTLFRKSWLIMIFGIGVLVWTLFSMKWDNKHFFEYLIGRTSY
                     YQKRKQRFEHEMYVPFFHEKVTYSLHKWKE"
     gene            complement(7855..8139)
                     /locus_tag="IWJ07_001962"
     CDS             complement(7855..8139)
                     /locus_tag="IWJ07_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333536.1"
                     /translation="MKLFDLKSLFDDVSGQVIWLLAIVAIVLMVGAYATQGFARAAFA
                     IIGVFILIMLVLLLSNAVDIGEWLKNIIFIKDSEENVGVTQMIVPWIQLS"
     gene            complement(8163..9161)
                     /locus_tag="IWJ07_001963"
     CDS             complement(8163..9161)
                     /locus_tag="IWJ07_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9333537.1"
                     /translation="MSNAKFNPQAFQTKPPKQQKEKKQKKRFLFQKKKTEPSDRMIQF
                     SQKSAMSVFSGAFFCLFLLMGVVLLLNFGRVDTLTRLAVKKQVNPETLLTEVNQTLSS
                     TEQLKYEGQHFIERLFTYSPEKRNEWEASVNHYLAQGLEASDLGFVTTATNRVAKDVR
                     FIKLEEIDLTQAMYRLFYEVTFTEGEHWKSSQLSFPVSYQNNVLKVLDGVQFLNLSES
                     KNTNSVNYQENRFYSSGQAVTESEEIKLEAFVKRFFELYVKNDEKLALISNEKGLEGG
                     ELINVQLDHAVKNKEGNYFLQGKYRFYFEKGNEFQSNFSMEVKTSGDSYFVTTLNE"
     gene            complement(9154..9501)
                     /locus_tag="IWJ07_001964"
     CDS             complement(9154..9501)
                     /locus_tag="IWJ07_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333538.1"
                     /translation="MERQESIREVQEVPTEQAKETQEGRSFSYGKNFSFLRPSIQISV
                     FVLWRIHNNRYSVGSQLFYQEICHALSKKYSQNAYKEALAFLEGASVVVHEVVIVDKI
                     PKLLLERYGISHE"
     gene            complement(9517..10167)
                     /locus_tag="IWJ07_001965"
     CDS             complement(9517..10167)
                     /locus_tag="IWJ07_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333539.1"
                     /translation="MKKRFIYVCVGGLFLFGVGSTFFLIHEKLQLTAKEEKLTVEINK
                     FQEEKKGQEDLVTSLKEQLQQVESEVTTLKKANEELKNNDSTEKGNIQTFQKTVTEAF
                     EVLFNYEPDSFQERKNNCAPYLSDKLMKQYFKEGVSYGDSNGVSSELEKCSVYLKSTQ
                     KATIQGLVVVSYRNKIGDSSWNNAMNIFDVTFDPASKKITFMQNLGSSFNQQLLEE"
     gene            complement(10178..11350)
                     /locus_tag="IWJ07_001966"
     CDS             complement(10178..11350)
                     /locus_tag="IWJ07_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP9333540.1"
                     /translation="MKKKLLLWCGFGCLPTLLLCMFGFLFMILLISSGSEEEEIDCSP
                     TEIVASTISLTNQGMETNGLQIAKEVQKHIPNTTLEGLAGMLGNFQQESSMSPTAIER
                     PNDPLSGHGIAQWTAGRTTALKNFAKERNKEWSDLGVQVEFLIHELNTTEKASQVALQ
                     AKSVEEATEQWQVKFERAGIPMMGNRITFANQWYAKLGKNDSDPVASITLENGANAGS
                     KDLTDYCLETTGTSSSSDSNVLNVAKSLIGYFHYSQPQRAQFGSVENPDKNGYADCSS
                     FVWLVLTKAGYKTPANVGWFTGSMTSDARGSKQYLQEIPESEAKAGDIIIVNLGSGVG
                     NDGHTAVLAEDWHGLSTKVIEQGGMNTNGVGEGQVNLSFGYLLNGGDICFARPIKK"
     gene            complement(11369..12241)
                     /locus_tag="IWJ07_001967"
     CDS             complement(11369..12241)
                     /locus_tag="IWJ07_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9333541.1"
                     /translation="MKKMILGSLFSATVLLGMVGTSAYADTTSPGDPSVPPTEIIPPT
                     EETPEVPEEPVLPPTDGGEETPEIPEIPETPETPEIPPTEENPDTGGGTETPEVPENP
                     DTGGGEGTPEVPPVEENPGNGGNTETPEKPSNPGGNEGNTTPPTTPPTNNGGTNTTPP
                     VQDVVVKPDGSIGSQQTGGQNVQIETNNASELTHIPTPSTPIETASGEKIVSVVDGVA
                     YKESGNGLTPISDKVEQLPSGNIAVKGSDGKMKVLPKTGEEVSLVATALGGLLASGSG
                     FAFWRKRKATQKMN"
     gene            complement(12263..13183)
                     /locus_tag="IWJ07_001968"
     CDS             complement(12263..13183)
                     /locus_tag="IWJ07_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333542.1"
                     /translation="MMMIRFTEVDNMSLLTDKKIALDKAFIQLKRENEELLARAEIGW
                     CTLEIYTENHEIPVFEGSYDFPVEEFHLISLIEQSLTENGQLSEEAQTFIQTLNSELP
                     KFYQVKKDQKNERRPTKIKKEKKTPLSVETAFTQSNGSLQKKKMSFQWLLFGFIVVVS
                     CGLFIMQLSTQREIEQLTKKEHQLQERIAFAEELATKQPQIDTFSRYFITNYYSGEMA
                     EERYRKKLQSFLTEKVAKQISPQKEKVKSILSWEMKKEGKHWQIAYVITLETEKKTQE
                     TKKVSFQLEEQEKNYLVSTLPELDDFEINQ"
     gene            complement(13183..13452)
                     /locus_tag="IWJ07_001969"
     CDS             complement(13183..13452)
                     /locus_tag="IWJ07_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333543.1"
                     /translation="MIIVTGIVLLGIGVLFGYHFAYFPKIGEWQQRQAIQKYFQVKGN
                     EYTYYAEDEDSYIVSLNNQEYRVKFSLNNPIQVVYAVEVVEEVFD"
     gene            complement(13449..13829)
                     /locus_tag="IWJ07_001970"
     CDS             complement(13449..13829)
                     /locus_tag="IWJ07_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HAP9333544.1"
                     /translation="MITNYKKFWQNSFNYKGKATRKEYWLVALMNALIGFGLGLAIGL
                     AGALNASETIIGCLIGVLIVYCFVVLVPSLSLSVRRLRDAGFHWAFIFFYFVPTIGSL
                     VLLVLYCLPTKESIEDKQVQEETR"
BASE COUNT         3863 a         3031 c         2231 g         4867 t
ORIGIN      
        1 ttgtcttggt tcttttcttg tttcaattcg atatacattc ctagagaatt ttttgtcgta
       61 tctttccaag aaagattcaa cttcttggca ttttttacaa tcatttcttg ctagatacac
      121 aaaaaatgat tgattcgttt tttgaagttc tagtacttct tctaaattac tattgattag
      181 ttttccattt gattcttcgt tagtttttat cagtttaaaa caaccaaata agaggaaact
      241 aacaataagt atcctaacaa taactttctt atttcgcata ttgtttctcc aaatttggtt
      301 tccgaatata gacgactcgc ggtggttcct ggacgtcttc agctgttcga tacaccacaa
      361 attcgcctgt tctcaatgct cgaatgcgat tcggattgaa tttaaagcgg tctacgtctc
      421 gtttcgtgcc tctttctcct gtccaatcaa cctttcccat atcagaataa cctgcttctt
      481 gtgtcgtcat ttccgttaat tcaatgtctt cttctgtccc cattaaatct gcccaataat
      541 caatttctgt cgggtcattt gtcttcccaa taaagaacgt attcacattc ccaatcaact
      601 gttttcgaag gacaggatca attctatcaa tgtccgcaag cgtttgaggg gcatacgccg
      661 catgcacccc agcgcctcgt cccttattga ctaagtctat aaggttatca tttagataag
      721 aagctggttc gtcaaatatt actaggactt tcgagcgatt ggctgtttct cctgcttgtt
      781 tatacacttc tgacgcaaag aaattaatat ctcccacaag catttgagcc aatgtccgaa
      841 taaactcact ataaatcaat ccgtttaacg agatataaac aatctttcct tctttaacaa
      901 cttcttgaat atctaattct ctttttcctc cagaagtatc aaagagttca ccaagctcac
      961 tatccaataa catattgata ttggtctgta aattctcaga agtcgcatac aaataaaacg
     1021 ggttcttctt atgttcgtaa cgaacaaaca accgtttaaa caacgtttgc tcgatttctg
     1081 tcatgagaga aatgttttct tttaataata gataatacgt ttctaagtca acggttttac
     1141 gatcatgttc aacgattcgc tcttcactta actgttccac atcggcaaaa tcacgttctg
     1201 ttttgtccat cgcttcattc tttttcgttt ttttcttcgt ggttttcttc ttttctacga
     1261 ccactttttc tttaaataaa tctggatttt tcaactgaat acgatccgca aacaaatgta
     1321 agacgtttcg tggcaataaa taatgttgta accaaactag atttctttca attttatggc
     1381 tcttttggaa ctcgtctaaa agctgaatcg ttacctgtaa taacgctttg gctgctgtcg
     1441 aataatacac gctttctgtt tctgctagat tggttaattt atctcgaatg gcaatatcat
     1501 tgccattttt tacaggatta taacgcaaat cgccaccgtc cacaaagact tcaatcgggc
     1561 gattgtatgc ctctgccact tcttttaacg cttgtaaagt ctctcctgct cctttcccat
     1621 cgactaagat aacggggata cgaaacttac acgcatgttc tgctagtaat tggatcaccg
     1681 tcgtttttcc tgaacctgta gtcgctggaa taatcacatg ttggttcaat tcactaaagt
     1741 tcatcacata agggagccca tactcatctg ttcctaatag aatcactttg gctaatgaag
     1801 ccagttgttt attccgcaaa gcaacgtctt ttatgacaat tgctacctgg tacgcttgct
     1861 gatactctgc tgtatatttt tgggactcct gaaatttatt cttccgacgt tccttgtatt
     1921 ctttactatt tagcaaagct gtttgccgtt ctttctttga aacaactttt cttttttcaa
     1981 taaaagacgc atacgcaatc aaacaacttc ctgcacctgt cccacctaaa aggagggcaa
     2041 aaatcgtttg tccattaaac tgcaacgcat gagaaaacaa gtttaatgga aataataagt
     2101 aacacgacaa ataccctcta ggaagaacaa aaataaaaca aaatagattt aatagcgtaa
     2161 aacctaacgc aaaaataaaa aaaagagagc ttcttcgttt taagaagctc tcatactcaa
     2221 agtcataaat atgtgcacgt gtctcttttt tatctatcat aaagacaatc gctaaaggaa
     2281 tactgcctac aataaaaaat ggatatagac aaacacctaa taaagtgacg ataaaataaa
     2341 gttgttgaaa ccgacttttt ggcgtttctt tttcttgttt tcttttgcga tcgacagaaa
     2401 acatttgctc aaaatgtgtc ggtcgttttg gtgcattcat catactatcg atttcctcct
     2461 atcgttttcg ttgtttcatt tctcttcgat aaatcggttg tttttctcgt tttcgtttca
     2521 tttttgcttg agttgctcga aactcactcc gtgtcttcgg tgcttgtttt ggtgattttg
     2581 tttgtttcat ggttgagtat gctttagata cggttgaagg cgctttggtt tgtgctgtta
     2641 ctcgattgac tgctgacata tgttcccgaa ccccctctgg acgtcgaaca gatttcggtg
     2701 tggtagaacc tgttgctcgt ggtacattcc cttttcttag tggtttttgt accgcatgtt
     2761 tttgtcctgc aactgtccgg gttttcatcg aacttgtttt tgttacggga gtaagtgcct
     2821 ccttccgacg acgacttgtt tgtccctgac gacgttttcg atcgttcaat tctgttctag
     2881 gcgtatgctt ctggccataa gacttgtgtt tttgctttgg attcgctaat gacaccacca
     2941 ttgcagaacc ctgattgcga tacggactac gagacgcacc ttttctactt ctttctctta
     3001 gactaggatt gccttgcaat tctcgttgta ataaatcggc tgtttttctt gcctgtttct
     3061 ccattgctgt tcgttgcata cgttcttgac tttgctcttc tgcttgtttc gcttgatcat
     3121 gcaagtctct tgctttttgt gccatacgtt ccatacgatt tggtgcttta tgacgaggtt
     3181 gttgtgcgga ttcacgccga aaagcacttc gtgtggcttg tttcgccgtt tgtggcatac
     3241 gaacgtctga gccaccccca cgttttcgtt gacgttcttg tctcttccgt gcgttttctt
     3301 tttctttttg tttctgtgct tttctcgctt ctttgcgttc tttcaaccgt tgtttggctt
     3361 cccttctcaa ttttgcttct gccccaaatg gatttcccgc aaaagctaac gctcctcgaa
     3421 cacccattct tccagtaaat agactgccta aaaagcctcg gaacaaataa attcccaacg
     3481 gcgcaaacaa ataaacaatg atcaagagaa ttttttccca tggatcgtct tgtgtactac
     3541 ttaattggaa cccgtaacta agaaaagaag aaaaaaggat acatgcaaaa cctgtcattc
     3601 cttttaaaaa cgtcaaaata ccaaaaactt tggcatagtt accaaacaca ttcattgtga
     3661 caagaaacaa tcctaaaaaa agtaagaacg gcaacatcgg tactaacaat agttgcatag
     3721 ttccaataat caatcgaaac atacttaaga caaaataaac aacggtttga atcagattca
     3781 cgacaaaata aaacaaggca aatgctgtat ttttccaact attgtaatat tgtatgttct
     3841 tgtttttcag tgtatctgct tcatactctg tgactttgtc atgaattttc tcccctattt
     3901 ttgtacctaa atcattgtcc aataaaagat tgattcgatc atattctttt ccagcatatt
     3961 tgactttctt ctcacgaatt gtgtctacat tggttgtgcc ataatttaac aacagatatg
     4021 gttcataaac attactataa aagacccgag ccgccatcaa tttgcctgcc gtttccactt
     4081 ttttatccgc tgttacctct tctgtttctt ttgtttcata atttgtttct tcttcaggtg
     4141 ttaacactgg attgaccttc acgaacgccg tttccactgt agaatctaaa gaaaaaagaa
     4201 catcaaaaag attcttattt gtcccagaat cttttaatac gcttaaccca gtaaaaataa
     4261 ggatcgtcat aagaaagaca cggagtgctt gtttcattcg agcctctcca gcatatttga
     4321 ttgccatcat aattgccgaa aaactgattc caatagttcc tgcaatactt aacatattgt
     4381 ccgcaatttt ttgtgttaag ttttgaatgg gttctcgaat ggcggaaata atatctaatt
     4441 caaagagaaa ttcaaccatt gaagcattga attctcctaa catttttacg ccactccaaa
     4501 caaaattttt catcactcct gcaacattgt acgatagctt ttttttaagg ttcattgact
     4561 catagtcatt ggtcatcaat tcaaacgaat tctttttgta atttttatac aattgatcca
     4621 catcagtcgt tacttctttt acttctactt tatcgttggc attctctcct tcagaacgat
     4681 cggtttcagt gacacccgca ccatcattca tttgatcggc agaaacagta gccccttgta
     4741 gacagaaaaa aagaccccct aataaaagga gcgtccacca tttcagagat ttttttcgca
     4801 aaaaaaccac ctcttttttg ttttatcgtt gtttttcatt ttctaacatt gcttgaattt
     4861 tttcatcttg attttcagta gaagaaacag ccaataacca actatcaaac aacacatcaa
     4921 tggcaatcgg ttgatttctt cccaaatgat cctggaataa acaggttcct gtttgtaatt
     4981 gttccatcat ttctaaattg gctgaattca cttccatatc aaagaaggac aatagtttct
     5041 ctcgtgcttc tttttgtttc ggacgaaacg caaatttgta agaaattaac tcttttttat
     5101 cttcttgatc gtaatcttta aacgcttgca caacaaggta aatatccgta ttattcgcac
     5161 gtccttttcg caagctatca tcaatcaaat agcttccttg tgcggtatct tcataccctt
     5221 ttgcctcgtc ataaataatc gcagcgtctt cttctttgtt tgtagaaaag atatacgtaa
     5281 acttcataat gacttccata attgccattc ctgcaagttg ttcgctattt aatcgacccg
     5341 aatcaatctc tttttgtgtc ggaatgcgta ttccttgtgt ccctaacacg ttgatttgtg
     5401 aatcaaaacg tatcggcgta ctgtgctcat tcccaatgag aatctttcct aaaccaacgc
     5461 tatgccctaa aatcaattta gctagttctt tgtcactttt tcgaatttct tcgataactt
     5521 gacttaacga atgcttacct ggcatggtgt aaactacatc gatggcgtct tggatcaccg
     5581 ttttcttgtg gctcgctgtt tctcgattgg tatctacttc tcctaacatt cctaacacat
     5641 ttttagcggt ttgaattgcc tcttcttttg gtaaaaacaa tagaggatca agcatccctc
     5701 gacagctttc atctgaagat aacgaaataa aattaattcg ttcatacata gccacaaact
     5761 ctggtatgtg tccgaatttt tcaagtgcct ttaaaaagaa cttttgggtt tcatttttcg
     5821 gatcaatata aagaattttt tgacctaaaa acatcagcca taaaaaaata tattttacaa
     5881 ggaccgattt tcctttacct ggtggaccaa taatcaacgt atttccattg gtatgttccg
     5941 cgccttcaat acttttcttt gttaaattgg gataatacca tactagttta ctagatagcg
     6001 ctaacgcttc tttcgcagat ttaaactttt ttgcaataac aatccgtcct aaaggaaatc
     6061 ctttcttatt tcctacttct ttctttaagt ccaagccata atctgccaca aaacctgtcg
     6121 tagcgacctg ctcgtaacta cgaaaacgtt gcgtacttcc aattaagcat tgattaaaca
     6181 acgttagctg atccgctacc gatcgataaa atttgaaatc tgtagactca atcgcaaatt
     6241 caatatcttt gactcgttct tccaattctt cttttgaaga agcagataca accagcgtga
     6301 cggtagaata acacaatcgt ttttctttgc gcttcagctt atcttttaca tccattaacc
     6361 gttctgtacc aaaatttaat acatcgtctg aatctaaaat gccgtcaacg gtctcatatt
     6421 ctttttcttg ttcataaatc cgtccagcca ttttattaat ttttcgttca tcagattttg
     6481 aatgctcaaa tcgaagtcga atatgcgtat caattggaaa agataagcta tcctgaagat
     6541 tttgaataaa gctactccca tacatggcta aaggagcgtc cgtttgactt aaaaacgtcg
     6601 aataatgcgt ttgattcaac tgttcaatcg caatgtatcc tgcttcattc gaaatgacgc
     6661 cctcgcaaat ttccattggc gtaagctgtc tttcaggcaa tttttcttct gatcgatgga
     6721 aaaaataata ataaatttgt ccaatctctt gggcagataa gcgctctatc ggcttataat
     6781 tggacaattc cgtatagagc tccttttcta atgtttcata cgttggtaac acactactta
     6841 taggaactcg ttgtcccgta atacgataga taaacttttt aaaaacatgc ttaagcaatt
     6901 gaaaatattc gatcggatca gacactttca tttctgtagt gaaacgcaca aacagatacg
     6961 ttcgatactc gtttaattgc acttcgtctt ttaaaatctt tcctgcctgt tcaaagtaca
     7021 ctttcccaag atctgcgcat tctcccttta ccaaatctcg aatggtttct tcaatgtgcg
     7081 tatctaggtt aaattgacgt ggaatattga taaaatgata gtcatattca tcatgtccaa
     7141 gtatgccttc gccatcttga agatagttct tgaaaaaatc aatgtcattt aaaggaaata
     7201 cttgggaaga aagttgatag cctacccaca catcatcatt tttggtcaaa acaaaatttc
     7261 ggataatggc gtctatcgga aattctaatc gaggaagctt cgtttttttc tttttgaact
     7321 gccaaatccc gataataaag acgaccaaaa gaaacaaaaa cgccatttac tcgactcact
     7381 ccttccattt gtgtagggaa tacgtcactt tttcatggaa aaacggcaca tacatttcat
     7441 gttcaaagcg ttgtttccgt ttctgataat aggacgttcg cccaattaaa tactcgaaaa
     7501 agtgtttatt gtcccatttc attgaaaaca atgtccaaac gagaacacca atcccaaaaa
     7561 tcataatcaa ccaggacttt cgaaacagtg tctgcaagaa actaatttga ttcacatacg
     7621 caatcaaaaa taggatcaaa agaatgaaga gaaatacccc acctatgact aattgttcca
     7681 tgcgaatgcc attagaaatc cagggaattt tttgtccttt aatcccgtaa attttgtgag
     7741 ggtgcttaaa ctcctttgta tagttgtatt ccatgttcac tctacctcct tttacaaaaa
     7801 aagaagcgac cagaagttct aatcttctga tcgcttttgc agtttgtctt tttcttatga
     7861 taattgtatc cagggcacaa tcatttgcgt tacccccacg ttctcttcac tatccttaat
     7921 aaagataatg ttctttagcc attcaccaat atcaacggca ttcgataaca ataagacgag
     7981 cattatcaaa ataaagacgc caataatggc aaacgctgcc cgcgcaaagc cttgggtcgc
     8041 ataagcacca accattagaa caatcgcaac gattgccaat agccaaatga cttgtccaga
     8101 tacatcatca aataacgatt taagatcaaa aagtttcatt tccattctcc tctaagttca
     8161 ttttattcat ttaacgtagt aacaaaataa ctatcgccac ttgtttttac ttccattgaa
     8221 aagtttgatt ggaactcgtt tcctttctca aaatagaaac gatacttccc ttgcaaaaag
     8281 tagtttcctt ctttattttt tactgcatga tccagttgta cattgatcaa ttcgccgcct
     8341 tctaaccctt tttcgttaga aatcaatgcc aatttttcat catttttgac atataactca
     8401 aaaaaccgtt tcacaaatgc ttccagcttg atttcttcgc tttcagtgac cgcttgccca
     8461 gaagaataaa atcgattttc ctgatagttc acagaattgg tattcttact ctcagaaaga
     8521 ttcaaaaatt gaacgccatc aagtactttt aaaacattgt tttgataact gactggaaaa
     8581 gaaagttgac tgcttttcca atgttctcct tctgtaaaag taacctcata aaataggcga
     8641 tacattgctt gtgtcaaatc gatttcttct agtttaataa agcgaacatc tttcgctact
     8701 cgattggtag ccgtcgttac aaagcctaga tcagacgctt ccaatccttg tgctaaataa
     8761 tgattgacgg aagcctccca ttcgtttcgt ttttctggcg aataagtaaa taacctttca
     8821 ataaaatgtt gtccctcgta cttcaattgt tccgtagaag acaaggtttg gttgacttca
     8881 gttaacaatg tttctggatt gacttgtttt ttcactgcta aacgcgttaa tgtatccact
     8941 cgtccaaagt ttaggaggag cacgacaccc attaacagaa acaaacaaaa aaaagcgcct
     9001 gaaaatacac tcatggcact tttttgcgaa aattggatca ttcgatctga cggttctgtt
     9061 ttcttttttt ggaacaaaaa acgtttcttt tgtttctttt ccttctgttg tttcggtggc
     9121 ttagtttgga acgcttgtgg attaaatttt gcattactca tgagaaatgc cataccgttc
     9181 taacaataat ttcggtattt tgtccacaat cactacttcg tggacaacca cacttgcccc
     9241 ctccaaaaag gcaagtgctt ctttgtaggc attttgactg tatttttttg ataaagcatg
     9301 acaaatttct tgataaaata gttgactgcc taccgaatac cgattattgt gtatccgcca
     9361 caaaacgaac acactgattt gaatcgacgg gcgtaaaaaa gaaaaattct ttccataaga
     9421 aaaagaacgt ccttcttggg tttcttttgc ttgttctgtt ggtacttctt gtacctctct
     9481 tattgattct tgtctctcca taaccttctc cttttcttat tcttctaaaa gctgttgatt
     9541 aaagctactt cccaaatttt gcataaacgt gattttcttt gacgctggat caaaggttac
     9601 gtcaaaaata ttcatggcat tattccagga actatcacca attttattgc gatacgacac
     9661 cacaacgagc ccttggatag tcgctttttg tgtgcttttt aaataaacag aacatttctc
     9721 taattcagaa gacaccccgt ttgaatcacc ataggaaacg ccttctttga aatattgctt
     9781 catcagtttg tcagaaagat atggtgcaca attgtttttt cgttcttgaa aagaatcagg
     9841 ctcataatta aacagaacct cgaacgcttc tgtcactgtt ttttgaaagg tttgaatgtt
     9901 tcctttttct gttgaatcat tgtttttcaa ttcttcattt gcttttttta acgtcgtgac
     9961 ctcgctttct acttgttgaa gttgttcttt gagtgacgtt acaaggtctt cctgtccttt
    10021 cttctcttct tggaacttgt ttatctccac cgtcagtttc tcttcctttg ctgttagctg
    10081 tagcttttca tgaataagaa aaaatgtact gcctacacca aataagaaca atccgcctac
    10141 acaaacataa ataaatcgtt tcttcatttg acacctctta tttctttatc ggtcgagcaa
    10201 aacaaatatc accaccatta agcaaatacc caaaggataa attgacttgc ccttcaccta
    10261 caccgttagt attcattccc ccttgttcga ttactttcgt agaaagtcca tgccaatctt
    10321 ccgctaaaac agctgtgtgt ccatcatttc ccacaccaga acctaagttc acaatgataa
    10381 tatcgccagc ttttgcctcg ctctcaggta tttcttgtaa gtattgctta cttcctcttg
    10441 cgtcgctcgt cattgagccg gtaaaccacc caacgttcgc aggcgtttta taccctgctt
    10501 tcgttaaaac aagccacaca aagctcgaac aatcggcata gccattttta tctggatttt
    10561 cgacactacc aaattgtgcc cgttgcggtt gtgaataatg aaaataacca atcagacttt
    10621 ttgctacgtt taatacatta ctatcggaag aactgcttgt tcctgttgtt tctagacaat
    10681 aatcggtcaa gtctttactt cctgcgtttg ccccattttc taatgtaatt gaagcgacgg
    10741 gatcactgtc attctttcct agttttgcgt accattgatt cgcaaaagta atacgattcc
    10801 ccatcattgg tatccctgca cgctcaaatt tcacttgcca ctgttctgtt gcttcctcta
    10861 cgctttttgc ttgtaatgca acttgtgaag ctttttctgt tgtgtttagt tcatgaatca
    10921 aaaactcaac ttgtacacct aaatctgacc actctttgtt tcgctctttg gcaaaatttt
    10981 tcaaagcagt cgttcgccct gccgtccatt gtgcaatccc atgcccagat aatggatcgt
    11041 ttggtcgttc aatcgctgtg ggactcatac tcgactcttg ttgaaaattg cctaacatac
    11101 ccgctagacc ctctaatgtc gtatttggaa tatgtttttg tacttccttt gcaatttgaa
    11161 gcccgtttgt ctccatgcct tggttcgtta atgagatagt acttgcgaca atttcggttg
    11221 gactacagtc gatttcttcc tcttcagaac cactactaat caaaagtatc ataaacagaa
    11281 aaccaaacat acatagtaat agtgtgggta gacaaccgaa cccacaccat aaaagaagtt
    11341 ttttcttcat gtttgatcct tcattcgctt agttcatttt ttgtgttgct tttcttttgc
    11401 gccaaaaagc aaaaccgctt cctgaagcca ataatccacc aagtgctgtc gcaacgagag
    11461 acacttcttc gccagtctta ggcaatactt tcatttttcc atcagaccct ttcaccgcaa
    11521 tatttccgct tggaagttgt tcgaccttgt ctgaaatggg cgtcaagcca tttcctgatt
    11581 ctttataagc gacaccatca acgacggaga caattttttc tcctgaagct gtttcaattg
    11641 gtgtacttgg tgttggaata tgggttaatt cagacgcatt attggtttca atttgaacat
    11701 tttgtccacc tgtttgttgg ctgccaatac ttccatctgg ttttactacg acgtcctgta
    11761 ctggaggcgt agtgttcgtc cccccattat tggtaggtgg tgtcgttgga ggagtcgtat
    11821 ttccttcatt gcctcctgga ttcgacggct tttctggtgt ctctgtatta ccaccatttc
    11881 ctgggttttc ctcaacgggt ggaacttcag gtgttccttc ccctccgcca gtatctggat
    11941 tttctggtac ttcaggcgtt tctgtgcctc cacctgtatc tggattttct tccgttggtg
    12001 gaatctctgg ggtttcagga gtttctggta tttcgggaat ttctggggtt tcttctcctc
    12061 catctgttgg tggtaaaaca ggttcttcag gaacttcagg tgtttcttcc gttggtggaa
    12121 taatttctgt cggtggtaca cttggatcac ctggtgacgt agtatctgca tacgcactgg
    12181 ttcctaccat acctagcaat acagttgcac taaataaact tcctaaaatc atttttttca
    12241 tgtgtttttc ctcttctctt atttattgat ttatttcaaa atcatctagt tcgggaagcg
    12301 tggaaactag ataatttttt tcctgttctt ccaattgaaa agacactttt tttgtttctt
    12361 gtgtcttttt ctcggtttcc aatgtaatga catacgcaat ttgccaatgc ttgccttctt
    12421 tcttcatttc ccaggataaa atacttttga ctttttcctt ttgaggactg atttgtttcg
    12481 caactttttc cgttagaaac gattgaagct ttttcctata ccgttcttct gccatttcac
    12541 ccgaataata attcgtaata aaataacgac taaatgtatc aatttgtggt tgttttgtcg
    12601 caagttcttc cgcaaatgca atgcgttctt gtaactggtg ctcctttttc gttagttgtt
    12661 caatctctct ttgcgttgaa agttgcataa taaaaagacc acaagataca acgacgataa
    12721 atccaaacaa aagccattga aaagacattt ttttcttctg aagagagccg ttgctttgtg
    12781 taaacgctgt ttctacagaa agtggtgttt tcttttcctt tttgattttt gttggtcgac
    12841 gttcgttctt ttggtctttc tttacttgat aaaacttcgg taattcggaa tttaaggttt
    12901 gaataaaggt ttgtgcttct tcacttaatt gcccattttc tgttaaagat tgttcaatca
    12961 atgaaatcaa atgaaactct tctactggaa aatcatacga gccttcaaat actggaattt
    13021 catggttttc tgtgtagatt tccaaggtac accagccaat ttccgctcgt gccaatagct
    13081 cttcattttc tcttttcaat tgaataaatg ccttatctaa tgcaattttt ttatctgtta
    13141 gtaaagacat gttatcaact tctgtaaatc gaatcatcat cattagtcaa acacttcttc
    13201 caccacttct accgcataca ccacttgaat agggttattt aaagaaaatt tcacccgata
    13261 ttcttgattg ttcaaactga cgatatagct atcctcatct tcagcataat aggtatattc
    13321 gttgcctttg acttgaaaat atttttggat cgcttgtctt tgttgccatt cgccaatctt
    13381 tggaaaatag gcgaaatgat agccaaataa aacgcctatc cctaataaaa caattcctgt
    13441 tactataatc atctggtttc ctcctgtacc tgtttatctt ctattgactc ttttgtcggt
    13501 aagcaataaa gaaccagtaa cacaagagag ccaatcgttg ggacaaaata gaaaaaaata
    13561 aatgcccaat gaaaacctgc gtctcgtaaa cgtcgaacgc tgagagaaag agacgggaca
    13621 agaactacga aacaatacac aatcaatacg cctatcaaac aaccaattat tgtttcagaa
    13681 gcattcaacg ctcctgctag tccaatcgct aagcctaggc caaacccaat gagggcattc
    13741 attaacgcaa cgagccaata ctcttttctt gtggctttcc ctttgtaatt aaagctattc
    13801 tgccaaaatt ttttataatt tgtgatcata tctttgtcct ttctaatgtt tatttttgat
    13861 tctagtttct tctgttttac taacaatgaa acttgccata aaaaaggtgg ccaatacacc
    13921 accagctaga aatccactta caaataacaa aattttttcc tcctttctaa aaaatttcgc
    13981 cataaaaagg ca
//
LOCUS       DACDPS010000062        13948 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.062, whole genome shotgun sequence.
ACCESSION   DACDPS010000062 DACDPS010000000
VERSION     DACDPS010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.062"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..758
                     /locus_tag="IWJ07_001971"
     CDS             <1..758
                     /locus_tag="IWJ07_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9333545.1"
                     /translation="TVIHYHAFIRKALQEAVITELISTNVADKVRKPKKKQFVSQVYN
                     QQEILKLLDILSEEKLYLVVLLTAFYGLRRSEVLGLKWSAIDFLNKKIMINHVIIENP
                     ENMSQLIKKDQTKNSSSYRTLPLVETIERALVNQAKWQQDNRILLGDDYILEDNEYVF
                     TMEDGRLMKPQYVTHRLSKLIKKNGLKKIRFHDLRHSNASIMLDSGQNMKSIQEWLGH
                     ASYSTTANLYTHLTAGVKDRAAEALENAFGFKE"
     gene            1332..1901
                     /locus_tag="IWJ07_001972"
     CDS             1332..1901
                     /locus_tag="IWJ07_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAP9333546.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1888..2748
                     /locus_tag="IWJ07_001973"
     CDS             1888..2748
                     /locus_tag="IWJ07_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9333547.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2938..3642
                     /locus_tag="IWJ07_001974"
     CDS             2938..3642
                     /locus_tag="IWJ07_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9333548.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            3647..5482
                     /gene="walK"
                     /locus_tag="IWJ07_001975"
     CDS             3647..5482
                     /gene="walK"
                     /locus_tag="IWJ07_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAP9333549.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            5479..6795
                     /locus_tag="IWJ07_001976"
     CDS             5479..6795
                     /locus_tag="IWJ07_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333550.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            6796..7665
                     /locus_tag="IWJ07_001977"
     CDS             6796..7665
                     /locus_tag="IWJ07_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333551.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            7720..8529
                     /locus_tag="IWJ07_001978"
     CDS             7720..8529
                     /locus_tag="IWJ07_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP9333552.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(8632..9261)
                     /locus_tag="IWJ07_001979"
     CDS             complement(8632..9261)
                     /locus_tag="IWJ07_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HAP9333553.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            9431..10723
                     /locus_tag="IWJ07_001980"
     CDS             9431..10723
                     /locus_tag="IWJ07_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9333554.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(10799..11461)
                     /locus_tag="IWJ07_001981"
     CDS             complement(10799..11461)
                     /locus_tag="IWJ07_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP9333555.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            11686..11970
                     /locus_tag="IWJ07_001982"
     CDS             11686..11970
                     /locus_tag="IWJ07_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAP9333556.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            12021..13646
                     /gene="groL"
                     /locus_tag="IWJ07_001983"
     CDS             12021..13646
                     /gene="groL"
                     /locus_tag="IWJ07_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAP9333557.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4683 a         2360 c         2968 g         3937 t
ORIGIN      
        1 atacggttat tcattatcac gcatttatac gcaaagctct tcaagaggct gttattacag
       61 aattgataag tacaaatgta gcagataaag taagaaagcc aaaaaagaaa caatttgttt
      121 ctcaagttta taatcaacaa gagatcttga aattacttga cattttgtca gaagaaaaac
      181 tctatttggt agttctttta acagcatttt atggattgcg aagaagtgaa gttttaggat
      241 taaaatggtc tgctattgat tttttaaaca aaaaaatcat gataaaccat gtaatcatag
      301 aaaatccaga gaatatgtca cagttaataa agaaagatca aacaaagaac tcttccagct
      361 atcgtacatt acctttggtt gaaacgattg aacgagcatt agtaaatcaa gctaaatggc
      421 aacaagataa tcgtattctt ttaggagatg attatatttt agaagataat gagtacgtat
      481 ttactatgga agatggacgg ttgatgaagc ctcaatatgt aacgcatcgt ttgagtaagt
      541 tgattaagaa aaatggattg aaaaaaattc gctttcatga cttgcgtcat tctaatgctt
      601 caataatgtt agatagtggc cagaatatga aatccattca agaatggctg ggacatgcat
      661 cgtattcgac aactgctaat ttatacacgc atttaaccgc aggagtaaaa gatcgtgcag
      721 cagaagcatt agaaaacgca tttgggttta aagaataaaa aaacaaaaaa caatatcttt
      781 ggggtatcgc ttcttgtttt tagaattttt ttgttggcaa tttgttggca gtatgttggc
      841 aatgaaaaaa ttcacaaact aaaaacctat ttataaagct ttataaaact tttttcacaa
      901 atagagcatt tggttaagag tcagctgctc taccaactga gctaacggcc cgtacaaaaa
      961 gtacttaata atagtagcac taagaatttt aaaatgcaag actacattta aagaaaatca
     1021 gaaaaatctt tacttcctta taggaaatca ttcatagatt attcacattt gctcgctatt
     1081 atataagcat accaacaaac aaagttcttg caagagaaca aaccctaaca cttttttcat
     1141 tttttactcc tttcccgccg tctccccggc gggtctcctg ttcttaacga taaaagacaa
     1201 gaaggcttag ccttcttgtt ttttgttttc atggaatccc gctaatcaaa tcacctagtt
     1261 aaagcaacta gattgaatgt ttatcataag tctgatatca tgtttatgaa atgaaaaaag
     1321 gagcggttat gatggaaaaa atgcgtcagc aattaatgga gtgggggaag gaactagaaa
     1381 cattccgtct gcctcactgg gaagaactcc ctgacttaga actttatatg gaccaagtca
     1441 gaacgttagt tgatcgttat ctttcacctg tgatacaagg agaaaagcat ccattgctta
     1501 cctcttctat ggtaaacaat tacgttaaac taggtttgat accagcacca gttaaaaaaa
     1561 gatataacaa agaacacgta gcatttttac ttgcaatcac tacgttgaaa caagtattga
     1621 ctattcctga aatcaaagaa gggatattgt tccaaggaaa aacagtagga attcgtgaag
     1681 cctataatct attttgcgat gaacaagaag cagctgtgtg gatggtcagc caattagcac
     1741 aaggtaaatc tcatccgcaa aaattcgacc aggcaacccc tgtagaatat attgctgtaa
     1801 gagcggcaac tttatcgttt gcaatgaaac ttcttgcgga aaaaacgatt gttctagaaa
     1861 ccgaatattt aaaagaggag aaaacaaatg aaaaacaata aaatcgcaat tttagtagat
     1921 tcaggtacgg atgtgccaga agaattgata aaaaaatacg agatgtatat gatcccattg
     1981 aaaattattt ataaagatca ggtatataca gataaaattg acattacctc tgaagagatc
     2041 tatcaacgtt tgccagagga aatccctggt acatctttgc cggatggtga gtcgatcaca
     2101 gaaatctttg agcaaatcaa aaaagacggt tatgaaaagg tacttgcggt aacaatttcc
     2161 agtggattga gcgggacata taatgtggtt cgtttaattg cacaacagca agaagaattg
     2221 gaagcatatg tactagatac gaaaaatatt ggtattggtg caggtttttc tgcgatccaa
     2281 gcagcaaaat ggctggaaga aggagtagaa tgggatcaac tgatcagtaa tctgaatgag
     2341 ttagtcgaaa gaacaaaagt tttctttaat gttgcgacat tggaatatct tcaaaaaggt
     2401 ggacggatcg gtttagttgc ttctattgta ggaaccgctt taaagcttaa cccaatcatc
     2461 tcatgtaatg aagaagggat ttattacaca gtaggaaaag ctaggggacg taaaaaaagt
     2521 ttggataaaa cggttgctta cgtcaaagaa aaaatcggta atgcaaaggt cttcaactta
     2581 gctgttgccc atggggatgc gaaagaagaa gcaattgaaa tggaagcgag acttaaaaga
     2641 gagttcccac aagcaaacca aatctacttt ggccagattt ctccggcatt agtagtccac
     2701 actggtccgg gattattggg tgtcggtgta caagttttag aaggataaat caaaaaaagg
     2761 aaacactgag gtgaaaatca gtgttttcct tttttattga agttagaaca aaaaataata
     2821 aagtattttg cactttcatt ttaattttct cttttgtttt ataggcaaag atgaaacaaa
     2881 agcagaaata cggtataatg agaaggtatg acaataatat ataagaggtg gctctttatg
     2941 aaaaaagttt tagttgtcga tgatgagaag ccgatttcgg atatcgtcaa attcaattta
     3001 gccaaagaag gctatgatgt ttatactgca tatgatgggg aagaagcctt ggaaaaagtt
     3061 gctgaggtag aacctgattt gattttactt gatttgatgc tacctaaaat ggacggctta
     3121 gaagttgctc gtgaggtacg caaaacttat gatatgccga tcattatggt aacagccaaa
     3181 gattctgaaa ttgataaagt cctaggctta gagttaggcg ccgatgatta cgtgaccaag
     3241 ccattttcca accgagaatt agttgcacgt gtcaaagcaa atcttcgtcg cggtgccacg
     3301 gctgcgaagg aaccagaaga ggcagcacct gcggaattga cgattggtga tttgacgatc
     3361 catccagaag cttacatggt gacgaaacga ggagaaacaa tcgagttaac acatcgtgaa
     3421 ttcgaactac tcttttactt ggcgaagcac cttggacaag tgatgacaag agaacattta
     3481 ttacaaacgg tttggggtta tgattatttt ggagatgtac gaacagtgga tgtaactgta
     3541 cgtcgtttgc gtgaaaagat cgaagataat ccaagccatc caaattattt agtgacacgt
     3601 cgtggtgtcg gttactattt gcgtaaccca gaacaggagt aattggatga aaagaaaagt
     3661 ccgctttttt cgatcagtca attttaagat cgctattacc tttattctga ttttattgat
     3721 ctcgatcgaa attattggtg cctactttat acgtggttta gaacgttcca cgatcaatac
     3781 atttattaaa gatatgaatc aaacggtgga atcattagca acaacaatca gcccggaatt
     3841 gaatcgaaaa gataatgcag atgatgaaga ggtaaatgcg aatatcaaaa gatttattga
     3901 aaacagtgcg actagcgata tcatcgaaat acgggtcgtt gacgaaaaag ggatcatccg
     3961 aggaacaacc gatgtcaatg aacaaagcgc tgtgggaaag aaaaatgatt atgttgatat
     4021 caatgatttc acgactaaac gttatgttgc tttagataac gacgataaac gggtgaatat
     4081 caatgttcaa ccgatcctgt ccccgaccgg cgatgccgtt atcggggctc tttacgtcaa
     4141 aagtaatatc gaacaaaaat attcggaaat caataacaca gcagtcattt tctttactgc
     4201 ctcactaatt gcggcattta tttcaatgat cgtatctgtt ttagttgctc gttcgattac
     4261 acagccgatc ggtgaaatga gagaacaagc cattcgaatc gctagaggag actacagtcg
     4321 gaaagtcaaa gtgcatggac aagatgaact tggacagctg gctgatacct tcaatcagtt
     4381 agctgaacgt attgaagaag ctcaagatac aatggaagct gaacgcaatc gtttggatag
     4441 cgtgctatcc catatgacag acggcgtcat tgcgacagat cgcaggggta aagtcattac
     4501 gatcaatgat atggctgttt ccttgctaga tatcaaaaac gaggaagcaa tcggacaatc
     4561 gattttgaca cttttagata tcgaagaaga atatacgttg cggaaattat tagaagatcc
     4621 aaacgaaatg gtacttgacc gttcaaccag tacattggaa agtgatcaga tgatcttgcg
     4681 agtagacttt tctatgatcc gtcgcgaatc aggttttatc agcggattag tagctgtctt
     4741 gcatgatgtg acagaacaag aaaaaacaga acgggaacgt cgtgaattcg tctctaatgt
     4801 ttcccatgaa cttcgtacac cgttgacaag tatgcgtagt tatatcgaag cattgagcga
     4861 aggtgcgtgg aaagacgagg aagtagcacc aaattttttg aaggtaacgc tagatgaaac
     4921 agaccgaatg attcgaatga tcaatgacct cttaaatcta tctcgcatgg atacaggtaa
     4981 tacccagctt caattagaat atgtcaattt caacgagtta gtgaattttg ttcttgaccg
     5041 gtttgatatg atggtgggaa atcaggaaaa aaattataag atccgtcgtg aatttacgca
     5101 aagagatctt tgggtcgaag tagatacaga taaaatcatc caagtagtcg ataatatcat
     5161 gaataacgcg atcaagtatt caccagatgg gggaacgatt acttgtcgct tattagaaac
     5221 gcataataac gtggttttaa gtatcaccga tcaaggattg gggattccta aaaaagatct
     5281 gaaccgagtg ttcgagcggt tctatcgagt agataaagca cgtgcaagag cacaaggtgg
     5341 gacaggactt ggattagcta tttctagaga agtgattaag gcgcatcgag gcgctatttg
     5401 ggcagagagc aaggaaggaa aagggtcgac tttttatatc tcgctgccat atgaacctta
     5461 tgaggaggaa tggtgggaat gagaatctct gaaaaagtcg ttcgtatagg actcattttg
     5521 atggttgcgt taagcatata cttctcttat gcaatctggc tgagcccagc aggaaaaagt
     5581 tcaatcaacg tagatgagac aaacactcaa atgatcgagt ctcaaagcaa tcgaaaaact
     5641 tccgaagtct ttcttccgct tcatgttaca tggcaccatt cgggaatgat ccaagaaacc
     5701 aacagtgaaa atctcgtgta ccgattgcaa ttgatcatcg ataaagcaag atttggaaag
     5761 ctttctgaag tagtttcagg agacaaatca cagtttgaac aggtgaaaaa tctgaactct
     5821 ggttttgagt tggcctataa tgcgcctttt ttgctaagag aatacaagga agcatttgat
     5881 ttagatttag atgtgtcgaa cttggcaaaa gaaagcgaaa atatgtattt tactagaata
     5941 caattcgatc aaggaaataa aaaaatccga tttttgaatt attcgaacca tctcgtatat
     6001 gaagcaaata tttcgttgaa ttggacagat atcaataaag aattaacagc aaaagacaca
     6061 aaatggacag aaatgacaac agaaccatcg acgatcgaca cgcaatatgc tacgaaacat
     6121 tcgatcaaac tgaaaaagta tagttatatc ctgtcacagc aaccctatac actttttaga
     6181 aatgcttttt tccagaaacc agatagcgtg aaaaataatg atgaaagcaa tgaattgatt
     6241 ttttacggtg ggaatgaaac aatgtctatc cattcggaaa cgcaagtagt tgattttcgt
     6301 ggagagctgc cagagaaaaa agaaaatgct agtgtatttt ctgaaagttt cccctatatt
     6361 agcaaattag gcagttcttt agggaacctc agatattttg accgcagtga caatacgatc
     6421 gattatcgga tattcgtgga aggcttcccg gtttttggga cagacagcag aggacagatt
     6481 ggtgtggaag ttggcggtga aacgaatggt tctgtgcaag tgaatatcca aaccagtttg
     6541 aatacgattc aagtgccgat accttcagac gaagaagtag aacttgcaag tacgaatgaa
     6601 atcatccagc agttaaaagc taagggagca gatagcaaca agattggttc agtgatcgtt
     6661 ggctatacat ggcaaaatgt caaagaaacg aatcgtgtcg ttgatctctt acctgaatgg
     6721 tatgtgaaat ataaagataa ctggtattca gctaacgacc ttctggaaaa attacctgga
     6781 ttggaggcga attaattgga tttcaaaaaa atcgaatgga tatttttcgt cgctttttta
     6841 gggttgaatg tgtttttatt cagcagttat catgacgcgg tataccaaga aaacaatgtc
     6901 attcgctcta atcagactga atcaatccaa caacgtctgg ctagcgacga tatcacttac
     6961 acggggaaga tttcgtctga gaacaagcaa ggctattatc ttagcgcgga gcagaccaat
     7021 cttagcactt cattagcgaa ttatcgcaag gctggtaaaa gcggcttgct ggcaagtggt
     7081 gtgtttttag acggtgatgt tttgacgaaa tctttatcca gttcagatgc tgaaaagaac
     7141 gtgatcgatc cagataaaat tagtagttcc ttggaagatt ttttatctaa caaagacaat
     7201 attttattcg gtaatgatta tgtgtacacg aaaaactttt ctcatttaga agaagaacca
     7261 ctagaaatcc aagcaagcca agagtatgaa ggcatcccat tcaatgatga tacagcaaaa
     7321 atcgttcttt ctttagaaaa aaacgataat gattatagca tcacgaaata catgcagacg
     7381 catttatctg atatcgagca gctgcgcgaa aagacggaac tgtatacgga agaggaagca
     7441 atcaatacat tgtacatcaa taacaaaata ccaagaggat caaaaatttt atggcgtcag
     7501 ctagcttatt cctgtattct gaaagtccgg gaaaaaaatg tgtatgttcc tgtttggcat
     7561 gtagcgatcg aaacaagcga taaagtggtc caaattgaga gtgtcaacgc tttcagcaat
     7621 acgatcgtga caaacaatac tgtaccaaaa gtggaagatc aataaaaaag cgagtataat
     7681 gaaaaaagtt tacaaagtaa gaagggaagt aggagtcaaa tggctgaaga aaaaccagct
     7741 tttaaaatca gtatcctagc aagtggaagt tcgggtaatt cactttatat cgaaagtgaa
     7801 aaaaaacggc ttttggtcga tgcaggactt agcggtaaaa aaatcacttc cttgatggct
     7861 caaattgatc ggacaccgga ccaattagat ggaatacttg tgacgcatga acatcgagat
     7921 catatacatg gcgtgggtgt cttggctcgt aagtatcatt tggatgtttt tgccaatcag
     7981 cagacttggg aagcgatgga accactgatt ggaaatgtac cagtggaaca aaaacatcta
     8041 tttgagatgg ggaaagtcag aacattcggc gatatcgata ttgagagttt cggcgtctcc
     8101 catgacgcag cggctccgca attttatcgt ttccataaag aaaatcggtc attcgtcatg
     8161 ttgacggaca ccggatattg cagtgattat atgcgaggtg tcattgaaaa tgccgatggt
     8221 tatttgatgg aaagtaatca tgatcttgag atgttacgca tgggacctta tccgtggaat
     8281 ttaaaacagc gtattttagg agatagagga catctttcca acgaagacgg tgcacttgtc
     8341 atgactgatg tgatcggtaa tcggaccaaa cgaatctatc ttggccatct tagcaaggaa
     8401 aacaatatga aagaacttgc tcatttaaca atggaaaatg ttttgaaaga aaaagatttt
     8461 ggcgttggac atgattttga aatctatgat acagatccag atgaagcaac tgagctattt
     8521 tcgatttaaa cagttcaatc aataaaaata taaaaagcag aagataaaat caatcagggt
     8581 tggttttatc ttctattttt atttttttag catattaaaa aaataaccgt attatcgact
     8641 gactacactg atgtgctgct tttcatgttt ctcatccagt atctcatcta ctaatgcaat
     8701 cgcgtaatct gccatgctga tcgtactgtc gccagcagcg tttctttgta aataatcatc
     8761 acttaacaaa taagtacctg tacgtttgcc atgtggatca aataaagaag ctggactgat
     8821 ataagtccaa tgtagatttt tagattgttt caattttaac aatccttcgt acatattgaa
     8881 agctgttgga tagaagggtg catttttagg tacttggtca atcatacgag tcgtcttttc
     8941 tgtatctaag aataacgaag aggcaccccc aacgacaatt aagcgtgtat cggttccttc
     9001 aagcatctca atcaaatggg acaaagaagt ttgatgcatt tcttcttttc ctttgggagc
     9061 gttgaaagca tcaacgacaa catcaaaaga tgcaagatca gtagttgtca actcaaataa
     9121 atccttttgt ataaacggaa catctgatgg caatttttct ggatgacgca cgattgccgt
     9181 cacgtctaaa tctctggaca atgcttcgtc taaaataaat gaacctgcat ggccagtagc
     9241 tccaatgagt gcaattttca taaaaaacct ccttttttat tcctgtccat tgtaagcagt
     9301 tgctgaatat tcgtcaaaga atccgatttt tgaaaagctc agtgcgcatt cctttcatgg
     9361 ttcgtgatca ttgggtatag ttaaattaag aaaactgatt gataaaaata gaagttgaag
     9421 gagataagaa atgaaagagt ggttagatcg tgccttttta ccaatgataa catctgttgt
     9481 gaatcttaga cccttaactg ctttgcgtaa tggaatgact tacacgatcc caataattat
     9541 tgttggctcg ctgtttttct tatttgctca ttttcctgtg gattacatcg ctcagtggat
     9601 agacaaaaca ggattttcta tgtattttac acaagtatac caggctacat ttgatgtgat
     9661 agccttttgg atcgtttttg ccattgcata cgcttatgca aaagaagaag acatcgatcg
     9721 cttaccagtc ggcataacag ccttgtctgc ttatctactg cttattcagc cactaaatac
     9781 tcagatagat actgtctgga caggtgctga agggatacta ggagcagtta caactgggct
     9841 catcatcgga tggggctatg cacgtttagt aagacttgcg aaagggtgga agcaaagaga
     9901 gaagatacct gaaaatgttt ggcagtcttt tacttcattg attccaatca ttgttatagt
     9961 cacttttacg cttatcggtt cagcggtttt tcaacagttg ttcagaatga ctgttgttca
    10021 aggagtatgg acatatgtcc agcttcccat tcaatcgttc atagacacgt tgtttggtgt
    10081 gcttttatta ggtttttttg ttccgttttt atggttattt ggtcttcatg gatctgcgat
    10141 ggtcaatggg ttggttagtc ccgtcttgca agcgaattca ttagcgaatg cagaaatctt
    10201 agcctcagga caagagttga cggtggcaaa tggcggacat atcgttactc agcaatttct
    10261 cgatcagttc atgacggtta cgggtgctgg attgacgtta ggggctgtat tttttatgac
    10321 ggtttttgcg aaatcgaaga aatacagaga attaggaaat cttgctcttt taccagcctt
    10381 tttcaacatc aatgaatcaa ttattttttc cacaccgatc gtgatgaacc cgatgatggc
    10441 tgttcctttt attttcgcac cggtcgtttc aggattgatt acctattccg ctttatattt
    10501 tggattcgtt cctttattta cagcagtcca agtcccttgg acaacaccgc cgatcctctc
    10561 gggtttctta gtaggcggag tgcctgcagc gattttacaa ggtatcgtgt tgtcaattag
    10621 ttttttcatt tatttcccat ttttgaaaaa aatagattct gcaaactgca aaaaggaaag
    10681 acagacaaaa aatgatggag cagcagaaaa gatgaacgac taaaaaacaa aaatactcct
    10741 gtcgctttag attgggaaaa tcaccaatct aggatgacag gagtatttat agtcatgttt
    10801 atactagccc tttttggaca gctaattgaa caagaacaac taatgtgttc attcctacgt
    10861 gtgtgatcat cgatgtccag atccgaccag ttgctttgta ctctaggctg aagaagaaac
    10921 ctaggaaaaa gtagattagc agatgaccat cattatgagc aaacgcaaac agcaaagagc
    10981 tgatcactgc tccgacccaa aaatttgaat atcgactgat gatccccagt atactgcggc
    11041 ggaatacgaa ctcttccatg atgggtccag caacagttgt cgccaaaata aaagcgggtg
    11101 cttccatgat catctggatg atattttgtg tattctcgga aggggttgct ttgccaaaca
    11161 atacagattc taacatgaca gcgatgcttt gcagaatcaa tgcgacaaag attccaacca
    11221 atccataaat aacaatcttt ggagcagaag ctttttttac cgctgattcg atcgctaagg
    11281 gttcacgttg cttaaaataa aaaatagcta ggacaacggc gcctagtata tacatgactg
    11341 cagttgctgt gatcaattca gacgtagatt ttacaagtcc tatcgaagct aggatcaagg
    11401 gtgagataaa aattagacca tagcaaaaaa tgctgaggaa gctatatttt tttaaagaca
    11461 tgtaattcct ccttttaagg gataacaagc atgcaaggca agcttatatc gattgatttc
    11521 aattatatca taatttcaat aagcagctgt cagaataaaa tttcttttct ttgatttaaa
    11581 agcttgcaaa aaagagggag aatgagtatt attaaaaatg taggttagca ctcgaaggtt
    11641 aagagtgcta acagattaaa aaaagagtta tcggagggat ttatcgtgtt aaaaccatta
    11701 ggtgatcgcg tcattatcga agtcgcaaaa gaagaagaaa aaactgtcgg aggaatcgtt
    11761 ttagcttctg ctgctaaaga gaaaccccaa actgggacag ttgttgcagt cggtgaaggc
    11821 cgtttgttag aaaacggtga aaaagttcct gccgctgtaa aagcaggcga ccaagttatg
    11881 ttcgaaaaat acgccggtac agaagtaaaa tacgaaggaa aagaatatct gatcgtcgca
    11941 ggaaaagata tcatggcgat tgttgagtaa atttggaaga taaaaaaata atcaaaaata
    12001 atcaatgagg tgaatcaatc atggcaaaag aattgaaatt tgcagaagac gcacgcgcag
    12061 ctatgttacg cggggtagat aaattagcag atacagtgaa agtaacatta ggacctaaag
    12121 gccgaaatgt cgtactagaa aaatcttacg gctctccatt gatcacaaat gatggtgtga
    12181 cgatcgcaaa agaaatcgaa ttagaagatc attttgaaaa catgggtgca aaacttgtat
    12241 cagaagtagc atcaaaaaca aatgatattg caggagacgg tacaacgaca gcaactgttc
    12301 tgacacaagc aatcgttcgc gaaggtttaa aaaacgtaac tgcaggtgct aatcctctag
    12361 gcattcgtcg cgggatcgaa ttggcaacaa aagcagcagt cgaagaattg cataatatct
    12421 caactgttgt tgattcaaaa gaagccattg cacaagtagc agctgtttct tcgggttctg
    12481 ataaagttgg acatttgatt gctgatgcaa tggaaaaagt tggaaacgac ggtgtcatca
    12541 caatcgaaga atcaaaaggg atcgaaacag aactagatgt tgtagaaggt atgcagtttg
    12601 atcgtggtta cttatcacaa tatatggtaa cagataacga caaaatggaa gctgttctag
    12661 aaaacccata tatcttgatc acagacaaaa aaatctctaa catccaagat attctgccat
    12721 tgttagaaca aatcttgcaa caatcacgtc cattgttgat tattgctgac gatgtagatg
    12781 gtgaagcatt gccaactctt gtattgaaca aaatccgtgg aacattcaac gtagtagctg
    12841 taaaagctcc tggtttcggc gatcgtcgta aagcaatgct tgaagatatc gctattttga
    12901 caggtggtac agtaattact gacgatctag gtttagaatt gaaagatgta acaatcgaaa
    12961 acttaggaaa tgcttctaaa gttgttgtag ataaagacaa tacaacgatc gtagaaggtt
    13021 ctggagaaaa agaagcaatc gaagctcgtg tgcaattgat caaaaaccaa attgctgaaa
    13081 caacttctga ttttgaccgc gaaaaattac aagaacgctt agctaaatta gcaggtggtg
    13141 tagctgttgt taaagttggt gcagctactg aaacagaatt gaaagaatta aaattacgca
    13201 ttgaagatgc attgaatgct acacgtgccg cagtagaaga aggaatggtc tcaggtggtg
    13261 gtacagctct agtgaacgtt atcagcaaag tttctgctgt agaagcagaa ggagacgtgg
    13321 caacaggtat caagatcgtt gttcgtgctt tagaagaacc aattcgtcaa atcgctgaaa
    13381 acgctggtta tgaaggatca gtgattgttg ataaattgaa gaacgtggaa ctaggcactg
    13441 gtttcaatgc tgctactgga gaatgggtaa acatggttga agcaggtatc gtagacccaa
    13501 ctaaagtaac acgttctgcc ttgcaaaatg ccgcttccgt ttctgccttg ttattaacga
    13561 cagaagcagt cgttgcagat aaaccagaac cagcagcgcc agcagctcct gcaatggatc
    13621 catcaatgat gggcggtatg atgtaagaaa aagcaacttg cttatctaca actgatcatt
    13681 agtgaaagac agataaaaag aggctggaac agaagcgttt aactccaaga aataagaaga
    13741 aattcacgga aattgctttt caaatttttg tgaatttcag cttatttccg aaggagttgc
    13801 ttctgcttcc gctgtttata cgtgtttaga gcgtgagaca aaagtgttct ttacttttgt
    13861 cccacgctct tttttgtgtg taattataat gaaaatagtt atttttaata gttataatat
    13921 tttatctatt aaaagtaaat atccattc
//
LOCUS       DACDPS010000063        13870 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.063, whole genome shotgun sequence.
ACCESSION   DACDPS010000063 DACDPS010000000
VERSION     DACDPS010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.063"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            56..1975
                     /gene="tet(M)"
                     /locus_tag="IWJ07_001984"
     CDS             56..1975
                     /gene="tet(M)"
                     /locus_tag="IWJ07_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAP9333558.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNVCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2094..2261
                     /locus_tag="IWJ07_001985"
     CDS             2094..2261
                     /locus_tag="IWJ07_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9333559.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(2321..2674)
                     /locus_tag="IWJ07_001986"
     CDS             complement(2321..2674)
                     /locus_tag="IWJ07_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9333560.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSIM
                     ESLASGINEARNIED"
     gene            3179..3601
                     /locus_tag="IWJ07_001987"
     CDS             3179..3601
                     /locus_tag="IWJ07_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9333561.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYRHRTSGLALIKKFMEEFEE"
     gene            3598..3828
                     /locus_tag="IWJ07_001988"
     CDS             3598..3828
                     /locus_tag="IWJ07_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9333562.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSMVVDEVLRGRMETRLITKILSFEIK"
     gene            4289..4492
                     /locus_tag="IWJ07_001989"
     CDS             4289..4492
                     /locus_tag="IWJ07_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAP9333563.1"
                     /translation="MKQTDIPIWERYTLTIEEASKYFRIGENKLRRLAEENKNANWLI
                     MNGNRIQIKRKQFEKIIDTLDAI"
     gene            4574..5791
                     /locus_tag="IWJ07_001990"
     CDS             4574..5791
                     /locus_tag="IWJ07_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9333564.1"
                     /translation="MSEKRRDNRGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKL
                     VATDRVPAGKRDCISLREKIAELQKDIHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNT
                     ETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSENGYAYQTINNYKRSLKASFY
                     IAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYSKNYDEILI
                     LLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVE
                     EAYQAFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNE
                     DKLPHITPHSLRHTFCTNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMK
                     RLNKEKQQERLVA"
     gene            6023..6349
                     /locus_tag="IWJ07_001991"
     CDS             6023..6349
                     /locus_tag="IWJ07_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP9333565.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            6417..7130
                     /locus_tag="IWJ07_001992"
     CDS             6417..7130
                     /locus_tag="IWJ07_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP9333566.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            7440..8291
                     /locus_tag="IWJ07_001993"
     CDS             7440..8291
                     /locus_tag="IWJ07_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9333567.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            8412..10277
                     /locus_tag="IWJ07_001994"
     CDS             8412..10277
                     /locus_tag="IWJ07_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9333568.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            10325..11734
                     /locus_tag="IWJ07_001995"
     CDS             10325..11734
                     /locus_tag="IWJ07_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAP9333569.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            11842..12543
                     /locus_tag="IWJ07_001996"
     CDS             11842..12543
                     /locus_tag="IWJ07_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9333570.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            12702..13181
                     /locus_tag="IWJ07_001997"
                     /pseudo
     CDS             12702..13181
                     /locus_tag="IWJ07_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
BASE COUNT         4699 a         2261 c         2944 g         3966 t
ORIGIN      
        1 tatttatcac tgggattttt atgccctttt gggcttttga atggaggaaa atcacatgaa
       61 aattattaat attggagttt tagctcatgt tgatgcagga aaaactacct taacagaaag
      121 cttattatat aacagtggag cgattacaga attaggaagc gtggacaaag gtacaacgag
      181 gacggataat acgcttttag aacgtcagag aggaattaca attcagacag gaataacctc
      241 ttttcagtgg gaaaatacga aggtgaacat catagacacg ccaggacata tggatttctt
      301 agcagaagta tatcgttcat tatcagtttt agatggggca attctactga tttctgcaaa
      361 agatggcgta caagcacaaa ctcgtatatt atttcatgca cttaggaaaa tggggattcc
      421 cacaatcttt tttatcaata agattgacca aaatggaatt gatttatcaa cggtttatca
      481 ggatattaaa gagaaacttt ctgccgaaat tgtaatcaaa cagaaggtag aactgtatcc
      541 taatgtgtgt gtgacgaact ttaccgaatc tgaacaatgg gatacggtaa tagagggaaa
      601 cgatgacctt ttagagaaat atatgtccgg taaatcatta gaagcattgg aactcgaaca
      661 agaggaaagc ataagatttc agaattgttc tctgttccct ctttatcatg gaagtgcaaa
      721 aagtaatata gggattgata accttataga agttattact aataaatttt attcatcaac
      781 acatcgaggt ccgtctgaac tttgcggaaa tgttttcaaa attgaatata caaaaaaaag
      841 acaacgtctt gcatatatac gcctttatag tggagtacta catttacgag attcggttag
      901 agtatcagaa aaagaaaaaa taaaagttac agaaatgtat acttcaataa atggtgaatt
      961 atgtaagatt gatagagctt attctggaga aattgttatt ttgcaaaatg agtttttgaa
     1021 gttaaatagt gttcttggag atacaaaact attgccacag agaaaaaaga ttgaaaatcc
     1081 gcaccctcta ctacaaacaa ctgttgaacc gagtaaacct gaacagagag aaatgttgct
     1141 tgatgccctt ttggaaatct cagatagtga tccgcttcta cgatattacg tggattctac
     1201 gacacatgaa attatacttt ctttcttagg gaaagtacaa atggaagtga ttagtgcact
     1261 gttgcaagaa aagtatcatg tggagataga actaaaagag cctacagtca tttatatgga
     1321 gagaccgtta aaaaatgcag aatataccat tcacatcgaa gtgccgccaa atcctttctg
     1381 ggcttccatt ggtttatctg tatcaccgct tccgttggga agtggaatgc agtatgagag
     1441 ctcggtttct cttggatact taaatcaatc atttcaaaat gcagttatgg aagggatacg
     1501 ctatggttgt gaacaaggat tgtatggttg gaatgtgacg gactgtaaaa tctgttttaa
     1561 gtatggctta tactatagcc ctgttagtac cccagcagat tttcggatgc ttgctcctat
     1621 tgtattggaa caagtcttaa aaaaagctgg aacagaattg ttagagccat atcttagttt
     1681 taaaatttat gcgccacagg aatatctttc acgagcatac aacgatgctc ctaaatattg
     1741 tgcgaacatc gtagacactc aattgaaaaa taatgaggtc attcttagtg gagaaatccc
     1801 tgctcggtgt attcaagaat atcgtagtga tttaactttc tttacaaatg gacgtagtgt
     1861 ttgtttaaca gagttaaaag ggtaccatgt tactaccggt gaacctgttt gccagccccg
     1921 tcgtccaaat agtcggatag ataaagtacg atatatgttc aataaaataa cttagtgtat
     1981 tttatgttgt tatataaata tggtttcttg ttaaataaga tgaaatattt tttaataaag
     2041 atttgaatta aagtgtaaag gaggagatag ttattataaa ctacaagtgg atattgtgtc
     2101 ctgtatgtgg aaataaaaca cgattaaaga taagggaaga tactgaatta aaaaaattcc
     2161 ccctctattg tccgaaatgc agacaagaaa atttaattga aataaagcag ttcaaagtaa
     2221 ctgtgattac agagccagac gcaaagacgc agagccgata aaatgagatt aatacaatct
     2281 cattttatcg gctctttccg ttatgtatgg attcttttaa ttagtcttcg atgtttcttg
     2341 cttcgttgat accgctggct aaagattcca ttatggatag ttctttgtct gtaaagctat
     2401 ccatgtattt ctctatctgt aatcgtcggg tgctttttac caagttatta gcaggtaaga
     2461 aaaattcatc aacggaaaca tgaagtaacg atacaaggtc ataaagaact tgtatgctgg
     2521 ggtgttgccc tttattttca atattagtta agtaccgtgg gtcaatttca atcaatgctc
     2581 ccacttgttc acgagttaaa cctcgtttca atcgagcttc tttaatggct aaaccaaagg
     2641 ctctaaaatc atatttatct tcttttttac gcatagtaga ccacctctat acattttatt
     2701 gttcctactg aattaaaaac aggtatagaa aaacgtgtta tatggtttat aggtttatat
     2761 ttaataaaaa gcactactaa acgccaataa aaaaaaccgt tatatggtag tgctatttac
     2821 gctgttaaaa tattgtatat tacttccaaa tggcggtttg ttggaggtca acgtcgccat
     2881 gaagtacatc atatacaata aatttcctta cattgggttc ttgtcaaaaa aagtcgtcta
     2941 tctgcaatag ataagtacgt ccaccaatgt ggttttataa atcatataga tagaataaca
     3001 gaagcatgta aacagagaaa taaatctgtt tatatgcttt tttggctatt cagaactttt
     3061 ttacaaagtt tatttatcag taatgcaaca aatccccctt tcacattggg actaagagtg
     3121 aaaggagata aacgagcaag gctcacttcc tttcctagac agaaaggggg tgagaaacat
     3181 gaaaccatct tcttttcaga ccacaataga aaatcagttt gactatatct gtaaacgtgc
     3241 tatggaagac gagcgaaaga attatatgct ttatctttca aggattgcaa agcgtgaggt
     3301 gtccttttcg gatgttggcg attatcttgt tagccagttt gcgacaacag ataactattc
     3361 aactgacttt cagattttta cactcaatgg gttatcagta ggcgttgaaa atgatttgtt
     3421 gagtgaagca ttacgtgagt tgccagacaa gaaacgtgaa attctactgc tgttttactt
     3481 tatggacatg agcgattcag aaattgcaga cctgttgaaa ttgaaccgtt ctactgtcta
     3541 tcggcataga accagtggac tagccttaat taaaaagttt atggaggaat ttgaagaatg
     3601 aaaacacaat atcctatgat tccctttcct ctcattgtaa aggcaacaga tggcgatacc
     3661 gaagcgatta accagattct acatcattac agagggtaca taacgaagcg ttccctacga
     3721 cttatgaaag atgaatatgg caatcaaagt atggtcgttg atgaagtctt acgtggaaga
     3781 atggaaacca gactgattac aaagattttg tcatttgaaa ttaagtaata tcctctctcc
     3841 tttcgtggaa gcgtgctaaa ccattccacg cttcccgaac agggaggttt gttattccac
     3901 caaagcatat tgagctttca atgtgttttg ataggctaac gagccattgt tctttgaaaa
     3961 ctgaataaaa gtaatcgaat acgtttcgat aagaaaagag ccaacggaac taaccgccat
     4021 gacctatctt ataaagatag cgagcgattc atgttagtga tccgagaagc aatctttagc
     4081 aggattgcct gcaacgacat tcttatcgtg ataatgatac tcccatacag tcaatagtcc
     4141 gagcgtgata aaaccgtcgc aggcaatgag tatggctaca tgagaaccat gcaggggtgg
     4201 aactcccgtg agctttgcta aagctgttcg attgctggta aaacaacttt tatgaaatcc
     4261 aaataagtga tttggaaagg aggattttat gaagcagact gacattccta tttgggaacg
     4321 ttatacccta accattgaag aagcgtcaaa atattttcgt attggcgaaa acaagctacg
     4381 acgcttggca gaggaaaata aaaatgcaaa ttggctgatt atgaatggca atcgtattca
     4441 gattaaacga aaacaatttg aaaaaattat agatacattg gacgcaatct agcgtcgcca
     4501 aagggtcttg tatatgataa aatagtatta agtcgtatca aggctctttc cataaaggaa
     4561 aggagcaaat gccatgtcag aaaaaagacg tgacaataga ggtcgaatct taaagactgg
     4621 agagagccaa cgaaaagacg gaagatactt atacaaatat atagattcat ttggagaacc
     4681 gcaatttgtt tactcgtgga aacttgtggc tacagaccga gtaccagcag gaaagcgtga
     4741 ttgtatctca cttagagaga aaatcgcaga gttacagaaa gacattcatg atggtattga
     4801 tgttgtagga aagaaaatga cactctgcca gctttacgca aaacagaacg ctcaaagacc
     4861 aaaggttaga aaaaacactg aaactggacg caaatatctt atggatattt tgaagaaaga
     4921 caagttaggt gtaagaagca ttgacagtat taagccatca gacgctaaag aatgggctat
     4981 tagaatgagt gaaaatggtt atgcttatca aaccatcaat aactacaaac gttctttaaa
     5041 ggcttcattc tatattgcta tacaagatga ttgtgttcgg aagaatccat ttgactttca
     5101 actgaaagca gttcttgatg atgatactgt ccctaagacc gtactaacag aagaacagga
     5161 agaaaaactg ttagcctttg caaaagctga taaaacctac agcaaaaatt atgatgaaat
     5221 tctgatactc ttaaaaacag gtcttcgtat ttcagagttt ggtggtttga cacttccaga
     5281 tttagatttt gagaatcgtc ttgtcaatat agaccatcag ctattgagag atactgaaat
     5341 tgggtactac attgaaacac caaagaccaa aagtggcgaa cgtcaagttc ctatggttga
     5401 agaagcctat caagcattta agcgagtgtt agcgaatcga aagaatgata agcgtgttga
     5461 gattgatgga tatagtgatt tcctctttct taatagaaag aactatccaa aagtggcaag
     5521 tgattacaac ggcatgatga aaggtcttgt taagaaatac aataagtata acgaggataa
     5581 attgccacac atcactccac atagtttgcg acatacattc tgtaccaact atgcaaatgc
     5641 aggaatgaat ccaaaggcat tacagtacat tatgggacat gctaatatag ccatgacgct
     5701 gaactattac gcacatgcaa cattcgattc tgcaatggca gaaatgaaac gcttgaataa
     5761 agagaagcaa caggagcgtc ttgttgctta gtagtacaaa tgaatttact acttatttac
     5821 cacttctgac agctaagaca tgaggaaata tgcaaagaaa cgtgaagtat cttcctacag
     5881 taaaaatact cgaaagcaca tagaataagg ctttacgagc atttaagaaa atataaaaag
     5941 ataattagaa atttatactt tgttttatgt aaaaaaatgg tatgattaat caagtaagaa
     6001 gtgattggaa gaaggcaagc aaatgaatag tatggaaaaa gtcaaccagt ttagagatga
     6061 acgtaattgg cgacaatttc ataacgaaaa agatttggct atctctattt ctttggaagc
     6121 atctgaattg ttagaacttt ttcaatggaa aacgccggaa gaagtaaaag aaacacaatt
     6181 agaacgaatc aaagaagaac tggcagatgt attgatctat tcatacatga tggcagacaa
     6241 ccttcacctg gatctagatg agatcattga agaaaaattg atcaaaaata atttgaaata
     6301 tcctgtagaa aaaagcgcag gtgtacgaaa aaaatacaca gagttgtaga aatgtgtcat
     6361 tcggctgttg gaaagagcaa aagaaaaaca gtaaaatgag tctggaggga gagaaaatga
     6421 aaaaaaaagg aaaagcaaaa agatggctag tcaatatcct cttgttcctc ttgttattag
     6481 taggactagc tttgattttc aatgaacaga tcaaggatta ttttgtcaga gagacaggag
     6541 ataaatatgc tatagcgaat gtaacaaaag aagatctgaa aaagaataac gataaagatg
     6601 tcagttttga ctttgatgcg gtagaaccaa tgacgacaga aggggtcatg aggtcgcaaa
     6661 tgagaggaac agatctacca gtgattgcta gtatcgcagt cccttcagtt tcaatcaatt
     6721 taccggtttt caaaggcttg gataatacgt cactacttta cggagcagga acattatctc
     6781 cagatcaaga aatgggaaaa ggaaattatg ccttagcaag ccatcgtgca acgaaccctg
     6841 aattgctgtt tacaccgcta gaaaatttag agatgggtgc caagatctat ttgacagatt
     6901 tagaaaatgt gtatacatac aaaacatttt ttaaagaaaa agttgctcct acagataccc
     6961 aattactgaa tgaagtcgag ggaaaagaaa ttgtcacact aattacttgt ggcgacatgg
     7021 acgccgtcac gcgactagtt gtacaaggag aactggaaag tgttacatcg ataaaagatg
     7081 cgacagatga tatgagatct gctttcaatc tagagacaaa aacattttag gatactggga
     7141 cataagtcgt tacagattaa aaacagccaa aggatatgaa tcgataagga aaaaccaatt
     7201 tgctgaaaaa aagagaaacg caaagaggtg catctctgat ttggttgttt tctagataga
     7261 cttgtgttcc agttttttta ttttttatat gaaaaggctt gcataaataa aaattcgatg
     7321 gtatgatgat tttgaataag aaatggattg ttactgtacg gcaggcaaaa ccagaattca
     7381 gctaagaaac agtaggcgaa ttctaggttt ttttattttt cttgagaggg cgaagcaaaa
     7441 tgaaaattga taaaattttg aataacaatg tcgtcatttc gaaaaatgga ttcggggaag
     7501 aagtcgtttg tatgggaaaa ggactagctt ttcaaaaaaa gattggggac gagatttcgc
     7561 cagaagcggt ccaaaaagaa tttgttttaa gagattcttt tgctaaaaac caattccagc
     7621 agctgatggc tgatattccg gcagaggaga tcgaatgggt caaacaagta gtcgaactgg
     7681 ctgaagataa actaaaagtt gaattttcgc ctaatattta tctgacattg accgatcatc
     7741 ttcattatgc gatcacacga gcaaaagaaa atatccaact gcctaatcca ttattatttg
     7801 aaacaaaaaa attttatccg aaagaatatc aagcagcgaa agaggctctt ttgttcgtga
     7861 aacaaaagac gggtgtggaa ctttctgagg atgaagccgg ttttattgcc tttcattttg
     7921 tgaatagcca acaaggaaac gaagacatgc aagtgacgat gaccgctacc acaatggtaa
     7981 gagatatact gagtatcatc agtaaattct tcggcatcgt gttcgatgaa gaatctttga
     8041 attatcagcg tatcattact catctgcagt tttttgccca gcgttatctc aaaggggaag
     8101 gatctgacga gcaagatgaa tttctctacg cattagttca aggaaagtat ccaaaagcgt
     8161 ttcattgtgt cgaacggatc aatgagtatc ttcgacaaac gcaaaaacag gaaatgggga
     8221 tcgcagagca gatttattta acgattcata ttcaaagagt agtaagtgag aaaaaaagca
     8281 tccaatcata aggattgtta ctgtatggca ggcaaaacct ggattgaaaa aataggttca
     8341 cgaatgtttg tgactgtttt tttcaatctg ggtttttatt ttttaaataa aaaagaaaga
     8401 aggaaaaaac aatggataat caagcagttg gtcgtcgtgt atgggaagcg gtaggaggac
     8461 aaaagaatgt caaaagttta gtacattgtg ctacgcggtt gcgattcaga ttaaaagatg
     8521 aatctttagc agatacgcaa aagttaaaag aagacccgga cgtgatccaa gtcgtacaaa
     8581 gtggcggtca gtatcaagta gtgattggca gtaatgtagc agatgtttat caatcgatcg
     8641 tggatgagga aggtcttgca gtttcggagg atggaaaaga agagtccaaa aatattttga
     8701 atcgtctgat cgatattatc tctagtatat ttacaccttt tctaggggcg atggcagccg
     8761 ccggggtatt gaaaggattc ttatcattgg caacggtgat gggctggctg acagctgata
     8821 gcggtactta tcaaatattg tttgctgcag cagatggtgt ctttaccttc ttacctgtca
     8881 tgcttgcttt tacagcagct aaaaagttca aagccaacca gtttttagca gtagcaattg
     8941 ctatggcact tgtgtatcca gctatcacag cagttgctgg tgcgggagaa gctatcagtt
     9001 tctttgggat cccagtgatt ctttcaccat ctggttatac ttcttctgtc ataccaatca
     9061 ttctagccgt atgggttcaa agtaaattag agccattcgt aaaaaaagtg attccgcaat
     9121 ttctgcaaat gattttagtt ccgcttgtcg ttttagtagt gatggttcca ttaacatttc
     9181 ttgctttagg accaatcggg acggttgccg gtaatgcttt aggcggtctc ttcaactcga
     9241 tttatggatt tagtccaatc gtagcaggtc taatcatggg aagtttatgg caagtattcg
     9301 tgatgttcgg catgcattgg ggattcgtac cgatcatgtt tttgaatatc gaacaatatg
     9361 ggtttgatgt attgatgcca atgcttcttc cagccatctt ggcacaaggt ggggctgctt
     9421 tagctgtagc attgcgaaca aaagatacga aacttcgagc attagggatt tcttctacag
     9481 tgacttcgtt atttggaatc actgaaccaa ctgtgtatgg tgtgacatta cctttgaaga
     9541 aaccattcat tgccgcttgt atttctggag gtattggtgg agctattatc ggattttccg
     9601 gagtaaaagc tttctccagc agtttggtca gcttattgac gattccgact ttcattaata
     9661 cggtggatgg tgtagagtca aatgtgacag tagcagtaat agctacagga attgcttttg
     9721 ttcttgcatt cgtaggtaca ttgattttag gatttgacga acaaacacaa gacaatcaat
     9781 tagaaaacaa acatgcgaat gcaggagaac cgattacttc agcacggcat accttaaaaa
     9841 gtcctttaac gggtaaagta ctgccattga gtgaagtacc agaccaagtt ttttcttctg
     9901 gcgtgatggg aaaaggtatc gctattgatc cagaagtagg agaactagtt gcacctgcag
     9961 acggagagat cacgactatt tttccaactg gacatgctgt agggatcaca acgactgacg
    10021 gagcggagat tttgattcat atcgggatgg atacagtaga attaaatggg aatggctttg
    10081 agatcttagt caaacaaggg gatctcgtca aagcaggaga tttattgatt cgttttgaca
    10141 tcgaagcaat tagagcagct ggctatagcg taatcactcc agttgtcatt acgaatactg
    10201 atgcatttgc cgatatactt gaattagacc aaaaagagat tattgcaaac gaagatgttc
    10261 tggcaattgt aaaataagga ggaaatcagg atgcaatcac gtttttcaga aaaattttta
    10321 tggggtggag caactgctgc caaatcaatt agaaggggct tatgatcgag acggaaaagg
    10381 actatccgtt gcagatgcta tgcctggagg taagcagcgc tttgcgatta tcggcagtga
    10441 agaatttgac tggacgattg atcaagaaaa atatatttac ccgaatcatc gaggaatcga
    10501 tcattacgat cgtttcaaag aagatcttgc cttgtttgcg gaaatgggat tcaaatgtta
    10561 tcgtttttcg attgcctgga cacgaatttt tccaaatgga gacgaaggta ctcctaacga
    10621 agcgggatta gaattttatg atcagctgat tgatgagtgc ttaaaatatg atattgaacc
    10681 tgtgatcacg atttctcatt acgaaatgcc tcttcatttg gcaaaagaat acggtggctg
    10741 gaaaaatcga aaactgatcg acttctatga acgatttgct cagacagtat tagagagata
    10801 cagcagtaaa gtcaaatatt ggatgacttt caatgaaatc aattcggcct ttcactttcc
    10861 agctttaagt caaggattag taaaaagcaa tggagcagga gagtaccaaa atattttcca
    10921 agcatggcat aatcaattcg ttgcaagtag caaagccgta aaaatcggcc acgaattgcg
    10981 ttcggatatc caaatcggct gcatgattat ctatgcaaca acttacagca tcgatgccaa
    11041 tccagtcaat caggcagcaa caatgatcca aaaccaagaa ttcaattttt tctgcacgga
    11101 tgtccaagta cgtggagaat accctgcata cacagcgcgt acccataaaa aatatggagt
    11161 cgatccggaa aaattagagc aaacggaaaa agacttccag ttgctaaaag aatatccagt
    11221 ggactacatc ggatttagtt attatatgtc tacggcaatc aatgagactg atccacaagc
    11281 agcaacttca gaagggaact tgctgggagg agtaaaaaat ccgtttcttg aggcaagtga
    11341 atggggctgg cagatcgatc cagaagggct gagaatcgca ttgaatgaat tatacaatcg
    11401 ttaccagaaa ccattattca ttgttgaaaa cggtcttgga gccattgatc atatggaaga
    11461 ggatggcacg attattgatg attatcggat cgattattta cgtcgtcata tcgaagcgat
    11521 ggctaatgca gtagcagacg gtgtagattt gatgggctat acgccttggg gatgtatcga
    11581 tcttgtcagt gcatcaacag gagaaatgag taaacgttac ggctttatct atgtggatct
    11641 agacgatgaa ggaaatggga cactgaatcg atcaaagaaa aaatcttttg attggtataa
    11701 agaagtaatt gcctcaaatg gcgtaaactt gtaattaaag cagctgaatg aaaaagaaca
    11761 cacttctcac atgtgatttg tgttcttttt ttatttttta tgtgctattc ttttagtaat
    11821 agtaaagcaa agggtgacgt tatgaaaagg acacgtgtac tatacttgga agtcgcagat
    11881 aaaattaaag aagatatttt ctcaggaaaa tatccagtcg gttcgatgtt gccgacagaa
    11941 agtgaattag aagaactgtt tcaagtcagc aaaatcactg tccgaaaggc aatcgaactg
    12001 ctggcatcag atgaatatgt cgcaaagaaa agtggtcgtg ggacgacggt tctaagcaat
    12061 cgtccttaca atcgattatc caaagcggca agctttacgc aaatcttgga gaactcccga
    12121 tatgaagtcc ggaaagaaac gctggatttg caattggtcc atataaagaa ggaagatcct
    12181 ttgttttcct ttttcggtga gcaagctgtt tgttttaagc gcttatatta tttgaacaat
    12241 cagccttata tttattttgt tcattatctt ccacaagaaa tgatgcaatt cagcagagaa
    12301 gaatttggca aagaatcttt gtatcgtttg ctgacgaaaa aaggatatac tattgactca
    12361 ttttctgatg atttctcggc agtattttta acagaagaag aacaaggaat tctgaaaaca
    12421 gacgcacaga tcggcatcaa acggatacgg aaatcgtcta cgcaaattgg ggaaatgatt
    12481 gaatattcgg aggcggtcta ccatacggag ctgcatcctt accatattga ttatgaaaca
    12541 taaataaaaa aagaggttgt gaaaaagctg ccctgcatca agtaataaga acagccaaac
    12601 aaaaatagct tctcatattt ttcgtttggt taggcttatt accgcagata cacggctgtt
    12661 tctggattac catttattct ggataagggg gtgtggcacg attttcagaa ggaatctatg
    12721 aaccaatcac tgtattgatt ttagcttcga tggtaacccc attcggcttg acgatcgctt
    12781 atttcttagg gaaaatcatt cgtaaaaata tactcaatcg tcaagaaatc gatacattga
    12841 agaccgcttt tcctatgggg atctgtcaga tcacagaggg atgtttccct atcgtattga
    12901 acgacttggc acgcaatgtc atagcaacgg gagtcggtgg gacagtaggt ggcggattaa
    12961 gcatgttttg gggcgcagat agccgtattc cggcatcggg aatgtttgca gtcgctacga
    13021 tgaccagacc atgggcattc attggtgcat tgttagctgg ttcttttgtc acaggaataa
    13081 ccattttatt attgaaaaaa ccagtagatc cgaatgcaga aatcatccaa gaagaaaaag
    13141 aggaagaaga catatcttgg gatgatttga caattagtta ggagaggcag aatggattac
    13201 aagaaaaaag tacaatagat gtaccttgag acaggaatgg tatgaaagaa tcagagttag
    13261 aaagaatcga atatacagat tttggtttag gagcgatcga aactgtccag agcatcggca
    13321 ccctccttga aatgacgatg aaggaaaaat gagaaccttt cgttgtcgaa agggtctggt
    13381 ataccttatg tcgaaggaaa ggaaacaaaa aaactttcct gtactattcc aaaaaggaaa
    13441 gaaagttatt atacagtaaa aaaagaaatt attcttcatg ctagagatca gtacacgttc
    13501 gagcccaata tcaaacacta gttccaggca ggcgcaacag gtgcggttat cactgaattt
    13561 tcttccagca gtgacgattc aagtgatatt tttacaaatc cagctattta tcgttaacaa
    13621 aaagattttt aggagaagct gatgaacaaa aatgatcaaa ttttgatcgt ttagttcgtc
    13681 ggctttttta tttagagaaa catcatttat cagtgaaaca taacacgaga acaaaatgat
    13741 tttttcgtta ataaaagaaa caagtatttg ttgtttaaaa taatacacgt tattggcata
    13801 taggacgaaa tcccagtatc ttctataagt aagtcatttc atatcgatga aaacacgtta
    13861 tttatttttg
//
LOCUS       DACDPS010000064        13747 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.064, whole genome shotgun sequence.
ACCESSION   DACDPS010000064 DACDPS010000000
VERSION     DACDPS010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.064"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..863
                     /locus_tag="IWJ07_001998"
     CDS             <1..863
                     /locus_tag="IWJ07_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9333571.1"
                     /translation="MSQMMDFLSIFTDGMVAGSRILKIMDTEEYAPQQNPGADQMITS
                     GKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGSGKSSIINVLMRFYEFYEG
                     QILIDDHDIREYPMEELRKKMGMVLQDAFLFYGDIAGNIRMMNPAITDEQIRHAAEFV
                     QANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVTDPKILVLDEATANIDTET
                     EGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGHIVEHGNHETLLAQNGLYA
                     DMYKLQSSGI"
     gene            1189..2040
                     /locus_tag="IWJ07_001999"
     CDS             1189..2040
                     /locus_tag="IWJ07_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9333572.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEDLQVHLNDSVYIALTDHLY
                     TAIERQKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSIHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFSCIEKIADFLTGAFDYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            2137..4017
                     /locus_tag="IWJ07_002000"
     CDS             2137..4017
                     /locus_tag="IWJ07_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9333573.1"
                     /translation="MGKYQELAENIVKNIGGKENIRSLTHCVTRLRFKLKEENKAKDD
                     VLKKMEGIVTIMKSGGQYQVVIGNHVPDVYAEVLTVAGISDEAEEEVSSSSNLFNRLI
                     DILSGCFQPFLGALAAAGMVKGLNALFLFLKFYTEGSGTYIMLNGIGDAIFYFMPVIL
                     GYTAAKKFHLNPMVGLVIGAALCYPTVQGAALQSGFPDGSPAPYSLLGLPAYETFLGI
                     PWVGSSYTSSVVPVIFIIAFAGQVQKIAKKWIPAVVQTFLVPFSVLLIALPAGFLIIG
                     PIVSILTDLLSTGFQALMDFSPAVYGAVLGFLWQVLVIFGLHWSIIPLAIMQLAQNGW
                     SQMLVGVYGASFAQTAVVLAMYFKFKDQKMKALCMPAVISGIFGVTETAIYGITLPKK
                     KPFIYSMIGGAVSGIYLMASGTISYTMGGLGIFGVVNYITETDASGMFQAFIGIAIAS
                     VIGFSLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGRGI
                     LIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESLVQ
                     QGERVKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGETVGPDDALITA
                     LT"
     gene            complement(4057..4959)
                     /locus_tag="IWJ07_002001"
     CDS             complement(4057..4959)
                     /locus_tag="IWJ07_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9333574.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNDVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            5190..6593
                     /locus_tag="IWJ07_002002"
     CDS             5190..6593
                     /locus_tag="IWJ07_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP9333575.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            6593..8026
                     /gene="ascB"
                     /locus_tag="IWJ07_002003"
     CDS             6593..8026
                     /gene="ascB"
                     /locus_tag="IWJ07_002003"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP9333576.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDVFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQVKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            8171..9097
                     /gene="trxB"
                     /locus_tag="IWJ07_002004"
     CDS             8171..9097
                     /gene="trxB"
                     /locus_tag="IWJ07_002004"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAP9333577.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            9311..11038
                     /locus_tag="IWJ07_002005"
     CDS             9311..11038
                     /locus_tag="IWJ07_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAP9333578.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEEAKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            11110..11403
                     /locus_tag="IWJ07_002006"
     CDS             11110..11403
                     /locus_tag="IWJ07_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP9333579.1"
                     /translation="MPDTQKSKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(11495..12355)
                     /locus_tag="IWJ07_002007"
     CDS             complement(11495..12355)
                     /locus_tag="IWJ07_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAP9333580.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLKQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            12447..12875
                     /locus_tag="IWJ07_002008"
     CDS             12447..12875
                     /locus_tag="IWJ07_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP9333581.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            13037..13213
                     /locus_tag="IWJ07_002009"
     CDS             13037..13213
                     /locus_tag="IWJ07_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAP9333582.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            13240..13686
                     /locus_tag="IWJ07_002010"
     CDS             13240..13686
                     /locus_tag="IWJ07_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAP9333583.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         4498 a         2349 c         3074 g         3826 t
ORIGIN      
        1 caatgtctca aatgatggac tttttgagca tcttcacaga tgggatggtt gctggtagtc
       61 gtatcttgaa gattatggat acagaagaat atgcaccaca acaaaaccct ggagcagatc
      121 aaatgattac ttcaggtaag attgagtttc gcaatgtaag cttttcttat gatggagagc
      181 acaatgtatt aaacaatatc tcttttgttg ctaatccggg agagaccgtt gcattagtgg
      241 gacacaccgg aagtgggaaa agctcgatca tcaatgtgtt gatgcggttt tacgaatttt
      301 atgaaggtca gatattgatt gatgatcatg acattcgaga atatccaatg gaagagttac
      361 gtaagaaaat ggggatggtc ttgcaagatg ctttcttgtt ttatggggat atcgcaggaa
      421 atattcggat gatgaatcca gctatcacag acgaacagat acgccatgcg gctgagtttg
      481 ttcaagcaaa caaattcatc gatgaattac caaaaggata tcactcaaaa gtgatcgaac
      541 atggtgccag ctattctagt ggacaacgac agctgatcac ttttgcgcga acaatcgtga
      601 ctgatcctaa aattctagta ttggatgaag caacagcaaa tatcgatact gaaacagaag
      661 gattgatcca agaaggattg gcgaaaatgc gacaaggacg gacaacgatt gcgattgccc
      721 atcggctgtc aacgattcga gatgcagaat tgattttagt tttagacaaa ggtcatatag
      781 tagaacacgg gaatcatgaa acacttctag cgcaaaatgg tttgtatgca gatatgtaca
      841 aactgcaaag cagtggtata taaaagatgt tgtgacagta gcattttgac ctaagcacta
      901 gagtgaaaat aaaacatagc ttcccatatt tttcatctgt gagagttagt gtcgtagtta
      961 ccagatttaa aaaagacgac gtcagctagt cagaagtgcc taatagcccc taagaaagcg
     1021 ttgacaaaat cgtttgcttt cgataataca aagaaaacga tacaacttgg atagtgattg
     1081 cctaatgcaa gctaaacctg atgagtctcc catggtttca tggacggctt tttgggtttt
     1141 tctttttgtt ttggatacag ccctatatat catggaaagg agcaaaacat gctgattgag
     1201 aaagtactga ataacaacgt cgtaataacc agagatgaaa gaaatcagga aatgatcgtg
     1261 atgggaaaag gtttggcatt caaaaagaaa gtgggtgacc cgatttttga ctcatgcatc
     1321 gacaaaaaat ttcagttgac aaatgacgga ctttcacaac agttccaaga attggcacaa
     1381 gcgattcctc ttgagtactt agaaatttct tgtgaaatca ttgaatatac aaaagaggat
     1441 ctacaagtcc atttgaatga ttctgtgtat attgcattga cggatcattt gtatacagct
     1501 atcgaacgtc aaaaaatggg gatctttgtg ccaaatgtat tgctgtatga tatcaaacaa
     1561 ctgtttccta aagagtatgc cattgggaaa aaaacagtga agaaaatctc ggataaatac
     1621 cacatcaagt tatcagaaga cgaagccgga tttatcgcat tgcatttagt caatgcacaa
     1681 ggcattcaaa gccagcaaga caatacctac ctgtttacca caacaataca ggatatttta
     1741 aatatcgtcc gctattacta taagattaca tttgatgaag attcaatcca tttttttcga
     1801 ttcgtcacac acctacgttt ttttctgaca cgcgtcctag atggagagaa atcagaagtg
     1861 ggagaagttt ctgaggaatt gttccaggtt attcaaaaaa aatataagga cacgtttagt
     1921 tgcattgaga aaatagcaga ctttttaacc ggtgctttcg actaccagat gtccacggat
     1981 gaaaaaatgt atttaacgat ccatactgcg cgtttagtgc atgagaatcg ttgtaaataa
     2041 ataaaaaagg aggacaaatt aacaagaacg tatgaaaata agggtagtga cattcagttg
     2101 gctaaacctt ccactaatta attggaggga atagaaatgg gaaaatacca ggaattagca
     2161 gaaaacatcg taaagaatat cggaggaaaa gaaaatatcc gtagcttgac acactgtgtg
     2221 acgcgacttc gtttcaaact aaaggaggaa aataaagcaa aggatgatgt tttgaaaaag
     2281 atggaaggga tagtcaccat catgaaaagc ggcgggcaat atcaagttgt aatcgggaat
     2341 catgtgccag atgtctatgc agaagtactg acagtagccg ggatttccga tgaagcagaa
     2401 gaagaggttt cttcaagtag taatctgttc aatcgattaa tcgacatatt aagtggctgt
     2461 ttccagcctt ttttaggtgc tttggctgca gcgggaatgg tcaaaggatt aaatgctttg
     2521 ttcttgtttc tcaaatttta tactgaagga tcgggtacgt acatcatgct gaatgggata
     2581 ggggatgcaa ttttttattt catgccagtg atcttagggt acacagcagc gaaaaaattt
     2641 catctgaatc cgatggtggg gctcgtgatt ggtgcagcac tttgttatcc gactgtacaa
     2701 ggagcagcac tacaaagtgg tttccctgat ggcagtcctg cgccttatag cttgctgggt
     2761 cttccggctt atgaaacatt tttgggtatc ccatgggtgg gcagcagtta taccagtagc
     2821 gtcgtacctg ttatcttcat tatcgcattt gccggacaag tccaaaaaat tgccaagaaa
     2881 tggattcctg cagtcgtcca gacctttttg gttccttttt ctgtgttatt aatcgcactt
     2941 cctgctggct ttttgattat cggtccgatt gtcagtatac tgacagatct attaagcacg
     3001 ggattccaag cattgatgga cttttctcca gctgtgtatg gtgcagttct agggtttctt
     3061 tggcaagtac tggtgatttt cggacttcat tggagtatta tccctcttgc gattatgcag
     3121 ctagcgcaaa atgggtggag tcagatgtta gtaggcgttt atggcgcgag ttttgcacag
     3181 acagcagtgg tattggcgat gtatttcaag ttcaaggatc aaaaaatgaa ggcgctttgc
     3241 atgccagctg tgatttcagg aatcttcggc gtaacagaaa cggctattta tggaatcacc
     3301 ttaccaaaga agaagccatt tatttactcg atgattggtg gtgctgtttc aggaatctat
     3361 ttgatggcat ctggaacaat cagctacaca atgggcgggt tagggatttt tggggtggta
     3421 aactacatca cggaaacaga cgccagtggt atgtttcaag cgtttatcgg cattgctatt
     3481 gcttcggtaa tcgggttttc gctgactttt ttcttttgga aggatgaagc aggtgaaaca
     3541 gatgatatac agaaaaaatc aggtaaagaa attgtaaaaa caccgataca aggacaaatc
     3601 gctccactaa atgcagcaaa ggatgcagct tttgcacaag gaacactagg tcgaggaatc
     3661 ttgatctacc ctgaaaaagg agaagtacgt gccccttttg acggaacagt catgaccctg
     3721 ttcccgacga aacatgcaat tggaatggtt tctgaaacag ggctggaatt gctgattcat
     3781 gtcggattgg atactgtgca gttggaaggg aaatattttg aaagcttagt gcagcaagga
     3841 gaaagagtga aacaaggcga tctgttgcta cgcttcgaca aaaagaaaat cgaaaaggaa
     3901 ggatacagtt tagaaacacc tgtgatcgtc accaattaca cggattattt ggatattttg
     3961 gaaaaccaag gggagacggt cgggccagac gatgcactta tcaccgcact gacctgaacg
     4021 gttaggtgaa gaatggacta ggcttaacta ttctttttat tccacttctt tattttcaaa
     4081 attgaaaggc tctttcatga tggctgagct gatagggcgt ttatcgaatt tttgaccgac
     4141 agaaattaga tggactaaat tctgttgata attttctgca aaaacacaac tatataacac
     4201 gtctagaaga taacaaattg atgtattgat cgtaaaatta ccaatttttg aatacagtcg
     4261 ctcgcgtgtc gtaagtcgca aagcggctgt actcattgca gatagcgttg aatcaccgat
     4321 actggtcaaa gctaaaattg gaacatcatt ttgtttcaat atttctgcgg tacgaaggat
     4381 cacattgttt tcacccgtat aagaaatcag aatcccgcac atatcactcg tgcaattgta
     4441 cgcttcatag aacgattcac caaaagtggt gacaatcgtg acatccattt tgatgcgctt
     4501 cattttcagc atgaaatctt gagaaatcaa agtattcgag ttactggcga aaatcacgat
     4561 tttttttgct tgaagcatta gttgtttggc tttttgcagt tggtcatgat gaatgagaga
     4621 aagtgtatca tgaatcgtca tctgttctaa agaagccatt ttatttgcga tcgtcataat
     4681 accgtctgtg gtggaaaaag gaagattcgc atctacgtct tgaaaattcc cttcaagata
     4741 ggctgtttca gctaaaaaag cattacgaaa actgacccag ccatcaaaac ccattttttt
     4801 tgctacacgg atgagtgtag acgggtggac atagcatcgc tcagcgatct cttggatggt
     4861 catttcgcca agtgctggag aatcgcttaa gagaaactgg atcacttcat tttctgaagg
     4921 agaaaaggag atttctttga ttttttcacg taataacatg tagttacctt cttcttattt
     4981 ttttacgtaa attataactt ttttgaaaat gttttgtaaa cggatttata ctatataact
     5041 taaaatagac tttttttgac aagatattta caaaaatcca ctagaaaaca gtaacttttg
     5101 taaatgttac tggaaagaaa cggatttcta ttgtttgttt tgtttacaaa aaagataatg
     5161 aaagtgtaaa aaagtaaagg agtgagaaca tggcaaaagg aatcaaaatc gctacgattg
     5221 gtggaggcag cagttacacc cctgagttga tggaaggatt tatcaaaaga tatgaggaat
     5281 taccgatacg agaaatctgg ctcgtagatg tagaagaagg aaaagaaaag cttgagatcg
     5341 ttagtcagat gtctcagcgt atgtgggatg cttctccgta tgaagtcaaa atctatgcaa
     5401 ctctggatcg ggaagaagca ttgaaagatg cggattttgt tacgactcag tttcgtgtcg
     5461 gtcaattaga tgcacgaatc aaagacgagc ggatccctag ttattatggg atgattgggc
     5521 aagaaacaaa tggtgctggc gggatattca aagcttttcg taccgttcca gtggtcttgg
     5581 caatcgttga agacatgaag cgtttatgtc caaatgcttg gctgatcaat ttcgcgaacc
     5641 caagtgggat ggtgacagaa gcagttatca gatatggcaa atgggataaa gtgattggat
     5701 tatgcaatgt gccagtaatg gcgatgatga cagaaccagc aatgcttggc aagaaaaaag
     5761 aagagctgat ctacaagttt gccggtctaa atcatttcca ttggcacaaa gtagcagata
     5821 ataaaggaaa tgaccgcacg gcagaattga ttgatcgttt atacgatgaa gacaatggat
     5881 tgccgaaaaa tatctttgat atcccgtttt tcaaagaaca attaaaacaa atgaatatga
     5941 tcccttgtgg ctaccaccgc tattactacc gtgaagaaga aatgctggaa catgctttag
     6001 aagaatatca aacgattggt acaagagcgc agcaagtgaa agaaacagaa gcagagctat
     6061 ttgaattata caaagatcca gaattagacc ataaacctga acaattgcaa aaacgaggtg
     6121 gcgcttatta ttcagacgct gcctgtgaaa cgattgcttc aatctatgga aataaagaaa
     6181 cacagatcgt ggtatctaca aagaatgatg gagctgttcc tgacctgccg gcagattgtg
     6241 tagtagaagt cactgcctat gttggcgcac aaggagcgag aaatgtagcg tttggttctc
     6301 ttcctgctgc tgaaagaggc tggttgcaag taatgaagaa tatggaatta ctgacgatcg
     6361 aagcagctgt gactggtgat tatgggacag ctttacaggc gtttacgatc aatccgctta
     6421 tacgatcagg aaagacagca aaacggatca tggatgagtt gtttattgca caccagcagt
     6481 atttaccgca attcaaggaa acaatcagcc gattaaaaga agaaggaatc gaagttcaag
     6541 atgaactggc aagagaatta tcaggtaata cacatataat ggaggaagta taatgggatt
     6601 cagaaaagat tttttatggg gcggtgctac agcagccaat caatgtgagg gcggttatga
     6661 tgaaggtgga cgcggactag caaacgtaga tgttgtgcca atcggaaaag accgttttcc
     6721 tgttatcacc ggaaagaaaa aaatgtttga atttgatgag gatcattttt atccagcaaa
     6781 aattgcaatc gatatgtatc atcgttataa agaagatatt gctttgtttg cagaaatggg
     6841 gttcaagact tatcgaatgt ccatcgcctg gagtcgtatt tttccaatgg gggatgaaaa
     6901 agagccgaat gaagctggct tgaaatttta tgaggatgtc tttaaagaat gccaaaagca
     6961 cggaatcgag ccacttgtga cgattacgca ttttgattgt ccaatgcatt tgatcaaaga
     7021 atacggtgcg tggcgttcca gagaactcgt tggtttttat gaaaatctgt gtcgagtcat
     7081 cttcaatcga tataaaggac ttgtcaaata ctggctgaca ttcaatgaga tcaatatgat
     7141 ccttcatgca ccattcatgg gagcaggact gtactttgaa gaaggagaaa acgaagaaca
     7201 agtcaaatat caagcggcac atcatgagtt ggttgcaagt gccatcgcaa cgaagattgc
     7261 tcatgaagta gatccggaga acaaagtagg ctgtatgctg gcagcaggag catattatcc
     7321 atacacttgc aatcctgaag atgtttgggc ttctagaaaa tcagatcgag aaaattattt
     7381 cttcattgat gttcagtcac gaggagaata cccagcttat gcattgaaag aaatggaacg
     7441 aaatggcatt cagatcgaaa tggaaccagg ggatgccgca ttgctgaaag aacatactgt
     7501 tgattttatc tctttttctt attattcttc ccgagtttcg actaccgaca acgaactctt
     7561 ggaacagact gcaggaaata tctttgcttc tgtcaaaaat ccatatctgc aagcaagtga
     7621 atgggggtgg caaattgatc cattaggctt gagaataacg atgaatgata tttatgatcg
     7681 ttatcaaaaa ccattattta ttgtagaaaa cggcttaggt gcagtcgata cgccagatga
     7741 gaatggttat gtggaagatg attaccgaat cgattacctg gcagcgcata tccaggcgat
     7801 gaaggatgca gtagaactag acggagtcga tctattaggc tatacgactt ggggatgtat
     7861 tgatttagta tcagctggta ccggtgagat gaagaaaaga tacggattca tttatgtcga
     7921 tcgtgataac caaggaaatg gcacattagc ccgttcaaag aaaaaatcat tcaactggta
     7981 taaaaaagta attgattcaa atggagaaaa tctttcgaac gattgatcaa tgatttataa
     8041 aagtctattg tttttcagcg gatttttcga gtataataaa gctattcgag aaacgcaagc
     8101 agaacaatag actttttttc gcattggctg taaagtatgc ttacttgatt aagtaaagga
     8161 gaatctacat atgtatgatg taatcgttat tggcgcaggt cctgctggga tgactgctgc
     8221 tttatatgct tcacgttcaa acttatccgt attgatgatc gaacaaggtg cccctggcgg
     8281 ccaaatgaac aatacggcag aaatcgaaaa ttatcctgga ttcgattcga tcatgggccc
     8341 cgatctagct tataagatgt atgaaagtgt ctcaagattt gggacagaaa atgcttatgg
     8401 aatcgttcaa gatatcaagg atcacggcag ccataaagaa gtcatctgtg aagataaaag
     8461 ttacgaagga aaaacagtca ttatcgcaac aggctgtatc caccgtaaat taggcgtacc
     8521 tggcgaggaa gaatttgctg gacgaggcgt ttcttactgt gcagtctgtg atggtgcgtt
     8581 tttccgtaac aaacgtctag tagtcatcgg gggtggggac tcagcagtag aagaagccat
     8641 ctatttgaca caatttgctt ctgaagtagt gatcgtccac cgtagagatg aattacgtgc
     8701 acaaaagatc atccaagacc gtgcatttgc aaatgaaaaa atttccttta tgtgggatac
     8761 agtcgttgac gaaatcgttg gaaacgaaat ggtagtgaca ggtgtgaaag cacgtaatgt
     8821 caaaaccggc aatgaatttg atttagcagc agacggagta ttcatctatg ttggactgga
     8881 tccattaact gcacctttta gagaagcagg tttgacgaac gaagctggct ggattccaac
     8941 agacaacgaa atgaaaacga atatgccagg cgtttttgcc atcggagatg ttcgtgaaaa
     9001 agatttgcga caaatcacta cagctgttgg tgacggcggt attgctggac aacaagtgta
     9061 taaatacttg gaatctttag aagaaacaac tgcataagtt taaaaagagt agaagaacgg
     9121 gttcaattgt tcttttactc ttttttttcg cgtgaaattt actgaatctg atttatgcga
     9181 acacgtccaa caccttttga taaaagaatc ctttcttgat ttaagcgttt tttggtcctc
     9241 atactttcat atctaataag tgaatgctat acttattaag aaccatattt cagaaagagg
     9301 tgttattttc atgtcttggg aacaagtgta tgaacaatgg gtacatgaag aaaatttaga
     9361 agaaaactta aaaaaacaat taaatgatct gggtgaagat cctgaaaaac ttgaagatgc
     9421 tttttatgca ccgttggaat tcggtactgc aggcatgcgg ggaatcttag gacctgggat
     9481 caatcgaatg aacatctata cggtaagaca agcaacagaa ggactagcac gtttcatgga
     9541 tacacaaggt gaagaagcaa aacgccgagg tgtcgctatt gcttatgact cacgccatca
     9601 gtcaccagaa tttgcaatgg aagcagccaa aacactggca catcacaata tcccgtctta
     9661 tgtattcgaa agtctgcgtc caacaccaga gctttctttt gcagttcgtc atttgaagac
     9721 gtttgcggga atcatggtaa ctgcatctca caacccagcg gcttacaatg gatataaagt
     9781 ctacggagaa gatggcggac aaatgcctcc tgcagacgca gatgcattga cgaaatatgt
     9841 ccgtgaagtc agcaatccgc taaaagtcga agtattatct gacgaagaag ctaaacatag
     9901 cgggttgatc acgatcatcg gagaagaagt ggacgctgct tatctagaag aaatcaaagc
     9961 agtgacgatt gatcgtgaat tggtagaaac aatggggaaa gatttgaaac ttgtgtacac
    10021 gccacttcat ggaacaggga aaatgttggg cgaacgtgcg ttgaaacaag ctggattcga
    10081 acaattcgta ctagttcccg aacaagctgt ggcagatcca gatttcacta cggtcaaatc
    10141 gccaaatcca gaagaacatt cagcttttga atatgctata cgacttggag aaaaagaagg
    10201 agctgacttg ctgattgcaa cagatcctga tgcagaccgt ttaggcgcag cggttcgttt
    10261 gccagatggt tcttatgaag tattgactgg gaaccaaatc ggggcattgt ttgctcaata
    10321 tattttggaa gcacatcaat caagaggcac tttaccagaa aacgcagcgg tattgaaatc
    10381 aatcgtttcc agcgaattgc ctacagcaat tgcggaacac tacggtgcta ccatgttcaa
    10441 tgtgttgaca gggtttaaat tcattgctga aaaaatccaa caattcgaag aagaccattc
    10501 acatactttt atgtttggat tcgaagaaag ttatggttat ttagttaaac cttttgttcg
    10561 cgataaagac gcgatccaag cattgctttt attagcagaa gtagcagctt actataaaaa
    10621 acaaggcaaa acgttgtatg acggcttgca aacgattttt gaaacctatg gctattttgc
    10681 agaaaaaacg atttctgtga caatgagcgg acaagaaggt tctgcgaaaa tctctgcttt
    10741 gatggaaaaa ttccgtaaag aagcaccgac agaatttgcc ggagcaaaag ttattcaaac
    10801 agaagacttt aaagaattga cgagaacaga ccgtgaagga aacgtagaaa aactaacaac
    10861 accgccatcc gacgtattga aatatacatt ggaagaaaat ggctggattg ctatccgccc
    10921 ttcaggaact gagccgaaaa tcaagtttta tattggtgta aaaggaagct ctacagaaga
    10981 cgcagccgaa aaagtagcct cttatgaagc gattatcaaa aagatgacag aagaataaaa
    11041 aacttgagac tgatggaaaa agtggtaaac tttagaagtc agtctttttt caagttggag
    11101 gtgaatggat tgccagacac gcaaaaatca aaagtagaga aagtcagtca attattcaaa
    11161 atgttgagtg atcccactcg tcttaaaatt ctgctatatc tgaaagacgg ggaacaaaat
    11221 gtgacagcaa tcacccaagc agtggaaatg gagcagtctg ctgtatcgca tcaactacgt
    11281 ttattaagag aaaatcatgt cgtcaagtct catcgcgagg gaaaagcgat tttgtatagt
    11341 cttgatgatt ctcatgtact ggatattttg aaccagacac tcaaacatgt ggaacatcaa
    11401 taacgatagg tgcaaataga taaacactgg agtgtgacaa cgaaatttct tcgttgtcac
    11461 actccagtgt tttttaagaa aaagacaaaa atgattaatc gcttttttct gttgtttcag
    11521 ttccataata actatgatct tctaggtcta aagcattcaa aataagcgaa gccgtttctt
    11581 caatagataa agatgccaca ttgatcacta cacatcctag tttttcataa agatcattcg
    11641 caaaatcgag ttcagctcta attttttcga tatcggaata agcagtatca gggtttaatc
    11701 cgtaagagcg catccgttct ttacggatat tatttaaaat atctgggtta ttcgttagtc
    11761 ccacgatttt tttaggatcg atctcccaaa gctgtttggg aatatgtgct tgtgggatca
    11821 aaggtaaatt ggcaactttt aaatttttgt tagctaaaaa caagctcaaa ggcgttttgg
    11881 aagttcggga tacgcctagc aaaacgacat ctgcttctaa aaaaccacga ggatctttcc
    11941 catcatcgta ttttacagca aattccattg ctttgatccg tttgaaatag ttctcattca
    12001 ataaatggag ggcaccaggt tgccgaattg gcttgatacc ggttaaacgt tcaatttctg
    12061 atatcggtgg cgtcatcaca tcaaaactaa ataattgatg ttcttcacaa aaagaacgtg
    12121 ttatctcgat cagttcttga ttgattaatg tatgcaggac aagtgctttt tcttctaatg
    12181 catcttgcaa tgcctgcttt aatgtttttt catctgtcac gaacgttctg cggaataggt
    12241 tgaactcaac ggtaggatac tgagccatag tagcttgcgc tagttttgtc gcagtttctc
    12301 cagcagagtc ggagatgaca aaaaaagtaa cgatttctgt agaatcggaa gacatggata
    12361 aaacctcact tgcatttttt ttatgtttat tataccatac tctagaatgc gtacttcgta
    12421 atcattttga tttggaggaa taagtcatga aaaaacagac attgatcgaa caatcattag
    12481 aaacattgaa aaataacggt tttaaatata ctaaaaaaag agaagcattg ttaacttatt
    12541 tagtgaagcg taaccgctac gtatctgcta aagaagtttt tgactatatg aatcaggaat
    12601 tcaaaggggt aagttatgat acgatttatc ggaatctcca tgattttgca gagatcgatt
    12661 tactggaaga aaccgattta aatggagaaa tgaagtttcg ttttcattgc tgtcaggggg
    12721 aaatcggcca tcaccatcat tttatctgta cagtgtgcgg aacaacgaaa gaaatccata
    12781 tgtgtccgat gaattatttc gaagaacagc taggtggatg cacgatcgaa gggcatagat
    12841 tcgagatttt cggaagatgt gaaaattgtc aataagggtt taaataaaat aacatttgat
    12901 aattttaaaa aaaagcaaga tttccttgac ggtcaaaaca gcattagata taatatttac
    12961 aggacagtct tgttttttga gatatatttc aataacaatt acggttactt tttaagctcg
    13021 gagggaggga attaacatgt caaaaacagt cgttcgcaaa aacgaatcac ttgacgatgc
    13081 tcttcgtcgc ttcaaacgtt ccgtgtcaaa agcgggtact ttacaagaat ctcgtaaacg
    13141 tgaattctac gaaaaaccaa gtgtaagacg caagaaaaaa tctgaagcag ctagaaaacg
    13201 caaaaaattc tagttctgtg atgaattgga gttggtagtg tgtcactact aagtagatta
    13261 aacgatgaca tgaaagcagc gatgaaagcg aaagataagg aatctttaca agtcatccga
    13321 atgatcaagt catctataca aaacgagcag attaaagaag gtcacgactt gacagaagaa
    13381 gaagaactaa cggtgttgtc tcgcgaaatg aaacagcgac gtgattcttt gcatgagttt
    13441 gaagaagcag gacgtgacga tttagctgaa aaagtaaaaa gtgaaattgt aatagtcgaa
    13501 aaatatatgc cggaacaact ttccgatgaa gaaattcgac aacttgttca agaagcaatc
    13561 actcaaactg gtgcttcatc aatgaaagaa tttggcaaag ttatgggtgc aattatgcct
    13621 aaagtcaaag gaaaagcaga tggaaaccaa gtgaatgcga ttgtgaaaga actattacaa
    13681 tcttaatttt atcatttaaa ctgcggacag tgattgtccg cagttttttg tgttcgtaaa
    13741 aaaagaa
//
LOCUS       DACDPS010000065        13278 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.065, whole genome shotgun sequence.
ACCESSION   DACDPS010000065 DACDPS010000000
VERSION     DACDPS010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.065"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            14..160
                     /locus_tag="IWJ07_002011"
     CDS             14..160
                     /locus_tag="IWJ07_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAP9333584.1"
                     /translation="MLVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEENRKKAMRK
                     RVSR"
     gene            complement(222..2033)
                     /locus_tag="IWJ07_002012"
     CDS             complement(222..2033)
                     /locus_tag="IWJ07_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAP9333585.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(2030..2395)
                     /locus_tag="IWJ07_002013"
     CDS             complement(2030..2395)
                     /locus_tag="IWJ07_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAP9333586.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(2558..2953)
                     /locus_tag="IWJ07_002014"
     CDS             complement(2558..2953)
                     /locus_tag="IWJ07_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP9333587.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(2971..3933)
                     /locus_tag="IWJ07_002015"
     CDS             complement(2971..3933)
                     /locus_tag="IWJ07_002015"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAP9333588.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(3933..4145)
                     /locus_tag="IWJ07_002016"
     CDS             complement(3933..4145)
                     /locus_tag="IWJ07_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAP9333589.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(4166..4864)
                     /locus_tag="IWJ07_002017"
     CDS             complement(4166..4864)
                     /locus_tag="IWJ07_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP9333590.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(4884..5426)
                     /locus_tag="IWJ07_002018"
     CDS             complement(4884..5426)
                     /locus_tag="IWJ07_002018"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAP9333591.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(5558..6562)
                     /locus_tag="IWJ07_002019"
     CDS             complement(5558..6562)
                     /locus_tag="IWJ07_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP9333592.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(6559..7548)
                     /locus_tag="IWJ07_002020"
     CDS             complement(6559..7548)
                     /locus_tag="IWJ07_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP9333593.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(7545..8351)
                     /locus_tag="IWJ07_002021"
     CDS             complement(7545..8351)
                     /locus_tag="IWJ07_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9333594.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8517..9473
                     /locus_tag="IWJ07_002022"
     CDS             8517..9473
                     /locus_tag="IWJ07_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9333595.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(9550..10068)
                     /locus_tag="IWJ07_002023"
     CDS             complement(9550..10068)
                     /locus_tag="IWJ07_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9333596.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(10156..10305)
                     /gene="rpmG"
                     /locus_tag="IWJ07_002024"
     CDS             complement(10156..10305)
                     /gene="rpmG"
                     /locus_tag="IWJ07_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP9333597.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            10533..10979
                     /locus_tag="IWJ07_002025"
     CDS             10533..10979
                     /locus_tag="IWJ07_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP9333598.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(11174..13069)
                     /gene="asnB"
                     /locus_tag="IWJ07_002026"
     CDS             complement(11174..13069)
                     /gene="asnB"
                     /locus_tag="IWJ07_002026"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAP9333599.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         4122 a         2820 c         2265 g         4071 t
ORIGIN      
        1 atttgtatct ggaatattag taggaagtct ggcaacagcc gctgccgttg ctggtttggt
       61 tgcttctgtg aaaaaaacag tgatcgatcc aattgacgaa aaagaagcaa tgatcgaaga
      121 aaaccgtaaa aaagcaatgc gtaaacgcgt ttctcgttaa taattattta aaaatacgca
      181 tgaagacttt ctaaaaaaca gataagtcat catgcgtatt tttatttgtc agtcaatata
      241 agttgtgcta ttttatctgc ctgtctcaga ttccatggat ctgtataagg tctagctact
      301 gctgcagtta gccaatccac accttcaaca ggtattccat agcaataaaa gtcagactgg
      361 atttcccctg aagcatcaat cacgcgattc gtacctcgct caacatctat agcttctgaa
      421 tgataacttc ctgtattatc tgaaaatgaa aaagggcgaa caagtttgtt ttccttcaaa
      481 ttttttatga caggattact tgaacgagaa aagcttgttt gtggcaatcg agcttcaatc
      541 aaatagcgac tgctgatttc tttatcttgt agagaaataa catagcggtt gtgcttttgc
      601 tgaatatcaa atggcggttc gatcaaatga aaaatacccg catctatcag tgctgctaat
      661 tctttcgtcc gaatgattgg cggaccgatc gttaaaaagc tatttagatg agtaaaccat
      721 ccccatagta tttctttgta ttccttagca gaaaaaacgt gccattcaat agcctgccgg
      781 attggatcac gccagtcttt taatgcatca aatgttgaag caaatgctcc tgtgcaattg
      841 cctttttccg cttcttttat ctgcccttca ataaatattc ggctagcttc tgaaaagggc
      901 tcattctgat aggcaagagg agtttctaaa tacgaccaat cccatctata agggattagt
      961 gctggatgct ttccgagaca ctcctctgaa gagagggaga gaaaatcatg ctcaaaattt
     1021 ttctttggaa tcggcaagtg atattcttcg atgattttct tgtaataaaa caactcaacg
     1081 tcttttttca gatagtcaaa aaatgtttcg ccagtcaaaa atcccgactg atgccattgc
     1141 tccaggtttt cttttgttag taaccgtggc catgccattg ctccgccttg cttttgattt
     1201 cttcctcgag ggtaataagg gagccccctt cttgatccac tgtatacaat cggttccttg
     1261 cctgatggat gatagaccag ttttcccttt tcgttctcga agcgccctcc tcttgatact
     1321 gtaaacaatc cgacataatc aaaaaaactt aagcctagac ctcttaaaaa gacattttct
     1381 ttaggcggta tcttcgaaag atctacatct gcaggattag aaggaagctg atacagtaaa
     1441 acatttttct ttgcgtagtc tacccacact ctttcttctt ctgtctcatc attttcccag
     1501 tgaccgcttg ctaaaacaag ctgatcgata gaatagcttt gctgatctgt ctgtaaaaga
     1561 taaccatctt ttcttttgct tacttttttt actaaagaat aaacaaactg aatagaagct
     1621 ggaaattctt cttgcaactt tgagaaaaac cagcgttgat agagtccgta taaacatcga
     1681 gtagattgct gatctttttc cagacgattg atttcttcta taattagttg cttatttttg
     1741 gttaccgtct gttcgatata atctgtagca ggtcctttac tccattgaaa caagttgggt
     1801 ccctgtgtta ccaatcctcc actggacaat gtttcatctg taaaaagcgt cacttgttga
     1861 gaaacagaat tcattaataa ctccggtggc tgattcaacc gccaaactcg acctccagga
     1921 ccattgggat cgaaaagaaa aagttcaaga gaatgtctgt cctgattatt atggagaatc
     1981 ctttctgcta cacttaatcc tctaggtccg gcaccaatga tccctatttt catttgcatg
     2041 cctccatttc gataaatttt ttcaataaaa cagcatggtt ctgatgttca ttttttgcac
     2101 cataaacaaa agtaatgact ggatgttctt tcataagttg actgagttta aaaatagctt
     2161 gcttcttttc ttcttcgaaa atcaattctt gctggtattt ctttgcaaat gaagcaaatc
     2221 gttctggtac atggtcaaaa gttttgcgtg cttcattact aggtgcaatt tctttcaacc
     2281 acagatctaa ctgcgctttt tcttttgaaa ttcctcgtgg ccagaatcta tccacgagta
     2341 ttctatagcc atcctctttt tcagcatcaa ggtaaacacg ttttaatttg atcatagttg
     2401 tttccttctt tctacctttt cgcttagctt atatatgtag ataatagcac aaaataaaaa
     2461 aaatgaacgg cacaactatc cattgcgccg ttcatgtgat cttggtcaaa atctaatgat
     2521 tgattcggtt tttaaagctg tcatacaatc atctgtttta tactttttta cttttgattt
     2581 ttccagacca tccgtcatat ccacccttaa gtaaataaat ctcttgataa cctgctttac
     2641 gtaatttatt tgctgtacgg atgctcaaac ttttcttttg atcgtaaata tagacaggct
     2701 ggtcttttcg aatagatcct aatgaatctt tcaaaacagt gtaagggatg tttcttgcac
     2761 ctaaaatatg cccagcatca aacacatctt tttcgcgaac atcgattact tgtgcttttc
     2821 tcattccttc tcgaaattct tcttctgtca aagtcgttgc tgaacgtttg gccattactc
     2881 gtagatagac ttcccaaagg atgattgcaa gcacaatcaa tagcaaaatg atattaatta
     2941 cccataaaac catgtaatgc ctccgttttt ttaagcaaat ttcaaggcta atgaggcagc
     3001 accaatgaca ccagcttcat taccaagctc tgctaatttg atttttgttg attctcttac
     3061 ttgcgggaaa gtaaattctt taaaatattt ctctacacgg ctacgtaaaa attctcctgc
     3121 tgccgatact cctccaccta acacgatact tgacggattt aatgtattcc ccaagtttcc
     3181 gcaagctaaa cctaagtaga agcatacgcg atcaacaacc atcaatgcaa acggatcgtc
     3241 ttcttgagcc atttcaaaga tatctttact tgttacttct tctccgttgt ctagcattgc
     3301 tttcagtttt gaatcccctg cgtattcttc tgccaagaaa cgacctaggc gtaccacacc
     3361 tgtagcgcta gatacagttt ctaaacatcc gcgtttaccg caagtacatt cgaacccttc
     3421 aggatccaca gtgatatgtc cgatttctcc tccagctccg ctcacgccgt gaatcaaatt
     3481 tccgccagca acgatacctc cgccaacacc tgttcctaga gtaacgaata caacgtctgg
     3541 atcgttttct ccagcacctt tccatcgttc acctaaagca gcaacattcg cgtcattatc
     3601 gatagcgaat ttgattcctg ttcctgcctc gatcagtttt ttcacaaatt gaacttctgt
     3661 ccagtttaag ttatacgcac caataaccgt tccagcttcg ctgtccacgc ttcctggggt
     3721 acccatgccg attccgataa agtcttcggc tttcatatta tataaattca aacgatgatt
     3781 gattgattca atgatttcag gaacgatatg cttaccatca tccaaaatat tggtatcgat
     3841 gctccatttt tgctgaatct ctccttctgg tgttaaaatt gcaaattttg ctgttgttcc
     3901 acctaaatca atcccaataa tcttcttgtc cattacaaat ttcctttctt tcggctttct
     3961 tcaattcggt gctctctttt taatatgcgc cacgctgtca tgtatgtatc atgatcaatc
     4021 aaacggctat catacagatt acgcaattcg atagtcatta attcaatgtc atagatgcgt
     4081 gcacccacat agataaagat gccaaatttt ttgaacagct gctgtacatc atataagctc
     4141 tccatcttta agcacttcct tcatattata caagtaaacc atattttttt aaacccagta
     4201 aaatacatat tacaaaaaga aatacaaaaa ctagtgtaga aagaatcctt tggtgtatcg
     4261 aatagcttgt ctgttgtttc ggtagaccca aaacattccc taacactaat ccgccaatta
     4321 gcccaccgac atgtccccaa acatctacag aggtatccag aagaccaaaa acaaatgtca
     4381 acacaataaa taaaagaaaa tgtctgacta gctgctggat cgctgggtta tgtttaaaat
     4441 gaaaccctaa gataaacaac gcaccgaaca aaccgaatat cgaagtactt gcaccaccag
     4501 acagaacatt cgcttcgtta aaagcaaagc ttgctgtatt tcccattatc ccgctgaaca
     4561 tataaatcaa gaaaaaacgc caatggccat agattgcctc gatttgctgc cccataaaat
     4621 acagtatcag tgagttcatt acgaaatgca tcagtccaaa atggatgaag attggtgtca
     4681 ctaaacgcca ccattcattg aagtgaacaa gataaggacc atacattccg gcataataag
     4741 ggatctcaag tcgcggaaat aattccatca tgaggaagac cgctgtctgt atccctaaaa
     4801 aagcataggt ccataacggt cgtcttaacc atgcacgcaa ttttatttgc tgttgataat
     4861 tcatgaaaac ctcccatttc attttagtca ataaacaatt gttggatctt ctgatcaaag
     4921 ctctccggtt gccattgttc attcaattgt tcacgaaaaa caaggctaca tgttttttta
     4981 tgaaaattat ttaaataccg gtcgtaataa ccaccgccaa aaccgattcg ataaccggct
     5041 ctattgtaga taatacctgg tacaatgagg agatcgatca tctccggcaa atagagcttc
     5101 tctgattgtg gctcaagaac accaaaactc gaacgtacaa gaacggactg ttcatccatt
     5161 tcataaaacg ccatctgacg tttcggcaaa gttttaggta caactatttt cttttgttct
     5221 ttttctgctc gttggataac cggctgtgta tccagttcaa attcattcga taaagtcgtc
     5281 cctatcactt ttgctgactt ccagtactcg ctggcgaata gctgtttata gattcgctgt
     5341 tctttttcct ttttcttgcc acttttggcc agatttttca gcttttcgat tcctttccgg
     5401 cgtaatctta ctttcaattg gtccaaaaac tcacccttcc ttctctcata aaatggtata
     5461 ggaaaaaaag aaaaatagca atcgaacttt caaataaatg cataaataga ataaaaagaa
     5521 gacctagaaa tgattggcct tcctttttta atgaatttca actgttcagc gcgtgttttt
     5581 gtctgataaa aagtgcatac agaaaatatg gcgcaccaaa aagggcaatc aaaattcctg
     5641 taggtacttc cccacttagt agaatcactc tagcaaccat atctgcacta agcagaaaaa
     5701 tggaaccgaa caaaccgctc agaacaaagg tccagttatt ttctttacgt acgactagct
     5761 tcgcagcatg aggagctagt agtcctacaa aagataaact acctgtcacc gctacactac
     5821 cgcttgccaa agcaacagct aacaacaaaa ggattttttg acttttagcc acgttaaggc
     5881 ctaatccttg cgcgcgttct tgaccaaatg atagcaatac aatcctctgt gcataaagtc
     5941 ctcctagcgg aattaagata accgcccatg gaagcaatgc ccaaacataa ggccagctcg
     6001 caccccagat tgatcccgct aaccatgcat tcacaaatcc ataattttcc ttagataatt
     6061 ggatcgtgca tagcaatgtt aatgcagaaa ggccggcatt aacggcaata ccggacaata
     6121 agattcgatt catacccaaa aagccatttc gatcataggc cgcaagatag acacagcaag
     6181 cagctaaaac accacctaga aaggctacaa ctagcaatag aaacggcagc gaatagtgcg
     6241 aaaagaaacc taaataaagc atcactaaaa atcctgctcc ggcattaatg cccaatatac
     6301 tagaatctgc caattcattt cttgttactc cttgcaagat ataaccagaa acgccaagac
     6361 aagctcctgc taataaactg atcagtaatc gaggaactcg aaattctcta acaatcagcg
     6421 ccaaagaaga tgattctttc cctatgaacg tggcaaaaag ctgatccagg taaataaaag
     6481 gcgtaccaat cattaaacta cctatagaca ggatgacaag tacaaaaagc aagaaataga
     6541 acgtccaata tatttttttc accgttgttc cctccttact tgtatcagta aaaacggaac
     6601 gccaatcaat gcaatgacta atccaaaagg tgtttcaaaa gggggattga tcatcctcgc
     6661 tgtaaaatcc gccaacataa ccaatccacc gccaactaaa aaagagacag gtagaacata
     6721 acgatagtcc gttccaacca ttccgcgaac aactggcgga accaacagcc caataaatga
     6781 aacaggtccg actaacgaaa cggcaatggc tgctaacact gcaactagac agaaaacaat
     6841 caaccgcgtt cgtttaggat gacttccaag agctaatgca tgtgtttcac tcaaatgaat
     6901 caaagatatt gcttttccta atgaaaaaga aaccaaaaca gcaagtatat ataaaggaaa
     6961 caatactgct aattgtttcc atgtgatatt ggcagctccg cctacaaacc aaaaagcaag
     7021 atcctgctgc agattaaaga attgggtaag tgcttggctg atagcagtaa aaaaagaact
     7081 aatagctacg ccagctaaaa tcagctgtaa aggattcatg cccattttcg aaaactgcaa
     7141 aaaaccaaat acgagcaaag ttacagcaaa agaacctgcc aatgacatta gaaacacaga
     7201 aaaatcagaa gctttaggcc aaaaagcaaa actaagggcc attgccaacg aagcacccgc
     7261 gttgattcct agtaagcctg aatctgctaa aggatttctt gtcactcctt gcatcaatgc
     7321 accgctcaat gcaaaacaac tccctacaag aaatgctccc gccacacgag gcaaacgaat
     7381 atttcgtatg atttggtgac tttgttttga tctatcaaaa tcaaacaacg cctgccaaaa
     7441 atctgctttt cccacagtcg ctgcgccatt tgaaagagca acaaaaaaaa taatcaaaag
     7501 aacaaaacag accaaaagca ggagtctatt tttcttcata gtcctcataa ttgatcctcc
     7561 tgctcttttg gcaacaaatc ataggttaga aaaactggtt tatttgttct tggaagcgtg
     7621 acaatcgttg catcaatctc aaatattttt ttcatacact cttttgtgat cacttcttca
     7681 ggtgtacctt ctgtgatcaa cttaccgttt ttcattgcta ataaataatc tgaaaaacgt
     7741 gaagcatgat taatgtcatg aatcgacatc aagatcgttg tttgtttcat ttgattgatt
     7801 tcagtcaata actgtaggat ctctaattgg tgtgccggat ctaaaaatgt tgtcggttca
     7861 tccaagatca agatatctgt ttcctgagca agcgccatag cgatccagac acgctgggtt
     7921 tgtccaccag acagcgttgc gatatcccgc atacgaaatc cagctagacc tgtagcatcc
     7981 aacgcccact caattgcttc atgatcttgc gtgtccatgc cactgaacag tttttgataa
     8041 gggtatcgtc cgtaagatac tacttcttct actgtcatac ctgtagggtg ttcactagtt
     8101 tgtgctaata atgcaatttt ttttgcaagt tcttttgaac tgtatgtaga gatatcagag
     8161 ttattgacaa tgatctttcc tgatttcaca ggtagtatac gggtaaaact tttcaataaa
     8221 gtagattttc cacttccatt tggcccaatc aaactagtga ttttcccttc aggaatcgta
     8281 acagacatgt tttcaatgat ttttttgttt tcgtagccgg ctgcaatgtc agctgctctt
     8341 aatctcgtca tttcacccat cctttcgctg aaaatcattc tcaattatga cgctgtttca
     8401 acatttcgtc aagcgctact ttcatatcta tagccaaatg aagcggttga cagcgttcga
     8461 aatatcagga ataataacca atgagaaaga ttctcaatta tgttaggagg aaagaaatga
     8521 aattaaaaac aaaagtattc acattatcta gcatattgct gctggctgga gtactgagcg
     8581 cttgctcccc ctcttcagat aaagaaacag ctacaactgc caattcgacg acttcatcca
     8641 atagtactca tacagtcaca gatacacttg gacataaagt agagattcca aacgcgccca
     8701 aacggatcat cggcagttac ttagaagact atctgattgc actaggggaa aaacctatcg
     8761 cccagtggac tgttggcagt ggttccattc aagattatct gcaagatgat ttaaaggatg
     8821 tcccaacgat ttcttatgac cttccttatg agaaagtgtt aagctttgag cctgatttgc
     8881 tgttgatttc ttcttccgct actgtcgaag gcgggaaata cgaacaatat agcaaaatcg
     8941 cgccaactta tgttgtaaaa aacggaacag atgtcacatg ggaagaacaa ctaaaagatg
     9001 tcggcaaagc tttggcaaaa gaaaaagagg cagaaaaaat catttccgat tatcaaaaat
     9061 ccatcaagaa aactcgagaa gaactagcag ataaaatcga gaacaagaca gctgcagtgt
     9121 tgtgggtgac caacaactca gcctttatgg tcagcaaaaa tcggtcaagt ggaagaatcg
     9181 tatacgacga tctggaattt ggtgttcctg atctagtgga agaggtctcc aaaaaagcca
     9241 cttctgactg gtctgctgtt tcctctgaaa agctagctga attagatgct gactacatca
     9301 tccttgtgaa cagtgacaaa gatgctgcca tgttcaatga accaagctgg aagaatctaa
     9361 aagcagtgac agacaatcat gtactgcaat tcggacctga atctagttgg ctatataacg
     9421 gacctattgc cagtcaggaa atggtcaatg atatcaaaga aaaactaaat tgattttaac
     9481 taaaaaaagc atcttcacga ttagtcgtga gatgcttttt ttcttattaa caaactagac
     9541 gcatagattt tattcttctt ttgaagcaac cctatttttt actctttccc atttttgaaa
     9601 acgatgggca tatagatttt cttgatccgt tatcccctca tactcctcta taagggcgaa
     9661 gtctgaaaaa tcaatattcc caatatacgt atcgccttca aacgtgctat cgatcaatgt
     9721 tcgccagatc cgaccagtgt aaggaagaag ttcttgaaaa atccgactac cacccgaaac
     9781 atagatatct tctgagatcg tttgactata cgacaaaagt tcatcgatcg aatgcattac
     9841 ttccgcattt tctttcgtgc gaaaatttgg ttgcgttgtc aacacgatga tatggcgata
     9901 aggaagtgaa agatccccca ttccttcata ggtctttctt cccatcacta aaattcgatc
     9961 cattgtgtgt tcgcgaaaga aacgcatatc atttggcaat cgccatggta acaaaccatt
    10021 tttgccgatc agaccatttt tatcttgtgc ccacatggaa ataaacatat tatccgctcc
    10081 ttatgatttt agatgttttg agataaaaaa agtctcaatt ctttgaccag caaggaattg
    10141 agaactttaa aaacattatt ttgtttcacg gtgcaaagta actttgcgtt cgcgtgggca
    10201 atatttttgt ttttccaaac gatcaggatt gttacgtttg tttttgcttg ttagatagtt
    10261 acgttcttta caagaagtac actctaaagt gatgtttacg cgcatgtttt tccctcccat
    10321 ttttacaaga atcctctaag atgactctca gacttgatta tcttaccatg attttttcta
    10381 gattgctagt gtttttcacg attttgttaa gtaaaactac ttgacgataa atacaacggt
    10441 tttacttctc aaacaaaatc tttttctttt tgtataaata cccttacact tgtttatata
    10501 gattatgttt tttaataaag ggggatacta atatgccatg gaacatgaag gactttcctg
    10561 catcaatgaa aaacttagat aaattgacaa gaaaaaaagc aatcgacatc gcaaatgcat
    10621 tattagatga agggtaccct gataaccgtg cgatcccaat tgccattgat caagcaaaaa
    10681 aatggtatga agatgctagt gaatcagaaa aacgaacttt cgaaaaagaa aaaaatccaa
    10741 gcaagacgga tgaacacgat acgaatcctc gtgctggaaa actgcttgat tcagatgtga
    10801 tcgtagaata tgaggaagaa caatggatcg tgaaatctaa aggggcaaaa aaggcaagca
    10861 accattttga tacaaaaaaa gaagcaatcg aaaaaggaaa gcaagtagca caaaacaaag
    10921 aatcaacttt gttcatttat aaaaaagatg ggacaaaaga aaaagaaatc gattattgaa
    10981 catatgtaaa ggggttgtga cgaaaatcgt gtcacaaccc ccttatgttc gaaacaatgg
    11041 tgttcctctg ccaactccga accataagtc ataatatcta taaaacggga agagctgttt
    11101 tttgaaattg tttcttatca ctcagtgtta aacggtagga gaaaacctct tttttattga
    11161 tatttgattt tcatcaaaca gtcacatagt cgattgcttc tgcttgcgga atcgtttcat
    11221 ctacaaagta gactttatac catgtcaaga aactgaagat tgtccagatt tttctttgct
    11281 cttctttacg tccttcgtga tggtcatcta gcaacttcat gattttttct tgatcaaaaa
    11341 attcttttgc aaaatctgct tcgaataatg ctttgacttg ttcgtagccg tgttcttctt
    11401 tcaaccaagc tttgattggc acagggaatc ctaatttcac acgattcgac cattcttccg
    11461 gaagatgttt gttcgcagct tgtctgaaaa tatcttttgt gttgttttga ttcaataaaa
    11521 acttacttgg tatttgttga gccaatttca tgacttcgat atccaataaa ggtactcgga
    11581 cttccaaaga acttgccata gaaagtttat ctgcctttaa caagatatct tttggcatcc
    11641 attgatgaag atcgacatat tgcatcttat tgacttcatc tttgattcct tctgttttct
    11701 cgtaatgaac agtcatgatt tcatccacag atggtgctgt ttgatatttt ggtgttaaca
    11761 cttctgatgc ttctgattct tcgaacactc gagcatgacc gataaaatat tcttttgcag
    11821 gagcaagtga ttcatataaa tggatccgtc catggaaatt tttcattttt ccgattttac
    11881 gtcctaaatt atagcgtgtt ccttttggta attttttcaa tccttgagca atcacacgga
    11941 tgaattttga attcgtatgg aatccatacg tttggtatcc cgcaaacagt tcatccgccc
    12001 cttctccaga ttggactacc cgcacacttt gtgaagcaag atttgctaaa aagtacaaag
    12061 gtacgcaaga aggatttgaa tcaggttcat ctaagtgata ctggatcaaa gggaatgctt
    12121 tgaacgacat gtctccgtcg atcactgctg ctgtattatc taagtccaat agttcggtta
    12181 gttttctggc ttcaatcgct tcattatagg ttttatcatc aaacccgatt gagaaagaat
    12241 gatctggttt caatacagag gttacataac tggaatcaac cccgctggat aagaaagaac
    12301 caacttctac atcactgacc gtatgtgctt caatagaagc ttgaacggtt tcgtcgattt
    12361 tttcgatcca ttcttgtcgg gaatgcgttt cttttgtttc aaaatctgca tcccaatatt
    12421 gctgaatgtc tatttttcca tctttatacg tataataatg gccttcaggc aaacgataga
    12481 catctttgaa gaatgtttcg ccgtttaatg gagaatattg gaaagtcata taaggtttca
    12541 acgcttcttt gttcaattct ttgttaaaat caggatgagg cagaaaactt ttgatttctg
    12601 aaccaaacat aaatgttccg ttcatttctg cataataata tggtttgatc ccaaagtggt
    12661 ctcttgcacc gaataactca ttcttttcat tatcccaaat agcaaaagca aacattccgc
    12721 ggatcttttg caataattct gttccccatt cttcgtaacc atgcaaaagg acttctgtat
    12781 ctgcatgtgt ttgaaaaacg tggccagctg caatcaattc ttctctcaaa ggctggtaat
    12841 tatagatttc gccattaaaa ataatgatct ttgtccgatc ttcattgtaa ataggctgag
    12901 taccgccctc taagtcaata atacttaatc ttctgaatcc taaagcaaca tgtttatcga
    12961 tatattctcc tgaactatta ggtccacggt gaacgattcg atccatcatt gtattaataa
    13021 ttgttttttt gttgtccttg tcgttaacaa aacctacaat accgcacatt taactttcac
    13081 atcctaatat aaaattactg tttatttcat tcatatgcat cttatcatag aataaatagc
    13141 ggacgaatat tttctctctt acaatttcgt cacgtgaaaa caaaagatga aagtttctgt
    13201 ttttacagaa aataaagtcc caatatctaa tttaccatgt tttctttttt gaggctacct
    13261 tattctcttc gtgttaaa
//
LOCUS       DACDPS010000066        13108 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.066, whole genome shotgun sequence.
ACCESSION   DACDPS010000066 DACDPS010000000
VERSION     DACDPS010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.066"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            143..1084
                     /locus_tag="IWJ07_002027"
     CDS             143..1084
                     /locus_tag="IWJ07_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAP9333600.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1153..1884
                     /locus_tag="IWJ07_002028"
     CDS             1153..1884
                     /locus_tag="IWJ07_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAP9333601.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2028..4208
                     /locus_tag="IWJ07_002029"
     CDS             2028..4208
                     /locus_tag="IWJ07_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAP9333602.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4228..4710
                     /locus_tag="IWJ07_002030"
     CDS             4228..4710
                     /locus_tag="IWJ07_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAP9333603.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            4726..5487
                     /locus_tag="IWJ07_002031"
     CDS             4726..5487
                     /locus_tag="IWJ07_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP9333604.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            5618..6952
                     /locus_tag="IWJ07_002032"
     CDS             5618..6952
                     /locus_tag="IWJ07_002032"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAP9333605.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7259..7744
                     /locus_tag="IWJ07_002033"
     CDS             7259..7744
                     /locus_tag="IWJ07_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAP9333606.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            7737..8420
                     /locus_tag="IWJ07_002034"
     CDS             7737..8420
                     /locus_tag="IWJ07_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAP9333607.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8546..11191
                     /gene="polA"
                     /locus_tag="IWJ07_002035"
     CDS             8546..11191
                     /gene="polA"
                     /locus_tag="IWJ07_002035"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAP9333608.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELADKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11236..12072
                     /gene="mutM"
                     /locus_tag="IWJ07_002036"
     CDS             11236..12072
                     /gene="mutM"
                     /locus_tag="IWJ07_002036"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAP9333609.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12036..12665
                     /locus_tag="IWJ07_002037"
     CDS             12036..12665
                     /locus_tag="IWJ07_002037"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAP9333610.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            12717..13049
                     /locus_tag="IWJ07_002038"
     CDS             12717..13049
                     /locus_tag="IWJ07_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP9333611.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHISDEQKIEEQRMKH
                     YTGIEEKL"
BASE COUNT         4424 a         2318 c         2891 g         3475 t
ORIGIN      
        1 aagcgaagtc tttttaattc aaataataat attatgtaaa caaaaaactt tccatgcgta
       61 attatacaca agcaaataaa tcgtcaaatg cgaacgcctt ctttatactg tttaggaacg
      121 aaaagaagga aggaagaaat acatggatta tcaagtactg ctttattaca attatacaaa
      181 aatcgaagaa ccagaggaat ttgccaaaaa tcatttagcc ttttgcaaat ctttggatct
      241 taaaggtcgt attctagtag ctgaagaagg aataaatggt actgtgtctg gaaccgttga
      301 aaacacgact gcctatatga aagcaatgaa agctgatcct cgatttgctg atacgatgtt
      361 caagatcgat ccaagcgaag gacatgcctt taagaagatg tttgtaagat accgtccaga
      421 gcttgtaagt ttaaagctag aagaggacat tgatccaaac caagtgacag gtgcctatct
      481 ttcaccgcaa gaatttaaag aagctatttt agatgaacag acagtggtga ttgatgcacg
      541 gaatgattat gaatatgact tgggacattt tcgaggggct gtacgcccag atatacgcag
      601 ttttcgagag cttccagact ggatccgcga gcacaaagaa gaatttatgg aaaaacgggt
      661 agtgacctac tgtactggtg gcattcgttg cgagaagttt tctggttggc ttgtccgtga
      721 aggattcaaa gatgtcggac agctgcatgg cggtattgct acttatggta aggatcctga
      781 agtccaagga gaattatggg acggcaagat gtatgtgttt gatgaacgga tcgctgtcga
      841 tatcaaccaa gtagataaac aaatcgtcgg tcgcgactgg tttgacggaa caccatgtga
      901 acgatatatc aattgtagca atcctgagtg caatcgtcag attcttgctt ccattgaaaa
      961 tgaagaaaaa catttaggag catgtagcat agagtgtgcg aaacatccgc gcaatcgcta
     1021 tgttcaagac catggaatct ctccagaaga agcagaactg cggtttacag ctttaattaa
     1081 atagaaaaca atagttttga ttgtcgacca tttccaatat gcgctatact agcgttgagg
     1141 atggtgatcc acatgaaatc attgatttat gctattgggg atgtccatgg caaacatgag
     1201 ctttttgaaa aacttattca ctattatgat ccaaagatcc atcagcttgt tttattaggg
     1261 gatctcaatg atcgaggtcc tcgtacaaaa gaatgttttt tattaggaaa gcaattagtc
     1321 gaagaaacag gagccattta cctccgggga aaccatgaag aatatttttt gcaatttctg
     1381 caatcaccgg aggactggta tgcgccttat atgagaaatg gtggaaaaga aacgtttgaa
     1441 agcctgattc acccaggggc atcggaggaa tactccccaa ctgaaatctc gatgatgatc
     1501 caatcgagat attcagaact gattaaattt ttagtccagc gccccttgta ttatgaatgg
     1561 gaaaattatc tttttgtcca tgccggtgtg gatttgacaa agaaagactg gcgtaagaca
     1621 agtctaagag actttatttg gataagggaa ccatttcatg aagggaaaaa caatacaggt
     1681 aagacaattg tatttggaca tacgattaca ccaatgctcc atggagatat gcagacaaca
     1741 gacctctgga ttcaggatca taaaatcgga atggacggtg gggctgtatt cggagggtca
     1801 ttgcatgggg ttgtttttga caaggaaggt attcttcaag atattgaata tcagaacaac
     1861 gaaggacctt ggcatggaat ctgactaatg gaaagaatga aggagctgtg actttgttgc
     1921 ggctcttttt atttttatag agaatattgt ttctttcagt agtttaaccc aggataaaaa
     1981 gtatgataga ataaagaagc aatttttagc agaaaagagg cattttaatg acggaaaatt
     2041 tgaaccagac gatcattcag cttgtacaaa aagaactttc agactatcgt cctaagcagt
     2101 tgactactgt acttaatttg ttaaatgaag gaaacactgt tccttttatc gcgcgttacc
     2161 gaaaagaaat gactggaagt cttgatgaag tccagattcg tgaaatcgaa gagcggtatg
     2221 cctatcttga aaacttagaa aaacgtaaaa ctgaagtgat tcggctaatc gatgaacaag
     2281 ggaaactgac tcccgagtta gaagcagaaa tcacccaagc cgtgaaaatg caacaagtgg
     2341 aagatttgta tcgtccatac aaacaaaaac gacggacaaa agcaacgatt gctaaagaaa
     2401 aaggacttga accactcgcg aagtggctga tgcagcttac agatggagag gtgcaatctg
     2461 aagcagaaaa atatattgac aaagaaaaag aagtctcttc tgccgaagaa gcgttgcacg
     2521 gtgctcatga gattattgct gaacaagtca gcgataatgc aaaatttcgg acttggattc
     2581 ggtcttatac gtataacaaa ggtatgtatg tcagcaatgt caaggatgag caagcagatg
     2641 aaaaaggcgt atatgaaatg tattatgact ttgcagaacc cgtccataaa atggtttctc
     2701 atcgaatatt agctactaat cgtggagaaa aagaagatat tctgaaagta ttctttcaag
     2761 tagatgaagc agcaatttta gcttacttgg atcgccaaat agtcaaaaat cctgcctctc
     2821 cttcttcttc attcgtcaga gaagcctatc aggatagtta taaacgcttt attcagccag
     2881 caatcgagcg agaactgcgc aatgaattga ctgaaaaggc agatgagcaa gcaatcgcta
     2941 ttttcggcga aaacctccgt aatcttttgt tgcaaccgcc actaaaaggt aaagttgtcc
     3001 tgggatttga tccggcttat cgtactggat gtaaactagc agtcgtagat gcgacaggga
     3061 aagttttagc aatcgaagtt atttatcctc ataaacctgc agcgcaagca aaaagagaag
     3121 ctgctggacc agcttttatc caattaatta ataaatatca agtagatatg gtagctatcg
     3181 gaaatggtac agccagtcgg gaatctgaac tttttgtggc tgagcagttg aaatccgccg
     3241 accataaaac ctattacgct atcgtcaacg aagcaggcgc ttctgtttac tccgctagcg
     3301 aaattgcacg aaaagaattt ccgcatctac aagttgaaga aagaagtgct gtttctattg
     3361 cccgtcgcct gcaagatcct ttagctgaat tagtcaaaat cgatcctaaa gctgtcggag
     3421 taggccagta ccaacatgac gtttctcaaa aacgtttggc tgaacaattg gattttgttg
     3481 tggaaacagc ggttaaccaa gtaggtgtgg atgtgaatac agcaagcccg cagctcttgc
     3541 agcatatatc aggattgaat aaaacaactg cccaaaacat tgtcatttat cgtgaagaaa
     3601 acggagaatt caccgcacga actcaattga aaaaagtgcc acgcttagga ccaaaagcat
     3661 atgaacaagc aatcggattc ttgcgagtcc ctggtgggaa aaacattttg gacaacacag
     3721 gtatccatcc cgaaagttat tctattgcaa aagaaatatt aacttcagtc caactttcag
     3781 aaaaagagct tggtacagaa gaagcagtag aaaaattgac acgattatcc gtagagaaac
     3841 tggctgaaag cctatcagtc ggagaagaaa cgttagccga tattttagct gggctgacac
     3901 aacctggacg agatatgcgt gatgagatgc cagcgccctt attacgtaca gatgtattga
     3961 gtatggaaga cttgaaacca gggatggaat tgactggaac ggtccgtaat gtcattgact
     4021 tcggcgcatt tgttgatatc ggtgtgaagc aagacggact tgtccatata tcgaaactca
     4081 gcaaaaaatt cgtcaagcat cctacagatg tcgtgtcagt aggagatatc gtaacagttt
     4141 ggatagaaca agtcgatacg aaaaaaggca gaatcagttt gacgatgctt tccccttatg
     4201 aagagtaaca atgaggagta acaaaaattg acacaagaag aactgcagca gttagtagaa
     4261 gagatatcat taaaaagctt ccagcttcct ttcaagcacc aagcaatgtt caatccacgc
     4321 ttacgtacaa ctggcgggcg ctatcattta aacgatcatc atttagattt taatgcgaag
     4381 ctatttgaag agtcagacga agagacgatt gcaggaatca tcaaacatga actttgccat
     4441 tatcatcttc atctagctgg aagagggtat cagcataaag atatggattt caagtatctt
     4501 ttgaaaaaaa caggagggtt gcgctatgca ccagccttga cgaagaaaca gataaagatg
     4561 gagtattatc gatgccaaga ttgttctgcc acgatctgcc gcaagagaag aatcgatgta
     4621 cgcaaatatc gttgtggaag atgtcatgga gaaatcaaat ggattgagac aaaaagagaa
     4681 gactttaatg aagcacaaaa aatcagatga aaggatagaa ggcctttgga aaaaagtgag
     4741 cagaggaatc taaaagactg gaatcagcta ttaaattttt tagccgcacc tgaagaggga
     4801 aattttgagg aactgacggc acctacactt attttagctg gaaactgtct tcctatctta
     4861 gctgataaag cagcacaaat gtatttaaat ggccaagtag atcaattgtt tttggtcgga
     4921 ggggtggggc atgcgactag aatcctttat gagaattttg aaaagcaagg atttcatttt
     4981 gaagatggca tgagtgaaag tgaaatctgt cgtcagtatc taaaagaagt ctatgatttg
     5041 cccgataagg cttttctgat agaatcgaag tctacaaatt ctggagagaa cgccactttt
     5101 tcactggaga ttcttcattc cttaggagct gtaccagaaa aaatcttatt gatgaatgat
     5161 ccgacactgc aacgcaggac tcgagcaact tttgagaaag tatggcaaaa tgaacaaact
     5221 attttcgcga atgctgtgcc atttgtgccg gaaatccttc acttttctga agagatcatc
     5281 tttacagcaa aagaactgaa tcatcaatgg cctaaggaat atttccatgc gctagtatta
     5341 ggagaaatgg aacgtctcca tgacgatgag aacggctatg gtccgaaagg aaaagatttt
     5401 attcctcaca ttgatatatc agaggaagtt tggtcttctt acaccagaat taaagagtca
     5461 ataaaaacgg atttttctcg tacttgacca ttttagcaag cgaatcctcc tttccaaata
     5521 aatttggttg aaattttcaa tcgtttataa gataatgagg agcgtgttga atgtaatagc
     5581 taaaagaatg gacgctagat aagagaagag gattcaaatg gaaaatcaaa ataaattgta
     5641 tcattttgtt ggtatcaaag gttcaggaat gagctccttg gctcttgttt tacatgaaca
     5701 aggtctaaat gtccaaggat cagacattga aaaatatttc tttacacaaa gagatttaga
     5761 aaaagcaaat atcacgattc tgccatttaa cgcagataat gtaaaaccag ggatgacgat
     5821 cattgcagga aatgcatttc ctgattcaca tgaagaaatc cagcgagcaa aagaattagg
     5881 tttggaagtg attcgctatc acgactttat tggtcatttt atccaaaatt atacgagtat
     5941 cgctgtaaca ggctctcacg gaaaaacaag tacgactgga ctgctctctc atgtattgag
     6001 tggtgtccgt ccaacaagtt atctaattgg agatggaaca ggacatggtg atccgcaagc
     6061 ggaattcttt tcatttgagg cctgtgaata tcgccgtcat ttccttgcct attcaccaga
     6121 ctatgcaatc atgaccaata tcgattttga tcatccggat tattatacaa gtatcgacga
     6181 tgtatttaca gctttccaaa caatggctgg acaagtgaaa aaagctattt ttgcgtatgg
     6241 tgacgatgct tatttacgca aattaaaagc aaatgtgccg atttattatt atggtgtcac
     6301 agaaaacgat gatatccaag cacgaaacat cgaacgcaca acttctggtt ctgcttttga
     6361 tgtctatcat ggggatgaat ttgttgggca cttcactgta ccagcattcg gtaaacacaa
     6421 tatcttaaat gctttaggtg ttattgcagt agcttatttc gaaaaactgg atctgaaaga
     6481 agttgcagaa gaaatgctga ctttcccagg agtaaaacgt cgattcagtg aaaagatcgt
     6541 agcagacatg accgtagtcg atgattatgc gcatcaccct gcagagatca aagcaacgat
     6601 tgatggcgca cgccaaaaat atccagataa agaaataatt gcagttttcc aaccccatac
     6661 gtttacgcgt acgattgcat tgatggatga atttgcagaa gcacttgatt tagctgataa
     6721 agtttactta tgcgatattt ttggttcggc tcgggaagaa caaggaaacg ttaagattga
     6781 agaccttgga gcgaaaatca aaaaaggtgg agaagtcatc aaagaaaata atgtatctcc
     6841 attgttagat tatcatgatg cagtagtaat cttcatgggt gctggagatg ttcaaaaatt
     6901 tgaacaagcg tatgaaaaac ttttaagcag cacgacgaaa aatgtactgt aatacaatta
     6961 ctgcagtgtc cttataaaaa tagaaagagg gggagtggca aaaatcttgc caagccccct
     7021 tttttgcaac ttatgataaa cggtgttcaa aagcttgcat ctgcggtaat aagcccaatc
     7081 aaatcaaaaa catgagaagc tgttttttct tggctgttct tattatttga tgcaggacag
     7141 ctttttcacg acctctgtaa actataggaa tctgttaaat caagggagca gaacttgcct
     7201 tttacgctac ttttggtaca ataatgtatg attaaaaata aactggaggg tttctatctt
     7261 gaacataggt aagccgagaa aactaggaaa aacaataaat gaaatcgagg aaggcgactc
     7321 gttatcctta acagaatcta ttgaagataa ccagcttttg ctgtatttgg gattgacgaa
     7381 tgatgcaaat cctttgtata tccaacatga ctttgcacaa aaaacagaat atcaacagcc
     7441 aatcgttcca tcagtgatgc tgatggggat catcacaagt gcaatttcaa agcatcttcc
     7501 tggaccagga tcacatgttg tcaatttttc tgtcaatttt attgaaccgg ttttccatta
     7561 tgaaacactg acttttcagt ttgaggtcat aaaagtagat aagatgaagg atgtggtgac
     7621 gatttctgta gaagcggaga atacgaacga agatcgtgtg ttggatgctg tcgttatggt
     7681 ccaaccgcca caagttcctg aattggaaat agcaatcgaa agtgaaggtg aattgaatga
     7741 ataatccaag tacatcaatg tcaggtatca atcgttttta tgcaaaagtt tatggatttt
     7801 taggtatggg acttgccctt agtgcagtca cttcttactt ggctctttgg gtatttcctt
     7861 tgcaattagc aacttttatc aataattttc cactaggctt tgtgggtctg tggatcgttg
     7921 aactgatttt agtaatggtt ctaggcgtaa aagcacaaaa gaatccgagc ttagcaatcg
     7981 gtggatttat tgcctactct cttttgaatg gcataacatt agcggttact cttgcatact
     8041 acgatattgg aacagtaacg caagctttct tgacagctac tttgatgttc atggggatgt
     8101 ctgttgtcgg agtaacaaca aaaagagatc ttagcggggt tggaagagca ggacaggcag
     8161 ctttaatcgg agtgatcatt gcactagtct tgaacatctt cattttgaga agtagcgtag
     8221 ttgagctgtt catctcccta ctgttagtcg tcatctttgc agggattaca gcttatgata
     8281 accaaagcat ccgtaatatc tatgtccaga caaacggaac agcacatagt ggaattgctg
     8341 tattcatggc attgaagctg tatttggact ttatcaactt gttcttggcg tttctgagaa
     8401 tctttggacg gaacaactaa aaaagaaaaa aattcatcag ttggggctat accgaattat
     8461 ttggtatggt cccgcttttt cttttcgcat tccgcaggat tctttgttag aatgaataag
     8521 aactttacag taaggagtta agaagatgac aaaaaataaa ttattactcg ttgatgggaa
     8581 cagtgtggct tttcgcgcat tctttgcgct tcacaattct cttgaacgtt tcaaaaatag
     8641 aaacggtctt catacaaatg caatctatgc attcaatacg atgtttgaaa atgttatgca
     8701 aaaagagcaa ccgactcatg ttctggttgc gttcgatgca ggaaaaacga cttttcggac
     8761 agaaatgtat gcggagtaca aaggtggaag atccaaaaca cctggcgaat tcaaagaaca
     8821 aatgccatat atacgtgatt tgttgacagg actaggggta cagtattacg aattgcccaa
     8881 ttatgaagca gatgatatta tcggtacttt ggcagaaaaa gtagacaaag atcaatttga
     8941 tgtcgtcgtg ctttcaggtg atcgggattt gactcaatta gctagtaaag aagtaaaagt
     9001 ggatatcact gtaaaaggtg tcagcgatat cgaaagctat acaccagaac atgtagcgga
     9061 aaaatatgat gggttgacgc caaaacagat tattgacatg aagggactag ccggagatgc
     9121 ctctgacaat atccccggtg tgactaaaat cggtgaaaaa acagctatca agctattgaa
     9181 acaatatggc tcagtcgaag gaatttatga acatatcgat gaaatgaagc aaagcaaaat
     9241 gaaagaaaat ctgataaatg acaaagagca ggctttctta tcgaaaaaat tggcgaccat
     9301 caatacttca gcgcctgtgg atgtctctat cgactcattg aagtatgaag ggaaaaatct
     9361 cgataaactc gttccgtttt ataaagaaat ggatttcaat cagttccttt ctaaattgaa
     9421 tatcgtagaa gaagaagtaa aaatggatga tattttgttt gaagtcgttc atgaattcaa
     9481 agaggaaatg tttactacgg atatggcgtt gtacgtggaa atgatgggcg acaattatca
     9541 tacagaggaa atcgtcggag tagcgtgggg gacagaaaag aaaatttatg tcacgaacga
     9601 tctatctgta ttcgaaaacc gagcgttcca ttcttggatc actgatccga ctcggctgaa
     9661 aaaagtatat gatgcgaaac gaacttatgt tgctttaaac cgctatactg gaaaaacgaa
     9721 aggaatcgat tttgatgtct tgctaggtgc ataccttctc gatacgaatg aaaaaagcac
     9781 agatatcgaa ggaatagctg ctcattacgg atacaacgat atccaatcag atgaagctgt
     9841 atacggcaaa ggggcgaaaa aaggtttacc agaagaagag gaactctttt ttgctcattt
     9901 agcgcgaaaa gtagctgcaa tcaatgcgtt gacaccaaaa ttgagccaag aactggcaga
     9961 taaaaaccaa gaagatcttt tccggaaaat ggaactgcca ttgtctatca ttttggcaga
    10021 gatggaaata agcgggatca aagtagatgc gactcgctta caagaaatga agggcgagtt
    10081 ctctgctcgc ttaagagaga tcgaacaaaa aatctacgaa gaagccggcg aagaattcaa
    10141 tttgaattca ccaaagcaat taggagtgat cttatttgaa aaaatgggat tacccgttat
    10201 caaaaaaaca aaaactggat attccacagc cgtagatgta ctggaacagc ttcgtgaaca
    10261 agcaccaatc gtagaggata tcctcaccta tcgtcaaatt gcaaaaattc aatctaccta
    10321 tgtcgaggga ctgctgaaag tgatccaatc agatggaaaa gtgcataccc gttatgtcca
    10381 gacattgacc caaacaggac gattaagctc tgttgatcct aatttgcaga acatcccgat
    10441 tcgattagat gaagggagaa agatacgtca ggcatttgtt cctagagaaa aagactggct
    10501 gatttattct tctgactatt cacaaatcga gttacgcgta cttgcgcata tctcaaatga
    10561 tgaacatttg aaagctgcct tcctagaagg tcaagatatc catgccagca cagcgatgcg
    10621 tgtatttgga atcgaaaagg cagaggatgt cacaccaaat atgcgtaggc aggcaaaagc
    10681 agtgaatttt ggtatcgtgt atggcatctc tgattacgga ctttcacaaa atctaggaat
    10741 cagccgaaaa gcagcacagc aatacatcga tacgtacttt gaaaaatacc caggtgtcaa
    10801 agaatatatg gaaagaatcg tacgcgaagc aaaagaccaa ggctacgtcg aaacgctgta
    10861 tcaccgccgt cgatatctgc cagacatcaa ttcaagaaat tacaatatcc gttcttttgc
    10921 ggaacgaaca gctatcaata caccaatcca aggaagtgct gctgatattt tgaaaatcgc
    10981 gatgatcgaa ttggataaac gattaaaaga aacaggtctt caagctacga tgctgttaca
    11041 agttcatgat gaacttgtct tcgaggtacc ggaaaaagaa cttgaatcat tagataaatt
    11101 ggtgaaggaa gttatggaac aagctgtttc tcttcatgta ccactgatca ctgacagcag
    11161 ttggggaaaa acgtggtacg aagcaaaata aacattgttt aagacagagc aatataatga
    11221 aggtgaaaag aaaaaatgcc agaattacca gaagtagaaa ccgttcgtaa aggattggaa
    11281 cggttagtag taggaaaaac gattcaaaaa gttcaggtct tgtggcccaa gatcatcgag
    11341 caaccagaga cacccatctt tgaggcttct cttgttggcg aaaccatcca atccattgga
    11401 cgtagaggga agtttttaat ttttcatctc gatcattgtg aactgatctc acacttgcga
    11461 atggaaggaa aatatcaatt tacgaaagaa aatacaccaa tcgataaaca tacgcatgta
    11521 cttttctttt ttgaggatgg aagccaactt cgctacaacg atgtacgaaa atttggtcgt
    11581 atgacaatcg tcgaaaaagg agcaagtgca acttaccgag gaatcatgaa attgggacct
    11641 gagcctttgc cagatagctt cttattagca gatttcgcaa atggactgaa aaagtcccac
    11701 aaagcaatca agccattgct gctagaccaa cgtctagtga ctggtctagg taatatttat
    11761 gtagatgaag cattatggga agcgaagatc catccagaac aaccggcgaa cacactgcgc
    11821 cccaaagagg tagaacaatt acgtctatcg atcattgatg ttttggatcg tgcaatagaa
    11881 gccggaggta ccacgattcg cagttatttg aatgcattag gagaatcagg cggatttcaa
    11941 gtagcactgc atgtctatca gcaaactggc aaaccatgta tacgttgtgg aacgccgatc
    12001 gtgaaaacaa aagtggcaca aagggggacg cactattgtc cgaattgtca aaggctaaaa
    12061 atcgcaaagt aggatttgtt ttaggtttga cgggtggaat agctaccggt aaaagtacgg
    12121 cagccaaagt ctttcagtct catggatttc ctttgatcga tggcgatgtg attgccagag
    12181 aaactgttga gcctggaacg cttgctctga ggaaaattgt atcagtgttt ggccaagagg
    12241 tattgcagcc tgatgggcaa ctagatcgag ggaaacttgg gatgattgtt tttccatcaa
    12301 aggaaaaacg gcaaaaactt gatcagctat tagatccgtt tatccgaaaa gcgataaaag
    12361 atcaaatcgc ctctctaagt tccaatcatc ctttagtaat tgtagatatc ccattgctct
    12421 acgaaggtca ttatgaccac tatatggatg cagtagctgt cgtttatact acgccagaaa
    12481 ctcagcttca gcgtttgatg aaacgtaatc aactgacgct ggaacaagcg cagcagagaa
    12541 ttagtagtca acttccaatc gaagaaaaaa agcagcgtgc ggatattttg ttcgacaaca
    12601 atggaacaaa agaaaaactt gtgggacaaa tcgaaaattg gctttttgct aatcgctttc
    12661 tttgagtatc tgtgtcaatt ttgctatcct gagtatatag aaagtgaggg atagcgatgg
    12721 gatcattaaa aaatcaaaag gataaagtcg taggtaacgc aaaagaaaaa atcggcgaga
    12781 tgctcaataa agaaaatcta atcgatgaag gtcgtacgca atcagaaaca gcagaagaaa
    12841 aagaaaagca gtaccaagca agaatcgatg aaatagaaga ccagcaggca gaagaagaag
    12901 cgaagcggca agatttgact caagaacgaa aaaatccgac acctgatcgt agtaaaacac
    12961 cgctcccaga aagtgaccac atttccgatg agcaaaaaat cgaggaacag cgaatgaaac
    13021 actatacagg aatcgaagag aagttataaa atagtaaaaa atcgagatgt gacttgaagc
    13081 agatgagaag gtgttcatct gcttcaag
//
LOCUS       DACDPS010000067        12689 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.067, whole genome shotgun sequence.
ACCESSION   DACDPS010000067 DACDPS010000000
VERSION     DACDPS010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.067"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..219)
                     /locus_tag="IWJ07_002039"
     CDS             complement(<1..219)
                     /locus_tag="IWJ07_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9333612.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKF"
     gene            complement(698..1417)
                     /locus_tag="IWJ07_002040"
     CDS             complement(698..1417)
                     /locus_tag="IWJ07_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP9333613.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(1466..3112)
                     /locus_tag="IWJ07_002041"
     CDS             complement(1466..3112)
                     /locus_tag="IWJ07_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9333614.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            3291..3881
                     /locus_tag="IWJ07_002042"
     CDS             3291..3881
                     /locus_tag="IWJ07_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9333615.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            3973..5478
                     /locus_tag="IWJ07_002043"
     CDS             3973..5478
                     /locus_tag="IWJ07_002043"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAP9333616.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(5562..6914)
                     /gene="celB"
                     /locus_tag="IWJ07_002044"
     CDS             complement(5562..6914)
                     /gene="celB"
                     /locus_tag="IWJ07_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP9333617.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6914..7432)
                     /locus_tag="IWJ07_002045"
     CDS             complement(6914..7432)
                     /locus_tag="IWJ07_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333618.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(7448..7774)
                     /locus_tag="IWJ07_002046"
     CDS             complement(7448..7774)
                     /locus_tag="IWJ07_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAP9333619.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7787..9766)
                     /locus_tag="IWJ07_002047"
     CDS             complement(7787..9766)
                     /locus_tag="IWJ07_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9333620.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9787..10101)
                     /locus_tag="IWJ07_002048"
     CDS             complement(9787..10101)
                     /locus_tag="IWJ07_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAP9333621.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            10269..10562
                     /locus_tag="IWJ07_002049"
     CDS             10269..10562
                     /locus_tag="IWJ07_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAP9333622.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10640..11752)
                     /locus_tag="IWJ07_002050"
     CDS             complement(10640..11752)
                     /locus_tag="IWJ07_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9333623.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            11953..12129
                     /locus_tag="IWJ07_002051"
     CDS             11953..12129
                     /locus_tag="IWJ07_002051"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333624.1"
                     /translation="MITEVSYETLQKKKTLIVAAVLFPAPCVCRNCGSAVVDGNRKLM
                     VVKNKKKETIVHFE"
     gene            12139..12318
                     /locus_tag="IWJ07_002052"
     CDS             12139..12318
                     /locus_tag="IWJ07_002052"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333625.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            12512..12682
                     /locus_tag="IWJ07_002053"
     CDS             12512..12682
                     /locus_tag="IWJ07_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9333626.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
BASE COUNT         4043 a         2559 c         2171 g         3916 t
ORIGIN      
        1 aaatttgaaa ttttcccaac ccacccaagg actttccctc aagctagcca agaacccatc
       61 tgctgaaata tgaaaatttt tgaatgctac gacattcgcc attacaggta tgtaaaagaa
      121 aaaaatcatc caaagcatac ccggtacagc catcaggaca agtgctttat aacgccatag
      181 attcataaaa aaatgattga acccctttcg ttttttcatc taaaaacccc tttcgattca
      241 ttcgcaacgt aattatagcg ctttcaataa tgagaataaa taaacaaagt atagggaaaa
      301 tgaaacaaag tataggagag gttgtgaaat cagcgttttg gcctgagtat tagagaaaaa
      361 aacgggaaaa tagttcctca tattttgccg ttttttgagc taataccgag ggtcagctga
      421 ttgaacaccg tttatcagag gttgtgaatt cagcgttttg gcctgagtat tagggagatc
      481 gtgtccttac tatctaactc tgagtcacaa ggaacaatgg ggactgtaac aaaagtcttg
      541 tcacagtccc ttattccgaa caaacggtgt tccagaagta gcttctacgg aaataagccg
      601 aaatttcaca aaaaagccat ccgaaagtca gacggacaag tctgcttttc agatggcgct
      661 acaactactc tgcttattct tttagctatc cattcaatta tttttctctc acttgttcta
      721 gctcttcttt ggtcatgggt cgatattgcc ctttattcaa atctttaggc agccaaaatt
      781 ttcccatacg gattcttttt aaatataaga cttcttttcc tactgctttg accattcgct
      841 taacttggtg aaactttcct tcatgcaaaa tcagacggat aattgaagtt tctgtctctt
      901 gatctacttg atcaatttgg agttttgccg gcaatgtctg ctcatcgcca tcaataatca
      961 gtccttctgc aaataaacgg atatcttctt ctgtcattat accgctaacc tcagctagat
     1021 attctttctc cacatgtttt ttaggtgata gtaactgatg ggcaaacacc ccgtcattcg
     1081 ttaaaatcaa gaggccctct gtgtctttat ctaatcggcc gactggaaaa agatcttccc
     1141 gataatcctg gtcttccaat aaatcgatta cggtttgatc atgattatcc tgagtagcag
     1201 aaatgactcc tgcaggttta tgcaaaatgt agtaaaaatc tttttgatac gtaatgattt
     1261 ctcctaaata agtaaccgca tcttcgaatt catttacttg cgctttatcg cttttcacca
     1321 cttgtccatt gacttcgatc gttccttttt tgattaatcc tttgacttct ttgcggctgc
     1381 caagtcccat atctgctaaa aacttatcta aacgcatgcg tttcccccat ttcaaaagag
     1441 catcaatcta gctcttctga ttcacttatt ttatttttaa tttatgtcgt aaacgcgcta
     1501 ttcctggtcc tagtagtctt tctgccaagc ggattttcaa agctaaataa ccatacacta
     1561 aagcccctat acctgctact acaagaatca acaatagaga ctggaatttg ctatcttcgt
     1621 ttaaaaaagc accaaaaatc atctttgtga tccacgaagc caaaagcata atcaaggtaa
     1681 gaataaagat cagcaatgtc ctgcgccaga caaaacttgg tttaaagcgg gctacttcat
     1741 ggattctctt taagatcaag gtacaagaca cagcaaatcc gatcattgtc gccaataaag
     1801 ggccgtatac ttcaaataaa cgaatggctg ggtattgaag aaggagtttc actagaaacc
     1861 caataaccaa ataattaatt gccgccttgt tttcaaacat tccttgcagc atgttggaaa
     1921 ctagcatata tagtcctagg aagagtccta caaaacttgc ttggatcagt acattacttc
     1981 ctagagaatc cggtgtataa aataatgtat ttagcggata ggcaagtaac ataacaccaa
     2041 atgtcgccgg aaacataaaa aatgaaaaca actgtaaatt tccgcttgtc aattttgcca
     2101 gacctactcg atctttcaac gtaacagctt ctgtaatcaa cggtaaccca gtagacgcga
     2161 tcgatgttgc caatgcaata actaccatcg tcaacttatc tggattggca ctaaaaatcg
     2221 aaaaaagatc cagtaactgt gcattggaat actcggtgga tgcagacatg atcttcataa
     2281 aagtgaactg atcgactaat ttgaagatcg ttacccctga acccacaata ataaacggaa
     2341 tcgcttcttt aacggtatct aacaacaatt ccttcgttgc aatatttatt ttgttttcgc
     2401 tgtagtgaat aaacgccgat gtatacgctt gatgtttctt caaaaaatac aaaaggacaa
     2461 gaatacttgc tagcataccg ataaatgccg cgaaagttga ttgcgtgacc gccgtcacat
     2521 agtcaccatc catgactttc atgatgatga atgtagccaa tagcatataa aaaatacgtg
     2581 ccacctgttc tacaatctgc gacagcgcat aaggcatcat ttcttgattg ccttgcaaat
     2641 accctcgtat cacactcata caaggaaaaa ccaatacagc gatactcaaa gaacgcatcg
     2701 ttggaatcag ttcttctccg ccaccagaag catgagctag ccaaggagag gcgatgtaca
     2761 tgaacaaagc aaacaacagg cctaacccgc ccatcatctg taatgcacgg ataaataatc
     2821 gtcgtgaagt gccatattca ttcaatgaat tatagcgtgc agtttgtttt gcaatcgcag
     2881 caggtattcc tgctgtagag atcagtagaa acaatgcata tatattgtat cccatattga
     2941 ataatccatt ggcagcctta gcattttcgc ccatccaagc ataccaggga atgatataga
     3001 cagcgcctag caatcttgaa atgatattac tggcggtcat ccaagcagag cctcgtgcca
     3061 ttttttcttg cggtgttaat tgatctaatg ctggttttct ttgattatcc atcggtcaga
     3121 ttccaacttt ctacaaaaca ttactatcat tttaattggt aaaacagaaa ctggcaatcg
     3181 attttatata acttttataa gaacaattgt ttttttgaat tcggtttcgt ttttttggta
     3241 aagtaaagaa atatacagcc ttttatggct aacatttata ggagatatca atgacacaag
     3301 aaataaaaaa actacccgtt atatttttag gtcttttctt tatcacagtt ggattgaatt
     3361 ggttcttgct ccctcacgat atagcttccg ccggagtggg cgccatcgga catttagttg
     3421 aaacggcctt ttctatccaa cgctcagtta ctgtttggag catcaatctt atgatgctgc
     3481 ttctagccgc tgtcctatta ggaaaatcag tattttttaa gactgtagcg ggaagtctgc
     3541 tttttcctgt tgttttagag attgttccaa aagtggaaat gatctcctca catttctttt
     3601 cactgatttc aggaagtctt ttgttcagtc ttggggttta cacattgtac acagtcgggg
     3661 cctcaaatgg cggtataacg ataccgccga ttattttcca caaatttttt cggcttccaa
     3721 tgccgcaagg acttttactt accaatagtc ttatcgtctt tttgaattat tatgtttttg
     3781 gattgttgga gacactattc gttctcctat ctattacagt cagttcattg tttatgaaaa
     3841 tactcatccg cttgtcccct gtgtcaagaa aatttgaata aagctggatt ttgtcatagc
     3901 ggaaacaaac gatatattaa aaagagtgtg ctataataaa ccaagaaatt gaataaagcg
     3961 agggatcaat caatgagctt ctctttagaa gacatccgtc atttactatt aaaagaaaat
     4021 ctattaaaag aatttgtttc aacaagaggc tggcatcttg atgtaccaga aaaagctgaa
     4081 tttcttcagt taagttatga ttcaagaaaa gctgatgctt ctactttgtt tttctgtaaa
     4141 ggaatgaatt tcaaagaaga atatttggat tcagcaatcg aacagggtat tcaatattat
     4201 gtatcagaac aaccatacga aaattcagca gtaggaatca tcgtaacgga tatccgtaaa
     4261 gcaatggcgc ttcttgctat ggctttttac gactatccgc aaaataaatt gaaagtgatc
     4321 ggattcactg gaacaaaagg taaaacaact gctgcgtact ttacaaaagc aatcttggat
     4381 cacacaacaa ataaaaaaac agcattgctt tctactatga atacaacgtt agatggcaaa
     4441 acatatttca aatcccattt gacaacgcct gagtcattag atttatatcg tatgatgtca
     4501 gaagcagttg aaaatggtat gactcatcta gtgatggaag tttcttcaca agcttacaaa
     4561 acacaacgcg tatacggctt gactttagat gtcggaatct tcttgaatat ctcgcctgac
     4621 catatcagtc cgatcgaaca tccgacattt gatgactatt tctattgcaa gcgacaattg
     4681 atcttgaatt caaaaacagt tgttttaaat catgaaagtg actattttga tttacttagg
     4741 gaaacagccg atctattcca gattccaacg atcacttatg gtcgttcgga agaaagcaat
     4801 tatcaagtga tccgttcaga taaaggtact cacggtttta ctttatcttc tcatgaagat
     4861 cgcttagcta tctgcgatac tgcttatgat attttgctgg ctggaggatt caaccaagaa
     4921 aatgcagcca gtgcgatcat tgccgcagca ctaacaggtg cctccagtca agatgcacaa
     4981 gacggattaa aagaagctcg tgtaccaggc cgaatggatc aattgatcca atccaatgga
     5041 gcgcatgtct atgtggacta tgcacataat tatctaagct tgaaaacatt gctgaaattt
     5101 gccaaaaatg aacatccaga tggccgtgta attgttgtat taggcagccc aggaaacaaa
     5161 gcaatctccc gtcgacatga ttttggaaaa gtgctatcag aaacagctga catcgccttt
     5221 ttgacggcag atgacccagc atttgaagat ccgaaaaaaa tcgcggaaga aatcaatgaa
     5281 gcaattacta atccagattt gatcgttcat tatgaaatgg atcgtcctga agcgattcga
     5341 cgcgctttag cagaaagcgc tccgcaagat tcagtcgtta ttgcgggaaa aggcgtggat
     5401 ccctatcaaa aaatcaatgg tgtagatgaa ccttatgaag gggattatgc aatcgtgaag
     5461 cggttgattg aagaataatg gtatttatat actagtggtt tcccatttct aagaaaaaaa
     5521 gggactgtga ttttttgtca cagttccttt tttcttagaa atcattttaa cttttcacta
     5581 gctaattctg ctgccttttc tctttcttca tttaataatt tattatcata gaatttgata
     5641 aatgggaacc agataatgaa tgcgatgatt gcacaaatga ctgctaacac agcagctttc
     5701 caatcaccac cactaccaac aaatgctccg atcccgactg gtgaaggcca cggcatctgt
     5761 gcaagcatcg gtttgacgat ttccagccga atggcaaaat aagcgagtgt agcagaagcc
     5821 atcggagcta agaaaaacgg aaccgctaaa tagggattgt agacgattgg catcccgaaa
     5881 atgattggtt cattgatatt gaataatcct ggtacaagtg aggcttttcc taacactttc
     5941 agctgatcag atttcgcgag gaatgcaata aagatgatca atcctaaagt cgcacctgat
     6001 ccaccaacgg ttacataaga attgttgaat tctcctgcca acggataatt tgcgccttgt
     6061 gcgttagctg ccatattagc taatacgatc ggggtcaaga aagaagtgat gatattcgct
     6121 ccatggatcc ctacgatcca caatgcatgg atcaagaaat aaatcaccat gacaccaagc
     6181 catgtattcg tcaactgcgt aacaaaccca aatgggatag caatcatttt gaagatatcc
     6241 gttcctagtg caactaataa tccattgatg atcatcacca caaacgcaat gacaaatgtt
     6301 gggatcagag cagtgaaaga acgagaaact cctggcggaa caacatctgg catttttatg
     6361 acccaatttc tcttgacaca catacaatac aattcgaccg caataatcga cataataata
     6421 gctgtaaaga ttcctgaagt tcctaaacgt tcgacaaaag agcccatcct aataccattt
     6481 actacagttt ctgtctcatt gatagaagca atcaagctga ttttaccatc agtcatgatc
     6541 aattcaggga tacacataaa gaatgcaaaa acagatagta atgcccccgt gagcggatta
     6601 acatttaatt tttgttcatc tcgttcaatt gacgttaatt cataagcaaa cacaatggca
     6661 aagtaaatag ataaaatccc catcgtcatt gtattgacga tcatgtacaa gtcactaact
     6721 ttgaaaaaag ttgcattaaa aaatccttct aaaaatggga acggtaatgg caaaatattt
     6781 aaaaccaaaa acatcgagcc tacaatcgtg aaaggaatag aagccatccc cgctgccatg
     6841 acagcccgaa cgattttaag ttgcgcaatt ttccccatag gtcccatcag gtatttctgc
     6901 aaaaaatcaa acattagtta ttctcctttc ctaaatataa cgctttttca tattgtttaa
     6961 tacggatata ctgttggtct tggttgagtg aaattttctt taaacgatac tgtataaatg
     7021 cactggcaaa aataccgctt acaacaagaa tgaaaacaaa taactgtcct ccattgttat
     7081 ttgccataaa aggatacaag aatgtaaatc ctgaggtaaa caacactgga atcactaata
     7141 aattaaagac actctggaac aaaaaatacc actttgtcca tggaacgttc gtttggtgtt
     7201 ttcgataaag ctttacttgt tctcctactg caggcaaaat agctaaaatc agaatcatcg
     7261 gtaaactact tcctaaactt ttagctgata gaagcagaat catccattgc atgttcatga
     7321 aaaagaaaaa ggcagtcagg taacgtacga ttagataacg attgaaatac atgtttttca
     7381 aactaagttg ctttttttct aattcgattt tcctttttac tgtatcctgc acaatgatcc
     7441 ttcctttcta tttatttttt tgatacaaat aattcatttc ctgtgcaact tccaataatg
     7501 tcatcgtcgt catcagatga tcttgtgcat ggaccatgat aatttccatt tctatttttg
     7561 tcccgctggc atattctttc agaagttgag tttgtgactg atgcgcatga agaatctctt
     7621 catttgcttg tcgcaacttc tcttctactt cttcaaacat ctctttacgc atcgagcgaa
     7681 atgcttcatg tatcagtgtc cgtgcatttc cgctatggaa aatgatatca aaagaagtca
     7741 cttgtagtgt ttcactgttt attttttcag ccatctgttt tcctccttat tcgatcagtt
     7801 tgataaattt actgctgaaa ttctcaaaaa taggttcagc taagatttcc tgctgtactt
     7861 ttagctggtc aactaaccta acgattgctt tagtcacgac cgtaattcct tcattttcga
     7921 cataagaagg cgaaactaaa aaaacaaaat gaatttcttg atattgatcc cactgcatcc
     7981 cagaaggaat caatgcaacg gctacctttg tttcgattcc aactggaaca gcgggatgtg
     8041 ggactgccac tgtatcactg aatatgattt gtcccatatt ttcacgttgc tggatctgct
     8101 ttcgcatttc tcttatatat ccttcttctt catttatgct cagcttttgc agcaaatcat
     8161 gaagaatttc ttcctttttc ggttcgtacg tataagtctt gaaaaatttt tcagacaact
     8221 gttcggtgta aatctgcttt ttttgctgta gagaaaatgc tggtatcggt ctatctgagg
     8281 aatgataggg tatagattct ccaactacct ttcgtatttt acgaacatca tcttcattca
     8341 aaaaaatact gacatgtaaa actggaattt tgaaaaacat tgtcgaaaga tcaatagaag
     8401 aaataatcag gtcgattcct tttaaagttg tttcattgat ctcataatag ccttttacac
     8461 ttaccactgt aatattttta ccgaattcac tgactacgcg attctttaat agttgggcac
     8521 tgccgtaacc cgttgcacag ataatcaatg catgtagttt atgcgcagct ctttcttttt
     8581 ccaatgccgc catgagatgc aaagccaaat aggcccattc atcttcattg atggtatagt
     8641 ttttcaaagc cggcatgttg ctgaaatgcc tttttactgc gtcaaagact ttgggatttt
     8701 ctttctttat ctcctcagtc aatgggttct caatgcgaat acctcggctc aatcgaatca
     8761 tcatcggtga caaatgatcg agtagtccat tgaaaagctg gagatcctct actaatgaaa
     8821 aatgaaattc ctgagacaat cttttcagaa ctttcgctaa ttcctttgat agttcctgat
     8881 cattttgatt ttctttgtga ttcgacttag ccatgagatg taatgtcaaa taagcaattt
     8941 cttcttcggg aaacgataca cctgcgatta cctcaatacg ttgaacgatc cgctgcgcca
     9001 cttgatactc tactcgctta gaaacttctt gttcgatgcc taattccttg atttcaaacc
     9061 cttctcggag ccgtttgatc cccaaagcca agtgtaaagt cagattctgt atgataaaat
     9121 ccgaaacttt taacttagct tctcttgttt catcgagaat gataatgact agctcttcaa
     9181 aactgatgtc tgggaaaaaa cggctttgcc ctaaatattc tttcaatgaa tttccataat
     9241 ggtttccaaa aaaagtatcc atgataaaat gccgtcgatt tctttcttcc ccttcgatcc
     9301 atgtcccttg accatgctta gaaacgattt ttagtgaata tggaatcaat cgctccttga
     9361 tttcttgcat gtcctttgtc aaactggaac gactgatata aagctcttct gccaaatcat
     9421 ccataaaaag aatggcatct tccaaaagca attggttcaa tatatacttt tgtcgatctt
     9481 cggactcata aaatccttgc ggttctctag catatccagg tactatttct tgccgactca
     9541 aaaacaagtc aaatgtcagt ctttgtaaaa tatgcagttg gtagccataa cctggtttag
     9601 caatgatctt acctccgttt ttttctacta actctttgat ccgctgcaag taattacgga
     9661 ttgtccgatc agataataac aattcagaag ctaaacgttg actggtgaca tactccttct
     9721 tgtgatcaat caaatatctc aacagcaatt tttccttctg tttcattatc atccactcct
     9781 attccatcat atgttctctt cgatcacctc tgctaacttc tggatgcctg tagggattgg
     9841 gacatacgct tggaaaggga tactaacaac aggctttcca acagattctc ctaatttttt
     9901 gaatttatcc aaaaacatcg tcgtttgcgg gctgatcaga aataaatcaa aatcactatt
     9961 cttaatcata ttatcgcctt ctgtagctga aactgcatct acgagtacct cttctctttt
    10021 ttgcttaaag aaatccgttg tctttgaggc catcatcgag gatgacattc ctgccgcaca
    10081 aataatcaat gcttttttca taaatcaatc actcctttta tttttataaa ccaagcatat
    10141 cttgtaagcg ttttctattc aaaatatata tttccgtagc attgcggaaa aaatgtgtat
    10201 catatcttga ataataattt ctggcaccgt gatatactag tgatagaatt ttttcaggag
    10261 gggatttagt gagtatcaca gtgaatattt tgtatacaag tacaaatgga caagccagaa
    10321 aatttgcaga agaaatggtc gaaagaaaca ttgtcaaagc gattcgtgca gaagaaggaa
    10381 acgagaaata tgactattac tttccactag aagatccaga atctctttta ctgatcgacc
    10441 gttggaaaga tgaagaagca atcgaaaagc atcacaaatc cgaaatgatg gctcaaataa
    10501 acgaactccg taaaaagtat aaattaaaaa tgaaagtcga acgtttcact gatttaccgt
    10561 aaaaagatac acatggaatg atagcaattt atcatccatg tgtatcttca ctttttcctt
    10621 attttttatt cgatctcatc taatgattca aaccgtgttt ttctcttatt tttcggataa
    10681 gtatttcagt caccccaata tacatgattc ctaaacatac cccaaggata ttctcaaaaa
    10741 tgcggatcgt tactgctcct ggaaccccga ataaactggc agtgattgcc aaagcaccaa
    10801 aagaattaaa aactgtttta tatctatagg tagaacaaac acctaaagct agaccaccta
    10861 atataccgac ccagttaaaa ggaataaatt ggctgatacc aataaataaa acacatccaa
    10921 tcaatgagcc aatgattcgg tctactgctc gttctttgaa accaatcgac atcaatccat
    10981 agctagatac aatagaagaa aatgcaaacc ctgcccacat caatcgctga aaagggacat
    11041 actctcctgc aacgatcaaa agactaatgc caaatgcata gatgagcatc caaatttttt
    11101 cctgcgaata catgcctttc aaaaaaatct ctttaaacaa gcttttgtct ttgttctttt
    11161 ctcgatgttt tctgtaaagg agaactgaaa accaacaaaa aaacaaaaat aataagctac
    11221 ttgtttgtgt aaagaaattc ttgttcaacg aatctttagg taacgaataa acaataaaga
    11281 gatatgaaaa gccgtacaaa gaagcattcc ccattttggg atcactagcg gttgccatta
    11341 atatgctgct tagtaaaata aaatgaatca acatgcccat aactgaccat ttgatcagct
    11401 ggacatacgg tgctactaat agtgagaaca tgacgattgc taaacctatg atcgaatggg
    11461 aaatcttata accaaagtca acaaaacgaa tactcatcaa catacagaat aaaaccacag
    11521 cgtatggttt atgcttcgat tcaaataatg ttgtaattat aatgataaaa caaatggcaa
    11581 acaatacgat gaataatgat cgtaaaaaca atgaacaaat atacatgcac ttttcttttt
    11641 tagaagctgc ttctcgtatt ttttgtttta aaataaacgg atctagttgt agagactgat
    11701 aaaaatccac ctgttgtcgt cctttctttt tacttttatt ttttacagtc atctatgaca
    11761 ggttgtaaat actatggaac atcatatggc gttgtcattt tcttgtcaac tatttttcct
    11821 attcatgttt ttataaaaaa acatatcgtt ttcttgtatg attaaatcac cacaaaataa
    11881 acaactgaga gaacgatatg ctttatgaat ggttctatca aaaaaattct gagatcaata
    11941 gacaaaaatt tgatgattac agaggtctct tacgaaacct tacagaagaa aaaaacattg
    12001 atcgttgctg cagttctctt ccctgctcca tgtgtctgta gaaattgtgg ttctgctgtg
    12061 gtagatggaa ataggaaatt aatggtggtg aaaaacaaga agaaggaaac cattgtccac
    12121 tttgaataat ataatcatat gccaatggtt atacgcctaa aaaagcagcg ctatacctgt
    12181 aaaaactgtc gaagccattg gaacgctcaa agttatttta tccgtccaag gcactcgatt
    12241 tcaaatcatg tcagacataa aatcacttct ttactgactg aaaaagtgtc gttatttttt
    12301 atttcgaaaa gttgttaggt atctttgaca accgttattc gcacattgaa agagttgaga
    12361 agctatttac caaagtcttc caaaagaatt ctgtcttgag tgttgagggt tgtgaatttc
    12421 gttctcacgt ttccgttgag gataaactga gttttatttc cgcggatggc gagacagaaa
    12481 aattaatcga gaaaatttac ctacacaaaa aattcctcga ttaacaaact atttccttag
    12541 ctgcactaat cctaaagaaa tagaattctt agttacagac atgaacgcca cctactttca
    12601 gctcaccaaa catgttctac caaatgcgaa attggtgatt gatcgattac ttggtttttc
    12661 cacgcctttc aaggaggcgt aacctttat
//
LOCUS       DACDPS010000068        11734 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.068, whole genome shotgun sequence.
ACCESSION   DACDPS010000068 DACDPS010000000
VERSION     DACDPS010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.068"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            34..411
                     /locus_tag="IWJ07_002054"
     CDS             34..411
                     /locus_tag="IWJ07_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333627.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            complement(591..1886)
                     /gene="purB"
                     /locus_tag="IWJ07_002055"
     CDS             complement(591..1886)
                     /gene="purB"
                     /locus_tag="IWJ07_002055"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAP9333628.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1899..3032)
                     /gene="purK"
                     /locus_tag="IWJ07_002056"
     CDS             complement(1899..3032)
                     /gene="purK"
                     /locus_tag="IWJ07_002056"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAP9333629.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(3016..3504)
                     /gene="purE"
                     /locus_tag="IWJ07_002057"
     CDS             complement(3016..3504)
                     /gene="purE"
                     /locus_tag="IWJ07_002057"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAP9333630.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3797..5104)
                     /locus_tag="IWJ07_002058"
     CDS             complement(3797..5104)
                     /locus_tag="IWJ07_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAP9333631.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(5107..5688)
                     /locus_tag="IWJ07_002059"
     CDS             complement(5107..5688)
                     /locus_tag="IWJ07_002059"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAP9333632.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5804..5901)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(6260..7552)
                     /locus_tag="IWJ07_002060"
     CDS             complement(6260..7552)
                     /locus_tag="IWJ07_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9333633.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7549..8172)
                     /locus_tag="IWJ07_002061"
     CDS             complement(7549..8172)
                     /locus_tag="IWJ07_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333634.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(8175..9011)
                     /locus_tag="IWJ07_002062"
     CDS             complement(8175..9011)
                     /locus_tag="IWJ07_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9333635.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(9028..9915)
                     /locus_tag="IWJ07_002063"
     CDS             complement(9028..9915)
                     /locus_tag="IWJ07_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9333636.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9923..11158)
                     /locus_tag="IWJ07_002064"
                     /pseudo
     CDS             complement(9923..11158)
                     /locus_tag="IWJ07_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
BASE COUNT         3562 a         2607 c         1972 g         3593 t
ORIGIN      
        1 atttggataa tgggttcgag gaggcacttg ccgatggaag atgcaataaa acaaggtcag
       61 ctttttttat cagaattaac agctgaagaa cgttcctgtc tatataatga tttgctgtcg
      121 atcaaagacg tttatttatg caataaagat actagttttt atacattccg aaaaatccgc
      181 cgagcaacca agcgctgtgc tgaatgccgt aaaaggactt ttctttatca gacagaacac
      241 aaaggcgaac tttcaatcag tcagattcat gaacagaagc gtttcaaaaa tcattatcgt
      301 atccagatct tattcaatgg acaagagatg gaatacgctt ttctcgaaaa cttgctgcaa
      361 catctccata accagcattt tagttataat agccaagcca tttacgctta attcctctat
      421 tttttgtatg taaagaggca cggtaaagag gtagccgctt ttaaaatcag gttcctatta
      481 ttgataagaa aatataataa actaaaagaa gcagaaccgc taacggatct gcttcttttt
      541 tggttatttt tgctattcta ttttaattga attttgcaac tcactcttct ttattccaaa
      601 cctactcgtt tgaatatcac gtcaacgttt ttcaaatgat gatgataatc aaaggcatcg
      661 tctatttctt ctttagataa taccgatgtg atggttggat cttcttctaa cagcgggcgg
      721 aactgcactt ggttgtccca agcgtacgca gtttttggct gaacaagatc ataagcttct
      781 tctcttgtca ttcccttatc gatcaatttc agcataacgc gctggctata aatcaaacca
      841 aatgttgcag acatattccg cttcatgttt tcaggaaaga ccgttagatt tttaacgata
      901 ttgccaaaac gatttaacat gtaattcaat aaaatcgtgg tatcaggcaa gatgattcgt
      961 tctgctgaag aatgagaaat atctctttca tgccataatg tgacattttc ataggcagtg
     1021 accatgtgtc cgcgcaccac tctggctaga ccagccatat tttctgaacc gatcggattc
     1081 cttttgtgcg gcatagcaga agaacctttt tgacctttcg caaaaaattc ttctacttca
     1141 cgtgtttcgg atttttgcaa tccgcgtatc tctgttgcaa aacgttcaat acttgtagca
     1201 ataagcgcca tcgctgagaa atattcagca tgtagatccc gagggagaac ttgagtagag
     1261 atttcttgtg gacggatccc caattgttta catacatact cttcaataaa tggcgggata
     1321 tttgcgaacg ttcccactgc tcctgagatt tttcctgctt ctactccttt tgccgcatgt
     1381 tcaaatcgtt cgatgttccg tttcatttct gaataccatg tggcaagttt caatccaaaa
     1441 gtagttggtt cagcatggac accgtgtgtc cgacccatca tcactgtata tttgtgttct
     1501 tttgcttttt cgccaatgat ttccgtaaaa cgtttcaagt cttcccgtaa aatatcattt
     1561 gcttgcttga tgagatagcc ataagcagta tctacgacat ccgtacttgt tagaccataa
     1621 tggacccatt ttctttcttc tcccaatgtt tcagaaaccg ctcgtgtaaa cgcaacgaca
     1681 tcatggcgtg ttaatttttc gatttccaag atccgatcga tatcaaatga tgcatgctca
     1741 cgaatttttt tcacatcttc ttgcgggatt tcaccaagtt ctgcccaagc ttcatctgct
     1801 aaaatctcga cttcgagcca cgcacggtat ttgttctctt ccgtccagat attccccatt
     1861 tcagggcgtg tataacgttc taacatgttt tttctcctct aatcccaaat attcgtttgt
     1921 ttgatttctt ccagcgtttg ctggatgtct tctgtcaaga tagtgatatg tcccatcttg
     1981 cgaccttttt ttgcttgttt ttttccatag aaatggaatt gccagtctgg cttattctca
     2041 atcaagcgat agctttccag cagttgttct cccaaaacat tgaccattac tgcattgctt
     2101 atcaaacgta ctttttctcc taatggccaa ccacaaatac ctcgaatatg ggcatcgaac
     2161 tgactcatag aacaagcttc aatagaataa tgtccagaat tgtgaggacg aggagctaat
     2221 tcattcacat atagcccgcc tgtttttgtt aaaaacattt ctacccctaa aacacctgaa
     2281 aggctcactg cttctgcaat cacacgggca atacgctcag cctcctcgat cacatcctga
     2341 tcaatcgcgg ctggagcaat cgtttcatga agaatattgt tgtggtggat gttttccacg
     2401 accggaaaag tcgtgaaatc tccttgacca tttcctgcca ccatgatcga aatctctttt
     2461 tcaaacggga tccatgcttc taaaacacag gttccttctc ttaaaaggtc catggcagga
     2521 gctagatccg cccgactttt caaaacatac tgccccttgc catcatagcc gcctcgagtg
     2581 gttttcaaaa cacaaggata gccgatcccg tcaatcgcat cttggatatc cgtcggactg
     2641 acgatcgttg cataaggagc aatcacgatg ttattcgttt ccaaaaaaga tttttctaac
     2701 aggcggtctt gcgtaatcgc aagcaagtct gtcccttgag gaataaatga catgggtaaa
     2761 atcgcattta acgcctctac actgacgttt tcgaattcat aggtaatgac atctgttctt
     2821 ctggcaagct ctttcagcgc aaaggtgtca tcatagacac tttcaatgtg ccaatctgca
     2881 acttgtgcgg ctggacaatc gctcacagga tccagcacac ctactttaaa gcccatttct
     2941 tttgcactaa tcgccatcat acgacctaat tgaccgccac cgacgatccc gatcgttgaa
     3001 ccaggcaaca aaggtttaac caagttgatc actactttct ataacagttt tagccaacat
     3061 tgaacgtctt tcagccaatt tagcctccaa ttctaaatca tacatggaaa gcatctgtat
     3121 cgcaagtagt ccagcattaa ttgctcccgc ttttccgatt gctgtagtag ctacaggtac
     3181 tcctccaggc atttggacaa tcgaaagaag cgaatccaaa ccatttaatg ttcgtgattg
     3241 gaccggtaca ccgataaccg gcaaagttgt ttttgatgcg accatacccg gcagatgcgc
     3301 ggctccccct gctcctgcaa taatgatttt gattcctttt tttcgagcgt tttgtgcata
     3361 ttcaaacatc aaatctgggg ttcgatgagc agaaacgacc tttttttcat aagacacacc
     3421 gaattcatcc aatatttgac atgcttcttt cattgtttcc caatctgaag tactccccat
     3481 aatcacagag atttttgctg tcattgtttt tcctcccagt ttttctacat tcatatttta
     3541 acaatcactt tttttgatgt caaagaaatt tcgaatgtta acaaagaaaa acattttatt
     3601 gttcgtgttt attaaagatg aaaaaaagga atgtgacaaa agttaggtca cattcctttt
     3661 tatggataaa cggtgttcaa tcagctgacc ctcggtatta gctcaaacaa acggcaaaat
     3721 acgaggaact atttttccgt ttgtttctct aacaatcagg tcaaaacgct gatttcacaa
     3781 cctttctttt gctctattac ttcttgaagg cttcttccaa tatttcttca tcttttttca
     3841 atccattaaa gatcaaattc aatatgatag ccgtaatgct actcattacg atgccatttc
     3901 cagtgaacat ttgcaatgtt tgcggcattt gactgaataa tgtcggcgta acattgaaac
     3961 ccagcccaaa tccaacagaa acagcaacaa tcaatagatt tttatcattg ctgaaatcaa
     4021 cttccaacag catacggatt ccttggactg ccaccatccc gaacatgact aacattcctc
     4081 caccaagtac cggctcaggg atgatttgtg ctaacgcacc gattttaggg aataatccta
     4141 aaacaatcaa gaagaatgca gagaaataga tgggttttct tgttttgatt cccgaaagtt
     4201 gaaccaatcc gacattttga gaaaagccag tataagggaa tgtattgaaa ataccgccta
     4261 agataacggc aagtccttct gcacgatatc cctttttcaa ttcttcttct ccaacatttt
     4321 tcccagtaat gtcaccaagt gcaaaatata cacctgttga ttctaccatg ctgaccatgg
     4381 aaatgatgat cattagtaaa atcgaccata cgtcgaaagt tggtttacca aaataaaagg
     4441 gttgtggaac atggaacaac ggcgcatctc caatcgcgct cagatcaatc attcccatga
     4501 aagcagctaa gatgcttcct ccgactaaac caatcaatac cgcgattgaa cgaatgaacc
     4561 cttttgccag catttgcacg cctacaatta ggagaatcgt cacaaatgca agtagcagat
     4621 ttgttttgtc accaaaatca gctgctgttg cattacctcc acccattttc tcgattgcta
     4681 caggaatcaa agtcaaacca ataacggtga taaccgttcc tgttactata ggagggaata
     4741 gttttttaat tttagaaaag acaccagaaa ctaacacaac aaagatccca gaagcgataa
     4801 tagatccgta gatagctcct actccttcat tggttccgat caagatcagt ggagctactg
     4861 cttggatcgc acatcctaaa acgacaggca agccaattcc gaaaaatcgg ttaactgtca
     4921 attgcaataa tgtcgctaat ccacacataa agatatcaat tgaaatcaaa taagtcatct
     4981 gttcttgatt aaagcctagt cctgtaccga tcaaaagagg caccgctact gctcctgcat
     5041 acatcgctag cagatgctgc agccctaaaa ccgcagcttt tccattttgt gtttcttttt
     5101 ccacgattat gcgtcctcct caagaaactc aacttgtcca ttcgtcaagg aagcaatacg
     5161 cgcaagagaa acgactctca atcccatttc ttcaagtaac tgacgtccat cttggaaaga
     5221 cttttcgatg acgatcccga ttccttctac ttgcgctcca gcttgctggc ataattcgat
     5281 caatcctttt gccgcttgtc cgtttgccaa gaaatcgtcg ataatcaata ctttgtcttc
     5341 tgaagataaa aatttacgag aaatagaaat cgtacttgtc acttgtttcg taaaagaata
     5401 aacagaagct gtcaataatt cttcatccat cgtcaaactt tttgcttttc tagcgaagat
     5461 cattggaaca tctaattctc ttgcagcaaa taaagctggt gcgatcccag aagcttcaat
     5521 ggtcacaact ttcgtgattc cttgttcttt aaatacttct gcaaaccgtt ttcccatttg
     5581 ttccatcaat acagggtcta cttggtgagt gacgaagctg tcgactttta atacgccctc
     5641 gcctaatata cgtccgtctt tttgaatacg ttcaactaat tctttcactt tatttgctcc
     5701 tttatcattt attaataccg aaataaccgc ttttgtgcaa aaacctacta tgtttctgtt
     5761 aaagaaaaac accctccact ttatcataag aaaagaaggg tgaaatagcc aatcttttac
     5821 ttatagtcca gcatttacgg tactggctag aaactgtcat ccatattatg acgatatata
     5881 agtaggtatg taaaactaat aatagtgtaa attcattttc ttgcaactgt tttgcacgtg
     5941 aaggcagttt gccatatttg aataaaaaat gttgtttcaa atattgaaaa cagtctaaca
     6001 ctactttctg aaactgacaa cgtggtttac ttattttttt gtaaaatcta atgattttta
     6061 atgcattatt cctttttgtt cgggtttaaa tagttatttt tattttctcc tatctaaaaa
     6121 agcagcccac ataaaaagaa ggcatgacaa aaagcatgtc atagcccctt tttccgaata
     6181 aatagtgttc aaaagacagc ctatcggcaa taaggcaaga ttttataaac tacgggaagt
     6241 aatttccgta aatctattct tattctttta agctgacaag ctcgtcaatc actactgctt
     6301 ttcctgaagc aatagaatga tttgctgcga ttcctgtcaa aatcgacaaa ataccatcta
     6361 cgtgagttgc tgcccgattc cacggatctt cttctttttc ttcaccaaat agatcatcta
     6421 ataaacgcgg atctcctccg ccatgtccgc ccgcttcttg ttcgacgatt acctcatatg
     6481 gctcaccgaa catcggatag acatggatgg actgaagaac ggctgctcct tcatcctttt
     6541 tatctccacc tgcattgatg taagactttt cgatcacttt catttcgatc cgtcctttgc
     6601 tgccattgaa agaaacattg aatccttccc acggcatata cgcgtttagc gaatacgtca
     6661 aaatcgcacc agtttcatat ttgaccgtta cgcctagtgt atcttcaata gagatatcat
     6721 cggaaaatac actgcggtca cgaatatatc cactgtcttt ttctgcttct aaatacattt
     6781 ctcgcaattc ctcattggct gacaggtcta tagcaaatgg atcagcttta gctagttcgc
     6841 ttccatagca tcgactgtaa aatatttcta ctccgcgttt ctccgcatta tgcatcccat
     6901 aaaagtttaa tccaccgaat gcaaatacct tttggggacg gctgttcaac caaaaattca
     6961 ctagatcaaa atgatgggta gatttatgaa caagaagccc gccgctattg tgcttatttc
     7021 gatgccatct tctgaaataa tctgccccgt gttgcgtatt taacagccat tcgaagtgga
     7081 tagaaaacac atcaccaatc acatcacttt ctatcagttc ctttatcttc gtgtgatacg
     7141 gtgcataacg ataattaaaa gtgactcgca ggcttcttcc cgtctgtttt tggaccgtta
     7201 ttatttcttg agctttttcc tcatcgatag tcagaggttt ttcgcaaata acatcatatc
     7261 cttttttcat tgcacgaatg atataatcat gatgcgttcg atctactgac gtaataatga
     7321 cttcagccgg ccgtacgtca tccagtagtt gctcaaattg tgatggcata catgtcttta
     7381 cttttttctg tccatacttg ttcacaagta tccgatttgc ataatccatc cttgtttgag
     7441 atacatcaca aaaaccgact agttcacaag tttgatgata cgtagaggca attgcctcat
     7501 aaaaaaaacg cgctctccca ccaagtccga cttgtacata ttttttcttc atttaaagtc
     7561 attctccttt tcttttaagc aaaatactgt gacaaatggc agccctggat acagtaatgc
     7621 agtgagtaaa aaatcaggct gtatacacca gaacagtcca tacaaaaggc ataaatacca
     7681 cagaaacccc ttgacgtttt ttgacaacag ccagatattc cttttgatca aataaacaag
     7741 actatctgct ttatcttctt tgcagatatc aattagcgta ctaaaaaaac tatgggatac
     7801 aaaaagcaac agacacaata gaaaaggaat cagataccaa gctccaatct ttcccactaa
     7861 taaataccaa tcaactgcaa aaataatgac aaacagctga aacaacgtaa tgatgaaaca
     7921 catccgtttg attttccctt tcctccaaca tacctttttt tctgccaagt aatggacaga
     7981 tagataataa gaaggataca tcatcaaaaa ataaatcaga tagacaacac gagcagacag
     8041 cactaatcct aaagtgaaca aacatcctag aactaaagcg aaaagagcat tactgataag
     8101 actggttaaa aaaatccgac agacatagta gttgagcttg taaagaaaca tctcttctat
     8161 tggctttttc atattcagcc cttcagaccg cttgtactga tcccctggac aagatattta
     8221 ttgaagaata agaaaatcag aaaaaccggt agcagcgaca acgtagacat cgcaaacata
     8281 ctcgcgtaag tcgtactaga tccagcgtct gcaaataatt tgatggcaat cgataccgtg
     8341 taagatctcg gcttgtttaa ataaatcaat ggtcccatga agtcgtccca tttccaataa
     8401 aactggatga tgatcgtcgt gatgatcgaa ggtttcagca aaggaaaaat aattttggta
     8461 aagacactat acttgctgca tccgtcaata atcgctgatt catctaactc tttggggatg
     8521 ctgaccatga attgcatgat ctggtaaatg aaaaatgctt gtccaaaaaa atgtggtaaa
     8581 atcagcggca catacgtccc aaccaaattc agccgattgt aaataataaa ctgcggaatc
     8641 aaaatcactt gtgcaggaat catcatcgtt actaacatga cgacgaacca gaatcttttt
     8701 cctttgaaat cgattctcga taatgcgtaa gcaatacaag tcgagctcag aactgtccct
     8761 atcgtcgaaa ctgcagagat gacaaacgaa ttcctaaaga aagtaaaaaa tgaaattcca
     8821 gcaaaccccg cccatccttt tgcgtaattt tcccattgaa aagtctctgg aatcaaattc
     8881 agcgaaccac tcataatctc ttgatccgtt ttaaaagaac ctgcgatcat ccataagacc
     8941 ggataaatca tgactagacc taatagcaaa ataaaaccgt attgaaacca tttcgttact
     9001 gtattattca tttcttcctc ctctcattca cacaacgacc catttttttg atgatttgaa
     9061 aatcagaaag gtgacagctc ccataatcaa cagcatgatc cacgaaatag cactagcata
     9121 ccccatagaa ttaaaagaaa aagcttggtt atacacataa agagcataaa agttcgtggc
     9181 atcattcgga ccaccttggg taatgataaa tgcctgcgta aaagtcataa acgccgcgat
     9241 tgtctgcata accaagttat acaaaatgat cggcgaaaga agaggtaacg tgatcttgta
     9301 aaacgtctgc catttgttcg ctccatcgat ctctgccgct tcataatacg agcctgggat
     9361 ctctttgata cctgcagcaa agatgatcat tgccgaaccg aattgccaag cagacatcaa
     9421 aacaagaggg atcatgacca gtttttgatc gctaaaccag gaaagcgcag gtgcgcctaa
     9481 tcgttccagg aaaatattca ctaacccgtt tttagaaaac aactcccgcc aaactagagc
     9541 gatagaaata ctgccaccga tcaaactagg cacataatac aaagaacgaa agaaagtcgt
     9601 tcctttgata ttcctcgtta aaagataagc aatcaataga ccaaagatca gtttcacagg
     9661 tacagatgca aatacataga gaaaagtgac tcggatggag ttcaaaaaac gggcatcact
     9721 cagcatcttt atataattat ccaagccgat ccatgagact ggatttccca aacgataatc
     9781 ggtgaacgaa taataaagtg acaagatcat cggtatcact gttatcaata gaaatcctag
     9841 gatgaatgga gaagcaaata cgtaaccgac cacattgtct tgatccaaga accgttttag
     9901 tttttctttt ttcatgcctt ccttctgctg ttacttcttc ataaatgact ttttcaacta
     9961 aaagattgta ttggtcttga atttccggat tcaaagcact atcaaagaca ttcacttcgc
    10021 cgttgtccat atcggaaatc atttctaaat aatcaaatgt cgcttgcaaa gttgcatcat
    10081 ctgcacgagc gagctgttct cgaatattcg acatgattgg tacaccacgt tctcctttta
    10141 aaatttcatt ggcttcttca ctgttttgaa agaaatcaat aaacttagca gcttcttctt
    10201 tatgggctga gctttttgcc atactcagca tttgcgaaga acgtaaaggg ataccagatt
    10261 ctccatctgc ttttctgcga ggtggattaa tcaactgaat ctccatatct tcaggagccc
    10321 ctgacaatat ttctgtcaac tggttacttg atagaaacac cattgccgct tctctatccc
    10381 taaccgcctg aaaagacaga tccgtcgtac ttgcagctcc accacctggt tctggatggg
    10441 cccctttgtc taccagattt ttacgcagct ggaaaaaatc tgttagatag tctggatttt
    10501 caaatcccaa gctgttagat agatcttccc gattataaaa attcaatcct ttctcaactt
    10561 gtggtatttg aataatacag ccagctataa aatcttcgaa tcttgaaata gcatagattc
    10621 ctaattcttc agtgatttga tagctgattt gttcaaattc atcccatgtc caatttgaag
    10681 ctggttcagg gatatttaat tgttcaaata tatttttatt gtaagcaatc gcaaacgaat
    10741 tcaccccgtt agacagtcca actaattgcc cgtcgtaagt cgtcgttgct aaataggatt
    10801 catctgcatt agaaatatca attatccctt gctccacata ttcatctaat ggttcaatct
    10861 gattgatgta ttgaggaaat tgattgttca gttggaaaat gtcccagata tcatctgatg
    10921 ctgctagtgt acctaatttt gtgtaatatc cttcataatc ataaaattca taatcgatcg
    10981 tcacatgggg atttttttct tcataaagct cgattacttt tactgttgca tcgtgtcgga
    11041 cttgcgaacc ccaccaagcc atgttcagcg tgattttttt gtcatcttct tgcccacaag
    11101 ccgtcaagaa gaataacata acacttaaag ctaagatgat tctcattctt ttcttcattc
    11161 tctgcacctc tacttccatt gttatttttc aatatactgt atttttatac agaacatatt
    11221 tattaaacga tctttttcaa ggggaataaa aaccaagact tgccttatcg ttctgcctag
    11281 gatgaacaac tcaatacttt caagaaaaat tagatagtgc tgattcttct ttcatcgcta
    11341 aaaacacttc ttgcttcaat gatgggagga taattacgca aagaaaaaag gatatcagac
    11401 aactgcccaa tatccttgac atagctaaat aagcatgctt cttcgtgtgc gctcttccct
    11461 aaaaaacaga aaaataacta gtaattctgt atgaaattag gtacaatata cacgtcgatg
    11521 cacaagcatt gactatggca acttgattgg tcagttcccg ctgacttttc aagccccgac
    11581 aatttagctg ctactaaatt gtcgggtgcc ttttttagtt tttattcgtt tttcattcta
    11641 tagaaagcgc ttcaataaat caagacattt ttttgttaga atgctaataa taacaaaagg
    11701 ttttaactct tcttatgtac attacaggta aaaa
//
LOCUS       DACDPS010000069        11585 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.069, whole genome shotgun sequence.
ACCESSION   DACDPS010000069 DACDPS010000000
VERSION     DACDPS010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.069"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            6..305
                     /locus_tag="IWJ07_002065"
     CDS             6..305
                     /locus_tag="IWJ07_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9333637.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IWJ07_002066"
     CDS             378..1739
                     /locus_tag="IWJ07_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP9333638.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IWJ07_002067"
     CDS             1761..2021
                     /locus_tag="IWJ07_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333639.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IWJ07_002068"
     CDS             2035..2787
                     /locus_tag="IWJ07_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAP9333640.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IWJ07_002069"
     CDS             complement(2881..3486)
                     /locus_tag="IWJ07_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9333641.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IWJ07_002070"
     CDS             complement(3539..4513)
                     /locus_tag="IWJ07_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAP9333642.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IWJ07_002071"
     CDS             complement(4891..5670)
                     /locus_tag="IWJ07_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9333643.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IWJ07_002072"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IWJ07_002072"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP9333644.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IWJ07_002073"
     CDS             7161..8558
                     /locus_tag="IWJ07_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9333645.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IWJ07_002074"
     CDS             8639..9079
                     /locus_tag="IWJ07_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9333646.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="IWJ07_002075"
     CDS             9227..9553
                     /locus_tag="IWJ07_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9333647.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="IWJ07_002076"
     CDS             9554..10303
                     /locus_tag="IWJ07_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333648.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="IWJ07_002077"
     CDS             complement(10383..10838)
                     /locus_tag="IWJ07_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP9333649.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="IWJ07_002078"
     CDS             complement(11265..11540)
                     /locus_tag="IWJ07_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333650.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
BASE COUNT         3656 a         2012 c         2353 g         3564 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacttgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcc
//
LOCUS       DACDPS010000070        11566 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.070, whole genome shotgun sequence.
ACCESSION   DACDPS010000070 DACDPS010000000
VERSION     DACDPS010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.070"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            90..610
                     /locus_tag="IWJ07_002079"
                     /pseudo
     CDS             90..610
                     /locus_tag="IWJ07_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(512..1228)
                     /locus_tag="IWJ07_002080"
     CDS             complement(512..1228)
                     /locus_tag="IWJ07_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAP9333651.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(1278..1487)
                     /locus_tag="IWJ07_002081"
     CDS             complement(1278..1487)
                     /locus_tag="IWJ07_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333652.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            1764..3037
                     /locus_tag="IWJ07_002082"
                     /pseudo
     CDS             1764..3037
                     /locus_tag="IWJ07_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(3144..4175)
                     /locus_tag="IWJ07_002083"
     CDS             complement(3144..4175)
                     /locus_tag="IWJ07_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9333653.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(4182..5012)
                     /locus_tag="IWJ07_002084"
     CDS             complement(4182..5012)
                     /locus_tag="IWJ07_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9333654.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(5009..5815)
                     /locus_tag="IWJ07_002085"
     CDS             complement(5009..5815)
                     /locus_tag="IWJ07_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9333655.1"
                     /translation="MKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLANYS
                     DIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLAANF
                     AGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYPSMK
                     EVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALTSSN
                     VNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(5821..6645)
                     /locus_tag="IWJ07_002086"
     CDS             complement(5821..6645)
                     /locus_tag="IWJ07_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase"
                     /protein_id="HAP9333656.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(6635..7735)
                     /locus_tag="IWJ07_002087"
     CDS             complement(6635..7735)
                     /locus_tag="IWJ07_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9333657.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCVSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            7913..8698
                     /locus_tag="IWJ07_002088"
     CDS             7913..8698
                     /locus_tag="IWJ07_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HAP9333658.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            8731..9114
                     /locus_tag="IWJ07_002089"
     CDS             8731..9114
                     /locus_tag="IWJ07_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP9333659.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            9190..9321
                     /locus_tag="IWJ07_002090"
     CDS             9190..9321
                     /locus_tag="IWJ07_002090"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9333660.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            9290..9526
                     /locus_tag="IWJ07_002091"
     CDS             9290..9526
                     /locus_tag="IWJ07_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9333661.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(9604..10575)
                     /locus_tag="IWJ07_002092"
     CDS             complement(9604..10575)
                     /locus_tag="IWJ07_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAP9333662.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            11209..>11566
                     /locus_tag="IWJ07_002093"
     CDS             11209..>11566
                     /locus_tag="IWJ07_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9333663.1"
                     /translation="MQGVIAMMSKNNTNGRNQFAMLTIDDLVSQDHLVRKIDAALDFE
                     FIYPIVEATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYS
                     FEEKIPHFSTFGKNYVR"
BASE COUNT         3448 a         2659 c         2204 g         3255 t
ORIGIN      
        1 acactttttc ttcgtttcaa ctcaaaaaga gactcacttt tggtaaaatt tagtcgacca
       61 aaacatcacc ctaccaaagg agtcgctcta tggataatca tactagaaaa ttactcggat
      121 taacagataa acatttattt tttgatgaag aatggctgat tgaaaaagaa tataaaggcg
      181 ttcaagcgca gtttattaaa gggagattgg atgactcacc ttcccattgt gaacactgtg
      241 gatcgattcg tattatccgc aacggttcct atacgacacg tactcagatg ttgaaagtca
      301 aagaaaagtt gactatttta gaattaaaac gtacccgttt tctatgctat gattgtgggc
      361 agaccttttc tgcgaaaacg gacttagttg atgaacatca tcaactaaca aaggaactga
      421 agcaggcgat tctcatagaa ctgtacgaaa atcaatcacg taatctgaaa atcagaagaa
      481 gtaccgtcgg ctcaagcgat attggaaact cttactcaaa gattccacga cattggagcc
      541 gctcaaacgg cattatcatc gactgtttaa acgaccaatt agtcaaacag aaatcgtcga
      601 tgaaccgttg tgcacaatgg tgtcctgaac gtacagcgac accttccata tccagagcag
      661 tcgccacatc acggggatgg atgccatcca aattaaaagc aaccacgcca gtatgatgtt
      721 caggatcttg cggtccgtaa atggtgatac cgccgatcgc ttgtaattta ggtaaaacgt
      781 aggcaaccag ttcttgctca taacgatgaa tattctccat accaatctca ttcaagtaat
      841 cgattgctcc tgcaatgttc ggagtaccag cttcaaattt ccatggcagc tctttccatg
      901 aactatcgta aagattgaca aaatctatca tttcaccgcc gaattcgact ggctccattt
      961 gttctaacaa ttgacgtttg ccatataata caccgattcc tgtcggcccg cacattttat
     1021 gcccgctaaa ggcgaaaaaa tctgcatcca acgcttgtac atcaactgtc atatgcggtg
     1081 ctgcttgggc accgactgga ctatcctcaa ctacaacagc ctgttcaacc ctcagccgtt
     1141 tcaagcacaa caaataacct tctggatctg gtttgccgac ctgaagatca tcgatcgtca
     1201 caaaggaatc aattttttcc ttaatcatcg gatgtgcttc aaaaaaatag tctagctccc
     1261 gcagttcaga attgagacta ctccgatttt ccagtgctgc tgatccgctg ctcaaaatgt
     1321 tcagcatcca ctggtctccc aaccgcttga cttatccctt cataaaagga cagttctgct
     1381 gccgtcccaa caccttgttg atagtctgca aaaggaattt tatagccgaa ctgctccagt
     1441 aaataggctt cagtaaatag tgatgaatac tctcgtaaaa catccattca ctgtcaatca
     1501 tcgtcccatc aaaatcaaaa ataagtgctt tggtcatctt ctttccccct tacccaaaac
     1561 aagcaaaagc tgtgtcctgc tctctctaca gtatcagcat aaaaactgcc gtctgaaaag
     1621 tcaaaggtga aagaaatgtg gcgctataat ttctaggact gatagtaaat cattctatca
     1681 gtccttttga gcataaaaaa acaaaacata cagaagctcc gttagaattg aatcaccaca
     1741 acacaatcaa aggagcgttc tgtatgcctg atcttcatct tatcaaacta ttactaggaa
     1801 tcaaagatgg aaacattcaa atcactcatg tcgagaagaa aaaaatacat tcaatcaaca
     1861 gtatcgttgt ttctgccacg ctcattaaaa cgattcagcc ctgtcctcag tgtaaacagg
     1921 tgaatcaaca tgggctgatc gttaagaacg gaaagaaaaa atcgctggtc cttctcaata
     1981 agtgtgcgaa tcaacggact tatcttgctt tggcaaagca acggtacctc tgtcgtggct
     2041 gtcacaccta ttttacggcg aatacctaca ttgttgaaaa aaactgtttt atcgccaaac
     2101 aagtacatta taaaatattg gaggaattga ctgaaaaaca agcaatgacg acgatttcta
     2161 aacattgcgg cgtttcttgg tcaaccgttt cgcgaacact cgcttctctg ctccccatga
     2221 caaacgtgaa aataaattgg ttgcctcgct gttcattggt agatgaattt cgttcattga
     2281 aaaatcaagt cggtccctat tctttttctt gtatggatgg cgatactggg aaattactgg
     2341 atattcttcc ttcacgtaag aagaaagatc tcatttctta ttttatgcaa tttgaacgac
     2401 gtgcacgttt aaacgtaaaa atactcgtaa cagatatgaa cgcctcttat gctttgctga
     2461 taaaagagtg ctttcctcat gccaaactcg ttgtggatcg ctttcacatc gtcaagcacc
     2521 tcattcgcag ttttaaggat atttgactat gagtcatgaa aagcttcgac cgaaacgatc
     2581 caatacaagc caaacactat cgacaagtga aggctttatc tcgacttctg atcaaacgac
     2641 aggacacgct tgtttatgat aaatggacaa aatggcgtaa ttttggttgg gcctatttaa
     2701 ccgaaagtga agtcgtagaa cgtctacttt ctacttctga tgaactacga atagcttatg
     2761 cttactatca agaaattctt caagcttttt atgacaaaga agcggacatc ttctttcagc
     2821 ttgtaaaaac aatgcccaag tcggttccaa gagaactgca tcatataaaa aaagcgttta
     2881 taaactatga atccggcatt cgtttagcct tagagttgcc ttactcaaat ggcaaaatag
     2941 aaaatctgca tacccacatc aaagcattga aacgaatagc ttacgggtta ggatgagaac
     3001 cagaattttc ttaatcaatc atctgatcac ttattaaaca taaaaaaaca tctaaagcag
     3061 aaaatcaatt ctaccttaga tgttcttata tcactctatc agtcctattt gacaaagagc
     3121 cagaaatgtt tgccgactat acactagctt tgttgtgcaa aatgctgagt atcaatttcc
     3181 acagggattg ctagaatatc tttcagctga atgtataagg catcgctaat tttctcttcc
     3241 cgatcgttca accgatccac taaaatttct ttgccctcca gcgtttgaaa aacataccgt
     3301 tgaatgttgc ctaaaataaa tgattccttc catagaaccg gaatcttaac cccctgagga
     3361 accggttcaa attgaatcag ctccggtctt aaataataaa tgcagcgatt gcccttagcg
     3421 gaaaactcgt gggtatactg attcagctca taccattcga aacgattata gttgccgata
     3481 aattccgcaa caaatcggct ctgaggattc tgatacaatt ccttcgaggt ggaaacctga
     3541 gccacccgcc cagcttccat cacaaaaatc cgatcactga ttcgcatcgc ctcttcttgg
     3601 tcatgagtca caaaaatcat cgtcatccct agcgattgct ggatagcgcg caaatcccgc
     3661 tgcagctgtt ttctaattcg ggcatccaaa gcacttaatg gctcatccag cagcaggact
     3721 ttcggttcag tgaccaatga gcgagccagt gccacccgtt gtcgttgacc gccagaaaga
     3781 aactggacat ttttatctgc ctgctccgtc atatccacca atgatagcat ctcaaaaacc
     3841 ttttcctgaa tcacctcagg actcagcttc tttaccttca aaccaaaggc tacattttca
     3901 aaaacagtca tagttggaaa caatgcatac tgttgaaaaa caaagccgat attgcgatcc
     3961 ttcgtaggct tatcggcaat attctcattg tccagcacaa tctcaccgct ctttggttgt
     4021 tccaatcccg tgatacagcg cagcaacgta gactttccgc acccgctggg acctagtaaa
     4081 gtaaccagct cacccttttc aatctgaatt tcatcaatag ctaaaatctg tttttcatca
     4141 aaaaacagat tgagatcatg tatttctaaa aacattttcc gtcacaactc ctttaagggc
     4201 ttctttttcg actgcttctc ttccctcgtc tgcttaaggg acagctgttg cttttgtcga
     4261 taagtcagca taaaggtagt taaagcaatt cccaccataa acagcaaata caaaaccatc
     4321 acagcgctag ccaaatggcc gttttcgttc atccgccgca gcatatagat gcgtaccgtc
     4381 tgaaatcgac cgccaatcaa caggttcgtc agcatgtact ccccaaaaac cgaagaaaaa
     4441 atcatcagag aaactagggt cacaccgata cggatgtttg gaaacagcac ctggataata
     4501 atcgtagaca tcggggcgcc caaggtttct gctgcatcca ctaattcctt cagattaatt
     4561 aagcgcatct ggttgttaag actaagatag acaatcggca acgccgtaat aaacaatgca
     4621 cctaccaaca ctacaaacat tgggatacca gtttcagcat acgttcgaag caaagccgtt
     4681 accaaaatca ctcctggtaa agcatacggc agcaggagca gcttttccag ccaacgattc
     4741 aaacgaggga aatacaaatg aatccaaata atggtgggaa tcatcaacaa cagaatcgtc
     4801 accaagacca cacctgctaa taaaagtgag cgacccaccg cagcaataaa ttccctgtcc
     4861 gtcagcagct gttggaacca ttgcaaggta aaatcactag gtaaaagcga ttttgaccat
     4921 ttagtggagc tggcatacaa aatcgttcca ataatcggca gcaagtaata caggacaaag
     4981 cagaacaaaa tcggttttat taactttttc acgtctgcac caccttgcct ttcttttgaa
     5041 taatcaacat tgccgttacc agcaggacac ctaaagcgac tgccaacgca ctgcctaaat
     5101 taggcttggc gaacacatct cccgacacta aagccgcaat tcgaatcgtt aaaagattta
     5161 cattactgct ggtcagggca tacgctgttt cataggtccc catgccatta gcaaataaga
     5221 tgacaaaggt gctggcaatc gatggtcgca aaaaaggcaa agccaccttg aaccaagcat
     5281 acgctgatga agcgcccagc aattgtgcgg actccagcca ttcctttttc acttccttca
     5341 tgctgggata caaaaacaag acccctaacg gaatttggaa ataggtatac gtgatggtca
     5401 atccccacca agaatacaaa ttgaagtccg ctagaaatgg caaaatcatt ttcagcacac
     5461 ctacattccc caacaaaata ataaaagaga atgccagcgg aatcccagca aaattagctg
     5521 ccaaattgga aacaaaggtg attttctcct gcaatttagt cgatagattc attaagcata
     5581 aacataaaat aaacgtcccg attaacccaa tcacactgga gctgacagca atcagtacgc
     5641 tgttaacaaa ggcctgtgtg taatatttgt tgctgaaaat atcactgtaa ttggccaatg
     5701 tccactggtt agttaaatca ctgtgcaagc tgcacagaac cataaaaagc agcggcagca
     5761 gtaaaaatgc acagaagata aaggaggcag gaaccagaac caagcgtttt ttcatctctt
     5821 tcatcccctc atcaattctt gaatgtcctg cattttctct gacggtgcaa ttcctaataa
     5881 ccagcaaaac aatggagccg ccagcagctg tggtacagtt tgttccttca gcttttggcg
     5941 tttatttgaa atcaaataaa aaggaacttc acgatgtgca gccaagctgc cgccatgcag
     6001 tccgtgcgca tccatcccgt gatccgcagt caccacgact tgataacctt tttgcagcca
     6061 ttcaggaagg taaatagcca ataatctgtc cgcctgatta accgctgctg catattcatg
     6121 agaatcggcg gtaaatttgt gaccgacatc atcaatattc atcgaatgta cgaccaaaaa
     6181 gtctggctga tactgattca ctaaataatg ggcatccgca aataaatgcg aatcagggta
     6241 agtgtcctca ctataaaaga tgccatgctg aatcagtcgc tcctcctgat gctggatgcg
     6301 atcaaccaaa cgattatagg gggcttgatt gtacaactcg ctgtaccagt ggtaagcggc
     6361 ggctcctgtt tttccgccag cttccttaac taattcaaac agcgaccact ctttggatcg
     6421 ctgagcatgg taattactat agatgccatt ttcataggct ggaacgccgg tcatcagtac
     6481 ttcgtataac ggacgtgaat tgctgggcag ctcacttttt accttcaata agcttgcttg
     6541 ctggtgctca actaaatgat tcatgaaccc aagctgggcg acggctgtgt cgtagcgaca
     6601 gccgtctagt ataactacaa tcagtttgtc aagtctactt tgcataggtc agtacctccg
     6661 cctgccaaag ctctggaagc tctacagagg tcttgttcca agcttcattg tcttcaactg
     6721 gcaccgccgc ttcataagca tcctccggca gtaatttttc agctacttct ttaggcagct
     6781 ctaccttgcg aataggtcgc gcatagccct ttgccaaatt gatttgccct tcatcagata
     6841 aaatatattc ccgtgctagc tttgccgcat tgggatgagg ggcattttta ttgattaagg
     6901 ttgtataccc gctaatgatg gagccgtctg ttggcacagt tacctcaaaa cgctcggagt
     6961 caatttggtc acgataattt agtccattga agtcccaaac aactgccaca tcgatttcac
     7021 ctttttcaag atttgctacg ctggcatcaa cggtggacaa acggcctttt tctgccattt
     7081 ttttgtagta attcagacct ggctgaatat ccttttcgtc acctccgttg gcaatcgccg
     7141 cagccaatac agcaaattgt gcttggttgg caatcgtcac atcccccacg ctaacttgat
     7201 agtcaccctc ttgcagctcc ttccagctag tcggtgcttg cttcacattc tctttatcgg
     7261 tgataaagga aatggttccc gtatagctta acaaccaatc cccatcatca tcctttgccc
     7321 aatccggaat ttcctcccaa tagttggtct tataaggtaa ggttaactct ttgctttcag
     7381 ccaacggacc gaagctgata ccgacatcac caatatcggc ggttccattt tttccttctg
     7441 attcaaactt agccaattct tctgcactgg acatatccgt atccacgtga cttaagccgt
     7501 attctttttc aaggtcgttc caagtgccta cccaatttgc ccaagtatcc ggcattccca
     7561 agctggcaat ctctccttct tccttggctt tttctataat cgtatctaaa gagtcgtctg
     7621 ccgcagccga ttgcttcgtt gcttcacctg agacgcagcc tgtcagcagg atacttactg
     7681 ttgctgcaca aaataaaccg atagtcatgt tacgaatttt ttcattcatt ttcattcatt
     7741 cttctcccct ttcgataatt ccattctaac aaagctttgt aatgagagaa tcagtttctt
     7801 gaaaaggttt catgaacaat gtaaaggttg tatatggttt gtaaatttcg acgaattccc
     7861 gttaaccttc taaagctttt gcggtatgat agtagaaaaa aggaggctcc ctatgcagta
     7921 ttatgatcag catctccaca cgtatttttc ctttgattca gaggaaaaat ttgaaaacta
     7981 tttagcctat cagccggagt ttttcgtttc tacggatcac tttgatttaa acaatccatc
     8041 taccggaaaa gatgacattc cggattatcc agcctatata aacaaactgg actctttaaa
     8101 agaaacctat tccaccaaat ttctgcgagg tatcgagatt ggcgttgttc caggacaaga
     8161 agagcggatc gtggattacc tcgctcagca tccttatgat ttaaagctgg taagtattca
     8221 tcaaaatggc cgctttgatt atatggacga tgtggtttta accaaagatc ccattcaaac
     8281 taccaaggaa tattttgatc agatgttgca ggtcttgacg gattttccag aaggagatat
     8341 tttgactcac tttgattatg gattacgccg cttcaccttc acagcggaag aattcgcgga
     8401 gcattttgag gctcagctgc taacgatttt caaaaaggtt gttgaacgag aattggcgat
     8461 ggagttaaat gccaagagct tcttgaaata cggcaacgaa gacctttacc gttatgccgt
     8521 tcctttgtat caaagtgtcg gcggaaagct ctttacctta ggctcggatg ctcatgttgc
     8581 cgaagattat cagctgggct ttaaaaaaat gagtcagctg ttggcagact gtcaggtaga
     8641 aaagttgcta gtcattcaag gaaaagaacg ccgcctgacg ccgctgccga ttttgtgatt
     8701 cctatataat agaagaaagt ggtgaaaaaa atgagcaacc tgtgggacta caatcaagag
     8761 gcacccatcc attatttaat tgcgcgacat tgggatgcgt tgaaaattga ggcggtttgc
     8821 cgatccttgt tagcagccgt tccaaaacaa caactggaaa attttttggt ggctgacagc
     8881 ttgcagcggg aaaaagttca ggcttatttt gcagccttta aagatcagcc tttggaatat
     8941 ttgcatgcgc agtttcatct tttttatcaa gtagccgccc cagatgacta caacgattta
     9001 cgaggacagc tgcaattgac ctttcaagcg gatgaaaccg cctataccgt tttattggga
     9061 atggctcgcc ttggtgatca ggcgaaggtc gagtggcgga ttttcgatat ttgatggcag
     9121 gatcaaggcg tttcagttgc aaaccttcct ctctttcaat tagaaaaacc gcagcaggaa
     9181 gaagtttgtg tgctggtcac cgatgctggt gaaattcgtc tccgcttatt tcctgaccaa
     9241 gctcccctgg ctgtagccaa ctggcgctcc ctagctgagc aaggcgttta tgacaacaca
     9301 cctttcggct cgtgtcatta aggaatttgt tattcaaggt ggcgctttgg atggttctgg
     9361 tgatgaagct gtttccagct atgagggctt tttcgctgat gaagtgcatc gcggacttta
     9421 tcattttaat ggcgccttgg ccttagggga ccacggcccc cacaccaacg gcaaccagtt
     9481 ttttattgtg caaaacacaa aggcgcaggc tgacctgcta atgtaaagca agcatacttg
     9541 gagcttggag gactaccgga gctggatggt cgttatactg tttttggaca ggtataccaa
     9601 gggttagaaa tcgtagcagc gatcgcagct cagccgacaa acagtgaaga tgtaccgatt
     9661 gagccagtgt tcattcgaca aattaacttc gaaaaaacat cgtaaaaaga cactcactga
     9721 cttcagcaaa agctctattc ttgagctggc tgcagctagt gagtgtcttt catttatcca
     9781 taagataaag cttggattca ataaataaat aaatgttttg gttaaagctg tgaccagcaa
     9841 atgaggaacc gtgcgagcca gttgatctgc caatgcaaac atttctttag gtgaaggcgc
     9901 tggcgtgtct gccaacggat actccagtcc caatgcttgg taaccgtgca taccatagca
     9961 gtggtacggt agtaattcta acggtacgaa ccgatcaaga tctgccacaa aacaagcaat
    10021 cgtcagcagc tcggtcaggt tcatattgat acctggaacc acaggaactc gaattcgcac
    10081 ttcgcagtta ggatactcaa ttaaaaagcg acgaatattc ttcaaaattt gttggttatc
    10141 taagccggtc cattgtttgt ggagcttgct ggtaaacatt tttacatcca caaacaatgt
    10201 atccacatag ggagctacct tttggatttc attataagca ccacaaaagc tggtttccag
    10261 tacggtattg attccaatgt atttcgattt ttgcaaaatt tctttagcaa aatccgcctg
    10321 aaccagtgcc tcgcctccgc taatggtcac tccgccatcc gagtgaaagt aaaaaacagc
    10381 atccttctca atctcatcca ttacctcttc agcggtcatg gtctggccaa atcctttttc
    10441 aatctgcttg ctttgtgatt cgggaatcga acaccaggca cagcgtaatg ggcagccctt
    10501 taaataaaca acggtccgta aaccagcacc atcatgtaga gaaccctgtt ctattcttaa
    10561 tactgttcct tccatataga tttttgaatt acacactagt ttaaactaaa acacacaagg
    10621 aaaaacttac catttaatca taaaattagc tcatataaat atactaggat gtaaaataga
    10681 ctggtcaaaa tctggtcaaa aaagaggact aatacaggta gtcctctttt ttgttatttt
    10741 aatattctaa atcatttttt attggtttat tcccttattc gatgtagact caaatcacta
    10801 tatcactaga tatcaaacgg cgtcatacgg aatcaaaaac atcaaatttg acctgttaaa
    10861 tggtcattga ataacattca aaatcaataa aacggagtca gacacggagt caaaaacgct
    10921 gagagtaaaa aatatataca gccttgtagg taaagtacca cagggctgta tattttttat
    10981 ttcagattac cttaaatgat tccatttact ttttatctca ttttttccta ctagttagta
    11041 tcaaatactc tcctattttc tcaataaact atttttggat acatggtatc tgaaacagct
    11101 aaaaccttgt cattacagca aaaaatcacg ttatccagac tgtagacaaa gtccatttta
    11161 tggatgatgt ctacagtctt ttcttttata atagatttat gaaaacgaat gcaaggagtg
    11221 atagctatga tgtctaaaaa caatacgaat ggtagaaatc aatttgcgat gcttacgatt
    11281 gatgatctag tttctcaaga tcatctcgtt cgaaagattg atgccgcgct tgattttgaa
    11341 tttatttatc caatcgtcga agccacttac tccgatttag gtcgcccaag tatcgatcca
    11401 gtgattctga tcaaattggt gtttattcag tatttattcg gtatccgttc catgcgacaa
    11461 accattaagg aagtcgatac caatgtcgct taccgctggt ttttaggcta ctccttcgag
    11521 gagaaaatcc cccatttctc aacttttgga aagaattatg ttcgcc
//
LOCUS       DACDPS010000071        11508 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.071, whole genome shotgun sequence.
ACCESSION   DACDPS010000071 DACDPS010000000
VERSION     DACDPS010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.071"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(79..2154)
                     /locus_tag="IWJ07_002094"
     CDS             complement(79..2154)
                     /locus_tag="IWJ07_002094"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAP9333664.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDIKTDRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKDHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEV
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2156..3073)
                     /gene="glyQ"
                     /locus_tag="IWJ07_002095"
     CDS             complement(2156..3073)
                     /gene="glyQ"
                     /locus_tag="IWJ07_002095"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAP9333665.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRF
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARSVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(3457..4269)
                     /gene="recO"
                     /locus_tag="IWJ07_002096"
     CDS             complement(3457..4269)
                     /gene="recO"
                     /locus_tag="IWJ07_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAP9333666.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFLSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHAYPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKMPEKNMPEKEIPEKEE"
     gene            complement(4412..5311)
                     /locus_tag="IWJ07_002097"
     CDS             complement(4412..5311)
                     /locus_tag="IWJ07_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAP9333667.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYTALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5326..5727)
                     /locus_tag="IWJ07_002098"
     CDS             complement(5326..5727)
                     /locus_tag="IWJ07_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAP9333668.1"
                     /translation="MPMDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQIDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5705..6181)
                     /gene="ybeY"
                     /locus_tag="IWJ07_002099"
     CDS             complement(5705..6181)
                     /gene="ybeY"
                     /locus_tag="IWJ07_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAP9333669.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6198..8390)
                     /locus_tag="IWJ07_002100"
     CDS             complement(6198..8390)
                     /locus_tag="IWJ07_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAP9333670.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNTGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8414..9385)
                     /locus_tag="IWJ07_002101"
     CDS             complement(8414..9385)
                     /locus_tag="IWJ07_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAP9333671.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10090..11469
                     /gene="brnQ"
                     /locus_tag="IWJ07_002102"
     CDS             10090..11469
                     /gene="brnQ"
                     /locus_tag="IWJ07_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP9333672.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
BASE COUNT         3106 a         2419 c         2103 g         3880 t
ORIGIN      
        1 ttatttatat ttataacaaa taaaccagag aaatagccta ttttgtgcct gttctctggt
       61 ttatttacat attagaaatt attttgtgat gatttgatct aaacttgcta gtgacaaagc
      121 catggcagca atttgactta attgcgttaa tcggttctta cgtactgctg gttcttctgc
      181 catgaccatc gtctgatcaa aataattttc gatcagtggg cgaaggctta caagctgatc
      241 ataattttcg ttgatcgttt ggtcagcaaa gcttgttttc attacctcta ctgctttgtg
      301 cagttctttc tccgcttcgt tttcgaacag ttcagggtct acttctgatt gtttttcaag
      361 ctccgctttt ttcgctaagt tcacgacacg tgtcaaagct tccatcgatg gtttgaagtc
      421 ttcatctgca agatgctgct tgaacaattg tgctgctgca aatacttttg tcagatcttt
      481 ttgttctgcg ttcaaaacag cttcgatgac atcatgacgg atattcttcg ttgtcaataa
      541 ttggcgcaga cggcctttga taaagtcgat cacctgttgt tgtcccgctg acaagccaat
      601 gccaaaacga tccgtatctt tactgataac ttcactaatc gtttcttgca gaacagacaa
      661 aggaaaggcc cattttttcg attcgatgat tcgaacgaca ccatatgctt gacggcgcaa
      721 agcatatgga tcgtttgagc cacttgggat catcccaact gcaaagaaag aaaagatact
      781 atctaattta tcagcaatcg ctaaaacaga acctactgta gtctcaggta aatctccttc
      841 actggatata ggcatgtaat gttcacggat cgctgttgct acggctggtt tctcgccttt
      901 aagcagagca tatttttctc ccatgatccc ttgtagttca gggaattctc ctaccatgtt
      961 ggttactaga tcaaatttat agatttcagc agcgcgttgc agatccatca attcttcctc
     1021 agacagtcct acttgttcgc ctatgatgcg agcgatgact tgtacccgtt gcattttctc
     1081 atagatgcta ccgatttttt catggaaagt aacagttttt aatttttcaa cgtattggtc
     1141 gattgaagct ttcttgtctt cattataaaa gaattccgca tcttctaaac gagcagtcaa
     1201 tactttttga ttgcctcgta ccacattttc caattttacc cgatcgccat tacgagcaga
     1261 aataaaatga ggaagcaaca taccttgatc gttccgtact tcgaaataac gttgatgttc
     1321 tttcatagaa gtgactagta cttcttcagg aacagataga tatttttctt cgaagtcgcc
     1381 agaaaagaca gttggatatt cgactagatt gttgacttct tctagtaaat cttcatctag
     1441 atcaatgatc cagtgatctt tttctgctaa ttcgtttgct tgtgtgatga tcatttcttt
     1501 tcttttttgc ggtgaagcaa taacaaattg ttcatttaat ttttcttcgt attcttttgc
     1561 atgcgcaaat gtcacatctt cgcctaagaa acgatgacct cttgaagctc tgtctgtttt
     1621 gatatccaag atctcaaacg ggattacttc gtcatctaac aatgccacga tccagtgaat
     1681 aggacggata tattcaaaat cgtattctgc ccaatgcatc gttactggga aagtgaggct
     1741 tttaatgaca tctgctaaac ctgtcaatac ttcttttgct tctttgccgt gagtgtattt
     1801 cgttacatag acatattcca cgccttttaa ttctctaaaa gtgatagcat ctgtcgtcag
     1861 accttgtccg cggacaaatc cttccgctgc tttcgtccag tttccatctg cgtccaaagc
     1921 aatttttttg gctggtcctt tgacttcttc ttcactgtct gcttgacgtt ctggaatgtt
     1981 tgttactttg attgctaaac ggcgcggtgt agaaaatgtt tcaatcgtat cataagtcaa
     2041 ttggttttca tctaaaaatt ttgctgtttt ttcttctaat tgtttcatgc ttggtgtgac
     2101 aacatgagca ggcatttctt ccagcccaat ttcaagtaat aagtcttttg ccatcttatt
     2161 cagcctcctt cacgttttct ggatttgctg tgtttttatt caataaaggg aatcctaatt
     2221 tttcgcgttc tgccacaaag attttggcta cagaacgagc catgttgcgg atacgggaaa
     2281 gataacctgc acgctccgtt acggatactg caccgcgggc atctaataaa ttgaatgtat
     2341 ggctgcattt caaaatataa tcatacgctg gatgaacaag attttcatcg atacagcgtt
     2401 tagcttcttt ttcaaaacga tcaaagttgc ttagtagcaa gtcttggtcg cttacttcaa
     2461 aagaatattt ggaatgttca tattctggtt gtttgaagat ctcgccatat ttcacgccat
     2521 cagcccattc taagtcatag acactttcaa cttcttgaat atatgaagca agacgttcta
     2581 gtccataagt aatctcagaa gtaactggtt tacaaggtag tcctccgact tgctggaagt
     2641 acgtgaattg tgtaatctcc atcccatcaa gccatacttc ccaacccaca ccggcacaac
     2701 ccatcgatgg gttctcccag ttgtcttcaa caaaacgaat atcgtgttct aatggatcaa
     2761 ttcctaaaaa acgcaagctt tctaaataaa gctcttggat attttcaggt gaaggtttca
     2821 ttacgacttg aaattgatga tgctgataca aacggtttgg gttttctcca taacgcccgt
     2881 cagctggtcg gcgggatggc tctacataag cagcattcca tggttccggt ccgattgccc
     2941 gtaagaatgt atatgggctc atcgtacctg ctcctttttc tgtatcataa gcttgcatca
     3001 gcatgcagcc gtttgatgac caaaattttt gtaatgttaa aatcatatct tggacggtca
     3061 attttttact catctgtttt gcccctttca atttttaagc gttgctgtca ataagaatac
     3121 aggaaagaaa acgacaaaaa gtccctatgt ctgattacag acatagggac gaataatttc
     3181 gcggttccac cctacttccg aataaagatt cggcagcttc atttatcgta ctcgcaagtg
     3241 cctcttcaaa aatcgtttgg gtccggcttc cactctcccg aactcgctaa accacgtgtt
     3301 ttttactgat cttggtcaat gtacgtattc attttgcagt tttcttgttt atataataga
     3361 tcctctcagt gaacgatcta tttaatctac ggttaataat agacggaagt ccctgtattt
     3421 gtcaaatagt tttgattttt ttgttgtatc tttcatttat tcttcttttt caggtatctc
     3481 tttttcaggc atatttttct ctggcatctt taatacgtgt gcccaagact tcatttgatc
     3541 aatgaacttt ttgcttttta gatgaatacc gacatattca tcatacaact ggtcaatgag
     3601 gtcacggatt gcctgcttcg tttcttcttt caagtggatt ccactgatct tatcataaga
     3661 aatagccgaa aacattcgaa cgaaataaac agcgcgagga tatgcatgat aacggtgttc
     3721 gtccattggc cagtgtttct cgcatatcac gccgccatat ttggaagaat agtcaaattt
     3781 tccttttgtt tccccgcata cgacacagtg cgtccaaatt ggcgcgattc cgaagcgttg
     3841 cagtatttga acttcaaaga tattcgtgat gatttctgca tcatctcctt ggtccatccg
     3901 ctgcagagct tgttgcgtaa attggaataa gtgcgggtca tattgatgat cttcgattgc
     3961 tgcatctact agatttagca aatacgtacc ataaccgctc aaaaagatat cttcttggat
     4021 gttccgaaac gcatggacgt ctttgacact gcttaaaaac gacaaacctt cacttagaaa
     4081 attaccgata tagactgctt cggtaaaggg gagcaaagcc ggagctaatg gctgattttg
     4141 ccgatggatt cctttgatat aaaacatcat tttgccagaa gattccgtaa agattttgac
     4201 taatttatct ttttctttgt aattacggct agataaaatc aagcctttcg attctgcaag
     4261 ctggctcata ttctccctcc taaaaaataa atctggaaaa gcagattctg ccgatccaga
     4321 ttatttatgt tacgatcttt cgctgttttt ctcaacgtac ggatttttat ccagtttatt
     4381 tctataccta acctataaga agtccagaat atcagtactc gtcttttcgg tagccgaagt
     4441 cttgcagata gacttttttg tctcgccagt ctttttggac tttgacccaa agttctagaa
     4501 atactttgtc tcctaaaaga ttttcgatat cctttcttgc tttcgttcct acttgcttca
     4561 gcatttttcc acctttgccg ataatgattc ctttttggct atctctttca acgatgatcg
     4621 tggcctggat atggatttta tcattctcgt ttcgcttcat cgaatcaaca acgactgcga
     4681 cagaatgagg cacttcgtct cgtgttaaaa acaagacttt ctcacggatc aattcagaaa
     4741 cgatgaagta ttcaggatga tcagtcactt gatcatctgg aaaatattgt gggccctctg
     4801 gcatttcact gactaataca tccattaagc gttcgacatt gtttccttct gtagcagaga
     4861 tcggaatcac ttgtgcaaaa ggcatctgcg tcgaataatc ttcgatgatt cccagcaact
     4921 cgtctggatg gatcttatcg attttattga taatcagata aactggagaa tgcacgtttt
     4981 ttagacgttc aataataaaa tcgtctcccc ggccgcgttt ttgatcagca ctgatcataa
     5041 ataagactgc atccacttct ttcaacgcag tgtaagctgc ttctaccata aaatctccta
     5101 gacgatgctt tggtttatgg attcctggtg tatcgataaa aatcagctga gcttctggtg
     5161 tagtatatac tccttggatt ttatttctag ttgtctgtgc cttgtcactc ataatggcaa
     5221 ttttttgacc cacgattcgg ttcaataatg tggattttcc tacattcggg cggccgacga
     5281 ttgcgacaaa ccctgattta tgttctggca tatacgttct cctttttaat taaacaacaa
     5341 atgatatatt ttagggacaa ataaaatcaa cccaatgatg acagcaaaac aagcggtcac
     5401 taaaactgct ccagctgcca tatctttgat ttttttccca atcggatgaa aatgaaaatc
     5461 cgtaaacatg tccactacat tttcaaaaac agtattgatg atctctacta tccaaacgag
     5521 aaaaacagct aaaagcagcc ataaccactc tatccgatca atctggaaaa tcacgcccat
     5581 aaataaagca aggataccga acgcaacatg tttcctcata ttgcgttctt cctcaaaaac
     5641 ggttttgact ccttggacag caaattccac ggacatcagg aaatgtttgt ttttctctac
     5701 gtttttatct tttgagtcca taggcatcca agatctcttt ctgtaaacca aacatttctt
     5761 tctcatcttc tggtgtcata tggtcatacc cgttgatatg caagaatcca tgtaccgcta
     5821 aaaagccaag ttcccgatca tatgaatggc cgtaagcttc tgcttgttca gcagcacgtt
     5881 caatagagat catgatgtct cctagtacac gaggcaatgg attctctcca tcttcaaaaa
     5941 tcactgggat ttcgtcttcg ccttcttctt ccagagcaaa actgatgacg tctgtcggtg
     6001 catcttttcc acggtaatca cgattgatta cttggattgc cgcattgtcc atgaaagtaa
     6061 cagacatttc tgtttcctct ggcagcttta aataagatgc tgcaaattgc agcaagtcat
     6121 cgatttcttt cattttctct tctgagactt ggtttgtttc atccataaag gtaatatcca
     6181 ttatacatcg cttctttcta ttcttcttgt tcttccttta tcttctcaac ttctgttttc
     6241 attttaggat atttgatccg agaatggaag gtgctactta aaccgctgat caatgatttt
     6301 tccacttttc ggatttcttt caaggtcaat ccactatcgt ctaattgacc atcggaaata
     6361 cgctcttcga taagattatg gacaaaactt tcgatttttt cgctcgttgg atgatccatt
     6421 gcccgtactg cagcctcaca actgtcagca atattgacga tccctgcttc acgactttgc
     6481 ggttttggtc caggataacg gaattgctct tctgttactt ccggattgcg ttctttcgct
     6541 ttgatgtaga aataacgcat tagcgtggtt ccatgatgct gtttgcaaat atcgatcacc
     6601 atttgcggca tattataatc ttccaatatt ttggctccat ctgtcacatg tccaaagata
     6661 atatgtttac tgtcttctgg caataagaaa ttgtgcgggt tttccgctcc tgaaggcaag
     6721 ttctcaacga aaaagctagc atgtttgatc ttgccgatat catgataata gcaagctact
     6781 cgagttagta aagaacgtcc gccgatttct gctacagcgt tagcacttag attagctacc
     6841 atcatactat gatgatacgt acctggcgct tcttccagca actgtttcaa taaaggatga
     6901 tttggattgc tgagttcatt taatacaatt acactgtcat cattgaccat caattcaata
     6961 tatgggtgta gaccgatcgt cagcaagaat gagaagagtg caccagtgaa cccgcagaca
     7021 agcattaacc aagtcgttgc atcaccaaag ctcattcctt gatagacgat caaaatgaca
     7081 tccatcatca caggaaaaat aatgacccac atggcagcag ataatccttg ttctgagatc
     7141 cgtttacgtc tgacaatcgt tgccatgagc ccagagaaaa cataagacat caagataatc
     7201 gttagaaagt tcgtgccaat cgaatcataa aaaatgaaaa gtgcggatac tgcttggaaa
     7261 ataccagcca tgatccctgc tcttcgattc aagaaaaagc ttaaaacaag tggtgcaaaa
     7321 gcagcgggat aaaacagcgg gatataagta gcttgttccg tttggaataa ttggaaaaat
     7381 ttcatcagca ctatgctgac tgacatagct gtcacataga aaagaacgta ttttgttctt
     7441 tctcctgtat tttggaattg aagtgaatta taaatcagta cgccgatttg aagcaaaaca
     7501 gctaaaacca tggcaattag cggaaaaatc gaagttgttt gattagtcag tcctagtaat
     7561 tccaattttt tcatagctga agcatcaatt tgattacctt cacgaacaat gatttctcct
     7621 tggaagatca tcactggttg tacagcttct cgtgcggatt gtttcaattc ttctgtcttt
     7681 ttctcattca atacgtcatt cactacgata ccttgatcca ctaagtagcg aatcatttgt
     7741 tgttgtgctg gtgtgacatt caaatattgg atttgctctt ctgcttcttg tttaaaagct
     7801 tccaattcac tttcacgaac ttgtttgctc atctgttcgt caatgagctt cagactctca
     7861 tccctcacag tatgaatctc agtttcagtc atttcaaata tcgtcgaata aaaattgttc
     7921 ggtagttttt gataaaacac aacattttct gcattcacat tttcaaaatt tttcttcagc
     7981 gcggcgatcc gctcttctgt agtaggcttt gggatatttt catcttcctt tgccttatca
     8041 atcttttcgt cataagattt gttgatggca tcattcgtct tatcgatcaa ttcaaatagt
     8101 tgcttgatcc gattgttctg gtcatctgca agatcttttt ggtaagtgta ttctggagtg
     8161 actgcttctg ctgccagttt tcttttttgt tcggtttctt ccgtgttctc gatcgttttg
     8221 ttcgcacgga tactttcttc tgctacttgg ccttctctga aatcattggc cttttgtttg
     8281 acactactaa atgtaacgcc gcagataatg ataaaaaaca agaacagcag gaaaggcaat
     8341 gctgcctttc caagtttttc ttgtagtttc tgcagtgttt tattcaacat gtgcttatcc
     8401 tcctaagcaa agcttattct tgatggagat ctgccttttc ataagctttg atgatttcgg
     8461 caactacagg gtgccgaaca acgtcacttg cttcaaaatt aacaaaatcg attttcttga
     8521 tttgctttaa tgttcgttct gcatgaacaa gaccacttgt tgtccctcga ggcagatcaa
     8581 tctgactcgt atctccattg acaatcattt ttgattgata accaagacgt gtcaggaaca
     8641 ttttcatctg agcaactgtc gtgttctgtg cttcatccaa aataacgaat gcatcatcca
     8701 atgtccgccc ccgcatgtaa gctaaaggag caatttcgat cacgcctcgt tccatcaatc
     8761 ggttcgtatg atctaatcca aaaatttggt ataaagcatc ataaacagga cgcagataag
     8821 gatctacttt ttcttttaga tccccgggaa gaaagcctag attttcccct gcttccacag
     8881 caggccgcgt caaaatgatt ttttgcactt cacctttttt caaggcagcg attgctaata
     8941 ctactgcaag aaaagttttt cctgtcccgg ctggtcctac gccaaatacg atatcatgtt
     9001 tggctaccga ttcgacatat ttcttttggc cgctgttttt tacccgtata ggttttccat
     9061 tacggtcttt gacgatctct tcctcataca tctcaacaaa ataatctaat gtgccttttt
     9121 gccccatttt cagtgccgtc acgacatctg gtgtacttac atggattcct cgtttgatca
     9181 attcctgcaa tgtccgaatg actgaggcag ccaaggaaat ctgggattgt tcgccaatga
     9241 tctgaatgat ttctccacgt gtatggatgg ttgtttctgt tgtttcttca atcagtttta
     9301 tgtgcttgtc gtgtgcacct aaaagcatgc tgacatcatc agcagccgtc aatctaatct
     9361 ctaaagaact agactcttcc gtcaaaaata gacatcccct ttttttcgat tcattctttc
     9421 ctattttacc atactcagaa gaaactatat agcaaataag aaaaggttct aaaagagttg
     9481 cttaaccata agtaccaaga aacgttaaaa aatagctgct tccattctct aaaggtctcg
     9541 tttgaataaa aaaagctgat catgtaagct atgcataaga tagaggacaa gtattccgtc
     9601 taacttagca agtattctct gggtattttt cttgtttcaa aaaaagaaaa caaaatattt
     9661 gaaatattct tgcatttcaa aaaaaacagg tgtattataa cgtcaacaaa taaatataca
     9721 gcaaagcgtc gaggaaaaca gtaggctatt cagtgattta ttcccagtga gtcatgtaag
     9781 tgagaaatga caataaatcg aatagacgaa catcatttcc gagtcaatgt cgaaaacgta
     9841 gatcattgtg gctgcctcca ttatcaggcc agcggataac tttatcgaaa agcgtccgtg
     9901 aatgaagaag cttttttaag cgaaaaaagg tggtaccgtt ctagaatcta gagccctttt
     9961 cccaagtgga aaagggcttt ttttatattt taaggtggtg gttttcatgg aatctgatat
    10021 tagtggtatt tctagtttct gcaacgttta ttttgttact caaaatgata attattggag
    10081 gaaaaattta tggaagaaac aaaaacttat tcatggaagc aattattgat ggtcagttca
    10141 cttatcttcg ggatgttctt cgggtcaggt aatctgatct ttcctgtcca cttaggtcaa
    10201 atggctggat caaactggat cagcgctggt gtaggatttg ccatctcagg cgctcttttc
    10261 ccattattag ctatcttagc tgtagtagtg acaaaaagtg atggtctgta tgacttagct
    10321 aaacctgtcg gaaaatggta tgctgccttg ttcttgatcc ttgttcattt aacgatcgga
    10381 ccattctttg gtacaccgcg gacagcagca actgcttttg aaatggctgc taaacccttt
    10441 ttgcctgaaa aatatgattc attaggtatg tttttattct ctgctctttt ctttttactt
    10501 gcttatttcc ttactgtaca tccaacacgt ttggtcaaat atgtcggaaa gtatttaaac
    10561 tccttgtttc ttgcattatt agctatcatt tttgtcgttt ctttattcca acccatgggg
    10621 aatctaaacc aatctgctgc agattcttat cagacgaacg ctttgacaaa tggcgtttta
    10681 gaaggataca acacggttga tgcagtagct ttacttgctt taagtgtcac ttttgtccat
    10741 gcagttcgtg atcttggttt taaagataaa aaagtcacag aattaacagc aaaagctggt
    10801 agcctgagcg ttgcgttaga agtcatgatt tattttggtc tggtccttct cggtgcgatg
    10861 agtttgaata agatgtcttt atcagaaaat ggcggaactg cactttctca gatcactaat
    10921 ttttatttag gtaacttcgg tgctagcttc ttaggggtaa tggtcacatt aggtgtcttc
    10981 actaccgcta tgggactcgt tgtttctttt gcacaagatt tccataaact gttccctaaa
    11041 gtcagttata tgacatggct gcggttaacg acttttgtct cttttgtcgt tgcgaatgcc
    11101 ggattggata atatcatcca atggtcttta ccagtattga tgcttttgta tccattgtca
    11161 cttgcgttaa tcttgctttc cttaacagca aaattcttcc aaaagagtcc atttgtctat
    11221 caagtaacga tgttcttttc tgccgttcca gccgtattgg atatgttggc aaattcgcct
    11281 gcattagtga gtcagcaaac tgtcgtagca agcttactag gattttatca tcattatgtt
    11341 ccttttgctg cattaggact tggctgggtc gtcccaacgt tgcttggtta cgctggcagc
    11401 ttgctctttt attatgtata tcgcgtatca ggatataaac aagaagcaag tgagttacca
    11461 gaagaataac caaatcaaac acaatttaac ataagagata ttaaacag
//
LOCUS       DACDPS010000072        11316 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.072, whole genome shotgun sequence.
ACCESSION   DACDPS010000072 DACDPS010000000
VERSION     DACDPS010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.072"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            266..535
                     /gene="rpsN"
                     /locus_tag="IWJ07_002103"
     CDS             266..535
                     /gene="rpsN"
                     /locus_tag="IWJ07_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP9333673.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            550..729
                     /gene="rpmF"
                     /locus_tag="IWJ07_002104"
     CDS             550..729
                     /gene="rpmF"
                     /locus_tag="IWJ07_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP9333674.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            761..1138
                     /locus_tag="IWJ07_002105"
     CDS             761..1138
                     /locus_tag="IWJ07_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333675.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1159..1308
                     /gene="rpmG"
                     /locus_tag="IWJ07_002106"
     CDS             1159..1308
                     /gene="rpmG"
                     /locus_tag="IWJ07_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP9333676.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1332..2315
                     /locus_tag="IWJ07_002107"
     CDS             1332..2315
                     /locus_tag="IWJ07_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP9333677.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2345..2467
                     /locus_tag="IWJ07_002108"
     CDS             2345..2467
                     /locus_tag="IWJ07_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP9333678.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2485..4008
                     /locus_tag="IWJ07_002109"
     CDS             2485..4008
                     /locus_tag="IWJ07_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP9333679.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            4032..4271
                     /locus_tag="IWJ07_002110"
     CDS             4032..4271
                     /locus_tag="IWJ07_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333680.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4464..4925)
                     /locus_tag="IWJ07_002111"
     CDS             complement(4464..4925)
                     /locus_tag="IWJ07_002111"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9333681.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5120..5338)
                     /locus_tag="IWJ07_002112"
     CDS             complement(5120..5338)
                     /locus_tag="IWJ07_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9333682.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5456..7072
                     /locus_tag="IWJ07_002113"
     CDS             5456..7072
                     /locus_tag="IWJ07_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP9333683.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7313..8653)
                     /locus_tag="IWJ07_002114"
                     /pseudo
     CDS             complement(7313..8653)
                     /locus_tag="IWJ07_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(8929..9084)
                     /locus_tag="IWJ07_002115"
     CDS             complement(8929..9084)
                     /locus_tag="IWJ07_002115"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP9333684.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(9380..9712)
                     /locus_tag="IWJ07_002116"
     CDS             complement(9380..9712)
                     /locus_tag="IWJ07_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9333685.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(10226..10417)
                     /locus_tag="IWJ07_002117"
     CDS             complement(10226..10417)
                     /locus_tag="IWJ07_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9333686.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(10511..10897)
                     /locus_tag="IWJ07_002118"
     CDS             complement(10511..10897)
                     /locus_tag="IWJ07_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9333687.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
BASE COUNT         3751 a         1789 c         2175 g         3601 t
ORIGIN      
        1 gctacttaaa acggtatttg tgtttgattg gactaataaa agtgaattct tatttttcag
       61 agcctaaact gcatattctt ttaaatgatt tgattatctc tatgtaccga taaattcgtt
      121 ttcaaatagt tatctattca tatgagtttt atttcatgtt atttattgtt gacaagagca
      181 actcttgttg gtaaagtatc agtgtcaaaa cgtaacgatt acgatttgag gtgtctaaat
      241 caaaataaaa ggagtataaa ggtatatggc aaaaaagtcg aaaattgcta aagcaaagaa
      301 acaaatggcg atgattgaaa agtatgcaga taaacgtcag gaattaaaag ccgcaggaga
      361 tcgcaccgct ctcgctaaat tgccaagaga ttcaaatccg aatcgtctga ggcttcgtga
      421 tcagacggat ggtcgtccaa gagggtatat gcgaaagttc ggtatgtccc gtattaagtt
      481 tagagaactt gcccatcaag ggttgattcc tggagtaaaa aaagcaagtt ggtaaaagga
      541 ggaaatatca tggcagtacc agcaagaaaa acatctaagg cgaagaaacg gttaagaaga
      601 actcatcaaa ctgtagtaaa accagaaatt tcatttgacg aagcgaatgg tgattataga
      661 cgtagccatc atgtatcttt aaaagggtac tataatggaa agaaagtaat taaaggagct
      721 tcaaaatagt ttaatatata acttttagga gggaatcaaa atggaaatta tttacccacc
      781 tttagttgaa gaaggattaa agtattatct tgaaacgacc cagcaatcgc tagataaatg
      841 cactttttat cgttcgatgg tagagagagg aattattact gaaacgggtt taccgaccca
      901 acaggcaatt gaaaatggat tagtcaaaga ttactatgag gaccaaggct tatcatttga
      961 tgatttttta agaatctatc caatttttga agagtatgac gaggagttat ttcaatgtat
     1021 cgatggttat tgggagatac ctgttgatat gaaagaaaat ttagtttcac aattggaatc
     1081 tggcgagtta acttttgaag atgcacaaca aattcaagca tatcttgaag atcgataagt
     1141 attgaaggag gaagatttat gcgtcaaaat attattttgg aatgtgtcga aactggagaa
     1201 cgtctgtatc tcaccagcaa aaataaacgg aataaccctg aacggttaga attaaaaaag
     1261 tattcgccaa aattgcgcag acgggcaatt tttaaagaag tgaaatagct aagtggtaaa
     1321 aaggtgacaa aatgggcata ccagttacaa tcgtttctgg gtttttaggg gctggaaaaa
     1381 caacgctaat caatcaagcc ttgcaagtct caccctaccc cagggaagaa attattataa
     1441 ttgaaaatga gtttggtgaa gttgggattg atcacaaatt attagttcat agcgaggaac
     1501 gggtgttaca actaaataat ggatgtatgt gttgcagtct tcgcagtgat ttattagcaa
     1561 tattgatttc tttactggaa acaatagaag aacatcaaca accaatttca caaatcatca
     1621 ttgaaactac aggtatagca gatccccaac caatcattca aacactttta acagcgcctc
     1681 aactaagagg aagagtatat atcgatagtt tattgactgt tattgatagt gacagttttg
     1741 aacttattga aagagaacca caagccctta agcaacttgt catggcagat cgcatttttg
     1801 tgtctagaaa acaaaacttg aatttatctg cactaaaaaa attacagaga gaaatcaaaa
     1861 gtattaaccc tttatccgat attcgaatgt tcacacatag tgaaacgatt caaaaaaagg
     1921 atttttataa tttgaaaaaa tttaatcatc ccttagtttt tgaaagtgat tcatcagaaa
     1981 atcactataa tggcaaatac tatcatcaac accatcaagt acatgagttt aaatcaattc
     2041 tcttaacttc agatcaagac ataaaaaaag attttttact ccaatggatt gactggctga
     2101 ttttaaccaa ccaagggagc ctgtatcgtt ttaaaggatt gattgcttta caagagcagg
     2161 attttatagt tgccatgcaa gaggtaaacg aacaggttaa ttttcaatac accactcgac
     2221 aaaaagacca aaccactatt attctaatcg gaaaaatgtt ggatgaagaa aggatcaaac
     2281 agagttttga taaattactg gcggaaagca aataatgata agtagtgatt gggggtaatt
     2341 tctaatggag aaaacggata tatctagtgc atatcgacga cttaaaagcc cgaatattaa
     2401 aactagaaaa agggcattaa aaattatcaa agaacataaa cgaaataaaa tgaagaaatt
     2461 agcataatta aggaaggaaa aagaatgaag aaaatcagtg taggacttat tgggatagca
     2521 gctctaggat tattgggagc ttgctccagc actaatgatg caaaagtatc gaatgataaa
     2581 gatggaaaat tagaaattgt aacaacattc tatccaatgt atgatttcac caaaaacatc
     2641 gtcggcgatg aagcgaacgt ggatttaatg gttccagcgg gatcagaacc tcatgactat
     2701 gagccctcag ctaaggatat ggcaaaagct catgatgctg atgtttttgt atatcataac
     2761 gaaaacatgg aatcatgggt accaaaagct aaagaaagtt ggaagaaagc tgggcctaat
     2821 gtagtagaag gaacaaaaga catgatttta ttgccaggta gtgaagaaga agatcatgat
     2881 cacggagaag aagatcacca tcatgaacta gatcctcata cgtgggtttc acccaaaatg
     2941 gcaattaagg aagtttcaaa tatcaaggat cagttagtta aattgtatcc gaaaaaagca
     3001 aaagtatttg agacaaatgc tgaaaagtat ttgactaaac tgaaacggtt ggatgctgac
     3061 tataccacta gtcttaaaga agcgaaacaa aaaagttttg tgacgcaaca cgcagccttt
     3121 ggatatttag ctttggatta tggattgaaa caagttccta ttgctggact aagtcctgaa
     3181 gaagagccat cttctggtcg attagctgaa ttaaaagagt atgttaaaaa aaataagatc
     3241 aattatattt actttgaaaa gaatgctaat gataaaattg caaaaacttt ggccaacgaa
     3301 gctgggataa aattggaagt actgaatcct cttgaaagtc ttacaaaaga acaaatggat
     3361 aatggtgaag attatgtttc agtaatggaa gacaatctaa aagcgttaga aaaaacaact
     3421 atggttgctg gtgaagaggt agttccagaa aaagaagcga aggatgaaaa gacagttgct
     3481 aatgggtact ttaaagatgc cgatgtgaag gatcgcgaat tgagtgatta tacaggcgaa
     3541 tggcaatctg tttatccgtt gttaaaagat ggaattttgg atgaagtttt cgattacaaa
     3601 gcaaaaatca ataaagacat gacagctgcc gagtacaaag attattatac tactggctat
     3661 aagacagata ttgatactat caacattaaa gacaacacca ttgattttgt agtcaacggt
     3721 gaacatcatc aatacacata caaatatgtt ggatacaaaa ttttgaatta tgaaaaagga
     3781 aaccgtggtg ttcgttttaa ttttgaaact gatgatgcag gtgctggtcg ttttaaatat
     3841 attcaattta gtgatcatgg aattgcacca agtaaggcag agcatttcca tattttcttt
     3901 ggtggagaaa gtcaagaaaa actctataat gaattgcata attggcctac tttctatcca
     3961 gcttcattgt ctaaacatga aattgctcaa gaaatgatgg ctcattaaaa ttcaattttt
     4021 ggaggaaata catggtaaga aaagtattat tgtctatgga ttccaagaat attgatgctt
     4081 atcggcgtta tcgatttgca aagcaaatga gattaaatgg gagtacatta gctgatattg
     4141 ctgcaacttt acatgttagt attgacacag ttcatagagt attaaaacgg gatcctgatt
     4201 gttgttttat caaggtcaat actggaagaa gtagacgttt agcaaaacgg cataatagct
     4261 tggccaatta attgataata ccagagtaga tcatttttcc cttatttgga tttgatctgc
     4321 tttttattat gtcttgaatt gtcaaatcaa gcgcaacact ttatcaaaga agacttcata
     4381 aggtgttctc caatctaaac actttctagg tctgttattc agtttttcaa tcatctgatt
     4441 tatcgttgag ttatctaccg tagctatctc tttcgatttt ggcaagtatt ctcgaatgag
     4501 tctattcgta ttttaatttg ttcattgttg ccaagggcat gaggatctgg aaaataaaag
     4561 ggcatattat tcaatgcttc tgtaactgcg aaatgattgg agaattcttt tcctctatct
     4621 ggagtgatcg ttttgaccgc ctcatttgga atactgctta aaagttcaat cattttctcg
     4681 ctcacataag cggctttctt tctggggacc ttctttcctg ctaaaaggaa gcatgatttt
     4741 ctgtctgtca aagtcactaa acaagtacca ccagttttgc cagctacaat atcggctttc
     4801 caatgaccaa aatcacttct atcatctgcg gattttggac ggccatggat cgaatgagta
     4861 aggcaaatct ttccacgaga ctcgacatga ttttttgtat gtcttgtttt tcctcgatgg
     4921 tgcaatgaac ggatcaggcc tttattgccg tgtgagaggg gttctgtatc gaaaagatca
     4981 ttgcaaattg cacgttagat cgtgataaaa ctaattgaaa agtccatatg ttcatgcttc
     5041 aaacgattcg agatttcctc tggcgaccac tgaaaatcga caaataaatg acgaacaatt
     5101 gaccaaactc acggatcatt tagaatgtga taacgtccgc atttttttct tcgataagca
     5161 taattttttt ccgctttgga aggtgaatag tttttttctt gtttcttctc agttcatgta
     5221 aaatagtaaa cgggctatgc tgaatgtcgt tgccaattat actaatagaa aatccttctt
     5281 cgtgaagcag aaagattctt tctcgttcga gtatagtaag atgacgatac tggctcataa
     5341 ttattttcct tctaggagtg ttctcgacaa acttatacta gttggatatc gattatgact
     5401 catttttatg tgttgcactt aaagtgtaaa ttcaaggtat agaaagaggt tccctatgaa
     5461 aaaaatacct attacaatct taactggcta tcttggttca ggaaaaacga tacttctaaa
     5521 tcatattctg tacggtgatc acggaaagga aattgctgtc attgtaaatg acatagggga
     5581 agtaaatgtt gatggggtat tagtcgacaa gaaaggtttt aaaagaactg gagaaaaact
     5641 tgtttcgatg ccgaatggat gcatttgttg tacattacgc gaagatttga ttgttgaact
     5701 agatcaatta gtgcaatttt ctgacttgaa tcaaattgtt attgaagctt caggaatcag
     5761 caaaccaatt ccaattattc aagcaattat attagctaaa aaccctttag acattgattt
     5821 gacccaacga ttagagattg gtgccattat tatagtagtg gatgtaaaac gtttttggga
     5881 taatttcgaa acgggtgaaa agattcagga aagaaaagct gaaggtaatg tagttgaaga
     5941 agaacaggat attcgtggct tgttaattga tcaattcgag tgttgtaaca tattactttt
     6001 aaataatagt gacttagtgt caaccaagac gatttacaaa attaacggtc taattgctaa
     6061 gttacagtcc aatgccaaaa taattaggac aattaaaggt gaagtagact tatcgaaaat
     6121 tctaaataca aatcttttta atatagatga gacttcctat tcagtgggct ggttcaaaga
     6181 gttagagctt ggctatcaaa atcatattcc tgaaacagaa gaatatggta tctcaagttt
     6241 tatctatcga aatcggttac tccttgacgc acaaaaattt gcccatctta cggataaaaa
     6301 ttttcctggc aatattacaa gagcaaaagg gtttctttgg tttgcccaac atagtgattt
     6361 ttcgacgatc tttgaaacat cgggtagcta taaaagtatt gaatcattaa attattgggt
     6421 agctagttta cctaaaattg agtgtcaaat gattatacaa aaaaatcccg aattgaagga
     6481 agcctggcat ccgttgtttg gtgaacgcat taataaacta gtttttattg gtatgaagat
     6541 ggatcaacaa tgtattaagg aacaacttga gggttgttta actgattctc ttaatgcaga
     6601 taatttccga aaaatttcac catttccatg gaaaaagtct aatatgttta gccagatatc
     6661 gttaatgaaa tttttaagaa aagctggtgt caagataggc gatgtcataa ttattggcat
     6721 tttgattatt acctctttta ttcccttatt tgtattctct atagaaaagg agaatgaaac
     6781 aactgaagat gctatcaaat atgccatcgt tcgaattgat ggcaaggaaa tggatcgctt
     6841 cgatttggat aagatcaatc ataaactcgt gaattatcat cctgcgaagg gacaatataa
     6901 tattgttgag attaaagatg gaagaattcg agttaaagaa gataatagtc ctgatcaaat
     6961 cgcggttaga actggatgga tctcaaaacc cggtcaaacg agtatttgtt taccccataa
     7021 gttagttatt agtattgaga aaaaagaaag cacagattac tacatttact aatctggaaa
     7081 tagatgagga ggtttttaat aagaaaaaga tattattatt aataatagca ggggctttat
     7141 taagtgtgtt aagcgcttgt ggacaagaaa aagaagcggt gacaacaggc acaagtatct
     7201 ttaacgggac ctgcaatggt ttagttaaca gtcggttaga ttacttattt tggctcttcg
     7261 tcaatgagtg ttggtgaaga aattcaatag aataatcgag atactcacac ggctagacag
     7321 ctatcttgtc tagccgtgtt gttgttctgg ctttagtcag ttgggtagta ttcttagttt
     7381 tcttaaaata ttttcctcga tataaaaaga ttcgttctcg gtagttttga aagtttctat
     7441 agccataggc aattcgtttt agtactttga tatggttatt caaacattct atagggccgt
     7501 tggaataggg aacgcgcaac gctcgtttga tttctgtttg gtatttcttg aaggtcgtga
     7561 acactggttg gtagtgctct ggcaactcct tgtagtcttc ttttaggagc gcgacgaatt
     7621 caggaacatc ttgtcttctg ataacggaaa gaaaatcctg gtagacttcg tagcctcgct
     7681 ttaaggaatc atcataagat aatagccgat cgactatctc tgcttctgtc agatgatctc
     7741 gaaatgacgg acgccagtag cgatgctgac ctttcaattc ccacgcattc ttttgaagta
     7801 gtttccagta tttctttaag tgcttcgacc gacgatcccc ttttttaaag gtcttcatta
     7861 cttgaattcg gtggttggta aaagcccgac caatgtgttg gctaatatga aagcgatcgg
     7921 cgacgattcg ggcattcgga aaacactctt tcaccaatgt gcgatagggt gtatagaaat
     7981 ctgaaaccac tagacgcact tgttcacgga cttttatagg gtagcgcaag aaatagtcgc
     8041 gtaaaggatt gagttgccga ttttccacaa tatcaatcag ttgatgggtc ttgccgtcca
     8101 tcatgacaaa gctcatggct cccgagacat ttttcactga tttgaattca tcgaaacaca
     8161 ccacttctgg taacagctgg ctcaaagaaa tggttttagg ccgaaggctg cgaagtattc
     8221 gaatgacagt ggtcggagaa atgtgcttca tacgtgcgat caaagacata gaaagaggtt
     8281 cgcttaatag ctcaagaatg gcttgtttga ctctgcgagc aatggagcag cgacgatcac
     8341 taactgaatc ttctgctaaa aaagtctttc cacagtgttt acatttgaag cgctgtttgt
     8401 taatccgcag aatggtcttg tagttactga catcattcaa taagatggat acttttttcc
     8461 agccctattt aatgatcgtt tgttcatttt gatagtggcg atgaaagcat tcctccggtt
     8521 gataggtcaa tgtgccatca aaaacaaaac atgtttcgtc attgattcgc tctttgcgaa
     8581 aggcatcttc atgaaagata agatttaggt ctagaatatc tagtaactct ttggtaaact
     8641 ctgaatagga catcttgaaa cactcctttg aatggttttc gtcgacttta ttctaaagga
     8701 ttttaaggtg tctgtttgcg tttattagaa aaaagaaaaa gtattggcaa cagaaggcgt
     8761 caccaacact agatattata gatcccttat tttcttcgct tcaaattgtc aaggagtcat
     8821 cataaatttt gtatggattc ttttgtttgg gtagtagtct tctaaataat cttggattaa
     8881 caccttcatc tctattttac ttttatattt gttggagtcg attaattctc atttgattga
     8941 tcgatgaaac gcttcaatta ctgcattgtc aacaggagtc ccagtttggc tcactgaatg
     9001 tgtgatgtgg tggtcgttca acagctcttg ataaataaaa gaccgatgct ggctacctct
     9061 atcggaatga attaccgtag gcaattctcc ttaataaatt tgtaatggac ggacgacaat
     9121 ctcttttttc atgtgtgaat ctaccatata gtaacgtaga atacgagttg ctaagtcaat
     9181 ataggtggac aaatagaggc gaccgtcatt acatggaatg taagtaatat ccatcaccca
     9241 aacagcatca atttggttct gttcaaatta tcgattcaac atttttttac catttctttg
     9301 attgtttttt ctctaccatg agaagatttg cgtcgatgaa atcctcttgt gcataaattc
     9361 atttcacgca taagaacagc tacaacacgt ttattgattt cgattccttc atcgtaaagt
     9421 ttttgagtga tccctggaga tccgtatcga tctttgtgtt cgtagaacac tcgtcttcct
     9481 cttttagtcg tttattttcc tgttcaagaa tgtagaaatc tgctttatca ggtaatcttt
     9541 ttccttgtcc agcaaaagca tttttcccgt atcgacgata ttctgaaacc catcgggaaa
     9601 ttgttggacg agagatattg tattcttcag caattttctt ggtagttact tttttctcaa
     9661 ggatatcctg tgaaatcttt agtttaaatt tacgatcata tgtttttctc aatagagttc
     9721 attccttccg gtaacgtaac tgatagtcaa gaaaagtgta tcatagccca acttagtatc
     9781 agggtttgtc aactaaactg tggaagttga tagataagag tttttaacaa ccaaaattct
     9841 cttgactatt tttgaaatca gacaaatttt gattggacac atagttgaat aaatctaccg
     9901 tcgttacgga aggtaaatcg catgcttttc aattctagag gagaagtcct cgttgaagta
     9961 ccatagccca gttctggatt cgacctcctg cccacttcat acgtaaataa agtaatttta
    10021 ggagtgagac tgtgacaaaa gttattttta agctaaaaac gaattaaaaa acgttgataa
    10081 ttaaaatcat tcgttttaga ttctatgaat cattatagaa cgctgtaaaa tcgatacttt
    10141 ataattataa agaagatagt ttcaaattta ttgttcgaaa tttgaaacta tctttttact
    10201 tatgtcacag actcccttag ttgagttatt ctttaatttc gaatttcgag atagctagat
    10261 atattgcaag catacattct tgataacaat cttgatcata ttttccagtt ctccaggatt
    10321 gcttatgaat caaagagcta aatcttttga gaattgcctc aacagcttct tcatttccag
    10381 cctttgctaa aatgaaatct tcttttaaag aattcataaa tctaccttct tcccatggac
    10441 ttataatcta tttctttggg aaggaggaga acgagcaaag aagcatacaa aattagttat
    10501 tatgcagata ttaaaccagc attttttgaa tttttttgta aagtcgatgt tttaaatttg
    10561 tcgcaccctg tcttgtaatc cccagaacct ttccaatttc tttatcagtt ttatcaaaga
    10621 taaatttttc gataagaaaa atttgttcag gttctttcaa caaaggaagg atttttgcta
    10681 attttatatc ttcaacaagg agagtcacat ttaaaacatt aatcggttgt ttaaatttta
    10741 tagtatcttt agtaaacaaa tatggtggga gttgatcaag ttgttcatgt ttttgaattt
    10801 cttcttgttt tttataataa gtattagcgg tgttcaaaat gaccttttca aagaatcgaa
    10861 tcaactgggc atttacgcgt tcgtcgttat ctgacatatt gcacacctcc tccattaaga
    10921 tttaaaggga aaagaggcaa aaaacacgaa aagaagctaa tgcaattatg ctgcgctgcg
    10981 aataacttct ataagctcac atgcattgat aggactgctg atgtttattt tcaaaaatgt
    11041 ttttttagtt gataatggat agttatcttt atacttagaa aagaaaattc atatatcttc
    11101 ttaaatatca aacatgtcct tatggctaag aaaatgtctt gagcttttta cgtataataa
    11161 atcttttcta aaacctcctt ttggataata ttcttattcg tgtatagatt gtatttttta
    11221 tttcttcccc cttcataatt aacaactaga aaagagttcg aatagacacg tatttgaacg
    11281 aaaattttta ttggattccc aaattggaat atttag
//
LOCUS       DACDPS010000073        11261 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.073, whole genome shotgun sequence.
ACCESSION   DACDPS010000073 DACDPS010000000
VERSION     DACDPS010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.073"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(27..1490)
                     /locus_tag="IWJ07_002119"
     CDS             complement(27..1490)
                     /locus_tag="IWJ07_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP9333688.1"
                     /translation="MKKTSKCLSALLLVGQVFGYTPAVLANEQGNNQLTNSQSATITE
                     ETTTNSTGEENQPETPTEDTIDTPDNPEMNAPPTGSEEQPETPNQPDKPEDNQQPEEE
                     PSEEPNEQPSDESSQTPQKPSTETEAEPPTKTERPQKAPEQSVTVPMNDVPQAQSIGS
                     EETKNYHQAPSAPIQELVPSSTQDNEGTIHFEKDASVETFIRKIGESARKIGQENDLY
                     ASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGKGVSFATQEDLGNGTLYTTQAT
                     FRQYESYEESLKDYAKLLKEGLVGNTSFYDGVWKSKAKTYQEATKFLTGRYATDTSYD
                     KKLNGLIETYDLIKYDKEVAGPQLSKEGYMVPLKNYSISSSFGWRGEEFHRGLDMAAN
                     QGEPIYASKAGTVIEASYHTLSGNYVTIEHEDGSVALYAHQQEYCVNVGQTVEQGQII
                     GYVGSTGNSTGPHLHLELALDGSLTKDKLINPETVLF"
     gene            complement(1554..4571)
                     /locus_tag="IWJ07_002120"
     CDS             complement(1554..4571)
                     /locus_tag="IWJ07_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9333689.1"
                     /translation="MFKRMATWLAIFVTFLNPMLGSVTAFAETINESNSTEEVMKTEE
                     SKSSEEAISTETTSETTEETTTQDTTEDVSPPNVMTQEVPEGYEKYFITIEDIQKATS
                     FSEQERLAQESEEWQAILEKNAQTRATNLTLTENGRKDVNMTRITPEGTMYTHDYMVR
                     WQIDGEDVFCIQEGAITSAGIQYDVTPLENLITTSAIRKKISLIGYFGYYSSGSMDNY
                     VLAQTYIWEVLGGSFVNYGTIGQSNYEAFKQTVDRKINNLLEDPSFDSQTVTVKVGEE
                     VEVKDTKGVFSQWDGSVVENTANVQVRKSGNSLFIKANQNSNDGRISVSSLDPNSRGV
                     GSSFAYVDPVAQNLGKLYVPDPNRAGLTIKVLKNGDFKLTKLDAVTGKKVPNVTYEIK
                     RHDTGETKQYTTDAKGEIALKEIPAFYEKDGKYVQNKLDVKEVKAPAGYILDKQTHTI
                     TIEPNKEVTLTAKNDLQQFIPEGKKVKEVYNAEESWNQGKPVYDTVPAENIEFDIIAK
                     TDITAPDKETVLVKKGEVLDSVKTDKDGHWKANKKFYNGDQNKYQLIEKNVPDNYRQP
                     SEEQTTFSVAYGDNTTPVLTFELDVIDNLLKTGEVDFNKVDSINHLQLPGAKFLIEGM
                     TAHNKNVSFVYETKEEPEKLKLPDGTYKFTEIVFPEHYGQASGQTETILVTVKDGEVQ
                     PITVKNEEIVVPEEDQPEISTLFVTTTGGKDFNPKKDQELVDKVSQVFPKSAVGTEKY
                     WVTQFHKITADGKVTVLDTVKETRKVTDEKETFDVTFNYKADMVSDGDTIVATHIVYN
                     DKEEEEEFARHFDLTNKDQTVTAKEPKIETRFITVNGDQTFDPDKDQELIDHVSQEFP
                     ESTVGETKYWVTQFHKIDKNGKDTVVGTVETARKVSKASEEFKVSFDYKAGTLKDGEK
                     LVATHIVYKDKDHKEEEARHFDLKNEKQTLTAKGIKKETPTTPVKQTPTSTPKQSLPK
                     TGETVNDVLIALGLFLFVGVGAVLVRRKMKQSN"
     gene            complement(4722..5561)
                     /locus_tag="IWJ07_002121"
     CDS             complement(4722..5561)
                     /locus_tag="IWJ07_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9333690.1"
                     /translation="MKMLKVLALGIVVGGVSLAVGTNAEAAENKVWTPRSAEQIKAEI
                     KGNEYTIVWGDTLSAISQATNITVQKLADMNKITNVDLIYAGNKLVFNGNVASVQNSA
                     GETVAQTVIQEKDKVDPNQPVGQGTTSNTQTTPNTTTPTTPAGDQTGTTGGTTTPSTP
                     ENNGGTTQTPTQPGNGGNTGGETTPSNPSTGGGTTTPEEPSNPGGGDQGGNGGTTTPE
                     EPTIVVGEIGNSGKVFNTVDEAYDWANAEVSDPNSQWYNKGFMVDVIIYSDGSQKHTV
                     DFY"
     gene            5706..5951
                     /locus_tag="IWJ07_002122"
     CDS             5706..5951
                     /locus_tag="IWJ07_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333691.1"
                     /translation="MQLNNLNEIHDWAQNNLLNRKQAAKITGQNYDAFSSSLRRNNIK
                     PFLEIGDEPGPSIIRLYLREDIEKYAKQLEEKRQKRQ"
     gene            6014..6436
                     /locus_tag="IWJ07_002123"
     CDS             6014..6436
                     /locus_tag="IWJ07_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333692.1"
                     /translation="MNKKYIVSVGSIVLIVLAIIAFSVSNKTETQPQNTVQSTTTSQS
                     SDHSVYPSLNSLKETHGQDLIDLGSSQITTVLFEKKNIAFITPKEGTDLEFSIKVTNT
                     KENLFSIPDIQYTPNKLKIGDTFGLAKVNEQYFAYSLK"
     gene            complement(6530..6847)
                     /locus_tag="IWJ07_002124"
     CDS             complement(6530..6847)
                     /locus_tag="IWJ07_002124"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333693.1"
                     /translation="MPVKGTSNFDFEIFYVDDEICGVFNKQKYTKEQAIEAWKNELFD
                     PEEEISFVVDESFVGWYHGNAEEEEVHYAGWWLEEGEPREQSVPVWVISENPLEEIEK
                     LEK"
     gene            complement(6874..7080)
                     /locus_tag="IWJ07_002125"
     CDS             complement(6874..7080)
                     /locus_tag="IWJ07_002125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333694.1"
                     /translation="MNWLKNEAELYVFHEEMQEKHLVPNVRNYLFWLEEKNDAQVFGK
                     SAQSPKIKLQLTSKQWNEHEWYIY"
     gene            complement(7061..7243)
                     /locus_tag="IWJ07_002126"
     CDS             complement(7061..7243)
                     /locus_tag="IWJ07_002126"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333695.1"
                     /translation="MTKCERVSVTISFIALLLSVISLLNTAIFIGQVEKQKEISLSEW
                     RYEEVNEGMNNELVEK"
     gene            complement(7257..7607)
                     /locus_tag="IWJ07_002127"
     CDS             complement(7257..7607)
                     /locus_tag="IWJ07_002127"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333696.1"
                     /translation="MKKSYKVFENNEIKEQEAIILSAQTPEEALEQALPFLVESQNIV
                     VEELPEFDSKIVFSFNCKKESEISVSPEFYCRSDISVEGLVMCASAFIEIFANHLQIS
                     VKELLDLLQEITNE"
     gene            complement(7633..7935)
                     /locus_tag="IWJ07_002128"
     CDS             complement(7633..7935)
                     /locus_tag="IWJ07_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333697.1"
                     /translation="MLSKQEKKYIRQKMYAMIGNRAITELSSEELLQVQQLAQIIGSN
                     SIFDSNPLPEMTLETLTAKRYKELRAIGYRMIDIRRALRISDTKFREWRTSNGLAN"
     gene            complement(7938..8171)
                     /locus_tag="IWJ07_002129"
     CDS             complement(7938..8171)
                     /locus_tag="IWJ07_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333698.1"
                     /translation="MTISVGNKVKYIGTAIPRYTNKLVVVKNILVNGLILEYPKEDRR
                     KVVVEGNGTWNMNSFICGFTEVEVTNDNENRSG"
     gene            complement(9157..9479)
                     /locus_tag="IWJ07_002130"
                     /pseudo
     CDS             complement(9157..9479)
                     /locus_tag="IWJ07_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9602..10717)
                     /locus_tag="IWJ07_002131"
     CDS             complement(9602..10717)
                     /locus_tag="IWJ07_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333699.1"
                     /translation="MATKRFTATRKYNELYYQFPKVFITSEKYRSLSDKAKIAYMVFR
                     SRLDLAIQRNQVDSQGFVYFEFTEQELANALNCCRRSVNTIKKELKEYELLEIEDMGF
                     DQQTGKRNKARLYLGELDTTENDVYSLGPVDSTSVDSAQGKEEEESVDNLAIFDDFDE
                     KNPCENSAQLFNNSSNNLDTENLDTKIDTTSPVEMADDWQKEFIAHYSESTFVPKDVL
                     TWIVRSSAEWREAKEQLNMIYDSKRIIEKRQRKYNQTHGLSDGSEYRILGETFAEDLS
                     KTYQRCIQNEKIARHEGKAIQNRKGFYFRALLNFWECCVRELNLDPMATKLSDQAEYS
                     LLQAKRSCSYEDLETQIYGLSQLPKISMHNWLEELDT"
     gene            complement(10723..11121)
                     /locus_tag="IWJ07_002132"
     CDS             complement(10723..11121)
                     /locus_tag="IWJ07_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333700.1"
                     /translation="MVKNKFRATDVLTQATFHMPKLFFIKSSKYYAMTAQAKYAYSLL
                     NEYLEESLIEKRIDEKKHVYISYTNQELAILLNVSEEEIEEIKNELEDYDLLTIEFGR
                     IYLKELIATMEDVRVYQHQNSFLIYGQDEV"
BASE COUNT         2944 a         2328 c         1742 g         4247 t
ORIGIN      
        1 ctccttatca gaaacagcct tattctttag aataagactg tttctgggtt gattaatttg
       61 tcttttgtta aactaccatc taaggcaagt tctaaatgta aatgtggtcc tgtactattt
      121 cctgttgaac ccacataacc aataatttgt ccttgttcaa ctgtttgtcc tacgttcacg
      181 caatattcct gttggtgcgc ataaagagcc acacttccgt cttcatgctc aattgtgaca
      241 taatttcctg acaaagtatg atacgaagct tctatgaccg ttcctgcctt gctcgcatag
      301 attggttctc cttgattggc tgccatgtct aatcctctat gaaattcttc tcctcgccaa
      361 ccgaaggaac tagaaattga ataatttttt agaggtacca tatagccttc tttgcttagt
      421 tgtggaccag ctacttcttt atcatatttg attaagtcat aggtttcgat taacccattt
      481 aattttttat cgtagctagt gtctgttgcg tatcttcctg ttaaaaattt agtcgcttcc
      541 tgataggttt ttgccttact tttccaaaca ccatcataaa aagaggtatt tccaactaag
      601 ccttctttta acagtttggc atagtctttt aagctttctt catagctctc atattgccga
      661 aaagttgctt gggtcgtata aagtgtccca ttgcctaaat cttcttgtgt cgcaaaagag
      721 acgccctttc cgttatgtgt gcctttgatt ccaaatagat tgtagtttgg tgcctgagcc
      781 aattgacttt gtccgcttgc ggattctaaa atggcttgcg cgatcataac agaagcgtat
      841 aaatcatttt cttgtccaat tttacgagca gattcaccga ttttacggat aaacgtttct
      901 acagacgcgt ctttttcaaa atgaatagtc ccttcgttat cttgtgtgct ggaagggact
      961 aattcttgta tcggtgcgct tggtgcttgg tggtagtttt ttgtctcttc tgaaccgatt
     1021 gattgtgcct gcggtacgtc attcataggt acagtcacac tctgttcagg tgccttttgt
     1081 gggcgttccg tttttgttgg cggttccgct tctgtttcgg tacttggttt ttgaggagtc
     1141 tgtgagcttt catcagaagg ttgctcattt ggttcttctg acggttcctc ctcaggttgt
     1201 tgattgtctt ctggtttgtc tggttgattc ggtgtttctg gttgttcttc agaacctgtt
     1261 ggtggagcgt tcatttctgg attatctggt gtatctattg tatcttcagt cggtgtttct
     1321 ggttgatttt cttctccagt ggaatttgtt gtcgtttctt cagtgatcgt tgcagattgc
     1381 gaatttgtca gttgattgtt gccttgttca ttagccaaaa ccgctggcgt atacccaaat
     1441 acttgaccta ctaataacaa agcagataaa catttactcg tttttttcac aagattgcct
     1501 ccattcttca atcaatcatc tagagacagc tgattgctgt ctctagtcat actttaattg
     1561 ctttgtttca ttttacgacg aaccagaaca gcccccacac caacaaaaag aaacagtccc
     1621 aatgcaatta acacatcgtt cactgtttct cctgtttttg gtaagctttg ttttggtgtg
     1681 ctcgtcggcg tttgtttgac aggggttgtc ggcgtttctt ttttaatgcc ttttgctgtc
     1741 aatgtttgtt tttcattctt caaatcaaag tgacgtgctt cttcctcttt atggtcttta
     1801 tccttataga caatatgagt tgcgaccaat ttctccccgt cttttaacgt gcctgctttg
     1861 tagtcaaagg atactttaaa ctcttcagac gctttagaaa ctttacgtgc agtttctacc
     1921 gtaccaacga ctgtatcttt tccattttta tcaatcttat ggaattgagt cacccagtat
     1981 ttggtttccc ctaccgtaga ttctggaaat tcttgcgata catgatcgat caactcttgg
     2041 tctttgtctg gatcaaatgt ttggtcgcca ttcactgtaa taaagcgtgt ttcgattttt
     2101 ggttctttcg cagtcactgt ttgatcttta ttggttaaat caaaatgacg agcgaattct
     2161 tcttcctctt ctttgtcgtt atagacaatg tgcgttgcga caatcgtgtc accgtctgaa
     2221 accatgtctg ctttgtagtt gaacgtcaca tcaaatgttt ctttttcatc ggtcactttt
     2281 cgtgtttctt ttaccgtatc taatacagtg acttttccat cggcagtaat tttatggaat
     2341 tgcgtcaccc aatatttctc tgtcccaacc gcagatttag ggaatacttg ggacacttta
     2401 tccactaatt cttggtcttt tttcgggttg aaatcttttc cacctgtcgt tgtcacaaac
     2461 aaagtagaaa tttctggttg gtcttcttct ggaaccacga tttcttcgtt tttcacagta
     2521 attggttgta cttctccgtc tttgaccgtt actaaaatcg tttctgtttg tcctgacgct
     2581 tgcccataat gctcagggaa cacaatttcc gtaaatttat acgtaccgtc tggtaatttt
     2641 agtttttctg gttcttcttt tgtttcatac acaaaggaca catttttatt atgtgcagtc
     2701 ataccttcaa tcaggaattt cgctcctggt aattgtagat gattgattga atcgacttta
     2761 ttgaagtcta cttcacccgt ttttaacaag ttgtcaatca catctaattc aaacgtaagc
     2821 acaggcgtcg tgttatcgcc ataggccaca gagaacgtgg tttgttcttc agaaggttga
     2881 cggtagttgt ctggcacatt tttttcaatc aattggtatt tgttttgatc gccattgtag
     2941 aattttttgt ttgccttcca gtgtccgtct ttgtcagttt ttactgaatc caagacttct
     3001 ccttttttca caaggactgt ttctttatct ggcgcagtaa tatctgtttt ggcgataata
     3061 tcaaattcga tattttctgc aggaacggta tcataaacag gtttgccttg attccaggat
     3121 tcttccgcat tatagacttc tttgactttc tttccttctg gaatgaactg ttgtaagtca
     3181 ttttttgcag ttaacgtcac ttctttgttt ggttcaatcg taatcgtatg agtttgtttg
     3241 tctagaatat acccagctgg tgctttgact tctttgacat ccaatttatt ttgaacatat
     3301 tttccgtctt tttcatagaa tgcagggatt tcttttaagg cgatttcccc tttggcatcc
     3361 gttgtatatt gttttgtttc tcccgtatca tgacgtttga tttcatacgt cacatttgga
     3421 acttttttcc ctgtgacagc gtctagtttg gttagtttga agtcgccatt ttttagaact
     3481 ttaatagtta gtccagctct attaggatct ggcacataaa gctttcctag attttgagca
     3541 acaggatcta cataggcaaa acttgaacct acccctctag aatttggatc cagtgaagaa
     3601 acagaaattc gtccgtcatt tgagttttga tttgctttta taaacaaact atttcctgat
     3661 tttcttactt gaacgttagc tgtgttttca acaaccgacc catcccattg agaaaacaca
     3721 ccttttgtat ccttaacttc tacttcttcc cctactttaa ccgtcactgt ttggctatca
     3781 aaagaaggat cttcaagtaa attgtttatt tttctatcta cagtctgttt gaaagcttca
     3841 taattagatt gtccaatagt tccataatta acaaatgaac cacctaaaac ttcccaaata
     3901 tatgtttgtg ctaatacata gttatccatt gaaccactag aatagtagcc aaaataacca
     3961 attaaagaaa tcttttttct aatagcactt gtcgtaatta aattttcgag tggtgtcaca
     4021 tcatattgaa tacccgcact cgtgattgcg ccttcttgaa tgcaaaaaac atcttctccg
     4081 tcaatttgcc agcgaaccat gtaatcatga gtatacattg ttccttcagg agtaattcga
     4141 gtcatattca catctttacg accattttca gtcaatgtta gattggtagc acgtgtctgt
     4201 gcattctttt ccaagattgc ttgccattct tcagattctt gcgccaatcg ttcttgttca
     4261 gaaaaagaag tcgctttttg aatatcttca atggttataa agtatttctc ataaccttca
     4321 gggacctctt gagtcattac attgggcgga gaaacatctt ctgtcgtgtc ctgcgtagtt
     4381 gtctcttctg ttgtttcact tgtggtttca gtactgatcg cttcttcact actttttgat
     4441 tcttcagtct tcattacttc ttcagtagaa tttgattcat ttatcgtttc tgcaaatgcc
     4501 gttacagatc ctaacatggg attgagaaat gtaacgaaaa ttgccaacca agttgccatt
     4561 cttttaaaca tatttttttc gttcctttca ttttattttt cagtcttcta aagtaatttc
     4621 ttatggactt cgacaccgaa acatttttat cctcctcaaa acaaaaaaag actagaactt
     4681 ataattctag tcttcatcaa ccacttactt ttcttttatt tttagtagaa atctacagtg
     4741 tgtttttgtg acccgtcact gtaaataata acatccacca taaaaccttt gttgtaccac
     4801 tgcgaatttg gatcgctaac ctctgcatta gcccaatcat aggcttcatc tacagtgtta
     4861 aatacctttc cactattacc aatctctcca actacaattg ttggctcttc aggtgtcgtt
     4921 gtgccaccat ttccgccttg atcgcctcca ccaggattgc ttggttcttc aggcgttgtt
     4981 gttcctccac ctgtgcttgg gttagaagga gttgtttctc ctccagtatt tccaccatta
     5041 cctggttgtg taggtgtttg agtagtgcca ccattgtttt ctggtgtact tggcgttgtt
     5101 gtaccaccag ttgttcctgt ttgatctcca gctggtgttg ttggtgtcgt tgtatttggt
     5161 gtcgtttgtg tatttgaagt cgttccttgt cctactggtt gattcggatc aactttgtct
     5221 ttctcttgaa tcactgtttg agccacagtt tcacctgcgc tgttttgtac acttgctaca
     5281 ttgccattaa atactaattt atttcctgca tagattaaat ccacatttgt aatcttattc
     5341 atgtccgcta atttttggac agtaatattt gttgcttgac tgattgcgct tagggtatct
     5401 ccccagacaa ttgtgtattc attcccttta atttctgctt tgatttgttc cgccgaacga
     5461 ggtgtccaaa ctttgttttc tgctgcttca gcattcgttc ccacagcaag actaactcct
     5521 ccgactacga ttcctagtgc caatactttc aacattttca ttgtttttcg cctccgattt
     5581 gaataaattt atttacaaaa acatagtacc aaacttatgt ctatcagtcg atatattttt
     5641 tgtagatttt tttatttttt gatatactaa ctttggtact aaaataacta aaaggaatgt
     5701 cacacatgca acttaataat ttaaatgaaa tacatgattg ggctcaaaat aatttactta
     5761 atcgtaaaca agctgccaaa atcactggac aaaactatga tgctttttct agctctttac
     5821 gtagaaataa tatcaaacca tttctagaaa taggcgacga acctggtcct tccatcattc
     5881 gcttatatct aagagaagat attgaaaaat acgcaaaaca gctcgaagaa aaaagacaga
     5941 aacgtcaatg aggcgttttc tgtcttggat ttacaagtaa aacaataata aacactttta
     6001 caacggaggt tctatgaata agaaatacat tgtttcagtt ggttcaatcg tactgattgt
     6061 actcgctatt attgcttttt ctgtatccaa caaaacagaa acacagccac aaaatacggt
     6121 tcaatcgact actacaagtc aatctagcga tcattccgtc tatccttcac taaatagttt
     6181 aaaagaaacg catggacaag atttaattga tcttggttct tctcaaatta ctactgtctt
     6241 atttgaaaag aaaaatattg cgtttattac tcctaaagaa ggaactgacc ttgaattttc
     6301 tatcaaagta acaaatacca aagaaaattt attctccatt cctgacattc aatatacccc
     6361 taacaagtta aagattgggg atacgtttgg tttggctaaa gtaaatgaac aatactttgc
     6421 ttactcactg aaataagttt ctaatagcaa gaggaaaaac cctcttgtta tttttatcac
     6481 ataatcattt tataaaaacg cttcgaaaat atcaacattt ttgaagaaac tatttttcta
     6541 atttctcaat ttcttccaag ggattttcgc taatgaccca tacaggcaca gattgctctc
     6601 gaggctcccc ttcttctaac caccaacctg cataatgaac ttcttcctcc tcagcattac
     6661 catgatacca accaacaaag gattcatcta caacgaatga aatttcttct tctggatcaa
     6721 ataattcatt tttccatgct tcaattgctt gttctttggt gtacttttgt ttgttaaata
     6781 caccacaaat ttcgtcatct acataaaaaa tttcaaaatc aaaattactc gttcctttta
     6841 ctggcatctt aacttttcct cctttgactc acttcaatag atataccact catgttcatt
     6901 ccattgttta ctagttaatt ggagttttat cttagggctt tgtgcactct ttccaaaaac
     6961 ttgagcgtcg tttttttctt ctaaccaaaa aaggtaattt cttacattcg gtactaaatg
     7021 tttttcctgc atttcttcat gaaaaacata taattctgct tcatttttca accaattcat
     7081 tattcattcc ctcattcact tcttcatacc gccattcaga tagtgagatt tctttttgtt
     7141 tctccacttg tcctataaaa atcgctgtat tcaacaagct gatcacactt aataataaag
     7201 caatgaaact aatggttaca cttactcttt cacattttgt cattttgttt ccttctttac
     7261 tcgtttgtta tttcttgtaa taggtctaat aactccttca ccgaaatttg caaatggttc
     7321 gcaaaaattt caataaaagc actcgcacac attaccagac cttcaactga tatatcactg
     7381 cgacaataaa attctggaga aacactgatt tctgattctt tcttacaatt gaacgaaaaa
     7441 actatttttg aatcaaattc cggtaattct tccacaacta tgttttggga ttctaccaag
     7501 aaagggagtg cctgttctaa tgcttcttct ggtgtttgag cagatagaat aattgcttct
     7561 tgttctttta tttcattgtt ctcaaatact ttataacttt ttttcattgt tagttcctct
     7621 ttcattcctc tctcaattag ctaagccgtt actggtacgc cattctctaa atttagtatc
     7681 gctaatcctt aatgctcgtc gaatatcaat cattcgataa cctattgcac gaagttcttt
     7741 atatctttta gcagttagcg tctctaacgt catttcaggc aatgggttcg aatcaaaaat
     7801 agaattcgaa ccgattattt gggccaattg ttgtacttgt aacagctctt ctgaacttaa
     7861 ttcagtgatt gctcgattgc ctatcattgc atacattttc tgtcgaatat acttcttttc
     7921 ttgtttagac aacatttcta tccactccta ttttcattat catttgttac ctccacttca
     7981 gtaaatccac aaataaatga attcatattc caagtaccat tcccttcaac tacaactttt
     8041 ctcctgtcct ctttcggata ttcaagaatc agaccgttta ccaatatgtt tttcactact
     8101 actaatttgt ttgtataccg tggaattgct gttccgatat acttcacttt atttcctacg
     8161 ctaattgtca tttcctcact cctattcttt tttatccaaa actttttcct gtcctattcc
     8221 tgccaacgaa tatacttcac tgcatgctta aacaaaacaa aatgttgccc tgttttattt
     8281 ccgtctttta cttgttccac atacagttca tatttggtca caccttttaa aacgccagat
     8341 actttttttc atccattgtc acaattagtt ttttcatcaa tcgattgata ctgcactgct
     8401 catttttctt gttcatatag tattcctttc tttttcaaaa ctagcctctt ttgtttacta
     8461 gttttcatca atcatcaacg atttaacaga ttttttcttt cgcagggttt ctagcgtcag
     8521 caaccccaaa ctctaaaaaa gtgcctttgc tcccttgaaa aacgagcgga cgattgtact
     8581 cgtcatcata tgtttttcat actacctgat ccacgcaaat aacctttttt tgttctgtgc
     8641 cacacccatc catacgtatc gcttgagatt caccatactt ttttaggtgg tttatccttc
     8701 ctaagtccct gttagacacg ctgattaact tagaaagtcc tgtttgtatc caattttcaa
     8761 agatctccca aaagaaataa gtaggtttac gtgtatcgct tctgttcaaa gtacctgttt
     8821 gtacttgagt atttctactc ccctcgttca agggattcct tccaccagct agcttttcct
     8881 ttcatcatca aatgttcctg aaaagcattt acgtttcgtg tccgtgtgct tacttattct
     8941 tttttcgata gcgattatcc gctcgctact cgctatttta tactttgttt gttagctcaa
     9001 ataaaaacta acaaacaaaa aaggctagtt tctttccgtt ttattttttt aactgtcgat
     9061 aaatagttgt tagtttaatt ccaagttcta aaaccaatac gcccaacaaa accacaggaa
     9121 aaacaattgg ggcaaacaag taaaatcgga tagttttcat gtcttccctc tttctttttg
     9181 gtgtcgctgt ggtcgttctt gccgttcaat acttcgatac attttttatt cattatttgc
     9241 tcgttttgtt ttcctctgga ctgcttttct tgctttttga atactttata cgcttttcgt
     9301 gcctgaatta ataggtcttg tcggcgtttc attcgtgaga aaacaatatt tgaaaccatc
     9361 aaatcctggt tcgaacaacc acgattcgca ataattttcc catcaaaaaa aatagttttc
     9421 acttgagaaa aaaacagttg gaaatcttgt tttgttgcta atttttttgt caaaatcact
     9481 gtgcttttcc ctgttgaatc tacaatgatt cttcgaacaa tccgattcga taacttccaa
     9541 cgttgccgat taaacaatgt tacttcacaa caaatatgac gtaagtgatt aggggtcatt
     9601 ttcatgtgtc caattcctcc aaccaattat gcatcgaaat tttgggcaat tgtgacagcc
     9661 cataaatctg cgtttccagg tcttcataac tacaagaacg cttggcttga agtaatgaat
     9721 attctgcttg atctgatagt tttgttgcca ttggatctag attcaattct cttacacaac
     9781 attcccaaaa attcaatagc gctctaaaat aaaatccttt tcgattctga attgcttttc
     9841 cttcatgacg agcaattttt tcattttgaa tacaccgttg atacgttttg cttaaatctt
     9901 ctgcaaatgt ttcccctaaa attcgatact cgcttccatc agataaacca tgtgtttgat
     9961 tatactttcg ttgccgcttt tctataattc gtttactgtc gtatatcatg tttagctgct
    10021 ctttcgcttc acgccattca gctgaactgc gaacaatcca tgtcaaaaca tctttcggaa
    10081 caaacgtact ttcgctgtaa tgagcaataa actctttttg ccaatcatca gccatttcaa
    10141 caggactagt cgtatcgatt tttgtatcta gattttctgt atctagattg ttagaagaat
    10201 tattaaataa ttgtgcagaa ttttcacatg gatttttttc gtcaaaatca tcgaaaatcg
    10261 ctaaattatc aacagattcc tcctcttctt ttccttgtgc agaatctaca ctggttgagt
    10321 caacaggacc taatgaatac acatcatttt ctgtcgtatc taattctcct agatacaacc
    10381 gagctttgtt tctttttcct gtttgttgat caaatcccat atcctctatt tctagtagct
    10441 catattcttt caattctttt ttgattgtat taacagaacg tctgcaacaa ttcaatgcat
    10501 tggctaattc ttgttccgta aattcaaaat aaacaaaacc ttgactatct acttgatttc
    10561 gttgaatggc taaatctaag cgtgaccgaa aaaccatata cgcgattttt gctttatcag
    10621 acaatgagcg atatttttca cttgtaataa atacctttgg aaattgatag tataattcat
    10681 tgtatttacg agttgcggta aatctttttg ttgccatcgt tattacacct catcttgtcc
    10741 atatattaag aaagaatttt ggtgttggta gacacgaaca tcttccattg tagcaatcaa
    10801 ttcttttaga taaattcgac cgaattcaat tgttagtaag tcgtaatctt ctaattcatt
    10861 tttgatttct tcaatttctt cttcagacac attgagtaaa atggctaatt cttgattggt
    10921 ataagaaata tagacatgtt tcttttcatc aatccgtttt tcaattaagg attcttctaa
    10981 gtactcattc agtaagctat aggcgtattt tgcttgtgca gtcatcgcat aatattttga
    11041 cgattttata aagaatagtt ttggcatatg aaaagttgct tgtgtcagca catctgttgc
    11101 gcgaaatttg ttttttacca tattttttcc tcctagaaaa acacgcaaaa aaagctctgt
    11161 ataaatcgaa ttacacagag tttcttcttg ttgatattta atttctaaaa tacgtttcgg
    11221 gtaaaaacta aacacttact aatatttaga ttgtagaatc t
//
LOCUS       DACDPS010000074        11028 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.074, whole genome shotgun sequence.
ACCESSION   DACDPS010000074 DACDPS010000000
VERSION     DACDPS010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.074"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(53..988)
                     /gene="murB"
                     /locus_tag="IWJ07_002133"
     CDS             complement(53..988)
                     /gene="murB"
                     /locus_tag="IWJ07_002133"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAP9333701.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1061..1981)
                     /locus_tag="IWJ07_002134"
     CDS             complement(1061..1981)
                     /locus_tag="IWJ07_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9333702.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2094..2846
                     /gene="xth"
                     /locus_tag="IWJ07_002135"
     CDS             2094..2846
                     /gene="xth"
                     /locus_tag="IWJ07_002135"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAP9333703.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            3833..4366
                     /locus_tag="IWJ07_002136"
     CDS             3833..4366
                     /locus_tag="IWJ07_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAP9333704.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(4856..5380)
                     /locus_tag="IWJ07_002137"
     CDS             complement(4856..5380)
                     /locus_tag="IWJ07_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9333705.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(5373..5846)
                     /gene="tsaE"
                     /locus_tag="IWJ07_002138"
     CDS             complement(5373..5846)
                     /gene="tsaE"
                     /locus_tag="IWJ07_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAP9333706.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(5969..6949)
                     /gene="pta"
                     /locus_tag="IWJ07_002139"
     CDS             complement(5969..6949)
                     /gene="pta"
                     /locus_tag="IWJ07_002139"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAP9333707.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(7102..7782)
                     /locus_tag="IWJ07_002140"
     CDS             complement(7102..7782)
                     /locus_tag="IWJ07_002140"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP9333708.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            7908..8777
                     /locus_tag="IWJ07_002141"
     CDS             7908..8777
                     /locus_tag="IWJ07_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9333709.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            8803..9318
                     /locus_tag="IWJ07_002142"
     CDS             8803..9318
                     /locus_tag="IWJ07_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333710.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(9365..9928)
                     /locus_tag="IWJ07_002143"
     CDS             complement(9365..9928)
                     /locus_tag="IWJ07_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9333711.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(9983..10162)
                     /locus_tag="IWJ07_002144"
     CDS             complement(9983..10162)
                     /locus_tag="IWJ07_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333712.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(10239..10748)
                     /locus_tag="IWJ07_002145"
     CDS             complement(10239..10748)
                     /locus_tag="IWJ07_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333713.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(10844..>11028)
                     /locus_tag="IWJ07_002146"
     CDS             complement(10844..>11028)
                     /locus_tag="IWJ07_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP9333714.1"
                     /translation="NYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIREDTFTGKK
                     MTFFFDPDGLPLELHE"
BASE COUNT         3155 a         2265 c         1971 g         3637 t
ORIGIN      
        1 agcaggacgg ccaccgtcct gctcttttac ttattttaag ttccattact tattaattgg
       61 ctaaacttct gcttgtttct gatattgctt gtttttctcc aatgatccgc acttctgttt
      121 ctaaagacac atcaaatttt tctttgatta cttcttggat gtgcgcaatc aattcaatat
      181 aatctgtcgc tgtcgcatga tcgatattga cgatgaatcc agcatgtttt tcagagactt
      241 gtgcaccgcc ccatttcagg ccttgcaatc ctgcttgttg gatcagctga cctgtatagt
      301 gtcctactgg gcgcttaaag acacttccgc atgaagggta ttctaatggc tgttttgatt
      361 gacgcaattc ggtcaactca tccattcttg ctttgattgc ttcataatcc cctgtttgca
      421 aactgaatcg agcttccaat acgatagcac gtaattcttg gactttactg tgacgataag
      481 aaaataccat ttcgtctttt gtcaaagttt tcaatgttcc atcttcaagc aaaagatcca
      541 cttcagcaaa aacatctttg atctctccgc catacgcacc tgcattcata taaaccgctc
      601 caccgacact tcccgggatg ccgcaagcaa attcaaatcc agtcaatgat tctgccaaag
      661 ctgcataagt cgtatcaatc agtgaggcac ctgcttccgc aatgaccatc gttcctttga
      721 catgaatctg tttcatttca gtcagcatga tgactacacc gcgaatcccg ccatcttgaa
      781 cgatcagatt gcttgcattt cctaatacca tccaaggtat atcatggatt cggcaataat
      841 cgatgatttg ctttacttct tcttgttttt tcgggaatgc aaggacatca gctggtccac
      901 ctgttttcgt aaacgtatag ttcatcaatg gttcatcaaa caatatagta atcgtggcta
      961 agctttcaac tatttcttct ttattcactg aggattttcc tttcttttct caaacgtcct
     1021 cattattcta tcacgcttaa tacaatcttg ccagtcttgt tcaaagttga tgacggtagt
     1081 gatctttaag cacttcctca cataattcat gacttcttac aacttctttt tgtttaagca
     1141 attctgtttc cgatttacct aaacggatct gagaaaagaa ggcctctacc attggctcaa
     1201 acccgcgttt gactagcgtg tttgtccagt cgccaaattc tttcactttt tttccatcag
     1261 catcttgaat gacaagctgg gtcagatcgt ttacttcata agtaccattt ttacttgtta
     1321 cttgatattt ttctatatta gcgccacttg ctaaatccat cgttaaaata gccgtttgat
     1381 tttgtgtttc taattgtaaa atggcatatt ccaaaaattc ctttgtttct cttatctggt
     1441 attttgttct caaaatgggt tcatctaata aatacacagc tgtatcaata agatgtaaaa
     1501 acaaatcata aaggacgaac tctgtcgttt cttttgccgc tattcgattt ttttgcaggt
     1561 ggatcaaccg cttatctggg atacgtttca attcttctac cataggagca aagcgccggt
     1621 tgaaaccgat catcaaaatt atttggttct tatcagctaa ttcttgcaat tcacgaactt
     1681 catttagatg gacactcagt ggtttgtcaa tataaacatg gataccattt tctagacatt
     1741 ttttggctaa ttcataatgt acttttgttg ctgcgtggat catacatgct tcgatttttt
     1801 cttctaaaag ctcatcgatc gtttcatata gatgttggaa cccgtactct ttttgaattc
     1861 tttcttttgt ttctttattt cttgttgcaa aataaaaatc aagtgttcct tgttttttcg
     1921 cataagttgg taaatatgct ttttctgcga tattccctac accgattaca ccaatcttca
     1981 tctactttcc tcctggatca tttagttact ttcatcttac accatttttt actaaaatga
     2041 ggtacaatag tatcgcaaag gtaaggaaat taattcgaag gagtgtgctt ttattgaaat
     2101 ttatctcttg gaacgtaaac gggcttcgtg ccatcgtaaa taaaaatttt ttagaagttt
     2161 ttcatgagtt agacgccgat tttttctgtc tgcaggaaac gaaactccaa gcaggacaaa
     2221 ttgatttaga tcttccaggt tattatcaat attggaatta cgcggaacga aaaggctatt
     2281 ccggcacagc gatttttgct aaaaaaccag cattgaatgc cacttacgga atgggtatcg
     2341 atatccatga tacagaaggc cgtctgatta ctttggaata ttccgatttc tttcttgtaa
     2401 cctgttacac gccaaattct caaagcgaat taaaacgttt agattaccga ttagaatggg
     2461 aagaagcctt ttataattat ttggaaaatc taaaaaaaca aaaaccagtc atcgtgtgtg
     2521 gggatctaaa tgtcgctcat caaaagattg atttgaaaaa ctggaaaacg aatcaaaaaa
     2581 atgctggatt cactccagaa gaaagagcgg ctctttctcg actgttggat aacgggttta
     2641 tcgatacatt ccgttatttt tatccaaccc aggaaggtgt ctactcttgg tggaactacc
     2701 gattcaattc aaggaaaaac aatgctggtt ggcggatcga ttactttttg acgagcaaag
     2761 atttagagcc aggactagct gatgcaaaga tccatacgga cattatgggc agtgatcatt
     2821 gtccagtaga attagatttg aaataaagag gttgtgaaaa agtcgtttag tttcgagtat
     2881 taagaaaaat tttgtgaaaa tggctttcca cattttgcat aaatttggca taataccgag
     2941 aaactggctt ttgaacactg tttatcgaga gattgtgaaa aagctgtcct gcatcaagta
     3001 ataagaacag ccaaacaaaa atagttcctc atatttttcg tttggttggg cttattaccg
     3061 cagatacaag cttttgaaca ctgtttatcg agagattgtg aaaaagctgt cctgcatcaa
     3121 gtaataagaa cagccaaaca aaaatagttc ctcatatttt tcgtttggtt gggcttatta
     3181 ccgcagatac aagcttttga acaccgttta ttaagaggtt gtgaaaaagc tgcactgcat
     3241 caagtaataa gaacagccaa acaaaaatag ttcctcatat ttttcgtttg gttgggctta
     3301 ttaccgcaga tgcaagcttt tgaacaccgt ttattaagag gttgtgaaaa agctgcactg
     3361 catcaagtaa taagaacagc caaacaaaaa tagttcctca tatttttcgt ttggttggac
     3421 ttattaccgc agatgcaagc ttttgaacac cgtttattga gaagttgtga aatcagcgtc
     3481 ctgacctgag tattagagaa acaaatggca aaatagttcc tcatattttg ctattttttg
     3541 agctaatacc gagggtcagc tgattgaaca ccgtttagtg gagaagttgt gaaaaagtcg
     3601 cctactttcg agtcgtaaga aatttttttg aaaatggttc ctcacaactt tgcataaatt
     3661 tgacttaata ccgagaagtc atgatcccca tctaactcca aatcacaagg aatgagttca
     3721 aaaactagct tttctcaaca aatattacat aaaaaattct atgttttttg ttaaatttat
     3781 ttgctttttt caaaagtatg ttacagtaat ttcccaagga ggttttacag aaatgaattt
     3841 tgttgccatg gattttgaaa ctgccaatca tcagccttat agtgcttgtt ctcttgcgct
     3901 agtcatggta aaaaatagcc agatcgttga tgaattctat acattgatac agcctgagac
     3961 tccttttttc tggcgaaatg tccaaattca cggaatccat caagaagacg tacgcaatgc
     4021 gccgaaattc ccagaagtct ggcaaactat ccaaccatat tttcaaaaaa atcgtttagt
     4081 cgttgcacat aatgctgctt ttgatacaaa ggtcttagcg ggatgcttag attattatca
     4141 tcttgaacag cctaattatc tttctttatg tacagtgaaa agtagccgtc gcctgtttcc
     4201 tgaatttcca aatcatcgat tgaacactgt gtgcgaaaat ttgaacatcc aattagatca
     4261 tcaccatgat gcattggaag atagtcgtgc atgtgcggaa atcctgcttt atcaagaaaa
     4321 aaaattcggg acagatccat tgaaaaaact tgtgactata aagtaaaaga atacttatga
     4381 aacacacaaa gaggtcgtga gcctgccgtt tcactccgag tcacaaggag caatttcaaa
     4441 aaatagcttc tcatgtttat taaatattgt gactggtgac cgaggagctg gcaggagaac
     4501 accgtttatt ggagaggttg tgaaaaagct gttctgcatc aagtaataag aacagccaaa
     4561 caaaaatagc ttctcatatt tttcatttgg ttggacttat taccgcagat gcaagctttt
     4621 ggacaccgtt taccaagagg ttgtgaaatc agcgttttga cctgagtatt agagaaacaa
     4681 acggcaaaat agttcctcat attttgccgt tttttgagct aataccgaag gtcagctgat
     4741 tgaacaccgt ttattcaaaa aaaatggact gcgacacgac ttttgtcaca gtcccttcgt
     4801 gtgtttttct gccttctcaa aaggatgaca gtttctcact cttttcctat ttcttctatt
     4861 caatcaaata actcatttta tatacatcta aaaattcacc tagatctgtt cttgcccctc
     4921 taggcatgac cgcttcgatt tggaagccca tttttcggta aaggtgtacg gctctttcat
     4981 tacgaacttg aacgtcaagc tccaatcgaa aaagtacacc catttccttc gcccaactga
     5041 tgatttcttc tagcataaga gtgcctagtc ccattcccca atattcctgc agaatactga
     5101 taccgacttc tcccacgtga gacaaacgaa attgactgtc tgccttgaca gatgctgttc
     5161 caataatcgt cccttcatag attgcaagca gcaataaatt attattgctt tcgtatatat
     5221 aatccaactc ttcttctaag gtttctggtg gtaacaggag ttctgcttca tccaaaacta
     5281 aaaattctgt ctccttatta acttgtccca tcatcaaaag aatgtctttt gcatctcctg
     5341 gcactgcttc ccgcaagatg atcccaactt cttcactcat gattttcagc ccatttttct
     5401 agaattcgct gtttgaaggt ttcactttgc tccccgtatg agattagttt cacataccgc
     5461 ttttctaaat ctgtatcact tttttctaaa atcagttcca aataatcctc cggcagtgct
     5521 tcttctaata gatttcccca ttcaacaacg gaaagcccat caccttcaaa atattcatct
     5581 aaccctaaat ctgctccgct agctgctacc cgataaatat ccatatgata caacggaatc
     5641 cgaccttgac tatattctct aataatcgtg tatgtcggac ttttgatcat ttgcgttatc
     5701 cctaatcctt gggcaattcc tttcgtcaac gttgttttac ccgcacctag atcaccagtc
     5761 aatatcagat tgtcacctgg cattgcggaa tgaccaattg cctctgcgaa ttgttctgtc
     5821 attgttaatc ctgatagttc gatcattttt catcttctcc cgctcttctt tctttttgcc
     5881 agtatatcgc gtaatgaagt ttttttaaag aaaaaatgaa ctagtcaaga acagcagctg
     5941 ctgtttttgg ctagttcgcg tgtatttgtt aatcaatcaa tgtttgtgct gctgtgataa
     6001 tcgataattt atatacatct tcttcatttg ctccacgaga caaatcagaa accggtttgt
     6061 tcaagccttg tagaattggt ccgattgctt caaagttacc gaaacgctga gcaattttat
     6121 agccgatatt tcctgactgc aattctggaa atacaaagac tgttgcttga ccagctactt
     6181 ctgaatctgg cgctttcagt tgtgcaactg aaggaacata agaagcatca aattgcaatt
     6241 cgccgtcgat catgtattgt ggcgctagtt ttttagcgat tttagttgct tcaactactt
     6301 tgtctacttc tggcgatttc gctgagcctt ttgtcgagaa gctcatcatt gctacttttg
     6361 gttcgatatc gaacaattct gctgtttttg cagaatctac ggcgatttca gccaattctt
     6421 gcgcagttgg attcacgttg atcgcacaat ccgaaaagac atatttttct tgatctcttc
     6481 cacgtaccat gatcatcgct ccgcttgtcc gactgatgcc aggctttgtt ttgatgatct
     6541 gaagtgctgg gcgaacagtg tcgccagtcg aatggatcgc tccagagacc attccgtctg
     6601 caatccccat atacgtaagc atcgtaccaa agtaattcac atcttttaaa atcttttctg
     6661 cttgttcttt ggatgccttt ccatttctac gttcaacaaa agtatccacc atttcatccc
     6721 acttatcgta attatcaggg ctgatgatcg taaagctaga tgttttgatt cctctggcat
     6781 gcgctgcatt tcttatttct tcttcgttcc ctactaaaat gggttccaca agttcttctg
     6841 ctttcaaccg ggcagctgcg cctaaaatac gagcatcagt agcttccggg aaaacgattt
     6901 tgatcccacg gcgaacaatt ttaaatctta aactatcaaa caattccaca agatgacctc
     6961 cagacgtttt tatatgtatt atcatacact attccagaaa aaaagaacag tttattttat
     7021 ctgtatcagt caaaaatgct ggaaaaatta agtttttcac atacaaagcg aactgcacta
     7081 taacaggaaa gcgctgtttt attacactgt ttcaggcagt tgccaatcaa tcggttcttc
     7141 tcctaacgca aggagcgcat cattcgtttt cgagaagggt ttggaaccaa aaaagccacg
     7201 acttgcagat aacggactgg gatgtggaga tgttatgatg atatggcgac tcttatcgat
     7261 cattttgatt ttttcttgtg ccggttttcc ccacaagata aacacgattg gtttttcccg
     7321 ttcatttaat ttttcaatga tcgtgtctgt taatctttcc caacctttcc cgcgatgaga
     7381 atacgcttgc ccttcccgaa cggtaagaac ggtgttgagt aaaaggactc cctgtttagc
     7441 ccatgacact agatttccat gttgaacagg tgcgattcca agatcatcat aaagctcttt
     7501 atagatattt cttaacgatg gcgggatctt tacgccaggt tgcacagaaa aagataagcc
     7561 atgagcttga ttttctccat gataaggatc ttgtcctaaa atcaccactt taacttcttc
     7621 ataaggagtc aattccaatg cttcatagat atgatacata tctggatgga tcttttggga
     7681 cgcatattct ttttttaaaa attctcttag atgcagataa taatcttttg aaaattctga
     7741 ggacaaaact tcttgccaac tgttatgaat gatttctttc aaaaactcca cctcctaata
     7801 aaaagaataa ctcacatttt cttcaatcac aagggacaat cttcaccctc agcacttgat
     7861 taatatggta aactaggaac aagaagcaga aaggaacgat aatcattatg atcaaattaa
     7921 ttgcatcaga tatggacggc acattattag atgcccatat gagtgtttct cctgaaaatg
     7981 ccgaggcgat acgttttgca aatgatgccg gtgttgagtt catggtcgct actggtcgaa
     8041 actatcaaga agctcgcgca gctttagatg aggtaggaat cgattgtgcg atgatcactt
     8101 tgaacggggc acaggtattt gataaagaag gaaattcatt gtttaccgtg tcgattccaa
     8161 ataatcaagc aatctctgtt ttagatattc tagatgctaa cggtatttac tatgaagtag
     8221 caaccaacga tggtctttat tctgaaaatc aagcgaaacg aattgaaagt tttgcttcca
     8281 tggtggctac ccatttacct caccttacgt ataaaatggc gattgccatg gcctcagcta
     8341 agctggagtt attgcacatc acatacgtgg acagtattcg tgctattttg gaagaaaaga
     8401 atctggaagt tttaaaaatt atttgttttc atacagaagg gccagctgta ttaggtccag
     8461 ttggaaagca gatttctctt ttaggcgatc tagccgttac ttcctctggt caaaacaata
     8521 tcgaagtgaa ccataaaaac gctcaaaaag gaattgctgt tgcccatgtc gctcacgagc
     8581 gtgggatttc tttagaagaa gtcatgacga ttggcgataa cttcaatgat gtcagcatgc
     8641 tgcaagtagc aggagtcagt ttcgcaatgg gaaatgcaga aatcgaagta aaagattatg
     8701 ccaaatatac aacggacaca aaccttgaat ctggcgtagg aaaagccata ttgcgcgcga
     8761 ttgcagaaga cttgtagttt tatctagaaa agagggatat agatgtcaga atttttcatt
     8821 caggaaaagc agcttggacg tgcaactcgg acaattgtca aaaacgaaaa aggacattcc
     8881 ctgtttttga tggttggtcg ctggggaaca aagggcgatg cattgtctct ttatgccatg
     8941 aatggcgatc tcgttgctca catcaagcag ataagcctga cattcggtac gcgttttgag
     9001 ctgtataaag agttcgaaaa ggtgggaacc atgcagaaaa tattcaattg gcccggtgac
     9061 ttttattata ttcgtagact tcactggaca gtccaaggtg atatctataa tcatcaatat
     9121 caaatccatc atttcaatca gcaaatcatg aaaatggata aagcaacact tttttcagga
     9181 gattactatg tattggatgt ctcaaatgat gaagatgctc cagtttgtat ttgtattgct
     9241 gcggttttag attactggct ctaccgtaaa gacaaaagtc gcgataagcc tgtttcttac
     9301 cgacttaact taagttaaaa agatgtacct gaaatgcgga aatacgcatt tcaggtacat
     9361 ctttttagat attcttttgc atatgatcat aaagatgacc actgatcatc cgctgaccga
     9421 caactttgaa gtcatggcgt tcgtataatt ttttagcctt tggatttttt tcatccacac
     9481 ttagaccgat gacggagcga ttagctttct tcgccaattt aggtaatgct tctaataatc
     9541 gcgaaccgat gccttgtcct ctaaaatctt ctgatacact gatagagtcc aaataccatt
     9601 cgttgggaaa cgtttcttta tcaatgaaca aacgtgtaga cgcatctagc tgatatttct
     9661 ctaagatagg taccagtggc tgatcgatga ttgattcttc ttcatctgta taaccgtaag
     9721 caactcctgc aactttaccg tctatttctt ctacgatacc tcgtgaataa ctgtaacgat
     9781 aatttggatc agtgattgct tcttctaaaa tctctaaagt cttttgttcc cctgtctgct
     9841 tcaaaaatgg cagttccata tcttttagaa tcactaagat caatggggcg atttctggag
     9901 catcttcttt tctcgcaaaa cgaatcatag tttcctctcc ttttctccat tctaacatgc
     9961 agaaaaagaa atcgcaaaat cgttatttta aaaaatcttc aatcttttca gtaatgaaat
    10021 aaccatgagt aacgattctt gccgcttcag ttcggcaata agggcagacg actggcattt
    10081 ctttatcatt atgttcgact acattattga cttcttcgta tgcaaatttt tttccgcaat
    10141 tagggcattt cattttttcc atgttatctc aacttccttt catctttact ctcttagctt
    10201 actcttatat tgtaaaaaag agaagcaata tccttcttct atgcagatat ccatacctga
    10261 cggtcctgtt ctccatctat taatcggact ttataataag gacgttcatc tttgactaga
    10321 agtgtccact ctttcacttt tgcttttttc ccgtagtagc tgactgcttc cttcatcgct
    10381 gcttgcggtt gtttgaactc ttccgttttt tctatatcaa aaagatgttc tgcggccttt
    10441 tccgtgtcat ttcgtacggt tgttttccca gtggatggat tgactgctat gacttgtcct
    10501 tcacaaaaaa actggtattc atatgttgca ttcgtaggat tctccaaatc tttttcaact
    10561 gcaaaatgaa tagcagatat gttatgttct tttgtttcgt tttttacgat tgctgctgct
    10621 tttttcaatg agatccgata ttcctggtca ctaatatcat ccatgtgaaa agcggcattg
    10681 agcagtcccg tcataccggt aatccctatt acgatgaggg cagcaacacc aaacatcatt
    10741 tttttcattc ttcattctcc tgtttctttt ttttaaagaa tatcaagaaa tgaacgaatg
    10801 aaaaatcgaa tgaaacgaaa aaaaagtgac aattttgtta tctttattcg tgcagttcta
    10861 atggcaatcc gtctggatca aagaaaaacg tcattttttt tcctgtaaag gtatcttctc
    10921 taatcggttc tgattcgatc cctaaggact gcaattctgc aattgtttct tcaatatcgt
    10981 ctactttgaa agcaagatga cgcaagccac aagcttctgg atagtttg
//
LOCUS       DACDPS010000075        11025 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.075, whole genome shotgun sequence.
ACCESSION   DACDPS010000075 DACDPS010000000
VERSION     DACDPS010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.075"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            12..84
                     /locus_tag="IWJ07_002147"
     tRNA            12..84
                     /locus_tag="IWJ07_002147"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Val,seq:tac)
     gene            88..160
                     /locus_tag="IWJ07_002148"
     tRNA            88..160
                     /locus_tag="IWJ07_002148"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Lys,seq:ttt)
     gene            168..249
                     /locus_tag="IWJ07_002149"
     tRNA            168..249
                     /locus_tag="IWJ07_002149"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Leu,seq:tag)
     gene            267..339
                     /locus_tag="IWJ07_002150"
     tRNA            267..339
                     /locus_tag="IWJ07_002150"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Thr,seq:tgt)
     gene            351..422
                     /locus_tag="IWJ07_002151"
     tRNA            351..422
                     /locus_tag="IWJ07_002151"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Gly,seq:gcc)
     gene            439..524
                     /locus_tag="IWJ07_002152"
     tRNA            439..524
                     /locus_tag="IWJ07_002152"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Leu,seq:taa)
     gene            535..608
                     /locus_tag="IWJ07_002153"
     tRNA            535..608
                     /locus_tag="IWJ07_002153"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:569..571,aa:Arg,seq:acg)
     gene            618..691
                     /locus_tag="IWJ07_002154"
     tRNA            618..691
                     /locus_tag="IWJ07_002154"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Pro,seq:tgg)
     gene            711..783
                     /locus_tag="IWJ07_002155"
     tRNA            711..783
                     /locus_tag="IWJ07_002155"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Ala,seq:tgc)
     gene            811..884
                     /locus_tag="IWJ07_002156"
     tRNA            811..884
                     /locus_tag="IWJ07_002156"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Met,seq:cat)
     gene            898..971
                     /locus_tag="IWJ07_002157"
     tRNA            898..971
                     /locus_tag="IWJ07_002157"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="IWJ07_002158"
     tRNA            995..1084
                     /locus_tag="IWJ07_002158"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
     gene            1097..1170
                     /locus_tag="IWJ07_002159"
     tRNA            1097..1170
                     /locus_tag="IWJ07_002159"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Met,seq:cat)
     gene            1189..1264
                     /locus_tag="IWJ07_002160"
     tRNA            1189..1264
                     /locus_tag="IWJ07_002160"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Asp,seq:gtc)
     gene            1266..1336
                     /locus_tag="IWJ07_002161"
     tRNA            1266..1336
                     /locus_tag="IWJ07_002161"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1298..1300,aa:Gly,seq:tcc)
     gene            1350..1423
                     /locus_tag="IWJ07_002162"
     tRNA            1350..1423
                     /locus_tag="IWJ07_002162"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1384..1386,aa:Ile,seq:gat)
     gene            1448..1535
                     /locus_tag="IWJ07_002163"
     tRNA            1448..1535
                     /locus_tag="IWJ07_002163"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Ser,seq:gct)
     gene            1557..1628
                     /locus_tag="IWJ07_002164"
     tRNA            1557..1628
                     /locus_tag="IWJ07_002164"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Glu,seq:ttc)
     gene            1651..1740
                     /locus_tag="IWJ07_002165"
     tRNA            1651..1740
                     /locus_tag="IWJ07_002165"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Ser,seq:tga)
     gene            1753..1826
                     /locus_tag="IWJ07_002166"
     tRNA            1753..1826
                     /locus_tag="IWJ07_002166"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Met,seq:cat)
     gene            1845..1917
                     /locus_tag="IWJ07_002167"
     tRNA            1845..1917
                     /locus_tag="IWJ07_002167"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1878..1880,aa:Asp,seq:gtc)
     gene            1921..1993
                     /locus_tag="IWJ07_002168"
     tRNA            1921..1993
                     /locus_tag="IWJ07_002168"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1954..1956,aa:Phe,seq:gaa)
     gene            1998..2068
                     /locus_tag="IWJ07_002169"
     tRNA            1998..2068
                     /locus_tag="IWJ07_002169"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2030..2032,aa:Gly,seq:tcc)
     gene            2081..2154
                     /locus_tag="IWJ07_002170"
     tRNA            2081..2154
                     /locus_tag="IWJ07_002170"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Ile,seq:gat)
     gene            2166..2237
                     /locus_tag="IWJ07_002171"
     tRNA            2166..2237
                     /locus_tag="IWJ07_002171"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Glu,seq:ttc)
     gene            2503..3696
                     /locus_tag="IWJ07_002172"
     CDS             2503..3696
                     /locus_tag="IWJ07_002172"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAP9333715.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3826..5298
                     /locus_tag="IWJ07_002173"
     CDS             3826..5298
                     /locus_tag="IWJ07_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9333716.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5381..6169
                     /locus_tag="IWJ07_002174"
     CDS             5381..6169
                     /locus_tag="IWJ07_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAP9333717.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6236..6904)
                     /locus_tag="IWJ07_002175"
     CDS             complement(6236..6904)
                     /locus_tag="IWJ07_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP9333718.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7019..8731
                     /locus_tag="IWJ07_002176"
     CDS             7019..8731
                     /locus_tag="IWJ07_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9333719.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8734..10506
                     /locus_tag="IWJ07_002177"
     CDS             8734..10506
                     /locus_tag="IWJ07_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9333720.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(10588..>11025)
                     /locus_tag="IWJ07_002178"
     CDS             complement(10588..>11025)
                     /locus_tag="IWJ07_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9333721.1"
                     /translation="KITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEEN
                     KLIKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNLDKKVSSHMLRHSHISLLTEL
                     GIPLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT         3163 a         1978 c         2522 g         3362 t
ORIGIN      
        1 aagttatcca tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
       61 gcggttcgat cccgttaacc tccatatgag ccgttagctc agttggtaga gcatctgact
      121 tttaatcaga gggtcacagg ttcgagccct gtacggctca tatttttgcg ggtgtggcgg
      181 aattggcaga cgcactagat ttaggatcta gcgccttacg gcgtgggggt tcaagtccct
      241 tcacccgcat aatttaagtt ttacatgccg gcttagctca gttggtagag catctgattt
      301 gtaatcagag ggtcgagggt tcaagtcctt tagccggcat ctttaaacac gcggaaatag
      361 ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg ggttcgaacc ccgttttccg
      421 ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag acgcacagga cttaaaatcc
      481 tgcggtgagt gatcaccgta ccggttcgat tccggtcctc ggcatttcaa atatgcgccc
      541 atagctcaac tggatagagt gtttgactac ggatcaaaag gttaggggtt cgactcctct
      601 tgggcgcgtt aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa
      661 ggggtcgcag gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag
      721 ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct
      781 ccatttcatt taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc
      841 ggttcatacc cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga
      901 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
      961 tacaaggtcc attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag
     1021 ggaacggtct tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc
     1081 tcctttttca tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga
     1141 aggtcgtagg ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg
     1201 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
     1261 gccatgcggg tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg
     1321 attctcgtca cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg
     1381 cctgataagc gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat
     1441 tggttttggg gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg
     1501 aaaccggcgc gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc
     1561 cgttggtcaa gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt
     1621 acgggtcatt aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa
     1681 cggtcttgaa aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct
     1741 ttttcatatt atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt
     1801 cgtaggttca aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg
     1861 ggttaacatg cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt
     1921 ggctcggtag ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt
     1981 ctgtcccgcg ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt
     2041 gtcgcgagtt cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt
     2101 tagagcgcac gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt
     2161 ttattggccc gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc
     2221 gaatcccgta cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga
     2281 atgattttaa aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga
     2341 cagttctaat gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag
     2401 aaaactgttt tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg
     2461 gagatgcctt gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta
     2521 tttacatcag aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat
     2581 gcaatcttgg atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct
     2641 gtaacaactg gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt
     2701 caaaaagtcg tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt
     2761 gatggagata cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa
     2821 ggagttgatg aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt
     2881 ggtgctggtg accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg
     2941 cctttaccaa tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca
     3001 aatgaattaa cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat
     3061 caaggacaac cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta
     3121 gataatgaaa caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct
     3181 gaattattag atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt
     3241 ggtcctcaag gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt
     3301 tatgctcgtc atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct
     3361 gccagctatg ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa
     3421 gcagaagtac agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat
     3481 actttcggta ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc
     3541 gatcttcggc cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa
     3601 acagctgctt acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac
     3661 aaagtagatg cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc
     3721 caagtggtgg ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt
     3781 atttatctat ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca
     3841 aatgttactt tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt
     3901 acaatcgtaa caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg
     3961 aactgggtat tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa
     4021 cttgcggata aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc
     4081 ggttcgttat tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt
     4141 caagggatgg gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat
     4201 tctattgaaa agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt
     4261 gtttttggac cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt
     4321 tttatcaatg tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa
     4381 ccaaaaatca tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc
     4441 atgcttgtct cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt
     4501 gtttctgctt tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa
     4561 agagctaaag atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc
     4621 aacttggctg cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg
     4681 tggatgcaag gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta
     4741 tcactagtct ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact
     4801 cattgggtct tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt
     4861 atgcctttca gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc
     4921 ttaggtgtga ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag
     4981 cttggtgtag caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt
     5041 tctatttttg gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta
     5101 acaaatgatg cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat
     5161 gcggatattt tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta
     5221 gttggcttgt tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt
     5281 ggtaaaataa aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa
     5341 agttattcaa aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa
     5401 tatttattcg aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac
     5461 aaaaaaggtg attggcacca gttcagagca agagacaacg gatatacctg ttttatattt
     5521 acttcatgga atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta
     5581 tgtagcaaac tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga
     5641 tacagtttat gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca
     5701 tgaattattc ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat
     5761 gggcggatat ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat
     5821 ctctctttca ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa
     5881 agcatctttt tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga
     5941 tccgcaatac ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat
     6001 ggcttgtggg gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca
     6061 ggaacaaggc ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg
     6121 ggaccaatgg attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct
     6181 taaaaagata gctcctatag agatcacaaa catagtgatc tctataggag cttttttact
     6241 gctcgttttc tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg
     6301 ttagaaagag tgctttttca ttcaattctg catgggactg catactttct ttaaatccct
     6361 ctaattgatt ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat
     6421 ttccgtccat aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa
     6481 tcaataaatt gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac
     6541 gaacaagtaa ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag
     6601 tattgatttc taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca
     6661 tatactgcag caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt
     6721 ccattgtttc agccattttt tgcaagagct gttgtttgtt tttgaaatac caatataaag
     6781 caggtgcttg aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt
     6841 gtgtttcagc caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt
     6901 ccattttatc aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt
     6961 ctaattttac agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat
     7021 gaaattaatc ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc
     7081 cgtagcagtc atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga
     7141 agctattatt acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct
     7201 ggctttgcta ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca
     7261 aggcgtaagt gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg
     7321 aaatatcgaa cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca
     7381 aatccaaaat ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat
     7441 tggtgccttc attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct
     7501 tatcgttacg gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat
     7561 gatcattcaa aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat
     7621 tcgtgtagtg aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag
     7681 tgaggactta acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc
     7741 cttcatgctg gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt
     7801 taaagatgat cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat
     7861 tatgatggca atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc
     7921 gctgaagcgg attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc
     7981 agaacaagag ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga
     8041 ggatacagat acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat
     8101 cgtcggggcg actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga
     8161 tccgacagaa ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag
     8221 tttaagacaa acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc
     8281 tcaaaacttg cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc
     8341 tattgctcaa gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga
     8401 agaacgtagc agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt
     8461 tatcggtaat ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc
     8521 tgaaaagctg gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat
     8581 cgcacaaaaa attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag
     8641 attagtcggc gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat
     8701 ctttgaaact caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa
     8761 tttttctatc actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg
     8821 atcattgcta cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa
     8881 ttagctaatt acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct
     8941 gtaatatgga aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt
     9001 attttgttca cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac
     9061 aaattggaaa aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt
     9121 cgttttacca gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc
     9181 atcacgaatg ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag
     9241 ttagcttggg ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc
     9301 cgcaaagcac ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat
     9361 atggatgaaa aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg
     9421 attgaaggat tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct
     9481 tattctggtc tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt
     9541 atctttttcg gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc
     9601 ttagttgtga cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca
     9661 tctggataca gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt
     9721 gatgaaaaag atgaagtcaa accagaaaac ggtaaagcat ttgatggtgt tgaaaaaggt
     9781 cttgcgttgc agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc
     9841 tcgatcaatg tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa
     9901 acgacgatca tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc
     9961 gatggaatag atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt
    10021 ttgcaagaat ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg
    10081 gacgcttcag atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc
    10141 atgacgttag aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca
    10201 ggtcaaaagc agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt
    10261 ttggatgaag caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg
    10321 gatgaagcaa tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg
    10381 aatgcagata gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcat
    10441 gagttattag cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa
    10501 agttaaatta aaagaacctc gttataacgg ggttcttttt atatatttta tgttgtttgt
    10561 ttttataagg ggcagaaaag gggcaattta aatttctatt tttcctagtt tttctactag
    10621 ttgattttgc atatttttag ttgtatgagt ataaatcttt aatgttgttt ttgaatcttc
    10681 atgtcctaca cgatccataa tggcttttaa tgggattcct aattctgtaa gtagagaaat
    10741 gtgggaatgc cttaacatgt gagaagaaac ttttttatct aggtttaatt ctttggatac
    10801 tgctcttaaa gattgattat atgcttgaat agatatcggg ttaccatttt tagagatgaa
    10861 aatgtattta tctttattat tcttaattaa tttgttttct tcaattaatt gatctatgat
    10921 ttctttagca ttacttgata attcaatggt tctataagaa cctgtatttt tagttgttgt
    10981 tttaattttt aactcgtaat cataagtacc agaaaccgta atttt
//
LOCUS       DACDPS010000076        10792 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.076, whole genome shotgun sequence.
ACCESSION   DACDPS010000076 DACDPS010000000
VERSION     DACDPS010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.076"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            57..635
                     /locus_tag="IWJ07_002179"
     CDS             57..635
                     /locus_tag="IWJ07_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP9333722.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            635..1564
                     /locus_tag="IWJ07_002180"
     CDS             635..1564
                     /locus_tag="IWJ07_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9333723.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            1561..3297
                     /locus_tag="IWJ07_002181"
     CDS             1561..3297
                     /locus_tag="IWJ07_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9333724.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(3360..4196)
                     /gene="pheA"
                     /locus_tag="IWJ07_002182"
     CDS             complement(3360..4196)
                     /gene="pheA"
                     /locus_tag="IWJ07_002182"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAP9333725.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(4193..4702)
                     /locus_tag="IWJ07_002183"
     CDS             complement(4193..4702)
                     /locus_tag="IWJ07_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAP9333726.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(4695..5990)
                     /gene="aroA"
                     /locus_tag="IWJ07_002184"
     CDS             complement(4695..5990)
                     /gene="aroA"
                     /locus_tag="IWJ07_002184"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAP9333727.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(6003..7094)
                     /locus_tag="IWJ07_002185"
     CDS             complement(6003..7094)
                     /locus_tag="IWJ07_002185"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAP9333728.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(7111..8277)
                     /gene="aroC"
                     /locus_tag="IWJ07_002186"
     CDS             complement(7111..8277)
                     /gene="aroC"
                     /locus_tag="IWJ07_002186"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAP9333729.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(8279..9343)
                     /gene="aroB"
                     /locus_tag="IWJ07_002187"
     CDS             complement(8279..9343)
                     /gene="aroB"
                     /locus_tag="IWJ07_002187"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAP9333730.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(9376..10398)
                     /gene="aroF"
                     /locus_tag="IWJ07_002188"
     CDS             complement(9376..10398)
                     /gene="aroF"
                     /locus_tag="IWJ07_002188"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAP9333731.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         2988 a         2385 c         1990 g         3429 t
ORIGIN      
        1 tctcctagaa ataagtattt ttaaattaca agactattct taaggcgggt aaaaaaatgc
       61 caatacgcgt aattcttatc gatgatgaac agacgatttt agatggaatg aaacagttga
      121 tcaattggtc tgagcacggg tttcaattaa tagggacttt ttccagtccg ctgaaagcgc
      181 ttgatttttc tttggtacat gcaatcgata tcgtggtcac agatgtaatg atgcctgaat
      241 taaatggaat cgaattatcg cggctattaa aacagcaaca acctgaaaca cagatcctta
      301 tcctctctag ctatgatgaa tttgatatgg ttaaagattc ttttaaagaa ggcgtcgctg
      361 attatctttt aaagcctcga ttgagtcctg atttttttct aaattcctta actgccgttt
      421 cacaaaaaat caaaaaaaag cccaacaaaa gcccaatcac caatcgttat gagcagatct
      481 gcgagaatct ttcacagcag attgctggac aaactcgtcc attgcttgac cctcttcttt
      541 ttgaacacac cttgtttttc tgttatatac cgaaaaacgg ccattcagtt caaaacaagg
      601 ctctaaacaa aaacagtcga tcacccattc attgatggat agcgacgatt cgtttactat
      661 ttatcctgtt tctacctttt cggaagaatc cggctatgta atcaatactg attccaaaga
      721 tacacttagg aagtttgttc aaaagtttcg tcaagaagtt tttccaggtg atttttttgt
      781 tgtttctgat tcttttgcaa tccaagatat caatgaaata ttccaagaga tcagaaattg
      841 caccaaagga caacgtttct atcataaggg aaagaacctt gcttatcagc atgaacttct
      901 tccgttatta accaaccacg agcagcaagc aagtagttat ttaacaaaaa tcgttcataa
      961 agactacgct ttagctgtca aagaacttga gaatttcttc acgacgatat tggaacagcc
     1021 tattgatcca gcttatttga aacatgaggc aatcaatcgt ttctatgcat tactcaacgc
     1081 tttggctgac gagcatgttg ggaatgaaga actttctttg ctcaagatcg cccttccttc
     1141 tttattagcc gatgctgagt atttcgaaga tttttctatt cttgtcaaag aaacacttga
     1201 gaaactcacc cacttttctg caaatgcaaa aaaagacaat ttcgatatct tgactttgat
     1261 ctacgagttc gtccaggaaa attatgagaa agaaatcaat ttacaacttt tatccgaaaa
     1321 atatcatttt tcatatagct atctctcagc catctttact gaaaaatttg gtatcaactt
     1381 ttcaaaatat ctaaaaaaag tccgtatcag taaagccaag ctctttttga cggaaacacg
     1441 gcttactttg acggaaatct gtgagaaagt cggctatacc gaactgggtt atttctccag
     1501 agtttttaaa gaagagacag gaatgacacc ttctcaatac cgtaaaggaa cttttgtaaa
     1561 atgagaaaaa aaatcaaaga gcatgaactc tttgccaaac ttctgttcat tttgttcatc
     1621 ggtctttttc tgcaagccat cgtcatttct ttgtttattt atcatcgatc aagggatgcc
     1681 tatatccagc tgttcaatca atcgaatgat gtggtactaa aaaagattca atctgaattt
     1741 gaaagtttga atgatacaat cgagaataca ttagccgctt ttgattctaa tccagctgtc
     1801 aaaagctatt tttccaatga tccagcccaa catatggaac agcttcagca actaagaaca
     1861 atccaaaaaa tgaatgactc tctttcaaaa atccatccaa tgatcgatta cgatgtattg
     1921 atctttggag aaaatggaag aacttttgtc ggaaacgaca tgctcactgc cgtatctgcc
     1981 gattcttttt ttcaatctgc gattgctcag agagtgaatg aacgtgctgc tgatacacaa
     2041 atgctttttg cacaccacgg attgactctt cgcgataaga aagcacccag cgtcttcttt
     2101 gtcaaaaaac tgaaaaatac attgaatcac gtttacggat atgctgtttt atcgatctca
     2161 tccaagcaac tagcgaatct ttttcaatct gtagtaaatc ctgagatatc gaagatctcc
     2221 ttcatgaacg aagaacagac gatcatcgca tcaaatgaag aaaatacaat tgggagccga
     2281 tcaatgattt tcaaaaaact aacagtaggt gaaacaacga tttcaaatgg acagcagttg
     2341 acccgtcttc ctctgtaccg ccaggattgt acattactca gtcaagtcga tcttcattct
     2401 ttatctaacc aaatgggcgt gatttttccg attattttgt ataatatcgt gacacttgct
     2461 tttgctggct cattggtatt tatgtatttg gatcgacaca caaaatcgat ctaccgcttg
     2521 attgattcgc taaaacggct agaggaagat ggcttaaaag ctactgttcc tgtacaagga
     2581 agccatgaag tccaattgct agggaatacg atcaatcaat tgctaagtaa tgctcagcat
     2641 cattatgaac agacgatcca agacgaaaag aagaaacgtt cgcttgaaat ccaaactatg
     2701 caagcacaga tacagcccca ttttatttat aatacgctga ctagcttgaa atttttgatt
     2761 tggcagaaaa aaacagatga agcagttcaa ggaatcgaca gtttcattac tttactaaga
     2821 agtacgattg ggaaaaaaga agaagtgatt ccagtcaagg aagaaatcaa aagcgtacaa
     2881 agttacatcg atcttttgag tatgcgatat ggcgaaagaa tctcggctaa aataatgatt
     2941 cctgatgaac tgcagtcatt atctatgccg aatatgatcc ttcaaccaat tatcgaaaac
     3001 gcctatcttc atgcttttca caataaaacg caaggattca tcacggtgta cggcaaaaca
     3061 aaaaatcagc ttcttttatt cgaagtgatt gataacggcg atggcttcga tactgaaaat
     3121 accaaacaaa aaaatgatca tttttcagga atcggcatca gccatgtaga tgagcggata
     3181 cgcctaatgt atggcgatgc atatggctta acgatccaat cagtgattgg gacaggaacg
     3241 actgtaacga tttgtctacc gcttcttcct aaaaatcaat cagaatatca aaaataaaga
     3301 atagccaagc gctgattttt ttattttaag ttaaataaat caatcagtat tcttgacttt
     3361 catctacgcg tctcttctac tgtcagcact ggataacagc ccaatatttg actttctcct
     3421 ccgagcatct tgatttcttg gagcgcattc tctactaatg tcattggacg gtccaacaac
     3481 aaatcgatca cgaaaaaata ttctcccaag ctagttttca atggtcggga ttcaatctta
     3541 cttaagttga tttcccgcca gccaaaagct gccagcattt tatgaagcat ccctggccga
     3601 ttagcaggta gtgtcaagat gacactcatt tttactggag ctgactgctg gctggtcatc
     3661 tttcggtcac cgacgatcca aaaacgtgtc tggttcaact cattatcttg gatattctca
     3721 gctaagatct ccaatccaac atgctgtgct gtttctaatg aagcaatcgc tgctgcgtct
     3781 tctttcggat gttctgctac atacattgct gcagcagttg ttgattctgt tgctactaaa
     3841 ggaacattag gataatgcgt ttctaaaaac tgttgtgatt gtgctagtgc ttgtggatgt
     3901 gaaaggattt ttgtaatctt tgttgctgga ttgcctaaaa gctgctgctt gataggaaga
     3961 acgatttcac ttttaacttc gacttctgga tgtttggaaa gaagatcgat tgtgtgatga
     4021 acagctcctt ccaatgaatt ctctacagga acgacagcca gatcgacttg tttgcgaaac
     4081 aaagcgcgca aacaaacagg gatggaagca tacgcagtca attgttctgt aggaaatagc
     4141 tgacaagccg cctgataagt gaatgaactt tctggcccta gatagctgac gttcatatgc
     4201 ttttgatcct ttctataatg gtatcgatga tcttttctgg tgtttgattg gtcgtgttga
     4261 ttcgatgtgt cgcactttct tcataccaag aaagtcgttg tgccaataat gcttttattt
     4321 cttctcttgt ttttccaaga gctaaaggtc gaatagacgt ttcatcttgt tggatacgat
     4381 caactagaca gtccgcttct gcctctaagt aaagaacaat cggctgtgtt tttaaaaaac
     4441 gccgattttc tgcactttgg acaatccctc caccagtcgc gataattttg tcttcatttg
     4501 acagctttct taaaatatct gactccactt ttcgaaagct tttttcaccg taatagtgaa
     4561 aatattcggc aatcgacaat tggatatgtt cctcaatctt tgtatctaaa tctacatact
     4621 ctttttgtaa acattgagct aaccctttac cgattgttgt ttttccagcg cccatgaaac
     4681 caatcagcaa aatgctagcc atgagcttct cctcctgaca caagttctgc taaatcgtca
     4741 aaaaatgccg gataagagat tgaaacagct tcagctcctt ctagttcgca aggtccatca
     4801 gctatcaaag cagctacttg cagcatcata ccgattcgat gatcgcctct gctggacact
     4861 tttgcgccat gcagaggtgt cggcccatgg atgatcagac cgtctgctgt cgcttttatc
     4921 tttgctccca ttttctgtaa ttcttctgcc accgcatcaa ttctgttggt ttcttttact
     4981 tttaattctt ctgcgtcttt gatgatcgtg attccttctg cttgagtagc cactaaagct
     5041 agaatgggca attcatcaat cagacgcgga atgatttctc caccaatcgt tgctttcttc
     5101 aaatgactcg tcttgacact taaatcagcc gattggttct gttcattgtg attcgtctgg
     5161 gtaatcgctg cgcccatctc ctccaacaca tctaagatac cagttcttgt tggattgacc
     5221 ccgacatttt ttaacaacag ttggctttcc ggaacaagaa gtcctgctgc tagaaagaaa
     5281 gcagctgatg aaatatcacc tggaacagtt atctgttgac cagttaattt ttgcggtccg
     5341 gtcactatga tcgttttatc ttccactgtg atccttccgc caaattgacg aatcatttct
     5401 tccgtatggt tacgggaacg ttctttttca acgattacgg atgtgccttc tgcttgtaat
     5461 gccgcaaaca gcaaagcaga tttgacttgc gcactggcta caggcatatt gtatcggata
     5521 ggtgataaag agcgggttcc tgtcacagtg attggtagat actggtcatt ttcttctcct
     5581 tgcaagtcag cgcccatctc gcgtaaaggt tccatcactc gccccattgg acgtttagac
     5641 aacgaagcat ccccgaacag agtagtcgta aaaggctgtc cagccaaaat acccatcaat
     5701 aaacgtatgg tcgtaccgga gtttcccatg tcaattgatt cggttggttg gacaaatgag
     5761 tggctaccgc gaccatgaat cttgatcacc tcttcctctt cttcgatctc gacacctaat
     5821 tgttggaagg catgtaaagt actcagacaa tcttctgcgc gcaaaaaatt ttgtatagtc
     5881 gtcgttcctt cagcaattgc tccaaacatg atgctgcgat gggaaattga tttatctgct
     5941 ggtatcctaa cagtccctct taatccatgt atttgttgta ataattgcat ttatccagcc
     6001 tcctatttga tccagcaatg ataactggta ttagacataa tgattctttt tgcttgtaac
     6061 aaatcctgtt ggtttttaaa actgatttgc agtattccta aaatatcttc cctggtctct
     6121 tggatcttca aattaatcaa agaaactccc gcttttccga tcagtccagt tacttctgca
     6181 ataacgccag gttcatctgg cacatcgata aataaatcaa aaaaggcagg aatcgcgcct
     6241 tgcttatgga caggcaactg gtctcgactt tcttttgccc gattgaaaaa agaaaagatt
     6301 tccgattggt tctcagtcat gatccattct gctatttgtg tcatttcttg ttgccaatct
     6361 gccaatcgtt ccaataatat ctgcttattg ctcaacaaaa tatctgtcca catgacagga
     6421 tctgaggaag cgatcctagt gatatctcga aagcctcctg ctgccagttg tttggctcgc
     6481 ggatactctt gattgaattg ttctgcctga ttgactaagc cagctgccag aatatgtgga
     6541 aaatgactta gcattcctgt tatacggtca tgttctcttg ctgtaagagt tacatatttt
     6601 gctttggttc cgcaaaatag ttcttgcagt ttttcaacat cttttttcgt cttttccaaa
     6661 ggtgtaaaga tgtaataggc attttcaaat agtttagcat ctgctgcctg tatacctgat
     6721 ttatgagaac cagccattgg atgtccaccg acaaaccgaa gatttttctg ctcagcggat
     6781 gtcacgactt gctgcttcgt actgctgaca tccgtaatga tgacgtgatc agacaagttg
     6841 attctttcaa ggacatcaag ataagctaag gttgtacgga cgggaacggc taataagata
     6901 acgtctgctt gttctgcacc tgtttcaaaa tcacttgcaa cgtaatcaac gatctgctgt
     6961 tctactgctt gctcacgggt ttttgactga ctatcccaac caactactat cagttctgga
     7021 ttggtctgac gaatacaaag tgccatagaa gcaccaatca gtccaagccc cacgataaaa
     7081 acatgttgtg ccatcttatt tgcccccttt ttagtaatgt ttcaagtagt cacggtactc
     7141 cttcactgca gctttcaact caacaagcga atcacttggg aattttgaca atagctctgt
     7201 cgctaccaca gtggctacaa cattttccgc aacgacgcta gctgcaggaa ctgctgtact
     7261 atccgaacgt tctacacttg ctttgtaagg ttcttttgta tcgatatcaa cactttgaag
     7321 tggcttgtac aatgtaggaa tgggtttcat gaccccgcga atgacaagtg gttcgccatt
     7381 ggtcattccg ccttcgaagc cgcctagatt attggtacgt cggaaatagc ctctttcttc
     7441 ttcccatagg atctcatcca tcacttctga gcctggtaat gaaccagaag tgaatccgac
     7501 cccaaattct acgcctttaa aggcattgat actaaccaca gcttgtgcga ttttggcatc
     7561 tagcttcgtg tcccattgta cgtagcttcc taatccagct ggaacgcctc caacgagcac
     7621 ttctacaatt cctccgatcg tatctccatt tttctttgtc tgatcgatca aatcttttat
     7681 tctttgctca actgctggat caacaactcg tacttctgac gcttcagaaa tttctttgat
     7741 ttcttgaatc gagatatttc caggtatttc tgcttggatt ccaccaagac aaatcacgtg
     7801 tcctgctacg atgatgtcaa gttcttccag aattttcttg gcaattgcgc cgattgctac
     7861 acgcatggtc gtctctcttg cagaggaccg ttcaagcaca ttccgaagat ctcgatgatg
     7921 gtatttcata ccgcctacca aatccgcgtg tcctggacgc ggacgtgcaa ctcgccgcag
     7981 tgattggtct ttttcaggca cttctgctac agacatgaca gttgtccaat ttttccaatc
     8041 tttattttct accactaaag tgacaggtga accaattgtt ttgccgtgac ggatgccaga
     8101 cgtgatccga acacgatctt tttcgatctt catccttcct cctctgccgt aaccagcttg
     8161 tctgcgagcg agttcgtgat tgatttcttc ttctgacaga ggtaatcctg ctggtagtcc
     8221 ttcaataatg gctgttaatt ctggtccatg ggattctccc gcagtcatat aacgcatctt
     8281 acagtcctcc taagtaatct ttgaatgtct ctgtgggaac aggaacgatt tttgcttgcc
     8341 caatcttttc tagcaagatg atattcaatt ggcttcctct tgctttctta tcatgggtta
     8401 tcgcgtggaa taattcatct ggatgactta gatcgtacgt aatcggcaga tggaattttt
     8461 gaagtatcct ttctaaagca gctgtcgatc cagttggaga caagccgatt ttttctgcat
     8521 gagaagtgat ggctaccatt ccaatagcta ctccttctcc atgactgact accccatatc
     8581 ctgctgtatt ttcaattgca tggccaattg tatgaccaaa atttaaaata agtcgatttc
     8641 cttggtcaaa gacatcttct tccactactt ttcgtttgat ttccaacgta gcagtaatga
     8701 ttggaacagc atgttccaga agatcttttt cgtctttcag ctcgttcagc tgtttccaaa
     8761 gcgttgcatc tgctatagct gctgatttga tgatttcagc gattccttct cgtaaacgac
     8821 gtaccggcaa agtttttaga acctcaggat caatcaatac accagctggc tggctaaatg
     8881 ttccgacaag gtttttcgct ttgtttgtat tgactgctgt cttcccgcca atactagaat
     8941 ctacttgtgc aagtagcgta gtcggaattt gaacgaaatg gatcccacgc atgaacgtcg
     9001 aggccacgaa tgctgctaga tcacccacca ttcctccacc aagtgcaagt atcccatcgc
     9061 tgcgggtaaa atcattatct gctaaaaaat cgtataactc agctgcttgt tctaaagatt
     9121 tactggtctc acccgccggt acgattgaaa ggactggttc aaaattagct tgtctcaatt
     9181 ggaacatgat tttttctgca tatagtggag caacgtttgt atcagtgatc acagctactt
     9241 tttgaggtct ccaaatcgtt tgaagccatg atccagcctg ttctagtgcg cctttttcaa
     9301 tatgaatatc gtatttttgg ttatcaacat gtacagtaag cattcgtttc acttctttct
     9361 tttaaaaagc tggaattaat cgagtgtctg aatcttttcc attgcttctt tcatgatcgc
     9421 aacttctttc atcatgcgtg agtaagtctt ttcattcaac gattggggac cgtctgacca
     9481 agcatttgca ggatcaggat ggatctctac gatcatgcca tctgctccgc ttgccacacc
     9541 cgcacgagcc attggtggta caagttccca aattcctgta ccatgacttg gatcaacaat
     9601 cacagggaaa tgagttaatt ttttcatcaa tgggacagca cttaaatcaa acgtattacg
     9661 tgtcgctgtt tcgtaagtcc gaatcccacg ttcgataaaa atgacaggag atttgtcttc
     9721 gactgcaatg tattctgcag cattaagcca ttcattgatc gtaccggaaa ttccacgttt
     9781 caatccaaca ggtttacctg ttttaccgac agcagatagc agtttaaaat tttgcatatt
     9841 acgtgcgcca atctgtagga tatcgctgta ctcagccacc atatcgatat gcccttcatc
     9901 catcacttca gtaatcactt tcattccaaa ttcatcagcc gcttgacgga tgtatttcaa
     9961 tccttcttct tctaaacctt ggaaagcata tggagaagta cgtggtttga aggcaccacc
    10021 tcgcagaatt tttgcgcccc cggctttggc cattcgcgca cattctctga tttggtctaa
    10081 tccttcgata gaacaaggac cagccatcgt cacgaaactg ccgtcaccaa tttttactcc
    10141 gtctacatcc acgactgtat tgccaggatg gaattctcga gaagtcaatt tgtatgtgtt
    10201 taaaatacgc acagcatttt ctactccttc atagcgtgta aatgctcctt cttgtaaact
    10261 tcgcgtatca ccttttagtc caatcactac tcgttctgtt ccttgatcga ttgagacttt
    10321 gaatccttct ttttcgatat gatcgacgac tttttttatt tgttcttccg ttgcttcttt
    10381 tttcataatt actatcattg tttttcccgc tttcttttgt ttacttatct atcttggctg
    10441 attttctcta ctttttcaag gtggttttac attaattcct actaaatgac cgtgtcctgt
    10501 tgttctcatt ttcgctcctt ctttcctact tatgacccag ataatcaaca aaaaaagatg
    10561 tccctgtagg aaattgcctc tacagtgaac atcttcaacg agccttaaaa agcgctgcaa
    10621 aaatataaaa tagccactat agatgaattt ctctacgtgg ctgtaagtcg tgtttaaaaa
    10681 tctttagcca tcatagatac aaacctggtt tgtttgtacc cacgaagtca tgaatacaaa
    10741 cctatctaaa atagctaaag taatacgtat tcagttttgt aagttttcta ga
//
LOCUS       DACDPS010000077        10791 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.077, whole genome shotgun sequence.
ACCESSION   DACDPS010000077 DACDPS010000000
VERSION     DACDPS010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.077"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            135..950
                     /gene="thiD"
                     /locus_tag="IWJ07_002189"
     CDS             135..950
                     /gene="thiD"
                     /locus_tag="IWJ07_002189"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAP9333732.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(1279..2730)
                     /gene="cls"
                     /locus_tag="IWJ07_002190"
     CDS             complement(1279..2730)
                     /gene="cls"
                     /locus_tag="IWJ07_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP9333733.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            3071..5737
                     /locus_tag="IWJ07_002191"
     CDS             3071..5737
                     /locus_tag="IWJ07_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9333734.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            6101..6853
                     /locus_tag="IWJ07_002192"
     CDS             6101..6853
                     /locus_tag="IWJ07_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9333735.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(6875..8029)
                     /locus_tag="IWJ07_002193"
     CDS             complement(6875..8029)
                     /locus_tag="IWJ07_002193"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAP9333736.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            8214..10655
                     /locus_tag="IWJ07_002194"
     CDS             8214..10655
                     /locus_tag="IWJ07_002194"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAP9333737.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT         3519 a         1916 c         2312 g         3044 t
ORIGIN      
        1 atgttttttc aaataaattt gattaccaag aaaaggtagt ggtatattta ctgagggata
       61 ctttgaaaaa catatgatag aaacagtaac agtattatga aaaatctttt tataaaaatg
      121 gaggaatcga tttgatggaa gatcagtatg tacaagcaat gacgatcgca ggatcagata
      181 gcgatggaag cgcagggatg caagcagata tgcatacatt ctttacaaga aatgtttacg
      241 gtgtttctgt catgaccgca tgtgttgcag gtaattctta cggaatcgga gcaagtgtga
      301 cattgccaac agattttatc gacaaagaat ttgaactgat tgcgaaggac tttcaaatac
      361 gtgcagctaa aacaggtatg ctagcagatt caaagttgat tgagacagtt gtaaaaaatt
      421 accaaaaata tgattttgga ccattagtcg tggatcccgt aatcgtaacg aaacatggaa
      481 atcttttact agaagaatct gctttgcaaa gcttgaaaga aaagcttgtc cctttggccg
      541 aagtactgac acctaatttt tatgaagcag aaaagctgac tggacggaca ttagcagctg
      601 aagaagattt tgtgcaagca gcaaaagaac tgcaagatat gggtgcaaaa aacgttatga
      661 tcaaaggaca tcatgctaaa aatgcgcagc aagaaacagt caatgactac gtattgttcg
      721 aatcaggaaa atcattttgg ttaaatgcac cattttatcc taccgaccgg atcaacggaa
      781 ctggcgattc gctatctgca tgtattgcag ctgagttagc gaaaggaaaa acagttgaac
      841 aagcgatccg attggcaaaa gactatgtaa accgtgcaat tcgtgaagaa ataaatgtag
      901 ggcacaaata tggccccatc aatcattggg cagctggtga attagagtaa aagaaagaag
      961 ttgtgaaaaa gttgccctgc atcaagtaat aagaacagcc aagtaaaaac agcttcttat
     1021 gtttttaact tggttggact tattaccgca gatgcaagct tttgaacacc gtttaagaaa
     1081 gaggtcgtga gcctgccgtc cagctccgag tcacaaggaa caagttcaaa aaacagcttc
     1141 tcatgtttgt tgaatattgt gacttgtgac cgaggagttg gcaggagaac accgtttgtt
     1201 cggaagaagg gactgtgaca agatttttgt cacagtccct ttaatcttag tatatttttc
     1261 tctacttgaa taaaaaaatt ataggattgg agaaaataat cgagaaattt gttgcttgaa
     1321 taatagccag ttagacattt ccttgactgt ttcagttgtc atggatgaac aatgtgtctc
     1381 atcttctttg aactgagtag caagttcttc taaaaactca ggatcgtaaa tgacagcact
     1441 tgtttcaaaa ttcaatcggt agctacgaat atcttgattt gctgaaccta ccatgcagat
     1501 ttcatcatcc attatcaatg tttttgcatg gaggaagccg ttctcataga caaggatttg
     1561 tacattttcc ttgatcagct gccgagcata atattgtgtt gctcgataaa taaaaggatg
     1621 atccggtttg ttgggaatca tgattttcac atctacacca gaggcagttg cgatttttaa
     1681 agcagcaatg acactttcat caggaacaag gtaaggtgtc tgtatccaaa cacgtttttt
     1741 agcagaagta atcaatttga taaatgaaag cttgatttgt tccctttcgt tattcggtcc
     1801 gcttgaaaca agctggatgg atgtatcagc aagcttagat tcatctcgat ctgctttttt
     1861 aaagaaataa ttcaaatgat agccgacctt tttttcttca ggtaccgaga cgttccagtc
     1921 tgttaagaag cgaagctgga gcaaagaaga agcggcaccg aaaatccgta tatgcgtatc
     1981 gcgccaatag ccgaattttt ttgttgtatt tacatattga ttggcaatat tgaaaccacc
     2041 ggtataacta atctttccat cgatcacaac gatttttcgg tgatcatggt aattcaatcg
     2101 gaaacgaagt aatgcccttt gtgaggtaac aaatgtatgg acatgtccac catttttgat
     2161 tagacgattg aaatcttttg cttttgtgcc atgagagcca aatgcatcat ataatattcg
     2221 aacttccacg ccttctgcgg ctttttcttc taaaacatgt aagacttgct ggccgatatt
     2281 atctgtcaca aatgcgtaat attcgatatg gatcgaatgt ttggcttttt ttagatcttg
     2341 aagcagtgaa tccaatttct cttgtccgtc tgtgagaaga gtgacagaat tcattcttgt
     2401 cagcggcata cgatttaatg aagagaagaa gtcaacaaat tgctgtttgt ctttatcgcc
     2461 catgtcagga tcgtaatgtt caatggtatc tcctctaaaa gactgaaagt tttctaattc
     2521 tttcaagtca ctttgtcgga gatagaattt tttcttatcc gttaatccac gtccgaaaaa
     2581 taaatataat acaaagccca ccccaggaag ggcaaacaat actaagagcc atgcccaaat
     2641 ggctgctaca tctcggggtt tgatcaaaat agccaccaaa gcaataagtg catttaatag
     2701 ataaagggcg gttataatac tagataccac aaaaatgttc ctccttacat acaataacat
     2761 tagtatagca tgatagaaga aagaagccta ccaaggtgaa tatacttttc actcctttca
     2821 tgaaaatgta ggtaaatcgg gctaaagtag ttgctttttg taggttaata aggtaggata
     2881 ttagtacagt tgaatatctc cttaggggag taaccggcaa cgtttgttgc gataatatca
     2941 acagtaagtt aaccaaatgg ttttctggta ttgtatgaaa tggtgagacc taagacaaat
     3001 gcgtatttgt cttaggtctc ttttttttca gacaggacta gggccaagat caaaaaatgg
     3061 gggatgaaat ttggaaaaca aggaacaagc acaacaagcg gtctataaag aaacgatcga
     3121 ctcgttgatg cagcaaacgc agagctcaac cgatggattg agcaccacgc aagcgaagga
     3181 aaaattagaa agagatggct tgaaccagct aaaagaaaag ccgaagaagc caacttggaa
     3241 attgttctta gaaactttca aagatgcaat ggttatcgta ttgctgattg tcgcactgat
     3301 tcagatggtc atgggtgact acatcgaatt cttagttatt tttgcagtat tgatgttgaa
     3361 ttctataatc agtgttgtcc aaacaaaaaa agcagaaggt tctttagaag cgttgaaaaa
     3421 cctttcagcg cccgatgctc gagtgattcg tgatggtaag gaaaaaacga ttccggcaaa
     3481 cgaacttgtg gtaggagata tcgtgttgtt agacgcaggc gattatgtac ctgctgatgg
     3541 acgtttacta gatgcgggat cattaaaagt agatgaaggt atgctgacag gagagtcgac
     3601 ttctgcagaa aaagaagtaa cggatatcac aagtacggta ccaatcggag atcgtacaaa
     3661 tatggtattc agtggaacga tcgtaactta cggacgcggt aaattcttag tgacttcaac
     3721 tgggaataat acggagatcg gacaagttgc tggccttttg gaatcaacaa ctgagcaagt
     3781 cactccgtta caacgaggtc tggataaatt cagtaaaaaa ctaagtttgg ctattttggc
     3841 attgtcttta ttgattttag gtatccaact gtttcgtatt tatcttggag aaggtacagg
     3901 agatatgact gcagatatcg tcagcgcagt gatgtttgcc gtagccgtag ccgtagctgc
     3961 tattccagaa gcattacagt ctatcgtaac aatcgtgtta tctttgggaa cgaataaaat
     4021 ggcaaaacgt catgccatta ttcggaagct tccagcagtt gaaacacttg gttcgacaag
     4081 tatcatctgt acagataaga ctggtacatt gacacaaaac aaaatgacag ttgttgacta
     4141 ctttttaggt aatggcgata ctggggattt cacaaatgac ccttcaaaat ggacagctga
     4201 cgaacgtcgc ttgatcgaaa tcagtgtttt agcgaatgat gcatcgatca gtgaagacgg
     4261 tactaaattg ggcgacccta cggaagtagc gtttatcgac tttagtgaaa aattaaacca
     4321 gccttatcag gaaatccgaa acaactaccc aagacaagca gaacttcctt ttgactcaga
     4381 tcgaaaatta atgtctactg gacacacaat tgataacaaa aattacttgt ttgttaaagg
     4441 cggaccagat atcgtcttcc aacgcagtaa aaaagtattg ctaaatggtg aagtcgttcc
     4501 gatgacggat gaattgatga aaaaattcca agagcaaaat gaagcttttt caacacgcgc
     4561 attacgtgta ctagcttttg cttaccgtcc acttgataat tttgatttat catttgaaga
     4621 tgaagacgat ttgatccttg ttggtttgct tgctatgatc gatcctccac gtgctgaagt
     4681 gactcaggcg gtaacagaag caagaagtgc aggaatcaaa acaatcatga ttactggtga
     4741 ccacaaaaca acagctgttg ctatcgcaaa acaaatcggg atttttgaag aaggaaacct
     4801 ggctttgacc gggacggaac tagatgaatt gacagaagat caactaaacg aagttttaga
     4861 aaaagtcact gtttatgcac gtgtttcacc tgagaataaa attcgtatcg ttcgtgcttg
     4921 gcaaaataaa ggtcatatct cagctatgac aggtgatggt gtaaatgacg caccagcatt
     4981 gaaacaagca gacatcggaa ttgctatggg aacgggtaca gatgtggcaa aagatgcagc
     5041 agcgatggta ttgacggatg ataactttgc ttctattgtc agcgcagtag aagtaggacg
     5101 taatgtgttt gataatatca agaaagctat ttcttatcta tttgcaggaa atttaggtgc
     5161 gatcattgcg atcatctttg ccttagttgc tgactggtcc aatccattta cagcattaca
     5221 actgttgttc atcaatttag tcaatgattc actgccagca attgcattag gtacagaaaa
     5281 agctgaacct gctacgatga accgcccgcc aagagatcca aattcaggaa tctttacgga
     5341 caaaacattg atttctgtaa cgtatcgtgg tgtgctgatt gggattgctg ttatctttgc
     5401 tcaatggctt ggcatgcagc attcacctga attcggtgta gccatgtcat tttcaacatt
     5461 gatttggtgt cggacattgc aaacattccc agcacgttca aacacacaaa cagctatcca
     5521 agcagggttc ttctctaaca gaagtgtctt actagctgta gttgtatgta gcgcgcttta
     5581 ctgcttaaca atgatcccag gactgaaaga agtcttctct atcccatcag catttggatt
     5641 gacagaagtc tttacatgta tgggaatcgc acttgtgaca attgttttga tggaaatcac
     5701 taaattgatc attgtacgag tacaaggaaa taaataaaac gaatcaacga ggttgtgagc
     5761 ctgccgttta attctgagtc acaaggtgca atttcaacaa acagcttctc atgtttgttg
     5821 aatattgtga cttgtgaccg aagagttggc aggagaacac cgtttaagga agaagttgta
     5881 aaatcagcat tttgagctaa tactgagggt catctgattg gacactgttt attcggaaag
     5941 agggactttg acacgacttt ggtcacagtc ccttttttta tttatgatag acggaaagcc
     6001 aatcatttta ttattcatca aaagagatag aatagataaa agaggagaat gatttattcc
     6061 gaaaagtgaa aacatataac aaaaaattag aggaaatcaa atgaatgaaa aagaatggga
     6121 tgaatttgct gaagaatatt atgaaatcca gcaagaatcg caagtaacta ttgctgaaga
     6181 tgtaaaaaat tatctgaaaa cggagaaagt cctgcctgct gaaacagcag cagacgttgc
     6241 tggaggatct ggcagatttc tttccttagc aaaagaaatt aatcgctatg atttgatcga
     6301 tttttcttca ggaatgctag aaaatgcaaa aaaagaagca gaaaaaaatg agttgccaaa
     6361 tgtttccttt atcaaacagt cgttagaaga atttatccaa tcaaatgaga catatgaaat
     6421 ggtttttaca gcagcaaatc cagcattggt acatcttatt caattagaaa aattgatgaa
     6481 aaagtcaagc aaatggtgcg tgataatcag agtagtggaa tcaatcgatg atgtttttta
     6541 tcctatcgaa aagcaactag gaatgataga agaagatcct aatacatcgc cagttgtaat
     6601 ggatcaattt gaaacatact taattgatga aggattcaaa gtgaagaaaa aagattttac
     6661 ttattttaca caggaatcaa ttactgaaga attgatgaag ctatactatg acgcgtacgc
     6721 aaaagatcct gttgtgacca ggtacattcg caaactattc agtgaaaaac gagaaatcct
     6781 tagtacgact aagctgacct atagactgct cctttggtcc gcagataaaa aaagaacctt
     6841 tcacgactcg tgaagggttc tttttatgat tttattatat tttgtagtaa cgaatatcat
     6901 tttcgatctt tgtaatcgaa aaaggtacat ctgaagtata ttctgcacat tctccgtgtt
     6961 caggaagcgt ctcactgaaa atcgtttcat attcgtcaac agtgatccgt gttcgagaat
     7021 ctagcatctc ttgatgagat tgtgcgaaga gatattcttg atagttttct tccagataac
     7081 ctgtaaaaaa ttcacttact gcaccagaac cgtagctgaa tagaccgatg cgatctcctg
     7141 gctgtagtga tttcgaattt tccagtagag aagttagccc caggtataat gaaccagtgt
     7201 aaagattacc gattcgtcgg ctgtaacgga tgctttcttc atagcgagcc ataagacgtt
     7261 cctgattttc ttcgtctgtt tggtctaata cgctttgcaa tgcctttttt cccatcttag
     7321 tatacggaat atggaaagca atcgcctgat aatcttcgag tcctcgaccc gacaattctt
     7381 tatgtcgatt ccaaactttt tggaatgatt cgatatacgt agaattagat aaaggaccat
     7441 caacaacagg aaattcgcta taatctggac gccagaaatc atagatatct tctgtcagaa
     7501 atacgctgtc gtcttcaatc gataaaatac gcgggttttg agtgatcatc atcgcaacag
     7561 caccgacacc ttgcgtcact tcaccaccgc ttgccaagcc gtaacgagca atatcacttg
     7621 ctatgactag tacttttcgt tctggatgat ttttgacata ttctttcgcc atgtgcaaag
     7681 ctgcggtgcc accgtagcaa gcctctttta cttcaaaaga acgagcaaaa gattggattt
     7741 tcagtaaatg gtggataatt accgcactcg cttttgaatg atcaatcccg gattctgttc
     7801 cgacgatgac catgtcgatt agctcacggt ctttttctgt tacgattttg ctggcagcat
     7861 ttgctcctag tgtaatgatg tcttcatttg cacgattgac tgccatttgg tcttgaccaa
     7921 tccctatatg gtattttgca ggatcatccc cacggctttc tgccaactct gtcatatcta
     7981 aatataaatt aggaataaaa aaggaaagac gatcaatccc tattttcatt tcaacacctc
     8041 tttacttatg ataaaaaact tactttacta aggtatcata aaaagattaa aagaaatata
     8101 aaaaagagaa tatttaagga ttgttaaaat ttaatcttag aaaaaaataa ctggaacaag
     8161 agctatttgt ttttacagca aagaatggta tatttttgta ggaggtggct tacttgaaag
     8221 aagtcgttat gatagatgct gcaagaacgc cgataggaaa gtatcgcgga agtttaagtc
     8281 catttactgc agttgaatta ggcacacttg tgactaaagg attattagat aaaacaaaac
     8341 tgaaaaaaga taaaatcgat caagtaatat ttggaaatgt cctgcaagct ggtaatggtc
     8401 aaaatgttgc cagacagatt gctttgaaca gcggtctccc tgtagatgtc ccagcaatga
     8461 cgatcaatga agtttgcgga tctggtatga aagcagtgat cttagcacgc cagctgatcc
     8521 aattaggtga agcagaactt gtgatagccg gcggaacaga gagtatgtct caagcaccta
     8581 tgctgaaaca gtatcagtca gaaacaaatg aatatggtga accaatttcc agtatggtca
     8641 acgacggatt gactgacgca ttttcaaatg cacatatggg attaaccgca gagaatgttg
     8701 caacacaatt ttctgtgagc agagaagagc aggatcgcta tgccttgtcg tcccagttga
     8761 aagcagcaca tgctgtcgaa gccggtgtat tttctgagga gatcatccca gtcaagattt
     8821 ctgatgaaga cgtgttatct gaggatgaag cagttcgtgg aaatagtaca ttggaaaaac
     8881 tgggcacgtt acgtacagta ttctcagaag aaggaactgt aacagcagga aatgcttccc
     8941 cgttgaatga cggtgcctct gtggtgatcc ttgcatccaa agaatacgca gaaaataata
     9001 atctgcctta tttagcaacc atcaaagaag tagcggaggt cggtattgac ccaagtatca
     9061 tgggaatcgc tccaatcaaa gccattcaaa aattgacaga tagatcagga atgaatttat
     9121 caacaatcga tctttttgag atcaatgaag catttgcagc ttcttctatc gtagttagcc
     9181 aagaactgca gttagatgaa gaaaaggtca atatatatgg aggagcaatc gcattaggcc
     9241 atccaattgg tgcaagcggt gcacgtatct tgactacatt agcttacgga ttgcttcgtg
     9301 aacagaagag atacggtatt gcttcattat gtattggtgg aggtttaggc ttggcagtct
     9361 tgttagaagc gaatatggag caaacacata aggatgtgca aaaaaaaaag ttttatcagc
     9421 ttacgccatc tgaacggcgc agtcaattaa tagaaaaaaa tgtattgaca caagaaactg
     9481 cgttgatttt tcaggaacaa acactttctg aagagctctc cgatcacatg atcgaaaacc
     9541 aagtgagtga agtggaaatc ccaatgggga tcgcgcagaa ttttcaaatc aatggcaaga
     9601 aaaaatggat tccgatggca acagaggaac catctgtcat tgcagctgca agtaatggtg
     9661 caaaaatctg tggaaatatc tgcgcagaaa cacctcagcg gctgatgcga gggcaaatcg
     9721 ttctatcagg aaaatcggag tatcaagcag tgatcaatgc agttaatcat cgaaaagaag
     9781 agctgattct ttgtgcaaat gaaagttatc cttcaatcgt gaaacgcggg ggcggtgttc
     9841 aagatatctc tactcgggaa ttcatgggga gtttccatgc atacttatct attgattttt
     9901 tagtggatgt aaaagatgca atgggcgcta acatgatcaa ctcgattctt gagagtgtgg
     9961 caaacaagtt acgggagtgg ttccctgaag aagagattct attcagtatt ttaagtaatt
    10021 ttgctacaga atcattggct tctgcctgtt gtgaaatccc attcgaacgc ttagggagaa
    10081 acaaagaaat cggtgaacaa attgccaaaa aaatccaaca ggcaggggaa tatgcgaagc
    10141 ttgaccctta ccgtgcagca acgcacaaca aagggatcat gaatggtata gaagctgtag
    10201 tggcagctac aggaaatgat acacgtgcgg ttagcgcttc gatccacgcc tacgcggcaa
    10261 gaaatggatt ataccaagga ttaacagact ggcagatcaa aggggataaa ctagttggaa
    10321 aactaacggt gcctttggct gttgccacag taggcggagc ttccaatatc ctgccaaaag
    10381 caaaagcatc tttagctatg ttagatattg attcagccaa ggaactagca caagtaattg
    10441 ctgcagtagg gttagctcaa aacctggctg ctttgcgtgc attagttaca gaaggaatcc
    10501 aaaaaggcca catgggactt caagctcgtt cattagcgat ctcaattggg gctattggtg
    10561 aggaaatcga gcaagttgcg aaaaaattac gtgaagcaga aaagatgaac cagcagacag
    10621 ctatacaaat attggaaaaa atccgggaaa aataaaaaag caatatcttt agaagaataa
    10681 agcaagattt acagttattt tatttactgc agatcttgct tttttctcta tcagaaaata
    10741 tgtttattat tagaattttt tatagttaag gttaggcatc ttaagaaatc a
//
LOCUS       DACDPS010000078        10525 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.078, whole genome shotgun sequence.
ACCESSION   DACDPS010000078 DACDPS010000000
VERSION     DACDPS010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.078"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            23..646
                     /locus_tag="IWJ07_002195"
     CDS             23..646
                     /locus_tag="IWJ07_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333738.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1095..2144
                     /locus_tag="IWJ07_002196"
     CDS             1095..2144
                     /locus_tag="IWJ07_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9333739.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2141..3022
                     /locus_tag="IWJ07_002197"
     CDS             2141..3022
                     /locus_tag="IWJ07_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9333740.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3025..3795
                     /locus_tag="IWJ07_002198"
     CDS             3025..3795
                     /locus_tag="IWJ07_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9333741.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4074..4928
                     /gene="aroE"
                     /locus_tag="IWJ07_002199"
     CDS             4074..4928
                     /gene="aroE"
                     /locus_tag="IWJ07_002199"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAP9333742.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5106..6269
                     /locus_tag="IWJ07_002200"
     CDS             5106..6269
                     /locus_tag="IWJ07_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP9333743.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6386..6796
                     /locus_tag="IWJ07_002201"
     CDS             6386..6796
                     /locus_tag="IWJ07_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP9333744.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6970..9198
                     /gene="topB"
                     /locus_tag="IWJ07_002202"
     CDS             6970..9198
                     /gene="topB"
                     /locus_tag="IWJ07_002202"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9333745.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9336..9653
                     /locus_tag="IWJ07_002203"
     CDS             9336..9653
                     /locus_tag="IWJ07_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333746.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            9813..10049
                     /locus_tag="IWJ07_002204"
     CDS             9813..10049
                     /locus_tag="IWJ07_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP9333747.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10046..10444
                     /locus_tag="IWJ07_002205"
     CDS             10046..10444
                     /locus_tag="IWJ07_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP9333748.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3666 a         1768 c         2230 g         2861 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aattggcgca gctggcgtag ataagttgta ttaatggtga gactttccca
      901 tagatacaac tttttagagt gcatttctaa agcttgtatc tatgctagac atgaacattg
      961 actgccgcat agattagaat ctatgcggtt ttttgtgtaa agacaatgac cgctttggcc
     1021 attgtctttt tttgtagact ggatacttgc ctgattataa taaataaaaa gattgacttt
     1081 gcaggaggaa aaaaatgaaa aacaaacgat taataacagt tgtagtaatt attttagttt
     1141 atttagcagg tgcatttatt tatggaaaag cctcaggcga aaaagaaagt acttcgacga
     1201 accagacaaa aaaaacaaca gttgttgggg tattgcaata cgtgagccat ccggcattag
     1261 atgagattta tcgaggaatc aaagatggtc tgaaagaaga aggattaaaa gaaggagaaa
     1321 atcttgagat caaatttcaa aatggacaag ccgatcagag caagctagcc acaatgagtc
     1381 aacagctagt acaatctgat ccagatgcct tgattggaat cgcaacacct gccgctcaat
     1441 cattagcaaa cacgacttca tcgatcccag ttgtgttagg tgctgtaacg gatcctgtgg
     1501 gggctggatt agtcgcttct ttagagaaac ctggtggtaa tgtaacaggt gtttctgatc
     1561 agccgcctgt tacttcacaa atcaagcttg cttctgattt gttgccaaat gcaaaaaaag
     1621 tcggaatctt gtatgcttct tcagaggata attcaaaata ccaagtagca caagcagaag
     1681 aagctgcaaa aaaagaaggg ttagaatcag taaaatatgc ggtgccttcc actaatgaga
     1741 ttgcacaaac ggttcaagtg atgagccgac aagtcgattt tatctatgtt ccattagata
     1801 ataccattgc caatgcgatg caaacagtgg taaaagaagc aaacaaagca aatatccccg
     1861 tcattccatc tgtggataca atggtggaac aaggtggact agcaacaatc gggatcaatc
     1921 aatatcaatt agggcttcaa agcggtaaga tggcggcaga acttgcgagt ggaaaagaaa
     1981 aaccggaaac gacgcctgtt tatacatttg atcaagggga tacagtgatc aatcaatctc
     2041 aagctgatcg cttaggaatc attgttcccc aatcgatgaa ggaaaaagcc aaaatcatta
     2101 cagatgactc gcagcaagaa acatctaagg aggatgacaa atgatcgtat cagctatttc
     2161 acaaggcatg ctttgggcaa tattaggatt agggattttc atgacttatc ggatcttaga
     2221 ctttcctgat atgacgacag aagggtcctt cccattaggt gctgctgtct gtgtgacagc
     2281 tattactcat ggggtagcgc cgattgtagc tacattatta ggcgtaggtg ctggcatgat
     2341 agcaggactc gttacggggt tactttatac aaaaggaaag ataccggtta ttttagcagg
     2401 gattttagtg atgtcaggat tgaactcagt tattttgttt gtgatgcgaa caccaaatct
     2461 ttctttattg aatcatccga ttctacaaga cagcttccat cgctttgggt tacctgatta
     2521 ctttgacaca gtccttcttg gcttcatctc tttgggcatc ttgattgctg tactgctatt
     2581 cttcttcaat acagatttgg gtcaaggcta tattgcgaca ggagacaacg aaacgatggc
     2641 tcggtcgtta gggataaaaa ccgatcgtat gaaaatttta ggtttaactt tgtcgaacgg
     2701 tgtcattgca ttatccggtg cgctgattgc ccagaacgac ggatatgcag atgtaaataa
     2761 agggattggc gtaatcgtca tcggtcttgc ttccatcatc atcggtgaag tgatcttcca
     2821 agaattgacg ctagcagaaa gattgattgc tattgttgtc ggcagtatca tttatcagct
     2881 attgatttta gtagtaatca aactagggtt tgacacgact tatctgaagt tgttctccgc
     2941 tatcatctta gctatttgtc tgatgatccc gcaattgaag aaagcactga acttaaaaac
     3001 gggttttgag aaggaggcat agtaatgact gatatattga caatcaaaaa tggcagaaaa
     3061 gtcgtaaaca atggaatgaa tgaagagaaa gtactattgg atgaaattaa ccttacaatc
     3121 agagaagggg acttcatcac tgtccttggc gggaatggtg ctggaaaaag cacactgttc
     3181 aatacgatcg ctggtacgct tcaactcagt caaggatcga tctcttttaa agatcagctg
     3241 atcacaaaag attcagaaga aaaaagagca cgattccttt ctcgagtttt ccaagacccc
     3301 aagatgggaa cggctcctcg gatgacagta gcagaaaatt tgctacttgc acaaaaaaga
     3361 ggtcaaaaac ggaaattccg tttacgtaac ttgaaaaatc aaaaagaaga ttttttcaga
     3421 ctatgtcaag aagtcggtaa tggcctagag caacacttgg atactccagc gggtgaatta
     3481 tctggtggtc aacgccaagc attaagcttg ctgatggcga cgatcacagc tcctgagtta
     3541 ctattattag atgaacatac tgcagcttta gatcctaaaa cagctaaagc attaatgcga
     3601 ttaacaaatc agcggatcaa tgaacaaaaa ttgacttgtc tcatgatcac gcatcgaatg
     3661 gaagacgcct tgaaatacgg aaatcgtctg atcgtactcc aaaaagggaa aatcgttcaa
     3721 gatatttctg aaaaagaaaa gcaacatttg acaatgggcg accttttgca gttctttgaa
     3781 gaagtagtgg attgatttcg acagaaaaat tcctttcagt agaaggactt gaaaactcat
     3841 tgacatcttc ctttgattca tcctatactt aaaacaataa acaattaaac atatttgggt
     3901 gttttgtagg atcacgtagt caatgatttt aacaattaaa tagaatattc tttgctctga
     3961 caagtaaacg atgagaaaag aatttgctac tttgccacct agacgtttcg tactttttcg
     4021 tatcaaacgc taagtggctt tttttattga agtaagaaca aaggaggaat aagatgattt
     4081 ctggacaaac caaattagct gggctctttg ctgatccggc ttctcacagt ttatccccat
     4141 tgatgcacaa tactgctttt gaagcaaaca acatcgatgc ggtgtattta gctttccaag
     4201 taaatccaat gaatttgaag caagcagtcg aaagtattcg aacttttgat atgttaggtg
     4261 taaatctttc gatgccgaat aaaacagcgg ttattcctta tctggatgag gtaagccagg
     4321 aagcgcaatt gatcggagca gtgaatacga tcgtgcacga aaagggtcgt ttgattggct
     4381 ataatactga tggtgcgggc ttcatgcaat ccgtgcaaga agctgggatt tcgatcaaaa
     4441 agaaaaaaat gactctactg ggagctggag gtgcagcaaa ggcagtcact gttcaagcag
     4501 cgttagacgg cgctgaggaa atcgtagttt ataaacgaaa caaccaaaca ttcgctcaag
     4561 taaaagaaac attcgaacaa ctcagctatc gaacttcttg taagataaaa gttcgtgact
     4621 ttgccgatac gcatcaatta agaaaagact tgaaagaaag ctgtcttcta gtcaatgcaa
     4681 cagatatcgg gatgggaaag aaaatcaatc agtcaccgct cttagatata agcatgatgc
     4741 atgagaatct agcggttttt gatttgattt atgcacctag agaaacacgt ttattaaaag
     4801 aagcaaaaaa agtaggagca aaaggataca atggacttgg catgttgatc taccaaggag
     4861 caatcgcgtt tgaattatgg acaaaggaaa agatgccgat ccatgtgatt gaaaagctgt
     4921 ttgattaaaa aaggataata gataaagatc ttttattctc gcataaacgg tctcaagaaa
     4981 agtaatcctt tatgtatcga agcatttatt tcgttatttt ttattaaatt atcaaaaaat
     5041 gataggcttt tgtcgattgg ttatcgtatc ctttatttat cagtaagaaa cgaggaggaa
     5101 ccacaatgag tcggatggat cgctataagg acatccataa gaaggcgaaa cctctgaagc
     5161 gggatacggg gagtattttt gctagaagag aacgaaaacg aacgcgtatg gaaaatgagg
     5221 aagatgctac ccgtatttat gaaagagagc agcattcatt tgaacatttg aaccaatacg
     5281 agcaacagat atacagagat gcaccaagag cagaagaaca acaaaaaaat aaaaaatttt
     5341 tcaatcggca aaagaagaac aaaccgttaa aacctcgaag aaagcgatcg tggaaaaaaa
     5401 tcattctatc gattttgctt gttttgatcc tttattcagg tatctcattt tttattggta
     5461 aatatgtagc tgaacatgac acaagcttag aggcagcgac ttcagaaacc tttcatgggc
     5521 aaaaagcgac gaatggtgcg cataatattt tgatccttgg aagtgataca cgcggagaag
     5581 atgccgggcg agccgatacg atcatggtct tacagttgga tggtccttcc cataagccga
     5641 aactgatttc ttttatgcgt gacagcttcg ttacgatccc aggggtagga caaaataaga
     5701 tcaattcagc ttatgcatac ggaggagccg atctagtgcg tcaaacttta gtagaaaatt
     5761 ttggaatcga ttgtcaatac tatgcgaaag tcgatttcaa atcgtttgaa aaagtgatcg
     5821 atgccttatt catgaacgga gtgaagatcg atgcagaaaa agatctgaat ctggatggtg
     5881 tagatattaa aaaagggggc cagaagatgg atggacatgt tcttttgcag tacgcacgtt
     5941 tccgaatgga tgaacaagga gactttggcc gtgtcagacg ccaacagcaa gtaatgaacg
     6001 ctattttcag ccaattgaag aatccattga atctgatccg tgcaccttat gcagctggta
     6061 aagctttagg atatacatcc acagatgtca ctgcttggtt cttgacgaaa aacctctttt
     6121 cgattgcacg aggagctggt ggcattgatc gaataagtgt gccagtagac ggttcgtgga
     6181 attatgggca aagtgattat gctggcagta ttcttgaaat tgataatcaa atgaatcaat
     6241 ctgctattac agattttttg aataaatgat cataaaaaag atcaagaaga agctgcgcaa
     6301 ggcttttgtc atgaatcaat caaacgattc actcgtaaaa aacgctttct tatggtagat
     6361 ttaaccaaga gaggagctgt ttacattgga tatctatctg aacttcaaaa atgagtcgcg
     6421 tgaagccatc gctttttatg aaaaagtttt tcagactgaa gcatcaggta tcaccacata
     6481 cggtgaaatg cctggaatag aacaaatgaa cttgccagaa gaagcaaaaa atctaatcac
     6541 gaatgcacgg ctgaacattt atgatacatt agttatgttt gcagatacgc cagaaggaat
     6601 tggtcctgaa ttagttgtgg gaaataatgt ttctttagtc atccagacaa gaaatgaaga
     6661 agaaacgaag cgcctatttg aaagcttagc tgagggtggt aagattgaga tgccactgga
     6721 tgctgttccg tgggctcagt tgtatgggat ggtaactgat cgatttggca tcaactggca
     6781 gattaaccaa gagtgacagc taaagatgaa tattttctgg aaacatcaag cagcaaacgg
     6841 atcatcgttt gtttgcttga tgtttttgtg tctatttcga ctatacggta tctttttttt
     6901 attgccagat gcgaaaatat gttccataat agagaagtcc gcaaaaaatt aagtaacgga
     6961 ggtcagaaga tgaagacggt gatactagct gaaaaacctt cacaagcaaa agcctatgcc
     7021 gaaaccttcc aacagcaaaa gcggcatgaa gggtattatg aaatcagcga tccattgttt
     7081 gctggtgaag taaccatcac ttatggtttt ggacatttgg tagatatggt tgctccagga
     7141 gcttatgaag agcgttgggc aaaatggtct ttagaaaatt tgcctatttt cccagaaacc
     7201 ttccgttacg aagttccaat ggacaaacgt gcacaatttt cgatcgtaaa acgagaattg
     7261 cagtcagccg ataccatcat tattgcaaca gatggtgatc gagaaggtga agccatcgcg
     7321 tggtcgatta ttcgacaagc aaacgcattt tctaaggaaa agaactattt acgtttatgg
     7381 atcaattctc ttgaaaaaga agcaatctat gaaggtttta agaatctcca acctggaaag
     7441 aactattttc caaaatataa agaagcgcaa gcaaggcaga acgctgattg gctgattgga
     7501 atgaatggaa gtccattata tagcttgcta cttcaacgaa aagggattga tggcagtttt
     7561 tctcttggtc gtgtacaaac accgacactc tatatgattt atcagctgca agaagaaatc
     7621 aaacacttca aaaaagaaac gtactttgaa ggagaaggtt taattacgac aaacaaaggg
     7681 cagttttctg gaaaaatcgt accaaaaaaa gcatttaaaa cccaagaaga gttaacgaag
     7741 gaaatagaaa aacttggagc gcatttaggt aagcaaaacg gacggattct tgaagttacg
     7801 aaaaaggaaa agaggttgca tagtccacgt ttattttcgc tatctagttt gcagacaaag
     7861 atgaatcagc taatgaaagc aagtgccaaa gctacacttg atgctgctca aggattgtac
     7921 gaaagcaagt ttctaagcta tccaagaacg gattcctttt acattacaga aaatgagcat
     7981 cagtatctgg ttaaacatct gcaaaattat aaaaaatttt taggaataga agaagtacag
     8041 acaactgaga tcaaagcaaa gaaacgatat gtcgatgcga aaaaagttca agaacatcat
     8101 gcaattattc cgacaaagac gattccgact cctgcacgct ttgcgaagct ttccaaatta
     8161 cagcaagcaa tctatttgca agttttgcgc actactgtgg ctatgtttgc tgcagattat
     8221 atctacgaag agacgaccgt aatgacgggc gtacagcaat taaagctaaa aagcatcgga
     8281 aagatcccat tacagcaagg ttggcaggaa atcttacgca tcaagtcaaa aaagaaagaa
     8341 gctcagactc ttcctcctat cgtagaaaat gaagaagcag aagtagaact gaaaagtatg
     8401 gagaaagaaa cacaacctcc aaaaccctat accgaaggaa cgttgatcac agcaatgaaa
     8461 actgcgggaa aaacgctgga taatgatgaa gcgcaagaaa ttctcaaaga agtagaagga
     8521 atagggacag aagctacacg agccaatatc attgaaaatc ttaagcagaa gcattacatc
     8581 gaagtcaata aaaacgaaat aactgttacg ccaaaaggaa tcactctttg taaggcagta
     8641 gccaatgaac cattactgac gagcgcagaa atgactgcgc gctgggaagg gtacttgaga
     8701 aaaattggta aacaagaggg gacaccatct gtttttttag agaatatcaa aaaattcatc
     8761 ctacatttat tggcagaagt tcctaatcaa atcgacaagg tagacttttc agatgagatt
     8821 agcgggacac gtcagataaa agcgatggag aagaaaaacg atcaattagg aagatgtcca
     8881 aaatgtaaaa agggaatagt catgctttat cctaaaatcg ctacatgctt gaatccggaa
     8941 tgcgacttca aattgtggcc aacagtagct aaaaagaagc tgactaaaac gaatctcaga
     9001 gatttgcttt caaaaggaaa gacaagtaaa gttgtcaaag gttttactgg taaaaaaggg
     9061 aaattcgatg cagttttagt attaaaagaa gatatgacaa tcggtttttc ttttccatgg
     9121 atcgaaaaca gtggaacaaa agcagttgat ggaaacaaag aaacatcaag aaaaaataat
     9181 gaaaacgaag tagagtaaat gaaaacaatg aaatagcgga atatttattc cgaaaagtga
     9241 gaatataaat accaataaaa ctaaaaaata tcagtgaatc aagcaaaaac gttggggtag
     9301 gctgatttta tatagatagc aaggagtttt tttatttgaa aaaagtgcat tcatttttaa
     9361 attatcttat gggagcaatc acgattgtgg ctgtgtacca agcatttcta aagtatcctg
     9421 attacgggac gaagttgttg tcattgacgc acaatcaaac agcatattca ccaacgttga
     9481 ttttatcgac tttgactgca ggagctaaaa gcgctactta tttttttcat aaaacgacaa
     9541 gcatgctaaa gcgaaagaaa cagcgaaaac aattgttggt ggtggtagcg gctatgatca
     9601 ttatttattt tctaccagtt attacacaag tatttcatca atattttggt taagaatacg
     9661 agagtttgtg acaaaagtca tgtcacaaac ttttttcttt gtcatttttt cgtaagaaaa
     9721 agcaaaataa aagtatatac attgattaaa agtatatact tggatataat gtacttacta
     9781 tagaatgaga atggtataga gggtggcgaa atatgaaaaa aatgaaaaag tataaaatta
     9841 gaaaatctgg caatagcgat gtaacaacta tcccaccaga agtaaaagaa ttcatgggtg
     9901 tgcagacagg tgacgcaatc agttatgttt ttcaatctga tggttctgta cgaatgatca
     9961 aagcacaaga agaacctgat attgattcat tagttgattc gatcatgaat caatatgaag
    10021 acgcgttaaa agatttggtt gatttatgag gtatctttcg actaaagaaa ttatcaaaat
    10081 caacgcgaaa gttattttac gtcactcgcc aggcgaaatg atcggtgtaa aagatgcaaa
    10141 tgcattagat atggccgtca aacagccttc acaagcagta ttcaatcaag aactctatcc
    10201 tgaaatctat gaaaaagcgg cgattttagc aattaattta gctaaaaaac atccttttca
    10261 taatggcaat aaacgaactg ctcttgttgc catgttgttg tttcttcaat tgaataattg
    10321 tccagttgct ttttctcgtc aagaagctgt tgattttatc ataatgatta ccactagttc
    10381 attggatttc gattcactaa agtccaagat aaccagttat ttacgacaaa ccgcaataaa
    10441 atgaacgaat taaaaacttt atttctcaat tagatcagca tgtcattaag gaatatgctg
    10501 atttttttat gtttaatcat acaaa
//
LOCUS       DACDPS010000079        10298 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.079, whole genome shotgun sequence.
ACCESSION   DACDPS010000079 DACDPS010000000
VERSION     DACDPS010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.079"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            143..292
                     /locus_tag="IWJ07_002206"
     CDS             143..292
                     /locus_tag="IWJ07_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAP9333749.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            303..1817
                     /gene="dltA"
                     /locus_tag="IWJ07_002207"
     CDS             303..1817
                     /gene="dltA"
                     /locus_tag="IWJ07_002207"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAP9333750.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1814..3019
                     /gene="dltB"
                     /locus_tag="IWJ07_002208"
     CDS             1814..3019
                     /gene="dltB"
                     /locus_tag="IWJ07_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAP9333751.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3064..3297
                     /gene="dltC"
                     /locus_tag="IWJ07_002209"
     CDS             3064..3297
                     /gene="dltC"
                     /locus_tag="IWJ07_002209"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAP9333752.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            3300..4568
                     /gene="dltD"
                     /locus_tag="IWJ07_002210"
     CDS             3300..4568
                     /gene="dltD"
                     /locus_tag="IWJ07_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAP9333753.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4709..5791
                     /locus_tag="IWJ07_002211"
     CDS             4709..5791
                     /locus_tag="IWJ07_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAP9333754.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5791..6594
                     /locus_tag="IWJ07_002212"
     CDS             5791..6594
                     /locus_tag="IWJ07_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP9333755.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6664..7404
                     /locus_tag="IWJ07_002213"
     CDS             6664..7404
                     /locus_tag="IWJ07_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP9333756.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7415..8419
                     /locus_tag="IWJ07_002214"
     CDS             7415..8419
                     /locus_tag="IWJ07_002214"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAP9333757.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8464..10227)
                     /locus_tag="IWJ07_002215"
     CDS             complement(8464..10227)
                     /locus_tag="IWJ07_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333758.1"
                     /translation="MLFLTGIAYFFYFIFSPADSNQMKEIKDSWVFYTKDDPDFKFDS
                     KYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEES
                     NPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQV
                     LTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGFEAVSEDILSGILFEP
                     FVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAEL
                     PELMPIANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGI
                     YQTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQH
                     IQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLI
                     LARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSFRETHAIENPLYRKI
                     YLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQ
                     EKDTFIDRITVQLSRNYPNPV"
BASE COUNT         3306 a         1702 c         2083 g         3207 t
ORIGIN      
        1 aagcagtgat ttgatgtgct atttttataa aagctatgtg acaaaactgt tagtaaaaaa
       61 acggtttctg taagctagac acatgtttct ttttgtcaaa attagttaca ctttgtgtga
      121 ggttatatga ggagggaatc caatgaacaa atggcgttct aatccaaaat tgaattattg
      181 gggaaaattc atcattcgaa caatttttta tagtgcagta ctgctgttcc tgatttatct
      241 gtatcactat aaaaatattc aaggaggaac ttttatctat aacgaatttt aggaggaaga
      301 gtatggaaat caaaacgatt attgaagcaa tcgatgattg gggaataaat gaacctgatc
      361 gagttgctta tcaggcggat gagacgcata cttttggtga attgaaacaa gcatcagacg
      421 cacttgctta ttatttagaa aaaaaggtag aaggcgatgg accaattgtc gtttttggca
      481 atttggagtt tgaaatgatt gtctcatttc taggggttgt caaatctggc catgcctatt
      541 tgccgattga agagcataca cccaaggagc gtatcctatc gatctttcgg gtggctaaac
      601 catctatggt catcagtatc ggggactgga tagaagatat tccttcggtt cctgtcataa
      661 caaaaaaaga atttcaagaa atcaagaaga taccagtagg attttatccc agaaatagcg
      721 taaaaggtac agaaaatttt tatattattt ttacttctgg tactactgga gaaccaaaag
      781 gcgtacaaat cagtcatgat aatctagtca gctttgttca atggacgatg gaagactttg
      841 ggatcaagcc tggtatgcat ttcttagccc aagcgccatt ttcttttgat ttatcggtat
      901 tttctattta tcctgcatta gtttctggcg gaatgctgaa gccgttaaat aaagcagttg
      961 ttcaagactt tcgtcaatta ttcgcgacat tgccagcctt gaagttgaat gtgtgggttt
     1021 ctactccatc atttatggat atttgcttga tggaacctac attcaatgga gaaaatgtgc
     1081 cggaattgga catgttttta ttttgcggag aagaattgac gaaaaaaact gcagaaagtc
     1141 tacttgatcg attcccaaat gcacggatat ataatactta cggtccaaca gaagcaactg
     1201 tagctatttc aagtattcag attgaccagc atgtactaga tagttatgac cgactaccga
     1261 ttggctacgt aaaaaaagac acgaaagttt ctattgtcca agaaggtaaa cctgtttcaa
     1321 aaggagaaac gggtgaaatc attattgcag gaccaagcgt atcaaaaggt tacctgaaca
     1381 atcccgaaaa gacagctgct gcgttctttt gtcaaaatga cttagcaagt taccatacag
     1441 gggatgcagg tagactagat gaatatgggc ttttatttta tgaaggacgt atggatcagc
     1501 aggtcaaatt acatggatac cggattgaac tcggggatat cgaacattat ctgctccaag
     1561 acaaccgaat caaacaagct gtcgtcgtac caaaatacca aggaacaaaa gtacagcagc
     1621 tggttgcttt tgtcgtatta gaaaacaaaa cgaaacagcc ggattttcag ttgactaaat
     1681 ctatcaaaga acaattacta gaagttgtaa tggattatat gattcctcag aagttccatt
     1741 atgtcgatct gctgcctcag acagttaatg gaaaaatcga tcgaaaaaaa ttaacagctg
     1801 aggtgaatcc tgaatgatgt tacctcatat gatcccttat gcagagccgt tttatttttt
     1861 tcttttagcc attgcgcttg taccgatcgt tctatcttta ttgatatggg aaaaaagatt
     1921 accagtttat caatcagcag tgacactttt ctttttattt gtcagctttg gaggtaatta
     1981 ttggcaacag ggagcggcgc tgattgggta tgttttgtgg cagacgatac ttgtatgggg
     2041 ctattttgtt tatcggcaaa ggaaaaatca gacaggatgg ttttttgccg ccgttatttt
     2101 agcgataatt ccactgtttt taacgaaagt gactcctttt tttgaacatg gaaaagcatc
     2161 gttacttggt tttttaggaa tctcttattt aacctttaaa tctgtacaaa tgatcatgga
     2221 aataagagat ggcatgatca aaacatataa gccatttcgt tatatccagt ttctgctgtt
     2281 ctttccaaca atctcttctg gaccaatcga tcgctatagg agatttgaaa aagatcagct
     2341 ccatccgcct gttcgtgata aatatgttga tcttttggga aaaggcgtcc aaaatatttt
     2401 tgtcggtttt ttatataaat tcattatcgg ttattatttt ggtcaagtac tgcttcctgt
     2461 agtgggacgc attgccttac agcatggtgg tatttcatgg gcattagtag gttatatgta
     2521 cgtgtacagt atgtatttgt tttttgattt tgctggctat agcttatttg cagtcggtac
     2581 aagctacatg atgggatatc agacaccaat caatttcaat aaaccttttt tatcatggaa
     2641 tatcaaagaa ttctggaatc gttggcatat gacgctgtcc ttttggttcc gtgattatgt
     2701 atatatgcga ttgatgttct tcatgatgaa gaaaaaattt tttaaaagca agatcgtaat
     2761 gtcaaatatc ggttattttg ctttgtttct tctaatgggt atatggcatg gattgacgtg
     2821 gtactacatc gtatatggac tgtatcatgc ttgtctgatc tgtctgacag atgcatggat
     2881 ccgatttaag aagaagcata aagagcagtt gccttctaac aaatttacgc atggcttagc
     2941 cgtatttttg acatttcaag cagtatgttt tagcttcctg attttttcag gatttttaga
     3001 taaacttttt tttagctaac gaatagtgac atattaattt atataacaaa ggagaaaagg
     3061 aaaatggaag aacaaatttt atcaatctta gaagaaataa caggaacaga tgaagtaaga
     3121 aaaaatcgtg atattgatct atttgatgaa gggttgcttg attctttagc atctgttcaa
     3181 ttattagtcg agctagacgg agagctggat atccaagtcc ctgtatctga attcgagcga
     3241 gaggattgga gcacaccgaa taaaatcatt caacaagcaa ctgcattgaa gggttgagta
     3301 tgaaaaagaa actttttgct attttcggcc cggttgtttt agctgttggc ttactgtttg
     3361 tgttttttac ctcctcctat caagtagata tctctaatcc agaaacgatt agacgtgcct
     3421 cttcttccat gtcagtgaac gtgttgaaag gagatgcgat aaaaaattat gcattatctg
     3481 aaaaacaata tattcctttt tttggctcaa gtgaattaag cagaatcagt ccatttcatc
     3541 cgtctgttct ggcagaaaaa taccagcgaa actatcgtcc atttttgctg ggagcacccg
     3601 ggacacaatc gttaagccaa tatatgatga tgcgttctgc aggagatgct atgaaaaaca
     3661 agaaagtcgt ctttattatt tctcctcagt ggttcgttaa aaatggtgta aaaactgatt
     3721 actttaatac gtattattct gaacttcaaa catacgactg gttgttttca atgaagaaag
     3781 taacgcctgc tgatcgctat ttagctagaa gattattaac tttctcaaaa gtaaaagaga
     3841 atgatacgtt gacggcgatt ctgcaaacga tcaaaaaagg gaagctacct ttacctgaat
     3901 ctttgaatca gctgcgcagc caatggaaca tgctgaagag agaggatgaa gtattcagca
     3961 atattgggtt gaaagatcga cagcaaaaaa tcgatcatga gtcaaaacga ttaccaaaac
     4021 aataccaaga aacagaatta tccattttag caaatcaaat tggagaacga gaaacgacga
     4081 ataatccatt cgggttgaaa aacgattttt atacgcatcg tatccgagcc cacgagccag
     4141 agctcaaaca gtcacaaaaa aattgggatt atcgtttttc gccagaattt tctgattttc
     4201 agctggtctt ggatcaatta gcaaaaaacc ataacgaagt attgttcatc atcccaccgg
     4261 ttaacgagaa atggagcgat tataccggac tatctcaaga aatgttacaa ggatttgcca
     4321 aaaagatcaa atttcaatta aatagccaag gattcaatcg aatagctgat tttgtcaatc
     4381 aagcgggtac caactatttt atggaagaca cgatccatct aggctggaaa ggctggttgg
     4441 ctgcagatca acagatcaga ccgtttttag aagaaaatca tattactgct agtaagtatc
     4501 atcttgacga tgcttttttt agcaaatctt ggcaacatca aataccagat aaactacaat
     4561 taaaataaag aaaacaggcg agtgttgctc gcttgttttc tttatttttg aatattctga
     4621 ataaaagata aaaataccaa gtttgcctga acaaaaatca taagcaatgg tataatcaca
     4681 atatgaatat agaaatgggg gaaaacattt ggatacaaaa gaagaacagc tacttattga
     4741 tttaacttct gcaaaaggag tacctggaaa tgaagaagag gtaagagaag tattcagaga
     4801 atatgccaaa ccattcgctg atgatatttt ttatgacggt ttaggcagtg tgattgccaa
     4861 acatggtgct ggcggaggcc ccaaggtctt tatttctgga catatggacg aagttggttt
     4921 tatggtaaca aaaataaccg aaaaaggatt cttggaattc caaactcttg gaggttggtg
     4981 ggggcaagtc atgcttgctc agcaagttga aatcaaaaca cgtgaaggga aaatcgtcca
     5041 cggtgtgatt ggttccaaac caccacatgt tttaacacct caagtaagaa ataaacctta
     5101 tgaaataaag gatatgttca ttgatattgg cgcatcaagc caagaagaag ccaaagagtg
     5161 gggcatacgt cctggagata tggtcactcc atatattgaa tacaaacgaa tgaatggcag
     5221 taaatattta ctggcaaaag catgggataa tcgtatcggg acagctgttt ctctgcgagt
     5281 attagagaat ctatctaaag aagcacaccc aaatgtttta ttcgcaggaa gcgatgtgca
     5341 agaagaagta ggcttgcgag gtgctcgaac aagtacacac cttgtaaatc ccgatattgc
     5401 ttttgcttta gatactggta cagcaggaga cactccagga atgacaccaa aagaagcaga
     5461 ttcgatttta ggaaaaggcc cacagatttt gatttttgat gcatctatga tcccgcataa
     5521 aaaattattg aattttgtta ttgatgtagc agaggaaatg gatattccat tccaatatac
     5581 agtgatttct ggcggaggaa ctgatgcagg acaaatgcat ttgacaagag atggtgttcc
     5641 gtctgtagcc attaccgttc ctgttcgtta tctgcattcc catacatcag tgattcatga
     5701 agatgattat ttgaatacag tgaaactagt cacagaatta gtgaaacggc ttgatagtga
     5761 aacagttgaa aaaattcgga gttatgaata atgaataata gaatacctat ttctgaatat
     5821 cggaaaaaag cacgagtttc tttggaaaat caatggggaa tcaatacttg gcttgttttc
     5881 ttatctataa tatttacgag cgtcatccag aacttttttg acggtttcag tccttttccg
     5941 gaagaaagca tgcagcagca cttattgagt tttctagtcg ataatttact tttattcgca
     6001 ttcaactatg cattgtttta cattgcgcta gtagttgtac ggggtggcag accaaaaagt
     6061 aaccttttat tttcagtttt ccaaagaaag tattttggtt cgatacttgg catcaatttg
     6121 atcaattcag tcatcaactg gctactcaac agtttaatct ttctgcccat gtttttaatc
     6181 agcggagtga gtacttatat gcagctgctt ttcagttcaa atgcggtacc tacgggtgtc
     6241 atgccagaag attttcttga tatcagcttt gttttatcga tgatagggat gatttttgtt
     6301 tcagcactca tcatgagtat tgttggcggt ttattccaat ttgctgcatg gacaaagttc
     6361 gactatcctg atttgtcggt tattcaatgt ttgaaatatg gctggttttt attgaaagat
     6421 agaattggta cgtatatttt gttgcagctg tcttttatcg gctggtattt gctaggagca
     6481 atcgctttgt tcatcggtct tttgtgggtc attgtttatg tgaatgtgtc agttgcagaa
     6541 ttttatgaac aagcacggat tgaaaaagga ggtccagacg cctattttac ttagcatgta
     6601 agcagtttat tcatcaaaaa aataactttt gtggtaggat aaatctttga gggggaatgt
     6661 acgatgaaat taactgtttt aggatgctta ggagcgtatc cttataaaaa tcaagggaca
     6721 acaggttatt tgcttcaatc tgacgggttc aatttgttga tcgatgcagg tagtgctacg
     6781 ctgatcaaat tacaagaaca ccttgatcca cttgatctag atgctgtgat ccttagccat
     6841 tatcatcatg atcatattgc tgaccttggt gtattacagt attactggca acttcatcct
     6901 aagagaaatc cggagacatt gctttcaatt tatggacaca caaaagattc cattcacttt
     6961 gaagaattga cgttggaaaa tgtctcaaaa ggacatcctt attttgaagc tgaagaatta
     7021 aaaattggtc cttttctgat tacttttatg gaaacaatcc atccagtagt atgttacgcc
     7081 atgcgtatcg tagaagaaac aacggggaaa gtattcgtat ttacaggtga ttcaggttat
     7141 ttggaaagtt tcgctgattt tgcgaaagat gctgatttgt ttctagcaga tacctatctt
     7201 tttgaaggaa atgaacatca tcatgcccat ttcacttcaa aagaagctgg agaacttgcg
     7261 aaatcagcta acgtaaagaa acttgtactt acacacctac ctcagtttgg tgatttagaa
     7321 caattgaaaa atgaagcgca aaaggcagca ggcaaaggga ttgatataga acttgctgca
     7381 gttgacaaag tgtttaacat ttaggaggaa gaacatgatt tttgtaccaa atgaaaacca
     7441 cgacccaaga atcaatttag caattgaaca atatttgctg caagaaatgc ctttagatga
     7501 acctattttg ctcttttata tcaatgaacc ttcaatcatc atcggcagaa accaaaatac
     7561 gcttgaagaa atcaatcgag attatgtgga agaaaatggc attcatgttg tccgtcgctt
     7621 aagtggaggc ggagctgtct accatgattt tggaaacctg aactttagtt ttatcatgcc
     7681 tgatgatggg gattcattcc gtgactttgc gaaagtaaca cagccaatca tccaagcact
     7741 tcatgaattg ggtgttgctg gggcggaatt gaaaggccga aatgatctag tcattgatgg
     7801 catgaaattt tctggcaacg cgatgtatgc taccaacggt cggatgtttg ctcatggaac
     7861 aattatgttc gactctgata tcaatgaagt agtcaatgca ttaaaagtaa aaaaagacaa
     7921 aatcgaatca aaaggaatca aatcgatccg ttctcgtgta acgaatatca aaccatttct
     7981 tccagaagaa aagcaaaaaa tgacaacaga agaatttcgt caagaaatct tgttgaaaat
     8041 tttcggcgta gagtcagtag aagaagtaaa aacgtacgaa ttgactgatg aagattggaa
     8101 aaaaatcaat cgaatctctg aggaatatta tcgaaattgg gattggaatt acgggaagtc
     8161 tccagatttc aatttctctc gccaaaaacg tttttctatc ggttcgattg aagtccattt
     8221 gaatgtctca gaaggaatga tcaaagaagc aaaaatcttt ggcgatttct tcggactagg
     8281 agatatctct gacgtggaaa aaaaattggt tggacagaaa tatgataaag catctctatc
     8341 aaaaatagtc agccaaattg atgtgaagaa atattttggt gcgatagaac cagaagaatt
     8401 gcttacctta ctttattgaa aataaaaaat agcggaatcc acatatatgg attccgctat
     8461 tttttataca ggattcgggt aatttcgaga gagttgaaca gtgatccgat caatgaaagt
     8521 atctttttcc tgcgttagtg ttcctgtgta acgatccgct acgtctttca tcatcattaa
     8581 gtcaaaacgt tcctgatcat ctaattcttc tgtgatgcct cgactaaata ggtttgtatc
     8641 gtaatgtgca ctatgttcgc aacaaatgac gaaatgttcc tcttgttcgt gtatcgacaa
     8701 ataaattttt cgatacagtg gattttcgat ggcatgagtt tctctaaagg aatgctctaa
     8761 agcgtggatc aaaagttgag ccagatcgtc atcgttgata ggaagttctt cgggaagatc
     8821 tgcttggaag gttatctgaa tattccgttt ggcagctaac ttctggtaac gcgctaagat
     8881 aagatttgtc acctggtggt tgcaaaaaga acacagcatt tcgaaatttt ttgcttcttc
     8941 taatatcttt tccacatagt ttttagcttt tgtttggtcc gtatgcatta aatcttgcag
     9001 attctccatc tcttggacaa attccaggcg aagtgtattt acttcttgga tatgctgtcg
     9061 aagctgctca taatgttgct ccaacaaatc atttttgatt tttaagaaat ctacttgtct
     9121 ccgatctttg tcggtattta agatgtgatc gattagattg tagctgataa taacgattag
     9181 aatgataata tataaaatcg tagaccaatt gatcgttgtt tgatagatac ctaatgccgt
     9241 ttggatgtaa atcaagcaat ggataatggc taccaagacg atccaaggtg tacaagctac
     9301 ataaaaacgg ttattcttat atgcttcgcc aatgcatgca agtgaagtga ctaaagtgct
     9361 tataacacta gcaatattag caatcggcat taattcagga agttctgcag gagtaaaagt
     9421 tgcataaagc agaacgccga taaaaatcaa aagttggaac gtacaaaaaa ttccgtacca
     9481 ttttttatag taaaacattc ggctccaata gtagcagatg atgaaagctg acgttaagat
     9541 aataaataaa tgagataaaa aagaatgaac gaatggttca aaaagaatac cacttaaaat
     9601 atcttctgat acagcttcaa atcctaatgc caaagcaaag caacttaaga gaataagtga
     9661 gtattcaaga cgtttttgtt tatacatcaa gtagatcgta tagaaaaagg tccctatagc
     9721 gataaaaaca gcgattatca tcgtcaatac ttgcggcact gattgtgaat aaataaaact
     9781 aatcaatggt ccagttgttc ctagataaac tttcggtggc agtccagcat agttctgata
     9841 aggagatgag actgtaaggg tcaatttttt tccgatataa tttgctggta aatctatcac
     9901 agccaatgag agtccgggat tgctttcttc ctgataatct ttccgttggt ataaaatctt
     9961 ttcgcctaga tatacagtaa tccattgatg gtttcctttg attagtaaat ttgcttcgtc
    10021 caacggtttt tcaagtgtcg tttccataat gaatgtttcg tctttatcca cggtaggaat
    10081 cgtacgagtg tacttgctgt caaacttaaa atcaggatca tcttttgtat agaaaaccca
    10141 cgaatctttt atttctttca tttgattgct gtccgcagga gaaaaaataa aataaaagaa
    10201 ataagcaatt cctgttaaaa ataacaatga taatataatg attatgctcc actttatacg
    10261 taaataaaaa gattcttttt tattatatat gttattaa
//
LOCUS       DACDPS010000080        10207 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.080, whole genome shotgun sequence.
ACCESSION   DACDPS010000080 DACDPS010000000
VERSION     DACDPS010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.080"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(885..1550)
                     /locus_tag="IWJ07_002216"
     CDS             complement(885..1550)
                     /locus_tag="IWJ07_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9333759.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            1676..3712
                     /locus_tag="IWJ07_002217"
     CDS             1676..3712
                     /locus_tag="IWJ07_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAP9333760.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            3716..4354
                     /locus_tag="IWJ07_002218"
     CDS             3716..4354
                     /locus_tag="IWJ07_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9333761.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4382..4810
                     /locus_tag="IWJ07_002219"
     CDS             4382..4810
                     /locus_tag="IWJ07_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333762.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            5267..6178
                     /locus_tag="IWJ07_002220"
     CDS             5267..6178
                     /locus_tag="IWJ07_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9333763.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            6632..6946
                     /locus_tag="IWJ07_002221"
     CDS             6632..6946
                     /locus_tag="IWJ07_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9333764.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            6962..7348
                     /locus_tag="IWJ07_002222"
     CDS             6962..7348
                     /locus_tag="IWJ07_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9333765.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            7377..8762
                     /locus_tag="IWJ07_002223"
     CDS             7377..8762
                     /locus_tag="IWJ07_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP9333766.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            8765..8917
                     /locus_tag="IWJ07_002224"
     CDS             8765..8917
                     /locus_tag="IWJ07_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9333767.1"
                     /translation="MLAGVAIATPALNPPYLTGGYKSTGNSQKNIRKSFGYKGFTKFQ
                     RMSNGL"
     gene            8940..10145
                     /locus_tag="IWJ07_002225"
     CDS             8940..10145
                     /locus_tag="IWJ07_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP9333768.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
BASE COUNT         3472 a         1671 c         1992 g         3072 t
ORIGIN      
        1 gacaaagagt tggcaaaaga tgttgataag gaagataaga taactgtgtt atataaacta
       61 ggtaactaac tgtaacaagc taattttaag agattcttat tagaagacgt ttaggaatat
      121 aactaagaaa atgattatac aggtaattgt catttcaata atgttttcta attttcgcgt
      181 ctttgtttta agaaaaaggc cagaatcagt tggaaaaaag ttcgtacgat agtgaagagg
      241 ggcaaaattt cctactaata ttcccactaa caagagataa aacggccagg ttggtgtgat
      301 tcctctataa aaaaatgccc atgctaccga aatcccatag attaacaact gaacaatttt
      361 tacaatgaaa aaattgttct gccattactc aaagtctctt cctccttgta tcgtttattt
      421 gtttcatttt atcattatga taggcgcttg aacaatccat tttgtatttt attgagtact
      481 cttggataaa ctaccagcga ttatcatatt tatttatgtt actgtaagcc atcatataaa
      541 agtaaatgag cagatcatag aaatataaaa aagaactaaa attgatctaa attatgtgca
      601 gcttgactac gtaccaatct atattactta ctagattggt cgttaatcta ttcggatcga
      661 atgagcaaaa tatttttgtg aatgtccatc cttgattatg attccaaaaa gaatcattgg
      721 gaggctgtgg cgtaaatatt atactataag ccagaaatac ctatcattct gaactttttt
      781 atttaggaac tttcatctca aggctttttc aattaatttg tacccctgtt ttttggataa
      841 gatcatgtgc attgattttt tctatctcta cgttcatctc ctacttaatg cctatctggc
      901 tcctgagggc ttctttttaa ggcgtcgtca tagtcttggc tgaggatttc ttggtaatct
      961 gagggaatgg cttcccaagt gagcaggcga acgttcatga cgtgttttcc tttgtgtttg
     1021 attacagcat ggtttttctc tttttctacc tctccagcgt gaaaagacag ccatgtacca
     1081 tggccaaaga tatcattaca tacccaacgt tcgatggaat tgtccacgta tcctacaggg
     1141 agtgtggtat agccgatttg tggtttcaaa aaaggattga atcgatcgat cgtttcattt
     1201 ttgtattgat aattgataag aaaaagatag accaaacaaa agacgagcag aaagaaagat
     1261 agtgtctttt tgagtgagaa gggttgtttt gatgaataag atttcttgtt aacaagaggt
     1321 tcatctggca atgttggttc tattgattcg gtagctgatt caatagaagg aaaatcaatc
     1381 ggcagaccta aagtatgatg taaaagtgac agcatttcaa ggtctggcat tgtacgagaa
     1441 ttttcccaac tagaaatcgt tttatcggaa acaccaattt tttcgcctaa ttctttttgc
     1501 gtaagcttac aatttctgcg gtaagttcgt attttttctc cggtgttcat cttcttctcc
     1561 tttcgattct acagtatata gatttattct acatgaccta tacacttgat agatacgttt
     1621 ttttgatgaa aaacggtatg attttatcaa agaatcattt aagtaaggga gagtggtgat
     1681 aatcaaaaaa aagttcacag ttttactcac aatcatagtg accggtatca tgagtggttt
     1741 tgccttggtt agtataaata aacaggtact ctatcggaca aatgaatttt tccatcaaac
     1801 agatacttat actccgattc gattgccaca acattttatt gggcaagaag aatatgagca
     1861 gctaatcatg gtatttgaaa atgtctcatc agaaactgag ctttcttata tgaaaaaaac
     1921 agtaaatgaa tccaatgaag tagatacatc ttggaacttc aagatacagc caaagcttga
     1981 aatgacattg tattatcaag gggatgtcaa atcttctcac gagaaggtac cctattctaa
     2041 tacctccttt cgcgtaacgt ccattgaaga atcgatcaaa ataaaagatt ttgaaggtga
     2101 atttttcttg atgacgagag aaaaagaagc ctatgaacgg ttcatttcac tattcactga
     2161 tcgattcaat caagcatttc acactgatta tgccactaat atttttgaag actttgaaaa
     2221 tagctattcg tattcggtta tttcaaatac agacgtatat aattatcgag tgtatttaac
     2281 tattggattg attcttttat tccttttgtt atcatccttt ttcttattga tgaataaaga
     2341 aatcagttta ttgttgaata atgacttttc attacatcaa gtattgtggt gtttattggg
     2401 gaaaaagaaa atcattatta cattcataat tgcaggaatc attggtggtt ttatttttat
     2461 gtattataag gagcaatttc tttatttgtt ccagatgtac gttttcttgt tcagcggaat
     2521 atatggtatc tctttgattt ctcttttatt agttgaaaaa atcgcttcgt taagagaagc
     2581 gttcaactat atccaataca ttctttttac atttggattg ttgttttctc ttcttttgac
     2641 cgttgccaat gtcgaattag caactatcgt tttttcttct tcacagttag ctgtttccca
     2701 tgaaccacca aaagaaatcg ctggtgagga ctatcatgta ttttatccag taaccatcgg
     2761 gaaaaaccac gtggatttta tttattccaa tcgttttgca tcagcagcag atcaggaatt
     2821 atatgaaact ttgaataaaa acggaagtat attagtcaat gtatcagatt acttgaatga
     2881 agaaaatgaa cagtttggca gagggataaa aataaatctg aattatctta agaaatttcc
     2941 tttgattgat gtctctggaa ggttgatcca aataaccgaa aaagaaacgc accctgttat
     3001 attgatccct gaacgatata gacatacaga tttaaaaaat gacctggcgc aaataacaga
     3061 atattatcaa gaaatcagtg aaaaagaacc gaaatttcta tttataaaaa acaatcaacc
     3121 catttataca tttattcctt ctatcccctg gatcgaacat tatccggttg ttgaaatact
     3181 gacattgaaa aacagtatgt atttggaaag aaatattctc agcggagaaa tctatccacc
     3241 gttaaagatc aagataggct ctttgtcgaa agaaaggtta tttgagctga ttgaagaaag
     3301 tcagttacgt gataacttgc agttatcatt tccgtatact cagactgaag agatagctgt
     3361 caaagtgtta agcagatcat ttcattactt gatacagata tttttactca ctttcttttc
     3421 tttcttcttg ttgagctatg tcatgttatg catatatttt gtctacaatt gtcggaaaat
     3481 cgctatcttt cgttcaagtg gttattcgct ctttgaaacg tataaagatt tcttcatgat
     3541 gaacctcatc aaatggggaa cgacaagtgt aatcttcctt tttcttatag aaagagagcc
     3601 taaatatctt tttaatattt ttttctttag cttcattgat tttattcttt cagttgtttt
     3661 tattttgtct acagaaaaga aaagtcagtt attgctgatg aatggaggtt aacaattgga
     3721 gccgttgata gtgataaaag gtgcgaaaaa aatcattcga aacaaaacga ttcttcattt
     3781 ggatcgtttc actgcggatg ctggggatct tgtgactgtt gttggagaaa gtggagcagg
     3841 taaaagtacg ttattgaata tattaggatt gaatgatcag ttcagttcag gacaattcta
     3901 tttgttcgat caagagacga aacaattaac tgggagagag aagcgcttga taaaaagaaa
     3961 taccatatct tttctttttc aagattttgg gttgattgaa tatgaaacaa tcgattttaa
     4021 tttagatatt ggattgaagt tttcgaattt aaatgtttca gataagcgaa cagcaaagta
     4081 tgaggcattg aaaaaagtaa atataaacaa gaagctaagt acgcctatct acacattatc
     4141 tggtggtgag aagcaaagaa tcgcctgtgc gagagtctta ttgaagaata gcaaaatcat
     4201 tttagccgat gaaccgactg ggtcattgga taaaagaaat aaggataaag tagttgaaat
     4261 cttatcgcaa caagcttccc aaggtacgtt agttatcgta gtcacgcatg atccatatct
     4321 gaccaaaata ggaaacaaga agatcatgct ttaaaaatca aaaaggaaag gatgtcgttt
     4381 tatgaaaaaa gcagttatta tttgtgcact agggataggt tttgttggag gaaacgctat
     4441 gtcatctgaa gtggaagcaa attcagttca cggtgaaatc tattacaaat ctttaaagcc
     4501 aggagagatt cattttgatg ctaaaggtaa tattattaag tcaaatttac cagtagaccg
     4561 ggaaatttct agaacagcaa ttaatcatag ctctggaaga tgggtttatt atagtgatac
     4621 aacttgtacg agaaaaacag gtcattctaa ccactatagt cgtgtctata atcgtcacgg
     4681 tgcaaaagcg aaagttggaa actctattcg atcagcaaat gcaacaaaag gagtatgggc
     4741 ctatgcaagt gcgacaggaa atagaactga cacattctca tgttggtaca atccaaccgg
     4801 ttggtattaa gtatccaaaa gtttatataa aatagaaaaa acatataatg aaacagcgtt
     4861 aaaagttatt tttggtagtt ttaatatatt catttttagt tgtataaaaa tatatcataa
     4921 taaaaacaac aaaaaaatgt ctggaaaatc gaacataaaa gtgtctgatt tgaatatccc
     4981 agcagcggaa gaagaaaaac aaaatcgata acatttgttc taagaattag ttacgttaaa
     5041 tacggaacgc aagatagact tgaaaaacgg taacaaaaga agtagtatat taaggtgtga
     5101 tgtgctactt gacatttatt ctgctgagcg aactttctgg attgtgtagc cttatatcta
     5161 ttcaggacgt atcgagcaga ttagtaaagg tgttcttgat gattttcatc ttgaacacct
     5221 ttgtttttag caattcgcaa aaaacgaaaa gtaggagaat tgataaatga aaaaaagatt
     5281 tttagcactt gccatcgtac taggcactgg cttattgtca ggctgtacga atgccggtga
     5341 aaaaacagcc gttagttata aaggcggtac aatcagcgaa caagaagtaa tggatagctt
     5401 gaaaaaaatg caaggagcag attccgctgt gcaacagttg atcgtttatc aagtatttga
     5461 agataaatat ggagatgacg tatcaaccaa agaaatcgat tctcaatatg atcaaacaaa
     5521 gaaacaattg ggtgattcat ttgattcaca attgaaatct gcaggttaca cagaacaaac
     5581 attcaaagac agtatcaaac aaagtctagc ctttcaagaa ggtctaaaga aacatatcaa
     5641 attgaccgat gaagatttaa aaacggcttg ggaatctttc catccagaag tggaagctca
     5701 aattatccaa gtagcaagtg aagatgatgc aaaagatgtg aagaaagctg ctgataaggg
     5761 agacgatttc tctaaattag ccaaagacaa gtcaactgat actacgacaa aagaagatgg
     5821 cggaaaagtg aaatttgatt ccactactac aacggttccg gcagaagtca aagaagcagc
     5881 ctttaagttg aaagatggtc aagtttctga tgtgatcact tctaccaatg cttcaactta
     5941 tactacggaa tattatgtgg ttaaaatggt gaaaaaccaa aacaaaggca acgacatgga
     6001 taaatacaaa aaagaattga aagaaattgc aacagacaca aaattaagtg acagtacctt
     6061 ccagaataaa gtaattggcg aagtattgaa agacgctaat gtaaaaatca aagacaaaga
     6121 ctttgaaaat gttttgagta cattcacttc agattctagt acagcaagta gtaaataagc
     6181 ggtataaata aaaaatatga actatttaaa tcaataaaac tgttcatcta aatcttttta
     6241 ttagaactaa atcaacagta gtttaattac tacttgattt agttcttttt ctacaaaaaa
     6301 tagcataaaa atctagttat ccgcataaaa actggactta tcacacttta tcaaggtcaa
     6361 aaccactcaa tttactacta atttactact tatgaatgag ctttgatacg acgatttatc
     6421 cttgaaaagt gaagatataa agatacttcc aataaaattt gaatatttaa taggtagaca
     6481 cttcaaaaaa tgaggtgtct atttttttac ccgattttga aaggaagtga acttatgaaa
     6541 acaaaaaatc aagaatcaaa aggtcgttcc ccactcttta agaccatcaa acattcattc
     6601 agccaataaa aaagaaagga taggtaaaaa tatggaactt aaatttgtga ttcccaacat
     6661 ggaaaaaaca ttcggcaatt tagaatttgc tggcgaggat aaagtcgttc agcgaagaat
     6721 caacggacgg ctaactgtct tatcaagaag ctataatctc tattctgatg ttcaaagagc
     6781 agatgatatt gtggtggtgc ttcctgctga agctggcgaa aaacatttcg gctttgagga
     6841 acgtgtgaag ttagtcaatc cacgtattac cgcagagggc tacaaaatcg gcactcgtgg
     6901 ttttacaaat taccttttac atgctgacga catgataaaa gaataaagaa agagaggaaa
     6961 aatgatgaga ttagcaaatg gcattgtatt agataaagac acgacttttg gagaattgaa
     7021 attctctgct ctacgtcgtg aagtgagaat ccaaaatgaa gacgggtcgg tttcagatga
     7081 aatcaaggaa cgtacctatg acttaaaatc caaaggacaa ggacgcatga ttcaagtaag
     7141 tattcctgcc agcgtgcctt tgaaagagtt tgattataac gcacgggtgg aacttatcaa
     7201 tcccattgcg gacaccgttg ctactgccac ctatcaagga gcagatgttg actggtatat
     7261 caaggcagac gatattgtgc tgacaaagga ttctagttca ttcaaagctc aaccacaagc
     7321 aaagaaagaa ccgacacaag acaaatagtc gctaggtaga aaggagactt tttcgcatga
     7381 aacagcgtgg taaaaggatt cgcccatctg gtaaagattt agtctttcat tttacgatag
     7441 cgtcactcct gcctgttttc ctgctggttg tcggactgtt tcatgtgaag acaatccagc
     7501 agatcaactg gcaggatttt aacctatcac aagcagataa gattgacatt ccctatttaa
     7561 ttatcagttt cagtgtcgca attcttatct gcttgctggt agcgtttgta ttcaaacggg
     7621 ttcgctatga tacggttaaa caactttacc accgtcaaaa actggcaaag atgatacttg
     7681 aaaacaagtg gtatgaatct gaacaggtca aaacagaggg tttctttaaa gatagtgctg
     7741 gtcgtacaaa ggaaaagata acctacttcc ctaaaatgta ttatcgactt aaaaatggct
     7801 tgatacagat acgggtggaa atcacgctgg gaaaatatca agaccaactc ttacacttgg
     7861 aaaagaaatt agagagtggc ttgtactgtg agctgacgga taaagagtta aaggattcct
     7921 atgtggaata tactttgctc tatgacacca tagccagtcg tatttctatt gatgaagtag
     7981 aagctaaaga tggtaaactt cgcttaatga aaaacgtatg gtgggaatat gataagctcc
     8041 ctcatatgtt gattgctggt ggtacaggtg gcggtaaaac ttactttata ctgacactga
     8101 ttgaagcctt gcttcataca gattcaaaac tgtatattct tgacccgaaa aatgctgacc
     8161 ttgcggactt aggttctgtg atggcaaatg tctactatag aaaagaagac ttgctttctt
     8221 gcattgaaac attctatgaa gaaatgatga aacgtagtga ggaaatgaag cagatgaaga
     8281 actataagac tggcaaaaat tatgcttact taggtctccc ggcacacttc ttaatctttg
     8341 atgaatacgt cgctttcatg gaaatgctgg gaacaaaaga aaacaccgca gttatgaata
     8401 agctgaaaca gattgtcatg ttaggtcgtc aagctggctt ctttctaata ctggcttgtc
     8461 aacgtccaga cgcaaaatat ttaggcgacg gaatccgtga tcagtttaat ttcagagtgg
     8521 ctttaggtcg tatgtctgaa atgggctatg gcatgatgtt tggcagtgac gtacaaaagg
     8581 atttcttctt aaagcgaatc aaaggtcgtg gctatgttga tgtaggaaca agtgtcatat
     8641 cagagtttta tactcccctt gtaccaaaag gatatgattt cttggaggaa attaaaaagt
     8701 tatccaacag cagacagtcc acgcaggcga cgtgcgaagc ggaagtcgca ggtgtggact
     8761 gatcttgctg gctggtgtgg caatagccac gccagcactt aaccccccgt atctaacagg
     8821 ggggtacaaa tcgacaggaa acagtcaaaa aaacattaga aaatcctttg gttacaaggg
     8881 atttacaaaa tttcagcgta tgtcaaatgg gctttaaaag ttgacatacg cctttttgat
     8941 tggagggatt tttactgaat gaacaaactt ggttacagca tttaaaagaa aaacgcttgg
     9001 cttatggact atctcaaaac cgtttagctg ttgcgactgg tattacaagg cagtatctaa
     9061 gcgatattga aacaggaaaa gtcaagccat cagaggattt acagcagtcc ctttgggaag
     9121 ctctggaacg cttcaatccc gacgctcccc ttgaaatgct gtttgattat gtaaggattc
     9181 gctttccgac aacagacgta cagcaggtgg tcgaaaacat cttacaactg aaactgtcct
     9241 attttcttca tgaggactat ggtttctatt cttattcaga gcattatgct ttaggcgaca
     9301 tattcgtcct ttgctcccat gaactggaca aaggagttct ggtggaattg aaaggtcgtg
     9361 ggtgcagaca atttgaaagc tatcttctgg cacaacaaag aagctggtat gagttcttta
     9421 tggacgtttt ggtggctggc ggtgtgatga aacgccttga ccttgccatt aacgataaga
     9481 cagggatttt gaatatccct gtactcactg aaaagtgcca acaggaagaa tgtatctccg
     9541 tcttccgcag ttttaaaagc tatcgcagtg gcgaactggt acgcaaagag gaaaaggaat
     9601 gtatgggaaa caccctctat atcggttcat tacaaagtga agtttatttc tgtatctatg
     9661 aaaaggacta cgagcagtac aagaaaaatg atattcccat tgaagacgca gaagtaaaaa
     9721 accgttttga gattcgattg aaaaatgagc gtgcctatta tgcagtccgt gatttactcg
     9781 tctatgacaa tccagagcat accgccttta aaattatcaa tcggtatatc cgttttgtag
     9841 ataaagacga ttccaaacct cgttctgatt ggaaactgaa tgaagaatgg gcttggttta
     9901 ttgggaacaa tcgtgaacga ttaaaactaa ccacaaaacc agagccttac tccttccaaa
     9961 ggacgctgaa ctggctatct catcaagttg ccccgacctt aaaggttgcg attaaacttg
    10021 atgaaatcaa ccagacgcag gttgtaaaag acattctcga ccatgcgaaa ctgacagacc
    10081 gacacaagca gattttgaag caacagtcag taaaagaaca ggacgtgata acaacaaaaa
    10141 aataactcaa atacaaattc attgaatata gagaggagaa catttttatg aattttggac
    10201 aaaacct
//
LOCUS       DACDPS010000081        10128 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.081, whole genome shotgun sequence.
ACCESSION   DACDPS010000081 DACDPS010000000
VERSION     DACDPS010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.081"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            117..623
                     /locus_tag="IWJ07_002226"
     CDS             117..623
                     /locus_tag="IWJ07_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAP9333769.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            640..1539
                     /locus_tag="IWJ07_002227"
     CDS             640..1539
                     /locus_tag="IWJ07_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAP9333770.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1626..2510
                     /locus_tag="IWJ07_002228"
     CDS             1626..2510
                     /locus_tag="IWJ07_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP9333771.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(2702..2773)
                     /locus_tag="IWJ07_002229"
     tRNA            complement(2702..2773)
                     /locus_tag="IWJ07_002229"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2738..2740),aa:Arg,seq:ccg)
     gene            2833..4167
                     /gene="rpoN"
                     /locus_tag="IWJ07_002230"
     CDS             2833..4167
                     /gene="rpoN"
                     /locus_tag="IWJ07_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAP9333772.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            4370..5407
                     /locus_tag="IWJ07_002231"
     CDS             4370..5407
                     /locus_tag="IWJ07_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAP9333773.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            5448..6449
                     /gene="gap"
                     /locus_tag="IWJ07_002232"
     CDS             5448..6449
                     /gene="gap"
                     /locus_tag="IWJ07_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP9333774.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            6559..7749
                     /locus_tag="IWJ07_002233"
     CDS             6559..7749
                     /locus_tag="IWJ07_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAP9333775.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            7853..8608
                     /locus_tag="IWJ07_002234"
     CDS             7853..8608
                     /locus_tag="IWJ07_002234"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAP9333776.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            8724..10022
                     /gene="eno"
                     /locus_tag="IWJ07_002235"
     CDS             8724..10022
                     /gene="eno"
                     /locus_tag="IWJ07_002235"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAP9333777.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT         3548 a         1869 c         2099 g         2612 t
ORIGIN      
        1 tgtcacacct tcttttttgt atataaaaat cccaagtaaa tacataaaaa ataaaaaaat
       61 tgaaactttt agcgcacctt gtacgtcata ttaagtgagg cagaaaggag tggagaatga
      121 tttttactgg atataagaaa aaacggcaga tcgaaaaata ccgtcacctg ctgaatggac
      181 tcatcgaaac tgattatcaa aaaatgttcc gtatcacgat gaattacgta catgataaag
      241 aagaggcctt agatgtgatg caagatagtt ttcataaagc cctgaactat tttgaaaaag
      301 aaaaagagat cgagcatttt agtgcatggt tttatcggat attgatccgc acggcattag
      361 atagttggcg cagacaaaaa aggacgcctg tggacttatt tgaaattcaa gatatccccc
      421 aacagttacc tttagaaact tccgttgcag agctccatat gattttagat aaaatagaca
      481 gtccggaaaa agagattctg atcctctact tttttgaagg atttcgtttg aaagaaattg
      541 cgactatctt ggatatgaat gaaaacacag ttaaaacgaa aatgtaccgc tcattgaatg
      601 ctttacgtca aatcctagaa tgaaggagag aagagtgcca tgtattccca aaaagaaaat
      661 ttaaaaaaac taaagaaaac ttataaagaa caagcaattc ccaaagggct taaagaggag
      721 atccgccagc gttttattat cgaagagcaa tcatttataa aagcaaagcg gagaaagaga
      781 cgtctccaag ggttcggagt atctttggct atcgtaatga ttacaacaag tagccttctt
      841 tttaatagtc aagttcgaag ctttgcagaa gatttaccca tcttaggatc agtcattgaa
      901 ttgatcttag gcgaacgctt tactgatcga tctgaaaaaa tcgatattca ggtaccgaag
      961 atcaatacac aaaatgagaa agaaaataaa acaattcatg gattaaatca aaaatacttt
     1021 cgcgaaggac aagcagattt tgaaaaagct gaaaaagaat atggaaattt tgaaacggat
     1081 cattaccaag ttttgggaga ttatcagaaa atcgtagacg ataaccggtt tctcgtgatt
     1141 gaaaggcaga tcacccaaac agcagcagat agccatgttg aaaaaagata cgacacaatc
     1201 gacaagaaaa acagtgtcca gctctcactt ccacttttat tcaaagatga cacctatcta
     1261 tctgttttga caaaagaagt caaacggcag atggccgaac aagtcaagga agatccatct
     1321 aagtactatt ggactgagca agatattcaa gaagggacga tagaaaaacc aacacttgtt
     1381 acgcctacac gaagttttta tttgaacaaa gagcatcaat tagttttgac tttttctcaa
     1441 tacgaaattg ctcctggtta tatgggtaca ccagaatttg tcattcctaa gtccgtgaca
     1501 aaaacgattt tggcatcgga agattatttg gatcattaga cgagaagtag ttgcgactag
     1561 cagtttcaat tttcaagtaa gtacaagtaa ataataagaa gagaaaaaga gaaagaagag
     1621 atatagtgaa aagaaattat ttagttatca gtattattgc tttatccgtg cttttgtttt
     1681 tcgaaattac ttcggtggcc tccttgatca ccgaagtaaa aggacaaaca cactcttcgg
     1741 agcattcgtc acaatctttc aaacaatcag aacaatcaac cattaaaagc caatcaactg
     1801 aaacagctaa cgtcaaagaa gccattcaag aatcagcgga aaaggtacaa agtcagtcat
     1861 cacaatcaac acaagacagt acaaaaacga gccgagatcc ggctgtcaaa agcgtcgcaa
     1921 tcagctttga tgatggacca ggagcgacca caacaccgca gcttctgcga atcttaaaag
     1981 aaaaaaacgt gcatgccact tttttcgtct taggagaaaa tacggcgcag catcctgaaa
     2041 tcgtgaagca gacagctgaa gcagggcacg agattggcaa tcacacatac gatcatcaga
     2101 atctggccac tctttctgcc cagtcgatga cggaggaagt gacgaaagca gacactgaga
     2161 tcaaaaaagt cactggcaaa acccccactt ttgttcgtcc gccctatggt tctgtcacga
     2221 atgtaggagc aacgatcatc cagcgaccga tcatcgaatg gtctgttgat tcagaagatt
     2281 ggaagacaag aaatcctgat ttgattttgc agaagatcca agcaacagta tacgatgggg
     2341 caattatttt attccatgat atctatcctg aaacgattcg tgccgttcct caagtgatcg
     2401 attatctgaa agagcaaggg tatcgcatca cgacagttgg ggatttatta gggcatccaa
     2461 cggctgtcga gaactattat ggcagaaacg atcatcggcc agttcagtaa gtgagacaag
     2521 tatctttaat aaataacaca ttttaataag tagttgaaca cataactacc ttacttgcaa
     2581 aggcttaaca atatctatac aggtacaaca aaaaactgtc caccaatttt ctttggcgga
     2641 cagttttcac ttaaaaataa agaagcaact attcgtaaat tatgctaaaa tagcagtaga
     2701 acgcgtcctg agggaatcga acccccgtct caagaaccgg aatcttacgt gatatccact
     2761 acactaagga cgcaagcact gggataattg taactaaaca gataaaaaat tacaagggga
     2821 ttggacaaaa aaatgaaatt tgagcaaaat ttttcacaaa aacagcagca gacacagaaa
     2881 ctagccatga cgcaacagtt gcaacagtcc atccaagttt tacaatataa cagtgaggag
     2941 ctgttggcat tcgttgagaa tcaagcaatg gaaaatccat tggtggaagt tgttgagccg
     3001 gaatggcagc cagattacat aaaagcttct tcatcttctt atgaaggaga agagacaaat
     3061 tacctaaatc agattcctga tacaaaaggg tctttatttg attcattgat cgaacaagtc
     3121 catttgaatt accgagatac ttttttgagg aaaatcgttc tttatcttgt agaatacatc
     3181 gatttgaacg gatttttgac gattagttta gaagaagccg gaaaagagat tggagctacg
     3241 cctatccaaa tgctcgatgc gttgactctg attcagcaat tagaaccagc tggagtaggt
     3301 gcacgtagtt tgcaagaatg tctcatgctt caaacagaac gagatgatta tgcgccggaa
     3361 ttagcttata tcgtgttgga agaatgtttt gaggaactgg tggagcgaaa atggaaagaa
     3421 atcgcacaac gtttttctgt tgatctacat gctgttcagc agatattcga ctatttacaa
     3481 acgttgagtc caagccctgg ccggattttc gaccgtagca gcgaattgta cattattcct
     3541 gatgtccggg tattagtgga cgacgataaa aatgtacaag ttatctcaaa ccgaaaaaat
     3601 cagccaaata tacgctttca agagagttac ttcaaacaaa tgtcacaaca agcagataaa
     3661 gaaactgaaa attatttaaa agaacggaaa caggaattcg aatggttgaa gaaaacgatc
     3721 ctacaacgtg gggatacaat tttgcgggtt gctcaagtca ttgtttcccg gcaaaaagct
     3781 ttctttatcg acaaggaacg tccaatcaag cctcttacgt taaaggaagt tgcaactgag
     3841 atagatgtgc atgaatccac ggtcagtcgt gcagtcaacg ggaaatattt ggaaacagac
     3901 ttcggtgtat ttgaattaaa aaaatttttc actacacgaa ttcctacgaa tagtacagaa
     3961 cagacagaag acctttcagc agatacagcg aagaaaaagc tgcaggaatt agtcgatcaa
     4021 gaagacaaaa acaagccgtt gtctgaccag aaattggttg aattgctgaa aaaagatgag
     4081 atagcgatct caagacgaac agtagcaaaa tatcgtgatt tattaggaat cccgagttca
     4141 tcaaaaagaa aacgttatga taactaatca gtcaaaagac tagaaaaagt atttcacttt
     4201 ttctagtctt tttagttgaa aaacgagagc aaaactgtta gacttacagt cgtgaggttg
     4261 agatttccct ttattgatgt gaaattcgaa tagttaattt ctttttttat cgtatatggg
     4321 tcgttttaag tctacattgg acgtaaagtg tccaactaag gagatcgcta tgctggatga
     4381 aataaaaatg attgaatctg ttgcaccaga tattatcgaa gtgttgcaag aacgctataa
     4441 aattttacga aatatctatt ggatgcagcc gattggacgt cgaagtttat cggaaagtat
     4501 ggggatcaca gaacgagtat tgcgaacaga aacagatctt ttgaaaaatc tgaatttgat
     4561 cgacacctca aaaagcggta tgacgctgac aactaagggc gaagaagttt atcaaagcct
     4621 tgaaaacttc atggatcagc ttttaggtac gcatcaaacc gaacaacagt tggcagaata
     4681 ttttggtaac caacgctgta tcgttgtttc aggaaacagc gaagagcaga ccaaagtggc
     4741 tgatgcattc ggcgaagcgc tgagcgaagc cctagaccga ctacttccag aaggcgagaa
     4801 tatcatcgct gtgatgggag gaactactat ggctgtggta gccgaacaat tgtctaatct
     4861 ggaaaataag aaacggcata acctgtttgt ccctgcaaga ggcgggattg gtgaagcgat
     4921 aactgtacag gcaaattctg tcagtgcaag aatggctgct aaagcaaacg gtaaccaccg
     4981 tgcactttat gttccggaac aattaagtct ggcaacttac aattctttac tcaatgagcc
     5041 atctatccaa gaagtcttaa acttgattag tgaagcgaat tgtgttattc atagtattgg
     5101 gcgtgcatta cacatggcag cacgtcgtaa gatgactgaa aaagaattag tcatgctgaa
     5161 acaagcaaat gctgttgcgg aatcttttgg ctactttttc aatgaaaaag gtgaagtcgt
     5221 ttataaggtc cctagaatcg gcatagaatt gcaagatttg gaaaaagtcc cgatcatcat
     5281 ggcaatcgcg ggcggaaaat caaaagctaa agcaattcga gcttatatga aaaatgcacc
     5341 aaaacaaacg tggctcatca ctgatgaagc tgctgcaaat gagattttaa aaggggtaac
     5401 cctttaaaat aaatattttt ttgattttca taaggaggaa atcttaaatg acagttaaag
     5461 taggtattaa tggttttgga cgtattggac gtctagcatt ccgtcgtatc caagatgtag
     5521 atggaatcga agtagtagca atcaacgact tgacagatgc taaaatgttg gcacacttgt
     5581 taaaatacga cacaactcaa ggacgtttca acggaacagt tgaagttcat gaaggttcat
     5641 tcaacgtaaa cggaaaagaa gttaaagtat tagctaaccg caacccagaa gaattaccat
     5701 ggggcgaact aggcgtagat atcgttctag aatgtactgg tttcttcact tctaaatcag
     5761 cagctgaaaa acatttaaca gctggtgcta aacgcgttgt tatctctgct cctggcggaa
     5821 acgatgttcc aacaatcgtt tataacacaa accacgaaac attaactggt aaagaaacag
     5881 ttatctcagg tgcttcatgt actactaact gtttagctcc tatggctaaa acattgaacg
     5941 acaaatttgg tgttgttgaa ggattaatga caactatcca cgcttacaca ggtgaccaaa
     6001 tgactctaga cggacctcat cctaaaggtg acttccgccg tgcacgcgct gctgctgcaa
     6061 acatcgttcc taactcaaca ggtgctgcta aagctatcgg tttagtaatt cctgaattga
     6121 acggtaaatt agacggagct gctcaacgtg ttcctgtacc aacaggttca ttaacagaat
     6181 tagtaacagt tcttgaaaaa gaagtaactg ttgacgaaat caatgcagca atgaaagaag
     6241 cttcaaacga atcttacgga tacaacacag acgaaatcgt ttcttcagat atcgttggta
     6301 tgacttacgg ttcattattt gatgctacac aaactaaagt aatgacagtt ggcgacaaac
     6361 aattagttaa aactgttgct tggtacgaca acgaaatgtc atacactgca caattagtac
     6421 gtactttaga atacttcgct aacttataag atagacaaac gtacaagtgt aaattataga
     6481 catgtacatt taaaagcggg gaagcaatcg cgcttcttcc gctttttttg ataataagtt
     6541 tattcaggag gaaataaaat ggctaagaaa acagtgaaag atatcgattt aaaagacaaa
     6601 aaagttcttg tccgtgttga cttcaacgtt cctttgaaag acggcgtgat cactgacgac
     6661 actcgtatca aagcagcatt gccaacaatc aactacgttc tagaacaagg cggaaaagca
     6721 atccttttct ctcaccttgg acgtgtaaaa acagaagaag ataaagaagg aaaatcttta
     6781 gctccagttg caaaacgtct aggcgaatta ttaggtaaag aagtaacatt cgtacctgaa
     6841 actcgcggag aacaattaga agaagcaatc cgcaacatga atgacggcga cgttgtcgta
     6901 ttcgaaaata cacgatttga agatattgac ggtaaaaaag aaagcaaaaa tgatacagaa
     6961 ctaggtaaat actgggcttc attaggcgat gtatttgtta acgatgcttt cggtacagct
     7021 caccgtgcac atgcttctaa cgtaggtatc gcttcaactg gtatcccaac agttgctgga
     7081 ttcttgatgg aaaaagaaat caagttcatc ggtgaagcag tagaagaacc aaaacgacca
     7141 atgattgcaa tccttggtgg cgcaaaagtt tctgataaaa tcggcgtaat cgaaaacttg
     7201 attcctaaag cagataaaat cttgatcggt ggcggaatga cttacacatt ctacgaagca
     7261 aaaggaatca agatcggtaa ctctctagta gaagctgata aagtggaact agcaaaagaa
     7321 ttaatcgaaa aagcaggcga caaacttgtc ttgccaatcg acaatgtctg tgcaccagaa
     7381 ttttcaaatg atgttgaaac acaagtaatc gaaggagaca ttcctgatgg tttgatggct
     7441 ttagacatcg gcccagcatc agtaaaatta tttgctgata cattacaagg tgcaaaaaca
     7501 gttgtttgga acggacctat gggtgtattt gaaatgagta actttgctaa aggtacaatc
     7561 ggtgtttgtg aagcaatcgc taacttagaa ggcgcaacaa caatcatcgg tggcggagac
     7621 tcagctgcag cagcagaaca attaggattt gctgacaaat tcacacacat ctcaacaggt
     7681 ggtggcgcaa gcttggaatt gcttgaaggt aaagaattac caggactagc agcaatcaac
     7741 gataaataaa cgaccaagtt tgctcaattc tgacgagtaa tcgatcaaaa ggtgaaggga
     7801 gttttcatga ctccctagcc atttttcaat aaattaagga gtgtgttcct caatgcgtaa
     7861 acctattatc gcaggtaact ggaaaatgaa caaaactgca tcagaagcaa aagaatttgc
     7921 tgaagcagta aaaaacaaaa tcccttcaaa tgacaaagtt gattcagtca tcggatctcc
     7981 tgcattattc ttacaagaat taatgacatc agctgaaggt acagaactaa aaatctcagc
     8041 acaaaactgc tactgggaaa actcaggtgc gttcactggt gaaacttcac cagcagctct
     8101 tgctgatcta ggtgttcatt atgtgatcat cggacattca gaacgtcgtg aatatttcca
     8161 tgaaacagac gaagacatca ataaaaaagc aaaagctatc tttgcaaatg gtatgacacc
     8221 aatcctatgc tgcggcgaat ctttagaaac ctatgaagca ggcaaaactg ctgaatggat
     8281 cgaaggacaa attacagctg gtttagctga tctttcagct gaacaagtgt caaacatggt
     8341 tattgcttat gaaccaatct gggcaatcgg tactggtaaa tcagctgacg cacaaatcgc
     8401 tgacgaaatt tgtggagttg tacgtcaaac agttgaaaaa ctttatggaa aagaagtatc
     8461 agaagcagtt cgtatccaat acggcggttc tgtaaaacca gaaaatattg ctgaatacat
     8521 ggcaaaagaa aatgttgacg gtgctttagt cggtggcgca agcttgcaag cagattcatt
     8581 tttgtcattg ttagacgctg taaaataatt gttgaaaatt tgcgaaaaat gcaagaaatg
     8641 tttgcactaa tgagcaaaat taactaaaat cgtatatgag ggaaatccct taggaaaaaa
     8701 caaactcaaa ggagagacaa aacatgtcaa ttattactga tgtttacgct cgcgagatct
     8761 tagactcacg tggtaaccca acaatcgaag tagaagtata cacagaaagc ggagcttttg
     8821 gccgcggaat ggttccttca ggtgcttcaa ctggtgaata cgaagcagtt gaattacgtg
     8881 acggagacaa agctcgttat ggcggtaaag gtgtaactaa agctgtcgac aacgtaaaca
     8941 acatcattgc tgaagcaatc attggctacg atgttcgtga ccaaatggct atcgacaaag
     9001 ctatgatcgc tttagacgga actcctaaca aaggtaaatt aggtgcgaac gctattttag
     9061 gtgtttctat cgctgttgca cgtgcagctg ctgactacct agaagtacct ttataccact
     9121 acctaggcgg attcaacaca aaagtattgc caacaccaat gatgaacatc atcaacggcg
     9181 gatcacatgc tgataactca atcgacttcc aagaattcat gatcatgcct gttggcgctc
     9241 caacattcaa agaagctcta cgtatgggtg ctgaagtatt ccacgcatta gcttcaatct
     9301 tgaaagctcg cggattagca acttctgttg gtgacgaagg tggatttgct ccaaaccttg
     9361 gttcaaacga agaaggtttt gaagtaatca tcgaagcaat cgaaaaagct ggctatgtac
     9421 ctggtaaaga cgttgttctt gctatggatg ctgcttcttc agaattctac gacaaagaaa
     9481 aaggtgttta cgtactagct gactcaggcg aaggcgaaaa aacaactgac gaaatgatca
     9541 aattctacga agaattagtt tctaaatatc caatcatctc aatcgaagat ggattagacg
     9601 aaaacgactg ggacggattc aagaaattaa ctgacgtatt aggcgacaaa gttcaattag
     9661 ttggtgacga cttgttcgta acaaacactc aaaaattatc tgaaggtatc gaaaaaggaa
     9721 ttgctaactc aatccttatc aaagtgaacc aaatcggtac attaacagaa actttcgaag
     9781 ctatcgaaat ggctaaagaa gctggctaca cagcagttgt atctcaccgt tctggtgaaa
     9841 cagaagattc aacaatctct gatatcgctg ttgcaacaaa tgctggccaa atcaaaactg
     9901 gttctctatc acgtactgac cgtattgcta aatacaacca attactacgt atcgaagacc
     9961 aacttggtga agttgcagaa tacaaaggtt tgaaatcttt ctacaactta aaaaacaaat
    10021 aattcttttc taatgaagat acatgatcga ctattgaaag atagtcgagt atgatcactt
    10081 agttaagtaa tcgaactctc tacttgctga aatatcagca ggtagaga
//
LOCUS       DACDPS010000082        10089 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.082, whole genome shotgun sequence.
ACCESSION   DACDPS010000082 DACDPS010000000
VERSION     DACDPS010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.082"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            156..1343
                     /locus_tag="IWJ07_002236"
     CDS             156..1343
                     /locus_tag="IWJ07_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HAP9333778.1"
                     /translation="MLDEVLEIHKKNVGVLRMETTEAVQDDQDLAKMYTPGVAELSLM
                     IEKNHALARELTISGKLVAVITDGSAVLGLGNVGNQAGLPIVEGKALLYKNLAGVNAI
                     PLAIEQKSVDEIVQTIVNLQNSFAGIHLEDIAAPKCFEIEEKLQEKLSIPVYHDDQEG
                     TAIVVLAGLINAAKIQRKTLTELRVLINGMGASGVATARLLIAAGIKNLTLVDKQGRL
                     KKQDSTLNKYQRELLDHIHDTNNNASVLEDAIVGQDVFIGLSIGNVLTPEMIGKMNDN
                     PIIFALANPVPEITPDLAKESGAKLIATGSSKYPNQVNNILVFPGLFKGLLLSKANKV
                     DLSLQLEVAKQLAAKVAEPTVEKFIPNVFDEGVANVVSQAVLDYLQKLKENEDNIHAE
                     NIK"
     gene            1324..2628
                     /locus_tag="IWJ07_002237"
     CDS             1324..2628
                     /locus_tag="IWJ07_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAP9333779.1"
                     /translation="MLKTLNRIKIGFIPLSLYIILVLAYLAMTISNIVPENDMLVSLG
                     IMTVFAYLLEEIGKTIPILKDLGGKVLVVTFLPAFLVYKHWLPQSTVTVVSDFMNNNN
                     FLMFFITLLVVGSIASMNRQTLVKATSRIIIILILCDVIGSLIGTGVGILLGLSAWNA
                     YFFIVAPIMAGGVGEGAMPLSIGYAAILSGMGQNDIFGKILPCVMLGSLVAVLLSGML
                     KKIGEKYPQLNGQGNLIDGDDEHLENLKGSAKPVNIEKTLLAGVVAITIYFVAVYVEH
                     LLKSLLGFSIPSPVILLVSLMGAKMLGFIPKNIEEGGNSLYGFTVRGITPPLLFGVGV
                     AKTDWLELIEVFKNPATIVTIIVTVVAIVATAFIAAKFLKMYPVDTAIAVSCCSGQGG
                     TGALAILAAGDRMELMPFAQVAVRLGGAMTVTFAIFLMGLLS"
     gene            2654..3667
                     /gene="citC"
                     /locus_tag="IWJ07_002238"
     CDS             2654..3667
                     /gene="citC"
                     /locus_tag="IWJ07_002238"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HAP9333780.1"
                     /translation="MFDQTDIQRLFLKNNYIKDQWENLLLDSGIQKKQIEDFQHLDQT
                     LGIYHKEKLVGTVSYQNNVIKYIAVSEKYKDGGHTFNVLVSAIIQAMSQQEIFHYIVF
                     TKPRYQKSFNFLGFETIVSTPKIAVLEMGDSSIKQYLERIPKVNGEKKAAIVMNANPF
                     TEGHYYLVKQAANNNDWVYVFVLEKEQNLFSTKDRLKLVQQGLQKLKNVIVLSGGEYI
                     ISPATFPTYFLREDDEIAKEQMVVDATLFKERIATYLDISARYVGEEPLSPMTRSYND
                     ILETILPPEIKVHVLARKKTEQHQVISASQVRKAYLAGQLEKIKYMVPETTYQYLKNK
                     RER"
     gene            3671..3964
                     /gene="citD"
                     /locus_tag="IWJ07_002239"
     CDS             3671..3964
                     /gene="citD"
                     /locus_tag="IWJ07_002239"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HAP9333781.1"
                     /translation="MEINKSAIAGTLESSDVQIMISPNTEGIEIDLDSQVIEQFGKQI
                     KKVIYDTLAVYQIKNVKLKVVDKGALDCVIVARLKTAIHRSLGIQHQETPWEV"
     gene            3969..4874
                     /gene="citE"
                     /locus_tag="IWJ07_002240"
     CDS             3969..4874
                     /gene="citE"
                     /locus_tag="IWJ07_002240"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HAP9333782.1"
                     /translation="MMKERLRRTMMFVPGNNPAMIKDAGIYGADSIMFDLEDAVSMAE
                     KDSARMLVAEALKTVDYGDTELIVRINDLHSEFGRADIFAMVKAGAHVLRLPKTETSE
                     DITEVEKVIIEAENYHNVPIGRTLMMAAIEGASGVLNAPAIAKASDRLIGIAIGAEDY
                     VTNMKTKRYPDGNELFFARSMIVHAARAAGISAFDTVYSDVDDMEGFLNEVRLIHQLG
                     FDGKSCINPRQIPVVNTVYQPSIKEIKRSLDIISAIEEAKEKGSGVISLNGKMIDKPI
                     VERAQRTLMLAKASRLIDEEGNIHA"
     gene            4867..6426
                     /gene="citF"
                     /locus_tag="IWJ07_002241"
     CDS             4867..6426
                     /gene="citF"
                     /locus_tag="IWJ07_002241"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HAP9333783.1"
                     /translation="MHNKQRRSIPSSVLTKYKKEFQSVSLSPQTIQRCAPKVTTVTNQ
                     TNKLMHSIKEAIEKSGLKDGMTISFHHHLREGDFVFNQVMAVIREMGIKELTLAPSSL
                     TNTMNEMVIECIKDGTVTHITSSGMRGSLGEFISHGGMDNPVILRSHGNRPRAIESGE
                     LKIDVAFLGVPTSDDYGNANATVGKSVFGSLGYAKVDAQYADKVILLTDHLVPYPNTP
                     ISIPQTQVDYVVEVEAIGDPNKIGAGATRFTKNPKELKIAEMVKDVIVHSPYFKDGFS
                     FQTGTGGAALAVTRYLASEMEEKQIKASFALGGITKPTVDLLEKGLVEKVLDVQDFDK
                     GAAEGMARLENQQEIDASFYADPSNKGAVVNQLDVAILSALEIDTNFNVNVMTGSDGV
                     LRGAIGGHQDAATAKLTIISAPLVRGRIPTVVNDVTTLITPGKSIDVLVTEVGVAINP
                     QRKDLIAIFERIPQIPVFTIEELQQKAEKIVGIPEPLQFTDRIVAYVEYRDGSILDVV
                     RQVKEFEEERS"
     gene            6423..6980
                     /gene="citX"
                     /locus_tag="IWJ07_002242"
     CDS             6423..6980
                     /gene="citX"
                     /locus_tag="IWJ07_002242"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HAP9333784.1"
                     /translation="MMFDTGEIQEIEDILIHKEQRANLQSNLLSKYSNSSLMVFTLNI
                     PGPIKNNPMIEQVFRQGQQSIQQMFLKKKVEMTYTKEINLPSGLDWYVIVTEAPLNLK
                     KWLVTLEEQDALGRLFDLDVLFKKGEQITIVSRQALNHGERPCFICGKSAKVCARNRT
                     HTVKEMQKKIEQIVADSLKGALKHA"
     gene            6973..7836
                     /gene="citG"
                     /locus_tag="IWJ07_002243"
     CDS             6973..7836
                     /gene="citG"
                     /locus_tag="IWJ07_002243"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HAP9333785.1"
                     /translation="MHDNIQKIAQEAQLAMLYEVTCINKPGLVDPVDPGSHQDMDIFT
                     FLQSSVVLTPYFEEFIQTGLDRQHQPIEETFMAIRQIGLEAETAMFSVTNGINTHKGA
                     IFSLGIFLAICGRLTIWKVPCKKSFFQKEIQKMTKNLLNDFGKIDQTKPKAWTWGEYL
                     FVNHGLTGIRGEAKRGYPCVFDRGLPYYQAYQGSQQDKMIDTLLFLSMEVEDTNLIKR
                     SNNRHVFEEYQLLIRPYFELGGVKTIQGKKYLDYLNQQFKKKNWSIGGSADLLILTIF
                     LDKIMDKGWLC"
     gene            8070..8479
                     /gene="tnpA"
                     /locus_tag="IWJ07_002244"
                     /pseudo
     CDS             8070..8479
                     /gene="tnpA"
                     /locus_tag="IWJ07_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(9116..>10089)
                     /locus_tag="IWJ07_002245"
     CDS             complement(9116..>10089)
                     /locus_tag="IWJ07_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9333786.1"
                     /translation="SFTTRIGTLELKVPRTRDGHFSPTVFERYQRNEKALIASMLEMY
                     VSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKNYPYLMTDVL
                     YIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWITFFEYLKERGLQGT
                     ELVISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFTTIPKKNSKSFREAVKGIFKFT
                     DINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRLKSTNLIERL
                     NQEVRRREKIIRIFPNCVSANRLIGAVLIDQHDEWLSSSRKYIKFAK"
BASE COUNT         3455 a         1581 c         2024 g         3029 t
ORIGIN      
        1 ttttctcatt ataccaaaaa aattgaattt agtctttttt taaattttta aaaataagca
       61 tcattttttt aaaatttaaa atgaaagggt ttttttattt taaaaaaagt gttaaagtgg
      121 ttttgtaatt aaaaaatgta aaaaggagtg aaggtatgct tgatgaagtt ttagaaatcc
      181 ataaaaaaaa tgtaggtgtt ttacggatgg aaacaacaga agccgtgcaa gatgatcaag
      241 atttagcaaa aatgtacacc ccaggtgtag cagaactaag tttaatgatt gagaaaaatc
      301 atgctttggc aagagaatta acaattagtg gtaaattagt ggccgttatt acagacgggt
      361 cggcggtatt aggtcttgga aatgtaggaa accaagctgg attaccaatc gtcgaaggga
      421 aagcgctgct ttataaaaat ttagcaggtg tcaatgcgat tccattagcc atcgaacaaa
      481 aatcagtaga cgagattgta caaacaattg tcaatttgca aaatagtttt gcagggatcc
      541 atttagaaga tattgcagca ccgaagtgtt ttgagattga agaaaaatta caagaaaaac
      601 tttctattcc tgtgtatcat gacgatcaag aaggaacagc gatagttgtt ttagctggat
      661 tgatcaatgc tgctaagatc caaaggaaaa cattaactga acttcgagtt ttgatcaacg
      721 gcatgggagc atctggggtt gcaacagcta gactattaat cgctgctggt atcaaaaatc
      781 taacattggt ggataaacaa gggagattaa agaagcaaga tagtacatta aataaatatc
      841 aaagagagtt gttggatcat atacatgata caaataataa tgcaagcgtt ttggaagatg
      901 caatcgttgg acaagatgtt tttatcggtc tttcaatagg aaatgtttta acgcctgaaa
      961 tgattggaaa aatgaatgat aatccgatta tttttgcttt agccaatcca gtaccagaga
     1021 tcacacccga tttagcaaaa gaatctggtg caaagctaat cgctacaggt tcttcgaaat
     1081 atcctaacca agtaaacaat attttagtat ttcccggatt gttcaaggga ctattactat
     1141 cgaaagcgaa taaagtcgat ctttcattac aacttgaagt agccaaacaa ttagctgcta
     1201 aggtcgcaga gcctacggta gaaaaattca tccctaatgt ttttgatgaa ggagtcgcaa
     1261 acgtcgtttc acaggcagtt ttggattatt tacaaaaact aaaagaaaac gaggataata
     1321 tacatgctga aaacattaaa tagaattaaa attggtttta ttcctttatc tctttatatc
     1381 attttggtac tcgcgtatct tgcaatgacg ataagcaata tcgttccaga aaatgatatg
     1441 ttagtttctt tgggtattat gactgttttt gcttatttat tagaagaaat tggtaaaact
     1501 attcctattt taaaagattt aggtggtaaa gtattagttg ttacattttt gcctgcattt
     1561 ctagtctata aacattggct acctcaaagt actgtaactg tggtttctga ttttatgaat
     1621 aataacaatt tcttgatgtt ctttatcaca ctactagttg tcggcagtat tgcttcaatg
     1681 aatcggcaaa cattggttaa agcaacttca cgtattatca ttattttaat tttatgcgat
     1741 gtgattggtt ctttgatagg aacaggtgtg ggaatactcc ttgggctatc tgcttggaat
     1801 gcgtatttct ttatcgttgc gccgattatg gctggtggtg ttggggaagg agcgatgcca
     1861 ttatcgattg gttatgcagc aatattatct ggtatgggac aaaatgatat ctttgggaag
     1921 attctaccgt gtgtgatgtt gggaagttta gtcgcagttc tactgtctgg tatgctcaaa
     1981 aaaattggag agaaataccc acaattaaat ggtcagggta atttgatcga tggggatgat
     2041 gaacatttag agaacttgaa gggttcagct aaaccagtca atattgaaaa aacattgtta
     2101 gcaggcgtgg tagcaataac gatttatttt gttgctgtct atgttgagca cttattaaaa
     2161 tcactccttg gcttttctat tccttcacct gtgatcttat tagtctctct tatgggggct
     2221 aaaatgcttg gttttatccc taaaaatatc gaagaaggtg gaaattcgtt gtatggattc
     2281 actgttcgtg ggatcacacc tcccttactt tttggcgtag gggttgcaaa aacagattgg
     2341 ttagagctga tcgaagtatt taaaaatcct gcaacgatag taacaatcat tgtgacggta
     2401 gtagctattg tcgctacagc ttttatcgcc gcaaaatttt taaaaatgta cccagttgat
     2461 actgccatcg cagtttcatg ttgtagcgga caaggcggta caggtgcttt agctatctta
     2521 gctgcgggtg atcgcatgga attaatgcca tttgcacaag ttgctgttcg actagggggt
     2581 gctatgaccg taacgttcgc tatcttttta atgggtctgc tatcataaga acataaagaa
     2641 aggacgtgac catgtgtttg atcaaacaga tatccaacga ttatttttaa aaaataacta
     2701 tatcaaagac caatgggaaa atcttttgct tgattcagga atacaaaaga agcaaatcga
     2761 agattttcaa catttagatc aaacattggg tatataccat aaagaaaagt tagtaggaac
     2821 agtatcttat caaaacaacg tgattaaata tatcgccgtg tctgaaaaat ataaagatgg
     2881 tggacataca tttaatgtac tagtctctgc aataattcag gcaatgagtc aacaagagat
     2941 ttttcattat attgttttca cgaaaccgcg ctatcaaaaa agttttaact ttttaggttt
     3001 tgaaacaatc gtatctaccc caaaaatagc tgtattagaa atgggagata gttctataaa
     3061 acagtaccta gagaggattc ctaaagtgaa tggtgaaaaa aaagcagcaa ttgtcatgaa
     3121 tgcgaaccca tttacagaag ggcattatta tttagtaaaa caagctgcca ataacaacga
     3181 ttgggtatat gtatttgtac tcgaaaaaga acaaaatctt ttttcgacga aggaccgact
     3241 caaattagtt caacaaggat tacaaaaact gaaaaatgtc attgttctat caggtggcga
     3301 gtatattatt agccctgcaa cgtttcccac ctatttttta cgagaagatg atgaaatagc
     3361 aaaggagcaa atggtcgtag atgctacttt attcaaagaa agaattgcca cttatttaga
     3421 tatctctgct agatatgtgg gagaagaacc actttcccca atgactcgtt catataacga
     3481 catactggaa acgattttac cccctgaaat aaaagttcat gtccttgcaa ggaagaaaac
     3541 ggaacaacac caagttattt ctgcttctca agttcgtaaa gcgtatcttg ctgggcaatt
     3601 ggaaaaaata aaatatatgg ttccagaaac aacctatcaa tacttaaaaa ataaaaggga
     3661 gcgttaagta atggaaataa ataaatcagc gattgctgga acattagagt caagtgatgt
     3721 ccaaatcatg atttcaccga atacagaagg aatagaaatc gatttagaca gtcaagtgat
     3781 tgagcaattt ggaaaacaaa tcaaaaaagt tatctatgac acactagcag tttatcagat
     3841 taaaaacgta aaactcaagg tagtagataa aggggcatta gattgcgtta ttgttgcacg
     3901 attaaaaaca gcgatccatc gttccttagg tatccaacac caagaaacac cgtgggaggt
     3961 ttaattagat gatgaaagaa cgtttgagaa gaacaatgat gtttgttcca ggaaataatc
     4021 cagcgatgat caaagatgca ggtatttatg gtgctgactc gattatgttc gacctggaag
     4081 atgcagtgtc tatggcagaa aaagacagcg caagaatgct tgtggctgag gcattgaaaa
     4141 cagtggatta tggtgacaca gaattaattg ttcgaataaa tgacctgcac tcagaatttg
     4201 gacgtgcaga catttttgca atggttaaag ccggcgcgca tgtattacgt ttacctaaaa
     4261 cagaaacctc tgaagatata acagaagtag aaaaagtgat tatagaagca gaaaactatc
     4321 ataatgttcc gatagggcga acattgatga tggcagccat tgaaggtgct tctggtgtat
     4381 tgaacgcacc tgcgatcgcc aaagcatctg atcgattgat tggaatcgcc attggggcag
     4441 aagactatgt gacaaatatg aaaaccaaac gatatccaga tggaaacgaa ttgttttttg
     4501 ctcgcagtat gatcgttcat gctgcacgtg cagcaggcat ttccgcattt gacactgttt
     4561 actctgatgt ggatgatatg gaaggatttt taaacgaagt acgtttaatc catcaattag
     4621 gttttgatgg aaaatcttgt atcaatccac ggcaaattcc agtagtcaat actgtttatc
     4681 agccgtcaat caaagaaatc aaacgatcac tagacatcat ctcggcaatc gaagaagcaa
     4741 aagaaaaagg ttctggcgta atttctttaa atggaaaaat gatcgataaa ccaattgtag
     4801 aacgtgcaca acggacattg atgttagcaa aagcatcacg attgattgat gaggagggaa
     4861 atatccatgc ataataaaca aagacgtagc attccatcaa gtgttttaac aaagtataaa
     4921 aaagaattcc aaagtgtttc attatcgcca caaacgattc aacgttgtgc accaaaagta
     4981 acgactgtaa ctaatcagac aaataaattg atgcactcga tcaaagaagc tatagaaaaa
     5041 tcgggcttaa aagatggaat gacgatttca tttcatcatc atttgagaga aggtgatttt
     5101 gtttttaacc aagtgatggc cgtgatccgt gaaatgggca tcaaagaatt gaccttagca
     5161 ccaagttctt taacgaacac tatgaatgag atggttattg aatgcatcaa ggacggtaca
     5221 gtaactcata tcacaagcag tgggatgcgc ggcagcttag gtgaatttat ttctcatgga
     5281 ggaatggaca atcctgtgat tttacgttct catggtaatc gtccacgtgc aattgaaagt
     5341 ggtgaattga aaatcgatgt tgcatttcta ggagtaccaa catctgatga ttatggaaat
     5401 gctaatgcga ctgttggaaa atccgttttt ggttcattag ggtatgcaaa agtagatgca
     5461 caatatgcag ataaagtcat tcttctaact gatcatttag ttccttaccc taatacgccc
     5521 attagtattc cgcaaacaca agtcgactat gtcgtggaag tagaagcaat tggtgatccg
     5581 aataaaatcg gtgctggtgc tacacggttt actaaaaacc caaaagaatt aaaaattgct
     5641 gaaatggtca aagatgtcat tgttcactca ccttatttca aagatggctt ttcttttcaa
     5701 acaggaacgg gtggtgcagc tttagcagta acgcgttact tagctagtga aatggaagaa
     5761 aaacagatca aagcttcttt tgcattaggt ggcatcacta aaccaacggt tgatttattg
     5821 gaaaaaggtc ttgttgaaaa agtgttagat gtccaagact ttgacaaagg agcagctgaa
     5881 ggaatggcgc ggcttgagaa ccaacaggag atcgatgctt ccttttatgc tgatccaagt
     5941 aataaaggag cggttgttaa ccaattagat gtcgctattt tatctgcatt agaaattgac
     6001 acaaacttca atgtaaatgt tatgactggt tcagatggtg tgctaagagg tgcgattggt
     6061 ggacatcaag atgcagcaac ggctaaactt actatcatct cagcgccgtt agtacgtggc
     6121 cgaataccta cagtagtaaa tgatgttact acattgatca caccagggaa aagtatcgat
     6181 gtattagtca cagaagtagg ggttgctatc aatccccaac gtaaagatct aatagctatt
     6241 tttgaaagaa ttccacaaat tcctgttttt acaatagaag aattacagca aaaagcagaa
     6301 aaaatagtag gcatcccgga acctttgcaa ttcacagatc ggatagttgc ctatgtagaa
     6361 taccgagatg gttcaatttt agatgtcgtt cgtcaggtga aagaatttga agaagagaga
     6421 tcatgatgtt tgatactgga gaaattcaag aaatcgagga tattttgatc cataaagaac
     6481 aacgtgctaa cttgcaaagc aacctgcttt ccaagtattc aaatagttca ttgatggtct
     6541 ttacattgaa tattcctggg ccgatcaaaa ataatccaat gattgagcag gtatttcgtc
     6601 agggacagca gtcaattcag caaatgtttt taaaaaaaaa ggtggaaatg acgtacacaa
     6661 aagaaatcaa tttgccaagt ggattggact ggtacgtaat agtaacagaa gcacctttga
     6721 atctcaaaaa atggttggtc acgttagaag agcaagacgc attaggtaga ctttttgatt
     6781 tagatgtctt gttcaaaaaa ggagagcaga taacaattgt cagtcgccaa gctcttaatc
     6841 atggggaacg tccttgtttt atttgtggga aatcagcaaa ggtatgtgca cgtaatcgta
     6901 cacatactgt gaaagaaatg caaaagaaaa tcgaacaaat agtagccgat tccttaaaag
     6961 gagcattgaa acatgcatga taatattcaa aaaatagcac aagaagcaca attagcgatg
     7021 ctttatgaag ttacatgtat caataaacca ggattggttg atccagtaga ccccgggtct
     7081 catcaagata tggacatttt tacttttcta caaagtagtg tagttttgac tccctatttt
     7141 gaagaattta tccaaacagg attggaccga caacatcaac caattgaaga aacctttatg
     7201 gcgattcgac agattggttt agaggctgaa actgcgatgt tctcagtgac aaatggtata
     7261 aatacacata aaggtgctat cttttcattg gggatttttc ttgctatttg tggccggtta
     7321 actatttgga aagttccttg taaaaaaagt ttctttcaaa aagaaattca aaaaatgacg
     7381 aaaaatctac ttaacgattt tgggaagatt gatcaaacaa aaccaaaagc atggacttgg
     7441 ggagaatatc tatttgttaa ccatggattg acagggatac gaggggaagc caaaagaggt
     7501 tatccttgtg tttttgaccg aggattgcct tattatcaag catatcaagg gtcgcaacaa
     7561 gataagatga tagacacttt actttttcta tcaatggaag tagaagatac caatttgatc
     7621 aaacgaagta ataatagaca tgtttttgaa gagtatcaat tacttattcg tccttatttt
     7681 gaattagggg gtgtaaaaac catacaaggt aaaaaatatt tggattacct aaatcagcaa
     7741 tttaaaaaga aaaattggag tattggtgga agtgcagact tattgatcct tacaatcttt
     7801 ttagataaaa tcatggacaa aggatggtta tgttgatact ataatacatt attttgtatt
     7861 agtaagctaa taactaaaaa atggttcaat acaaatattt tataaaaaat ggtagtgcct
     7921 atcctttcag attgtagaca aagaaaaatt tctttgtcta caattttttt gttaatgact
     7981 tcagtcagtt tcgtgcgcaa agcgtgcgaa acaagaaaac catgcgtttc tacacgtgga
     8041 agaactagca tatcgaaagg agttttttca tgtcaaatga cgataaaagt ttagcccata
     8101 ctcgttggaa ttttaagtat catattgtat ttacaccaaa atatcgacga aaagtaatct
     8161 ataggcaatt acgaaaagat attggaaaaa tacttaggaa gttgtgcaaa ataaaaaacg
     8221 tggaaattat agaagcacat gcaacgccag atcatattca catgcttgtt caagtaccat
     8281 caaaaataag tatatccagt tttatagtta tctgaaagat cgtagtgctg tgctaatcca
     8341 cgagcagcac gtaaatcaca aatatagata cggaaataaa tcattttggt cggaggaata
     8401 ttatgtgagt acagtcgaat tgaatcaaaa gacaattgaa aaatacatcc gtgaacaaga
     8461 agctgatgat cgcatttgag atagtataag taaaagagag tagatgttgc aataacaaaa
     8521 gggtaaaaat ggactacctc cagtaaaata ccgaaaaata gtttaacttt tggagataag
     8581 tccatttaag ggtctataga atgacaattt ttttagggga ttttactatt attacttcaa
     8641 gacccgcttg gattcgcaaa gtgagtgaac tcttaaaact acctgatagt aggttgtttt
     8701 aagagttatc tgaaagtaaa aattgcattt atatttttga taaatacgta aatttatata
     8761 tacgtattta tcaaagtttt aggccaaagg aatattgtgt aaatacacta ggattgaatg
     8821 aatagaccag aagaaattgc gtccaagaac aaaagaagca tgatagtgct ctatataaat
     8881 tgggcattca agaatctgaa atttaaaggt agaattttag ttagactttt agattttaaa
     8941 cacaaaaata accatacaag caacaagaag caaagtgcta tattataaat tgtccgtccg
     9001 tttacttaca taagatcatt ggggctattt ttaaaagaga ataatagata ataaatgaaa
     9061 aaaaagtcaa gtccacaatc ctgtgtaaaa tactatacaa tgctctcact attttttact
     9121 tggcaaattt aatatatttt cttgatgaag aaagccactc atcgtgttga tcaataagga
     9181 ctgctccaat taaacgattg gctgaaacgc agtttggaaa gatacgaata attttttctc
     9241 ttctgcgcac ttcttgattc agtcgttcaa ttagattggt actctttagt cgattgtggg
     9301 aatttccttg tacggtatat tgaaaggcgt cttcgaatcc atcatccaat gatgcgcaag
     9361 cttttgaata ttttggttga tcgatataat catgaatcaa tcgattttta gcctcacgag
     9421 ctaagttaat atctgtgaac ttaaaaattc ctttaacagc ttctctgaaa gattttgaat
     9481 tttttttagg aatggtggta aagatatttc ttaggaagtg aacttggcat ctttgccaac
     9541 ttgcgttggt gaaggatttt ctaatggcag agactaatcc tttgtgcgca tcagaaataa
     9601 cgagttctgt accttgtaaa ccgcgttctt ttaggtattc aaaaaatgtt atccaggtct
     9661 cttcgctttc gccactttga atcatgaagc cgataatttc acggtcgcca tctttggtta
     9721 ttccaatcgc tatatgacag ctttttgaga gtactcgatt ttcttctcgt acttttatat
     9781 agagtacatc ggtcattaag taaggataat ttttttctga taataaacga ttctgccact
     9841 cgttaaccat tggttctagc tgttctgtta agctagaaac gaaggactta gagacggatt
     9901 taccacaaag ttcttccaca atttttgata ctttacgagt tgaaacgcct gatacataca
     9961 tttccaacat tgaagcaatg agggcttttt cgtttcgttg ataacgttca aacactgtgg
    10021 gtgaaaaatg gccatcacgt gttctgggta cttttaattc tagcgtgcct atacgtgtcg
    10081 taaagctgc
//
LOCUS       DACDPS010000083        10076 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.083, whole genome shotgun sequence.
ACCESSION   DACDPS010000083 DACDPS010000000
VERSION     DACDPS010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10076
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.083"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            64..2229
                     /gene="acm"
                     /locus_tag="IWJ07_002246"
     CDS             64..2229
                     /gene="acm"
                     /locus_tag="IWJ07_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAP9333787.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPGATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDKGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2501..2986)
                     /locus_tag="IWJ07_002247"
     CDS             complement(2501..2986)
                     /locus_tag="IWJ07_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAP9333788.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            3466..4107
                     /locus_tag="IWJ07_002248"
     CDS             3466..4107
                     /locus_tag="IWJ07_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9333789.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            4126..4761
                     /locus_tag="IWJ07_002249"
     CDS             4126..4761
                     /locus_tag="IWJ07_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9333790.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4779..5594
                     /locus_tag="IWJ07_002250"
     CDS             4779..5594
                     /locus_tag="IWJ07_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9333791.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            5913..7349
                     /locus_tag="IWJ07_002251"
     CDS             5913..7349
                     /locus_tag="IWJ07_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9333792.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7538..9985
                     /locus_tag="IWJ07_002252"
     CDS             7538..9985
                     /locus_tag="IWJ07_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP9333793.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
BASE COUNT         3508 a         1664 c         2177 g         2727 t
ORIGIN      
        1 aaggattttt gagagatgat atagtaggtt ttatttttag ttaaaatagg ggagatagaa
       61 aaaatgaaaa agtgtagaaa tattatttta tcaatgttat ttattattac taatataaca
      121 tcactgattc ctgttcatgt ctatgcggat gcaggcagag atatcagcag taatgtcact
      181 tcgttgaccg tggacccaac gaatattaca gatggaggaa acataaaagt aaaattctcg
      241 tttgacgaaa aaaaacaaaa tatccaacca ggtgattatc tttggatcaa ttggccaagt
      301 gaaggaaata ttcgcggtga aggattccaa aaagaaatcc cattaatgat tgaaaataaa
      361 aatgttggaa ctttaacggt cagaaaagat tctgcgcagg ttgtgtttaa tgaaaatatt
      421 aaaaacctag actcagtaga aggttggggg caatttgaga tacaggccag aaacgtaacc
      481 gataccggtg aagaaaatac gggaccattc accgtgacga gcggtgataa aacagctact
      541 gtaaatgtga caaagccagc ttctggttct tcttctagcg ttttttatta taaaacagga
      601 gacatgctac ctgaagatac taagcacatt cgatggtttt tgaatattaa caataacggc
      661 acttatgttg aacaaccagt taaaatcagt gatgaaatcc aaagtggaca aagattagat
      721 ccaagtacgt ttgaaattaa tcaaatacac ttaggtgaac agaaggttta tagaggagaa
      781 gagggaatcc aacaattttt acaagatttc cccggcgcta catttaattt ttcagtaact
      841 gataattaca ttgaaataac tataccaaaa aactttgtca atcttaggaa aataatggtg
      901 agttataaga cgatcataga aaacccagaa caaataaatt ttgagaatca ttcggaagct
      961 tggtttaaag aatttaataa acctgctgtt gatggagaaa gtttcaatca tacagttaaa
     1021 aatatttcag cttctggtgg agtcaacggt acagttagag gcgaattgaa gattttcaaa
     1081 tatatcaatg acacagagat tggtatccca aatgtgactt tcgaattaag acgagcagat
     1141 gaacagccta ttcaaggaca gtctagtatc ctgttgactt cgaatgaaca gggagaagct
     1201 agtatcaaag gcctgcaagt aggagactat gttgtaaaag aaaaagaagc acccaattgg
     1261 attgattttg atccactaag ttcaaatgaa ctcaaattct ccataaacga aaatgataca
     1321 gagggagtca gtttacctat ttataacaag aaaaaggtta cgaacatcac agcaactaaa
     1381 atttggaacg gtggaaccac tccaagaccg agtatttatt ttaaattatt cagagcaagt
     1441 acaaacaatt gggaaccagt tcctgatgca gaaacaaaac gattagacaa aggaataact
     1501 tcagtgactt gggaagatat ccaacagtat gatgatagtg gaaacgaata tacgtttaaa
     1561 gtgcaagaag tagatcaaaa cggaaatgat tatgtccctt caggttatca aaaaattgaa
     1621 aacgggctag tcgttacaaa tgagaataaa gaagtcattt cagtctcagg acaaaaaaca
     1681 tgggaagaca atgagaacca agacggcaaa cgaccgacga cgatcacagt aaatctatta
     1741 gccgacggtc aaccgatcca acacaaagaa gtaagtgaaa aagatgattg gcgctatcgt
     1801 ttcacaaatc tgccaaaata cagagacggg caagaaatca tctatactgt aacggaagac
     1861 aacgtgcccg aatacagtac gacgattgac gggtataaca tcaaaaacag ttatacaccg
     1921 gaaaaaacaa atatatctat accaggtagc catataacac cgtggaaaaa ttttccagag
     1981 aagacagaga gagagaaaat tagaaatcta ttctctcaaa aattgcaaat atttgctcat
     2041 acaggagaat ccagcgaaca aagaagcaat tatattagta agagagctgt acctaaacag
     2101 ataacaaata aatcaaaaag cattcttcct aaaacaagta gtaaaaaatc tagttttgct
     2161 gtaatcattg gtttattgat agtaactatc tgtgctggga tattcttaca aaaatataaa
     2221 aaagtttagt tagaatataa tattttttgt taaatatgac tgaaatcaaa agaataaaat
     2281 tccgatttat aatcgaaaaa agaagcattt ttatatatgc ttcttttttc gtagaatcaa
     2341 taaatatgga tttggctaac tatggaagaa tactcgtatt agttttcagc gttcatacta
     2401 gttgttacat tgtttgattg gccaaaagaa tttctgatat attttgtgct aaataaagaa
     2461 aaagacgctt gaattgggaa ataagaaaac tactctatca ttacaaattt tcataaactg
     2521 gcagatgttc taagtaaaca agtatctttt caaagtcgga aaaatccata atcaacgtag
     2581 tggtattgat atttggatga aagcctacgg tatcttgacg atcaatagca gcatcgatca
     2641 cgacttggat ttttttctct ttgtcgtgca tcaatgctaa tggagtcact gtacctgctg
     2701 gaacgcctag gaggtcagga agctgttctt cagaaacaaa agataagcgc ttttctttta
     2761 attgttcagc tagttgtttt aaatcggcgt gcttttcatc aggcaagatg acaagataga
     2821 tttgttttcc ttttcttgat ttcaataata gattttttac ttgcggacca ggtagggaaa
     2881 aagggacatt tttgactgac gtaatcgcag gatgatcgac tttttgaaaa gagataccca
     2941 gtttttttag tagttcataa gcttcttgtt cggttgcacg actcatcgcg catcaccctc
     3001 ctttttttat tagtgtatca taaaaaagag ttgacaaatg aaaaataatc atgtaaatta
     3061 cacgtaaagt taaaacaaat gacatcgaaa agaagagtag ccgttaggtg tttttagaga
     3121 gtctctggtt ggtggaaaga ggcagcagtt aaccgtgaaa cacatcttgg agtcgatttt
     3181 ctgaagcgta tagtaggaaa atccggggag cccgttacag ctgcggtcgt tgctagatga
     3241 ccagataagg ctggcttttc cgtgaggagt cggtaaaaaa aggtggaacc acgtgattaa
     3301 cgtcctttga tcttcggatc aaaggacttt ttgttttcat ctgaacggca acgactgtgt
     3361 gaggtttttt ctgcaaggaa aaaataaaca gaggtggaac cacgtataat acgtcctctt
     3421 ggcatttgcc aagaggattt ttttatttta aaggaggaaa agaagatgga gtatttgatt
     3481 gaagttatgc cgcaattatt agtaggagca ctcacaacat tgaaattatt tttatttacc
     3541 ttgattggct cattaccatt agggattgtt ttagcattag gattgtcttt gcattttaag
     3601 ccgctacgct acttgcttca aacatatgta tgggttatgc ggggaacacc gctgttgttg
     3661 caactgattt ttatttttta tgggttgccg acgattggga tcgtttttga tcgtttcgaa
     3721 gcagcactca ttgcctttat cttaaactat gcagcttatt ttgcagagat ctttcgtgga
     3781 gggatacaat cgattcctca aggtcaatat gaagcagcgc aagttttgca attaagtccc
     3841 tttcaaacgg tgacgaaaat cgtcatccca caagtgacaa agattgtttt accttctgta
     3901 gggaatgaag ttatcaactt ggtcaaagat tcttccttga tctatatatt aggtttaggt
     3961 gacgtaatgc gtgctgggaa aatcgcgatg gaacgcgacg cgactttact gcctttgcta
     4021 ggcgtggcaa tcatctatct tacattgact ggtattctaa caatcgtaat gaaacaagtt
     4081 gaaggaaaat taaattatta ccgctaactg ataggaggaa gaaatatgat tgaattgaag
     4141 aaagtgaaca aagcatttgg tgaaaaaaaa ataatcaaag atttgaatct aatcattcca
     4201 gatgggaaaa cattagctat cgtaggtcct tccggtggcg gtaaaaccac gcttttacgt
     4261 atcttggctg gcttagaaag ttctgacagc ggagaatttt atttagacgg tgtaccgttc
     4321 aacccattcg agcttaaaat gaaagaacaa gttgtaggag tcgtattcca agacttccag
     4381 ctatttccac atctgaatat ttttgaaaac attaccttag caccaaaaat ggtattgaaa
     4441 cagacaaaag aagaatacac caaaaaagca gaagaattag cggcttcatt agggattagt
     4501 gatttattga atatctatcc ttatcagctt tctggcggac aaaaacagcg ggtagcgatt
     4561 gccagagcat tggctatgaa tccacgggta ttggcttatg atgaaccaac aagtgcttta
     4621 gatcctgaat tacgccagca agtcgagcgt ttgatcctat cattgaaaga agatggggta
     4681 acccaaatta ttgtcaccca tgatttagta tttgcagaaa atgtcggtga tcaattacta
     4741 aaagtttccc caattcatta agaaaggaga agatagagat gaaaaaaacc agttttctat
     4801 tcgctttgat cgcaggtctt cttctttttg caggctgttc gaacaaaaaa acttcagcag
     4861 ataattgggc gactatcaac gaagagaaaa aaatcgttat aggtttagac gatacatttg
     4921 ttcctatggg atttagagat aaagacggga agctcacagg atttgatatt gatttagcca
     4981 aagcagtctt tgaacaatat gggattacgg tagactttca gcccattgac tggtcaatga
     5041 aggaatttga actgaacaat ggaacgatcg atttgatatg gaacggctat tccaaaacgc
     5101 cggcaagaga aaagaaagtg caatttacaa aaccttatat ggaaaacgat caagtgctga
     5161 tcacacctaa agaatcagga atcacaaact ttggacaaat gaataataag agattaggtg
     5221 cgcaaaatgg gtcatctggt tatgatgtat ttacgaaaca accagaagta ttaaaagaga
     5281 tagtcaaaaa ccaagatgct gttttgtatg atagttttaa tgaagcattg atcgatctga
     5341 aaagcagacg ggtcgatggt ctgctgatgg ataaagtata cgcagactat tatttgaaac
     5401 aaagaaacga actagaagac ttcaacataa caaaaggttc ttatgagagt gaggattttg
     5461 cagttggtgc acgcaagtca gatagtacat tagtgaaaaa aatcaatgaa gccctagatc
     5521 aactagagaa aacaggtgaa ttccaaaaaa tatccgataa atggtttggc gaagatgtta
     5581 cgccgagaaa ttaacaaata ggggactgtg acaaaagacg tgtcacagtc cctttttccg
     5641 aataaacagt gttctcctgc caactccttg tgactcggag ctgaatggca ggctcacaac
     5701 ctcttggtaa acggtgttca aaagccagtt tttcggtatt aagccaaatt tatgcaaaaa
     5761 tgtagaaagc cattttcaca aaatttttct tactactcga aactaagcga ctttttcaca
     5821 acctccttta cataatttta ttttgttgtg gaaggggttt cgtgttattc taaaagagga
     5881 acgattgaag gttataggaa aggaagagat gaatggaaca caaagaactt acctcatttc
     5941 caaaagattt tctgtggggc tctgcttctg cagcgtatca agtagaagga gcttggcaag
     6001 aagatgggaa aggagaatca gtctgggatc gatttgtacg gatacctgga aagacattta
     6061 aaggaacgaa cggtgatttg gcagtagacc actatcatcg ttataaagaa gatattgctt
     6121 taatgaaaga acaaggtctg aaagcttacc gcttctctgt agcttggaca cggatttttc
     6181 ctaatggacg aggagaagtc aatcaagctg ggctcgcttt ttatgagaga ttgatcgacg
     6241 aattgattga aaacgatatt gaacctatgt tgactttata tcattgggat cttccacaag
     6301 cattgcaaga tgaatataat ggttgggaat cacgtcagat cattgaagat ttcactcatt
     6361 atgcagaaac attatttgag gcattccgcg ggaaagtcca ttactggatt agtttgaatg
     6421 aacaaaatat cttcacttca ttaggttatt tgttagcagc acatccgcca ggtgtgacag
     6481 acccgaaacg aatgtatgaa gttaaccata ttgctaattt agcaaatgca tctgtcatca
     6541 ataaatttca cgagttagaa atgcctggaa aaatcggacc aagctttgcg tatacgccaa
     6601 attatccaat tgacagtaat cctgaaaatg ttctggctgc tgaaaatgca gaagacctga
     6661 tggccaatta ttggatggat gtctatatgt ggggaaaata tccgattgct gccatgaggt
     6721 tcttagaaga aaaaggatgg gcaccaacga ttgaagctgg cgatgcagaa ttgctagaat
     6781 ctgctaaacc agattttctt gggatcaatt actatcaaac ggcgacgaat gcattcaatc
     6841 cattaaacgg agttggtgca ggaaaaatga atacgacagg aaaaaaaggt tcatcagaag
     6901 aaactggagt tcctggaatg tataaaaaag ttgagaatcc atttgtcgaa cgaacaaatt
     6961 gggactggga gattgaccca gaaggattaa ggatcggact gcgtcgtatc acaagccgtt
     7021 accgcatgcc ggtactgatt acggaaaatg gcttaggaga gtatgacaag ttgacagaag
     7081 ataaacaaat ccatgacgac tatcgaatca attacttaca aagccacgtc aaagccatca
     7141 aagaagcaat ttcggatgga gcagaagtcc taggctattg cacctggtct tatacagatt
     7201 tgcttagctg gctgaacggt tatcaaaagc gatatggatt tgtatatgtt gatcaagacg
     7261 aaacacaaga aggatctttg gaacgatata agaaagacag ttattactgg taccaaaaac
     7321 tgattgaaga aaatggagag aacctttagt tcacatactt aaaacaagaa ggtacggctg
     7381 gatatggccg tgccttttct tgataaaatc atttagaaat taagaaaaac tgtaaaataa
     7441 gctgaaaaat gaaagaaagc gtcagttctt tcaaacaaaa ctttagttcc cttacttttt
     7501 tttgtataat gaaacttgat gaaatgaggg ataataaatg gcacaaaaga aacggcctac
     7561 taaaaaaagg aaaaagacga aaaaaacgaa aaagcaaaaa cagcagcagg aacaaatgct
     7621 gacgattggt gttgggtttg ccttgatttt atttgctgtc ttcggctttt taaaattagg
     7681 ttttttagga atattgattg caaacggctt tcggatcatt gcaggaaata cttaccaaat
     7741 cctctgtctt ttattagccg tattaggatt ttggattgtc attaagaata cagagttttc
     7801 aattggtaaa aaccgacgct ggtttggcgg catactcttt tatttcggta tcttattatt
     7861 gttacatgcc catttatttg gcaaactcca tactggggaa ccaaatatca tggggacgac
     7921 atgggatttg ttagccagtg atataaaaca aagtcaagta gacaataacg ttggtggtgg
     7981 gatgattgga gcaatccttt atcattttac ttacttcttg attgcacagc ctggaagtta
     8041 tttagcagca gtgctattac tggcaggagg agcattcctg ttcagtaatc tggaaggcta
     8101 tcagctgtta aatggtatcc aatcaattgg agaacgagta caggaattat tggaaggtga
     8161 tcctgccaag caagctcgaa agcaagcggc aaaggaagag agaatgaaac agcgcgcaga
     8221 agcgaaagaa gcaaggcgct tagctgcaaa agaagctgca gaaaaagaag ctgcagaata
     8281 tgagaagaaa aaagcggtga gccagcaaag ggaaaatact gctgatgaat ggcaggaacc
     8341 agaaaataca gagccggagc agctaagttt tgttccaatc gacagttttc aagaaaatat
     8401 ccatcctgcg aatctagaga aaccagtacc agatacacca aaacagacca atacagcaga
     8461 aggtttttca gatgagttgc cagaggatga cggtacctca ttagaatttg agattgaagc
     8521 agaacaggaa aatcaagact atgaattacc gacagttgat ttgcttgatt ctataccaac
     8581 tgtggatcaa agtgatgagt ataaaaaaat tgaaaaaaat atcggcgtat tagaacaaac
     8641 tttccaaagt ttcggtgtcg atgccaaggt cgtcaaagcc agcttaggac catccgtcac
     8701 aaaattcgaa gtccagccgg ctgtaggggt aaaagtaagt aaaattgtga acttaacaga
     8761 tgatatcgcg cttgctttag cggcgaaaga tgtccggatg gaagcaccga ttccaggtaa
     8821 atcactgatt ggtattgaag taccaaatgg aaagatcagt atggtttcct ttagagaaat
     8881 catcgaagca caaccaaacc acccagataa attactagaa gtacctttag gacgtgatgt
     8941 ttctggacgg gtacaaacgg ctgatctttc gaagatgcct cacttactcg tggctggttc
     9001 gactggtagt gggaagtcag tagcaattaa tgggattatc acaagtatat taatgcgtgc
     9061 taagccccat gaagtaaaac tgatgatgat cgatccgaaa atggttgaat tgaatatgta
     9121 caacggcata cctcatctgt tgacgccggt cgtgacgaac cctagaaaag cagcgcaagc
     9181 cttacaaaaa gtagtacaag aaatggaaga acgatatgaa aaattcgcgg caaccggagt
     9241 aagaaatatt tctggttaca atgaatttgt tcaacagaaa aacttggaaa acggaacgaa
     9301 acatccaacc ttaccattca tcgtagtgat cgtagatgaa ttagctgatt tgatgatggt
     9361 agccagcaat gaagttgaag atgcgattat tcgtttagcg caaatggctc gggcagcggg
     9421 gattcatatg atcctagcta ctcaacgtcc aagtgtggat gttatcacag gaatcatcaa
     9481 agcaaatgta ccttccagaa tggcatttgc ggtatcaagc ggtacagatt ctcggacgat
     9541 cattgattct aatggggcag aaaagctctt agggcgagga gatatgctat tcttgccaat
     9601 gggtgagaat aaaccgatcc gtgtgcaagg agcattcatt tctgaccacg aagtagaacg
     9661 ggtcgttcaa tttgttacag atcagcaaga agctcattat gaagagaaaa tgatgccgac
     9721 agatgaagtt gaaacgacag gtgctccaga acagccacaa gatgagctgt ttgaagaagc
     9781 aaaagcactt gtcgttgaaa tgcaaacggc aagtatttcc cttctccaaa gaagatttcg
     9841 gatcggttac aaccgagcag cacgtttagt agatgaactg gaagctcatg gagttgttgg
     9901 cccgtcagaa ggaagcaagc ctcggaaagt attcattgag caggaagaaa catcagaaac
     9961 gttgccggac gataatgaca tgtaaaactt gtgtgatgaa gggttgaaaa acgaataacc
    10021 aataaaaaga caaggcatga tgataccaaa agaaaaaatg gtttttttcc atcttt
//
LOCUS       DACDPS010000084         9823 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.084, whole genome shotgun sequence.
ACCESSION   DACDPS010000084 DACDPS010000000
VERSION     DACDPS010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.084"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(46..2166)
                     /locus_tag="IWJ07_002253"
     CDS             complement(46..2166)
                     /locus_tag="IWJ07_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP9333794.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2396..3574
                     /locus_tag="IWJ07_002254"
     CDS             2396..3574
                     /locus_tag="IWJ07_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAP9333795.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3590..4348
                     /gene="aroD"
                     /locus_tag="IWJ07_002255"
     CDS             3590..4348
                     /gene="aroD"
                     /locus_tag="IWJ07_002255"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAP9333796.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4371..4856
                     /gene="ybaK"
                     /locus_tag="IWJ07_002256"
     CDS             4371..4856
                     /gene="ybaK"
                     /locus_tag="IWJ07_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAP9333797.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4927..6180)
                     /locus_tag="IWJ07_002257"
     CDS             complement(4927..6180)
                     /locus_tag="IWJ07_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9333798.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVYLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6297..7646
                     /gene="gdhA"
                     /locus_tag="IWJ07_002258"
     CDS             6297..7646
                     /gene="gdhA"
                     /locus_tag="IWJ07_002258"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAP9333799.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7758..9104
                     /locus_tag="IWJ07_002259"
     CDS             7758..9104
                     /locus_tag="IWJ07_002259"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAP9333800.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9412..9798)
                     /locus_tag="IWJ07_002260"
     CDS             complement(9412..9798)
                     /locus_tag="IWJ07_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9333801.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3134 a         1785 c         2012 g         2892 t
ORIGIN      
        1 agcagcgaca ataattttgt cgctgctcct tttaaattta ttttatcatt cggaagaact
       61 tgtggtcgtt ccgtgaagtt cttggtacgt ttgcgtttta taaagatcaa cagttgattg
      121 tccgttgtgt tcactaaaga cactagttga cttgtcacca agagattttt cagcttcttt
      181 taatttctct aagccattct tgtagtcata atcactggta tcaattgggt ctaatccact
      241 attcgtatag aaccgaagaa gatcaccatt attgatttgg tcagaaacag ctaactgttt
      301 atcaactttt tctttcagtg aatcaatttc attctgtact tcctcagtag gttcagtaat
      361 agcttcacca gtttcgttac tatagatcgt accaccgtag taagtatatt ttggtgtaac
      421 aaaatcacca tcacggaaag aaacaagctg ttcatgatct ttcgagaata gatcttgtcc
      481 taactgaata taattttttg tatctacacc tagtaagtga agtaatgtag gcaaggcatc
      541 aacttgtccg ccgtaagtgt gattgattcc gccctgtgtt tgtccaggga tatggatcat
      601 ataagggaca cgctgcattt gtgcattgtc gtagtttgtc caagtagaac ttgtttttcc
      661 aactaattca gctagatttt tatttcttga attcgagaca ccgtaatggt caccgtaaag
      721 gacgatgact gaattatcat acaagccgct ttctttcaaa taattgaaga attcttgtac
      781 ggcggtatct aaataattgg ctgtcgcaaa gtaaccgttg atcgtttcat catttgtatt
      841 ggctaacggg aatcctgctt cgtcattggt gaattttgaa taaggataat ggttagatac
      901 agcaatgaat tttgaataaa atggttgttg cagacgttcc aagtattgta cggattgttg
      961 gaaaaatggt ttatcatgca acccgtattg gaacgaattg tcatcattaa catcataata
     1021 agaagaatca aagaaataat catatcctaa attcttgtat gtctcgtttc gattccagaa
     1081 tgtaccagcg ttcccatgga ataccgcact aatgtaacct tgtgtttgac ttaaaatatc
     1141 aggtgcagct tggaaagtat tttttccgcc taattgtgtg aacaaggagc cttgattcaa
     1201 gccaaacaat gaattttcca tcagcgtttc cgcatcgctt gtttttcctg cttttacttg
     1261 atggaaaaag ttgtcaaaac tgaacgtgct gttgctgtgg taaagactat tgatgaaagg
     1321 tgtaacttca tgctggagac cattctcatc tgtcaattta taatcgatca ggaattgctg
     1381 gaagctttct aaatggatat aaatcacatt ttttcctcta gcgagcccga acaaatcgtc
     1441 atttggtgct gcataatgtt cttttacata attttctact tctttcatat cattgggact
     1501 tgcttctgcc cgcacttggt ttgtctggta agtttgaaca ccgtcataaa ccatgaaagc
     1561 attcaagcca agatatttga ccaaataatc acgagagaat gttcgagtca gcaattctgg
     1621 acgatcagct tccgccataa aaagatttcc tgagaaaatc ataactgcta atgtagagat
     1681 cgcaaaagcc attcttgctc ggatcgggcg agggtccaac ttgatttttt ttgcaatcag
     1741 cagaacagtc aagataataa aatcgatcca atagatcaca tcatatgggc gaaacagtcg
     1801 aatggcactt tctcccagac cactcgctac tttcccagca cctaaaatcg tgttgatcgt
     1861 aatgaagtcc gtgaattctc gataataagt gacattcgaa aaaacaagca atgtcaaaag
     1921 aaaataaatg accatcatcg tgatataagc aactctaggg cgtctgatat aaagagcaat
     1981 tccaagtaat aacaatgtag ttgcaatagg gttgataaac aagataaagt attgaatgcc
     2041 actttcgatt ccgagatgga aatcaactaa ataggcaaaa atcgttttca accaaagtaa
     2101 aacgactagt aaagaaaaaa atccgagcct cttattcaaa aaggcaggtg tttttataca
     2161 tttcaagata atcttccttt ctgcacttct taatcacaga agtgtcctga aaccatgtta
     2221 cacagctcac ttattctcat ttattttact agtatgacaa tatagtgtca attgaaacca
     2281 gaagtttatg ctgtttcatt aaggttttct ttaagcttca tgaaaaaatg agcagtaaga
     2341 caagtcctat gttttttgtt atacttggga agagaaagct aaagcaggtg aaggaatgaa
     2401 agtaaaagta aaaaaaagag caagcaatcg attgaaaaaa ggatatccac tgatccaaaa
     2461 agaagacctt gctcatcccc aaaaagaaac cacgcaatgg gtggaattag ttgattcgca
     2521 aaaccaattt atcgcaaaag gatatctagg aaagcagaat aagggaatcg gttgggtatt
     2581 gagccagcag aacatcccat ttgatcaatc tttttttgaa gaattttttg tgaagtcaaa
     2641 agaaaaaaga actttctttt tttcagatga ttctactacc gcttttcgta tattcaatgg
     2701 agaaggagac gggatcggcg gtctgattgt tgattattat gcaggttatg ctgttttttc
     2761 ttggtataac gaaacgttat atgcacatcg tgaattattg attcaagctt ttcgtgaaat
     2821 ctacccggaa atcataggag gctatgagaa aattcgattt gaatcagtaa atctgcctga
     2881 gagccagttc atttacggaa aagaggcacc ggaacccttg atcgtaaaag aaaacggagt
     2941 aaaatttgcc gtttatttaa atgaaggatt aatgactggg atctttttag accaaaaaga
     3001 agtacgggga tcattagcag aaggtttcgc ttctgggaaa acagttttga acatgtttag
     3061 ttataccggc gccttttctg ttgctgccgc aatgggaggg gcaatcgaaa caacaagtgt
     3121 cgatctagca aaacgcagtt taaaaaaaac acaggaacag tttgaagtga atggtttgga
     3181 tgtcgatcag caaaaaatca ttgttatgga cgtattcgag tatttcaaat acgccaaaag
     3241 aaaacaattt ttatatgaca tgattgtatt ggatccacca agttttgcca gaaataagaa
     3301 aaaagtcttt cgcgtagcaa aagattatgg ggaactggtc aaggatagtc tggatatttt
     3361 agcagaagac gggatactta ttgcgtctac aaatgctgcg aatgtctcga tagaacaatt
     3421 ccaaacaatg attgaagagg aattccatgc caaaaatgtt tcttttgaac aacttgcttt
     3481 atatcgtctg ccaaaagatt tccagacgac ggataccttt ttagaaggca attatctgaa
     3541 agtgtttgtt tatcaaatta aaaataacca ttagaaggag cgatgcagaa tggttcgggt
     3601 aaagggattg aatataggaa gcggtagacc aaagatttgt gcgcctctta tcggggccgt
     3661 ccgagaggaa gtattacgtg aagcagttct tgcacgtgaa gcaggggccg atttagtcga
     3721 atggcgagtc gatcattatc gtgaagtttt ttcttttgat gaagtagcag aaacattggt
     3781 tcttatccat gagatgcttg atggactgcc gttattattt acgtttcgta ccttagctga
     3841 aggcggagaa caagacatat cgatccgtca gtatcgcgac ctttatgaac atgtgataca
     3901 cacaggatta gtagatatgg tcgatatcga gttgttcaaa gtagaaagtc taggaaaaag
     3961 actgatagag gaaatcaagt ctttaaacat tcctttaatt atcagtagtc atgattttaa
     4021 agaaacacca gcagaccctg tcttgcttta ccgattgaat gttatggaac attttggagc
     4081 cgatatcgga aaactggcag taactcccaa taatgaacga gatgttttgc ggttgatgga
     4141 gttgactaga cgggcaaatg cttttgtctc catgcctatt attacgatgt ccatgggaaa
     4201 tcttggtaaa atttctcgtc tggttggcgg aacgacgggt tccgttatga catttggttc
     4261 cttaagcagc gattctactt ctgctccagg acaattaccg gtcaaagcat tgaagcaaat
     4321 tattcaatta ttaaatgaac cgttgtagaa caagtttgag gtgatatttt ttggcaaaga
     4381 aaaaaatagt aaaaacgaat gcaatccgta tggtcgaaca aaaaaagatt ccctatacag
     4441 agcacgaata tgagtgggac gaaagccacc taagtgcgtc atcggtcgct gaacagcttc
     4501 cagaaagcca atctcgtata tttaaaacac tggttgctgt gggcaatgtg acggggcctc
     4561 ttgttgctgt gataccggga gaagccgaat tgaatctaaa aaaattagca aaagtttcag
     4621 ggaataaaaa agtagaaatg ctccatttaa aagatttgga agcaacgacc ggatacatac
     4681 gtggtggctg ctcgcctata ggtatgaaaa aactatttcc tacttattta gatcaaattg
     4741 cagaaagcta tgaacaaatc attgtctcgg ctggcagaag agggctacag atggagttag
     4801 cacctcaaga tatctgtgca ttaacgagcg gacaatttgc agatataaaa caataaatca
     4861 tacgtataaa agaaagaagg aggccgggat gttgtttgtc cagtctcctt ctttcttttt
     4921 tatacattat gctctttgcc cggcattcat tcctgctaca tgccctgtag cgaaagccgc
     4981 tgtgatatta aagccgccag tataaccatt gacatccagt aattctcccg caaaatacag
     5041 accatcaatg attttactct ccaatgtttt aggataaatt tcctttagag aaacccctcc
     5101 gccagttaca aatgatttct caataggaaa agttttgttg attgatagct taaactcttt
     5161 gcatagttga acaaaatctt gaatttgttt ttgagaggct tgttgtcctg tcagatgatc
     5221 catttcaagc cgctcaagaa agaaaactaa caatcgctca ggcaatatac catgccaagc
     5281 attgaccaag ttctttttgg ttgctttact tttttctgtc aatatatgaa taagctcttg
     5341 tttggtttgt gtagggaaac aatccagaga aagtgtcact ggttctcccg ttttctctaa
     5401 ttctttattg acaaaacttg aacaacgcag tgcagccgga ccagaaatac cgaaatgggt
     5461 gaaaagtaaa tccatcgtat gttcagtcaa tacacgacct tttttattta aaacgcgtaa
     5521 agtgatgtct tgtaaagaaa gcccttgaag tgttttctcc tgaatatacg gttcatccga
     5581 tatcaagggg gactctgtcg cataaagagg cgtcaccgtg tgacccactc gcttcacgat
     5641 cttgtaccca tctccagtag aaccagtaga tggataagtc cgtccaccag ttgttaagat
     5701 aatggcagga gcatggaact cttcgaattc cgtgcgtata ccgtaaattt tatgttcttt
     5761 gtggatcaat ttctctactc gagtagagaa tagaagcgtc acatctagtt ctttcagacg
     5821 attgagcaat gcttcgataa tcgtttttga tttatttgtt acaggaaaca tccgtccatg
     5881 atcctcttct tttaaatata cgccttgtga ttcaaagaag ttcatgatat cgaaattatt
     5941 ccattgcgca aacgtactat ataaaaactt tccatttcca ggaatatgtg caatcaaatc
     6001 atcaacagga cgattgttcg taacattgca ccgaccacca cctgtcatca gtagtttttt
     6061 tcctgctttt ttgttttttt cgatcaagag tacgcgtgcg ccttgttcag ctgcactgat
     6121 cgctgccatc atcccgcttg ttccagcgcc gactacgatc acatcataca gtttttccat
     6181 tccctcacct caaggaaagt gtagcatatc tgaagaaaga aaacagaaaa aaatgttttg
     6241 agccaaaaga gaaaatgagc taaaatgagg gaagataagc aaaggagcgt ggaacgatga
     6301 caaacgcaac agaatatgtt caagcaattc aagaaaagct acatcaaaag gatcaagggc
     6361 aagaagaatt tttacaagca atcgatgaat tcatgcccac ggttatgtct tttttggaca
     6421 ctcatcccga atatatcgaa aagaatatct tagaactttt aacggaacca gaaagagtta
     6481 ttcaatttcg agtgccttgg caagatgatg aaggaaactg gcgagtaaat agagggtacc
     6541 gtatccaata caactcagct atcggccctt ataaaggtgg attgcgcttc catcctagtg
     6601 tcaatctaag tatcttgaaa tttttggctt ttgaacagat tttcaagaat agtttaaccg
     6661 gtctgccaat cggtggagga aaaggcggaa gtgacttcga tcctaaagga aaatctgata
     6721 atgagatcat gcgtttttgt caaagtttca tgctggaatt gtcaaaacat atcggcccat
     6781 cgattgatgt tcctgcggga gatatcggtg taggagctag agaaatcggc tatttgttcg
     6841 gcgaatataa acgattgaaa aaatatgata ctggtgtgtt gacaggtaaa cctttggatt
     6901 tttggggaag taagatacgt acggaagcta ctggttatgg attagtgtat tatgtcaaac
     6961 atctattgaa cgaagaaaaa gattcattta atcaaaaaac agtttttgtt tccggaagcg
     7021 ggaatgtagc catttatgca atcgaaaaag tgcaagagct aggcgggaaa gttgttacat
     7081 gttcagattc tagcgggtac atctatgatc cagaaggaat agatgtgaaa ctattgaaag
     7141 aagtcaaaga aatcaataga caacgtttga cggaatacgc aaaccaacgt ccttctgcta
     7201 tttatcatgc tggagaatct gtttggacat tgaaagaaaa agcggatatc gctttgccat
     7261 gtgctactca aaatgaaata gacgaaagct tggccgaaat cttagtagaa aatgggatca
     7321 aaatcgtagc agaaggcgct aatatgccat gtacagtaaa agctgtttct gttcttcatg
     7381 aagcaggcgt gtggtattgt ccaggaaaag ctgctaatgc tggaggagta gcagtatctg
     7441 ctttagaaat gagtcagaac gtccagcgat tgacttggga aagagagcaa gtggatgagc
     7501 agctggatca aatcatggaa acgatttacc atacgtgtcg ccaaacagca gccgagtacg
     7561 gcgacgaaaa aaatctgctt cttggagcaa atgttgctgg atttgaaaaa gttgccaaag
     7621 caatggcagc agaaggttta gtttgaaaaa tgaaactttc ttggaaatat tgtcagcatt
     7681 ttgtttattt gcttgaaaaa taataaaaaa aaacgtaaag tattacctgt agagtaaacc
     7741 tataaggagg agaacaaatg gcacacattc attttgacta ttcaaaagta gcaccgtttg
     7801 tcaacgaaca agagttaggg tacatgcaaa gccaagtaac agcagcccac aacgaattac
     7861 gggaaggtac tggcgcagga agcgacttcc gcggctggat cgatttacca acgaactatg
     7921 acaaagaaga atttgcacgt atcaaagaag cagctaaaaa aatccaatct gattctgaag
     7981 tattagttgt tatcggtatc ggtggttctt atttaggcgc tcgtgcagcg atcgacttct
     8041 tgaatcacac attcttcaat ttattagata gcaacacaag aaaagcacca caaatcttct
     8101 ttgccggaaa ttcaatcagc tcaacatata ttgctgactt gatcgaagta atcggtgatc
     8161 gcgacttttc agtaaacgtt atttcaaaat ctggaacaac tactgaacca gcgattgcgt
     8221 tccgtgtatt caaagagctt cttgttaaga aatatggaca agaagaagca aacaaacgta
     8281 tctacgctac aacagataaa gcaaaaggtg cagttaaagt agaagcagat gcagaaggtt
     8341 gggaaacttt cgtgattcct gatgacatcg gcggacgttt ctctgtatta actgctgttg
     8401 gtttgttacc aatcgctgtt agtggtgcag atatcgatgc attgatgcaa ggtgccgctg
     8461 atgcaagcaa agcatactca agcgacaaac tagaagaaaa cgaagcttac caatacgcag
     8521 caatgcgcaa cattctttac cgtaaaggca aagtaactga gttgttgatc aactatgaac
     8581 caggaatgca atacttctca gaatggtgga aacaattgta tggtgaatct gaaggaaaag
     8641 accaaaaagg catctatcct tcaagcgcaa acttctcaac tgatttgcac tctttaggac
     8701 aattcatcca agaaggacgt cgcaatattt ttgaaacagt tgtgaaggtt gaaaaacctc
     8761 gtaaatcaat cacaattcct gaacaagcag aagatttaga cggtctaggc tacttgcaag
     8821 gaaaagaagt ggacttcgta aatacaaaag catttgaagg aacattgctt gctcatacag
     8881 acggtgacgt gcctaacttg ctagtcaaaa ttccagaaat ggatgcttat acattgggtt
     8941 atacaatgta cttctttgaa attgctgtag gtatttcagg ttacttgaat ggaatcaacc
     9001 cattcgacca accaggtgta gaagcttaca agaaaaacat gttcgcactt ctaggcaaac
     9061 caggctttga agatcttgct aaagaattga acgaacgcct gtaagataag ggtttttcgc
     9121 tagatttaaa taatcagtga tagaaacatg ttcgctttaa aatgaaaact ggtacccatt
     9181 taggtactca aacatactgc atcgtttatt cagaataagg ggctgtgaca agacgtttgt
     9241 cacagtccct tgtttgtata gaaatcgatt gattgccttg ctctttagtt tgttttagga
     9301 ctttaagaac agtgaggctg acttcgaagg ttaattctgt ttttttgttt tctagaatca
     9361 gtaagcaaaa aagcactcac ttcgagtgct tttttgcgat atattcttag cttaagatag
     9421 gcctaatttt tcttttacag atgagggtac cttatcgctg gtaattactt ttttctctat
     9481 gatccgcttt tcgcttgctt tcaactgcaa gatcgttcct tcggggtaag cagaaccatt
     9541 ggcagagtca ccagaagtaa tgacatcgaa atctaactgt cttttttcac cgttttcagc
     9601 ataagcatct acgttgtatt taaaacctct tccaattact tccccattat ctgctttgat
     9661 atcagtttct gcaggcattg gagctttgac taccgcataa taatctttac ctacataagt
     9721 agactgatag tagtcccaac ctttccatcc tataaaagca atcaatatga taacgatcaa
     9781 ccctaaaatt tttttcatga aagatctcct tttttatttt tat
//
LOCUS       DACDPS010000085         9770 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.085, whole genome shotgun sequence.
ACCESSION   DACDPS010000085 DACDPS010000000
VERSION     DACDPS010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.085"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            119..313
                     /locus_tag="IWJ07_002261"
     CDS             119..313
                     /locus_tag="IWJ07_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP9333802.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            316..1518
                     /locus_tag="IWJ07_002262"
     CDS             316..1518
                     /locus_tag="IWJ07_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9333803.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(2101..3732)
                     /locus_tag="IWJ07_002263"
     CDS             complement(2101..3732)
                     /locus_tag="IWJ07_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAP9333804.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(3746..5419)
                     /locus_tag="IWJ07_002264"
     CDS             complement(3746..5419)
                     /locus_tag="IWJ07_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9333805.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(5432..5749)
                     /locus_tag="IWJ07_002265"
     CDS             complement(5432..5749)
                     /locus_tag="IWJ07_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP9333806.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(5774..7174)
                     /gene="lacG"
                     /locus_tag="IWJ07_002266"
     CDS             complement(5774..7174)
                     /gene="lacG"
                     /locus_tag="IWJ07_002266"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP9333807.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            7411..8196
                     /locus_tag="IWJ07_002267"
     CDS             7411..8196
                     /locus_tag="IWJ07_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9333808.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            8199..8891
                     /locus_tag="IWJ07_002268"
     CDS             8199..8891
                     /locus_tag="IWJ07_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9333809.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(9165..9536)
                     /locus_tag="IWJ07_002269"
     CDS             complement(9165..9536)
                     /locus_tag="IWJ07_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP9333810.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
BASE COUNT         3101 a         2065 c         1708 g         2896 t
ORIGIN      
        1 gagttgggaa gtcctgaaca agtgatggat tttcaagaaa gtaccgttca ttatacaaaa
       61 ataaagaaat aacactatta cccaatgata gtgttttctt tgaaagtgag tttatacaat
      121 gaaaaataca atttgtcgaa aagatttaga ggctctagga tttaaaacac atcaagctac
      181 cacaataatc cgacaagcaa aaacaagaat ggtaaatcag ggatatccat attataataa
      241 taaaagagtt ggggtagtac cccgacaagt aattgaagaa attataggtg ttcctctttc
      301 acaggaggaa taactatgaa aacaaaatac ccttgtgtgt atcaagataa aaatggtcgc
      361 ttttcttatc tagttgagtt aggaacagat caagtgactg gaaagaggat ccagaagaaa
      421 ggaacaaaag attctctagg aaaacgattc tcatcagctc gtgaagcaca caaagaagtc
      481 atgcgtataa aaaatgaata catggttgta aatggatttg cgaattatga tttaacttac
      541 aaagaattta tggagcagtc ttacgttcca cactacaaag ctagcgtaca aaaaagtact
      601 tggagttctc gtgagtgtgg attgattcag attactgaat ttttcggtga taagaagctt
      661 cgtgaaatca atgtgcgaga ttgtgaaaat tatcgaattt ggttattgaa cgatagcgga
      721 tactcacaag catacagttc acttatgtac ggtatgtttc gtaaatcatt agactatgcg
      781 gtcaccctgc aatttctgaa tgagaatatt tccaaaagaa caaaagcaat tccaaaagga
      841 aaatcaatcg ttccttactg gacaaaagaa gaatttgaag aagttttggc aaaaatttat
      901 aaagatgatt tctacgaaca tatgtgtttt ttaatgatct ggctatatta catgactggt
      961 gttcgtgtaa gcgaaggatt agcacttaat tggaatgacg tggacttcaa acgtaaaaag
     1021 ttgagggttc atcacacctt ggaaatgaca aatcaaaaag attttattcg gaaaccttac
     1081 acaaaaactg aaagcggctt gcggatcatt tctctggacg atgatacaat tgaagtgcta
     1141 aaaaagtgga aagttattca aaaaaatcat ggtgtagaac aatttattct tagctacact
     1201 gacctcccac tctatcgttc aactgttcaa cgaattattc agcgttatgc caaactagct
     1261 aaggtacccc taatacaagg aaaaggatta cgtcattctc atgttagcta cctaatcaat
     1321 gagtttaacg cagacattct ggtcgtgtcg caacggttag gccattctag ccctgaaatc
     1381 actttaaagc actacgctca tctatggagc cgtaatgatg aaggaattgc agaacaaatg
     1441 acaggaaaca ttaaatttac ctgtgcaaca aaatctaaag ttgcaaactt taatggaaat
     1501 caagcagtaa aaatctaacc ctaccatttc cctaccaaaa tgaggaatag gctgaaaaag
     1561 cactgatata atagcttctt tacgaggcta ctcatattga gtgggaataa agagccaaca
     1621 taatatggag ggggaatccc tataaatcaa cattcttcaa tagctaaaat gcttgatttt
     1681 cattaaaaat aagatggttc cctaccaaaa gagactataa tataagttaa tttgtttcat
     1741 tcttatcata aaaagaagtt ctcgaaaaac gaactgtttt ccgagaactt ttttctatcc
     1801 atctaagtgc tcaaatcata ttcacgacgg aattctaagg ttaccctttc ttaaacaaac
     1861 tttatgttat ctgcacaact ttttgcaatt ttttcattgc ttcttacgaa atcgttgaac
     1921 tctctttagc ataatacaaa gtgtagatga agtctggtgc tttactcgtt ttggtaaaaa
     1981 tgactggaaa tcgtttctgg ttatttttgg caaaggcgag gtgcaggttc gattcctgcc
     2041 ttctacgcta acgatgtggc tgaagggaac ctccgatctt gaatcgaaac gatccgcagt
     2101 ctattgtttc accaatgaaa caaaatcaac gttcgtccaa gcatttcctg aagccttgct
     2161 ataaacccca atttcacaaa cacctgttgt aattggaatg tctttgattg taatttttgt
     2221 ccattcactt gttttaacaa ccgcagtatt tagttccgct ccaccataat ttttgatata
     2281 taactgatta gtatcaaact ctcccccaga cattacatag gcactcaatg tgtaagttcc
     2341 tttgcttatg ccactaacat tttgataagc tgatgcctcg tatgcttgat cctgccaaaa
     2401 agttagtttg tagtccccaa aaaaagtgcc cgcttccgtt ttgatcgtac ccttagcggt
     2461 tccttgttga aaccaggttt gccaattgga aggactggtc gttggtccat cgctttcgaa
     2521 acttgcattt ttaactagat ttatttgcgc ccctgcttgt ttaaacgcac gcaagcttgg
     2581 caaggcgttt ccagcaaaat caaacatcgc ttggttgtcc cattcgtttc ccatagtgga
     2641 ttgaacactg agataattca tacctgcttg tgttgcccaa ctcacatttc cgttgtacca
     2701 aagaggttcc caatagaaga agcccgcccc gcgattattc ggtactgcct gaatccgatc
     2761 ggcaagatcc agcaaatact ctgcttgtcc tgtcggtgtt ggttcatatc cccccgcagc
     2821 tgcttcggct tcaccaaaag catttggcat agtatcggca ttcgctgttg tatgcccata
     2881 agctgtttca actacgatca cttctttacc ataccgttgg ctgatcgcat tcatattggt
     2941 ttgaagttca ttcattgaac cgtgccaata cggataatac gacactccaa ttaaatcaaa
     3001 tgacacacct cgactcgtga tttcatcaaa ccaccaagta aatgctcctg tatcgccacc
     3061 atcagctaag tggagcatta ctggagttgt tttaggttga gtgtctcgaa cagcttggat
     3121 accagacttc aaaaaagcag ccaatcgatc aaattctccc ccgccttctc cccaagactt
     3181 gccataaggc cagagcattc cgctattcag ttcattgccg atctgaacca tatcaggata
     3241 aacgtccagc tgcttcattt ccgacagaac gttctttgta tagtcgtaca atgccgtatt
     3301 catttcttca aaggtcagac cttgccatgc tttaggaaga ttctgtttcc caggatcaac
     3361 ccaaaaatca ctgtagtgaa aatccagcag caccttaagt cccagatttt ttgctcgttt
     3421 tgcaaggtca atcgtcgttt ccagatcatt tgtccctgct ccataacttt ctccattact
     3481 attataagga tcctgccaca aacgcagtcg aacatagttg acaccattat tttttaaaat
     3541 ctctaaggca tcttttgaaa tatcgtcttc ataatatttt gcaccagatt tttccatatc
     3601 tgacaggatc gaaatatctg ctccgcgaat aaattcgctg gcagatgctt caacagtttt
     3661 tgaaacagcc cccaaagccc ctaacacagc tgcaaataaa acagctttac gcaaccaaat
     3721 tcccattttc attatggctc ctcctttatt gaaattggtt cagaataaac tgaacagctt
     3781 tttcaggatc tctcgttaat ccgatatatt ctgctccttt tgtcgcaacc agcttgattc
     3841 ctaagcgatc ggtgtcttct ttgatatcat catagtaaga attgacttgc ggtgctaaga
     3901 ccaccatatc aaaatcctgc agcatttcgt aatgagaacc ataagcaccc gccattgcac
     3961 tgatcggtgc tttcgattca gatgcacctt tttttaaagc attagctaac atcgcacttg
     4021 tccctgcgcc agcacaaagt accaacacat tgatttcttt atttgcatcg ctgataactt
     4081 gactcgctgc cacaggtact tcctcacgta catcggcaga agcaccttcc tcggttgcga
     4141 tcacctctgc tttttgtgct tctttctcta ctaagacagc atcgtatgct ttgcaaaatg
     4201 gtagatagat caggaagtca acgacgatta gaaccgctgc tagaacaaaa gctaagatac
     4261 ccatgcccgt tcctagaacc aaaccgatcg gtgctggagt tgcccaaggc aaaacgtaca
     4321 taaagctgtt catgcccaat acatcgacaa aaaacttgaa caggcaaacg tttacaacag
     4381 gtgttaacaa aaatggaata aaaaaataag ggttcaaaat catgggtgcc gcaaatagca
     4441 atggttcgtt gacagcgaaa cttactggaa taaaggaagc tttcccaact gccttcaact
     4501 gcttagactt agcaaagagt aggaagataa aaggaacaac caatgttgcc ccagttcctc
     4561 ccatagttcc tacaaaattc cctaaaccca ccgtcagtac tttagaagct tgttcaccgg
     4621 aattgaacaa ttgcagattg gtttccacat tggcatagat aattgccgca attgccggct
     4681 ccacgatcga cggtccatga acaccgacaa accaaaacag cgccatcgct ccccagataa
     4741 tagcgattcc taagtacccg tcagctgctg taaatagcgg ctgcagcacc gtaatgattg
     4801 cttctgcaaa tgagatttgg aagatttgtc gaatgcctaa atcaattaaa acggccgcaa
     4861 atactgaaaa agagaaagga aaaatatctc ggaaggtttg cgaaatcgtt ccaggaactt
     4921 ctttaggcat tttaatggta atatctttca gcacacaaaa cttatataaa ttaactgtta
     4981 aaaatgcagc gacaaaggaa gccagtagtc cctttgtccc catatattcc gttgaaaatc
     5041 caccttcaat cggagagacg cttaacaaca ggaaacttac cacagaggcc agcatcacag
     5101 aggtggtgtt gattactttg tttttcggta agcgacgatt catcgaatca gccaagcagc
     5161 gagcagtggt tgctgaaacg agtaacccta caacacccat cgaataatta tagactttcc
     5221 atagccatgt ggagacagat tcaggccatt ggtaaccaaa aatctcagga accgcagcaa
     5281 tcaaaatgaa aatgcttgaa aataaaatag ctggcatcgc tgttaaaaat ccgtcacgaa
     5341 cggctcctaa ataactatta cgtgagattt tttcaaaaaa tggtttccct ttttcgattt
     5401 gtttaatgat tccatccata gcgttcttct cctatccttg attctgtctg tacagtacaa
     5461 taaagtcttg aatcagatct ctcagcaata aagtggtcat taaatgatcc tgcccatgga
     5521 taaaaatgaa tcccatctcc atctctttcc cagatgcttc ttccgctaaa atttttgttt
     5581 gcgaattatg tgcaagggtt aaattttcgt cggcttcttt caacgccgaa tcaacatgtt
     5641 caaagtttcc ttgtcttact tctttcaata atttcaataa tgtactgcga gcgtcgcctg
     5701 aatatgcaac gatctcaaaa ccaagcattt gtaattcctc tttattcatc ttttcttcct
     5761 ccattatcac ttattaaata atcgttttgg tttcactgac caacttgtac cagtaagctg
     5821 attcttttgg gtaacgcttc tgggtttcaa aatcaacata aaacaagcca taccttttgt
     5881 tatacccatt tgtccatgag aaaagatcca taagcgacca caaaaaatac cccttcacat
     5941 ttaccccggc tgttatcgcc ttgcttaagg attccaaata gactctcaaa tagtcgattc
     6001 tcggctgatc cataataatg ccatcttcaa actgatcttt ataccccatc ccgttctcag
     6061 tgatatagat tttgttgtaa tgtgggtaat cgcttttaat tcgcagcaac aagtcatata
     6121 gaccttctgg ataaatcagc cagtcccaat cagttcgagg aattccttct ttataaatac
     6181 gttctccgat tcccttcact ttataaacag aggtcccttt ttcacccgta ccgttatgat
     6241 ggattgcatt ttctccatcg taagctttga caaaatggca ttggtagtga ttgatcccta
     6301 agtagtcatt acgcgtggat gcttttttca attcaacgaa atcttcttca ggaaaatgat
     6361 agctggcctg atttgcttca catatctcat caagagcagt caaggtttca gttgaataat
     6421 agcctaaata ggtagcatct aataagaaac ggatcgacaa tgcatcgtct aaaaaggcag
     6481 catgcttatc ttccggcgcg tctgttgccg catatttcgt ctctaatgag tgaactacac
     6541 caatttcacc cggaagttcg ttttctttga aatagttcac tacacgggca tgagcaacca
     6601 tcatattatg aagacaggcg acgatctttg taaaatcata tttgattcct ggtgggaaaa
     6661 cacctaacaa atattgattg gttgcaactg gatagatttc attaaaggta ctccacacct
     6721 tgacttcttt gaattcatga aaacaaaaaa tggcatagga aacaaatgct tctattgttt
     6781 ctcgattcaa aaaatctcca tggtcaaaca aacgtttagg ggtatcaaaa tgatgcaacg
     6841 tcacaaacgg cgttacgttt cttttcttac attcttcgaa cacacgatga taaaaagcga
     6901 ttccttctgg atttggtttc ccagctccat caggaaaaat gcggctccaa gcaatggata
     6961 atcttagacc atttacacca aaacgttcac acaattcaat gtcctttgca tattgatgat
     7021 aaaaatcact tgccggatcg ggactgaatc gcccttgttc ctctaaaaag tcatcccaag
     7081 caacagcgcc tttgccacct tccttagtgg ctccttcaac ctgataggct gctgtggctc
     7141 cgccaaaaat aaaatcttct ggtaatttaa gcatgtacat ctctcctcaa atctataatc
     7201 agttttcgct ctttctaact gattgcattg taaacgcatc caaaacattt gtcaacaaaa
     7261 acaaacacaa ataatcataa aaaccagaaa acagcaataa aatctattgt aacaaacata
     7321 aaaaccaccc taaatgttta cttttgttcg aattagattg actgttgtcc gtttatatga
     7381 tagattatcc ttaaattaga ggtgaagaca atgttgaaaa aagaaagatt gcttactatc
     7441 gtagaaatgg tcaataaaaa aggaatcctt accgttaatg aaatcatcaa tcaattagat
     7501 gtttctgata tgaccgttcg cagagattta gatgaattag aaaaatcggg caagctgctg
     7561 cgtgtccatg gaggggctca aagtctttcc tacactttag atcaagaact ttctcacact
     7621 gaaaaagcca ctctgcagat tcaagaaaaa aaagaaattg ctgccacagc agcctccttg
     7681 attcacgagg gagaaacgat tttcttagga ccaggtacaa caatcgaact attggcgacg
     7741 catttactga acaagaaaat tcggatcatt acaaataatt atccagtttt tgatattctt
     7801 gctcaagcag atggaccaga aattctactg attggcggtg atttccggaa aaataccggc
     7861 gcatttgtcg gaccgatcac caacgacaat ttaagaagac taaagtttac aaaagcattt
     7921 atcagttcaa atggcgttca caacgaagcg atctctactt acagtaccga agaaggagaa
     7981 gcacagcaga tcgctctaaa taattcccgt aagaaatatc tactcgttga taataaaaaa
     8041 ttcaatcgag atgattttta tgtgttttat aatttgcatg atttcgatct gctcattacg
     8101 gatcgccaga tctcgcggga ggttcaagag cactatgcac aatatgtcga tctgcaaatt
     8161 gctgattcaa ccgaaaatac cactatagga gcgtaactat gaaagggatt atatttgatt
     8221 tcaacggaac aatgtttttg gactctcatc tgcatgaagc tgcttggctg catatgatac
     8281 atgatcatac aaaagatggt ttatcagacg aggatatttt aaaaaatatt catggtcgga
     8341 cgaatacgga gattttgaaa catttcattt cagagcattt gaccgcctct gagatcgccg
     8401 tattgtcaga agaaaaagaa agctattatc gtatgctttg cttgcaaaat gaaaacgagc
     8461 ttcagttgac aaaagggctt gaagcgactc ttgaccagct gactgaccaa cagattccat
     8521 taacgattgc cacggctact gtcaaagaga atgttgcttt ttattttgat attttcgatt
     8581 tagcccgctg gtttgactgg gacaagattg tctatgacga cggctctttc cctgggaaac
     8641 cgcaaccgga tatttttctt aaagcggcag acaaactgag cttagctccg gctgattgtc
     8701 ttgttatcga agatgcttac tcaggattac ttgctgccaa acgtgcgggg atcggaacaa
     8761 ttattgcaat tgatccattc ggtaaaaaca aaacagtatt tgaacaggca cagctaaata
     8821 aagatggaac catcaaagat tttactggtt tttggacaga gcacctgtct gccacatccg
     8881 ttaattctta aacgattcaa ctaactacca aagaaaagca actgaaatgc acacctcctt
     8941 ttataaaaaa ctgccagtga aacgacatga aagatacact gctatatcct tagtgaaata
     9001 tagcagtgta tctttgactt tttcattctt ttaaccatat gacgcgttca tctctatcta
     9061 taattaacat taaaaatagt gttttcacat tatttctcaa accaatctta aaaatcatca
     9121 ggagtacttt tttgatatac ttttattaat gctgggcgta atggttaaaa gatttccact
     9181 agttcctctc caccaagctg agttgaaaaa tgcttatctg taaacaacat agacaaagag
     9241 gaaataaacg tttccgtatc tttctctgtc ccaaggatat taattaattt tcctactagg
     9301 taagtatcat taaacttaag atctgccttc acatcaaaag ttttgataaa agcttttatc
     9361 atgcgtacaa ttttgtcttt atcgccataa ttgatttcac cactcatatc aatatagtca
     9421 ctaatccctg aagaaataat ggcaactgct tcttctttcg ccttatcaga taattcattt
     9481 aatgactggg attgattgat gacttcttga gttttatctg ttacgtaatc tctcattatc
     9541 ctatcggcat actccgcaaa gtaatcttga aaaaattctt ctacagggta accaacttcg
     9601 ttttcccaac caggagaaat ttcattatca gtacttatta aatctattaa aaaccaaccc
     9661 gttctctgag tgttgattct ataatatttt taatttccta tataaatcaa aaatattata
     9721 gaatcaataa gctaattaag aaacaaactt tagaaactct agaagaactt
//
LOCUS       DACDPS010000086         9695 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.086, whole genome shotgun sequence.
ACCESSION   DACDPS010000086 DACDPS010000000
VERSION     DACDPS010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.086"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            311..1546
                     /locus_tag="IWJ07_002270"
     CDS             311..1546
                     /locus_tag="IWJ07_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP9333811.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(2545..2805)
                     /locus_tag="IWJ07_002271"
     CDS             complement(2545..2805)
                     /locus_tag="IWJ07_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP9333812.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2832..4133)
                     /locus_tag="IWJ07_002272"
     CDS             complement(2832..4133)
                     /locus_tag="IWJ07_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9333813.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(4425..4883)
                     /locus_tag="IWJ07_002273"
     CDS             complement(4425..4883)
                     /locus_tag="IWJ07_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP9333814.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(4896..6221)
                     /locus_tag="IWJ07_002274"
     CDS             complement(4896..6221)
                     /locus_tag="IWJ07_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAP9333815.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(6238..7650)
                     /locus_tag="IWJ07_002275"
     CDS             complement(6238..7650)
                     /locus_tag="IWJ07_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9333816.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(7667..8431)
                     /locus_tag="IWJ07_002276"
     CDS             complement(7667..8431)
                     /locus_tag="IWJ07_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAP9333817.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(8535..9410)
                     /locus_tag="IWJ07_002277"
     CDS             complement(8535..9410)
                     /locus_tag="IWJ07_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9333818.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
BASE COUNT         3143 a         2012 c         1468 g         3072 t
ORIGIN      
        1 cgtgtgtata atgacgacag cacacctatc aattaatata attattttat ttttttatgt
       61 tctcatgttt atttttaatg acacaacttc tcgtatttat ctggcacaac atataaatcg
      121 atcaaaaaat tgttgcgtgt ttttttgaaa attggagttt caacttgatt acaaaattta
      181 aatttagaag ttgtgaaaac agcgagagac agtttaatat tcaaaatata cataggctac
      241 tcgaacgctg tgttttttag tatactagta agaaacgaag ggatagactc attccaataa
      301 ggaggatttc atgaagacaa ttctttttgc tcctgcagta ttcaatttag ctgagacaac
      361 acgaatgatt gaggttgcca aagctatgaa agaccgttat tactgtgtat tctttggctt
      421 ttctaaaact tttgctcctt tgatcacaga aagtggcttt gagtatcatt tactagcgcc
      481 gatccttacg aaaaaacaag aagaacaaat cataaaattt gaccagttgc gtacacttaa
      541 aaaccctttt accaaagaaa tggttgcctc tcgtatcact agtgaacaga gtctgattgc
      601 taagtttgag cctcgactga ttgtgacagg ttcgaatgtg acaatttttt tatcggcccg
      661 caataaaaag attcctttag tatatgttaa accctatgct ttgagtgaac ctttttttca
      721 atcagagcat gcgctgatgc cccacgagtt acgtttcttc ggccctttaa gcaatcccct
      781 ttggcactgg acaaaaaagc gtatagtaaa tacccgatgg atccccaatg ccttcaaaca
      841 cgctgcacaa aaagaaatgc ttcctttacc aaaaacaagt ttacaaatga tggatgctga
      901 tctaaatctc atcacaacgc ctaaactatt tttggaagaa gaacagctgc cggaaaatta
      961 tcgaatcgtt ggtccaattt ttgctaagct cagtacttcc ttgcctgaag aagtgctaac
     1021 ttttatacag cgaaagcaac aagaaagaaa acgtattatc tatttcgcaa tgggtagttc
     1081 aggcaatccg cgtatgttac gcaaaatttt gttcttctta caaaaacaac cggatctagc
     1141 ggttattgca cctattgact atctgcttga tgacattcaa tctgactcag aagatctttt
     1201 aataacagaa tatctgtcta gtcatctttt gcatgaattc attgattttt cttttatcca
     1261 cggcggggaa gggaccgtcc aaactgcctg tgaatccggc aaaccgttta tcggtgtggg
     1321 gatgcaattt gagcaaagct gtaatattga ctattgtgta aaatacggaa acgcaatcgc
     1381 cctcacaaaa ccagtgactg aaagaaaact gcagattgct ctgcgcgaag tttcttcacc
     1441 caagattcgt cagcaggcac gcacgctgca agccgttttt ccaataaatg gtccacaaca
     1501 agcaattcaa gaaatcgaaa ccttcctgag caaccaccca atatagtttc aagtagaaaa
     1561 agctttttta atagagactc atcagtaatt tcgctattcg agacccagac agtgaaaata
     1621 caaaaaaagt aaatagtgga ttgaaaagtt agagttactt aaattacggt gtctaaaaaa
     1681 cacaaaatca tccttaaaat aaaagatctg acgctatcca aatgagcata ggtagcgtct
     1741 gccatttatt cttagtcaaa ttctttgaca cctttctctg gttggatcat ctcaacgttt
     1801 cttcaaaaga aagaccataa taattaggca aatacatttt cttaaaatgt gtcatataga
     1861 tctagaagca tcgctttgta gagatttgtg ccgctagtcc cctcaacaat tactaaatca
     1921 aatgtaggat ttaccacttt atctttgata atcaacctcc ctcttctgtt gagacaatga
     1981 tctcgcaatc cgatgcatac tcctatataa actgccagca atccccacat attgagtaga
     2041 tcatgtcaga atgttctatg tcgtcagcaa cgatacacta aaagtcacgt tcaccatgac
     2101 tgattgctat tcccagcaca attatttcta tacacgtttc atgatttgaa taaacactca
     2161 catcatacat attaccattc atacaccata ctgcagcccc tactatatgg ttgtaagctc
     2221 ccaaatctat atttgacttg atcactttgg tagtagatct actaattcta aatccattgc
     2281 tgataaaatt tccatttttt cctccttctt tctgccaaaa cagttaaaac aataataatc
     2341 tttatgtaaa aaagggctgt gacaaaagcc ttgtcacagt cccatattcc gaataaacgg
     2401 tggtcaaaag cttgcatctg cggtaataag ttcaaccaaa cgaaaaataa gccaacattt
     2461 cacaaaaatt taagaagcaa ttttcgtaaa tttcttctta tttcttgaag ctaaccactt
     2521 ctgttacaac cacttttaga acatttaaag cttcgatgtg acaaaccatc tatgctgctc
     2581 gatcataaaa ttgatagcaa aaattccaca aagtacatat gtcccccatg aaaacaaaac
     2641 attcatatgc ggtacaatga tctttggaag aatcacgatc aaaaatgatg ccagtgccca
     2701 aatccatcga ttgatttgaa tatgatatgt agctagcata ttcaatataa ggtgactagc
     2761 cgttaataaa aagataaaaa caaaaaataa aatcagccaa tacattgttt cccaccttct
     2821 aaaaaccaaa cttagacagt ctggacttcg ttttctccac gactttgttc atctgacagc
     2881 atctttttat cataagcttt tacaaatgga taatagataa taaatgctat caatgtgttg
     2941 atcatgatca agataacaac acgccagtca tttcctgctg aaagataacc gccgattgga
     3001 gctggtaatg tccatgttgg caaaacagta aatctattga ccatttctaa tttgactgca
     3061 aaataactga taatagtcaa aactaaagga actaagttga acgggatgaa catgtaagga
     3121 ttcaacatca taggagctcc aaaaattatg ggctcattga tattgaagat accaggaata
     3181 atagaaaaac gaccaacctc tttcaagaat tttgatttac agaagaatat aaacaaaaca
     3241 gataccacga tcgttgctcc tgcaccacca attgttaccg tccattgata aaactgttca
     3301 ggagcgatat agggtaattg ggtggcagct gtaccatctg ccaatgcctt tgcattctca
     3361 tccaacatga tcagccacat tggacgaaca acagccccta caatactcat gccatggata
     3421 ccaaatgacc acaacaaatg gatcaaaaac attggtagta aaacaccaag caaattattc
     3481 cctaaaatat tggttaaagg ctcgaataac gataataagg cagcgtttac atcaaagccc
     3541 gcgacgcttc gaatcagcca agtaattgtt aaaatgactg cacctggaat caatgcctca
     3601 aaactacggg caacagcagg tggcacttgt tctggcattt tgatagtaat atttcgattt
     3661 ttaaagaaac gatatacttc cactgcaaaa atcatagata agatccccgc aaacatccca
     3721 gatgctccca aagaactcat aggtaagacc cagccaagag gtgcctcttc aggagttaaa
     3781 acgtttacag gaacttgtaa cataaagaac gttcctaaag ataatatacc acctgtcaca
     3841 ctatctaatc catatgattt agctagatcc gaacccataa caaaagttgc ataaatagcc
     3901 atcaatccca tcgtcatcct aaagggtaaa acaatcgttg ccgaataagg ggcaataaat
     3961 tcattccatc caggaaacgg taaattgata aagaccataa aaaatgtccc aatcaaaatc
     4021 aatgacaatg ttgaaacaac cccattacga attgctaata aatgacgctg tgtaccaatt
     4081 tttgccattg gtgggacaag tttctcttcg acccatttca tcagcttttc cactaaaacc
     4141 actccttttc aataaaatat gaaccattca ttgcatgaca acgcaaagaa tgttcataac
     4201 taattatata attaagaaat tttatttcaa tatattctct taaataatca aatttatttt
     4261 taagaatcct gaaaataagt tttctaaatc ataggagcaa caaaaaaatt cgataattaa
     4321 caaaaatcaa aatgattaaa tctagtgatt gaaacatatc atttcaaaaa aattttgtaa
     4381 attcacccat aaatatcacc ctatttaact tttttcatcg tacactaaaa ttcaaaacaa
     4441 ctatctttaa tctttacttc ttggttttgt tttaataaat ttccttcaaa aatgcctgga
     4501 gaaactacaa tgatcaccgt atctttttgt gcttgctcca acttcttcaa atctataata
     4561 gctaacagtg tcccttcttt tacctcctgg ttactttcaa cccgtatctg aaacggactt
     4621 ccttcgagtt caactgtatc taaacccata tggatcagta tttttctgcc agattcactt
     4681 tcgatcgtta tggcatgtcg ggtaggaaat atattatcga ttattccttt aactggcgca
     4741 tggatatatc catcatgatt cgttatagcg tatccaactc ctatcatttt tgaagaaaag
     4801 acctcgtctt ttactgctgt taatggaata atttgaccac tagctacagc ttttagaatg
     4861 tttcttgttt taaagaaatg cactttttat cacctctact ttaatgctgg ccaatatttt
     4921 ttgtttactt tgatcatttc atctagtatt ttttttgcga ctctggcatt tggtacggtt
     4981 tgattcaatg taaatgcttg taaagctttt tgataagaac cttcaaagta agcatctact
     5041 agtaacttct ctactgcaac ttgtgcctcc atcaatcctt tatgaaaatc actgattggt
     5101 tttcttagtg taattggctc cacaccagtt gcaccaacat atgccggtat ttcaacaact
     5161 gcatctgacc gtagattcgg aatcgcccct tgattaggaa cgattagcat aaagcgacga
     5221 cgatcatcat tcaacaagga aatagctata tcgacgatat attgtccatg ctccccaaaa
     5281 ttaaagttca atacttcttc cacaataccg cggcgtattt tatcagccat ttctttggta
     5341 tttttctctc gtccatccat gaccatatta gcacgtgtat actctttgtc agtatgctca
     5401 acgaccatat caggatataa gtaatactct aagtaattat tcgggatatt acttggaaaa
     5461 tttttcgtga tgactgacat catttgaaag gttttttgcc aagttttatc tcctatatta
     5521 aaatcggcga cttgcatttc ttttgttatt agtttttcaa taatctctgg cataatttct
     5581 cgttgcaatt ctttatcata aatcgatgta taccagccaa agtggtttaa accataatac
     5641 gtaacgatcc aattcttacg atcataaccg taattcactg caatcgtttc ttcaattgaa
     5701 atagtcatat cacaggcatt aatcattttt acattcggat atttacgacg aacagcttca
     5761 gaaacgatcg actctggatt tgtataattc aaaatccatg ctttgggggc atagtcttgt
     5821 atatgatcga caatttccaa taaacctttg attgagcgca tcccataagc aaatcctccg
     5881 agtccacacg tttcttgtcc tactaatcca tgtttaagtg gaatcttttc gtccttttct
     5941 cgcatctcca atcctccaac acgaatttgt gagaaaacaa aatcgcatcc tgtaaaagca
     6001 agctttggat catccgttgc acgaatgcga atatttgaaa aaccttttct tttcagcata
     6061 aatttaacaa tcaagtacat atccatattt ctttcttcat caatatcata taaacgtatt
     6121 tcagataatg gcaaccgttt ttcattgttc aaaatggctt gaacgattcc tggtgtatac
     6181 gtactcccgc ctccagctat agtgataatc tgttttttca tcatcatctt ttcctttcta
     6241 ttcttctaat aatttattga attctgccat aacataagtc acttctaagc cgatgataat
     6301 ttgataattt ttttcagttg gtttaatgat cccgcttatc cctaattgct tgattcgctg
     6361 ttcctcgacc atggtgggat ccactacctc tacccgtagg cgtgtattac aactatagac
     6421 atccaggata ttggcttttc ctccaaacgc ttgtagcatt tttgttgcca tatattcata
     6481 atttttactt gctaacccta actctgtttc ttctaaggtt acttcattgc caaactctgc
     6541 cttagcttca cgtcctaatg tctgaatgtt tctcttattg atcaaataat aaaaagtaac
     6601 gtaatacaag gcaaaaaaga aaattccgat tggaagaatc aaaatagcat tggtagcgat
     6661 tcgaaaattg atgagataat caacaataca tgctccgaat gtaaaaccaa ttcgtatatt
     6721 gaataagtaa cagacaatcc ctgctaaacc ggcaaaaagt gcatggatca cgtacaacat
     6781 aggagaaaga aacatgaaac taaattcaac aggctcagtg atatttgcaa taaatgaagt
     6841 tcctgcaccg cttcccatga ttcctttgac catctcttta ttttctttat aagctgcttt
     6901 atagatagct agacatatag ctggtatacc aaacatcatt actacaaaaa aaactgataa
     6961 aaacagtcca gcctgaggat ctccgttgat aaagcgtgtc atctctcctc tgaaaacttc
     7021 cttgctagga gatgtatagc ttcctaaatc gtaataaata tacgtattca acacatggtg
     7081 cagtccaaag gggatcagca aacgattcaa aaacataaat atccccacac ctaatgctcc
     7141 catatcaacg agcagttttg caaaattatc aatcccaatt tggatgagcg gccagataaa
     7201 gcccaaaaaa acagctgcga ttgtttgtag aatcatcacc atagtcaatg ggaatttttc
     7261 gcccgcaaaa taagaaaaaa ctactggtaa tttttgttct ttaaagcgat tatacgtcca
     7321 tgccgctact aaaccggaaa taattccacc aaaaatcccc atattcactt ctgggtccat
     7381 aatttctaag ccttttttca aggtagcaat cgctaaaaaa ccagtaagtg caggtattcc
     7441 tttgtcctta tttttgacaa aaccgattgc aactcctata gcaaataaga tatccaagtt
     7501 tccaaaaacg gcatttccag ctgcaccaat tactgggatg ttcagcatat cgtcagctga
     7561 taaacgcatg atcaatcccg ctaatggcat cgctgcaact ggaagtagca tcgctcttcc
     7621 aagcttctgc acatttgatt taacattcat ttttattcct cctctattaa atatcctcat
     7681 atgttactaa ctcaatcaaa ggatcatttt tcactttttg agcaaatgct gaatgaatta
     7741 aaaattcgac ttggtgggta cgaggataca caaaacttga accatttaac aattcctgat
     7801 ccaaatacgc tgcgtgacac atgacttcga tcgttttatc ttctctcaat atcatgagta
     7861 aacttgccaa atattcttcc acactcgtat tgatttgtcg ttttttttca tcagtttctc
     7921 ctacgacatc gaaacaaggc tcaaaataat cgacatgacg aacttcatct tttcgttcaa
     7981 agttgctccg aaccggcaaa tcatattttt ttgctaatgt aacaactacc tcctgatttt
     8041 catccaaagt atgagtatga tgatgactgt ctaaatgagt tggtatgatc cccgcatgat
     8101 aaattttttg aatctgagca ttccattcct gatacagttg atctttgtca atgttaaatg
     8161 gatgctgata ataagataag gatctgaact ctccgctttc gataagagta tcgacagttt
     8221 tgaggagtgg tttgccacac gtcaatgtta aatggactcc tacacctagc ctaggcattt
     8281 cctttcttag tttcacagca tgctcgaaac ccggcatatt tgccatcaat gtagttgatg
     8341 tcaaaattcc tcgctgataa gcatccataa tcccataatt cacaccatgg ctatagccaa
     8401 aatcatccga attaaaaatc acttttccca tttcattctc cttctttttt ctactaaaat
     8461 aggcgcaacc aaataatcat agtccattta ctatgtgtca tttggttgcg cctatagata
     8521 gttacgccct tattttaata gattcttgct agatgaattg ccaaatagat gatttcattt
     8581 tcttgaatgg caagttgaaa ttgctgttcg atgatatctt taatctttag tgctgtacaa
     8641 tacgctttgc tatgtttaaa tgtttcagta aaaaaaccaa tattttctac tggcgtttct
     8701 tcctgataaa tcaagcgttt aaccaggaat ttgatatgaa ccattaagcg atgataagtc
     8761 agtgattctt tatcataaac gtttgaatag gatttttcta gatgggacaa aatcgtggtc
     8821 gtcatttcta ctatttttaa gctctttaaa ctattaggct ctcctagctc agcattaatg
     8881 atatgcatag tgatgaatcc tacttcattt gaccctaact cggtttgata tcgatgattg
     8941 attcgctcga ccacttctac tgctatatca tattcatttg gataaaggaa tctcatttct
     9001 ggcaacataa ttgctgtgac atctctatct tctctttttc gttgaatagc aaaataaata
     9061 tgatctgtca atgtaatata gatatttgaa tctaatggag aggagatcct agctgttatc
     9121 agttcaatca cttcttccgc aaaatccata aattcgattg gaatctgcga gactatctca
     9181 ctgaatcgtt tgatcttttc tttagaatct aatacaaata atttgtcagc atcctcaaca
     9241 tcaaatggct tactcacttt agcagaaaag ccaatccctt tccccatcgc aattttttct
     9301 attccatcgt catcgatcaa tacagcatta tgattcagct ttttcatcac atacaccgtt
     9361 aattcacctc ctctatatga taaaacaaaa caacttgcag actccttcat acatattttt
     9421 gtctccaaac aatttttaaa gacaaaaaac gacctaagaa agaacccttt cttaggtcgt
     9481 gcctaattga atagtagcac cctacataat ttacaaaaat agtttaacac ataataaaag
     9541 cgttatcaat atctttttta ctcgttttat gataatactt gtatcgaatt gatttggagg
     9601 taattacata aaaacaaaaa aaatatagca actctctata agaaagttgc tatattgata
     9661 attataccgg cggccggggt cgaaccggca cgtcc
//
LOCUS       DACDPS010000087         9574 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.087, whole genome shotgun sequence.
ACCESSION   DACDPS010000087 DACDPS010000000
VERSION     DACDPS010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.087"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            181..1179
                     /locus_tag="IWJ07_002278"
     CDS             181..1179
                     /locus_tag="IWJ07_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9333819.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            1291..1800
                     /locus_tag="IWJ07_002279"
     CDS             1291..1800
                     /locus_tag="IWJ07_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAP9333820.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(1830..2489)
                     /locus_tag="IWJ07_002280"
     CDS             complement(1830..2489)
                     /locus_tag="IWJ07_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAP9333821.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(2491..3255)
                     /locus_tag="IWJ07_002281"
     CDS             complement(2491..3255)
                     /locus_tag="IWJ07_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAP9333822.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(3263..3940)
                     /locus_tag="IWJ07_002282"
     CDS             complement(3263..3940)
                     /locus_tag="IWJ07_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAP9333823.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(3977..5362)
                     /locus_tag="IWJ07_002283"
     CDS             complement(3977..5362)
                     /locus_tag="IWJ07_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAP9333824.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(5331..5585)
                     /locus_tag="IWJ07_002284"
     CDS             complement(5331..5585)
                     /locus_tag="IWJ07_002284"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333825.1"
                     /translation="MSDYNETTTDFRVCFFYFYSTVKMNVPDFLARLIYRILYLYNLL
                     QGRKNSEIELVSMEEKAAYGMIKIKRGERVHGKNSDFTYE"
     gene            complement(5572..5656)
                     /gene="ffs"
                     /locus_tag="IWJ07_002285"
     ncRNA           complement(5572..5656)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IWJ07_002285"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5679..6194)
                     /gene="tadA"
                     /locus_tag="IWJ07_002286"
     CDS             complement(5679..6194)
                     /gene="tadA"
                     /locus_tag="IWJ07_002286"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAP9333826.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            6301..7158
                     /locus_tag="IWJ07_002287"
     CDS             6301..7158
                     /locus_tag="IWJ07_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAP9333827.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(7239..7574)
                     /locus_tag="IWJ07_002288"
     CDS             complement(7239..7574)
                     /locus_tag="IWJ07_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333828.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(7641..9011)
                     /locus_tag="IWJ07_002289"
     CDS             complement(7641..9011)
                     /locus_tag="IWJ07_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAP9333829.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(9089..>9574)
                     /locus_tag="IWJ07_002290"
     CDS             complement(9089..>9574)
                     /locus_tag="IWJ07_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9333830.1"
                     /translation="MVYVEEYGMKSITARELAEFCGCSTYPIYTHFKSISGLKEKILE
                     EITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAKSDLDMDSLFKRPFFSYV
                     KGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWDKNEVTDEYLENILKEQS
                     P"
BASE COUNT         2806 a         1973 c         1615 g         3180 t
ORIGIN      
        1 gcatacttca taataataat aacataatac ctaacctctc gtaaaaatac tagatttgtt
       61 ttcttttatt tttcatcact agatacattc tataacttac cgtttcgatt tcgaattcaa
      121 acatttttga gaagcattct caattaaatt ctgctatagt gtgaaaaggg aggaattttt
      181 atggaaatct atgacattac aatcatcggt gcaggtcctg tagggatgtt tgccgctttt
      241 tacgcaggaa tgcgccaagc taaaacaaaa atcattgaca gtctacctca acttggtggt
      301 caattagcaa ctttgtatcc cgaaaaatat atttacgata ttcctgggta tccagcaata
      361 aaagcaagtg aattaatcga tcaattagaa aaacaattaa ctacttttaa ccatacattc
      421 catttaaaag aagaagttct ctcgcttact cgtgaagacg aagttatcga gattactaca
      481 aataaaggca tccattattc caaagcagtc attttagctt taggaaatgg ttcattccag
      541 ccaagaaaat tgaatttaga taatgctgaa tcatttgaaa accacggatt ggattatttc
      601 gtgaatgatt tgatgaagta tgctggaaaa aaagtcgcta ttgctggagg tggtgattct
      661 gctatcgatt ggacgttgat gttagagcct attgcaagcg aagtttactt gattcatcgt
      721 cgcccggaat ttcgtgcgca cgaacatagt gtatcacgct tgaaatcttc atctgtcaac
      781 ttgcttactc cctatctcat cgatggatta tcaggcaata atggggaact gacagatatc
      841 cggttaaaaa aagtgaaaag tgacgagacg attgatttga tgattgattc tctgatcgtc
      901 aattatggat tctcttctaa tttagaacat ctttcttctt ggggacttga tagtaccaga
      961 aatgccatta ctgtaaaatc agatatgtct acttctattc caggcgttta tgcagctggt
     1021 gacatctgta cctatgaagg aaaagtgaaa ctgattgcta ctgggctcgg tgaagcacct
     1081 actgcagtga acaacgcttt acactatatt aatccaaagg aacgaactca gcctggtcac
     1141 agcacaagct tatacgataa aaatcttcgt cctagttgat tttttatatc tctttgcatt
     1201 tcaagttaaa cccgaaagca gtttcttgca tttttttcat gttccgttaa catgaaatag
     1261 agaaagaaaa aataaagggg gagcaataat atggtcgtca aaactgagac tttagaagaa
     1321 aaggcaagag aactattgaa ggaacgtggc gtaactgtag aagatatcgc acaacttgtc
     1381 atgtttttac aaaaagacta tatttctgat ttgactttgg cggattgtac tgaaagtgtc
     1441 aacagcgtgc taacaaagcg tgaagtacat aacgccatta tcactggaat ccaattagat
     1501 atattagcag aaaagaaaaa attgatgagt ccgttgcaac atattatcga agatgacgaa
     1561 ggcctttacg gtatcgatga gatcctagcc ttgtcaatcg tgaatgttta cggtacgatc
     1621 ggctttacga attatggtta tatcgacaag gtgaaacctg gtattttaaa agaactaaac
     1681 agccacgatg gcgttcatgt ccataccttc ttagatgata tcgtcggagc gatcgccgct
     1741 gctgctgcca gtcgattagc acacaaagaa ccagaaaaaa gaagtcgcct tactcaataa
     1801 ttcaacaaaa aaagaaagaa aacgtctatt tagacgagtt ctttcttttt tgtttgtttc
     1861 aagctatttt ttcctaagaa aagaaaaaca gcaaaaaata attctaataa tacaaaaaat
     1921 aagataaagc tatgcatgaa aaattcttct ggaagtacat tgatgattgg atcagtaacg
     1981 ggatcgaata accagtcatc gttgttgaaa aacgtttcat gaaaaaggat aaaaaagcga
     2041 tcaaatccta tcatcatgaa aaaaccgaaa acaactggca gtaacatgcc gatttgaaac
     2101 ggacgaatca accgccaaag ccgatcattc ttttgcaaat atttgataaa caaaatactc
     2161 gggatcaaag tgatgaaaaa cacagcataa tccactaaaa acaaaatttt cacttcataa
     2221 aagtgatggg ctccgctagc agaaactggg aaatctggca agctgaggat ggttttgaag
     2281 ggattattga gataattcat taggtgatca aaatttttca gtaacgtctc ttgatctaaa
     2341 gaagtataat ccaaaatttg caaatgtccg atatcaaata cgtatagtgg tcgaaagttg
     2401 atcgtcacac caactgccag actgatcagt gtcagaaata ggctgaacaa tccagcatat
     2461 tccatccact gccatctcga atttttcata ttagaagctc cattcatcca gtgattgttt
     2521 cacataggta gctgggatag gcaacccagg tacatcttct tcttttgtaa aacctgataa
     2581 aacgagcaaa gtatctatat tattgcgtat cccagcttgg atatctgttt catagttatc
     2641 tccaaccatg attacttctt ctttttgcag ccctaatact tcaacggctt tatccataat
     2701 gatggcttct ggttttccga tgtagatcgg cggcgtttgg gttgctgtct ctacaaaaga
     2761 gatgactgac cctgctccag gaagaaggcc gcgttcagta ggtatattct tatctgggtt
     2821 cgttccaatg aaagtagcac ctttttgaat agcaagtgtt gctttaacta ctttttcata
     2881 ggtcagataa ttatctaagc ctaccactac atagtcagga gcctcttctt cccatgtaaa
     2941 tccggcagct aaaatcaagt caatcaatcc tgcttctccg ataacataga cttttttccc
     3001 ttttccatct gctttcataa aatcgattgt tgccaaagta gctgtgtaga ctgtctctgg
     3061 agctacatga atgtcaaact catctgctag acgattagct actgtttctg gtgacttagt
     3121 cgtgttattg gtaacaaaca agaaaggtaa ttttcttttt tgcaattctt ccacaaatcg
     3181 tttaccggcc ggtatgggtt gtgtcccacg ataaatcgtt ccgtctaaat caattaaata
     3241 tcctttatag tccattcatc cactactctt ccctttttct gatcgtaaat gctgtttttg
     3301 ttttttctac ttgtttaggt tgttcttgtt tttttacttt ttcagaggtt tttgctgact
     3361 ctgtttttcg atttttaata accggttttt tgcgattaga tgcgactggt ccttttttct
     3421 catcgatata cgcacgatta tggttttttt tcttttttcg gcgctgagtt gtcttttctt
     3481 ttttaccgcc gattctctcg atgacaaaat acgcacaacc aaaattgcaa tattcgtaaa
     3541 gatagtcttc caaggaatcg attcgctgat ctggatgcgc ccgtcgatta tccgcttgga
     3601 aaaatccttt taggcgtagt tgttcataac cccaatctcc tacgatataa tcatatcggg
     3661 ctagtacatc actgtaacgt tctccaagct tttctgcatt aaatccttca cgataatcgg
     3721 acacaagccg ataatgtctt tcacctatca aaaaattact gtcatctaac agtgtcacta
     3781 gctcgccttt gactttttct gctttaggct cctcaacgat ttcttccaaa gcagctgtca
     3841 gttcttcggt gactgttgtt tcctgttcag ctgatgattt tttcttagca atttctgttt
     3901 tatgctcaac gttctgctgt tcattttgtt ttgcagacat gcgttcacca cctatctttt
     3961 ttattatacc ttgtttttat ttgattgggt agtgagcttt caagtaatct cctaccacac
     4021 ttcggataaa ttcagggaac aaaaattcat tttttccagc aatctcaatc gttggaaaaa
     4081 atggaacaaa catcaaatga tctacggtag taaaagtata atttttattc agatcgattg
     4141 gcttgttctt ccaaaagact tgatggttaa tagaatcata tgtgattcca ttatagacca
     4201 tctgcccaaa aatcttaccg cgaaatccca ttcccttcat aggatagtta cgtaaaaagt
     4261 tacgattttt ttctatttct aaaactaagc ggatcagatc atttccaccc aatgtgacat
     4321 ttaacaagtg catcggatgc ggcaacgcag tgtgaagctg atcctgattg acccgccctt
     4381 ttggaatttc agttaaaaac agaccactgt ttaatattgc tgcttccgtc tttcctcgtt
     4441 ctttcaccgc ctcaaaggct acttggataa acgaatgctc attgaagata tctagactca
     4501 gagaaaatgg caaatctgct acttcttttt cttttaataa ttcatgccca cgttctagat
     4561 aactatttat ttcttgttca tcttctgtaa aagctgtcat tgtttcggta gggatggctt
     4621 tggctgaatg cttaaggatt tttttctctt catcgatggt taaatggact tcaccgacat
     4681 actgcccgta ctttccagcg gctgctattt ggacatcatt gattacgtga ccttctctga
     4741 ataaatgatg ggtgtgcgat cctaaaataa catcaattgc tggtatttct tttgcaattt
     4801 ggaaatcatc ctgaatcccg agatgactca tcaaaactaa aacatccact tgtgaacgta
     4861 aatcttcgac taattctggc aaaatatcat aaggattccg tatgtcccag ccattcggat
     4921 tataagtaag cggaaatgga gcagtaaatg caattagtcc gattttcgtc tggtgtttcg
     4981 ttgtaatgat tttatatggt ttcgtccatg ccggtggctg gagcgtattt ttatcgaata
     5041 agttatccag gatgatatca aactcagcat gatcgtataa atgattcaac tcttctttgg
     5101 aatttcctac gccttcattg tttcctatcg tcgctgcatc ataaccaact tcattcatca
     5161 gcaagatatt ttcctgtcca ttcgttgctt ctgacaatgg atgccaacga tccacaaaat
     5221 cacctagatc tactgcaatc gttgtcgttt tttctttctt ttcgttttct cgtttacgtt
     5281 gatccaaaaa ccttctgatc cttggccaat tttctaaatg agaatgcaga tcattcgtat
     5341 gtaaaatcac tattttttcc atgtaccctc tcgcctctct ttatctttat cataccatag
     5401 gcagcttttt cttccattga aacaagctct atttcagagt ttttcctgcc ttgaagcaaa
     5461 ttgtataagt acaatattcg ataaatcagt ctggctaaaa aatcaggaac attcattttt
     5521 acagtactat aaaaataaaa aaaacagacc cgaaagtctg ttgtcgtttc attataatca
     5581 gacacatggc ggaacctgct tagtgctgct tccttccaga cctgacacgc ttcacaagcc
     5641 caccattgtc ctagcctttc ggcaaagaat agtatacctt atttttcatt cgttgaccag
     5701 tcttttttta atttcttttt tcgctggcgg agctttcgga aaaagtcagt caaaatcatc
     5761 ccacattcgt cttctaaaat gccaccttct acatatgctt ggtggttaaa ccgctcgtcc
     5821 tccaaaagat tcatcaaggt accggctgtt ccgccttttg gatcatacgc gccgaaataa
     5881 acttctgcta cacgagacaa aatcatcgcg ccgctgcaca taggacaagg ctctaaggta
     5941 acaaaaagtg cagcatcttc caagcgccag cttcccactt tttcacaggc ttccttgatt
     6001 gcaagcattt ctgcatgtgt agtagcatct tgcgtggttt ctctaaggtt atatcctctt
     6061 ccaatcactt ttccatccaa tacaaccact gctccaatcg gaacttcatg aagcatttct
     6121 gctttctttg cttctgccaa tgcaaggcgc atccattttt ctttttctac tgcatcaaga
     6181 tttgtattta ccaattaccc ttccctgcct ttgttttttc ttgttttact ttagcatatg
     6241 ataaaaaggc tgtaactaac ttttttttct atggtaaaat aaatacaaga ggtgaacaag
     6301 atgaaaatgg ataaattact tgctctttac ccacaagcac agaaaaaaaa ctctccagca
     6361 tcagaagcgg atatcctttc ttttgcaatt gatgatgctt ttatctggat caaacaagat
     6421 tcattatctc aacaagaaac cagccttttg aaagcgttat tccctgtaat caacgatcaa
     6481 aaaaaacacc catggtatca ctatcttttt aaagatgctt cttgtgttca agaaaaaagc
     6541 tttcgtttga tccagcttca cgtcgaatca aaaggagaat tcttaaaaaa agaatggcaa
     6601 gatactattt tggaattatt tcgctattct gctgatttct ttttttatac agacacagat
     6661 gcgctcttag tcgaacaaat aaaccgtgat tatcttgatg ctactgaaat caacggtatt
     6721 tttctatctt tagatgctga ttttgatacg acaacgtctg tattcgttgg ttctttccat
     6781 tcacctggca ccgacctcgt ccctttgttt gctgaagaaa gaaaaatttt tttaacagag
     6841 caaaacaatc tttatactca cagccgtact ttttctatgc aagatgtggc tttacattac
     6901 tatacaaaag atactgtaaa agatagtaag ttgatcaaaa gttacataga actgatcaaa
     6961 caaaatgaaa tggaagtgat cattcaagaa ttatggaatg aattaggcaa tgtcagctct
     7021 gctgcaaaaa cactgtttct tcatcgtaat actctaaaat atcgaatcga aaaatttcaa
     7081 gaacagtctg gtttcaatct aaaaaaagcc aacgatttac tgttttgcta tcttttattg
     7141 cttcatgaac ataattaaag atagattaaa ggggttgtga caataatttt gtcacaaccc
     7201 cttatttcga atgaatagtg ttcaaaagct agtttctctt acatcatttt ttcaacaaaa
     7261 tgcattagcc ggtcttttac atcttcggtt gaatctttga ttgaatcacc ataaacagag
     7321 attttctttt ttcctgattt gatttttgct aacattccca tcaaagaatc caagtcacta
     7381 tctgaaagac catctacgat ttctagtaat ttttcatttc catcaaattt gtcatagacg
     7441 aattttttta ctttcgtccg agtagtcata tgatggatct tttccattac ttttcccgat
     7501 acaacgactg ctgtcgctac actggcagcg gctgctacgc ttaatccgat tgttactttt
     7561 gttgatgttt tcataagtat cagctccttt ttatctatct ccatcatagc atagacaaaa
     7621 aaataaacga actaatccac tcagtgaaat ctcaatcctt tagggaaaaa gttctttact
     7681 gttcctttta cttgtttccc aaatccgaca ggtatcttat ctaatactag caaaacccat
     7741 ccttgattcc catcacgttg aaatgtttct cctgctgtgt aagattgcca ttccttttga
     7801 gtgatcggca agcaaggtat attctctatc ttttttgtag caagcgctaa tgcatagcta
     7861 ggttcaaatc gattcttttt gaaatctcct aaatgcaaac ctgtacgtac gacttttaaa
     7921 ccatcgagag aaggggctaa ctcaggaact tcccaaagat ggtcgttgaa cactaaaagt
     7981 cttccagtag cttcataatg aaaatcattt gaaaactccg tccagagttt ttcctgctct
     8041 tttgtcattt gcactttact tttttttcgt gtcttctttt ctttatgcat ctgattttgt
     8101 ccatgaaaag tcagctttgc aacaaaatgt ccttcaccct gatctttatg aggccatata
     8161 cgtattgttt tttccaaacc agctacgctt ccccattcag aacgtcctga actaacggat
     8221 tgagtcaaag gaatctcctc aatcgttact ggataatttt ctacaagcca gctaatgatc
     8281 tcttcatttt cttctggtgc aaacgtacaa gtcgaataaa tcagttgtcc tttatttttt
     8341 agcattttta ttgctgaaga caaaatctct tgctgacgtt tttgacagta tagcggagat
     8401 tcttctgtcc actcttttat tgcatttgga tcttttcgga acattccttc tcctgaacaa
     8461 ggagcatcaa ccacgatccg atcaaaaaag cccgaaaaat gagggacaag ttctgcgggt
     8521 gcatgatttg tcactattgc gttcgaaact ccccatcgtt ctatattttc tgataaaatc
     8581 ttcgcacgtt tcggaaagat ctcattcgtc actaataacc ctttcccctt catttgagca
     8641 gctaattggg ttgatttacc accgggagcc gcacacaaat ccaaaacttt ttcaccgggt
     8701 tttgctgcag cagctgttcc aacgatcatt gcacttggtt cttggctata ttcataccca
     8761 gcttgatgga ggaaagactt tccattgaca gtgccaagaa atccctcatt gctgtaggga
     8821 gcgggttgta attcttcgct atggtaagtt tggaccatgt ccaatcctgc cggttttagt
     8881 ggattccacc gaaatccttt tttcacgctt ccttgttcaa gcgcagaaaa aaaatcggat
     8941 gcttcttcgc ctaaaagaag acgatatttt ttgataaatt gctgtggtaa cgttgtcgct
     9001 tcctccttca ttccaatgtt tcgttagaca ttttacaatc ttttttacga aaaataaagg
     9061 tggctgatct actttttcat gttgctgcct aaggtgactg ttcctttaag atattttcca
     9121 agtattcatc cgtcacctcg tttttatccc aaacagcgtt catcatttca agaaaatctt
     9181 gagaagaatc atctgagcgc aaacttccca atgcattaag ccataccaat tcaagcgact
     9241 tttcttcttt gatcttcgtg tgacctttga catatgagaa aaatggccgc ttaaatagcg
     9301 aatccatgtc taaatcagat tttgcaatga tttcacgaat actttcatgg atagaaaaaa
     9361 aatctttcaa taagtagaca atgcttaaaa catcatcgga actgcattca gctaagtagt
     9421 tttcaaaata aacagtgatt tcttctaata tcttctcttt taacccgctg attgatttga
     9481 aatgtgtata aatcgggtat gtagaacaac cgcagaactc tgctaactct cttgcagtaa
     9541 ttgacttcat tccatattct tccacgtata ccat
//
LOCUS       DACDPS010000088         9398 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.088, whole genome shotgun sequence.
ACCESSION   DACDPS010000088 DACDPS010000000
VERSION     DACDPS010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.088"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            165..359
                     /locus_tag="IWJ07_002291"
     CDS             165..359
                     /locus_tag="IWJ07_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333831.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            628..927
                     /locus_tag="IWJ07_002292"
     CDS             628..927
                     /locus_tag="IWJ07_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333832.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1024..1458)
                     /locus_tag="IWJ07_002293"
     CDS             complement(1024..1458)
                     /locus_tag="IWJ07_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9333833.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1690..2400)
                     /locus_tag="IWJ07_002294"
     CDS             complement(1690..2400)
                     /locus_tag="IWJ07_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP9333834.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2390..3250)
                     /locus_tag="IWJ07_002295"
     CDS             complement(2390..3250)
                     /locus_tag="IWJ07_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAP9333835.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3255..3893)
                     /locus_tag="IWJ07_002296"
     CDS             complement(3255..3893)
                     /locus_tag="IWJ07_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAP9333836.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3908..4207)
                     /locus_tag="IWJ07_002297"
     CDS             complement(3908..4207)
                     /locus_tag="IWJ07_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9333837.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4250..5734)
                     /locus_tag="IWJ07_002298"
     CDS             complement(4250..5734)
                     /locus_tag="IWJ07_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP9333838.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5754..6215)
                     /locus_tag="IWJ07_002299"
     CDS             complement(5754..6215)
                     /locus_tag="IWJ07_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9333839.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6233..7300)
                     /gene="ulaG"
                     /locus_tag="IWJ07_002300"
     CDS             complement(6233..7300)
                     /gene="ulaG"
                     /locus_tag="IWJ07_002300"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAP9333840.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7518..8288
                     /locus_tag="IWJ07_002301"
     CDS             7518..8288
                     /locus_tag="IWJ07_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9333841.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8561..9388)
                     /locus_tag="IWJ07_002302"
     CDS             complement(8561..9388)
                     /locus_tag="IWJ07_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9333842.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2917 a         1924 c         1481 g         3076 t
ORIGIN      
        1 gaacaatagc cttttattat atatattatt caaattagtt atatcgagtg aaaaaaactt
       61 tcaaattgat tctttttatt tatcataaag atatagatac ataatcattt tcacttacat
      121 atatttgaat attaactaaa aacagcaaga aagaagagga tcaaatgtat tatgtagaag
      181 taaaaacaaa aggcgtcaaa aacaaacaat atgtgaaagg tatcagtaac gaatatcctt
      241 tacttggttc atggaaagaa gctgctcctt tttccaaacc atgtgccata aaaataaaga
      301 acgagctaga aaaagaactg acttgtggta aagctgttgt agatatcatc gaaaagtaaa
      361 tccaatgtat agaaaggaag atcacacatg aaaagcgacg tacaaattgc taagattata
      421 gaattagaag aagaagacgc agtgttgaca attgaaaaaa aataaatgtt tgtataccat
      481 atgattattt caatatgtat cctggaattg gagatctcgt agagctatat caagatgaat
      541 caaatatact tgtcattcat catgaaatat agagacttat aaaattgttt atttttataa
      601 tgtatcagta tagaaaggaa ataaaacatg gattatactg ttgaatttga cagtgaacta
      661 cttaagcaat ttccacaaga agaacatttc atgtataaag aagcaatttc ccacaaagtg
      721 acagagtatt tggagcataa tttttttcgt ataaagccag tgcgtactcg ttcggcaaaa
      781 ggaatatacg agatgaaagt caaaattaat aaaaaaaatt atcgcatagc atttgcttta
      841 aaaaaaaatc atgtgttcat tttttatatt agttcgaatt tgcaaaaaat tgtatttgat
      901 aaagaggtga gtaaaaaaat aaaatagctg agcattggag gatagattga gtgatactaa
      961 gtctatcctc ttgtttttct aaagaactaa gctagaattt tttcttttgt acgttattat
     1021 atgttatttg tcattaccaa aaagcacctt ttttcttcta gtatctatga cataagaaat
     1081 accacccatt tcctttactt gaacaaatcc tactgattca taaaattttt ttgttttctc
     1141 agtatcatct gtcaataatt cgatttggac tggtccataa ctagtgatct tctccaaagc
     1201 ttcattcaaa agcactttgc ctattccttg gcgctgatac ctcggatgaa tgaggatatc
     1261 ttgaataaat gcgatcgtcg caaaatcagt gatccaacga acgattccga taatcttccc
     1321 ttccccatta cgctttatca gagattcaga ttgttcaaat gctctagcta accttgcagt
     1381 gtcttttgtg taattcgtcc aaccaacgct cttatataat tcagaaatct catcgatcat
     1441 aagggaatca atattcattt ttttcttcct ttcttataca attctccctc taataataca
     1501 gttctttgac tatttaatct atcacatata actctcctgt aaagattctc tcgattcctt
     1561 aaaattcctc tttcctttta gaatgcatac tttttgataa ataaaaaggt tgtgataaat
     1621 tttcttcaca acctctgttt ctctctatca agtgttatct tttaattttc tacccttacc
     1681 ctagcatctc tattgcccgt agtatgcata ctttccatgt ttgcgaaaat aatgtttatc
     1741 tagtaaatat tgcgggattt cacatatatt ggggttgatc agctcgtttt ctttggccat
     1801 cagacaaatt tcatctaaga tcaaactgtt ttctactgct ttcataggtg aatcccccca
     1861 tgtgaacggt ccatgtccat agacaagtac tccagggact tcgtttggat ctaattttct
     1921 ttccttaaac gtctcgacaa tcacattccc tgtatgaact tcgtaagctt ctcttacttc
     1981 ttctttcgtt aattggcgtg tacaaggaac cttaccataa aaagcatcag catgagtcgt
     2041 gccataagct ggcaaatcgc gtcctgcttg cgcccacatg acggagtgcg tggaatgagt
     2101 atgagtaata gcagttatat cttcaaaagt ttgatacaaa acaacatgtg tggggagatc
     2161 tgaagatggt tttaagctat cttcttctat tatatttccc gacaaatctg ttaccaccat
     2221 ttgatctgct tgcatgcatt catatctgac accgcttggc ttaatgacga tgactcctag
     2281 ctcacggtcc acttcactca cattgcccca agtcaatttg actaatccgt atcgcggcaa
     2341 tgccagattt gcttcaagga cacgttgctt cattgctgca attttttctt cacaagacat
     2401 catagccagc ctccttcaat ttagaactta agtagtcttt tgcctgctgg atttctcttc
     2461 gaaagtcttg gcttttttca ctccacattt caatcagaaa tgttccatca taatctagac
     2521 gtttcaaatg tcttaataat ccaagaaaat caacacaacc gctaccaaaa ggaacatcac
     2581 gaaactggcc tgaatattct tttgttacag ggtaagtatc ttttaagtgg atagcagtga
     2641 tataatcgat ccctttctca agttctcgag ccggattatt ttccggccat gcggacaaat
     2701 tgcctaggtc tggatatact tgtagaaaag gtgaatgaat ctgttctttg atttctaaaa
     2761 attttgaaat ggagttcata aatggatcat ccatgatttc cacggaaaga atgatgccat
     2821 atttagaagc ttcctttacg ctttctttta ttccttcgat aaagtcttct ctggaattca
     2881 ttgatttctc ttcataataa acatcgtatc cagctatttg gattactttg acgcttagat
     2941 tatgcgccaa atggattgct tttttcatta gttgtttcgc tgcttttttc ttttctggat
     3001 cagcagaacc aaacggaaat cgccgatgcg cacttaaaca aatcgaatgg atacgtacac
     3061 cggagctgaa catctcttct ctcagctcag ctatcttttc ttcagaccaa tctaatcgtg
     3121 ctaaccgctc atccgtctca tcaatcgaca tctcgataaa atcgaagtcc atttctttta
     3181 caagggaaaa acgttctttc catgaaatat ctttgggtaa cgctttttca taaatcccaa
     3241 tttgagccat ttccttaccc ccagatacga atgatctcat ctttgaagtt ttgtgcggcc
     3301 ttcatcggat catttgctgc tgtaatacca cgaccagtga taaaggtata cacatcgatc
     3361 ccttcaaata agtgtaatgt gtgtggattt aatccaccag ttacagaaac attaaagcct
     3421 agttcaatca gttttttgat cttcgataaa tctttttcac cccaagtttc accagataac
     3481 agcgcatcac ggctttgatg gtagattacc tgccggatac cgatgttatg ccaatccatg
     3541 gcttgttcaa aagtccaatt gccgtagagt tctacttgca attcacctat ttctttttgt
     3601 gctgcctcca tagttggaat cgttgcacaa caaatgactg tcatccagtc tgctcctgct
     3661 tttgcaacat tactcgccac tgttcctcca gcgtctgcac atttcgtatc agcgactagt
     3721 tttttatctg ggaacatact gcgaatacag cgaatcgctt tttgtccttc ctgtaagcaa
     3781 agaattgtgc ccacttcaat aatatctact acatcaccaa ctttcataac atctcctaat
     3841 gcatgttcta agtcattgtg atctaacgca atttgcaaat tcggcctgct catcttagtt
     3901 ccttccttta cttgtttttt tggtaccact ttgtttcagc aaatttttca ctgatttctt
     3961 tgactgacat cacatttttg ataccgataa ctgtaactcc ttttttctct gcctgagtaa
     4021 acatattcaa aaaattgact ggcgtaaaaa ctacatcgta ttggccagct gaactttttc
     4081 cctctgaaat ggcacaatga tggattttgg taattggtac gtttagttct ttcattgctt
     4141 tttccacact tctcatcatc atcaagcttg ttcctgatcc atttgcacac gatactaaaa
     4201 ttctcatctt aatccctcca ttatgagtgt tgtctaatca gattttcttt tatactctct
     4261 gcttttctgc atatgtgtca tagtcttcta caatcaaaaa ataaccatca ggatttttcc
     4321 gatattgtaa ctgcggaata gccaataaac caatgacaac tacggcaatt ccagcataac
     4381 ctaggacttt catgattact gtaaacaatg gccaagctgt tgcccagtcg aacatcccga
     4441 tataaccacc gtatgttgac atcccaatcc atgtagcgaa taatgctgaa ccgcctactt
     4501 gtatgattcc tgaaagaaac gggaacaggc aagcggcttt gaatccacct cggttattgg
     4561 cataaactgc tataacggca ttatcaaaga acaacggaat gaaaccggct atgacgatcg
     4621 ttggtgattt caataaaatc agtaaaccaa tcatcaaaaa ctgtcccaaa gcaccaaaca
     4681 agaacccaat cgttaccgca tttggcgaac caaagccaaa agtagcagct acgtcaattc
     4741 ctggaacagc tcctggtaaa atcgtgtttg agattccttg gaaagattga gttagttcat
     4801 cgacgaatgt ccgaacacct aattgcaaga tcgccagata taccgcaaaa tttaaagaag
     4861 tcgtcataat gtagaataag aaactctgcc cttcttgcat gaattgcgct tgaatcaaat
     4921 aatctttgcc tagtacaatc agaatgatcc caaagaaaaa tagcatgagt aacgaagtag
     4981 caaccatatt ctcattgaaa atcgacaaga atcctggtaa tttgatatct tcaatttttt
     5041 tgtcttgctt gattgatcta tttttctttt catcccgctt tttcatccac tcagctaatt
     5101 ttgcaaatat aaagattccg aacatttgct gatgtgccac tgcaaatccg gcaccttctg
     5161 tcaattcctg cgtgattcct actgttaagt tagaagctac tgtccaataa caacctaaaa
     5221 tcagtcccat aaaaatcaat gcttgtatcc tacctagatc aggaaagcaa aacagtaaaa
     5281 tccaaaaagc ggtagctgct tgttgaacct gaacgtttcc ggtagtgaaa acggaacgca
     5341 gtttcgtata tttctgaaaa cggactaata agatatttaa aacaaatgca atcagtaata
     5401 aaatcatcac atctccaaag gtacggccaa atgtctccat taatccagca tccacagcat
     5461 tttgtccaaa ataaggatct gtaaccatgg catccaaatt gaaccgttcc tttagtccaa
     5521 ccaagattgg ccgaaaatta ttgaccaaac caccagagcc aacagtcaaa ataaagtaac
     5581 cgaccgttgc ctttaagaat ccagcgatgc attcatgaag cgggcgtttc agcaaaatat
     5641 agcctaataa cacgataaaa ccaatcaggt atgctggctg cgtcaagata tttgttgcaa
     5701 aatacgtcca aatgcttaac aaaaattccc ccatcttttt tctcctctat caattaatta
     5761 tattctgcca tcacaacttg gtaatcatcg atggagttcg tagagatgag acgctcaacc
     5821 aatccatctg tcattaataa ttcggataat ttttggatat tttctagatg ttcttctgga
     5881 tcttttgctg cgagtgtaaa aaacaaacta gccgttttct cctcattctc cccttcactt
     5941 tcaaaacgga ttggctgttt caacttggta aatgaaatag ctgttccaaa aacaccatca
     6001 cttttttctg aagaatgcgg cattgctact tgaggtgcga tcacaatata aggaccgtat
     6061 ttttctacac aatggatgat ctcctccaca tacgtttcat caataatttc cttatcgatc
     6121 aatacaagac agctttcggc gatagcttca cgccaatccg tgatttcttt atctgggtaa
     6181 tgaatcaatt gatggtcaaa aaagtatttc agcaagaaac tccccttctt tcttataaaa
     6241 acgacggaaa cggtgtatca ttttcaatcg agaagacatc ttcaaatcct cgataaaaat
     6301 taaactcaag atcatctttg tttgtcggat agatgaattt cccgcctact tgccagatat
     6361 aaggtttaaa tccatacttc aggcgatcct ttttgaattt ccaaatttca gtgatctctt
     6421 ttggatcagc cataaaatta gaccaaatat cgtagtggac aggaatcact acttttgcat
     6481 tcaatgactc cgccatgcgc agcatatcaa cagaagtaac tttgtctgtt atccctcgtg
     6541 ggttttcacc ataggcccct aaacagacat cgattttgtg ttcattacca tgtttggcaa
     6601 ataaattgga gtaatgtgaa tctcctgcat gataaagatt ccctcctgat gtttcgaata
     6661 aatagtttac tgcaatttga tccatctcct gaggcatttt tccttttaat acctcttctg
     6721 gatcttcaca agtgaccaac gctgtccgat caaacgcttc taaagctact acattgatgt
     6781 ctttgatttt gactgcatca cctggcttta ctgtaatcgt tttttcttca ggaattcccc
     6841 atttttgcca cgtgcgaacc acttcttttg gcccaatgaa tgttgcttca ggacaatttt
     6901 gatgaactgc tgcggctgta tggatatcta gatgatcgga atggatatgt gtaacgatta
     6961 aagcgtctac attttttatc gcaaaaggat cgattacgaa aggctgagta cgtaaattag
     7021 gctgcatatt ttgaacgcca ctcatacgca tcatctgatg gccttttttc atcatgccat
     7081 tcccatgact acgttttccc gtcccacacc atagatcaca taaaatattc gttccttcat
     7141 gacttttcag ccaaattccc gtacagccca gccaccacat agcaaaagtt ccaggatcca
     7201 ctacttctgt ttcgatttct tcattcaagt atgtgcccca ctcaggaaat gtttctacta
     7261 accacttttc ttttgttact tcattgacag ttttactcat ttgcttgctc cccccatatc
     7321 gttgttccaa cttacaagta aatattaatg tgtatcagca tccatgaaaa cccttttata
     7381 tgaccgtttg ttttttatat gattttcttt cagtaatcaa tgattgtttc agtataataa
     7441 agacagatat gaatgtttaa atgaacaact agtcaaataa atccatggat caagatgtcc
     7501 ctcaggaggc aaaagtcatg aaaaactcac ttgtcaatat tgaaaaacgt caaaaagaaa
     7561 ttttggaact gcttcagcag actgaacaac tatcaacttt cgaattagct gaatcccttg
     7621 atgtatctat tagtacgata cgaagagatt taaatttatt agaagaaaaa aacgagatca
     7681 tacgcaatta cggttattgt tcttataatt acaaaaacca gactgatttt gatcaatctg
     7741 gtcctgaaag gattaaacaa gcgattgcca gagaagctag taaatacatt aacgattatg
     7801 atacgctgtt tatcaattct agctcgactg cgttgaaaac actggatttt cttcaatcta
     7861 accatttgac agtcattaca aacaacgtga aaatcaatta ctctccgcac aaagataatt
     7921 gtagttatat tttaactggt ggagagatgc gctttccaaa agaagcacta gtgggcgaca
     7981 tcgcattaaa tacgatttct tcagtcaatg cagacatttg tatcattggc tgtagcggag
     8041 tcgatgtagt acatggggtc acaacaaaga tattaaatga atcaacgatc aacgaaatga
     8101 tggtcaacaa gacaatcaaa tgtaagattt tagtcgctga tcatcgaaaa atcggactga
     8161 catcaaagtt caagattgct gatatttcag tctttgatta tttgatcact gataaatttt
     8221 gttctgaaaa agtattagag gagatcagaa atacggggat aaaaaccatt caagtagatt
     8281 cattttaaga accaggacgt aaatatgtaa ataggattat caaaaaaatg aataacaaat
     8341 ttatcgataa atgaaaaaca cgctacgctc aaattgataa aggaatccca agatgtatcc
     8401 atccaatcga taccatcata atctattgct tcttttataa ccgttatatg ttgcctaaat
     8461 aggaatcaaa caatatcgaa aaaaggaggt atgacaaaag tcacacctcc ttttttcgaa
     8521 taaacggtat tcaaatcaga acactgattt cacaaccact ttatttcagg tcgttttcaa
     8581 tcacctttac cgcgtaatca tttccgttga aacgcgctat tttttttgcg tccatatatc
     8641 tttctaaata atcatcttct cctaattgct tatggcaaga agcaatttca taaagcaagt
     8701 attccaatgc ataagtcgat ttatgtttct tattatactc aatacccttc tcacataatt
     8761 ggattgcttt atcataatcc tcgattttac ggtaataggt ggcgatatca taatatacaa
     8821 tgcagaccat cagattatca aaatcagtct cactcaatat tttaagtgta cgctccacat
     8881 aaactttcgc aaaatctaat tctccttttg acaagtagat cattgccacg tttgcagctg
     8941 cttgtaagtg atggatatct gtttcggtcg tttgttccaa cacttgatta aaataaaata
     9001 atgctgcatc tggttttttc gcataaagat gatcaagcag tccttgataa aataaaattt
     9061 gatttaaaag atcagaatta cttacttttt cctgatcgat cgtttgtaaa attttataag
     9121 attcttggta ttttgataca gaattcaatg attgaatctc atttattttt ttcctgattt
     9181 tgtcttcttc tgtttctact aatatatcgg agatctcgac attcagcttt ttgcaaagcg
     9241 cacttaagat attgatgcct ggcgacatat tcttgttttc gatattgctg acagttgctt
     9301 gttcacaaat gccttctgct aattgtactt gtgttaaccc ctttttcttt cggtattctt
     9361 ttagtaatgc tccatctact ttaatcaaaa caatttcc
//
LOCUS       DACDPS010000089         9379 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.089, whole genome shotgun sequence.
ACCESSION   DACDPS010000089 DACDPS010000000
VERSION     DACDPS010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9379
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.089"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(120..788)
                     /locus_tag="IWJ07_002303"
     CDS             complement(120..788)
                     /locus_tag="IWJ07_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9333843.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            922..1209
                     /locus_tag="IWJ07_002304"
     CDS             922..1209
                     /locus_tag="IWJ07_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333844.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1625..3163
                     /locus_tag="IWJ07_002305"
     CDS             1625..3163
                     /locus_tag="IWJ07_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAP9333845.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3160..4125
                     /locus_tag="IWJ07_002306"
     CDS             3160..4125
                     /locus_tag="IWJ07_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9333846.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4112..5434
                     /locus_tag="IWJ07_002307"
     CDS             4112..5434
                     /locus_tag="IWJ07_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9333847.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5497..5601
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5675..6418
                     /locus_tag="IWJ07_002308"
     CDS             5675..6418
                     /locus_tag="IWJ07_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9333848.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6433..7425
                     /locus_tag="IWJ07_002309"
     CDS             6433..7425
                     /locus_tag="IWJ07_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9333849.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7437..8174
                     /locus_tag="IWJ07_002310"
     CDS             7437..8174
                     /locus_tag="IWJ07_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9333850.1"
                     /translation="MSIQISHLNVVIENLKVLEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8164..8886
                     /locus_tag="IWJ07_002311"
     CDS             8164..8886
                     /locus_tag="IWJ07_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP9333851.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8970..>9379)
                     /locus_tag="IWJ07_002312"
     CDS             complement(8970..>9379)
                     /locus_tag="IWJ07_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAP9333852.1"
                     /translation="QLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEKQAAP
                     VAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYLNGDY
                     SAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3075 a         1609 c         1974 g         2721 t
ORIGIN      
        1 ttcgcagatc gtttgcaaaa atagtttatt tttttataaa gaagatttta ctgaataata
       61 tcattttagt ttacataatg atatttctgt aaattaaata aagtagtgaa aaataagaat
      121 cagtgctcta actgttcaag aggatcaagt ggttcaaatg tttctgtagt ctgttttgct
      181 tccaattggg gcaaaacaga tccaatttcc agatacttca ctttattcaa tgtataagat
      241 agaatagaac agctaagtaa tgtatacaga aaatgtgtga gatccaaata aagcaaggag
      301 acaactaaaa taccaaaagt aatcaagaaa agacttttgc caatcttaat atgaaaatat
      361 ttttcaatgg cttgataaat gatatctgtt ccgcctgtgc ttccgccgaa tcgaaagacg
      421 agaccgcaac caatgccaga aagaatacct gtcatcaaac tattgatcag tagactatct
      481 acagcaaaat gtatatgaag gctctcccat aaatgtaaaa agaaaactag cgcaaatgtt
      541 ccgacgatcg tccggagaaa agcgtgtttt ccaaaaagaa acaatgagat aagcaataaa
      601 ggtaaattaa aaagcagtga agtgacagaa ggagcgaatc caaaaagatt atgcaaaagc
      661 aaagcaatac cagtaacccc accatctgcc agcttagatg gaatattgat tcctgtcaca
      721 gctaatccgt agattcctgc gcctaaaatg attccgcaca attctgtaaa taaatttaat
      781 tttttcattt ctagcctctt ttcttttgaa aattttcagc aacttcttta ttatactgac
      841 ttatactctt acttcaagag gaaaatgaaa aaaaattatt tttggctgtt tctttttatt
      901 tatcaaataa ggagtggaaa aatgcgagca gcagacttat tgacaattct tacaaaatca
      961 cccaaaaaaa cagagtatca aggagttttt ttggctcaag aacaaaaact ggtgcctgtg
     1021 acaatggtcc agataaataa agcaaatcaa tttatctttt tatttgaaca cggcaaagca
     1081 ccacttgcta tgaaagacat ctttcttttt ttgatgaaga accgtgagaa agctgtttat
     1141 tatcagtcaa aagaagaact cattcctctt tatggagtga aagagataga aaataaagtg
     1201 gtgatataag taaaaataag aaaacaagac gtttatgatg tcaaaacaac cattttttac
     1261 atttttggca aaaaagtgtc atgatacagt ataatattcg ttgaacaggc caataaaggt
     1321 gttctccccc acgagatttc tactttattg gctgtttctt tttattaaaa taaaagttgt
     1381 gtgtgtaaaa aagatatagc tgattccaag tgacaatttc gtatagattt ttatggtatt
     1441 cgtagtgaaa aaacgagaac caaaaagttg atccgacttt gttgcttgga ttcttttttt
     1501 gtcagcatat ttagaggtat ttttttcttt tttttgttcg tgctgtaagg ttaaggcaag
     1561 acaagttagt tgggaaaaag gaaagaaatg tttatactta aagaataaca gaaaggaagg
     1621 tcgcatgaat aattttatac aaacattaag tgaaagaaaa ggcgagttgc tggaagctgc
     1681 atttcaacat ctgtccattt ctctcatttc tttgctgatt gcagcactga ttgctattcc
     1741 tttagcgatt tgggcagcca atcataaaaa aatggcagaa gtcttattgc agatcacggg
     1801 tgtgctccaa acgattcctt cacttgcctt attaggttta cttatcccat ttgtagggat
     1861 cggaactgtt ccagcattga ttgcacttgt tatttacgct ttgcttccga ttttccaaaa
     1921 cacgtatatt ggtctagctg aaattgaccc gtctattgaa gaagcagcgg tagcatttgg
     1981 tatgtccaga atgagacgct tgctgaaagt agagcttccc atcgcattac ctgtcattat
     2041 ctcaggtatc cgaactgcct tagttttaat catcggtacg gctactttgg cagcattgat
     2101 cggagcaggt ggattaggga catttatttt gttaggtatt gaccgaaata cacctgtttt
     2161 gacattgatt ggtgcgatca gttctgcctt gcttgcaatt atattcagcg gactgattcg
     2221 ctggttgcag cataagaaac ctcgatattc catcattact ttagcagcag tggtgtgttt
     2281 atttggcggc gtttctttat atcagtctgc cttgtttaaa aatgaaacca tcacgattgc
     2341 tggaaaatta ggagcggaac ctgaaatcct gatccaaatg tataaagaat tgattgaaga
     2401 caacacaaaa acagaagttg aattaaaacc gaattttggc aagacgagtt ttttgttcaa
     2461 tgcactggat caaggtcaaa tcgatatcta tcctgagttc acaggtactg tcctagaaag
     2521 cttggtgaag gtacctgaag caatgaaaag tgctgatttg acaaaagaaa aaacatatga
     2581 cgaagcagaa gaattattga ataagcaatt cgatatgaca ttattaaaac caatggctta
     2641 ccaaaatacg tatgccttgg cagtcaagag atcttttgcg aacgaacatc aacttaaaaa
     2701 gatttccgat ttggcagcta tctctgatca gatcaaagcg ggattcacac tggaatttat
     2761 tgatcgttca gatggatata aaggaatcca agaagcatat ggcttgaatt tttcaagtgt
     2821 acaaagcatg gagccaagtt tgcgttacca agcaatcaat aatggagatg tcaatattgt
     2881 ggatgcctat tccacagata gccagctcaa ggagtacgat ctagtgactc tagaagatga
     2941 tcgagggtta ttcccagctt atcaaggcgc accgctgatg aaaaaagatt ttgctaaaga
     3001 acatcctgaa atcgttaaag cactgaataa attaagcgga aaaataacag aggatcaaat
     3061 gattgatatg aattaccaag tcaatgtcga aaagaaaaag ccgacagaag tagcacgaat
     3121 cttcctgaaa aatgaaggat tagtaaagga ggaagatcag tgactaaatt aatcgagttc
     3181 aaacatgtgc aaaaaaagta tgatggaaag tatgtcatcg atgacctaaa tctggcaatc
     3241 aaaaaaggag aaatctttgt tttggttggt ccttccggta gtggaaaaac aactactttg
     3301 aaaatgatca atggattatc taaaccatca gcaggagaca tctacttcaa aggaaaatcg
     3361 ttaaatgaat ataatctgca gaaaatgcgg tggaatatgg gatatgtgct gcagcaaatc
     3421 gctttgttcc cgaccatgac agtcaagcaa aatattgaag tcatccctga aatgttaggt
     3481 tgggaaaagc agaaaagagc agaccgtgta gatgaacttc ttcaaaaagt tggcttatct
     3541 cctgatattt atcgagatcg tatgcctaga gagttgtctg gaggagaaca gcaacggatc
     3601 gggatcatcc gagctattgc tgcaagccca gatgtgattt tgatggacga gccatttagc
     3661 gcattagatc cgatttcaag aaactcatta caagaattgg ttttgtcctt acacgaagaa
     3721 ttgggcacga ccatcgtttt cgttacgcac aatatggaag aagcaatcaa gctaggtgat
     3781 cgtattgcat ttatgaagga tggagaaatt atccaatgtg acacaccaga gcagttattg
     3841 atgaatccta aaaacgacta cgttcggcat ttctttgatg aaccgaaaca gacgaaagag
     3901 tggcgagtag aagatttagt cgtcaatgga tatttcttaa atgaaatacc agaaaatgct
     3961 cgtcaacaag tttgctttga tacgcctatg aaagaagtat attcgcttct ttcggtgtat
     4021 ccaagcattg tgatagtaga aaagcaaaga agtattgggt cgctgacaag taaagaaatc
     4081 tttgcttttt taagtcgaga ggaggaagga aatgaaaaca tatgatgcaa tcatcatcgg
     4141 ttcaggtgtg agcggcttaa gtgctgctta tggattgaaa gaagcaggta aaacagtact
     4201 agtggtcgaa gaagatctat ggggcgggac ttgtcctaac cgtggatgtg atcctaaaaa
     4261 agtcttgtta agtgcagtag aggcacgtaa tcgggtaaaa caattgagtg gaaaaggttt
     4321 taacgagatc ccaactgcca actgggaaga acttcaaaaa ttcaaacgaa catttacgga
     4381 tccagtccca gaaagccgaa aaaaacaatt agcagaagct gaaatcgatc atctttcagg
     4441 aaccgctcgt tttttggatg attcatctat tgaagtcaac gaagaagtct tccatgcgga
     4501 ttacttagtt ttggcaaccg ggcaaagacc tacgatctta ccagtggagg gaaaggaata
     4561 cttgaaaact agtgcagact ttttatcatt gcctgtactg cctaaagaaa tcatttttat
     4621 cggtggtggt tatattgctt ttgagctagc tacgattgcc aatgctgcag ggagcaaagt
     4681 aaccattgtc catcataatc aacggccatt aaaagaattt gaggcgtctt tggtagagga
     4741 agccgtccat caaatggaag catctggcat tcagtttgct tttggtgttg aaacccaaaa
     4801 gatcatttca gaaggtactc gctatcgttt agtcggaaaa gagacggaat tagtagcaga
     4861 tatgattttt tgtgcgactg ggagacagcc gaatacagaa tctttagcac ttgagcaagc
     4921 gaatatcgtc tttgataaac acggaattgc agtcaatgat tatctgcaaa cgagcaatcc
     4981 aaaaattttt gcgtgtggag atattgtttc aaggaaaacc ccaaaattga caccagtcgc
     5041 cacttttgaa ggaaattatg tagctaagcg gataacggat gcaacaagcg aacctatcaa
     5101 atatccgatt atacctacga tcgtctacgc cagtcctaaa cttgctgaag taggagttac
     5161 aaaaagtcac gcctcttcgt cagatcaagt ggtagagatg gatcttacca gttggttcac
     5221 ctatcatcgt gtcaatgaac cagttgctaa ggctgaattg acctttgacc agcaaaatta
     5281 tttgattggt gctgcagtga ttagcgagca agcagacgaa ttgatcgatg atttgacact
     5341 cgtcatcaat caaaaattga ctaaaaaaga gttggatagc tatatcatgg ggtatccgac
     5401 attggcaagt gatctctcct atttattgaa ataatcagca tgataactaa agcgcttgtt
     5461 gctttttcct tcttgtgcta cactttaaaa aatgaatacc tgtttctggg gatgtcgtag
     5521 acgacatgag agtaagttgt gaacttagac ccattgaacc tgtcagttaa tactggtgaa
     5581 gggagaaaca tagcagacgc ttgaagcggg ttactatgtc gtgcccattt caagcgtttt
     5641 ttgttattca tattagaaga aaggaggtaa caaaatgaag aaaaatcttt atccagcagt
     5701 cagtatggca ttcattttaa ttatttggca gattgttgtc atagtttgga aaatacctgc
     5761 ctttatttta cccggacctg ctgctgtatt ttctgcatta gcggcagatg cacaagtatt
     5821 actttcccat tctgtcacga cattaaatga agcagtgctt ggtttggtaa tagctagcgt
     5881 tctttctttt attactgcct tggcaatgga ttattggaag tttttagaaa tgtcttttta
     5941 tccgttacta gtgatttcac aaatgttgcc aatcatggtg ttagggccat tacttaccct
     6001 atggtttggt ttcggtatgc ttccaaaagt gatccttgtt gtcttgataa gctattttcc
     6061 gatcgtagtt tctttttcgg atacattgag gaaagtatca aaagagcaaa tcatcttttt
     6121 gaaaacgatg ggggcagata cagggaagat ttatcgaata tataaaatac cagcgggttt
     6181 atccggcttc ttttccggtt taaaagtggc tgctacttat tgtgtcagcg gagcagtcgt
     6241 tggagaatgg ctaagcgctc aagcaggctt agggtattac atgatacgcg taaaaaacag
     6301 ctaccagatc gataaagttt ttgctgcgat catttgtgtc attctattga gtttattgct
     6361 gaatggcgct tgttcattat tgaaaaaagg gtattatcgt atgctttata ttttttaaga
     6421 gaaggggatc aaatgaaaaa attatggctt ttattaccgc tactgttgct tttatccgct
     6481 tgtggcactg caaaagaaac aagtagcaaa caagaaataa aggatttgaa aaaagtgaca
     6541 cttgttttgg attatgtacc gaatactaat catactggca tctacctagc aaaagaaaaa
     6601 ggctattata aagaggctgg tttaaatgtc caaatcatcg aaccaggaga taattcgaca
     6661 agtattggtc tagtaggtgc ggacaaagca caatttggtg taagttatca agaagatgtc
     6721 acttatgctc acgcagatgg acagaatata ccagtcaaag caattgctac agtgatcaaa
     6781 cataatactt ctggattcgc caccctatca gacagtaaca tccattcacc aaaagatttt
     6841 gaaggaaaaa catatgcagg ttggcaaagt ccaagtgaag aagctgtctt aaaagctgtg
     6901 atggaaaaag acggcgggga cttttcaaaa ctgacaatgg taggaagcaa tggagaaggg
     6961 ccagaaagct taggaaaatc ctcagatatc caatggtact ttgaaggttg ggatatgatc
     7021 aaagcaaaag aagcgggtat tgaactaaac tatatcccat tgaaagaatt agatgaacgg
     7081 ttagattact atactccggt aattattacg aatgatcaac tgatcaaaag tgatccagaa
     7141 ttggtacagt cttttatgga tgccacgaaa aaaggctatc aggaagctat taaggatcca
     7201 aatgacagtg ctaaactgtt gcaaaaatat gcaaaagaaa atgatcggac gtttttagaa
     7261 gaatcacaag cattcttgtc aaaaaattat acggatgatc ctgagaattg gggattgatg
     7321 gaagaaaaag tttggcgcaa ttatacagct tttatgcagg aaaacggatt gatcaagcaa
     7381 gatgtacctt cgcaagaact attcactaat cagtttataa aataggagtg atagatatgt
     7441 caatccaaat cagccacttg aatgtagtca ttgaaaacct gaaagtatta gaagacatcc
     7501 atttcactat ccctaaagat acctttacat cgattgtcgc gccaagcgga gccggtaagt
     7561 caacattgct gaaaacttta acaggagtcc agcctatgac ttctggaacg atcaaagtag
     7621 acgatcaaaa agtaactggt ctccatacag catttagtta tatgccgcaa gaagatatgt
     7681 tacttccttg gctgagcgtc tatcaaaacg ttacgttata tcaaaaaatc aatcatctaa
     7741 caatcaatga agaacaagta agggattatc tggatatctt tgggttaaac ggctatgaac
     7801 attttttgcc ggaacaatta tctggcggga tgaagcagcg aacggccttg ttacgaacaa
     7861 tcatgaatcc ttcttcttac ttgcttcttg atgaaccatt tggagcgtta gatgctatga
     7921 ccagagggca gatgcaagac tggatgttga agctgcctaa aaaagcaaag cggactactt
     7981 tacttgtgac gcatgatatc gaagaagcaa tttatttatc agacaggatt cttgtgcttt
     8041 ccgcccgtcc ggcacatgtg attgctgaga ttcaagtgcc tgaaaaacga cggtcacgtg
     8101 aatggctact gcagcaatca gagttgaagc aaacgattta tcagttattg gcaggtgaat
     8161 tagatgttaa gtgatgctca ttgtcattta gatgggagtc agagtttagc actccttcag
     8221 caggaacaca cgattttgac gatcatcaat tgtgattcgc cagaagaatg gaaggaaaac
     8281 agacagcttg cagcaagtaa gactcaagcc ttaagttacg gtatccatcc ttggaaagca
     8341 gatagctata cgtttgaaca agttgagcct tttttgaaaa aagcaaggat catcggtgaa
     8401 atagggttag ataatatttg gacgaatgta ccgatgacta cacaaaaaaa agtatttgag
     8461 cgacagcttg cttttgcagc cataaatgag aaacctgttg tactgcacac aaaaggatgc
     8521 gaaaaagaga tcttaaatta tatccaagct taccctaatc actatttgat ccattggtat
     8581 tcttctttgg actatcaaca agactatatt gatttaggct gttatttcag cattggtctt
     8641 gatttgaaaa agaatccagc agtctggcaa ctagctaaag cagtcccaat cgaccactta
     8701 cttattgaaa cggatggact acatgcagca gagtgggctt taggggggat tttttcagaa
     8761 aaagattatg tctcactgct tgaagaacag ttaaagacaa tcgctgagat caaagagcta
     8821 tcagttgaag aagttaaaaa aaatacttat caaaacttgg aacgtttcat tagattaggt
     8881 aaataaaaaa gagatgtgac acaactagtg tcgcacctct ttttggcttt ctttgtataa
     8941 aagtaaactg tttgattaat aaatgaatct tagtaccaac cgtttgcttc ccagaatgct
     9001 ttcgccgctt cccaagaacc ataacgtgaa gtaacatatt gttctgctac tctttcttgg
     9061 tttgcagcag agtagtcacc atttaagtaa gaagaatcta attggtaacg tccgatgtaa
     9121 cgtccgttcg ttgctgtgta agaaccgctt gattcttttt gtgcgatcca ttcttttgca
     9181 gagcttgtgc ttgctggagt agcagcagtt tctgttacag caggagcagg ttgtgtttca
     9241 gcaacaggcg ctgcttgttt ttccgtttga accgtttctt gtacaacagg ttgttcagct
     9301 tggactggtg cttcttgaac ggctgcttga ggttcagcag ctgcttgtgc agagccttct
     9361 gttgggattg ttaattgtt
//
LOCUS       DACDPS010000090         9187 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.090, whole genome shotgun sequence.
ACCESSION   DACDPS010000090 DACDPS010000000
VERSION     DACDPS010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.090"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(789..1130)
                     /locus_tag="IWJ07_002313"
     CDS             complement(789..1130)
                     /locus_tag="IWJ07_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP9333853.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1130..1360)
                     /locus_tag="IWJ07_002314"
     CDS             complement(1130..1360)
                     /locus_tag="IWJ07_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP9333854.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            1647..1985
                     /locus_tag="IWJ07_002315"
     CDS             1647..1985
                     /locus_tag="IWJ07_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333855.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(2190..2765)
                     /locus_tag="IWJ07_002316"
     CDS             complement(2190..2765)
                     /locus_tag="IWJ07_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAP9333856.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(3374..3955)
                     /locus_tag="IWJ07_002317"
     CDS             complement(3374..3955)
                     /locus_tag="IWJ07_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9333857.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(4138..4764)
                     /locus_tag="IWJ07_002318"
     CDS             complement(4138..4764)
                     /locus_tag="IWJ07_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9333858.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LIESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(4786..6117)
                     /locus_tag="IWJ07_002319"
     CDS             complement(4786..6117)
                     /locus_tag="IWJ07_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAP9333859.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(6272..6673)
                     /locus_tag="IWJ07_002320"
     CDS             complement(6272..6673)
                     /locus_tag="IWJ07_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP9333860.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(6759..6965)
                     /locus_tag="IWJ07_002321"
     CDS             complement(6759..6965)
                     /locus_tag="IWJ07_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333861.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(7060..8562)
                     /locus_tag="IWJ07_002322"
     CDS             complement(7060..8562)
                     /locus_tag="IWJ07_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAP9333862.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(8580..9035)
                     /gene="argR"
                     /locus_tag="IWJ07_002323"
     CDS             complement(8580..9035)
                     /gene="argR"
                     /locus_tag="IWJ07_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP9333863.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
BASE COUNT         2578 a         1921 c         1475 g         3213 t
ORIGIN      
        1 aaaagtgact caaaataaaa aaaaccttac attatactgg aatgaactgt attgatagtg
       61 tacctaaaag ctctactgac ataagatcgt gtaatcggta aagtaatttt attaattata
      121 cgggatgtaa tcaattagag aataaactaa tcataaaagg tacttttaca ttcataattg
      181 tcgctttgag attctacttt ggttcactat ctgacccaat tgcacatttt cgatcattac
      241 aagtattggt gtcgttagaa caactaaata aaaactagcg gtagattttt cagaatctat
      301 gctaatttct gtatggatgt tccagtgatg gcaaatttct cttttcattg tttttcttgt
      361 cgcatgatat gcctttttgt gttattcagt taaactatag aaattctttc ctagacagat
      421 cttgatccca aattttacgt ttttgagcat cgatatagtt taaaatgaca ttactcatat
      481 ctaaagagtt tagatttttt aggactaaaa ctgtcagttg ttctttttct tctttctgtt
      541 aaagtattgt cgcgactttt cgtatacgtt aagatatgtc gtgaaaaaac aagttttgtg
      601 acacaaaaaa acgaatgact tgaggaaatc acctcaatca ttcgtttaaa atgctatata
      661 ttctttagtt taagatacgg aaattaatgc catcgaccac actagtagaa tgaacgacat
      721 ttacttggcc tttttttaat ttcgttatgt ttagcatata aaaatattat agagtttact
      781 tacaatgctt aaaacatata ctgtataatc tgatcaattt tagccatgtc ttgcaatggt
      841 aagttttcta tttttttgag ttttcgttcc ttaaaatcaa gagattttaa ttgtgtaata
      901 attacttgtc cttttatatc tagctcttca ggtaatgagt aacgtgtcgg taaattcttt
      961 atagtggaag taattggaca tatgaccgaa aacattgttt tacggttaaa ttcataacga
     1021 gagacgacta gtcctggtcg tcttttttga atttcttttc ctacggaggg atcaaaatca
     1081 atccatacaa tatcaccttt tttaggaata taaattcttt cttctgacac tataaaattt
     1141 ctctcccttc aggagttaaa tcttcccatt cgtctttctc ataatactcg gcctcttccc
     1201 caccactaaa tggatcttca atttttggaa ctaacgtaat tgttccatca tcagaataca
     1261 caacgatata ttcttggttt tctgaaggtt tctttccgtt atcagaaggt aaagttacca
     1321 ctacagaact tccttgtaat ctagattttg taacaagcat tatcatcact cccttaataa
     1381 aagtatacta tataacacta agtaacgcaa aattcccctt tgtaaactat ctagaaaatt
     1441 gttttgttta aagtatatct tattggatat aaatagaaat aaattgtagc cagttcattt
     1501 tttctttaac taaaaaaata atccaagaaa cattgaacct atggatccaa aagctaccca
     1561 aatactatag ctaacccccc gctaccttca attttgcaga taaaacgctt tgggagaaaa
     1621 tttcaaagac aacaggccat cctatcatgc ccgattataa acaaaaagct ggctattcct
     1681 tttgctctga caaatttcaa ttgggaaagt tcacgaaact agcaggtgaa gaagtttcaa
     1741 ctgttcggat caaagaatgc ccgcttcaaa tagaaacatt ggttcaacga atttcaacta
     1801 gacaagactt tgctattgta gaacgtgaga tcgtagctat ttttgtagaa gaagggattt
     1861 tacatgatcc aactcatatc aatgtggata aatggcaacc tcttatctat aaattcagag
     1921 aatatacaac tgccaaccag tcgttaactt tgaattttag attccaagaa ttcaaagagt
     1981 cataatgata aacatcgtta ggtataaata aaaaagactc gcagcacgag taaaaaacaa
     2041 atagaagcta tacattagaa aggtcgactg tgaccctata gatgcagtat atgcagctta
     2101 tgagcatttc ttctgttctc tttgtttttg ggatctgtgc tgaagtcttt ttcgtttttt
     2161 gccgctcttt ctttgaaaaa tcgattatgt tataccttct ccattactaa ttcaggcatc
     2221 tgctgagtaa tgatttttcg cgcaaaagaa atatctgtta cccaaggttc gatttgttct
     2281 tcagtcaaga caagcggcat tcggtcatgg attggtgcga ctgtgcggtt aggttcagta
     2341 gtcattatga ttgattctgc cattttttca tcctgatggc tacgataaaa accgccaata
     2401 tacactattt tttccttatt tgaaaacata aatttgtctt tttcttcatt ccactcataa
     2461 aagccactca ttgggaaaac acaacgattt tgcaaaaatg gttgatgaaa ggtctttttt
     2521 tcttctactg tctctgcacg ggcattaatc atcaattgtc cttttttaaa tccggtaaat
     2581 ccccacttca tgatttccgg agtgaggatg ttttcttcct cttttcttaa cgtcacaaca
     2641 agatttgacg gaaaaacttc cccagctgct aattctgctg cctgcttcgg ttgtttcgtc
     2701 tgggcttctc gataataatt ctgaaggagc tctgactcta gatcaaaaaa ataacgacca
     2761 cacatcctaa tccctcccaa tctttttttc tatcatacac tgcaacaaga agttcgcacc
     2821 aacaaaacgc tttcaatttt tgtagaacta aaaaagttgt gacaagtgac aaactctcca
     2881 gtgaatggta ttctcctgcc aactcctcgg tcacaagtca caatattcaa caaacatgag
     2941 aagctgcttt ttgaaattgt tccttgtgac tcggagtgaa acggcaggct tacgacctct
     3001 gggtaaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatat
     3061 gagcagctat ttttatttgg ctgttcttat tacttgatgc agggcagctt tttcacaacc
     3121 tctccactaa acggtgttca atcagctaac cttcggtatt agctcaaaaa acggcaaaat
     3181 atgaggaact attttgccgt ttgtttctct aatactcagg tcaaagcgct gatttcacaa
     3241 cctctaatac acggtgttcc agaagtagct tcttcgaaaa ataagccgaa atttcacaaa
     3301 aatttgagaa gcaattttcg taaattcctt cttatttctt gaagctgacc acttctgtca
     3361 caacctctaa ttattaaaga accttttgcg aagttaacaa tctttctttg atcaattgaa
     3421 aattctgcct aagtagtgat agatcctgtg tgactttcaa caataagata cttccctgga
     3481 tttcagcaat caacgctttg gcttgacggt ctgcttcttc atcggacagt ttttcttcag
     3541 aaagtgccag gatattttgt ttcgtatact tgacccattg attgactaat tctagtagca
     3601 acagacggta ttcttcatcc actgtactca actcaataat caaattacct acaggacagc
     3661 catgataagt gatctgtgtc tcatgatcag cgaaaatcca gtccaacatt tcttctaatg
     3721 cttcttctgg ctgttttcca gcatttaaga tgtcttcgaa caattctgtc tgccatacgt
     3781 ccatatgcgc atggatcacc gctagacaaa cttctttttt tgaagaaaag taataataaa
     3841 attgaccttt tcctgtatca gaagctgcta gaatatctcg gattgacgtt tcatgatagc
     3901 cttttttata aataagatct ttggtcgctt ccaagatctt ttcctttgtt ttcaatgata
     3961 ttctcctttt tcatttctca tagtcctata ttatacagta aattcatcat tttttcttct
     4021 taatcgttta aaaaagactg tgccaaatta tctgacacag ccctcactat aaaactgttc
     4081 tcatatagct agatgttcat tgattcaaga actgcgagga caaaagcaat tcctatctta
     4141 tgatagttgc tctaatactt cctctgttgt catcacttga tctgcaataa agccaaagtt
     4201 cgtcaacgct gcatccattg cttcttggcc aggagcacca attgcatctg tcgctaccac
     4261 aacattgaat ccactctcta tcaattcacg catatgggaa tcgctacaca agtttccgtt
     4321 cattccacca agaattactg tgtcgattcc attttttctc aattggagcg ccaaatcatt
     4381 gctttctggt ccaaagattt tatgaggaga gctgatgatc gtattttcat ccaaataagg
     4441 tttcaattct tcgataaaat cggctcctga accttcttcc actgattcat attgactttt
     4501 tctagcaaac atctgctgct caagcatcat atgttctcct gcaccgccaa attgccattt
     4561 atggtcatgt gggtaaaaat aatgaggaga aataaagagt ttgtatcctt tttcttttgc
     4621 ttgttttacc atttcaatca tattttcgat cgtattgtat ttttctaaga tatcttttgt
     4681 caaaccaaag ccaccgcctg ctggtttcaa gaactcgact tgcgggtcag tgagaactac
     4741 tgctgtattt ttattattga acatggtgga ttctccattc gttatttatt ttaatacatc
     4801 atttagagct tcactcattg tcggatgaga atagatttga tctctcaata acgtataagg
     4861 aagttttcca ttcatagcta gcgaaatcaa gttgatgact tcatgagaat cttccgcata
     4921 gatacttgcc cctagaatca agtcagtttc agggtcgact aaaactttga atacaccttt
     4981 aggatcttct aatacttgtg ctttagggac accgcttgtc atatacttga ataatttgta
     5041 attgacgcct tgtgctttgg cttgtttttc tgttaaacct acattggata aagtcggagt
     5101 gataaataca ctgtaaggca cagctttccg gttattcgtt gttcgtttat tctcgccctt
     5161 caactgatcc ataatgattc gataatcatc tagagagata taagtgaact gcaatccgcc
     5221 tttgacgtct ccaatcgccc aaatgttgtc tgcatctgta cgaaggtatt catccacagc
     5281 gattgctccg cgatctgtta gtttaacatc tgtattttct aatccaagtg atgctgtact
     5341 tggttttctt ccagttgcag ccaaaacttt tgacgcttgg attgttactt cttggccatc
     5401 ttctgtaaag gtgacggcag ctgttgattc ttggtctgct acttgatcta cagatacacc
     5461 tagatggaat tcgattcccg catttgtcat gtcttctaaa atcatttgcg cgatatcttc
     5521 atcttcacgt ggaagaaatt cagaatgggc atcaagaacg acaactttag atccgtattc
     5581 attgaacatt gatgcaaatt ccaagccaat atatccagcc ccgatgatca ctaatttttc
     5641 cggtaattgt tcctgatcca tcgcttgcgt agaatcaatc aagtagcgac ttgtattcaa
     5701 tcctttgatt ggtaaaatga ttggttcaga accagtattg atgaaaatcc gttcgccttc
     5761 aacaatcact ttttcaccat tattcgtcac ttcgatttga tggttagaaa gaaaacgtgc
     5821 tgtaccgtcc atcactgtcc cagttgcctc atcgctgatc atatgataat ttttggcgcg
     5881 aagcatgccc gtcaattttt ctttccgagc aacagcctct gtaaagtcga ttcctctttc
     5941 tccattgaaa atcaatgatt tagaaggaat acagccgatg ttgatacatg ttccgccata
     6001 catacgtgtt gacttttcaa cgactaaaac cgattcacct tttgaagcta acgccttcgc
     6061 taaagtctta ccgccctttc caaaaccgat cacaatattt tgatattttt ccatcatttt
     6121 tccctcgctt cttttttacc taccgattgg tacaaaaata tgataaccga taattgtcta
     6181 ttctccaaat catctgcttt atttgtcaaa aaaaaccaga caaaaagcgg tcctttcacg
     6241 tttttagtct ggttcgattt cctttattct tctaaacaat cgttacttct acatcgatat
     6301 ttcctctagt tgctttggag taagggcaga attcatgcgc atcttctgca attttttggg
     6361 cttcttcttc gctgatgcca tcgatatgca cttctaatac tacaccgatc tgatagccgc
     6421 catcatctaa attataaaga gagacacgtg cttttactgt ggactctgct gtcattcctg
     6481 cttctttgat catatgttgt accgccccat tgaaacaaga agcataacct gctgcaaata
     6541 attgttctgg atttgttcct tctgcatgga ttcctggagg agtgattttc atatgcatcg
     6601 agccatttgg tgcctcgact tctccattgc gcccgccatg gttgatcatt gctgtttcgt
     6661 atgctttttt cattgtactg ctcctttcga ttgaccattt gctacttggc tataattata
     6721 gtgtatgaca agtaaggcgc tttctacaaa aattctgctt attcgaatac cagaatgccg
     6781 tcttctacta tttttccaaa aaacaaaaaa atactactcg tgaacaacag cgtcatattt
     6841 gttccaaaat aaatagcaat cttttctaca taatctttta tatgattgat tttaagatac
     6901 atatatacca gaaagttgac cccataaacc aatactgtta aaaaaataac catattcgtt
     6961 accatttttt cctccttttg ctggtctccc taccagcact cgatttaacc ataccacttt
     7021 ttttgccgtc caagcaacta gcctttcaaa aagactggtt tattctgctg ttaatgattc
     7081 aagatcagaa aaatgtccga ataattttgc taattttact aacgccaact tgttctttcc
     7141 gaattcatca ttttctatct gtaatactag taacggctca tgtaagctta aacggatcaa
     7201 aaaccagccg tttccgtatg gctcttctag atcatatcga ataccttctt cattttccgg
     7261 gtcgaagttc atcccttttt cttcttccaa cagtcgcttc atttcttcta atatcttgtt
     7321 tccttgttcg cgtggttcat cgctgaacaa ttttaatcgg atttcttgta cttctacggg
     7381 ctgtttcaac tctttgattt gttcatctaa tgtgatacct tttcttttta attctggcaa
     7441 aagcatcagg atctttgcga tgacatatgc accatcatcc agaaaatagt tttccttgaa
     7501 tgcagcgtgt ccgcttgttt cgatggctag ctgtgtatct ttcccaagac ggttaagttc
     7561 taatgcttta ttgattacat ttcgatagcc ggaaatataa cgaacttgga tgcctccttt
     7621 ttcttcaatg aatctttgta gatgagaaga agtaggggaa ttcgtcacga ttcttgttcc
     7681 tggatgctct tttaatacga ttgtacttaa tactgcgatc aaattatttc gattgatcac
     7741 tgttccagaa gcactgacaa ctgcagaacg atccacatct gtatcgaaaa tgatcccaag
     7801 atctgcttca tttgctaaaa cagcctgctt gatactttcc atcgcttctt tattatcagg
     7861 atttgggata tgattaggaa atcggccatc tggttccaaa aattgggaac ctgttgaatc
     7921 cgcacctaag ctattcaata ctttttcagc aaagaatcca ccagcaccat ttccggcatc
     7981 aacaatgatt ttccaaccat ttagtggttt gctttctagt gttccaatcc cctttttaat
     8041 ttttgtgact aaatcttgcg cataaggtgt caaaaggtct gctttttcta cgcttccagt
     8101 ttgttcgctt tttatttttt gctgctgtga taggataaaa gcaatatctt ctttttctgc
     8161 tcctccttct gaggtaaaga attttattcc attataataa tagggaagat ggctagcagt
     8221 cagcataacc gcagcatcgc acgagaactg ttcaaattga gtggacataa acatagcagg
     8281 cgtagtagct aattcgaaat cgatgacatg tatcccttgt tctttcagac tttggatcaa
     8341 cgcctctttc agtgctggtc cgcttatcct actgtcatga ccaatcccaa tcgtccattt
     8401 tttatttgga tcggttgatt tcttcttttt ctttatccag tttgctagtc cccagccgat
     8461 cttttttacc atgtctgtcg tcaagtttac ttcatatcct tcaactggta atgcgattcc
     8521 tcgaatatcc gatccatttt gcagcgcttt catatctttc atataaaagt cctcctcatt
     8581 tattccctca ttttttccag cttttctgca aatcgctgtg cttcttcttc tgagcggcaa
     8641 ataataatga atgtatctac accggccatc gttccgacaa cttcagggta agccacttca
     8701 tctagccaat tcgttaccac atcggcacca tttcgttcag tcaatacgat aaccatgaac
     8761 tgaacagact ggatacgcaa aacctcgcgt ctaattgctg tacgcaaacg ttcttcattt
     8821 aaagaaacag atggctggtt aaataatgta tagcgtacta ttttgttttc attttgactt
     8881 tttactaact tcatttctcg aatgtctcga gaaatcgttg cttgagtcac atcggcgcct
     8941 gcttcatgca gccgcgcaag cagctcttcc tgtgtcccaa taggttgttc tgtaatgatc
     9001 tggcggatca gtgcttgtcg ctgtttcttt ttcatattag acacctctca atgttgatta
     9061 tagcaaaaga taagtaatat ttcttcatat tttggtattt tttactttta ttttttgttt
     9121 tcattcaggt tgctaacctt tttctatttt aacacgcaaa atcgtcttca ttttttcagg
     9181 tgctgat
//
LOCUS       DACDPS010000091         8879 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.091, whole genome shotgun sequence.
ACCESSION   DACDPS010000091 DACDPS010000000
VERSION     DACDPS010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8879
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.091"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..442
                     /locus_tag="IWJ07_002324"
     CDS             <1..442
                     /locus_tag="IWJ07_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333864.1"
                     /translation="SKTIPRYSKNKKAEKFCLLLNKFTLIAPILAFIIFSVLLSTTLK
                     GKFMERSSHAMILTFLWLLFTRIYIFLMSLKPPKSISLCLVINGIFLLSLIIFITPLD
                     RYVTYLYNPLEYWTYFIGILEGIIFYIGYFPNKNNNFYFYRNKL"
     gene            complement(568..1245)
                     /gene="nth"
                     /locus_tag="IWJ07_002325"
     CDS             complement(568..1245)
                     /gene="nth"
                     /locus_tag="IWJ07_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAP9333865.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1252..1950)
                     /locus_tag="IWJ07_002326"
     CDS             complement(1252..1950)
                     /locus_tag="IWJ07_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAP9333866.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2218..3402
                     /locus_tag="IWJ07_002327"
     CDS             2218..3402
                     /locus_tag="IWJ07_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAP9333867.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3408..3851)
                     /locus_tag="IWJ07_002328"
     CDS             complement(3408..3851)
                     /locus_tag="IWJ07_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9333868.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(4077..4364)
                     /locus_tag="IWJ07_002329"
     CDS             complement(4077..4364)
                     /locus_tag="IWJ07_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333869.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4535..5887)
                     /locus_tag="IWJ07_002330"
     CDS             complement(4535..5887)
                     /locus_tag="IWJ07_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAP9333870.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(6160..6606)
                     /locus_tag="IWJ07_002331"
     CDS             complement(6160..6606)
                     /locus_tag="IWJ07_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP9333871.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(6842..7768)
                     /gene="cysK"
                     /locus_tag="IWJ07_002332"
     CDS             complement(6842..7768)
                     /gene="cysK"
                     /locus_tag="IWJ07_002332"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAP9333872.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(7908..8795)
                     /locus_tag="IWJ07_002333"
     CDS             complement(7908..8795)
                     /locus_tag="IWJ07_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9333873.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYASVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT         2430 a         1804 c         1457 g         3188 t
ORIGIN      
        1 atcgaaaaca atacctcgtt attccaagaa taaaaaagct gaaaaatttt gtttattatt
       61 aaataaattt actttaattg ctcctatcct tgcatttatt attttttctg tacttttaag
      121 cactactctt aaaggaaaat ttatggaaag aagctctcat gcaatgattc tcactttttt
      181 atggttgttg ttcaccagaa tctatatttt tctgatgagc ctgaaaccgc ccaaatccat
      241 tagtttatgt ttagttataa atggtatttt tttattatca cttatcattt ttattacacc
      301 tttagataga tacgtcactt atttatataa tcctttggaa tactggactt attttatagg
      361 tatattggaa ggcattattt tctacatcgg gtattttccc aataaaaata acaactttta
      421 tttttatcgc aataaattgt aataagttac catcctagct actgagtatg tacatttact
      481 ttttcatgca aaaaagataa acgaaacagc ttttctccat aataacaaga taattcatac
      541 atttatttgc aattaaagaa accacgtcta caggcgtggt ttctttttta gcgccttttc
      601 ttttagacgc atccgatttt tcccatcttg acatattgat agcaaaggac atacctcgca
      661 cttgggattt cttgccgtgc aatgatatct accaaaaaaa atcagtgtat gatgtgtctt
      721 tacccataat tcttgtggta ctttccgcat cagtgtttct tctacttcca tgaccgtagc
      781 atctagtttg cagatcctca ggcgttttga gacacgttcc acgtgggtat ctactgcgat
      841 tgctggtatc ccgaatgcat cccccaatac gacatttgct gtctttctgc ctacaccagg
      901 aagagacatc aattcttctc ttgacgttgg cacttgccca tcgaaacgtt caatcagctg
      961 ctgtgcacac gctttgatgt tttttgcttt atttcgatac aaaccaatcg tttttatttt
     1021 aagaatgatt tcatcgattg aagcttctgc taacgcatcg ggtgtcggaa aagaggcaaa
     1081 caaatctggc gttgctttat tcactgatac atctgtcgcc tgggcactta agatcactgc
     1141 aatcaataac tcaaatggat tattgtgctt gagttctcca tgtgcttcgg gaaacattcc
     1201 atacatcgtt tctagagctt ccattgtttt ttgtttgctt aacatattcc gctactcctc
     1261 caaccaattt ttcagtgaga ctttcggcaa ttcttcttgt tgagctgact gttctacttc
     1321 tctttgaaga agctgctgtt ttcttcgttt ttggtcttct tccacttgtt gttttgtacg
     1381 tagatttttt ctttcccacg atagcaaaat gcggtcaacg taattaaaac tataagcctg
     1441 atttaatacg gcttcccgca gtgctagctt caaaatttcc ggctgataat ggtcttcttc
     1501 tagccattgt ccgatccgct ggaactcgat cgaagaaagc gggcgtccaa attcttgttc
     1561 aaataattga tacactgact ggatttcttt ttcttgtgtt ttttcttcgt attttttttc
     1621 ttgtgttttt aaatactcac ctaatagatc atagatgggg taaagattat atcgatctgc
     1681 ttttttaccg ttgacaatcg tcgtttctat cttgataaat ccatttgtta cgagacgatc
     1741 cagtatttga aaaatctgat cttgtttgat tcccatatcc atagcaatca tctgcaaatc
     1801 agggaaagta tctccttcaa gctgtgccat atgcaactgt agaacaaata aaaattcttc
     1861 agattgtaga ccgatcctat gataattttt aagtaaaaga ttagatatga ctgtttgtcc
     1921 agcgcttaag taattttcta aattcaacat gatttctcct ccgctcaatc acagtatacg
     1981 taaaaaaaac atgggaagca agaacaggtt tcttttctat ttacttcact gatcggtcag
     2041 atttacaatg tcgtctactt gatttttgtt tgttcatgtt tttgatgcgt atcttcactt
     2101 ttcggaataa ataaattaag tagatttcgc tgttccttag aatgttgaca aggaaacaaa
     2161 attactatac actatttttg tttccaagtt aacattattg cataaaggag tttttacatg
     2221 tacagtttca caaataaaca ttctaaagtt tttggatacg tctctgtctt attcactgga
     2281 ttaggattta ctatcgtcag tcctgtactt ccgtttttaa cgctgcctta ttctcactcc
     2341 atacatcagc aagctttcta tatcactttg ctcatgtctg tttatgctct agctgccttc
     2401 ttatctgccc ccattctcgg tagcttaagt gatcattttg ggcgtcatcc gatccttatt
     2461 atcagtttat taggatcaag tatcggatat ttgctttttg gtttaggaaa ttcgatatgg
     2521 atgttgttcc ttggtcgtat cattgaagga ttgacggcag gagaaatctc caccttatat
     2581 gcttactttg ccgacatcac ggaacctaat gaaagaacca aagtttttgg atggatgggt
     2641 gctttagtcg gtataggaac aacgctgggt cctattatag ggggtctatt agctgagttg
     2701 gggaattctg ttcctatatt tattggagca ctctttactt tcttaaatgc cgtttatggc
     2761 tacacattta tgctagaaag ccttccaata aaaaaacgtt cagtagattt aagtttctcc
     2821 catgtaagac cttttcacca attacaacaa ttattcaaga tttcatccgt tattccttta
     2881 ttgacagcag gttttgctgt ttggttggca gctggctctc ttcaatctat cttttctcaa
     2941 ttttcgattg acgcctttca gtggaaggct gggttggttg gtttaagctt ctctttgatt
     3001 ggaatcttgg atactgtctc gcaattgttc attatgcctc gattgctcaa aaaattttca
     3061 gaacagcaga tcacaagaat agggatgttc agtgaaatcc tcgcctatct attcatcaca
     3121 ttatctggaa ttcttctact gccgattttg tttttatttg ggattatctg ttatggtttc
     3181 ggtgattcta tttttactcc tgtattcaat ggccaactat ccaattctgt ctcagataac
     3241 cagcaaggtc ttgtaatggg cggtacccaa agtatacaat ctctatcgcg cgtcatcggc
     3301 ccgctaatcg caggacagct ttatgctgca gcaccatttc ttccgacggt attcggattt
     3361 ttgcttatga tttttgccta ttatcactac aataaaaaat agtagcttca tttgctactt
     3421 cgtataaatt ctctgtatcg accgataaaa cgaaatactg tgtcaaattc ttcttttgac
     3481 atattttcaa atacagtagc atctcgctgt acccattttt gatggagttg ctcgtgtcta
     3541 ttccttattt tttgtccgat tggcgtcaat tgaaaataga tttctttttt attctcttcg
     3601 ctttgataag tgtttatgat ccctctagct atcagttttt ttgttagctt actgatggct
     3661 cctctagtca tgtaactagc attcgctaat tttgtcacgt taggatcagg gattttcccg
     3721 atatgttcaa tcacttctag ttcatttaga gtaaatcctg caaattcttt ttccatttct
     3781 ggtttattac gatgaataat ctcattagct aaatccagat attcttctat tagcagttgt
     3841 cttttatcca tttctacacc ttctttttct ttcattatat tgtttttttg tttcctattc
     3901 aacaaagata aaaatatttt gtaggaaaac ccaatctgaa accaagaaaa aaaggggttg
     3961 acaaagcttg tcataacccc tttttctgat taaaagatag taaagagaca gtcatcggta
     4021 attgtgatga ttttttgtca tcacttaagg atgagcggcc ttttcaaaaa ctgtctttaa
     4081 agatcatcat cagtttctag cacatatggc tgaccttcat atgtgtactc tgtattcaat
     4141 gtgccgtcat tttcatctga gtagatgcgt ctactgaaag gaaaacgatg agaaacatta
     4201 tctttggcgt catttgctgg catcgtgtaa tattcctctt gcgtttcatt caactgagag
     4261 agtacttgat ctgccaactc atcaatattg atcgttaatt tcttcgaagc acttttataa
     4321 tcttctaaaa atgcatcttg ttggacaaaa tattctttat tcatcttctt tctcaccttt
     4381 cttttcaaaa tcaaataaag ctgcttgttc ttttatgtaa cagctttatc tttgctgctt
     4441 attgtgacaa aaaattcgaa gaaagacaac taattgtact tttcacaaaa aaagacagag
     4501 ggtcgtgaca aaattgtgtc acaacccttt ttctttattg tgcagtttcg atttcgccta
     4561 gcgctgcttg tgcaagtagg ttcaagtaat tccatgggcg gtcaaaatgt ggttggaaga
     4621 agaaatctga aagtgcgaga tcttctacag tcattttatt ttgaacagct aaagacatgg
     4681 tatttgctga ttgtgtaatg tcgtatttgg acatgaactg agcacctacg atacgattcg
     4741 ttcctttttc atagactagc tccatcatta ctttttcagt agttggcatg aactctggac
     4801 gataattatc ttcaaataca gtcgcttgga cttctagatc gttcaaagtt gcgctttctt
     4861 tggtcacacc tgtcgaaccg atcgtccagc caaataggta aagtcctgaa gttccttgag
     4921 taccacgata cgccatttta tgatctgtca agttatttcc cacaagcaaa ccttgacgaa
     4981 cggcatttgt tgccaaagga atatagtttt gcgtgccact tggattgtag ttgactactg
     5041 cagagtctcc agctgcaaaa atgtctggat tacttgtctg catgtattca ttcactttga
     5101 ttgccccgtt tggcagtgta tctacttttc cttcgatcag ttttgtattt ggacggaagc
     5161 ccacacataa aatgaccata tctgtatcaa atgtatctgt tggtgtagct acttgtttca
     5221 cttttccttc ttcatctgtg ataaattcag taacattttc gcctagcgca agagtaacac
     5281 cgcggtctgt caattctttt tctaagatat ctgtgaatgg tttatctaga tatttattca
     5341 agatgcgatc taatccatca atcaatgtca cttgttttcc agattcgaca aaagcttcta
     5401 ctaattcaat accgatatat ccaccaccaa caattactac acgttttgct tctttcgctt
     5461 gtgcaataat ttcatttgct tgattgtagt ttttgcatag gagtacattt ttcgcatcga
     5521 ttccactaat cggtgggata ataggccatg aaccagttgt catcactaat ttatcataag
     5581 aaaccgtttc tgtttctcct gttttcagat cttttgccgt tactgtttta ttttctgtat
     5641 cgacgtctgt tacatcgtgt tccattttaa ctgttgctcc taatgaagat aattcttcag
     5701 gatttgagta gaacaagcct gctgggtcct ttactactcc gccgacataa agtgcgatac
     5761 cgcaagataa gaaagaaata ttgtcatttc tttcatatac tgtcacttct gcttctggat
     5821 gattttttaa aatacttttt acagctgcag tacctgcatg agtacagcca attactacta
     5881 ctttcattta tggtccctcc gattaacatt ttgtccttcg acaaaactac tataacagaa
     5941 ataaaacgga tacaacagtt taatgcttgt gaattcacaa aggtttgttt gtagtttcat
     6001 tccttttcaa gcataagaac ccaatatcta caagattctt attttaataa tattcacttt
     6061 tatatctaag attgtgaata aaagacaaaa aaataaaaaa gctagcgttt tttttacaca
     6121 gctagctttt tttaggtata acacttgtag aaactgtttt tacatttgcg ccttttttgc
     6181 tgactcttta caatccggac agagtccata caattccaac cgatgttcat taatctcaaa
     6241 atcagtcagt ctgcttgcag ccatttctat atcttcaagc cctgggtaat gaaaatcaac
     6301 aatcttaccg cagttttcac aaattacatg ataatgacgc tttgaactga aatcaaatcg
     6361 gctagaagca tcgccgtact tcatttcttg aacaaatcca atcgaagtaa ataagcgaag
     6421 gttattgtaa acagtcgcaa cactcatatt tggaaaccga tgttcaagtg ctttataaat
     6481 ctcatcagcc gttggatggg aatgatgttc aatcaaatat tctaaaaccg cgtaccgttg
     6541 aggtgtgatt cgaatgttct ctttttttaa cttttcgata acctcttcga ctaacttatt
     6601 ttccatgtaa acaccccacg ttgttattct cgtcttatca tactattttt acattttaaa
     6661 atccacttaa catgaaaaga aaattcaatt ttattgtatt ttaatgaaaa aagacgtaaa
     6721 acagtcgcgt tcactattct acgtctctat tcaaataatt tttctcatca taaatttttg
     6781 tgtgttcttt tcatctttaa aagtttttta cataaaccta tctggagaaa aaccaagtgc
     6841 cttatatctc ttggtaaagg gctgtagata aataacgttc accattgtcg ggaaccacag
     6901 ctaatacctt cttgcctttt cctaattctt ttgcgacttt tagcgcggca gcaattgcag
     6961 cgccagatga gatgcctact aaaatccctt cttttcgccc aacttctcta gctgtttcca
     7021 tcgcttcgtc tccactaata gaaaggactt tatcatacac agaagtatcc agtgttttag
     7081 gaacaaaacc tgtaccgatt ccttggattt tgtgaggtcc cgcttctttc ccttctaaaa
     7141 tagcagattc tgctggttct accccgatta attcgatttt cggatacaca cgttttaatt
     7201 ctcggccagc gccagtgatc gttcctcctg taccgatacc tgcaacaaac gcgtccaagc
     7261 catttactcc aaatgcttga tgtatctcag gaccagtcgt cttctcatgc acaagaggat
     7321 ttgcttcatt ttcaaattgt aatggtaaaa agtagccgtt ttcttttgcc aaacgttctg
     7381 cttctctgat tgatccagaa ataccatccg caccaggagt caaaatcaac tctgcgccgt
     7441 accctttcat caataaacgt ctttcgatac tcatcgtctc aggcatcacg atgattactt
     7501 tatatccttt tgcaactcct accatcgaca atccgattcc tgtatttcca gaagtaggtt
     7561 caatgatagt atcacctggt ttcagtagac catcgtgttc tgctttttct atcatactta
     7621 aagcaatacg atcttttaca cttcctcctg gattaaaaaa ctcaagcttt acaaaaacat
     7681 ctgctgaatc ttctggtacg attttattta atttgacgat tggcgttttg ccaatcaatt
     7741 cggtgactga gttatagata gctgtcatct tcaattcctc cttgtctttg ctcttcttac
     7801 ttatcataat acaatctgtt catttatggc gagtgattcg tctttgacta gcaaaaaaaa
     7861 gacaaagata taccatatat ggtaatttct ttgccttttt atctaaatca aacgtttagt
     7921 tcttgaccaa ctaataaaga ataactggac atagacatgc catttttcgc cattagatca
     7981 tcaacagaca tgccaaattt ttgtgcaata tcccatagcg tatcacctga cacgactgta
     8041 tatgtctgac cattatttgt atttacatct gttgttgaag atgatgacgt tgaagtcgtc
     8101 gtagtagtcg ttgttgttgt acttgtggtt gttgtagccg aacctgttga tggcgtatca
     8161 aaacgggtta aattataagt attaatcaat gagttcaatt tggccccata atttggatcg
     8221 gtagcataac gacctgtcaa gtacgctgtt gcatcataaa aacttgttgt attgcttttc
     8281 cacacgctag cataatgata atcgcccgtg gaaaaactgg ttgaacgaag aacattcgca
     8341 tggtcttgga aggattccca ataagaagaa tattttcgga atggctcatt catcgttacc
     8401 cattgtccgt ttacatattc tttggttgcc atataaacga cctgtccgtt atagcttcct
     8461 ttcacaccga atagattata atttggcgca ctagctaatc cggaagtgcc atatgcactt
     8521 tccaacaacg cttgagcgat catgactgaa gcatacaagt cattacttgc agcaacttct
     8581 gacgcagacc agccgatttg atcgataaac gcttgaggat cagcgactgt ttgagatcct
     8641 tgttcatctg catgaacatg ttgcggtacc gctgtaacca tcatcggccc aactaaaaga
     8701 ctcgttccaa ttaaaggagc aatcgctttt ttattgatca ccatttttct tttaggtcta
     8761 tggtgccggg cactgcgggt cttcagctct tgcattcaaa ttcctcctca aaataaaaaa
     8821 acttaataaa aagttctctt cttaaaacaa acttaattaa ttaaattata cttttaatt
//
LOCUS       DACDPS010000092         8408 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.092, whole genome shotgun sequence.
ACCESSION   DACDPS010000092 DACDPS010000000
VERSION     DACDPS010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.092"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            371..796
                     /gene="lacA"
                     /locus_tag="IWJ07_002334"
     CDS             371..796
                     /gene="lacA"
                     /locus_tag="IWJ07_002334"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HAP9333874.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            813..1328
                     /gene="lacB"
                     /locus_tag="IWJ07_002335"
     CDS             813..1328
                     /gene="lacB"
                     /locus_tag="IWJ07_002335"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HAP9333875.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            1339..2271
                     /gene="lacC"
                     /locus_tag="IWJ07_002336"
     CDS             1339..2271
                     /gene="lacC"
                     /locus_tag="IWJ07_002336"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HAP9333876.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTISHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            2274..3254
                     /gene="lacD"
                     /locus_tag="IWJ07_002337"
     CDS             2274..3254
                     /gene="lacD"
                     /locus_tag="IWJ07_002337"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP9333877.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            3282..3599
                     /locus_tag="IWJ07_002338"
     CDS             3282..3599
                     /locus_tag="IWJ07_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP9333878.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            3599..5305
                     /locus_tag="IWJ07_002339"
     CDS             3599..5305
                     /locus_tag="IWJ07_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9333879.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTLLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            5447..6853
                     /gene="lacG"
                     /locus_tag="IWJ07_002340"
     CDS             5447..6853
                     /gene="lacG"
                     /locus_tag="IWJ07_002340"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP9333880.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            6876..7148
                     /locus_tag="IWJ07_002341"
     CDS             6876..7148
                     /locus_tag="IWJ07_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HAP9333881.1"
                     /translation="MLSDTLEKVQHLFRPGFEKVYIVSFEDCPMIPELEATPLLKCGK
                     WYVSTGKEWICHSDLELSAFEWEFLQSLDVEIRETIHFEVNYLPFQ"
     gene            7169..8068
                     /locus_tag="IWJ07_002342"
     CDS             7169..8068
                     /locus_tag="IWJ07_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP9333882.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSPLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
BASE COUNT         2673 a         1427 c         1731 g         2577 t
ORIGIN      
        1 tgatgaaata agaacagagg attgacgtat attagcttag gtcagatttt gtataagacg
       61 aaaataaagt aggacctctt aatcagtaag ttatagaaag taaaagactt ttgtaatact
      121 tgaatagata tttcacgtcc attttgtgat ggattaaatg aacaaaaatg aacaataatt
      181 taacggtgtt atctattttt taaaaaaaca aataaaaaaa acaaaaaata aacaaaaaaa
      241 gtgttgcttt ttgtttgaat gcttgatatt atataaacaa agaaatgatg aaaacgtttt
      301 ttcgaacatt ttgcaaaatg ttttctactt ctacgtagca tttctttcta aaatttagga
      361 ggtggtcaaa atggctattg ttgttggtgc agatctcaaa ggtacgagat taaaagatgt
      421 tgtgaaaaat tttcttgtag aagaaggttt tgaggtaatt gatgtgacta aggacggaca
      481 agattttgtt gatgttacct tggcagttgc ctctgaagta aataaagatg agcaaaactt
      541 aggtattgta attgatgctt atggagctgg tccattcatg gtagcaacta aaattaaagg
      601 tatggttgca gcagaagttt cagatgaacg ctcagcttat atgacacgtg gacataacaa
      661 ttcacgaatg attacggttg gcgctgaaat cgtcggtgat gagcttgcta aaaacatcgc
      721 taaaggtttc gtcaatggta aatatgatgg cggacgtcat caaatccgag tagatatgct
      781 taacaaaatg tgttaagaaa aggagttaag gaatgagaat cgcaattgga tgtgaccaca
      841 ttgtcacaga tgaaaaaatg gcagtatcag aattcttgaa atcaaaagga catgaggtcc
      901 ttgatttcgg gacatatgac cacgcacgta ctcactaccc tatctatggt aaaaaagtcg
      961 gtgaagcagt agtaagtggt caagcagact tgggagtatg tatctgtggt acaggtgtcg
     1021 gcattaacaa tgcagtcaat aaagttccag gtgttcgctc agcgttggtt cgtgatatga
     1081 catcagctat atatgcaaaa gaagagttaa atgcgaatgt tatcggtttc ggtggaaaga
     1141 ttactggtgg tcttcttatg aatgacatca ttgaagcttt cattgaagct gagtacaaac
     1201 caacagaaga aaataaaaaa ttgattgcaa aaatcgaaca cgttgaaaca cataatgcac
     1261 atcaagcaga tgaagatttc tttacagaat tccttgaaag atgggaccgt ggtgactacc
     1321 acgattaagg tataacaaat gattctgaca gtcacactaa atccttcagt agacatctct
     1381 tatcctctag aaacattgaa aattgataca gtgaataggg tgaaagatgt tagtaagaca
     1441 gctggtggaa aaggcttaaa tgttactaga gtcttgtatg aatctggtga taaagttact
     1501 gctacaggtt tcttaggtgg taaaattggt gaatttattg aaagcgaatt agaacaatct
     1561 cctgttagtc cagcttttta taaaatctca ggaaacacta gaaactgtat tgctatctta
     1621 cacgagggta accaaacaga aattttggaa caaggaccaa caatttctca tgaagaagca
     1681 gaaggctttc ttgaccacta tagcaatctt attaaacaat cagaagttgt gacaatttca
     1741 ggaagtttac cttcaggact tcccaacgat tattatgaaa aacttatcca acttgcttcg
     1801 gatgaaggag tagcagtcgt tttggattgt tcaggtgcac ctcttgaaac agttctaaaa
     1861 tcgtcggcta aacctactgc catcaaacct aataatgagg aactttcaca gttgcttgga
     1921 aaagaagtaa caaaagatat tgaagaactc aaagacgttc ttaaagagtc actttttagt
     1981 ggtatcgaat ggatagtggt ctcattaggt cgaaacggtg cctttgcaaa acatggtgat
     2041 gttttctata aggtagatat tcctgatatc ccagttgtta atccagtcgg atcaggggac
     2101 tcaacggtag ctggtattgc atcagcttta aatagtaaaa aaagtgacgc tgacttatta
     2161 aaacatgcga tgacattggg tatgttaaat gctcaagaaa caatgacagg gcatgtgaat
     2221 atgactaatt acgaaacact aaactcacaa attggagtaa aagaggtata aaaatggtac
     2281 ttacagaaca gaaacgcaaa tctttggaaa aaatttcaga taaaaacggt gttatctcag
     2341 ctttggcatt tgaccaacgt ggtgctttga aacgtttgat ggcacagtat caagatacag
     2401 aaccaactgt agctcaaatg gaagaactaa aggttttggt tgctgatgaa cttacaaaat
     2461 acgcttcttc aatgttgctt gaccctgaat atggacttcc agcaacaaaa gcattggata
     2521 aagaagcagg tcttcttctt gcctatgaaa aaacaggtta tgatacatca agtacaaaac
     2581 gtttgcctga ctgcttggat gtttggtctg caaaacgtat caaagagcaa ggcgctgatg
     2641 ccgttaagtt cttgctttac tatgatgtag acagctcaga tgaacttaac caacaaaaac
     2701 aagcttatat tgaacgtgtt ggttctgaat gtgtcgctga agatattcct ttcttcttgg
     2761 aaatccttgc ttatgatgaa gaaatctcag atgcaggttc tgttgaatat gcaaaagtga
     2821 aacctcgcaa agtcattgaa gcgatgaaag ttttctctga tccacgcttt aacattgatg
     2881 ttcttaaagt agaagtacca gttaacgtta aatatgtcga aggttttgct gatggtgaag
     2941 tagtttacag taaagctgaa gctgctgact tctttaaagc acaagaagaa gcgactaacc
     3001 ttccatacat ctacctaagt gcaggtgtat ctgctaaatt gttccaagaa acattgcaat
     3061 ttgctcacga ctcaggtgcc aagtttaatg gtgtgctttg tggacgtgct acttgggcag
     3121 gatctgtaga accttacatc aaagaaggtg aaaaagcagc acgtgaatgg ttgcgtacta
     3181 ctggattcga aaatattgat gaacttaaca aagttcttgt taaaacagct agtccatgga
     3241 ctgataaagt atagtaaaaa cataaaacgg aggatattgt tatgaacaga gaagagatga
     3301 ctctcttagg gtttgaaatt gttgcttatg ctggagatgc tcgctctaaa cttttagaag
     3361 cgcttaaagc ggctgaaaat ggtgatttcg ctaaggcaga tagtcttgta gtagaagcag
     3421 gaagctgtat tgcagaggct cacaattctc agacagctat gttggctcga gaagcttctg
     3481 gggaggaact tccatacagc gttaccatga tgcatggtca ggaccactta atgactactg
     3541 tcttattaaa agatgtgatt catcacctca tcgaacttta taaaagagga gcaaaataat
     3601 gcataaactc attgaactta ttgagaaagg gaaaccgttc tttgagaaaa tctctcgaaa
     3661 catctatctt cgtgctattc gtgatggatt tatcgctggt atgccagtca tccttttctc
     3721 atctatcttt atccttattg cctatgtccc aaatgcttgg ggattccact ggtcaaaaga
     3781 cattgagacc ttcttgatga ctccttatag ctattcgatg ggtatcctag cattctttgt
     3841 tggtggtact accgctaaag ctttgacgga ctcaatgaac cgtgacctac ctgcgaccaa
     3901 tcagattaac ttcttatcta cgatgctagc gtctatggta gggttcctct tgatggcagc
     3961 tgagcctgca aaagaaggag gcttcttgac agccttcacg ggaacaaaag gtcttttgac
     4021 agcatttatt gcagcctttg tgacagttaa tgtttacaag gtctgtgtga aaaataatgt
     4081 tactattcgt atgcctgaag aggttccacc aaatatctct caagtattta aagacttgat
     4141 tccattcact gtagcggttg ttctccttta tggatttgaa ctcattgtta agggaactct
     4201 tggtgtaacc gttgcagaat caatcggtac ccttcttgct cctctcttct cagctgcaga
     4261 tggttacctt ggaattacac ttatctttgg tgcttatgcc ttcttctggt ttgttggtat
     4321 ccacggtcct tcaattgtcg aaccagcaat cgctgctatc acttatgcca atatcgatgc
     4381 caacttgcat cttatccaag ctggacaaca tgcagacaaa gttatcactt ctggtactca
     4441 aatgtttatt gttaccatgg gtggaacagg agctacattg attgttccat tcttgttcat
     4501 gtggatttgt aaatcagaac gtaaccgtgc catcggacgt gcctcagttg ttccaacatt
     4561 ctttggggtt aatgagccaa tcttatttgg tgcaccaatc gtattgaatc cgattttctt
     4621 tgtaccgttc attttcgctc cgattgtcaa cgtttggatc tttaaattct ttgttgacac
     4681 attgaacatg aactcattct ctgccaacct tccatgggtt actcctggtc cgttgggaat
     4741 tgtactcggt actaacttcc aagttctatc atttatctta gccggactct tggtagttgt
     4801 tgatactatc atttattacc catttgttaa ggtatacgat gaacaaattc ttgaagaaga
     4861 acgttctggt aaaacaaacg atgctcttaa agaaaaagta gctgcaaact tcaatactgc
     4921 taaagcagat gccgttcttg gaaaagcagg cgttgctaaa gaagatgtag cagcaaacaa
     4981 taatatcaca aaagaaacta atgtccttgt tctttgtgca ggtggaggta caagtggctt
     5041 gcttgccaat gccttgaaca aagcagcagc agaatacaat gttccagtca aagcggcagc
     5101 gggtagttac ggtgctcacc gtgaaatgtt gccagagttt gacttggtta tcttggctcc
     5161 acaggtcgct tcaaacttcg atgatatgaa ggctgaaacg gataaattgg gcattaaact
     5221 tgcaaaaact gaaggagctc aatatatcaa attgacacgt gatggacaag gtgctcttgc
     5281 atttgttcaa caacagtttg attaataaat ttgtaaaagt tatttgagac gagattattc
     5341 tttcaattcc ttataatatt gttaaccata tcattgcatt tcatttcctt ggtttccgga
     5401 actcagaata tcgtctcaaa tgcttttttt gaaaggattt atagttatga ctaaaacact
     5461 tcctaaagat tttatttttg gtggtgctac agctgcttat caagcagaag gtgcgactca
     5521 cacggatggt aaaggcccag ttgcatggga taagtatctt gaagacaatt attggtacac
     5581 agcagagcct gcaagtgatt tttaccataa atatcctgtt gatttagaat tagctgaaaa
     5641 atatggcgtt aatggtattc gtatttcaat tgcctggtct cgtattttcc caactggcta
     5701 tggcgaagta aatgaaaaag gtgttgaatt ctaccataat ctttttgccg agtgccacaa
     5761 acgtcatgtt gagccattcg tgactttgca ccactttgac acaccagaag ttcttcactc
     5821 agatggagat ttcttaaacc gcgaaaatat agaacacttt gtagattatg ccgctttctg
     5881 ttttgaagag ttcccagaag taaactactg gacaactttc aatgaaattg gcccaattgg
     5941 agatggtcaa tacttggttg gtaaattccc tccaggcatt caatatgatc ttgccaaagt
     6001 cttccaatca caccacaata tgatggtttc tcatgcacgt gctgtgaaat tgtataaaga
     6061 taaaggttat aaaggcgaaa ttggtgtagt acatgcgttg ccaaccaaat acccttatga
     6121 tccagaaaat ccagcagatg ttcgtgccgc tgagttagaa gatatcatcc ataacaaatt
     6181 tatcttggat gcaacctacc ttggacacta ttcagataaa acgatggaag gggtcaacca
     6241 catcctagct gaaaatggtg gagaacttga tcttcgtgat gaagacttcc aagcacttga
     6301 agcagctaaa gatttgaacg atttccttgg tatcaactac tacatgagtg attggatgca
     6361 agcctttgat ggtgagactg aaatcattca caatggtaag ggtgaaaaag gaagttctaa
     6421 gtaccagatt aaaggtgttg gtcgccgaat cgcaccagat tatgtgccta gaacggattg
     6481 ggactggatt atttatcctg aaggcttgta tgaccaaatc atgcgagtga aaaatgatta
     6541 tccaaactac aagaagattt acatcactga aaacggtctt ggatacaaag atgagtttgt
     6601 agataatacc gtttatgatg atggtcgtat tgattacgtg aagaaacact tggaagtttt
     6661 atcagacgca attgcggatg gtgcaaatgt taaaggttac ttcatctggt cacttatgga
     6721 tgtcttctca tggtcaaacg gatacgaaaa acgttatgga ctattctatg tagactttga
     6781 cacgcaagaa cgctatccta agaaatcagc tcattggtat aagaaagtag cagaaactca
     6841 agtgatagag taatattact tgaaaatgta aaaaattgtt atccgatact ttagaaaaag
     6901 tacaacactt atttagacca ggctttgaaa aggtatatat tgttagtttc gaagattgtc
     6961 ctatgattcc tgagcttgaa gctaccccgt tgctaaaatg tggtaaatgg tatgtttcaa
     7021 ctggaaaaga gtggatttgc cattctgatt tagagttatc agcatttgaa tgggaatttt
     7081 tacaatcact cgatgtagaa atacgtgaga ctattcactt cgaggtaaat tatttgcctt
     7141 tccaataata aaaagaaagg aattagttgt gactatcgaa ttaaaaaacg agtatcttac
     7201 tgttcagttt aaaacacttg gtggtcaact gacttcgatt aaagacaagg atgggataga
     7261 gtatctctgg caagctgatc caaattattg gaatggacag gcgcctattc tatttcctat
     7321 ctgtggtagt ttacgaaatg attgggctat ttatagacct caagaaagac ccttttttac
     7381 aggtcttatt cgtagacatg gttttgttcg aaaagaagaa tttattctgg aagaagtcaa
     7441 tgataatagc gtgaccttta gtattaaacc aaatgctgag atgcttgata attacctata
     7501 tcagtttgaa ctgagagttg tttatacctt gaatggtaaa tcaattagaa ccgaatttca
     7561 ggtaacgaac ttggaaatgg aaaaaacaat gccttacttt attggagccc atccagcatt
     7621 caatagtcct ttagtagaag gtgaaaacta tgaggattat tatattgaat ttagtgaggt
     7681 tgagtcttgc tcgataccaa agagttttcc tgagacagga ttattagatt tgcaagatcg
     7741 gacacctttt ttggaaaatc aaaaaattct cgatttggac tactcacttt tttcccatga
     7801 tgccattaca cttgaccagc tcaagtctcg tagcgtaacg cttcggtcaa ggaagtctgg
     7861 taaaggtctt cgagtagatt ttgatgattt tccgaacttg attctgtggt ctacaactaa
     7921 taaaagtcca ttcattgctt tggaaccttg gagtggtctc tccacttcac ttgaagaagg
     7981 taatttctta gaagataaaa gacaggtaac gaaaatttca cctcaagaaa cttctagaaa
     8041 aatctatgat attactatcc ttaattaaaa tttgttatta tttgagtctt caataagatt
     8101 tgaatactct taccttgtga tgatagattg gtcttctccg tgccagtctt acatcgcaag
     8161 gtttttaagt tttttgtggg tctatgtcaa ctgtagtggg tgacgaaaag ctaatatcta
     8221 aagacagtct ggtagtaaaa aataagatta tttactactt cttgatttaa aataatagtg
     8281 gagccattaa tttgtggcaa atttaattta atattttgcc tcaaattagc cacacttttt
     8341 atagaactaa gaggaaaaat gaaattttct tcctattaat atagttgttt gaaatcttaa
     8401 atatagcg
//
LOCUS       DACDPS010000093         8374 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.093, whole genome shotgun sequence.
ACCESSION   DACDPS010000093 DACDPS010000000
VERSION     DACDPS010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.093"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            301..1740
                     /locus_tag="IWJ07_002343"
     CDS             301..1740
                     /locus_tag="IWJ07_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9333883.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            2035..2253
                     /locus_tag="IWJ07_002344"
                     /pseudo
     CDS             2035..2253
                     /locus_tag="IWJ07_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2502..2717
                     /locus_tag="IWJ07_002345"
     CDS             2502..2717
                     /locus_tag="IWJ07_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333884.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(2934..3190)
                     /locus_tag="IWJ07_002346"
                     /pseudo
     CDS             complement(2934..3190)
                     /locus_tag="IWJ07_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(3377..4288)
                     /locus_tag="IWJ07_002347"
     CDS             complement(3377..4288)
                     /locus_tag="IWJ07_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9333885.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(4307..5311)
                     /locus_tag="IWJ07_002348"
     CDS             complement(4307..5311)
                     /locus_tag="IWJ07_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP9333886.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(5308..7431)
                     /locus_tag="IWJ07_002349"
     CDS             complement(5308..7431)
                     /locus_tag="IWJ07_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9333887.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(7436..8356)
                     /locus_tag="IWJ07_002350"
     CDS             complement(7436..8356)
                     /locus_tag="IWJ07_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9333888.1"
                     /translation="MPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLY
                     GRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2393 a         1710 c         1548 g         2723 t
ORIGIN      
        1 atgaaaaaat tgggaaaaag tagaatggaa atttgcttac gtctcacgag atagctcaca
       61 ctattaatct gaagaacaat gaagtcgact tttagaaatt gctagaagaa agtaaagcga
      121 attatcacta gttactatag agtaagttca aataaagcat ctccaactta catggttaca
      181 tgaggatatc aaatcgaaat tactcaagct tcctatggct agagtcgcga gaagttgttg
      241 ggcagtagac ataggttata ttgttcttag ctacacgatc attagaaagg aaatcctatt
      301 atgtatagtt atcaagaaga tgcaaaagtc caaaaaaatc gctggtggat tttgattgct
      361 atatctatgt ttaccttcat gtccacattg gatggcagta ttgtcaatat tgcactacct
      421 accatctcag atgatatggg cataccaatg aatcaagcgg aatgggtagt gtccgtttat
      481 ctgatgattg tctgttcttg tttgttgctg tttggtaaaa ttggcgatag ccgtgggaaa
      541 atcaaagtat ttcggattgg tacgctgatc tttgtcttgg gatcgctgtt gtgtgggttc
      601 aatcattcgc taggtatttt attgttcgca cgggtggtac aagccattgg tgctagtatg
      661 actatggcta caaatgccgg aatcatcacg gaagtctttc ccatacatga acgtggtcgg
      721 gcattgggag caataggtgc gtttgtttca ttaggctcga ttgctggacc aggtcttggc
      781 ggcttgattt tgtcccaatt ttcttgggcg tacatttttt ggatcaatgt accggtggga
      841 atcgtaacga tgttgattgc tgaaaaattc ttaccaaaag atatcaccat gagcggccat
      901 acaatcgatg gagggggctt tgctgccatt gcggctacga ttatcagctt ttttggagca
      961 atctttttgg ggcaagaaag tggctttttc catccagtcc cccttgtact gttcgtaatt
     1021 tcagtgattg cctttgtcag ctttttaaaa gttgaaaaga aagcagcctc tccattgatt
     1081 tctttcggta tttttaagaa caagattttt accatgagct tgattacagc cttgttgatt
     1141 ttttcttcca acttctttgt caatgtagtg attccttttt atctgcaaaa tgctcgcggg
     1201 ttgagtccta gctatgctgg gctgttgatg atggtctttc ctttgatgat ggtcgtcggg
     1261 gcgcctttaa gcggctacct gaccgaccgg gttggcccgg agattttggt attagtaggc
     1321 ttgggcttat tgtcggtaac ccaggtcatg tatatgtttt tgtcagtgga ctctcctttg
     1381 tggctataca ttgtcgctac tgccatcatg ggtgccggca atgccttgtt ccagtccccc
     1441 aataatacga tggttatgag ctcggtcact atcgaaaact tgggactggc aggctcgatg
     1501 aattcctttg cccgaaattt ggggatggtc agcgggattt cgctttctac gacggtctta
     1561 tatcaagcga tgtctgctaa gatgggccag caagtaacta actatatcgc aaatcggccg
     1621 gatgtctttt tatatggaat gaagattacc tttttaggca gctttgtttt gtgttttgtt
     1681 gcctttttcc tgactatctg gcgtatcaaa aaacaaggag gtattcagaa aaatgtctga
     1741 aaaaaccact gttgaagaat ttttggaggc ctgcgtatgg gtgagtggga tttccatgga
     1801 tgatggaaga tgaagtgaac ttctaaaagc cgaaaaactc aagtattatc ttcaaaatac
     1861 tagtgctcaa tgagatgctt gatttgatca caagaagact gcgaaagccg ctggcaaccg
     1921 caagctaggt atcccaccga tggactacgt tttagaggct actggttatg aatacggagc
     1981 caacgctctc atctgcataa gaatacatca cccagattat gtaaagattt tcgatacttc
     2041 tgtcaaaaaa tcattaagat agataaatga tttccgtcta gtcaaattgg ttcagaatgt
     2101 agacacacag atggtaagaa gtcactcaca attcgagaat ggacttgtcc tatttgccgt
     2161 atacatcata atcgaaacgt caacgatagt atcaatattt tgaccaaggg ctatgaatac
     2221 aagcattgcc ttagaaaaca tcaagaacct taggaacaac ggggatagct tggttaataa
     2281 gagataccgc tgtttaggct tgcaagtata ctcttttatc aagaattccg tggctttagt
     2341 catgagaggc ttaatactat gaaaattttc tttagcattt ttgatttagg ttccttttct
     2401 aattataata agatgaccac tacagtaacc tactgaaata tataaacagc cactgtagta
     2461 ccattttcaa agtagtctat gagaggagaa tcttcattct tatgaacgct ggaattgcgg
     2521 atatgaattt aataaaaaaa acccttaatg attttacttc caattcaata tcaaaaggta
     2581 ctggtatcaa tttaagcaca ataaaaaagc tgaaaagtgg tgaaagatca gtggaaaagc
     2641 tgaatctttt agatgctata aaaataacgg aatttgctat gaaaaatgga aaagctgaga
     2701 ttgaaatatg gagataatta tcgtctagtg aattacttgc cacttagctg aatttaacga
     2761 tccttggtgc ttgaaggggg agattcttgg gaatagagca tacttgcaag cctatgtctt
     2821 tactaacaac gatatctctt attaaccaag ctatccccgt agttctaagg cttttacttt
     2881 tatctaagca ataatcgtat tcaaaggaaa tttttgaccc ttagaaagtc tcattttatc
     2941 agacgtgagc ttaggcattg ctgaattttg ttaattcggt tttaggttgt gctttgagca
     3001 taatatgaaa gtattcttta tcacgatttc attcatctaa ggtaatgtga tgtgattctg
     3061 aaattctttc aaaggcagtt tttgcataag ctgacattac atcatcaatg acatgtttac
     3121 aatgtttcac tactaaaata agggggtaac caagaagaaa aactgaatga ttatttgtat
     3181 ctaattccat ggtatatcaa ccatttccta tatagactga ttatgacatg gattaaaata
     3241 aaaaggcact atgtcttaat tgtcggtttt cgagttaccg aatggcattc atcacccacc
     3301 tatagaggat gggcgacttc tgcctgaaat aagttacact acttataact tctaataaaa
     3361 ttagattgag tcattattat ttaacatcaa cgattttcca gtttccattt ttttgtaata
     3421 ctaatttgta ctgaaagtag ctagtggttt ttgatacatt atcaaggtat tttatctttg
     3481 tactcactag aaaatttttt ccgttttgta gataaactgg attctctaac tccgaaaact
     3541 ttaagtcaat attgattggt cgcatcgcac cattttcaac ataatactcc aattcttttt
     3601 cagaagctga tggataaagt ttaaaaaatg tttctaaaaa atcggtgatt tcttccgttg
     3661 ttttggcatc aatttctgaa tcgttttcta cttgttttgc ttcatatttt gccttgtctg
     3721 gctttccaat cattgttggc aaactagtta ctaaatgatt cctgttttca tcttcaaaga
     3781 ttgtcactcg atatgcagaa gaaattgatt gcgtcttttt tccctctgta atatgttgct
     3841 ccactaaaaa tgaaacgaca aattcttccg agtgtttttc aacatctaga atttttacag
     3901 attgaacttc ggaactcgtg ggaatatctg ctcgaaccat atcttggatt aaagcgagac
     3961 cgttttctgt aaggtactgt tcgagattac tcattctctt ttcaattgct tctttgttat
     4021 ttttccatga aaaatattct ttcgcaaaat cctctgtaaa actttcaacg ccagaagtat
     4081 tcactacctt ctcttttata accacttttt catgagttgt atgttgatca atggcggtga
     4141 aatttttata aacgccaaaa gcaagactac aagacaataa aagccaaagg gcaagcacca
     4201 tttttcgatg cttacccacg gatacaactc gttctttttt cttttttggg ctcttttcct
     4261 tttgattccg ttcaattttt atcttcattc tttttcctct tttctcttat ttgtatcggc
     4321 cagctccgat aagatgctgc cgccaatagt tttccgttag attcgtccac cccactggat
     4381 ctcctgcatg aaacatgcga ttctcccctg cgtaaatgcc cacatgggtc acataagtac
     4441 cggcatcata ggttgaatga aagaaaacta aatcacctgg tttggcatct tttagatcaa
     4501 tatgagtcat ggcatcatat tgatcctgag ccacccgagg aagtttcagg tcaactttgg
     4561 cataacacca ttgggtcaag ccactacaat caaaccccgt ttctggactt gagccaccaa
     4621 acacataggc cgttccttca tacttaaatg cctcatcaaa aatttcttgg actattttgt
     4681 catcaaactg agttgtagtt aggtattgct tcactaataa acagtaaaac atattgccat
     4741 aattataccg ccacccacca ttttccttaa tcgcgattgg gttggggtaa tcgacttttt
     4801 gaccacctga tttttccttt gaaaaagatt cagctaaagc aaaactatgc ttcttcccgt
     4861 ttttcccgac atgatctaga tacccgccac catagttata ggcctgaata atactatctt
     4921 gatcacaccc tagttctttg gaacgcttaa ccaaagacgc aaaatagaga cagccttgtt
     4981 tgatggatgc ctcggtactt aatgaattag gtggtaatcc ggctgactca gaactttgca
     5041 taacgtcttc cattgtgcca ccagattcca cttgaataat cgccaaaaga attggaatat
     5101 actctgttat atccgattct ttggcatact tttccaccat tgcacggtgt ttcaatactt
     5161 cttctgagac actagttccg ccaggagaag tgccagaact accatttccc tcactgtctt
     5221 caccaaacaa caaacccaca cacatcaaaa tacccataaa agagatggtc agaaaagtta
     5281 atgaaataac acccactttc ttcaagttca ttttttctca cctgaccttg attttggacg
     5341 atggttcatt cttttttgta ctttttgatt ggtctgcttt ggcagagtgg acctctgttt
     5401 cacttgttgg aaagaatttt tcttacttgc tgattggctt ttcccttttg aaatgatttc
     5461 ttgactagtt agttttcgtt tgatttcggg attcagattt gctctcacaa tccgctttgg
     5521 tgcttctttt ttcagcatca tatctttctg aggttgagct gctttatcta ttggaatatt
     5581 cgcccttctt tttccctgac gatttgttgc ttcactcagt atcttgcgtt tttcgtccat
     5641 tttcgcattt cttcgttgtc gttgtttttc tcgcaaatcc actcgatccg ctttttcttg
     5701 gaacagtccg cgcttgaatt cttctggtgc ttttttcgac ttttcgattc ctttatggag
     5761 attgtatttc aaattcgttg gcgtattcct tacctgatcc tttacttgtt tagtcttatc
     5821 tttcaccaaa gcttgggtat ccaaaacttt gccgattttt ctcccagcca aattcattcg
     5881 ggattgtttc ttattatttg aaactggatt ctccttagtt aagtcagata gtatttcgtg
     5941 atcgttcggt aatcgtgaat tctttcgtag agaagaaccg gatggcttaa atttggattt
     6001 tgattgttcc tttgccaact tatttccgac tgctgcacca ccaccaaaag ccaaagctcg
     6061 tcccacattt cgattgagtt gccgcaattt tcgattcatt aacatttgcg gttttcgcat
     6121 gacgcgtctt cccattgact ggctatcatt actctggagg ttgaacatac tcataatgtc
     6181 gcccaatttg aaatagatac cggcaaaggt cacaatttgc agaaaagcaa ccatgaaaaa
     6241 tggataggtt gccgaaatat tgaaaaacat cgtggagatg ctaaatgcag tcgtaatcac
     6301 caaagtaaca cccgctcgca tcataatcgt attgaataaa cgaataatcg ccttctttcc
     6361 taaactttca taagtcggca acatggacaa taagaaacta attggcagaa acatcgcaaa
     6421 gataatgaag agaatttgcg agaacaacat aatccctgtt agaagaaaaa caaagaagga
     6481 aatgccaata ttgaacaata ccaaaaagac cacggtccct aaacggttca tggtcttggt
     6541 aatactgaga ttcgcattgt cattgtcttc aatctctgcc ttgaccgctt cttcccgatc
     6601 tttgcctttg ttttcatctg gactcacaga tagaatcttg ttgacccggt cttcaccaat
     6661 ttcttctatg ttggaatcat caaattgcag tagtaaccaa ggttgttgga cttgaatcga
     6721 aaacaaacta tcccgaatca aatccacact atcttttcct tggctttctg aattgggtac
     6781 aacgatctcg gttccaaggg taagggccgc ttcactaaca tctgagctaa agtcattgat
     6841 ttttgtgata taggtaggcg cataagcaat gaacgaaccc gatagtagaa aaatcatcac
     6901 aaaattaatc acggctcgaa ctgctttcgt ggtttctcgt tttaataatc cggtatacgc
     6961 cacatacacc cccagagcta aaatcatcaa taatagaaac ccaaaataaa atccggaggt
     7021 ctggagtcca ttttcagtaa taccagccaa tgtttggata ttctttccaa tactttcagc
     7081 cgtatccgaa atgaaatcta atttgtaagc ctcttgaacc acataacccg tggcgttcga
     7141 caaatacaaa ctcaccgtcc aaatgaaatt agtaatggca taaagaccat acatcacgct
     7201 tttccctaag ccatcacccc aattccaagg caaccaatcc caagagctat ccacaaagaa
     7261 atctagttga tagttgttca gtgaatattt ggaatataga tttcctgctt caactgtatc
     7321 atcaactagc cctgtcgcct cggctaccgt accaactaaa ctcaatagaa ctatcagagt
     7381 gagtatgcta ataaaaacga ttcccacaat ccgaaaaagt ttctttttca tcccctcact
     7441 ccattctctc ttgaacaggt ggacgggtat caaaggcatg gaagagttct tcaaagactg
     7501 gatgtacctg aaccacgcct acccgaccat acaaatcctg catcaagcat tggccatttt
     7561 ccaaatctcg taggcgtttt tgattgcttt catcttctcg atccaagcca aagaattcca
     7621 aggttttctt gatttcatga atatcggtac tgcgaaacgc aaacttcaac ccgatattat
     7681 tcttcaagcg ttcatccagt aaatcatcac tattttgcgt gacaaagtaa accccggcat
     7741 tcatagcccg cccagcacga acgagcttat tcgataatgt tttcccttgc gctacttgaa
     7801 gaaaactcca cgcctcatct aagtctacaa ttttgaagat ttctcgatta ctatggataa
     7861 agtccaaggc aaaagtggaa atgacgatta gcatggaaac actaagcaat tccatggtgg
     7921 tatattcttc aaaagtggtt tctgcatctg gtaaaaccaa atccgctact tgaatgatat
     7981 ttaactgcct atctaaacta atcgactggg taatcgaacc atctgagaaa agcaaatggg
     8041 caaaatcata atcggtaaag gactcgatgt gatccgcgat attttccgca actggtgagc
     8101 cttcttttcg aagctcatta attacttgta ataatccccg ttgattgctt tgggtaaccg
     8161 tccgaatggc cttacgcaac actggaaact tctccccatc tcgactagaa attcctgtta
     8221 aaaaggtcag aatatcaatt gctagacttt cagaatcctt ctttttctcc ataatcacat
     8281 aaggatcaag taatccttga ttttttggct tactagttaa gttgagaata ttgatttcat
     8341 ccgcaatttc tggcaaggtt tctttccagc cacc
//
LOCUS       DACDPS010000094         8228 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.094, whole genome shotgun sequence.
ACCESSION   DACDPS010000094 DACDPS010000000
VERSION     DACDPS010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.094"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            328..1251
                     /gene="truB"
                     /locus_tag="IWJ07_002351"
     CDS             328..1251
                     /gene="truB"
                     /locus_tag="IWJ07_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAP9333889.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1255..2196
                     /gene="ribF"
                     /locus_tag="IWJ07_002352"
     CDS             1255..2196
                     /gene="ribF"
                     /locus_tag="IWJ07_002352"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAP9333890.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2591..3376
                     /locus_tag="IWJ07_002353"
     CDS             2591..3376
                     /locus_tag="IWJ07_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9333891.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3389..4240
                     /locus_tag="IWJ07_002354"
     CDS             3389..4240
                     /locus_tag="IWJ07_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAP9333892.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4364..4816)
                     /locus_tag="IWJ07_002355"
     CDS             complement(4364..4816)
                     /locus_tag="IWJ07_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9333893.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
     gene            complement(5281..5649)
                     /locus_tag="IWJ07_002356"
     CDS             complement(5281..5649)
                     /locus_tag="IWJ07_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAP9333894.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            5799..6281
                     /locus_tag="IWJ07_002357"
     CDS             5799..6281
                     /locus_tag="IWJ07_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAP9333895.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            6318..7328
                     /locus_tag="IWJ07_002358"
     CDS             6318..7328
                     /locus_tag="IWJ07_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAP9333896.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(7443..8156)
                     /locus_tag="IWJ07_002359"
     CDS             complement(7443..8156)
                     /locus_tag="IWJ07_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333897.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT         2784 a         1337 c         1667 g         2440 t
ORIGIN      
        1 ttaagaaact gaaaaaataa ataaaaaaaa acggaaaaaa gactggaaga atggacagtc
       61 ttttttttcg tttttgggaa gtgaaaacca aactatttac ctgtaagcca cccataaatc
      121 caatcaaatg gtgcaaagaa aaaatcttcc attctattcc ctcctttaaa aaaaacataa
      181 catgatttct atttacgtga gcatgaattt tgttacgttt ttattgccga aaaataaaaa
      241 ccgttcatta tgatgtcaaa ttcgtcagta attgatgcaa tgtgttataa taattcagtt
      301 aaatcaaaaa tgaaatggag aaaagacatg gacgggttat taactttatg gaaagaacgc
      361 ggaatgacaa gtcatgattg cgtattcaaa ttaagaaaaa tcttacatac gaaaaaaatc
      421 ggacatgggg gtacgcttga cccagacgta gacggtgtgt tgcccatttg tattggaaaa
      481 gccacaaaag ttatcgaata tttgacagat tctgggaaaa tctatgtagg cgagataact
      541 ctaggtttca gtacaacgac ggaagatcga tccggagaac gggtagaaac aaaaccagta
      601 gatatgtctt tatctgaaga aaaaatagat gaagtgatgg cttcatttat tggtgagatc
      661 acccagatac caccaatgta ctcagcggta aaagtcaacg gtaagcggtt atatgaatat
      721 gcccgcaata atgaaacagt ggagcgtcct gttagaaagg ctcagattta ccgttttatc
      781 cggacaagtg aattgacaag agacgaagtg acaggaacca tttcttggcg cttcgaagta
      841 gaatgcggga aaggaaccta tgtccgcaca ttagctgtag atacaggcaa gaaacttggt
      901 taccctgcgc acatgtctga tctgacccga acagcaagtg ccggtatgtc ccagaaagat
      961 gctctgacat tagaagaagt agctcaagct gtgaaaaatg ggacaatcga tgattgtttg
     1021 ctgccaatag aaaaaggagt gaaacgattc aaacgagtag atattactga tgaagtatgg
     1081 cagaaagtaa aaaacggtat gagacttaat tatactgtat ttgcgctccc agaaatgccg
     1141 tcagaagaaa ttgctttgtt ttataaaggc aaagttgtca gtatctatca gccaaacccg
     1201 aaagaaaaaa acaaattaaa acctagtaaa gtattgagaa atgaggaata acgaatgaat
     1261 atcatcaaga tcagacatcc ttaccaatca gaacaaattc cagaagacga tgtcgtttta
     1321 gtactaggat tttttgacgg cgtgcatatt ggacaccaga aagtcataaa gacaggtatg
     1381 gaaattgcac aaaaagaagg attgaaatta gcattgatga ctttcaatca gcatccatcg
     1441 attgtcttta aaaagatcga tcctagcagc gtgaaatatt tgactagcct taagcaaaaa
     1501 gaagaaaaaa tggccgcttt gggtatagat tatttatatg aagttgattt tacttcttct
     1561 tttgctcatt tagcaccgca ggaatttgtt gatcaataca tcgtagggtt acatgcaaaa
     1621 tatgctgttt ccggttttga ttatacgtat ggaccaaaag acattgcaga tgtcgctcat
     1681 tttcctgaat atgccaaagg aagattcaaa atcgtcacag tttctaaaga agaagaacaa
     1741 ggtgaaaaaa tcagctctac ccgtattcga ggattactag acagcggaca agtaaaagaa
     1801 gcagcaaaac tattaggttg cacatatgag gtagaagggg tagttgtcca tggcgatgca
     1861 cgaggcagac tactaggatt ccctacagca aatgtcaaag taaagagcac agttcatttg
     1921 cctaaagaag gtgtttatgt atcggaaata aaaattgggg aaatttggta tccagccatg
     1981 ggttcaattg gacataatga tacattcggt gaaggacggg agctgactgt agaattgtat
     2041 attttggact ttaatcaaga tatctatggt gaacatgtcg cagtacgctg gaatcaattt
     2101 ttaagagatc aagtgaaatt ttctagtgta gaggaattga ttgaacaact caaagaggat
     2161 gaacaagcaa cagctgaata tttttctcag caataaaaca ttatgcataa aatctcttgt
     2221 ttatgtataa ctagcaattt caataaaatg ggtgataaat aagcccagga aaggggctgt
     2281 gacaaaagtc ttgtcacagc ctttttttcc aaataaatgg tttttcagaa gtagcttctt
     2341 cggaaataag ccgaaatttc acaaaaattt gagaagtaat ttttgtaaat tacttcttat
     2401 ttcttgaagc tggccacttc tgtcacaacc tctttcttta tagaaataaa aggacactag
     2461 caattcatga aaacgattgc taaagcgtat catttgcttt agactatata tcttggtgta
     2521 cactcacttt aagacggaaa taaaagttat ccgtaataac aggtttaggt atgaaaaagg
     2581 agtgggtact atgtataagg aattagaaaa caaagtcgca gtagtaactg gtggctcaaa
     2641 ggggattgga acagcgattg ctcaacgatt cgggaaagaa aaaatgaatg tagttgtcaa
     2701 ctataactcg gataaagaag gagcagaaaa agcagtcaaa gcaatcgaag aggcaggcgg
     2761 aaaagcagtc tctgtacaag cagatgtcag ttctgaagaa ggaatacagc gtttacttga
     2821 tacggcttta gaaaattttg ggacactgga cgtttggatt aataatgctg ggatggaaaa
     2881 tcagcatcca acccatgaac ttagccttga agattgggaa agagtattaa aagtcaattt
     2941 gactggcgtt tttatcggaa cgaaagcggc tttaaattac tttacagaga atgataaaaa
     3001 aggaaatatc atcaatatgt cttctgttca tgaacaaatc ccatggccaa cgtttgctca
     3061 ttacgctgct tcaaaaggcg gagtaaaact attcaccgaa acggtagcaa tggagtacgc
     3121 accaaaggga atcagaatca atagtatagg acctggtgct atccgtacac ctattaatgc
     3181 ggcaaaattt gatgatcctg aacaaaaaga actcttggaa agtatgatcc cactaggacg
     3241 cgtaggtgaa ccaaaagaaa tagcggcgac agctgcatgg ttggcttcag acgaatccag
     3301 ttatgttaca ggtattacac tatttgcaga tggtgggatg actttatatc cttctttcca
     3361 aggtggaaaa ggataagggt gaataaaaat gggtttattg attgcattga tcccggcagt
     3421 tgggtggggg atccagccac tgattttgaa aaagattggt ggcagaccaa caaatgagat
     3481 attaggaact ggtattggag ccgtgctcgt tggcttactt gttcaattat tcgcttctcc
     3541 agctggtatt tcagtgacta catttttgat tagtttactt tctggtgcat tctgggtcat
     3601 cggacaaaca ggacagtata catctttcaa cttgattggt gtttctaaaa cgatgccaat
     3661 ttcgactgca ttacagttag tcggaacctc attgattggt gtcttcgctt ttggagaatg
     3721 gtctggaagt atgggaaaaa tcattggtgc agcagcaatc attcttcttg tatttggttc
     3781 tgctttaacg gcaatcagtg atgatcctgg aaaaaaaggc ggtatcacaa aaggaatcag
     3841 tctattagca tcaacaagta ttggttattg ggtttacagt gcattgccca aactagttga
     3901 tgcaagcgga ctttccattt tctttccgca aatgatcggt gtctttttag gggcagtgat
     3961 ttatgtgatt ttccgaaaac caaccgcatt ttctgaagac aagagttgga aagcaacaat
     4021 agttggagcc atcttcagtg tgtcagcatt ggcttatatt ttttcagcaa atgaaaatgg
     4081 ggttgctact gcatatatca ttacacagct aaacgtagtt atctctacat taggcggtat
     4141 gtacatcctg catgaaaata agacaccaaa agaaaaaaaa ttaacgatta tcggattatt
     4201 gctgatcgta gtaggaagca tggtcacggt ctttatttga ggataaagat ggtaaaatct
     4261 catttattaa aaaaatattt gtttgaacca aacagtaagt acttataaaa aaatagcgct
     4321 agaatttcca acatctctgg aatttctagc gctttttaat tgtttaagat acaagtgaaa
     4381 tatctgtctg catcaataat ttttcattga tccattgact aaaatttacg acatgattga
     4441 agttaccacc gattagtatc tccgcatgat tgtctttata aagatcgtat tgtaaaagac
     4501 ggtctttttt atttgcgtaa acatcgttat ggtactggac taattgttct cggatttgat
     4561 tgattgctgt aaaatcacga tactgaaggt tgaaacgcat cgtgattggt gcatttagca
     4621 tgaatcgctg catttctttt gctgcttcct ctgcttgatc tccgtatagg aactcaaaaa
     4681 gattacgttc agaaaatttc cattgcccgc caacctttgt tccttttagt tttccttcat
     4741 gaagataatt tctgatagaa cgtgtagtaa cacctaattg ttcggctaat tcatttactg
     4801 agtaaaaatc tttcaatgtt caccctccta tgtgaatggt tctactgact tagttgatat
     4861 ttgcttaatc acgaaatgta gaaagaacga ttttaattaa ggtcattgta aaaatcaagt
     4921 cagaaaattc taacttttct ttctctaaag aaattcgttc tctcctaaag tgatattata
     4981 tttaaatata cctgaaaata atgggcagta ttatgaaaga tcactgatat tttctcgtat
     5041 tttttcctta ttcaaaagtt ttgcaggaaa attgctgaaa aaataatttt actaagtgga
     5101 aaattcaaag ccattcaatc agtgattttt ttcagcacaa gaaagactcc ttataaaaat
     5161 agcagatact ttgtcaatta agtgtttaac cagggatgat aaaaaggata ggattgaaca
     5221 aaacctgtat aagctgatga atagtttcct cgccagtaaa aggtttattc gtataaagct
     5281 ttatcgccaa aatttatcat gacctttttt atcttcaata ttaaatgaaa tcaattgttc
     5341 aatcaattcc cagttgatta cacttttcca aggaactttg atgacgttat ccgtatatac
     5401 gtagcctgct tcttggatat ttttttcaag ggcacgaatc gctgcttttt ccggtgcgat
     5461 agaaaaatga tttttagcaa aactaaatgc aataataaag gtaccatggt cagtaaacat
     5521 aggttggttc cattttacag ttgtgtctag ctggggatac gtttctgtta tcttagcaaa
     5581 aaggtctttg atcttttgtt gatgttcttt ttctttgatc tgattgatga tcggctgaaa
     5641 tttttccatt ctaaaacgcc tccattaagt ggtttgattt attttaccag aaaaacaata
     5701 gttatgtata aaaaacgaac aaaacacaag taatgttgta accgctatat ttatatgata
     5761 agatgtctca aataataaaa tagaaaagag acataaaaat gattgacttg gcaaagaaat
     5821 tcttacacga aaatatatat tttgagttgt atcatcatcg agccatttat acaaatgaag
     5881 atgctttggt aataaagaaa gaacaaggat ttacaggtac agagaccaaa agcttattct
     5941 taaaagataa aaaagataat aactacgtat atctgacttt tactacgaaa caaacggatt
     6001 tcaaagcatt agggaagatc gtcggtaaaa ggctttctat tgtttcatca gaacgcatgg
     6061 aagaacaaac gggacaaaag cagggcgctg tttctccgtt tggttatgaa aaacagattc
     6121 cgattattat tgatgaagag ctcttatcaa atgaaaaact tgtttttgca ccaggtagac
     6181 cagatcaaac gatggtcatc caaacggttg acttaaacaa aatcatccag ctacttgaga
     6241 tgccgatatt tttgatgccg gcaaacgaaa aaaacaatta accaagtaaa atagtgcata
     6301 cttggaggtg aagcaacatg ataagttatc cattattaga aagaaatgca acgattgggg
     6361 ttactgcacc atcctcaggc gtatcagagg aacaccattc gttactcgaa tcagctttaa
     6421 agagaatgga agaaaaatct ttccaagtag ttactggaaa gacgctttgg acgcaagata
     6481 aggaaaaatc aggtccagca agaaaacgag cagatgaatt caatcaaatg atgcaagatg
     6541 aaaatatcca aatgatcttt ccgccttggg gaggagaatt attgattgag attctcgatt
     6601 ttgtcgattt tgatgctcta caaccaaaat ggatagtagg ttattcagat attagtacgt
     6661 tgctacttgc tgtcacatta aaaacaggga tagccactgc acatggaacc aaccttattg
     6721 atttaagagg agagtacttg gatgaaacga cagcaatgtg ggaaagtgta ctgaaaacaa
     6781 gagaaggaga atcgattcta cagcaacctt ctgaaaagta tcaaaaagag tggccccatg
     6841 agcattcatc atcttctgtt ttccatctca ctgaaccaac aaattggcaa tcagtcagcg
     6901 gagatgcagt aaggatgaag ggacgattat taggtggctg tattgatacg atcagtcatt
     6961 taatcggtac cccttatgga gatgtccaat cttttaaaag aaagtataat gatggaaagc
     7021 cgatcttgtg gtttctagaa aattgtgagc tatcgtcagt tgatttaaga agatcacttg
     7081 ttcagatgaa actggcagga tggtttgatg gcagctcggg gatcatgttc ggtagaagtg
     7141 cagcagagga tttgttagat tataaaatga aggatgtata tgaagatctg tcaaaggaat
     7201 taaaactacc gatcttgtat gatattgatt gtggtcatgt accgccacaa ctgacattga
     7261 tcaatggtgc ctatgcagaa gtagcggtag aagatagaaa agcgactgtt ttgcaaacgt
     7321 ttatttaaaa aattttttcc tgccttggag tttatcaaag accaacaaat ctaaatagaa
     7381 aagaaaatga aagaagctga cagtattttg ccagcttctt tttgtatgat ctattagaaa
     7441 ttttaattat ccataactat cacattgatc agctgtttca catcatttgg attacctgta
     7501 ggaatagcta ctacagatgc attatcaata atttcttgag ttgtttctct aatgaacgct
     7561 ggataagatg tttgatctgc aggcatttgc caatgtgaag gaactggata actagtgatt
     7621 gtcccgtcat ggttacttga atacactaaa actccctggt aactgtattt cccagtgagc
     7681 ataaccacat ctttcggata gactgcactc cctgcatcat aaggggcgat aggtgttcct
     7741 gcaggataat gaataacatg tagttcgaaa tcacccgatg tatctctaaa agtattacct
     7801 ctaattgcta gccaaacacg tgcgtactca atttgtagat cagaatatcc tgctaacgga
     7861 tctattgact gagaagactg tgaggaagtt gtgtctattg ttgaagaaga aatatttgtc
     7921 tcaactgtac tactagatgt agaagaagta ttgggctctg agactttact gcttgttgaa
     7981 gatgtattcg tttcagtaga agctgatttc tgagtacttg atttttcagc tgaactggtt
     8041 tgtttgattg aacttttgtc tgtggtcgca gatggatcac tattttcttt actaggagac
     8101 gtacatccta caaggaataa agagaccaac aataaaataa aacctaactt tttcatataa
     8161 aatacctcat tattttttta ataccagtat agcataatcc tgaatttttg aatccgtaaa
     8221 accttgtc
//
LOCUS       DACDPS010000095         8050 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.095, whole genome shotgun sequence.
ACCESSION   DACDPS010000095 DACDPS010000000
VERSION     DACDPS010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.095"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..769)
                     /locus_tag="IWJ07_002360"
     CDS             complement(<1..769)
                     /locus_tag="IWJ07_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HAP9333898.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNAL"
     gene            complement(974..1324)
                     /locus_tag="IWJ07_002361"
     CDS             complement(974..1324)
                     /locus_tag="IWJ07_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333899.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            1919..2365
                     /locus_tag="IWJ07_002362"
     CDS             1919..2365
                     /locus_tag="IWJ07_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP9333900.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
     gene            2989..3981
                     /locus_tag="IWJ07_002363"
     CDS             2989..3981
                     /locus_tag="IWJ07_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9333901.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            3997..4881
                     /locus_tag="IWJ07_002364"
     CDS             3997..4881
                     /locus_tag="IWJ07_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018165023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9333902.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            4930..6537
                     /locus_tag="IWJ07_002365"
     CDS             4930..6537
                     /locus_tag="IWJ07_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9333903.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            6538..8022
                     /locus_tag="IWJ07_002366"
     CDS             6538..8022
                     /locus_tag="IWJ07_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAP9333904.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
BASE COUNT         2517 a         1281 c         1705 g         2547 t
ORIGIN      
        1 ctaaagcatt gtaaaaaaat ggacgtctaa ctatatattc gcttgtatat agtatatctt
       61 gattcgtgga atcatagggt ttctttacca tctttttggt gacagttggt agtttgagat
      121 tccgggactt gaaaaaagct tttttcttat gctctttcaa ttccagctct ttatcgaaat
      181 atttgctgat atagcgcccg cgattttcga tactgtctac atcgatcttg ttgatcttaa
      241 cgaaaccctg accccaaatc gtagttaacc ggttaaatga gataaagggg aaatcaaaca
      301 agataatatg gtagtggatt gcaccgcgtt gctgcttttc ccacgtggcg agatacttta
      361 aatttgcttt tttagtttta tagaggtcat aattcagtcg tttgataaat tttttaaatt
      421 cattatttgc atactcaatg tcttgaatgt tttctttgaa agtgagagtt aaaaatttcg
      481 tttgattgtc aaaattcgtg tctacaaggc gggcaatctc aaatcgcata ctttgataat
      541 gtttctgttt gcgttttaaa ctgtcatatt gtccttgagc ggataattca tcatagtttt
      601 ttcttttttc aagagtaata tcattttcat aaatccaagc taatttttgt tttcgttcca
      661 gatttacttc gttttcgatc gttttacgtt tgggagtaat gattgcttca taatcataaa
      721 tttcaatata cgtgggagtt tcaatgattt tctgattata agcttgcaaa aatcatcact
      781 ccttgttgcg tattcgattg tcaaagaacc agtcattttc tcctgatgtt gatctataaa
      841 tcaagttaag ggggaaggcc tgcgtagcag cccttctagc ctatggggtg ttgggcggtc
      901 ttaacggaca gaaacggctc tgtgggcgct gccccgccgt ttcgtcctaa ccacccaaca
      961 ctccatagta tagttagttt tgtgtagtgg taccttgttt tctgggaatc aaagttgccg
     1021 tatcagcggt tgccgataac ccataactga caaattgtcc attcgagcgt ccccaaggta
     1081 caatcaccaa attggtaaat gtaattatgg ctaaaccttg ttgggtaagt tcttccggcg
     1141 tgatcacagg atgttcagat ttgactttga cactgatttt ttcgtagcga agttttggta
     1201 ggatacattc gtagctccaa cctagaattt cgccctcctt ttgccagcga gtgacattga
     1261 cgacttggta ttctttttcg agtgtttctt ttggtaaact gagatcggta atttttattg
     1321 gcatcacaat tctccttttt tatacgaaag tttatacgat ttgattaaaa aatatagatg
     1381 gccatctacg acttgtatta tatacgagaa cttatacgta gtcaagagat tttctaaaaa
     1441 aatatatacg attttttata cgagtgttat aatgaaaaaa atgataagga ggaatttgaa
     1501 tatgtttggt ttagtacaac ctaataaagt gaaggtgggc caacgaatca aagaaataaa
     1561 agacggaatg tctctctctt ttacagaatt agggaatcga ttaggtttaa aaaagccgac
     1621 gatcagttcc tacgttcaag gctatgcgtt ggctcctaag agtgtgatca accaattatc
     1681 tagtatcagc ggaaaacctg tcggctggtt ttattttggg catgtggaag aatatatccg
     1741 agagtatttg tttctcattg gtcagaaaaa aatagtggac gattatcctg aaatcattga
     1801 gaaaataaag caagaatttt atacgggcga gttcaaaaat cctgcttggg agaatgagct
     1861 aggctatcct ttggaggaat ttatccaaga atgttttgta gaatattcga gtgaaatcat
     1921 gaaggactat gtcgtacaga ttacgaaaga ggtacttgaa gaatccaaaa cgctaaacaa
     1981 actatcatca aaagcaaaag aagaagctgt cacgattctt tcatctggga tcattgacta
     2041 tatcgagatg agtggtgaga tcaagtatgg agagaaagat cgcattgcga aaatggtgaa
     2101 agaatcaata aaaacctttg atgtgaaaca agacctgaca tttaatgatt cctatttgat
     2161 tggaaagttg atcaatattc ttggtacaga tgaagatacg gagctgtttc tttcttcttt
     2221 atcaatgata tttactgata agcatttttc cactcgattt ggtggggaag aattagtcga
     2281 aatatttcaa tcgatgcgac cagcgttaat caaactctat cgggagaaaa caccagatga
     2341 attttatgat tggtttgaga agtaaaataa gaaaagtggc ttagattaca agttggtgaa
     2401 aaaatttttt tcagcataac atcagtaatc tataaagggg ttgttgatta tactgtagaa
     2461 gttaagtact caagagattt tttgtacgta aacaaagcaa aaaagggtgc agtttcacta
     2521 gaaaatgtac tcttttttgt gatttttctt agttatagag tttttttgaa actttaaaaa
     2581 tgagaaaaca tattgatttt aaagagtgta gacatagctt cttttgctaa atagattaaa
     2641 cttactggtg agtggtcttg aacatacttt taaatcacat tttactgagt tttattggat
     2701 tttataattc attggaaacc tcgtacttaa tctgttttta tctatcgact agaatctgta
     2761 tatattccag ctatcaatga ggccgcagaa aagggtgctt aaaaataatc tgttgtttaa
     2821 aatattatta tcagaatact tagatacaaa attctggcac aaagtaaaaa aaatattgac
     2881 ttaaaaaaat ggatatggta atctataaac gaaaatagtg gtaacgtttc caaaatgcat
     2941 cttcgttaaa tttttatttt tttaaacgaa aatggaaacg tttctaaaat gtattgggag
     3001 gagattgaat tggaaaagac attgggacca ggtggtgaga taagggcggt acaacgggat
     3061 aaaaaaaata tttcgaagtg ggaaaaatta aagaaacagt tgcggcgtga taagtggatg
     3121 tacttgttgc tcttaccagg tatcatcatg acgtttatct ttaagtatgt gccaatgtac
     3181 ggtgctgtga ttgcatttaa agactataat cctatgctag gaattttgga aagtccttgg
     3241 gtaggactag atcactttag ggaatttatc agttcaccaa attttacgct tttgctgagc
     3301 aacacattaa aaattagtat ttatggttta ttgttaggtt tttttccacc aattatttta
     3361 gcactgagct tgaatcaaat tatcaataaa aaaataaaac agaaaatcca gttgattcta
     3421 tatgcgccga attttatctc agtagttatt attgtgggga tgctgttttt gttcctatcc
     3481 gcaaatggac ctattaatgg gttagtagaa aaggttacgg ggcaaccaat tatgttcatg
     3541 tctaatccag cttatttcag aacgatttat attttatcgg gaatttggca aggaatcggc
     3601 tggtcatcca ttctttacac cgcaacgctg gcgggagtca gtaccgattt atatgaggca
     3661 gcaaatatcg atggggcaaa tattttacaa aagatttggc atatcgattt gcctgcattg
     3721 aggcctgtga tggtgatcag cttcatcttg gccgccggcg gtattatgaa tgtaggatat
     3781 gaaaaagcct acttgatgca aacttcaatg aacatccctg cctcagaaat catttccact
     3841 tatgtataca aagtcggctt gcaatcaggt gattatgcct actctgctgc tgttggtctg
     3901 ttcaatatga ttattaatgt cgttttatta ctgattgtca acaatataac caagcggcta
     3961 aacgatggtc aaggcttgta atgaaaggag aaaattatga atagtgcttt atcaaaaatc
     4021 gaccgtcaaa tcttatggtt gaatcgagtg attgtaactt ttttagtcgt catcaccatt
     4081 gttccgctga tttatgtact ggtagcttct tttatggacc cagctacttt gatcaataaa
     4141 ggtataagct tcaatccgaa agattggaca atacagggat atcaacgggt atttgcagat
     4201 gattccattt tacgcggttt tgtaaattcg ttgttttact cttttagctt cagcttattg
     4261 actgtattta ttacgatgat cactgcgtat cctttgtctc gacctaactt ggcggggaaa
     4321 aagattatta tgactttttt tgtcattaca atgttcgtgg gtggtggctt gatacctacc
     4381 tatttattgg taaagaactt gggcatgtta gatagtgtat gggcgattat cgtaccaggt
     4441 gctataaatg tgtggaatat tatcttagcc cgtacctatt ttagtgcttt gccaaaagaa
     4501 ctttctgaag ctgctactat cgatggcgcc aatgacttgc aaatactgtt caaagtcatg
     4561 cttccattag cgaaaccaat tatgttcgta ttgtttttat atgcctttgt tgggcaatgg
     4621 aactcatact ttgatgccat gatttacatc aaagatcccg acttacagcc gttgcaactc
     4681 gtattaagaa atattttgat ccaaaaccaa ccttcacaag acatggtggg agccaatact
     4741 gccatggcag aaatgaaaca aattgctgag ttgatcaaat acgcaacgat cgtcatttct
     4801 agtctgccat taatcataat gtatcctttc ttccaaaaat attttgataa aggtgtttta
     4861 gttggttctg ttaaagggta agtattgttc actatttgta tgaataaaaa ttataaagga
     4921 gattttaaga tgaaagcaaa atggatgctg ccaatcgttt ttacttccgt actaattttg
     4981 ggggcttgtg gaggctcaaa agaaaagagt aaatcaagcc ctgattatga actagaaaat
     5041 gtctcttttc cattgaaaga aaaagtatct ttaaaaatga tgtctcaaag tgcccctttg
     5101 gctcccaatg atcctaatga caaattgatt ttccaacgat tagaagaaga gaccaatctc
     5161 catattgatt ggacaaacta cagtagtgat tttgccgaaa aaagaaattt ggatatcgct
     5221 agtggtgatt taccagatgc catcttcaac gccgctgcag gtgactatga tttattaaac
     5281 tgggcggaaa gtggcgttat tgtaccgatt gaagatttga tcgaagatta tatgccaaat
     5341 ctgaagaaaa tttttgatga aaaccccgaa taccgacaaa tgtctactgc acctgatggt
     5401 cacatttact ctatgccttg gattgaagaa ttaggcgaag gaaaagagtc gattcataca
     5461 gtaaatggga tggcttggat caacaaggag tggttggata atttgggtct tgaaatgccg
     5521 caaaccactg atcagttgat gactgtgttg gaggctttca agacgcaaga tccaaatggt
     5581 aatggtgaag cagatgaaat tccttttagc tttattaaca atggaggaaa cgaagactta
     5641 aaatttttaa tgggggcatt cggtctaggt gataatgatg accacttagt cgttgataac
     5701 gatggtcagg tacaatttac tgctaacaaa gaagggtata aagaagccat caaatatttc
     5761 aatgaaatga ataataaagg tcttattgat aaagaggctt tcgaacaaga ttggaatacg
     5821 tatgttgcca aagggaaaga acataagtat ggcgtgtact ttacttggga taaagcaaat
     5881 atcacgggtg cagatgatag ttatgacgtc ttgccagtct tagcgggacc agatggaacg
     5941 aaacatgtta ctcgtacgaa tggcatgggt ttctcaagag atcgtttcgt aattactagt
     6001 gcgaataaaa acttggaact tacagctagg tggatagata aaatgtatga accgttacag
     6061 tctgtccaaa ataactgggg aacctacggt gatgaagcac aggcgaatat ttttgaatta
     6121 aaggatggta tgttaaaaca tttgccttta gaggggacag caccggggga actaagacaa
     6181 aaaaccgaag ctggaggacc attagcagtt cttgattctt attacggcac agtcactact
     6241 atgccagatg atgcgaagtg gcgtttagat ttgatgcatg acacgtattt gccatatatt
     6301 acagaagaca atatctatcc acgtgtcttt ttagaaaaag aagaaacaga tcgattagca
     6361 aaaatcgaaa cggatatgtg ggattacatc tatcgcaaaa gagcagagtg gatcaccggc
     6421 ggtaaaatcg atgaagaatg ggaagaatac cttgctgaat tggaacgtgt aggtttggaa
     6481 gaatggattc aaataaaaca aactggctat gataattttt tgaagggagc acaataagtg
     6541 ttagtagaag aaacggcgat tttggataaa gcaaatcgat ttattgaaat gatgcgagga
     6601 aaagttaacc ctcagtatcg acaaaactat catttttccg cacccatcgg gtggataaat
     6661 gaccccaatg ggtttatata ttacaaaggg cattaccatc tcttctatca atactatcct
     6721 tacgatagtc gatgggggcc catgcattgg gggcatgcgc gcagtagtga tttggtccat
     6781 tgggaagatc taccagtagc gttagcccca gatcaacctt atgacagcgg cgggtgtttc
     6841 tcaggaagtg caattgaaag agacggaaaa ctatatttat tttatacagg ctgtcaagaa
     6901 attgatggta ttagccatca gcatcaatgt ttggcggtct ctgaagatgg tattcatttt
     6961 agcaaataca gtggtaatcc tgtggtagca gaaaaacagc taggtgaaaa tgggctaata
     7021 caagaatttc gtgatcctaa agtaattgtt cacaacgatg gttactatat ggtagtagct
     7081 actaaatcta cacagtctac tggtagaata gtgatgtttc aatcgtcaga tttgtatact
     7141 tggcactttt actctgtgct gctagaaggg acttcagagc aaggaataat gtgggaatgt
     7201 cctgatttat tccatttaga cggacgagat gtcttaatca tgtctccgat acaaatcaag
     7261 aagaacggac tggaatatca taatatcagt tctaccatgg cttgtattgg tgaggtaaac
     7321 tgggaaacag gtcagatgat agttgataat ttccatgaaa tggattttgg aatggatttt
     7381 tatgcaccac aaacaacaag agatcctaat ggtggacggg tgatgattgc ttggatgcag
     7441 atgtgggatc gtacattgcc tacacatgag ttaggccatc tttgggcagg ctctatgagt
     7501 ttgccccgag agttaagagt gaaaaaaaat cgattggtac aaaaacccat ctcaactgta
     7561 tatagtcatt tagtttatca acaaggcagt gaaaatattg cagtgactga cacatcagtc
     7621 gtatttcgaa acgcgctgtc tgattcttca tatttacatc tagtcgcaga tttggagaaa
     7681 gctgaagttt ttacaattga attactgcgg tctaagacgg attgtctcaa actagcctat
     7741 gacactgcta cacaggtttt tgaattggat cgtcagaaac taggaagaga gcttacaggg
     7801 gcggaagaag accctctgaa tgtacgaaaa gttgaagtac ctttgattga taatcagctc
     7861 gttcttgaga tttttagaga tacctcatca attgaagtct ttattaatgg gttagaagtt
     7921 atgacggcta ctttctatga gctggaaaaa gctcgagata tcttgtttca ttcgatcgga
     7981 gaaagtcgta ttcaagcttt tgaaacagcg agtgtgaaat aaatgtttga ttgcttaggc
     8041 gatgaatcaa
//
LOCUS       DACDPS010000096         7743 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.096, whole genome shotgun sequence.
ACCESSION   DACDPS010000096 DACDPS010000000
VERSION     DACDPS010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.096"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            54..533
                     /locus_tag="IWJ07_002367"
     CDS             54..533
                     /locus_tag="IWJ07_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333905.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            566..2794
                     /locus_tag="IWJ07_002368"
     CDS             566..2794
                     /locus_tag="IWJ07_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP9333906.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            2923..4566
                     /locus_tag="IWJ07_002369"
     CDS             2923..4566
                     /locus_tag="IWJ07_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP9333907.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4639..5184)
                     /locus_tag="IWJ07_002370"
     CDS             complement(4639..5184)
                     /locus_tag="IWJ07_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAP9333908.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5207..5692)
                     /locus_tag="IWJ07_002371"
     CDS             complement(5207..5692)
                     /locus_tag="IWJ07_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9333909.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5682..6494)
                     /locus_tag="IWJ07_002372"
     CDS             complement(5682..6494)
                     /locus_tag="IWJ07_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAP9333910.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6633..>7743)
                     /locus_tag="IWJ07_002373"
     CDS             complement(6633..>7743)
                     /locus_tag="IWJ07_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP9333911.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT         2629 a         1478 c         1538 g         2098 t
ORIGIN      
        1 taaactacta atttacttat ttactaaaaa taaaaggatg aggagttaaa ataatggaaa
       61 taccaacaaa tttaaaacat aaaccagtaa tcgttgtaga agactatgat aaagttgatg
      121 gaagaaatgc attaaatacg gatgcaaaag ggctttcact gggccttgct cagtggaatg
      181 atcgtggaaa agtcgatatc tcaggcaagg tatggcgaca tactggagaa aaatggtcac
      241 gacagtcgga agaattacca atcacacgta ttttagattt agcgatctta gctgcacaag
      301 gaagtttata cttccaagat gcttatcgtt atgaaaaatt ttatgatcca gaaaatccag
      361 tcgtagacat cattggatta caagggaacc gaatgacggt agaagtagat accaaaaatc
      421 caaagatcga tgaagacatc attttgtatt acgatacact acaaaaagac ggagaactga
      481 ttggggaacg attccgtatt ttaaaacgtc ttttagatga gctaggatat taatagacag
      541 agatgatcaa aaggggaaga gaagcatgga gaatttctca ctaacagaaa accgaacctt
      601 ttcacggcta gaaaagcaaa tgatctggaa gaaaccgaaa agccatcgga caagtgcaga
      661 agaatggcgg atcgcctccg aaatcaaagg gaattggcaa gatcctgaaa tgaaaatcag
      721 taacgtactt ttggaaggag atgccgggtc ggggaagaca caattagcca aagcactatc
      781 tgctgatctg caacttccct atacaaaagt gacctgtttt gcagatatgg acaaatcaga
      841 tgttttcgga gccttattac ctgtagtaga ttctaataag gaacaagacc aagaattact
      901 agaagctatt taccaaacag atacacttga agctgtactg acactgatcc agaaccatta
      961 cggcattgat cctttttctg ctaaagaaaa attagcagag ctagttcaaa ggatcgaagc
     1021 tgattatcca gatgcaatcc attacagcta ttacccttct gaaatcgttc gagcgattga
     1081 aaaaggatat ttattagaga ttcaagagcc gactgtcatc cgagatgcct ctgttcttgt
     1141 ggcacttaac tcagcgctcg aaccaaatgg gatgttgaat ttaccgacag gaatcattcg
     1201 tcgccatcca gattgcgtca ttgtgatcac tacaaatcga aattaccaag gaaatcgccc
     1261 attaaatgaa tctttaagag atcggatgca acatgcggaa aaaatggatc ttcctgcctt
     1321 agaagtcatg gcagaacgtg cgatggcgaa aactatgatc caaaaacaag aacttctctt
     1381 aaagatggca gagattatcc gtttgttgga tgaaactgca aaagctaacg ccatcaaagg
     1441 tgtagcagga atgcgttctt atttttattg ggcaaatacc tataagcaag gacaagattt
     1501 actccagtcg atttatccaa aagttttgta taaattatcc actgattcag atgaattggc
     1561 aattcttgaa caagctttgt ctgacagtgg attgctactg gaacttcagg acatattgag
     1621 aaaagaaaaa tggggtcatc ctcaatctga gcatacaaaa ggaagaacca ttagcgccga
     1681 agaagcaaat gaacgaaaca tcactgaaaa tgctgaagaa attctgcgct tagatgaaaa
     1741 gaaatcgttg tctgctgaag aaccaaatga gggagaagag aaacaagaag aaacaataga
     1801 aaaaaacgag attcctcctc aaatgcaaaa agaacgcagt agcgatatcg aaggaaaaaa
     1861 tcaaaaagaa caacaaacag gagatgattc tcaagaaatg tctgcttctg acatggaagc
     1921 acatgataag gaaatcaaga agcaactgaa taaagaagcg cgacaaatca tgaaggaaac
     1981 gattcatgaa aaagaaggtc tcatcgttca ccgaccagct ttccaaggaa aaacgccgga
     2041 agcacgtgag ctacacatgc aagtaatgcc aatagtagaa tcacttagcc ggcaaatttt
     2101 agagttactg gataatgaac aaacagagac gtaccaaaaa ggaaaatacg aaggtcagcg
     2161 gttcaatgca agcaaagtag catatggtga tttgagaaat tttgacaaaa agaacccgcc
     2221 acatgagcaa ccttctttag cggtagctgt acggatcgat gaatctgggt caatgatacg
     2281 ggatgatcgg atcgaagcgg caaaaaaagc agcgattgcc attgccgaat tttctaagaa
     2341 agtcgatatc ccattgttga tttacggaga tacagcagac cgttcgtcaa gagaaaagac
     2401 ttctttgttt tcttataaag aatttgaaga cggatttcaa tggttaaatg aaaaattagt
     2461 cactatgaaa ccaaggcaaa acaatcgtga tggtgctgca cttcgtttag cagcagaaaa
     2521 attaaacaga caatccgcaa caaccaaact gttgcttaat attagtgacg gtcagccaaa
     2581 agcgttgccg gattatacag gcaaaaaagc aaaagaagat atccagtctg ttttgaaaga
     2641 atatgaacgt caaggaatcc tatttgtctc cgcagctatt ggccaagaca aagaagaaat
     2701 caagtcgatc tacggtgaaa gtcgttttgt agatattact gacttgaatg aatttcccaa
     2761 gcaaatgatt caactgattg cccgctatct atgataaaat aaagctcaac gaacgcactt
     2821 agcagattcg ttgagctttt cgtttctagt taccggtaac aaggatactt ctaaaataga
     2881 atcggttata atcaagaggg accgttaaag gagtgaaacg atatgataca agaacaaaaa
     2941 tggtggaaag aagcagtagg ctatcaaatc tatcctgcta gtttcaaaga tagcaatggc
     3001 gatggtcaag gagacatcaa tgggatccgt gaaaaactta attatttgaa agatcttggt
     3061 atcggcttta tctggatcaa tccgatctat caatctccat ttgtcgataa tggttatgac
     3121 gtaagtgact atcaagatat agaagagaga ttcggaacga tggaagattt tgatttattg
     3181 ctaaaagaag cacatgaatt agggatcaaa gtgattatgg atcttgtcat caaccatagt
     3241 tcggatcagc atcaatggtt tgaagaatcg aaaaaatcta aagaaagccc gtatcgcgat
     3301 tattacattt gggtcgacgg ggtagatggc aaagaaccga ataattggac atcgattttc
     3361 ggcggttctg cttgggagta ttctcatgaa actggacagt attatctgca tgtttttgca
     3421 aaagaacaac cagacttaaa ttgggaaagc gagaaattga aagaagaact attcaatatg
     3481 atccgatggt ggttggataa agggatagat ggatttcgtt tagatgcgat ttctcatgta
     3541 aaaaaagatg aatattcagt aaaagccaca gagaaccctt tctcaccctt tcaaaatgtc
     3601 tcaggaatcg aagaacattt gaccgatttg aaacatgttt ttgaagagta cgatatcatg
     3661 actgtcggag aagcaagtgg tgtcactgca gaagaaggac cacaatgggt agggaaagac
     3721 ggttatttcg atatgatatt cgaatttgat catatccata tctggcagca agaaaaagaa
     3781 gggcaattag atgtccttaa attgaaactt gcattaagcg cttggcaaac ttcattggat
     3841 ggtatcggat ggaacgcttt atatatggaa aaccatgatg ttcctcgtgc cgtttcagtg
     3901 tttggagata ctcgtcccga tttttgggct atgtctgcaa aagcaatcgc tatgatgtat
     3961 ttctttttgc aaggtacgcc atttatttat caaggacagg aaatcggaat gacgaatatg
     4021 ccctttgaat cgatcgatca agttgatgca gtagattcga aaagacttta taaaaggctg
     4081 cttgcggaag gtaagacaag agaagaagca ttggatatca ttcgtgaaac aacgagagac
     4141 aacagcagga cacccatgca atggacttcg gaacaatatg ctggtttttc tacacatgaa
     4201 ccttggctga tcactaatcc aaataccaaa acgatcaatg tagaacaaca agaatatgaa
     4261 ccagagtcgg tcctgcaatt ctataagaac atgatccgca ttagacaaac caataaaggt
     4321 ctgatatacg gttcttataa agaatatctt catgaacatc cacaacttta tgtctatgaa
     4381 agatatttag aagatgaaca atatcttatt atggtaaact taacagaatc acttgctgac
     4441 tatgaacttc caaaagaagc agatcagtcc tggacattac tcttgtcaaa ttcctctagc
     4501 ggagaatttg aagctaaagg catactagca ccttatgaag ctcggttata taaaacaaac
     4561 aagtaaaact tcctcgttaa agttcattag ttggactttg cgaggaagtt tttcagattg
     4621 ctctgtcaat tactcatttc aataattcgc tctaggacca ccgatatatt taggacgaga
     4681 gcgaagggct gtcacgtgag cttgtccaag ggttttcaaa cgaggtctga caggtacttg
     4741 gacatgtttg atatgcatac cgattgatgt gtcaccgata tcgataccgg cttgtccaac
     4801 tacgtgttcc acttcgacag gatcattgaa ctggtcaaaa gcagcaacag aacaagcacc
     4861 tccagcatgt agtgcaggaa caacagagac aatttcgaaa ttttttgctt ctgccactgt
     4921 tcgttctact actaatgctc ggttcaaatg ttcacaacct tggacagcta gcgcaatttc
     4981 tttaggatcc aacaactctt ttaacgtacg gatgatccat tgaccaactt ccgcactgga
     5041 attttttcca atatgtcctc caacgacttc gcttgtgctg catcctaaaa caaaaagatc
     5101 accttttttc aagtgtgcct cagccaatat gtcattaact atttcagtca attggtcttt
     5161 taattctttt tctgacaact tcattttcta aaaccttctt tttcaattaa tattgcttcc
     5221 gtgcttgaat acgttgaaga cttgcagtta taggaaaggc aagtcccatt ccaatcagat
     5281 tttggaagat gtttcctggt atagaagcca ctccagccgg ccaatcatat aaaatccaac
     5341 cggcgatacc atagccgatg atcataacta agcttcctaa taaaagccct aatccccaca
     5401 tacgagtaga agatttgctg aaagaaccag caacatatcc ttgtaacccg tggatcacta
     5461 atgaaaaaac tgcccactgc ggatatcctg aaatcaaatc gatcaataga ccactggaag
     5521 caccaacaac taatccaccc gttggaccat aaagaaaagc agcggtatag atcccggcct
     5581 cgcataatgt cacaaatcca ttcgtagccg gtacagggat caaaattgtt aatgaaaggg
     5641 caactgtcaa cgcaatcagc atagcataga tcgttaattg cttagttttc atttctcatt
     5701 cctccttttt gaataccgcc agaccagaca cttccggtat gatcgttcat cagcacgcca
     5761 ttagaaatac aatgggcaac aaatttttta ctttctgcta ctgctttggt aaaatctgcc
     5821 cctaacacaa gattcgctgt tattgcagaa gaaaaggcac aaccggcccc gtttactgtt
     5881 gtagtgtcga tttttttatg agaaaatacc tcataatgat caattgaata gagaagatcc
     5941 acagcttcat ctccagccat ccttgtgccg cctttgatca caatgttttt tgttcctgaa
     6001 gcagtgagtt ctttagcgag atggatcatt tctgttaacg aagagggaag agcttgacca
     6061 gaaagtaaag cagcttctgc caaattaggc gtaatcatcg taacaagtgg aaaaagttct
     6121 tcgatgagct gcgaaatata caattgattg atcacagctt cagtttcttt gaatgccaaa
     6181 acagggtcta aaatgattgg gatattttgt tttgttttta tgaagtctcg aacaaccatg
     6241 attgcttcac ttgaattcaa taacccaatt ttgataccat ctaatgaagt cattttatca
     6301 atcgaaacta actgtcgttt aagcaattcc aaagatatag attccagcat aaatcgctca
     6361 ttaatatcta gtgcccctat agaagtcaag gcgcttagtc cgaatagacc aaaattctca
     6421 aaagttttta aatctgtttg gattcctcca ccagcaaatg gatcagaacc gccaattgtc
     6481 aagattcttt tcataacatt ttcctccatg aataagagta taacgaaatt ttcaaaaaag
     6541 aaaaacagcc aattggcata tttttatctc atccaattta aaaaaacaaa aagaaaagaa
     6601 agaccaaaat ggatctttct ttccttaaat agttaggact tttgtgattt taattctttc
     6661 tcgatttgaa gccgttgcac tttcattgta gctgttcgcg ggattgcatc aaaatcacgg
     6721 atgataggga cgttcaagcg aggaagttca tggacttgct cccaccatcg attccaatcc
     6781 atttctttat caggagctaa agcaataatc ggttgtggag aatgattttt atcccttaca
     6841 atcactactt ctgccaaaaa atctaacgaa tctaataaaa agtcttcaat agctaatgta
     6901 ctattgatcg tttcgatcaa atccacttgc cgatctttca aaaataggtg gccatgctta
     6961 tccataaaac cgtaatctcc gctatcccac caatcgccat aaacattttc ctggaaccgt
     7021 gcatcttcct tgtaataagt caaagcccgt cctttagaca gtaattgtat atgaccatct
     7081 gtgttttcag gtaggacatt cccaactgaa tctgtaatcc gagcgcttgt catgtcttcc
     7141 agtccaacgc ccatatctct tgcatcactt gttttcaaac tctctagtgt atgagcctta
     7201 agaatcattg gtccgcattc actttggcca tatacttgaa gaaataccgc atcctgtgcc
     7261 atcgacgttc gcaaaaatgt cttcattgtc tggttgttta tagcatcaaa agtggagtga
     7321 tagtatcgga tacccgaaaa agcttctgga tgctccttag cagtaaaacg ccactgaaca
     7381 aagttattcg gatgggtctc tagtgcaatt ggccggtgag cttccaacat atgaacaact
     7441 tttgaacttt gtgcgtttgc taaaggcatc ataggaaatc ccatggacat caaagaagag
     7501 ataccaatat tgaatctgga atgcactgga gagatgtgaa aagcgattaa tttcttttcc
     7561 gaaatttttg tgaaaactgt tttttgccat ttcgtccgcc atcccataga atgagcagaa
     7621 tgacaaataa gtttagggat accagttgta ccagaagtat gggtcatata agaaatttca
     7681 tttttgtcta aaaatgattg agaaacagac aaagcaggtt gttccattaa ataaagaata
     7741 gga
//
LOCUS       DACDPS010000097         7627 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.097, whole genome shotgun sequence.
ACCESSION   DACDPS010000097 DACDPS010000000
VERSION     DACDPS010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.097"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(16..708)
                     /locus_tag="IWJ07_002374"
     CDS             complement(16..708)
                     /locus_tag="IWJ07_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9333912.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEIGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            793..969
                     /locus_tag="IWJ07_002375"
                     /pseudo
     CDS             793..969
                     /locus_tag="IWJ07_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1145..1714)
                     /locus_tag="IWJ07_002376"
     CDS             complement(1145..1714)
                     /locus_tag="IWJ07_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP9333913.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1788..2111)
                     /locus_tag="IWJ07_002377"
     CDS             complement(1788..2111)
                     /locus_tag="IWJ07_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333914.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2265..3077)
                     /locus_tag="IWJ07_002378"
     CDS             complement(2265..3077)
                     /locus_tag="IWJ07_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333915.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3431..4279
                     /locus_tag="IWJ07_002379"
     CDS             3431..4279
                     /locus_tag="IWJ07_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAP9333916.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4424..5365
                     /locus_tag="IWJ07_002380"
     CDS             4424..5365
                     /locus_tag="IWJ07_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAP9333917.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKTFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5355..7526
                     /locus_tag="IWJ07_002381"
                     /pseudo
     CDS             5355..7526
                     /locus_tag="IWJ07_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2273 a         1512 c         1372 g         2470 t
ORIGIN      
        1 agaggggggg attttttatc gttgtggaat atttaaatac caacgcttgt catgaaaatc
       61 ttgtgctcca cccttagtat ttccttctgg atcgtttgtc gcacgcatca tgacgtatac
      121 tttcttatta gggaaatcac gcatattgaa agatacatga tagccaacat ttcctaaagt
      181 attataagct tgatttacgt ctggtctata aatcccatca gctcttactc tagctagttc
      241 ttttccagta ttgtagtcca taatgaaaat atactcgtat ttataattag caatgtgcca
      301 tccagctaca tgcaagtttg cgttttcgat ttctccgaac tgatcaatgt gggcgtaatt
      361 tgttccatct gtcagcgtag aatttgcagc acctgctcga gttggatcaa ttactggttt
      421 atcctccgaa gtagttggat tatcatccgt aaaaccatga gccaaatcat aggctagttt
      481 ttctttactt acacctattt cagaaagata accgtaagga tctgtatgat cgccccaaat
      541 attttgtgtt acccataaat gcgatttgat tcctggttgg ttataaggag tgtctaatgt
      601 taatggaata ccatatttca ttgctgaatc tcttgccaat tcaacgtata ctttatagtt
      661 cttttcaaaa gtttctttat catgtgtgtg ttgtaactcg atttgcacag gacttttggc
      721 tcacgaggtt gtcctactct acatcttgct gatagatatg ctgaaaggaa aacaccaaca
      781 ttatgttttg ctttttcagc tagaacgaga caagcaggaa tacctttaga agatatcaaa
      841 ggttttttag gtcataaaga tgtttccact acacaagtct atgcccatat ttctcccgaa
      901 gtgaaaaaaa gatcaatgaa tcaactagaa aactatatag aagagcaaat aaaaaagcac
      961 tcaaattgag tgctttttta agctcactat cactaaaact atcactaatt ttatttataa
     1021 gcgaacatgt ttctatcact gattttttaa atctagcgaa aaacccttat cttaaaggcg
     1081 ttcgttcaat tctttggcaa ggtcttcaaa gcctggcttg cctagaagtg cgaacatggt
     1141 tttcttatag ctcatgtaga aaaattttct tgatgatgat tttatccatc aattgagatt
     1201 gactgttttg ctctcctatg atttcagcta tgattgtccg attttgcggt tttcttctta
     1261 actggtagtt gattttccca gtttccatca actcgctaag catatccacg actccgccaa
     1321 atccggtctt tttcgtaata aaacctagtg ctaccattcg ttcatctaga ttttttgctg
     1381 taatcgtcac accagtaatg agcgagtgct gtaatgtttg ggccagctct tccatccctt
     1441 cagcattgga tacgacggaa gctgttttgt taataggttc ttcgtttatt tcttttggtt
     1501 ctttcctctc cactttcact gtttgacgaa ggacatctag ttcttgcaag agcgtttcgt
     1561 attgttcttg ggtcagcatc acgccagcta ctttttcacg attaaatata tatacgccag
     1621 ttcccgcgtc tcgcgcttga gcaaatacat ccatcggcga tcgtttcact tctgtgatcg
     1681 aagatgtggg aacttctaat ttctttaatt ccaatggatt tcctccttta ctaatcgctt
     1741 tttattatag cacagactga cttgttttga tcttttttcc aaaggtttta tcggtttttg
     1801 cctaaccagt aattgatatc ggtaagaagt ctttcgatac caaatgaagg atggtacaga
     1861 tacgtttttt gatcttcgtt atggtctagt ggaaaaaaat gatcgatgat cactagatct
     1921 gcataccgag gcggcttatt tcgagtacat acatgtatag agatccgttt tcgatgtctt
     1981 agcgttttgt tcagtacccc agcaagtctt cttgcttctt ggtgttcata gcgaaaaaaa
     2041 aagacgattt tcacttcagg agggtggaca tctaaatacc ctcgttcctg gagtgttttc
     2101 agtaaaagca tcccccactc tactgcgggg tgtgcaagtg cttgttggat cgctagccga
     2161 aattgatata gtttatcttt ggaattggga aatttttcta atgtttcctg ataataacga
     2221 acttcttcta tcagccggtc atggatacca attacgtaac gaagtcattc ttggtaaagt
     2281 acttttgtca gctttgattt gctttcgctc tcaaattcgc tattcagcca aaacagcgaa
     2341 tcaagctttt cagtcacaat cgtcaatttc cgatagtctt gtgtctcttc tatccgtttc
     2401 caccgctttt cagtaatctg gttacagctc aagcgcaaaa ttgctagata atttttttct
     2461 tttattggca aaatctgctc ctctctaaat agcaattcag gaaacaattc aaagaatagt
     2521 gttcgactgt ctcctaaaat ctcgatttct gccggatcct ttctttgctt caataattgg
     2581 atgaacatct cagaaaagga ctgctctcca tcatctgcaa aaaaatgcgg aaaatctttc
     2641 aaaaagcaaa acagcagata tcttactttt ctttctgaac caaagatctg tgctttttta
     2701 taagaaaaag aaaggtggta ctcgccaaac cactttctca atcgctcttg cgtagaacga
     2761 tcgtacttct gctcgtaagg ttcattcaat agcagatttt tcagtaaaca aaacgggggt
     2821 gattggatca gaaaaacaaa caagagataa ggttcgctat atccttgatc ataaactaac
     2881 tgaacggttt tcttatgaag aatcagctga atgggacagt cgctttccgc caaacgtttc
     2941 agatgccgat ccactgtttt aacatcgatt ttcagttctt cagctaaatc agcttttcgc
     3001 cagatcaaat ctttcattag attgattagt gaaaataaat tctgtgcatt ttttgataat
     3061 agtttttgcc atctcatcga tcatttctca cctgatttct aaacatattg tttgatcaaa
     3121 atgggttcac tcctaaaaag ataaaaaact ccctttctat ttatttaaat acgcaaatct
     3181 tcctcattat tttttatttt actcaattta atcgtattct catttcaaaa cgaatgtact
     3241 ttaaaaaaac ataataagat cactttgctt gtaggatgta ttcccttcca ttacaatgaa
     3301 ataaatagcg attttatgag aaggtgatgt gaaaatggaa gaaaaaatag aagaatccaa
     3361 acaaaaaatc gatcagttga tataagaatt agaaaaagcg gtcatcggca aacgttcaac
     3421 tatccaattg atgatcgtgg ccctattagc aggtggtcat gtcttattcg aagatattcc
     3481 cggtgtaggt aaaacgttac tggtcaaagc actagcaaaa gctgtacagg gaaccttcag
     3541 cagagtccaa tgtactcctg atctgctgcc tagtgatatt cttggttttt ccgtatacaa
     3601 ttcacaatca aaagagtttg aatttcgtcc gggaccgatc tttaccacga tcttgttagc
     3661 agatgagatc aatcgtacga ctcctcgtac tcagtcagca cttttagaag caatggccga
     3721 gaatcatgca acgatcgaca acaatacata ccctctagac aatcattttt ttgtcttagc
     3781 tacgcaaaat ccaatcgagt acgaaggaac atatcctttg ccggaagcac agctcgatcg
     3841 ttttttattc cgactacaaa tcggttatcc ttcttttgat gacgaacttt tgctactttt
     3901 ggataaattc gaaaaagtgt tggataactt aaatgaagta ttgaacagtt atgaaataga
     3961 agaaataaaa acaaatgtgg cgaatgtctt tgtcagtccc gaagtggcat cttatgcctt
     4021 gcagttagtg tcagcaacaa gaagtcatgc tgccatccag cttggcatca gtccacgagg
     4081 aagcctttcc tttattcagg cagcaaaagc ttatgcttta atccatggga gaaattacgt
     4141 cacgcccaaa gatttgcagg atttagttcc ttacgtgttt tcccatcgcc tgatttttta
     4201 cgatcgttca ttgaaggaag acgaaaagaa acaaatcatt catactcttg ttgcacaaat
     4261 tcctattcct gtgaggtaga ttgaggtaga caatggaaaa aatcaaagat tatttcatta
     4321 attttgtgtt atttctggta tatttcatca ttgtgctttt tgctcttgta ttcaacaatt
     4381 ctttaggctg gttcttgttg gcctttttta ctctttatgt atcatggagc ttgatttcat
     4441 taagcagctt tctaaagaaa ctttcggtta cttgtacatt gcagccactc gtttatcgca
     4501 aaaaaagtat gcaggttatt ttttccatag caaaaaagag aactttttct ttacctctcc
     4561 ctcgtttaca aatcggcttc ccagcaatct ttgcagaaaa aaacaagaga ttttgattct
     4621 tacaaataaa ccgcaagaag tccaattgct ttggcagcca aaagaaagag gatgccatga
     4681 aacattaact gtcattttta cagcctatga tagtctagga cttttcagaa aaagtgcaaa
     4741 aagagagatc tcttttcctg tgactgttct gcctgctttt tgtaagattc aagcagaaaa
     4801 attacagaga gttctggaaa aaaaacagca gctgaacgcg taccaaagag gtcttgattt
     4861 tcgggaacat cgcgcttatc gtccaggtga ttctttcaac cgaatcgatt ggaaattgtc
     4921 tgctcatact caagagtggt tataccgtga atacgattat caagaagaag aatgttctcc
     4981 tcttttttgc ttttggggtt cgcccagtcc aaaatttgaa cctctgctag acttgtattt
     5041 ttctttatgg catttgagaa aagaaaagca gccggaactt ctgatattag gcaaccgcgc
     5101 ctttcatggc aaagaccccg gtcataccta ttttgcttct ttggaagctg gcgatgagaa
     5161 aacttttttc gcacacttga aaaaatatgc aaaaaaacaa atcgtcctct ttgttcctga
     5221 gaattctcct gaagtctcag aagctattga aacattgtca aaagatcgaa cggtttatct
     5281 tgtttatttc gtagaaaaac aactcatggt ccaagaaaaa gacaagatat gttcattgcc
     5341 aggaggtgaa tggattgacg attaaacggg aaaaatggct gcgtggcgtt tactcctttg
     5401 tgatactaag tgtaggtgct ccgatatttg tagtagctaa tcaattgcaa aacagctacc
     5461 ttcttatcct aactattggt gtcctatgcc ttattgtctg ctgtattcct aaaaaatggg
     5521 tacgtttgct gctttctttg ccagtaatga taggttgttt gtttttatat tttccttcga
     5581 agacattttc catattatgg atcttacagt ttttttcgtc agttacagag gaattcttcc
     5641 atctatccca aggtaaactt tattatttgc cagaaaagac agcttttttt ctcataatgt
     5701 tgctgatcta tttatttatt acattgaccg tggattacga ctattggtac gcaccctttc
     5761 tttctttgat ggcttacttc atgatgctta ccgtttttcg gaaaaaagat ttactgtacg
     5821 aaatgattgc ggtagtaact gttttgttcg tgtatctggc agcaaggcaa tttttctcca
     5881 tccggttgtt gtcgagaaac actcgattcc agtcattgac tacagttctg cttatttgtt
     5941 tcttaacttt tagcgctatc ttgcctttat atcttccgaa agtacaccaa tctctcgagg
     6001 aaacgagtga accgatccga gaatatctta atgatgaagg gctttatgac acactccatg
     6061 agtatcaatt aaccggaagt gcaagaacag gcttcagtga gaatgatgaa cagcttggtg
     6121 gacctttata tgataatggc gaagttgttt ttacagcgaa ccaaaaagga aatggctatt
     6181 ggaagatcga aaataaaaat cattacaccg gttctggatg aatagaagat caggacgcac
     6241 ccatccaacc aatccaagaa ttccctgttg acatcacgag ctaccaaacc gttatagaag
     6301 acaaagaaaa cattacattg accctaaaaa aacaagcttt cttaccttat ccttatggaa
     6361 atactacctt gactgatttt tcattagcta gtggacaaac gctttactat ttgcctgaaa
     6421 gccagcgatt cattcctgtc ccgaatgaat cgacacgttc tgcaacttta caaacagaaa
     6481 ggttagtttt tacgagagaa gaattaaaaa gcagtcagtt aaaagctgat gatttagaaa
     6541 atcagcagac aaatcttcaa ttgccaaaaa gtttgcctaa acgtattgag gacttggcgg
     6601 tcgaattgac caaaaatgaa gcaactttaa ttcgtaaagt cgaagctgtc taacagtatt
     6661 taaaaacaag cggaaatttc cgctattcca aaacggatac cgggcatact ccaaaagaac
     6721 acgattatgt ggattatttc ctctttgatt caaaagtcgg ctattgtgac aacttttcaa
     6781 cttctatggt cgtccttctt cgaacacttg gtatcccagc tcgctggaca aaaggtttta
     6841 caaatgggct cagaacaggc acagataatc aagggaatga ggaatacagc attctcaata
     6901 gtcatgctca ttcttggcca gaggtatact ttcctggatt tggctggctt ccatttgaac
     6961 caaccccttc atttgccaat ccaatgggtt cacaaccagt tgctgaaaca accgaaaccg
     7021 tccagcctgc tatcgaacaa ccaaatgaaa gcgaaaatat ggcagaaact atttccagtg
     7081 agagatctga ggtcattcca tcagaagaaa caacactcac accagcttat aaaatcgtct
     7141 tattcagcct tggagtgtgt ttatccgtct tgttgttctt tctattgtta ttcagaaaat
     7201 ctctgcgctg gacaagttat cttatctatc ttcatttttt tgcttctact aatacgattg
     7261 catatcaaaa acttcttaac cctttggaaa cattggtacc gcgcaaaccc gcagaatctt
     7321 tgcaggaata tagcctgcga atagaacaag aattcaagct agatctgcgt ttcattcgat
     7381 taactactca atatgaagca gaaatatatg gacgacaagc agatccggta aaattcgata
     7441 aaaaaaccta tttagaaata acgcatataa taagaaaaac ggtatacaaa aaaattctga
     7501 caaaagggag ccgcttcaaa aaataagata aaatttctgg agaatctact tctcgaatgc
     7561 cgagaaccag ctgattgagt atcgattact cgaaaaaaag gttgtgacac gttttttgtc
     7621 acaacct
//
LOCUS       DACDPS010000098         7561 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.098, whole genome shotgun sequence.
ACCESSION   DACDPS010000098 DACDPS010000000
VERSION     DACDPS010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.098"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..132)
                     /locus_tag="IWJ07_002382"
     CDS             complement(<1..132)
                     /locus_tag="IWJ07_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9333918.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     "
     gene            complement(173..427)
                     /locus_tag="IWJ07_002383"
     CDS             complement(173..427)
                     /locus_tag="IWJ07_002383"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9333919.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQKN"
     gene            complement(538..792)
                     /locus_tag="IWJ07_002384"
     CDS             complement(538..792)
                     /locus_tag="IWJ07_002384"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9333920.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(985..1260)
                     /locus_tag="IWJ07_002385"
     CDS             complement(985..1260)
                     /locus_tag="IWJ07_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAP9333921.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1232..2185)
                     /locus_tag="IWJ07_002386"
     CDS             complement(1232..2185)
                     /locus_tag="IWJ07_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP9333922.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2797..4290
                     /locus_tag="IWJ07_002387"
     CDS             2797..4290
                     /locus_tag="IWJ07_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAP9333923.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4425..4721
                     /locus_tag="IWJ07_002388"
     CDS             4425..4721
                     /locus_tag="IWJ07_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP9333924.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            complement(4745..5704)
                     /locus_tag="IWJ07_002389"
     CDS             complement(4745..5704)
                     /locus_tag="IWJ07_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9333925.1"
                     /translation="MTYTHLTTDELVMIESYFKINQSVAKTAHFLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIR
                     CSARTIYRMFKKGLFNPSDLPMKGKRKPNGHQERRGKQAFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIENKLNHWFESVPKNLFK
                     SITFDCGKEFSNWKQISNANDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDESFIQSVASKRNNIPRKSLNYRTPLEVFLSYVSIDDLSNLI"
     gene            5887..6738
                     /locus_tag="IWJ07_002390"
     CDS             5887..6738
                     /locus_tag="IWJ07_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333926.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            6759..6971
                     /locus_tag="IWJ07_002391"
     CDS             6759..6971
                     /locus_tag="IWJ07_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333927.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
     gene            7404..>7561
                     /locus_tag="IWJ07_002392"
     CDS             7404..>7561
                     /locus_tag="IWJ07_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP9333928.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQ"
BASE COUNT         2491 a         1224 c         1371 g         2475 t
ORIGIN      
        1 ccatttgtca aatgctgaag ccgttagttc atattctcga atgatttcta cacgtggctt
       61 tccagctaag taaagattga cgatttgttg cttgaattct tgtgaaaaag ttcttcgtgt
      121 tctcttagac ataaaaattc ctcctggtat gttttcttct agtctacaca ccttaatttt
      181 tctgtctagt taattgtagc ctatccatga tcggtttatt agacctttga ggtctatatt
      241 ttttaactac aatcgacctt aaatttaatt gtttcattag cttttggaca tgtttaagag
      301 atactgaaat cccttctttt aataaaactt gatggatctt tgtggcacca tagcgttgtt
      361 tattcgtaaa aaaaattgtt gaaatcttct ttttcaactt attatttctg atttctgtag
      421 ctgtcaacgg tttattctta taaaaataat agacacttct agggatttct aataaccgac
      481 acaacaaaga aacagtgtaa tccttacacc atttttcaat aaattgaaca agcgaattta
      541 ggttttccga gagaatatgg ctgcggcttt ttttaagatt tcaagttcct catttaaacg
      601 agcattttct tttctcagtt cagctacttc ttttccagtc agtcctgtgg actgatcagg
      661 taaatataaa ttcttccatt tgtaaatcgt tgcttcagat aagccatatt ctttcgctaa
      721 ccctcgaaca gaacgtccag tttgattcag ttcaacaatc atttgtttaa aattttcttc
      781 atatcgggtc ataaaaattc atcctctcgt agataagttt aatttattct tgtctctacg
      841 aaaagctgtc taagatttga tactaacacc aaatgttgta tacccccaaa caatacacat
      901 attttgaagg attaggatta ataaagctaa aaatatagta agtatactaa atatatttac
      961 tatatctagt atacttacta tattttaatc ttttgatcgt ctattatata taatccgttc
     1021 ctcgtcgttt aaagtatcaa cataggcgtc cattgcaagt ttgactattt catacatagg
     1081 aatatctttt gcataactaa gatcccgaat ggtttttagt acgggaggat ctacttgaac
     1141 tggttttctt tctttcgcag tgtactttcg ttcccctaac tcgacattaa tatcatcttt
     1201 aattgaaata tcttcagcac caattttctt cttatctttg ttcgttggta ataatgccat
     1261 ttagtttaat ctcctttcca agaggtagga tatgattggc cattgagtca gcatatttag
     1321 gtgtataatc aaatccttca atatcaccat gttgttcata gtattcaatt ctttcttcta
     1381 attctgtaaa gatgtctgca aaaatagccc accatcttcg atctacataa tcatttaatt
     1441 ggataccatt ctcaccatac atttctaacc gatccgagcc tttaacaatg cttttaaaaa
     1501 tgtttgattc accatactta tcaattgttt tttgatagtt tgcgtgttga gaggaacgtc
     1561 gcactgagaa aagaacaggc aaaattccaa tgatttgaag atgagactct tcaaaattcg
     1621 tgacaatcgg aactaaaact ttattaataa aatcttctgt tccttccatt gcgtaccgtt
     1681 ttaattcttg cattgcaata atataatctg cagcagttaa gaatgcacga accacattat
     1741 ctgttgatgg tgggcagtcg ataaagatat aatcgaaatt ttttctcaat tcttctaaag
     1801 gtttaatgaa ggccaatgat ctcttacgac gatcaggata tttttcggac aagtattcat
     1861 taagttcttc agatccagga gagccagcca taatatataa atgtggactt acttgagtaa
     1921 tacctgaaac ccagtcccct tttttgattg ctgcagacat ggatcgatca aagctagtaa
     1981 tttgatacgt tgatccaatg ttggaagtag tatttctttg ttggtcataa tcaattagca
     2041 acacgtcttt ttcaaaaagt tgactagaaa caatcgctaa taaatcattg tctgtagtct
     2101 taccaatgcc gccttttcta cctgtaagca ttacaacttt tcctagttta tctgatgttt
     2161 taaatgcagt ccgtaagttt tccatagttc cctccaattt cctatactaa atatatttag
     2221 tatacttcat atacttaata tattcgatat atctaatata ctccaaattg ataagaattc
     2281 agactttata aggaatatag tggatatact aaatatattt gctatattca gtatatcaga
     2341 tatattcaaa atgtcaaaat atatttgtgc gttttgcgtt gacaatttag agtgaaaaga
     2401 ggtaaactta attctataga aaccacagat aattcactat ttacaaagta gcaaaagcag
     2461 tggtactctt taaacagaaa aattacataa aaaaaagaga cggaaataat tgattttagc
     2521 agaatcaatt atttctgaat cacatggtta ggacatgtgt tcacgctcgt ctcaagaata
     2581 tgtaagtagc taccaactac ttatattaaa agttgatcgg ataccttttc cgattgcctt
     2641 tattctacca aaaaaaggcg agcaactcaa cagaagaacg tttatttagt gtacttaaat
     2701 taaatattct taaacactga agagagaaca catactatgg ctgagattaa agctaaactg
     2761 tgttctcttt ttgtttgaga aaggacgtta caatcgatgt tgagcgaaat ttattcagct
     2821 attcttaaaa acggcttacg tacatacaaa tatcgaaaca gcaccatgaa acctgcaggt
     2881 catattgatt ccagtaggag tggtgctatc tttgcgtacc gtagcaagca attaatgaat
     2941 gttgggcgtg gtatggtcat tacttcggaa gaagctattt tagagaacga aaagaaacta
     3001 acgcattgga cacctaatac ctatcgcttt ggtacttatg cggatgatta tcgaacagtg
     3061 gttaaaggtc attctgagaa aaacttaagt caaatcaaca catttgtagt tgatatcgat
     3121 tcaaaagaga accatcaagg cgaaattatt ttagcctgtt tagatcaagt gggctacatg
     3181 ccaacgctaa ttttagaatc agatcatggt tatcaagttt attttgtact aaagacacca
     3241 ggctatgtca caaagaaaag caatttcaag gtgatcgatg tagcaaaaaa aatttcaaaa
     3301 acaattcgtc tacagttggc tgaaaaattg tccggagtgg atctaggatg taaccacttc
     3361 ggaattgctc gattcccaca agaaaataat attgttttta aagaaatgaa ctatcagtat
     3421 tcttttgctg actggattca atggtcgatg aagcttgaat cagatcaaaa aacaaaagaa
     3481 gatcgagaac gaaaactaat tgtctttcct gagaaaaaag aaattcgtca agtagatgaa
     3541 ccgtggttcg atatgttact tcataaagca aacatcgtcg gcggagaagg acgtcttggt
     3601 cgaaataatg tgattttcac gctttcttta gcttattact cttcaggtta tgcattggat
     3661 acttgcgaat acaatatgtt cacattcaac gaacggttac aggaaccgct atctgaagca
     3721 gaattagtaa agattgtacg tagtgcttat tctggtaatt atcaagcagc aagcagagaa
     3781 ttcattattg aattgtgcca gcaatgggtt tcaccaacct taaaaaataa tgatctgttc
     3841 attcaacgac gtggctggtg gaaatttaaa aagccacgag atcaaagaaa atatagtcat
     3901 aaaaatgaat ggaaagaaga tctattggct tatctaagcg aaaaaagtct tgctagtgag
     3961 ccttatttga acctgtcaaa aaaagaattg gcttcacaac taacgatgcc caagcgaaca
     4021 cttgataaga tcttaaatga gttacgagag gagaataaag tcttctacaa ggtaacacga
     4081 ggaagaaatg gcggaatgat cattgcttca gtcaaaacgt tgttcgcgag ggttattcaa
     4141 ctaagtaaag aaaagagact agattattca acaaggatca cagaggtatt tggactcaac
     4201 gagagcttag tagaagagac atttagacaa ttatcaaccc ccttgaaccc tcttaaagaa
     4261 gtgaatcttt ttgaagtaga tacaggttga aaaataaaag gtgcaaattg ccttatacaa
     4321 tatatctatg atacttctgt aggtgttgct ccctttgcct agctacgtgt aggtggtata
     4381 cttataactg cgtatgcagt cttatgtatc atggaggaag ttgtatgtcg ttaaaaaatt
     4441 atgtctattc acatcctgtt aatgtgttta tcattggtcg tttgggatta tctgtggaag
     4501 tgttctgtga gctttatggt tttaagcagg ggactttgtc ttcttgggtt accagggaaa
     4561 aaacagttgc ttctttgccg attgagtttc ttcatgggtt gtccttagct tctggggaga
     4621 ctatggatca ggtatatgat tgtttgtgtg tgttagagca ggagtatatt gagtataaaa
     4681 tagctaacga gttaagaaaa aggaagaagt atatccagta gtagttttta tttactctta
     4741 attgtcaaat taagttagac aaatcatcaa tacttacgta actcaaaaat acttccaaag
     4801 gtgttcgata attcagtgat tttctaggaa tattatttcg tttcgatgcg acagattgaa
     4861 taaaagattc atctactgaa ttgaaatcca tagatttcaa taaaccatct ctacgtaaca
     4921 atccgttaga attctcgttt aggcctcttt gagacggcgt tcctggatca gcgaaataaa
     4981 tggcaatatc attggcatta ctgatctgtt tccaatttga aaattccttt ccacaatcaa
     5041 aagtgatgga tttgaataga tttttcggta cagattcaaa ccaatgattt aatttgtttt
     5101 caatatcaat cgcttgtcta ccacacggtt tcaatgtgat gataactttt gataatcgct
     5161 caactaaggt aattacagca cttttatgtt tcaaacctac gatagtgtca ccttcaaggt
     5221 gaccaaactc atttgagaat tggctataat ctttttcacg ttcatgaata gagcgacgga
     5281 aagcttgttt tccacgtctt tcttgatgtc catttggttt acgcttgcct ttcatcggta
     5341 agtcagaagg attaaatagc ccctttttga acatacgata aatggtacga gcagaacaac
     5401 gaataggaaa cgctgcacga ccaacaatca catcgggtgt ccatccttga acaacctttc
     5461 tttgaatata ttctgattgt tcctcaggta aaacaagcgg gcgtctacca cagtttgatt
     5521 tgtttttctt atactgttga tagtactcta aggctgattt tccttgcttg aagaacaggt
     5581 atactttatg gatcgtttgt cttgaacgat tcaagaaatg cgcagttttc gcaacagatt
     5641 gatttatttt gaaataagac tctatcatta caagttcatc cgttgtaaga tgggtatagg
     5701 tcatttgtac tcactcctta taattctttg gacggaacta ttttgagtgt agcacaaatg
     5761 acttttttag ttgtctagct taattttaca atcggcgtac ttaaaaaatt ggttcattga
     5821 gcaacctatc gctgctaaag tagcaaagga aaacgttaaa ggaaagaatt tagggaagaa
     5881 atcgaaatga aatcggacga ctggattgaa cacacctggc ttcctctgaa aagaaatagc
     5941 aaacgaaaga aattttgggt attgcaatta tggccattag gaaaactttg tagttacggg
     6001 gcacgactta ttcttgattt aaccgtttac tcgattggag tatttggaac ggcttattac
     6061 ttgataaatg atcccaatag gattgcagca tcagacattt ttacagtgct aatgacgttg
     6121 atttttgtca tgctattgtc tatttttact caattagtca tgcaatttac caggcaaaaa
     6181 ttaacagcag gaaaactttt caaaagccta ttcgatttaa ttgttgtaaa tgcaattcta
     6241 gcgactttaa taaaacaaat tgaacaatct ggaagaacaa caaaaggagt gaaggatttt
     6301 ttattgacgt ttattcactt tgtaaatgga cacatgtatt tgtttgaagt gggtattatt
     6361 ctcttttttg tcgggtatgt atccacgaaa cttattaaac gttcaattga aaaagaaatt
     6421 gaaattgacc tgattccgac acttcttggt tatgagtaca atgttgattt tggaagatat
     6481 gacgaattga tcaatacgga tatcgttcac acgagtatcc atgaatctac tgttaagggc
     6541 atcagatcta ttttaaaaaa tggccaggaa atgaaagcaa tagatcgaag aaaagagatc
     6601 tacaattttt atgaagtcga agcaagccta cgatatggca tcggaaaatt gtggttagat
     6661 ggaagtaata ttcgtcaaaa caaaccatac attttttgga acagtagtga tttagaactt
     6721 ccaatgaaag ataaataatt atggagaatc gtttgaaaat gattcctcta tacaggattg
     6781 tgcttatagt tggcatggag ttaaaaacct taaagaagga gcagttattc aaagtgaaag
     6841 aacgtcataa tcagttacta tgtagtaaaa cgggtacgat gaacattgaa taccgaacag
     6901 aatatttaga attaacgaat caaactgatt ttttgttgat tcgaaagcaa caagagcaag
     6961 aaaaaaatta atggctactt atagccaatt gattgatgaa tgggaacaat tcaaggaatc
     7021 taaaattcca gaacgtctct aaaagccttt atttgtaaag agcataagga aacgtgggaa
     7081 cactttttaa aaaaagtgtt ccaataggct gtgcctatta cgtttctcaa aaataaatgt
     7141 gggaacaaaa tatttaaaaa taggatagtg aaaggatagt gaaggatagt aaaaggaggg
     7201 tagtaggata gcaaaaagga taggctcctt ttcgctatcc tttttaaatt ggaggaatta
     7261 atatggaagt aagaataaga aatgtggatg agaaaatcgt aagagaaatt gatcgaagat
     7321 gtgacgaacg gttctgttgc aaagttttaa atctactatc aaataaggta gaataataga
     7381 aaaagatggc aggaggaatg acgatgaatc attttaaagg aaagcaattt cagcaggatg
     7441 tgattattgt agccgtgggc tactatcttc gttataacct tagctatcgt gaagttcaag
     7501 aaatcttata tgatcgtggc attaacgttt ctcatacgac gatttatcgt tgggtgcaag
     7561 a
//
LOCUS       DACDPS010000099         6864 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.099, whole genome shotgun sequence.
ACCESSION   DACDPS010000099 DACDPS010000000
VERSION     DACDPS010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.099"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..384
                     /locus_tag="IWJ07_002393"
     CDS             <1..384
                     /locus_tag="IWJ07_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9333929.1"
                     /translation="GQIQSTDFGSTLQEVKAASEKQEAARHLGICPKCQEQEVLLYHK
                     AAACTSEACDFRLWTTIAKKKLTATQLKEIIQNGRTSQPVKGLKGQKGSFEATIVLKE
                     DFTTSFEFSEKKKTNYKKRTRRTTK"
     gene            630..1226
                     /locus_tag="IWJ07_002394"
     CDS             630..1226
                     /locus_tag="IWJ07_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9333930.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYSKAEWYELGKIEIKSRYGNPVIAYDPERTLCDIWNPWYNVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTIRSYIMALN"
     gene            1239..2138
                     /locus_tag="IWJ07_002395"
     CDS             1239..2138
                     /locus_tag="IWJ07_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP9333931.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNDIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFENRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYEETMDSISNLMEKVI
                     HVEEKERKIAYQRHQQMNREMER"
     gene            2141..2626
                     /gene="ssb"
                     /locus_tag="IWJ07_002396"
     CDS             2141..2626
                     /gene="ssb"
                     /locus_tag="IWJ07_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP9333932.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGILIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            2992..3252
                     /locus_tag="IWJ07_002397"
     CDS             2992..3252
                     /locus_tag="IWJ07_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333933.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            3699..3905
                     /locus_tag="IWJ07_002398"
     CDS             3699..3905
                     /locus_tag="IWJ07_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP9333934.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            3905..4129
                     /locus_tag="IWJ07_002399"
     CDS             3905..4129
                     /locus_tag="IWJ07_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333935.1"
                     /translation="MKTQHKLLNVKEVISEKVAEALLIPRYWTIETPLVEDAPTMLFE
                     DLVKIAGYELHYQENLETYENEYWLTKLEN"
     gene            4144..4581
                     /locus_tag="IWJ07_002400"
     CDS             4144..4581
                     /locus_tag="IWJ07_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333936.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEAGVKPSTIKPSEYQALE
                     LTYGKFIETKKAKTVSSEWLDYFENVNKYGACYIMKKEDNENE"
     gene            4574..5281
                     /locus_tag="IWJ07_002401"
     CDS             4574..5281
                     /locus_tag="IWJ07_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333937.1"
                     /translation="MSNVVYQLTVEDVSHFLTNKEHLNEVVRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDCKRMPYQRFERKKGQ"
     gene            5662..5841
                     /locus_tag="IWJ07_002402"
     CDS             5662..5841
                     /locus_tag="IWJ07_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333938.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQELKQKYGKSESRIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            5934..6119
                     /locus_tag="IWJ07_002403"
     CDS             5934..6119
                     /locus_tag="IWJ07_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003027804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, HicA family"
                     /protein_id="HAP9333939.1"
                     /translation="MPLTGKEMLKLLKKNGWVERRQEGSHHHLYKDGVRITVPVHANQ
                     DLGRGLEQKILKDAGLK"
     gene            6153..6584
                     /locus_tag="IWJ07_002404"
     CDS             6153..6584
                     /locus_tag="IWJ07_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HicB family protein"
                     /protein_id="HAP9333940.1"
                     /translation="MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYG
                     IAMAQEVLGMVLADYIEHEDLLPEPTPINKISVEDDSFTTLIRVDVAKYLKDTELVKK
                     TLTIPQWADKLGKRAGINFSVLLTESIADKADNILRSGRNN"
BASE COUNT         2460 a         1152 c         1410 g         1842 t
ORIGIN      
        1 ggacaaatcc aatccactga ttttggatcg acgttacaag aggtgaaagc agccagtgaa
       61 aagcaagaag ccgcgcgtca tttagggatt tgtccaaaat gccaggaaca agaagtttta
      121 ctctatcata aggcggccgc atgtacgtct gaagcttgtg attttagatt atggacaacg
      181 attgccaaaa agaaactcac agcgacacag ctgaaggaaa tcattcaaaa tggccgaaca
      241 agccagccag tgaaaggatt aaaaggtcaa aagggttcgt ttgaagcaac gattgttcta
      301 aaagaagact tcacaacgag ctttgaattt tctgaaaaga aaaaaacgaa ctataagaaa
      361 agaacacgcc gcactacaaa ataatggagg ggttatctta tgcgaaatac aacacacaaa
      421 caacaagaaa tcgaaaatca aaaagcaaaa attcgttcag aacaagagaa attacgtgcc
      481 agtaaaagaa gacttcagca attaaagaac cagcaacgga ttcaaaaaga aattgaatta
      541 gatcgataaa atcttcctgg ctattgtata cgcataaact tatgttatac tatatttata
      601 agtttatacg tatacaaagg agggagaaaa tgacaaaagc aatggtttac aagctgctag
      661 aagaatttaa cggaactttg gctttgaagg atgccaaaaa agcgggcatt tctcctgtaa
      721 cgatcaaacg tatggtagat cgtggagagc tggatcgtga gtatccaggt tttttcacct
      781 tacctggtca atttcctgat gaattgttta tggcacaaaa gaaatatgag cgcggcatta
      841 tttcgcatat caccgcgttg gatttatatg atttaacgga tatgatccct agacagatcg
      901 atctaaccgt accttacggt tatcacgttt ctgaaaaggg gttgaaagaa ttcgcagttg
      961 agttacacta ttcaaaagcg gaatggtatg aattaggtaa gatcgaaata aagagtcgtt
     1021 atgggaatcc tgttatagct tatgatccag aaagaacctt gtgtgacatt tggaatccct
     1081 ggtacaacgt tgaggatgaa atcaaagtaa aagcaatcaa aaactacatg gagtcggaca
     1141 gaaaaaatct tagaaaattg aatgagtatc gacgaatttt gcctacagac aaaacgatac
     1201 gctcttatat tatggcttta aattaaagga gaagacacat gacacctacg caattaaaag
     1261 caaaagtgaa gaatatttcc agagaaaaag aagttgatcc gcagcttgtt ttaagacatt
     1321 ttatgatgga aaaattcttg gaaaaaatct cagagtctcc ttatcgtgaa aattttgtgt
     1381 taaaaggcgg cttcttaatt ggctcaaagt atggcattga aaatcgaaca acaaaagaca
     1441 ttgatacgac tttacgggaa atgaaagtaa ccaaagaaac attgacaacg gttctaaacg
     1501 atattttttc aacgccgacg aaagaaggca ttcaatttga gattcaagga atgaaagaaa
     1561 caagggaagc tgattattat ccagggttta gcttacgtgt actggcacat ttggaaaaca
     1621 tgcggccaga ttttaaggta gacgtgacga caggtgactc gatctatcct gcaacgatca
     1681 ctcacagtca taaattgatg tttgaaaatc gaacgattcc tttggaatct tatcctacag
     1741 aacaaattat tgccgaaaaa ttaagtgcaa cctttgattt tttaacagat aattcgagag
     1801 gaaaagattt ttatgatctc tatactattc ctaaaatgga aaagatcgat gagaagacct
     1861 tgaaagatag tgtgagaaat acatttacac gtcgaggaaa aactgatccg ttaaagattt
     1921 attacgaaaa ggatatggat gtactgaaaa atagtcatga gctaaaagat acctggaaga
     1981 aatatcaaaa aagcaatcct tatgctgcgt cgatcacgta cgaagagaca atggattcga
     2041 ttagtaattt gatggaaaaa gtgattcacg tcgaagaaaa agagcgaaaa atagcttatc
     2101 aaagacacca acagatgaac agagaaatgg agagataaaa atgatcaata atgtaacttt
     2161 agtaggacgg ttaaccaaag atccagattt acgttatacc gcaagtggca cagcagtggc
     2221 tacttttacc ctagccgtta atcgtaattt cacgaatcaa aacggcaatc gagaagctga
     2281 ttttatcaat tgcgtgattt ggcgcaaacc agcagaaacg atggcgacat tagctaaaaa
     2341 aggcatcttg atcggcgttg tcggacggat tcaaacacgg acttatgaca accagcaagg
     2401 acaacgtgtg tatgtgacag aagttgtagc tgataacttc caactattag aaagcaaagc
     2461 agcgacagag agccgtgcgc acgctgatca atcgtccacg agtccaagca ccaccacttt
     2521 tgaacaacgg gatacagcga cgcctaacaa caatggattg aacgcttcac agaatccatt
     2581 tggtggacaa tcaatcgata ttagcgatga tgatctaccg ttttaagtag cattttctat
     2641 tggttgcatt tgctacggtt tatgttataa tttagtcaac aaaaagaatt ttcaatctat
     2701 aaccaaaaac cactagctgg cgggctagtg gttttctttt acgtttctat tataagaatt
     2761 aacgagtagg cttactgtct ttcttggcaa atttacaatc aagccatttg cagatatagt
     2821 gcgcggctac ttctgccatg attgcaaaaa caaaatcaaa aagaattttc aaacctataa
     2881 cctcctcccc ttgccagatt aggccggggt agcagacagc aagactatta tagcataatt
     2941 gaaatatcca aatgattgga tttttgctat aattttagcg aggtgattgt gatgaacaaa
     3001 tatgttggtt tgcttgataa aatccgagta ataaaaacgc aaccgttatt ggttcgcttt
     3061 actttacaaa cgattcatga atcgattaat tgtgtagttg cagatattga aattatcgat
     3121 aaattgctaa ttatggatga tggaaaatac aatattgccg tcacaggtca cttcaataaa
     3181 cgaaatcagc tggttattgc tagcatgtat gttagaaatc ccgatcattt tacacgatcg
     3241 atgggcatat aaataaaaga agagttgtag tcctaaatga ggattgcagc tctttttgtt
     3301 ttacatagaa acgggaacag gaaaaggcct gtttaggaag gcacaagcgc cttctcaatt
     3361 aaaaaacctt tctctcactc caaccgccac cgccgttcga gaagaaagga agtgaacgct
     3421 gcacgttcac taagatatgg tgtcttgcaa agcaagacaa aataggaaaa gcttcctctc
     3481 accaaaaggt tcgttcctgt tcgctatctc tttattgatt tgccagaccg gcaacaagtt
     3541 gccttttatc accaaaaagc gacaaggaca agggtaaatg gcacgctgcg cgcccttgtc
     3601 cttgtcgctt ttcgttgatt cattctggcc aacaaatcaa taaagaaaga actaacgaaa
     3661 ggaagtaaaa cctttcctaa gaggaggcag aatcaatcat gcgaaagaaa tttgattatt
     3721 ggggtgttcc cttcagcgaa ttattcccaa actatcatgc accacatact gtcgaatgcg
     3781 attgtggaga aagagcgaaa tgtatcaaaa gctatcgttt gtatcaatgt ccaacatgcg
     3841 ggaaaaaata taccttgtct tatggcgatt acattttgat tgatgagaaa ggaaagaaac
     3901 gctgatgaaa acacaacaca aattattgaa tgtaaaagaa gtcatttctg aaaaagtagc
     3961 agaagcttta cttattccgc ggtactggac gatagaaacc cctttagtag aggacgcacc
     4021 gactatgcta tttgaagacc tagtgaagat tgcaggatat gaactgcatt atcaagaaaa
     4081 cttggaaact tatgaaaacg aatattggtt aaccaaatta gagaactaaa aaggagaaaa
     4141 aatatggctc agaattacta tgacgaattt gtcaaattac ctttggataa aatggcacaa
     4201 aaaatggagg atatgacgtt cctataccat gaaacacgtg tacccaaaaa acattacaag
     4261 gaaaaattgt cagtcgcagt ggaagaaatg attgaatcag gtgtcgaaat gaatctcatt
     4321 gcgacgtact atcgaacatt ggaagaactg aaaaaacaaa atggaaaatg gtttttccaa
     4381 gccttactat gcttggaagc aggcgtcaaa ccaagcacaa tcaaaccttc tgaataccaa
     4441 gctttagaac taacgtatgg aaaatttatc gaaacgaaaa aagccaagac tgtttcaagt
     4501 gaatggttgg actactttga aaatgtcaac aagtatggcg catgttacat catgaaaaag
     4561 gaggacaacg aaaatgagta atgtggttta tcaactaaca gtggaagatg tttctcactt
     4621 tttaacaaac aaagaacatt tgaatgaagt agtcagacgg attcaaaaag actttagttg
     4681 tatggaagaa atgaagtact ttattcaaag agtggaagaa cagttgtata tggaaagcga
     4741 aacgagtcaa acgaaggtca cagtcagcca taatcattta gacgtgtata aacgtcgtcc
     4801 tgaaacggta aaaaaaggaa cagagcttct acgactagaa acagaaaaat acatcatgaa
     4861 tgtgtaccta gaaaggagtg agctggtcga aaactttgtc agctacacga tttgtaggaa
     4921 acactattac tcagaatcag aatattattc aatttttgag agcatggaat taatggaaga
     4981 agaaatggaa gagctaaaca gcaaaaatgc gacggtagac gattattacc ttgtatttac
     5041 tagatttctg acgtgtggga cggaatctat cgtgttaatg ggaatggatt atgaactcgc
     5101 tgtacccgaa gcatttttga gggaatatgt agaagaagta gataacccag gcgacgactg
     5161 gtacacgact ttaactgatt ttttagatac ctacacttac gacgatacag aagaaatcca
     5221 aaatgagctt gactgtaaac gaatgccata tcaacgattt gaaaggaaaa aaggtcaata
     5281 accatttata ccgaaaagtg agaatgtgtg aaataagaac aaaaaacctt gaaattaagg
     5341 gaatggttct tatttcgcac caatcgctcc aaccgccccc gccgctcagg tggtgcgaaa
     5401 taagcgggca acgaccgata acctaagaga aagaaggaaa tccatatgaa taaaatcatt
     5461 cctctgttgt tcgtactgtt tgttggcgag atacttatgc gtcatgctgc agctgtacat
     5521 ttaaacttcg tagtattctt aggaatactg gtattttgtt tgatcttttg gcgatttgta
     5581 ttagcccttg tgagtctact attcaagatg attgcagcca tatttttcat agggttattt
     5641 cgatgaaagg agagagtact aatgccaatt gcaataggaa ataaacgttt acccgtgacg
     5701 ttggatgaaa agagacaaaa agaattgcag gaactcaaac agaagtacgg caaaagtgaa
     5761 tccaggatta tgtgtattgc gttagattta ttgattgcac aagaaaaagc agggtttgat
     5821 gtaccagcac tcaaaaagtg acgtcacttt tcatttgaag aaacaaaagt gatagcactt
     5881 tacgcacgtg tcgatacgtg ttatgattat tttgtaagga ggtatagtgc acaatgccac
     5941 ttacgggaaa agaaatgctt aagttattga aaaaaaatgg ctgggttgag cgaaggcaag
     6001 agggatctca tcatcatctt tataaggatg gtgttagaat cactgttcct gttcatgcca
     6061 atcaagatct tggccgagga cttgagcaaa aaattttaaa ggatgcggga ttgaaataat
     6121 ctcgcattcg attaaagaaa ggattgactg aaatgttaag tgagccttta gcaaaaacct
     6181 atcctgctat ttttagccca gaagaaggtg gtgggtactt tattgaattt cctgatgttc
     6241 aaggtgctta cacaggaatt aacgaagacg atatatctta tggaattgct atggcacaag
     6301 aggtgttagg tatggtttta gcagattata ttgagcatga agatctactt ccagaaccaa
     6361 caccaatcaa taagatttct gttgaagatg attcttttac tactttaatt cgtgtagatg
     6421 ttgccaaata tttgaaagac acagaacttg tcaaaaaaac cttaacaatt cctcaatggg
     6481 cggataagct aggcaaaagg gctggcataa atttttcagt ccttctaaca gaatcaattg
     6541 cagataaagc agataacatt cttcgttctg gaagaaataa ttagaactca aaagagtcag
     6601 aaacttaaaa atctttggct tcttttttta ctacaaagat ggcttcttta aattatttgc
     6661 ctgattgatt taaaaaaatt gcgcaaagac tacgagctaa ttaagttgct cgtggtcttt
     6721 ctttatgaaa actttttaga tctagataaa aacattgaga cactttgaaa aattttttta
     6781 atagtaatgg gcgcttctat gtcaagctga ttcttctttc ggtaaattaa ttttacttag
     6841 aatatcacta tcttgataaa aaat
//
LOCUS       DACDPS010000100         6791 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.100, whole genome shotgun sequence.
ACCESSION   DACDPS010000100 DACDPS010000000
VERSION     DACDPS010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.100"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(239..496)
                     /locus_tag="IWJ07_002405"
     CDS             complement(239..496)
                     /locus_tag="IWJ07_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP9333941.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(489..758)
                     /locus_tag="IWJ07_002406"
     CDS             complement(489..758)
                     /locus_tag="IWJ07_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP9333942.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(889..1068)
                     /locus_tag="IWJ07_002407"
     CDS             complement(889..1068)
                     /locus_tag="IWJ07_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333943.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(1449..2156)
                     /locus_tag="IWJ07_002408"
     CDS             complement(1449..2156)
                     /locus_tag="IWJ07_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333944.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            complement(2149..2586)
                     /locus_tag="IWJ07_002409"
     CDS             complement(2149..2586)
                     /locus_tag="IWJ07_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333945.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            complement(2601..2852)
                     /locus_tag="IWJ07_002410"
     CDS             complement(2601..2852)
                     /locus_tag="IWJ07_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333946.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            complement(2852..3058)
                     /locus_tag="IWJ07_002411"
     CDS             complement(2852..3058)
                     /locus_tag="IWJ07_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP9333947.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            complement(3495..3755)
                     /locus_tag="IWJ07_002412"
     CDS             complement(3495..3755)
                     /locus_tag="IWJ07_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333948.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            3830..4033
                     /locus_tag="IWJ07_002413"
     CDS             3830..4033
                     /locus_tag="IWJ07_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAP9333949.1"
                     /translation="MIKTVKFVTVAIRLKVRWCVCAYFQNLTERRSLLPVETALGLMI
                     SFGSLIATIIFGILAVVNNNQKK"
     gene            complement(4174..4659)
                     /gene="ssb"
                     /locus_tag="IWJ07_002414"
     CDS             complement(4174..4659)
                     /gene="ssb"
                     /locus_tag="IWJ07_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP9333950.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(4662..5561)
                     /locus_tag="IWJ07_002415"
     CDS             complement(4662..5561)
                     /locus_tag="IWJ07_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP9333951.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(5574..6167)
                     /locus_tag="IWJ07_002416"
     CDS             complement(5574..6167)
                     /locus_tag="IWJ07_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HAP9333952.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(6408..>6791)
                     /locus_tag="IWJ07_002417"
     CDS             complement(6408..>6791)
                     /locus_tag="IWJ07_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HAP9333953.1"
                     /translation="GQIQSTDFGSTLQEVKAASEKQEATRHLGVCPKCREQEVLLYQN
                     VAACTSEACDFKLWTTIAKKKLTATQLKEIIQNGRTSQSVRGLKGQKGSFEATIVLKE
                     DFTTGFEFSEKKKTNYKKRTRRTTK"
BASE COUNT         1828 a         1349 c         1086 g         2528 t
ORIGIN      
        1 tacaatagta ttataacata tctattcaac ttcaaattgt tgaaatttaa gccatttata
       61 tttacttttc ctcaacttta gaaatgattt tactaacctt atatccgtaa tacgcgtaac
      121 aaacacattc attaataaca atcaacacta agctatatta gctttttaaa tgactagggt
      181 aaataaaata aggatcaaaa acttttaagt ttctgaccct ttccttactt ccgattggtt
      241 aatagtgatc ttttgcagaa taaataaata tcgtttcatt ttcaactcta tatatcagtc
      301 tatgttcatc tgtaattctt ctggaccatt ttccagataa atcatgcttt aatggctcag
      361 gttttcctaa tccagcaaag ggggaacgat cgatatcttt tattaacttg ttaatctttt
      421 ttatattgct tttgtttcct tgctcatgcc aataaagata atcatcccaa gcatcatcag
      481 accaagcctt aatcatcaga ttcaacctcg attaagtcat gtgttttaaa tgcacctttg
      541 gagaattgtt catctcctcg acgaattttt tccatgacgt aattattaga aagtgttctc
      601 aacgtttctt gcatagaatc ataatctctt tttgataata caacaactgt atcttctaca
      661 tctttacttg ttacaataag tgtttcagca tcctcattaa cttgtttcat ataactacgt
      721 aaattttggc ggaaatttga ataagctact gcttccattc ctttcacccc ttatttattt
      781 ctataaaaca attgtacatt atattgtaca attaagcaaa tgattaatat tctaacttct
      841 tataattaaa agtgctatca cttttagttt ttaggataaa aagtgacgtc actttttgag
      901 tgctggtacc tcaaatcctg ctttttcttg ggcaatcaat aaatctaacg caatacacat
      961 aatcctggat tcacttttgc cgtacttctg ctttagttgc tgcaattctt tttgtctctt
     1021 ttcatccaac gtcacgggta accgtttatt tcctattgca attggcatta gtactctctc
     1081 ctttcatcga aataacccta ttaaaaatat ggctgcaatc aacttgaata ttagactcac
     1141 aagggttaac acaaagcgcc aaaagatcaa acaaaatacc agtattccta agaatactac
     1201 gaagtttaaa tgtagagctg caccatgacg cataagtatc tcgccaacaa acagtacgaa
     1261 caatagagga atgattttat tcatatggat ctccttcttt ctcttaggtt atcgggcttt
     1321 gcccgcttat ttcgcaccac ctaagcggcg ggggcggttg gatcgattgg tgcgaaataa
     1381 gaaccattcc cttaatttca aggttttttg ttcttatttc acacatcctc acttttcggt
     1441 ataaatggtt attgaccttt tttcctttca aatcgttgat atggcattcg tttacggtca
     1501 agttcatttt ggatttcttc tgtatcgtcg taagtgtagg tgtctaaaaa atcagttaaa
     1561 gtcgtgtacc agtcgtcgcc tgggttatct acttcttcta catattccct caaaaatgct
     1621 tcgggtacag tgagttcata atccattccc attaacacga tagattccgt cccacacgtc
     1681 aaaaatctag taaatacaag gtaataatcg tctaccgtcg catctttgct gtttagctct
     1741 tccatttctt cttccattaa ttccatgctc tcaaaaattg aataatattc tgattctgag
     1801 taatagtgtt tcctgcaaat cgtgtagctg acaaagtttt cgaccagctc actcctttct
     1861 aggtaaacat tcatgatata tttttctgtt tctagtcgta gaagctccgc tccttttttt
     1921 aatgtgtcag gacgacgttt atacacgtct aaacgattat ggctgactgt aaccttcgtt
     1981 tgactcgttt cactttccat atacaactgt tcttccactc tttgaataaa gtacttcatt
     2041 tcttccatac aactaaagtc tttttgaatc cgtctgatta cttcattcaa gtgttctttg
     2101 tttgttaaaa attgcgaaac atcttctgtt gttagctgat aaaccacctt attcattatc
     2161 tttctcctcc gattcaatcg tataaaatgc accatttttt tcgatatttt caaaagtatc
     2221 tagccagtcg ctagccattg ctttagcttt cttaatctgt gcgaattttg cataagtcag
     2281 ttctaaggct tgatattcgg aaggtttgat agtgcttggt ttgacaccta cttccaagca
     2341 tagtaaggct tggaaaaacc attttccatt ttgttttttc agttcttcca gtgttcgata
     2401 gtacgtcgca atgagattca tttcgacacc tgattcaatc atttcttcca ctgcgactga
     2461 caatttttcc ttgtaatgtt ttttgggtac acgtgtttca tggtatagga acgtcatatc
     2521 ctccattttt tgtgccattt tatccaaagg taatttgaca aattcgtcat agtaattttg
     2581 agccatattt tttctccttt ttagttctct aatttggtta accaatattc gatttcataa
     2641 gtttccatgt tttcttgata atgcagttca tatcctgcaa tcttcactag gtcttcaaat
     2701 agcatagtcg gtgcatcctc tactaaaggg gtttctatcg tccagtaccg cggaataagt
     2761 aaagcttctg ctactttttc agaaatgact tctttgtcca ccaaaatttg aacgacttct
     2821 tttacagtca ataatttgtg ttgtgttttc atcaccgttt ctttactttc tcatcaatca
     2881 aaacataatc gccataagac aaggtatatt ttttcccgca tgttggacat tgatacaaac
     2941 gatagctttt gatacatttc gctctttctc cacaatcgca ttcgacagta tgtggtgcat
     3001 gatagtttgg gaataattcg ctgaagggaa caccccaata atcaaatttc tttcgcatga
     3061 ttgattctgc ctcctcttag gaaaggtttt atttcctttc gttagttctt tctttattga
     3121 tttgttggcc agaatgaatc aacgaaaagc gacaaggaca agggcgcgca gcgtgccatt
     3181 tacccttgtc cttgtcgctt tttggtgata aaaggcaact tgttgccggt ctggcaaatc
     3241 aataaagaga tagcgaacag gaacgaacct tttggtgaga ggaagttttt tctattttgt
     3301 cttgctttgc aagacaccat atcttagtga acgtgcaacg ttcacttcct ttcttctcga
     3361 acggcggtgg cggttggagt gagagaaagg tttttcaatt gagaaggcac ttgtgtcttc
     3421 ctaaaaaggt cttttcctgt ttctatgtaa aacaaaaaga gctgcaatcc tcatttagga
     3481 ctgcagctct tcttttatat gcccatcgat cgcgtaaaat gatcggggtt tctaatttgc
     3541 atcgattcaa tgactaattg attccgttta ttgaaatgac ctgtgacggc aatattgtat
     3601 tttccatcat ccataattaa caatttatcg ataatttcaa tatctgcaac tacacaatta
     3661 atcgattcat gaattgtttg taaagtaaag cgaaccaata atggttgtgt ttttattact
     3721 cggattttat caagcaaacc aacatatttg ttcatcacga tcacctcgct aaaattatag
     3781 caaaaacagc taaaattgag aatttttatt cgattttaga tatgctataa tgatcaagac
     3841 agtaaagttt gtgacggtgg ctatccgatt gaaagtgagg tggtgcgtat gtgcgtactt
     3901 ccaaaatctt acagaaagga gaagcctttt gcctgttgaa acggcgctag gattaatgat
     3961 tagtttcggt tctttgatcg caacaatcat ttttggaatc ttagcagttg taaacaacaa
     4021 tcaaaaaaaa taaccgtcag ctttagccgg tcgacgatta tttttcaaaa ataatattaa
     4081 gctggtcacc gtcttaaacg gtgctgttag gggagttgtt agcgcaactc ctcttttcgt
     4141 atccattata acaactttat tgatctttta caatcagaat ggtaaatcat catcactaat
     4201 atcgattgat tgtccgccaa atggattttg tgaagcgttc aatccattgt tgttaggcgt
     4261 agctacatcc cgttgttcaa aggtggtagg gcttggactg ctggacgatt gatcagcgtg
     4321 cgcacggctt tctgtcgctg ctttactttc taatagttgg aaattatcgg ccacaacttc
     4381 tgtcacatac acacgttgtc cttgttggtt atcgtaggtt cgggtttgaa tccgtccaac
     4441 aacgccgatc aaactgccct ttttagctaa tgtcgccatc gtttctgctg gtttgcgcca
     4501 aatcacacag ttaataaaat cagcttctcg attgccgttt tgattcgtga aatttcggtt
     4561 gacagctagg gtaaaagtcg ctactgcagt accactcgcg gtataacgta aatctggatc
     4621 tttggttaag cgtcctacta aagttacatt attgatcatc tttatctctc catttcttga
     4681 ttcatttgtt gtgatctttt aaactgccgt tttctttctt gattttcaat tttgatcaca
     4741 cttttcatta ggtgatcaat ttcgttgact gtatctgtaa atgaaatgga attcgcaaac
     4801 gtgttaactg agcgatattt ttcccactgc ttttccaaat agtcatttcc tctgatctta
     4861 tccatttctc tttcataata aaaggacaac ggttttgtat ccccacgttt tgtaaatgta
     4921 tttcgaacac ttttgtataa ctctttcgta tcgatatttt ctagtttcgg aatggtaaat
     4981 aagtcgtagt aatcttttgc tctcgaatta tctgtgccaa agctgaacgt agcacttagc
     5041 ttttctgcaa taatttgttc tgttggataa gctggaatct ccactttttt atcttcaaac
     5101 attaattggt gatagtgtgt atctacttca ggataaatag aatctcctgt tgtcacgtcc
     5161 attttaaacg gtacacgtac tttctctaaa ttagccacca atttaatttg aaatccagga
     5221 taaaaatcgg cttctcttgt ttccttcatt ccttgaatag agaaaacaat gccttctttt
     5281 gtgggttttc tagttaaatc attaaaaatg ctcttcaatt tttcttcagt taatttgcta
     5341 tttctaaatg tcgtatcgat atcaattgtt gtccttctat cgattccata ttttgatcct
     5401 aataaaaagc cgcctttcaa aatgaagtca tttttgtact ttgattcaga aatttgttca
     5461 attagacgat ccatcataaa atgccgttgc actaactgtg catttaatcc tgtactcttt
     5521 gctaaatttc ttgtttttgc tgcaaattgt gtcggtgtca tctttttcct ccatcaatat
     5581 aagctttgca tgtggtattt catttctttt tctacatgta gttttgtcat gtaatctctt
     5641 aatttacttg gatctctcaa tgggtcttcc atgtactctt ttaatgcttc taatttcatt
     5701 tcatattcaa attcatatct cggattccaa atatcacaaa gtgtacgctc tttatcatac
     5761 atagtaatag gatttccttg aaaggaagtt ccctctactt ttcctagttc ataaacttct
     5821 ttcttcacga aatgaaattg taactgctgc tcttttgtaa ttgaacgatc aacgttatag
     5881 ccatggggaa aagttaagtg aatgaatttc ggcatattcg tagaaagctg gtaaatatct
     5941 aaagtcgttt catgagagaa gattcccctg gaaaatcgtt gctgaagcca aaataaatca
     6001 tctattccat gaccatcctc tacataataa ccacgatcaa ctttgatcaa ttttccttga
     6061 ttcaccaatc tttgtacagt ggttgctgcg attccaaatt tcctggcttc cttcatagat
     6121 aaatttccat cgcgaatagc caacaattct ttcagtttat cttgcattta cttcccctcc
     6181 cttcgttaca aatacactac atcaaatata tcatagtagt ttatttgtaa caagattaac
     6241 gtctatcgat ctaattcgat ttctttttga atgcgctgct ggttctttaa ttgttgcagt
     6301 cttcttttat ttgcacgtaa tttctcttgt tctaaacgaa tttttgcttt ttgattttcg
     6361 atttcttgtt gtttgtgtgt tgtatttcgc ataggataac cccttcatta ttttgtagtg
     6421 cggcgtgttc ttttcttata gttcgttttt ttcttttcag aaaattcaaa gcctgttgta
     6481 aagtcttctt ttaaaacgat cgttgcttca aacgaacctt tctgaccttt tagtcctctg
     6541 actgattggc ttgttcggcc gttttgaatg atctctttca gttgtgtggc tgtgagtttc
     6601 tttttggcaa tcgttgtcca taatttaaaa tcacaggctt cagacgtaca tgccgccaca
     6661 ttttgataaa gcaagacttc ttgttcccga cattttggac aaacacctaa atgacgcgtt
     6721 gcttcctgtt tttcactggc tgctttcacc tcttgtaacg ttgatccaaa atctgttgac
     6781 tggatttgtc c
//
LOCUS       DACDPS010000101         6785 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.101, whole genome shotgun sequence.
ACCESSION   DACDPS010000101 DACDPS010000000
VERSION     DACDPS010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.101"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(718..3867)
                     /locus_tag="IWJ07_002418"
     CDS             complement(718..3867)
                     /locus_tag="IWJ07_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP9333954.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3880..5079)
                     /locus_tag="IWJ07_002419"
     CDS             complement(3880..5079)
                     /locus_tag="IWJ07_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP9333955.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLIDLVVRLNKSTNSSRLPKVEF
                     EDIVAGEGRLNKDVSQKFDNRKGIEFLPNDILYGKLRPYLKNWLKATFTGVAIGDFWV
                     FRAKKSDSDFIYSLIQADRYQKAANDTSGTKMPRSDWKKVSDTVFYIPNDLKEQQKIG
                     IFFKKLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWEERKLGE
                     LGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIERKKWKPI
                     KDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFETINLPR
                     YSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLLKETKKS
                     FLQKMFV"
     gene            complement(5069..6664)
                     /locus_tag="IWJ07_002420"
     CDS             complement(5069..6664)
                     /locus_tag="IWJ07_002420"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP9333956.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEMWLQGALEVFKHEQ"
BASE COUNT         1905 a         1398 c         1101 g         2381 t
ORIGIN      
        1 ggactaatac aggtagtcct cttttttgtt attttgatat tctaaatcat tttttattgg
       61 attattccct tgttcgatat agacccaaat tgatctatca ataattatca aactgagtca
      121 tatagagaca aaaatttcaa aaatgacctg ttaaatggtc atcgaatatc atttaaaatc
      181 aatacgacgg agtcagacac ggagtcaaaa caagcccgct gagagtaaaa aatatataca
      241 gtcccatagg taaagtgcca caggactgta tattttttta tttgagatta cctgaaatga
      301 ttccatttac tttttatctc attttttcct actagttagt atcaaatact ctcctatttt
      361 ctcaataaac tattttgggt tacatggtat ctgaaacagc taaaaccttg tcattacagc
      421 aaaaaatcac tttatctccg tttcatttca gatacatttc acttaagcgg attccaaaaa
      481 aacaaaagaa aaaggaaccc ctagttagcc tatgcttttt attggttcct ctcaaaacac
      541 ggtcatataa caacggggtg gggtaaatga atgaaaaagg tggaggaaac attattcaat
      601 agctgtctta aatacttcgt tataaaatgt gaacaaataa aagacatcta tccctatttt
      661 ctacacttcc actttactac catctttgaa tcgtacaatt ttcttaactc ttctcgttta
      721 cctcaattca ttttcaagtg gaatcagctc atcatctaac attttacgcc atgctcgctt
      781 catttcttgt gggtatttaa atggcttcgc ttctggatgg ttggctttat atccttcgta
      841 atccttactg ttgataattg ctttcatatt aggaattgga tcttcacctg gcatatattg
      901 cagattagaa gcatgtaatt ctttctcaga aacaaaccac tcattggaaa agacatgaat
      961 caaatcatca ctcgcttgtg taaaatagcg acgtttaatg acaaagatat cttcattact
     1021 atctaaagtc cccacatcaa acgcatttaa aatttgtcgg taaacttctt tgactccttc
     1081 tgttttattc aatttagcca aagctttttc aatttcttct cgtgcatctg aactattcgc
     1141 cgcataagtt cctagtaatt gatcaatata agtagaatca atatcataca acttcgtggt
     1201 attttcacta taaaattcga tcgtggaaaa atcttctccc tctttttctg gttcttcctc
     1261 tattaatgaa cccttcacgg tctcgtacac gccaatttgt tcttccaaaa gttgaatttg
     1321 attgttcaag gttgaagatt tcaccatgtc atcattgtaa tcgtcataag tcactaacga
     1381 ttcataagca cgattcaatt cttggaatgc tttgacgtac tcgatacgga tttcaagtgg
     1441 cgtatgttca tcaatggatt ccatggatga aacctctttt aacttaccat atgcttgctt
     1501 gaatcttttt ttggattcct gataacttgg ataaatcaaa cccgattctt cttttgcttc
     1561 tgaatacagt tttgtcgcat cctcgacatt cttctccatt gtggctggct ttcggaaagt
     1621 aacaattagt ccttttgttt tttctggata cgtacggttc gtacgtgaaa aagcttggat
     1681 caatcctgca tactctagat tacgatccac aaatagggtt tgaatcgtag gtgcgtcaaa
     1741 acctgtcaat agacgatcca ctacgatcac taaatcaatt tgtttcccaa attgtttgaa
     1801 ttccgctctt tttcgtgcca aacgattatt gatgtctccg ttataacgct caatgtctgc
     1861 taatgaccag gaagtatcat aatactgatt atattcctca ataatttttt gcatttcttc
     1921 ttgttgggca cttgaatctc ggctattttc ttctaatgaa taggtaatag cgattcgtgg
     1981 aaaatcagga tcctccatcg ttcgtccttc acgaatgagc tgattgggaa actcttttgt
     2041 taaccaatca ggctctttcg tcatctcttt gatggcccga taatactttt tggccatgtc
     2101 aatggaactc gtggttaaga ttgcagattt cgttggcctg ccatttcgaa aatcaaattt
     2161 tgtataggca ttatctggac ggaacatttt atgaatgact ttttggatat gttcatctgc
     2221 ttcaaaaaca gctgggtcca aataactttc ttttttgaca ggttccattt gatcaatcat
     2281 acgattgatc tgttctgatg aatactctgc atatgtttct acttctttta atttccgata
     2341 gattttattc tctagtgaag tcggctccac ggtgttttca tgctctactt gaaatcctaa
     2401 gacagagcca tcttctagcg cattcttaat ggtataggta tgcaagacct ctccatactg
     2461 atcatgggtt gtacgtgcca attgcccttt ggcttgtttc cgattctcag gaaaaatcgg
     2521 tgtacctgta aagccaaacc aagtggattt cggaaacatt tttttgattt cttccatatt
     2581 ttcagcactt aacgctcgat gacactcatc tactacaaac acgatatgtt ggcccattaa
     2641 tttttggaag cgattggttc cttttttctc ttcttgtttc tttgcatatt tcaacgccgc
     2701 atctagtttt tgacgggttg taatgattac aacatttgca ttggcatctg ccaacagggt
     2761 ttcacttaat tctttggcac ttcccgttcc aacgatcaag ctattcgctt tggcgttccc
     2821 ggaagaaatc ccagtattaa attccgaagc aaattttgta aactctgtgg tggtttgatt
     2881 atctaaatct ttgcggtcca ctaacatgat cgttcgatcg atccccgatt ttcgtgctaa
     2941 aagcttagta gatacaaaac tagtcaacgt ctttcctgaa ccagtcgcat gccacacata
     3001 ccctgattga tgtttatttg cggcaataaa caaggcttga atcgcatgta cttgataagg
     3061 atgtaacacc atgagggttt tattatcttg atcttcactg acgattgtat aatccgcaat
     3121 caaacgatga gcatccggga tatttaacac ttgtttacaa aactcatata agttttctac
     3181 tttttcattg tctttcgttc gccaactaaa taaaaactta gggtgcaagt ctttcggtaa
     3241 ggcatttgca aaataccgag tggtttgttc attggaaacc acaaacaact gtaaggtcga
     3301 aaagatattg ttccgaaaca taccttcttc tgcatatttt ttgatttggt taaatgcctg
     3361 ataaacacca tcttttgcag ttacttgttt cagctcgatt tggacaatcg gtaatccatt
     3421 gattaataag gtgacgtcaa aacgtcgatc ccgtgcttct atgttacttc cacgttttgc
     3481 aatttgatgg accacttcat aagtagagat tcctccacca atgtcttgat tggaataaag
     3541 gataagtgag acagaaccca attgcgaatc ttcccgttca atcgtgatcc gagccatccc
     3601 attttcacct tttaaccatt tcgcagcatc aaatggggtt ttcgttcgca gcagtaattc
     3661 cgttttgatc gtttcaaatt ctttatcggt caaaggaaaa tcattcagtt ctgcttgatt
     3721 atttgagata attttttggc gtaaattgac ccaaaggtct tcttctgatt ttagatccgg
     3781 acgatacgtc cactgattat gcccttctcc aagtacacga atcaactgtt cttcgacttc
     3841 tgcttcctca cggtgactaa ttttcattct tcttccctcc tagacaaaca tcttttgtaa
     3901 gaagcttttt ttcgtttctt ttagtaaatc taacttacgt tgatgaagag cgatcgtatc
     3961 atctagctgt ttgaagaaag taccgatttt ttgttgttca tcctttacag gaaccttaac
     4021 actgatattc tcaatatcag aactgttata agaaggcaat gcccctactt gggagtatct
     4081 tggcaaattt atagtttcaa acaatatttt accaaaatag atatcccaag tattattaaa
     4141 taaataagcc atggtattgt tatctattaa gaaagggctt gtgaccaaac gttttctatt
     4201 aagcattatt gctgcgccaa ctttagcaaa tataatcgct ggcacatctt ttattggctt
     4261 ccatttttta cgctcaattt gctcattaga tacataatga tttgcatttc tcatttcata
     4321 ctcattacca atgttattca tgtcggaaac tttaaaaaat ggagtacctt cagaaccacc
     4381 ttgctcggca tcaggaaatc caattcccga ttgagtttta cctaattctc ccaacttacg
     4441 ctcttcccaa tcttccgtaa aaccaggaaa ccggatttct ggcacttttg ctccgttttc
     4501 agggaacatt ttttgtaaga atcctttttt cgtttccttc agcaaatcta acttacgttg
     4561 atgaagagcg atagtgtcgt cgagtttttt gaagaatatg ccgatttttt gttgttcttt
     4621 taaatcattg ggaatataga atactgtatc tgaaactttt ttccagtctg aacgaggcat
     4681 ttttgttcca gatgtatcat ttgcagcctt ttgatatcta tcagcttgaa taagagaata
     4741 aataaagtcc gagtcgctct tcttagctct aaaaacccag aaatccccaa tcgctacgcc
     4801 tgtaaaagtt gccttgagcc aattttttaa atatggacgt aactttccat atagaatatc
     4861 atttggtaaa aattcaatcc cttttctatt atcaaatttc tggctcacat ctttattcag
     4921 tctaccttct cctgccacga tatcttcaaa ttcaactttt ggaagtctac tggagtttgt
     4981 acttttatta agtctaacaa ctaaatcaat taacttacgc tcttcccaat cttccgtaaa
     5041 tcctgggaaa cggatttctg gtcgtgtatc attgctcatg tttgaacacc tccagcgcac
     5101 cttgtaacca catttcgttt tcaggtgttg tttgcaggga agagatagct tctaaaagtt
     5161 ctttttctag cacttgctgt tcttgtcgta tcttcttgat gtcatttccg acatgaacca
     5221 tatcaattgg ttcttcttct tcgaaagtat ctacatagcg tgggatattt aagttatagt
     5281 cattttcttt gatttcatca aaggttgcca aatgagcgta tttctcgaca tcttttcttt
     5341 cagcgtaggt ttcgaggatt ttttgaatgt tttcttccga tagtttattt tgatttttcc
     5401 ctttaacaaa ttcacgacta gcatcaatga ataatacatc tcgagtttga cgattcttct
     5461 tcaacacaat caccgtcgtt ggaatcgatg tcccaaagaa caagttcgca ggcataccga
     5521 tcacagcata aatactgcca tcttctagta atttttgacg gatgacccct tctgcagcgc
     5581 cacggaataa aactccgtgt ggtaagacga tggccatggt acctgtttcc ttcaaatgat
     5641 agaacccatg aagaagaaac gcaaaatctg ctttggattt tggcgctaat ttcccataac
     5701 gattaaaccg agaatcatct aagaaagtcg tatctgctga ccaattagcc gaataaggtg
     5761 ggttcattac tactgcatca aatgtgtaag gttcatctgt tggccagtcc ttattcaacg
     5821 tatccccatt gcggagattc atttcttcgg cgtcgacacc atgtaaaatc aagttcattt
     5881 ttgctagatt gtatgtcgtt gtatttagtt cttgcccatg atatttaacg ttatctgggt
     5941 gtgttaagta gttacgtacg tttaacatca atgaacctga acccatcgtt ggatcaaaaa
     6001 cactaaagaa tcgtcgttcc ttttgatcga gcgttacgat ttgcgccatc atatcagaaa
     6061 ccatatgagg tgtgtaaaat tctccggctt tcttccctgc ttctgaagca aattgactga
     6121 tcagaaactc atacgcatcc ccgatcacgt ccccatcgtg ttctaaaaca tctacatcat
     6181 tcaatttctt gatgacttcg gtaatcgtca catttcgttg ttgctcatct gttcctagtt
     6241 tttttgattg gagatccaca tcatcaaata acccattaaa ttgattgtag gtagatgcca
     6301 attgcacaaa ggctttgttt agatcgttca attggaacgt tgcttgtttt gcttgatccg
     6361 ctaagacgtt gaaaagatac tctggtgcaa ttgcatatcc caaaatatca acgattgtgg
     6421 caatcagatc ttcttttgat tcttcatctg aaagtaattc acgatacaac attgtttgtt
     6481 tgcttactgt gtcatattcc tctaaggatt catctgctaa aagaaccact tgttccaaca
     6541 atcgatcgga taaatattta tagaaaatca gtcccaataa ataattttta tattctgacg
     6601 catccatttt actacggaga ttatctgccg cactaaataa tttttgattt aattctgcac
     6661 ccaaaaagat acactcctat ctcttattta aagttgctat ataccctctt attttagcat
     6721 accccctttt ataaaaagca aaaaaaaaag aaagaaactt attcttgcta ataaactacg
     6781 cgaaa
//
LOCUS       DACDPS010000102         6582 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.102, whole genome shotgun sequence.
ACCESSION   DACDPS010000102 DACDPS010000000
VERSION     DACDPS010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.102"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            122..1753
                     /locus_tag="IWJ07_002421"
     CDS             122..1753
                     /locus_tag="IWJ07_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP9333957.1"
                     /translation="MVLIKDWQDSIEGLNKMQVQLPYFNVEAVKQEGQKHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELESGVVVCETFDEEVVEKAYHDYNNDILEIVMGEDGEL
                     KKRLLAVTADSLYCNPKNITDYQRVVKYFEDPTLQFTTFTITEKGYSLKDSKGQFFSV
                     VEADIKSGPTNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFMIAE
                     AWQKKGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSESVKMDLLKAGFEDLDIIHTA
                     KNTNIAAFTNTEKVHYLVIEDSFPNGRPKLEAAGVIMTDRETVDKADIMKVTTCLNPL
                     HTALAITGCLLGYKSIQAEMQDDDLVGLIKGIGYVEGLPVVESPGVIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGKNFVPSPDPLLVELQEKFSVLKLGEQDPAKIHCAVQEILENKEIFGLDLYQ
                     VEIGKKIEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            1756..2835
                     /gene="uxuA"
                     /locus_tag="IWJ07_002422"
     CDS             1756..2835
                     /gene="uxuA"
                     /locus_tag="IWJ07_002422"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP9333958.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            2909..4336
                     /gene="uxaC"
                     /locus_tag="IWJ07_002423"
     CDS             2909..4336
                     /gene="uxaC"
                     /locus_tag="IWJ07_002423"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP9333959.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            4420..5067
                     /gene="eda"
                     /locus_tag="IWJ07_002424"
     CDS             4420..5067
                     /gene="eda"
                     /locus_tag="IWJ07_002424"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP9333960.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSLENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            5187..6563
                     /locus_tag="IWJ07_002425"
     CDS             5187..6563
                     /locus_tag="IWJ07_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9333961.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKTSNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENC
                     ELLKQKHK"
BASE COUNT         2246 a          881 c         1353 g         2102 t
ORIGIN      
        1 acaataattc tgacatggta gaaaaaagaa tatttttagt tttttcttat tgacagcgat
       61 tacattcgat tttatactac aattgtaacg gaaactaaca tatcagtatg gaggggtaaa
      121 tatggttttg attaaagact ggcaagattc tatcgaagga ttgaacaaaa tgcaagttca
      181 actaccgtat tttaatgtcg aagcggtcaa acaagaaggt cagaaacatc cagtgtggct
      241 tcattttggt ggaggaaatc tttatcgagg atttcatgca gaaattgctc aatgcttagc
      301 agatcaaaat gaattagaat ccggagtagt tgtgtgtgag acatttgatg aagaagtagt
      361 agaaaaagct tatcatgact acaacaatga tattttggag attgttatgg gtgaagatgg
      421 tgaactcaaa aaaagacttt tagcagtaac agctgatagt ttatattgca atccaaagaa
      481 tataacggac tatcaaagag tggttaaata ttttgaagat cctacattac agtttacaac
      541 gtttactatt actgaaaaag gatacagttt aaaagattct aaaggacaat ttttttcggt
      601 agtggaagca gatataaaat ctggacctac caatcctcaa catacgatga gtattgtgac
      661 atccttgttg tatagcaggt ttaatgctgg agagttaccg attgcgatgg tttctactga
      721 taatttctca cagaatggtc aacgttttca agatagcatt tttatgattg cagaagcttg
      781 gcaaaaaaaa ggatacgttt ctgaaaaatt tattgcttat ttaaaggata aagaaaaagt
      841 gacttttcct tggagtatga ttgatagaat tacgccaaat ccttcggaaa gtgtcaaaat
      901 ggatttattg aaggcgggtt tcgaagactt agatataatt catactgcga aaaataccaa
      961 tatcgctgct tttaccaata ctgaaaaagt ccattatcta gtcattgaag atagttttcc
     1021 aaatggtcga cctaaactgg aagcggctgg tgtgattatg acagatagag aaacagttga
     1081 taaagcagat atcatgaagg ttacgacatg tttaaatccg ctccatacgg cattagcaat
     1141 aacaggttgt ttgttggggt ataaatcaat tcaggcagaa atgcaagatg atgatttggt
     1201 tggcttaatt aaagggatag gttatgtaga aggcttacca gtagttgaaa gtccaggcgt
     1261 tattgatcca aaacaattta ttgatgaagt aatcaataaa cgtttgccta atccctatat
     1321 tccagataca ccgcaacgaa ttgcaacaga tacttctcaa aaagtagcga ttcgctatgg
     1381 tgaaacaatt aaaaagtatg tcgaaaaaga cggaaatgca gaaaatctaa catttattcc
     1441 tatcgctatt gctgcttggt tacgttattt attggcagtc gatgatcaag gaaaaaattt
     1501 tgtgccaagt cctgatcctt tattggtaga gttgcaggaa aaattctcag tattgaaatt
     1561 aggtgaacaa gatcctgcaa agattcattg tgcggtacag gaaatcttag aaaataagga
     1621 aatatttggt ttagatttat atcaagttga gataggaaag aaaattgaaa aaatcttttg
     1681 cgaaatgcta gtaggtcctc aagcagttcg taaaacaatc aataaatata taacagaagt
     1741 tggaggaaag taactatgga aatgactttt agatggtatg gggcaaatga agaaaaaatc
     1801 tcactacaag aaattcgtca gatcccagca atgaaaggaa ttgttggaac attaatggat
     1861 ataccagccg gagaggtttg gccgagagaa cgaattaaat ctttaaaaga tgaagtcgaa
     1921 gccaatggac ttaccttgaa agttattgag agtgttaatg ttcatgatga tatcaaaatt
     1981 ggtttaccaa cacgagatca atacattgag aattataaac agacgattcg caatttggca
     2041 gaatttggga ttgaggttat ctgttataat tttatgccag tatttgattg ggtaaaatcg
     2101 gatttggatt atcgtttaga tgatgggtct tctacgttag cctatatttc aaaaaatatc
     2161 cctactgatc cggaggaaat tattagaaaa gtagaaaatt ctactaataa ttttgaattg
     2221 ccgggttggg aacctcatcg gttatcacaa attaagcaat tattagaagc ctataaagaa
     2281 gttgatgaag agaaattacg agagaacttt gcatattttc tcaaagcaat tatcccgaca
     2341 tgtgaagaag ttgggattaa aatggcagtt cacccagatg atccaccata ttccattttc
     2401 ggattacctc gtattgttaa aaatcgtgac gatttagatt ggttgtgtaa tgtagtggat
     2461 tctccttcta atggtattac attatgtact ggaagtattg cagaacgacc agataatgat
     2521 gtctatgaaa ttctatctga attttgtcaa cgggatcgga ttccttttgc acatgttcgt
     2581 aatatcaaat atttaagtga caatggactc gatttttacg aagcgccgca taaatcagaa
     2641 tatggttctc tagatatgta taagatatta aaaacaatgt acgataatgg atttaatggc
     2701 tatattcgtc cagatcatgg tcggatgatt tggggagaaa ccggtagacc tggttatgga
     2761 ttatatgacc gagctctagg tgcagtttat ttaaatggta tttgggaagc gcttgaaaag
     2821 ggggcgagta aataattata taagggagtt tatttagagg aaaaattttc tcttctaaaa
     2881 actaacggaa ggaaaggagt gtttaaatat gtttttagat aagaactttt tattagagaa
     2941 tgaaatagct aaaagacttt ttcataaatt tgcaaaacac caaccaatta tcgactatca
     3001 ttgtcattta gacccagcat atttgctaga aaataagcaa tacaagaatt tgactcaggt
     3061 atggttaaat gataaaggag ctggagatca ttataaatgg cgattgatgc gagctaatgg
     3121 tatagaagaa gagttgatca gtggtccagg tgatgattat gaaaaatttt tagcttttgt
     3181 aaaaacagtg gagaaggcgc caggtaatcc tatttttgaa tggtctcatt tggaattaca
     3241 acgttacttt gatattgatt tgacaatttc tgagaagaat gcaccagaaa tttggcgacg
     3301 tgctaacgaa aaaattcaaa cggattccta tcgcccgaaa gaattgttgg agatgatgga
     3361 tgtgaaagcc atctgtacaa cggatgatcc tacagatgac ttgtggtatc atactgagct
     3421 ttctaaacag gataattctt tcaaggtatt acctactttc cgtccagata aggtctggag
     3481 tatcaaatca tcaggctttt cgaactatat tttaaaactt agtcaaacag cggctatacc
     3541 aatcaaagac tttaatgggt tgaagcaagc attgagccaa cgagtggatc attttcatca
     3601 aattggctgt cgattagcag atcacggttt gaatacttat ttttatgaaa tagcagatga
     3661 aaaaatactc gacaatctgt taataaaagc tttagcagga aaaacggatt tccatttatc
     3721 agaaattgca caatttgtta caggtattca attacatttg atgtctgagt atacaagata
     3781 tggttggacg atgcaaatgc atttaaatgc tttaagaaat aattcaagta tattaaagga
     3841 acaaattgga gtagatgtcg gtggtgattc gatgggggat caagctagtt tgacacaaaa
     3901 tgttgtagca ttgttcgata ccgctgaaaa gagtgggtgc ttaccaaaaa caattttata
     3961 ttcattaaat ccaactgatt ggcttccact agcaacagca atgcaaagct tccaaggggg
     4021 tatgattcaa aaaatccaat tgggatgtgc ttggtggttc aatgatacag ctgaaggaat
     4081 gcgtcaacag ttgactacga tggcacaaca aagtttgttg tcgaatttta caggtatgtt
     4141 gactgattct cgaagttttc taagctatcc tcgccatgag tattttcgtc gagtattatg
     4201 tcaattacta agcgagtggg ttaaagcagg ccgaattcca gatgatgaaa tgtatcttag
     4261 tcagatagtg gagaatattt gttatcgtaa tgcgtataat tattttaatt tttttgaaat
     4321 cggaagtaga aaataatact tgaaataaaa gcttaaattt attctttgta agttagtaca
     4381 gctagacaaa gtatgtatcc ataaaggaag gagatagata tgaaaaaagt agaaatttta
     4441 agtaagttag aagcggctgg agtcattgcg gttgtacgtg ggaaaaataa ggatgaggcc
     4501 attaaagcaa taaattcaat tattgcgggt ggaattaaag ggattgaact aacttttaca
     4561 gttcctaatg ctgaagaaat aatcaaagaa attgctaaaa aatatgctga tcaaaaagat
     4621 gtaataatcg gagccggtac ggttttagat gcgtttactg ctcgtatcgc aattatggct
     4681 ggggcagaat atatcgttag tccatctttt gatcgacaaa cagctgagat ttgtaattta
     4741 tatcaaattc cttatttacc aggttgtatg acaattactg agataaagga ggcacttact
     4801 gctggcgtag atatcattaa actattccca ggttctgctt ttggtccatc cattgtaaaa
     4861 gcctatcaag ctccattacc acacgtaaat ataatgccta ctggcggagt aagtctagag
     4921 aacttagaag attggtttga agtaggagtt gttgcagttg gtgtaggtgg gaatttattg
     4981 gcttctgttg aaaaaggtga ttttgaagaa gtaagtcgag tagctagtaa atatatagat
     5041 aagttttatg agataaagaa ttattaggag aaaaaacaaa taatactctg aaatgtaaaa
     5101 aataaatact gactctttac gcttcagagt atagtacgta ctttcatcag aatgaaaacg
     5161 cttaacaaca gaagggagag aattgtatgg aaagaaaatt tggtataaga gacaaaatag
     5221 gatatatgtt tggagatttt ggaaacgatt ttactttcat ttttgctagt tcttttttaa
     5281 tgatctttta tacaaaagta ttggggataa gtggagctgc cgttggaaca ttatttttag
     5341 tagcaagatt tatcgatgca gttactgatg taactatggg gcgtattgtt gatagatcga
     5401 aagcaactaa gcagggtaag tttaaaccgt ggattttaag aatgagtggt ccagtcgctt
     5461 tagcaagttt tttaatgtat caaacttcta tgatttcagc ttctatgagc gttaaggtag
     5521 tatacatgta tgttacgtat atattatggg ggagtatttt ttacacatct ataaatattc
     5581 cttatggatc aatggcatca gctattactt ctattacaga agaaagaaca atgctatcga
     5641 cattccgaaa tgttggagct actttagcaa gtttggtcat tggtgtatta acaccattat
     5701 ttatttatac aagagatgat tcaggcgctc aaatagttcg tggtggatca acttttacaa
     5761 ttgttgcggg aatattttcg atatgtgcac ttatttgtta tttaatatgt tataaactaa
     5821 ctactgaacg agtaagaatt gaccaatctc aatacaaagt ttcattgtta aaatctttaa
     5881 aagggttagt taaaaatagg gcattacttt ctataattgg tgcggctatt ttcttactcc
     5941 ttgctcaatt attaattatg agtatgaata attatgtatt tcctgattac tataatgatg
     6001 cacagggaat agttttaatg aattttatca atcctatttt ggttttaata gttgtttcac
     6061 ctttaacttt gtttttaagt aaaaaatatg gaaaaaaaga actggcatca gtaggaatgt
     6121 tcttttcagc aatagtatat ttgatcttat ttttgataaa aactagtaat atgtatgtat
     6181 tcttaattct tagttcggtt ggctatatgg gtttgggtgt gtttaatact gttatttggg
     6241 caaatattac tgatgttatt gatgatcaag aagtaaagtc tgatcaaaga gaagatggga
     6301 ccgtatatgc ggtatattct tttgctagaa aaataggtca agctctagct ggaggcgtcg
     6361 gaggttggac tctttctatt ataggctatg actctttagc aaatgtacaa acgggagatg
     6421 ttttagaaaa gttatataac gcttcaacat taattccatc aatttgtttt ctgattgtag
     6481 gattaatttt atttttcctt tatccattat ctaaagataa agtcttagaa aattgcgagc
     6541 ttttaaaaca aaaacataaa taggagagaa atatgaataa tt
//
LOCUS       DACDPS010000103         6445 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.103, whole genome shotgun sequence.
ACCESSION   DACDPS010000103 DACDPS010000000
VERSION     DACDPS010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.103"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            294..650
                     /locus_tag="IWJ07_002426"
     CDS             294..650
                     /locus_tag="IWJ07_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333962.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            653..1810
                     /locus_tag="IWJ07_002427"
     CDS             653..1810
                     /locus_tag="IWJ07_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333963.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(1836..2276)
                     /locus_tag="IWJ07_002428"
     CDS             complement(1836..2276)
                     /locus_tag="IWJ07_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP9333964.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            2436..2942
                     /locus_tag="IWJ07_002429"
     CDS             2436..2942
                     /locus_tag="IWJ07_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9333965.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            3110..5023
                     /locus_tag="IWJ07_002430"
     CDS             3110..5023
                     /locus_tag="IWJ07_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP9333966.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            5028..6029
                     /locus_tag="IWJ07_002431"
     CDS             5028..6029
                     /locus_tag="IWJ07_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9333967.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(6231..6437)
                     /locus_tag="IWJ07_002432"
     CDS             complement(6231..6437)
                     /locus_tag="IWJ07_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAP9333968.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
BASE COUNT         2366 a          971 c         1286 g         1822 t
ORIGIN      
        1 taaaagcaac agatctaaaa ttgaataaaa cgatagattt ttgagtgaat atttcattcg
       61 agagtgctgt attgcctggc gaagttgtag ctttgccaga tctatttttg tttactaaca
      121 tatttgagaa acaggaaata acaggaacac aaatggaaaa taagagcaag aataattgac
      181 aatgcacact gcgcatggta gtatctctga aaaatcagta acgaatctat ttgtacgtta
      241 ttcatgtagc tgtcggaata agtctaaata atagataaaa aggagtatgt actttgtttg
      301 aagaatttat tgatatcaat gaaaggcaag tatatcaatt tttgaactat tgttatgaaa
      361 gagacgagaa attatatgtg gtgaaggata tcgctttaga tctaaactac accctggcta
      421 agatgaattc ggtgattcaa caagcagaat cgttttgcga aaggtatcca gaatataaat
      481 tatcttttct ttcagaaaac aagatgataa aagtcgagtt ttcttctcag tttttactga
      541 gtaaagttta ttctatactt ttagagggaa cgattggtta tattttactg gattcacttt
      601 acaaaggtac ttatcaatcc ttagaaaatc ttagtcaaaa aattatataa gtttgcgtac
      661 gattcaaaga aaattaagag aacttaatac tattttgaaa aactatcatt tatcttgcag
      721 cttgaaaaaa ggtaatccga tagaaggaga agaataccgt atccgatact tttttcactt
      781 gatgtattgg cagtcattcg acgaaaatca ttctgatata ttatcaagta taaaaaataa
      841 acgtcaacta ttagaaaaat ttgcgaaaca tgcgccatat acgcgtagta tagatagaaa
      901 gaaatggatc agcctactaa gaatttcgtt gcaacgaatg aaacaaggcc actcgattac
      961 gtatgtccca gaagagatga acaaatttgt gcatccattg ataacaaaag aagagtttac
     1021 gcattacata ctagagcctt tttttgaagc aaacttctgg tcgatgaatc acatcactcc
     1081 aacagagcaa gcttaccttt attttatgtt tggtgtaatg aacacctatt tggaagaaga
     1141 tttgttggat actccagata aagtacaaga aagttggggc tggattgaat cgtctattgt
     1201 agaaaatttt gaaaaaaact tcaagatttg ttttacaact tcagaaaaaa attatctata
     1261 tgtcaatttg gcaatgatcc atttgtattc aagggttttt gggacaaaaa agaaggtgga
     1321 tgcttttgga aaatcagcag aagacagaga ttttcagcat atatttccag tgatgtatcc
     1381 aatcatgcaa cagtttttta gtggggtagc tcgcagaatt ccagagctta atacttatta
     1441 tgaaaagaac agacggttga tttttcaata ctgtatgtta gtcagggata tttttataaa
     1501 acacgaacaa cctgtcatat tctatattca atcaaagtat ggtaaaacac aggaattatg
     1561 ggcaaagaaa aggatattat ctatgactga acgcccaata aaatatagtg catccgctga
     1621 tcagttacct gatattgtca ttagcgatta tcctatcgac aaaaaggcat atgatcaaac
     1681 gaatacacag atattttact ggaatggaca acctacaaaa aatgattggg cgcgtctgaa
     1741 agctgaaatc ataaaaatca ggaatgagaa aaatagatcc atattggaaa aattggattt
     1801 cgttaattaa gaaaagcaga ttgtttatgc caaaattaag acattgcaaa aagattattt
     1861 tctttaaaat cgacaagatg accagaaagc aagagttcgt tgagctcttg ctttctttta
     1921 gttttagaaa ggaacatcct gattttatct tcgctaaatc cagtcaaaag ttttgcagat
     1981 tggtcgtaga taatcggttt tttcaataaa gaaggggatg tacgaatctc aatatatagt
     2041 tcactcaaac tgagttcatc tattctgtga acaagatttt tataactaat agaatgagta
     2101 gagagcattt cggtcacgcc gttgtttgtc aattttagta agtggataaa ttcatttttg
     2161 gttagtggtt gacgaattaa gttctgttcg ataaacggta tatcatggac gataagccaa
     2221 cgttttgcgc gtcttgaaga cgagcagcca gaacctgtat acaatttaat catcatacat
     2281 tcctccaata gctaagtatt tatgaaataa taacatgaag aaatagtcga aaagtgccat
     2341 ttagaggtgt aaaataagaa aatgttgtca cgtataatgg aactagctag ataagaaata
     2401 ctattaaatc aagaaaaaag aaagaaggca caagtatgtc aaacacagcc gacgcaatag
     2461 tagtgatcga tttacaaaaa ggggtatgtt ttgattcttc gccgatacac gaattagaaa
     2521 cagtgacagc attagtgaat aaaagaatca aaacatacga aacgattggg aaaccagtta
     2581 ttttcgttca acacgaagat gctcaattag tgccacaaac aaaaccatgg gagctattgt
     2641 cggaattatc tattcctgag cacagttatt ttgtgagaaa aacccatgcg aattcatttt
     2701 ataagactaa tttgagtgtt ttattaactg aattggactg tcattcgatc gaattatgtg
     2761 gggcacaagc agagtattgt atagatacga ctgtgaagtt tgctcatgga ttaggatacc
     2821 agcttatgat gtgtagagga gcaacgacaa cttatgataa tccttttatg aaagcaaagg
     2881 aaacaatcgc tttttacgag aaaatttggg ataaacgatt tctgacattt ttggagcaat
     2941 aagttgtttc ttcgtttaaa aaaagcagaa tggatactgg tttatccatt ctgctttatt
     3001 acgtaaaatg agaaaataat cctgttctat ttttatggga taaaccattt attttttgta
     3061 taaaaaatgt ttgcgtttac atataattct cctagaaaca taaaaaggag tgaatagctt
     3121 gagaacaaaa caagtattaa agggagcgtt ggtcatctta tttgctgtaa gtacaagcgt
     3181 cccgcaatgg acagtatatg gagagagcat ttcttccgaa tcggaatctc gggaggtaga
     3241 aaccacagaa actactgatg ctggaaatga aacgaaatcg agtacttcac aaacagaaac
     3301 aaatactgaa acaacggttg agacatctgt tactagcaca gatcaagaaa cgaccgctac
     3361 cacagataac caacaaacag aagaagcaac agctacaaaa atgtcaaacg acgaatcctc
     3421 ttctgctaaa gtaagtgaca aagagaatgg gacagaaaat gatgatatcg gtaaaggtga
     3481 aaatttcaat ggtaaagaat atttgaatta cttcggtaaa cagatgacag aaatacttaa
     3541 tggaccaaca gaagacatca agtcaaaatt aatagagtta ttagtcactg agcttgctgt
     3601 taatgattat ggtgtagata aagagattat caaacatctg attgctactt atctattgta
     3661 cgatatcgct tttgcaaaaa acagtgtgtt gatgtcgatg cttggaggta cagatgatgg
     3721 gacaacgcct acagaaaaca caggcttcta tccaaataca aaacaacttc atcaaactgt
     3781 actaaacaaa caagatggca aaatgatgga tgtatatgca tattatgtcg atcaaggttc
     3841 agataaaaca gtcatgatcc atggcggatt tcgagggaat tggaataacg ggatcgttac
     3901 agaagaatac aatgattttt ataaggccgg gtataattta ttatttgttg attccagagc
     3961 gactggtaat agcggaggcg attatgtgac ctatggacaa tatgagtcag atgacgtcct
     4021 ttactggata aatcaagaag ttcgagagcg gccatctcaa aagattttac tttatggtgg
     4081 ttcgatggga gcagctacga tgatgtctgt attagcgaaa gatattcctg tcaatgtcaa
     4141 aggaattatt gaaaattgcg gatttgcatc aattgatgaa caactacgtt ttacgtacag
     4201 ccagacagta gcccccgcac taccggatgc cataaaaaat cagttagaca tcattgggga
     4261 tcaagagcat gaagatttat ttatgggcct tttgaaacaa tactattttg atcaggagat
     4321 gcatttggat acaaaagctg ctctacctac gattggaatg tcagattcat tgcctaaatt
     4381 gattatccat ggaacagcag atgatgtagt acctgtgtct aacgcgcaaa aattatatga
     4441 gttatcagga gggtataaag atcttttatt agtagaagga gcagggcatg gaaaggcaca
     4501 ggaagttgac catgctgcct acacgaaaca tgtaacggac tttttgaaag tagtgttcca
     4561 tgatcagata aacgtaaagt atgtggatga aaacaataaa agtctactca aagaccagga
     4621 cgaaatccag ctttacggag cttatggaga aaattatatg acagagcaaa agacattcga
     4681 aggatacgaa ttagcaaatg ttgaaggacc gactgaaggg atttttaatg agacgatacc
     4741 aactattatt ttctattaca aaaaaatccc agtagctcca ccaaaaaagc aagatccgac
     4801 agagaatgca gacaataagg gaaagaatga acctcgagag gatcaatcaa gtgatgatac
     4861 gcaaccgatt cagatgacag tgaaagtaca aaaaaagaca gatgcaaaaa aaagaatgtt
     4921 gccgaaaact ggagaaaaga aacattcgct tctcatttat tcaggtggat ttttatctgt
     4981 tagcacgatt atcacatgga tttggcgaag aaagagaggg taaatgaatg aaatggttca
     5041 agcgaatagt gattgcagta ccagttatca tcgtagttat tttagtcact gtatttttga
     5101 ttaatcaaat cacaccaaag ccgattagct tgcttgttag aaaacaattt gacacaagtg
     5161 aaaaagaaca agtatatgcc cgaccagatg atttccagca aaaaatcgaa caaatcgata
     5221 taaaaaaaga aatatcctat ccttcaaagt acaaaaataa taatatggat atctacacac
     5281 cgaaagtgga aaagaaaaaa ttaccgattc ttttctggat gcatggaggc gcatatgtag
     5341 gaggggataa aaatgattgt cgtgattatt tggaatatct atgttcagac acgcagcaaa
     5401 tcatcgtaaa tatagattat gaacgatcac cggaagcaaa acatcctaca ccagttatcc
     5461 aattgaacga agccattcaa gcagctaaaa aagagataaa agatcaagca gattggtcga
     5521 atatttcgat tggtggaaat tcggcaggtg cacaaattag tggagaatat ttgttagcac
     5581 ttcaaaatga agaaattaaa aaagctgatc atcttgatcc gactttggag aacaagcaga
     5641 ttacgaagtt tatttctttg tctggtctgt tggatccttc tgcacttact caagtaagtg
     5701 ataaaatctc tagctttctt tatgaaaaat gtggatgggc ttactttgat aataaggact
     5761 ttgaacaatc agagaaggtc aaaagtttag ccttggtcag acatgcggat cgttggacgc
     5821 aaaacacatt cttaacggat ggaaatacaa atacgtttac gaaacaaatg gaagaagtaa
     5881 cttctgtatt tgagaaagcg ggggtgaaag tcacaaaagt agattatgcg gagaaaaatg
     5941 ctgttttgaa ccatgaatat cagtttgatt tcagcaataa acaagcgcaa gaaacatatc
     6001 aaaagttagt aatgtttttc aaggagtaaa gtcttatgat gcaaaagagg ttgtaatctg
     6061 tcacttagct ccaagtcaca aggagcaatt tcaataaaca agcttttcat ggtttatgaa
     6121 tattgtaact tgtgactgag gagttagaga tggcaagggg caccttttat tcgaaatttg
     6181 ggaatatcac acgattattg tcacgatctc ttttacgtaa attttataag tcagatttgt
     6241 tgttttgatc gtttaaaaaa agcaacaaat ccgaagcctg cggaggcaag aagggacaaa
     6301 aagccgagtg gtaagcaaaa gaatgctggt tccactaata ttccgttgtt attgatataa
     6361 ggctgggaac aaagatgaat agcaaataaa atcaatgcac tagcgaaaaa aagcaaagaa
     6421 atatggatag ctttcataag cagca
//
LOCUS       DACDPS010000104         6326 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.104, whole genome shotgun sequence.
ACCESSION   DACDPS010000104 DACDPS010000000
VERSION     DACDPS010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.104"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(99..1229)
                     /locus_tag="IWJ07_002433"
     CDS             complement(99..1229)
                     /locus_tag="IWJ07_002433"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAP9333969.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1878..3080)
                     /locus_tag="IWJ07_002434"
     CDS             complement(1878..3080)
                     /locus_tag="IWJ07_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9333970.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="IWJ07_002435"
     CDS             complement(3140..3343)
                     /locus_tag="IWJ07_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP9333971.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="IWJ07_002436"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="IWJ07_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4880..5692
                     /locus_tag="IWJ07_002437"
     CDS             4880..5692
                     /locus_tag="IWJ07_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9333972.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1883 a         1299 c          997 g         2147 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaa
//
LOCUS       DACDPS010000105         6245 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.105, whole genome shotgun sequence.
ACCESSION   DACDPS010000105 DACDPS010000000
VERSION     DACDPS010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.105"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            620..1894
                     /locus_tag="IWJ07_002438"
     CDS             620..1894
                     /locus_tag="IWJ07_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9333973.1"
                     /translation="MSKVNVFKNQSYLQSSATLLLFFASWGVWWSFFQLWLTSESNGL
                     GLSGSAVGTVFSANSLVTLILMFIYGTLQDKLYIKRNLLIFASVLATLVGPFFIWIYG
                     PLLDNNFNLGIIMGALFLSAGYLASVGVFEAVSERFSRLFGFEYGQARAWGSFGYALV
                     ALLAGFLFVKNPHLNFWAGSFFGSLLLLNLLFWNPKVEREANQNFNQEQAESNSIPSL
                     KEMFDLMKLPQLWTIIIFIVFTWTFYTVFDQQMFPGFYTGLFSTSANGEKIYGTLNAI
                     QVFCEALMMGIVPIIMRKLGVRNTLLLGVTIMCVRIGLCGFASTPLSVSCIKMLHALE
                     VPLFTLPMFRYFTLHFDTKLSATLYMIGFQIAAQIGQVILSTPLGILRDNVGYQPTFK
                     IISLIVLLAGIYAFFILKQDDRDVQGDPFIRG"
     gene            1948..3375
                     /locus_tag="IWJ07_002439"
     CDS             1948..3375
                     /locus_tag="IWJ07_002439"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAP9333974.1"
                     /translation="MTKRAIELTNTRYRLGYHVSAPSGWINDPNGFCYFDGYYHVFYQ
                     HHPYSAEWGPMHWAHARSKDLVHWESLPLALTPGDQEDEGGCFSGSAIEKNGVLYLFY
                     TGHHYYGDNDPDHFWQNQNMAYSTDGIHFTKYEKNPVIAKAPEDNTHHFRDPKVWKHD
                     EKYYMVLGSQEKDGLGRAIVYQSLDLLNWDYIGPISKAKEVLTEGFMWECPDFFELNG
                     KSILLLSPQGIEANGKDYLNLYETGYFIGDYDYETAKFIRGNFYELDKGHDFYATQTT
                     LSPDGRRIVMAWMDMWESPMPEKVDGWAGALTLPRELELIGDHLYMKPVRELEQLRVG
                     SGVETKVNLAGEIIIAQDASFKEALIDVLLTKSKNQEIKFSLKTKDEELINIFYSKDK
                     NECTLTRNGKNDPRYGTIQMCDRLSLRVFIDKSSAEIFINDGELVFTERYYTENQPSM
                     HLSVSEEAFVSSTIYHLKKDAISYE"
     gene            3388..4260
                     /locus_tag="IWJ07_002440"
     CDS             3388..4260
                     /locus_tag="IWJ07_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9333975.1"
                     /translation="MLIGSIEAGGTKFVCAVGDEDYRVQDQIICPTTTPRETLKKTVD
                     YFKQFDINALGIASFGPIEIRKNSPKSGYITSTPKVGWRNVNVLGYLQDELNVPMSWT
                     TDVNGSAYGEYIMSTLSNEKINSLVYYTVGTGVGGGAVVDGKLLGSFGHSEMGHTFVK
                     RHPDDIDFKGICPYHGDCLEGLVAGPTFEARFGKKGEDVSLTDPIWDVMAYYLAQAVI
                     QTTLIIRPDKIVFGGSVVNEKLLEKIRVNVNELLDDYVEIPPLSKYITRPLIKNNGSA
                     TLGNFALGLNQLQE"
     gene            4355..5350
                     /locus_tag="IWJ07_002441"
     CDS             4355..5350
                     /locus_tag="IWJ07_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9333976.1"
                     /translation="MRPKLEDVAKRANVSKTTVSRVLNNRGYLSQKTIDNVYKAIEEL
                     NYQPNVVARQLFQKKTNIVGLLFPTVANPFFSELVEALEKKLYEIGYKVLIGNSMNNK
                     EKETNYLNQLLSDQVDGLIVGTHNQGIQEYKYQNLPIVAIDRVMNEDIPVVESDNYNG
                     GKLATKLLIAQGAKNIIHTNGPIDLQTPANRRRLAYEDTMKAYQLIPRTVTLDFNISY
                     VKKKQIFFQMFEDYPKIDGIFASNDIDAALILQVAKEKGLNVPADLLVVGYDGTLMTR
                     SILPDLTTVIQPINDIADTAVAILMKRINKEETKKEYILPVTLWMGKTSVKKKDN"
     gene            complement(5955..6062)
                     /locus_tag="IWJ07_002442"
                     /pseudo
     CDS             complement(5955..6062)
                     /locus_tag="IWJ07_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302409.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2167 a          859 c         1170 g         2049 t
ORIGIN      
        1 ttctagttaa ggttcctgaa taagtcgcaa tccaattatt tccagctgat ttataacttt
       61 ttaaatataa ttttccataa taccaataac cattactttt ttctgtccaa tttcgttatt
      121 gagaagatct agaaggccag ccaactttat caaaaatcat cattcctgtc actcgtatcg
      181 ttttagaagc tgctcgagta agtaatggag aaactgttaa ataattttaa gtaacagtta
      241 agtcgttagt tgggttatca atggctattc cgtccttatc tgaacctcga aacccaatca
      301 tagaaaaagt tttttgaaca agttttaaca gataaatcaa gttatatact agaaaattcg
      361 taaaatatat ttttaatgtc ataatcgttt gatttttctg gattgacatt tatttataaa
      421 aaatagtgcc attaaaaaag aaaagtgaca caccaataaa agtcatatcc tgtgacatta
      481 tatggcatta tccaaataga ataaccggtt gacatgtaag cggattcctt ttataatttt
      541 aggagtataa gtccttttct aaaaatatta accggttatc atgaaagggg atacaaatat
      601 atcgttggag gataaaaata tgtcaaaggt taacgtattt aaaaatcaat cctatttaca
      661 aagttcagct acattattac tattttttgc ttcttggggt gtttggtggt cattttttca
      721 actttggcta acatctgaat caaatggttt agggttatct ggcagtgctg taggaacagt
      781 attctcggca aattcgttag ttaccttaat tttgatgttt atttatggaa cattacaaga
      841 taaattgtat attaaacgaa atttattaat ttttgcttct gtattagcga cacttgttgg
      901 accatttttt atatggatat acgggccatt gctagataac aattttaatt taggcattat
      961 tatgggagca ctatttttgt cagctggata tttagcttct gtaggagttt ttgaagctgt
     1021 gtcagaaagg tttagtcgtt tatttggctt tgaatatgga caagcaaggg cgtggggatc
     1081 atttggttat gccttggtag cgcttttggc aggattttta tttgtaaaaa atcctcattt
     1141 aaacttttgg gcgggatctt tctttggttc tttactattg ttaaatttat tattttggaa
     1201 ccctaaagtt gaacgggaag caaatcaaaa ttttaatcaa gaacaagctg aatcaaatag
     1261 tattccttct ttaaaagaaa tgtttgatct aatgaaactg cctcaattat ggacgataat
     1321 catctttatt gtttttacat ggacatttta tacggtattc gatcaacaaa tgtttccggg
     1381 attttatact ggtttgtttt caacatcagc taatggtgaa aaaatatatg ggacattgaa
     1441 tgctattcaa gtattttgtg aagcgttaat gatgggaatt gttccaatca ttatgagaaa
     1501 attaggggtt cgaaatactt tgttattagg tgtaaccatt atgtgtgtac gaattggatt
     1561 gtgcgggttt gcctcgacac cattatctgt ttcatgcata aaaatgttgc atgctttaga
     1621 agtaccatta tttacattac caatgtttcg ctattttaca cttcattttg atacaaagct
     1681 atcagcaacc ctctatatga taggatttca gatagctgct caaattgggc aagtgatttt
     1741 atcaacacca ttgggaatat taagagacaa cgttggctat caaccaacat ttaaaattat
     1801 ttctcttatt gtattactag caggcatata tgcattcttt attcttaaac aagatgatag
     1861 agatgttcaa ggggatccat ttattcgagg ataatggaaa ccaacagaat aaaaaacaat
     1921 tttatctaga aagtgagcga atatattatg acaaaaagag ctatagaatt aacaaataca
     1981 cgttatcgtt tgggatatca tgtgtctgca ccatcaggat ggattaatga tccaaatgga
     2041 ttttgctatt ttgatgggta ttatcatgtg ttttatcaac atcatccata tagtgctgaa
     2101 tggggaccaa tgcactgggc acatgctaga agtaaggatt tagttcattg ggaatcttta
     2161 ccacttgctt tgactcctgg agatcaagaa gatgaaggtg gttgtttttc aggaagcgct
     2221 attgaaaaaa atggtgtgtt gtatctgttc tatacaggac atcactatta tggagacaat
     2281 gatccagatc atttttggca aaatcaaaac atggcgtata gtacggatgg cattcacttt
     2341 actaaatatg aaaaaaatcc tgtaatagca aaagcaccag aagataacac acatcatttc
     2401 agagatccaa aagtatggaa acacgatgaa aaatattaca tggttttggg tagtcaagaa
     2461 aaagatggat taggtcgtgc tattgtttat cagtcgttag atttattaaa ttgggattac
     2521 attggaccaa tttctaaagc gaaggaagta cttacagaag gatttatgtg ggaatgccca
     2581 gacttttttg aattaaatgg aaaatctatt cttttattat cccctcaggg aattgaagca
     2641 aatggaaaag attatttaaa tctatatgaa acaggctact ttatcggtga ttatgattat
     2701 gagacagcaa aattcattag aggaaatttt tatgaattag ataagggaca cgatttctat
     2761 gcaacacaaa caactttatc tccagatggt agacgtattg ttatggcttg gatggatatg
     2821 tgggaaagcc ctatgcctga aaaagtagac ggatgggcag gagcattaac gcttcctcga
     2881 gaattagagt taattggaga tcatttgtat atgaaacctg tgagagaatt agaacaactt
     2941 cgagttggca gcggcgttga aacaaaagtt aatttggcag gagaaataat aatcgctcaa
     3001 gatgcttcat tcaaagaagc tctaattgat gtactattga ctaaatcaaa aaatcaagaa
     3061 atcaaatttt cattaaaaac aaaagatgaa gagttaatta atatttttta ttctaaggat
     3121 aaaaatgaat gtaccttaac tcgtaacggc aaaaatgatc caaggtatgg aaccattcag
     3181 atgtgtgata gattatcact tcgagtgttt atcgataaaa gttcggcaga gatttttatt
     3241 aatgatggcg agcttgtttt tacagagcgt tattatacag aaaatcaacc aagtatgcat
     3301 ttgagtgtta gtgaagaagc ttttgtctcg agtacaattt atcatctaaa aaaagatgca
     3361 atttcttacg agtaaggagg ttttatgatg ttaattggta gcatagaagc aggtgggact
     3421 aaatttgtat gtgccgtggg agacgaggat taccgagtac aagaccaaat aatttgtcca
     3481 acgaccaccc ccagggaaac attgaaaaaa actgttgact attttaaaca gtttgatata
     3541 aatgcattag gtattgcatc atttggtcca atagaaattc gaaaaaattc accaaaatca
     3601 ggatatatta cgtctacacc aaaggtggga tggcgaaatg ttaatgtgtt aggttattta
     3661 caagatgagc tgaatgttcc aatgtcttgg acaacggatg tgaacggatc agcttatgga
     3721 gagtacatca tgtccacttt atcgaatgaa aaaataaatt cattagttta ttacaccgtt
     3781 ggtactggcg ttggtggtgg agctgttgtt gacggaaaac tattaggatc gtttggtcat
     3841 tcagaaatgg gccatacgtt tgtaaaacgc catcctgatg atattgattt taaaggaatt
     3901 tgtccttatc atggtgattg tttagaaggc ttagttgcgg gaccaacgtt tgaagcacgt
     3961 tttggaaaaa agggtgaaga cgtttcttta acggatccta tttgggatgt aatggcctat
     4021 tatctagcac aagctgtgat tcaaacaaca ttgattatac gaccggataa aatagtattt
     4081 ggtggaagcg tagtaaatga aaaattgtta gaaaaaattc gtgttaatgt taatgaatta
     4141 ttggatgatt acgtggaaat accaccgtta agtaaatata ttacgcgtcc tttaattaaa
     4201 aataatggat cggcaacact aggaaacttt gctttgggct tgaatcaatt acaagaataa
     4261 aacatagaaa aaatatcgat tatcaggtat agtagttcta tagagttctt tttttcgata
     4321 gttaattatt gcattattta ggagagacaa gagaatgcgc ccaaaattag aagatgttgc
     4381 caaaagagca aatgtatcaa aaacaactgt ttcacgtgta ttaaacaatc gtggctattt
     4441 aagtcaaaaa acgattgata atgtttataa ggctatagaa gaactcaatt atcaaccgaa
     4501 cgttgtagcg cgtcaattat ttcaaaagaa aactaacatt gtcgggttac tatttcctac
     4561 agtagccaat ccttttttta gtgaactcgt tgaggcattg gaaaaaaagt tatatgaaat
     4621 tggttataag gtattaatag gcaattcgat gaataataaa gaaaaggaaa caaattattt
     4681 aaatcagtta ttgtctgatc aagtagatgg tttaattgtt ggtacacata accaagggat
     4741 tcaggaatat aaatatcaga atttaccgat tgttgcaatt gatcgagtga tgaatgaaga
     4801 tattccagtt gttgaatccg ataactacaa tggtggaaaa cttgcaacca agttattaat
     4861 tgcacaaggt gcaaaaaata ttattcatac aaacggtcca attgacttac agactccggc
     4921 taatcgtcgg agactagcct atgaagatac tatgaaagca tatcagttaa ttccacgaac
     4981 tgtgacatta gatttcaata tttcttatgt aaagaaaaaa cagatttttt tccaaatgtt
     5041 tgaggattat cctaaaatag atggtatttt tgcttcaaat gacatcgatg cggccctaat
     5101 attacaagta gcaaaagaaa aggggttaaa tgtaccagct gatttattag tagtcggata
     5161 tgatggtact ttaatgactc ggagtatatt accagatctg acaacagtaa tacaaccaat
     5221 taatgatata gcagacacag ctgttgctat tttgatgaaa cgaataaata aagaagaaac
     5281 aaaaaaggaa tatattttac ctgtcacatt atggatgggg aagacatcag taaagaaaaa
     5341 ggacaattaa atttataaag actatcaatg ataatttcat tattaattcg tgatataaaa
     5401 agttgttaaa tctcttataa tagactatga tacaaaatca tgcttttaat ctgtgtagat
     5461 gttgccaaat atttgaaaga catagaactt gtcagaaaaa ccttaacaat tcctcaatgg
     5521 gcggataagc taggcaaaag ggctggcata aacttttcag tccttctaac agaatcaatt
     5581 gcagataaag cagataacat tcttctttct ggaagaaata attagaactc aaaagggtca
     5641 gaaacttaaa agtttttgat cctttatttt caagtctgat ttgacagatt ttcggcagct
     5701 catttattta tggtcagaaa tagttgctga aattttaatc tattagcaaa tagtccagaa
     5761 cgtatgaaga ggattcggat atcatttgtc attaacacca taaatattta taaaattcgt
     5821 gttcacctac aattaaattt aatatataat attcgttgta agggcttata attttttgtt
     5881 aatgaacaag atatgtaatt gatagtgtca atagctattc aaaaatgtag gcttttggcc
     5941 cataaataac aaggttactt atatgataac tttttttctt tataatcttt aatccgatag
     6001 agctttccag tagtttgaat aatcttagcg tggtgcacta atcgattgat tagtgcattt
     6061 gtgttcagtc cattgagaca taatagtgga tagttttact gagaaaattg ataaaaaata
     6121 gagatataat tcaaatacgt atataatgaa actaaagata aatacggagg aaaatatatg
     6181 attataaaaa acttaggaaa tgttaaatca atagatatac acgttaaaga tttgctgatt
     6241 tttac
//
LOCUS       DACDPS010000106         6054 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.106, whole genome shotgun sequence.
ACCESSION   DACDPS010000106 DACDPS010000000
VERSION     DACDPS010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6054
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.106"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(195..482)
                     /locus_tag="IWJ07_002443"
     CDS             complement(195..482)
                     /locus_tag="IWJ07_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP9333977.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(502..726)
                     /locus_tag="IWJ07_002444"
     CDS             complement(502..726)
                     /locus_tag="IWJ07_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HAP9333978.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(1181..1396)
                     /locus_tag="IWJ07_002445"
     CDS             complement(1181..1396)
                     /locus_tag="IWJ07_002445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333979.1"
                     /translation="MEKMVAAMEMKKEILMTKKHLLFGSILLGILSFSPMLLSVANKG
                     NKKGELKLNPNYYNSQNEEVFVEKLDK"
     gene            complement(1366..1899)
                     /locus_tag="IWJ07_002446"
     CDS             complement(1366..1899)
                     /locus_tag="IWJ07_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP9333980.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(1920..2864)
                     /locus_tag="IWJ07_002447"
     CDS             complement(1920..2864)
                     /locus_tag="IWJ07_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP9333981.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
     gene            3501..3587
                     /locus_tag="IWJ07_002448"
     CDS             3501..3587
                     /locus_tag="IWJ07_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP9333982.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(3708..4397)
                     /locus_tag="IWJ07_002449"
     CDS             complement(3708..4397)
                     /locus_tag="IWJ07_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333983.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(4404..4682)
                     /locus_tag="IWJ07_002450"
     CDS             complement(4404..4682)
                     /locus_tag="IWJ07_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333984.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(4642..5556)
                     /locus_tag="IWJ07_002451"
     CDS             complement(4642..5556)
                     /locus_tag="IWJ07_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP9333985.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(5538..5918)
                     /locus_tag="IWJ07_002452"
     CDS             complement(5538..5918)
                     /locus_tag="IWJ07_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP9333986.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
BASE COUNT         1602 a         1112 c         1022 g         2318 t
ORIGIN      
        1 cgcttgtttc accttcggtg aaattgttgc aaatcaataa ttaggtagag ctacattttt
       61 ttcgttgttc aaagattctt atgccattta ctattaataa atctataaat atgataattt
      121 ttcggctcta taatttatgg gactgatgaa tcagaattga ttcttcagtc ccattttcat
      181 tttattccaa agaattaact ccaaatacca atagaagccc atcctctata tacttgagat
      241 tccttactaa caatttcttg taattctatt aaagatttag gtaatccaaa tttatgtttt
      301 aaaatatatc ttccaagaaa actacttaat ttcatactaa cttcaccaac actttttccc
      361 tcactgattt ctttagaata aagaaagagc ttttctttta agtcaggata gtcatctata
      421 gttgcttcat tgtatacttt actaattgca tttaaaagtt tttcttcttt agtaaaatcc
      481 attataataa cctccctacc actaccttct aggtgcccat ggaccatgat taacccaacc
      541 attaacaata atttttccaa tttctccttt agcttgattc caatctaccc aacatttttc
      601 tttgttacaa taaagaccat ttccatagta agtagctgca tcaactttta ttcctgtacc
      661 gatgccagct agacaagttc ctaatattcc tagaataaca caatgtttta atactttctt
      721 tttcatgatt gtttttcctt ttcgtttttt taaaattgct tgttatatta aataatctta
      781 gtaattttat tatataacat tttattatat aacaatgatt aacgtcataa aaagtagttt
      841 tgaattcata atcgtttaaa taaaattata agatcagtta tttaaacagc tagtagacat
      901 aatgtcgata ctaccaagta acgcctgtac caagggatcc aggcgctgat tttgtgaatt
      961 tttattggtc taggttaaaa gctcttttta aggcttggtt ttctgcggtt gtttctttct
     1021 aattctttgt gaatattgat cggtcatttt ttaaatactt gttatttttg tttgtgtttg
     1081 ttatgctttt tacatatctc atattcattt attattcttt tctaaggtta ataataggat
     1141 aatctctttc agaaagactg ctcgtacgag cagtcttttc ttatttatca agtttttcta
     1201 caaatacttc ctcattttga ctgttgtagt aattaggatt gagttttagt tccccttttt
     1261 tatttccttt gttggccact gataataaca ttgggctaaa gcttaatatt cctagtagga
     1321 tagaaccgaa tagcaagtgt ttttttgtca taagtatttc cttcttcatt tccatagctg
     1381 ccaccatttt ttccaattct ttgtttgttc gtccattgtt tctgttgctt cagaaagagt
     1441 cgttgaacta ttctctgaag actcttccgt ataagtcaac tgcagttgct cctgaagttt
     1501 ttcgatttgt tggttggctt gcagggtgag ttgttgttgc tggtcgagta gtttttgtat
     1561 atgagaatta ttttgctctt tgagggcaag ttgttctcgt aaaaattcat tttcttcacg
     1621 caactgatct agcaacttgt ttgcaaccac gtttttgttt ggtatataag catttttatc
     1681 gttgttttgt tcatgtttat gcaacttatc tagcaacctt tgattgtcta agttgcgagc
     1741 aagttgcaat actccagaac gcaatacaac cttataacca cgctcatttt tagctatcat
     1801 ggaagaatct aatttttgga tatgtttcga tattgcttgc ctactcatct taaaaagatc
     1861 tgacaattcc tttattgtaa aggtttcttg tggttgcata ctggttgcct cctaggttgc
     1921 tactggaata aattatctaa atcagataat aaactttttt tctctttttc cgaaagtgcg
     1981 gactctgttt gtttttcaag agcttttttc tctttttcta gttgtttgat ttgttggagg
     2041 agttcaagtt ctcgtttctt ctgtttgatt ttttcgttaa ttgttgtagc gggttcttcg
     2101 tattcttttt gtttatcagc taaagccaat gctttatttt ggtcttcttt ggttggtgca
     2161 tgtttgttaa agtcgtctgc ttttttgatc tcaggtttga acgtaaattg atagccaata
     2221 acaggttttc cccgtccttt tccgtatttc tttttgattg ttagtccttt gaagattgca
     2281 gttagttctt gacgtatagg ttttataact cgtttatcta tatcaggttg cttataactt
     2341 ttaggaatat ctaataattc aagaaagtct tctttggaga aatatgcgaa acctttggtt
     2401 ctgtattgct ttaacagacg aaacatagtc tttgcatagc cactttgtag ctcgttgaat
     2461 tgttgtaaag aatagcgtac ccactcgtct aaattattta acaaaggcag tgctttttcg
     2521 taaattcgta tatctacgta aggattttct acgtccccgt tgattttaaa ttctgtaaac
     2581 attacaaaca tatcgtaaga aagcccagac tcacttctac gaccaaagcg taaagataag
     2641 attttttcat aagttgcttt taaatcttct acaaatcgta catttgcagt tgctttgtag
     2701 ttacttaaat cttttagttg gtcaaacgta aaacgaactg tcttatcccc tttttcacgc
     2761 attctagaca cgatggaaaa aaacaaattc atttcaaccg gtgtaaattt tcttaaagga
     2821 attgtattta attcatggtg gtattttaca atctcattag ccatataatc acctcaatcc
     2881 aattatgaat gacatttttt aaatgtcaag acattcatat ttgacgttca aacagacaaa
     2941 atgtccttca taaacagaca aaatgtcatt cataaacaga caaaatgtca ttcataaaca
     3001 gacaaaatgt ccttcataac attctgtatc cattggtaga gtaaggacac agcttacggt
     3061 aaagaggttt aaaaagaggt ttaaaaagag atataaaaag aggggggcta attttcccgc
     3121 ctaaaaacca cttctttttt ctttaaacag gttcgcgcat ttttcaaaac tgccaatcca
     3181 aaaccttgct tcctacggtc gccaaaaagg tgtgatcgct acgctctcac attgtactgc
     3241 gcagtcgcct aaaggctcct ttgctaaaaa catgttggta aagaggcctt tgagcggttc
     3301 tgaatgctta tctaagccgt tttaagacag tcttggataa aaatccttat ctagctttaa
     3361 agcgccttac aggcggtcag ggcaagccct aacaacccta aaataagcga tacttaaaaa
     3421 caccacacaa gtttaatttt ttaagatata ataacttgtc gcctatccga cagtcttgcg
     3481 actggaagga ggtgtatcta atgtttgaat tgttcatttg cccccttctt atagaagttt
     3541 gtgtctcttt attcagttat tggttgaata accgaaacaa gaagtaaaat caataaaggc
     3601 gacatcaacc tgaaaaaaag ccccagagaa tccgacctct ctggggcttt cgtgtatcta
     3661 atgtttgatc atttgcatgt ttagtttaac atgattgccg atcaaattca attttgctgt
     3721 tgctgatcta accattcctt gacacttttt agataattta atgccgtttc ttttttcgtt
     3781 ggattttgta tgttcgttgc ttgctgaagc agtgctttat aacgttcgta ggtaactgat
     3841 tgttgccgtt cttttcgttc ctgttcacta gttgaaaaag ggattgtatc actgaataaa
     3901 ttataacaag cttcttctgt ctgcgtatct ttaaaatatt gcacgtcttt tttggttcgc
     3961 tcatagttgt cttgaacctc tttttgctgt ttttctggca agttttgata aagacgagtg
     4021 agtttttcca tgtttttatc cgtcaaacgt ttttcttgat tcacaaaaat atcgttcatt
     4081 tcctcataac aagtggtaaa ctcttccatt ttttgtgtta acacttgctc ctttttcttt
     4141 ttatcacatg ctgcttcata cttttgagtg atatccccta ataaaatact tcttgaaata
     4201 cgtttatttc cctctaaaac gccgaataaa ccacttaaaa cgattgataa tgtaaatata
     4261 acccctggca ataaaaacct cttaaatcga attttagagc cttttaatga atatcttgtt
     4321 tgataagcct tatagctaag ccaaataatt cctaaaatac tggcaaaaat gagtagatag
     4381 ggtaaataga atgccatttc tatttattct ccccatacca gccaaaaggc taacagtgtc
     4441 atgattaagc aacataaggg ggcaagaata tccattgatc gctgcataat tacttttttc
     4501 tgttctttat ttcgctggtt taatagcgca tggtggaatt ttttgacttg tgaatcaatt
     4561 gtagtttgta ttctttcttt ggtataaggt gcaagaaaag cacgttgttt ctttatttca
     4621 ttttctatgt cttgtaacgt tttagaacga taattcttgt tggtagtctg ctcctctttc
     4681 aacgtgttgg tcatggtttc ttcgagtcgg tctaatttcc ggttgttcag tcgtaagagt
     4741 tcgttgttct cttcgtactt cttttgcagt ttcgcgaatg ctagctcgag ctcttcgtag
     4801 ctcgttggtt cgttgttgat tgtaggctcg ttctcgtctg tccagctcat gtaaaatcgt
     4861 ctccttttcg taatcctttc ctagtttgtt ggatcgtact tttttctttt tgtggatcaa
     4921 ttcgtaagta aaatttttcc cacgattcaa gcaatgaatg ccgaatgttt taagagaatc
     4981 acaaaattgg ttatacgttc gagttttttt gtttttcatt gtttgatcaa tcgcaaaccg
     5041 aatttcgtct ttccaaacag gttcattttt ttcttttaat ttgatttcag acatggttcg
     5101 tttttcgggt ttcttccctc gttcagtgat tgttaagtca tgttcaagac aaatctcgtc
     5161 atttaggtca taacatttgt ttaaaaatgc ttgaccatgg gcttgaaatt tgttcccagt
     5221 ttccagattg acggagttaa tgacaatgtg gttatgaata tggtctttgt ctgtatgtgt
     5281 gtaaatcgcg atttggtggt caggggctat ttttttcgca tactctgccc ctagttgatt
     5341 ggctttttca gcggtaattt ctcctggtcg aaaagactgg atcaataagt gtgcttgtac
     5401 acgatcgtct tttccataca gcatgcgaat ttgtttcatt tcgcttttgg cataatcaat
     5461 gtcacagttt atccctgttt tgacggttgc tcgtggttcg gcgtaattga tcgctcggct
     5521 acaagaagtg gttcggctta ctttaactgt tgccatatct tttgtagctc cttttgcatg
     5581 atttttaggt tttcggataa gcgttgggcg tcgccaggat caacgtcgtg ttggtgtgtg
     5641 ttgcaccact tggcaatttg attgatattc ccgccagctt tggctaaatc gcgcgcgatt
     5701 gcctgagcaa tgtctggcgc cactttcggt gtcaccagac gcgcgttttg tgccttggct
     5761 ttcacgaaag ccggcacact catttgcaaa gcttcagctg actgcttcag ctttgcaaat
     5821 tcggattcgc tcactcgaaa actgatttgt tttggctcgg atcgataggt atatttttta
     5881 gaatgattgc tagccaaata ttgattttgt tcgctcataa gtcccaccct ttcagtacgt
     5941 cctacggacg tatcgttcgc aataccattg tacctaaaaa ggtacaataa gtaaagctca
     6001 ctaaagctta ctaaagggct tgacaagctc tatatttgct agccaaatac ctac
//
LOCUS       DACDPS010000107         5943 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.107, whole genome shotgun sequence.
ACCESSION   DACDPS010000107 DACDPS010000000
VERSION     DACDPS010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.107"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(163..465)
                     /locus_tag="IWJ07_002453"
     CDS             complement(163..465)
                     /locus_tag="IWJ07_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333987.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(685..1051)
                     /gene="ssrA"
                     /locus_tag="IWJ07_002454"
     tmRNA           complement(685..1051)
                     /gene="ssrA"
                     /locus_tag="IWJ07_002454"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1222..1995)
                     /locus_tag="IWJ07_002455"
     CDS             complement(1222..1995)
                     /locus_tag="IWJ07_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9333988.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2139..2489
                     /locus_tag="IWJ07_002456"
     CDS             2139..2489
                     /locus_tag="IWJ07_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP9333989.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2470..3186
                     /locus_tag="IWJ07_002457"
     CDS             2470..3186
                     /locus_tag="IWJ07_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9333990.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3186..4634
                     /locus_tag="IWJ07_002458"
     CDS             3186..4634
                     /locus_tag="IWJ07_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9333991.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4704..5213)
                     /locus_tag="IWJ07_002459"
     CDS             complement(4704..5213)
                     /locus_tag="IWJ07_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333992.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5203..5928)
                     /locus_tag="IWJ07_002460"
     CDS             complement(5203..5928)
                     /locus_tag="IWJ07_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333993.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         2012 a         1039 c         1148 g         1744 t
ORIGIN      
        1 gagtggtagt taattagtga cttatttttg attttatagc actgatacta taaaatatag
       61 atatcatcat attacacaat cttaatacta acttaaaaaa tatccctttt ataagtatgg
      121 tgataaaatc cgttccgggc tacctttttt aggtagccta ctttaatctt tatacctttc
      181 tggatcaacg aaagtatact tcaaatagtc ataacgccga tgatcgctcc gtgcgtctgg
      241 cacatcagtc acgatatcaa acaaaaaata cacatctttc ttcattctgg tttttgttgc
      301 cggaatttta aagtagttct tattagagta atagagattg attaataagc tatcttcgat
      361 tgctaaaaag aaaacttctg aatcccacac cttataaaaa tctttgacaa atctattcga
      421 aggatcaaat ttaaaccata attgtgtttt tccttccatc aacataatgt tcacctcaaa
      481 aagagtatac gaactaatgt tctttttgta aagtaggaat atcattgata atatggatgg
      541 aacatatata ttaaaaagtg ttctttttga atgctatttt gaatgccatt ttcattcatt
      601 taacgaaaat ctgcgaaaat aaaaaatgct caaaccctta taaaataagg atttaagcat
      661 ctagcgaaat ctgctgaaat ctcacaatgg agacggcggg agtcgaaccc gcgtccaaac
      721 acatcgccac ttaaaaatct acgttcatag tcaactcatt taaggattca ctttacagct
      781 tgccgagtga caggcattct gtatcgctag tctgatgttc tctttttcaa cttacagacg
      841 gaaagttgaa acgtatccca ctaaagttag gacccttact cgagcacatg ggcgatgccg
      901 agaggatcta cgctaactgt ttttaggcag ctaaagcgta agagttgttt tcgtttttag
      961 cagttatatt taactgtaac gtttttacgt agacgtaacc tacgaaacgc aattcaagct
     1021 cgactgtgcc tgtcgaatcc gtaacgtccc cgttagctaa caagggttac gtgtattttt
     1081 catacaaaat tagtatagca tatattggga taaattcaat aaaaaagcga atcatatccc
     1141 attatttata taataactgc ccctttctta agaaaaaata agcgattttc ttaagaaaag
     1201 aggtagcttc aaaaaaagat cttaaatcag atcaaaatac ttcaatgcgt ggacaattcc
     1261 gccttctgta tttttctttg tgataaaggt agaaagttct tttaatccgt cataggcgtt
     1321 gcccatagcg attttgttgt cgcaagcttc caacaaggca aaatcattcg gaccatcgcc
     1381 aaatccaaaa gtaggaactt tttccagctg aagttctttt tgcagacgtt tgacacctgc
     1441 cccttttgag attcctttct tcactgtatc gatcgagtaa ggaccattcc gataaaaagt
     1501 tagctcagga aaatgttcgt gatagtattc gtcacctgat tgagataaga tcagcaacat
     1561 atttactgtg ttttctttaa agcctaaagg atcaatttcc ggaacatctg aatgaatgaa
     1621 gtcgtaagca ttgatcattg tttggctatg accggtacac cagattttct gatcgttata
     1681 gtaagaaagt tcatgaccgt tcaactgtac atgatcatac attttttgac attcttctga
     1741 agaaaatgtt tgagaataga tttcttcgcc gtctactcga atgaaagaac cattcatggt
     1801 gaccgcagaa gtaatgcccg catcttccat gatagagtgg atttctgctt ctgtacgtcc
     1861 tgtagcgatg acggggagaa cattgttttc ttttaatgcg ttcatagctt ttgccacttc
     1921 gggtgtgatt tttgatttcg catctagtaa tgttccatca agatcaaaga aagtgattgc
     1981 acgataattt gacacaaata aaactccttt tttattggaa taagtttatc ttaacaaaga
     2041 atgaacaaaa gacaaacctt tcgtgaagaa ttcctgaaca tattggttca gtcaacaatt
     2101 tagagtatac tataagaaga gaatttagga gggaaagaat gatttttttc agtattggta
     2161 tcttattctt aataattggc agtctttttc ggattctgcc ctcaaaagga aatctgcctt
     2221 tttatgggta tcattcgcct cttgccgcaa aaacagacgc tcattggcgg ctggctcaaa
     2281 agacaagcgg aaactggttt tttcttatgg gcttactgat ggcattgatc ggctattatt
     2341 taaagacatc tgggcatacg aattattttc tgattgaaat gcttctgcta gtatttccaa
     2401 ttatgcctat ttttattatg acagaaaaga aattacagaa atatgacctg gaaactggag
     2461 ggaacgataa tgaatatttt aatgattgaa gataatcaat ctgtttctga aatgatgcag
     2521 atgtttttct taaatgaagg ttgggaagca actttcaagt atgatggaaa agaagggctc
     2581 gatgcctttt tagaaaaccc acaacattgg gatatgatta cactagacct gaatttgccc
     2641 acactggacg gtatgctagt aggaagagag atccgcaaag tttcaagtac tgtgccgatt
     2701 atcatgctga cagctagaga ttctgaaagc gatcaggtga tcggcttaga gatgggggca
     2761 gatgattatg tcacaaaacc tttcagtcct ctgacgctta ttgctcgaat gaaagctttg
     2821 catcgccgta gtgagttggc agaagcaaga gaagaagcag tagtttcaga tgatcatttt
     2881 gatatcgaga ctagtcattt caagatgaat acgaaaacac gagaagcgta tctggacaac
     2941 cggccaattg aaggactgac gcctaaagag ttcgatctat tgtttacttt agcaaaaaaa
     3001 ccacgtcaag tattctctcg ggagcagttg cttgaattag tctgggatta tcagtacttt
     3061 ggtgatgaac ggacagtgga cgcccatatc aagaaattac gacaaaaaat cgaaaaggtc
     3121 ggaccacaag tcatccaaac tgtttgggga gtcggctaca aattcgatga ttctggtgtt
     3181 gcctaatgcg ttatttgtat cagcaactct tagctttctg cggaatggtc gcgttgatct
     3241 tattgatcgt tggagtttct tttacacaac tgacaaaaca aacgattgaa gaaaacaatt
     3301 accagcaact gtttggatat gctgaatcag ttgaaaagac aacgcaaacc tatgccgata
     3361 cgctcccgca gtttagtcaa gatcaggcgt ttcaaaatgc attgctgctg acagagcaag
     3421 tactgacaga acaaaatgtg aactttatat ttatcgataa aaacgaacgt gtcatttatc
     3481 cgactaatac agctttactg aattttacct taacagctga tcaatggaaa aatctgagga
     3541 atggacgtca agaaaaattc acatcgaata aaaatatttt aggacaagca caggcgacct
     3601 cttacgcact tgtgccattt aatttgaatc atgaattcta tggagcactt gtagttagtc
     3661 aacctgcgcg aaatatcgat aacagcgttc gtccaattac actgaacctg ttcaaaggat
     3721 ttattttttc cagtatcgtt gcagttattg ccagctatgc ttttgcagca ttccaagtta
     3781 agcgtatcaa tcggttaagg aatgcgacga aagaagtgac gaatggtaat tttgatgtac
     3841 agctccccgt acatgacaaa gatgaatttg atgagttagc agatgatttc aataaaatga
     3901 cgaattcatt gaaagaatct caagcagaaa ttgaagaaca agaaaatcgt cgaagacaat
     3961 ttatggcaga tgcttctcac gagatgagaa cacctcttac gacaatcaac ggacttctgg
     4021 aagggttaga atacaatgca atccctgaaa atcagcgaga aaacgcgatt aaattgatga
     4081 aaaatgaaac agagcgtctg atccgtttgg tgaatgaaaa cttggattat gaaaaaatcc
     4141 gtacgaatca aatcagtatg gtcattaaaa aattcaatgg aacagaaaca ttgaaaaata
     4201 ttgttgcaca gttagaagct aaagcagaag cagcaggaga tacattgaca ttgaaagccg
     4261 ttgatgatat tgatgtttac gctgattatg accgatttgt ccaaatcatg gtcaatatta
     4321 ttcaaaacgc gatacagttt actgaaaacg gccaaatcac gattacttta gaaaaaggct
     4381 atcttgaaac gatcatcacg attgaagata cgggaattgg aatgtctgaa caacaaatga
     4441 aaagtatctg ggatcgttat tataaagtag atccttcaag aaaaaataca aaatatggtg
     4501 aatcaggatt aggtttacca atcgtgcagc aacttgtacg cttgcataaa ggaaaattag
     4561 aagtagaaag cgaactagga aaaggaacaa aattcgctgt tcgtttgcct gacgtagaaa
     4621 tcaaaaaaga gtaaaaaaca agcggtataa aagatcatga atcgatcttc tataccgctt
     4681 gtttttacta taaaatgatt gatttagccc gcgggctcta aacgatcaat ataaggactt
     4741 tgagcaatag tagcatcatg agtaaagagt aagatacctt gattttcttc atgagctaaa
     4801 taagaaagca agtggagtag ctcttggatc tccaatacac taagttcatc aaatgtatct
     4861 tcaataacaa tattgtttcg cttggaaagc aaggcatgaa gtaattgcag ctttactttt
     4921 tcaaaagacg tcaactcagc tgccgcacaa ttaagcaagg aaggagagat atcaagtttt
     4981 tcttgatagg ccaaacgatc ttgtttgatt tctttttccg gtacatccaa caggacatgg
     5041 tctttaagat tcagataagg aaacatcggc caattttttt ggatgatcgc aaaattatcc
     5101 agttttgcaa caagaacctc ttttttcaag tgctccaata ttttatgctg tttttcgaga
     5161 ggacacaaaa taacggtcaa gtgttcagca tgaatcaaat attcaggttt cattacgtcc
     5221 aggcctccta ctttgcaaat gatggataaa aaaagtgaaa gggaatagac taagaatgag
     5281 acagcatcca ctgagcccaa taagagtttg ggcaaatccc ttaagtatat ctgtgaaaaa
     5341 aagatcattt tggaaatcga tggtaaaaaa agtctgttga tccatcggaa ttgtaagtcc
     5401 gcttaccgaa gtcaagatat cacggatatc tggcagttca gtagtcttta agacaagaaa
     5461 attgatcctt tgaagaagcg cttcataaac tgactggaag cttacgatcc acaataaaac
     5521 caagatacaa caaagaaaga gaggaaagaa cagctgccag aaaataaaaa ggaataattt
     5581 ggttttagaa agaccagtca ggtgccaatt gatcaattcc tgcttgtttt gcctgactga
     5641 atagtaaaag aaaaagataa aaaggaaggc aaatagaaaa aataaactga atatgccttt
     5701 tatataaaat cggctagcta attctaaagc cggaagaagc tctgtagatg cagaagcgcc
     5761 aataaatgaa agccgatctt ggacttgatt ggctaaagcc gattgcaaat ggtaaagagt
     5821 caggcatgct atcaataaaa aagcagaaaa gcttgaaaat aaacaataga gtattgtctg
     5881 ctttttgtaa taaattaatc gagcagtagc atagcggatt attttcatac ttttcacctc
     5941 tat
//
LOCUS       DACDPS010000108         5938 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.108, whole genome shotgun sequence.
ACCESSION   DACDPS010000108 DACDPS010000000
VERSION     DACDPS010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5938
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.108"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            361..642
                     /locus_tag="IWJ07_002461"
     CDS             361..642
                     /locus_tag="IWJ07_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333994.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            644..1060
                     /locus_tag="IWJ07_002462"
     CDS             644..1060
                     /locus_tag="IWJ07_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333995.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            1682..2836
                     /locus_tag="IWJ07_002463"
     CDS             1682..2836
                     /locus_tag="IWJ07_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HAP9333996.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            2830..3252
                     /locus_tag="IWJ07_002464"
     CDS             2830..3252
                     /locus_tag="IWJ07_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9333997.1"
                     /translation="MVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            4422..5390
                     /locus_tag="IWJ07_002465"
     CDS             4422..5390
                     /locus_tag="IWJ07_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9333998.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            5407..5931
                     /locus_tag="IWJ07_002466"
     CDS             5407..5931
                     /locus_tag="IWJ07_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP9333999.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKAEKKWWQFWQ"
BASE COUNT         2252 a         1015 c          963 g         1708 t
ORIGIN      
        1 ttaataaaaa ttgctggttt tattcttctt tttgcttttg cgagcagagc aacatggacc
       61 catgttgcga tattttggtg gcgccccaat ccccgttttt tcgttgaatc ttactcggac
      121 tgcactcgtt ttacagctga tttttctgaa ttttgaaaga gcataaggaa acgtggtatc
      181 actttcttac gaaaggtgct agataggctg tgcctatcac gtttctcaga aaaaaatttg
      241 ctatcacttt tttgccattg tggctaggct atgtatagtg ttttggcgaa aaatagctat
      301 acaaatacta tatttgtatg gtatacataa attatacgct gatacgaaag gaaaatttcc
      361 atggacattt tagttcgaaa tatcaatccc aaaattattg caagaataga taaacgttgt
      421 caggaactca caaccaaaac gggcaaaaaa tggagtcgaa ataaatatct aaaagttcta
      481 atcgaaagtg attttgaccg tcaacttatg aattacaaaa aagatcaatt cgatttatta
      541 ctcgaaaaat ttattgccat tcaaacagaa aatacgaaca tactgaatga ctatgttcac
      601 acaaacaacc aactcattga aacacttatc ggaggaatat aacatgagaa aaacttctgt
      661 tcaaaaacac gtaacccttt atcccagtca cgttgacgtt ttagattcca tcatcgaaaa
      721 aaatccagac gtcaacaatt attcagaagc cattcgtttt ttatgtatga gttatgaacc
      781 tgataccaat acaaccacaa ccagtaacca agcaaaactc aatgccatgg gaaaagaaat
      841 ttctatcatg acagaaatca tggcagaatt tgctgattta tatctaaaag atactggtgt
      901 tcttatcggt acaaattcta aagtgtatca agaagtgaaa gaaaaaattg aaaatcaact
      961 caatccttcc cgacgaaatc aacccaaaaa tcgtcaaaaa gaaaccaatc aaacctttac
     1021 ttctggaaat tttgacccaa caaactttct aaaaagataa aaaaagaaac cgatccctaa
     1081 aaaaattcct cgatttcttt ttagggatca gtcttccagc aagatttgca agttggtacc
     1141 aacttgcgaa aaaaaagaaa aatccgctta tgcaacttct tctttttgta ttttgaggcg
     1201 ctaccccaat ccccaagtat cggttattta atagcgcgga aatccaaaga ataggacgta
     1261 ccctgaaaca aaaaaagact gctctcacga gcagcccaaa atgagattat cctattcatc
     1321 aacataaaaa tattacaaag tgaagaacta gagaaagaca aaaaaagcat atcaaatcct
     1381 ctcaaaaata acaagtcttt aaaaaatgac agataaaaat gcacaaagaa ctaaaaaaga
     1441 gaaatcgcgt aaaatcaagc attagaagct agtaactagc tagaccaatt aaaaatcaca
     1501 aagtgagcgt ctctatccat tggtagaggg gctacttggc atagtgtaca ttatgttaac
     1561 taacgctata aataacgatt atgttcccaa aacaatattt tatgaactat aacaaaaatt
     1621 tgttatcgca tattgtatat tttaattata ggtgtatacc acttaaattt aacacaaagg
     1681 cgtgattatt ttgaaaccca aaaagaaaat ttatctaggt ctcctctttt ccttgatgat
     1741 tggaattttt agtataggaa ttacaaatac ctatgcttca gaaaattcta atttcaatat
     1801 caactcccaa ccaattgaag aacaagcaat taattttgcg aaaactgatt ttgaacactc
     1861 cataacttca attcttttaa tggaaaataa caccgaaact gatattaact atactcttgg
     1921 ttcgccattt aaaatgctcg atggagaaaa tacggcaaat agttctattt tctttccagt
     1981 attttcagac gctgataata cgattaaata tatttatacc gtcaaaaaaa atacaaatgg
     2041 agaatattca ggaagtattt cacaattttt agcaaaagaa cttcaaaatc tagtagatag
     2101 taatataaca gatacaacct tattttttca agataatagc atttattatt ccgataatgg
     2161 acaaattaaa ctactttgga aatcaccttt aagtacagaa aaaaatggtg tttcttcctt
     2221 tacagaaaaa aatgatctta tatccgtaaa tataaaaaat aaaattaaaa atttctctaa
     2281 aaaacaaaat tttcctgcgg ttaaacgtgt aaaaagtgtc tcctccggag ataactttat
     2341 tatgattaat tggcgtatag cagaaaccca aacaagtttt ccgtggtgtg ctgcttacgt
     2401 agaagctgct atcttatgta acaaaacaga tgtacgtgct acttcagcta gagccattat
     2461 gcattatacc tatcctaatt taaacaacag tcaaattgaa tcaaaaagtc ttaatcaaga
     2521 tcaaataata aaatatgcaa attactgtgg tgtttatcct aaaaaagtag cacgaatact
     2581 aaacctttca gaggtccaaa cacaactaag aaaaggaaat gccatttata tgggaactgc
     2641 tggttatgga aattatagta acagccgtca tgcacttgca ttatttgggt gggtcaatca
     2701 aggtggttat caaacttact atgtgtggaa tccttggtat aactacccaa ctgtaataag
     2761 tgcaaatgca ggaccagtta caattcctgt acctggaggt ggttttactt gggacaatac
     2821 cattacaaat tggtaaataa acccattgct ttagttatgg gtctagtgtt agttcttttc
     2881 ttaactagtt gttcaaaaaa acaagaaata caaaagactg atctgattcc ttttctagga
     2941 acttggcaaa ctaaagatca ttcagaacgt ctagtaatca ctaatttaaa tacttcaaaa
     3001 aatatagaac aatataccat cttagtaaaa gagaacaatc ataaaattca aatcattgat
     3061 aaaaatttaa atataaatga acaagttttc atttacaaag caacaaataa tttactaaca
     3121 ctgcgctcta ataaaaaaca attagattac acttttagtt tggattctcc aaaaaagctc
     3181 gtatttattt tgacaacgaa tgatgggaca gaagggacag ctaaaccaat tgaatttttt
     3241 aaagctgatt gatgaatagt tgtttgatat tacaaaacaa taatttcacc gaaggtgaaa
     3301 caagcgaaac tttacaaaaa gtggctagca tttgctagcc ttttttgtat tgtggagcgt
     3361 gggttttgag ggggattcaa gggggagctt gcgccccttt gaaaagcggt tatttgcacc
     3421 aaatacgtag gtatttggct agcaaatata gagcttgcca aacactttag tgagttttgt
     3481 agaaagtacc tttaaaggta ctttcacatt acgaacgata cgtccgtagg acgtactgaa
     3541 agggtgggat ttatgagcga actaatgcaa aatttggcta gcaaaaaaag ttaaaaaata
     3601 tatgctaata atcgagcgaa atgaacactt tttcaagtga gcgaatccgc catgacaaag
     3661 ctgaagcagt ttatttcagc tttgtcatgg cgtgtgccta gacttttaaa aaagtcggca
     3721 cacgagcaaa tatctttatc aagctctaat ttgcgtttta agacgtttga aacgtctaag
     3781 tatataaata tataagagta ggtttaagag tgcttaaatc gaattttaga gggcattcag
     3841 acaactttag ggttgttagg gcttgccctg accgcctgta aggcgcttta agaacaagat
     3901 aagggattta tactaagact gtcttaaaac gtctcaaacg aaggctcata gcttctcaaa
     3961 gagttcctta tgaacatttt tagcaaagga gccggaagcg actgcgcagt ataatgtgag
     4021 agcagagcga tcacaccttt ttaagcgact gtagggagta agattcccag ttgagaaaaa
     4081 tgcgcgaatc tgtttggaga aaaaagaagt ggttttttag gcgggaaatt ttgtccccct
     4141 cttttataat ctctttttaa acctcttttt aaacctcttt accgtcggct atatccttac
     4201 tctcccaagg gaatgacgct tttatctaca tcgttttgtc tgtttatcta catcgttttg
     4261 tctgtttatc tacatcgttt tgtctgttta tctacatcgt tttgtctgtt tgtacattga
     4321 attagtacat cgacgatgtt aatataagat gtagataaac aggcattttg tatttcataa
     4381 cgtgtatctc ttgtgcgagt aagaaaggag ctattttaaa tatgtctaat gaaatcgtaa
     4441 agtatcatca tgaattaaat acaattccgt tgcgaaaatt tacaccagtc gaaatgaatt
     4501 tgttcttttc tatcgtatca agaatgcgtg atgtaggtga taaaaaagta caatttacat
     4561 tcgagcaatt aaaagattta agcaattata aagcaactgc aaatgttcga ttcatagatg
     4621 atttagaaac aacgtatgac aaattaatgg atttacgttt tggtagaaga agtgctgacg
     4681 gtttgcaacg tgaacgtttt gttttattta atcaatttaa aatcgacgga aaatctgata
     4741 ttccttttgc tgaaatacaa gttcatgaaa aagccttacc cttacttaat aatcttgaag
     4801 aatgggtgcg ctattctctc caacaattca atgaattaga aagcagttat tcaaaaacaa
     4861 tgtttcgatt actaaaacaa tacagaacca aaggtttcgc ttatttttca aaagaagact
     4921 ttcatgaatt acttgatatt cctaaaagtt acaaccaagg tacgattgat caacgtgtgt
     4981 tcaatccaat acgacaagaa ctaaccgcaa tctttaaagg tttaacaatc aaaaagaaat
     5041 acggaaaagg acggggaaag cctgtcattg gctatcaatt tacatttaaa cctgagataa
     5101 aaaaatcaga cgactttaac aaacatgccc caacaaaaga agaacaaaac aaagcattag
     5161 aactagctaa ccaacaagtt caatctgaaa aagcacctac ttcattacaa gaaaaaatca
     5221 aagaacaaaa gcgaaaattg gaacttttac aacagttaga aaagttagaa agagaacaaa
     5281 atacccaaaa taaagaaaat aatgcacaaa aatcagagat aaattccgat attccttcag
     5341 aactaaaaaa agacttatta gacaatcttc aaaacttatt caaagattga ggggttttta
     5401 gattttatga cagaaaaaac aatcaaagaa cttgctgaag aattaggagt tagtaaaaca
     5461 gcaattaaca aaaaagtttc tgacgaaaat cgaaaacttt ggttttcgaa aatcggaaac
     5521 aagtttgtga ttaatgaaac cggacaaaag gctataaaat caatgttttt taacgaaaac
     5581 gaaaactcaa atcaacaact agttggcgaa aaaacgaaaa ccgaaaacca caaaaacgcc
     5641 aaccaagttc ccgaattaga agtcctttct ctcctagaac aacagttaaa tcaaaaagat
     5701 atgcaactac aagaaaaaga cgagcaaata aaacaaatgc aaaaattact cgaccaccaa
     5761 caacaactca ccctgcaagc caaccaacaa atcgagaaac tgcaggaaca actacagttg
     5821 acttatacgg aagaatcttc ggaaaataaa tcaacgactc tttctgaaga aacagaatcg
     5881 atagaaaaac aatcaaaggc ggagaaaaaa tggtggcaat tctggcaata aaaccagc
//
LOCUS       DACDPS010000109         5873 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.109, whole genome shotgun sequence.
ACCESSION   DACDPS010000109 DACDPS010000000
VERSION     DACDPS010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.109"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            77..685
                     /locus_tag="IWJ07_002467"
     CDS             77..685
                     /locus_tag="IWJ07_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9334000.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            853..2490
                     /locus_tag="IWJ07_002468"
     CDS             853..2490
                     /locus_tag="IWJ07_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAP9334001.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLLDEAKESLENLIQKLN"
     gene            complement(2562..3674)
                     /locus_tag="IWJ07_002469"
     CDS             complement(2562..3674)
                     /locus_tag="IWJ07_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAP9334002.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(3749..5122)
                     /locus_tag="IWJ07_002470"
     CDS             complement(3749..5122)
                     /locus_tag="IWJ07_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9334003.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            5343..>5873
                     /locus_tag="IWJ07_002471"
     CDS             5343..>5873
                     /locus_tag="IWJ07_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334004.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKE"
BASE COUNT         1967 a         1171 c         1181 g         1554 t
ORIGIN      
        1 tacctatgaa atatttctaa atgataaagt taatagcgtc gaaagaataa aaaacggaga
       61 aaaaaaggag tttaatatga attcatttaa gaaaattgtt ttaggtacaa cttttgctgc
      121 tggtgctact gcaatgtttg taggaactac aaatgctcac gctgatgaag tttatacagt
      181 aaaatcaggt gattcattat ctaagatctc acaaaaattt gcaggagaca actcaatgat
      241 cgatgcaatt gctgaaaaaa attcgatcgc aaacatcaac cgcatttatg ttggtgaaca
      301 attaacaatc ccaacttcaa atgattcttc agcaacaaca gaaaacaaaa caacagaaaa
      361 tacagcatct acaactgaaa cagcaacgca agaacataca tacgttgcac ctgttgaaac
      421 agttgaagta gctcctgctg ccccagctgc tgcaacagcg ccaacttcaa gctctgcaaa
      481 agaatggatc gcacaaaaag aatcaggcgg ttcatacaca gctactaacg gacgttacat
      541 cggccgttat caattagatg cttcatactt aaacggcgac tattcagctg ccaatcaaga
      601 acgtgtagca gaacaatatg taacatctcg ttatggttca tgggatgctg ctaaaacatt
      661 ctggttagca aacggctggt actaagccga taaaaggaaa atgatgaaac cgagaaactt
      721 gtttattcaa gttctcggtt tttttgtgtg aaaaataact cgtttgcttc ccacagcgca
      781 gcagtacaaa taaaaactcg aataatgtat aataaagaaa acgttttttt gtttagggaa
      841 tgaggaggag ccatgagtac aaaagagagt ctcgaattaa acgaaatcac aagtggaaat
      901 ccactgttat ccagtattcg atcgattgta gaaacgacat tttacggtaa taatgttcat
      961 gaagtttttg acaggaagac agcttatcaa ctagcaaaag ggagtccagg aacgatcatc
     1021 acagatttaa caatctcaca tgctgaagaa ctagatttac cagctgatgt taggacacta
     1081 gtattcaatg atggaagcat tgtgggaaga acagcaagtg ctcggcggat ctttgaggac
     1141 ttagacaagg aacaaagcaa atacgagaaa attttaagag aagctgtgta tcaaagtcgc
     1201 aagcgccagt tttaccatac aaaagtaatc gttggcctat ctgaagaatt ttctgtgcaa
     1261 agtcatttgc tgattccaga aggatttgag aacaatttgt attcttattt gctgaatttt
     1321 cagccagcga attcgaagat cttagagact tatcaaaagt ctgtttcctt tgatgaggga
     1381 gatctttata tcttttgtga tcccgaatgg acacatgaaa catttccaaa tggactcatt
     1441 ttgtttgatg ctctccataa tacagcggct gtgctaggat tacgttactt tggagaatta
     1501 aaaaaagcga ctttgacgct tgcttgggca ttgggaaatc gcaatggctt tattgcctgc
     1561 cacggtggga tgaaacaata tgctagtaat gaccagacct atacgatggc tgtatttggg
     1621 ttgtccggtt ctggaaaatc aacgattacc ttagcaaaac atacggaagg tcactctgtt
     1681 tcgatcttgc atgacgatgc tttcgttatt tctcgaaaaa atggttttgc tactgccttg
     1741 gaaccttctt actttgataa aacacaagac tatccgtctg atgatccggc catttcttct
     1801 ttccttactt gtcagaatgt gggcgtgaca tgtacaagta aaggagaaaa agtcctagta
     1861 acaggagata tacgaaacgg aaatgggcgg acagtcaaat cacgttatgc gacacccgac
     1921 cgagtcgatc atcttagcga aaagatcgat gctgtttttt ggatcatgaa agatgattcg
     1981 cttccgccaa ttatcaagat caatgaccca gttttagcag cgtcttttgg tgcaacatta
     2041 gcaacaaaac gatcagacgc ggaaaatatc actacaaaaa gacagctaga tgagctagtc
     2101 attgaaccat ttgctaatcc tttccgtgtc tatcctctta gggaagacta tcaagctttt
     2161 cgagagttgt ttttgaaaca gcaaacagcc tgctatgtcc tgaatactgg atatttcgat
     2221 gataaaaaaa tcaaacctga aacgacattg tcaatcgttg aaagaatcgt gcaacaaacg
     2281 gcagaattca agtcgtttgg tccatttgaa gacatccact atttacctat accagatttt
     2341 cctgtttctt ttgaggatac gtcctataaa aatctggtga aacaacgact tgatattcgt
     2401 ttacgattca ttgaagctca gataggaaaa gatctcttgc tagatgaagc aaaagaaagt
     2461 ttggagaatt tgattcagaa attgaactaa aataacagct ttttcaagaa gtaatccatg
     2521 cgcaatatcg ctcgggatcc ttacttgaaa aagctgtttt tctatgatgg atttttcgta
     2581 atataggtgg agatgtcctt ttgataatct tccgtagacc aatgaccagt atttaagaag
     2641 ttggctaata gatagccgat ataaggacct gtggtcaagc cagaagagcc tagaccgcta
     2701 gcagccagta agcttggatc atctggcagt acgccaaaaa atggggaaaa atctgacgta
     2761 taagcacggg tacctacacg gaactcgtat ggccagtttt ttatctgatc tggatcagtc
     2821 aagaactgtt ctgtactctt agtcaatccc ttataagctt cgctagtagg agccaaatcc
     2881 cagccttgat cgttttcatg ggtagcacct aaaaggatct ttccatccat gaaaggaatg
     2941 aaatcagctt ctccgtcaag catagcaaca ggccagtctt gactcatctt ataagatgtc
     3001 tgaaatgcta agagctgccc tttttgcgga cgtatatctg cagcatagcc taattcttgt
     3061 aataaaggct taagtgccgg gccaggtgtc aaaattacac gatcagctgt gtattctcca
     3121 aacggtccct tgatgagcca agaatcgctc ttcttatgca aagtgacttt tgcctggatc
     3181 tgctccattt gtttttgtgc taaacgctga ccaagatgtt ctaaatatgc ttttccatcc
     3241 agacgaccac caccactgat acgcagtgaa actgctggtt tcaataacgg aagtgcttga
     3301 gatgtctgtt caggtgaaag ccattcgatg tttccaattt ctggcgcttc ttcttttcgt
     3361 ttctcggcaa gaacagccaa ttcagctaaa tctttttcag gtcgaagcaa aagtgtccct
     3421 gattgtgcgt aaatcgtttg atcaagttgg aaatctttta taagtttagg gaaaaaagca
     3481 gcgccgtctt tagccagacg ataccattgt ttgtttctgc gtttcgacaa ccaaggggag
     3541 ataatcccag cgctagcttt agttgcctgt cctgtccctt catcaaataa agcaacattg
     3601 aattttgatg tatctaagta actggctagg gtcataccaa tgatcccgcc accgataatg
     3661 gcaatttttt ccataaaaaa gcctcctttt acaaacaacc attttcacac aaaaaaacgg
     3721 cagatgcaac acctgccgta tatggaatct acgctttttt agcaaagaac agataatagg
     3781 cgaatcgagc aaagcttacg agaagcaaca gcaacaagac gcctaatgct gcttttgatc
     3841 ctaatgtagt agacacttca tagttatggg aagtatgatg ctggacgtaa ttgataatga
     3901 ccgctactaa tgaagtagcc acagcgccag aaaactgctg caaggtattg aacattgcgt
     3961 tcccgtctcc ttgtagttct tcattcagtg cattcattcc ggtggtcatc atattgctgt
     4021 aagataatcc gataccgatc atataaaaca catgacaagc aatcaataca cccactgaag
     4081 cgtgtcctat gagtaggaat aatgctagcc aaccaatcaa agaaagaatc agcccagcaa
     4141 aaattggctt cttcggacca tattgatcca gcattttccc ggaaaaagga gaaagcaaag
     4201 caccaaccgc tgcgccggga agcatcgcta atccagcgac aaaaggacta cttccttgaa
     4261 cgatctgaag gaaattcggc aagacaaaag atacaccaag taataaaaac tgataaacta
     4321 agaagccaat caaaaacaag cggaatgttt tatttgaaag aatagacagt cggatcagtg
     4381 gatgctccac ttttttactt cgatagtaaa aaaggaacca gccaataccg ccgatgatta
     4441 aagcaatcca gctggaaact gttcccaacg tactcaaaaa tgttagactt ccactaaaca
     4501 aaagacaaat tcctaaaacg cttatccaat ccaaataacc acttttttgt actgccatct
     4561 cgggaatagc aaaccagccg ataatcaaag aaagaatcaa aacgggcaca agaaacagga
     4621 agatatgatt ccatgtaaaa tgtgaagtca taatgcctcc ataagtgggg ccaattgctg
     4681 gagcaatggc cgttgtcaaa gtaccgacac ccatcatcgc gcctcgtcga ttgagggggg
     4741 caaatgtcaa gataatatgg aacatcaacg gaagagcgat acctgtcgct gcaccttgga
     4801 gcaatcgacc aaacaatagg accatgaagg taggagagaa gaaatcgatc attatacctg
     4861 ccaagaaaag cagatttgaa gctaaaaaga gttttttgag actaaaattg cgattcaaat
     4921 aagtagacag cggaacaatg atcgaaatca tcaaaagata aatcgttgtt acccattgga
     4981 cttgtgcagt cgttaaatca aactgtctga taagagttgg gaaagttaca ttcatggccg
     5041 tctcgatcaa tacgcccgca aaagacatca tccctgttgc aattactgca ggtactaaaa
     5101 gcagctgttt ttgttttgtc atttacatca cctctaaaaa atttcgtaaa aaaagagagt
     5161 gatttctatt ataaaaatag aaaccgcctc tctctcgaca cgccgaaacg tgttatctgt
     5221 acgatttatt tacttcactt attctagcac aaactgaaaa atagtgtcaa cacgattagc
     5281 caagtcactc aaaaagaggt atgataataa ataactaaat ggaatttcgc ggaggaagaa
     5341 aaatggaaaa aattgaatgg cgtaaagaca agagctatag tatgaaaaaa agcccgcaaa
     5401 tactagaggt tcctaaaaaa ccattcttta gcatcagagg gatcggcgac ccgaatcagg
     5461 aagactttaa acgaagagta gcctgtctat atgcagtcag ctatgcaata cgcatgtcgc
     5521 ctaaaaaaaa ttggctgatt cctgattatc agatgtatac tgtctatcca ctggaaggac
     5581 aatggggact gcaagaaaag tatttgcatg aaccagtgat gaaaaaagaa catttttctt
     5641 atcaattgat gatccgacag cctgactttg ttacagaagc gatagcacaa gaagccattc
     5701 agcgttctag caaacttccg gaagacttga aagaacaagt agtattcaaa aagatggaag
     5761 aaggtctttg tgcccaaatc ttacacgtcg ggccctatgc cgaagaacct gcaacgtttg
     5821 agaaattaga agcattcatc gcaaaagaag gctatgaaag agcttctaaa gaa
//
LOCUS       DACDPS010000110         5701 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.110, whole genome shotgun sequence.
ACCESSION   DACDPS010000110 DACDPS010000000
VERSION     DACDPS010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.110"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            93..485
                     /locus_tag="IWJ07_002472"
     CDS             93..485
                     /locus_tag="IWJ07_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAP9334005.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            561..1496
                     /locus_tag="IWJ07_002473"
     CDS             561..1496
                     /locus_tag="IWJ07_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAP9334006.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            1629..2408
                     /locus_tag="IWJ07_002474"
     CDS             1629..2408
                     /locus_tag="IWJ07_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAP9334007.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            2401..2718
                     /locus_tag="IWJ07_002475"
     CDS             2401..2718
                     /locus_tag="IWJ07_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP9334008.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            2962..4572
                     /locus_tag="IWJ07_002476"
     CDS             2962..4572
                     /locus_tag="IWJ07_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9334009.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            4729..5577
                     /locus_tag="IWJ07_002477"
     CDS             4729..5577
                     /locus_tag="IWJ07_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9334010.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         1725 a          973 c         1176 g         1827 t
ORIGIN      
        1 gtttgatttg atgcacagta taggcaatgt tgtgttcttt cttgtttttc tgccagtcgt
       61 caaacgtttt tacaatcatt caggaggaaa aaatgaaaaa aataatttat agtttgcttg
      121 caatgagtgt cagtatactc attttgacaa gttgcagtac aacctataca caatcggctt
      181 ccaaccaaaa ggaggaagta aaaacatcgc aaaaagcaac aatcacgtta caagaaaatg
      241 gtcaaaactt ttcggaaaaa gaagtgttct tcaaaaaagg cgatgattta ctgagtattt
      301 tgaaacgaaa ttttgaagtc aaagaagata acggcttcat cacttcgctt gaagggcact
      361 cacaggatga gaaaaatcaa ctctattgga tgtttactgt ggacgggaaa agtgctacta
      421 ctggtgccaa agagatcaaa ttgaatgatg gacaatcggt cattttcaat ttgtctaaac
      481 tataagtttc ataaaaaact gttaaagggc gaatattgcc ataattcagt gagttcagtt
      541 gatgaaagaa ggaaaataac atggaaaatt ttaaaaaaag cggaatgttt tccgtaattg
      601 ctgctctagg ggctaatatt ctagttgcta tcagcaaatt tgtcggctat gcaatcagtg
      661 gcagtgccgc catgctaaac gaaagcatcc acagtatcgt tgattgcagc aatcagattt
      721 tgctgttatt cggtgataaa caagccaata aaggacagag cgagttgcat caatttggtg
      781 aaggacgagc aaaatacttt ttcagtacaa tcgtcgcaat gatgcttttc ttcggcggtg
      841 gggcacttgg ggtgatggaa gcttttgaaa agttgacgca tccatcccat gaagtgggta
      901 atccgatcat tgttatagca atactgttat ttggtttggt aatcgaagga agttcgttac
      961 gcgtggcaat gaaagaaatc aaagaattga atactgaaaa actttccacg atgcgctttt
     1021 tgcgcgaaag tcgacacagt gaaatactga ttatttttac ggaagacttg tgtgctgtaa
     1081 tcggtttggt cttggcttta gcaggaacaa tattgactta tgcaaccggg attgctgcat
     1141 ttgatgcgat cagcggattg ctgatcggtt tcttgctgat gggagccgcc attttcctag
     1201 caaaagaatt ttatagtctg atcatcggtg aaagcgtgac agcaagcgat ttatcaaaaa
     1261 tcaagttagc ttttgaacgt cctgagatca gtcggctgat cgatataaaa acagttcatt
     1321 taagtccaac ggagatttta gttgcagcaa aagcagatat cgttggtaac aaagagaatg
     1381 aagcttactc ggtcatcaat gatattgaaa aaaatatgcg tcagtctctt cctgacaaga
     1441 agatgtatat atacattgaa acagataaat atgacccaaa ttattctcgt aaataaaacg
     1501 tttattcatg aattgaagca aaaatgagaa aaatataatc ttgtttttaa ataaaacggc
     1561 tacaatttag cgaatttatg ttacaatgag tccgctgttt atttgttgta attaaaggag
     1621 atattttaat gaacaaaaag aaaagtgcgc catggcttca agctttgcat gcagtgatgc
     1681 cactttgtat aagctatata ccagtaggat tagcttgcgg agtattgttg caaaaagttg
     1741 gatttgatcc tctcttatca ggactgttat cgattctggt cttttccggt ggcgcgcagt
     1801 ttttagttgc atctatgctt acgacgcaag catcatttgc aacgacatta ttgatggttt
     1861 tctttttaga attacgctat acattattag gagcaagtct tgccggtttt atgaaaaaag
     1921 aaaagcggac gtttttggct gtcttttctc aatcactgaa tgatgaaaac tatgcggtga
     1981 actacttaaa gttttcaact gacccttcct ggaataaaag aaaggcttta tatgtcaatt
     2041 ggttctcaat gactgcatgg gccggtagca atatgatcgg taattttttc ggctcggtga
     2101 ttcgagtaga tgctgacctt gtccattttg ctttgacggc catgttcatt ttcatgttta
     2161 tcatgcagat gaaaagcgca ctactgatct ttacaggttt attctcaggc gtattagggg
     2221 tcgtattcat ggtattattc caaaatactt tcggattgat tgcagcaaca gtcattgctt
     2281 catttgccgg atttatgctg gagaaggcat tcaaaaaaga aaaagctaag aaactaagag
     2341 accgcggtaa agatgtcaat gagccgctct tcaacttccc ggaacaggag gccaatcacc
     2401 atgactaaac tatatttgct acttttagtt gcttgtctgt tcatcgtggc atatatcccg
     2461 cgcttgttcc cgatgcttta ttttactcat cgaaaagtgc catcctggtt cagtgactgg
     2521 atgaaatatg taccggtggc attatttgcg gcgttagctt ttaaagatgt tttcattact
     2581 catgaacatc tggatattgc ttggaatatc aaaatagctg caatgattct agtagcaggc
     2641 gtggcttaca aaacacgttc gatggcgttg tcagttttga caggacttgc gtctgtcttt
     2701 ttgctgtcta tgctttaaac tgttttattt tattaaaaac agagattgcg acataagttg
     2761 ttcaaactga tctatccgaa ctgcatttgc aacagtcaat tctggatgca agtttattcg
     2821 tagggataaa aaagattagt gttggcttat gatgcaagct cttttttatt ttaggaatta
     2881 gagagggatt tgtccttcta tgattttttg ctataatagg gaagatgttt attttatgaa
     2941 agaatagttg agggaattta aatgaataaa aaattgatgc aaggaacatt ttggctaacc
     3001 tttgctaatc ttctatgtaa agttttaggg gtagtctatt tgattccctg gctaagtatg
     3061 atggggaaca atcaagatgg tatgcttgct acgacattgt ataatgttgg ctatcttcct
     3121 tatggattgt tcctgatgct tggtacagtc ggatttccaa atgcgattgc aaaaaaagta
     3181 gctgtagcca caaaagaagg agataacgct gcttgtcggc tgatttttag aagtacgatc
     3241 aatatcatgt ttgtcatcgg tatcgcctct gctgcactga tgtatttatt tgctccttta
     3301 ctagccggaa tcagtccgat tgctaacgtt aataatggga tactagctat cagaagttta
     3361 tgcccatctt tgattgctat cccaattctt agtgcaatga gaggatattt ccaaggaaaa
     3421 aatgatctgc ggccttatgg aacttctttg attatcgaac aggctgtgcg agtgatcgtt
     3481 atcttggctg gtacttatta cttgcgcgta ttgactgacg gaaccatatt agaagctgta
     3541 ttgatcagta cagtcgcttc tttcttcggt gggttagctg cgattatcca tatgtatatc
     3601 gtcggacgca gaaaagactt cttcgaactt aaagattttt tgatttccag ccgttatttc
     3661 gaaagagaaa accgatctgc ctctgtctct atcattcggg aaacactacc gtttattttt
     3721 gtcggctcag tcattactat cgttcaaatg atcgaccaag taacgatgaa accactgctt
     3781 cactttttcc gtccagagat cgcagatcag caactggaat tcttattcag ccgtgcttct
     3841 gtcaatccaa ataaattgac attgatcctg atctccatgg taggaacagt agctataagc
     3901 agcttgccaa tattaagcac gatgaagaaa aaagatcgga tacaaatcga aaaaacagta
     3961 ggagatagtt tttcgattgc gttattgatt cttcttcctt cattgactgg aatgtcttta
     4021 ttagctggac cactatacac attgtttttt ggctatgatc ctgaaagtgt cggatatttc
     4081 caaatggctt tgctagcttc tttgtttttc tctttattca cgatcctatc tacgatgctt
     4141 caatctttga atcaccatcg ttttgcgatc cgattaactg tagaagcaat tatcctgaaa
     4201 gtcatcttcc aagtaatcgg attaggatta gttggcggat acgggatgag cttatcaaat
     4261 ggcttagcat tcggtattgt ctttgttcgc ggctatcact ttatgtgcaa agaataccga
     4321 atagcacctt tagcaaagat cagcaatttt ttcttaaaaa ctttccgctc caccctgatt
     4381 atgcttgcag tatgttttgc cgttttcttt ttgctgaatc agcgattgtc aatggaatca
     4441 aaaagctatg caatgattta ttgtgtggct gtaggaagta ttggcggctt agtattttta
     4501 ttttctcaat tcggaaaaaa tgggtttcga atgttgaaaa atgttaaaca tcatagcaga
     4561 agcaaagaat aggttaacta atcgtttttt gctgtcactt gaaaaacggc aactcgtatg
     4621 attacgcttg tcgtttttct attttccaat tttagtttca tatctaatct tacttatgat
     4681 atagttataa ggtctggaaa aattctttga aacgaggaaa ttaaacacat gaaacttgca
     4741 gtagttacag atagttcggc atatctgccg gaacgtatca agaaccatcc cgacttattc
     4801 gttattccaa tccctttgat tatggatggg aaaatttata acgaaggaat cgatatcgaa
     4861 gcggatgaat attatggact tttaaaaaac agtaaagatt ttcctactac ttcacaacct
     4921 gtcgtagggg aagttttggg tttatatgag gaattgaaag agaaagggta tgacacgatt
     4981 ttaagtatcc atctttcttc agggatttct ggctttgtca acacattatt tgcgatgaaa
     5041 gatgacatca gcggagtaac ggtcattcct tatgactcta agatcactag tatgccgatg
     5101 ggacacatgg tagaagctgc cttagactta aatgatgaag gtcagtccct tgatgaaata
     5161 cttgcacata tcgatcgaat ccgagataat acctacgctt atctgattgt agatgatttg
     5221 aacaatttag tacgaggagg acgtttgaca aatggtgcag cgctgattgg cagcttatta
     5281 aaaatcaaac cgatcctgac attttctgaa gggaaaattg ttttattcga aaaaatccgt
     5341 tcaagtaaaa aagcatttgc tcgagctgaa gaaattattg gtagaagaaa taacgaaata
     5401 caaccgccag tcaaacttta cgtgatacat gcaaataatt tagaagtagc gattgcggag
     5461 aaagagaaat tgcagaaaaa atatccaaat gcagaaatcg aaattggtca tttcggacca
     5521 gtcattggta cacatttagg tgaaaaagcc atcggcattg ccatttcagc gcaataattc
     5581 cttttattca aaaaacatac caaaaatatt ccaacatcat cttggacttt ctacatgtta
     5641 ctaatgagcg tgtaaaagaa cgagatggtg tttttatttc tatcaatgaa gctctttttc
     5701 t
//
LOCUS       DACDPS010000111         5554 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.111, whole genome shotgun sequence.
ACCESSION   DACDPS010000111 DACDPS010000000
VERSION     DACDPS010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5554
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.111"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(211..714)
                     /locus_tag="IWJ07_002478"
     CDS             complement(211..714)
                     /locus_tag="IWJ07_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP9334011.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1073..2098
                     /locus_tag="IWJ07_002479"
     CDS             1073..2098
                     /locus_tag="IWJ07_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9334012.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2421..3299
                     /locus_tag="IWJ07_002480"
     CDS             2421..3299
                     /locus_tag="IWJ07_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334013.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            3775..3891
                     /locus_tag="IWJ07_002481"
     CDS             3775..3891
                     /locus_tag="IWJ07_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP9334014.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            4819..5157
                     /locus_tag="IWJ07_002482"
     CDS             4819..5157
                     /locus_tag="IWJ07_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334015.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5251..5481
                     /locus_tag="IWJ07_002483"
     CDS             5251..5481
                     /locus_tag="IWJ07_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334016.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
BASE COUNT         1867 a          839 c         1027 g         1821 t
ORIGIN      
        1 ttaagatttt aagcctttaa gctaaaaaaa aacccttttt gttaaataag gggaatatag
       61 cctgtcagtt tcaaactgca agtcgtcctg ctgcaataat atagtagatt agagatgaaa
      121 atagattttt ttaaaccaat gactgaataa aaagaccgtc atagatacac attggaatgt
      181 gtctattacg gtcttttttt acgaacgatt ttattgaaga cggacagttc gggtcaaaaa
      241 taaaaaagct acgatcagaa ataagaataa aatgtatccc aaaataatgg ctagacggtc
      301 taacttattt gtcgatagat gtttgtttag aagttgatag gcccataaac ctatcagtcc
      361 tcctgttgta ttagttatca aatcagtgat gtccgctacg ccaataccga ataggaattg
      421 aatcaattct gccaagaagc ttacaaggaa aataatccct aattttttag aaaaagaaag
      481 tctcttgaaa ttgacattca aaagcacccc aaaaggaaaa aagacaatca agttataagt
      541 ggtctcacgt aataccgtcg tattttcaaa agaaaaagta gaaaaaggga cgagattgac
      601 attgctgtaa gaataagtga gaaattgtgg aagattaaat gatagcttga ataaaatcaa
      661 ccataataga aagaataaat aaatccctaa taaaaatttt gatacatacc gcatagacat
      721 ctcctccatt taaaaagtat accatcgaaa accgctgaaa aatcataaaa aagtatgaag
      781 tttaaggacc aacgtagtta tcgtacgaaa attagaagct tttttttgtt gggaatccgg
      841 tttctttaac aaagctaaga tagcgagttg cttggaaaag ttgctatctt ttttctgtca
      901 aaacgactcg gaatgttcgt tttttgttct aatttctcca agttgtttac tttttgatac
      961 taagtttatg aaaaatcaag aaatagttgt tatactaggt aatgctgaca aacgggattg
     1021 gcatttcatc ttggttcagc tttttttcca attactagga aggagggata gattgttcaa
     1081 gacttatttt cgtttcaata aaatacaaga taatcctttt cgtatcatga atctgatgct
     1141 acttatcttt attgtattat ggttaggtta cataaatcat gattttttga tttcaagttt
     1201 ttcatcatta gggatcttta cttttttcta ttatcaaaac ataccgatga aatatttgct
     1261 gaaacgtatg tttctgatcg gttgcggact gttggttgct ttcgttttgg ggatcttatc
     1321 gacttatgtt ttatggcttg aaccatttgc cgtagctttg gttgcttttt ccagcagatt
     1381 cgtgttgcga ttattccata tttctaaacc aggtggcttg ttttttgcga tgctttctgc
     1441 catgggaacg agcatgcagc tgccaatcgc tcaattaccg atcgtctcac tttatttttt
     1501 tatgggtgtc gtttttgccc tgattgcggc agtgattaca aaattactgg attcacggcc
     1561 tgagcagaca attgaaaaag caactttaaa agaaagattt cacgaagagc ctttagtgat
     1621 cattgattct gttttctaca gcgctgcctt atttctttcc gtttatgtca gtcacggctt
     1681 gaatctgcat aatccttatt ggttgacctt gtcatgtgca tcgattttgc tagcagaaaa
     1741 cttggatgcg atgaagcatc gacaagtcca atatctgatt ggatcgatgg gtgggttatg
     1801 tgtgtcggca ttcttgtcat ttgtgccgtt tacgcagttg cagaccattt ttctgatcac
     1861 ttttctatat ggaatcgcac aatttctagt tgctagaaat tatgcagttg ctaatatttt
     1921 tcttaatccg atggcgttga tgctatctac acttattcgt ggagcatact tgatttcttt
     1981 gatcgaatat cgttttttag ggatcgtcat aggaagtttc atcggtttag gtgttgcgtg
     2041 ggtaatgacg gttggtttgc aacattattt aacggttgtt cgtcaaaaat tagaatagaa
     2101 ggacaaatat atgtgccaaa agaggttatg acaagatttt tgtcacaacc tctaattcaa
     2161 ggtgttcaaa agccagattc tcagcattaa gtcaaattca cacaaaaatg tggaaaggaa
     2221 ctttcaaaaa aattttatat ttattctatc aaaaaatttt gtttatgatg gtaactgaat
     2281 aaactttcct gagatggtca atgctttcct tgaaagtctt acagatattc tttcttcata
     2341 gaaagtttaa gttttatagt tagtgtacaa taaaagtatg tttataccat atggtgtccg
     2401 gataatcagt catttaaaca atgaggttca aagatatgaa aaggggaaat ttgatgaaga
     2461 aaataagcaa tgaaatccaa attctggcag aaactttaac tcttcccaaa gtcgttatag
     2521 agcaaatgat tgtacaagca aaaaacatcc ttgatgaggt gtttgagcaa tatagtcatg
     2581 aactaatgga tcctaataaa gcaaaggcga ctgcaaagaa aatagacaaa gtctatgaag
     2641 aggaaccttt tgcagcatta gctatttatc tatatgcagc ttctcaaagt tggaaaaaaa
     2701 tttatcaacc attgaagatt ccaagaacta tctatttggc aacaatgaat gcttttacta
     2761 gatttattca tgaacatttc caagtaacag gaagctatcg ctttgaccgt ggtttttgga
     2821 tttggcatta cactagtggc ttgatctttc ggattggtga attggagtac gaaatgacat
     2881 attttccgaa agggtacacg gatctaagat tagaaggaaa gcagtgcctt tctcttcata
     2941 ttccttccga tgcagattta tctcagaata agataagaga tagttacgaa agtgccattc
     3001 gtttttttga actttatttt cctgattacc attaccaagt gatgtacaca gatacttggt
     3061 tactatcacc caatcttact aaatggttga aaaaagagtc aaaaatctgt ctttttgcag
     3121 ccgattatcg attattatca gttgatgaac aggacgatag tggagtccct tggatctttg
     3181 gacgagtaga tgcccaaata catgattatc cagaaagtac gagtttacag cgacaagcaa
     3241 aagagcaatt gcttgcaggc gagcacattg gctctggctt aggtattctt acttcataag
     3301 tttagtactt cgaaatagtt taaaagagcg tgagacaaaa gtaaagaaca cttttgtctc
     3361 acgctctaaa cacgtataaa cggcggaagc agaagcaact ccttcggaaa taagctgaaa
     3421 ttcacaaaaa tttgaaaagc aattttcgtg aatttcttct tatttcttgg agttaaacgc
     3481 ttctgtccca gcctcttttt tagtgttcta tcgaacacac agtacgttca ataggtaaac
     3541 tagctataac aatagatatt aaagttaacg gacaagaggg ccttttttag gtagctaaaa
     3601 cgtaataatg aaaatttatt tccagagata ggagtcaatc agctagtctg atcgactgtt
     3661 ttaacaagtt gactttcatt ttaacttttg ataccattta aaacacaaat cgtaattgtt
     3721 tcgatttgtg ttttttagca tgactacaat caaagaagaa agtgggtcgt ccatatggaa
     3781 aaaacagata tctcaagtgc ataccgccgc ttgaaaagcc caaatatcaa aacacgaaaa
     3841 cgtgcgctga agatcatcaa agatgtgaaa cggaatagtg ggaaaaggta agaagaagga
     3901 aacttaatta aaaaaatagc cgaaaaacgt ttttcggcta ttttcaataa tcggtttttg
     3961 tgtaaggttt ccgaaagaac tgctgttgat tttccatttt taacatatga taaattggta
     4021 gaatctttct tttcaaattg ataccattcc ctaattccag ttatatagtt aactacagaa
     4081 ccacaaagca cgtacgttta taaaagtatt atctgtagtt atttttcaat tccctttcaa
     4141 cctcttattt tgaccagtag tattgatcac tttacctttt aacacctttg ttcgtttttg
     4201 aaacattttt agtcaagaat ggaaacgtaa ctacataatc aaaggttttg cgaaatattt
     4261 catgaaatat ttcatgtaga agcgagcaat acggttgcaa ataaccatta tcgtttaata
     4321 agcagttacg aaagctccca atatctctaa cgttcagctc actccatttc ttttcttcaa
     4381 aaaaatcagt gattcgtatc tctttctatt tgtcgaaaaa gttaacttta aaaagtaacg
     4441 ccgaaaaaat atgcaaaaaa ataaaaaagc agttactatt gctagaataa catcaaggaa
     4501 taataagttt tggttttcag ataatagtcg ataagttgtg tattggaatt ttagatacag
     4561 taataaagta tttttcatga aattttgtga gaatcactat tacaagtatc tttaataaaa
     4621 ttaatggtta aggaggaact taaagtagaa tgtaaatgaa tagttgtagt cagtcgattg
     4681 tgatactaga ctcaaagttg caggaataag tttttcaatt tgtgattgga agataaaaaa
     4741 tgaaaaagaa gggctttttt ttatactttg ttgattgtag tgtttatttt ccctctgttt
     4801 agccaagtaa cattttctat gactcaacca gtttcaagtg aggaacatga taaccttttt
     4861 cgtgctacag aagagtttaa gaaatatcag gaaaatattg ttccggagac tccattagct
     4921 agtgagccaa ttactgatat tgatggaaac caaataggat ggtatataca gtacgaagtt
     4981 atggtggtaa ataatgactc aatagtaaaa aatacagtag gcttagagct aacaccgatt
     5041 gaaaaggtga gttcattgtt aacgtatatt tatactgatg aaagtgacga gactgagatc
     5101 attttaacgg attactcaaa aattgctact gataaaaagt atatattgtg gatgtagaca
     5161 ctagtgaaat actttatgat atagaaattg atgaaaattt actagatgta gcacaagaaa
     5221 tcgatcatca aaagcaggaa gctattcgat atgctggtag aaaagggaaa agaaaatatt
     5281 tattacgtga atgtagcaaa agttagagaa gatgaaaatg agtggaaaga attcaagagt
     5341 cgctactcaa ttaactcgac tccgacattt acagtttaca gagaaggatc gatagaaaaa
     5401 actgtttttt ggactaaaga aagtggaatg tctttagctg aagtagaaga gtttttggat
     5461 tatgttagta tgcaacagta agggagttct tattatgaaa aattttgaca catattatgt
     5521 tattgttttt tcgatatctt tgctgttaat atta
//
LOCUS       DACDPS010000112         5542 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.112, whole genome shotgun sequence.
ACCESSION   DACDPS010000112 DACDPS010000000
VERSION     DACDPS010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5542
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.112"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(378..1085)
                     /locus_tag="IWJ07_002484"
     CDS             complement(378..1085)
                     /locus_tag="IWJ07_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334017.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENG"
     gene            complement(1150..1986)
                     /locus_tag="IWJ07_002485"
     CDS             complement(1150..1986)
                     /locus_tag="IWJ07_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9334018.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(2004..3413)
                     /locus_tag="IWJ07_002486"
     CDS             complement(2004..3413)
                     /locus_tag="IWJ07_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9334019.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3427..5250)
                     /locus_tag="IWJ07_002487"
     CDS             complement(3427..5250)
                     /locus_tag="IWJ07_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9334020.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
BASE COUNT         1683 a         1216 c          903 g         1740 t
ORIGIN      
        1 gatacatatg aaacaattac atatgatttt ttcataaaaa aacagtactg tctagtagta
       61 taaaatatac aaatttctta aaaatttttt cactaaaatc actacctgaa acagtctaaa
      121 acacttatat atcaacgttt aatgaaacta tctccgtttc atttcagata caaataaata
      181 tagtcgggct ccaaatccga aaaaaaatac ccccagtagc ttatcctaag ctactggaga
      241 tgtataagta cggtcatata acaacggggt agggtaaata aatgaaaaag atggaagaat
      301 cattattcaa taactttttt aaatacttcg ttgtaatcag ttacctgtcc gttcttaaat
      361 accacagaat aatcttttta cccattttca agtaaccgaa gtggaatgtc tttaccaact
      421 tgctctgtca ctgcattact gatacctgtt agattagcta ataaatctga taaagattga
      481 gcatctgtag aaatctctgt atcagtattt atcgtgattc ctttatcttc atttacaagc
      541 ttccaagtag gtggtatagc tgtgtatgtt tcgatgaatg aatcaattct ttcatctatc
      601 gtcttcccag tatctacttc tgtctcatta ctcttagatg aatcgattct ttcatccatc
      661 gtctgctccg taactgcttc tgtctcatta ttctcaccgg aaactctctt cacacccaaa
      721 cttatatatt gtccatcttt agtgtgcata gttaaaattc ccacgctttt actaataaat
      781 tcactataac cagttctttc aatagagact tcaatgtatt tcacactatc atactcaaaa
      841 gaaaaataga catgtaaaag cattttgttg tttacatcta cataataatc ctttgactct
      901 acttgagacc ttcctatacg gaatacagct ttactgtctg atatctctat accagcatcc
      961 ttattattaa tctgccaatc tccaagtagc tcttctggca agctagtagt ttcttgtgaa
     1021 cactttacca cttcactggt agaggtcgaa acattcacct ccgcactgtt agaagaattt
     1081 tccaaggaat caggagcctt agcacaacca acaagtcttt atatattgtg cagccgatat
     1141 tttcttcatt cagtacaccc ttgaaatatg cagtgttaga taaagcagtt cgtcctgatt
     1201 tgctgaggca cccatctctt cttgaacgta ctccttgatt ttcaaggccg cccgatactc
     1261 tttcggaaaa cgctgcttca ctgaagccag caaaaaatca tccttctccg gcaactcttc
     1321 cttcttaatc attcgggcaa taaaaaactg taaatgcgtc acaaatctta agtaaagcat
     1381 cgaatcttta tcaacctttt tttcgggaaa cagatatcct agcatagcca agatatcctt
     1441 tagtgctctt accgagagca tggtttcttc catatttgct tgttcacttt gggcatttac
     1501 aatgtgaaac gcaatgtttc cagcctcctc ttctggcaac tgaacattca tttttttgtt
     1561 gatcaattgg atagcatagc agccgatctc aaattctttt gggtaatact tttgaatttc
     1621 aatcagcagg cgattttgaa aaacaatgcc tttttgactg cgttcaatgg catgcttcaa
     1681 gtgatcaacc aaggtaaaaa atagactttt attcagctta aagtcaaact tggcgttggc
     1741 ataatcgata attcgcttga cgcaaataat tagatcatcc ggagcagcct caaacagttt
     1801 aatgtaatcc ttttttagag catcagaaag tgaatctaat ttaaatactt tttggatttt
     1861 gtcagccgga accaaatcgc ctttctttag ttgaaatcca atccctttcc ccatcaaaat
     1921 tacttctttg ccttgcttat ctaagcaaag aattaaactg ttgtttaacg ttttaattac
     1981 tctcattaga ttcaccgttc tctctaagct aagccatttc ttgcgattac tttttgatac
     2041 caaaagaagc tttctttttt tattcgtttt aattctttaa gattctcatc ggttcggtca
     2101 acatatataa aaccataacg cttattgaag ccttgatggg tactaacaac atcaatcgct
     2161 gaccaaggta aataaccaaa aacatctaca ccatctgtta aagctgcttg aatagccaga
     2221 atatgtttgg ctagataatc aattcgatcc tggtcattaa ttcgtctatc ttcctcaact
     2281 atgtcgggtg ccccatagcc attttcagta attaaaatag gtaaatcata gcgatcgtac
     2341 aatttcctca atgttaatcg tagtccagta gggtcaacta cccagccata tggcgtttta
     2401 tctacataag gattttcagc tgcccggtag acaccttctt ctcccaacat gatctgttga
     2461 tcccccgctc tagcggaaac atctgatccg tcatttttgc tggcagcgat ggtggcagtt
     2521 gaataataat taatcgcaat aaaatcgggt ttaccggagg ataaaattgc ctgatcctct
     2581 gcctcaattt taggatataa tccacgatca ttcaaatagt tttcaaacaa ataattgtat
     2641 tttccccgaa ctgctacatc taagaaagac cagcctctca atgtttccca attatgggct
     2701 gcaattgcat cttctggact ggaggtagct tgatacatag ccgttaaatt gattgctgga
     2761 ccgatttttc ctttaagatt caactgatga tacaagtgaa ttactctagc ttgagctaat
     2821 aaaacatggt gattaatttg aaagagttct ttttttgaag gcagctctcc acttggtggc
     2881 aaaccaatcg ctccaggatg aaggatcatc gtgttttgct cattaattgt cagccaatag
     2941 ttcaccttgt caccatactc agtaagcaat acctgtacat agtccacaaa ataatcgata
     3001 gttgctcgat ttaaccaacc gccctgctcc tctagaaaat aaggtaaatc gaaatggtac
     3061 atggtgacaa tcggttcgat tccctgattc aggcattcat caattacttg atgataaaaa
     3121 gaaattccct tttgattggt tttattattt tcaatatcca taattcgcgt ccaagcgatg
     3181 gaaaagcgaa aagcttttaa gcctaattct ttaaacagct gtatatcttc tttatagtga
     3241 tggtaaaaat ccgaggcaac ttcaaaatta gctacccctt ctgtatgctg atacttgtca
     3301 ataatagacg gttgtttccc atcctcctga taggcaccct ctgcctggta ggcactaatg
     3361 gaggcacccc ataaaaactt ctctggaaat gctgaaagct gtttgtattg cacaaaaatc
     3421 ctctccttaa ctaatttgca tcaatggttc gtgttccttt aagcttccgc ttgcttcact
     3481 aattcctgcg taattttcac tgttggtcac taccacaatc actgtatcat cgtagccagc
     3541 gtttctaatg gcagcgaaat cggcgttgat taataattgt cctgctgaca cctgatctcc
     3601 tgcttttaca aatacttcaa agggttcccc ttttaaattc acggtgtcga tccccacatg
     3661 gatcaataat tcaatacctt ctttagaacg tagaccaatc gcatgtttag attctaaaat
     3721 agtaatgact tctccctcaa caggtgaata aatgctgttt tcttctggat gaatggctac
     3781 gccctttccc aatagtccct tagaaaagac agcatcgttc acttcagaaa taggacacac
     3841 ggttcctttt accggatttt caacaatgac tgcttcggct attgccgttt gctctgattc
     3901 atcagaagta tcgtcaaatc caattagata agcaatgata aaggttgcaa caaaggcgat
     3961 tacacacgta ataattgcgt gaacaatatt cattggattt tctccaataa aggctggtaa
     4021 ggctgctaag ccaggggaaa caaaggcata tctgactaaa ccgctcaagc cagcatacaa
     4081 accagcaatt gcaccaccaa tcatggaagc aactaatggt tttttcagct tcaaggttac
     4141 tccatagagt gaaggctcag taattcccat taaagcagtg atcccagtgg acacagctag
     4201 ctgcttcaac tctttgtttt tagttttcgc ggctacagca aaggtagcac ctgattgagc
     4261 aatattagaa gccagcattc ctggcccatt gatcatttca gaacccgtac tgctcaattg
     4321 catagtagcg attggcgtca ttgcccaagc tgtacccgta accaccaaaa atggctgcaa
     4381 accacccatc aaaaatggaa ttaaccaagc tgctttgccg ttaatccatt cagcccccat
     4441 tgctataaga tcgttcagca acgttccaaa cggtccaatg acaaccaacg ctaaaggccc
     4501 cataattaaa aataccagca tcggcttagt aaagaattta ataaacgaag gcgaaattcg
     4561 atccgcaaag ggctctacat aggacatgat ccaaacagta aagataatcg gtaaaactga
     4621 tcccgcataa tctgcagcta ataccggaat tcctagaaaa gaaacggcat cgccagtaga
     4681 aaaaagctgt gaaatatttg gatgaagcaa aatgccagca acagtaatcg ccaaaattgg
     4741 gttgacatca aactttatgg ctgccccata ggctaatagt actggcataa aataaaaggc
     4801 tgcatcagca atagtattca aaatgatgta agtttgacta ctgtctgata gtacaccagt
     4861 caaagtcaag acggccaaaa tggcttttac catcccggca cctgttatag ctggaatcat
     4921 cggtgtaaag gtggtagaaa tgacactaat caatcttgaa ataatacttt cttgattgtc
     4981 tgaagagggg ccctttttaa ttgaagttcc cacttccttt tgaatttcct tgaaggctgt
     5041 ttggacttca ttgccaatca ccacttggta ttgtccaccg ctatccacca cactaattac
     5101 accaagtgtt ttttctaagg ctttcgtatc ggctttcctt ttatctttga gttcaaaacg
     5161 cagtctggtg gcacaatggg ttaagctgac cacattttct tttcccccaa cctgttgcaa
     5221 tacatttctt cctacttctc tgtaatccat tattctctct ccttaaagca gtaaaaaaga
     5281 ccaagtcaaa ccacgcccca atcaacattg aagtgttagt ttgtcttggt cttgcctgca
     5341 taatcagtaa catgccaata acgttatttt gctgtaatcc aataatagca cattatgata
     5401 gcgcaatcaa cagggaattt aattttttat cttttcgttt gaccgtatag ggtaatataa
     5461 cagttagatc acatcaacta gggtatatct gcgtatactt actgctaaaa taagtatatc
     5521 taatattaac tataccagta aa
//
LOCUS       DACDPS010000113         5533 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.113, whole genome shotgun sequence.
ACCESSION   DACDPS010000113 DACDPS010000000
VERSION     DACDPS010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.113"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            131..619
                     /locus_tag="IWJ07_002488"
     CDS             131..619
                     /locus_tag="IWJ07_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334021.1"
                     /translation="MELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTAAEL
                     QKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSIQFP
                     QLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVAMEK
                     AN"
     gene            678..1118
                     /locus_tag="IWJ07_002489"
     CDS             678..1118
                     /locus_tag="IWJ07_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334022.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1130..1798
                     /locus_tag="IWJ07_002490"
     CDS             1130..1798
                     /locus_tag="IWJ07_002490"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP9334023.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1862..2485
                     /locus_tag="IWJ07_002491"
     CDS             1862..2485
                     /locus_tag="IWJ07_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAP9334024.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2541..2927
                     /locus_tag="IWJ07_002492"
     CDS             2541..2927
                     /locus_tag="IWJ07_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAP9334025.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3140..4531
                     /locus_tag="IWJ07_002493"
     CDS             3140..4531
                     /locus_tag="IWJ07_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAP9334026.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            5142..5510
                     /locus_tag="IWJ07_002494"
     CDS             5142..5510
                     /locus_tag="IWJ07_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP9334027.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
BASE COUNT         1833 a          917 c         1171 g         1612 t
ORIGIN      
        1 atatgttcaa ttgcaggaaa ataaaacatt gctgcatttt tcaccagagt tccatctaga
       61 ggggctaaca ttaggagaaa tctatcaagc agaagggctg tcggctgttt tgcacttttt
      121 taaggtagaa ttggaattac cagtaaaaga ttatatcgag ctttctcttg aatcttggga
      181 tcgagcagtg gttgaactgt ttccagaagg tttaatgatc gatacaaacg atgggaagat
      241 ccatctgact gcagcagaac tgcaaaaaca aatgcgttat caaatagatg acgaaaattc
      301 tttttctatt tttagacgtc agcaaaagat tctaaaaagc tttttaaaag tgatcagtaa
      361 aaaacggaat ctgatcaaaa cgacacagct gttgaaaaaa tatccaggtc tgcttcacac
      421 gtctattcaa tttcctcaat tgattacgat gatccatcgt tttatacgta tgcaacagct
      481 accatctaag aagctgtttc ttccattgac ggacacattt cgagtagtca acagagaaga
      541 taaaaagaaa gtgattgtga ttgactttac cagaaatcgg aatgtccttc accaagttgc
      601 aatggaaaag gccaattaag gctttttctt tttggatttt tttgtcataa taaatattag
      661 atgatggagg agataatatg atgcaaacat ggttagaaag gctaatacga tttcagcaga
      721 actatcacaa acttatgcag ggaagatatg cgaaatttga tcagttgaat cgcggtctat
      781 tgatcgctgc gcttgtcttt agtttgctaa gtcgttggtt gccctataga atcggacaat
      841 tgttgttcat cgtctttttc ggatggatga tatttcgttt cctatcaaaa aaaatctacc
      901 ccagactaaa tgagaatcaa cgttatttga aatatcttga tagagtcaaa gaaaaatatc
      961 atcagataaa aaacaaaaag caagtcaaaa tggatcgaaa aagttatact ttttttgagt
     1021 gcccgaattg tcatcaaaag cagcgtgcac ctaaaggaaa aggacgaata cgtgtgacat
     1081 gcaaaacatg tggtataaaa ttcgaaacga atgtttaagg gagaaaagaa tgaaattagt
     1141 attattacgt catggcgaaa gcgaagcaaa ttttgaaaat tactggacag gatggctaga
     1201 tgtcgcctta acagaaaaag gaaaagaaca agcagcagtt gccggtcaaa aaatgcttga
     1261 agccggactt caattcgatg ccgtttatac ctctgttttg aaacgtgcga ttcttaccgg
     1321 acaagtcgca ctagaagaaa tgggtcagtt atggcttcct ataataaaaa catggcgtct
     1381 aaatgaacgg cattatgggg atcttgtggg taaaaacaaa gataagatga aagaaatata
     1441 cggaaaagat caagtaaaaa catggcgcag aggatatacg gaagttccgc cgttaaccgc
     1501 agaaaataga tttgaccgca gatatgatca cttggatccg catttgatcc catacggaga
     1561 aagtctggaa atgacagtga atcggatcat tcccttatgg caagatcatc tagcaccaaa
     1621 actaaaagat cggcaagatt tactagttgt gggtcatggt aatagcatcc gagcactgac
     1681 aaaatattta gaagatgttc ctgaagatca aatggatacg atcgatatcc caaatgcaca
     1741 accaatccag tatacattag cagatgattt gacaattctg gacaaaaaaa tactttaaag
     1801 aacaccttta tctaaaagtg agagatagaa aaaagaagca aaggaatgga acaaaaggaa
     1861 catgaaaaat tatatatggg actttgatgg tacattgttt gatacgtatc ccgcaatggt
     1921 agatggtgca tggcaagcac taaaagattt tggtatcagt atggacaaaa aagaaatcta
     1981 cttcaaaatg aaaaagtatt ctactagtta tttgatcaat gaatcaaact tgaatgccag
     2041 agagtttaat gaattatttc accgatatga aaaagaaagt acggaagttt cccgtccatt
     2101 tccagaaacg aaacaagtac tggaaatgct aaaagacaac ggcggtcgtc atttcatcct
     2161 gacccaccgt ttgacagaat caacatgggg cctgttaaaa gaacatcgat tggcacatct
     2221 gattgaggaa gtagtaggga tcgatcagga ctttcctcgt aaacctgatc cagcatcttt
     2281 aaattatttg atcgatactt ttcatttaga aagaacagac acgatgatga tcggcgatcg
     2341 tcgtttagat atcgaagcag ggaaaaatgc aggagtcgct acttgtcttt acgacatcga
     2401 tcattttctg ggtgagatcc cggcagatta tgtggtagga aatctgaatg aaatccttac
     2461 cttgcagagc atcattaaaa agtagtatcc cctttcaaag gagatatgtt ataattttca
     2521 taaaggagtg ataaaattat atggaaatca gaagacaaaa agaagttgta gaagcatttc
     2581 attatgatat gagattgccg aaccaagaag tagaaacaga tttgaaagta ggcttctcgc
     2641 ctttaaaaga aacgatcgaa aactatccga aagaacatag catcgtagga gcacgcttag
     2701 agttccgttt agtgtttgat acatatgttt tatctggaag tgtgagtcag atcaatcata
     2761 tcctcaatcg gaaaattgaa ggtcaaaatg atgtcacaca agaagaagtg gacgagctag
     2821 tagcaccgtt attcgatatc gtacgccgaa tgtcttatga ggtgacagag atcgccttag
     2881 atcagcctgg ggtaaaattg aattttcaat cagaaaaaca agaatagaca gtaaaaacat
     2941 acctgcgata gattaaagct atcacaggta tgttttttta ttactaattg taagcaaaaa
     3001 gaatgtcatt tcgataaaaa tcgtctaaaa tataaaagtc actgaaaaaa attttttcag
     3061 gaggatggtt gaagaatcat tctcgggaac ctttacttcc ttcgatggac ttgtcattaa
     3121 taatatgagg aggagaaaaa tgacaacact catcacagtt ttagtggttc tagtttttgc
     3181 ggcattatta tatgtgttct atcgtatgca aaagcgccat acaaaatttt caacacgcgt
     3241 ctttacagca ttaggagttg gtatcgtctt tggcggtctg cttcaattga tctttggtgt
     3301 agacgacaaa gtcgttactc aatcaatgga atggatcggg attgtaggtc aaggatatgt
     3361 atcattcttg caaatgctag ttatgccttt agtgtttatt tcgatcattg gtgcattcac
     3421 aaaaatgaaa gtttctgaaa agctagggaa aattagtgcg actgtattga cttctttact
     3481 tggaacaaca gctattgcag cattgatcgg aattggttca acaatgctgt tcggactcca
     3541 aggagctaaa ttcacacaag gagctgcaga aacagcacga atctctgaac tggcaacacg
     3601 tcaggaaaca gttgaaaact tatcgattcc acagcagatt ttatccttta tccctaaaaa
     3661 cgtttttgct gatttagcag gtagtcgtgc aacaagtacg attggagtcg ttattttcgc
     3721 ggcatttgtc ggaatcgctt atctaggcgt gaaaagaaaa gcgccaaaag aagcagcatt
     3781 cttttcagat ttagtcgaca gtctgtataa aatcattatg cggatcgtga cgcttgtttt
     3841 gcgtttgaca ccttatggag tatttgcttt gatgacaaac gttttggcaa caagtgattt
     3901 tgcagctatc gtcaatttag gtaaatttgt cgttgcttct tacgtagcat tgcttgctgt
     3961 tttgattgtc cactcactga ttttgattgg tgcaaaagtc aatccaatca actatttcaa
     4021 aaaagccttt ccggtattga gctttgcgtt tacttcacgt tcaagtgctg gagctttgcc
     4081 attgaatatc gagactcaga caaaagcttt aggagtagat gatgcaacag ctaatttctc
     4141 agcaagtttt ggtttgtcta ttggacaaaa cggctgtgct ggcgtttatc cagctatgtt
     4201 ggcaacgatc gtcgcaccag cagctggagt gaatgtcttt agtttggaat ttattttgat
     4261 gcttgtggca attgtaacaa tcagttcttt tggtgtagct ggagtcggtg ggggcgcaac
     4321 atttgcttca ctgatcgttt taggggcaat gaacttgcca gtagcaatcg ttgggttagt
     4381 tatctctgtt gaaccattaa tcgatatggc acgtacagca gtaaacgtca gcggtagcat
     4441 ggtagctggt atcgtgacaa gtgcacgcat caaagattta gatcgcgatg tcatcaatga
     4501 tgattctaaa gtgatcgatg tgaatgcata gtcaaaaaga ggttgtgata gaagtggtca
     4561 gctttaagaa ataagaagaa gtttcctaaa aatagtttct caaatttttg tgaaatttcg
     4621 gcttatttcc gagaagctac ttctgaaaca ccgtgaataa gaggttgtga aaaagctgcc
     4681 ctgcatcaag taataagaac agccaaacaa aaatagttcc tcatatttta cgtttggttg
     4741 agcttattac cgcagatgca agcttttgaa caccgtttaa gaaagaggtc gtgagcctgc
     4801 cgtttcactc tgagtcacaa agagcaattt caaaaaatag cttctcatgt ttgttgaata
     4861 ttgtgtcttg tgaccgagga gttgtcagga gaacaccgtt taccagaggt tgtgacttgt
     4921 ttttagtcac gacctctttt tttatttatt ccatataaat acacattatt tattccgaaa
     4981 agtgaaagcg caaataacaa aaacaaccaa cttatttatt attaaagaaa agcgtattta
     5041 agtaggacga ttctgttgag agctttcgta gattttccca ttttcctatg gtaaactgac
     5101 aaagttgaga atgttagaac agatcgttta ggaggaaaca gatgtttacg aatgaaataa
     5161 aaatcatgct ttatgtggac gatgtagaaa aaaatgcaac gttttggaga gcagttggct
     5221 ttgcggaaaa agatcgtcaa gaaatggatg ggacgcttat aattgaagta ggagttgagg
     5281 acagtcaaac ctcatttctg ttatatgatc gagcatttgt tgagtcccat tcacctggca
     5341 cagctactag tgccccatct ttgatgtttt acagtaaaga tatctttgga ctatataaaa
     5401 aaatgcagac gcaatcagtc acattaggtg atatggtaca gcttggagaa gaatatgtct
     5461 ttaactttgc tgatcccgat ggcaactact ttgcggttac aggtaaataa tagaaataaa
     5521 gactttgttt ttc
//
LOCUS       DACDPS010000114         5477 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.114, whole genome shotgun sequence.
ACCESSION   DACDPS010000114 DACDPS010000000
VERSION     DACDPS010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.114"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(133..531)
                     /locus_tag="IWJ07_002495"
     CDS             complement(133..531)
                     /locus_tag="IWJ07_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAP9334028.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(528..1301)
                     /locus_tag="IWJ07_002496"
     CDS             complement(528..1301)
                     /locus_tag="IWJ07_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP9334029.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1298..1981)
                     /locus_tag="IWJ07_002497"
     CDS             complement(1298..1981)
                     /locus_tag="IWJ07_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP9334030.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2148..3035)
                     /locus_tag="IWJ07_002498"
     CDS             complement(2148..3035)
                     /locus_tag="IWJ07_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9334031.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3032..3901)
                     /locus_tag="IWJ07_002499"
     CDS             complement(3032..3901)
                     /locus_tag="IWJ07_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9334032.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4038..5324)
                     /locus_tag="IWJ07_002500"
     CDS             complement(4038..5324)
                     /locus_tag="IWJ07_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9334033.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
BASE COUNT         1695 a         1221 c          904 g         1657 t
ORIGIN      
        1 tctgatgctt agagtagaat ttcataaaaa tttactattt cataagtatt cacgagaaaa
       61 atacaaacaa aaaagcggaa ataacgctca tccgccttct tgtttgtgtt tatttcctct
      121 tcaaacgctt gtctatgatt cagacttttc tctatcgtta ttttcatcta aaagttcttg
      181 cattccgata atttctcgat attgatgaat cttagtgata ccaatgactt tgtctacatc
      241 aatactaaaa caaataccat tacctggaat atcgatgttg atgccttgac caatcgcatc
      301 cattacttta ttcgtcaaac tatcgggtac taatgtcaac acaatatctt tttctggcga
      361 aatgtccaaa cctaaaaatt tccctgaatc atgtacacca gaacctcttc catgtaagat
      421 cgttccgcct gctgcacctg cttcttttga aaagtcgata acatcttcgc ccattcctcc
      481 gtcaacgatc gtcacaatca tgtgaagatt caatgctaca agatctttca tttgctttcc
      541 cccaattttt gcaatctttc tttttcgatt ctgtcttttt catcttttcg tttataaata
      601 tatcctagta ctaaagtgga taatattggt gtcaatgcaa tcattgcaac cacaccaaac
      661 ccgtcaatga tcggatctct tccttcaatt tgtgcagcga ttgccacgct cattgataaa
      721 ataaatgtcg aacacatcgg gcccgttgct actccaccgt tatcaaaagc cattgctaaa
      781 aagatcttgt ccacttttcg tcctaaaata aatgctagcc cgtatcctgg gatcaagaaa
      841 taataaagag agaatccgac gagcaaacgc cacatagaca atcctactgc tgcgccgatc
      901 ccaattgaaa ttaccatcag catgattttc gatcggattc tcccctcgct caattcttca
      961 acctgtttga tcattacatg aatcgccggc tcagcaaacc ctactaagaa ccccattaaa
     1021 aagccaaggg gaaccagtat ataattgtaa ggcaatcccg caatagcact gcctaaatgc
     1081 tgtcctaccg gtacataagc catattgact ccgtgcagga aaaggatcaa cccaatagtc
     1141 gttatcaaga aacctttcat aatgtcaaag aaacgctttt tgcgcatacg aaaactaatg
     1201 atattcatta aaacaaatag gattacgata ggtgccactg ccaaaatgac ttccatggta
     1261 acgatttcga ttccatctat cacttgctgc cagaaactca ttggaagatc acccccaaaa
     1321 tcatcacggc aagaatcggc cctaacgaag caataccgac cattccaaaa ctgtcatttt
     1381 catttttctt ttgtttgatc atactagtca tccctccggc aagggccaag ataaatggaa
     1441 cagttactgg accagtagtc actccccccg aatcaaaagc aatcggcata aattcggaac
     1501 tggtgaaaaa tgaggcgatc aaaacgccta tgtagcctat agccatcaat tgataatagg
     1561 atagcttgaa tacgacccga agcagcgcaa aggctagaaa tactccagtg ccgacgctga
     1621 cgatcccaat cagcaacact ctgccaatct ctccgtcaga tatatcatat acttgttgtc
     1681 ccaaaacttg cacggaaggt tcagcaatcg tgatcacgat cccgatagca aatcccaaac
     1741 tgaccaaaac ggtcaaactt ttctttttga tcatatattt ccctactaga tctccgactt
     1801 ccatcataga ataatctgct ccaaacaaaa agagcgtcat tccgatcatc atgattgccg
     1861 caccaaccag aaatgaagcg aggtcttctc cttctaacgg tgcaaaaaca aacgttaaaa
     1921 tgacaatcag gacagtcatc ggcaatatag cagcaatcac ttctttaaaa ttttttagca
     1981 tgtatcctcc tcctttactc tttaagttta ccttaaaatg aaagagtttt cgagcacaaa
     2041 cttaaaacgt tatcatttaa tttttctcaa cataaaataa cacacgtccc aaaagtatct
     2101 ttttacttgg aaaaatgact ttgaaggaac gcgcttattg tgttttttta ttttacagag
     2161 ccgatcattc cttgaacgaa ttgcttttgc aaaacaaaaa atacgatggc agttggtaaa
     2221 gtagcgatga ctaatccagc catgatcatt ccatagtctg gtgtatagga agcacccata
     2281 gcagataaaa caagcggaac cgtccgttta tcaggagatt gcaaagctac taagggccat
     2341 aaataattgt tccagctact catgaatgtg atgattgcag ccgcggcata ggtattttta
     2401 gccgtcggca tatacactct aaaaaaaata cttagttctt ttagaccgtc taatcgagcg
     2461 gcttctacta gatcttttgg aaaagattta acattttgtc gaaagaagaa gatcaaaaaa
     2521 gcagtgctaa cagatggcag gatgacaacc aagaaactgt tgattcccaa aaccgttccg
     2581 ttcaattgtg aaaacattcg atataaaggg atcatcaatg ccgcaaaagg aaccatcatc
     2641 gatagcagta gaatattgaa aattaggtct tttcgtttac tttgataaat ctcaaatcca
     2701 tatcctgcca ttgacgaaat cagcaatgca aacacagtgg tcaccaaagc gatcatcgct
     2761 gaattccaaa gggaacgagt aaaattcagc tcattggaaa acaaattttg aaaattgatg
     2821 atcaagttac tgccaatctt tatttttccc gatgtgatat ccacgggtgt attcgtcatt
     2881 cccaaaacca tccagacaaa agggaaaatg gaaataaatg ataggatgct taatgctgta
     2941 tactttaata ctgacccaat tgtcgaacga agttctaatg cttgtttttt gacgttgggg
     3001 cgttcgattg tttctttttc taccatattt ttcatcgact ttctcctcct actttcgttt
     3061 ggattatcga caatatcaca ataaaggaaa caataacaaa ggaaactgct gccgcatatc
     3121 caaaattagg cgtgaattta taactcaaat tatagatgta ttgagatagg gtggtcgttg
     3181 catttgctgg tcccccgcca gtaatatttt gtacttcatc aaaaagctgc aatgtcccga
     3241 tcgtcgaagt aatggaagta aacaaaatga ttggttttaa atttgggatc gtcactttga
     3301 taaactgctg ccatttagaa gcaccgtcaa tagatgctgc ttcataaatc tccgaatcaa
     3361 tattttgcat gcctgaaagg aaaaaaatca tattgtatcc agtccaacgc caagtgatcg
     3421 aaagaatgat cagaactctc gcccaaattg gatgagtcaa ccagccaatc ggtgtgtcaa
     3481 tcataccaat gcccaacaac aagttgttta caaggccaga ttgagaaaat agacttttca
     3541 tgatcagaga ataactgacc atcgaagtga tacatggaag aaagatagcg gttcgaaaca
     3601 accctttata tttcaacttt ttatcattta aaaggttgga aataaaaagt gctagaaaca
     3661 acatgatcgg tacttgtaca agtaaataca ggaatgtatt aaaaaaagct tttttaaaag
     3721 ttgcatctgt caacatccgc tgataattac ctaacccgtt aaatgtcata ttgacagggg
     3781 gaccactttg aaaagacgtg atcaaagcca aaaccatcgg aataaaaacc atgattgata
     3841 tcaaaatgac tggcaagatt aaaaaagcat gcccgttttt gaataaatct ttctgcttca
     3901 ttcttttccc tccaattcta tcattgatta tctacatgaa aagacaagaa agacaatatc
     3961 tgtaaggcag acgtgatctt ttttgtcttt tcgtttgtct gatgaaaaag cgaagaaatt
     4021 cactttttta ccaccgatca attagccaat tgtgcttcta cttgtttttg tgtatctgct
     4081 agtaccttgt ctgtctcttc tccattgatg attcgatgaa gagcttcagc tacgactgct
     4141 tcaattgcat aggtctcatg tccgtaattc acttctggta tttcagatgt ccatgttgaa
     4201 aaatcgtcaa agatcttttg accactgtaa aattcagaag cttcttgata agcttcttgt
     4261 tcattcgcgc taagcatcgt agatactagt ccgatttctt tcgcaagagt gcccatcagt
     4321 tcttcattcg aagcaaacgt tgcttctagg aaatctttcg ctgaatcttc tccagctact
     4381 cctttgatca cataccagcc agcgccgcca aggttagaag cttgcgcttt ttgcagattt
     4441 tctggaagtg caggtattgg tgcaatcttc cattttccag attggttttc tgctccttgg
     4501 atcgttgaag aataccaagc gccagttggg ctagaagcta ctgctcctga ttgtatcgca
     4561 gttactccag cattccagtc agaggtttgt tcgactagat cttctttcaa aagagtggca
     4621 aacaattcaa gaccatactt taaagatgca ttgtcttgga tcgtgactgt ctccccatct
     4681 tcagatgtat accattctcc tgcctgttgc ataatcatcc ttactcgacc aaggtcagaa
     4741 ggattgactt cagcgatttt ctttcccgtc ttttctttga catctcgcgc tacttgaata
     4801 tactcgtccc acttcagatt ctccatatcc tcttctgtat aacctgcttc tgccatcaga
     4861 tccgtacgat agaagtttgc tgttacgccc gaatcgaaag gaacaccgta tattttatct
     4921 cctactttgt ttaccgcaaa tttatacggt gcaaaatgat cttcctttac aatagcagac
     4981 aaattagcga atgcatctgg ataagaagtc aaatagcctt gtatcctata atcttcaatc
     5041 aagacgatat tgggcaatcc ttctgtattc ccgctcgcta aagcggtgtt cagtttctga
     5101 acgatatcat cctgagacat cgtaacaact tctatctctg tttcttcgtt ctcataaact
     5161 ttttttgctt cattcactgc tttgatattg aatgtctcat cccacgccca gacagtgact
     5221 ttttcttctt tgtctgctgt actactagca cttccgcatc ctgttaagaa acctgatgct
     5281 gccgctaaac aaacgactcc agcaattaac tttctgatgt tcatttttat ccgctccttt
     5341 tccatccatt agttgtttac gtttgctaca ttcatcatac caaaatttga aaacggtttc
     5401 tttattattc agatacttca aaaatttctc ctaaaataat aaaaaaatac aaatcaacta
     5461 tgacaaaagg aataatc
//
LOCUS       DACDPS010000115         5468 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.115, whole genome shotgun sequence.
ACCESSION   DACDPS010000115 DACDPS010000000
VERSION     DACDPS010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.115"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(68..994)
                     /gene="glsA"
                     /locus_tag="IWJ07_002501"
     CDS             complement(68..994)
                     /gene="glsA"
                     /locus_tag="IWJ07_002501"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAP9334034.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(1017..2228)
                     /locus_tag="IWJ07_002502"
                     /pseudo
     CDS             complement(1017..2228)
                     /locus_tag="IWJ07_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            complement(2263..3876)
                     /locus_tag="IWJ07_002503"
     CDS             complement(2263..3876)
                     /locus_tag="IWJ07_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4368 domain-containing protein"
                     /protein_id="HAP9334035.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFDRPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIIEKIFSLAYQGYGSAKITKQLRAEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     GTNKTNKTPYSYYACSYYGQFGKGNCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASTLKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITSLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEDNERIQEIEIYYRFIGRVE"
     gene            complement(3988..4197)
                     /locus_tag="IWJ07_002504"
     CDS             complement(3988..4197)
                     /locus_tag="IWJ07_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HAP9334036.1"
                     /translation="MNCLERFYRKGVCVIDNAVKTIQKKNGKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
     gene            4288..4476
                     /locus_tag="IWJ07_002505"
     CDS             4288..4476
                     /locus_tag="IWJ07_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334037.1"
                     /translation="MAVREYHYLTVQVMAIKEDKSFTRTQIGFRSLLQGKGRGVFVST
                     ASCLSNVLNSFIQLSKND"
     gene            complement(5186..>5468)
                     /locus_tag="IWJ07_002506"
     CDS             complement(5186..>5468)
                     /locus_tag="IWJ07_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAP9334038.1"
                     /translation="WAWFVGENREPIRLTTKPEPYTLQKALHWLQRQVAPTIKMVQAL
                     DRENRTTILKDMIEQAELKDKHKHLLQLEKSTIEERIDTAVPQENDGIF"
BASE COUNT         1523 a         1191 c          919 g         1835 t
ORIGIN      
        1 aatttcagcc tcattgtagg tagctttgag ccgtttggac ttttacccca gcctctttca
       61 agaatggtta ttcaaagatg tcagcgtcta gttctttgac tacatctttc aacaattgga
      121 taccagctgc gctgttacca aatggatcta gtgccgggct gaagacacct atgccgtaac
      181 gatttggcac agctgccatc aacccgccgc ctacaccgct ttttgcaggt acaccaacat
      241 ggacggaaaa ctctcctgat tcatcataca aaccagcagt tgtcataatt gatttgacga
      301 ttgtagcact ttcttcagta atgatttgtt tgccattcca aggagcaatt cctttatttg
      361 ccagcacagc agctaatttt gctatgcctg tagcggtgac attgagggag cattgtctga
      421 aataagcatc gagtatctcc ggtacgtctc cttcgatcat ttgattacct ttcatatagt
      481 aagcaagaga gcgattgata tcacctgttc ttgattcaga atgatagatt tcttcattca
      541 atgtgatatt aggatcatca cagatctctt tcataaatga caagattcgc tcaaattttt
      601 catctgcatc tttaccggca atcaacgagg ttgttgcaat cgctcctgca ttaacaaaag
      661 gattcattgg atgtttgcga tgattgattt ccatattcat gatagaattg aaggcaaaac
      721 cggttggttc cgtgttgatt gtttgaaaga ctttttctat tccgttgtct ttgattgcta
      781 ataacaatac gggaactttt gacatgcttt cgatggcaaa gcgcacatcg gcgtctcctc
      841 cctcaatcag ttgttttgta tccaaatcat aaatcgccat acctaattgt ttgggatcca
      901 cttcagctaa tgctggaatg taatccgcca cttgtcctgt ttgataatag tggaaatttt
      961 ttccaatcaa ttgttctaat tcttttactt ccatttgaat aaccagcttt cattttttat
     1021 ttttctgcat gctggaattt atgacggcat gcgtagatcg taaatggaat aactaagacg
     1081 ataagataag aaacaatcag aatcgtttca taaatcccat gctgtccagc tgcgatacta
     1141 gcaggtggaa caaatgagat acaaaaagca aaaagtgaag tcaaaagacc taaacaagca
     1201 acgattactt ttacaacttt tccgcctgga acctgatagg ctctttcaag actgtcatct
     1261 ttaaaaacca gcttcaaata gcccgcgaaa aacataaagt acaacatcag gtagattaca
     1321 acagtcaatg acatcgctgc aagaaatgaa agattattcc ccccgccgct taatgtaaag
     1381 acggctgccc agattgttgc aacgatccca tttgcgatca tcaaatgagc aggaacaccg
     1441 tgtgtgtttt cttttgcaaa atattcagga agtaatcctt cttctgctgc tacgtgaagt
     1501 gcttttgaag gagaggttac ccaagaagca atttctccga tgacacctaa acaaacaaga
     1561 atcgcaagaa ttttcgcaat ccatgcaaga tttggatcaa tgtgtcttaa taggaattct
     1621 aacgattgaa tcacccctgt attaagagac aaaccatgtt gaggtactgt agtagcgacg
     1681 gtcaatccgc caaaagtatc taaaatgatt gccagaataa ccaatacaaa cattgctaat
     1741 ggataatcct ttttagggtt ttccatttca tttgcatgac tggcagatgt ttctacgccc
     1801 atatatgcta aaataaagga aacaaaaaca accagcgtag gcaatttcga aaaatcagga
     1861 atcaatgttt tcatagacag tatcgtttgt agtggagcgc ctgaagcgac ataatggatc
     1921 cctaagccaa acaagactaa tgctggcaac aagataccag ctacaaaacc gatccgagca
     1981 atcaatgaag tgtatttggt tccttttaac tgtgagagta aaatagccca gaaaataatc
     2041 aataggattg ccaatttcag atatttattg ttttgtatag caccaatacc cgttgcatag
     2101 gataatgcac cagttaagaa atacagcatg gtcaagtagc caactgttat ctcaaaccat
     2161 tggaaaaaga ttgcggcaaa accgaatcgt tttcccaatg tgcgactgac ccaagtatag
     2221 acaccgccgg tttcccttag ttaaaaatat tactcttgtt gattactcaa ctcttccaat
     2281 aaatcggtaa tatatctcaa tttcctgtat tctttcgtta tcctcattcg ttgttgcttc
     2341 atgtatgagg attttttcta tcatggcatt taataatggt gctgtcagtt cctttggtac
     2401 ggaatattcc ttgattaact caatccactt ttcagcacct atcatatcct gttccatttt
     2461 acttagttct tctcttaggc ttgttatctg ctgttccagt tctatctgtt ctttttggta
     2521 cttgctggac aacatcacaa aatttcgctc tgttatcttc tcacaggctc tatcttcata
     2581 cattttcgca aataaacggt caatctcatt ttgtctgttc tcggctttct tcaatgtact
     2641 tgcctttttc ttcttttccc gtattctctc tgcattgcca gctttctgta tcttgttcaa
     2701 taccttttct tcgtcctgct gtactgcctt agcccaatac tgcaagcgtt ccaatacggc
     2761 ttgatacagc acatcataac gaatgtagtg catagaacaa ttacctttgc caaactgccc
     2821 gtagtaactg caagcataat aactgtaagg cgttttattt gtcttattcg ttccaaatct
     2881 catagaccaa ccacaatccg cacacttgac aagccctgca aaaatcggcg ttgccttttc
     2941 ctttgtctgt ctgcgtcttg attttatctg ctcctgcaca cgatagaaca cttccttgtc
     3001 aataatcggc tcgtgagtgt tttctactcg aaaccattca ctttcgggct tacgcacttt
     3061 ctttttgctc ttgaacgata ccgtagactg catattgtga acactgtttc cgatataaac
     3121 ctcacttttc aatatcgcct tgacatgagc aatcgtccac tcataacgct tactttcggg
     3181 ttttccctca aagatatgtg caaaagtacc gtatcttgta aaatttagcc atgacgctgt
     3241 cggaactttt tctgctctta actgctttgt gatttttgca cttccgtaac cttgataggc
     3301 tagggaaaag attttttcaa taatccattt tgtttcatca tcaaccaaca gttttccttt
     3361 gatgtcaggg tgtttcttat atcctaacgg agcataagca ccgtaatgag ccccctccgc
     3421 aaactttgtc ttaaatgctg atttgacttt acggcttgta tctcttgcca cccattcatt
     3481 gatgatgttc ttaaatggtg caatctcact ttcgcctttt tcgctgtcta caccgtcatc
     3541 aatcgctatg tagcggacat tatgactagg gaaatacaat tctgtatatt gtcctgtcat
     3601 aatataattt ctgccaagac gggaaaggtc tttcgttaca acacagttga tttttcctgc
     3661 ctctatatcc tcaatcattc tttgaaaact cggtctgtca aaatttgttc ccgaccagcc
     3721 gtcatcaata tattcatcaa tgacattcaa atgatgttct tttgcatata aacgcaacat
     3781 acttctctgt gtggtaatgc tggaactttc gccctgtaat tcatcatctc ggcttaacct
     3841 aagataaagt gcagtattgt aaatctgttg tttcattgtc agttatcctc ctttataact
     3901 aacccgtgtt tacaatacct gcttgcaagt aaagtataac acgggtactt tttgtcattc
     3961 aatctatttc ttacaactgc accgatttta tgcgattgtc tgctttgctt tttccagcat
     4021 gacatcagtt agaagttctt ccattgtttt gcccttttcg gaaaaatgct cttttactac
     4081 aatctttgtc tttcctctgt tgccaatgcc acacttgcca tttttctttt gaattgtttt
     4141 tacagcatta tcaataacac ataccccctt tctgtaaaat ctttctaagc aattcaacgg
     4201 cttttgtcat agcccacagg agttccacct ctgcatagtt cttatgtagc cgtacccatt
     4261 gcgtgcgacg ctcttaatgc tcaagctgtg gctgtacggg agtatcatta tctgacagta
     4321 caggtcatgg cgataaaaga ggacaaatct tttacaagaa cacaaatagg ttttcgctcg
     4381 ctcttgcagg ggaaaggtcg tggcgtattt gtttcaacgg ctagctgtct gtcaaacgta
     4441 ttgaatagct ttattcagtt gtcaaagaac gattgaaaga aaaaaatcgt ctttcctata
     4501 tatcgcgttg gaaaagagga gaaacgtaac caaaatccaa aactttttcc attattagta
     4561 caaatggaac ggttcatttt ggcaatcaac ggttgatttt ctcctttttc actactatct
     4621 gtacagcaaa cgtccatttg ctaagtttaa atggaaaaat tttaacttct tttcatgcac
     4681 catataggga tagaaaatgt cattttgcta agttgatgat gaaaaaagaa tattttcctt
     4741 ttcacaccct aactacccaa accataccgt tactgccaac aatagaatga aatattttct
     4801 ttgcaccata tagggaacgg aaaccttaaa ttgttaagtt gagaacaaag tattttcatc
     4861 ttttcacatc aacactcaac tgctaatgcc tgtttgaata caaaatgatg atgtttttcc
     4921 tcttttcaca gaagactgtc cactaatcac gaatttccgt cattttttca aaactttttt
     4981 cttctttcac ttcatatagg gacagcaagc cccgatttaa tgacctattt tcaaaaaaag
     5041 tttagatttc cttttctcgc accatatctg tacgcaagta gccattttgt tgcagattta
     5101 ataaaaaatt tatttttctt ctttcacttc acatagggaa tgaaaacagc tctttgctga
     5161 ctaaatctga aatttttcaa ttacattaaa aaataccgtc attctcttgt ggaacagcgg
     5221 tatctatacg ttcttctatg gttgatttct ctaattgtaa taaatgttta tgcttgtctt
     5281 ttagttctgc ctgctcaatc atatctttca gtatcgttgt acgattttct ctgtctaatg
     5341 cctgtaccat ttttatggtt ggtgcgactt gtctttgtag ccaatgcaac gctttttgta
     5401 aagtgtaagg ctctggcttt gtggttaagc gtatcggctc tctgttttcc cctacaaacc
     5461 atgcccaa
//
LOCUS       DACDPS010000116         5417 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.116, whole genome shotgun sequence.
ACCESSION   DACDPS010000116 DACDPS010000000
VERSION     DACDPS010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.116"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            118..1068
                     /locus_tag="IWJ07_002507"
     CDS             118..1068
                     /locus_tag="IWJ07_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002821555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP9334039.1"
                     /translation="MLKTYSLTKDTLLVSSESNIKNSSVVYVYGDEREYIESSEHKYD
                     FKIGNILTFDDRVAYDTMIDKNDEKSLLVSFLFPEVHEGGHLDTLTTSVVFLVFKNKL
                     IIFTKQKLKFIPELLSNMVLRDVNHFVEEVLLKIMQKDFYMMLNDLRGVKEKIDDLDS
                     EISTSGSLKPTFGDLLTLQKYMIALSTTYKANHKALDFIKKNFTTIDDIDFSTKKIID
                     DLYDTSDTMDRIILSYSQYLDKLENMINNMISYQLNMIMKTLTEISIVLTIPAIIFGF
                     WGINVHVPFEKSSYGVLAVLIISAILSLICWLLMRRKTYL"
     gene            1586..2041
                     /locus_tag="IWJ07_002508"
     CDS             1586..2041
                     /locus_tag="IWJ07_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9334040.1"
                     /translation="MVKQIVLPIKDSSVLKMVQDTLLDSFRAGRRNYTVFQVGKATLL
                     RVSDVMTLKKSDVYNPDGSVKNATFIHDKKTGKANTLYLKPVQQDLLQYHDWLAQENI
                     NSEWLFPSTAHYDLHITEKQFHKVMAHVGDLLGINLEDKEKKLRVRKPF"
     gene            2352..2708
                     /locus_tag="IWJ07_002509"
                     /pseudo
     CDS             2352..2708
                     /locus_tag="IWJ07_002509"
                     /inference="COORDINATES: protein motif:HMM:NF013878.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2721..3131
                     /locus_tag="IWJ07_002510"
                     /pseudo
     CDS             2721..3131
                     /locus_tag="IWJ07_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812298.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
     gene            3162..3410
                     /locus_tag="IWJ07_002511"
     CDS             3162..3410
                     /locus_tag="IWJ07_002511"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase I"
                     /protein_id="HAP9334041.1"
                     /translation="MQSKVNQLYKATASQTLKAMQGLYEAKLLSYPRTDTPFITENEF
                     AYLKANFGKYSGFLGLDLEMLLSLIMIRSNNSTQILTN"
     gene            complement(3561..4540)
                     /locus_tag="IWJ07_002512"
                     /pseudo
     CDS             complement(3561..4540)
                     /locus_tag="IWJ07_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332307.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(4545..5312)
                     /locus_tag="IWJ07_002513"
     CDS             complement(4545..5312)
                     /locus_tag="IWJ07_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9334042.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
BASE COUNT         1808 a          890 c          950 g         1769 t
ORIGIN      
        1 taaaattttt ggcaggttat catacataag acaaaatgtt tttaaaactg ataacgtatt
       61 tattccacaa caattacgca tttatttctg agaaaaatat aaagcaggag gattacaatg
      121 ctgaaaactt attccttaac aaaagataca ttattagttt cgtcagaatc aaatatcaaa
      181 aattcttctg ttgtttatgt ttatggtgac gaacgagaat atatcgaatc ttctgaacat
      241 aaatatgact ttaaaatagg caatatacta acgtttgatg atcgagttgc atatgacacg
      301 atgatagata agaacgatga aaaaagtttg ctcgtaagtt ttttgtttcc cgaggtacat
      361 gaaggtgggc atctagatac cctgacgaca tctgttgttt tcttggtatt taaaaataag
      421 ttgataattt ttacaaaaca aaaattaaaa ttcattcccg aactcttatc caatatggtt
      481 ttacgtgatg tcaatcattt cgtggaggaa gtacttttaa agatcatgca aaaagatttc
      541 tatatgatgc ttaacgacct tcgtggagtc aaggaaaaaa ttgatgattt agactcagaa
      601 atatcaacgt ccggttcact caaaccaacg tttggtgatt tattgactct tcaaaaatac
      661 atgatagctt tgtcaacaac ttataaagct aaccataaag cgcttgattt cattaaaaaa
      721 aatttcacaa ctattgacga tattgatttt agtactaaaa aaataattga tgatttatac
      781 gatacaagtg atacaatgga tagaatcatt ttaagttaca gccaatattt ggacaaattg
      841 gaaaatatga taaacaatat gatttcgtat cagctaaaca tgattatgaa aaccctaacc
      901 gagatatcaa ttgttttgac tattccagca attatttttg gcttttgggg aatcaatgtc
      961 catgtcccgt ttgaaaagtc ttcttatggt gttttagccg tattgattat ctctgctata
     1021 ctaagtctaa tttgctggct tttgatgcga cgcaaaacat atttgtaaag tatttgtgat
     1081 accctaatat gatttaaagc agaatttctt tgttgacttt ttaaaaggct gattcctttc
     1141 aataatttaa tagcttcagc ggctgctcag aagtcaataa attggactat ttttattcag
     1201 ttacttaggc aacatttgag atacgattgc gattataaaa attgatatac caaccaatgg
     1261 ctgcttggaa gactgacata ttagcctcga tatcatcgaa taactaaata cagaaaaata
     1321 caataaatat tatcaaacta gggactatac tagaaaacat cagtacattc cttaactcag
     1381 ggtctataat tagaaaatcg cccctttgaa aactgattaa aacagccccc attatctagt
     1441 gactagataa tgggggctgt ttttttgtta agtgatataa taaaccataa aagaagttta
     1501 gaaatacggt cctggtcatc aataaataaa caatttaatt aattatttat ttaattattt
     1561 aatttcagaa aggtatacat gaatcatggt gaaacaaatt gtcctaccca tcaaagactc
     1621 cagcgtctta aagatggtac aagatacact tctcgatagt ttccgtgctg gtcgccgaaa
     1681 ttacaccgtt tttcaagttg gtaaggccac gctactacgt gtgagtgatg tcatgacatt
     1741 aaaaaaatca gatgtctata atccagacgg ctctgtcaaa aatgcaacct ttattcatga
     1801 taaaaagacc ggtaaagcaa acacgttata tttaaagcct gttcagcaag acttgttgca
     1861 atatcatgat tggctggccc aagaaaatat caattctgag tggttatttc cctcgacagc
     1921 ccattatgat cttcatatca ctgagaagca gtttcataag gtgatggcgc atgttggtga
     1981 tctcttagga atcaacttgg aagacaaaga gaaaaaattg cgagtcagaa aaccgttttg
     2041 agatatgaac agcatttgcc ttcacgcctt agcgatttta aataatagcc ttgaaagtga
     2101 ttatggaaaa gccctctacg ctcaatgcaa aattgacgta gaaactattt ttgatcgatt
     2161 gaaaggtgta tttggtatgt gaagaaccca tgtcagaggc aagaacgttg cacctactga
     2221 cattggaatg atgcttatga gcatgaatct aaccaaatta tcccttgagg ctagaagaaa
     2281 agcggaagct tttcaacaca aagcagccaa aaataaaaaa cgcgacgaag caatcacatt
     2341 tatgattatt catcgcgttt tttatattta gagctagtta tttgctagac ccactattta
     2401 cagatgaaac gtttatcacc tttggttttg ggcatttagt ggaattagca gaaccaggtc
     2461 attatgacga aaagtggcaa aattggaaac ttgaatcttt gccgattttt cctgatcgat
     2521 acgattttga agttgcaaaa gataagggaa agcagtttaa aattgttgca gaacttctca
     2581 aaaaggcaaa tacaattatt gttgcaacag atagcgacag agaaggtgaa aatatcgcct
     2641 ggtcgattat ccataatgca aatgcctttt caaaagataa aacatttaaa agactatgga
     2701 tcaatagctt agaaaaagat gtgatccgaa gcggttttca aaatttgcaa ccaggaatga
     2761 attactatcc cttttatcaa gaagcgcaaa cacgccaaat tgccgattgg ttgatcggca
     2821 tgaacgcaag ccctttgtat acgttaaatt tacaacagaa gggcgtacaa ggtacatttt
     2881 cactaggacg tgttcaaacg cccaccttat accttatttt tcagcgccag gaagccatag
     2941 agaattttaa aaaagaacct tttttcgagg tggaagctaa gtaaaacaag ggtcatttaa
     3001 gggcgttcta agccccacac agcgttttaa aacccaagag gagcttttta gcttttgttt
     3061 cttctaaaca agctaaaata ggcaatcaag aggggataat tgctgatgtt caaaccaaag
     3121 agaagaaaac gaatagtccg agttcgtttt ctttaagtag tttgcaatca aaagtcaatc
     3181 agctttataa agcgacagcg agccaaactt taaaagctat gcaaggactg tatgaagcaa
     3241 aattattgag ttatccaaga acagatacac catttattac agagaacgaa tttgcttatt
     3301 taaaagcgaa ttttggcaaa tatagcggtt ttttaggact tgatcttgaa atgttgttat
     3361 cattgataat gatacgttca aacaacagca cccaaatttt gacgaattag tgaaactcta
     3421 tgaaaaagac gtagtaaaac atgcaacatc ctattctaat cagctggtaa agcttaatta
     3481 ggttatataa gcttgaaaat aaagggtttg tccaatatgg acaagccttt ttcttatgtt
     3541 ttgccacaga tttgccacac ttattgtgta agagtatcaa ggataggtat aatttgatac
     3601 tttgtttttt tagtgatgtg caaataaatt tctgttacac gactacctct agaatgccct
     3661 acacggtctt gaattgcttc taggggtatt ccttactctg ctaagaaact aatatgcgta
     3721 tgacggaata tatgggtcgt aatatgttta ttaaaactaa gtttattatc aatatcaatt
     3781 cctgctcgtt ttggattacc ttttcgattt aattttccat tttcaggtga gttgaggaaa
     3841 ttgttaagga cactataatg aagaggtgtt ccattatagt cgggtttatg acgacttttg
     3901 gaagtaaaaa tccatccatt atctttataa tcaggattga atctcatatg ttggtgattt
     3961 aactcaattt cctctttaag aatttctata gcccttttag ataattggac atttcggtaa
     4021 ctttgtaagt tttttacatt accttcatag ctattttctg cagctcctcc gtgagtggac
     4081 tgactaatga tgatattttg cttttcaaaa tattgatagg caccaatatt ttggagtttc
     4141 gactctgtaa gtcaacaggg ccattagtta gaatacctat tgggatgcgg tttttatgta
     4201 gagccagcag tagttgatgt atctcaggaa tcatcgtaat ctgatcgagt tcatattcat
     4261 agtcagcttg gaagtctaga gcttctgtcc tagaaatact atcgatgcca aattcttcca
     4321 tagtcttctg acagcgaaaa attctaagct gctcaatact gatgagtttt ttcgtgtatt
     4381 taggaaaagc aacatcagac caatatctga aacgtttgta aatgttatct atttgttgga
     4441 tatcaatcgt tggaaaacat ttctcaacgc tacgtttaaa ggggagttga tggtcataaa
     4501 gagtgtcatc aaggtcaaaa acaatatttt tataagtcat aattctagtc cgcttttaaa
     4561 atagtagtgt actggctaaa ttcctctaag tgttgagggc taatctggtt atctgtaatg
     4621 atggtatcga gttgatctag attatagaag ttaaagaaat cgtatcgatc gaatttagta
     4681 ctgtctacta ataagaattt ttctacagca ttgtttaggg caagttgttg aatcacacct
     4741 tccttgtcac tatatgtagc aatagaatta tgggtaacag catttgcact aacaaaagct
     4801 ttggcaaatc taattgcttt taaatttgtc gaagccattg aacctacaaa agctccagtt
     4861 atttctctat attcaccgcc aagaagcaat aaatcaatgg tttcgctatc atttagaatc
     4921 aagaacacag ggagactatt tgtaatgaca cgaattttat aacctttacg acctttcaat
     4981 tctaatgcca gttgttcaag tgtagttcca ggtccaataa aaatagtatc gccatctttg
     5041 attaaagagc aggctttttt agcaatgtca attttttctt ttgtatttag acttttcttc
     5101 tcgatatgtg tcttttcaag tggcttttta gaggaaagca attgtgcacc accatgagta
     5161 cgtgttaaaa gtccatcagc ttctagagca tcaaggtctc tacgggctgt catatcggaa
     5221 atatctagtt catctattat ttcttttatg gttatggtcc catcaatctt taacatatct
     5281 aaaatttttt ctaatcgtcg ttttttgttc atatgaagac ccttctttca taaagtaatt
     5341 tttttccaaa gataattctc ttttaattgt atcataaaag acaatatttt caaggtaaaa
     5401 caaacaattt caaacaa
//
LOCUS       DACDPS010000117         5369 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.117, whole genome shotgun sequence.
ACCESSION   DACDPS010000117 DACDPS010000000
VERSION     DACDPS010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.117"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            19..936
                     /locus_tag="IWJ07_002514"
     CDS             19..936
                     /locus_tag="IWJ07_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9334043.1"
                     /translation="MPEISHEINIVTLTSDEKNKGLLDPYVIMKNPKDSESLAIDILT
                     FLTGISSRDGERFPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTD
                     YDFAHLLFSNGYVEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVIS
                     TFALDFIHTDRSIFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDD
                     LLDEKLKNNLGLKFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLY
                     GRVGVIQFHPVFEELLHAFDTRPPVRKEV"
     gene            939..3116
                     /locus_tag="IWJ07_002515"
     CDS             939..3116
                     /locus_tag="IWJ07_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP9334044.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            3113..4114
                     /locus_tag="IWJ07_002516"
     CDS             3113..4114
                     /locus_tag="IWJ07_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP9334045.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            4111..5043
                     /locus_tag="IWJ07_002517"
     CDS             4111..5043
                     /locus_tag="IWJ07_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9334046.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            5288..>5369
                     /locus_tag="IWJ07_002518"
     CDS             5288..>5369
                     /locus_tag="IWJ07_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="regulates the transcription of tet(M) by
                     attenuation; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAP9334047.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSI"
BASE COUNT         1702 a         1037 c         1131 g         1499 t
ORIGIN      
        1 ggcagatgga aagaaacctt gccagagatt tcccatgaaa tcaatatcgt cactctgact
       61 tctgatgaga aaaacaaagg cttacttgac ccttatgtga ttatgaaaaa tcccaaagat
      121 tctgaatcac tggctattga tattctgaca ttccttacgg ggatttcctc tcgtgatggg
      181 gaacgcttcc caatccttag aaaagccatt cgtgcagtaa ccaatagtga agtacgaggg
      241 ttgatgaaag tgattgagga attacgggtt gagaatacgc cactaagtac cagtatagcc
      301 gaccatatcg aaagttttac agactatgac tttgcacatt tattattcag taatggttat
      361 gtggagcagt ctatcagctt agaaaaacaa ctgaacatta tacaggttgc ggacttggta
      421 cttcccgaca aggaaacttc ctttgaggaa tataccacta tggagctttt atccgttgct
      481 atgctgattg tcattagtac ctttgcttta gactttatcc atacagaccg aagcattttc
      541 aagattgtag atttagacga agcatggagc tttttacagg tagcacaagg aaaaacacta
      601 tctatgaagc tggttcgggc tggtcgtgct atgaacgctg gggtatattt cgtgacccaa
      661 aatacagacg acctcttaga tgaaaaactg aaaaataacc tcggcttaaa atttgcattt
      721 cgttccactg accttaacga gattaaaaag accttagcct tttttggtgt agacccagag
      781 gacgaaaaca atcagaagcg attgcgtgat ttggaaaacg ggcaatgcct tatcagtgat
      841 ttatatggtc gtgtcggtgt gatacagttc caccctgtat ttgaagaact gctccatgcc
      901 tttgatacca gaccacctgt gcgaaaagag gtgtaaatgt gaaaccatca atagtaaaca
      961 gaataaaatc aaactggacg ctgaaacgtc taggtaaagt ggcaatgaca gtggctttca
     1021 cacttgtgat tgccattttt cttttagcca tgctgggaac ggtggttcaa gctgcgggct
     1081 tggtagatga tacggtcaat gtggcaaatg aatacagccg atacccactt gaaaactatc
     1141 aactggattt ttatgtggat aatagctggg gctggcttcc gtggaactgg tcggacggga
     1201 ttggaaaaca ggtcatgtat ggactatatg ccattaccaa ttttatttgg acaatcagtt
     1261 tgtatgtttc caatgcgaca ggttacttag tacaggaagc ctattcctta gacttcattt
     1321 ccgctacagc agattccatt ggtaagaata tgcagacctt agctggtgtg agtgcaaacg
     1381 gattttcaac agagggtttc tatgttggat tcctcttact cttgattttg gttcttgggg
     1441 tttatgttgc ctatacggga ctgataaaga gagaaaccac aaaggcaatt catgccatta
     1501 tgaattttgt gctggtgttt atcctatcgg cttcctttat tgcctacgct cccgactaca
     1561 ttaaaaaaat caatgacttt tcatcagaca tcagtaatgc cagtttatca cttggcacga
     1621 agattgtcat gccccattcc gatagtcaag gcaaggacag cgtggactta atcagagata
     1681 gcctgttttc catacaggtt cagcaaccgt ggctactgct tcaatacaac agttcagaca
     1741 ttgaaagtat cggtattgac cgtgtggaaa gcctgctctc caccagccca gattccaaca
     1801 atggcgaaga cagagaaaaa attgttgcgg aagaaattga agacagaagc aataccaatc
     1861 taaccattac aaagaccatt aaccgtttag gtacagtctt cttcctattt gtcttcaata
     1921 ttgggatttc catatttgta ttcctattaa caggaatcat gattttctcg caggtacttt
     1981 ttatcatcta tgctatgttt ctgcctgtga gctttatttt aagcatgatt ccatcatttg
     2041 atggtatgtc aaaacgagcc ataacaaagc tctttaatac cattttgaca cgagctggaa
     2101 tcacattgat tattacgaca gcatttagta tttcaaccat gctctatacc ttatcggctg
     2161 gttatccgtt ctttttgatt gcttttctac agattgtgac ctttgcagga atctacttca
     2221 agctgggcga tttaatgagt atgttttctc tacagagtaa cgattctcaa agtgtgggaa
     2281 gtcgtgtgat gagaaaacct cgtatgctta tgcacgctca catgcaccgt ctacagcgga
     2341 aacttggacg ttccatgact actctagggg ctgggtctgc cattgttaca ggtaaaaaag
     2401 gacagtcggg ttcggggagt tctgcaagga cacaagcaga tcactcccga ccagacggaa
     2461 aggaaaaatc aacacttgga aaacgtatcg gtcaaaccat cggtacagta gctgatacca
     2521 aagacagaat ggtagacact gctagtggtt tgaaagaaca ggttaaagat ttgccgacca
     2581 atgcaagata tgcagtatat caaggaaaat ccaaagtaaa agagaatgtc cgtgatttaa
     2641 ccagtagtat ttctcaaacc aaagcggaca gagccagtgg acgcaaggaa cagcaggaac
     2701 aaaggcgaaa aaccattgcg aagcgtcgct ctgaaatgga acaggtcaaa cagaaaaaac
     2761 agcctgcttc ttctgttcat gaaagaccga ctacaagaca agaacaatat catgatgaac
     2821 agacctcaaa acagtctaat attcagactt catataagga atctcaacaa gccaaacaag
     2881 agcgtccagc agttaagtcc gatttttcaa gtccaaaagt ggaacgccaa ggcaataccg
     2941 ttcaagaaaa aaccgttcaa aagccagcaa cttcaaccac tacagcagat agaacttcac
     3001 aacgtccaat cacaaaagaa cgtccgtcta ctgttcaaag agtaccacta caaaatacaa
     3061 gaagtagacc accaatcaaa accgccacca ttaagaaagt cggtaagaaa ccatgaagtt
     3121 gaaaacttta gtgattggtg gttctggatt attcttgatg gtcttctcac tgcttctgtt
     3181 tgttgccatt ttattttcag atgaacagga cagcggaatt tccaatattc attatggagg
     3241 tgtgaatgtt tccgcagaag tgctggctca taagcctatg gtagaaaaat atgccaaaga
     3301 atatggcgtt gaagaatatg tcaacatact tcttgcgatt atacaggtgg aatcgggcgg
     3361 tactgcggaa gatgttatgc agtcctcgga atccctcggt cttccaccta attcattgag
     3421 tacagaagaa tccattaagc aaggtgtgaa gtatttcagt gaattattag ccagtagcga
     3481 aaggctcagt gtagatttag aatcggttat ccagtcctac aattatggtg gtggtttctt
     3541 agggtatgtg gctaatcgtg gaaataaata tacctttgaa ctggctcaaa gtttctcaaa
     3601 agagtattca ggtggcgaaa aagtgtctta ccccaatccc atagccatac ctatcaatgg
     3661 gggctggcga tacaactatg gcaatatgtt ttatgtgcaa ctggtaacgc agtatcttgt
     3721 cacaacagag tttgatgatg atacggtaca agccatcatg gacgaagcac tgaaatatga
     3781 gggctggcga tacgtttacg gtggagcttc cccgactact tcttttgatt gtagcggact
     3841 gacacaatgg acgtatggaa aagctggaat taacttacca cgaaccgcac aacagcaata
     3901 tgatgtgacc cagcatatcc cactatcgga agcacaagct ggcgatttgg ttttctttca
     3961 ttctacctat aacgctggct cttatattac tcatgttggg atataccttg gcaataaccg
     4021 tatgtttcat gcaggcgacc caatcggtta tgccgactta acaagcccct actggcaaca
     4081 gcatttagtg ggagcaggac gaatcaaaca atgagaaagg aagatttaat gatgaaattt
     4141 agaaaaaatc agaataaaga aaaacagata ccaaaggaaa agaaacctcg tgtctataag
     4201 gtcaatcctc ataaaaaggt tgtgattgcc ttgtgggtac ttttagggct tagtttcagc
     4261 tttgcgatat tcaagcactt tacagctata gatactcata ctattcacga aacaactatc
     4321 atagaaaagg aatacgttga tactcatcat gtagaaaatt ttgtagagaa ctttgcgaaa
     4381 gtctactatt catgggagca atccgataag tccattgata atcgaatgga aagtctaaaa
     4441 ggctatctga cagatgaact tcaagctctc aatgttgata cagtacgcaa agatattcct
     4501 gtatcgtctt ctgtaagagg atttcagata tggacggtag agccaactgg cgacaatgag
     4561 tttaatgtaa cctacagtgt agaccagctc attacagagg gagaaaatac aaagaccgtc
     4621 cactctgctt atatagtgag tgtctatgta gatggttctg gaaatatggt actggttaag
     4681 aatccgacca ttaccaacat acctaagaaa tcaagttata aaccaaaagc cattgaaagt
     4741 gaggggacgg ttgattccat tacaaccaat gaaatcaatg agtttttaac gacgttcttc
     4801 aagctctatc ctacagcgac agccagtgaa ctttcctact atgtgaatga cgggatatta
     4861 aaaccaatcg gaaaagagta catctttcaa gaactggtaa atcctattca caatcgtaag
     4921 gataatcaag tcacggtatc gctgacagtg gagtatatcg accagcagac caaagcaacg
     4981 caggtatctc aatttgattt ggtacttgaa aagaacggga gtaattggaa gattatagaa
     5041 taacaaatat tggtacatta ttacagctat tttgtaatca cgtactctct ttgataaaaa
     5101 attggagatt cctttacaaa tatgctctta cgtgctatta tttaagtatc tatttaaaag
     5161 gagttaataa atatgcggca aggtattatt aaataaactg tcaatttgat agcgggaaca
     5221 aataattgga tgtccttttt taggagggct tagttttttg tacccagttt aagaatacct
     5281 ttatcatgtg attctaaagt atccggagaa tatctgtatg ctttgtatgc ctatggttat
     5341 gcataaaaat cccagtgata agagtattt
//
LOCUS       DACDPS010000118         5315 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.118, whole genome shotgun sequence.
ACCESSION   DACDPS010000118 DACDPS010000000
VERSION     DACDPS010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.118"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            36..197
                     /locus_tag="IWJ07_002519"
     CDS             36..197
                     /locus_tag="IWJ07_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002392227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334048.1"
                     /translation="MAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGVKDVLL
                     SLFNRIVGA"
     gene            210..713
                     /locus_tag="IWJ07_002520"
     CDS             210..713
                     /locus_tag="IWJ07_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP9334049.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            773..1162
                     /locus_tag="IWJ07_002521"
     CDS             773..1162
                     /locus_tag="IWJ07_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9334050.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            1149..2651
                     /locus_tag="IWJ07_002522"
                     /pseudo
     CDS             1149..2651
                     /locus_tag="IWJ07_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
     gene            3396..5222
                     /gene="ltrA"
                     /locus_tag="IWJ07_002523"
     CDS             3396..5222
                     /gene="ltrA"
                     /locus_tag="IWJ07_002523"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP9334051.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
BASE COUNT         1743 a          905 c         1201 g         1466 t
ORIGIN      
        1 aatggttttt aaccaatgcc caatccttag tcttactggc aattgtggtc attggtttgt
       61 ttttaggatt taagcgggag ttttccaaac tgattggctt tttggtgatt gctttgattg
      121 cggtcggatt ggtctttaat gcttctgggg tgaaggatgt cttactcagt ttgtttaacc
      181 gaattgtcgg tgcgtaaggg ggaagcacga tggaacagat gcgtgtttat attgcgaacc
      241 tcgggaaata taacgaaggt gaattggtgg gggcgtggtt taccccacct gtcgattttg
      301 atgaagtgaa agaacgaatt gggttaaatg acgaatacga ggaatatgca attcatgatt
      361 atgaacttcc ctttgagatt gatgagtata cgccaattga ggaaattaat cggttgtgtg
      421 gcttagcgga ggaacttgaa ggaacgccag ttggtgaagt tgcttctgag attcaacatg
      481 ctttcttcaa ttcttttgag gagatggtag agcatgtgga tgatattatc tattatccag
      541 attgcgatga tatgagtgat gtagcatatt tcttgattgt agaagctggt gattttggag
      601 aagtaccaga acacttgaag acgtatattg attttgatgc gtgtggtaga gacttggaga
      661 ttggaggcaa ttatttggta acgagtcgag gtgtttttga gtatccatat taacaaaatt
      721 attgaagggt agttgatttc taaactatcc ttctttttaa aggaatgatt tgatgaagaa
      781 aataaaaagc tacaccagta tttgggcagt tgaaaaggtc atttatgcaa ttaatgactt
      841 tcaactgcct tttcctgtga cttttaacca gatggcttgg tttgttttat ccttgctgtt
      901 tgtcatcgta tttgcccatg tgcctccact atctatgata gagggggcct ttttaaaata
      961 cttaggtata ccggtggcag ttacttggtt catgtcgcaa aaaacgtttg acggtaagaa
     1021 accattggga tttttacgat cttttattag ctatcactta cgatttaagg tcactttcgc
     1081 aggaaagaaa gtcaaagaac agaagaaacg atgggacgaa cccattactc tggtaaggag
     1141 tgtgaactat gtacccgatt aagtatattg aaaataatct tgtctttaat caagagggag
     1201 aatgttttgc ctactatgag ttaatacctt acaattactc ctttttgtca cctgaacaga
     1261 aatatcaagt ccacgataac tttcgtcaat tgattgcgca aaatcgtgaa ggcaagattc
     1321 atgccttaca aattgccaca gaaagtagta ttcgtgccac gcaagaacga tcaaaaaagg
     1381 aaattaccgg gcgattaaca gaagtagcga aacaacgaat tgatttacag acagaggcat
     1441 tagtatccat gattggcgat agtcagattg attatcgctt ttttattggc ttcaaactga
     1501 ttgcgacaga tgaagaggtc aatctaaaga atctcaagaa atctttcttc tccggctttc
     1561 aagaatttgt ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg
     1621 aagaaattcg ccgatatacc aagcttgaaa aactaatgga aagtaaattg gctcgaagat
     1681 ttaaagtcag acgagtcaca ccgagtgatc tgatgtattt gattgaacat atctatggcg
     1741 aaaagggtac accatttgaa gaatatgaat ttcagctacc aaagaaaaag ctgaagtcgg
     1801 aaactttggt gaaacgttac gacttattac gtcccagtcg ttgtttgatt gaagaaaagc
     1861 cccggtactt atgcatggaa catgagaatc atgaatcata tgtagcgtac ttaacaatta
     1921 atacaattgt gggggaaatg gagtttccct cctcagaact tttctattac cagcaacaac
     1981 aatttacatt cccaattgac actagtatga atgtggagat tgtgacgaac aaaaaggcac
     2041 ttgctaccgt tcgcaataag aaaaaggaat taaaagactt ggacaaccat gcctatcaat
     2101 ctgacaatga aacaaattcc aatgtcttag atgcattgga ttccgtagat gaattggaaa
     2161 ctaccttgga tcagtccaaa gaatccatgt acaaactgag ctatgtggtt cgagtaagtg
     2221 ccgaatcagt agatgaacta aagcgccggt gtgatgaagt gctagatttt tatgatgata
     2281 ccaatgtaaa actagtccgg ccttttggcg atatgatggg gctacatgaa gaattcttac
     2341 cttctagtaa gcgatacatg aatgattata tccagtatgt tacgtctgat tttctcgccg
     2401 gacttggttt tggcgccact cagatgttag gggagttgga aggcatttac tttggttata
     2461 acgtagatac ggggcgcaat gtttacctga aaccggcatt ggcctcacaa ggggtcaagg
     2521 gttcagttac caatgcgcta gcagcggcat tcctcggttc actcggtggc ggaaagtcct
     2581 ttagtaacaa tctcttagtt tattacgccg tcctttttgg tggacaagct gtgattgtcg
     2641 atccaaaagg gggtgcgaaa cgttcctact tgaaaagagt aggcacagca cttcattaaa
     2701 tcaatattta atgtgtgttg gagaactaaa cacacgaata gtcgaatgac ggggtaacgc
     2761 cctgaaaggc tactttaata ctctgactgg cagaaacttg agtaaacaag gctctgtcag
     2821 aagctcggtg aagtcggcag acagacaccg taacagataa tgctgacgaa agattcccag
     2881 aggtgggcgt tgtgtcctat atgccggaag tctataaaat atctatggtt agaatgtaga
     2941 tgagaggtct actgacgaat ccttgaatgt acaggtctat atcggggctg gatagaaatg
     3001 tccagtgaca caaacagtgt cgctactgtg ggtgagtaag actccgcatt tatgaaaatc
     3061 catataacgt taaaggcgtt atcaaggtag caggcttata gaggaaacct aaggatatat
     3121 gtacagatag tgtgtttgga acgttgaaag ctgacaacat ggagatttta ctgtctgtga
     3181 agtgttcaca tcagaaagtt attgtgagat tagcaatgat ataaggtgtg ttaatgtcag
     3241 tgagagtggt ggcacagtac ctatgaaacc gtgataacaa gcggtggagg gatagccact
     3301 agtcatatga agaatagaat tactaagaaa aatggtatgg gttcgagtaa gactaagaga
     3361 tgtacttgct cctgcaaaca aggagggtac agaccatgct aaagaaaaac aagctccgct
     3421 ataacgagta ttatgatatg caacatattt atgacgagtt atacgcacag agcaagaacg
     3481 gtaataactt ttataagtta cttgaaatta ttggttcgga acaaaatatt tgtttagctt
     3541 accgtaatct gaaatcaaac agtggcagta aaacggctgg aacggatgga atgacgatag
     3601 acgatataaa acaactcagc aatgctgaga ttgttgctac cgttcgggaa agccttagca
     3661 actaccgccc caaatcagtc agacgtgttt tcattccaaa agctgggagc gataaaatgc
     3721 gtccattggg gattccgtgt atctgggaca ggctggtaca acaatgtatc ctgcaagtcc
     3781 tagagccgat atgcgaacca aagttccata atcattccta tggattccgt gcgaatcgaa
     3841 gtgcccacca tgcagtcagc agagtaacga cactgataaa tttatcaaaa tatcattact
     3901 gtgttgacgt agacattaaa ggatttttcg ataatgtcaa tcatggaaaa ctattaaaac
     3961 aaatctggac acttggtatt cgggataaaa gattgatttg cattatcagc aaaatgctga
     4021 aagccgaaat tgacggcgag ggtgtcccag aaaaaggaac gccacaaggc ggtctgttaa
     4081 gtccactgct ctccctcata gtccttaatg aattagactg gtgggtaagc agtcagtggg
     4141 aaacattcca gccgaagaac cgcagtaaaa atgggtggct tcaatacgca aagaaatata
     4201 ccaaactgaa aagcggtttt attgtacgtt acgctgatga ttttaaaatc atgtgttcaa
     4261 cctatggaga agcacaaaga ttttaccaca gtaccgtaga ttttcttaat aaaaggctga
     4321 aattagaaat cagtccagag aaatcaaagg tagtaaatct gaagaaaaat tcatcagatt
     4381 ttctggggtt caaaatcaaa gtcattccaa aaggaagaac gaaacacggt tatgttgcca
     4441 aaacagatat gaaccaaaaa gcattaaaga aagcgaaaac aaatctaaag ttaaaggtaa
     4501 aagatatagc cagacatacg actggcttca atatcagcag atataatctg acagttatcg
     4561 gtatgcagaa ttactactgc atagcaacta acgtatataa caacctgaca gaagtaagtt
     4621 acgctctctt accgaccata cgaatccgtt taaggaacat tgcaaaatca gttccatttg
     4681 aaagtacaag ttcagaattt cagagcagga caaaaggaat cagaccaaaa acaaaaatag
     4741 tgatgattgc ggataatccg ctattgccga ttcaaggagt acaacacaaa aatcctatga
     4801 atttctcgca agatatttgt aattttacaa agcaagggag aaacaaggtg catgaggacg
     4861 ttgtagttgt aacaaaagaa gaaatccgag cgttgttgga aaatgaaaac cctgccgata
     4921 gcgtagagtt taacgacaat cgtatttcgg catatatcgc acaacaaggc aactgctatg
     4981 taatgaatcg tcgaggaact ccgtccactc tgatatgtat tcacaaatca gacaaaggag
     5041 ataaccttga cagatattct aatctggcat ttgtggaaat tcccattgca aaggcaatcc
     5101 taacagaatc agcagacgaa gcaaaatcac tgttaaaagg ttatgttctt aatagtcagc
     5161 agaagaaaaa actgaacaga atacggacga attacggata ccagacaata actgggaaat
     5221 aacttataaa tctatttgga tatgatggaa cgccgtatga agcgaaagtt tcatgtacgg
     5281 tgtgaagcgg gggaaaactt ggagataaca tcaaa
//
LOCUS       DACDPS010000119         5112 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.119, whole genome shotgun sequence.
ACCESSION   DACDPS010000119 DACDPS010000000
VERSION     DACDPS010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.119"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(81..1889)
                     /gene="pepF"
                     /locus_tag="IWJ07_002524"
     CDS             complement(81..1889)
                     /gene="pepF"
                     /locus_tag="IWJ07_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAP9334052.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2065..3063)
                     /locus_tag="IWJ07_002525"
     CDS             complement(2065..3063)
                     /locus_tag="IWJ07_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAP9334053.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3289..3942)
                     /locus_tag="IWJ07_002526"
     CDS             complement(3289..3942)
                     /locus_tag="IWJ07_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAP9334054.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4274..4672)
                     /gene="spxA"
                     /locus_tag="IWJ07_002527"
     CDS             complement(4274..4672)
                     /gene="spxA"
                     /locus_tag="IWJ07_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAP9334055.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1523 a         1045 c          773 g         1771 t
ORIGIN      
        1 aaaaaaatac gtcgactccc ttacaagaga gccgacgtat ttttttcgtt tctattcgag
       61 aaaatcaaaa atagaaagcc ttataaacga tcgatcaatt cttctaattc atttagacgt
      121 tgttcaaaca tcgacattgc atcttcaata tatgcagcct gtgtcatatc aactcctgct
      181 tttttcatta cttccacagg ataatcgctg ttacctgctt ttaggtaagc caagtagttc
      241 tctagtgcct ctggttcctg gtttagtatt ttcttcgcca gtgctgaagc agcagaaaag
      301 ccagtcgagt attggaaaac atagtaatta taataaaaat gcggtatccg tgaccattcg
      361 aatttaattt ctgggtcttc ttcgactgct ggaccataat attttgcatt caatttacca
      421 taactatcac ttaggtattc actagtcaat ggcacacctt tttcatcttc cgtatgcatg
      481 aaatgctcga attcagcgaa ttgtgtctgg cggaaaactg ttcctttaaa cccatccaag
      541 taatggttga gtacataagc ccgtacacga gggtcttttt ctgtttccaa taaatactcc
      601 gttaggatat tttcattcgt tgtcgaagca atttctgcta aaaagatgga gtagtcgccg
      661 tacacataag gctggttcga acgagtgaaa taactatgaa cactatgtcc catttcgtgg
      721 acaagcgtaa atagctgatc cagcgtatca tgccaattca ataaaatata tggatttgtg
      781 tcatagcttc ccgaagaata agcaccgctt cgtttccctt tattttcgac aacatcgatc
      841 caacgttcag agaatgcttt ttctacgatg gccatgtatt cttcacccat tggtttcagt
      901 gcttctaaag ctttttcttt tgcttcttcg tacgtaaagg taattggagc ttcccctaag
      961 accggtgtat aaagatcata catgtgcagt ttttccactt ctaataaacg tttccgtaat
     1021 tccatgtatc gatgtaacaa aggcaaatgc ttgtttacca cgtctaccaa agtatcgtat
     1081 acactttcag gaatatgatt attgctcaaa gacgcttctc tggcagagct gtaattacgg
     1141 actttcgctt taaaattatg tccttttata tgtgtgccta aagtagaagc aaatgtattt
     1201 ctaaattgtt cgtaaacact gtacaacccc ttaaatgctg cttcgcgcac ccttcgatcc
     1261 gtgctttcta gcaactgacc atacacgcca tgagataatt ggactatttc accattttct
     1321 ccttcaatcg ttggaaaaac tagatctgca ttattcaaaa cagcaaatgt atcacttgaa
     1381 gcatcaaaga tttcacctgc tccagcaaga agagattcct gctcagcaga taagacatgg
     1441 gctcgattat ctactatttg ctggatataa tggcgataga cttccaattt tggttcttct
     1501 ttgaaatatt gccaaatctg gtcatctgac aattgcaata tttctggttc aaaccaggaa
     1561 acggcttcac taactttcga aaacagactg cttgctcttg cataaagcgc ttggtaatct
     1621 gtatttccag tgtcttgatc gtttttaagg tgcgcatata cataaatgac ttcagtttgg
     1681 cgataaaccc tcaatacgaa ttcaatcgca tttaaaaatt gagaagcgcc ttgatcaagt
     1741 gttcctttgt gtttttcaga ttgttttaac tcttctgata attccaaata tttctcatca
     1801 aactcttggt cgcttgaaaa gatcttggtc aagtcccaag ttaaattttc aggcaattct
     1861 tctcgttttg gcaactgctt tacttccatg aaatccactc cttttcttcc cttagctcta
     1921 tctatcaaaa tagatcatag tctaacatcc acatgtcaca gcatctaact tttctactca
     1981 ttcatgtgtc tgcttccatc aattttagca taactaaatc ttgttttaca tagaaaaccg
     2041 attcctagcc tatttttata cttactattt tttcaaagaa gacaagtgta tgcattcgtt
     2101 aaaaaactgc tgatagacca tctcttcatc aatcatagga aaaagccatt cccttttatg
     2161 ttcttttatt tggcaaagcc atgagttgaa atcaggaact ttctggtttt ccaaggcttc
     2221 ttcgtacaac attcgataaa gaaaaacctg ttcttgtaaa taaaagaaaa accgactttt
     2281 ttgatagatc cacgatgata gatgcaaaag atgtttatga cgcaagtagc attgttcttg
     2341 tatggatact gtattctttt gcttgtgaat aagttggcgt gtcagccatt tcttcccatc
     2401 ttcactcact ttttctattt ttttcatttg gttcatttta atttcgttac tagtgaatat
     2461 ttctttaagg tccctactag aaaaagtcca gcagctactg caaaaggaaa tatgccctga
     2521 tacagtctct gtaatatgat gatacaaata aagcttttgt ttcttgagat cagctttcca
     2581 gcaatgcact cctctttttc tattatagta gcagcaactt ttttctatca atgagaattg
     2641 cgattgtcca agaaaagaat taccaagtat ccaccatact tggtaactgt tattcaaata
     2701 attttgggtt ctttcaataa atctctggtg agacagtcta gaacattgga tttctattgc
     2761 tagattcgcc aacaaaatat ccggcctttg ccgcaacctc ggcaaatagg cttccaaaaa
     2821 tactggttct actgactgtc ccagccaatc catgaagcat tcctttaact gtaagtgctc
     2881 ttttgattct ctttcactaa agaccgaaca gttatctgat ttttgatggg caaaatgagc
     2941 acattttttc tttccctttt tcaaaatcac tctttttcga cagccacgac aataatattt
     3001 ctttcctttt tctgcatgtt tagcaaaaca gcacttatta tattcattca ttgcaagcag
     3061 catttttctc cctctgaact tatttacgtc taagaattta tttacacgaa aaaagagggc
     3121 gtgaaaaagc tattctgcat caagtaataa gaacagctaa acaaaaatag ttcccaatat
     3181 ttttcgtttg gttgggcttg ttaccgcaga tgcaagcttt tgaacacatt tatacgaaaa
     3241 aaagactgcg acacaatttt tgtcacagtc tcttactata atgctgattt atacaaaata
     3301 aaatttcgtt cgctcgatag cattacgcgc catcagacat tgtccatatt cattcaatgt
     3361 ttctggtgta cgatcgctct tttcagcaaa ctccaaaatt cgagcaatct gattctctac
     3421 atccgcttgg ttgagatttt ccatccagaa gtgaacgact aggtaatagc gctcttcgaa
     3481 taaatacaaa tcagtccaag cagactctag ttcgacatct gttgcaagtt ggatcacttg
     3541 ttcaaaatct tcaaaagcaa aagttgcttg tctttcctgt tcttctaggg cgcttccaga
     3601 taattcttcc tccggatcgc tgccaataat ctgctgacga agaaagtcac tcacatcttc
     3661 tgaatcaaca gtagaatctt ctgattgaga aatttcttca tccggaagat ttttactgat
     3721 aaacagttct aatccgtctc cttttggcaa aacttggaaa gtgacagctt cgcttcctcg
     3781 gaactcctct tcgatatcaa cttcttccaa aatactataa aagaaatttt cgatttcttt
     3841 gtgattgcca agtaaatcca aaaatgaaac gccccgttcc tctaaatctt catgaccaat
     3901 tagtacacgg atcgtatttt cattaatatg ttccatttcc attttgctac acctcacttt
     3961 gccacaatgt ttaatataga ttcattgtag cgaaatcagc ttaaatgtaa agaaaaaaaa
     4021 tctcgctgtt aagcgaattt aataaaacca gcggattttt caacctttca agctatctta
     4081 tttaactata acatttttgg cagacgaata ctatatagcc gacttttcag ctaattttcc
     4141 ctctctattt ttcgtgcaat catcatcttc tactaccttt ttatacatct aatcaagaat
     4201 actttttgta aaaacaaaaa aacttggaaa aactatcttt gtaatagtct tttccaagtt
     4261 tcgtattatc cttctataat ccagccatca attgtgcttg acgtaattct aattgtcgta
     4321 catcgcgcgg taggaaccga cgaatttcat cttcattaaa accaacttgc agacgtttat
     4381 catcaatcat gattggacgg cgaagcaatc caggattgtt ttgcacaagt tccaataatt
     4441 cttgtaacgg cagatcatct aaatccatgt tcagtttttg gaatactttg gaacgtgtcg
     4501 agatgatttc ttctgttccg tcttctgtca tttgtaaaat cgcttttaat tcagaactgt
     4561 tcaatggttc tgaaaaaata ttacgttcaa caaatggaat ctgatgttct tgcaaccacg
     4621 cacgagcctt tctgcaggaa gtacaacttg gggaagtata taatgttaac aacgtgcacc
     4681 actccttttc gtgataagaa tcactttttt gtaacagaag atcataataa tctcccatct
     4741 gtttctatta tacataatca acttttaaat agctaccttt ttttcataat ttttttaaaa
     4801 aaatgaaagt tatctaaggt taatatacag taaaaccagc attctcgctt tttttatcat
     4861 ttaaaaatta gagttttcaa atctaattca aattttctaa acatatgaaa cagcgtataa
     4921 aactgtacta ttattttatc tcaaaagcta aatgaaaccg atttcttaag attttttatg
     4981 ttttttattt ctttaatagt aatgttttct gtcattttaa ataatactgt tctttattat
     5041 aacgcttatt ttctctgtta tataagcgta gtattttaaa gcaatgatta ctaaataaaa
     5101 tttactataa ac
//
LOCUS       DACDPS010000120         5104 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.120, whole genome shotgun sequence.
ACCESSION   DACDPS010000120 DACDPS010000000
VERSION     DACDPS010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.120"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..186)
                     /locus_tag="IWJ07_002528"
     CDS             complement(<1..186)
                     /locus_tag="IWJ07_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAP9334056.1"
                     /translation="MFYHLRKENSNENLDAIALTLLIVGGLNWLLVGLFELDLVAMIA
                     GGSTTIFAKIIYIVVGIC"
     gene            379..1179
                     /locus_tag="IWJ07_002529"
     CDS             379..1179
                     /locus_tag="IWJ07_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9334057.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            1198..3066
                     /locus_tag="IWJ07_002530"
     CDS             1198..3066
                     /locus_tag="IWJ07_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9334058.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELCDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            3059..3550
                     /locus_tag="IWJ07_002531"
     CDS             3059..3550
                     /locus_tag="IWJ07_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAP9334059.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            3538..4092
                     /gene="srlA"
                     /locus_tag="IWJ07_002532"
     CDS             3538..4092
                     /gene="srlA"
                     /locus_tag="IWJ07_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAP9334060.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            4110..>5104
                     /locus_tag="IWJ07_002533"
     CDS             4110..>5104
                     /locus_tag="IWJ07_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAP9334061.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     EKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         1788 a          812 c         1054 g         1450 t
ORIGIN      
        1 acaaatacct acgacgatat aaatgatctt cgcaaaaata gttgttgaac caccagcaat
       61 catagctact aaatcaagtt cgaacaagcc taccaacagc cagttcaacc ctcccacaat
      121 caataaagtt aatgcaatag catctaagtt ttcattagaa ttctcctttc ttaaatgata
      181 aaacaattat atatcaaaaa gagaaacatt gcttatataa cgctcaggaa ttttagcaat
      241 ctatgcaaaa agatgaaaat tttttttgct cttccattag tacacatttt ttagctgaag
      301 ctgtaaattt catgtcttaa ttttttttag tgagctttta tactggattt gtaagaaaaa
      361 agaaggagag gatcacatat gtcggattgg ttggatattg caggaaaagt agtaattgta
      421 actggaggtt cctcaggaat tggaagaagt atcgtcgaaa atttgttgaa gcagaatgca
      481 caagtagcga attttgatgt aactgaatgc cgtatacagc atgaaaacct actatcttta
      541 aaagtcgatg tgtcttctaa aacggacata gaagaaggga tatataaagt aatgaaacat
      601 ttcaagacga tcgatggtct agtcaataat gcaggaataa atattccttc tcttctaata
      661 gatagaaatc atccaaaaag caagtatgaa ttatcagaac aagtttttga taagatgatc
      721 gctgtcaatc aaaaaagtgt atatttgatg agccaagcag ttggaagaat cttggtgcaa
      781 aagggatcag gagtgatcgt gaatctttct tctgaatcag gattggaagg atcagaagga
      841 cagagctgtt atgctgctac aaaagcagcg atgaacagtt ttactcgttc ttgggcaaaa
      901 gagttaggaa agaggaatgt tcgtgtggta ggtgttgcac cagggatact ggaagaaacc
      961 ggtttgagaa cacaagaata tgaagaagct ctttcttata cgagaggtat aagcgtcgaa
     1021 cagctgagaa acggttacag tcgaacaagt acgatccctc taggaaggag tggtaagctg
     1081 caagaagtgg cggatctagt ctgctattat ctttcagaaa gatcaagtta tatcactgga
     1141 gtcacgacta atatttcagg aggtaaaaca agaggataaa ggataggggg gacgcacgtg
     1201 acgctagtaa acagatggta ccaattattg aaattactag tcactcacaa gaaagtttca
     1261 atccaggaag tgcaaaaaga aatcaaaacc agtaatcaaa ccataaaaaa agatattggg
     1321 gagttgaatg aagaaattgc cggtattgca aaaatcatcc aaaaaaacaa acattttgaa
     1381 cttgaaattt atgattttga tttttttgat gagatcatgc aaggaaaatt gaaaacagcc
     1441 agtgatttca attcggcatc caaaagaatc gcttatttac taaataggct gatcgaaaca
     1501 gaggatttct tacgtatgga tgatttgtct gaagaagtcg gtgtcagcag aggaacagtg
     1561 gtcaaggatc taaagacatt aaaagagatg atcaatgatt ttgatgtaaa gattacaggc
     1621 acaccaaata aaggactaag aataatagga gatgagctag aattacgact acttttattt
     1681 tattttgttt ctccctactt ttcagagacg aaaacagaac agtttctaga agaaaaacaa
     1741 gcaatcaaac attttcagaa gaagacgaat gctagtaaac aagaaatcac ccttttaacg
     1801 aaagttatcg gtatttcttt gaatagaatc tctagtaact atctgttgaa gaagcccatc
     1861 aatcgttaca gtgggtgttt tgaatatatc caagaattca atgaactgat caatcagttg
     1921 gaagaaatct atgaactaac tttgagccaa tatgaaaaag attttatcag ttttcctttg
     1981 aacatcatca acacaagtat aacgatgata aggaaagcta actctgattt gcgcttatat
     2041 ttcgacccca tgatgatgcg tattcgacga ttagatctca ttgatctgga tgagtccttc
     2101 ttatatcaag agatgaaaca gcacctctct ttattgataa atcgggtaat ttttcgttat
     2161 cgattgactg atttatttta tggtgagata gaaaaaaagt atcctttttc ttaccaaatc
     2221 gctagcgaat gcatacaagc attagaagct atcttgcaac ataagatctc taaagtagag
     2281 atcagctacc taacgttgta tttggagtta gcatttcgat atcaaaaaga gcatttttcc
     2341 tctaaaaaag tagccattgt tcatacgaca ggaaaaggaa cagcattaat gatccaaaga
     2401 caaatcaaaa ggatattggg gagggatata caaataacac atgtttcaga agaacaatat
     2461 gaaaaagtag atctaaacaa atatttcgct gtttttacaa cgattccgtt aaaaaatgta
     2521 aatgaagaga caccggtgat ccatttgact aatctttttg atgactcttg gttaagggca
     2581 caatggcaaa aagcaaaaaa aaagcgatta gccgggtttc attctactgt ttttttctat
     2641 caaatatgga aagaaaaaaa gacgtatcaa gaatatttaa acaaattagt cgctcctttg
     2701 atcagaggct attatgtgga tgaagaattt gaaaaaagga tcttgaaaag agaatatcag
     2761 caaagcacat ttgttaacaa agaatttgct tttcctcaca caaccaacca gctatctcaa
     2821 cacattgttt tatcagtagg gctaataact gatcaaggtt tagcagaaac aggaggaaat
     2881 ggagttattt ttttatttgc catacctgaa gaaatgacgg ctgaagttga gaatgaatta
     2941 tttgagctat gcgatatcct atttgaactc ataaaaatgg gaatagatga gtttattgaa
     3001 ttagcaccga cacaagaatc atttttaaaa ctaatcaaaa gtaaaggaga gagacttcat
     3061 gaatgatatg tatctattca tagttttcgc agcttcagct tatgtgctgc aagcagttat
     3121 cagttggaag caactaaagc atttcaatca aacatatagc gaactgcgaa gaaaaggaaa
     3181 agtagcgatt ggtaaacgac ctgggaaaat ccgttcaggc acgattgtta tgctagctat
     3241 agacgatcat gatcaagttt tagaagtcag aaaaatgcaa ggggttacat ctttggcaaa
     3301 atttcattca ttacctgtct atgcgggtga agatatccat tacatcgatc gttaccatcc
     3361 ctcagttcga gcggaagatc gtttgacaca agcagcttta gaaaatgcca gagaagtata
     3421 cttacgaata gaggcaggaa atgaaaaaag tgaacaacct tcattgcctt ttattgctat
     3481 gctcaataat tttcggatat ggaaaagtac gatcaaagaa aaaaggagtg acaaacgatg
     3541 gatatcataa caaaatttgc tgagggattt atgaggctgt tccaaacagg tgcagaaaca
     3601 ttcataagct ggatgacagg aatcgtacca gttgtcttga tgctgatggt tgcaatgaat
     3661 acgctgattg cttttttagg tgaagaccga gtaacgaaag tagccaaatt atcagcgaaa
     3721 aatccgctta ctcgttacct tgtgttaccg ttcttatctg cttttatgtt gggaaatcct
     3781 atgtcattta caatggctcg tttccttcca gaatactaca aaccaagcta ttatgctgct
     3841 caagctcaat tttgtcatac gagcaatggt gtattccccc atattaatcc tggagaattg
     3901 tttgtatgga tggggattgc tcaagggatc gaaaccttag ggctaaattc gatggagctg
     3961 gctatccgat acttgcttgt gggactgctg atgaacttta ttggtggttg gataactgat
     4021 tttactactg gatttgtatg cagacagcaa ggaatcgttc ttagcaaaaa agtagagctt
     4081 tcagttgatt agagaaagga gagttttaaa tgtatggtga tattaagatc gtaagaggaa
     4141 acggcgggta tggaggtcct ttgattatta ggccgacaaa agataaacac aagttcatct
     4201 atgtcacagg tggaggtgag aggccaatga tcgtagataa aatagaagaa ctgacaggta
     4261 tggagtctat caatgggttc aaaacatcaa tacctgatag tgaaatcgcg cttgcaatcg
     4321 ttgactgtgg agggactctt agatgtggaa tttatcctaa aaaagggata ccaaccatca
     4381 atatcgttcc cacaggaaaa agtggcccat tagcacaata tatcactgaa gatatttatg
     4441 tttcagcagt agggttagaa caaatacagc cactaatgga aactgatgat aacaaagaaa
     4501 cagtaaatac tgcatctcca gataaaaagt acaaatatga tacttctaaa aagataaccg
     4561 agcaaaaagc agagcagcaa aactggatag aaaaaattgg aatgggcgcg ggaaaagtag
     4621 ttgctacatt caatcaatca gcaagagaag cagtagatac gatgatccgg acagtgatcc
     4681 cttttatggc tttcgtttcc cttttgattg gtgtgataca aggttctggg atcggtaatg
     4741 tatttgctaa cattatggca cctttagcag gaaatgcagt tggattaaca atcattggct
     4801 ttatttgttc actgccattt ttatctcctt tgcttggtcc aggggcagtt attagccaag
     4861 tgattggaac attgatcggt gtagagattg ggaaaggaaa tattcctccc caattagcat
     4921 taccggcact atttgctatc aatactcaaa atgcatgtga ttttattcca gtcggtctag
     4981 gtttggaaga agcaaaagct gagacagtag aagtaggtgt accatcagtt ctttattcaa
     5041 gatttcttaa cggcatccca cgagttatca ttgcttgggt agcaagtttt ggattatata
     5101 gctg
//
LOCUS       DACDPS010000121         5055 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.121, whole genome shotgun sequence.
ACCESSION   DACDPS010000121 DACDPS010000000
VERSION     DACDPS010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.121"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            172..516
                     /locus_tag="IWJ07_002534"
     CDS             172..516
                     /locus_tag="IWJ07_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAP9334062.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            517..936
                     /locus_tag="IWJ07_002535"
     CDS             517..936
                     /locus_tag="IWJ07_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAP9334063.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1028..1378)
                     /locus_tag="IWJ07_002536"
     CDS             complement(1028..1378)
                     /locus_tag="IWJ07_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334064.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1344..2675)
                     /locus_tag="IWJ07_002537"
     CDS             complement(1344..2675)
                     /locus_tag="IWJ07_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAP9334065.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2931..3497
                     /locus_tag="IWJ07_002538"
     CDS             2931..3497
                     /locus_tag="IWJ07_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9334066.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3642..3729
                     /locus_tag="IWJ07_002539"
     tRNA            3642..3729
                     /locus_tag="IWJ07_002539"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3674..3676,aa:Ser,seq:cga)
     gene            4063..5037
                     /locus_tag="IWJ07_002540"
     CDS             4063..5037
                     /locus_tag="IWJ07_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP9334067.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT         1829 a          844 c          972 g         1410 t
ORIGIN      
        1 ataaaaaaag cgttagcaac tatgcttaaa aagcatatcg actgaataaa ctaagcattg
       61 gacaggctga aagactacat ttaaaatgaa atgcgaagaa acaatagaaa ataaagttgc
      121 cttagaacca tagttgaatc acctaacagt cgtgaaggga gaataagaga aatggcagaa
      181 aaacaaaccg caggacgaga tcaattagga gattttgcac caaaatttgc agaattgaat
      241 gatgatgttt tatttggaca agtatggtcc agagaagaat tatctgctca tcagcgtagt
      301 atcatcacca tatctgcatt aatttcagga ggaaattttg aacaattgcc agctcatctg
      361 aagattggca agcaaaatgg cattactagt gaagaaatag cagaaatcat tacccattta
      421 gctttttatg taggatggcc aaaagcttgg tcagctttta gtttagcaaa agaaatctat
      481 ttagaagaaa atagcgaaga taagaaagag gcgtaaatga cgacaaaaca cgaagaaata
      541 cacaatggcg tgatttttcc agcaggtgaa aaaaacgaag cttacgctca agtatttacc
      601 gggcaaagct atctgaatat gttagtgaat gatcccgaag tttctgttgg tgtaggtaat
      661 gtaacttttg aaccaggttg tcgcaatcac tggcatgtcc ataaagacgg ctatcaaatc
      721 ttacttgtga ccggaggaga aggctggtat caggaagaag gaaagccagc aagacgatta
      781 atggctggag atgtcgtcgt aacgcacaaa gggattaaac attggcatgg tgcgacaaaa
      841 gacagttggt ttgaacattt ggcgatcact gctggtaatt cagagttttt agaacctgta
      901 acagatgaag tgtatgaagg attggaaaat caataatgtc aagcttgaac agctcctgaa
      961 agtagatttt aatcgaactt tcaggagctg cttttttgat tcagtctttt ataaatagaa
     1021 aatagggtta ataataggaa tgagaagcac gtcgaaagat cttaatattg ataatcggct
     1081 gggcatacat gatttccacg tcatcaatca aactacctaa cagatgataa tcttcccgat
     1141 catgtcgggg attacctaat agttcttgat aatagatttc catactaggg tcccgaggat
     1201 tgttcagcaa atcagcaagt acatctaaat cttctggctg agtttctgcc gtaccctgta
     1261 tctctatgaa aagcccttcc tcagcaaatt ctactccaat acgtatttca gttatttcat
     1321 gggaatataa ataagtaaga agctcatcga tgattctttt tgataattct ttttccaagt
     1381 gtcttttttt cctttctgac agcggtaaag ttatctagta tcggtacaag gatacctgca
     1441 acgaaaccag ttgaaaaacc actgttatat agatttagtc ctccgtgcaa agtgctgacg
     1501 ttatggacaa gagcaatatg tagtacacca gctattacac cgtaaaaact gccgtaaaaa
     1561 ccgcttatgg gcgcgagacc tgtcccaaac acagctgcaa caagcatgcc agtactggtg
     1621 atctcatgaa aagttccaaa caaagaagca ataaatattc cgactagtat cggaaaactg
     1681 ttttttaaat gacaaccaaa agcactgaaa cccaccgcag ataaaatagc accgatgaca
     1741 ggtccattta actgaccgcc aaccagcaaa acataagaag acagcatcag accaactaag
     1801 cctatgttga tcaaagtagc accaatacca gaattagcaa tgaaatcagt tgtcaatttt
     1861 ccagatgaat cgaaaatttc tcgaatcccc aaaagccgaa aggaattata atagaaacca
     1921 attccgactg tcagcgaaaa aaagccaaaa ataatccaaa tgagaggaaa gtgatattca
     1981 ttgaaaacca atgtattttc gacgattgaa tagctgaata acctcaaaac agcagtaaaa
     2041 gtcatcgcaa tcaacccaga ggtgaatcca atattataaa gtgtgaatcc acgatggaaa
     2101 actaaagttt gagcagctaa gggcggaaga agtagaccaa ttgctatgcc agctaaattc
     2161 cccaataaaa tacctatcag taaaggaaaa cgcataccaa aagtgatata actaacgacc
     2221 ggtgataatg cgctaccaaa cagtccgatc atgatgtact gagaaaaagg tttattgaca
     2281 aatacagcgt atagatacac acctaaaatg attgagatag aattataaag gtttttgcca
     2341 aaaaaagaga aaccggtaac tgttagaatc gaagcaaata agggaccgtt taaaatcacc
     2401 ttgtgccgat aagctaaaag cacagaaagt aaggtcaaaa tccctacatt tatgaatgcg
     2461 cttccgaaac cgcctaaagc aaaataatct gtcaaaagat tgcttggcga ggtaagaata
     2521 gttagcaatc cactccaatt atcagtagga gtattagaaa gcaagccaat aaaaagaaaa
     2581 aaaacacctg taaaaccaag ataaaaatac tttcttttct gtgaaacgat tcgtttttga
     2641 ggatgaatat aaataacgtc agtttcgttt tccattacac accttctttt tcattttctt
     2701 taactataac ttgaaatgaa agatttggac aactatcatt taggtcaaaa ctaaaaatgt
     2761 aagggaaccc attgaattta accaaatttt tttaattttg caaaaaaaag ttaaagaaga
     2821 ctgaaaaaaa atttaaaata taacgttttg agtaaaatgt ttgcaaaaaa tgctaaaaat
     2881 agggtacaat aaaacaacat atactgcagt aaagaagtgg gggagtttta atgagaagaa
     2941 aagtctatac caaggatcaa attctaaaag ctgcttatga tgttgttgca aaggaagggt
     3001 ttaaaggatt tactgcgaga aatattgcta aaaagatggg gatttcgaca cagccgattt
     3061 atctggaatt caaaaacatg gaagatttaa aaaatacgtt gctggattct atctttgaac
     3121 aactaaaaga agatgttttc cctattgaac gtactggtga taaaatcatt gatctcggtt
     3181 tgaactatat ctactttgca aaaaagcatc atagtcttta catggcattg tacattgatg
     3241 aacatgggga cggaaagaaa atgcatgatt tttcatacaa ttatttccgt gaaatagtta
     3301 aagaagatga aagatacaat caattatcgg atgaatatgt cgatgctctg catactggta
     3361 catgggtagc agtgactggc ttagcgtcat tgatgtcatc aaacatcatg catccaacag
     3421 aagaacaaat cattgcactg atcaaacgga caattgactc gattttagca ttagataatc
     3481 ctgaaataat cagatagtgt tctatcagta tgaatttgat cggtaagtcc gttggctgca
     3541 aactgtcaat ggactttttt gctaaaaaaa gcgatttctg ttatttatct attagaaata
     3601 ggcttgacat ttcgtgaaaa aagtttaaaa atagtactta cggaaggttg gcagagtggt
     3661 aatgcaccgg actcgaaatc cggcgaaccg gctaataccg gcgcgcaggt tcaaatcctg
     3721 taccttcctt atatcaacga ttagaggttg tgaagaagct gtcctgcatc aagtaataag
     3781 aacggccaaa caaaaatagc ttttcctatt ttttcgtttg attgggctta ttaccgcaga
     3841 tgcaagcttt tgaacaccgt ttgttcggaa taaggggctg tgacaagact tttgtcacag
     3901 ccccttattg ttgtactttt aacctacaat attttttgat tccccctgag tactattttt
     3961 tataaatgct agtaaatcaa tcttctttgg cctttttttg ataaaaaaaa tactagatgt
     4021 aagataaaag tgtaaaaacg atttgtttta ggacgggaaa cgatgtgtac cgcaatcact
     4081 tatgcaacga aagaccatta ttttggaagg aattttgatt atgagatgtc ttataatgaa
     4141 gtggtcacta ttactccaag aaattatcgt ttcgatttta gaaaggtaaa aaatttagat
     4201 aaacattatg caatgatagg aatagcagct ggggtatcca actatccact ctattatgag
     4261 gctacaaatg aaaaagggtt gagtatggca ggactgaatt ttcctggcaa tgctgattat
     4321 aaagaattac aagaaggaaa agataacgtt gcaccatttg agtttattcc atggatacta
     4381 gggcaatgct caaccataga cgaagctaaa gaattattgg ctactatcaa tctagtcaat
     4441 atcgatttta gtgaaaaact acccttgtca cctttacact ggttattagc tgataaagaa
     4501 aaatcgatag taattgaaag tatgaaagat ggacttcatt tatatgataa tcctgtcggt
     4561 gtgttgacaa ataatccgcc atttgattat caattattta atctgaacaa ttatcgctcg
     4621 ctatcaaatg gaacaccaga aaatcatttt tcaaaccaga tcagcttgaa tgtttatagt
     4681 cgtgggatgg gaggattggg cttacctgga gatctctctt ctgtttctcg atttgtaaaa
     4741 gccacattta ctaaaatgaa tgctgcatca ggtgattcag aatctgaaag tatcagtcaa
     4801 ttttttcata tactaggctc agtcgagcaa caaaaaggag tatgcgatac tggagaaaga
     4861 aaatacgaat atacgattta ttcttcttgc tgtaacgttg ataaagggat ttattattat
     4921 cgtacgtatg aagacagtca aattactgct gtaaatatga ataaagaaga cttggatcgt
     4981 catgaattgg tcagttatcc agtcataaaa gagcaacaga taaactatgt caattaatta
     5041 atgaatagat ccgtt
//
LOCUS       DACDPS010000122         4992 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.122, whole genome shotgun sequence.
ACCESSION   DACDPS010000122 DACDPS010000000
VERSION     DACDPS010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.122"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(113..802)
                     /locus_tag="IWJ07_002541"
     CDS             complement(113..802)
                     /locus_tag="IWJ07_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP9334068.1"
                     /translation="MESEDLLSRISEVHPNQVWLDKEKVEKRYIYLFFKRMIDIVGSI
                     IGLILASPLMFYVAYRIRKEEPGSPIFFCHTRVGKNGKTFKMYKFRSMCLDAEEKLEE
                     LLDQNEIEGAMFKMKEDPRVTKIGKIIRAKSIDELPQLWNVLKGDMSLIGPRPPLERE
                     VAEYTRYDLQRLYVKPGCSGLWQVSDRNNVSFHKMVELDLEYISHRSLMYDLRLILKT
                     FYVIVKPNGAY"
     gene            complement(847..1611)
                     /locus_tag="IWJ07_002542"
     CDS             complement(847..1611)
                     /locus_tag="IWJ07_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAP9334069.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEWLFFELLELGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQITAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(1639..2337)
                     /locus_tag="IWJ07_002543"
     CDS             complement(1639..2337)
                     /locus_tag="IWJ07_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAP9334070.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAR
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILDEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLNKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(2349..3128)
                     /locus_tag="IWJ07_002544"
     CDS             complement(2349..3128)
                     /locus_tag="IWJ07_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAP9334071.1"
                     /translation="MEETINLEEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDALGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(3144..4088)
                     /locus_tag="IWJ07_002545"
     CDS             complement(3144..4088)
                     /locus_tag="IWJ07_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9334072.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVAIVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(4134..4913)
                     /locus_tag="IWJ07_002546"
     CDS             complement(4134..4913)
                     /locus_tag="IWJ07_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334073.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSYVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISAAYATKAQTVAQQMGYSNISY"
BASE COUNT         1301 a         1059 c          867 g         1765 t
ORIGIN      
        1 ttagattttc tacaagtgat tcaaaccctc cataattaca gttattactt ttgaaacgat
       61 aatatgaaca tgtgtaatct tttttattaa caatccatca ttctatccct aactaatatg
      121 ctccattggg cttgacaata acataaaacg tttttaaaat caacctcaag tcgtacatta
      181 aacttcgatg actaatatac tctaaatcca actcaaccat tttatggaaa ctaacattat
      241 ttctatcact aacttgccac aaacctgaac aaccaggttt tacatacaag cgttgaagat
      301 catatctcgt gtactcagct acttccctct ccaaaggtgg ccgtggacca attagactca
      361 tatcgccttt taaaacattc catagctgtg gcaattcatc aatgcttttg gcacggataa
      421 ttttacctat tttagtgaca cggggatctt ctttcatctt gaacattgca ccttctattt
      481 cattttgatc aagtaattct tccaattttt cttcagcatc taaacacatc gaacggaatt
      541 tatacatttt gaatgttttt ccatttttac caacacgtgt atggcaaaaa aatatcggac
      601 ttcctggctc ttcttttctg attcgataag ctacatagaa catcaaagga gaagctaaaa
      661 tcaaaccaat aatgcttcct acaatatcaa tcattctttt aaaaaataaa tagatatatc
      721 gtttttcaac tttttcttta tccaaccaaa cttgattagg atgtacttca ctaatacgag
      781 ataataaatc ttcactttcc atactttccc tctctcctca tttccttttt atctacgaat
      841 aaagaactaa aataaaccaa acttcttctt ttttacctct tcatagactg gataactgat
      901 ttcatccccg ttgatcagat cctttgcgac ttgtttcatc tgtactactt tttccttacc
      961 aaagtcctta gcaatctgct catagcactc ttttaagtaa aaggtacgct tagagacccc
     1021 atgggcatca gaagcagcca tctgcacgag attatgtttg accattgttt tggctgtttt
     1081 ctgaatgtct ttgccaaata caccaacata acttggcgca gtaatttggg ccaaacatcc
     1141 catatctagg aacggaagca aatgatttgg gtcttctcga aaatgcgcgt tgcgttctgg
     1201 atggacgatc actggtgtct ttcctagttc taacaactca aaaaaaagcc attctgcata
     1261 taacggcact tccagtgtcg gaaattcgat cagaagatat gtatcatcta aatctgtaaa
     1321 taaaatttca tctcggtgga tgtcttcgat gagcgttccg gtaatacgta cttcttgtcc
     1381 ttccaataac gtcaaaggca actgtctttt ctctaattcg gcttgtaacg aagcgactag
     1441 agaaatgact tgtgattttt cattgctgta ttttccatta ttgtggtgtg gtgtgcataa
     1501 aatatgcgtg atgccttgct gaatcgcttt ttcagccatt gcaatacttg cttctaagtt
     1561 ttctgccccg tcatcgattc ctggtaggat atggcaatgt agatcgatca tttctcttgt
     1621 ctcctctctt cttattcctt aatttccgta gtaataacca gcatctttgc tatgttctgc
     1681 tccgttgtat acaacaccta aaactcgtgc ttgtaccatc tctagcagtt cttttgcttt
     1741 attcaatgat tctttacgtg atgtattttc acgtacgacc aataaagtgc catccgtttt
     1801 tgaagacatg atctgtgcat ctgttacggc tactactggt ggcatatcga atatcaccac
     1861 gtcaaataac tgtcttgctt cgtctaggat ctgatccatt cttggcgaac ttaacaattc
     1921 tgatgggttg ggtggcttag gtccactagg caaaatcgac aagttctcga ctggcgttcg
     1981 ttggattaca tctgctacac ttcctgatga actaagtgct gtacttaatc ccctggcatt
     2041 gttcagctgg aatgtcttat agacgacggg tttacgtaaa tctgcatcga ctagtagaac
     2101 tcgttgaccg gatttagcaa aaacgactgc aatgtttgct gcagtggtgg attttccttc
     2161 tccaggacct gatgaagtca cgacaatcgt tttgatttgc tgaccagctg cggaggcaaa
     2221 ctggatattc gtccggatcg tacgatattg ttcggcgatt ggtgaagatg ggtttgttaa
     2281 tgtgatcaaa ctgactgcat gagtttgtgc tgctttttgt ttctgtgttc gtgccatttt
     2341 tcttcctcct agactcttga gcggcttctg cgtgatgggg ctgttttatc gtttgtagct
     2401 tttggtcgtg caggagctgt tcctttttta cggataccag cgttcaattc tttcgctgac
     2461 atttctggaa ctgttcctaa gattgggaat cctaatgcat ccgtcacata tttttcatct
     2521 ttcactgtcc gatcaagtaa ttcgaataag aatgccagac ctacgcctac catcattcct
     2581 aatactaagc caatcactag attcaattta ttatttggcg aaacaggtgt cgtattggca
     2641 actgcacctg aaataatcga gattttatcg acattcatca cgtcttttgc gttctcttgg
     2701 aaaacttgtg aagttgtgtt agcgatctgt tgagcagtca ctgcattcgt gcttattgcc
     2761 tggatcgaga acatttgtga attttgtgac tgattgacgg agatcgcatc tttgatttgt
     2821 ccagcagtca ttttcacatt gtcttctgtt tctaaacgat ctttgacttc atttagtact
     2881 aaatcactga cgatcatatc tttatacgta ttgatcattt gcaggttcgt attgacatca
     2941 ttgacatttg ctgtgtctga ttgtggcagt gtcacgatca attgtgcttg tgaactgtat
     3001 tttggtgtca agatgaaaaa tgttacgatt cctgagatgg ccaagccagc aaacattgag
     3061 atgatgattg ttaccaagtg tttgcgtaat gtttcaaaaa tttcttccaa gttgattgtc
     3121 tcttccattt tttctccact ttcctattta ttctctagat tcatttgatc ttttaattct
     3181 ttttttacac gatcaagttc ttgatcactg actttttgat aggagatatt atcttgcata
     3241 aagccttctc cttgcagctg atcttgcttg atcgtaccaa atgcctcccg ataatctaaa
     3301 gcgatttttt tcatttcatc aaagctcatg tctgttttca tattcgactc tagagcattc
     3361 aaaacggatt gatagttgct gacaccgttt aaactcaata ctttttttgc tacaccttct
     3421 acgatctttc tttgacgctc ttgtctacca tagtcaccat ttggatcttc ataacgcata
     3481 cgagaataag ataatgcctg atccccgttc aatgtgattt taccgattgt aaagtcatag
     3541 ccatcttgtg agaaagtgag gtcattattg acttcgatac caccaactgc atcaacaagt
     3601 tcctttagcc ctttcatatt gatagagaca tagtgatcga ttgggatgtc tacatatttt
     3661 tgtacggtat ccatcgccat ggcagctccg ccgaatgcgt aagcgtggtt gatcttgtct
     3721 tttgtgtcac gacctacgat gtcgacatac gtatcacgag gaatgctgac gatcgttgtc
     3781 tgtttgtctt caggactgac tatggcaacc atcatcgtat ctgatcttcc ttgatcgacc
     3841 cgacctaaat ctccagtatc aatccccatc aatagaacag agaaagattc ttgtttgtcc
     3901 agatcaactg gtttcgttcg ttctccttgg gttcgttcca ctgattcgta agttttctca
     3961 atagaatgtg agagatccag atataatttt gctcctactc ctacaattgc taatactaat
     4021 acgataagaa ctgctaaaat aatcagaaat atttttttcc ctcttgattt tcttcttcgt
     4081 cttgccatag tttcctccgt ttgctaacct tgcttgaatc aacgttttaa ctgtcaataa
     4141 cttatattgc tatagcccat ttgctgagca acagtttgtg cttttgttgc gtatgctgcg
     4201 cttatttttt cattccggat ctgctctatt tgactgagcg ccgtcaaata ttgatcgtaa
     4261 gtagcggtgg cagtcacttg accatccttc aacatcgtat cgaataactg atctagttgc
     4321 gtgacttgcg cgatctgagt atcagcgaat cctaaatatt gcataacaac cgttttccaa
     4381 ggactatctt taaaaaagct catattttca cgtacacgag cgacatctgc acttgtcacc
     4441 ttttctttga tgatgacatc gtctacagct tgctgccagt ttgatacatt tttagtaaac
     4501 aaatcgttga cagctgtctg gatcttcagt tttgattctg cactatctaa ctgtgtcacg
     4561 attgctttct tctcatcttg gattgcaaca gcttctttag gcaattctga ttctttgatg
     4621 tcgtaatcat ctgctgaaag tttgacatag gatagcttat ttgacaaact tgtgattgtt
     4681 tcttctgtta tttcagggtt tagaaaaatt ttttcttttg tactatacag cttgtctact
     4741 tcttttgata aagaagtcaa ggtgtctttt tgattggtga tggcagattc tgcataattg
     4801 atccgttgct gatgttgccg ctgattattg atatggtaac caaacaaaca gatcgcaata
     4861 gcacctgcac ctaccgccac agcagtcaat gacttgacga atggcttttc catagattca
     4921 ctcctaagta ttgatgatag ataaattaag atataattca cttacataaa aatgacagct
     4981 ttttatttta aa
//
LOCUS       DACDPS010000123         4977 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.123, whole genome shotgun sequence.
ACCESSION   DACDPS010000123 DACDPS010000000
VERSION     DACDPS010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.123"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..658
                     /locus_tag="IWJ07_002547"
     CDS             <1..658
                     /locus_tag="IWJ07_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9334074.1"
                     /translation="SLQNQAYQSIRQQIIYADLEPGKKVSEKKLEETLSIGRTPIREA
                     LIQLRHQGLVDTIPQSGNYISLIDLDLAKNARFVREHLERQIMLECCAKMNNQKKQVL
                     ETIIAEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLEDNNTHLERFRWLRV
                     KTEGLKWEKIMEQHHQLFKALTMQDPEEANFLTSLHLHLMLDEQNVVLTTHPKYFKNI
                     "
     gene            complement(1494..2450)
                     /locus_tag="IWJ07_002548"
     CDS             complement(1494..2450)
                     /locus_tag="IWJ07_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334075.1"
                     /translation="MLILANQASTFVQTQQLRGRGLRLGAQNKVTNIWHLAIGLPGVP
                     LSEQPDLKRMLRRLGNISGLTFESSPKIESGGDRFALPLEYTTTTMETYTQSMFYFSC
                     ERSRLKEQWSTALADGWHLSMPIVVRKVDQVKQKQETSLPIVVKNIEPLSFWSAIFKG
                     NLLEYFQIRRRQKLWEKECARKKSLAICVQTTLQRLGEVSDRKVIVAADQQEFRCYLE
                     NAGYREGQLFTEAMRELLAPLENPRYLIEGKKIVLTVPERFGRNKQIANLFLNELNQS
                     SLPKCQLVYTRNPEGRQKLVTYRMRQVFQTRDEVIIQKNIWT"
     gene            complement(2559..2804)
                     /locus_tag="IWJ07_002549"
                     /pseudo
     CDS             complement(2559..2804)
                     /locus_tag="IWJ07_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2949..3371)
                     /locus_tag="IWJ07_002550"
     CDS             complement(2949..3371)
                     /locus_tag="IWJ07_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334076.1"
                     /translation="MVKEEVLCPHQDFLYLIPANQETTQHYFTFLEKQNELVEALMES
                     QETVDYLLSLEFVQHPLEHSKEIYENFELFTSALIYLNHHNYELIPEHWQLLGSSKQQ
                     GKKASVPSLGKKELITIYQQMFEDQPELPIFYQLKKAG"
     gene            complement(3510..3940)
                     /locus_tag="IWJ07_002551"
                     /pseudo
     CDS             complement(3510..3940)
                     /locus_tag="IWJ07_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347687.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            complement(4256..4519)
                     /locus_tag="IWJ07_002552"
     CDS             complement(4256..4519)
                     /locus_tag="IWJ07_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334077.1"
                     /translation="MFIKYGADTVATMFTEEYQVDYYEVIDKCVHFVYEKVWNFMNFA
                     ISEEILRVQENRTGIFTRDRKLNLIAHYIRNRWSSIPNFNYKK"
BASE COUNT         1577 a          984 c          763 g         1653 t
ORIGIN      
        1 tagtctccaa aatcaagctt atcaatctat tcgtcagcaa attatctacg ctgacttaga
       61 acctggaaaa aaagtatcag aaaaaaaatt ggaagaaacg ctttctatcg gtcgtactcc
      121 aatacgtgaa gcattgattc aactccgtca tcaaggactt gttgatacaa ttcctcaaag
      181 tggaaattat atctcattaa tcgacttaga tttagcaaaa aatgctcgtt ttgtcagaga
      241 acatttagaa cgacaaatta tgctagagtg ttgtgccaag atgaataatc aaaagaaaca
      301 agttttagaa actataattg cagaacaaga aaaagctgtt agtcaaaaga atgctcgtag
      361 tttttttcat aatgataatt tatttcataa agcttgtttt gaaattgcag aacgggaaga
      421 agtatggcaa tggttggaag ataataatac gcacttggaa cgatttcgtt ggttacgagt
      481 aaaaacggaa ggattaaaat gggaaaaaat tatggaacaa catcatcaac tatttaaagc
      541 cttgaccatg caagaccctg aagaagctaa cttcttaaca tccttgcatc ttcatttaat
      601 gcttgatgaa caaaatgttg tattgactac tcacccaaaa tattttaaaa atatataact
      661 gctaaatttc ttttttttaa tttatttaca tttctgatta ctacagaaaa ctaactactt
      721 tttttatgta aagttctgtc aaatatagtc actttatatt actttctctt gagtaaaaaa
      781 acatgtgagg tcgtaaacaa agattgaact atctacattc atactctata atctgtttat
      841 ttgcagactg ataatagcaa aagaaaactt ataatcaaat aatatcaaca tttgatcaca
      901 taatttgaga ttgaatgctg atttacctat gttttacgta atggtcttta gttatttttg
      961 ctcataaaat gcataaaaca cgcgttttta tgtgtgcttg aaatttcttg gctctataat
     1021 ttctaagact gataaaaata ttctatcggt tctttttgca aataaaaaaa catatagaag
     1081 ctccattaga ttgaatcacc acaacacaat caaaggagca ttccgtatgc ttgattttca
     1141 ttctataaaa ctacttttag gaatcaaaga aaatcacaac ggttcaaact aaaagcaaga
     1201 atatccgaag taccattgtt tcatcgatca tgagaaaagc attcacctct gtctgcagat
     1261 gttcttatat cgctctgtca gtcctattaa taaagtactg ttttgttttc aaaaaaacta
     1321 ttacgagtac tttttaagag aatgccttgc aaatattcat cacacaccgt tatataagtc
     1381 aaatagtttc tttcagtcgt caagaaaaga ttctcctgtc cttggcatta tcagttaagt
     1441 agcaacttaa ttctcggact tgttctactc tagcaaaatt tattttcatc aacttacgtc
     1501 caaatgtttt tttgaataat tacttcatcg cgggtctgga agacttgcct cattcgataa
     1561 gtcactagct tttggcgtcc ttcaggattt ctagtataca ccaactgaca tttgggcagc
     1621 gacgattgat ttaattcgtt tagaaaaagg ttagcgattt gcttatttcg gccaaagcgt
     1681 tctggtaccg tcagaactat ctttttccct tcgattaaat accgcggatt ctccagcggt
     1741 gctaaaagct ccctcatagc ttcagtaaaa agctgtcctt ctcgatagcc agcattctca
     1801 agatagcatc taaattcctg ctgatcagcc gctacgatta cttttctatc actaacttca
     1861 ccaaggcgct gcaacgtcgt ttgaacgcat atggccaagc tttttttgcg ggcacattcc
     1921 ttttcccata gcttttgtcg cctacgaatt tggaaatatt ccagcaagtt acctttaaat
     1981 atagctgacc agaagcttag aggctctata ttttttacta ctatcggcaa tgaagtttct
     2041 tgtttttgct ttacctgatc gactttccta acgacgattg gcatagatag gtgccagcca
     2101 tcagctaaag cagtactcca ttgttctttc aaccgacttc gttcacaaga aaaatagaac
     2161 atcgactgag tataagtttc catcgtggtg gtcgtatatt ccagtggtaa agcaaagcga
     2221 tcgccaccac tctcaatttt tggtgaagat tcaaaggtca gacctgaaat attgcccaaa
     2281 cgccgtagca ttcttttcaa atcaggctgt tcagacagag gaactcccgg taatccaatc
     2341 gctaaatgcc aaatgttcgt tactttattt tgtgcaccta ggcgtaagcc ccgccctcgt
     2401 agttgttgtg tttgcacaaa agtggaagct tgattagcca agatcaacag attaattgca
     2461 ggacaatccc agccttctcc tagcaaacta acggttccta ccaaaatatt caacttcttt
     2521 tcggttaata ggcgagtaac tgcttgcatc aatgcttggc ggtttttgtc atccaccgtc
     2581 aaataaaaat aacccgacat ataaggatgt tcattcatac ccgtcgctag ttctgccagc
     2641 tcaacagtta ctagcatttt cggtaacagt aagaactctc cacagatggc accgatttgc
     2701 tgagtgttac ctaatagacg aacaacttca tcaaacaaag gaaccacgcc ataagctaag
     2761 gtaccatcac tgcctgctaa tacctccttg caaatacgat ccatcaaaat gaccgctgtc
     2821 agttcttgct tcagctgaac ttcttctttt aacaaaattt gacaaactgc ttgctttttc
     2881 tcaggaatcg cctgactacc aacagtgtct ggatactctg gaaacaaatg cagcgactca
     2941 tcaaacaatc atccagcttt tttcaattga taaaaaatag gcaattccgg ttgatcttca
     3001 aacatctgct gataaatagt gatcagctct tttttaccta aagatggaac tgaagctttt
     3061 ttaccttgct gcttagaact gcctaatagc tgccaatgtt cgggtatcaa ttcatagtta
     3121 tgatgattca agtaaattaa tgcactagtg aacagctcaa aattttcata aatttccttc
     3181 gaatgctcaa gcggatgctg aacaaattct aaagaaagca aataatccac cgtctcttgg
     3241 ctttccatta acgcttccac cagctcattt tgtttctcta aaaaggtaaa ataatgctga
     3301 gtagtttcct gattagccgg aatcaaatat agaaaatcct gatgaggaca tagaacctcc
     3361 tcctttacca actcgggaat gctaatttct tcgtcaatca ggccatttta actcaatata
     3421 ttgcttccac tccgtctgag agctatcaat cggaggcgtg gctgtcaagg aaatcgtcaa
     3481 cagattcggt tgttttttct tcaagtcaat caacaattca ccccagcttt tttttaaatg
     3541 atgagcttca tcaaaaaact aacagctcca gctgatcacc ataagttggc tcctccttca
     3601 gtcggttgta aatggtctga tacgtttcca ctgtaatatg actaggatgt tcaatcgccc
     3661 cagaaagata gcaactatct gctggattta ctaagaaata ttcctcgatt gttgttaacc
     3721 actgtttctt caatattagg gaaggaacca aaattaaggt tttacaatcc aaacgtcgga
     3781 tcatctcaat tcctaaaaag gtcttgccag cccctggtgg agccactaga tggactgaat
     3841 tgttatttaa ataagtagca aagttagtca atatacgctt ctgatagcta cgccactgaa
     3901 ttttttcatc agatagccac atcccaggtc cccctctcac gtattttctc tatcataatc
     3961 taaatgaata cagcaaaaaa aagatttctt caagaatatg tgtttttata gtaatatttt
     4021 acttactcta aactcaaatg aaaaggtaaa aaaactggta gtacaatctt ttaaattctc
     4081 attgttttgt acgttcttta ctatcaaaaa aacaaaaaaa acgcgtttct acgcgtgacc
     4141 aaaattttta gctctataat ttctaagact gatagaaaat cattctgttt ttgaagtctt
     4201 ttttcataaa tactacttga tttattgtgt atattgtata aaagattgtt cataattatt
     4261 ttttataatt aaaatttggt attgatgacc agcgattccg tatataatgc gcaatcaaat
     4321 taagctttct gtctctggta aatattcctg ttctgttttc ttgtactcgt aagatttctt
     4381 cacttatcgc aaaattcata aaattccaca ctttttcata aacaaaatgt acacatttat
     4441 ctattacttc ataatagtct acttgatatt cttctgtaaa catggttgct actgtatctg
     4501 caccatattt gataaacatt tgggctactc ccttaaattg agaaaagccc attagtttat
     4561 ctactaatac ttctaaaatt tcttgctttc ttctttaaca tcgtcccgta aggaatcatg
     4621 tagtatgctg gaggaagttc attttctaaa taattgtatc gcttcaattg ctcaaaggct
     4681 attctctaca attaattaca ccaattactt tatcttcact tatataagat ttctaaacat
     4741 ttttcgtcaa atgaagagaa aatatttttt taaatgatct tgctcaagat ttgtcaacga
     4801 atctttgaat tcgttttgaa gttctgagga aggtatcatt taaaaactca atctgacttt
     4861 catcatttcg tttactccca ttttgatcac cttctaccaa caatttatta aagtgattat
     4921 actaggtttt tattttttta attcacaatt gtttattcac ttcatgaaca attaaac
//
LOCUS       DACDPS010000124         4932 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.124, whole genome shotgun sequence.
ACCESSION   DACDPS010000124 DACDPS010000000
VERSION     DACDPS010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.124"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..891
                     /gene="gnd"
                     /locus_tag="IWJ07_002553"
     CDS             <1..891
                     /gene="gnd"
                     /locus_tag="IWJ07_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAP9334078.1"
                     /translation="VIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAYQ
                     EISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRAE
                     EFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGAS
                     GSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEIM
                     EDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFSA
                     KVVSALRNGFGGHEIVEKGE"
     gene            893..1894
                     /locus_tag="IWJ07_002554"
     CDS             893..1894
                     /locus_tag="IWJ07_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP9334079.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            1891..2175
                     /locus_tag="IWJ07_002555"
     CDS             1891..2175
                     /locus_tag="IWJ07_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9334080.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            2208..3005
                     /locus_tag="IWJ07_002556"
     CDS             2208..3005
                     /locus_tag="IWJ07_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9334081.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            3019..3852
                     /locus_tag="IWJ07_002557"
     CDS             3019..3852
                     /locus_tag="IWJ07_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9334082.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            3870..4727
                     /locus_tag="IWJ07_002558"
     CDS             3870..4727
                     /locus_tag="IWJ07_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9334083.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            4749..>4932
                     /locus_tag="IWJ07_002559"
     CDS             4749..>4932
                     /locus_tag="IWJ07_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9334084.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIV"
BASE COUNT         1577 a          803 c         1141 g         1411 t
ORIGIN      
        1 gtaattggtt tgggaaaaat gggattgaat ttagctttaa acttaaaaga gcaccatcac
       61 aaagtacaag gactcgacat ttcaagtaca gctgttagtc aggcaagaga aaatgacatt
      121 cctgcttatc aagagatctc tgaatttcta gctacttttg agcagaaaaa aatcatctgg
      181 ttaatgcttc ctgctggaga gataaccgaa aacatgttaa gaaaactttt tccaaaactc
      241 ggaccaggag acattattat tgaaggcgga aactcttatt ataaagattc aatccgtcga
      301 gcagaagagt ttcagaaaaa tgaaatcggt tacttggatt gtgggacttc tggaggaatc
      361 gaaggagcac gacatcaagc ctgcttaatg attggaggag ataaagaaac ttttctatcc
      421 gtggaacaat tattcaaaga tgtagcaatt gaaagaggat atctgtatgc tggtgcatcc
      481 ggaagcggac acttcttgaa aatgatccat aatggaatcg aatatggaat gatgcaagca
      541 atcggtgaag gattccaact tgtacaaaaa agtgagtatg cttttgactt ggaacaagtc
      601 gcacgtgttt ggaatcatgg ttcggtgatt cgcggctggc tgatggaaat catggaagat
      661 cagttaaacg aacatcccaa tttaagtaat tatcggggaa tcgtttctgc atcaggtgaa
      721 gcaaaatgga cggttgaaac agcgctagaa atggctgttc cagttccagt aattgcaact
      781 agtttgttca tgaggaatct ttcacaggaa gaagatagtt tttcagcaaa agtagtttca
      841 gcactaagaa atgggtttgg cggacatgaa atagttgaaa agggtgagta aaatgccaga
      901 ttatcatata gcaatcgtta attccagcag tttcggaaga gtctttccac accacttgga
      961 aagattaaag tcagttggag acgttcattt ctttactgtt gatcaagaga ttccaggaaa
     1021 agaactagcg gagttactta aaggattcaa tatcattatt gcaagtgtta cgccattttt
     1081 tcgacgagat ttttttgaaa acaaagatga actgctattg atttcccgac atggtattgg
     1141 ttacaacaat attgatttaa aagcagcaaa agaacaccag acaatcgtat ctatcatacc
     1201 tgctttagtt gaacgaaatg ctgtagcaga aaataatata actaatttgt tgtccatgct
     1261 tcgacgcacg acacaagcac aagcaagagt caaggacgat cgatgggaag atcgggcgga
     1321 gttcgtcggt aggacgctgt ttaacaaaac agttggtgtg attggtgcag gaaataccgg
     1381 aagcggagta atcgaaattt tgcgtaacgg ttttcgctgt gatgttctag cttatgatcc
     1441 ttacaaatca gatttataca tcgaaagctt tggtgcaaaa aaagtagaac ttcaagaatt
     1501 actggaacaa tcagatatca tttgtttatg tgcgaatttg acagaagaga actatcacat
     1561 gatttctgat aaagaagtcg atcagatgaa agaaaatgtt tatctatcta atagtgcaag
     1621 aggcgctcta gttgatgaag aagcgatgat taatggtctg aaaagcggaa agatcgcagg
     1681 atatgccaca gatgttttag aagaagaacc gggacgtcaa gatcatccgt atttagcgtt
     1741 tgaacaagta gtgattacgc cacatatatc ggcctacaca agggaatgtt tagaagcaat
     1801 gggtgaaaaa tgtgtcagag atgtcgaaca gatcagtcag caccttcttc ctgaacgttc
     1861 tgtccagaca aagagcaact atataaaagg atgatgaaga aaatggtgaa cggtttaaaa
     1921 gtgaaaacag gccctcaatt ttatttatat gaagaaggtg gtatttcaaa agtttctgat
     1981 ctcttgaaat catacggcgc gaaaagagta ctggtcaccc acggaacggt atcatgggaa
     2041 aaggcgctac ctaaattagt atttttgaat gacgaaacga ttcaattttt ttatcatcgt
     2101 tattctggag aatgtagtta tgctgaagcc agacgaatag ctacaatcat caaaaaaatg
     2161 aaatcgattt cgtagttggc gttggcggag gaaaactgac cgatctagtg ggttatagtg
     2221 ccaatcttgc aagaatcgat ttcggagtca tccctacttt agctagtaat tgtgctccat
     2281 ggacacctct tgctgtcatg tataaggaga atggacaagc agaagggaaa acagaacact
     2341 tttacagaca agctgctttt tttataacag atccccaatt agtgattgat tcccctgttc
     2401 catatttcat tgccgggtta gcagatacac tagcaaaatg gtatgaatct gagctcatct
     2461 tagaacaaga ttttttgcaa gatgaatcat ttttgaagct agcacaagat actgcgaaga
     2521 tctgcaaaga agagattttg aatcattctg ctaaagcaat ccaagatatg gcacaaagaa
     2581 aactgacatc ggagttcaaa cagctttcag agattatctt tgctgttgct ggtttagttg
     2641 gaggattagg agataaatac gcaagaaatg tggcagcaca tgctatgcat gacgctatca
     2701 gtaaatatat tccggaaagc catcgttttc tacatggaga aaaagtagcg tatgggattt
     2761 tctatcaact ggcactagaa ggtcgctggg aaaaaataga cgagttatat cctttgtatc
     2821 gggaattaga actgccggtt tctctagaac aaatgggatt atttccgaaa gataaacaga
     2881 taatagataa aatggctgaa tttattgatt cgaaagagaa agtgcatttg attcctatag
     2941 aggtaacaca gaaatcactg atacagtcaa tgatggaact ggaaggttat atcagaaaga
     3001 tctgaaggag gaggacacat ggaaacatta agtgtagtac aaagtttgtt gattgcttta
     3061 tgggtagccg cggttatgtc ccgctggtta ggtggaggag ccacgttgac cttacgtttt
     3121 tctcctttga tgactggatt agtcgtcggg ttgatcatgg gggaagttgc acaagcgatg
     3181 attgttacag ccgctttgca aatgatttat atgggggttt tttctccagg aggatcaatg
     3241 ccagctgaac catctattgc agcagctatc gcagtaccag tcgcattatt aggggatctg
     3301 tcgccagaag cagcgattgc cgttgcggtg ccagtcggat tgctcggaag ttacttgtat
     3361 cagtttcgtt ttttcatcaa tactttttta ggtaaatata cagacaaagc tgtagaagat
     3421 ttgaatacaa aagctatggc ccgatcaatt attctttatc caacggttgc ctcattttta
     3481 ttattcgttc cgcttgtttt cattgcttta tatttagggg cgccattgat cgctgatgta
     3541 atcaaagctt tagagggaac agtcgttatc cacattcttg aagtagtagg tggagggtta
     3601 gcggcaatcg gtatcgcgac aactgtatac gttatcggtc gaaaagatta tctggtcttt
     3661 ttcttccttg cttattttat gagtgttgtt ttgaaatctt tagatattac gatggttact
     3721 tatgccattt ttgggattat tatcgctttg atctttgtcc agtcgcaaaa agggaaacaa
     3781 gtacagcctg ccacaagcaa taacaattca gcatcttttg atgacgaaga tgactatgat
     3841 gacggatttt aatttaggag gaatgggaaa tggctgaaat aatggataat caaacaggtg
     3901 ctcctgaaga aatcacgaaa aaagatgtaa caaaagccta tctgagatgg cattttgcaa
     3961 acgaaattcc tcactctttt gaacgttact tggcgccatc actactatac ggaatgatgc
     4021 caatcttgaa aaaactttat aaagatgaag actctttaaa agcagcatat aaacggcagt
     4081 tactgttctt caacactcaa ttaagctggg gtggcggtgt gattactggc ctaatgtctt
     4141 ctatggaaca gcaaagagca gaagaagagt atgaagaaaa agagatcttg atgcaggatg
     4201 acttgatgta taacacgaaa gcgggattga tgggggcact agcaggaatc ggagatgcaa
     4261 tcgattcagg aaccgtccaa tatattttta tcgcaatcgc ggtaccttgg gcacaacagg
     4321 gaagtccttt aggcgcactt tttcctttta tctgctttgg gttgtatcag gttttactag
     4381 gggtattttt cgcaagacaa gctttcacta tgggccgtaa tgctacaggc ctaaagcaaa
     4441 acactggtgt tcagacagcg attgaaatgc tatcagtttt agggttgttt atgatggggg
     4501 tgcttgcggg aaactatgta aaagtagaat cttcattgaa atttgcgata tccggacgtg
     4561 agtttgttat ccaggaaatt cttgatcaaa tcgtgccagg gatattacct ttagctgtcg
     4621 taatgggtgt gtacttgttc tatacgaaaa aaggattgaa agtcacacaa gcattattat
     4681 ggctgactgg aatattaatt gtgctagcag gaatcggtat tttataaaaa gaagggaacg
     4741 tgaataagat gggtgtagtc aatttagcgc gtgtggatga gcgtttgatc catgggcaag
     4801 taatggtaac gttatctcaa aaaagtggag tgaattccat ttttgttgta gacgaagttg
     4861 tagcaaaaga taaatttatg agagatcttt acaaaaatgc aggaagccgt acaggacaaa
     4921 aaacaattgt ca
//
LOCUS       DACDPS010000125         4826 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.125, whole genome shotgun sequence.
ACCESSION   DACDPS010000125 DACDPS010000000
VERSION     DACDPS010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.125"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            108..359
                     /locus_tag="IWJ07_002560"
                     /pseudo
     CDS             108..359
                     /locus_tag="IWJ07_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            452..1246
                     /locus_tag="IWJ07_002561"
                     /note="aph(3')-IIIa"
     CDS             452..1246
                     /locus_tag="IWJ07_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP9334085.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1656..1767
                     /locus_tag="IWJ07_002562"
                     /pseudo
     CDS             1656..1767
                     /locus_tag="IWJ07_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787626.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            1788..1871
                     /locus_tag="IWJ07_002563"
     CDS             1788..1871
                     /locus_tag="IWJ07_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAP9334086.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1996..2733
                     /gene="erm(B)"
                     /locus_tag="IWJ07_002564"
     CDS             1996..2733
                     /gene="erm(B)"
                     /locus_tag="IWJ07_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP9334087.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2996..3859)
                     /locus_tag="IWJ07_002565"
     CDS             complement(2996..3859)
                     /locus_tag="IWJ07_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HAP9334088.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3861..4133)
                     /locus_tag="IWJ07_002566"
     CDS             complement(3861..4133)
                     /locus_tag="IWJ07_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP9334089.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4150..4365)
                     /locus_tag="IWJ07_002567"
     CDS             complement(4150..4365)
                     /locus_tag="IWJ07_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HAP9334090.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(4457..4600)
                     /locus_tag="IWJ07_002568"
                     /pseudo
     CDS             complement(4457..4600)
                     /locus_tag="IWJ07_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
     gene            complement(4654..>4826)
                     /locus_tag="IWJ07_002569"
     CDS             complement(4654..>4826)
                     /locus_tag="IWJ07_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP9334091.1"
                     /translation="RPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSV
                     CTEIKVLLGIPA"
BASE COUNT         1476 a          819 c          892 g         1639 t
ORIGIN      
        1 atttcttggg agttttgaga ctactatatg cgtttttttg ttgatttacc aacatgttta
       61 gacattttcc tctaggaaaa aattaactag caccacgggt tatcttttat atagaagata
      121 tcgccgtatg taaggatttc agggggcaag gcataggcag cgcgcttatc aatatatcta
      181 tagaatgggc aaagcataaa aacttgcatg gactaatgct tgaaacccag gacaataacc
      241 ttatagcttg taaattctat cataattgtg gtttcaaaat cggctccgtc gatactatgt
      301 tatacgccaa ctttgaaaac aactttgaaa aagctgtttt ctggtattta aggttttaga
      361 atgcaaggaa cagtgaattg gagttcgtct tgttataatt agcttcttgg ggtatcttta
      421 aatactgtag aaaagaggaa ggaaataata aatggctaaa atgagaatat caccggaatt
      481 gaaaaaactg atcgaaaaat accgctgcgt aaaagatacg gaaggaatgt ctcctgctaa
      541 ggtatataag ctggtgggag aaaatgaaaa cctatattta aaaatgacgg acagccggta
      601 taaagggacc acctatgatg tggaacggga aaaggacatg atgctatggc tggaaggaaa
      661 gctgcctgtt ccaaaggtcc tgcactttga acggcatgat ggctggagca atctgctcat
      721 gagtgaggcc gatggcgtcc tttgctcgga agagtatgaa gatgaacaaa gccctgaaaa
      781 gattatcgag ctgtatgcgg agtgcatcag gctctttcac tccatcgaca tatcggattg
      841 tccctatacg aatagcttag acagccgctt agccgaattg gattacttac tgaataacga
      901 tctggccgat gtggattgcg aaaactggga agaagacact ccatttaaag atccgcgcga
      961 gctgtatgat tttttaaaga cggaaaagcc cgaagaggaa cttgtctttt cccacggcga
     1021 cctgggagac agcaacatct ttgtgaaaga tggcaaagta agtggcttta ttgatcttgg
     1081 gagaagcggc agggcggaca agtggtatga cattgccttc tgcgtccggt cgatcaggga
     1141 ggatatcggg gaagaacagt atgtcgagct attttttgac ttactgggga tcaagcctga
     1201 ttgggagaaa ataaaatatt atattttact ggatgaattg ttttagtacc tagatttaga
     1261 tgtctaaaaa gctttaacta caagcttttt agacatctaa tcttttctga agtacatccg
     1321 caactgtcca tactctgatg ttttatatct tttctaaaag ttcgctagat aggggtcccg
     1381 agcgcctacg aggaatttgt atcgataaga aatagattta aaaatttcgc tgttattttg
     1441 tacatttaac ttgacggtga catctctcta ttgtgagtta ttagtggtac agttttcaac
     1501 cgttttaatt ataaaaaagt ggtgcatttt taaattggca caaacaggta acggttattg
     1561 caggtgtatt tcttatctat gggtttaaca tggattttat cattaaaatc atgagtattg
     1621 tccgagagtg attggtcttg cgtatggtta accctaaagt tatggaaata agacttagaa
     1681 gcaaacttaa gagtgtgttg atagtgcatt atcttaaaat tttgtataat aggaattgaa
     1741 gttaaattag atgctaaaaa tttgtaatta agaaggaggg attcgtcatg ttggtattcc
     1801 aaatgcgtta tgtagataaa acatctactg ttttgaaaca gactaaaaac agtgattacg
     1861 cagataaata aatacgttag attaattcct accagtgact aatcttatga ctttttaaac
     1921 agataactaa aattacaaac aaatcgttta acttctgtat ttgtttatag atgtaatcac
     1981 ttcaggagtg ataacatgaa caaaaatata aaatattctc aaaacttttt aacgagtgaa
     2041 aaagtactca accaaataat aaaacaattg aatttaaaag aaaccgatac cgtttacgaa
     2101 attggaacag gtaaagggca tttaacgacg aaactggcta aaataagtaa acaggtaacg
     2161 tctattgaat tagacagtca tctattcaac ttatcgtcag aaaaattaaa actgaatact
     2221 cgtgtcactt taattcacca agatattcta cagtttcaat tccctaacaa acagaggtat
     2281 aaaattgttg ggaatattcc ttaccattta agcacacaaa ttattaaaaa agtggttttt
     2341 gaaagccgtg cgtctgacat ctatctgatt gttgaagaag gattctacaa gcgtaccttg
     2401 gatattcacc gaacactagg gttgctcttg cacactcaag tctcgattca gcaattgctt
     2461 aagctgccag cggaatgctt tcatcctaaa ccaaaagtaa acagtgtctt aataaaactt
     2521 acccgccata ccacagatgt tccagataaa tattggaagc tatatacgta ctttgtttca
     2581 aaatgggtca atcgagaata tcgtcaactg tttactaaaa atcagtttca tcaagcaatg
     2641 aaacacgcca aagtaaacaa tttaagtacc gttacttatg agcaagtatt gtctattttt
     2701 aatagttatc tattatttaa cgggaggaaa taattctatg agtcgctttt gtaaatttgg
     2761 aaagttacac gttactaaag ggaatgtaga taaattatta ggtatagggc acctctaata
     2821 actaccaatt aattcacctc aaaatgaaat tagaaaaaaa cagcgaaatt tttaaatcta
     2881 tttcttatcg atacaaattc ctcgtaggcg ctcgggaccc caacatgagg aaatgttttt
     2941 ataaaaaaag agtagttacc gttttttggt aactactctt ttcctttttt aaggtttaaa
     3001 tacctggaag tttaggtgtt ttgggtattg gtggtgtagg tggctgcaag ctttccaatt
     3061 tttgttgaat tgctttaaat tcaggtgtct cttggtgttt atttagaacc attttttgct
     3121 ctattcgttc taaagtcggt tgaatttctt tccctgatac tttacgattc aattcttttt
     3181 ctaaggtttc ttttggacta atagaaggtg tttccaaact tgaatagagt tttactcctt
     3241 ctctgttata tagccttata tcgctaaata agcccgtttt atgaagggtt tctaaattgg
     3301 tcggtaagtt tttgacaaca atatcatgcg cttgttttgg tgttgccctg gctgtcattg
     3361 gatcatctgc atacatggtt tcatatcgtt caattgttcc taaatatgag ttgattttag
     3421 gtactgccat gacatacatt tttgtttcat aacctttggc ttgaagcatt gttgcggttt
     3481 gaataggaac gtctgttgtt cgtcctgtac cttcgatcac caaattatac ccttgatcgc
     3541 tcaaacggct tatgatcgct tctgtcatgc gattagaata aggggtaacg tgttttacta
     3601 cgtctttttc ataaagtttc actagttcat caaaattagg gtgctgttgt ttaaaggtat
     3661 cattatcaat gacaataaca ttcccttgtg tttcttcaaa aattgctgat cgcaaactgg
     3721 ttttccctga ccctggttgc ccaccaagta aaaaagcggt tggcgattca accgcttttt
     3781 ttccttgaat caattcttct aaattatcat ttaagcgatt ctcaaattgt ttgtcagtaa
     3841 aattgactat atttgccata ttaagccact ttctctttat tcaaaacttg ttttgagtaa
     3901 cgattcattg accgccaata ctcatgaacg gcttgtaatt cttctaaaga ataatcaaaa
     3961 agatttacac gttttgcaag ctttaattgg tttaataaat tgacttccaa taattgagag
     4021 tcatttttat ttaattcatg gttcaaaaca tagtttaata ctcgattata aacgcttgcc
     4081 gataattcgt taatgatctc tatttcaaat gttttttcat acgtaactgc catttcattt
     4141 tcactccttt taaagtttgt caggtaaata tttccgtata tattcttcta aggcttgatc
     4201 cataacttct tttacgtttc cgccattctt tgctgtttcg atttttatga tatggtgcaa
     4261 gtcagcacga acacgaaccg tcttatctcc cattatatct ttttttgcac tgattggtgt
     4321 atcatttcgt tttgcttttt gtgcgcctaa atttcccaca atcactcact tctttctatt
     4381 tcttcttact cttattttat catcaacaat cacaaatcac aagtgatttg tgattaatca
     4441 cttgtgattt gtgattctat tctttttcgt tttctaattg aataattcgc tcaagcattt
     4501 caaaaaatac gttcttatac atggataaaa cttttttgtc atagccttta tgctctgtaa
     4561 tgccgttttt agaccaggta cttactttat tacttcgctg gttctgttgc aaagttttaa
     4621 ataaagaata aaatccctta cggtatctat gatttaagct gggattccca ataatacctt
     4681 gatttcagta cagaccgaaa acccgaagag agtgccttct tttcgggttt tcttatataa
     4741 tcctcgaatg gcttccatgc ctttaatcgt ggtagaggca gtgcgtaaac ttcgatagaa
     4801 tttattgcgt ctctttactg gacgat
//
LOCUS       DACDPS010000126         4726 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.126, whole genome shotgun sequence.
ACCESSION   DACDPS010000126 DACDPS010000000
VERSION     DACDPS010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.126"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..839
                     /locus_tag="IWJ07_002570"
     CDS             <1..839
                     /locus_tag="IWJ07_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HAP9334092.1"
                     /translation="YKALKYIEDAREKQLVSISINSPYARNYELEEIFKNYFKTNIYI
                     LKSSEDLSQHDLLFSKFAADYIQELIKKANVVALSWGDTVYKVIDQFKMGAREELIFT
                     QFIGEIGKYNSLAGSTRLVQKAAERYESSYLTLSIPLYVINETARELMALEPSLANTL
                     ATASHADMLISGIGTLSSIDSVEPWNKHRKILFPSMTDTVGFLYGRPFNINGEFIKAS
                     EDKTFGLSLKEIFDIPKRIGICKSKFKAESCLGALNGNFFTDLFLDEKTAWKILSLIK
                     KE"
     gene            complement(1136..1684)
                     /locus_tag="IWJ07_002571"
     CDS             complement(1136..1684)
                     /locus_tag="IWJ07_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334093.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1685..2542)
                     /locus_tag="IWJ07_002572"
     CDS             complement(1685..2542)
                     /locus_tag="IWJ07_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP9334094.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2828..3115)
                     /locus_tag="IWJ07_002573"
     CDS             complement(2828..3115)
                     /locus_tag="IWJ07_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP9334095.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            complement(3105..3434)
                     /locus_tag="IWJ07_002574"
     CDS             complement(3105..3434)
                     /locus_tag="IWJ07_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP9334096.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(3511..3783)
                     /locus_tag="IWJ07_002575"
     CDS             complement(3511..3783)
                     /locus_tag="IWJ07_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP9334097.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTYEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(3783..4046)
                     /locus_tag="IWJ07_002576"
     CDS             complement(3783..4046)
                     /locus_tag="IWJ07_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP9334098.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDLKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            complement(4152..4568)
                     /locus_tag="IWJ07_002577"
     CDS             complement(4152..4568)
                     /locus_tag="IWJ07_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334099.1"
                     /translation="MEFREGAFDFQTSQQNRKFIPEDFQNLQLENMRLEDLFRLADDD
                     KYRFEVLKIKKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNR
                     NSTDVVMITDIYVEDEVIIKYMKPVIKLVNKNKGNG"
     gene            complement(4574..>4726)
                     /locus_tag="IWJ07_002578"
     CDS             complement(4574..>4726)
                     /locus_tag="IWJ07_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP9334100.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFSFSVSTEIKV
                     LMGITA"
BASE COUNT         1402 a          899 c          655 g         1770 t
ORIGIN      
        1 gatataaagc tttaaaatat attgaagatg cacgtgaaaa acaattagtt agtatttcaa
       61 ttaattctcc gtatgctagg aattatgagc tagaagaaat cttcaaaaat tactttaaaa
      121 cgaatatcta tatattaaaa agtagtgaag acttatcaca acacgattta ttattttcaa
      181 aatttgctgc cgattacatt caagaattaa ttaagaaagc caatgtggtt gcactatctt
      241 ggggagatac cgtttacaaa gtgattgatc agtttaaaat gggtgctcgt gaagaattaa
      301 tttttactca attcattggc gaaatcggaa aatataattc cttagctggc tccacacgat
      361 tagttcaaaa agctgctgaa cgttatgagt cttcttattt gactctcagt attcctttat
      421 atgtcatcaa tgaaactgca cgtgaactaa tggctctaga accttcttta gctaatacct
      481 tagcaaccgc ttcgcatgca gatatgttaa tttctggaat tggcacctta tcctctattg
      541 attctgtcga accctggaac aagcatcgta aaatcctatt tccaagcatg acggatactg
      601 ttggatttct ttacggaaga cccttcaata tcaatggtga attcatcaaa gcatctgagg
      661 ataagacttt tggtctttct ttaaaagaaa ttttcgatat tccaaaacga attggtattt
      721 gtaaaagtaa gttcaaagcg gagagttgtt taggcgcctt gaatggtaat ttttttacag
      781 atttattttt ggatgaaaaa actgcttgga aaatactttc tttgataaaa aaagagtgag
      841 tcatgtaaaa tcctccaaaa aatttagatt tttggtacta cttttttgga gggcagtaca
      901 atgattcact ctttttattg gatatcaaaa tccctttttt tgagaagctt gtcttgatat
      961 tttttgaact tttccaagcc atactccatt acttttcaag atttaatttc tttgtattca
     1021 ctgaataatt tccacgtttt ttcgttaatg ttacttctta ttaattttat tgtacttctc
     1081 atgcaatagg atcatatatc cttctaaatc ttttttatgc tataaaaagg gggttctatc
     1141 taactttatt attcattccc caccctttgc gcagtgagtc ttcggtatct tgttttatct
     1201 gactggtagt tcttattatt tctccacggt catctaattc tggaacaatt ggatattttt
     1261 ctctgaacaa gtctagttct ttcaccacgt cattaaacaa atcttgctta tcttcttgcg
     1321 taagattctg tttaatatac tcatctaaag cttttctaag caattcatct cttgtcatat
     1381 tagtctcata cttttctact aaaactctgg aagtcgcatt taacaaagtc gacagtttta
     1441 aaggatgctt ggtggaataa accttatcaa actttctgat tggtgcgcca actttttttg
     1501 ttcttcctct actctgtctt tcaacattgg aggaagtttt tatttcttga acttggtgca
     1561 cttctaattg cttattttca gatggtgtaa ttgcttgctg ctttttcttt gcttctgctt
     1621 ctttttttat tttggcgagt cctttgttgt tcccaaaaat atcaagtcct ttgatgtcag
     1681 ccaattactc cacctccgca taccaattct cgtgttcttc caattctttc aaaatactga
     1741 taaataattc gtggcttttg cgatcccatt gttttaagag acctttcttt gtcttctcca
     1801 tcgtaatacc tgatccgtcg tatcgtttta agcgcgcatt tttgagtaca acatttttaa
     1861 tcaaatgttc cccataaatc tcttgggctt gtttatacat ttcctgatct actgcgtcac
     1921 cgctttccaa catgaaagga ataacaccag caacatataa atttgtatca aattctttca
     1981 caaaaaagtc catatactca agatattgca tcgcatttct catcgatttt acttgggtct
     2041 gtaagacgac gattgcccag tccgcaatgt acatcgcgga tttactatat acagaaatag
     2101 aaggtgggac atctaaatag atgacgtcaa actcatcaaa taatggttct agcttttctt
     2161 tcatatattt gaattgggat aatgaatcat tatattggtc tttgatccaa tcatcataca
     2221 tttcaaagga catatctgct ggaagtatat agaggttatt gattacttta tagacagcct
     2281 ctttaggtgg aagattgttt atcaacacgt cagtcacagt attttcgtaa acaatcggtt
     2341 caccttctcc gcctccttct tcgttagtga agttcatatg cgttaagttc atcacatcag
     2401 aagtatttcc ttgcatatcc aagtcaatta tcaaggttct ttttccttca ttaactgctg
     2461 attcaaaccc taacatttgc gcgcacgtac ttttacctac gccaccttta aaattaccaa
     2521 ttacatattt cttcgctacc acgttcctac ctcttttctt ttagcaatat tcatttgata
     2581 ctattataac atatttattt ttagttacta ccaaaaaaaa gatatatatg tactacctta
     2641 taattaataa gtaaaaggta gtacataaat aaggaaaagt tgaaatatat tgatttatag
     2701 ctttcctcaa agtaatgacg ttatttaatt tatcgagaag cagagaatac tcgcgacttt
     2761 agtcatgtag acgaatcaga ttttcgataa aaaagaagcc gtccttctta agaacgacaa
     2821 caaatcacta gattaatttt ttcaactgtt cataaaagtt ttcatgcgtt ccacataaca
     2881 atactggaat aaccttgttt tctataatct cgtaagctac tctatatgtt gtgcccgcat
     2941 atttgaaccc actagaccaa atacctgata aatccccagt tttttgttca cccttatatg
     3001 gatcaatcgc aatttcatca taaattaaat tcaaaaattt ttctttcaat ggcttctctt
     3061 ttagcttttt taaatactta atgacatgtt ttgaaggaac gatttcataa gccaatttct
     3121 gttgccaact cctctttagt cattacttta gaattagtaa gtgtatcttc agcaatatct
     3181 ctaaaggaag aatgcatttt gttttttcta ttcttaaatt ctacaagaat ctcatcttta
     3241 tcgtagcctt ctgcaattat atcagacaaa atatcttcgc taaaatcata aaattctttt
     3301 tgtggttcaa caaattcata aagaatacca ttctctacca actttgcttc aacttcgact
     3361 ccattaggaa cattaaaatc tttaggtact gtcaacatga ttgaattgcc ttgttttctt
     3421 gtttttgtaa tcatttaaat ccctcatttc taacagtatg taattactta gtaaatacat
     3481 tataccacga gataatgtat ttacaagtaa ttactgtgac tcgtaatgcg tccttgctga
     3541 ataaatttca accacgtgtt catcttcatc taccttataa acaacccggt gttgtctatt
     3601 caatcttcta gaatacctac cttcatatgt aggttttaac ttctcaaatg attgggtagg
     3661 gagataggga tcttccttta aagtctgaat aaccttctca aattgaggtt tcaaattaga
     3721 ctgtttgatt ttctttaaat cagctttagc cgagttttta atcatcacag aatagttatt
     3781 cattataagt tatcccaatc aatttcatca acattagtaa atccactatc atctttttct
     3841 cgcttacgaa caacacccaa agtgcctatt tgctcaagta atagcgtttc tttgattgct
     3901 ttcaaatcat ccaaaccaat aagcaccgca ttattttcac tgcgctcact aatgatttca
     3961 atttccgtat gattctcatt tacatctttc aacaacttat aaaaattttt ccgtgctgag
     4021 ctaggattta agactgtttt tgccatatct actccccctc taatttatat ctccattcta
     4081 acgtacttaa ttaagtacgt aaattcgatt gttttgttta aattggagta ccaaaacagc
     4141 attgtaaagt gttatccgtt tcctttattt ttattaacaa gttttatcac aggcttcata
     4201 tatttgatta taacttcatc ttctacgtag atatctgtta tcattacgac atctgtacta
     4261 tttcgattga gtactttcac ttgatctcca gcattgattt tccctctaaa tcttttagga
     4321 atgaaccaac tatacatatg attagcatca ccaaaatgtg cttttatcgc attctcagga
     4381 tataacttat tcaatagttc attcttttta attttcaaaa cttcaaatct atacttatca
     4441 tcatctgcta atctaaataa atcttctaaa cgcatgtttt ctaactgtaa gttttgaaaa
     4501 tcttccggta taaattttcg gttctgttgc gaagtttgaa aatcaaacgc gccctctctg
     4561 aactccaaat atcttaggct gttattccca ttaatacctt gatttcagta gacaccgaaa
     4621 agctgaagag cgttccattt cttcggttct ttttatatat tcctcgaatg gtctccatgc
     4681 ccttaatcgt ggaagaggct gtacggagac tttgataaaa tttatt
//
LOCUS       DACDPS010000127         4679 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.127, whole genome shotgun sequence.
ACCESSION   DACDPS010000127 DACDPS010000000
VERSION     DACDPS010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.127"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(301..912)
                     /locus_tag="IWJ07_002579"
     CDS             complement(301..912)
                     /locus_tag="IWJ07_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9334101.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(1035..1160)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(1242..1958)
                     /locus_tag="IWJ07_002580"
     CDS             complement(1242..1958)
                     /locus_tag="IWJ07_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP9334102.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(1964..2533)
                     /gene="scpB"
                     /locus_tag="IWJ07_002581"
     CDS             complement(1964..2533)
                     /gene="scpB"
                     /locus_tag="IWJ07_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAP9334103.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(2517..3314)
                     /locus_tag="IWJ07_002582"
     CDS             complement(2517..3314)
                     /locus_tag="IWJ07_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAP9334104.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(3284..3655)
                     /locus_tag="IWJ07_002583"
     CDS             complement(3284..3655)
                     /locus_tag="IWJ07_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP9334105.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(3674..4561)
                     /gene="xerD"
                     /locus_tag="IWJ07_002584"
     CDS             complement(3674..4561)
                     /gene="xerD"
                     /locus_tag="IWJ07_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAP9334106.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         1284 a         1009 c          798 g         1588 t
ORIGIN      
        1 aaaataataa cacgcatttt gtaaaacttt tgttcaaaaa gttcgttttt ccctattccc
       61 tcctcttttt tcagtttttt aaagctttct tctttttcat tgtagcgttt cctttttttt
      121 ataaacaaac attttcgtct tttaaaagga gaagaatttc acatttgcaa agaattgatt
      181 gatttttatg cataaatcaa aaaaatactc aaaacaaaca aataaaacgt ttacattatt
      241 gcatgtcttg tatttttttc caaaaaaaaa gaccaagaaa tttcttgatc tttttgatat
      301 ttatttgact gctgtatgac gttgttcgaa ctgatgttgt tttcgactta accatggaag
      361 aagttgcgca tgcagcacta aaaagacagc actcacgatg aatcctttga tcaagttgaa
      421 aggaacgatc ccaatcagta catagtttgc taaagacatg cccagcatct gtgttgcgtt
      481 cagccctaaa aacattaagt aaagaggcgt aataacgaaa taatttgcga tactcataaa
      541 aatagttaga gcgatggttc ctgtaatgac acctaaaaat ttgtttcttc ctttatgctg
      601 tttctttaca ctaaagaaat aataaatcgg gaatgtaaag atcgttgttg ataagaaact
      661 agcagtgtct cctaccaaat tatccggaga aaaacctgta gtcagcagat gtaatactga
      721 acgcaagaac gctgttgcaa ctccggccgc tggaccgaat aagaacatgc tgatcaatac
      781 cgagatatca ctaaaatcga tcttcaaaaa actaaaagcc ggcatgatcg gaaaagcaaa
      841 atactgtaaa actaatgcga tcgcagcaaa catcgctact gcaaccattt tctgtacacg
      901 ggtgttcttc atttgaaatc ctcctgttag gactcctctt cagcgaactt tttgacgttt
      961 ctctccaaat gaaaaaactc tattttccag ggaaaataga gttagagtta tttgatcaag
     1021 cgtcgtcaaa taagctctat cttctttatc cagactatac tgtcggtatc ggaatttcac
     1081 cgattcagct atttttttgc aaaataggtc gtggactata accaccggtc gggaatttca
     1141 ccctgcccct gaagacgaac cttatataat ttttgtttac atattcatta tacatatcta
     1201 ttcaaaaaac gcaacatcta acaattataa aaagtaagtg ttcattttga gtggttcagc
     1261 aaactgctga cttctttttt gttcagttca cggtaatccc caggacgtag gccttgtaaa
     1321 gtcagatctc cgtatttttc acgttttagt ttttgcacag gaagaccaac tgcttgaagc
     1381 atatttttca cttgatgatt acgtccttcg tggatcgtca attccacgat actgtggttt
     1441 gtcttaggat ccactgataa aatttggaat ttagctggag atgtttttcg tccttcgatc
     1501 tttattcctt ttgtcaaagg tgcaagcatc gattttgtag cgatcccttt tacctttgca
     1561 acgtaaactt tatccacttc atgttttgga tgtgtcaggc gctgagcaaa atctccatca
     1621 tttgtcagta gcaagatccc cgaagtatcg taatccagtc gacctacagg atagatacgt
     1681 tcttttacca tcggcaaaag atccgtaaca acttttcttc ctttgtcatc tgcaacagca
     1741 gaaataactc ctttaggttt gtacaataaa tagtaaccgt attcttcttg ataaattggg
     1801 acttcatcca cttccacagt gtcatgtttc ccaactttta tccctaattc ccttacaact
     1861 tgtccgttta cttttacacg tcctgcaagg atgaattctt ctgcttttcg acgagaagtg
     1921 atgccagcgt gggcaattac tttctgcaat ctctccatta caattattct cctttattca
     1981 ttccatcaaa gaatagatct ggtgacacgt cttcttcaag tgaaatttcc atttcttgtg
     2041 tatcaggcag ttcttctaat gatttcaaac caaaataatc taagaagtag ccagttgttc
     2101 catataatat cggccgacct ggtccttcta ctctaccttt ttcttcaatc agtttatggg
     2161 cagctagacg ctgtaccgct cctgaaacct gaactcctcg aatctgctcg atttcagctc
     2221 ttgtgattgg ctgtttataa gcgatgattg ctaaggtttc gacggcggct tgagataatg
     2281 tgtttgatgt cccttgcgca tatcgcttaa gtaagggagc cagctctttt ttagtcgtta
     2341 atacataatg gctttccgtt tcaaaaatat gcaaagctga gtgcgagttt tgagtgtaag
     2401 attcttttaa ttctttcact aaacttgtga cttctgcttc gctgagattt aacagtccgc
     2461 ttaattcttc attactgatc ccttcctcgc caacaataaa aagaagggct tcaagttcac
     2521 ttaatttcgt catgttcggt atctcctact gctggataaa gcataattga ttcatagttt
     2581 tctttttgtt gcgcaatgat ctgccctttt ttcatcaatt ctagcaaagc cataaaagta
     2641 gtgacaactt cacttttaga aggtgactca aaaaaagaat cgaaagtaca gccatgcgca
     2701 tttcttttta tacgctcttc gataaatacc attcgttctt cgatcgttgt gtcatcgggc
     2761 gtgatcgttt tttccacttt ttgagaaaat ttcttacgtt ctaagatttt ctgcatggca
     2821 cggatcaaat cgattttttt gacttgtcct cgcttcaacg gcttttcagc atccgctaac
     2881 ccatcgatgt tcataggttc ttttgtgtaa taaagacttc gttcctctgc tttttctgtc
     2941 agtctttcag ctgcatattt atatttacgg tattctaaaa gctgagtgac caattcttct
     3001 cgaggatctt cttcccataa ttcttcatcg tctgtgccga attcttggac aggaagcaat
     3061 gtcttgcttt tgattgccat caatgtagca gccatcacta agtattctcc tgcaacagct
     3121 agctcgagtg ttttcattgt atggatataa ttcatatatt gctccgtgat cgtagcgatc
     3181 ggtatatcat atatatcgat ttccatttgc tgaatcagat gaagcagcag atcgagagga
     3241 ccctcgaata catctaattt aatattgatc tccgtcaact cattcactct ttcttctttt
     3301 tctttgctag ccattttcca atgtaaggct ctgtgatttc agttgcacgg atggagacat
     3361 ccgggtactt ttcctgtaat tcatctaaca tttgaaagcc catattctca ccgcgataag
     3421 acggattcaa agaaatgtca tggatgatca gctgatcgtt cgttgcccag taaataccaa
     3481 taatcccttg aatgttggtt gaaccttctg gcgtaaagaa aaaaagttca taatcgtctt
     3541 cagattcgta cgtatcaatc tccttcaaca aagaagtttt acctgtcagt ttcttatgga
     3601 aactcaatag tcccatagca attttacggt tttctttccg gtaatgtgtt aacatcttct
     3661 caccttccta tgactaagcc cttggaaaat gctgcttata aacatccgtc attcttttct
     3721 tcgtgatatg tgtataaatc tgagtcgtag agatatccgc gtgacctaac aattcctgta
     3781 ctgtccgcaa atcagctcca ttttctagca gatgtgtggc aaagctatgc cgcagcgtat
     3841 gtggtgttac ttctttgtaa atcccagcct ctcgtactaa ctgcttcaga tttttccaga
     3901 ttccttgccg agaaagacct ttcccatgat tattgacaaa tacatgcatt tcagatgcgt
     3961 cctttacaag taatggacgt gcttcgtcta agtagcgttc cagccattgg atcgcataat
     4021 cacctaaagg aatgatccgc tctttatctc cttttccaac tgtctgtacc agtccaagag
     4081 aaagatgaag atctcccaat ttcaatccaa tcaattcact gacgcgcatc ccagtagcat
     4141 acatgacttc caagattgca cgatctctta tccctaacgt ttttgtcgta tcaggtgctt
     4201 caatcagctt ttctacttct gtcagagtca atgtacttgg taatttctgt gctttcttcg
     4261 gcgtatcgat atgttgcatc ggatcatgat cagtgatacg ctcttgccgt aaaaactgat
     4321 ggaatcttct gagacttgag atcatccgag taacagttgc tgtcgctttt ttttcttcat
     4381 gcaacttctc cagaaaacgg atgactaaaa agcgatcgac atcctgccat gaagcaattt
     4441 tctgctcctg tagaaaatgc aagtactgtt ccaaatcacg ttcatagctt tgtcgtgtat
     4501 tggcggacaa tccccgttca atattcaagt agtggagata atcgttgatt tgctctttca
     4561 taataatccc cctcatgcct ctctatcata tcagataccg cttcattctg atagtctttt
     4621 tccataatct gagcaaagaa aaagccgtct agacactcag acggactggt ttctttttt
//
LOCUS       DACDPS010000128         4643 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.128, whole genome shotgun sequence.
ACCESSION   DACDPS010000128 DACDPS010000000
VERSION     DACDPS010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.128"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            247..1388
                     /locus_tag="IWJ07_002585"
                     /pseudo
     CDS             247..1388
                     /locus_tag="IWJ07_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(1514..2593)
                     /locus_tag="IWJ07_002586"
     CDS             complement(1514..2593)
                     /locus_tag="IWJ07_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9334107.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(2696..3136)
                     /locus_tag="IWJ07_002587"
     CDS             complement(2696..3136)
                     /locus_tag="IWJ07_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9334108.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(3193..3603)
                     /locus_tag="IWJ07_002588"
     CDS             complement(3193..3603)
                     /locus_tag="IWJ07_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP9334109.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(3783..4217)
                     /locus_tag="IWJ07_002589"
     CDS             complement(3783..4217)
                     /locus_tag="IWJ07_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP9334110.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(4317..>4643)
                     /locus_tag="IWJ07_002590"
     CDS             complement(4317..>4643)
                     /locus_tag="IWJ07_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9334111.1"
                     /translation="ILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
BASE COUNT         1679 a          883 c          783 g         1298 t
ORIGIN      
        1 aataaagaaa cgatgttcag ataggaaaag aaaaatatct aattttgtat tgcttttttt
       61 cttcaagata aacgttttaa tcgcttcttg agtttgcatg aaagaggctc ctttttactt
      121 aaattgttaa aacttgaaaa tattactgtt tttttcataa atttagacaa aaaaacagta
      181 aacatatagg tagaatctct actatcttat ttcttgcata caaaaaaatc aaggagtgag
      241 agagttatga gatattcaac catcttaata tcaacatttg cagccttaag cttatcgctt
      301 actataaatc agacagtcca cacagaggag acagaaacag ccaaatggac agaaaccaag
      361 tgggccaacg tcaatcgaat tacctttcag gatgaatttt catccttcaa ttcaggagtc
      421 gcaaaattaa gaaaaggaca aacagcgacc atcaaagcaa aaattgacta ttcgggagac
      481 ttagcaccta tactacaatc agctgttaca tttgagaatg ttgatcccac tttgtcgatt
      541 ggctatgacg agaacacttt gactatccaa cacaagcaag cgatatttac cttcaccgtt
      601 acattaaatc aggacttgtt aaaaccagcg ctttttactg tcaaggtatc agatggacta
      661 ccgaacgatc atcatttaac gccgtatagt cagcggcaga caattgagca agattcagcc
      721 atcgactcag gaggagatct tgttgaagaa ccaacagata aaccagaaaa tgaaaataag
      781 ccagaagtac cgcctacaga aaatccagac ggagaacaga aaccggaaat agaacctgga
      841 gaagaacccg atacagaaac tcaaccagag ccagacaata aatcaaaacc tgaaataacc
      901 cctggagaaa aaccagatgt aactccagaa ccagatacgg attctggaaa ccaaacagta
      961 ccggaaacaa atccagacac agataatgaa actgaaaatc cagaaaaacc agaggtagat
     1021 ccagaagaaa aaccagatgt gactccagaa ccagatacgg acgcaagaga ccaaggaata
     1081 ccagaaaaaa taaacaaaaa aacaattcaa gaggatggaa aaaaagagag taaaaaatca
     1141 aatctagcaa ttctaaaaat aaatgaagag cagctgaata aaaaatcacg aatatttgat
     1201 agcgcccaat cagctgaaac cttaaaaagt agcaaggata caacctttgc tgtcctgaaa
     1261 cgaaaaataa acagctacca aaatccggag agtcacaaaa caaagttata ctgtggagcg
     1321 gaatcattct attaagtata gcaacaatgc tttctgcaaa aagatttaaa caaaacagat
     1381 ctctctaaca tttgaataat gtctgtgaac gcatttttca aataaaaaga aatatccaga
     1441 aaatgacccc aaaataccag attgaaatct gatatttggg gtcgttcata ctttatcttt
     1501 cttcatattt attttaaggg atatctgttt cttttggaag atggttcggg atctttttca
     1561 ctttcaatac atctaaaaag aaggtaaaga tcggaatgcc gacaatcaag ccccaaacgc
     1621 caaagaaccg ttcgctaaca agtaagacca caaatgtata aaaaatcggc agttctgttt
     1681 tgctggacat caacttcgga ttcagcacat aagactcaat tagatggaca atcaaaatcg
     1741 taatcaggat ataaacaaca tctcggatgc ctccttggga ataagctatg aaagatagag
     1801 gaatacaaga aatgataaca cctgctactg ggatcagact taaaatgaaa atcatgatcg
     1861 ctaaactcag cagctgatga aagccaaata cagccaaggc gattgtagtg agcaacgtat
     1921 ttaaaagagc gacaacaaat tgagcttcta ataccaaacc aaatgtattg acaaacttat
     1981 cggcaaaata gtaaatatct tgaaagaacc aagaaaattc actttttaaa aacaatcttg
     2041 agaatacaat tgtttttttc ttttcaatca taaaaaagaa acttaagatg aatgacatag
     2101 caaacgccac tgccattttt cctatatcct gtaaatagcc aagcgcaatc gaagcgccat
     2161 tttgaagctg ttcaaaaaaa tcgtttcgct caatataagt atggatatat tgtaaaacag
     2221 gatctgcatc ttttggctga tgctcgtaaa aattaataac ggactggatc atgtcaaatg
     2281 tttgatgaat aagcatcggg atatactttg tgaatgccag atagataagg taaacgatca
     2341 gtgtataaat catgatagct aaaacacctg tagggattcg gacaaatcgc tgaatgattt
     2401 gaacaagatg aatcatcaaa taagtaaaga taaatgtgag caaaatggtt gtaatcatac
     2461 ttctaactaa gaaaagaaca aaaacgacaa ataataaaac agtgagacgg cgtatttttt
     2521 cattcgccaa aaaacgctga taaacggtca aatcgacaac ctccaaataa caaaaaataa
     2581 acacgttatt cattatatga tggaatatga aaataagaaa gagcaaaaag aaaaaccgct
     2641 aaatgacgtt atcttatgag ttggttttcc tcataaaatc gttcatctag cagtttcagc
     2701 cctttttctg gtcttgatga aaattatcta gcatttttcg ggtcagatgt tgcagtgtat
     2761 ccaattcttc ctgttcaagt ggaagataag acttgatctt ttgtggaata ccttctgctt
     2821 gtttttgcaa cgcagcacct tgagcagtca gcgtaacatt tacgattctt tcatcacccg
     2881 tttcacgctg acgattaata taattttttt ctgctaactt ttttagtaag ggagtcagcg
     2941 taccagaatc cagacacaaa tcatttccta gctctttcac tgaagtcttt ttcttttccc
     3001 ataaaactaa catcacaaga tactgggtat aggtaagatt cagcggcttg aggaggggag
     3061 tgtagtgtct aatgatttct ttagaggcgg catataaagg aaaacatact tgattaacta
     3121 aatatagatc ttccattttt ccacctccgc tgttttacta tagtgtatca tagcggattt
     3181 acacaaaaaa gtctattcac taactgcttt cagagtaact tctatatttc cagctgtagc
     3241 ttttgaatac ggacacactt tatgcgctgc ctctaataat tcttgcgttt cttccagaga
     3301 aagaccttcg atagatcctt caatctctac acctaagaca aagctcgttt cactttctga
     3361 ataaagggaa acttgtgcac tgacagtaga agccccagag atattttttt gctgtttgat
     3421 caattctaat gcactgttaa aacatgcact gaaaccagct gcaaacaatt gttcagggtt
     3481 ggttgcattt tcgacgcgtt taccaggagc tgcaatattc aattcgaatg tacgatcggg
     3541 attatgcact tctccagagc gtccgcctgt gttgatcatt ttcgtagaat acattttttt
     3601 cataaaatta ccacctttaa ttatatttta tacaattgaa ttttacacaa tataattgtg
     3661 gaaagcaaag aaatggattg gcataagata aataagagaa agcacatgaa acagacgcgc
     3721 tttctcgaat agcaaacata tacaaaaaat atcagagaca aaaaataatc tcatcataaa
     3781 cattacatat atttctcaaa aagttgtttt ctttcttcaa aaattacttc acgatggacc
     3841 tgatgaagca tccataaata accaaaagga tctctgaaca ttgagttagt cacaccaaag
     3901 tccatcattt ctgtcatttc ttgaatctct acacagccat ttcgtaatgc tttattatgt
     3961 gtgtcctcga tatcagagac gatgatattg aaccagatcg tatttggatg ttcagcagta
     4021 ggagcagtca aaccaagaga cgtgttttca tccagcatat ggaatctcgt accatacaag
     4081 gtaaaaatga cttcattatt gcctctaggg taatctgttg cctcaatcag ttgtatttca
     4141 tcaaagattg tttgatacag ttctagtgct ttcaaactat catttaatac aaaatcgatt
     4201 tccgttcctt tatgcataaa gatctcctct ccttttcagt cgaacagtta ctagattctt
     4261 ttattatacc agctaaagaa aacgcattca aaaaaatagc ggatttactt accaactcat
     4321 tcgattggat gataagtaaa tccgcgtccc attacttctg ctacgctgct gacaatgaca
     4381 aaggcacttg gatcgatttc ttctatcaat ggtttcattc gggaaaattc atgatcagtc
     4441 acaacgatca ataaaacctc ttttgacttt cgattatacc cgccttttgc ctctagcagg
     4501 gtaaggccac gtccaatctc agcacttaag cgaaggcgga tgtcttccaa ataatgttca
     4561 ctcatgacca taacagattt ctttcgattg atacctgttt cgatataagt catgacaatt
     4621 gaagtgattg caatagataa aat
//
LOCUS       DACDPS010000129         4464 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.129, whole genome shotgun sequence.
ACCESSION   DACDPS010000129 DACDPS010000000
VERSION     DACDPS010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.129"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            574..1008
                     /locus_tag="IWJ07_002591"
     CDS             574..1008
                     /locus_tag="IWJ07_002591"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334112.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
     gene            2301..3224
                     /locus_tag="IWJ07_002592"
     CDS             2301..3224
                     /locus_tag="IWJ07_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9334113.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            3240..3779
                     /locus_tag="IWJ07_002593"
     CDS             3240..3779
                     /locus_tag="IWJ07_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP9334114.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
BASE COUNT         1664 a          668 c          758 g         1374 t
ORIGIN      
        1 aaaaaaaact gtgttggtac tgggttctat ttctctagga atattaggtt ttagccccat
       61 gctattatcc gtaattggaa aaaacaattc caacagagaa cttaaattga attcaaatta
      121 ttacaacagt cctaacgaag aattgtttgt aaaagaaacg gataaataat aaaaaagact
      181 gctcttccga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa gcaacgcgct
      241 tataaagcgc taccaaaaat ttatcatatt ttatgaaaaa taacaatcca gtaaaaaatg
      301 actggtaaaa attcacaaag aaacggaaag aagcaactgc agaaaatcaa gccttaaaaa
      361 gagcttttta cctagaccaa ttaaaaatca caaagtgagc ccctgtatcg gttgatacaa
      421 gggctacttg gcatactcga cattatgtct actaaccttt aaaataacac gttttataaa
      481 ataaaccctc ttgacaatat ttaattttct gattattcta aatatataca gaaagcgctt
      541 tcctaggatt ttatatctaa ggaggaagta acaatgaaaa ataaaattat taaaattatt
      601 acttgtctat ttttgttcgg tatgattgtt ggagcaagta ctccgttaaa tctgtttcct
      661 tttagtgcta gaggtataaa agtggaagct gcaagttcac gatataatca taatcatcgt
      721 ggatttactt gctataaaat gaactattat gttactaata aaatgtgtag agatttgaaa
      781 aaaaattata ataagctaaa gactcctgct caaattgcat cttttattcc tattggtgga
      841 gttggaacat gggttattac aaatgctttt agtagtgctg ttgatgcaat gaatgtcttt
      901 gtaagagctg caaatcaagg aaaaggtgta caattaacat ataatgctca ctttagcaat
      961 acaacatcat atcaatacaa tgattacgct cgttatgtaa taaaataatc agaaagtgaa
     1021 actatctcat gaataaaaag attttagctg taattatttc aataatagtt ttaatcacta
     1081 tgttcttcat atttaggcta attttcaata taagcttaca aaaaagtatt ctttatctaa
     1141 ttcctatagc atttatctta gcaattttta gaagtattta cggaaataaa aactctaaat
     1201 agctaaaaat aaatcatact aaattttaca aaaagtggct agcattttct agccactttt
     1261 tgtattgtgg agcgtgggtt ttgaggggga ttcaaggggg agcttgcgcc cctttgacaa
     1321 gcggttattt gcaccaaata cggaggtatt tggctagcaa atatagagct tgccaaaccc
     1381 ttttagtgag ttttgtagaa agtaccttta aaggtacttt cacattacga acgatacgtc
     1441 cgtaggacgt actgaaaggg tgggacttat gagcaaatta atgcaaaatt tggctagcaa
     1501 aaaagctaaa aaatatatgc taataatcga gcgaaataaa cactttttca agtgagcgaa
     1561 tccgccatga caaagctgaa gcagtttatt tcagctttgt catggcgtgt gccgactttt
     1621 ttaaaagtct aggcacacga gcaaatatct ttatcaagct ctaatttgcg ttttaagacg
     1681 tttgaaacgc ctaagtatat aaattatata agaataggtt taagagtgct taaatcgaat
     1741 tttagagggc atttagacaa ttttagggtt gttagggctt gccctgaccg cctgtaaggc
     1801 gttttaagaa caagataagg gatttttata ctaggactgt cttaaaatgt cttaaacgaa
     1861 cgctcagagc ttctcaaagg gttccttatg aacggctttt agcaaaggag ccggaggcga
     1921 ctgcgcagta taatgtgaga gcagagcgat cacacctttt tagcgaaccg tagggagcaa
     1981 ggtttggaat tggcagtttt gataaattcg cgaacctgtt tagagaaaaa aggagtgatt
     2041 ttttaggcgg gaaaattagc ctcccctctt tttatatctc tttttatatc tctttttata
     2101 tctcttttat gtcggctgtg tccttactct cccaagcaat tcagagtttt ttcaagtagg
     2161 ttttctacgc ttttcaagta ggttttctac gcttttcaag taggttttct acgcttttca
     2221 agtaggtttt ccgacattat tgagtatttt tataaagtat gttactttta tttcatactt
     2281 tataaaagta ggagaagctt atggcaaatg aaattgtaaa atatgaaaat agattaaatc
     2341 atattcctct gagaaaattt aatagtcgtg aaatgaactt atttttttca attgcttcac
     2401 gtgtacgtga aaaaggtgta gatgagatta ttttttcatt tcacgatttg aaaaaactaa
     2461 gcggttatac agaacgtggg gaacaattta taaaagatct tagtagcacg tatgacaaat
     2521 tattaagtat taatgcttgg aatgatgatg gaaatacttt aactaaattt gttgctttca
     2581 caaaatatag cattatacga gataaacaac ttgttataat ttcagttaat ccagaattta
     2641 aaggactttt taatcaatta aagaactgga caagatttaa tctagagcaa tttactaatt
     2701 tggatagcac atattcaaaa actatgtttc gattattaaa acaatatcga actcaaggct
     2761 ggactgaatt ttccaaagaa aaattttttg atttactaga tattcctaaa agttattgga
     2821 atagtccgtc aaatattgac aaatttatct taaggccaat taaagaagaa ctcactccct
     2881 tatttaaagg attaactatt cgtaaaaaat atggtaaagg tcgaggtaaa cctgttatcg
     2941 gttatcgttt tacttggaaa gctgaaatta accatgctga cgatttttcc aaagggaaac
     3001 aagaagattt acgtataaaa ttatttaata ttgatcacaa tggagaatta acacgagaag
     3061 aaaaatggcg cgctaaagat cgaatactaa atcttccctt aggcactcat gaagctgatt
     3121 ttaaacaaca acaacaaact gaagaaaaac aggcagaaaa acgagaactt tcaaacaaac
     3181 tcaaacaaga tttactagga aatttacaaa acttattcga ttaagcttaa ggagtattta
     3241 tggcagataa aacaatcaaa gaacttgcgg aagaattagg agttagtaaa acagcaatta
     3301 acaaaaaagt ttctgacaaa aatcgaaaac tttggtttgc aaaaaacgga aacaagtttg
     3361 tgattaacga aaccggtcaa aaagctataa aatcaatgtt tttagtcgat aacgcaaact
     3421 taaatcgaaa actagttggc gaaaaatcaa aaaccaaaaa ccataaaaac accaaccaag
     3481 tttccgaatt aaaactcctt tctttcctag aaaaacagtt aaatcgaaaa gatatacagc
     3541 tacaagaaaa agataatcaa ataaaacaaa tgcaaaaatt actcgaccag caacaacaac
     3601 tcactctaca agcgaaccaa caaattgaga acctacaaga acagctccat ttaacttata
     3661 cagaagaaac tccaacaacc gaatcaactg ccttttctga aaaaaaagca gacaatacac
     3721 aaccacttat tgaaaaaaag tggtggcaaa tttggaaatc taatagaacc aatctctaaa
     3781 attttctaga agttagttcg gatagtagag gaaaaagttg tactacctga tggggagatc
     3841 actcaaattt ttatgagaaa gaaaaaatag tttacaactg taaacaatca atgtttatag
     3901 ttgtaaacta tcaaactata tggtgactta ttgcttaaaa taggttgaaa tctcactcaa
     3961 tttttggtgg aaaaacaaaa taaattctaa tcgattttgt tgaaaaagtc ataaaaaaac
     4021 aaatgtgatt ttaacaaaat ttcaaaaaaa tcgtttgaaa tttcgtttat tttttttgag
     4081 aattttgatc atcgtagggt cattgtagaa tcattatgag atcatcgtgg gatcatcgaa
     4141 taaagtatgg gaaaggcttg cggtgcttgg tattaagggc attctccgcc tcccaggttc
     4201 ttgaccattt tctcttataa gtgggatttt taccacgttg agagaaaatg gtcaagaacc
     4261 tgggaggcgg agaaaacata ccaagcacca caagcaaaaa aagttagagt aacttccaaa
     4321 aaagaaaaat tttttagaac aacttttgaa aatttaattt tgatttatat ccgttcaatt
     4381 taaacatttg ataaactgaa ggctagtatt taaaatgtgt atacaagtgg tagcgttaaa
     4441 aaaagtggag gtataaccta tgtt
//
LOCUS       DACDPS010000130         4444 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.130, whole genome shotgun sequence.
ACCESSION   DACDPS010000130 DACDPS010000000
VERSION     DACDPS010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.130"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            153..1859
                     /locus_tag="IWJ07_002594"
     CDS             153..1859
                     /locus_tag="IWJ07_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAP9334115.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2017..2919)
                     /locus_tag="IWJ07_002595"
     CDS             complement(2017..2919)
                     /locus_tag="IWJ07_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9334116.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2916..3620)
                     /locus_tag="IWJ07_002596"
     CDS             complement(2916..3620)
                     /locus_tag="IWJ07_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAP9334117.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            complement(3651..4292)
                     /locus_tag="IWJ07_002597"
     CDS             complement(3651..4292)
                     /locus_tag="IWJ07_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9334118.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
BASE COUNT         1376 a          946 c          768 g         1354 t
ORIGIN      
        1 taaaaatttg gatttcaacg atagaaacac tgttttttat gtaattagtc caagattttt
       61 ctagactttt gctttactat tcggccaaat tggtttttct tctttttttc gttataatga
      121 atatacttat gaaaagaaat gaggaatgaa ccatgtcctt tgatggtgta tttacccacg
      181 cgatggtcaa cgaattgcgt gaaacgttac tatctggccg gatctcgaaa atccatcagc
      241 cttatgaaaa tgaaatcgtg ctggtgattc gttcacgcgg caaaaaccat cgattattat
      301 tatctgcgca cccaagttat gctcgtgtac agattaccca aatcgattat cagaatcctg
      361 ataatccgcc taacttcgtg atgatgcttc gaaaatattt ggatggcgct attttagaag
      421 acatcgaaca aatcgaaaat gatcgagtga tccatttcca ctttgctaaa cgaaacgagt
      481 tgggtgatct acaaaatatc atattgatcg ttgaattaat gggacgtcat agtacgatcg
      541 tcttagttaa tcgagaaact ggaaaaatac tggatgcgat caaacacatt ggaagctctc
      601 aaaacactta tcgttctttg cttcctggtg tagaatatgt tgctccgcca aaacaagagg
      661 tcttgaaccc atttagctcc gagaaagaaa aaatctttca gcggctttcc caaactgaac
      721 tagatccaaa agcaatccaa cgtcagtttc agggaatcgg tttcgatatc gctcaagagc
      781 tgaccaaaag attactggaa cgtcctaatg aaaagatggt tgtatgggac gaatttttct
      841 cagccatcag ccatcagcct attcctactt tttacgaaac ggaaaataaa gattttttca
      901 caccgattgc ttatcaggta ttgtccgaac aggcatctgc tgtaacagct tatccaacac
      961 tcagtcaatt attagatagc tattatcacg aaaaagcaga gaaagatcgg gcaaaacaac
     1021 aaggcggaga actgattcga aaaatcgaaa atgaactaaa acgaaataaa aataaattga
     1081 aaaaacgtga acaaacactc aaagaatccg aaaacgctga gaattaccgt agaaatggcg
     1141 aactacttac tacttttctg acacaagtac cacgcggcgc aaaagaagtt gttttaccta
     1201 attattatga agaagatcgg ccaatcaaaa tagcactaga tccggcattg acaccaaatc
     1261 aaaatgcaca gaaatatttc catcgatacc aaaagctgaa gaacgctgtt aagctgattg
     1321 gcgaacaaat ccaagaagcc aaagatgaaa tccaatatct tgaatctgtc ttatctcaac
     1381 tggaaattgc cggcccaatg gatatcgaag caattaaaga agaattgact tcagaaggat
     1441 acctgaaaaa gaagaactta aaaaaacaaa aacgtaagaa accatctcag cctgatcaat
     1501 acttttcttc tgacggaacg ctcattcttg ttggtaaaaa caatcttcaa aatgaccagc
     1561 tgacaatgaa gacagctaaa aaaaccgact attggcttca tgcaaaaaat atacccggtt
     1621 cgcacgtcat cattaaaagt gacaagccct cagatgaaac aattacagaa gctgccgagt
     1681 tagctgccta tttttcaaaa taccgatact ctgcccaagt gccggttgat cttgtacagg
     1741 taaagcatat ccgaaaacca aatggcgcca agcctggtta tgttatttat gaaaaccaaa
     1801 agacgattat cgtgacacca gaagaagaaa aaattacagc tatgaaacaa aacaggtaat
     1861 caatgagtgt ttctagaatc atatttgcgt actttcccac aacagcaatt agttataaat
     1921 tgtttgctac aaaaagagga gttgtgacaa aattctgtca caacccctct tttttgatca
     1981 acggtgttca ataaacagcg ttctcatctg cttttcttag ctctgctggt ccagtaaccg
     2041 agtaagctct ttgataaatc tttgagcgat ttcactgagc ggacgtttcg ccgaataaat
     2101 gatataaaat tcatttagtt cgccttctgc caaagggatt aaccgcaagt gctcgcgaat
     2161 ccctccaatc acaataccag atcctgaagc gtaagcatca gtttcaagaa cgatattcat
     2221 caaagtacct cgatcactcg tataaatcac tgtttcttga tcagggatct cgataaggtc
     2281 ttcataaaaa tacgggaaat tatttccatc ctgtgtaaaa cgaacttgag gataagataa
     2341 caattcttct ttggtgatct ctgcgtgttc agctaatgga tgtccttgcc ttaaaaaaat
     2401 atgcgttttg aattttccaa tgctgcggcc acttagctct ccttgttgta aatatcttct
     2461 gataccagct tgattatgct cattcatata aataatgccg atctcactgt gctgctcttt
     2521 gacttcttcg atcactctcg ctgttgtcgt ctccatgatc cgaaattgtt tgatctcgtc
     2581 tttgaattcc tcgagtagct gtgcggtcaa aggacctaag aaatcataat gctgggcagc
     2641 gatcgaaaaa ttgtctctct tttcactcgt tagatagtga gtttctaatg tatcaagctg
     2701 gaccaatatt ttctcggcac gatttaagaa gtcttgccct tcttctgtta aatatgcccc
     2761 tcgattcgtt cgagtaaaca gcgtgatccc taactcggtt tctaattctt tgataccagc
     2821 tgaaagactt ggctgggaga caaacatttt ctttgccgct tctctgaaac taccattgtt
     2881 tgctacagcg accacgtatt tcatctgctg aatattcatt gctcaaactc ctcgaaataa
     2941 taaatttctc ttccattcaa gggctggatt ggtcggttgt tcggtcgagc atactgacca
     3001 tcgaagggga gaagaaactc ttggttcagt tttcctacat gatccattac aaaccattgg
     3061 attggcccgc tggtaggcgg agttgtcaat gagccaatat aatggaaatg gcttttttgc
     3121 tccggaaaga acacatccgg atttacccaa tgctcatgtt tttctaagtt ccaactttcc
     3181 ccattttctt gattatacag ccaaccttcc tgtaccatta gctggtacat gacacccaca
     3241 actagattct cttttttgct gttcatatga acaaaatgaa attcaatatc ttcctgtttt
     3301 ccgttgatga tatgttcaga aggcaagtgg aaatgaatat ctgtcaaata atagcgttct
     3361 ttcttgaact ctacataact gagttggtca aaaggtacta aatgaatcgt gtttttgaat
     3421 tccttgtcag taaaccgttg cttttgataa tgaaaggaaa gcgtgtctcc ttctattttg
     3481 gtttcttccc ccttggtcaa atgaataggc gactgcaatg gaaattccgc acctctcgca
     3541 aaccaatcac ataaactcgc ccaattttcc ggacctttgt ctccagaata gccccattcg
     3601 acatccatat tctttttcaa ctatacttcc ccctgcatct catatcattt ttatttttct
     3661 tctttcttta caatcgttga gacggtttct aaaaacgcct cttttccata aaattcctgg
     3721 ttcaatagtt ccggaaatat ctctttaaag aaatactgaa cagaaggccg atgttcaaag
     3781 cttttaatcg tagtcactgc ctcaatgaac atctccttat aaactttttc cgggttattt
     3841 ttgatgattt catcaaaaga ttcataaaga aggtcaacct tatctgaaat ggctaatagt
     3901 tctccttcta cggtttcatc ttttccttcg aacaaccgac ggcgataaac ttcttggaac
     3961 tcagctggaa tctcttggtt gatgaattcg tccgtcatct tttcttcaac tgtttgcagc
     4021 atccctctta actctttgct tgcgtatttc accggcgttt tgatatcacc aataaaacgc
     4081 tctgtgtagt catgattcaa tgatttttca tagagcagtt tccaatcgat cggttgtcca
     4141 ttcgcttctt caatgtctcc tagaacttgc gcaatagatg ctacacgata cgaatgtgct
     4201 gcaactgtat gctctgtata tttaaaaaat ccaggtgccc ttgtgatcgt ctctaaattg
     4261 ttcaatccta aaataaattc attcaacccc ataaaagtta cctccctttg agatttcttt
     4321 ttccgaatat catatcatta aataactgat attcgcaaca tttccgctct ttaccatttt
     4381 tgaaacagct cccttaccaa aaaatagaga aaatctatat ataatagaaa aaagagaaga
     4441 gatt
//
LOCUS       DACDPS010000131         4343 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.131, whole genome shotgun sequence.
ACCESSION   DACDPS010000131 DACDPS010000000
VERSION     DACDPS010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.131"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..1300)
                     /locus_tag="IWJ07_002598"
     CDS             complement(<1..1300)
                     /locus_tag="IWJ07_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAP9334119.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKTNDWHKHIDFTKSDEN
                     IYLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     ILDDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDE
                     ISPHLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIE
                     RLMNDYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYIYRQREE
                     NERDKALMASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDI"
     gene            complement(1522..1884)
                     /locus_tag="IWJ07_002599"
     CDS             complement(1522..1884)
                     /locus_tag="IWJ07_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334120.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(1888..2196)
                     /locus_tag="IWJ07_002600"
     CDS             complement(1888..2196)
                     /locus_tag="IWJ07_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334121.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERMMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM
                     "
     gene            complement(2205..2624)
                     /locus_tag="IWJ07_002601"
     CDS             complement(2205..2624)
                     /locus_tag="IWJ07_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334122.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVI
                     MLDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(2617..2850)
                     /locus_tag="IWJ07_002602"
     CDS             complement(2617..2850)
                     /locus_tag="IWJ07_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334123.1"
                     /translation="MAEKQKHFGKKNWLVNLTGDNVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYVYGVAESNGEDND"
     gene            complement(2853..3821)
                     /locus_tag="IWJ07_002603"
     CDS             complement(2853..3821)
                     /locus_tag="IWJ07_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAP9334124.1"
                     /translation="MSKNGIIVNEWEKLTALSNRRLNVQNMYTPLKMQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQYEENIAYIEILKW
                     QDGKGRIDFNPNKIGHFLASSMKNFIHDLFLEPHFSRADIACDIIDVPDEFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRLYNKKLEQETKRKIVPPEIETWWRLELQ
                     LRRGKATDWHAMVHESLNSFASPHFLPLDVKTNDKVMIFGLIANPEMWGFLERRMKYK
                     LRDILKRESQNDELTNHLRETFSESADVLKKELDTWLQGLDVTEEE"
BASE COUNT         1226 a          978 c          615 g         1524 t
ORIGIN      
        1 ctatatcctt ttctgcaaga ccaaaacgga tagacaaacg agtgatcgat ttttgaaatg
       61 cgttttttaa tcgctctaaa cgctggcgta aagagcgatt ttcttctttc agttttttat
      121 tttccacttg taaatctgct aattgttttc taccagacaa atgacgctct tcttccaatg
      181 tagctacata ctcatttaat tctgtatttt gctcaagcat ctgtaaatat tgctcactca
      241 tgttatcagc atttctcttg gcatttgttg ctctcgtagc tcgttgatac aaatcaacaa
      301 aatcattccc agataaatga tacgaaccat caggaagcct cttgaaactt tctaaaggaa
      361 ccatatcttt ttggaaaatg accgccatct ctttttttac attcagcata tttttcttct
      421 cttcttcaat cttcgctctc tcagaagcca tcagagcctt atcacgctcg ttttcttctc
      481 tttgcctata aatatacctc aactcatcag aaacgctctc acgctctttt acagccgttt
      541 ggcgcaattc agaaatttca ctcatgattt ctttgtattc gtgaatatct ttaattttgt
      601 tggtaccgac aatatccctt ttccaaccgt aatcattcat taatcgttct atagagtcaa
      661 cttcttgatt tctaaaatta ttgaaaagca ctcgaccatt atctttatca gcgtgtatcc
      721 cttgttgaac cagtgcttta ttaaagctcg gctgcttttg aacaccccgt ttataacctt
      781 cagcaaccgg cacaatattc aagtgcaggt gcggacttat ttcgtcatta tgaataacag
      841 cattataaac agccaaatta gggtttctct cttgaaactc cttgtaatac tgaaccaaaa
      901 ttttattggc aatcactcga ttttgttcag cttgttgttt cgatatgcca gtagaagaac
      961 catctgctcg atttgtctgg aaatcatcta aaatgcctat ttgcacaata aattctcgtt
     1021 gaggttccaa ctttttatcc ttgcgcactt tcgctaaata gtcctcgatt tttctatcgg
     1081 ctcttttttg tttcgcatta tatttttcta ccacttcgcc aaacaattcc tcatatttat
     1141 ctgctagatt cgtctgctct aaataaatat tttcatccga tttcgtgaag tcaatatgct
     1201 tatgccaatc attggttttc tggctatcag ttaactcacg attattatga gaaatacttg
     1261 tattcttcgt tcctttttta aatgaaatac taaaccccaa gttaaaaccc tccttcgcgc
     1321 tgtcgctaat aaaaagagtg taaacctttc tgttactctt tgtcaaagag taacattttc
     1381 ctaattgtga catggctgtc acaattccaa atgctctccg agggtattcg ctgtcacaag
     1441 tgacaacgaa aaaaggagcc gaaagcgtgt cacgctctca actcctttta cgctttattt
     1501 tgcttcacgc aaaataatcg cttatcgtaa attcacgtca ttaaatattg acgtaaattt
     1561 actctcactc atctgacttt tcttattcgg tatcactccg aataagccgt cagattcatc
     1621 gggataatct cgttcgcttg gcgctgattt ttcatcagca agccactcat tatccactga
     1681 tttttcgtgt tgctgatcgt cagaaatcga cgatagccac cgctcaaaat cagcaaaagt
     1741 ttcaaaccca aaacgcttgc gaagagcctt gcccattgca gcattttctt tctcaatcaa
     1801 gcgttttttc tgttggcgaa gtttcgcttg ttcttttttt aatttttctt cttgcgcttt
     1861 aatatctgct aaagtcattc ccataaacta catctccttt tctttggaat taataacgga
     1921 aatttggttt aactgcataa catgactcat taatttcaac caaaattctt tttctgattc
     1981 atcagttgtt tgcgctaaat gcttatgaac aaattcatta gtcaaaactg aagattttgc
     2041 tagatcagca ttgataaccg agttgagaac atccatcatt cgttcaagca tttcactata
     2101 attatcttca tccggcaaat tcaaataatc agctaaccgt tcatacatca attcaattct
     2161 atcgactgtt tcttctctca aatcaatttt atccatgtca cacctcactt ccagaattgc
     2221 caccattttc gattttttaa ttcttgattt tctagtaaca actgatcgtt tttttgttgc
     2281 tgaagcaaag tcaatttttg ttggtcacga atcatttccg ttaattcaga aatatgccta
     2341 tctttttccg ctaattgttc acgcaaaaat tcattttctt tttctaacgg gaaatcattg
     2401 cctgcatcat tgaccggtaa atcaccagtc aattgaccac cttttgaccg ataaattgac
     2461 cgcacaacac caatcaattc cttgtctaac atgataaccc cattatcttt ataccgagca
     2521 aggtcataac ctgcattgac cagttgattg cgcactgtat ttttagatat acccaactca
     2581 tcagcaaggt tttttatggt cattttttct ggcaaatcag tcattatctt ctccgttact
     2641 ttcagctacc ccataaacat atttaacagc atgcttaggg ataatgattg gggaatcaat
     2701 tttattattt ttcaaaatca actcatactg cgttacatca agtaacttac cggacatttt
     2761 tttgctatcc aaaaaaacaa cgacaacgtt atcacctgtt aaattcacca accaattttt
     2821 cttaccaaaa tgcttttgtt tttctgccat tcttattcct cctccgttac atctaaccct
     2881 tgaagccaag tgtctaattc ttttttcaaa acatctgctg attccgaaaa tgtttctcgt
     2941 aaatgattcg tcaactcatc attttgactt tctcttttca aaatatctct caatttgtat
     3001 ttcattctac gctctaaaaa cccccacatt tcagggttag caatcagtcc aaaaatcatc
     3061 accttatcat ttgtttttac atcaagtggt agaaaatgag ggctagcaaa actatttaaa
     3121 ctttcatgaa ccattgcatg ccaatctgtt gctttcccac gccttaactg caattccagt
     3181 cgccaccaag tttctatttc tggtggcaca attttacgct tggtttcctg ttccaatttt
     3241 ttgttgtata gccgaatctg gcgttcgctg gcacgactgc cccaataagc tgtttccagc
     3301 ttcccagacc gcccataaat tggcttgaaa gaaacaggat caaccactcg atattgtgta
     3361 ataaactcat caggcacatc gataatatcg caagcaatat ccgcacgtga gaaatgtggt
     3421 tctagaaata aatcatgaat gaaatttttc attgaactag ctaaaaaatg accaatctta
     3481 ttcgggttaa aatcaatccg ccctttgcca tcttgccatt tcaaaatctc aatataagca
     3541 atattttctt cgtattggtc taataattgc cagccgttat tcccaaccgc ttttaaatag
     3601 ccattgcctt cattcacccg cattaattgc tcaaaatcta aaatcaacac atcattttga
     3661 gtgtgataat aaatattttc cttcagtttt ccaacgatag taattcggtc aattgaccac
     3721 tgcattttta agggggtgta catattttgt acatttaacc gcctgttaga tagggctgtt
     3781 aatttttccc actcatttac gattatgcca tttttactca aattttgttc ctcctcaaag
     3841 aaaaagagac ttctggaaag aattttcaaa agttcacgta actcttaaaa attcattacc
     3901 aaaaaatctc tagtcttgaa gaaatctaaa aatattcgta taatgattaa gaagttacgg
     3961 agctagtgcg attagtttcc gtaaaggtcg agaacgttgg tagcgttgct cgactttatt
     4021 tttttgctct tttttcagtt tatttatttg ataactcaat aatagcactt ctctttagct
     4081 atgcaaatat ccaaaaacat aaaaccgcac cctttgaaaa gggcgccccc aaacttcgct
     4141 aacgctaggt tggccaaaaa atggactggc cattttttag caaatgtcag cttaaaaggc
     4201 tagtggttta gccgtcaact ccttatcctc ggcacaaacc gcctccggta ccgttgactg
     4261 cacatcgtaa ctaccactcg atctcatctt cagaacgctc taatttatgc tgtgagacgt
     4321 tttgacgata aaataaggtt ttc
//
LOCUS       DACDPS010000132         4334 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.132, whole genome shotgun sequence.
ACCESSION   DACDPS010000132 DACDPS010000000
VERSION     DACDPS010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.132"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            381..1688
                     /locus_tag="IWJ07_002604"
     CDS             381..1688
                     /locus_tag="IWJ07_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAP9334125.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            1807..3006
                     /locus_tag="IWJ07_002605"
     CDS             1807..3006
                     /locus_tag="IWJ07_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP9334126.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(3033..4301)
                     /locus_tag="IWJ07_002606"
     CDS             complement(3033..4301)
                     /locus_tag="IWJ07_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAP9334127.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
BASE COUNT         1360 a          904 c          805 g         1265 t
ORIGIN      
        1 aaataaaacg gtttctacgg ttttaaattt agctattttt ttaagttatc ttcataattt
       61 cctcaattat ttagccccag tcttcaacta ttcttcatga aatgctgata aactaagaaa
      121 aagaataaaa gaggtggatc ttatgcattt gattatgatt ttcagtttat taagtattat
      181 cagtttattt gctttcagcg cttcccgctt attagatgcg gaaacacagc ctattcctgt
      241 aaaagtacgt aaaaacgacc gctaaacttc cataacgcgc gttcttatcc taaagttttt
      301 tcagaaacat tgaaactctc tcccctctct gataaaattt taatttgtag cgaaacaaat
      361 taattacaag gagatcggat atgtccataa aaaaaacacg tctagtccta ttattggcta
      421 gtctcattct ttgccttgct tcttttctac cagctgttgc tgtgcaagct gaagatacgt
      481 tcaaagtaaa tgcaaaagca gcttttgctg tagatgcgca aagcggtaag attctttatg
      541 atcaagatgg agaaaaaacc atggggatcg cttcaatcac aaaaatcatc ggtctttaca
      601 tcgtccttga tcaagtaaaa gaaggaaaac tgtcatgggg tgataaagtt tcgatttcag
      661 attatgcaga aaatttgagt atcaccccag atttatcgaa tgttcctttg cataaagaaa
      721 acacctacac tgtcaaagaa ttattcgact ctgcaatcat ccagtcagcc aatgcttcaa
      781 tggtggcctt ggcagaaaaa atctctggca gcgaagctaa attcactgaa cgaatgaaag
      841 aacaattgaa agattgggga atcaaagatg caacaatcgt taatgcatca ggattgaaca
      901 actcttatct tggcgaaaac cgaccagaag gcactggaga aaacgacgaa aatcaaatgt
      961 ctgctcaaga tgtggccatc gttgcccgcc atttgatctt ggatttccca gaaatactgg
     1021 atgtatcaag tactacgacc caaatgtttg gcgaaaatac gcaatcacca gtggaaatgg
     1081 tcaactggaa ctggatgctc ccaggattcg ttaattacaa ggaaggcgtg gacggattaa
     1141 aaactggtac gactgagctt gcaggtgctt gtttcgttgg tacgatcact aaaaacgatc
     1201 aacggatcat cactgtcgta ttgaatgcgg acggccatgc ggaaaacccg agtgcccgct
     1261 tcaatgaaac agaccgtttg atggactact gctatgacaa ttggtcagaa aaagagctcg
     1321 gaaaagctaa cgcaagtatt cctagtctaa aaaccattga tgtaaaagac ggaaaagaat
     1381 caacagttcc tgttgtgctt aaaagccctg taaaagtatg ggtacgaaac gatatggata
     1441 ctggcaaatt aacgatcacg ccaacacttg acaagaagca agtcactgac aatgagatcc
     1501 aagcacctat ggataaaggt acaaaaatcg gtaccgctac gatcaccttg gcagacgata
     1561 aattaggcta tttagaagac aacaatgcac catcaacaga gatcattacg gataaaacag
     1621 tggaaaaagc gaatatattc gtgattggct ggcgacgcgt cgcagaattt ttcggcaact
     1681 tgttttaatt gaacaataag aaaaacatct ttccatcgga aagatgtttt tttgcttttt
     1741 ctagtttaaa taacagcttt tttcatctat tttcgttata ctgtataaga aaaaggagtt
     1801 gaatttatga atctaaccag agatctgatc tttttagttc tctatcttat tggggtattc
     1861 tctatcgttt attggcgaaa acagaagaag gatccttcct tgttctttta cggcagtacg
     1921 tggacattta cggtaatctt aacttatttt gtcgtgttgg aaatttttta ttcgaccttt
     1981 tattttttga ttccattgtt gttctttagt atctttgctt tttcttattt cacagaaaaa
     2041 cgaaggctac tgaatggtct tttattcaat gttttactca tcagtttcgg catctacttg
     2101 tttgtcttgc tgtatgaaac gcaaaatatt tttcttggcg gactgatcgc cttgatcacg
     2161 attccattgc tacttgtcct tttatttggt atttatggat tgattgtttt tcttttttgg
     2221 aacggggtca cagttttacg tagagaatcg cattcattag ctaatctttt aacgttgata
     2281 ctagccattt ttcttacact gtttcttgtt tttgactttt tcttgttaaa atatttgccg
     2341 caatggataa atgctttgtt tttctgcgtt cccctgatct tgatctatct atttatcgta
     2401 ttctacaact ttttgactgt ctcattcttg tatcagttca accggcctcg atacaatcaa
     2461 gacttcatcg ttgtgttagg cgctggattg atcaatggag aaacagtttc tcctcttcta
     2521 gccaagcgaa tcaataaggc aattgctttt tatcgggctc aaagccgtgc tacattaaat
     2581 ccgcccatct tattgatgtc tggcgggcaa ggagcagacg aaaaagtacc agaggccatt
     2641 gcgatgaaac aatatgccat ggaacaagga atccctgaaa gagacattct tgtcgaaacc
     2701 aattcgacaa ccactttgga aaacatgctc tattcaaagg aaatcatgga tcagcaaatg
     2761 aaagggccat accgcgcgat ctttagttcc aacaattacc acatttttag agccggtctt
     2821 tatgcgcggc aagccaaatt gaaagcaaat ggtattggtg caaaaacagc tttctactac
     2881 ttgcctaatg cgtttttaag agaatacctc gccatcttga tgatgaacaa aaaaagacat
     2941 atgatcattt gcggattgct tttcgcaggc tccgcattct tatcgctgat tactttactt
     3001 ttctaaaaaa acaaggtgtg acaaaacata tgtcacacct tgtttttttc tttcattctc
     3061 ttaactattg gcaaaaagag tatccctttt ttgaccgcta cttgctgagc agaataaata
     3121 ccgctgttgc ctgaacacat aaagctgatc aagcagatca taaagaagta tactgccgct
     3181 tgtgaaccga acaattcgat ccccatcaca aagcaggcaa tcggtgtatt tgtcgcacca
     3241 gaaaatactc cgataaagcc caatcctgca agaaaaggaa cagaaacatg gagcaaaggc
     3301 gctagtgctg caccaagcgt tgcaccaatt tcaaacaatg gtgtcacttc tcccccttga
     3361 tatcccgcgc ctaaagacaa aactgtgaag aataacttcc caataaaatc atagacatgt
     3421 gcctgtccat taaatgattc ttgaagtaac ggaagactca atccgagata acgttgtgtt
     3481 tgcaaaatca atgctgctgc cacaactaca gctccaccta tcgtatttcg acaaacagga
     3541 tgaggaatcc attgtgcata tcgcgctttt aaaaagacga tcgaacggct gaatacccaa
     3601 ccaatcaaac caaaagcaat cccagcaaca aaaactttga agaacagcaa catactccat
     3661 tcaggaatca cacccatttg gtaatgtaga tgagaaacac catagctttc tgtcatgaaa
     3721 tttgcaaaca gtgcagcaaa gaagctaggg aagatcgctt ctgttcggac tttcccgatt
     3781 gccaacactt ctaatccaaa taatgttcca gctaaaggtg tgccaaatac agaactaaaa
     3841 cccgcactga ttccactgat aatcaagatt tcccgttctg atttatccaa tcggaacaaa
     3901 cgtgccgtat tatctgctaa cgctcccccc atttggactg ctgttccttc acggccaaca
     3961 gatcccccaa ataaatgggt agttatggtt ccaaacagtg taagtggaat aagccgcaac
     4021 gggatttttt cttctccacc atttccttga tcgatcacta agttgtttcc acgactagaa
     4081 agtccgccgt gatacgcata gagataagca aaaagcgcac cactaactgg tagtagacaa
     4141 aggagccaag gatgtgatag tcggatatct gtcacaactg tcaggctatt taagaaaaaa
     4201 gcagacaagc tccccatgaa tataccgaga atcgctgtga tcaccagcca cttcagcata
     4261 tacactaaaa tgcgtactgg actcggtaat tgtttttcca taaaaaattc ctcctcaata
     4321 aaaaaacgcc tact
//
LOCUS       DACDPS010000133         4331 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.133, whole genome shotgun sequence.
ACCESSION   DACDPS010000133 DACDPS010000000
VERSION     DACDPS010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.133"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            641..1375
                     /locus_tag="IWJ07_002607"
     CDS             641..1375
                     /locus_tag="IWJ07_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9334128.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1391..2560
                     /locus_tag="IWJ07_002608"
     CDS             1391..2560
                     /locus_tag="IWJ07_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9334129.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2576..3571
                     /gene="lacD"
                     /locus_tag="IWJ07_002609"
     CDS             2576..3571
                     /gene="lacD"
                     /locus_tag="IWJ07_002609"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP9334130.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3650..>4331
                     /locus_tag="IWJ07_002610"
     CDS             3650..>4331
                     /locus_tag="IWJ07_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAP9334131.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDF"
BASE COUNT         1468 a          717 c          933 g         1213 t
ORIGIN      
        1 gtttgaaaaa tgctatttcg aggtagaagg atattatata agcacggttg actaaacgaa
       61 gtgataataa aaaaagttat tcaagaataa gaaaaattat gatacgaatt cagataaact
      121 ggagaataca gagaccttta aaagggataa gaaacaacat aaattcctct taagagagaa
      181 cgaaaggaca aagccagcaa cttgatacac gagctagtat tagaggaatt tattctcata
      241 gactcccttt tgaatacgtt tcaactttaa ttttcatgtt cataaatgat ttgaaaccaa
      301 agcaaatgcg ttgcactttt cctccgtctt ccgaaaatcg ttcaattcac tggtaagtag
      361 tcatttgata gtttgaggca aattgttgat tgatgaaggg taggagctaa aaaaagatac
      421 cagttgaaga gagaaaaaga aaaaaataga gggtgttctt gaatgaaaaa agttggaaaa
      481 ttatttttca tttcctttta ttcctattaa agtcagtgat gtcaatcata ttaacaagac
      541 taaaaatgaa aataaaaaaa agattgttct tagtattgac tcataaaaaa taccactact
      601 atacttacat tgtggttata gccagttagg agagacatcg atgcaaaata cctttcaatc
      661 aaaatcactt tattatcagc tggtagaaat attgaaacaa cgaatcgaaa acagtatggt
      721 cgctcatgac aagctgccat cagaaagaga gttgaccagt ttttatggcg ttagtcgcac
      781 gacggtgcga ctagcattgc aagaactgga aaaaatgggc tacatttatc gacaacatgg
      841 aaaaggaacc tttgtttcgg aattaaggac aaatgcgact gatctagctg gcgcatatag
      901 ttttaccgaa caaatgcggg caatgggcaa acatcctgaa accaagatat tggccttcaa
      961 gaagattgaa gcaactaaat atttagcgag tcatctgaat gttcaactag gtgagccttt
     1021 atttcaatta gaccgattgc gcttagctga tgatttgcca atgatgatcg agaaatccta
     1081 tttgccagtg aagctattta tgtccctttc tcaagaaatt ctatttctga aacctttata
     1141 taatttgttt tcagagaatt tcaatcaaat cgtacggttg gctgacgagg aattttatgc
     1201 cagcattgtc cagaaagaag atagtgagtt gctaaatatt ccagaaggat caccagtgct
     1261 ccatttagtt cgctcaacct ataatctcga gaacgaagtg attgaattta cattaagtgt
     1321 agcgcgggct gataaatttc attataagat caggcatctg cgttccacta actgaattgg
     1381 aggaaagaaa atgtttatgt tctcaaaaga agaactacga aagctaggag cagaaaatac
     1441 tacccgagaa attcgacagc aaccggaact gtggcatgag acgtttgacc gttatcaaga
     1501 aacagttgca gatttagatc gtttgtttac ggatgcgaaa caacatgctg gagagcgacc
     1561 aatccgagtc atttttacag gggccggaac ctcgcaatat gtgggagata cactaattcc
     1621 gtttcttact agtcaaggaa atacgaacca gtttattttt caaagtattg gttcgaccga
     1681 tatcgttgct acgccaaaag aatacctgat tcctgatgag acaacgatct tggtctcatt
     1741 tgcccgtagt ggaaatagtc cagaaagtct agccgcagtt aagatggcca atcagctggt
     1801 caacaagatc tatcatatcg tgatcacctg tgcaccagac ggattattgg ctacacaaat
     1861 gcctacaaaa aaagaaaatc tagttttact aatgccaaac aaagccaatg acgctggttt
     1921 tgctatgaca agcagtttca gctgtatgac gctgactgcg ctacttattt ttgatcaagc
     1981 aagcaatcag caaaaagaaa gatatgttca tcaattaagc cgactaggag aagaagtcat
     2041 ccaacgagaa tcagagatcc aagggtatct ggcatcagac tttcatcgca tcatttactt
     2101 aggttcggga agcttaggcg gattgactag agaagcgcaa ttaaaagttc tcgagttaac
     2161 tgctggaaaa atcgcaaccg tatttgatac atctttagga ttcagacatg gtcccaaatc
     2221 ttttgtcaat gagcaaacat tggttttcct gtttgtcagc aatgactcat atacgcagaa
     2281 atacgatgta gacatgctgg aagaaatcaa aaatgacggt attgcccaac gcgttgttgg
     2341 aattggtcaa aagggttcat tgtttagtgg tgattccttt gaattagttg cagattcgtt
     2401 actgccggat ggctatcttg ctttagcaga tatcatggta gcccaaacga tcgcattact
     2461 ttgttcggtg aaggttggta atacaccaga tacaccatcg ccaagtggaa cagtcaatcg
     2521 tgtggtcaaa ggcgtaacga tttatccata tgaaaaatag gaaagagtgt gaagcatgtt
     2581 agaatggtcg gaaaataaaa gacaagcaat ggaacgtcta tcgacgccag aaggcattat
     2641 taccgctttg gcgatcgatc agcgaggcgc gctgaagaaa atgatgtcgg ctttagggat
     2701 ggaagcgaca aaggaaaaaa ttgagcagtt taaaacacta atttcccaag aattgactcc
     2761 ttatgcctca gcaattttat tggatcctga atacggactg cctgctgcac agaagcgtgc
     2821 agcagcgagc ggtctgctgt tggcatatga aaaaactggt tatgatcaaa ccgtacccgg
     2881 ccgcttgccg gatctgctgt cggactgttc agttctgcga ttgaaagagt taggtgcgga
     2941 tgcggtgaaa tttttactgt attacgatta tgaagaagca gaggaaatca atcggcaaaa
     3001 gcaagttttt gttgaacgaa ttggaagcga atgcatggct gaaggactgc cattttattt
     3061 agagttattg tcatatgatg ctaaaaatgt agatacgaaa tcgctttcct atgccaaagc
     3121 aaaaccgcgc aaagtgatcg aaatgatgaa agaattctcg aaaacccagt ataaagtaga
     3181 tgtcttaaag gttgaagtac cagtaaacat gtcatttgtt gaaggaaatg caacagggga
     3241 gatcgcctat acacgtcaag aagctgccgc ttattttaaa gaacaaagcg atgcaacaga
     3301 tttgccgtat atcttcttaa gtgctggggt atcagcggca ttattcatgg agacattgcg
     3361 ttttgccaaa catgctgggg cacgattcaa cggtgtactc tgtggcagag caacttggaa
     3421 agatgcagtc gctcctttcg ttgaaaaagg agaagcggca actttagaat ggctgacaga
     3481 acaagggaca caaaacattc gtcaattgaa tgaagtgata agagaaactg ccatcccatg
     3541 gtatgagaaa gtaagtgaaa gcacttgtta atgtttctaa tatattagta gtgataagag
     3601 atatgtgtga ttataattta tcattattga aactgggagg atattttata tgaataaaag
     3661 attgttagat tattcagctt cagatttttt gaaagctaag ccgatggaat taaagcaggc
     3721 aattttggct tctgaaggta gaacaattat ggcagaaaat gtgccaagta gctgtcctta
     3781 catgggaaaa tcagtgacaa atgcagaatt ggagagagct gctggagctg atttaatttt
     3841 gtttaatgca ctagatcttt ttaatcctca aatttcaggt gtacctttaa ctctaaaaga
     3901 gaatccaata atctggatta aaaaagcttt aggaagagct attggtgtaa atttagaacc
     3961 tgtagacaaa gaagttttga tgagtgaaaa tcgaactgaa atttcaagtg gtagaatcgt
     4021 ttctcctgag actttaaaaa aggcaaatga attaggcttt aattttattt gtttaactgg
     4081 aaatccagga acaggagtat ctaatagaac tattgagaaa gcaataacat tatccaaatc
     4141 aatttttaat ggattaatta tcgctggaaa aatgcatggt gctggtgttg atgaaccaat
     4201 tatgaactta gatatagcaa aaagatttat taaaacaggt gtagatattt tattagttcc
     4261 tgctccatac actgttccgt atttcatgga aagtgatttg cgaggaatcg ttgattatat
     4321 aaaagatttt a
//
LOCUS       DACDPS010000134         4245 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.134, whole genome shotgun sequence.
ACCESSION   DACDPS010000134 DACDPS010000000
VERSION     DACDPS010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.134"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(90..446)
                     /locus_tag="IWJ07_002611"
     CDS             complement(90..446)
                     /locus_tag="IWJ07_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334132.1"
                     /translation="MFLYLFAMAEEKSGLGLKKSFLVWIRYFRKSSSFRKMFLLVFFT
                     NMILFRTLMDRYVWLNPLSNVFGSWWIYSVNETTGEIRLTTECFENVILFIPFIILVM
                     WYKASEKDFGKIYQRL"
     gene            complement(1767..2483)
                     /locus_tag="IWJ07_002612"
     CDS             complement(1767..2483)
                     /locus_tag="IWJ07_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334133.1"
                     /translation="MTVIITMAGLGSRFRKVGYTVPKFMIEAKGKTLFDWSLDSLLDY
                     NSHVEKYVFVVRKEDDATVFIKEHCSLYGIENVEVVEIDYMTDGQATTCMLAIPYCAP
                     DNAIMVYNIDTYVEPGEMKYVDISGEGHIPCFHADGDHWSFAKLNEYGDVVEVREKVR
                     ISNNCTLGAYYFSSAKLYASLYEEYYADERHLEKNEKYIAPLYNYMIEKGMKVTISIV
                     DKSKVHVLGTPEELDVFLNE"
     gene            complement(2508..2906)
                     /locus_tag="IWJ07_002613"
     CDS             complement(2508..2906)
                     /locus_tag="IWJ07_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HAP9334134.1"
                     /translation="MFKDYAFVFDIDGTLCPIKKKDEKYEDLVPYANMVEKLRYYKEN
                     GAKITLFTSRNMNSYHGNLGVINANTAKILLAWLDKWEIPYDEILYGKPWPGHKGFYV
                     DDRTVRPDEFLSCTLDQLDEICKKSKLKSE"
     gene            complement(2994..>4245)
                     /locus_tag="IWJ07_002614"
     CDS             complement(2994..>4245)
                     /locus_tag="IWJ07_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HAP9334135.1"
                     /translation="TFFEEKDGDIISVLDKNELKEDDGKKHKLFSGVFQIMDAQYFRE
                     CLRKALMSNVVNVNSFYQALQEYSKRYEFLSIKTNNWFDIGHADKYYNSKLEVKAREF
                     NHISIDKDRSILRKISEDVEKFIGEIKWYLKLPAQVEYVRPRIFEYSTSYINPYVSME
                     YYSYHTVHELFLYSDLTKKQWIDIFNRIRFVCSDFKRYSVSGDNIQKSLKDMYLDKTF
                     QRFNKLRKDPRFTEFFSSDIQINGVRYKSLDQIEALLSVSVPRELFDITQFNIIHGDL
                     CFANIMVDNTFSFIKVIDPRGKFGDFDIYGDYRYELAKLFHSVDGKYDFIIKDLFTIK
                     YDPKKAIIDYIVQDRKRDYDLYEVFYSVFKDEIGSDLKKIELIEALLFLSMIPLHGES
                     LNHQMAMLATGLEILGRVVPDIYC"
BASE COUNT         1516 a          840 c          534 g         1355 t
ORIGIN      
        1 attataaaat aaatctgaca attggaatgt ctctatgtgt aaaaataact gcaaaaactc
       61 tataagacac gaacataaaa aaacaaattt tataatctct gataaatttt tccaaaatct
      121 ttttctgaag ccttatacca cattactaga attataaatg gaataaacag aataacattt
      181 tcaaaacatt ctgtagttaa tctaatctcc cctgtcgttt cattcacaga gtaaatccac
      241 caactaccaa acacattaga caaaggattt agccaaacat atctatccat cagtgttcta
      301 aaaagaatca tatttgtaaa aaacacaagc aaaaacattt ttctaaatga gcttgatttt
      361 ctgaaatacc gaatccagac aagaaaagat tttttcaaac ctaatcctga tttctcctca
      421 gccatcgcaa acaagtataa aaacatagcc aaaactgcaa ttaaaatcga aacccaaata
      481 tttgatacaa tgcagataac acattaacca aaatagaaga tatgattttc ttcataaact
      541 ctcctttcat catgcgctaa atgagtacca tcaagaaggt ttcgtaaaag gaggtaccca
      601 ctctaacatg aataacctcg gccagcgaaa ataaaactca acagaattcc tatctctatg
      661 ctgctataat ttcaacaaat ttacatgtac cagttactaa tctaatataa ttttttcagc
      721 aaggtaaatt tcacactaca cgcatacaca aaaactgtag aaattaaaat tattatcatt
      781 ccacaagtca tttctattaa tgaatttgta atccccaatt ttgggaaaat aaaatttaaa
      841 gcagctatta tatacggatg taaacaaaat aataacgtac taattcttcg cagatgtact
      901 gttattttta taaatgtact atctttaggc tcagtgatac tgatacaact accagtgcta
      961 atggaatgga aaatattaac aaattattga cttctcgcaa agctatttga tttatgatga
     1021 aactttcacc aactaataag catcctatag ctaaaacagc acataagata aaattacggt
     1081 atttaaagac cttattaagt atagtgtttt tgtggaaggc taagtgttgc ccaataatga
     1141 cgcatggtac acctataaat aatccattac gagttgttac aaacacgctt ttataacaat
     1201 atctaatata ctcgatggaa tcattcccag ataatataaa ccaataagat tcaaatgcta
     1261 aacctactac gcatagcaac aaaccactga gaaactttat tttagaaacc tggcttttgc
     1321 gtactaacca tagagcaaaa gccagtgcag gcaagaacca caggtgatag taactaccaa
     1381 taaacagaaa atctctgata tacaataata catctgtttt ccagctaaaa tctgactgta
     1441 cccaattctt taatactaat ggaatatata atatagacca aataatgtaa attttaagta
     1501 ctctgtagat ttgtttagat aatttagcgg atgtaacttc accactacac aacaaaaagg
     1561 aactgatgat gaaaaaagta ggaacggcaa ccgtactcag tcctgcaaca ataccaatac
     1621 taaatgctta atctggagct aaatagtttt gacctaaatg acgaacaaca actaaaatag
     1681 ccattagtat ttttagaata tctatcgaat agtactcccc cacacacgca gctttataat
     1741 taacattttc tatgattata ttttccctat tcatttaaga acacatcgag ctcttctgga
     1801 gtaccgagta cgtgaacttt tgatttatct actatactaa tggttacctt catgcccttc
     1861 tctatcatat aattatatag cggcgcaata tatttctcat tcttttccag atgcctttca
     1921 tctgcataat attcttcgta gagtgatgca tataattttg cagaagaaaa ataatatgca
     1981 cctaaagtac aattgttaga aattctaact ttttctctta cttctactac atcaccatat
     2041 tcgttcaact tggcaaaact ccaatgatca ccgtccgcat ggaaacacgg aatatgtcct
     2101 tctccagaaa tatcaacgta tttcatctca cctggctcaa catatgtatc tatgttataa
     2161 accataattg cattatcagg tgcgcaataa ggaattgcaa gcatacatgt agtggcttgt
     2221 ccatcagtca tatagtcaat ttcaactacc tcgacatttt caattccata cagactgcaa
     2281 tgttctttga taaaaacagt agcatcatct tccttacgca caacaaaaac atacttttct
     2341 acatgtgaat tataatcaag caagctgtct aaggaccaat caaataaggt tttacctttt
     2401 gcttcaatca taaatttggg cacagtgtag ccaactttac gaaagcgact gccaagacca
     2461 gccattgtaa taataaccgt catcttattc actttataaa gtctccttta ctcagatttc
     2521 aatttggatt ttttgcaaat ctcatcgagc tgatccaggg tacacgataa aaattcatcc
     2581 ggacgaactg tgcgatcatc aacataaaac cctttatggc ctggccaagg ctttccataa
     2641 agaatttcat catacggaat ctcccactta tctagccaag ccaacaagat ttttgcagta
     2701 tttgcattga tcacacctaa attaccatga taagaattca tattgcgaga tgtgaacagt
     2761 gtaatctttg ctccgttctc cttatagtat cgcaacttct ccaccatatt agcatacgga
     2821 actaaatctt catatttctc atctttcttt ttgatggggc acaaagttcc atcaatatca
     2881 aaaacaaatg cgtaatcttt aaacatataa ctaagacatc tcctttattt tagccaggca
     2941 atttatatgc ccggctttac actgatcaaa tagaaaaaca atagctcaaa gcattaacaa
     3001 taaatatccg ggacaactcg tcctaaaatt tccaaaccag ttgctaacat agccatctga
     3061 tggtttaagc tttctccatg caaaggaatc atacttaaaa acagcaaagc ctcaataagt
     3121 tcaatttttt tcaaatcaga acctatttca tctttgaaga cagagtaaaa tacttcatat
     3181 aaatcataat ctcgtttacg atcttgaaca atatagtcta tgattgcctt ctttggatca
     3241 tattttattg taaacaagtc cttaattata aagtcatatt ttccatcaac actatgaaac
     3301 aattttgcca gttcatatct ataatcacca taaatatcaa aatctccaaa cttacctcgc
     3361 ggatctatga ctttaatgaa cgaaaatgtg ttatctacca taatattggc aaagcataag
     3421 tcaccatgaa taatattaaa ttgcgtaatg tcaaacaatt ctcttggaac acttacgctc
     3481 aataaagctt ctatctggtc caaagattta tatctgactc catttatttg aatatcagaa
     3541 gaaaagaatt ctgtgaaccg ggggtccttt ctgagcttat tgaatctctg aaatgtttta
     3601 tcgagataca tatctttcaa tgacttttga atattatccc ctgaaacaga gtatctctta
     3661 aagtctgagc atacaaatcg tattctatta aagatatcta tccattgttt ttttgttaag
     3721 tcactataga gaaataactc gtgaacagta tgatacgaat agtattccat acttacatat
     3781 ggattaatat acgaagtaga atattcgaat attctaggtc tcacatattc tacctgtgct
     3841 ggcaacttaa gataccactt aatttctccg ataaattttt caacatcttc actaattttt
     3901 ctcaggatgc tacgatcttt atcaattgaa atgtggttaa attctcttgc cttaacttcc
     3961 aatttggaat tataatattt atctgcatgt ccaatgtcaa accaattatt tgttttaata
     4021 gagagaaatt catatcgttt gctatactct tgtaaagctt gataaaaact attcacattt
     4081 actacatttg acatcaaagc tttacgcaaa cactctctaa aatactgggc atccataatt
     4141 tgaaatacac cagaaaacag tttgtgcttc tttccatcat cttcttttaa ctcgttctta
     4201 tctaaaacag aaataatatc tccgtctttt tcttcaaaga atgtc
//
LOCUS       DACDPS010000135         4207 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.135, whole genome shotgun sequence.
ACCESSION   DACDPS010000135 DACDPS010000000
VERSION     DACDPS010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.135"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            142..1602
                     /locus_tag="IWJ07_002615"
     CDS             142..1602
                     /locus_tag="IWJ07_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334136.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIREIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            1823..3586
                     /locus_tag="IWJ07_002616"
     CDS             1823..3586
                     /locus_tag="IWJ07_002616"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAP9334137.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            3670..4128
                     /locus_tag="IWJ07_002617"
     CDS             3670..4128
                     /locus_tag="IWJ07_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAP9334138.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
BASE COUNT         1483 a          632 c          848 g         1244 t
ORIGIN      
        1 tgtgagcttg tttgactttt gtcccagttt ctttttttat tggtttttga aagggctttt
       61 ctaaataaaa aggaaacaaa aatacgattt acagatatga ttaaataaaa tcataagaaa
      121 aactggaggc gtgtatcagg aatgctggaa aattacatcg agcgaaatat atttagaaaa
      181 gtgtatctgt gtgaacaatt atttgaattc caagaaatag acatcgaaca aacagctatt
      241 tctctaaggg tgactacacc aacgatcctt cacgatctag aaagtctagc cgagtgtcta
      301 gaatattgta taaaagaaca agtaagagag aagcataagt ataaactcgt attcaaacat
      361 ggtattgctc tttctgagct cacacagttt ctgtacggtc aatcttattt tttaaagttt
      421 ttaagttatt attttaatgg tatattcacg agtacagaac tggctgatct tgaatttatt
      481 tctttgagta aagtatatac aatcaaaaaa atcgtattgg atttttttaa agaaaatgat
      541 tatttgaaaa ataaagaaat cattatccca gaatttgact cgcggaacat cttgctagct
      601 ttagtacgtt atgtcaattg ggaaggctat gaaaataaaa atccgtatgt agaagaagct
      661 tgtaacgagt tgattgatta tgtagagact cattttttta aaagaagata ttcggaagat
      721 gaaaagtcct ttatcatacg agaaatagag atcgcaatcg gaagaaaggc atttcccctt
      781 ggactgaaag aagaagataa gaaaagcgca gaaacgaagc cgttatttca attagtctgt
      841 caaggattgg aagagcaaaa aggaattcta gacttacaag aagaagatgt ctattatatt
      901 ttttctttgt tcaacagtag aaattatacc aatgagaaca tggagctgtt aagaaaagat
      961 ttcgaagttg tttataaaaa ttttgttctc aatgacctat cactgaatga attgatttta
     1021 cagattatta gtatcgctga aatcgacgat gcagaaaatt ttattttcaa gcaatccttt
     1081 ttacaatttg tccgtacgtt gtgggcagat ggacaagtat ttttaccgga aaggatctat
     1141 cttctatcag aaaaagaaca acttctgtac ggaaaaattg tagaaatatt ggaagagtgg
     1201 aaaaagaaaa atcaaatcca actaagatgg aatgaaaatt tcattcgaaa attcgtcaaa
     1261 agtctttctt tgatcaacag tgaacaagcg gggagacagg aaatagaaat ttttgttgtt
     1321 tcagaaagtt cagtcaaaca atttttttat cgaacccaat ttcatttgta tgcggaagaa
     1381 ggcatcgctg aaataaatcc tattttattt cgttcattag aagaattgcc tgatgaatgt
     1441 ctatgtgcga aaaaaagagt ggttttgtgc gatgctgcta gctatcaaaa tggattagaa
     1501 actgaaaaga caaagatcta tccaatctca ttaaaaggtg caaacatcca tttgtatcat
     1561 attgctcagg aacttcacct attaaaaaaa catatttctt gatgagacct taattaggag
     1621 accttctaca aagcttttta attgtctgta gaaggttttt atttgagtcc tgattcgtgc
     1681 gaagatctac ttatataaca agctttctct gaaatttttt taaaatgcta ttgacttaga
     1741 gtcaactcta agtagtagaa tagataaagt gtaacaaatc tattttcatt aaaagaagat
     1801 agtacggagg agagaatgta ttatgagtac agtattacat gtattggatg caatcaatag
     1861 ccccgcagat gtcaaaaaat tatctgttga ggaaatgaaa cagttggcag aagagatgcg
     1921 ccaaaaaatc ttgcaaaagg acagcgatgt aggtgggcac gttggtccga atctaggtgt
     1981 agtagaatta acgattgcgt ttcattatgt atttgattca ccaaaggata aaatcgtttg
     2041 ggatgtttcc catcagtctt atggtcataa gcttttaact ggtcgaaaaa atggttttga
     2101 aaaaggacat taccgtgaag tgagcggata ttccaaccaa catgaaagta aacatgacta
     2161 ttttacagtg gggcacacat ctacatctat cagcttagca acaggtttag ctaaagcgag
     2221 agatctgaag aaagaatcag gaaatgtggt agcatttatc ggtgacggct ccctaagtgg
     2281 tggcttagct tttgaaggat tgaacaatgg tggtgtttta caatccaatc taatcgtatt
     2341 agtaaacgat aatgagatgt ctattgatga gaatcatggc agtttatata aaaacttagc
     2401 tgagcttcgt caaaccaacg gtaaaagcag caataaccta tttaaagcaa tgggatttga
     2461 ttatcgttac gtggataatg gaaacgatct ggaaacggtg atccgtttat ttgaagaagt
     2521 aaaagatatt gatcatccga ttgtacttca cgtccatacg gaaaaaggtc gcggttatca
     2581 gccggcaatc gataataaaa tgaaatatca ttggcatgta ccatttgatc tgtccaccgg
     2641 ggaggcaaag caagtaactt ctggagaaac atttaatatg atcatcatgg attatttgga
     2701 taagaaaatg caaacaagta cagctcctat tgccgcagtc aatgcagcga taccaggcgc
     2761 ctttggactg aaaacattcc aagaaaaaca tccagatcat tattttgacg tggggatcgc
     2821 tgaacagcac tctatcactt tcgcttctgc tttggcttct cagggagtac gtccagtcgt
     2881 cttccaatca agtactttct tgcaacgggc atatgatcaa ttatcacatg atttagcgat
     2941 caacgagaat ccagtgatta ttttagtcgg tggcggaacg atctcagcta gtgaccgaac
     3001 acaccaagga acatttgaca ttccttactt atcaagtatc ccgaacatca aatatcttgc
     3061 tccagcaaca aaagaggata tgatctctat gctagattgg gcgttcgaac aaacagcgca
     3121 gcctgtagca atccgaattc ctacacatgg tgtagaacat ggtacagcta tcgcaaatga
     3181 ttatagtatt ccagcctatc aaacagtaaa taatggagga aaagtagcaa ttttagcttt
     3241 aggcggtttt cttgaattag gaaaagaaac agtaaaagct tttagaaaaa aaggaatcga
     3301 agcaacgttg atcaatccag gaacgatcac agaaaatgac gtagaaacat tagataaatt
     3361 aaaagaaaca cacgaattag tcatcacatt agaagatggt agtttgtcag gaggatttgg
     3421 cgaaaatatc agtcgttatt atggtgattc ttcaatgaac gtattaaatt ttggtgcaga
     3481 gaaagaattc acggatagcg ttcctgtagg gcagctttat gaacgttatc atttaacacc
     3541 aaaacaaatc gttgaagata gtctgcgtgt cttgaatatt caataattca ttatgctcac
     3601 catccagata attgtttata ctagtatctg gatggttttt tcattataaa aagcaaaggg
     3661 gtggatgtta tgtatcagca agtagaagaa tatgtcaaaa agttccaaga aaaatatcct
     3721 agttatcaat cagaaaaatt aggtttcttt tcatttgggg atacagaaaa aatggcagac
     3781 gagttgagcg aactagttgt aaaaaagata aagactggta catcaagtgg ttacgatctg
     3841 tataaacaag agaatgagcc tttaccaaaa gttggtcaat tagacgtggt cctgaatgga
     3901 aaaaatgaac cagtttgtat catcaaaaat ataaatgtga ccatcttgcc ttttagtgaa
     3961 gtcacgaaag agcatgcttg gaaagaagga gaaggggatc ggacattagc ttattggcgg
     4021 aaagtccatg ctgagttttt taaacatgct tataaagaag cgttgaacat agaatttaca
     4081 gaagagaagc tggtcgtata cgaagaattt gaagtaattt tcttttagta agaaaggtct
     4141 gctgaaagaa atagctcatt attgtctcct gaatagaaaa gggttacatt tttatatgaa
     4201 gctgtat
//
LOCUS       DACDPS010000136         4047 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.137, whole genome shotgun sequence.
ACCESSION   DACDPS010000136 DACDPS010000000
VERSION     DACDPS010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4047
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.137"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..351)
                     /locus_tag="IWJ07_002618"
     CDS             complement(<1..351)
                     /locus_tag="IWJ07_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334139.1"
                     /translation="MKLRANIIEKRGTLQVVVSYKDSNGKIRQKWKDTKLKKKGNKKR
                     AEKIRDEFLLELESQLAMECECANPNLLFYDYLLNYLEVSKKQVAFNTYVGYKHYVHN
                     RIYKFFYPKKIKLNR"
     gene            complement(395..625)
                     /locus_tag="IWJ07_002619"
     CDS             complement(395..625)
                     /locus_tag="IWJ07_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9334140.1"
                     /translation="MDFQDYEKIFESYPSILKITDLTKMLNIGKNKAYNLLKDRSIEN
                     IKIGARYKIPKKAVIEYILKIQDEGKNHAIIK"
     gene            701..773
                     /locus_tag="IWJ07_002620"
     tRNA            701..773
                     /locus_tag="IWJ07_002620"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:734..736,aa:Lys,seq:ctt)
     gene            935..1156
                     /locus_tag="IWJ07_002621"
     CDS             935..1156
                     /locus_tag="IWJ07_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334141.1"
                     /translation="MRNEIANITIIRETLQNHTANEISKHTGLNLSTIKKLKSGERSI
                     EKLNLHDAICLTEFGLKNNRKNVEINIWK"
     gene            complement(1480..1725)
                     /locus_tag="IWJ07_002622"
     CDS             complement(1480..1725)
                     /locus_tag="IWJ07_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9334142.1"
                     /translation="MVDNPKKRQYPLVPLSIIQSALEEDPISIKMILKHYHSYIAKLC
                     LTNGFNEAGQFITYVDEYMLRQLEIKLIEAILKFKIN"
     gene            complement(1719..2120)
                     /locus_tag="IWJ07_002623"
     CDS             complement(1719..2120)
                     /locus_tag="IWJ07_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9334143.1"
                     /translation="MQQNYNEKSIQHQFDAYCKKVLRNETKSIRKRNINITDNEEPLT
                     NSNEGKYSQTHFDEQDVYFLFGMEILISDQKLSAAIDHLSEIRRKVILLYYFAGFNDT
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(2292..>4047)
                     /gene="uidA"
                     /locus_tag="IWJ07_002624"
     CDS             complement(2292..>4047)
                     /gene="uidA"
                     /locus_tag="IWJ07_002624"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAP9334144.1"
                     /translation="WRFKLDTNDDLQEELALEPMVEKESYPISVPSSYNDLFETENIR
                     NHVGWVWYEKEVILPKKLLEERLVLRFGSATHEAKVFLNGKLINQHKGGFTPFESEIN
                     QFIKDGSNRITVAVNNVIDDSTLPVGILKETIQEDGDIKKENLVNFDFFNYAGINRPV
                     KLYTTPKDYIKDVTINTKIIDSDANVEFNIDTEWTGEILIAIYDEEAQLIAEAKGKEG
                     VIQLKNPTLWEPLNAYLYSMKVSLINENKVIDTYTEEFGIRSIQVSNGKFLINNKPFY
                     FKGFGKHEDTYINGRGLNEAVNIKDFNLMKWIGANSFRTSHYPYSEEIMRLADREGIV
                     VIDEVPAVGLHLNFMATLLDDNVEKHDTWKEIRTENAHKMAIKELIERDKNHPSVVMW
                     SIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYLKSTPDICKVGDIVDVLCL
                     NRYYGWYVSGGDLKHAKYLLKKELDGWMERCPDTPIMFTEYGADTVAGLHDTVPTMFT
                     EEYQVGYYEANHEVIDKCDNFVGEQVWNFADFETSQGILRVQGNKKGIFTRDRKPKMI
                     AHYLKKRWSSIPNFSYKK"
BASE COUNT         1253 a          733 c          525 g         1536 t
ORIGIN      
        1 cctatttagt ttaatttttt taggataaaa aaacttgtaa attcgattat ggacgtaatg
       61 cttataccca acatatgtgt taaatgctac ttgttttttt gatacttcca aataattaag
      121 caaatagtca taaaacaata aatttggatt agcacattca cattccatag ctaattgact
      181 ttccaattct agtaaaaatt catctctaat tttttctgct cgttttttat ttcctttctt
      241 ttttaatttt gtatctttcc acttttgtct aatttttccg ttagagtctt tataagatac
      301 aactacttgt aatgttcctc ttttttcaat tatgtttgct cttaatttca attcaatctc
      361 tcctcttaaa ctgaaatatc ggcaaaaatt ttgactattt tattatagca tgatttttac
      421 cttcatcttg tattttcaat atatactcaa tgacagcttt tttaggtatt ttgtaccttg
      481 ctccaatttt tatattttct attgatctat ctttcagcaa attataagct ttattttttc
      541 caatatttaa cattttagtt aaatcagtaa ttttcaatat cgatggataa ctttcaaata
      601 ttttttcata atcttgaaaa tccataaaat tcactctcaa ttctgataat aagtattgca
      661 cttttttatt ttaggtgtta gaataacaaa gtattaattt gggctgttag ctcagttggt
      721 agagcagatg actcttaatc atcgggtcgc gggttcgagc ccctcacagc ccattgggtg
      781 ccaaaccctt aaatacagtg taaacgttgg tgcttagcat ttagaaaacg tatatactgt
      841 atttttttgt tttcaaaaga gactgtagta cccctttttt tcactaaaag cccactgtag
      901 tcattgatat ttttgataag aaaggaacat aattatgcgt aatgaaattg ctaatataac
      961 aataatccga gaaactttac aaaaccatac tgcaaatgag atctctaaac atacaggatt
     1021 aaatttgagt actataaaaa aactaaaaag tggagaacgt tcaatagaaa aactaaattt
     1081 acatgatgct atttgcctca ctgaatttgg tcttaaaaat aatagaaaaa atgttgaaat
     1141 caacatatgg aaataattct taatcagctg cttttgtcaa aagcacatag gaatttcacc
     1201 tctctccaag tttacggcga gccttacgga agtatcatta tcccaacttc aaatcatcgc
     1261 tttttattga ctataaaaat tattttagaa tgtttatcgc tccttcttta cagtaagaaa
     1321 tcatggcgca actaaaatat gctccttgaa gtgttaacgt actgatagaa tgtatttaaa
     1381 tttcaatgat caataggttt tctgtttaaa agaatctact aatcctttaa ctattagaag
     1441 attcttttaa acagaataag tggaaagggg actttttagt tagttaattt taaattttaa
     1501 gatcgcttct atcaatttaa tttctagctg tcttagcata tactcatcta cataggtaat
     1561 aaattgcccc gcctcattaa atccatttgt caaacataac ttagctatat aactatgata
     1621 atgtttgagt atcatcttga ttgaaattgg atcttcttct agagcagatt ggataatgga
     1681 tagaggaact aatggatact gccgtttttt ggggttgtct accataagtc ataccccatt
     1741 tttaattgtt caactgcctt ctttcgttga taccagatac cacttgtact catattgagg
     1801 attttaccga tttcagtatc attaaaacct gcaaaataat aaagtaggat aacctttctt
     1861 cttatttccg ataaatgatc aatggctgcc gataactttt gatctgaaat taatatttcc
     1921 attccaaata agaaatagac atcttgttca tcaaaatgag tttgggagta cttaccttca
     1981 tttgaattcg tcaatggttc ctcattatct gttatgttaa tgtttcgttt tcgaatagac
     2041 ttagtttcgt ttcgtaatac ttttttacag taggcatcaa attgatgttg tatagatttc
     2101 tcattgtaat tttgttgcat gataaatatg ctcctttttt cccctttcca ctaatacaac
     2161 aatcagaaag cgcctttttt tcgaaaaaac tagaaaaaaa tatccttctt taaacagaaa
     2221 atctttttat aaaaaaaagg agttggcaat taagcagcca ctcctttttt atattcctta
     2281 taaaaggtaa attatttttt ataactgaaa tttggtattg atgaccatcg tttctttaga
     2341 tagtgagcaa tcattttagg ctttctatct ctagtaaaga ttcctttctt atttccttgc
     2401 actcttaaaa tcccttgact tgtttcaaaa tctgcaaaat tccacacttg ttcaccaaca
     2461 aaattgtcac atttatctat tacttcgtga tttgcttcgt aataacccac ttgatactct
     2521 tctgtaaaca ttgttggtac tgtatcatga agtccagcta ctgtatctgc tccatattct
     2581 gtaaacatta ttggcgtatc gggacatctt tccatccatc catctagctc ttttttcaac
     2641 agatattttg catgttttag gtctccacct gaaacatacc aaccataata gcgatttaaa
     2701 cataaaacat cgacaatatc tcctacttta caaatatctg gtgttgactt taagtaagta
     2761 acgattgtta caggtctctt ctgagggtca agttctttag tcaattcaac taaaggttca
     2821 aaatattctt tagcgccttc agaatccgaa tctggttcat ttgcaataga ccacattacg
     2881 acactcggat ggttcttgtc gcgttctatc aattccttaa tcgccatttt gtgagcattt
     2941 tctgtccgta tttctttcca agtatcatgt ttttctacgt tgtcatctaa taaagtagcc
     3001 atgaaattca aatgtaatcc tactgcaggt acttcatcaa taactacaat accttctcga
     3061 tctgctaagc gcataatttc ttctgaataa ggataatgtg aagtcctaaa tgaattagca
     3121 ccgatccatt tcattaaatt aaaatctttg atatttactg cttcattcaa accacgacca
     3181 ttaatatacg tgtcctcatg cttcccaaat cccttaaagt aaaaaggttt attattaatc
     3241 aaaaacttcc cattcgaaac ctgaatacta cgaatgccaa attcttctgt gtacgtatca
     3301 atcactttat tttcgttaat taaactaact ttcatactat ataagtaagc atttagaggc
     3361 tcccaaagtg ttgggttctt aagttgaatc actccttctt tccccttagc ttctgctata
     3421 agttgagctt cttcgtcata gattgctatt agaatttctc cggtccattc tgtatcgata
     3481 ttaaattcta catttgcatc tgaatcaatt atttttgtat taatagtgac atcctttata
     3541 tagtctttcg gagttgtata caatttaacg ggtctattaa tgcctgcata attgaaaaag
     3601 tcaaaattaa ccaaattttc tttttttatg tctccatctt cctgtatcgt ttctttcaaa
     3661 atccctacag gtaaagtgct atcgtcaata acattgttta cagccactgt aattctgtta
     3721 cttccatctt ttataaactg attaatttct gattcaaatg gcgtaaaccc tcctttgtgt
     3781 tgattaatca attttccatt caaaaatact tttgcttcat gtgttgcaga tccaaaacga
     3841 agaactaatc tttcttctaa taatttttta ggaagaatca cttctttttc ataccacacc
     3901 catccaacat gatttctaat gttctctgtt tcaaataaat cattataaga agaaggaact
     3961 gaaattggat aactctcttt ttcaaccatt ggttctaaag caagttcttc ttgtaagtca
     4021 tcatttgtat ctaacttaaa tctccaa
//
LOCUS       DACDPS010000137         4041 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.138, whole genome shotgun sequence.
ACCESSION   DACDPS010000137 DACDPS010000000
VERSION     DACDPS010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.138"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            179..775
                     /locus_tag="IWJ07_002625"
     CDS             179..775
                     /locus_tag="IWJ07_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP9334145.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            899..2698
                     /locus_tag="IWJ07_002626"
     CDS             899..2698
                     /locus_tag="IWJ07_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP9334146.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2976..3188)
                     /locus_tag="IWJ07_002627"
     CDS             complement(2976..3188)
                     /locus_tag="IWJ07_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334147.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
BASE COUNT         1188 a          688 c          712 g         1453 t
ORIGIN      
        1 tttaaaaaaa acaagttatt aaataagtct ttagttggat gtttttttga gatcaatctt
       61 ttatacttat gatgaactta caaaattgtt tgttttctgt catatcaaaa aatggcgcag
      121 ttagttgctt ttctagtaaa gtatagttaa caaagaacct ctaaggagtt gatgaataat
      181 gaatattgct gcctctcgaa aactgataaa tttcatatcc attatcggat taggcctatc
      241 aatcgcgctg acgatttatt ttattaattt aggtgttttc aaagatttga atgcgttaag
      301 aggattagta ggtgattcca tcattttagg accgattatt tttgtcttca tccaaatcct
      361 gcaagtagtt attccaatca ttcctggagg gatcagtact gcagctggtg tcctgatatt
      421 tggcccttat gctggattta tctataacta tattggaatc tgcattggtt cgattatcat
      481 ttttctgcta gggcgtcgtt atggaaagcc attcatactt tccatgatta gcgataagac
      541 atataacaaa tatattggct ggctggataa tcaaaaccgt tttgagaagc tgtttgctct
      601 agctattttt cttccagttg ctcctgacga tgcgttatgt ttgatggctg gattgacaaa
      661 catgtcagtg aaacgatata cactaatcat tttgattgct aaaccactgt cgattttctt
      721 atatagtatg gccttgattt atggaggtca atatctaagt ggtcttcttg gataatattt
      781 attttgtaaa attagtcatt gattgacaat gttaaatctg ggaaagggtc tgcaaaacac
      841 actgcaccct ttcctttttc attagatttg cgctataatg tgaagagcag gtgataggat
      901 gaattatttg aaacgtagct taggtaatgc ttgggacttt ttaaaaggaa cacaagctta
      961 tttccgtgat gtcttgctga tgcatggctt tattttattt atttgtttac ccttattgag
     1021 cagtacgacc cgttttattt tacaccgagg ggcaattgac tatttgtctt atgacaatat
     1081 tccttcaatt ttttctcaac atcctggggt tctactctcg ttaatcgtag ttttgatgac
     1141 gattttactg ttagtttatt tcgaatttac ttttcttttg atgagtgttt tttttattaa
     1201 aaagaaagaa ccaatctctt tgaagcaact cttacacttg acgatacttc agctgaagaa
     1261 agtgagaccc attacttttt tgtttttcct tgcttatttt ttattgattc taccgatcag
     1321 cggattaagt tttaattctg atctactgtc aaaaataaaa atccccgctt tcattatgga
     1381 ttttattttt gctaatcgtt ggatcatcgt cagttccttt ttactcgttt atattttcct
     1441 tggctatatt gggattcgac tgatttttgc tttaccggaa atgattctac gggatcgtcc
     1501 ttttcgagca gccattcggg aaagctggtc attgaccaaa tcgcgtcttt tggccatcac
     1561 gggccaattt attgtgattg gcggtacgat tttactcctt tcttctttag gatatatcgt
     1621 tgttattttg gcacaaagta tggttgaaca atttttccca gactatgcat tgatcagcgc
     1681 tgtgtttgct atgacgcttt tacaagggat tttattattc aatatcgtga tgtctaccgt
     1741 agggattttt tatatcattg ttgatttcat ggatgacgaa ggcttcttgc ctgagatccc
     1801 taagtggttt atcccacaag cccccaactt gcgtttctct gcgctgaaaa acactggttt
     1861 gactcttttt gcagtttttt tcggtattgg cgtctgtctt tacaatatgg attatctgac
     1921 ttcagctgtt cagacaaaac cagtgactgt ctctcacaga ggtgtgagta cccaaaatgg
     1981 ggcgcaaaat actttagaag cacttgaaaa aacaagcaga gactatcatc ctgattatgt
     2041 agagatggat gttcaggaaa caaaagatgg tcatttcgta gtgatgcatg atgcaaatct
     2101 tcgtcactta acaggggtaa atggaacacc acaggatctt acattgaaag aattgacaaa
     2161 tcttacagtt actgaaaacg gagtgcaagc ccctgtttgt tcttttgatt cttatttgga
     2221 aaaagcacat aagctaaacc aaaaattact tattgagatc aagacctcta aaaaagattc
     2281 ggatgacctt gtcgaacgat ttgtccataa ataccgcaaa gttatcttag agaaccatca
     2341 tattttgcaa agtttgacgt accaaaccgt ttcagattta aaagaagcag aaccaaactt
     2401 ttatgtgggc tacatcttac ctttcaatat tgttgggccc cctgtgacgc ctgctgattt
     2461 tctgacaatg gaatatagta cgatcaaccg taacttcatt aattctgccc accaagacgg
     2521 aaaagaagta tatgtatgga caatcaacga ttcagatgct atgagtcgca tgatgtttta
     2581 tggtgtggat ggtatcataa ccgatgagat gggactacta aatgaaagtg tgaaacaaac
     2641 agatgaagaa atcacttatt cagataaact gctaaatttt gttttgggag tcggctaatc
     2701 aaaaatcgtt aatgaaaaaa gtctttatac aacaaaaaac gcataacctg ccaccgcagg
     2761 tatatgcgtt ttttgtttga cgctcataat gtgtgcttaa ctttctcttg attccttcat
     2821 cgcacgaaca atagctaaaa tagcaggaat tgcagagatc actgcaagaa taattcccat
     2881 tttccctgaa ctttttcttt ttggcatatc ttctcgctcc cttctgtctt tatttatatt
     2941 ataagggagt tcttgcgaat tctcaaaaat actgtttaag aaaccaattc agcaggtgcg
     3001 ctaatatcac agaattttac gagcatgcga tacttgcttt cattgataag ttgatagtct
     3061 cgttggtcat aactttcaac atacacgaca gccgtgttga tatatgtacg ttcaatacag
     3121 ccgattagtt catgagatac tccgatcggt ttgcatatat aagatcttcc tacttctgct
     3181 tttaccattt cattccctct tttctttatt tttattttgt tttttcttta ttttataata
     3241 acaaattacc ttgtatttga aaacgttttt atccattttt ttatttttta ttttgagtat
     3301 ctttctgctt tttagataac tattttttaa cacgatctta gacgttgtga caaaaacgtt
     3361 tgtttatatt cctatgttgt tcgtgcgttt ttttctttat attgctaagt tatttataaa
     3421 gaggtgagca tatgatttat attcaagcaa tagtatttgt aaccttgatg attgggctac
     3481 ttatcggatt aaaagaaaat agctaaacca tttaagaaaa aataaataag gaaaatgata
     3541 aatgcttttt aattgcatca aaaaacgtac gatgctaaac ttggtataag ttcgaaagtt
     3601 taaaggaaac tttcaagaac taaattacat tacaaaggag atgaatttta tgacgtttac
     3661 aaaaacaaat atgcaatacc gcgtagcttt tgatacaact tctaaccgtt ttatggcgat
     3721 tgatgcacaa aacgaaaata acgttgcgtt tggtattaca atcgaacaag cagtccgagc
     3781 attaaacgaa tctagataag gagtgaacca ttccaaatgg cgactgatga gatctcggaa
     3841 aatcaataaa agacgtttgt aacggtaatc cgcgacaaac gtctttttgt attcattaca
     3901 tatatttgga aattgtctct ctttatctct agacctaaac aaaaacacaa gttctaatat
     3961 caaccatctc gttttttatg acaattttgt tagctgtttc gatgataata ttccgttatt
     4021 atctgtataa tatttaatat a
//
LOCUS       DACDPS010000138         4011 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.139, whole genome shotgun sequence.
ACCESSION   DACDPS010000138 DACDPS010000000
VERSION     DACDPS010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.139"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            44..1003
                     /locus_tag="IWJ07_002628"
     CDS             44..1003
                     /locus_tag="IWJ07_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9334148.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
     gene            1188..1625
                     /locus_tag="IWJ07_002629"
                     /pseudo
     CDS             1188..1625
                     /locus_tag="IWJ07_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318498.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(1702..3249)
                     /locus_tag="IWJ07_002630"
     CDS             complement(1702..3249)
                     /locus_tag="IWJ07_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP9334149.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(3351..3704)
                     /gene="tnpB"
                     /locus_tag="IWJ07_002631"
     CDS             complement(3351..3704)
                     /gene="tnpB"
                     /locus_tag="IWJ07_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAP9334150.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(3694..3888)
                     /locus_tag="IWJ07_002632"
     CDS             complement(3694..3888)
                     /locus_tag="IWJ07_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334151.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT         1256 a          794 c          734 g         1227 t
ORIGIN      
        1 ctcaaaatag ttccgacgaa agaattataa ggagtgagta caaatgacct ataaccatct
       61 tacaccaact gaactcgtaa tgatagaagc ttatttcaat caatcacaac ctgtttcaaa
      121 agtagccaat ctgttacaac gttctcgaca aactatttat aaggtctatc gttttcttaa
      181 atctggtgga agcgcaatga gctactataa acaatataag aaaaacaaac gtaactgcgg
      241 aagacatccg attgttttac ctgatgagca acaggaatac attcaaaaaa aggttgttca
      301 agggtgggct cctgatgtcc ttattggtcg agcagagttt cctattgctt gttcggtacg
      361 cacactgtat cgaatgttta aagataaaac ctttgacact cacgatttac caatgaaggg
      421 aaaaagaaaa ccaaacggtc ataaagaaaa aagaggaaaa caagggtttc gccggtcaat
      481 ccgagacaga aagacagact atacccagtt tgatcatgaa tttggtcacc tggaaggtga
      541 cactatcgtg ggtggaaaac ataaaagcgc ggtcatcaca ttagttgaac ggctatctaa
      601 agtcatcatt accttgaaac ccgaaggcag acaggctaaa gacattgaaa ataggctgaa
      661 tcaatggttt cgttgtgtcc caaaaaactt atttaaatcc atcacatttg attgtggaaa
      721 agaattctca aattggaaaa agattagtaa taccaacgat atttcaattt attttgcgga
      781 tccaggaaca ccttctcaac gaggattgaa tgaacattca aatggcttat tacgtaaaga
      841 tggtttgtat aaagctatgg attttaatgg tgtatctgaa acctttatcc aatcggtggc
      901 atcgaaacga aatcatattc ctagaaaatc attacactat cggacaccaa tggaagtatt
      961 tttgagttac gtcacaaaag atgatttgtc taacttaatt tgacaaacga aattacaaaa
     1021 aatattgcca tttaaaaaga aaagtgacac atactatttc ctccatcatt agtttttgtc
     1081 tatataaggt ataccctaac tagacacgac cacaacattg aaaaatctgg ttttatcgtg
     1141 tatgattagg gtgtatttta aatagttgca gaaagtaggt aaaaattttg agagttgcat
     1201 acatacgtgt ctcatccatc gatcaaaatg aacaacgcca gattgaagaa atgaaaaaat
     1261 tcggtgcagt gagaattttc atcgagaaac aatcgggcgc aacgattact catcgacctg
     1321 ttttccaaga agcattggat ttcgtccgag atcaagacat ttttattgtg gaagctattg
     1381 atcgacttgg aagaaattac gacgagatca tcgcttcagt caattaccta aagaagaaaa
     1441 acgtacagct aatcatcacc agtctaccga tcatggctga agcaattggc aaccctctcc
     1501 tggataaatt cattaaggat ctgatcatcc aaattttggc catgatcgct gaacaagagc
     1561 gtaccgaatc taaacgacgc caggcgcaag gcattaaaat tgcgaaagca aatggagttt
     1621 ataaagtaag cgccccatag aacagtacct gtacgtcaaa taacccgacc tatagaatag
     1681 aatcgggaat gtttggcgtt tttagttacg agaaaaatgt tgttgtacat ccttgttcca
     1741 aggcaacagt tgatctaaag tttctgctga aagtacggga agatttggta tcttatcaaa
     1801 tagaaactct agatacttta caggattcaa tccgttaagt tctgctgttt tatatacact
     1861 cagaatgatt cctgaagttc gtgctccttc aagacttgtg gaatgcaacc aatttttccg
     1921 tcctatcacc agctctttga tatttcgctc cgcacgatta tttgtgagtt ctaaacgacc
     1981 atcttcaaaa ataggttcaa aaccacttcg tagtttacac gcatattgaa cggctgcatc
     2041 caacttggat tttgcaacag gatctatcgt tgccatccac tcataaaaag caataaataa
     2101 tggacgcagt tcttgttgcc gacagcttaa tcgcttctca ggagaaaagt ctttccattt
     2161 cttttctagg acaaaccatt tatccaattg tttgactgct tgagctgcgg gaatatccgt
     2221 gtttttcttt ccttttggaa ttgcttcgaa aaatttccgc cgaacatgcg cgaaacaata
     2281 gacaagtcgt acactttcca cggcattata cccgctatag ccgtcacagt gtaaataccc
     2341 tgtaaactcc ttgagaaaag ttttcggaac atctccagca cgactttcgt cgtggtgata
     2401 ataaacgatg ggatgctgac tgtcttttcc tgtacaaaac tgccagtaat aggtatctgt
     2461 tttggcactt tcaatcacac gataacttgt ttcatccccg tgtagaatct cttcttgatt
     2521 aagatagact cttaatcgtt ctcctaaagg gtctaacgcg ttttgcactg ctttaatatg
     2581 ccaattggtg atattatctc tcgaaatatc caacccatgt gtttgtttcc aatagttctc
     2641 ttgccgatag gcaggaactt tctgaccaaa tttcatccgg atcgtttccg ccactaaact
     2701 attggaacct aagctattag aaatcaaagg tttaggaacg atcgcttttt taaaagaaga
     2761 aagtccttct ttttcgcagc ttttacaaca ataggtatgt tgataatggg tgagacaatc
     2821 caacatagcc tgatggaaac gaatttcttc tcgtactttt gtccgtccga tatctttcat
     2881 ctgtgaaccg caatgttcac aaaattgttc ttccaaagga aggagatgtt ctttctctgt
     2941 tataggtaag tgtttgattt tgtctgcttt cgttcctaca actcgttgat gtccttttac
     3001 tggaataatg gtggcagctg cttctggaac acttacagaa atttcttcat cactaaaaag
     3061 attcaattgc ccattcgttt cctcaaagag agtctcttta gagcgtccaa ataatttgtt
     3121 ggttaaataa ttgacctttt cattcaatag ttggatttca gttgtaagat tctgaatggt
     3181 ttgttgttgt gcttgattgg ttttatgggc ttcttgaagc aactgaagca acgtatctgt
     3241 agtttccaaa atttttcacc accttgaatc gaactgattt tgttacagaa taccacaaga
     3301 aacgcacaag aaataggggt tcaacacatt ttttgtgtta accccccagc ttaaaaaact
     3361 cctttttttc ctggcaaaat agctcgaggt tgatcgatac ttaatccttc taacaaccat
     3421 cgaacctgtt ggttcgttaa ggttcgaact tcgtctttcg tccgaggcca ttttaacttg
     3481 ccgttttcta tccgcttata acagagaaga aaaccatcac cgtcccaata gagaagtttg
     3541 aaacggtctt gacggttccc gcaaaataaa aaaacggcat cttcgtaaag ctcgagttga
     3601 tactcttgtt gaataagact ggcaagacca tcaattcctt ttcgcaaatc tgtttttccg
     3661 cacacaatat aaatatgttt ggtttgaagt aaatcataaa gcattggaca acagttcttt
     3721 caaaatagct tgaagaatat aattgttaat accgttatag atcaaaactt cagaaccatt
     3781 ctctcttttg atgcgcatcg ctaacttggc agccgcgggt ttaccttttt ctggtgcact
     3841 cttttcgttt cctaattgga caggaacaat ctcatttaat ttatacatag aaaacctcca
     3901 gacttcttga tagctcaagt atatcttgag gtgcatttgt tagtaaggta ctgttctatg
     3961 gggcgcttac tttataaagg tcgtccaaaa ctctatagtg cagatacaaa a
//
LOCUS       DACDPS010000139         3997 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.140, whole genome shotgun sequence.
ACCESSION   DACDPS010000139 DACDPS010000000
VERSION     DACDPS010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.140"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            289..696
                     /locus_tag="IWJ07_002633"
     CDS             289..696
                     /locus_tag="IWJ07_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334152.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            710..1111
                     /locus_tag="IWJ07_002634"
     CDS             710..1111
                     /locus_tag="IWJ07_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334153.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1113..1484
                     /locus_tag="IWJ07_002635"
     CDS             1113..1484
                     /locus_tag="IWJ07_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334154.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1520..1822)
                     /locus_tag="IWJ07_002636"
     CDS             complement(1520..1822)
                     /locus_tag="IWJ07_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334155.1"
                     /translation="MLMEGKTQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(2071..2271)
                     /locus_tag="IWJ07_002637"
     CDS             complement(2071..2271)
                     /locus_tag="IWJ07_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9334156.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(2576..3808)
                     /locus_tag="IWJ07_002638"
     CDS             complement(2576..3808)
                     /locus_tag="IWJ07_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP9334157.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
BASE COUNT         1330 a          663 c          702 g         1302 t
ORIGIN      
        1 tttgagggga agtacatata gttgttgata tagttgctaa tattgttagc taatttgtta
       61 gtaaaactgt atacgaattt agatagtaaa aaaacagttt gttccttata tagaggcact
      121 tttactactg attaaatgtc agatatgctt acaaatatac ttataaatat gcttacaaaa
      181 ttagctaata tttccgttga caaagctttg ttactattgt taaactaaat tgactagcaa
      241 aagtgaaaca taaatttatt aaataaatgt aaaaattagg agaaatcaat gtcttataga
      301 gtacaattaa taatttcaga agatgtagaa agtcagcaat ttggcactaa tgttattaat
      361 aaagtaatta atccagctct ttctattaat gcgcctttaa ttcctactgc tttatcattt
      421 tctgtaacag ctgtggtgtc tgagatagag gatacagaaa agataaaaat agtagaaatt
      481 gaagttttaa acaagaatga aaaacaaatt ttttctacgg gtgaagtatc ggtaagcttg
      541 cctcctcaag ttaacgatat aaactttaat ataaacgcca gaaatgtctt ggttgaagaa
      601 gcaggagagc attatgctgt tgttaaattt aatggaactg agattggaag gcaaatcttt
      661 gatatcaagg tcaacaaacc agtggaaaaa aattaaggag acgatacgga tgcttgatat
      721 agtaccatca aatacacatc ggagtggtag cttaataaat tttgctgcga tttttgcttg
      781 cgcatcggta attctagcat ctccagttgt tgtggatgca agttctactc cgaaaactaa
      841 aaatgataat caacttgtaa ttactacaaa aaatgagatt aacacaattt caagtaatga
      901 tggcaaaatt tttgatgtga taaatacggt tttaaaaaga aaagctgaaa tcaatataaa
      961 atatgatgaa gatttaaact tgtatttctt tgttatcaaa acaacatctg aattgttttc
     1021 ctcagattac gatgttttgg atactctcga taatgtatta tctgactata aatatatggg
     1081 gaaatctgta gtagcaactt tgggggaata aaatgtttga ctttaatgaa tatttatcta
     1141 tttccaaaca aattgaatca gatgataaat ataattctaa agaatcttat agaagaactg
     1201 ctgtttcaag agcatactac agtgctttta aaaaatctga tgagtatttg aaagaaaatt
     1261 atgatattat ttataatgga agtagcggaa aaggaagcca ccaaaccgtt tggaatttgt
     1321 tttcaacagt taaggaattg aataccttag gaatacataa tagtgggtat agaatgttag
     1381 agaaaagaaa atgtgctgac tatattccta atgaaaccat tacgaaaaca gatatggcgc
     1441 tgatgaatcg agaagcagaa aaaataataa acaaactcac ctaatgaatt tcaagcgagt
     1501 ttgtttgtta tttttttatt taatctttgt atctttctgg atcaacgaaa gtatacttta
     1561 tatagtcata acgccgatga tcgctacgtg cgtccggcac gtcagtcacg atatcaaaca
     1621 aaaagtatac acccttcttc attctagttt tcgcagcagg aattttgaaa tagttcttat
     1681 tagaatagta gagattgatt aataagctat cttcgattgc taaaaagaaa acttctgaat
     1741 tccatacttt ataaaaatct ttgataaatc tattcgaagg gtcaaattta aaccataatt
     1801 gtgtttttcc ttccatcagc ataaaagttc acctcaaaaa gagtatacga acgaatgttc
     1861 tttttgtgaa ggcggaattt aatatactga ataatagaaa acaagtactt gtgccaaatt
     1921 atgtgccaaa aaaattcgaa ttcaacaaaa tgtaaacaaa tagaaaacct tctatattaa
     1981 gatttcctgc aataacaaac acaacagaag acgtgtaata gttagtcagg aacgtacaaa
     2041 taacccctgt atcctttgcg gtacaggggg ttattttgta taagtagaca ttgtagtttc
     2101 taaactgcta tgtcctaaac gtttcgatac gctaagtata tttactcctt gataaagtaa
     2161 aatagatgca tgcgtatgtc ttagtccatc aataaacagt cgaagagaaa atagggaaaa
     2221 attgattact aaagaaaaac ttactaggtc gacggtgtag ttgattttca tacttcaata
     2281 gattcgaatt aaacgatttc gattggctta taccagtcct atccaacgaa agatttatcg
     2341 ttcttaactc tagcggtata acttgaagct ttacgtaaag ccaatgggaa tgaactggag
     2401 atgacttttt attattttta gtaatagctg atgggttaat acttacaact ttccttttca
     2461 aagtaccata taaaaaaacg gtggggttgt ttagcgaaat aaaggatcta agccaatagc
     2521 ttagatcctt tattctatgc tttatattat tttttgcatg tgtgcgaaag tatactcaag
     2581 cccaatcgcc atttcggaaa atcggtactg tagagccatc ttcacggatt ccgtcgatat
     2641 ccatcttgtc agatcctacc atgaaatcta cgtgggtttg acttctattc aatccagctt
     2701 ctgctaattc ttcatcggac atttctgtac cgcctttcac actgaaggca taagcagaac
     2761 ctagtgctaa gtgattcgat gcgttttcat caaataatgt attgaaaaag atgatgccag
     2821 attgagaaat aggtgaaggg tcaggtacaa gtgcgacttc tcctaaacga cgcgcacctt
     2881 catctgtatc aagcagtttt gctaagacat cttctccttg ttcggcagaa aaatcaacaa
     2941 cttttccatc tttaaaagta aatttcatac ccgagatgat ggtgccagca taactcagtg
     3001 gctttgtact tgaaatgtaa ccatctactc gatgactgtc aggtgctgtg aacacttctt
     3061 ccgtcggcat atttgccatg aatttttctc cacgagcatt atagcttcca gcgccttccc
     3121 aaagatggtt tttaggcaat ccgataatga tatctgtacc gggagcagta tagtgaaggg
     3181 cagaaaattg ttctcgattc aattcttctg ctttttttgc taatttttca tcatgcttct
     3241 tccatgctaa gacagggtct tcttcataga cacgagtcgt tttgaaaatc tgatcccata
     3301 atgcctcaac ttgtttttct tctggcagtt ctgggaagac ttttgctgcc cattgtttac
     3361 cagccgcagc cacgacagtc caactgactt tatttgcttg agttgccttg cgtaaattca
     3421 ttagtgcttt gccattcgct gattgataag acgctactcg atggctatct acaccagcaa
     3481 atgcatcggg atcggcagaa acaacgctga tcctgcttgc tcctttttca agccattcat
     3541 ccgcttgatc gattttagat tgtggtacat tctcaatacg atccgttgca gcatgtaaaa
     3601 gaaattctct ttggatctgg tcgtcagtcc attgaacgat aacctcagcg gctcctagtt
     3661 tataagcttc ttgtgtgatc aatcgagcaa ggggtgcctg ttccacgctg atctgtaaaa
     3721 caactgtgtg tcctttttct gtggctacgc cagtttctgc aattaagcgt gcatattttt
     3781 ttagaagttc attaaaatca gataacataa taaaaattcc tcctcaccat tttttctggt
     3841 aactattagc atgataccat ttaattatga ttcaaacaat gaggctttaa ataaaaaaag
     3901 gaggttattt ttttaaggtt aagtttcatg atatatgtta ctacttgcta tttcagcttg
     3961 tgaagaaaat caaaaactaa ttttgcatat caaaaaa
//
LOCUS       DACDPS010000140         3906 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.142, whole genome shotgun sequence.
ACCESSION   DACDPS010000140 DACDPS010000000
VERSION     DACDPS010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.142"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            90..1511
                     /gene="gndA"
                     /locus_tag="IWJ07_002639"
     CDS             90..1511
                     /gene="gndA"
                     /locus_tag="IWJ07_002639"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAP9334158.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            1704..2390
                     /locus_tag="IWJ07_002640"
     CDS             1704..2390
                     /locus_tag="IWJ07_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9334159.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTVLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            2387..3892
                     /locus_tag="IWJ07_002641"
     CDS             2387..3892
                     /locus_tag="IWJ07_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9334160.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
BASE COUNT         1321 a          676 c          835 g         1074 t
ORIGIN      
        1 aaatatgttg ttctttagtt tgcaatgtag ccgtgttaag ctataatgta agagattagg
       61 gagaaatccc gtatgtagga ggagttcata tgtcaaaaca acaaatcggc gttgtcggta
      121 tggccgttat gggtaaaaat ctagccttga acatagaaag ccgcggctat tcagtggctt
      181 tattcaatcg tacaggggct aaaacaaccg ctgtagtaga agaacaccca gataaaaatt
      241 tcaaagcaac atatacaatc gaagaatttg tagaatctat cgagaaacca cgtcggatct
      301 tgctaatggt caaagcagga ccggcaacag atgcaacaat ccaagagtta cttcctcacc
      361 ttgataaagg cgatattttg attgacggag gaaatacatt ctttaaagat acgatgcgtc
      421 gtaatgaaga gttagccaac tctggcatca actttatcgg tacaggcgtt tccggtggtg
      481 aagaaggagc tttaaaagga ccttcaatca tgcctggtgg acaaaaagaa gcctatgcgc
      541 tagttgcacc aatcttagag caaatttctg caaaagcaga agacggagca ccatgtgtca
      601 catatattgg cccaaatgga gccggacatt atgtgaaaat ggtacacaac ggtattgagt
      661 atggagatat gcaactgatt gctgaatctt atgacttaat gaagaatatt ttaaaccttt
      721 cagtagaaga aatggctgat atctttaaag aatggaatca aggcgaatta gacagctatt
      781 tgatagaaat cactgctgac attctgacac gtaaagacga tgaaggaaca ggtaaaccag
      841 tagtagatgt gatccttgat gcagctggga ataaaggaac aggtaaatgg acaagccaaa
      901 gcgcgcttga cttaggtgtg ccacttccat tgatcactga atctgtattt gcccgctata
      961 tttctgctta taaagaagaa cgcgtacaag caagcaagat tttgtctcgt acgaacgatt
     1021 ttgagttcac gggagacaaa aaagaattgg tcgaaaaaat ccgagaagcg ttgtatttca
     1081 gtaaaatcat gagttatgcc caaggatttg ctcaattacg tgtcgcttca aaagaatttg
     1141 attgggactt gccatttggt gaaatcgcaa aaatctggcg cgctggatgt atcatccgag
     1201 ctcgattctt gcaaaaaatt acggatgcct atgacaaaaa tcctgagatc gaaaatttat
     1261 tgctagatga ttacttcgtt gaaatcacga aaaaatatca gcaatcagtc agagacgtcg
     1321 ttgccttggc tgtccaagct ggcgtacctg taccgacttt ctcatcagcc atcgcttact
     1381 tcgattcata cagagcagaa cgtctgcctg caaatataat ccaagcccaa cgcgattact
     1441 ttggcgcaca tacctatgag cgtgtcgaca aagaaggaat cttccattac tcatggtacc
     1501 atgaagaata atagtctttc ggctgttagg aaatgagaaa ataaaaagag gacgaaagag
     1561 ctgaagtcga ttgttatgag tagacttcag ctctttttta aaattgctat ccatgataaa
     1621 atgactatag atttttaagc atattatcgg tataatgata gagtttttaa aaagccagta
     1681 gaatagaaag gataaaataa taaatgagca atattctaat cattgaagat gaaaagaact
     1741 tggcacgatt tgtcgagcta gagttgaagc atgaagggta taacgcagaa gtccactaca
     1801 acggccgaac tgttttagat gcggcattaa ataatgagtg ggatgccata ttgttggatc
     1861 tgatgctccc agaattgaac ggtttagaag tctgcagacg tgtacgccaa gtcaaaaata
     1921 caccaatcat tatgatgact gcaagagatt ctgtgatcga tcgtgtatct ggacttgacc
     1981 acggagcaga tgactacatc gtcaaaccat ttgctatcga agaattatta gcacgtcttc
     2041 gtgcattgtt gcgtcgaatc gatattgaag gcgataaaaa tgtggctaaa caaactacta
     2101 ttacttatcg cgatttgact atcgaaaaag aaaaccgtgt cgtacgtcgc ggaaatgaag
     2161 taattgaact aacaaaaaga gaatacgagt tgctgcttac tttaatggaa aacgtgaacg
     2221 tggtattagc acgtgacgtt ttactaaata aagtgtgggg atacgaaaca gaagttgaaa
     2281 caaacgtagt ggacgtatat attcgttatc ttcgcaacaa aattgacgtt cccggggagg
     2341 aaagctatat ccaaactgta cgcggaacag gttatgtgat gcgctcgtga taaagccgaa
     2401 aaaatcggaa aaaagagaac tgaaagggcc ctccttgaca atcaagtggg cctttgcgag
     2461 ttctttcttc atattcgttg tgtttacaat tttcgctgtc attacctata aatcatctat
     2521 caatctgatt gtggcaaaag aaagaaacaa tgtagagcgt acgatttcag aagtagcttc
     2581 tcgtttggct aactcggatg aagaactgac attagtaaat acgtatcgta atctgacgaa
     2641 agcgacaaat gacggagatg aggtcgtaga tcatacttca ctggtcgaag gaaacttaat
     2701 gaagatcgat tcctttatag ctgagctagg gcagccatca atggatcttt atgtttatga
     2761 tttggacgag aacttgattt ttaaaaccca tgaaaacagt cgaaacctga ttcagactac
     2821 acgtaaagtt ccaacgatcg ttacactaga aggaaaaact ggctttttat ctatccagcc
     2881 tatatattct aaaggtacaa gagaaaagat cggctatgcg caaagcttct atgaattatc
     2941 ttctttctat gatattcgaa ataagctatt attgactctt attgttttag aagttgtttc
     3001 tttgatctta agtagtattt taggtttctt tttatcttct tatttcttaa aaccactcaa
     3061 ggtactcaga gatacaatgg atactatacg taaagatccg caatcagata tccacatgcc
     3121 tgaaatcgac acaaatgatg aattggcaga tttagctgaa atattcaatg agatgcttga
     3181 ccgtatgcgt ttgtatattg aacaacaaga gcaattcgtg gaagacgtat ctcatgagtt
     3241 aaggacacct gttgcaatta ttgaagggca tcttagtctg ctaaatcgtt gggggaaaga
     3301 tgatcctgag atattagaag aatcattaag cgccagcatg caagaaatca gccgtatgaa
     3361 aagcttagtt caagagatgc tagacttatc aagagcagaa caagtagatg ttcattatgc
     3421 gaatgaaaca actaaaggaa aagaagtcac ctatcaagtt tataataatt tcaagatatt
     3481 atatccagac tatgtgatca ctttagatga tgatttgaca aaagaagtga ccctgcaaat
     3541 ttatcgtaat cactttgaac aattaatcat tatcatattg gataacgcag taaagtattc
     3601 aactacacga aaagaagtcc atatctctat ttcttccact ctgaatgaat ttgagatcgc
     3661 gattcaggac tttggcgaag gaatacccaa agaagacctt aataagatct tcaatcgttt
     3721 ttatcgagtg gataaagcaa gagctagaac gaagggtgga aatggtctgg ggttgtctat
     3781 cgctaaacaa ctggttgaaa actataaagg acgtatctta gcagaaagtg tagtagggca
     3841 agggacaatt tttagaatct ttattcctat cgcaaaggca gaagacaagt aaagcatgtg
     3901 ctttac
//
LOCUS       DACDPS010000141         3778 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.143, whole genome shotgun sequence.
ACCESSION   DACDPS010000141 DACDPS010000000
VERSION     DACDPS010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.143"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..237
                     /locus_tag="IWJ07_002642"
     CDS             <1..237
                     /locus_tag="IWJ07_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP9334161.1"
                     /translation="PRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVK
                     GLESRFESILLAYSVLLSRAQRRFGGTLRYSLGY"
     gene            complement(240..488)
                     /locus_tag="IWJ07_002643"
     CDS             complement(240..488)
                     /locus_tag="IWJ07_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin acetyltransferase"
                     /protein_id="HAP9334162.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLLTRGAS"
     gene            complement(485..1393)
                     /locus_tag="IWJ07_002644"
     CDS             complement(485..1393)
                     /locus_tag="IWJ07_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAP9334163.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            complement(1426..2160)
                     /locus_tag="IWJ07_002645"
     CDS             complement(1426..2160)
                     /locus_tag="IWJ07_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9334164.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            complement(2141..3010)
                     /locus_tag="IWJ07_002646"
     CDS             complement(2141..3010)
                     /locus_tag="IWJ07_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAP9334165.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARSTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            complement(3025..3156)
                     /locus_tag="IWJ07_002647"
                     /pseudo
     CDS             complement(3025..3156)
                     /locus_tag="IWJ07_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            3227..3439
                     /locus_tag="IWJ07_002648"
     CDS             3227..3439
                     /locus_tag="IWJ07_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334166.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            3601..>3778
                     /locus_tag="IWJ07_002649"
     CDS             3601..>3778
                     /locus_tag="IWJ07_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334167.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSR"
BASE COUNT         1145 a          778 c          568 g         1287 t
ORIGIN      
        1 ccacgtaaaa acatggataa atcagaaaaa ctagaccaca gcttacttgg aaaacaaaga
       61 aaaacagttg agactgtttt ttcttcctta gaaaaattag gttgtcaaaa tttcaactca
      121 cgttctgtta aagggctaga gagtagattt gaaagcatat tactggctta cagtgttcta
      181 ttaagtcgag cacaacgacg ttttggagga actttgagat attctttagg atattaaaat
      241 taactagcac cacgggttaa aagatatatt atcttatcag tattgtcaat atattcaagg
      301 caatctgcct cctcatcctc ttcatcctct tcgtcttggt agctttttaa atatggcgct
      361 tcatagagta attctgtaaa ggtccaattc tcgttttcat acctcggtat aatcttacct
      421 atcacctcaa atggttcgct gggtttatcg atatctttca ggtgccctgc tttcatttct
      481 gtaatcactg ttcccgcctc tcttctatat cagcggcata tgtgctatcc aggcagccgg
      541 ttttaccagt gtatttttta tacatgtccc tggtatattt tgttatattc ctatcatact
      601 ccggataggc ataatgaagc ctttccgcca cctcaccgga taccgccctg aacaattgat
      661 ggcatagaaa taatgcttcc catatgtttt cataggaatc catccggtag gtggacaaaa
      721 gtttctccca caaatcctcg gatatatacc tttcaataaa cttatagttc ttgcctacac
      781 ttaatttaaa gcctgtttcg atgccgacct tccatgatat cattctcagc agctcatggc
      841 gaacaatctg attaaaatga tcaatagcaa ataaaatttc cttacggcac aatcctttaa
      901 taacataagg tgttacattc caaaattcat tgcagcaatc atcatactcc cttgcgcttg
      961 gctttcttac atgataatct atatcagtcg gaactatgtc ccttttaatt ctacaatctt
     1021 tatcaattag aacctttatt aatttatcgc cctttaggta attatctaac tcttccaagg
     1081 gcaataaggt aagatcaatt ttattgtaat catcaaatag cataagatag gaaaatccct
     1141 tttcttcagg tgggaataat tccatatcct ccggcttttg catcattatt atattcccaa
     1201 attgattaag ccagtcatca ttagatataa acggttctat atcacttaca aaatatgtaa
     1261 tatcataatc ctgaaattca tctttaggta tattaatatt tgcgcgtgac ccctcaaggg
     1321 tcacaattcg aatacgttca tcctgttctg ctaaagaaag tactaaatcc atcatttctt
     1381 tttctgatct catttacata ctcctcgttt attttttcta tattattaca tcttcttttt
     1441 tgccgataca atcagcatca ttgggcgtcg catttcatcc gccatccccg gaatatccat
     1501 catgttctct ggcggctgtg gctccacaat ctgatttatt ataaaactat ttgaaagcag
     1561 tgtatttaga tatgtggtca gtgttctatg atattttgta accttttctt ccaaaaacat
     1621 agctgtccgt ttgccctcat aataataatt gtccaccggg aaatgcagta tttctccttt
     1681 ttcgttataa taccagtctt gtgttccatg agcagtaaaa acaggatgtt caactgtaaa
     1741 aactaaattg ccaccagcct tcagcatcct atatatcttt tttattaaat tctcatagtc
     1801 tgctacataa tgaaacgcaa gcgaacttag tattacatca aagctctcct ctgggaaatc
     1861 cacatcttct atggcacagc attcatattc aatctgtgga aaatgggttt ttccttttgc
     1921 tacttcgagc attttatgag aaatatcaac acctactaca gaggaagcac cgttttccat
     1981 cgcatatata cagtgccatc catagccgca tcctaaatca agcacacgct tacccttaaa
     2041 atcaggtagc atctttttca aagtctccca ttctcccgca ccagccagtc ctttctgcga
     2101 gcgactcatt tgactgtatt tttgaaaaaa tatattatca tcatatttgt tttctttcat
     2161 ctgaactctc ctcgtttaaa aagttattta tctccgatac aatttcattc acttctttat
     2221 aaagtttctc ggtcatgtcg tagcattcaa aaagagaaag accgagtact tcaaaaatat
     2281 gattaactct ttcggcatat ttttcaggtt tatattcaaa agtttcaagc agttttatag
     2341 ctttcttttc gttaatgcaa taagcattat tacatgcaaa tagtacttga tttaagcatg
     2401 aaataatacg aaaaacatgg cctgcaatat aatatttatc ctctgccccc gcatttgctt
     2461 ttacaaacat taaagagaat tcagcttcaa atataaaaaa gttcatcaag cttttcttta
     2521 gagcagtagg gtaaatttct gcctgctttt ttaattcgca taagctttcg ttcttagcat
     2581 atagtatttt actaatcgct aattctccac gatacatagc acttatataa ccatggggat
     2641 gcccggtctg ataattggca gtaacaattc cttgctccgt atctttgatt atttgttcca
     2701 cccgttttat atcacgtaat atcaagtcaa catgacaccc gtttataact aaccatccac
     2761 cgccattaac ccaatcaccc cacgctccgg gaggtacaac aaggttgttt ctattctcat
     2821 catccaactc tgtagcaatt tgattaatcg ctgtcaggtc aaatgagtct gaattgtaat
     2881 agattccgat atctatatca gaattttctg tatgggtgct tcttgcacgt gagcccccta
     2941 atacaatgcc ttctataaaa ggcaaagatg ataatttctc tgttactatt tgaataacat
     3001 tacctaccat ataaacatcc tcctttattc gttagatatt tttatttcat ctccatctgg
     3061 taaaataaat gcctgcacaa acttgacacc tagcacatca gcaatggcct tcaactcatc
     3121 caaggttact gtttctcgtt gtaatttttt attaaaaaat ctaattagct tttttagtca
     3181 atctaaagtt ctttattcta tgatataatg ttactgtaga aagtttgtgg cggtttggca
     3241 aatccaattg aaggagcgtg gtgcctatgg gcttattagc ttatcggacg tactctgtag
     3301 aaaggaaatg cctgatttgt tctcattgga tcaaatcatt gctattatcg gagcagcagc
     3361 agtagttatt actgccgtag cgaaagctag cgattcgatt ttagcttggt taaagttcaa
     3421 aaaagaacaa aaaaaataga gccgtctcaa cttttggcga ggtagacgag ctctaaagct
     3481 ttatttacat gccatccgct ttgaacggac tacactgggc atgtgacagc atgccctttc
     3541 atttatatta tatctcattc ttttatataa tgaaagtaaa taaatcatgg aggataaaat
     3601 atggataaat ttaatagatt aacattaata aatcagtttg aaattttaaa agctttaaat
     3661 cctaacgaag aaaaatacta tgctgaaaaa atagaaatac taacagaagg ttacgaatat
     3721 cactatagtg aaatatttga aaatatttct gatccattgc cagaagaaga ttcgcggt
//
LOCUS       DACDPS010000142         3747 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.144, whole genome shotgun sequence.
ACCESSION   DACDPS010000142 DACDPS010000000
VERSION     DACDPS010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.144"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(54..512)
                     /locus_tag="IWJ07_002650"
     CDS             complement(54..512)
                     /locus_tag="IWJ07_002650"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAP9334168.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(683..1828)
                     /locus_tag="IWJ07_002651"
     CDS             complement(683..1828)
                     /locus_tag="IWJ07_002651"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAP9334169.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(1841..2758)
                     /locus_tag="IWJ07_002652"
     CDS             complement(1841..2758)
                     /locus_tag="IWJ07_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAP9334170.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3186..>3747
                     /gene="efp"
                     /locus_tag="IWJ07_002653"
     CDS             3186..>3747
                     /gene="efp"
                     /locus_tag="IWJ07_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAP9334171.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT         1148 a          722 c          722 g         1155 t
ORIGIN      
        1 aatacgaaga taagatagct atattgcttc tcatctttac gttcttttag aaactatgct
       61 tctacttcag accattcatc gcgttttgct aaaaattcac gagccaaagc ttgtgcacct
      121 tctagtgtat ggctggcagc ccagccacat tgtgtttcat tgcttgcagg tacttcagta
      181 gcttcaagaa catctttcat tgttgcttca agaacatcta aaatgtcttc gtagctttca
      241 tgattcaata cagtcaagta aaagcctgtt tggcatccca ttggagacca gtctacgatg
      301 taatctgcgt gattgcggat tagttcggca gtcaagtgct caagtgaatg tagacttggc
      361 atgtccatgt gttctttatt tggttgttta aagcggacgt cgtatttaat gatgacatcg
      421 ccatgcgcac ccgtttttcg atcagctaca cgtacataag gtgctttgac ttttgtatgg
      481 tcaagattaa aactttcgac attcattctc atttgtaaga ctcctatctg tttgttcatt
      541 cgttttgatg cttttcacta aagaattatt tagtgaaatt tacaaaaacg tctttctact
      601 tgattctaac acttatcgtt tccaaggtgt agcaatctga aatatttttt agatttttct
      661 gctaagtcgt ttattgggac ttctagttct tgattctgtc aaatgcctga gctaaatcgt
      721 ttatgagatc ctgaccatac tcaattccta cagaaagacg gatcaagccg tcctttatgc
      781 cagcctcttc tcgtttttct tttggaatag acgcatgagt catgacagaa ggaatttcca
      841 ctaaactttc tacgccacct aaactttctg ctaaagtaaa tagttggagt gattcaacaa
      901 atggaatagc agattcttca ttttttaaag taaaagaaat cattccgcta aagccgctca
      961 tctgctgctt agcaactcca tggagttcat gatcgggtaa tcctggatag tagacctttt
     1021 ctactgctgg atgagaaaat agaaaatcag ctaccacaaa agcatttttt tgatgttcct
     1081 ccatacgaac acttagtgtc ttgattccgc gctgaagtaa ccagctatct tggggaccaa
     1141 gtacggctcc gatagcattt tggtaaaatc cgatctgatc ggctagatct ttatggttag
     1201 aagtcactaa accagctact acatcgctgt gtccgcctag atatttggta ccgctatgga
     1261 caacgatatc agaaccgaga tctaaaggtc tttgaaaata aggagtagca aatgtattgt
     1321 cagcaataac tatcaatcca tgttgatgag ccagagtggc acttttttct aaatcagtaa
     1381 tctttagaag cggattgcta ggtgtttcaa gataaagtgc ttttgtattg ggtttgatgg
     1441 attgttctat tttatcaaga ttactcgtat ctatgatcgt gtactcgaga ccatttttag
     1501 taagaacttt gtcaaataga cggaatgttc ctccatagac atcatctcct aataagatat
     1561 gatcgccagc ttgaaaaaga gaaaaaacgg catgaatccc agaaagacca gaagaaaaag
     1621 cgaatcctcg aacaccgcct tccaaatcag caatcagttc ttccaatgca aaacgagtag
     1681 gattaccaga tcttgaatat tcatattgtt taggctggcc gacgccatct tgccgataag
     1741 tggatgtttg ataaataggt acactgacag ctcctgttgt gggatcttca ctgatgcctc
     1801 cgtggatcaa ttttgtttga atgtgcatgt ttctttcctc ctataagtaa atgttttttg
     1861 ataaatagcg gtctgctgcg tctggaaaaa tcgtaacgac acgatgacca ggtggtaaac
     1921 gacgaatttc tcttaatgct gctgcaaaag ccgcaccact cgaacttcca actaatagtc
     1981 cttgttctcg tgctaactgc ctagtatagt taaacccttc tacatcagag attgtctcga
     2041 tctgattgat tgaaagggga gaaagaaacg gcggaatgaa ttctacaccg ataccctcaa
     2101 tttcgtgagg ggctggatca ccaccattta acacagagcc ctctggttct acaccgatta
     2161 ttcgaatgtc gggatatttt tctttcaaaa aggtagaagt tccagcaaag gttccaccac
     2221 tcccaattcc tgctacaaaa ctatggatat tttctttgat ttcatgaaag atttctggcc
     2281 ctaacgtatg atagtaagca gcaggattat ctttattttc aaattgaaga ggcagatagc
     2341 tgtttgatat ctcctctgca agttttttac ttttgctgat ggcaccgatg attccttgct
     2401 cactaggaga atggatgatc tttgctccca atgctttcat caattgttgt ttttctaagc
     2461 taaacttctc tggaacaaca aaaaccgttc ttaatttgta tgtgagtgcg gccagagcga
     2521 tcccaattcc tgtatttccg gcagttggtt cgatgatcgt cgttttttca ttgatttttc
     2581 cttgggcaaa tccttctttg atcaaataac tgcccaatct atctttgata cttcctccag
     2641 gatttaaata ttctagtttt gcataaatag cagaaccttt aggcatagaa tagtgctgat
     2701 tgtcaaagcg ataaagcggt gtggaaccaa tagcttctgt aattgtcgag ataatcatgt
     2761 gggcctccta aaaaaagata ataaaaaaag gagctgacta ttaaatcagc tccttgaaag
     2821 acatagagaa aaattgagta gataaaaaga taagttacct ggtactcatt ctatgaaggg
     2881 caattaatag acatgacaaa tagtatgctg cttttatgca ccatacaaca gcaacgattc
     2941 aattgttttt ggttgctgac tgtcatgtgt atccctccaa gtaaaatttt tattaacgat
     3001 agcatgaaaa aaaataaaag tcaaaggctt gaaagaaaaa aatatttcga tagcatcgtt
     3061 tacccgtgtt tatttttctg acaaataggc atgagacctt aaaagataga gaatactcta
     3121 gttttctggc cggttgtctg atacaataaa gttgattatg ataaagaaaa gaaggagcaa
     3181 aaataatgat ctcagcaagt gatttaagag ccggtatgac tttcgtacaa gacggcaaac
     3241 tgattaaagt tctggatgca agccaccata aacctggtaa aggaaataca gttatgcgta
     3301 tgaaactgaa agatgtccgt tcaggtgcaa catacgacac aacgttcaga ccagaagaaa
     3361 aatttgaaaa agcacatatc gacacaaaac ctgtacaata tttgtacacg atggatgata
     3421 cagctttctt catggatttg gaaacatatg agcaatatga aattcctgta gaaacagtag
     3481 aagaagaaat gaaatatatc ctagaaaaca tggaagtaaa aatccaattt ttcggtagcg
     3541 aagtcatcgg cgttcaatta ccaacaactg tcgtattacg tgttgtggaa acacaacctt
     3601 ccatcaaagg tgcaacagtc actggttcag gtaagccagc gacaatggaa actggcttag
     3661 tagtcaatgt tcctgacttc atcgaaacag atgaattgtt ggaaatcaat acagcagaag
     3721 gttcttatgt aaaacgtgcg tcaaaat
//
LOCUS       DACDPS010000143         3712 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.145, whole genome shotgun sequence.
ACCESSION   DACDPS010000143 DACDPS010000000
VERSION     DACDPS010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.145"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1309
                     /locus_tag="IWJ07_002654"
     CDS             <1..1309
                     /locus_tag="IWJ07_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP9334172.1"
                     /translation="SSFLLKFRGEIPLRNLETFQHSAIIISVIFIALNILLGAYVFYN
                     RMISDIVFVTVIGQVLMTLGIMVVTFAGRWFAFPRLVILLSFVLGTLFLSIYRILVYK
                     LYLRVSRDKKVVIIGLEKDVAPAIQNFGSKKNNKHKVEAVILDNYYANIKNMIKDIDI
                     VYLASHIEETEKIKIYQLVTKNEKKLFLNTTFENLTLINPNIMNFEDESIIEASGFRI
                     QPEYELFKRLFDIIVSLVLLVIASPFMLFTAILVKVTSPGPIIYKQVRITKNQKEFSI
                     YKFRSMTATAEAKSGPVLAKSNDARVTPVGKFIRAVRFDELPQIFNVLKGDMSIVGPR
                     PERPFFVDQFNAENPYYYLRHNVRAGITGYAQVYGKYVSDYNSKLRFDLLYIKKYSLM
                     LDLKILLQTIKILFDKMSSQGLEEDENVISLAEIAFPNEKIFR"
     gene            1325..2365
                     /locus_tag="IWJ07_002655"
     CDS             1325..2365
                     /locus_tag="IWJ07_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9334173.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            2384..3469
                     /locus_tag="IWJ07_002656"
     CDS             2384..3469
                     /locus_tag="IWJ07_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAP9334174.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            3502..>3712
                     /locus_tag="IWJ07_002657"
     CDS             3502..>3712
                     /locus_tag="IWJ07_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP9334175.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSI"
BASE COUNT         1326 a          531 c          688 g         1167 t
ORIGIN      
        1 tagtagtttc ttactgaagt ttcgtggaga gatcccatta cgaaatttag aaactttcca
       61 acactcagca attattattt cagtgatttt tattgcgttg aacattttgt tgggtgcata
      121 tgttttttat aatcgaatga tcagtgacat cgtgtttgtc acagtcatcg gtcaggtatt
      181 gatgacacta gggatcatgg tagtgacttt tgcgggccgc tggtttgctt ttccacgtct
      241 tgttatctta ctttcatttg ttctcggtac gcttttctta agtatctatc gtattctcgt
      301 atataaactt tatttacgcg taagtcgaga taaaaaggtc gtcattattg ggttagaaaa
      361 ggatgtcgca ccagcaatcc aaaattttgg ttcaaagaaa aacaacaaac ataaagtcga
      421 agcagtcatt cttgataact attatgcaaa catcaaaaat atgattaaag acatcgatat
      481 tgtctatctt gcttctcata tcgaagaaac tgaaaaaatc aaaatctatc aattagttac
      541 taaaaatgaa aaaaaattgt ttttgaatac aacatttgaa aacttgacat tgatcaatcc
      601 aaatattatg aatttcgaag atgaaagtat tattgaagca tctggattta gaatccaacc
      661 tgaatatgaa ctattcaaac gtttgtttga tatcatcgtt tcacttgttt tattagttat
      721 tgcttcacca tttatgttat tcacagcaat tttagtcaaa gtgacatcac cgggaccaat
      781 catctataaa caagtgcgga tcacaaaaaa tcaaaaggaa ttttcaatct ataaattccg
      841 ctcgatgacc gccacagccg aagcaaaatc tggaccagtt ttagctaaga gcaatgatgc
      901 acgagtaacg cctgtaggta aatttattcg agctgtccgc tttgatgaat taccacaaat
      961 cttcaatgtg ctgaaaggcg atatgtcgat cgtaggacca cgtcctgaac gtcctttctt
     1021 cgtcgatcag ttcaatgcag aaaaccctta ttactactta cggcataatg ttcgtgctgg
     1081 tatcactggg tatgcacaag tctatggaaa atatgtctct gattacaaca gtaaattacg
     1141 ttttgattta ctttatatca aaaaatattc attgatgctt gatttaaaga tcttgctgca
     1201 aaccatcaag atcctatttg ataaaatgtc ctctcaagga cttgaagaag acgaaaatgt
     1261 gatctcattg gcagaaattg cttttccgaa tgaaaaaatc tttcgctagt tgggaggcgt
     1321 aaaaatgaac aatccgaaat tcagcataat cgtaccagta tacaatgcag aagcaactat
     1381 tggtcgaacg attgatattc taaaagaatt ggattatgaa gagtacgaag tcgttttagt
     1441 aaatgatgga tcaacagatc aaacacagga aatcattcaa gaactgattg aaaatgatga
     1501 acgttttgaa ttgattacta cgaccaatca agggcccgga ttagcaagaa acgaaggaat
     1561 aagacagtca aaaggagaat atctcttatt cttcgatgca gatgacacgc cagtaaaaac
     1621 gatcttgtca gattatgatc atttgctaga aaagagtcca aaagcggatc ttgttgttag
     1681 ttcgtttgtt tttcgtacaa tggacgaggg aagaatcgtt tccgaaaaag aaaacttagt
     1741 tacagaatgt cactatctaa ctcacgaaga gtttataaaa gacatgtatc agttaatgaa
     1801 tgaacagttg atgtatgttg tatggaacaa atgctatcgt agcgatattc tgaaaaataa
     1861 ccaaattcta tttaaaactt ataatagttg tgaggatcga atatttaact tacattatta
     1921 tgaatactgt catcaagtga tcatgaaccc aaaagtagaa tatatttatg aatttgaagg
     1981 tgggaagggg atcactaatc aataccgacc taacaagttc gaaacattta aagaattcta
     2041 ttcattagca aatactgtaa cagacgatat taataaagac ggtatggcgg cgcttctttt
     2101 aaaagggaca acttcagtta ttttttctat ttatagtacc tctgcaagaa cttcaaaaga
     2161 gaagaaagac gaagcaaagc agatattgag tgatccagtg atccgagaag ccaagcaaat
     2221 tgctaagacg gattcttcag caaaaaaaat aacaaagttg ctttacaata tgccagctcc
     2281 actattttta tctgctgtaa aagttggaag cttcgttgaa gtgaaaatgc cagggttaat
     2341 ggctgctttg aagcgagtat attagttaat ggaggaaaat ataatgaaaa gaattttgca
     2401 ttttcaggga cgaatgggtt taggtggagc agagtctttc atgatgaatt tatatcgtaa
     2461 aattgatcgt acaaactatc aatttgattt tttgatttat gaagattatg cagatgttca
     2521 agactatcat tcagagattg aaagattagg aggccgtatt tttgttgtac ctaatcctaa
     2581 gaaaaatatt tttaaatatt taatagaagt gaataagtta ctaaaaaaag aatctttttc
     2641 tatagttcac aatcaggttt attttggcgg gggaattaat ttatggttag ccaaaaagaa
     2701 tggaatcaga cagagaattg ctcatagcca tgcaactgaa gatggaaaat ctcaaaatat
     2761 tgtaatgaat gtattgcgta aatttttaac caaattgttg ttacaaaatg caacagacta
     2821 ccttgccgtt agtcaagaag ctggtgaaag tctgtttcaa aaccatccct ttgagatagt
     2881 tcataatggt attgatttag agctttatag taaaaattca gaagctaagg ttaataagag
     2941 aaaagaattg gatatctcaa tgagtacttt tgttgtggga aacattggac gattagagaa
     3001 acaaaaaaat caatcttatt tgttagagat ttttttagaa atactaaaaa tgaattctga
     3061 ttcttacttg attttagtag gtgaagggtc acttagaaat aaactgaaag ccaaagcagt
     3121 agaattggat atagagaaaa gagtaaaatt tttaggagaa agaaacgata ttcccgagtt
     3181 attatctgtg atggatgttt ttgtaatgcc ttctctttat gaaggtctgc caatggttgg
     3241 attagaagca caagcagcaa aattaaaatt agtcttagca gatactattt ccaaagatac
     3301 aaagttaaca aataatgtgg attttgtatc attagatgaa acaccacaag aatgggcaaa
     3361 aaaagtaata aaagagccgt atgctaataa aataacacaa gaactaaaga gttatgatgt
     3421 ttcgtacact actgaacaaa tggagagtat atatagtaga gatcattaaa aatgttcaag
     3481 atattttagg agtgattatt tttgaaaata tgttatttga tattggcata caatgatatt
     3541 ggcaacttaa agagattggt caatcgattg aacgaaaatg cagtttttat tattcatata
     3601 gataaaaaaa ctgatataga accatttatt gaagcattta aatcatatga taatgttgaa
     3661 tttttatgta ataaaaaccg tgttaaaatt tattggggtg gtttttccat tg
//
LOCUS       DACDPS010000144         3701 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.146, whole genome shotgun sequence.
ACCESSION   DACDPS010000144 DACDPS010000000
VERSION     DACDPS010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.146"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            143..1132
                     /gene="galE"
                     /locus_tag="IWJ07_002658"
     CDS             143..1132
                     /gene="galE"
                     /locus_tag="IWJ07_002658"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAP9334176.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            1144..2628
                     /gene="galT"
                     /locus_tag="IWJ07_002659"
     CDS             1144..2628
                     /gene="galT"
                     /locus_tag="IWJ07_002659"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAP9334177.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            2652..3632
                     /locus_tag="IWJ07_002660"
     CDS             2652..3632
                     /locus_tag="IWJ07_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9334178.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
BASE COUNT         1251 a          664 c          808 g          978 t
ORIGIN      
        1 gtttactaaa tgttttatta taaatttacc atatgaattt acgaaatttt actttgtttg
       61 ttttcaccat aaaagtaaac ttcgtttttt aaattatata gaatcaacaa acacaatttt
      121 actaatcaaa ggagtgtttt ttatgacaat tttagtacta ggcggagccg gttatatcgg
      181 ttcgcacgct gtagaccagc tagttcaaaa ggggtatcaa gtagctgtgg tagataattt
      241 gctcacaggt cacaaacaag cagtacatcc agatgctcat ttttatgaag gagacatccg
      301 ggataaagaa tttctacgtt ccgtatttga aaaggaaccg attgaaggag taatccattt
      361 tgctgcaagc tctttagtag gagaatctgt cgaaaaacca ttgatgtatt tcaacaacaa
      421 cgtatacggt atgcaaatct tacttgaagt catgcacgag ttcaacgtga ataaaatcgt
      481 attctcttct actgcagcaa cttatggaga gccgaaagag tctccaatca cagaagatac
      541 accagctaat cctaaaaatc cgtatggtga aagtaaattg atgatggaaa aaatgatgaa
      601 gtggtgtgac caagcatatg gcatgcgcta tgtcgctttg cgctacttca atgtagccgg
      661 agcaaaagca gatgcatcta tcggagaaga tcatacacca gaaacgcatc ttgtcccaat
      721 cattttacaa gtagctcttg gtcaaagaaa agccttggct gtttatggag acgattatga
      781 tacaccagat ggaacatgta tcagagatta tgttcaagta gaagatttga ttgctgcaca
      841 tattttagct ttggaatact tgaaagaagg aaatgaaagc aacttcttca atttaggcag
      901 cagcaaagga tactcagtca aggaaatgct agaagcagct agagaagtga caggaaaaga
      961 aattccagca gaaatagcac caagaagagc tggagatcct agccgattag tcgcttcaag
     1021 tgaaaaagcg agagaggttc tgggttggaa acctgaatac acagatatca aagcaattat
     1081 caaaacagca tgggattggc atgtcagcca tccaaatggt tatgaagaat aggggaatga
     1141 gatatgtcaa tcagtcaaac gatttgtgat tttgccacac ttgcgatcga agctggcgga
     1201 tggatggaaa tggaccgctt atatttacag aatcgtcttt tagggatggt aggagaagat
     1261 gctctagaaa gcattgaagt aagaccagtt aaggaagaat ctgtagactt attggatcag
     1321 cttgtcgcta aggcaagaga aaatggtgtt ctttcagaat tgccagcaga acaagagatt
     1381 tttgaagcgc aattaatgga ttttctcaca ccgccacctt cagtcgtcaa tgcattcttt
     1441 gcacagcact attcgaaaag cccagaggaa gcaactgagt atttctttaa tttaagtaag
     1501 aaaaatgact acatcaaaac aagagccatc gcaaaaaaca tcaaatttcc ggcagagtcg
     1561 gagtatggtg aactggaaat cacaatcaat ttatctaagc cagaaaaaga tccaaaacaa
     1621 atcgcagcag aacgcgaggc ggtaaaagta gactatccta cctgcatgct ttgcatggaa
     1681 aacgaaggat atagaggtcg cttgaactat ccagcccgca ccaatcaccg gattattcga
     1741 atgaatctag acggtgaaag ctggggattc caatattctc cttatgctta ttacaatgaa
     1801 cattcgatca ttttatcaga acaacaccgc ccgatgaaaa tcacaaagga aacatttgct
     1861 agactgcttc ggattactga agtcttgcct cattactttg ttggttcgaa tgctgacttg
     1921 ccgattgttg gaggatcgat tctttctcat gatcattatc aggcaggacg tcatgaattt
     1981 cctatggcaa aagcaccgat agaaaaactt atcaaactag cagattatcc gcaagtcatc
     2041 gcaggagtcg tgaaatggcc gatgtctgtg atccgtttgc aatcaacaga gaaaaaattg
     2101 ttgattgaag cagctaactc agtattggaa aagtggaaag catattcgga tgaaacagta
     2161 tctatcagag ctttttcagc agatggtaca ccacaccata cgattacgcc aattgcacga
     2221 agaagggaca attactttga gttggacctt gtccttcgag acaacaatgt ttcagaagaa
     2281 catcctgatg ggatttttca tccacataaa gatgtccaac atatcaaaaa ggagaacatt
     2341 gggttgatcg aagtaatggg gcttgctgtt ttaccacctc gtttgaaacc agaactagta
     2401 gaagtagagc atttcttatt aggagaagaa aaccagatgg ctgaatatca tcgtccgtgg
     2461 gcagaacaat taaaaaaaga acatcctgat ttgacgaaag atacggtcac cgatgtcgta
     2521 caaaaatctg taggtcaggt ttttgcacgt gtactggaag acgctggagt attcaagcga
     2581 gatgaaaaag gacaggcagc gtttgaacga tttactgcaa ttttataaac gtaaaaaagg
     2641 aggagagaca aatggcaaca attaaagaca ttgcgcagtt ggcaggagtt tctcctgcta
     2701 ctgtttcaag agttctcaac tatgatcctg atctttccgt tggcttagcc acaaagcaaa
     2761 aaatttttga agcagcagaa cgcttaaatt atacgaaaca tcgtaaaaat gcagcaaata
     2821 ctaagcaaac gcttcgcctg atccagtggt acgacgatac ggaagagctt gaagatcttt
     2881 attatttatc tattcgtcta ggaatcgaaa aaaaggcaga agaagcaaac gttactttat
     2941 tgaaagaaac gtgggattcc attagtgatc ttccgacaga tggaacgatc gcactaggaa
     3001 aattcgacga agctcagtta ttagaaatga aaggatccca aaatgccttg cttttggtag
     3061 attctgacgg cacttctcaa ggaatcgact ctctagttgt cgactttaag tcaagtgttc
     3121 aaaaagtcat tgaacatttt gtctcccata aagcgcaaca tatcgctatg cttgctggaa
     3181 cagaatatac gaagaaaaac catcagcggc taaaagatcc cagaattatg gcatttcaac
     3241 aagttttggc agaaaaaggc tacgacgaag gtccaattat cgaagcagat ttttctgtcg
     3301 agtcaggata tcaagcggtg aagcagtatt taaaaacgaa tccgcatgtt cctgatgcgc
     3361 tttttgcagc taatgatgca ttagcgattg gcgcactgaa agcgattcaa gaaaaaggtt
     3421 tagtggtacc agaagatatt tctgtgatgg gtttcaatga tgtcagtgta gcgaaatatg
     3481 taacgccctc tctttctact gtaaaagttt ataccgaatg gatgggagag ctagcagtgg
     3541 agacgattct tcaattaagt aattccaaag caccggtttc aagaaagatt attgtggaaa
     3601 cagagttgat tattcgagaa tcaacgaaat aaaagatgga aaagctgtgg ggaaatgaag
     3661 tgataccgca gcttttttgt gtttcaggaa ggggcaaaat t
//
LOCUS       DACDPS010000145         3633 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.147, whole genome shotgun sequence.
ACCESSION   DACDPS010000145 DACDPS010000000
VERSION     DACDPS010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.147"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            4..2376
                     /locus_tag="IWJ07_002661"
     CDS             4..2376
                     /locus_tag="IWJ07_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334179.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERIENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAESGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVTKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKTGQTELQVGIQAVAKNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFSLGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2437..>3633
                     /locus_tag="IWJ07_002662"
     CDS             2437..>3633
                     /locus_tag="IWJ07_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAP9334180.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAK"
BASE COUNT         1420 a          582 c          787 g          844 t
ORIGIN      
        1 aacatggcag aagaaaaaga accactagac aatagtcgtt tgggaaaatc caaaagaaaa
       61 ctagtacgat tgcaaaatga attaaacgaa caaattgaaa agatgtttga acaccaaaga
      121 aagaccaacg gtcaaccaat gaacgataag agaaatgggc attcttggtt taggcaacaa
      181 gagcgaatcg aaaataaagt gcatagtctg agagaagaga tcaagcaaca agaaaaacag
      241 gtagaaaaac ttgaaagaca agaagagcta aaggaaatgg gctataacaa gtacggcgga
      301 ctggatatga cgatcgataa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcga ctttttcagc cgccacgatc cgaaaatacc aacggaagtt ggaaacatta
      421 gaacagctga aagaaaaatc agaaaaagga aaagaaaata tcctccctga agtacaagcg
      481 atcattgaca gcggacgagt gacgcaatgg aaaaaaaatc caaccattta ttttttgaaa
      541 ggctatcgaa aagttgcttt agaactgaat gaaaacgggg gatttgagga atcaaaaaaa
      601 taccaagcca cgtcggaaga ggaaaaagca gctgttcgtg aattacttga agaggctgca
      661 ccaactgatt ctctgaagga gaaagaaaaa gatcatttct atcgagtaga attcaacgaa
      721 aatgatccag aaaaaggatt gaaaagttat ggtggggaga tcgtaacatt agagttgatc
      781 gatcaactta aaacgttgga taaccaatac gctgcagaat caggttatta taaattctat
      841 tttgaagaag tgaagaacgg agaagtagtc gatcagatcc gaatggatct aggggatggc
      901 gtcaacagta atcaacccat ctatttagag ttagcaaaat tgtgcgaaca agtacctgag
      961 aaggaacaaa aacaatcggt caaaaatgaa cctgttacga aagaacaaat gcagtcgaaa
     1021 gaaacaaaaa caaaacaaaa gggctatcaa aaacgaacgg cagaagagat taaacaagaa
     1081 gttaaatccc tttcgcaaca ggcactggat gcggtgaaaa agcataccca ttctcctgag
     1141 gacgtcaaag aacttttgaa ctttatgtct cgttttccag aacgatcctt tagaaatcaa
     1201 ttattgattg aggaacaatt tccaggtgca acggcatgtt taggtcgtgc cgcgttgaaa
     1261 aaagaaggaa tctatatcaa aaaaggggaa caagggaaca agatctttgt cagaaaaacg
     1321 gtcaaaggat tttatgagaa aggacgtggc tttgttcgag aaacagaagc tacaccagaa
     1381 gataaaaaac gaatcgaaag tggtcaaatt gaagtgatca aaaaacctta ctacacgatt
     1441 gaaaaagtgt ttgatatcac acaaacacaa ttgaagcctg aagactatcc gaaaatcttc
     1501 ccgaaccgcg tctttgattt tcaattggat aaaacagggc agacagaact tcaagttggg
     1561 attcaagcgg tagccaaaaa tattggaatt caaattcgag atatgacaga aagtgaagtc
     1621 tatcaaagag aattaggaca agcacgtggg gcgtacgttc gtaacgaatt tactggcgaa
     1681 gaagaaatcg tcatgaatac tcgaaatact cctacgcagc atttagctac aagtattcat
     1741 gaattagccc atgcaagaat gcacaaattt tcagaattag ataccgcaac taaagaattg
     1801 caggcagaaa tgacttctta tattgtgtgc aaacattttg gaatggacac gtcggagaaa
     1861 gctattcctt atattgcagc atggacgaag aatggtcaga cgttggatga taaggaagct
     1921 gcggaacgtg ggaaaatcat gaatgatgtc agccgtgtcg caaatgaatt cattcaaacc
     1981 atttctaatg aaatcaatca atacagagaa agagaaccag agatccaacc agcagaagtg
     2041 aagcaggagc ataaaaatac tgtagaaacg gcagaacaaa agaatattgt tcctgttggt
     2101 tcactttgga tcgacaagaa agacgggaaa gtaatggaag aagatacggg tcgaacgtta
     2161 catttgaagg atgcggcgtt tattgaagat ccgcagcata atcaagtgat tttaaaagga
     2221 aaaagttatg gaaaagagat tcaaacggcg ttaccttcag aagaattttc gctaggaaat
     2281 ccattccgat tttcacttga aacagtgtca gaagtggcca aacgaaaaaa tgaaatctat
     2341 tatgaacaac aattgtcaca aaaagaacgc gtataaaaca agtaaaaaag actatctgaa
     2401 aaacggatag tcttttttct tttgggaggt ttgaaaatga ccgaaaaaaa aatgagtctt
     2461 attgatcgat gtaaacagat cgatatagtg gattttgctc gaaacaatgg aatggctgta
     2521 gtcaataaag gacgagacta tcgcttagaa gaccatgatt catttgtttt tgatcgaaga
     2581 aaacaacgtt tttattggaa cagtcaaaat attagtggcg acattattga attagctaaa
     2641 ctttttttca tagacaaaga gatccaagac tctaagcaac aatttaaagc agccacagat
     2701 tttattctaa agaatgaaga caaaacagaa cgagtagaaa atcttcattt tgagacagaa
     2761 aagtacaaag atcaccccgt tgattatcaa ccactcacag aaaaaggaag gaattatctg
     2821 aaggaagaac gaaaactacc agagtggctg attgattatg cagaaaaaga agggttgatt
     2881 gccgaactaa agccaaaaca tgaacggcaa aatttcttag ttcgagacga tcgtttagat
     2941 catgcggtcg cctttttatg gaaagatcca cagacaaatg agacagtagg ggcgagttat
     3001 caaggaacgt tcattgatta tgaacgtttt ggtgagagag gcacatataa gcatatagat
     3061 aaaaattcga cagcgaatca tggctttaac ctaaagatcg gtgaccctaa gcagttaaaa
     3121 ttttttgaga gtagtattga tttacttagc tatgctgcac tgaatcgtga tcagttaaac
     3181 gatacgtggt tagtttcgat ggaagggtta aaacaccatg tgatcagtca ttattttggt
     3241 gaggcagtat ctgaattgag aaagaaacaa gcctttcctc aatccatcga gatttgtgtg
     3301 gataatgacc gtgcaggaca tattttctac gaaaaagaac aattaatggg cgcagttgat
     3361 ccatttacga atcagaaagt acgttgtgaa cgaggaatcg ctaatgattg gcaagtgcca
     3421 aaagaataca aagtcattta tgaagaagtc gcaaaagaag aaaagatcac accagaagct
     3481 attatggcca tacacaagac agaaaacaat ctgcaattaa cgaaccaatt ggtttccgct
     3541 cacaaagtaa atgcgtcttt tggtcagcag ctttctgtta acgattcaat agaagctatt
     3601 aatttgaaag atatttgtag agaagtcgca aaa
//
LOCUS       DACDPS010000146         3575 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.148, whole genome shotgun sequence.
ACCESSION   DACDPS010000146 DACDPS010000000
VERSION     DACDPS010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.148"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..407
                     /locus_tag="IWJ07_002663"
     CDS             <1..407
                     /locus_tag="IWJ07_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334181.1"
                     /translation="GGYFLLNLDSVNSNAQIKDQRVAEHNNKKMSLTKNIYSMSDEEL
                     YKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSELVSYKQNHRQFIDVNTD
                     SNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            421..1110
                     /locus_tag="IWJ07_002664"
     CDS             421..1110
                     /locus_tag="IWJ07_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334182.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1235..>3575
                     /locus_tag="IWJ07_002665"
     CDS             1235..>3575
                     /locus_tag="IWJ07_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAP9334183.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRS"
BASE COUNT         1207 a          468 c          755 g         1145 t
ORIGIN      
        1 ttgggggcta ttttcttttg aatttagatt ctgttaattc aaacgctcaa ataaaagatc
       61 aaagagtagc tgaacataat aataaaaaaa tgagtttgac caaaaatatc tattctatgt
      121 cagatgaaga attgtataaa gaatttggtg atttatccgg ggaacagtta gaagaaagta
      181 ttccaggaat tagtgactca gataaaattg ttgtcttgtc tgctggtggg caattaatgg
      241 gagatggcaa atccgaatta gttagttata aacaaaatca cagacagttt attgatgtta
      301 atactgattc caacgctatt tcgattaagg atctaaaaga gcttttaaat agtaaaagaa
      361 atgaattaaa gcagataaaa gaaactaatt cagtggaatt agagtaaagg aagattcatt
      421 atgaaaaaga tttttggtgt ttctgttggg ctacttatga tgttaacatt tggaaatgca
      481 gtatatgctg ctgagaaaga agttatttca gatccagagc agtacgcctc acgatacttt
      541 gctgattttg aagaagaaga taaagtactt gcattgccag aaggtggttt tttaaaaggg
      601 gaagctaaaa ttgtatctat ggataatcca gatataattc aaggttcata taattcagaa
      661 actgatcctg cttcagtttc agtgacagaa gcaaaaaaaa ttgttgctga aaaattcaaa
      721 aatgatattg aaactactcc aattatgcct cgaggagcat ttccacccga tgttggtact
      781 agcggacacc ttcttgtact agcaggagga ggaagctata aatcacagcc atttagtggt
      841 gcgggatggc gttttggagg tatgggtgta aaaccagctg atggtacagg caacttttta
      901 cgttggaaat cctacattga cgatggaaga gtaggaaatg taaatcaagc caacgcaact
      961 aaatctggtt ctattcaagg aatggcattg tatgctcgtg aaggattcaa atactttgca
     1021 ccaaacgggc ctggttggac aatgaatata ggtatttatt acacttataa tcctgttgca
     1081 ggtacgtatt atgttgttca aaatatttaa taaaacagca caaagagatt tctttgtgct
     1141 gttttattat ttttagttgt tgacaaacac taaaatataa aatataatca acacataaac
     1201 gaacgtatgt ttggcaagaa agcaggggat ttttatggat gagatggttt taagtactca
     1261 gaaatggcta aacaagaaat acagtaatgt tacagggttt gataaggttc cagaaaatgg
     1321 gcgaacaggg tggcctacaa tctatggact tattgaagga ctacaagtag aacttggtat
     1381 tactaatcta gtagctaatt ttggacctac cactgaaaaa atgtacgata atcaggttac
     1441 tcctaaatgg ggcaaaaatc tacctaaaaa tattgtattt cttattcaag gtgccttttg
     1501 gtgtaagggg attaatccag gtggcttcga tggggtatat actccatatt tagatactgc
     1561 tgtaaaagag ttgcagactg atgcagggtt taatggaagc gctgtaactg gagttttgga
     1621 tgcgaaatgg gccaaagcgt tatttgatat gagtgctttt gttcttgttc ccggcggaga
     1681 tagtaaaatt agaaaaatgc agcaatggtt aaatgtaaat tatttagaat acacgggaat
     1741 aatgccttgt gatggtattt atcaaagaaa ttcaaatcaa gcattaattt ttgcattaca
     1801 agctgaacta ggatattctc ccagtgaagc aacagggtct tatggtaatg ggacaactag
     1861 taaaacttat cctgtgtctg aaggtaactc gggaaactat gtaagaataa ttcaatatgg
     1921 attatatgtt aatggttatt ttgaagacgg tacattcgat ggtattttca ctaaatatat
     1981 gggtttagaa gtactggcat tccgaaaatt tatggtattg cctgaatata ctgaaatagc
     2041 agatgttgtt gtaataaaag gactattgtc tagcgctgga gatattagaa gatctgcaga
     2101 tggcgcagat acttctacgc aattaactag atctcaaatc caaacattgg ttgataatga
     2161 tataaaaata gttggtagat atcttactgg tactgttgga attggaaaag atgaacgaaa
     2221 taagtatcta actacagagg aattgaataa tattttttct aaaaatttat ctgtgtttcc
     2281 tatctatcaa gatggaggtg cagcattagc ttattttact tatgatcgtg gcttatcaga
     2341 tggtaagaag gctatagatg cagcaaaaaa tctaaatata ccattaacaa cagttattta
     2401 ttttgctgtt gatctagaca tgttagggga agaaattctt gcatatgctg gagaatattt
     2461 taagggggta tctgctgtaa tgtcatatag cgggtatcaa acgggtgttt acggaacaag
     2521 aaatgtttgt tctcaaataa tcaataacgg gtatgcttcg tttgcatttg tttcagatat
     2581 gtcgactggt tatagtggga atttaggttt tcctatgcct agacaatggt cttttgatca
     2641 atttgttgaa tttaccatag gaagtggcaa cggagctgtt ggtatcgatt taatcgctac
     2701 atcgggtaga gatagcggat ttaatgagtt atcaaatgat actaataatg ataattacat
     2761 tgccaaatac aatcagaagg ttataaatca gatggtacgt gcgtatcaat atctatcaca
     2821 agctggtctt gataatcctt ggaaccctca tttgacattt tatagatatg taaattattc
     2881 tggtttgtca tgggacatca tttcttctcc tgtaacagag catgatagaa aaatttatga
     2941 tgaatatagg aataaattaa ctaatagcga gggcctgtat aattatttta tagatcctaa
     3001 tactggttca ataataggtt tgcctcactt aattgtgacg ttacaatcac agatgtttct
     3061 agccgacaca atcaatgata gtgtttctga ctttgcaggt tggttaggag atttaatgac
     3121 ttgctggggt gaagttaaga aattaggaat acaaatggag caaggtgtat ttgcattagt
     3181 aggtacggct gcaggtggaa catttagtct agaagattta taccaagatg ttgacgcggt
     3241 aaatgtttat accttagcaa aaagaaattc tgattcaaca gctttatcgg ctttaaaaac
     3301 gtactattgt gataagggtg ctattgatag atttaatcaa tttgttaaga acagatttgg
     3361 ttcttcagat aaaatcgctt cagtaacaaa tagtttatta gaaaatgacg aaacagttga
     3421 tatcttaggt gtagggataa tttcattttt cattgataaa atagttaata attcacttgg
     3481 gacagatttt acttatcttg attttattgg agatgagggg aaagttgcaa atggattttc
     3541 tgaaaaaatt attcaatttc aagaaaatag aagta
//
LOCUS       DACDPS010000147         3572 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.149, whole genome shotgun sequence.
ACCESSION   DACDPS010000147 DACDPS010000000
VERSION     DACDPS010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.149"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            49..471
                     /locus_tag="IWJ07_002666"
     CDS             49..471
                     /locus_tag="IWJ07_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334184.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRDGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            481..684
                     /locus_tag="IWJ07_002667"
     CDS             481..684
                     /locus_tag="IWJ07_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334185.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            895..1479
                     /locus_tag="IWJ07_002668"
     CDS             895..1479
                     /locus_tag="IWJ07_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9334186.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            1850..3175
                     /locus_tag="IWJ07_002669"
     CDS             1850..3175
                     /locus_tag="IWJ07_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP9334187.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRS
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLNIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQINEEN
                     LDKIIDKIRQKYGFTSIVHASSMLESARSITRSTLVGGHAGGNGGIKND"
     gene            3168..3518
                     /locus_tag="IWJ07_002670"
     CDS             3168..3518
                     /locus_tag="IWJ07_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334188.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIVGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
BASE COUNT         1273 a          528 c          704 g         1067 t
ORIGIN      
        1 atttattaag aaaacgtttt cttagttttg atttcgaggt gatagacaat gaaaaataag
       61 tttattttat ctttaggtat tttaactatt ggaattagtg taactggaac tacaattcct
      121 acatttgctg atagtacaaa tgaaccaact actgtagtag ataataacca agaattaact
      181 cctttagata gggacggaat agccattggt aacccaacta acgacttaac tgttactgaa
      241 aattatttaa tagtttctcc attacttgct cgagcagctt ctaaaacgat acgagtgaca
      301 ggaacgatga tttttgataa agttggttgg ccttctagac ctcctcaatc acggaattgg
      361 aaagaaaaaa gaaatggtta ttggtactat ggaaaattgt atttacaaag ttataaatca
      421 gctggaaaca attggattgc gacttattca ggaaccttaa ctagaaagta gggatttttt
      481 atgccagaat ttgtgaacac accaaatttt tatcaagata gcgatattct aagtaaaatt
      541 aatttaccga aagaagaatc agctttacaa gttcagcgaa ctttacagga gacacaaaaa
      601 attcaaaaat caatttctaa tctagaagat tctgaagaaa aaagaaaaaa aagagaccaa
      661 aaaacatatc aaaaagaaaa ataatgattt taaaaaaaga gtgtctagtc gacattcttt
      721 ttttctaatt gaaagtgttc ataaaatcac ccatttctgg acacttcctt tttttgcttt
      781 atttttcaaa aaaacaattc atttcatgtt cataaaccca taacaatatc tatgttcagt
      841 taaactatat ggataaagtt atgttacaat aaaattaatg aaggagtgat ttttttgaaa
      901 ataggatacg cccgggtatc tactggttta caaaatttgg atttacaaga agacagccta
      961 aataaatttg gttgtgaaaa aatttttact gatcatatga gtggggcaaa aagtaatcga
     1021 ccaggattag aaatggccat tgattttgtt cgctctggcg atacacttgt cgtttggcgc
     1081 ttagatcgtt taggtagaaa tatggaagat ttgatttcaa tcgtcaatcg actgaatgag
     1141 cgtggtgtga gttttcatag tattcaagaa aatattacga tggacaaatc aagttctaca
     1201 ggacagctca tgtttcattt gtttgcggca tttgcagaat ttgagcgtaa tttgatcctg
     1261 gaacgatccg cagcaggacg agcagctgct cgtgcaagag gacggtttgg tggtcgtcct
     1321 gaaaaattgt ctgaacaaga tttggaatta ctgaagactt tagtggatag cggtacacct
     1381 attaagacta ttgcggaacg gtggaacgtc tcaagaacca ccatttatcg ttatttggca
     1441 aaaatggagg agaaaaatgg aaagacaaat tctcattagt ttttgtcatt taatgctgaa
     1501 aatttttgcc atttatctag aaaagtgaca atagtatgag aattttttgt aagtgtctac
     1561 tgataactta ttatcaaatg ttgtaaggat tttattcatt tgttaatatt aaagaacgga
     1621 actccaaaaa agtccgtact ctaatttgca gtctctcact cttttagagt gagagttttt
     1681 attagatttt caaattaatt agtaaagtga tagaataaat tgatcataca aatcataaga
     1741 agaagcaggg tgatagcata cctatgcttc tttttttatg tatttagcta tttcttcttt
     1801 acaacatcga acatatgttt gtataatgtg gaagaatgga gtgttattta tgatggatta
     1861 ttcaaaagag ccagtaaatg attacttttt aatcgatatg aaaagctttt acgcaagtgt
     1921 cgaatgtata gaaagaaatt tagatccatt aacaacagaa cttgttgtta tgagtcgatc
     1981 tgataatact ggttcaggat tgatattagc ttcttctcct gaagcaaaaa agcggtatgg
     2041 tattacaaat gtgagtagac cacgtgactt accacaacca tttcctaaaa cactacacgt
     2101 tgttccacca cgtatgaacc tatatatcaa gcgaaatatg caggtaaata atattttcag
     2161 aagatatgtg gctgatgaag atctactgat ttactcgatc gatgaatcga ttttaaaagt
     2221 gacaaaatca ctgaatcttt ttacgactga agaaacacga agtcagcgaa ggaagaagct
     2281 cgctcaaatg atccaagaac gtattaaaga agagttaggt ttgattgcta cggtaggcgt
     2341 cggcgataat ccccttttgg ctaaattagc cttagacaat gaagcaaagc ataatgaagg
     2401 atttattgcg gaatggacgt acgaaaacgt tcctgagaaa gtatggaata ttcccgaaat
     2461 gacggacttt tggggaattg gttctcgaat gaaaaaaagg ctcaaccaaa tgggaatttt
     2521 gagcattaga gaccttgcta actggaatcc ttatacgatt aagaatagat taggtgtgat
     2581 tggattacag ctgtacttcc atgctaacgg aatcgatcga acagatatag caatcccgcc
     2641 agaaccgaca aaggaaaaat cttatggtaa ttcacaagtg ttaccacgag attatactag
     2701 aagaaatgaa atcgagctgg tggtaaaaga aatggcggaa caagtagcca ttcgtatcag
     2761 gcagcataat tgtaaaacag gatgtgttca tttaaacatt ggtacgtcga tcttagaaac
     2821 gagaccagga ttttcacacc agatgaaaat acctatcaca gataatacaa aggaattgca
     2881 gaattattgt cttttcttgt ttgataagta ttatgaagga caagaagtaa ggcacgttgg
     2941 aataacctat agcaagcttg tgtacaccga ttcgcttcag ctggatttat ttagtgatcc
     3001 acaaaagcaa attaacgaag aaaatttaga taaaattatt gacaagattc ggcaaaaata
     3061 tggttttacg tcgattgtac atgcttcttc aatgttagaa agcgctagaa gcattactag
     3121 aagcacactt gttggtggtc atgcgggcgg aaatggggga attaaaaatg attgattaca
     3181 atgatcgtgg acgtttaaag tgggcagggt tttatttatc tgatcatact gaagaaattg
     3241 atgccgacaa tgaacaacga accagtcaaa atttagcaaa agaacaaatg acgactgaag
     3301 agattgcgga agtattgaac gctgcagtca taaaaaatag atcagtatct attcaaaaag
     3361 aagaacgaaa cgctgaagga tactatccag aagatattgt tggcaagatc ttaggtagtg
     3421 atgagctggg gatctacgtt ggaaaagaca aaattcacta cgacgaaata cgtcatatcg
     3481 aattttacca ggataataaa tggagtcgat tagaatgaac attatccagc aatatgagct
     3541 aaagtatata acctttgatc agctatcaga ag
//
LOCUS       DACDPS010000148         3477 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.151, whole genome shotgun sequence.
ACCESSION   DACDPS010000148 DACDPS010000000
VERSION     DACDPS010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.151"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..228
                     /locus_tag="IWJ07_002671"
     CDS             <1..228
                     /locus_tag="IWJ07_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9334189.1"
                     /translation="VKHGIPMEELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMN
                     EQYGVKNIYFQATPSAPKTGLKDVLNKFDLS"
     gene            265..666
                     /locus_tag="IWJ07_002672"
     CDS             265..666
                     /locus_tag="IWJ07_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334190.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            778..1131
                     /locus_tag="IWJ07_002673"
     CDS             778..1131
                     /locus_tag="IWJ07_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334191.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            1165..1608
                     /locus_tag="IWJ07_002674"
     CDS             1165..1608
                     /locus_tag="IWJ07_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP9334192.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            1625..2710
                     /gene="uxuA"
                     /locus_tag="IWJ07_002675"
     CDS             1625..2710
                     /gene="uxuA"
                     /locus_tag="IWJ07_002675"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP9334193.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            2703..3416
                     /locus_tag="IWJ07_002676"
     CDS             2703..3416
                     /locus_tag="IWJ07_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9334194.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
BASE COUNT         1222 a          526 c          785 g          944 t
ORIGIN      
        1 gtaaaacacg gaatccccat ggaagaattg aatattggag ggattgctca aaaaaatcct
       61 gatacagatc ttttagtgac gaaatctgtt tatttaaata aatcagatgc tgaaaaattg
      121 aaagaaatga atgaacaata tggcgtaaag aatatttatt ttcaagccac tccatctgca
      181 cctaaaacag gattaaaaga tgtattgaac aagtttgatt tgtcataaac aaatgcttag
      241 aaaaaagata aagagggcga aaaaatgaag aagaacgagc tgtttcgtga ttgggaattt
      301 cgttatcgct atatttaccg taagcgccgt acaaaaaaga gtaaacagcg ttttctttca
      361 gcattagtat cagatattta cagtatgaga acagatgtga cagtcattgc ttacgatacg
      421 ctagcctacc gatcaaaaaa tatttatgtt ggagatatcg aaaaagcaga gaaagttatc
      481 tgtacttatt acgatacacc cgttcatgca ttaggaagtt atttcatgtt tgattggaaa
      541 gaccaaagga aaaaaacaat ctactctata ttattgtcct tcattttgtt gttcagttta
      601 ggttggtggg gaatgatgat atataacaaa aatcctcatc acgtattcga tctgctatcc
      661 gtataaacag gtatcactgt actagcattt gggagctact ttttcttatt gggaaaagca
      721 gctcgcggat ggcctagcag acaaactttt attcgaaata cttcatccat attaacgatg
      781 ctagaaatga tccgtacgat tgatgatccg agtgtagcgt atgcgtttgt tgatgaaggc
      841 tgttatggag aaaagggctt agacagcgtg agatcgggta tgaaaaaaga ggctattctt
      901 ttttatctag actctgttgg ggcagataca ccgctacagt tttcgggaaa ttatttttca
      961 aataaagaac agtggctgaa acaagttgat aagttgaaag aaaaaaacgt caattatatt
     1021 tttagtgcaa gaaaaaagca agcgcagttc ttctatttga ccaaaacaga tttacgaggg
     1081 aaaacattta attggcaaaa tgccaatcag attattgctc tttttcgata agtaaaaagt
     1141 gcaaaacaag gagtgagaag actaatgttg gaaatcatga tcgttacaca tggtaagttg
     1201 agcgaaggac tgaaagatgc ggcggaagta attgtcggga catcgagcca tattgaaaca
     1261 atgtgcttag agtctgggga ggacatccaa acattcggag agaaggtcaa agaaaaatta
     1321 actatgatca acacagcaga acccatgcta gtatttgttg atctaattag cgcaagtccg
     1381 tacaatcaag cgctcttggc agtcaatgaa ctgactgaag aaaagaaagc agccacttat
     1441 gtcatagggg gcgtgaatct gccaatgttg ttagaagcaa tcaatcatca gatgcttcac
     1501 acaccgatag aagaagcggt ggaacaaatt gtcaaacaag gaaaagataa tttaactgtt
     1561 tggcatgctt cagcggaaga aaaaaaatta gatgaggacg atttttagaa ggaaggctga
     1621 aaagatgaag tggggattta gatggtatgg tgaaaaaggg gattcgatcc cattaaaata
     1681 tgttcgccaa ataccgggaa taaatgggat aattggaact ttgctgacta aaatgcctgg
     1741 agatgtttgg gagatttcag aaattgagga gttgaaagca tctgttgaga gccaaggact
     1801 ggaactgctt ggaattgaaa gtgtagcgat ccacgatgca atcaaggcag ggacagatga
     1861 acgagattac tatattgata actatatacg aacaatcaaa aacttgtctc gatgtggggt
     1921 tcgattgatt tgttatagct tcaaaccaat ttttggatgg gcgaaaactg atttgtttaa
     1981 tgaaaacaac gatggaagct attctctttt atacgatcag aaaatcgtcg atcagatgga
     2041 gccaggagaa atgtatcaat tgatacacag ccagtcaaag ggattcaaat tgccgggttg
     2101 ggaagaagaa cggttgaaaa aattcgctca gctggaaaaa ctgtatgaag gaatgaccga
     2161 agaagaatta tttgaaaatc tgacttattt tttagaaaaa gtgattccag tgtgcgaaaa
     2221 agcagatgta aaaatggcta tccatccaga tgatccgcca tgggaacttt ttggttttcc
     2281 tagagtgacg aaaaatcttg gcgacttgaa gaaaattcta aatgcagtag attctccata
     2341 taatggacta acgctgtgca caggatcttt aggggcaaat ccggaaaacg atttagttga
     2401 aatcatccat gaggtaggtt cgcgcatcca ttttgtccac ttcagaaacg tggagttttt
     2461 aggagaaaga aagttcaaag aaacggcaca tccctctaca gaaggctccc ttgatatgta
     2521 tcatattatg aaagcgttgg tgcaagaagg gttcgacggt cctatacgcc ctgatcatgg
     2581 tagaaccgtg tggggtgaag tggctatgcc tggatatggc ctgtatgatc gtgcaatggg
     2641 actcacttac atgcaaggtc tatatgaggc gataagtaag gaaaaaccac tgggagagca
     2701 ttatgaataa aatcaagctc ttcatctttg atatggatgg ccttttatta gaaactggac
     2761 gtttagctta tcgtgcttat gtaaaatcag cacaaaagta tgattatgaa atgcgtaagg
     2821 aagtctatta tttactaact ggccaaacag aaatggcgat aagagaacaa atgggcattt
     2881 tgtacggaga agatgttcca tatatcaaat ggcgagaagc aatcaatcaa tataaagaaa
     2941 aaatcgtgaa agaagataag agagtatata cgaaaaaagg agcagaagaa attttatctt
     3001 ttgcaaaaga aagaggaatc catacaatcg tggcttcgtc taatacacgg gtaaaagtag
     3061 aaatgtattt aaaaatggaa aatctatatg ccttgtttga tcaaatcatt tcaggggatg
     3121 atgtaaaaaa aggaaagccg gaaccagaga tctttttgaa agcttgttca aaaatgaata
     3181 ttcctccaag tgaagcactt gtatttgaag actcgattgc agggatcgaa gcagcaaggc
     3241 gagcgggcat tttgtctttt ctcatcaaag atcatttgat agggttgccc gatcataatg
     3301 ggaagcataa gttgaaagta gatgtaagtt tatatgaatc caataaagca gactatgaat
     3361 tttatgacct gcatcaagca aaacgatttt tagaaagtaa agggttgtat ctctaataaa
     3421 gaggactatt ttctagtcct ctttatttat tgtcctgcac gactgaggga acagatg
//
LOCUS       DACDPS010000149         3472 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.152, whole genome shotgun sequence.
ACCESSION   DACDPS010000149 DACDPS010000000
VERSION     DACDPS010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.152"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(516..791)
                     /locus_tag="IWJ07_002677"
     CDS             complement(516..791)
                     /locus_tag="IWJ07_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAP9334195.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            899..1663
                     /locus_tag="IWJ07_002678"
     CDS             899..1663
                     /locus_tag="IWJ07_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAP9334196.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLADVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            1786..2289
                     /locus_tag="IWJ07_002679"
     CDS             1786..2289
                     /locus_tag="IWJ07_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9334197.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            2697..3344
                     /locus_tag="IWJ07_002680"
     CDS             2697..3344
                     /locus_tag="IWJ07_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HAP9334198.1"
                     /translation="MEQTIKPYQYFFIIREVEQLLNAYSSVNDPKTVQTVQALAAEKI
                     RDILDHELPEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
BASE COUNT         1159 a          623 c          701 g          989 t
ORIGIN      
        1 ctaaaaactt tttttattct gcccgaaata gaggagtcaa atgaactcac tgcaagtgat
       61 agatcagctt ttttactacc tgtctttaat acttggggca aagcgcctca aaaacaacct
      121 tatcgacggt gttcaaaagt cagtttcttg gtattaagac aaatttatgc aaaaatgtgg
      181 aaagccattt tcacaaaatt ttccttaata ctcaaaacta agcgactttt tcacaaccag
      241 tccttaatgc ttgatgcaga ccagcttttt cacaacctta ttgacggtgt tttatcagct
      301 gcctctcggt attaagtcat tacatttcaa aatatgagaa gctattttga aaatgtttct
      361 ccttaatact cgaggcaaag cgcttcaaaa acaaccttat cgacggtgtt caaaagtcag
      421 tttctcggta ttaagtcaaa tttatgcaaa aatgtggaaa gccattttca caaaattttc
      481 cttaatactc aaaactaagc gaccttttca caaccttatc ccaccacatc aaatttcttt
      541 cgttcctcaa tcatcggatc ttcttggaca tccacatact gcactcgtcc acttgggctc
      601 ggagacttct tgacttcttc gataaatttt tggatgatgt cgtcatttcc gattgcttcg
      661 attgtgactg agccgtcgtc ttcgtttttt accgttccgg tcacgcctaa ctgatcagca
      721 accatcttgg tcatatagcg aaatcccacg ccctggacac gtccttggac attcatttta
      781 actttgcgca tgttcgtcac ctttctttcc tgttttataa atcaatgata ccgatttttt
      841 taggatgaaa caagctcaag cacctattcc atgatataat caggtgaggt gaaggaatgt
      901 gaaagagatc caatcagcaa aaaatccaag aatcaaagaa ttaaaaaaac tacataaaaa
      961 gaaatacaga gaagaaaagg cagaatatct tttggaaggg ttccatctga tcgaagaagc
     1021 tgcgtcgtct gatgcagaaa taaaagaact ttttgtgaca cagagaggta tgaaagaatg
     1081 gggagactgg ctggctgtaa acaaagagat ttctactttt ctagtttcag atgaggtcat
     1141 gaccgctatg tcagatctac ctacaccgca agggatctta gcagtgatga cgttaacatc
     1201 tgaagatacg ccagattttt caggaggctg gcttttacta gataatgtac aggatcctgg
     1261 aaatgtggga acgatgatcc gtacggcaga tgcgtttggt atggctggtg ttttattagg
     1321 taaaggaacg gcagatattt atagtacaaa ggtgctgcga agcatgcagg gaagcaatta
     1381 tcatttgcct gtgttacaaa aagatttggc agatgtaatc cctgtaatga aaggcaaagg
     1441 gtttacggtg tacggtacag aattaaatga agaagcacat gcattagaca aagtagaaaa
     1501 aacagaggat ttcgctataa tcatgggaaa cgaaggtcag ggagtcagtc aggaaatctt
     1561 gtctttgact gatcagaacg tatatatccc aatgaaagga aatgcggaat cattgaatgt
     1621 cggagtcgca gcagggatcg ttctttatca tttttcttta tgattaagga actattaagc
     1681 tttagctatt ttatctagag cgactagaat aatccgaaca ctggtggtat acttagatca
     1741 atttcagaag aaaatgaaat tcaaaaaggt ggcagatcga taattatgac agaaaaatgg
     1801 cgtgaagacg aagaatatat gtcttatgta caagatcttt tagaaacaga agcagtgaaa
     1861 aaattagcga attacacaca gcatgttcat tccactcgtt tggagcattc catcagtgtt
     1921 tcttactata gttatctgct cgcaaaaaaa tggggtggca atgctaaagc aacagcgcgt
     1981 gctggacttt tgcatgatct gttctactat gattggcgta cgacgaaatt tgacgagggg
     2041 acacatgcgt atatccatcc aagaattgct gtaaagaatg cagaaaaaat caccgatttg
     2101 tcggatcttg agcgagatat cattttgaag cacatgtggg gtgcaacgat cgcaccgcct
     2161 aagtataaag aaggatatat tgtgacattc gtggacaaat actgcgcagt caaagaagcg
     2221 gctcagccca tgtctgcaag tatgcgtaag agatggcaac gttactttgg gaaagaatcc
     2281 tcgatttaac aggtaaaacc aataatagac catattgagg ttgtgacaga agtggtcagc
     2341 ttcaagaaat aagaaggaat ttacgaaaat tacttctcaa atttttgtga agtttcggct
     2401 tattactgca gatgcaagct tttgaacacc attcattcga aacaagggac tttgacacga
     2461 cttttgtcac agtccttttt tctgtttgca gttgactatc tgttcaagat acggtttact
     2521 tttatttatc gaatgaatca tgtacataga ataataagca ttaaaggggg tgatgataag
     2581 atgcaaaacg tatcttattt aaaagagtcc aggatcttca ggtaacacat actttgcaga
     2641 agatcacttt tttctacaaa gtatgcactt tggttagaag aaaggacttt aaaatcatgg
     2701 aacaaacaat aaaaccatac caatattttt ttataataag agaagtagaa cagttattaa
     2761 atgcgtactc atctgtcaat gatccaaaaa cggtacaaac agttcaggca ctagcagctg
     2821 aaaagattcg agacatatta gatcacgaac tgccagaaat cgatacattt ttaacgacag
     2881 ttttagacgt aaaactgaca aaagcgcaag cagaaagagc attggaggag ctgaagcaaa
     2941 tcgttcagcc gtttatgcag cctagcaaac cacaaatcga gaaattattc cgaaaagtga
     3001 aaaaactcaa acaaccagct tggaatgaaa tcgacttgca agaacatacg tacatcggct
     3061 ggaatgatcc aggtacgcag aaaaaattca tggtctatta cgaagatgaa aaattaaaag
     3121 ggattagcgg tactttatca ccaacgataa ataagggaat ctgtgcaatc tgtcagaaaa
     3181 catcgaatgt ttcgcttttt ctttctacaa cgaaagcagg ttctgatggc acgtatacga
     3241 aaaaggggaa ttatatttgt catgatagtg accaatgtaa tcaacagtta acgaaattgg
     3301 ctcctttcta tgaatttgct gaacgtgtaa agaaagaacg ataattttat tgttttttac
     3361 agaaaaatat gctatctttt aatggaagtg aataggtgat ggtgttcgcc tttaaatgct
     3421 aacgaaagat agctaatgac gcctactaaa gtctggggac agcccctttc tt
//
LOCUS       DACDPS010000150         3401 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.153, whole genome shotgun sequence.
ACCESSION   DACDPS010000150 DACDPS010000000
VERSION     DACDPS010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.153"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(542..1558)
                     /gene="tsaD"
                     /locus_tag="IWJ07_002681"
     CDS             complement(542..1558)
                     /gene="tsaD"
                     /locus_tag="IWJ07_002681"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAP9334199.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(1555..2019)
                     /gene="rimI"
                     /locus_tag="IWJ07_002682"
     CDS             complement(1555..2019)
                     /gene="rimI"
                     /locus_tag="IWJ07_002682"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP9334200.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2026..2568)
                     /gene="rimI"
                     /locus_tag="IWJ07_002683"
     CDS             complement(2026..2568)
                     /gene="rimI"
                     /locus_tag="IWJ07_002683"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP9334201.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(2552..3376)
                     /gene="tsaB"
                     /locus_tag="IWJ07_002684"
     CDS             complement(2552..3376)
                     /gene="tsaB"
                     /locus_tag="IWJ07_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAP9334202.1"
                     /translation="MYHCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDKIIVPMFDARRKNVYAGAY
                     RWKENRLEIVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFYEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
BASE COUNT          956 a          745 c          591 g         1109 t
ORIGIN      
        1 tttatcaggg tcactgctta ttttcctccc taaaacttct gaaagatccg gcagttttag
       61 atcactgggc aagactcaaa gattttctat gtgtgtttca gttatgtaat attcactgtt
      121 tgcaaaaaat actgatagat taactagagg aagcgtcttg tcctcatccg acctagatta
      181 ttcgatattc ctcgacaata aacgattaat cagtgagggc aatcagagga acggataatg
      241 atgaataagc tgtaaaaaat agggctttga caaaagcgtg tcacagccct tatttcgatt
      301 aaacggtgtg tacctgtcaa ctcttcggtc acaagtcata atgttcataa acatgaggag
      361 ctgttttttg aaattgttcc ttgtgaatca gagttaaacg acaggcttac gaactcttga
      421 ttaaacggcg ttcaaaagct tgcatacgcg gtaataagaa cagccaggtc aaaaacatga
      481 gaagccgttt ttcctgggct gttcttatta cttgatgcag ggcagatttt tcacaacctc
      541 tttattcaga taaaaccaag cttggatcag cattcaactg atagctagca aaatttcttt
      601 tttgcatttc cacatgtgct gctgctccaa tcattgcagc attatctcca caaagtcgaa
      661 gagggggaat cactagttct acctcaggga gtttagttgc caaagcatcc tgcaatcctt
      721 cacgcaaccc ttgattggcc gctacacctc cagctacaat cagttgcttg actggataat
      781 tttgacaagc tcgaagtgtt ttagtaatca atacatcgat cacacttgct tgaaagctag
      841 ctgctaaatc atttttgtct agatcttctc cacgctgttg tgcattatgc accaaattga
      901 taaatgcact ttttaatccg ctaaaactga aatcataatt atcttcatgg atcatcgctc
      961 gtggaaaatg gtaattatct tttccctgat gagctagctg atcgatttct tttccgcttg
     1021 ggtaagaaag acctaatact cttccaactt tgtcataagc ttctcctgct gcgtcatctc
     1081 ttgtctcccc aatgatttca tatgatccat cttcttgcat atagactaat tctgtatgtc
     1141 ctccgctaac aagaagtgcc atcaatggaa actggaatgg tttaaccaga cgggctgcat
     1201 agatatgccc tgccatatgg tttacaggaa taagcggcaa ttggtgtgcc caagcaaaag
     1261 ccttagctgc agaaatacca atcagcaacg agccgacaag tcctggtcca taagtgacag
     1321 cgactgccga taaatcttct gccgacacac cagcttctac cagtgcatct tccaagcata
     1381 aggtgatttg ttctacgtga tggcggctag ctacttcagg aactacgcca ccaaaacgtt
     1441 tatgactgtt tatttgagaa gcaacgatgt tagataagat ctctgttcca tttctaacga
     1501 ccgctacact cgtttcatca caacttgatt caatcgccag aatcaattct tcattcattt
     1561 catcgtctcc gtttttaact ttctgctcat aatgatcgca tcttccgtcg gattgcgata
     1621 atatgatttc cgtttaccta atatacgaaa cttttctgat tcataaagtt ttcttgctgc
     1681 ttcattagat agacgtactt ctaaaaaaac atgcagcgaa tcttttattt gttccgtaat
     1741 cagtaattgt aacaaatgcc gtgcatgccc ttttccttgt tctgaaactg caacagcaat
     1801 atttgtgatc tcgacttcat ctattacttt cgagtatcca ataaagccac agatctggtt
     1861 atcttcaact aaaagcagat aatcagagtg aggctgttgg atgtctgcca aaaactgcat
     1921 tttcgtccaa ggtgaaccat gtggataagc attttcgcta atcatccata cgcattctgc
     1981 caagtacgct tcatcaaaat ctttttttct gcaaatcaac taatctcact cattttctaa
     2041 aagttttatc atatccaacg catcttcttt tgtttcagtg taatatcttt ttttgatcgt
     2101 tgttgcttga aaacctaact tccgataaag tctttgtgca tcttgattac tcatacgcac
     2161 ttctaatgac atcgtctcac agcgattcat tatagcaaag ttctcgattt cgtcaataag
     2221 cagcgaaccg attcgtttac gctgatatgc cggtcgaacg gcaatattcg tgatatggca
     2281 atctaaaccg aacgctcttg aaccgataaa accaatcatt cttccattat caaagatacc
     2341 tagatataga tgaggaagcg gtgacttcaa ttctgatata aaggcacttt ttgtccatgg
     2401 caattcgcca ttatagactt ctcgttcgac atccagcagc tctttgatat cttctgtagt
     2461 caattctctt agtacgtaac gacgatcgat ttgttcgatt gttttttctg tataaatcat
     2521 tttatcttga aaaaagcttc tggcatacgc tctaaatttt ttcaacataa ctttcgtctc
     2581 caggtgtgtg cgtctccaac catttttctt ctgcttctac ccgttttaaa tagcgaggaa
     2641 gaaaagcttg tttctctgca actggttcag ctaaagcacc taattgagcc acagttacac
     2701 cacttggaat atcccatgct ggaactgtat taatcatcgc actaggcaat atttccttta
     2761 tttcatagaa gaatttgtga caatcactcc cgacgaaata aatagcttca ttcagctttt
     2821 gtagttgtgt gaacaaatca gaagcagaaa tatgtacatc ttcaagtaca atttctaatc
     2881 tattttcttt ccagcggtat gccccagcgt atacattttt tcttcgagca tcaaacatgg
     2941 gcacaataat tttatccaca ccgatgcaat tagccgcgat ggttttcaga ctagatactc
     3001 ctactagctc tttatctaat gtataagcaa tcgtttttgc tgtcgttaca cctatacgaa
     3061 gccctgtata agaaccggga ccatcagaaa ccgcgattcg gtcaatgtct tttggactta
     3121 tttgaagatc tgcaaacaat tggtcgattg caggcatcag tgttacactg tggttgcgtt
     3181 tgatatttgt ttgtatttgt ccaagaattt gttcgtcttc taccacaccg atcgccaaag
     3241 tttggtttgc agtatctatt cctaatgtaa tcatatattt gatcctttct gctatgacat
     3301 tctcgccttc cattgtagca tactttttta ttctgaggat gaaaatacca aaggcaaaac
     3361 tagtacaatg atacatgatt atctgctggc gggcaaattt t
//
LOCUS       DACDPS010000151         3389 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.154, whole genome shotgun sequence.
ACCESSION   DACDPS010000151 DACDPS010000000
VERSION     DACDPS010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.154"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..2303)
                     /locus_tag="IWJ07_002685"
     CDS             complement(<1..2303)
                     /locus_tag="IWJ07_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334203.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAIDGLAPGAYQLVET"
     gene            complement(2975..3243)
                     /locus_tag="IWJ07_002686"
                     /pseudo
     CDS             complement(2975..3243)
                     /locus_tag="IWJ07_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          921 a          763 c          548 g         1157 t
ORIGIN      
        1 gtttctacca gctgatatgc tcctggtgct aaaccgtcaa ttgccagttt tccatcttct
       61 cctgtagtta aaccggtttg tagcgtttct ccttctcggt tctgtaactc aaaaacagct
      121 ccttgaagga cttcaccact gccttcagca tgtttaatta gttcaacacc acccgtatca
      181 aggatgtttt caatggttaa cgtcacttct tctatggagt attcatttat aaaaacatca
      241 tgtaccgtcg catctaaaag atacccttcc ggtgcttctg tttctcggac ttgatagcta
      301 cctacaggaa gcggttcact taaaaattct ccgttttctg ccgttatacc ttcttgtatc
      361 acttgtgaat cgcttgataa aatttcaaat tttgctcctg ccagcctttg gccattctca
      421 tcagttttaa ttactttaat acgcccatcg attttccttg ttcttacagt actcgtattc
      481 aatcgattgt cactattcac aagcgttgca aagtttaaaa tttgatcgcc gaccgcatac
      541 gcctctacat tgaccttaaa agagagggta aaacttgtcg gaccaccaat cgtattattc
      601 gcactgaaac gaactattcc attattgact tcggcatcaa tgaaagaggg aatatctgat
      661 agaaatgaca caaattccat gccttcttcc aaaacatctt caataacaag cgtatttctt
      721 gttccagaaa acatcgagac atgcagagta tacgttagtt cattttcatt gagattgata
      781 acatttttgt ccacactttt tcttaaagag attgccggaa gattactttc agggattctc
      841 aagcggtagt ctcggctcat tacctgatta ttgtaggtca tactcgctcg attccgagaa
      901 aacccatcct cttgtcttcc ttcattgaac aagtcgacat cggtcacttc aagagcatat
      961 tcaataaaat acgattgacc ctctgctaga ttaccaaagt ccacaacaag tgtattcgtt
     1021 tccttattaa aagatacttt gtccacaaat tcctcggaaa cataatgagt tctaacgttt
     1081 gcttcacctg gattcgttac ttctgcgatc cgaaccccac ctgaaatagg ttctctattt
     1141 ccagtggaac tccatgtagt cggattcggt gccaaagttg ttccttgcgg tagtacatcg
     1201 gtaaccacta caccgtcttt tcgtgatcct tcataattta cctgcaaaat aaatttatta
     1261 ctgttggtca cgtcttgccc gtcaatcagc agattcccaa gatttccttg gctgtcgatt
     1321 tctctagagc catagtataa tttcgaaaaa atgggtcttg atgaaggaat aggcgtaatt
     1381 gaaaattccg tgtttgctgc tcgctcagca taagtaagac tcacttgaaa gtcattattt
     1441 ggctcagtac cgcctgattg aaagaaagca acttcaaacg tgatactgaa tgtatacgac
     1501 tgagcggctt catttaatcc ttcataatca aaggtaaatg gaaaaacaac gtctgtgcct
     1561 tcttggtaaa atgccagtgg ctttaaaggt gaatcatcag cgacctccac tctaatgaac
     1621 tcaattaagc caatcgatgc tctaggaact cgtacgacca tctccccata ttcttcaaga
     1681 atatcgccag cgcctccttg aatgtatccc tttaacgttt gtgtatgcct gctcggcaaa
     1741 gtggggcgct cagcagagat gccgatgtca taattcaaga gctcctcttg tctttcaatc
     1801 tgagattcct gcgttataga tggctcagat gatctggctt cattaaaaga ttcacttaag
     1861 ctttcttctg gtatattaaa agattcactt gtgctttctt ctggtacatt aaaagactca
     1921 cttgtgcttt cttctggtac actgatttct attgagaccg tatttgattg aatatcaccg
     1981 cttcttgcgg tcaattcgta tttacctgaa tgctgggaag aataagtaat cagacgttcg
     2041 taatcattga gttgcttcac ttccccttca atcaaatcct cactcttatc aatgaccaat
     2101 gcatttagca ggtctggtgt tcctctgact cgcaactgga actgctcacc caaagcagcc
     2161 tcctcttgat caacttcgat cgtcagcgaa cgctctggct gttctgctaa agactcagct
     2221 agaaccgtta tgttcccaaa ttgattgatc acaataagca taactagaaa catacttaac
     2281 agtttatgaa atttgttcct cactattact cccccttata attttgacta aacaacaagg
     2341 tacccgataa ttttctaaaa agattcccca actcaaaatg gctgcgttac ctccctcgta
     2401 ttttcggttc aactcaagcg ttgcaatgta agctgtaaac aaagctaatg attgccgatt
     2461 gtcttctgtt gattgattcg atagctttgt atgactttta gttgtccgtg tggcttaacc
     2521 ataattatga tttcacgatt cctcctgcca gtgccttgca caagctggag cgtaacccta
     2581 atagttattt tgttaatgta cttatatact tttatacttt tccattagaa acaatcacta
     2641 aatcgttgat accaaggtca gactctagaa tttcatcagt gagtccctgt attggttaac
     2701 cttcatcaat cccaaaaaaa tatatcaatg cttagcatca aaatgaatat atagtttaga
     2761 atacttggga ttcatcgata actgatcgct tatttggcca tattatttgg cacttggttc
     2821 aactaaacgc atttaggatg ttccttcatt ctcgtcatgg atgttctcaa accgtcatta
     2881 ctaataaact gtccaccgaa tcgatggttc atttcttgca ttttccacgt ccaattactc
     2941 tttttgctct ttatttgggc gtagtttcac tttcttaact tgtatcgtct tccatcaact
     3001 ccttaatcat cttcttagct ttattggctc ggtttccttg taaaaggtat ctacaaatag
     3061 tgacaatctg tattaaatct tcatcatttt ctgtaatgtc aataatttct atattctgta
     3121 ccatatattg caaagggttt cactaagttc ataaccgaag cgaattaaac aacttttgta
     3181 tagaataact accttttcta tttttccatt ggtaattcta tctattaatt ggtttaatcc
     3241 ttttgttgta gttgattcca ctaccaagat gattctctcg aattttaaag cttgaagatg
     3301 tattgctatt tttgctaact ggcgtatttg caactatttt tcccttctat tcttccttca
     3361 ttgattcccc tctaaaatta ctatccata
//
LOCUS       DACDPS010000152         3341 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.155, whole genome shotgun sequence.
ACCESSION   DACDPS010000152 DACDPS010000000
VERSION     DACDPS010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.155"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..289
                     /locus_tag="IWJ07_002687"
     CDS             <1..289
                     /locus_tag="IWJ07_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9334204.1"
                     /translation="FIPRLGIIAAGYTTVFTYFLLFIFHYNIAKKITNKQLFDTKKIC
                     IIIIFVSIFSLILHILINQMLIRYVLTSLLIISLVIYFLKIKNRIIDDYNS"
     gene            302..1309
                     /locus_tag="IWJ07_002688"
     CDS             302..1309
                     /locus_tag="IWJ07_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9334205.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1321..2325
                     /gene="neuB"
                     /locus_tag="IWJ07_002689"
     CDS             1321..2325
                     /gene="neuB"
                     /locus_tag="IWJ07_002689"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HAP9334206.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2322..>3341
                     /gene="neuC"
                     /locus_tag="IWJ07_002690"
     CDS             2322..>3341
                     /gene="neuC"
                     /locus_tag="IWJ07_002690"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAP9334207.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFI"
BASE COUNT         1251 a          426 c          614 g         1050 t
ORIGIN      
        1 tttcatacct aggttaggaa taattgctgc tggatacaca accgtattta cgtatttttt
       61 actttttatt tttcattata atatcgctaa aaaaattacg aataaacaat tatttgacac
      121 aaaaaaaata tgtatcataa taatttttgt atcaattttt tctttgatac ttcatatttt
      181 aatcaatcaa atgttgattc gttacgtttt gacatcatta ttgataatta gtttagttat
      241 atatttttta aagattaaga atcgaattat agatgactat aattcatagg aggagatata
      301 agtgtttaaa ggaaaaacaa tattagtaac tggcggaact ggttccattg gaagtgaaat
      361 tgttcgtcag cttttaaaat atgaaccaaa agctatcaga atttatagta gaggtgaaga
      421 aaaacaattt tatatgcaac aagagctctc aaatcacact aatttgagat ttttaattgg
      481 tgatatcaga gactctcaaa ggattgatta tgcatgtcgt gatgtggata ttatttttca
      541 tgctgccgca atgaaacacg ttccagcaag tgaatataat cctatggaag ctgtaaaaac
      601 aaatgtgatg ggaactcaaa atgtaataga tgcagcgatc aaaaataatg taaaacattt
      661 tgtaggtatc agtacagata aagtagtgag tcctactaac accatgggtg cgacaaagtt
      721 attgagtgaa aaattaataa tagctgccaa tcagtataaa ggaaaaacag aaacaatatt
      781 ttcttgtgta aggtttggaa atgtcatggg gtcaagtgga tctgttattc ctctttttag
      841 taaacaaata aaaaacaata aagatattac tttgacagat gttgatatga cacgatttat
      901 gatgtcaata ccggatgctg caagattagt tctgtctgct gctatgaatt caaaatttgg
      961 agaaacatat gtcttaaaaa tgccggcaat caatatttta actttagctc aatcattaat
     1021 aaaaatatat aatgatacac atgatgaaaa atatagaggt gaaataaaag ttgtaggtat
     1081 caggccagga gaaaaattat atgaagagct aatgactaca gacgaaattg aacgctcata
     1141 tgaaaatgat aaaatgtata tcattccacc acactatcat gctactgacg aactgtacaa
     1201 agattatgaa aaaattaaaa tcactgcata ctcttcagat gcaacagaat taatgagtgg
     1261 acaggaattt attagaatcg caaaagagaa taatgtcgtg acttggtaag gagttttatg
     1321 atgaaatcag aagtatatgt aattgcagaa attggtgtaa accataatgg aagtctagat
     1381 ttagctttag aaagtattga taaagctaaa gaatgtggag ctaacgcggt taaatttcaa
     1441 acatttaaaa cgaataagct agtttccaaa aaggctccaa tggcaaaata tcagaaggat
     1501 aatcttaaag aggacagttc tcaatttgaa atgctaaaaa aattagaatt atctcaatct
     1561 gattttatta gaataaaaga ttattgtata caaaataaaa ttgatttttt gtctactcct
     1621 tttgatgaag aaagtgcaga ctttctaaaa caaattggtg tacaaggatt taaaattggc
     1681 tcaggagatt taacaaattt accttttctt agaaaaatag atatgtatgg gttgccagtt
     1741 ttattatcta caggtatggc tgatattcat gaggtcagtg aggctgtaga ttgtttcata
     1801 gatagtccag ttactatttt acattgtact tcaaattatc cggctagttc tgaagatatt
     1861 aatttattgg cgattaacac attaaaagaa atgttcggga tacccgttgg gtactccgat
     1921 cattcattgg gatatgatgt tggtatttgt gctgttgcga tgggtgctaa ggtaattgag
     1981 aaacatttta ccttggataa ggaactacct ggtccagatc ataaagcttc tttagatcct
     2041 caggaatttg cagattttgt aaagcatatt agaaacactg aaatattttt aggtgatggt
     2101 gttaagcgag caatgccttc tgaacttagt acaaaagaag tatcaagaaa aagcctagtg
     2161 gtcactgaga atttaaatgt gggagatatt cttacagaga aatctcttac aataaaacgt
     2221 cctggaaatg gaatagctcc taagttttta tatcaatata ttggcaaaaa agttaaaaga
     2281 ccaataagtg cagatagtgt tctgcttgag gaagatgttg aatgaaaaaa atacttatag
     2341 taagtggaac acgagctgat tatggaatat ataaacctgt tgctgagaat ttactacaag
     2401 ataattggga tgtatcattt gttgtttctg gaatgcattt atcccataaa tatggattta
     2461 ctaaagaatt gatcttagct gacggattta gaattagtgg agaagttcaa agtttgtttt
     2521 tagagaacaa taagggaaac atggctcggt ctgtggcatt agagataaat ggtttcacaa
     2581 atatatttga acaaactagc cctgattttg tattattact aggggataga ggagaaatgt
     2641 tggcagctgc gatagcttgt ttgtatcttg gaatcaaaac agcacatatt cacggaggcg
     2701 aagtgtctgg tactgtagat gaatcaataa gacatgccat aacgaaaata gcatctgttc
     2761 attttgctgc tacggaaaaa agcaaagagc gtttaataaa aatgggggaa gattcttgga
     2821 gagtttttcc cgtcggagct cctagaatag atactatttt aaataataga ttacctagtt
     2881 ttagagatat aaaagaaaaa tatcatctaa aatttgaaaa gaaaaaatac gttttgcaag
     2941 tatttcatcc agttgtaacc gaactagaaa atatagaaaa acaaatttta gctttgatca
     3001 attcaatgga acaacaatct caaccaatta tttgcatttt acctaatagt gatgctggta
     3061 gcgaaattat cagaaaaatg taccagaaat atttaaaaac ggatattatt tatattacga
     3121 atttagagcc ggaggaatac ctgactatat taaaagagtg caaatttatg atcggaaatt
     3181 cttcttctgg gataattgaa gctgcatcat tcaaaatacc agtaataaat ttaggaacca
     3241 gacaaaatgg acgagaacaa tctaaaaata ttataaatgc aaatctaaat gaaaatgaaa
     3301 ttgaaactgc tttgaataaa atacaagatc ctcagtttat a
//
LOCUS       DACDPS010000153         3317 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.157, whole genome shotgun sequence.
ACCESSION   DACDPS010000153 DACDPS010000000
VERSION     DACDPS010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.157"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(554..2167)
                     /locus_tag="IWJ07_002691"
     CDS             complement(554..2167)
                     /locus_tag="IWJ07_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAP9334208.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2308..3216)
                     /locus_tag="IWJ07_002692"
     CDS             complement(2308..3216)
                     /locus_tag="IWJ07_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9334209.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
BASE COUNT         1080 a          564 c          578 g         1095 t
ORIGIN      
        1 tgttaaataa ataggaacaa aaaaattaaa ttatttaata cacacattag ggatcattca
       61 ttgaaggatt agaagacaac gaggatggcg aaaaatatga ttacaataga aaagtcgttc
      121 taaaccaatc agttcctagt atcacacaac gcaagccagt cgatttggtg agattagagg
      181 agtgaaagca aactttatta tataagaaaa aatattcgaa agggatgcta gtttaggtac
      241 tagtgctgat agattaatat gatgcctcgt aatattaggc gtagttactc tagagacatc
      301 actatggact atgatttgtc tcagagtcac aacatgtttt tttatgaaaa gaaacagcta
      361 aaataattaa attgcgcaat gtttaaactt tcttaaacta taacctaata aatgtgaaga
      421 aataatgcac aatttcttca catttatctt ctattctata tacataccaa ccaacaaccc
      481 tatatacttt ttcattttta ctcctcaagt aaaaataact cctagaccgc ggctacccac
      541 cgcggtcgtt tttttattct tcaatcaaaa tcgctttgat acccaaacct gccaagattc
      601 gggcagaagt ataagtattc aatcctcttc tgaaagtaat gtaaactttt ttatcttttg
      661 ggatttcatt gattcgatct cgtaactcat tcattggaat attttttgtt gccgagatac
      721 tgccagaagg tgctttatta ggttcacgta tatctaaaaa gaacgcttct ttcaaatcgt
      781 tttcgggtat atcacttgct ttgatcgttt ctacgatatt cgtcatttga ttgattgcga
      841 cataaccagc tatattcaac gggtcacggg tagtagaata cggtggagag taaggcaact
      901 ccaaatcagg caaatcaaag actgtcaagt ttccggtgat tgctacagaa agttctccca
      961 tccgtttatc gacacctttt tctccaaatg cttgtccacc taaaatacga ccgctatctg
     1021 catcaaaaat cagctttaga tttaatcttg tagcttctgg atagaaatag gcatggtcaa
     1081 agggagtgat aaaaacagtt ttataatttg ttatgcctga tgcttttaat tcatgttctg
     1141 tcattcccgc ataacttgct gtatgatcaa agatttttgc gacacctgca ccaatatatc
     1201 cgcgatagcg cataggcgta ccatttaaaa tatctgccag catatgtcct tgacgattgg
     1261 ccgcactgga aagcatactt tggatcggct gaccagtcac tacactagtc gtttcgataa
     1321 tatcgccaat cgcatagatg tccggcagat tggtttgtaa ctggtcatca acaatgatct
     1381 gacctgtatc tgataattgt atgccggttg ctttcaccac ttcattcttc ggagaaacac
     1441 caacagcaaa aattagcata tcagcagaaa ggacggagcc atctgataat actatttctc
     1501 gacctttatc acggatctca gtaactcttg tttctaaatg aacggctaat ccttctttca
     1561 gcaatttgtc ataaactaaa ttagcaattt ctggatcata agggaaagcg acttgtggca
     1621 attgatcgat taaagtcacg tctaagccgc gatgcttgaa attctcagct aattccagac
     1681 ccataacacc tgcaccaaga atgacgacat gttgtggatt tttttcttca aggaacgatt
     1741 tgattcgatc cgcatccgtc acactacgga ggacaaagcc gttttctgct tcaaaaactc
     1801 cgggaatatc cggataatct ggtctagcac cagaagagat gatcaactta tcgtaatctg
     1861 tttttatttc ttcatttgtg gataggtctt taactgttaa tcgcttagta gaaggatcga
     1921 ttgctgttac ttcgtgttta gtaaagacat cgatgttgtt ttttgttttc aatatttctg
     1981 gtgtacgttc gatcagcgag tcacggtcag tgatcacacc tcctaaataa taaggcaaag
     2041 cacaacttgc ataagaaata ttttctccgc gttcatagat aatgatttcg tgcgcttcat
     2101 tcaatcgacg gaaacgagtg gcaaatgaag gaccgccagc tacaccgcca atgacaacaa
     2161 ctttcataaa aacatctcct tttctacgtt gatcgtacta tgttatggta aaaaacagca
     2221 aataattgtg ccggaaaatt attgtttatt ttgaatagac agaaaatata gagaaataat
     2281 ttgtggataa aacctaaaaa tttttttcta ttttaaggat agataatact ttgaagggga
     2341 taaccctgta tattttttaa aagctgaaga aaaatgatgg atcgaatgaa atcctaatat
     2401 atctgcaatt tctgtatagg ttaattcatt ttctagcacg agacgttttg ctttttccat
     2461 ttttagttct ttaaaatagc taataacact ttttccattc acttttctaa aatgtttttt
     2521 taagctggct ggactgataa aaaaaatttg ggctaattgt tcaagcaaaa aattttctga
     2581 aagatgttct tctaaatatc gtatgattgg atcaactaat tcatctgaat aaagaatatg
     2641 ctttttttta ggaatgactt tctcatttga aggtattgtc aataaagcaa gttcctgttg
     2701 ataatgcctt ggtaaggaag acaattgaat tttttgcaga caataagtaa ctaataagga
     2761 aagcaaatga atgctcatct gttggcatag tatgaaaaaa gaattgattt cctgatcata
     2821 ggaaatagct ggcttactat gttttagata atcaattttg aactgttcat ttttcagaaa
     2881 agtaatgata gatgttattt cttgatctat agaaaaagat tgtcccgtaa tatgtgctaa
     2941 taattgctca tcaaaataaa actggataat gataaaagtt tgtgtttcct tttgagtaga
     3001 aaagatttgg acttcttgct gtttatcaat gaataaaagg gaaggattat ctagttctat
     3061 agatttatgt gcaaattcag catataaatt agcgctttca tttattagta gtcgaaaata
     3121 gggagcagca gataaatgaa gatgggtatc tatggaatag gttagtgttt ttttgacaag
     3181 attggaggat tggttgtttg ttaacaaaag aaccactttc ctttctcttt ttatataaaa
     3241 agctttctat ttataattaa tgactattat aacaaaaatg aaaagccaaa acgacaagta
     3301 aaatattttt ttatata
//
LOCUS       DACDPS010000154         3311 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.158, whole genome shotgun sequence.
ACCESSION   DACDPS010000154 DACDPS010000000
VERSION     DACDPS010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.158"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(451..1761)
                     /locus_tag="IWJ07_002693"
     CDS             complement(451..1761)
                     /locus_tag="IWJ07_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP9334210.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3191)
                     /locus_tag="IWJ07_002694"
     CDS             complement(1773..3191)
                     /locus_tag="IWJ07_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9334211.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
BASE COUNT         1020 a          673 c          512 g         1106 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct ttttttattg tatttgctgt aacagcattt
     3181 tgtgaaagca tttgaattcc tcctacctct tctagtaaac gttttcaaaa ttataactct
     3241 ttttttcttg tttttatatt cacaatgttg gctttttatt gcattatctt gacagataaa
     3301 aaacaaaaaa t
//
LOCUS       DACDPS010000155         3225 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.159, whole genome shotgun sequence.
ACCESSION   DACDPS010000155 DACDPS010000000
VERSION     DACDPS010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.159"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            31..1683
                     /locus_tag="IWJ07_002695"
     CDS             31..1683
                     /locus_tag="IWJ07_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAP9334212.1"
                     /translation="MDHNANYYHWLTNANAEIVEELRSYSEKDIEDSFYKNLSFGTGG
                     LRGTIGAGTNRMNVHTVGKASQGLSDYLNKTYETPSVVVGYDSRIKSDVFAKVAADIF
                     AANGIQVHLWPELLPVPTVSFAIRYLKASAGVMITASHNPSKYNGYKVYGAGGCQITT
                     EAAAEILAEIEKLDIFADVKMGGDASRIASIQPEVLTAFIEEVKGQSVLFGEEVDKNV
                     AIVYSPLNGTGLVPVTRTLAEMGYSNVTVVEEQRLPDGNFPTCPYPNPEINEAMSLGM
                     EYAKKYNADLLLATDPDCDRVGIAVKNAEGDYVLLSGNETGLLLLDYICSQRIKHGKM
                     PTDPVMVKTIVTMDLGERIASNYGVRTVDVLTGFKFIGEQIGMLEKAGKVDSYIFGFE
                     ESYGYLSGSYVRDKDGVNAAYLICEMFSYYKTQGISLFDKLNEIYAKYGYCLNTLHSF
                     EFEGAAGFTKMQKIMVEFHKGLDMIGGKKVIETLDYSTGLNGLPKSDVLKYLLEGNCS
                     VVVRPSGTEPKLKTYISVSAESKEAAEVMEAVMAAELAEMFK"
     gene            2027..2392
                     /locus_tag="IWJ07_002696"
     CDS             2027..2392
                     /locus_tag="IWJ07_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HAP9334213.1"
                     /translation="MYYFQQSYLPAILNKAISIGDTGVHIFFFCSGFGLYLSYLKKPI
                     TLGEFLQKRFIKIYIPYIIVITVSVLLPYMTQDLNAAKVYLSHVLLFKIFLPVYEESL
                     GPFGLFRQLFSSISYSYQL"
     gene            2480..2923
                     /locus_tag="IWJ07_002697"
     CDS             2480..2923
                     /locus_tag="IWJ07_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334214.1"
                     /translation="MEAYEFLGAALRNIFGNLLSACLVAEYLVKRKIVRFNNIFVIMA
                     SIVGIGFAGISKIIGGLMESINDIFAFFGYGAIAILMYQVIPFRKIMPRISRYSYDIY
                     LLHILVFSTIHQIIPNLVLAAFVSIPITLVLSYYYSIMLYRLKLK"
     gene            complement(3078..>3225)
                     /locus_tag="IWJ07_002698"
     CDS             complement(3078..>3225)
                     /locus_tag="IWJ07_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP9334215.1"
                     /translation="EKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTLRY
                     SLGY"
BASE COUNT          906 a          580 c          753 g          986 t
ORIGIN      
        1 agcagaattt taaaagattg gagagattct atggatcata atgcaaatta ttatcattgg
       61 cttacaaatg ccaatgcaga aattgtagag gaactgcggt catattccga gaaggacatt
      121 gaggactcct tctataaaaa tctgtccttt ggtaccggtg gtctgcgtgg caccattggt
      181 gcaggcacta atcgtatgaa cgtacacacc gtaggtaagg caagccaggg actgtcagac
      241 tatctgaata agacctatga aaccccttct gtcgtcgtgg gctatgattc ccgaatcaag
      301 tccgatgtat ttgcaaaggt tgccgcggac atctttgctg caaacggtat tcaggtgcat
      361 ctgtggccgg agctgctgcc tgtgcctacc gtatcctttg ctatccgtta tctgaaggct
      421 tctgccggtg ttatgatcac cgcatcccat aatccttcca agtataacgg atataaggtc
      481 tatggcgcag gcggctgcca gatcaccacc gaggctgccg ctgagattct ggctgaaatt
      541 gaaaagctgg atatttttgc agatgtgaaa atgggcggcg acgcttccag aatcgcttct
      601 atccagccag aggttctgac tgctttcatt gaagaagtaa agggccagtc ggttctgttt
      661 ggcgaagaag tggacaagaa tgttgctatc gtgtattctc ctttgaatgg taccggtctg
      721 gtgcctgtta cccgtactct ggcagaaatg ggctacagca atgtgaccgt tgtcgaagag
      781 cagcgtctgc cagacggtaa tttcccgacc tgtccttatc cgaatccgga aattaatgaa
      841 gctatgtctc tgggcatgga gtatgccaag aagtataatg ctgatctgct gctggctacc
      901 gaccctgact gcgaccgtgt gggtatcgcc gtgaagaatg cagaagggga ttatgtcctg
      961 ctgtccggta acgaaactgg tctgttgctt ctggactaca tatgctctca gagaattaag
     1021 cacggcaaga tgcccactga ccctgtgatg gtcaagacca tcgtgactat ggatctgggc
     1081 gagcggatcg cttccaacta tggcgtgcgt actgtcgatg tgttgaccgg cttcaagttc
     1141 atcggcgaac agattggtat gctggaaaag gcaggcaagg tggacagcta tattttcggc
     1201 tttgaggaat cctacggcta tctatccggc agctatgtcc gtgacaagga tggtgtcaac
     1261 gccgcttatc tgatctgcga gatgttctct tactacaaga ctcagggtat cagtctgttt
     1321 gataagctga acgagattta tgctaagtat ggctactgtt tgaacaccct gcattccttt
     1381 gagtttgagg gtgcggcagg ttttaccaag atgcagaaaa ttatggttga attccataaa
     1441 ggtcttgata tgattggagg taagaaggtc attgaaacct tggattacag cacgggactg
     1501 aatggcctgc cgaagtctga tgttctcaaa tatctgctgg agggcaactg ctctgtagtg
     1561 gttcggcctt ccggcactga gccgaagctg aagacctata tcagcgttag cgcagaaagc
     1621 aaggaagctg ccgaagtaat ggaagctgtt atggctgctg aactggctga aatgtttaag
     1681 taatactatg aaaaagatct gagtgtcaat gtccgaagta gcaacatcta tgtttaataa
     1741 aaatttcctg ctttacggtt ctgtttacat aattgaggta aaggggccga ctgtgtggat
     1801 cgaaaaggca aagaagtgtt aacagagaca cagcagcctg atgaagcacc gatcaaagca
     1861 gtaagggaga aataagagat attagagttt taataatgta ttcagcaata gagatgaaag
     1921 gagtagaata tgaataaaac tattgaaata ataaaatgag ggggaaagaa ataaacgtga
     1981 tattattagt tttgagaagg cgttttcaat attcgctatt gtattgatgt actattttca
     2041 gcaatcgtat ttgccagcaa tactgaacaa agcaatatct attggtgata caggagtgca
     2101 tatattcttc ttttgctcag gttttgggct atatctgtca tatttaaaaa agcctattac
     2161 attgggtgaa tttttacaga aacgatttat caagatttat attccgtata ttatcgtaat
     2221 tacggtatca gtgttactac cttatatgac acaagattta aatgctgcaa aagtatattt
     2281 gtctcatgtt ttgttattta aaatttttct accagtatat gaggagtcac tgggtccctt
     2341 tggtttattt cgacaattat tcagttctat ttcttattca taccaattgt aaaactatag
     2401 agaaaaatag gaatgaaatg attgcttgtt gcaagctgag ttattagtat ctgggctatt
     2461 atgatgtttc atacaggata tggaagcata cgagttcttg ggagctgctt tgcgcaatat
     2521 atttgggaat ttactttcgg catgtttggt tgcagagtat ttagtgaaaa gaaaaatagt
     2581 aagatttaat aatatattcg tgattatggc gtccattgtt ggaattggtt ttgctggtat
     2641 atcgaaaatt attggtggac tgatggagtc tattaatgat atttttgcat tcttcggtta
     2701 cggtgcaatt gcaattttaa tgtatcaagt tattccattt agaaagataa tgcctaggat
     2761 ttcaagatac tcatatgata tatatctact ccatattctt gtatttagta cgattcatca
     2821 aattatccct aacttggttt tggcggcatt tgtttccatt cctataacgt tggtgttgtc
     2881 atattattat agtattatgt tatatagact taaattaaaa tgatagcata attgtcgcct
     2941 ctaccgggcg gttgccatcc tgattaacag cctccacgcg gcggatggtg ggcacttagg
     3001 tagttcgtaa cctagtggta gatggttggt aaaaaggaga taaaactaaa tgattacacc
     3061 cgtggtgcta gttaatttta atatcctaaa gaatatctca aagttccttc aaaacgtcgt
     3121 tgtgctcgac ttaatagaac actgtaagcc agtaatatgc tttcaaatct actctctagc
     3181 cctttaacag aacgtgagtt gaaattttga caacctaatt tttct
//
LOCUS       DACDPS010000156         3205 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.160, whole genome shotgun sequence.
ACCESSION   DACDPS010000156 DACDPS010000000
VERSION     DACDPS010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.160"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..202
                     /locus_tag="IWJ07_002699"
     CDS             <1..202
                     /locus_tag="IWJ07_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334216.1"
                     /translation="LFNLIKLDAKISSYLFFLIHDEFMDYQKLDQLIERESWEDYYVG
                     LTFSEKLNNYKLIDYKYLSESN"
     gene            819..>3205
                     /gene="esp"
                     /locus_tag="IWJ07_002700"
     CDS             819..>3205
                     /gene="esp"
                     /locus_tag="IWJ07_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAP9334217.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAGEGTSIESGTTVFAVKDGVS"
BASE COUNT         1172 a          472 c          626 g          935 t
ORIGIN      
        1 attatttaat ctgattaagc tagatgctaa aatatcaagt tatttattct ttttgataca
       61 tgatgaattt atggattatc aaaaattgga tcagttaatt gaaagagaaa gctgggaaga
      121 ttattacgtg ggtctaactt tttcagaaaa gttaaacaac tacaagttaa tagattataa
      181 gtatttgagt gaaagcaatt aattattcat gtagagtact agacacttta aatatggaca
      241 attcaattat aaagaaaaag aatcaataat ttaatgaggc tgtgacaaat gtcatttttt
      301 gtttcgctaa aaacgagtga aaaaatgttg ataactccaa tcattcgttt aagattctag
      361 gaatcattca gaaatgctgt gaaaccgata ctttataatt ataaagaagg tgatttcaaa
      421 ttccgaagaa ggaatttgaa gtcacctttt tacttatgtc acagtctcat tcagtaatgc
      481 gggataaggc tccttattcc gttatttgaa tactctgaag tttaatctaa gaaagggtaa
      541 tattttgaaa acatgcttca tcgcatagaa atgaacttaa tttcagaaag aatacataca
      601 tatcttttta tatgttttca gctgctgaat ggttaaaaag cacaaatatc cgaaaaaaac
      661 atgaagaaat gtactttttt tgttgtaatg ttcatgaaat tctctaagta aattagttaa
      721 cataaggtta gtaagattcc tattctgtga ataatacgta tcagatttat cttttattct
      781 tactaggaaa gtaattatta agggagttga tttgaataat ggttagcaag aataataaga
      841 gagtatttct agaaaaaaca aagaaacgag tattgaagta cagtatcaaa aaattaagtg
      901 ttggggttgc atctgttttg attggtgttg gtcttgtctt gggaacaact gaactagtta
      961 aagcacaaga tgaaatttct ccaagcagcc cacttgaaac agcgataagc tccgtacaag
     1021 taggtgacaa agtagcttcg gggaatactt ttcaggagaa tcctggttat acgaaaaatt
     1081 ataacttttc ggatttacaa tttagtcctc aagaactaac tggagacaca ctaaaaggca
     1141 atacaattgg atttgaagtt tacggaaaac ataatattgc agcatcaact aaaaactggg
     1201 aaatccgtct tcaattagat gaacgattag ctaaatatgt agaaaaaatt caggtcgatc
     1261 caaagaaagg aattggatct tctagacgga cctttgtaag aattaatgat tcgctgggta
     1321 gacctacaaa tatttggaag gtcaattata ttcgtgcaag tgatggatta tttgctgggg
     1381 cagaaacaac cgatacgcaa accgctccta atggggtaat tacgtttgaa aagagcctag
     1441 atgaaatttt taaagagata ggcatagaca atctcaagac cgatcgttta atgtatcgaa
     1501 tctacttggt aagtcatcaa gacgatgata aaattgtacc gggtattgat agtacgggct
     1561 actttttaac agattcagat gatttctata atagcttaga tgtatctgaa aataatcctg
     1621 accagtttaa acatggatca gtaagtgcaa aatatgagaa acctaatact caaacaaaag
     1681 atggttcagg atccacaggt gctaatggag ctataatctt agatcataag ttaacgaaaa
     1741 attataattt ctcatattca tcttccgcaa agggcacgcc ttggtatgct aattacaaaa
     1801 ttgatgaacg attagttcca tatgtagcgg gtatacagat gcacatggtt caggctgaca
     1861 aagtaacata tgatgtttcg tttgaatccg gaacaaaagt agctgattta gcaatcgagc
     1921 gacgggaagg tcatgaaaat tatggcatag gttcaataac taataatgat ttaactaaac
     1981 ttatcgattt tgctaatgca agtccacgtc cagtcgttat tcgatatgtt ttacaattga
     2041 ctaagccatt agatgaaatc ttagaagata tgaaagcaac agcacaagtt gaagaaaata
     2101 aaccatttgg tgaagatttc atctttgatt cttggttgtc ggatacgaat aaaaaattaa
     2161 tccagaacac ttatggaaca ggttattatt atttgcaaga tattgatggt gatggaaacc
     2221 ctgacgataa agaagagagc ggagacacga atccatatat cgggaaacct gaattagaag
     2281 aagtatatga tgttgacaca acagttaagg ggaaagtatt catccacgag ttagcgggaa
     2341 caggtcacaa agcccaactt gttgataaag aaggtactgt attagcagaa aaaactatcg
     2401 ctccaaatga aaaagatggg gctccaattt cagatactgt agaatttgaa tttacgggtg
     2461 tagattcaag taaattaatc gcgaaagatg aattaaaaat ccaaatcgtt tctccaggtt
     2521 ttgataaacc agaagaaggt tcaaccgtta ttaaggaatc accaaaagcg gttgataaac
     2581 aaaccgtggt agttggattt aaaccagatg ctaaagaatc aattcggaat aataaaaact
     2641 tacctgaaga tgcagagtat tcatggaaaa cagagcctga tacttctaac gttactgata
     2701 gtacgaaagg tattgtaact gttaagatcg gaaatcgaac tttcgacgtg gatgtagagt
     2761 ttgctgtaaa agcttctcaa gctatggaaa atgatgcaac atacgtacct ataacaacaa
     2821 ctccagaaac gacagttcaa agtggaaaac ctacatttga taaaccagat gttcctttag
     2881 ctaaagatgc cttttcaatt tcagatgtct ataataagga cttcggtaat gcaagtgttg
     2941 atgcaaacac tggtattgtt acattcactc cagctaaagg tgtaggagaa tcggagccga
     3001 ttactggaac aattcctatt aaaattgttt accaagatgg ttctgtaggc acgaccgatt
     3061 tagcagtaac tgtaagtaaa gatatttatg aaaatccagg agaaaacatt cctgcaggct
     3121 accacaaagt aaccttcacc gcaggggaag gaacaagtat tgaaagtgga acaacagtct
     3181 ttgcagtgaa agacggcgta agctt
//
LOCUS       DACDPS010000157         3155 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.161, whole genome shotgun sequence.
ACCESSION   DACDPS010000157 DACDPS010000000
VERSION     DACDPS010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.161"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..139
                     /locus_tag="IWJ07_002701"
     CDS             <1..139
                     /locus_tag="IWJ07_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP9334218.1"
                     /translation="AGIWGMNTGGLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYT
                     N"
     gene            217..1110
                     /locus_tag="IWJ07_002702"
     CDS             217..1110
                     /locus_tag="IWJ07_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9334219.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            1148..1711
                     /gene="amaP"
                     /locus_tag="IWJ07_002703"
     CDS             1148..1711
                     /gene="amaP"
                     /locus_tag="IWJ07_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP9334220.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            1725..1913
                     /locus_tag="IWJ07_002704"
     CDS             1725..1913
                     /locus_tag="IWJ07_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP9334221.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            1926..2828
                     /locus_tag="IWJ07_002705"
     CDS             1926..2828
                     /locus_tag="IWJ07_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9334222.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            2860..3102
                     /locus_tag="IWJ07_002706"
     CDS             2860..3102
                     /locus_tag="IWJ07_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9334223.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
BASE COUNT         1131 a          570 c          669 g          785 t
ORIGIN      
        1 cgcagggatt tggggaatga atacaggagg attaccgggt aaaagttctg ctctaggctt
       61 tatactcgta gtctttggct ctttgatagc agcagtagtt ttaggttacc atttataccg
      121 gaaagattat acaaattaaa gaatctattt aaaacaggta tcaaacaaag aaatttgttt
      181 tggcaataca atttaaaaac taggaggaat acagcaatga cagaaccaaa attaatcgac
      241 ccgcgaaact tgtaccatac ggagaaattt cctaaacaag atcaggatac accagcttta
      301 cagcagaata tgattcctgt tcctgattgt ggagaagaat cctatgaagg caataatcaa
      361 ctagaaaacc gccgtgtact aatcactggt ggagattcag ggatcggtcg tgcagcagcg
      421 attgcatttg ctagagaagg ggcagatatc gctcttcaat tcttcccagg agaagaaaaa
      481 gatgcagagg aagtagcaaa atacgtgaaa gttgcaggac gtaagattgt cttactacct
      541 tttgatcttc gtgacgaaca agcacctaaa gaaatcatag ataaagccgt gagtgaactt
      601 ggcgggctag atacgatggt actaaacgca ggacagcaaa tttcttgtca gtctattgct
      661 gagttaccac ttgagcaagt caaagacaca tttatgataa acatcatcgc tatgtttgct
      721 ttagtgaaag aagctgttcc gcatatccca gcaggaggag caatcgtaac aacaacttct
      781 gttcaagcat tcaatccaag cgaacattta ttagattacg cagcaacaaa agcttcaatc
      841 gctaacttta cgatcggttt agcaaaacag cttgcaccta aaggaatccg tgtaaatggt
      901 gtagcacctg gtccaatctg gacaccatta caactagacg aaggacaacc ttctgattcg
      961 atccctgaat ttggtcagca aagtttacta gaacgtgcag gtcagccagg agaattagca
     1021 ccagtttatg ttttcttagc atcgaataaa gcaagttatg ttacagccca aatttacgga
     1081 gtaactggtg gagaagcgat caacttataa gaagttgatc gtttcacccc tatgaggagg
     1141 tgcgaaaatg aataaaggtg taaaaacgat cggagtgatc ggaacattac ttttgctatc
     1201 tgtgcttttg tttgcgatac tattaagtgc aacctatcca ttgccttttc agctagagaa
     1261 gtttcgattc ttttccatca ctaattcata cgtgcaacaa tatgtatttt ggcttgccgt
     1321 tgcatttgct attttgacaa ttatcacgtt acttgttttc ctgttttatc caaaaacaat
     1381 cggaaacttt ctactgaaac atggtgatgg ggagttgacg ttggataaaa aagcaattga
     1441 aggaatggtt cgttctcact tgcatgaaga tgaattcatc cattcaccaa aagtaaatgt
     1501 caaagcaacg aaaaacaaga tcgccgtcaa tgtaaaaggg gagttgaaac gaacttcagc
     1561 actcattggt aaaacaggaa cattgatgaa agaaatcgaa aatgaagtac aacacatatt
     1621 gggaacaaaa gagccagtga aagtatcggt gaaatacaca ggctttgaag aaccagctaa
     1681 acgaagcaat gaccgcaaac gagtagaata gggggggacc aaaaatgaaa gaactatttt
     1741 cgacatacaa gctccctatc atctttgggg tacttggatt gatattagcc gttttatgga
     1801 tcaccgtggg ctttttcaag accttattga tcttgatcat ggcagcgatc ggcgtcgcta
     1861 tcggttttta tctgaaacaa acgaaaatct tagatcacta ttttaaatct taggaggaaa
     1921 catccatgaa taacaataac aaaaataata acaataaaat gaacaaccaa aaaccagtgt
     1981 ctgcacatcc agacccaacg acaccaaacc aatcaaatgg tgtgaacgat gaaaagaaca
     2041 aaacagcacc agtatctgca catccagatc cagtagtccc taacgttcaa caaggagtag
     2101 aagagaagaa acatgatgca gaaaagcatg cacctcatca tccaggaact ccagaatcta
     2161 aagttccaga acaacatgat gcaatcgatc cttctaaacc tgtggaagag caaaaacatc
     2221 cttctgacca tgcgaaagca caagaaaaac ttagttctga ccacacaaac ggacaaggaa
     2281 aacttggttc tgatcataca aataaccaag gaaagcatga ttctgaacag gaagattcaa
     2341 tgcgcggtga gctgaattat gaagataaag ttgtgcaaaa aatcatcgga atcgcaattg
     2401 aacaagtaga cggtttatta tctgcaaacg gtggtttctt ctctaatgtg gcaggaaaac
     2461 ttgtgaatac agataatgta acggctggga tcgaaacaga agtcggtaaa aaacaagtag
     2521 cagttgatat ggatgtcatt gtggaatatg gcaaagatat cgaaaaaatc tttgaagaaa
     2581 tgcaagaagt aatcggcaaa gaagtaaaaa atatgacaca tttagaagtg atcgaagtca
     2641 acgctaacgt agtggacatc aaaacaaaag aagaatttga aaaagatcaa gaaacggtgc
     2701 aagacaaagt aactgacgca gcgaaaaaaa ccggtgaatt tgcttcgaat caaacagata
     2761 aagtaaaatc agcagctgga agcggcgcac aaaaagtaaa agaaaataca gaaccacggg
     2821 ttcagtaata aggaagtaaa acattaggag gaacgaaaca tgggatttat ttggtcactt
     2881 atcgtcggag gaattatcgg agcaattgca ggagcaatca cgaataaagg ttcttcgatg
     2941 gggattattg caaatgtcgt agcaggttta gttggatcag cacttggtca agcattatta
     3001 ggttcttggg gaccaagtat tgcaggaatg gcaatcgtgc catcattgat tggtgccatc
     3061 attttagtac taatcgtatc attctttgct cgaaaactgt aaaaagactt ttaaaaagag
     3121 gtcgtgaaaa agctgttctt catcaagtaa taaga
//
LOCUS       DACDPS010000158         3074 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.163, whole genome shotgun sequence.
ACCESSION   DACDPS010000158 DACDPS010000000
VERSION     DACDPS010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.163"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(294..908)
                     /locus_tag="IWJ07_002707"
     CDS             complement(294..908)
                     /locus_tag="IWJ07_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9334224.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1514..1891)
                     /locus_tag="IWJ07_002708"
     CDS             complement(1514..1891)
                     /locus_tag="IWJ07_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334225.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2191..3072)
                     /gene="galU"
                     /locus_tag="IWJ07_002709"
     CDS             complement(2191..3072)
                     /gene="galU"
                     /locus_tag="IWJ07_002709"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP9334226.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          931 a          644 c          475 g         1024 t
ORIGIN      
        1 aagagacaag aaagaatcca tatggattct ttcttgtctc tttttccact actttttatt
       61 tttctgacac tacatattct tataactcat ttctcataac tgtgaaacaa cttataaaaa
      121 taacaaactt taaacaaaac aagcttaaaa tcaaagatga tccatcttaa aataactaaa
      181 acaaaaaaag tagtcgttgt taatcctcat taataatcgt tttctttttt tcaaacgctc
      241 catttttatg tggaacatta tttttttaag gaactttgtc aacatgtgga taactatttt
      301 ccacttgtgt cctggactag ctgttttgct gtaaaataca aaacttgctg cagcaaaaag
      361 atcgttgcaa gagaggctgt tccgcagatc gctggatcta gatttggcaa agtcaagact
      421 gcatcatgta aattggcgtg gttgtccaaa accaaatcag caaattcaaa taatcgcaaa
      481 ccactgctat gttgcgattt gatacttcgt gacaaatcaa atccagtaac aatgattaat
      541 ggatgaccat tttctttgat ccactgagct agttcgatga ttgccgggtt tcttccttca
      601 ttcgaaagaa gaagaaaaat gtcttctgat ttgacagaaa gctgatcaat aattgcctgt
      661 cctgcacctt ctagcgtttc atagacacct ccggctggat tttcaatcaa tatacttggt
      721 acaagatccg gggtttgatc ccaaaattca aatgctacac cacttagtgt gcggctagca
      781 tatatctgca atctaccacc ttgattaatt tgtttcgcga tccaagtgcc agcttgaagc
      841 atcagcggct cttctatcct taatttctct tccaattggt gataatagtc aaaataacat
      901 aaactcattt atctctcccg cctagctata cttgacatga atagaaacag ctgcctaatc
      961 taaggaagta cataaaaaaa cagtagttag acagtaacac atgtggtctt tgctgtttca
     1021 aacacaaaaa aggcattgaa cgaatacgcc caataccttg acatagcaaa taaaacaacg
     1081 tgcagaccat ggaaaatatc tacatagggg tagaattttc accctagatt aggtacaata
     1141 aattaggtcg atgcactgca ttgactatgg caaaatgatc ggtcagggtg tatgtgtgtg
     1201 actggtcatt ccctgattct tagcgccaac taagggtcag ggagcctttt tttattcagc
     1261 tgtttctatc tgaatttatt gtaagtaatc tcttatccaa gtgcaagcaa gaaaatacaa
     1321 aaaaacattg gtatgattta tatatatata aataacataa aaaaaggaat ggcttatccc
     1381 gatacaacaa gcattccttt aaaaaagatt cttttatata agtgaaaaaa gcttttttcc
     1441 accaattaaa taaatctctc atctcaatag tttatccatt attttatctt tttttgcgta
     1501 tacctactaa atattaccgt cttcttctcg ttcttctact ttgttcgaga gaatctgtac
     1561 tagaaaaagt agttgtatgt ctaccttttc tgcctatccg agactttctt ttcatatatg
     1621 ggatataaat gattagatac agtacagcaa aaaataaagc aaatacgcaa gcttcccaac
     1681 tacctaacgc caattcaacc atcaaggtag caaccccaac ggatgctgca taccagataa
     1741 aaatcagaaa cgtttctttt tttctgacca tgcgaaacat gataaataga ctaataagca
     1801 atagtggcaa cacaaatatc caattgcttc cttccatcag ccacttgagt atatcaagca
     1861 gcgactgtgc aacagcgact aattttccca tagtgaacac acaccttttt ttataattaa
     1921 aaaattcgcc tttttattct actatgaatc ttctggcttg gctttcattt ctcttatgta
     1981 atcattttct tttatacaac ttaaaaaaaa attaaaccat tagcagaata ttttctgact
     2041 ctattcacga aagtcttttt ctatctttcg ttttccacga ctcaactgat ctagagtcaa
     2101 aaaactaaaa cgacagttgc gcaaatgacg cacaagctgc cgctttaata acaaatagcc
     2161 tttcattctc agcaggccac ttatttccag ttaactttct gttcttagct ttttactcaa
     2221 ctggataata tatgcactta atgaagtccc aatttccgaa tgttgaagtc catattcaat
     2281 atttgtcttc atatatccta acttatcgcc tacgtcgtat ctttttcctt taaactctct
     2341 cgcatagacc gcctctgtct ggttaagagt ctgaagtgca tccgttagct gtatctcgtt
     2401 tcctgctcct ggttcttgag tttctaatat ctcaaatatt ttaggagtca gtagataacg
     2461 tccgataatg gctaaattac ttggtgcttt tcccatttca ggtttttcta caaacccttt
     2521 tactctgcaa cgaccctttt cgctatctgt tttaggatca ataattccgt atttagatgt
     2581 ttcattttct ggtacacgca tgacggcaac agcggaagct tgtgtttctt cataatccat
     2641 catcaattgt tgagtcaatg ggatatcatc tttcatcaaa tcatccccca gcatgacgac
     2701 aaacggttca tttcctataa atgcttttgc ttgaagcacg gcatccccca gtcctcttgg
     2761 atgagattga cggatgaagt gaagattgac ttgagtcgtt tcttcaacta aagcgagtaa
     2821 ttctgttttt tccttttccc gcaaattgtt ttccaattcg atatttgcat caaagtgatc
     2881 ttcaatgggt cttttttctt ttcccgttac gatcaagatg tcttcgattc ccgaatccaa
     2941 tgcttcttcc acaataaact ggatcgttgg tttatccacg attggaagca tttcttttgc
     3001 catggctttt gttgccggca aaaaacgtgt tcctagccca gcggcaggaa tgactgcttt
     3061 tttgaccttc actt
//
LOCUS       DACDPS010000159         3068 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.164, whole genome shotgun sequence.
ACCESSION   DACDPS010000159 DACDPS010000000
VERSION     DACDPS010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.164"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..503)
                     /locus_tag="IWJ07_002710"
     CDS             complement(<1..503)
                     /locus_tag="IWJ07_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP9334227.1"
                     /translation="MNSWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVPSINRLKHWVSVTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNRQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAIQEE
                     WERHELLS"
     gene            complement(514..1890)
                     /locus_tag="IWJ07_002711"
     CDS             complement(514..1890)
                     /locus_tag="IWJ07_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9334228.1"
                     /translation="MERKFGLRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     TVGTLFLVARFIDAVTDITMGRIVDGSKPSKTGKFKPWILRMSGPVALASFLMYQTSM
                     ASASMSIKVIYMYVTYILWGSIFYTSINIPYGSMASAITSVAEERTALSTFRSIGATL
                     AGMFIGVLTPIFIYTRDSSGAQIVRGGSTFTIVAGVFSVCAVICYVICYKLTVERVKS
                     DESQLKVSLLKSLKGLAKNRALLSIIAAAIFLLLAQLLTMSMNNYVFPDYYNNAQGIV
                     LMNFINPILMLVLVSPVSLIFSKKYGKKELASVGMFFSAIVYLLLFLIKPSNMYVFLV
                     LTSVGYMGLGIFNTVIWANITDVIDDQEVKSGQREDGTIYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLAKVQSGEVLQKLYNSSTLIPAVCFFVVGLILFFVYPLSKEKVLENY
                     NLLNQKHN"
     gene            complement(2019..2666)
                     /locus_tag="IWJ07_002712"
     CDS             complement(2019..2666)
                     /locus_tag="IWJ07_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP9334229.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINAITAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVEIIKLFPGSAFGPSVVKTYQAPLPYVNIMP
                     TGGVSLDNLEEWFEAGVVAVGVGGNLLAPVEKGDFEEVTRIASKYIDKFYEIKNY"
     gene            complement(2750..>3068)
                     /locus_tag="IWJ07_002713"
     CDS             complement(2750..>3068)
                     /locus_tag="IWJ07_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP9334230.1"
                     /translation="GMIQKIQLGCAWWFNDTAEGMRQQLTTMAQQSLLSNFTGMLTDS
                     RSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYLSQIVENICYRNAYNYFNFFEIE
                     SRK"
BASE COUNT         1016 a          624 c          379 g         1049 t
ORIGIN      
        1 gataataatt catgtctttc ccattcttct tgaatagctt ccttattagc tgagcttaca
       61 ataattatct tcccatattt tttcagagct tttatagtat ctctagcact taaaaagggt
      121 ttatctaatc ctttagcttg actaatctgt cgattgactt cttccgacca ttttaaagct
      181 tttaataaat cctctgactg agagtattct atttccctct tcaaagatgg attagacaat
      241 tcttttgtta cagataccca gtgctttaat cgattaatcg atggtacatc atttttttct
      301 aatgttttaa ctaaacctaa aaatctgttt attccacgag tctctgaata taaattaata
      361 ttattccaat cctgtaggaa tttttcccta tgatgaacat caaaaatctc tgcagcaatt
      421 ggcccgaaaa agagattgtg tttataagtc attgtatcca tgacacaacc atctgaatca
      481 atacaaaata tccaactgtt catgtttttt ctcctagtta tgtttttgat ttagcaaatt
      541 ataattttct aatacttttt ctttagataa tggatatacg aaaaataaaa tcaatcctac
      601 aacaaagaaa caaactgctg gaatcaacgt tgaagagtta tacagctttt gtaaaacttc
      661 tccactttgt acttttgcta acgaatcata ccctattata gaaagagtcc aacctccgac
      721 accgccagct aaagcttgtc caatttttcg ggcaaaagaa tatactgcat aaatagttcc
      781 atcttctctt tggccagatt tcacttcttg atcatcgatt acatcagtaa tattcgccca
      841 aatgactgta ttaaaaatac ctaaccccat atatccaaca gaagtaagaa ctaagaatac
      901 atacatatta ctaggcttta tcaaaaataa aagtaaatat acaatagcag agaagaacat
      961 ccctactgac gccaattctt ttttaccgta ttttttactg aaaattaaac ttacaggtga
     1021 aacaagtacc aacatcaaaa taggattgat aaagttcatt aaaactattc cctgtgcatt
     1081 attataataa tctggaaata cataattgtt catactcatt gttaaaagct gagcaagcaa
     1141 caaaaaaata gctgcagcta ttatagaaag caacgctcta tttttagcta atccttttaa
     1201 agatttcaac aatgaaactt ttaactgcga ttcatcactt ttaacccgtt ctacagttaa
     1261 tttataacat attacgtaac aaataactgc acacactgaa aatactcctg ccacaattgt
     1321 aaaagttgat ccaccacgta caatttgagc tccagaacta tctcttgtat aaataaaaat
     1381 tggtgttaac acgccaatga acatacctgc taaggtagct cctatactac gaaatgttga
     1441 taacgctgtc ctttcttctg caacagaagt gatagctgat gccattgaac cgtaaggaat
     1501 attaatagat gtataaaaaa tacttcccca tagtatatat gttacataca tatatataac
     1561 tttaatactc atagaagctg atgccattga tgtttgatac attaaaaaac ttgctaaagc
     1621 gactggacca ctcatccgta aaatccacgg tttaaatttg ccagttttag atggttttga
     1681 tccatccaca atacgcccca ttgttatgtc tgtaactgca tcaataaatc ttgctactaa
     1741 aaataatgtt cctacggtag ctccgcttat ccccaatact tttgtataaa aaatcattaa
     1801 aaaagaacta gcaaaaatga aagtgaaatc attcccaaaa tctccaaaca tatatcctat
     1861 tttgtctctt agaccaaatt ttctttccat ataatttgcg ctccttccta ttgttaagcg
     1921 ttttcattct gttgaactgc caacttacgt actatactct gaagtataaa gagttagtat
     1981 ttatttttta cattttatag tattatttat tttttctcct aataattctt tatctcataa
     2041 aacttatcta tatatttact agctattcga gttacttctt caaaatcacc tttttcaaca
     2101 ggagccaaca aattcccacc tacaccaact gcaacaacgc ctgcttcaaa ccactcttct
     2161 aagttatcta gacttactcc gccagtaggc attatattta catatggtaa tggagcttga
     2221 tacgttttta caacagatgg accaaaagca gaacctggaa atagtttaat gatttctact
     2281 ccagcggtaa gtgcctcctt tatctcagta attgtcatac aacctggtaa ataaggaatt
     2341 tgatataaat tacaaatctc tgctgtttgt cgatcaaaag atggacttac gatatattct
     2401 gctccagcca taattgcaat acgagcagta aacgcatcta aaactgtacc ggctccaatt
     2461 attacatctt tttgatcagc atatttttta gcaatttctt tgattatttc ttcagcattc
     2521 ggaactgtaa aagttagttc aatcccttta attccacccg cagtaattgc atttattgct
     2581 ttaatggcct catctttatt ttttccacgt acaaccgcaa tgactccagc cgcttctaac
     2641 ttacttaaaa tttctacttt tttcatatct atctccttct tttatggata catactctag
     2701 ctagttgtac taacttacaa taaataaatt taagctttta ttttttgtat tattttctac
     2761 tttcgatttc aaaaaaatta aaataattat acgcattacg ataacaaata ttctccacta
     2821 tctgactaag atacatttca tcatctggaa ttcgacctgc tttaacccac tcgcttagta
     2881 attgacataa tactcgacgg aaatactcat ggcgaggata gcttagaaaa cttcgagaat
     2941 cagtcaacat acctgtaaaa ttcgacaaca aactttgttg cgccatcgta gtcaactgtt
     3001 gacgcattcc ttcagctgta tcattgaacc accaagcaca tcccaattgg attttttgaa
     3061 tcataccc
//
LOCUS       DACDPS010000160         3021 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.165, whole genome shotgun sequence.
ACCESSION   DACDPS010000160 DACDPS010000000
VERSION     DACDPS010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.165"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(287..649)
                     /locus_tag="IWJ07_002714"
     CDS             complement(287..649)
                     /locus_tag="IWJ07_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334231.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(723..1103)
                     /locus_tag="IWJ07_002715"
     CDS             complement(723..1103)
                     /locus_tag="IWJ07_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAP9334232.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1185..2360
                     /locus_tag="IWJ07_002716"
     CDS             1185..2360
                     /locus_tag="IWJ07_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9334233.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2437..3021)
                     /locus_tag="IWJ07_002717"
     CDS             complement(2437..3021)
                     /locus_tag="IWJ07_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9334234.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
BASE COUNT          941 a          578 c          498 g         1004 t
ORIGIN      
        1 ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt
       61 tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt
      121 cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca
      181 ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa
      241 gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca
      301 ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat
      361 atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa
      421 cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga
      481 tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa
      541 gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac
      601 catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct
      661 cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact
      721 ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt
      781 aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt
      841 ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt
      901 ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat
      961 gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc
     1021 tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt
     1081 atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa
     1141 atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata
     1201 aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc
     1261 aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg
     1321 tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg
     1381 ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga
     1441 aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt
     1501 ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta
     1561 ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt
     1621 tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga
     1681 ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt
     1741 taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt
     1801 taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc
     1861 tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa
     1921 tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc
     1981 aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg
     2041 gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt
     2101 tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt
     2161 ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga
     2221 aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc
     2281 gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg
     2341 ctttagagac aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt
     2401 tcgtcctttt tcataattac tccgtaccat accaaactat tctgaaattt caattgtttc
     2461 aatcactaca tcatgtacag gtcggtcttg tggaccacgt tgtacatttg cgatttcatc
     2521 tactgtgtcc atcccttcta gtacatgtcc aaagactgta tgtcggaaat ctagccaagg
     2581 agttccgcca cctttatacg cttcaatgat ttctgatggg aatccagcac cttctagttg
     2641 tccaagcata ttagctggca catttgtgtt atttacaata aagaattggc ttccgtttgt
     2701 gtttggacct gaatttgcca tagataaagc tccgcgaagg ttgaacagtt cacgactgaa
     2761 ttcatcttca aaactttcgc cataaatact ttcgccaccc attcctgttc cagttggatc
     2821 gccgccttgg atcatgaaat caggaataac gcggtgaaaa atcacgccat cataataacc
     2881 ttttttggaa agttcaatga aattttgaac cgtcttcggt gctagttcag ggaataattg
     2941 aacagtaatg tcaccacggt ttgttttgat aacagctttt ggtccttttt cactttttaa
     3001 atctagttgt gggaatgcca t
//
LOCUS       DACDPS010000161         2994 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.166, whole genome shotgun sequence.
ACCESSION   DACDPS010000161 DACDPS010000000
VERSION     DACDPS010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.166"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..947)
                     /gene="sgrA"
                     /locus_tag="IWJ07_002718"
     CDS             complement(<1..947)
                     /gene="sgrA"
                     /locus_tag="IWJ07_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAP9334235.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASY"
     gene            complement(1143..1982)
                     /locus_tag="IWJ07_002719"
     CDS             complement(1143..1982)
                     /locus_tag="IWJ07_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP9334236.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            2167..>2994
                     /locus_tag="IWJ07_002720"
     CDS             2167..>2994
                     /locus_tag="IWJ07_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAP9334237.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     "
BASE COUNT         1005 a          628 c          451 g          910 t
ORIGIN      
        1 aaatagcttg ctcctacaat caaaatgagt aacacaccca tccatgtaat tagcttgttc
       61 aaggttctac taccagtttc tggtaaatcg ctttttctat tatctgtttc tttcgctttt
      121 tcttgatgat caattccacg agaaacaaaa tcaggctgtt ttagcacact ttttaggttg
      181 ctccctgcat ttctcttttg ttccttagtt ggtatgatag gtaccaaatc aagttcatta
      241 tttacttttc ctgtgctgct ttcgctgctc gtgcttcctg tgctactctc actactcatg
      301 cttcctgtgc tactttcact actcgtactc tccgtactgc tttcgctgct cgtgcttcct
      361 gtgctacttt cactactcgt actctccgta ctgctttcgc tgctcgtgtt ttctgtgcta
      421 ctttcactgc tcgtactctc tgtactgctt tcaccgctcg tgttttctgt actgctttca
      481 ctacttgtgc tatccgtact gcttggcggt ccaacaactg tccaactgct tggtttcaaa
      541 ttctccgtga tcgtcatctg tccatcataa aaaacaatca aatcaacttt atctttactc
      601 ttgatctcat caatcgatag ttctgcagaa atcattattg tatactcaat accagcggtc
      661 cattgtacgt cttttaaata aacttcccca ataacttttt ctgaacttcc attaccatcc
      721 caaccatatg ttagaacagc atgtacatca ctagcattca cgcttttgtt tggtttgact
      781 ggaatagtaa tgacttgttg tgtcatgtca taaacaatcc gatcagtttt cacagtaaat
      841 gaaagattct catttgcccg ttcatttgca gaagtactga ttccaatcat tgctgcaatt
      901 atactcagcc caagaataac aaataatacg gtcgctgttt tcttcatttc tatcactctc
      961 catataatta ccttctattt gataaaggaa ttatataaaa tgaaaatgat gagaacacga
     1021 cagtcagttg tgaaaatcta acaaaagatg tcgtgtattc gcttgaattt gtttactttt
     1081 tcgtttaatc aatgacagac ttaaaaaagc aagaccgata tcagccttgc tttcttcatc
     1141 ttttattgat aaacagcagt gtatttcttc atccgctttg ttttatatgc atagagtaac
     1201 aaaccaacaa tcaagactaa caagaataat gtagttgata tagatgcttc agccccaaag
     1261 tttcccccag taattattgt attttgcgat tggatcagag tggagaaaaa accttgttct
     1321 tgcgttccgc tgactggtat acccaaaata gctccttgtg tccaattcca agtaatatga
     1381 gcaccaatag ccgtaaataa acttcctgta ctgtagataa gtgcgctgaa aaagcaacct
     1441 gctaaaaaga tattgaaaac tgccagaaat gtcatgttag gattcaatcc gtgcaaaaga
     1501 gcaaataatg cagcagatcc aatcattgct attaacggac caaatctaag catctgatgc
     1561 tgaaaaaagc cacggaataa aagctcttct gatgccccca ctgcaataaa taaaataaaa
     1621 ctcgttatca gcggcacaat gtttgcgttt cctgaatcta accgatacgc ccccgtcaaa
     1681 tataatggac tagtaataag cagaaacact ccaattccaa caacgagacc tcccgctaaa
     1741 tacccccagt cttttcctcg aacagaaaca ccataatctt ctattctttt tccttcgacc
     1801 ttactagcaa gaaataaagc tagtaaaaaa acagctaaat gtccgaccgt accggcaatt
     1861 gctccaatcc attcactatt tccaataaac atatccgcaa gtgcattatt gacaagaatt
     1921 cctaaaacaa aaccaactaa gactattaaa acagatccaa tcaaccgaat aatcctgatc
     1981 atctttcatc cctcctttat attaagttta acaacccttt tctttcttgt ctcgtgaaaa
     2041 ctatgtaaat gttctccatc atagagagaa tagattttta cgaactaaga atatctacag
     2101 caaccttacg aaaatgaaag actgtctccc taattcttct gttagactgt ttttaataag
     2161 gaaaggatgg gaaatttttt gaaaaaacaa ctactaatta tgtgcctttc agcatgtgca
     2221 attttactat tctcaggttg tagcaagtcc aatgaaacga ttgcaacgtt taaaggtgga
     2281 acacttacag ttcaggattt ttatgataac ccaaaagtta aatcaaataa tcaaacacta
     2341 ttaaaaaaaa tgattgtctt caaagcattt gatgatgtat acggaaaaga gatttcttca
     2401 aaggaagtaa caaaagaata taatgaacaa atcaaaaaac tcggtccaaa ctacaaggaa
     2461 caattaaaag cagttggcca aacagaagag acatataaga aactctttaa acagatgctg
     2521 gctttccaat acggtttgaa agccaatgta aaattaacag ataaagatct agacactgct
     2581 tggaaagaat tctatccaga agttagtaca cagatcattc tattttcgac taaagaagaa
     2641 gcagataaag caaaaaaaga agcaaatgaa ggggaaaatt tctccaagct tgttcaagct
     2701 tatggaaaaa ataaaacatt aaaggaaact gatggaaaaa tgaattttga ttcaaccaat
     2761 ccagagattc ctactgaagt aaaaaaagca gcatttaaac taaaaaacgg ggaagtttct
     2821 gatatcattc ctgtaacaga tccaacgact tatcagcaaa gctattatct agtaaaaatg
     2881 gtaaaaaaac aggataaagg ttctaataaa gacaaataca aatctgagct agaaaaaatt
     2941 gctaccgaag caaaaactct tgatacggaa ttcatggata aaacaatccg taaa
//
LOCUS       DACDPS010000162         2848 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.167, whole genome shotgun sequence.
ACCESSION   DACDPS010000162 DACDPS010000000
VERSION     DACDPS010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.167"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            11..667
                     /locus_tag="IWJ07_002721"
     CDS             11..667
                     /locus_tag="IWJ07_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9334238.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            667..2043
                     /locus_tag="IWJ07_002722"
     CDS             667..2043
                     /locus_tag="IWJ07_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9334239.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2329..>2848)
                     /locus_tag="IWJ07_002723"
     CDS             complement(2329..>2848)
                     /locus_tag="IWJ07_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HAP9334240.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSLEQLLSTVCDVGDISFSIRNQLRFKRSLPVSGNINVYFTKARFKRLIAITVSSIV
                     MFLWLFRMGVIP"
BASE COUNT         1079 a          383 c          508 g          878 t
ORIGIN      
        1 ggagaaaata atggatatat tagaaataaa gaatgtagca tatagttatg caaattctaa
       61 agaaaaagtt ttgtcaggag taaatcaaaa atttgaactt gggaagtttt atgcgatagt
      121 aggaaagtca ggaacaggaa aatctacact tctttcctta cttgctggac ttgataaacc
      181 tcaaacagga aaaatattgt ttaagaatga agatatacaa aataaaggat atagtaatca
      241 tagaaaaaat aatatatctt tagtatttca aaactataat ttaatagatt atttatcacc
      301 aattgaaaat attagattag taaataaatc agcagatgaa agtatcttgt ttgaactagg
      361 tttagataaa aaacaaataa aaagaaatgt tatgaaatta tctggtggac agcaacaaag
      421 agtagctatt gctagagcat tggtatcaga tgctccaata atactagctg atgagcctac
      481 tggtaatcta gacagtgtta ctgctggaga aataattaat atattaaaga cattagctaa
      541 agacagaaat aaatgtgtaa tagttgtaac acatagtaag gaagtagcag attctgcgga
      601 tatcatttta gaactaagtg gtaaaaagtt gaaaaaagta aataaaatga atttggaggt
      661 tgaataatga taaaaaatgc atttgcttat gtaactagaa aaagtttaaa atcattaatt
      721 atcatattag ttattttatc tatgtcaact ttaagtctca ttagtttatc tattaaagat
      781 gctacggaca gagcttcaaa agaaacattt gctaatataa caaatagttt ttctatggag
      841 ataaatagac aagtaaatcc aggaacacct agaggtgggg gaaatgtaaa aggtgaagat
      901 attaaaaaga tatctcaaac agatagtata gactcctatg taaaaagaat aaacagtgtt
      961 gcagatttag ttgattatga tattattgaa actcaagaga ctcttgcgaa tcaatcacct
     1021 gaaagagcaa agaattttaa aagaacagtt atgttaactg gggttaacga ctcaacaaaa
     1081 gaaacaaaat ttgtatcaga agcatataaa ttagtagaag gaaagcattt agaaaatgaa
     1141 gataaaaata aaatcttaat gcataaagat ttagctgaaa aaaataacct taaagttgga
     1201 gataagataa aaataaaatc taatttattt gatgctgata atgagaaagg tgcagatgaa
     1261 acagtagaag tagaaattaa aggtctattc gatgggcata atagcggtgt agtaagtgca
     1321 gcacaagaac tatacgaaaa tacattaatc actgatattc atacagctgc taaagtttat
     1381 ggtaataccg aagatacagc tgtataccaa gatgcaacat tctttgtaaa aggtgataag
     1441 aatcttgata gtgtaataaa agatcttggg aaattagata taaattggag agaatataat
     1501 ttgattaaaa gttcatcaaa ttaccctgca ttacaacaat ctatatcagg tatttactca
     1561 atttcaaata aattattttt tggctcatta atatttgcag gagttgtagt ttcattatta
     1621 ttattcttat ggatgaatgc tagaaagaaa gaaatagcag tattactatc attaggtata
     1681 tcaaaattag aaatttttgg tcaattccta attgagatgg tatttatatc aatccccgca
     1741 ttcgttggtt cctatttctt agctcaatat acagctgaca agcttgggaa taatatattg
     1801 aataaggtta ctggtgatat agctaaacaa atagctaaac aatctgcatc tagccaatta
     1861 ggtggtgggg cagaagctga aggatttaac aagacattat caagtttaga tatcaatgta
     1921 ttacctaaat tcatgattta tgtagttata tttatgagtt tagtacttct tgtatcattg
     1981 attatttctt cattcaatat attaagaagg aatccaaaag aattattaat tgacaataat
     2041 taaaattttc ggtgcttttt agcaccaaaa atgagagtgt aatataaacc gtgtaagtaa
     2101 ccatcaatcc taggcgggtg gttgcttcac ggtttttatt tttctaaaat aagatcaaga
     2161 ggtgattaca cgatggctag aaagaaaaga aatcctgatg ctgagaagtt agctgaatcc
     2221 attttgaata cttatcaacc cgaatctgta gaggatatgc aagatgtttt aaaagatgtg
     2281 tttggtccct tatttgaaaa aatgcttcaa ggagaattga ataaccattt agggtatgac
     2341 gcccattcta aagagccaaa gaaacatgac aatcgacgaa acggttatgg caataagacg
     2401 cttaaaacga gctttggtaa agtaaacatt gatgttccca gagaccggga ggcttctttt
     2461 gaacctgagt tgattcctaa tagaaaacga gatgtctccg acatcgcaga ctgtagacaa
     2521 aagctgttcc aaactcgtaa ctcgagcttg gaacagcttt ttatctactt cgtttcgaat
     2581 ttttgaacaa aaaaataaag gctccttgcc tttccatagc caggtggcta atttctttaa
     2641 attcattgca gcaaacacta gcatcgtatg cgtcgcgact ttttccaagc ctcgatactt
     2701 ggtccaacgc atcccatgct tttcttttgc atccgcaaat attcgttcaa tcgtttgttt
     2761 ccgttttttg taaattgatt tattcagatc tgttaggcga agatgttcca cttcatccat
     2821 cagatctccc caaacatgtc tcgtgatg
//
LOCUS       DACDPS010000163         2802 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.168, whole genome shotgun sequence.
ACCESSION   DACDPS010000163 DACDPS010000000
VERSION     DACDPS010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.168"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            5..1087
                     /locus_tag="IWJ07_002724"
     CDS             5..1087
                     /locus_tag="IWJ07_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9334241.1"
                     /translation="MNDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1305..2447)
                     /locus_tag="IWJ07_002725"
     CDS             complement(1305..2447)
                     /locus_tag="IWJ07_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9334242.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYMLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTIEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2452..2637)
                     /locus_tag="IWJ07_002726"
     CDS             complement(2452..2637)
                     /locus_tag="IWJ07_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP9334243.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
BASE COUNT          930 a          511 c          441 g          920 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaaccac cggtggcaaa ccggtggcaa atccatgtaa
     1141 acatggttta ttttttacac tgttttcaaa caaacccttg ctatttcttc ggttcgtcct
     1201 cgtccatttt cgataccgac agctagctta taacccaagg tatatcaaca taaaaaaata
     1261 aaaataggct gtgacagcag cgtgacagca aaaaagatgg aaagttaaat atcaaacctc
     1321 gtaatttcat ttgcgagtgc ttgatttgtt tctggatata agtgtccata tttgtccaaa
     1381 gtagttttaa tagaggcatg acctagacgc ttagatattg catagatatt tttattatgt
     1441 tcaattaaaa aggcaacatg cgagtgtctt aatccatgca tatgaattgg ttttactcct
     1501 gcaatttcag ctagtttttt tattctgctt aaaatagttc ttggagatgg cggtaatcca
     1561 tcaaaagaaa aaaccaattc tatatttttc ccaattgatt gttgagtttt tttccatgtt
     1621 tttaaatctt caatggtttg tctgtctaac gaaattatcc gaatacttga tgtatttttt
     1681 gtttcagcaa attcataatc ttgtcttgta cggatgtaaa ttgacttatt tacgctacat
     1741 ctgccattta ttaagtcaaa gtctttccat tgtaacgcta agagctcctc actccttaca
     1801 cctgtaacaa aaataaaacg catcaaacgt ttataaaatt cttcataaaa atccccgtga
     1861 taaacactat tatatacttt cttaaattca tcaactgtcc aaaattctac ttgaggacgc
     1921 tgagttttta ttttacctat agtatctaca ggattttctt caataatttc aagtacaatt
     1981 gcccttttaa aaactactct caacttattg aatatctgtt taatataatt tggtgataat
     2041 ggttgctcaa tgcccttttt tacaacatgg attttcgata aaaactgttg aaattcctgt
     2101 atatgaattg gtcgaatctt tacaacctgc atttctccaa aaaaatccaa tgcacgttgt
     2161 aaaatatgat ctgttttcat atatgttttt tctacagttc ccaacatata ccaaggaaaa
     2221 aaatactctc gataaaaatc atgaaacgtt attccttgca gataaattcc ttgtgaatca
     2281 gaaaaacttt gttttaactc ttcgagtccc cttctagctt ctccttgagt tttgaagcct
     2341 cttctggtca cttgaattct ttttccaaag cgatcttgtc ctaaagttgc agaaaaaaac
     2401 caagtgcctc tttttctgtc tttataaaca ttcgcaattt tcctcatttt actattcctc
     2461 ccaagaaata tggagaactt cttctaaaat tgaaatagga attcttttta tttttgaatt
     2521 acgataaaaa aagtatcctt tctcaactaa aagttcttta cattccttga agattttatt
     2581 agcatagtac tgagaaaaac ctagattaat caaatctttt cgagtagctg ttttcattat
     2641 agcaccttcc ttactttttt tcatttattt ctatcagctg cttttgtcaa aagcacatag
     2701 gaatttcacc tctccccaag tttacggcga gccttacgga agtatcatta tcccaacttt
     2761 aaatcatcgc tttttattga ctataaaaat tattttagaa ta
//
LOCUS       DACDPS010000164         2800 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.169, whole genome shotgun sequence.
ACCESSION   DACDPS010000164 DACDPS010000000
VERSION     DACDPS010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.169"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            142..1209
                     /gene="rlmN"
                     /locus_tag="IWJ07_002727"
     CDS             142..1209
                     /gene="rlmN"
                     /locus_tag="IWJ07_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP9334244.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(1402..2505)
                     /locus_tag="IWJ07_002728"
     CDS             complement(1402..2505)
                     /locus_tag="IWJ07_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9334245.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
BASE COUNT          891 a          494 c          556 g          859 t
ORIGIN      
        1 ttttctaata cggtatgatg tcttgtcttt ttagtttttg tgagattaaa gttctattcg
       61 atttatttgt gaagtattgc attaaggaaa tgaatcatgt atagtatgta gcgaaacttt
      121 gagaaaaaga gggaaaatac tttggaaaaa cagtccattt atggcttgac gaatgaagaa
      181 ttaatcaatt ggtttataga aaacggcgag aaaaaattcc gagctgccca agtttgggaa
      241 tggttgtatc aaaaacgtgt aagtaatttc acagaaatga cgaatctatc gaaacaattg
      301 atcgaaaaat tatcggagca ttttatcatc aatcctctaa aacagatggt cgtccaagaa
      361 gcttcggatg gtacagtaaa atatttgttt gaactccctg ataaaaacat gattgagact
      421 gtactgatga gacaagaata tggcttgtca gtttgtgtga cgacacaagt aggatgtaat
      481 atcggctgta cgttttgtgc aagcgggctg ctcaaaaaga accgtgatct gactgctgga
      541 gaaattgttg cacagatcat gatggtccaa cattattttg atgagcgcaa tcttggcgaa
      601 cgcgtttcac atgtcgttgt aatggggatc ggtgagccat ttgataatta cgacaatgta
      661 atggactttt tgcatatcat caatgatgcc aaaggattgg caattggtgc tcggcacatt
      721 acagtttcca ctagtggatt agcacacaaa atcaaggaat tcgctaataa cggcttgcaa
      781 gtcaatctgg ctatttcctt gcacgcgccg aataatgagg tgcgaacatc gatcatgcgg
      841 atcaaccgaa gtttcccaat tgagaaattg atggaagctg tggatgagta tttagagaaa
      901 acaaaccgtc gaatcacgtt tgaatatatc atgttgaatc aagtaaatga tagaccagag
      961 catgcgcaac aattagcaga tctattaaaa gataagaaaa aattagcata tgtcaacttg
     1021 attccatata atccagtcag tgaacatgat caatattctc gcagtccaaa agctgatgta
     1081 ttgagatttt atgatgtatt gaagaaaaac ggcgtaaact gtgtgatacg taaagaacat
     1141 ggtacagata ttgatgcagc ttgcggacaa ttgcgaagca agcaaatgaa aaaaacagtt
     1201 gccaaataat cgtatcctac tcgttttttc aattaccata aaaggagcag ttttcttaat
     1261 taaggaactg ttcaacttaa ataagagaaa agattctata gcgaaagcta taaagctttt
     1321 tttgacaaga aaaaccctca tcaagcaaag aaatgtcctc aatttgatga cagcctgctt
     1381 gatgggggtt ttagcaatta tttaactgat tttcttatat ccttgctggc tttcttgtgt
     1441 agcatgataa gtttctacgt caagaatggc ctgtttaaat ttttctcggg tgatttcata
     1501 aggcacatgt ttaatatcat ttgttgccat ggctttatct aagattgctt ctaaatcggt
     1561 cgtgacattg agattcatgt cggttaacga tactggaagt tggttctttt tcataaacac
     1621 atagatttta tcaaacaaat cgaactgctg atccatcatc aataaggtca gtacaccata
     1681 actgacagat gctccgtgaa ggtatccctc cattttttct agaacagtcg taccgtaata
     1741 gaatgagtgt gccatagcag aattatagtt attgttaact aaaacagaaa ccaatccggt
     1801 agtaacaatg atatccgaaa cgacttgttc aattgcatat gagggacgat cgtcggcact
     1861 atctttcaat gcttgcgtac cataagtcaa gagacgatca atgcacattt ttccgatttg
     1921 gattcctaat ctatccgtgt agcctagatc atcgccacga gcagaaaaag tagattcgac
     1981 ttctttggat aaagcatcgc caatacctgc ccataagtat ttatcaggtg cttcggcaat
     2041 aattgctgta tcaatgaagg tgtgtacagg tggttttgaa ggaaagaaga gtccgtccat
     2101 tacgccttct tcgttataga tgacacatac cgcagtgacg ggcgaacagt tcgaaccaat
     2161 agtgggaaag gtgaagactg gtttagctag tttttcacca atgactttta cagtatcgca
     2221 agcacggcca ccaccgactg caaacaggac gtctgctttt tggacacttt catgctggat
     2281 caactgttca atatttccat aagttgaatt acccccgtac cagagactgg cgacaacctc
     2341 tatttcctgt gtcaaagcta cttttatttt gttttctgcc tttgaaagtg cagtcttccc
     2401 tccaatcagc actacttttg tcccatattc tttgacgatt ccatttgctt tttcatacgc
     2461 atctgttcca atggtataat ttggtaagaa aacactttga ctcatattca tttccttctt
     2521 tcttcgttga aacgggttaa aattttaaag agtgatcgat ctgactgcgg ctgtagatgg
     2581 agtagaggtg aacctaagac aggataagtg atgaataagg tggcatgaac accttgaaat
     2641 catcaataag tatcctaaaa acgaacaagt acaaaaacat tgaaaacgac aataatgaat
     2701 ttttgttcct cttagtattt ctaccgattc cctttgcttt tttgtaatta tctaaaaatt
     2761 ctaaaaatta agattattat atgaaaacag gttttatttg
//
LOCUS       DACDPS010000165         2784 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.170, whole genome shotgun sequence.
ACCESSION   DACDPS010000165 DACDPS010000000
VERSION     DACDPS010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.170"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1350
                     /locus_tag="IWJ07_002729"
     CDS             <1..1350
                     /locus_tag="IWJ07_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9334246.1"
                     /translation="TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDT
                     VKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPALPEGTTVTDVTPGGT
                     IDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTDLNKEPDA
                     SEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSD
                     ADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVT
                     YPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHG
                     TADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTD
                     NQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            1484..>2784
                     /locus_tag="IWJ07_002730"
     CDS             1484..>2784
                     /locus_tag="IWJ07_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9334247.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPY"
BASE COUNT         1091 a          383 c          542 g          768 t
ORIGIN      
        1 actaacttgc cagcgttacc agaaggaaca acagtaacag atgttactcc cggcggtaca
       61 attgatacca acacaccagg taattacgaa ggtgtcattg aagtaacgta tccagacggt
      121 acgaaagata cggtgaaagt tccagtagaa gtgacagaca atcgttctga cgccgataaa
      181 tatgaaccaa cagtagaagg cgaaaaagta gaaattggag gcacagtaga tttaacagat
      241 aatgttacta acttgccagc gttaccagaa ggaacaacag taacagatgt tactcccggc
      301 ggtacaattg ataccaacac accaggcaat tatgaaggtg tcattgaagt aacgtatcca
      361 gacggtacga aagatacggt gaaagttcca gtagaagtga cagacaatcg ttctaatgca
      421 gataaataca atcctgaagg acaaaaagtg actacagact taaataaaga gccagatgca
      481 tctgagggaa ttaagaacaa agaagattta ccaaaaggaa ctacttatgc ttggaaagag
      541 aaagtagatg ttagcacagc tggaaataaa aaaggtacta ttgtagtgac atatccagat
      601 ggatcatctg atgaagttga agtagacgtt acagtgacag atactcgttc cgatgcagat
      661 aaatataatc ctgaaggaca aaaagtgact acagacttaa ataaagagcc agatgcatct
      721 gagggaatta agaacaaaga agatctacca aaaggaacta cttatacttg gaaagaaaaa
      781 gtagatgtta gtacagctgg aaacaaaaaa ggtactgtag tagtaacata tccagatgga
      841 tctaaagaag aagtagaagt tactatttct gtagtagata aaaaagcacc gaataaacct
      901 caagttgatc ctattacaga gggtgaccaa attgttactg gtaaaactga accaaatgca
      961 gaggtgacag ttacattacc agacggaagt caacaccatg ggacagctga taaaaatggt
     1021 aattttacag ttaaagttcc taaattagag gcagggacaa aagtgatagt aactgcaact
     1081 gatgaatctg gtaatactag tgaacctact aatgtagttg tttcttccaa caaaaaagac
     1141 agtggaaaaa acggtagtaa aggtagtaag actgacaatc aaggtagcaa cagcaatcaa
     1201 gacaaaaata gaggcaaaag ccagtcctca aaagtttttc ctaagacggg agagagtgac
     1261 agtaatatct ttactattag cggtggttta atactgttag gaacgttagg attattaggg
     1321 tataaaaatc gaaaaaaaga gaatgaatag aaaatcaatt tcttaagtct tctctgagaa
     1381 gaggaagaat ctttttcttc ctctttttgc ttttttatga tgggaaataa gtgttagtta
     1441 aagatgaata tactttttat agaaaggaaa agaatgtaat taagtggaaa aaatagtaat
     1501 tattggttca aaccattcag ggctagccgc agctcgattt ctaaacttaa gcgattcaaa
     1561 ttatgaagtt attctaatcg ataaaaaatc agaaattagt tacttggaat gtgggacatc
     1621 gttatttata aaaggagaag taagtaattt agaagatctt ttttatcaaa atagaatgac
     1681 actaaattct gagataaaaa agttatttct agagacagaa gttgacaaaa ttgatattca
     1741 aaataaaaag attagttgtg taaaaaaaga tggtacaaga ttctctcaag aatatgacaa
     1801 agttatatta gctacaggtt caaagcaaat tatgctagat ataccgggga ataatttagc
     1861 taatattttt tcgataaaga attttccaaa tgcaaaagag atttttaaag ctttggatga
     1921 tagtactatt aaaaaagtag gaattattgg agcaggatat attggagttg aaattgctga
     1981 agccataaga aaaagaggga aagaagtata tcttttcgat gttgcagata gagtgttgtc
     2041 gacttattac gaccgtagtt tttcagataa agttgaagaa atactatcaa aaaatggaat
     2101 acatctttgt ctatcagaaa gtcctaaaaa atatttagga agaaaaaaga ttgaaaaaat
     2161 agtcactaca agtggtaccc aatactctat ggatatggtc gtacaagcta taggatttct
     2221 atctaatagt ccaattgggg aaaaggaact cttgagagat gttagtggag catatctaac
     2281 taatgaatac cagcaaacaa atataaaaga tatatatgct attggggatt gtgctacaac
     2341 tttttttacg tccattaata gaagtagtgt tgatttttct atttctaatg ctctaagaac
     2401 agcttatatt gcaactcaac atattaacaa tcaagatttt acaccttctg gttcccaatt
     2461 tacaaatggc ttttctatat ttaattacaa tttttacgca gcaggtttga atttaaaaac
     2521 agcacgatta ttaagtgagt ctatagatta tatagaaata gaagatttca ttagacctgc
     2581 ttttttgaga aaaaatagtc gtgtaaaatt aagaattagc tttgaaaaaa atacaaagag
     2641 aatagtaggt gtacaacttt gtagtcaaga agatttatct ggactattat caatgttttc
     2701 tcttgctata gaagaacaaa ttacattaga caaattgaaa atgttagatt atttatttca
     2761 tcctagtttt agccaaccct ataa
//
LOCUS       DACDPS010000166         2780 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.171, whole genome shotgun sequence.
ACCESSION   DACDPS010000166 DACDPS010000000
VERSION     DACDPS010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.171"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..197
                     /locus_tag="IWJ07_002731"
     CDS             <1..197
                     /locus_tag="IWJ07_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334248.1"
                     /translation="WFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLIIAIIAVGLV
                     FNAAGVKDILLELFNRIIGA"
     gene            314..811
                     /locus_tag="IWJ07_002732"
     CDS             314..811
                     /locus_tag="IWJ07_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP9334249.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSIGELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMYDVARYYIEETGALGEVPASLQNYIDYQAYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            786..1292
                     /locus_tag="IWJ07_002733"
     CDS             786..1292
                     /locus_tag="IWJ07_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9334250.1"
                     /translation="MGFLKSSIKSVGTLLLADFLFYGVAQSATPIFYERIDYMKKIRS
                     YTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAVMILGNLPPLSMIEGAFLKYF
                     GIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRPKLTYAGKKVTLGRNQPQEAITAVR
                     SEFYGISN"
     gene            1276..>2780
                     /locus_tag="IWJ07_002734"
     CDS             1276..>2780
                     /locus_tag="IWJ07_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HAP9334251.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSIRSAQERSKNEVTGKLKAVAYDKIDQQTDALISM
                     IGENQVNYRFFIGFKLLLNDQEFSMKSLTVEAKNALSDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYHLSKKKLDN
                     ETLIKYYDLIKPTRCLVEEKQRYLKIQQEDETVYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKA"
BASE COUNT          839 a          515 c          600 g          826 t
ORIGIN      
        1 actggtttct atcaaacgct caatcactgg tgcttttagc aatcgttgtg attggcttgt
       61 atcttggctt caagcgtgag tttagcaaac tgattggctt tttaattatt gcgattattg
      121 cggttggctt agtcttcaac gctgctggag taaaagacat tttactagag ctattcaatc
      181 gcattattgg tgcttaaata aaaccgttct tttgtggaat ataagtggtt ttcttatgtt
      241 ccgcaaagga atggtacacc aaacgaagtg cggtagggat ttttgaatct ctacaaagaa
      301 aggacgtgaa tatatggacg atatgcaagt ctatattgcg aatttaggca aatacaatga
      361 gggcgaattg gtcggtgcgt ggtttacctt tcccattgac tttgaggaag tcaaagagaa
      421 aatcggcttg aatgatgaat atgaggaata cgccattcat gactacgagt taccctttac
      481 ggttgacgaa tacacttcca ttggcgaact caatcgacta tgggaaatgg tatcggaatt
      541 acccgaagaa ttacaatcgg agctatctgc tctgctcact catttttcaa gcattgaaga
      601 actaagcgaa catcaagagg atattatcat tcattccgat tgtgatgata tgtatgacgt
      661 ggcacgctac tacattgaag aaacgggtgc tttaggcgaa gtaccagcta gtcttcaaaa
      721 ctatattgat tatcaagcct atggtcggga tttagacctt tcaggaacgt ttatctcaac
      781 caatcatggg atttttgaaa tcgtctatta aatctgtcgg tacattacta ctggcagatt
      841 ttctatttta cggggtggct caatcagcta cccctatttt ttatgaaagg attgattaca
      901 tgaagaaaat acgaagctat accagtatct ggtctgtgga aaaggtactg tattctatca
      961 atgattttag acttccgttt cccataacct ttacgcaaat gacatggttt gtcgtgtcac
     1021 tctttgcagt gatgatactt ggcaacttgc cccctctttc catgatagag ggagcatttc
     1081 tcaaatactt tgggattcct gtggctttca catggtttat gtctacaaaa acttttgatg
     1141 gtaaaaagcc ttatggattt ttgaagtctg tcattgctta tgcactgcga ccaaagctga
     1201 cctatgcagg aaaaaaagta acgcttggca gaaaccagcc acaagaagcc attacagcag
     1261 ttaggagtga attttatggc atatccaatt aaatacattg aaaacaatct cgtctggaat
     1321 aaagacgggg aatgttatgc ttactatgag cttgttcctt acaattactc atttctaagt
     1381 ccagaacaga aaatacaagt gcatgattct ttcagacagc ttatcgcaca aaatcgtgat
     1441 ggcaaaattc atgctttaca aatcagtaca gaatccagca tacgttctgc acaagagcgt
     1501 tccaaaaatg aagtcactgg caagctcaaa gcggttgcct atgacaaaat cgaccaacag
     1561 acagacgctt taatatccat gattggcgaa aatcaagtga actaccgttt ctttatcggc
     1621 tttaagttgc ttctcaacga tcaggagttt tctatgaaaa gtcttaccgt tgaagcaaaa
     1681 aatgctttgt ctgattttgt ctatgatgtg aaccataagc tgatgggcga ttttgttagt
     1741 atgagtaatg atgaaatcct gcgttttcag aagatggaaa agctcttaga aaataaaatc
     1801 tctcgtcgtt tcaaaatccg caggttagat aaggacgact tcggctatct gattgaacac
     1861 ctttacggac agacaggcac tgcctatgaa gagtatgagt accatctatc aaagaaaaag
     1921 ctggataatg aaacgctgat taaatactat gacttgatta agcctactcg ctgtttggtg
     1981 gaagaaaaac agcgatattt gaaaatccag caggaagatg aaaccgtcta tgtagcttac
     2041 tttaccatta acagcattgt cggagaactg gacttcccgt cctctgaaat cttctactac
     2101 cagcaacagc aatttacatt cccgattgat acgtcaatga atgtggaaat tgtagcgaat
     2161 cgtaaagccc tatctactgt ccgcaataaa aagaaagaac tgaaagactt ggataaccac
     2221 gcttggcaaa gtgataatga aaccagctcc aatgtggcgg aagctctgga aagtgtgaat
     2281 gagctggaaa ccaatttaga ccaaagcaag gaatctatgt acaagctgtc ttatgtggta
     2341 agggtatcag caaatgatct tgacgaactc aaacgtcgtt gtaatgaagt gaaagatttt
     2401 tatgacgatt taagcgtaaa actggtacga ccatttgggg atatgctcgg cttacatgaa
     2461 gaatttttac ctgccagcaa gcgttatatg aatgattata ttcaatacgt gacctctgat
     2521 ttcctcgctg gtttaggttt tggtgctact caaatgctgg gggaaaatga ggggatttat
     2581 gttggctaca gcttagatac tggacgcaat gtctatctga aacctgctct tgccagtcaa
     2641 ggggttaagg gttcagtaac caatgcgtta gcgtcggctt ttgttggttc gctgggtggt
     2701 ggtaaatcct ttgcgaataa ccttatcgtc tattatgcgg tgctttatgg ggcacaagca
     2761 gtgattgtag acccaaaagc
//
LOCUS       DACDPS010000167         2752 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.172, whole genome shotgun sequence.
ACCESSION   DACDPS010000167 DACDPS010000000
VERSION     DACDPS010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.172"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            440..958
                     /locus_tag="IWJ07_002735"
     CDS             440..958
                     /locus_tag="IWJ07_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAP9334252.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT          943 a          402 c          494 g          913 t
ORIGIN      
        1 tttggacaat tgaaaataca aaaaaaaaga aaggccctgt gagactcgga aggctaccaa
       61 ccttcgtcga cgtccacagg tggcattaag atcactccct taatgcgttt acctttcagt
      121 gttacggtat tacccgtaac ggatttagta taacagatat tcaaaagtga taccactttt
      181 tttggctgtg ttaaggttaa aaatacgtga tattacgatg aatctatgcc taagcataat
      241 gttataaaaa tgtccgtgtg gctctttgcc gtatgacgaa cgctgaaatt aggagacgtt
      301 gggatatttt ataaatcatt cacatatgag ttagacagtc aatgcatatc agatatgcat
      361 tgtagaacgg attaaaatat cgtggatgaa attatagatg aattaaagga gacaattagg
      421 aatttatgga ggtaatataa tgagaacata taaaaataaa gaagaattaa aaaaagaaat
      481 aaataggact tatactaaat atattttaga atttgaaact attcctgaag aattgaagga
      541 taaaagagtt gaggaagtgg atagaacacc tgctgaaaat ttagcatatc aagttggttg
      601 gacgaattta attctaaaat gggaaaatga tgaaagaaat ggattatctg ttaaaacgcc
      661 ttctgatcaa tttaaatgga atcagttagg agagttatat aagtggttta caaataccta
      721 tgcacatcta ccattgaaag aattagagga aattctcaat cgtaatattg atgatattaa
      781 catgatgatt gattcaatga gtgatgaaga tctattcact gcacacaaaa gaaaatgggc
      841 agacgaggca accaaaacgg ctgtatggga agtatataaa tttattcatg taaatacagt
      901 tgctccattt gggactttta gaacaaagat aagaaaatgg aagagattag ctttgtaaat
      961 tcccgtttac tgctaagcaa ttgtggaaag gagacctctt gtatgttaac agtaagaaca
     1021 tacgactaca taagttcttt gctaactgcc ggtttttatt gggtgaatgc aagtgcactc
     1081 ccctagaatg ccgtagaggg tgcatcgtta gattgtatca attttggttg gccaacagat
     1141 aaaccatgac tcacttcgat aaaatgagat tatggtgcca tatccgctac aaagggactt
     1201 aggagactag caggggaaag atttttttat ttttgggatg tttttcagac cgccgatagt
     1261 accgacaatt ccctttaaat atggaatctc aacaacccac atgaagggta aggcaagtcc
     1321 ggtaattata tcttctgact ttgggttagg aagaattgaa attgttaaga acgattcaac
     1381 gcttatggtt tataatttgg agatagcctt tgttaatatg ctaaagccaa catacgatac
     1441 agattttgag cagtttattc taaccatcaa agggtatgtg gcctatcgcc tatcctatta
     1501 ctgatactga agagatctta aataaattct atacgggatt tgaagatcct gaaggtgaaa
     1561 tcgtcattta tcagaataaa taaatcaaat agtagaagtt caaagaatga ggggaacatt
     1621 agtttttatt gcttagttta atcccagtac cgatttctgc aagatgtaaa aggtctttca
     1681 tttcataagt tttcagaagc aagttcatac gcactaactc actttcgatt gagtagcaat
     1741 cgtcaacaaa ctttactatc agctgttatt tttattcttt cgtgttatct aaataccatc
     1801 aatttataag ttgtaggtgt atttaataaa agaacaaata actttagtat ttttattaat
     1861 ctaacttaca aactagtagt ataggggtaa atttgcgatt attcataaat ataccaagta
     1921 aaagtatagg gtatagtaaa aatatgataa aataaatagt cgttatctta atccaactat
     1981 atagaaaatt tacgttttct atatagttgg atgtttacaa gttatatctg gtttaataag
     2041 tttttttctg ttagtcatgg catgttttat tgtgctggtt catcaatata ttgaaatgta
     2101 ctaaaatgaa attttcagtt atggtataat atttatgctg tctgctaccc cggcctatta
     2161 tggcaagggg aggaggttgt attatgaatt accttttcga tatgtttttt gcaatcttag
     2221 cagaagtatc cgcgcattac atctgtaaat ggcttgattg caaaattatt gccaaaaaag
     2281 acagtaagcc tacccgttaa tctgaataga acgtaaaaga aaaccactag cccgccagct
     2341 agtggttttt ggtgtatcat gaattacctt ttcgttaaaa caattatacc atatttccta
     2401 aaaaatacaa ttaatagacg aattatcatt accactttga tgaaaaaagc tgacataaat
     2461 ttttctttta tttttataat gaataaacat ttatcaataa gcttttgaaa ggcaggatcg
     2521 tactttgttg atgattgaga tattgctaca tcaatctcag cagttaagat ttttttgaat
     2581 tgttgcttga attttttctt tatctaaaaa aattgtatct ccaggatgca acccattttc
     2641 tttaagaaat tccttttaaa ttgttaatac tactgagtgc cccacttctc tgaattttct
     2701 ttaacttctc cctccccttt tttatttgta tatacattca tatacgcttg tt
//
LOCUS       DACDPS010000168         2692 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.174, whole genome shotgun sequence.
ACCESSION   DACDPS010000168 DACDPS010000000
VERSION     DACDPS010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.174"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            257..532
                     /locus_tag="IWJ07_002736"
     CDS             257..532
                     /locus_tag="IWJ07_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334253.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            545..2617
                     /locus_tag="IWJ07_002737"
     CDS             545..2617
                     /locus_tag="IWJ07_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP9334254.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
BASE COUNT          827 a          422 c          576 g          867 t
ORIGIN      
        1 tttttgtcgc agccccattt ttttgttgtg ataaaagaac aagaaaggca ggaatgggaa
       61 agtaaacatc cttttgtcag aaacttttat ctttcttaag ttttacttga tatctgatac
      121 tatttggaat agaatgaggc gttgaaaaat ttatggaaat ttctaactag caataaacga
      181 tgaaacaatg aaatattttt catccaaaaa gaactttgct ataatgatgg aagtttgtgt
      241 ataggagatc tggaaaatga gagagagttt tgaacaacaa aaaaaactgt tgcatgatcg
      301 ctatggcgcc ctttcgatgg atgaccgccg tcaaatactt tgtaagttgc gtaaaagaaa
      361 tatattgatg tatcgtcagt tggaacgtct gaaacatgat ctgcttcgtt tggagtcaaa
      421 acgtgtacag tgtgaattag aggggaacca aacacaggtt gaagtagtcg aaaccaaaat
      481 tttaaagaaa aaagaacaat ttttaaaaat gcttacacaa aataaaaaat aaaggggatg
      541 gacgatggaa tttgctgagt tagttattgt atttgcagta acgatcacct tatcgaacat
      601 agctagccgc attttgccaa tgattccagc tccactgatc caaattttcc ttggagtggt
      661 attaggtctg acagagtggg gacaatcaat cgattttgaa cctgaattgt tcttagttat
      721 gattattgca ccattgttat ttagagaagg agaaaaagca gatatttctt cgattctcaa
      781 aaatttcggg acaattttgt tcttggcatt tggaggagtc attttgaccc ttgtcggggt
      841 aggtgcgacg ttatcattcc tgttgccaag tgtcccactt gctgcctgct ttgcttttgg
      901 agcagcgtta ggtccgacag atgcggtagc agtcagttct ctttctggac gagtgaatat
      961 ccctaaaaag gcgatgcata ttttggaagg agaaggtctt ttgaatgatg catctggtgt
     1021 tactgccttc cagtttgctc ttggtgcgct gatcaccgga tctttttcag cagtaaacgc
     1081 ggggatgagt ctagtagttt ctagtattgg gggagctttg atcggctttt tgcttgtctg
     1141 gttcaaacaa aaaataattc atttgatcga aaaagcatca gcacaagatg tcacagggta
     1201 tctgctgatc gaacttttgc ttccttttct agcctatgta ttggcagagg catttgatgt
     1261 atctggaatc attgctgcag tggcagcagg tattctccaa gcttctggtt ttaggaaaat
     1321 ctctgtgttt gatgcagaat tgtcgagctt gtcccacagt acatggacaa ctattgcttt
     1381 tacactgaat gcattagtat ttatattttt aggaatcgaa ttgacgcaag tgttctcgcc
     1441 agtatgggga gacggtttat atccaaacgg tcttttatta gcgattatcg tattaatctc
     1501 tgtcatgctt tttgtgatca gattcatttc tatcagtctt ttttatgtat tcaaagatgg
     1561 cagtaaaaag ttcaaaaaac agttgaacga gatcttgatc ttaacatttg gcggtgtaaa
     1621 gggaacagtc agtttggcta cgatttttat tttgccattt tcaatcaata atatgatgtt
     1681 ttaccagcgg tctttgcttt tgtttctgac ggcgggtgtc atcttagtga ctttggtcat
     1741 agggatcatt gtcttgccta tgctgacgga aacagaggaa gcgaaaagca cggatttgaa
     1801 tgctttgatg attttagaag aggtcgttga agttttgcga aaagaaataa aggaaattga
     1861 tcgtaataca aaagaatttt tagccactga agcagtgata gaaaattatc aagaacggat
     1921 acgtgatctg tacttggaag atttaaccga tgatgaaaag caagaagtac aagaaataca
     1981 agcactgatt ttgtcgatcg aacgggatgg attggatgaa agttatcgtt cgggcaagct
     2041 ttcttctaat ggttatcgtt tttactcccg ttttctttct cgattcgaac attctatcac
     2101 aagccagatt ttgtctttta tcggcttttg gttcattgtt gtccgtaggt taatgcgaat
     2161 cgttctccat ccgaaaatgt tttggcaaag aagatatgct gatcgtcaga catttattag
     2221 tgatgaagat atccaagaaa tcagagagac atatcaaaaa aatactcagt tgattattga
     2281 aagtttagat aacttgactg atatttatga tgaaacgatg gttcaatttt ttatccagca
     2341 acgcaaactt gaaggaataa aaatgaactc ggggaatttg atttcttcgt ggatgatcca
     2401 gcaagatagt ctgtttacga aaaaaatgct aagaggttat tatcttgaaa gaaaaatcat
     2461 tgatgaatac gaagttgctg aaaaaatcac gacgttttct gcgaacagct atcgcaggaa
     2521 tatcaatttg ttagaatctt atacgatgaa caaaccatct gataactttt ctttccgatt
     2581 tgcgtttcga gcaaagtcaa aaaaacgagt ccaataaaat aaaaaggtaa aaattttcaa
     2641 gttttataaa aaacaaaaca tatcagaaca ggatcgaatc ggtcctgttc tt
//
LOCUS       DACDPS010000169         2641 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.175, whole genome shotgun sequence.
ACCESSION   DACDPS010000169 DACDPS010000000
VERSION     DACDPS010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.175"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            56..1507
                     /locus_tag="IWJ07_002738"
     CDS             56..1507
                     /locus_tag="IWJ07_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP9334255.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1856..2488
                     /locus_tag="IWJ07_002739"
     CDS             1856..2488
                     /locus_tag="IWJ07_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAP9334256.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
BASE COUNT          993 a          379 c          516 g          753 t
ORIGIN      
        1 tatcataatt tttaatagaa aacactatta aagagaaaaa agagaggtaa atcaaatgaa
       61 aaaatcttta aaacttgcat cagcagttat attaatgtcc caactcctta tagcaacacc
      121 tttccatgtg ttggcaagcg aaaattcaac atacaaaatt gcaaaagaaa gaaccgagac
      181 gactgaaatg agtaattccg atgtaacagc agagagtagt gaacttttga caacttcatc
      241 aagtgaaagt agtacgactt caaatgccga gtcttcaaca acttcatcaa gtgaaagtag
      301 tacgacttca aatgccgagt cttcaacaac ttcatcaagt gaaagtagta cgacttcaaa
      361 tgccgagtct tcaacaactt cgtcaagtga aagtagtacg atttcaagta ccgaatcttc
      421 aacaacttca agtagtacaa gtgaaataac ttcttcgaaa gtgacaaaac caaaccatat
      481 agaatcttca aaaaaggaag cgccaaaaac ggaaggacaa acaaaaactt tttcgacacc
      541 acagcaaata gaatcaacag aatctttcca atcacaacca atactatctg gtaatgaaga
      601 aattcatttt gacaaaaatg aatctgtaga aaattttatt atgaaaattg gtgagtctgc
      661 gagaaaaatt ggtcagaaat atgatttata tgcttctgtt atgattgcac aagcgatctt
      721 agaatctgcc agtggtcaaa gccagttagc tcaggcaccg aattacaatt tatttgggat
      781 aaaaggaact tataatggaa actttgtcat tatggtaaca aacgaagatt tgggtaatgg
      841 aacgctttat acgacccaat caaaatttag agtgtatgaa aactatgagg aaagctttga
      901 agattatgcc aaattattaa ctaagggaat tagtggaaat aaagattttt atgcaggagc
      961 attaaaagct aatagtaaaa catatcgaga agcaactaaa ttccttacag gacggtatgc
     1021 aacagataca caatactatt tgaagctaaa tgaattaatt aaaacctacg atttaaccaa
     1081 ctatgataaa gaagttaaag gtccacaaat gagtaagact ggataccaag tgcctattca
     1141 taattattca ataaccagtt tgtttggaaa tcgtggaaat gaatttcacc gaggacttga
     1201 tatggcagct acacagggtg agccgattca tgcagttaaa gctggtaagg ttgttaaagc
     1261 agagtttcac tactcttggg gaaattacgt ggtgatacaa cacgaagatg gatcaaccgc
     1321 gttatatgct caccagcaac aatacctagt aaaaactgga gataatgttt tgcaaggaca
     1381 aataattggc tatgtaggaa gtactgggaa tagtactggc ccacaccttc atttggaaat
     1441 ttgcaaagat agtagtcttt ctcagagcat gttagttgat ccgaaaacct tattattcgg
     1501 agactaaaaa aggtaatcaa tctgacaaag taacaagaaa atggttggat tcttagacct
     1561 ttgatcagga tattgagact ggaattgata tctcgattac gatgggtttg acaaataaaa
     1621 caagtccatt atcgaatatc gagtgtatta ttgccgtctt tatgctcata ttttgagcag
     1681 gtttcactag atgaaaacaa tttatctatc ttgaggattt cgtgtacgta tcgttcagtc
     1741 ttgtattgta aattagtcat aaaatcagac taagaaaaat tggaaatatt tttttctgat
     1801 ttattaaaag ttaattgaaa ttatatggta cttctttctt gggagggtag gtgcagtgaa
     1861 ggaaagatat ggtcacctta ctgttgttga ggagattact cataatggat ataaaaaata
     1921 tcgttgccta tgtgactgtg ggaatgaaac gattgtatat cgatcaaact tactatcagg
     1981 tagaaccaaa agttgtggat gtggaaatta taaaaatagg cataaatata aaaatttgac
     2041 aaataaaact tttggtaagt tagttgcaat caagccaact aaactgcgaa gtgaaggaac
     2101 tgttgtttgg gaatgtcaat gtctgtgtgg gagaaaaaag ttagtatcgg caaagcgact
     2161 tctaaaaggt gatgtaaaaa gctgtggatg tttgaaatca aaaaagcatc acattgaaaa
     2221 taaaaaattc ggattactta ctgcaaagta tcctttggac aataactgtt catggaaaac
     2281 aaaatgggtt tgtcattgtg agtgtggcag tatgtgtgag ataagttata gtaacttatt
     2341 ttttggacat actcaaagtt gtggatgttt aaaaaggcaa gaaacaagga catgtgttga
     2401 agggacagta atagagtgtc taacgtcaaa attaccgaaa aataatacaa gtggtgttaa
     2461 gggagtttat cgttctaaag agaaatagat tgcgtatata actttaaaaa aaaagaggta
     2521 ctatttagga tcgtatgatt ctattaagag tgcagctcaa gctagaaaag atgctgaaaa
     2581 taaattgttt caacctttga ttgacaaata tcgaggtaat cattaattat tgtggataat
     2641 a
//
LOCUS       DACDPS010000170         2630 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.176, whole genome shotgun sequence.
ACCESSION   DACDPS010000170 DACDPS010000000
VERSION     DACDPS010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.176"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(61..2424)
                     /locus_tag="IWJ07_002740"
     CDS             complement(61..2424)
                     /locus_tag="IWJ07_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HAP9334257.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
BASE COUNT          700 a          553 c          483 g          894 t
ORIGIN      
        1 cttaatttga caaataatac aatgtaaaaa attctgtttt gcgggatcga ttttatcatt
       61 ttattggtca agatttttcc atttccaatc caatacttct ggcagatcaa taccattttc
      121 acgaatatag ttttgatgtt cttgtaattt ctgatccatt ttttccgcaa aatcttctgc
      181 ttgacttcct ttgattttta aagctgcgtc ttttgccaaa tgataacgat caagttcatt
      241 tactacacgc atgtcaaatg gtgtcgtgat atctccattt tctttgtagc catgaatcat
      301 taaatgatgg ttatgacgat caaagaaaat cgtgcggacc aatgtttcat aaccgtggaa
      361 agcaaataag atcggtttgt cttttgtaaa atattgatca aattcttcat cgctcaaccc
      421 acgcggatct tgacttggat gacgtaattt caataaatct acgacattga tgaaccgaat
      481 ttttagtttt gggaattctt ccagcagcaa agttacagct gctaatgctt ctaagttaga
      541 ttccgttcct gcagcagcaa tgaccaattc aggttcctcc tcttttgctg tcgaagccca
      601 atcaatgatt ttcaagcctt cattcactaa gacggctgct tcttctgctg agtaaaattg
      661 agcacgcgga tgtttggaag agatgatcaa attgattttt tctgacgagc ggaatgcttt
      721 atccatgact gcgagcaatg tatttgcatc cgcaggcaga tattcacgga tgaactccgc
      781 cttcttttct gccaaatgtg tcaatattcc tggatcttgg tggctatatc cattgtgatc
      841 ttgctggaag actgttgagc ttgcaatgag attcagcgaa ggataatctt ttcgccaaga
      901 aaggtcatgt gatttccgta tccatttgaa gtgctgagtc aacatagagt caaccactcg
      961 caagaatgat tcgtaacttg caaaaaaacc atgccgtcca gtcagaacgt acccttctaa
     1021 gaacccttca tcttggtgtt ccgataattg actgtcgatg acgcgaccag atggcgaaag
     1081 ccattcatca ttttctggtt cgattttttc catccattgg cgatttgtcg ttttgaaaac
     1141 ttgattcaat cggtttgatt ttgtttcatc tggtccaaat atacggaaat tttctggatt
     1201 tttctcaatg atctcgcgaa cgaatttacc aagttcggtc atatcttctg ctttaacgga
     1261 gcctggtttt tcaaattgca aagtatattt tttccaatct ggcataataa gcggtcttgg
     1321 atcgattcca ccattggtga taggattttt tgccattctt tggtcaccag taggcgcaat
     1381 ttctctgatt tcttctaaaa tacgtccttg tgcatcaaat aatttttctg gttcatagga
     1441 tttcagccaa cggagtaaag catctgcatg atccatatgt tcttgatcta caggaatcgg
     1501 tacttggtgt gctcggaagg ttccttcaat tggttccccg tcccattctt ttggaccggt
     1561 ccatcctttt ggtgtgcgta cgatcaatac tggccatttt ggcatcacga catctgttgc
     1621 tgtcttcagg cgagcttctt tttggatgga tttgattttc tcgattgctt gatccatttt
     1681 ttcagccata acaggattta atttttctgg atcatttcct tcaataaaga aaggctccca
     1741 gccaagtccg ttaaagtaat ttgctaattc tccatctgat ttacgtgcca agatcgttgg
     1801 gttggctatt tttgcaccat ttaaatgaag gatcggtaaa acagctccat ctatcactgg
     1861 attgataaag acatttgaga accagcttcc agctaaagga ccggtttctg cttcaccatc
     1921 accaatcacg acagcagaga ttacttcagg attgtctaat acagctccga ctgcatgtga
     1981 aagagagtat ccaagctctc cgccttcatg gatagaacct ggtgtttctg gtgctgcatg
     2041 agaagcaatg cctccaggaa atgaaaatcg cttgaataat ttttgcattc ctgtctcatc
     2101 ttccgttact tctggataga tctctgtgta acttccatct aaataagcat tcgataccat
     2161 gacttgtccg ccgtgtcctg gcccttcgat ataaaacatg ttcaaatcat atttattgat
     2221 gactcggttc aaatggacat aaataaaatt ttggccagga atcgttcccc aatgaccgat
     2281 tggatgtttt ttgacatctt ctggtttcag tgtacgtctc agtaaggggt tatctttaag
     2341 ataaagttgc ccaacagaaa gataattagc agcacgccac caagccgtca ttttatcaaa
     2401 gtattcttta ctagaataat ccatacataa cactcctttt cattattact cttattctag
     2461 cgcagatact cccaattgaa acctaataag cacttctgtt catttgtttg gattttatat
     2521 ctagccaaat gaacaggtat aaaaaaccac gaaatttttc ctttcgctga cgttttttac
     2581 attgatccaa aaaacttgtc gttatcacaa tggctgttcc gcagttcatt
//
LOCUS       DACDPS010000171         2550 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.177, whole genome shotgun sequence.
ACCESSION   DACDPS010000171 DACDPS010000000
VERSION     DACDPS010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.177"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            561..1472
                     /locus_tag="IWJ07_002741"
     CDS             561..1472
                     /locus_tag="IWJ07_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334258.1"
                     /translation="MKYLLHGYFIDYSTRNSSTLAILCLLHELRVVTRKQLYKLLNFD
                     RSISKQGVTNALVRLYEKQLIDRKKYGTEMYYYLTKEGHQSIGGYYTLPKVPEYNLNH
                     HLQINNYLIKGMALAKDHPHLKRIISERRTVYETKDLNPKTKGRKFFVPDFIFRFRDK
                     ENHEINWSFEIELTLKTRRRYREAIFPKYIRELRTRPESHLIYVTPSGAIQEELHAFK
                     RIFFKRETEKDASQQELFERLHIFSALEFEEGLKALLENDPAVNWRTVEREKIYPNGA
                     VQSRLNLFEESTENEEKPKVKFTPEVL"
     gene            1959..2225
                     /locus_tag="IWJ07_002742"
     CDS             1959..2225
                     /locus_tag="IWJ07_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334259.1"
                     /translation="MTTKNEQLKEQARKELQERGLIIEGSFEGDFETYIGCYARPINK
                     PTALDPMNEQEVKEQEKHAVNGFPQDFTEWYEWEINNGKLENFL"
     gene            2297..>2550
                     /locus_tag="IWJ07_002743"
     CDS             2297..>2550
                     /locus_tag="IWJ07_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334260.1"
                     /translation="MAKNTPYQVGDIFYSSWGYEQTNVTFWQIVKLTEKTAWFQPIQK
                     KTVEHYSSMSGKVVPMKDKFIEYPLAKTKDSIVKKRIDPET"
BASE COUNT          928 a          440 c          556 g          626 t
ORIGIN      
        1 tggaaaaacc gccacttatg agagaaaatg gtcaaaaacc taagcgggaa gtctccgaat
       61 aacataccaa gcgccgacag ccaacagata aagaatacac tactaaaaat agtagtagca
      121 taaaatttaa aagggaaaaa atcaacccct accccagtct tggggtctca aaactaactc
      181 ccccctaacc ccctctttca gcctcttttg aaagaggggg agaatcaacc ccacggccaa
      241 cgccccagtg ttcttggggc tgtccagaac agaaacttgc gagagagtgg gggtaaaaca
      301 agtgtagagg aaacttgaag acttgttcga acagaaactt gcgagagagc cggagtaaaa
      361 caagtgtaga ggaaacttga agacttgttc gaacagaaac ttgcgagaga gttggagtaa
      421 aacaagtgta gaggaaactt gaagacttgt tcgaacacaa acttacaaga gaggggagta
      481 aagcagttgc agaggaatca ggaagatttg ttcgaacaga aacttgcaag aaagcattat
      541 taaattgaag gtgatgaaat atgaagtatt tattgcatgg ctattttatt gattattcta
      601 ctcgcaatag tagtacttta gcgattctat gtttgcttca tgaattgaga gtggttacta
      661 gaaaacagct atacaaacta ttgaattttg acagatcaat tagtaaacaa ggagtaacga
      721 atgctttagt gagattatat gaaaaacagt taatcgatcg aaaaaaatat ggaacagaaa
      781 tgtattatta tttgacgaaa gagggacatc aaagcatagg tgggtattat acgttgccga
      841 aagttcctga atataatttg aatcaccatt tacaaattaa caattatttg attaaaggaa
      901 tggcattggc aaaagaccac ccacatttaa aacggattat ttcagaaaga cgtaccgtct
      961 atgaaacgaa ggatttaaat cctaagacaa aaggaagaaa attttttgtg ccagatttta
     1021 tttttcgttt tcgagacaaa gaaaatcatg agatcaattg gtcctttgaa attgagctaa
     1081 cgttgaaaac acgtcgtcgc taccgtgaag cgatttttcc gaaatatatt cgtgagttgc
     1141 gaacacgtcc tgaatcacat ttaatttatg tgacacctag tggcgcaatt caagaagaac
     1201 ttcatgcgtt taagcgtatt tttttcaagc gcgaaactga aaaagatgcg agtcaacaag
     1261 aattgtttga gcggttgcat attttttcag ctttagagtt tgaagaggga ttaaaagcat
     1321 tattagagaa tgatccagca gtcaattggc ggacagtaga acgagaaaaa atttatccga
     1381 atggcgctgt tcaaagtcgt ttgaatttat ttgaagaatc aactgaaaat gaggaaaaac
     1441 caaaagtgaa gtttacgcct gaagtattgt aaaagagggt gttcgcttcg ctcttttttt
     1501 gtgccagttt gctggcaccg ttcagacgca agcgtctgaa ccggctcgtt tttaggaagg
     1561 actaacttgt ttgatttatc cgttgtcaaa gaagtggacg ttcgagagtc cctaatcgta
     1621 tagcagtcat tcggcgagta taacctaaaa cataacctgc caattgccaa gggtaaaatg
     1681 cacgctgcgc gcccttggca atcgtcagaa catgttttcg agccggttat gccgaacgac
     1741 cgctatacga aaagggcctc taaacagaag tccactttga tccttgacaa ctgaaaccca
     1801 aaatcaaaca agcaggcctt cgtctaaaaa cgagccgaat gaagaccttg ggggttgcac
     1861 ccccaaaccc ccgtgccccc gacacactac cgaaaaggtg ggggtgggtg gcgaaaaaat
     1921 caaataaaac attctattca agaaaggaag aaacaaccat gacaactaaa aacgagcaac
     1981 tgaaagaaca ggcaagaaaa gagttgcaag aaagaggttt gattattgag gggagctttg
     2041 aaggagattt tgaaacctat attggttgtt atgcaagacc gataaacaaa cctacagcct
     2101 tagacccaat gaacgaacaa gaagtaaaag aacaagaaaa acatgcagtg aatggttttc
     2161 cgcaagattt cactgaatgg tatgagtggg aaattaacaa cgggaagctt gaaaacttct
     2221 tataaaagtc agatgacgtg caaagagaat gattctcttt gtacggtaaa aatataaaga
     2281 aagaaggaaa aagaaaatgg caaaaaacac accttatcaa gtaggagaca ttttttatag
     2341 tagttggggc tacgaacaaa cgaacgtgac tttttggcaa attgtgaagt taacagaaaa
     2401 aacagcatgg tttcagccta ttcaaaagaa aacggtcgag cattattcgt ccatgagtgg
     2461 aaaagttgtt ccaatgaaag acaagtttat agagtatcct ttagcaaaaa ctaaagacag
     2521 tattgtaaaa aaacgaattg atccagaaac
//
LOCUS       DACDPS010000172         2519 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.179, whole genome shotgun sequence.
ACCESSION   DACDPS010000172 DACDPS010000000
VERSION     DACDPS010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.179"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..460
                     /locus_tag="IWJ07_002744"
     CDS             <1..460
                     /locus_tag="IWJ07_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334261.1"
                     /translation="NEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMT
                     EKELINTVCERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFL
                     IYLAKGAGIDETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(536..871)
                     /locus_tag="IWJ07_002745"
     CDS             complement(536..871)
                     /locus_tag="IWJ07_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9334262.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            998..1213
                     /locus_tag="IWJ07_002746"
     CDS             998..1213
                     /locus_tag="IWJ07_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9334263.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            1250..1612
                     /locus_tag="IWJ07_002747"
     CDS             1250..1612
                     /locus_tag="IWJ07_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAP9334264.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            1658..1984
                     /locus_tag="IWJ07_002748"
     CDS             1658..1984
                     /locus_tag="IWJ07_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334265.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
BASE COUNT          880 a          349 c          496 g          794 t
ORIGIN      
        1 taacgaaaaa gaggctaaaa gagtatttcc tcaaaattcc cgatcagaac tcagcaagga
       61 aaaacaattg attttagatg aagcacatga agttttacag ggcttatcgt ggcaagggac
      121 atatctaatg acagagaaag aacttattaa cactgtttgt gaacgcctac gtaatgttgg
      181 ggataacagc gtgaaaaatg ctttaataaa aaacagaggc tactttgtta aacaaggatt
      241 cactcttgaa cgagcttcta aggatattaa gaaactatac aatgtgaaag caagtagtag
      301 ttttttaata tatttagcga aaggggcagg gatagatgaa acaataaaag aattgatatt
      361 atcaggagct gggatagttc ctctgtatca atttacaagt gtaacaagag atgatggacg
      421 gagagtaagg aagagaaaag acccaattgt attaaactag acaggttatc catagctctt
      481 aatcttattt catctcaata cacccaccgt taaagttcgg tgggttattt atttttcaat
      541 ctttcaatag ctgattgcgt tcattttctt gcctttttaa tagcttcata tagtcaattg
      601 ttcgaactgc atcttcagtg ctaagagaac gatagagagc caaaaactct tcctcataat
      661 ctacgacaac attgtctaga aaatggctta caggggttcc tagggctcta gaaagagctg
      721 ttaagctatc gaggttaggc tctgttacag aattttccca tcttgctact gcctgttgtg
      781 tagagaagat tttttcagct aattcttttt gtgtccaccc gagagctttt cgcttttctc
      841 taatgacctt tgcatattgt ttctgtttca ttaatacacc cccatctcag tatagtacaa
      901 caagttgttg tatttgtaat ggtatttgaa gttttttata ttttatgagt tgaaatacat
      961 catattgttg tatataataa aacaggaggg gagaatcatg aaaaaagtaa gtaatctcag
     1021 ccgatacaga aaagaaaaag gactttctca aacggagtta gcaaaaaaaa tgaatgttac
     1081 acagcaatgt gtaagttcgt ggcaaacagg gagaacgatt cctaaaccct atcaaatgaa
     1141 gatgctttct gagatattaa gtgtaccaat gaatgagctg ttttctgatg tatttaataa
     1201 agtaaacagt taactaataa gcaattgaat gattttagga ggagaagaca tgtattgtag
     1261 aaaatgtggc aaagaaataa cagatgaaag taagtattgc cagttttgtg gagcactaca
     1321 agaaacagat gagacaaccg gaacagacac agtaaagaaa agtactgagc agacaattaa
     1381 tgaaatgaag gaacaaatcg tacatggaca agagacaggg aagctgtatt tgattattgg
     1441 ttggatttct atggtagtat cgttagcatt tattcctgtt gtatttggtg ctgttgctgt
     1501 tattatgggc tacttatatc gagaaaaaga tgagaaaatg ggtacgattt tgatgatagc
     1561 aggggtagct ggagcaatct ttggtgtact tttagggatg tcaatgtact gataagaggg
     1621 ataagaacaa attattttgg agataggaga taaaaagttg aataaagtaa atatgaacaa
     1681 gaagtttgtc attataggta ttgttgtctt ggctattata ggtattattt tttcaaaaat
     1741 gacaggtaca tctggattag agaagtcatc aaaaccattg gtaacacaga ttttacaaga
     1801 acagtacaac ttaaatcgat catgtgatga tgtgacgatt actcatgaaa atggagataa
     1861 cacataccgg gctaaagcta tattagacaa tggaagtgct atcaacataa atattgagta
     1921 ctatccaaag aaagaccgta tatatgtgga gataccatat gctgaggtgt taatgttaaa
     1981 ttgatttaaa tgtattatgc ataccgtcca aacaggaact cccaaaggtg taaattcact
     2041 agggagtttt tatagtttcc tataaattaa atactaatac aggtgacaaa aaaatattac
     2101 aatgattgtc gggaattttt atgttattca ctgttataac aatataaaag aaatgaaaaa
     2161 gaaaaaatta ttatggatat gtttggtatt tttattgata attggtggaa ttgaaatact
     2221 tactacatgt agattagaaa taaatcagtt taaacatttg ctagttaatt tttaagaata
     2281 ctagcccaaa tcattctatt gataccttta tatctcggaa tgattgaaat tggctattgc
     2341 ttactgattt ttaccaaaaa aacaagtatc actattgttg cctatattgt tggaatgctg
     2401 tttattccat cctttactca ccaaattcat caatttttcc caagagcaga attgttgaag
     2461 ttgtgtgatc cgttatatgc atttttgatg ctttcacgat tttgggaatt tcccgctgg
//
LOCUS       DACDPS010000173         2489 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.180, whole genome shotgun sequence.
ACCESSION   DACDPS010000173 DACDPS010000000
VERSION     DACDPS010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.180"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..922
                     /gene="yidC"
                     /locus_tag="IWJ07_002749"
     CDS             <1..922
                     /gene="yidC"
                     /locus_tag="IWJ07_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP9334266.1"
                     /translation="WKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVHPMG
                     QAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVEAAQ
                     QKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARYTEG
                     IRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAMIVF
                     MSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPRKDV
                     TESAKPADNKQPKNSTNNKGRNAGKQQKRK"
     gene            1025..2373
                     /locus_tag="IWJ07_002750"
                     /pseudo
     CDS             1025..2373
                     /locus_tag="IWJ07_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012008915.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
BASE COUNT          866 a          456 c          479 g          688 t
ORIGIN      
        1 atggaaaaac tggttattgg gatccggact tgtcagtatc ctgctctttt tatctggctg
       61 tgtgcggatg gattcaaacg gaaaccctga cacttcagga atcgtttatc gagtactcgt
      121 ccatcctatg ggccaagcga ttacatatct agttgagaat ttcaattggt cttacggctg
      181 ggcagttatt gcaatgactg tgatcgtccg tatcatcatc ttgccactag gtatcagtca
      241 atcaaaaaaa acaatgatcc aatctgaaaa aatgcaagcg ttaaaaccac aagttgaagc
      301 agcacaacaa aaactaaaag ctgcaacaac acgtgaagaa caaatggcag cacaagcaga
      361 aatgcaacaa gtatatcgcg aaaacggatt gagtatgacc ggcggaatcg gctgtctgcc
      421 tctattgatc caaatgccga tcttctctgc cttgtatttt acggctcgct acacagaagg
      481 tatccgtgaa tcatcttttt acggcatcga tctaggctca ccaagtatgg tgcttgttgc
      541 cattgcaggg gtcgcttatc tgcttcaagg atatatttca acaatcggta ttcctgaaga
      601 acagaaaaag acgatgcgta cgatgctgat cgtctctcct gctatgatcg ttttcatgtc
      661 gatcagtgcg ccagccggtg ttacacttta ctgggtagtg ggtggtgtgt tcagttgtct
      721 gcaaacattc atcacaaacg ttatgatgaa accacgtttg aaagcacaag tggcagaaga
      781 gttgaaacaa aatccgccaa aacaagtcgt cactccaaga aaagacgtga cagaatcggc
      841 aaagccagct gacaataaac aaccaaaaaa cagtacaaac aacaaaggcc gcaacgcagg
      901 aaagcagcaa aagcggaaat aaaaatatat gacaaaaaac tgtagacaaa taaaaaaatt
      961 tctttgttct acagtttttt tgttacaatc aaagaaaacg tcgcttttcg aggaggaatg
     1021 tataatgatg tccaaataat caatggatag tcgaatggaa gaagccaaaa tttcgctaga
     1081 tgcacttgtt cctaaaaatc atttggtcca taaaattgac aaaatattgt tatttgattt
     1141 tatttatcca attgttgaat caacttactc gaccattggt cgtcctagcg ttgatcctgt
     1201 catcttagta aaattagtat tcattcaata tttatttagg attcgttcga tgcgtcagtc
     1261 cattaaagaa gctgaaacaa acctagctta tcgttggttt cttgggctta gtttaatcga
     1321 taaaattcct catttttcaa cgtttggtaa aaactatgta cggcatttca gagaaaccga
     1381 ttgtttgaac agatatttat cattatcctt gaacaagcag ttgaagctag atttattcat
     1441 gaagaaattt tgtatatgga ttcaacctat attaaagcga atgcgaaaaa acatattttt
     1501 acaagagaaa tggtacataa ataagccatt ttataccaaa aacaattgaa agaaaaaatt
     1561 aatgaagcaa gaattgcaga aggcaaacgt ccttttggtt caatcattca tcaggaggtg
     1621 gtagaaggga aaattagtgt cgcagatcca gaaagtggtt attatgtaaa aacagaacaa
     1681 gaaaaacagt ttgcttattc tgctcatacc gtttgtgatg aaaatggttt tgtattagat
     1741 gtcatgatta cgcctggaaa tctacatgac agccgaatgc tcgtgccaca aatcgaacgt
     1801 gttaaatcat gttgtggtgt agctgctgat gctgcgtaca aaacaccttg gaacgccaag
     1861 tatttaatcg atcgaaaact acgacccatt tttccttata ctcgacccaa aagaagcaaa
     1921 gaacgtttta aaaagaaaga tttttattat gatccctatt ataactggta cctatgccca
     1981 aatgatcaaa cacttcagtt tcgcacaact actcgagatg gttataaaaa atatgtatct
     2041 aaatctttta tttgtgaaag ttgtctatta ttgaaaaatt atacagaaag ccagaaacac
     2101 cagaaagaaa tacatcgaca tatttaggaa gactacttag atgaagcaga acatttacgc
     2161 catacggtgt acaatcgcga aaaatataaa aaaaagaaaa gaaacgattg aacgcgtttt
     2221 tacagacgca aaggaaaagc atggaatgcg ctacaccaaa tatcgaggct tggaaaaaac
     2281 caggtgcata cgatgcttgt ttttgccgca atgaatctgc aaaaattagc catttggcag
     2341 tgggggcata tgtctttata cccgtggtgc tagttaattt tttcctagag gaaaatgtct
     2401 aaacatgttg gtaaatcaac aaaaaaacgc atatagtagt ctcaaaactc ccaagaaatg
     2461 aggactacat atgcgttcat caaaatata
//
LOCUS       DACDPS010000174         2438 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.181, whole genome shotgun sequence.
ACCESSION   DACDPS010000174 DACDPS010000000
VERSION     DACDPS010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.181"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(100..300)
                     /locus_tag="IWJ07_002751"
     CDS             complement(100..300)
                     /locus_tag="IWJ07_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9334267.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            complement(923..2224)
                     /locus_tag="IWJ07_002752"
     CDS             complement(923..2224)
                     /locus_tag="IWJ07_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9334268.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          733 a          436 c          451 g          818 t
ORIGIN      
        1 ttttatacac tactattcag cccattcaac gaccaatgaa ttttttcgta caaaaaaacc
       61 gtcttttctt tcgaaaagac ggttaaaagt caatttttat taagctttaa caatgtttgt
      121 tgcttgaggg ccacgttgac catcttcaac atcgtaagac actgcttggc cttcttctaa
      181 agttttgaag ccgtcacctt ggatagctga aaaatgtgca aatacatcat ttccatcttc
      241 accagtgata aagccaaaac ctttgtctgc gttgaaccat tttactgtac cgttattcat
      301 aaataaattc ctcctgatac gtatcaatgt acgttttttc gttgcaataa atatttgata
      361 ggcaaaagga gttttatcta tgtagatata tcgctcaaat tacgtttcaa aatagattac
      421 tcatttaata tatcatgctt tttctaataa cacaaggata atgcaatgaa atataaaatt
      481 cattgtcttc tattgtcttc gaatcgaaga aaaagttgaa taaccgcgat tgattcactt
      541 tttgttttct tgtttatttg cttttatctg caacttttga ctttaaaatt tcttaactat
      601 gaaaaccttt caattggtgg tatacttata aatggaattt cgttatcaac gcgttcgttg
      661 cctttcagca agtatttaaa gaaaggagaa tggctatgac attctataac aaagatatac
      721 aatatcgagt aaccttggat acagatttga atctatttgt cgtttttgac aaagaaaata
      781 ccaatcatgt agcgacaggt gttacgatcg aacaagcagt gcaagaatta aaaaaatctg
      841 cataaggagt ggaaacggct ctttgtcaaa taggactgat agagtgagat aagaacatct
      901 aaggtagaat tccttttcta ctttagatgt tttttgattc ataggtaatc agattattta
      961 gtaagaaaat tctggtcttc attttccgaa agcttcgaaa gccataagcg actcgtttca
     1021 atgctttgat gtgggtatgc agattttcta ttttggcatt tgaataaggc aattcgaggg
     1081 ctaaacgaat gccggattca tagtttataa aagctttttt tatagaatgc agttcttttg
     1141 gaacggaatt gggcatcgtt cttacaagtt taaagaaagt gtccgcctct ttgtcgtgaa
     1201 acgcttgaag aatttcttga tagtaaaaat aggcgattcg taattcgtca gagatcgaaa
     1261 gcagacgatc caccacttca ctttcggtga gatacgccca accaaaattg cgccatttgg
     1321 tccatttgtc ataaacaagc ttgtcctgtc gtttgatcag aagccgagac aacgccttta
     1381 attgtcgata atatttggct tgtgttggat cgtttcgatc aaagcttttc atgattcgta
     1441 cgcgaatatc ctcaaattta cgaatgagat gtttgacaat gtgaaaacga tccacaacga
     1501 gttttgcctt tggaaaacat tcctttatca gagaggcata agaaacattc atgtccgtca
     1561 cgatgacctt taccttcaat cgtgcgcgtc gttcaaattg cataaaataa gaaacgagag
     1621 cctttttctt ccgtgaagga agaatatcca gtaattttcc agtatcgcca tccatacaag
     1681 aaaaggagta gggaccgact tgatttttca gtgaacgaaa ttcatccatt aataaacaac
     1741 gcggtaacca atttcttttt acttttgtca tggggatcag agaagcgagt gtccgagaaa
     1801 cggtcgacca agaaacgccg caatgcttag caattgtcgt cattgcttgt ttttcagtta
     1861 gctcttctaa aattttatag cgcacttgtt tagcgataaa gcagtttcta tcgacaatgt
     1921 aggtattcgc cgtaaaatag gtgtggcagc cacgacagtg gtagcgttgt ttcgctaaag
     1981 caagataagt cagttggttc gcacatttat taagttgaat gagcgatttt ttctttccgt
     2041 tcttaacgat catgccttca tgattcatct gtttacacaa aggacagcga cgaatttctt
     2101 tacttaacgt cgcagaaaca acaagactct tgatattctt aatgctcttc gattccacat
     2161 ttgtgatgaa aatgttttta tctttgattc caagtagtag tttgatatga tgaagatcag
     2221 gcatacagaa cgctcctttg attgtgttgt ggtgattcaa ttctaacgga gcttctgtat
     2281 gttttgtttt ttcatgctca aaagggaccg atagaatgat tttctatcag tcctagaaat
     2341 tatagagccg tggaaaccct catatggacg attctcgttc ttctctttaa acgcatctac
     2401 tggtagataa cagtagatgc gttttgtgtt tgttctaa
//
LOCUS       DACDPS010000175         2391 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.182, whole genome shotgun sequence.
ACCESSION   DACDPS010000175 DACDPS010000000
VERSION     DACDPS010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.182"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            197..2059
                     /locus_tag="IWJ07_002753"
     CDS             197..2059
                     /locus_tag="IWJ07_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9334269.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2059..2391
                     /locus_tag="IWJ07_002754"
     CDS             2059..2391
                     /locus_tag="IWJ07_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9334270.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          861 a          353 c          465 g          712 t
ORIGIN      
        1 aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt ttaacagtac
       61 atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct ataagttgca
      121 cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta tattagcaat
      181 gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt tgatttactt
      241 ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa atgttagtgt
      301 tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc ggaagcaagt
      361 cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg atcgaatcaa
      421 attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc aaagtctccg
      481 aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg gaatgaaaat
      541 ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg tgctggaaaa
      601 taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt tttggatcat
      661 aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag agttcacgga
      721 ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg aatactttga
      781 aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa tcattcgaat
      841 gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat tactaaatat
      901 tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga ttggtcgtta
      961 tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg tcatgcagca
     1021 ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata tctatagtga
     1081 tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg tgatttataa
     1141 agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga cgaatctgta
     1201 tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat ataatccttt
     1261 taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc aagcagttcg
     1321 acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat atattgcctt
     1381 acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag ccgtctacat
     1441 ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg aagaattaaa
     1501 taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga ttgctaccaa
     1561 agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc ctttcatcaa
     1621 agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa taaaaaaaat
     1681 acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa cgtccatcaa
     1741 taaagatact ttagaagact ttagcaagga attgattttg aaagcttata cgatttatga
     1801 acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag cttttttgct
     1861 tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg cagaaggtcg
     1921 tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg aagcagtttt
     1981 tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct catatataaa
     2041 agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat cgaggaaagt
     2101 caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt agtagaacat
     2161 gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa tgagatgctg
     2221 aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt caaagaagta
     2281 tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa tttaaccgat
     2341 gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta a
//
LOCUS       DACDPS010000176         2341 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.183, whole genome shotgun sequence.
ACCESSION   DACDPS010000176 DACDPS010000000
VERSION     DACDPS010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.183"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..600
                     /locus_tag="IWJ07_002755"
     CDS             <1..600
                     /locus_tag="IWJ07_002755"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9334271.1"
                     /translation="TERSSAPYKICQKNCCHSIISKENLLNQDFSTETICEKWAADIT
                     YIPTKKNGWCYLSSIMDLHTKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHT
                     DLGSQYTAREVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEE
                     ANRALFSYIEGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
     gene            complement(1073..1645)
                     /locus_tag="IWJ07_002756"
     CDS             complement(1073..1645)
                     /locus_tag="IWJ07_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9334272.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1661..2095)
                     /locus_tag="IWJ07_002757"
     CDS             complement(1661..2095)
                     /locus_tag="IWJ07_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HAP9334273.1"
                     /translation="MYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEI
                     IKKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPIND
                     LEIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            complement(2169..>2341)
                     /locus_tag="IWJ07_002758"
     CDS             complement(2169..>2341)
                     /locus_tag="IWJ07_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP9334274.1"
                     /translation="RPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSV
                     CTEIKVLLGIPA"
BASE COUNT          743 a          460 c          380 g          758 t
ORIGIN      
        1 acggaaagaa gttctgcacc ttataaaatt tgtcaaaaaa actgttgcca ttccatcatt
       61 tcaaaagaga atctcttaaa tcaggacttt tctactgaaa cgatctgtga gaaatgggca
      121 gctgacatta cgtacattcc tactaagaaa aatggttggt gttacttatc ttcaatcatg
      181 gatttacaca cgaaaagaat tattagttat acattttcaa aacgaatgac tgtggattgt
      241 gtcattcaaa cattgaataa agcaaaaata cattatcaca ttccagaagg aatgattcta
      301 cacactgact tgggcagcca atacacagca agagaagtgg aacaatggct taaaaccaac
      361 aaaataaggc attcttatag tagaaaagga acaccttatg ataatgctgg aatcgaatct
      421 ttccatgcct cattgaaaaa agaagaagtc tacacgacta gctactcaga ttttgaagaa
      481 gcaaatcgag cgctatttag ctacattgaa ggattttata accgaaatcg aatccatagc
      541 tcgattcact atctaacccc acaggaattt gaagaattag caaaagaaaa aatggcttaa
      601 ccgtctctac taaaatgtgt ccaagatatt gactcaaatc cagtaatatc acaataggaa
      661 tgctgtttat tatattagca gcagtgctga tttttatgat agttatttgg ttcttaaatg
      721 aaagatctaa agacaatcaa caccgtaggg atatggagat aaaatcaata gagtcaatag
      781 attatttatc gataataaca ctaaaaaagc cacgttccta aacgtgactt acgctgaacg
      841 atatgtgttg tttggtagac gaacaaatcg ttcattataa atatatgatg gtatcctaat
      901 atagtaagcg tggctagcat acctttaatt actttacctt ttacagttac catacacatt
      961 ttttgagcag aaatcaagta aataaaaaaa tattatattt cacaacatgc taatagactc
     1021 atatctagaa cgcattaagt atttacctgt ctggttcgct gagacttctt ttttaacttt
     1081 cttcaatttt cttctttatt ctaaaaagcg tactgcgact gaaaccggtt atagcttcaa
     1141 cttccttata cgatttacct gaagtaagca gttcatatgc gtgcctcttc ttcggtgtga
     1201 ttgttgcttt cggacgtcct tctctgaaat taggattatt ctttcttgca aacatttttc
     1261 cttcttgggt acgttccaca atcatatcac gttccatttc tgctacactc aacaacgtcc
     1321 gtgtcaccat tcgccccatg gacgtatttt caatcgttcc tagattcaaa accttgatcg
     1381 ttacgttatc atcgagtaaa ggttcaatga tctctaatgc ttctctcgtg ttacgagcaa
     1441 atcgatcaag cttcgtaaca actatttcat ctccaggctt cagaccagcc aacagctgat
     1501 tgaattttgg ccgatcccgt tttgtacccg taaatttttc tgaaaaaata ttcttcttct
     1561 ctacgcagtt ctcaagcaac tgttgaattt gttcttttgt actttgagaa gcggttgaga
     1621 cacgtgcata tccatatttc gtcatttggt ccacctactt ttacaaatca tctatcgtat
     1681 actcagaata cccttcatag aaatagctga catcgattcc tttcatatat aactcgcgat
     1741 cgtcaatttt atcagtcaga gcattggaaa ttagatagcg tatttccaaa tcgtttatgg
     1801 gacttcgctc cattgcagaa agatagtctg ctttatcgat taagttccaa tctacaactt
     1861 tttgcaattc ttcctttaaa atgagatcca gccaaattct agtacttcgg ccatttcctt
     1921 ctcgaaatgg gtgagcgata ttcatctcca catatttctt gataatctct tcaaaagaag
     1981 tttgtggcat ctgatcaata tgctgtaacg aatgttctaa atacataact ggtgcaaatc
     2041 gaaaatttcc ttttgcaata ttgaccgatc ggatttttcc ggcaaaatcg taaatatccg
     2101 aaaatagaaa ttcaggttct gttgcaaagt tttaaataaa gaataaaatc ccttacggta
     2161 tctatgattt aagctgggat tcccaataat accttgattt cagtacagac cgaaaacccg
     2221 aagagagtgc cttcttttcg ggttttctta tataatcctc gaatggcttc catgccttta
     2281 atcgtggtag aggcagtgcg taaacttcga tagaatttat tgcgtctctt tactggacga
     2341 t
//
LOCUS       DACDPS010000177         2250 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.184, whole genome shotgun sequence.
ACCESSION   DACDPS010000177 DACDPS010000000
VERSION     DACDPS010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.184"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..985
                     /locus_tag="IWJ07_002759"
     CDS             <1..985
                     /locus_tag="IWJ07_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HAP9334275.1"
                     /translation="YYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKALMASM
                     LEMYVSGVSTRKVSTIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKNYPYLM
                     TDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEYLKERG
                     LQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFREAVKGI
                     FKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRLKSTNL
                     IERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
     gene            1042..1434
                     /locus_tag="IWJ07_002760"
     CDS             1042..1434
                     /locus_tag="IWJ07_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9334276.1"
                     /translation="MEEVLIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            1435..>2250
                     /gene="aac(6')-Ie"
                     /locus_tag="IWJ07_002761"
     CDS             1435..>2250
                     /gene="aac(6')-Ie"
                     /locus_tag="IWJ07_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ie"
                     /protein_id="HAP9334277.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSIL"
BASE COUNT          863 a          292 c          398 g          697 t
ORIGIN      
        1 ctattatgag cgcagcttta cgacacgtgt aggcacgcta gaattaaaag tacccagaac
       61 acgtgatggc catttttcac ccacagtgtt tgaacgttat caacgaaacg aaaaagccct
      121 catggcttca atgttggaaa tgtatgtatc aggcgtttca actcgtaaag tatcaacaat
      181 tgtggaagaa ctttgtggta aatccgtctc taagtccttc gtttctagct taacagaaca
      241 gctagaacct atggttaacg agtggcagaa tcgtttatta tcagaaaaaa attatcctta
      301 cttaatgacc gatgtactct atataaaagt acgagaagaa aatcgagtac tctcaaaaag
      361 ctgtcatata gcgattggaa taaccaaaga tggcgaccgt gaaattatcg gcttcatgat
      421 tcaaagtggc gaaagcgaag agacctggac aacatttttt gaatacctaa aagaacgcgg
      481 tttacaaggt acggaactcg ttatttctga tgcgcacaaa ggattagtct ctgccattag
      541 aaaatccttc accaacgtaa gttggcaaag atgccaagtt cacttcctaa gaaatatctt
      601 taccaccatt cctaaaaaaa attcaaaatc tttcagagaa gctgttaaag gaatttttaa
      661 gttcacagat attaacttag cgcgtgaggc taaaaatcga ttgattcatg attatatcga
      721 tcaaccaaaa tattcaaaag cttgcgcatc attggatgat ggattcgaag acgcctttca
      781 atataccgta caaggaaatt cccacaatcg actaaagagt accaatctaa ttgaacgact
      841 gaatcaagaa gtacgcagaa gagaaaagat tattcgcatc ttccccaatc aaacatcagc
      901 caatcgctta attggagccg ttcttatgga cctacatgat gaatggattt attcttcaag
      961 aaaatacatc aattttgata agtagaaatg gtaaaaacat tgtatagcat tttacacagg
     1021 agtctggact tgactgagtt tatggaagaa gttttaattg atgataatat ggtttttgat
     1081 attgataatt taaaaggatt tcttaatgat accagttcat ttgggtttat agctaaagaa
     1141 aataataaaa ttataggatt tgcatattgc tatacacttt taagacctga tggaaaaaca
     1201 atgttttatt tacactcaat aggaatgtta cctaactatc aagacaaagg ttatggttca
     1261 aaattattat cttttattaa ggaatattct aaagagattg gttgttctga aatgttttta
     1321 ataactgata aaggtaatcc tagagcttgc catgtatatg aaaaattagg tggtaaaaat
     1381 gattataaag atgaaatagt atatgtatat gattatgaaa aaggtgataa ataaatgaat
     1441 atagttgaaa atgaaatatg tataagaact ttaatagatg atgattttcc tttgatgtta
     1501 aaatggttaa ctgatgaaag agtattagaa ttttatggtg gtagagataa aaaatataca
     1561 ttagaatcat taaaaaaaca ttatacagag ccttgggaag atgaagtttt tagagtaatt
     1621 attgaatata acaatgttcc tattggatat ggacaaatat ataaaatgta tgatgagtta
     1681 tatactgatt atcattatcc aaaaactgat gagatagtct atggtatgga tcaatttata
     1741 ggagagccaa attattggag taaaggaatt ggtacaagat atattaaatt gatttttgaa
     1801 tttttgaaaa aagaaagaaa tgctaatgca gttattttag accctcataa aaataatcca
     1861 agagcaataa gggcatacca aaaatctggt tttagaatta ttgaagattt gccagaacat
     1921 gaattacacg agggcaaaaa agaagattgt tatttaatgg aatatagata tgatgataat
     1981 gccacaaatg ttaaggcaat gaaatattta attgagcatt actttgataa tttcaaagta
     2041 gatagtattg aaataatcgg tagtggttat gatagtgtgg catatttagt taataatgaa
     2101 tacattttta aaacaaaatt tagtactaat aagaaaaaag gttatgcaaa agaaaaagca
     2161 atatataatt ttttaaatac aaatttagaa actaatgtaa aaattcctaa tattgaatat
     2221 tcgtatatta gtgatgaatt atctatacta
//
LOCUS       DACDPS010000178         2222 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.185, whole genome shotgun sequence.
ACCESSION   DACDPS010000178 DACDPS010000000
VERSION     DACDPS010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.185"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(201..457)
                     /locus_tag="IWJ07_002762"
                     /pseudo
     CDS             complement(201..457)
                     /locus_tag="IWJ07_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            complement(800..1823)
                     /locus_tag="IWJ07_002763"
                     /pseudo
     CDS             complement(800..1823)
                     /locus_tag="IWJ07_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
BASE COUNT          717 a          387 c          284 g          834 t
ORIGIN      
        1 atctctcact tcaagagttt taactagatt ccaattatta tttttttgta aaaataaaca
       61 gtctcttaat catagacatt tcttcggtgc cctacttgaa tagcgataac aacgaattga
      121 ttatcttcta tctctaccaa tacacgtatt tgccaatcag agctgcttct gaatttcggg
      181 cgtgaatttt aaacggcagg tcattccagc ccaccaaatt cattaaggat tcttccatga
      241 atactgttgt tttcccactt cttatccaat gtttatgggc aatttcagcc gtttgacgat
      301 tgtattcatc ttctagttgt tccatcgaat agttttttag aagttcagat aaagtaatcc
      361 cataaaattc agccatataa gaaagccatt cttttttggc gtcacttaca cgaaccgtaa
      421 tcatagccct ataattgcct ctcttaaaga atctcatgca tttcctgatg tgattttaga
      481 aaaatttact tgctaaaaaa ctgtgaaaat gtaggagcta gcatccttgt agtaaatgat
      541 cgagctcttt attatacatt aaaaaagttc agaaattttg atgaagtagc caactacttt
      601 ttcatgagta aagcaacact agaaaaagaa tcctccagta cttagaattt actggaggat
      661 gtacttttga agaagcctat aggttattta aaaaatatta ctacaggaag gaacgagaat
      721 tacttcgtta cttataatag aacttagtct tatatagaag gcatagctca cattagctta
      781 tttgtcctat aaaaataaat tacggtattt tagaaaagct attttctctt ttttcaacga
      841 tcttcagcaa tttttcttta tcgtcaggta actcaagttc atgaattcta gcatgacaat
      901 tgggacataa cgcgatacag ttttcaatca catcttttcc accttgcgac aaatattcta
      961 tatgatgaca atgtaaataa ggtacaccgt cttttataaa tggccctttt tcttggcaaa
     1021 gttgacaaat accttttgca agtcgtttaa catattcaga tacgaaggga ttacgtttat
     1081 atatttttgt ttttgagaag caaaactcct cttcgttaac atattttgca gccatttttt
     1141 tattataatt actaactttt cttgcttctt cctctaattt agcattagat aaattacgaa
     1201 tatgtctagt ttgattttct tcctggttat atatctttat cttcaatcaa ttgctcttga
     1261 tttattaaag ctaatggaaa tttatatact tttctgctgt tgcccgaact atctttttca
     1321 ttaacagtat atatttgtcc tgctaaatat acttctccca gatatgtgtg ttcttttggt
     1381 ttaaatgttt caaataaatg aatctttaca aaattacttt ttgaattggc taaaacttta
     1441 ttttgattct tatccaatac ttgatcccct tttagcccca taccggtata ttgtaaaata
     1501 ccatttaacc acttatcttt ataaacatta cttcctgttt gatcactaaa cagtacaaga
     1561 gtattagttt gatgagatct tctcattcca ccctgtttgg aacatttaaa cttctctgaa
     1621 atttctttgt tagtatatac tctaccaact tctaactcac ctaatatgtt tttagaccaa
     1681 ttttctcgcc atgtaataat cttatcaaga ttaaacattt ctccctcttt tggcatgcca
     1741 tttctaatta aattagctaa tacaccagta gaaatatcta atttttttag taattctttc
     1801 ttagtaatca atgttggatt cataacaatg cagctcctaa tgatatcaat ataattgcat
     1861 tagataactt gaaaaatcga aaaaaactca ttttgtgtca agttatctgt aatatataaa
     1921 aatatcatta ttatacctca tctatgtgaa tttttactat tcttacctta gcatattctt
     1981 tataggtata tcagagctaa tcaaactaaa aaatacccga agtcttctta tgtaaaaaca
     2041 ccggttatta cttaaatatg acttaggtaa aacaatgagt gatccaatta taaaatttta
     2101 tggcgaataa tttagtaagc gctacatatt gaatgattta gaaattttga aaaagtagct
     2161 agttcctttt ataccagaaa agtaaaccta ataaaaaaca caactaattt ttagctgtgt
     2221 tt
//
LOCUS       DACDPS010000179         2180 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.186, whole genome shotgun sequence.
ACCESSION   DACDPS010000179 DACDPS010000000
VERSION     DACDPS010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.186"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            700..>2180
                     /gene="ltrA"
                     /locus_tag="IWJ07_002764"
     CDS             700..>2180
                     /gene="ltrA"
                     /locus_tag="IWJ07_002764"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP9334278.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKP"
BASE COUNT          858 a          336 c          455 g          531 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc
//
LOCUS       DACDPS010000180         2170 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.187, whole genome shotgun sequence.
ACCESSION   DACDPS010000180 DACDPS010000000
VERSION     DACDPS010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.187"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..222)
                     /locus_tag="IWJ07_002765"
     CDS             complement(<1..222)
                     /locus_tag="IWJ07_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9334279.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLV"
     gene            complement(641..859)
                     /locus_tag="IWJ07_002766"
                     /pseudo
     CDS             complement(641..859)
                     /locus_tag="IWJ07_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(890..2104)
                     /locus_tag="IWJ07_002767"
     CDS             complement(890..2104)
                     /locus_tag="IWJ07_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAP9334280.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT          670 a          493 c          360 g          647 t
ORIGIN      
        1 aactaaatta tctccttgat actctgttgt ggtggaaaca acaatattca ttctattagg
       61 caacggatga ccgatttctt cataagactt tcgccccata ataaccacat tacccgttgt
      121 taactctcta aattgctttt gttctccctt tattttccat ggtatattac cattcttgcc
      181 tataacatta ttctttgacc tcgcaacaat caaacctatc atctatttca tcctccataa
      241 tatgatgtcg tacctgtgat tccatcaagt gcaccacatc ttaacaaact caaaattatc
      301 tcgaactaac cagctcgaca aattcctttt ttgtcttcat ctgcattata tcatattgtt
      361 ataagcattg ccatttcttt tctgcttttg aaaagttact tatcagccta tgttttgtaa
      421 agggtgatat tcagcatttc tcacgcaaca agcccttgac aaaccataga ctaataagtt
      481 ttaaggtaat caagcagaaa tgaaatagtc tgctgtttca cggcaacaaa aaagagatac
      541 caacacactt atgtagtatc tcttagtcta gtagcaatat tcttttgttt gcaagcaggt
      601 aaacatgggc tgtttcttaa tatctttttg tttgggaaac acctttttcc catcctttca
      661 ccgttgccat ctctgctgcg cacaatgcca cagggataaa ccatagcaaa ccgcctaata
      721 acaagaaaaa tactaagctg aagcctgagg tcgcgaatgt gggatattca tataatgaaa
      781 cgaccattga gatggtcata gccaaaaagc caaagaacgt taattttttt gcaggaattt
      841 tcgttttgct ttgttgcatg aatcttcctc ttttctattt gaatttgttt caattgagtt
      901 tgttgccaaa gaagaaaaag tccaatcctt cttcttgttc ttcttttgta gtagtgatcg
      961 ttgtaaagtg catcgccgat tttaaaatca ctgttgtcag cacataggca tacacggcag
     1021 taatgatgac accaatcact tgaatcccta agaagtacca gctgcttgga ttcgttagta
     1081 aatggctgtt agatgcaaag atccctatca gaatcgtacc tgtgaatccg cccatgccat
     1141 gaacacccca cacatcaagt gcatcatccc attgtttctt tttacgaaaa tcaacagcag
     1201 catgacagac gatacccgca atgatcccaa tcagcatggc actttttgct tctacataac
     1261 cggcacaagg cgtaatggct gccaatccag caacggatcc tgttagtacg tcgacaaaat
     1321 caaagccatt tccttttact tttgcaatga tcagccaagt aatcatcgca aatgcaagag
     1381 cgatgaatgt tgaaccaaat gcattgactg cttgttcatc tgctcgaagt gcaccaccgg
     1441 aattaaaacc gaaccagcca aaccaaagca gtcctgtacc gatagcagct gccataagac
     1501 tgctatgctg acttctttct cctaaaggaa tctttctttg accaagtgtg aggatacatg
     1561 ctaaagagcc gaatccagct gtcgtgtgga tgactgctcc accagcgaaa tctctaaatc
     1621 ccatctgatc caaaaatccg ccaccccata tccagtggca aacagggaaa taaatcagca
     1681 gattccaaaa aataacaagt ataatatagc cttttaaatt caatctccct gcaaaagctc
     1741 ctgtcatcaa aggaacggta atcacgataa acatcagttg gtaaagaaag aacattaaaa
     1801 atggaatcgt tgcaccatga aacatgttgg ggaagcttgt aacatgtctc atcaaaaaga
     1861 aatctgccgg gtttccaata acaccgccga tatcccgtcc aaaggccaat ccgaatccac
     1921 caaataccca cataatcgtt gttatcccaa cggagagaaa agattgagac atgatcgtta
     1981 gtatattatt cttccgtgct aatccgccgt aaaaaaaagc tagaccagga gtcatcaggc
     2041 aaaccattgc tgtacataaa atcatgaatg ctgtatctcc tgtattgata ggtaatgtat
     2101 tcattttttt cgcctccttt ctacgattac gagtataaaa gaaggtgtag ttgttttttt
     2161 cttctgtttt
//
LOCUS       DACDPS010000181         2151 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.188, whole genome shotgun sequence.
ACCESSION   DACDPS010000181 DACDPS010000000
VERSION     DACDPS010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.188"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(101..970)
                     /locus_tag="IWJ07_002768"
     CDS             complement(101..970)
                     /locus_tag="IWJ07_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9334281.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(1022..2014)
                     /locus_tag="IWJ07_002769"
     CDS             complement(1022..2014)
                     /locus_tag="IWJ07_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9334282.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETIEKRTVLPIEFGEGDTTKPLVRNDFA"
BASE COUNT          621 a          484 c          342 g          704 t
ORIGIN      
        1 ctcagattct caagaaagaa gaatgaattg agactattta agtttcagaa taccctttta
       61 aacttccggt atgttatgaa tagttctaat aaatactatt ctaatgattg agtttcttta
      121 ttgctaaagc taggcagcca atcaagcccg cattgtcagc aagccttgga gttagtagat
      181 attcttcgag agtaggcgta tccacgtagg aacccatttt ttctttaaac gattggcgaa
      241 tcaactcgag tagttgcggc tgtttcatta cacccccgcc taaaattatt acttccggtg
      301 catatagtaa cgttgtgtta tacacacact gagcaagata atctgcctca atcgtccaat
      361 aaggatgatc aaatgagata gtctgggctt ttcttccaaa gcgtttttca atcgcaggtc
      421 ctgatgctaa cccttccaaa caatcatgat gaaaaggaca gattccttca aattgatctt
      481 cattatgacg ttgtaagagc atatgtccca tttcaggatg actaaaacct gcgactattt
      541 tcccatccac tacagcacca ccaccaacac ctgtaccaac ggtgtaatac agaacactat
      601 ttttaccttt tccataccct gccatatatt ctccataaca agcagcattt acatcggtag
      661 tccaatagat gggaaccgac aaggcgtttt ccaaagctcc tacaaaatga taattttgcc
      721 aagctaactt cggtgtgttg gtgatatatc cgtaagttct tgattcgatt tgaatatcaa
      781 taggcccaaa tgctcctaca ccaattgatg cgatatttcc tttgtactta tcaaagaaat
      841 caaacacttg ctccatcgtt tcttctggtg ttgtggtagg aaaacttttc cgttctatca
      901 cttgtaattg ttcattacca atcccacaga caaactttgt tccaccggct tctattccac
      961 catataacat tttatctaca cctttcattc actcattttc aaaaaaagaa acgtatttgc
     1021 actaagcaaa atcgtttctc actaaaggtt tcgttgtgtc accttcgcca aattcaatcg
     1081 gcaatacggt acgtttttct attgtctctc cattaatgac agcaattaat tttgccactg
     1141 ccacttccgc catttgcttt gtaggttggg caatcgttga gactagataa ggatctcctt
     1201 ctgtcatctt ttgaccatca aatccgataa cttggacatc ctctggtacg tttttcccta
     1261 ttttttcaag catatcaatg acagctaatg ccattggatc gttgatcgta aaaatcccat
     1321 cgatatcggg attctctaac aaaaaatttg ttacacgttc tgagagatgg tctaaaggtt
     1381 ctaacatatc taacgcttta taggaaatcc ccagttgacg gcactcgatt tcaaaatatt
     1441 ttcgacgact cttcgtttca ttcggtgttt cttggtaccc accgatatat gctacattct
     1501 gacatcctcg gcgataaagt tcatttgcag ccaaacgtcc accggccgca ttgtcagcgg
     1561 tcacataaat cacctcttca gtgaaataac ggtcaatact aacaaaaggc agattactcg
     1621 aaatgtattc atcgatatct gaataagtaa ttccaatgat gccgtctact ttattttggc
     1681 tgaccatctg gatatattct tgttcttttt cactattacc actggagtta cacaaataaa
     1741 gcttgtagtt ttgtctttct aatgctgttt ctacatggta ggcaaattcg ccaaaaaatg
     1801 gatgccatac cgtgggtaga atcaaagcaa ccgtgttcgt atgcttcatc tttaaccccc
     1861 gggcataaaa atctggttga tagtttaact ctgtcattgc tgcttcaact ttttctctag
     1921 tagaagcttt aacagaaccg ttattcaata cccgggaaac agttcctact ccaacacctg
     1981 ctaattttgc gacatctttc attgttgctt tcatagttga tccctcacat ttctgcacaa
     2041 gtttagtata atgaattttg tcataaatta ctaatttttt caaaatacta atagcaaatc
     2101 catgggaaat ctagctatct gttcttttct aaaaacatta tccttcaaaa c
//
LOCUS       DACDPS010000182         2143 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.189, whole genome shotgun sequence.
ACCESSION   DACDPS010000182 DACDPS010000000
VERSION     DACDPS010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.189"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(117..707)
                     /locus_tag="IWJ07_002770"
     CDS             complement(117..707)
                     /locus_tag="IWJ07_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAP9334283.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQKEGWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKKIRLYKLGFYDYYKKYGDPFS"
     gene            1230..1391
                     /locus_tag="IWJ07_002771"
     CDS             1230..1391
                     /locus_tag="IWJ07_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334284.1"
                     /translation="MEPKDKYLDYARVNKDKFIETIIKGKIPDDEKDAVFMAGSPGAG
                     KTEVALGLA"
     gene            1410..1904
                     /locus_tag="IWJ07_002772"
     CDS             1410..1904
                     /locus_tag="IWJ07_002772"
                     /inference="COORDINATES: protein motif:HMM:NF018154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin"
                     /protein_id="HAP9334285.1"
                     /translation="MIDADYFRAQFPEYNGKNSSVFQKASSWLVEQSLKYVLEHGYSF
                     ILEDTFAILSAEKNIIRALKNNFRVTIFYVYQDPKVAWDFTRKRELAEGRHVPKEMFI
                     NAFFKSRENIEKVKNRHPEVVLHIMIKDYQNDISEVHYAADNIQLVLPIQYTSKELEE
                     ELHD"
BASE COUNT          773 a          332 c          364 g          674 t
ORIGIN      
        1 atgaatataa atagagtgta tcttctttga taaatgtcag aaaaagtgtg acaaataacc
       61 atcagtaaaa ttacttgtca tactattgat aaggagtatt taattgaaga gaatgattag
      121 gagaaaggat ctccgtactt tttatagtaa tcatagaatc caagtttgta tagacgaatc
      181 tttttttctc ttatttcttc tgtttcaaaa tggactgctt gaagaatcaa gtctgtgaat
      241 tctataggag ctgttccatt cgcagtaatc aaattacgat ctttcacagc ttgttgttct
      301 ataaaatctt ctttattctg ataattttcc gattctaacc aaggaaattg cgcatttccg
      361 gtgtgtttgt aaccttgcaa taatccgctc tgagctaaat aatcaactgc cccacaaata
      421 gcagctactg gtacgtctgt ttttaacaat ttatcgatta tttgatataa atgatgattt
      481 tttatgtccc atgaattgcc accgatcata ataaaaaggt caactttttc aggtatttgt
      541 tcgattaagt aatcgatttt tgtttttaat cctccaatgg aagtgacctc tgattggata
      601 gaagcagttt tgttttgcca tccctctttt tggtttagtg tgctagtaat ataagcacct
      661 tcccaatcgg catattcctc taatagaaac gtaatcgcag ttttcatttt caagctctcc
      721 tttactatct atgacaagga caacagtttt aacatacaga cattcattaa atttggatag
      781 cgacagcgaa aacaacaatt cttaagtttt agacagagta tacagacagt caacttaatt
      841 aaatacgaac taaataaaaa tgacaggtaa tcatttttgt ttaccagcat tataacattt
      901 tgcatacatg gatgacaaaa gaaccaattc ccacttgctt gagtacaatc ttaaaagaaa
      961 ctggaaggca aaaagtaatt atagaaacga aaaataaatc atatcgggca ctcagacatg
     1021 aaaacaaccc tagaaatata taactagtcc aatgaaaaaa aagcgaatgc cgtagaaaac
     1081 aaggattctg cgtacattca tatctcattt tgcgagcata tctgctatta ctgtgactaa
     1141 atgttaatat ggttaaaatg accgttatat tttttatata aatatcgtat aattagtatg
     1201 aatatagaag aaggaaagaa ggtgcaatct tggaacctaa agacaagtat ttagattacg
     1261 ctagggtcaa taaagataaa tttattgaaa caatcatcaa gggaaaaatc cctgatgatg
     1321 aaaaagatgc ggtattcatg gctggaagcc caggagcagg taaaacagag gttgcactag
     1381 gtttagctta aaattatgac aaccatgttg tgattgatgc ggactatttt agagcacaat
     1441 ttccagaata caatggaaag aactcaagtg tttttcagaa agcttcttct tggttagttg
     1501 aacaatcatt aaaatatgta ctggaacatg gctattcttt catactagag gatacttttg
     1561 ctatccttag tgcagagaaa aatattattc gagcccttaa aaataacttt cgcgttacga
     1621 ttttctatgt ttaccaagat cccaaagttg cctgggattt cactcgaaaa cgtgaactag
     1681 ctgaaggacg tcatgtgcca aaagaaatgt ttatcaatgc gttttttaag tccagagaga
     1741 atattgaaaa agtcaaaaat agacatcctg aagtagtcct tcacataatg attaaggatt
     1801 atcaaaacga catttctgaa gtgcattatg cagcagataa tattcaatta gtactaccaa
     1861 tacaatatac ttcaaaagag ttggaggagg aattacatga ctgagaaaga acaagtacaa
     1921 caaatcgtga agaagtataa taaaagtatc gctgatttat cagaaaacgc ctcagcgaaa
     1981 gaatttaaaa cagtaatgaa ttacgtagca gatgaagcaa atcgtaagca gcgaaaacta
     2041 gttgggttgg ataaataaat tttttaaacc atatccttgt aaagttgagt tttttatttt
     2101 aatgaattaa gactattaaa tagataatta ttgtaactta aaa
//
LOCUS       DACDPS010000183         2104 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.190, whole genome shotgun sequence.
ACCESSION   DACDPS010000183 DACDPS010000000
VERSION     DACDPS010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.190"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            169..1539
                     /locus_tag="IWJ07_002773"
     CDS             169..1539
                     /locus_tag="IWJ07_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HAP9334286.1"
                     /translation="MMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDFFRYYLAAITGE
                     YSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPEGKYSEVLSDFQ
                     SMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNGDDTSRVSKAFP
                     SYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNLLNANAYAMRLA
                     RWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYTGLVNTIGNLTL
                     VTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISARSEALINELITM
                     YPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYSKIKDIASDSLD
                     ETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDYWKYDNGISIND
                     SLRNKK"
     gene            1560..1844
                     /locus_tag="IWJ07_002774"
     CDS             1560..1844
                     /locus_tag="IWJ07_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334287.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
BASE COUNT          731 a          307 c          444 g          622 t
ORIGIN      
        1 ttgatttacc aacatgttta gacattttcc tctaggaaaa aattaactag caccacgcgt
       61 attgtttata ttaaattaga tgaccgtgat aatgcacagc aaatttttga aagtatcaat
      121 tctactggtg aaagattgac tgcctcggat ttgattcgaa acttcatcat gatggataaa
      181 tctaatgaag agcagactac gctttatact aaatattggc gtaggttgga agaagtcttt
      241 gataattcta aaggaatgga agactttttc agatactacc ttgctgcaat aactggggag
      301 tattcagcaa agcatattct ttatcaagca ttcaaagact attggcataa agagcgcgat
      361 gtatcaagtg atgccgaatt gctccaaaaa ttagtgcgat atgctggtta ttttgcgaga
      421 ctttactata acaaacctga aggaaaatac tcagaagtat tgagcgattt ccaaagtatg
      481 gaaagtatga tgcctgcacc ttttgttttg ggattatctg aatggtacta tcatgaccaa
      541 tttatcactg aagaccaata tattgatgcg attaaggtag tgaatgatta tcagcttagg
      601 agatatttta atggggatga tacaagcaga gtgtcaaaag catttccttc atatttgaaa
      661 agtgttagga agtacgctaa agctaatgga tatgaaaata tcgttgatat tgtaatttat
      721 gttcttgtta ctagaaatca atcaaataat atggcacttc catctgacaa ggcattgaaa
      781 tctaatctat tgaatgccaa tgcttatgcg atgagattgg cccgttggtt acttgaaaaa
      841 atagagaatc gagaaaattc agcaacactt gatatgagta acttgtccat tgaacacatc
      901 atgccacaga ctagtacttc atattgggaa gagaaggctg gtacatcggg ggaagaatac
      961 acaggattag taaataccat tggtaattta acactcgtta caaatcctga taattcggcc
     1021 gcgggaaata aagactttga gaccaaaaag aaaatctttg aagataccct tcatattcgt
     1081 atgaataaag atttgtacga attgactgag tggacatcat cggatatttc tgccagaagt
     1141 gaagctctaa tcaatgaact gattactatg tatccctatt tgagatcgtc gggcgactat
     1201 gaacacgatg ggaataggga aattttctta gaggcgcaag gaataaaagc aactgggtat
     1261 ttgaacgaag acgagaccgt gattatacat tcaggttcag aaatttattc taagattaaa
     1321 gatattgcat ctgattcgct agatgaaact cgccaagagc tgttggataa tggaatcatt
     1381 gaagaaacaa ttgggggact acaatttgtt caagattaca ctgcctcatc tgtttcgaat
     1441 gcagcagctc tgctattagg gggtagccgg aatggttggg actactggaa atatgataac
     1501 ggaataagta ttaatgattc gctaagaaat aagaaataga gcatagaggt ggtgttaggt
     1561 tggataagaa caaactaaga tatgcaattc ttaaattgcg tgatgaaaaa aagaatcctt
     1621 ttattgagct taaagggcaa gttccagaag aagatatttt agaacaaact aaatttcttt
     1681 cgcgcaatgg cttactagaa tccatggttt gggcggataa cactgttcat atctggggga
     1741 gtgtttctct ggaaggggaa aaatatttag ttgaaaactc aggtcttgct aaggcgtatg
     1801 cattggcaaa agaagttaaa aattggattc cattttgggg ctaagataag tttttaaacg
     1861 gagaaggtag caaatcataa ttggtttgtt gccttttttg attgcccaaa aagaaaaatg
     1921 acaaagtata gagataaatg acatagtaca cgggaaaaat gacattgtat caccgacaac
     1981 gtctaaatag atgttgatat atatacgttc cgtacaaata attataaaaa tttaaatgat
     2041 ttgagtagaa gtatcagtga atatctcaag gggaatagct atctcaagat tcgattttca
     2101 cata
//
LOCUS       DACDPS010000184         2087 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.191, whole genome shotgun sequence.
ACCESSION   DACDPS010000184 DACDPS010000000
VERSION     DACDPS010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.191"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            48..725
                     /locus_tag="IWJ07_002775"
     CDS             48..725
                     /locus_tag="IWJ07_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP9334288.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            715..1857
                     /locus_tag="IWJ07_002776"
     CDS             715..1857
                     /locus_tag="IWJ07_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAP9334289.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            1870..>2087
                     /locus_tag="IWJ07_002777"
     CDS             1870..>2087
                     /locus_tag="IWJ07_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9334290.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPIT"
BASE COUNT          753 a          272 c          429 g          633 t
ORIGIN      
        1 ataacaaaaa cattgaatca aatattgaat gatggggaag atattaagtg gattcaaaaa
       61 caaatagctt attaaaatat actagtgatc catacattat tattggggct ggcggacacg
      121 caaaagtcat tattgaaacc ttaagattag aaaataagtt aatatatgga ttttttgatg
      181 ataagcaaaa taaaatgcaa gatctgaaat atcttgggaa aataaacgaa ttgaatagct
      241 gggcggatag caaaaaaaaa atctataaat ttattgtagc tattggtgac tgtgtagtaa
      301 gaaaaaagat tataaaaaaa ttgaatatgc caagtgagca atatggaata gctgttcatc
      361 caagtgctat agtggcaaaa gaaatttcaa ttggttatgg aactgttatt tttgcgaatg
      421 ctgtaattaa cccagatgct gtaattggag agcatgctat aataaacaca ggtagcattg
      481 ttgagcatga ttgtagaatt aataactatg ttcatttatc tccaggtgta tgcttagcgg
      541 gtggagtaca tgtaggtgta ggaactcaaa taggaattgg ttctcaatgt atccagatga
      601 agaaaattgg atcttggtgt atgataggcg cagggagcac tattgttaag gatattccct
      661 cacattcctt agcttatggc aatccagcta aaattaagaa agaaggaatt aattttgaat
      721 tctagaattt tattatcaag tcctcatatg ggaggaacag aagaaaaata tgttgctaag
      781 gcttttaaag ataattggat agcgccatta ggtgaaaatg tggataaatt tgaagaaagt
      841 attacaaaat atactggttc taagggcgga gcttctgcgt taagttcagg tacagctgcc
      901 atacatcttg ccttaagact gcttaatgtt ggaaaaaacg atattgtctt tgctccttca
      961 tttactttta tagctacagt aaatcctatt ttatatcaag gggctgtacc agtgttcatt
     1021 gactcagaac ctaatacatg gaatatgagc cctgttgcac tagagagcgc attagaagaa
     1081 gctgaaagaa acaaccaatt acctaaggca atcatcgtag tgaatttata tggtcaaatg
     1141 gctgattatg aacgaatttt agctattgca aaccaattta atgttccagt gatagaagat
     1201 gcagcggaat cgttaggttc tacttataag ggaatttcgt caggcttatt tgggacaatt
     1261 gggatctatt catttaacgg taataagatc atcacaacct caggtggagg agctctagtg
     1321 tctagtgata aaaaatatgt tgagcattca agatttttag caacacaagc aagagatcaa
     1381 gcaccgtatt atcaacatag cgaaataggt tataattacc gtctaagcaa tatttctgcc
     1441 ggtatcggac gtggacaaat ggaagtattg aatgatagag tagctaaacg aagagaaatt
     1501 ttcaatcgat ataaggaagc tttttctgga attgatagaa ttgaaatgat gccagaagtg
     1561 gatggcatgg tgccaaatcg ttggttgtct actttaacga tcaatgatta taattctatt
     1621 gatcaagtag ttaacgtaat aaataaacta aatgaaaaaa acattgaaac aagagctttg
     1681 tggaaaccac tacatttgca accagtattt caaggggtaa aaatgtatta ccatgataat
     1741 aatcttagtg tgtcagaaaa gttatttaat agcggcttat gcttaccttc tggaagtaat
     1801 atgacagata gtgaacaaga atttgttatt gatgagttta aaaaaatatt caagtaggtg
     1861 tgatatttta tgaacatagg aattattggt ttaggttcca tggggaaaag aagacttaga
     1921 ctcattcaag aacactttaa tgatatttta ctatgtggtg ttgatactaa tgaggcgaga
     1981 cgaaaggaag ttgaaaagga atttggaata ccaacttata atagtctaac agaggcaaaa
     2041 aatttgtttg aaataaattc agtttttgtt tgtacatccc ctataac
//
LOCUS       DACDPS010000185         2020 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.193, whole genome shotgun sequence.
ACCESSION   DACDPS010000185 DACDPS010000000
VERSION     DACDPS010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.193"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            100..570
                     /locus_tag="IWJ07_002778"
     CDS             100..570
                     /locus_tag="IWJ07_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAP9334291.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            792..1895
                     /locus_tag="IWJ07_002779"
     CDS             792..1895
                     /locus_tag="IWJ07_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP9334292.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT          686 a          328 c          460 g          546 t
ORIGIN      
        1 gaatctctga agaataaatc acgtgatcgt gacgataaaa tacacttacg tcaggtgata
       61 aaaaatacat aagtagaaaa ctaagaggag gaacagaaaa tgaaagaagt tcaattgatc
      121 agaaaaagcg aactatcaga aggtggttgc aatgcatgcg gcgtagtaga agcaacaagc
      181 tatacattaa agcttggtgc caacaaagcg ataatctctg agttgacagt aggcggtctt
      241 gtggacagtc tggcgcttgc tgaaggtttt attggagaag atatttatga gatgttttca
      301 gaaatccgtc agttaaaaaa aggggaaaac tgcatagagg ttcatcatga gagcccgaat
      361 gtgagattca aacgtggtga taatgagatg atcttcaata atcatgtgtc cgatcacacc
      421 gagctatatg gaatcgtgaa tcaaatacta actgagcttt ttggattggg accttatgcg
      481 ttcaaggaag aaaacggtaa tccaaaatta aacgaagaat ggcaagaaac gattgaaata
      541 caaagaaaca acccgcactt atttcaatag gcatccacag tatttcgtaa aagggacttt
      601 gacacgactt ttgtcaaaat cccatcgttt tttatctatt tgtgttctaa acatttcttt
      661 cagagtagta acaactggaa tgactgaaat gtaaacagat tatttgcctg tatcaatacg
      721 gaagagtaat ataaagtgtg taaacgctat catattattc attcaatttt ttatagaggg
      781 aaggcattga aatggaaaaa acgtatcaag caggtacaaa tgaaggaata gtggatttta
      841 tcaatatgga agatttagaa atagccgctt cacaagtgat tccagctggt ggttatggtt
      901 acatcagcag tggtgctggc gacttattta cttatcaaga aaatgaacgg gcattcaatc
      961 atcagctgat tattcctcat gtattaagag atgtagaatt acccgataca actacacatt
     1021 ttgacaaaga aacgctgact gcaccaatca tcatggcacc agttgctgct catggcttag
     1081 ctcatgtgaa ggcggagaaa gcttctgcaa aaggtgtagc agattttggg acgatctata
     1141 cagcaagttc ttacgcttct tgtacattag aagagataag agaagcaggc ggggagaaag
     1201 caccgcaatg gtttcaattt tatatgagca aagatgatgg gatcaatttg gatattcttg
     1261 aagtggccaa gcgtaatgga gcaaaagcaa tcgtattgac agcggatgca acagttggcg
     1321 gaaaccgtga aacagaccgc agaaatgggt ttaccttccc actgccaatg ccgatcgttc
     1381 aggcttatca atcaggagta ggccaaacga tggatgctgt gtacaaatct tccaaacaga
     1441 agcttagtcc aaaagatgta gaatttattg ctgctcattc tgatttgcca gtctatgtga
     1501 aaggtgtcca gtcagaagaa gatgtctatc gttcattaga atctggtgct ggcggaatct
     1561 gggtatctaa tcatggtggc cgtcaactag atggtggtcc agcagcgttt gattcgttgc
     1621 aatatgtggc agaagcagta gataaacgtg taccaatcgt gtttgacagc ggtgtacgac
     1681 gcggacagca tgttttcaaa gcaatcgcat caggtgcaga tcttgttgct attggcagac
     1741 ccgttattta tggattgtct ttaggaggaa gtacaggagt ccatcaagtg tttgatttct
     1801 ttaaaacaga attggaaatg gtcatgcaat tagctggaac acaaacagta gaagatatca
     1861 aaaaaataaa attgcgtgag aatcgtttta tttaagaaac aaatgacgaa aaaaatagga
     1921 gtgcggcaga tgatgccgta ctcctatttt tatagttcaa ggatgtcact aatagaataa
     1981 tttgaaacga aaaaataaaa ttgaaatatt ttttcatact
//
LOCUS       DACDPS010000186         2014 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.194, whole genome shotgun sequence.
ACCESSION   DACDPS010000186 DACDPS010000000
VERSION     DACDPS010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.194"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            244..861
                     /locus_tag="IWJ07_002780"
     CDS             244..861
                     /locus_tag="IWJ07_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9334293.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            902..1201
                     /locus_tag="IWJ07_002781"
     CDS             902..1201
                     /locus_tag="IWJ07_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334294.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1235..1402
                     /locus_tag="IWJ07_002782"
     CDS             1235..1402
                     /locus_tag="IWJ07_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9334295.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(1447..2010)
                     /locus_tag="IWJ07_002783"
     CDS             complement(1447..2010)
                     /locus_tag="IWJ07_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9334296.1"
                     /translation="MIFKLRNRTEGLNIDKLFQEKASGKDTERPEFQKLLNYIREGDC
                     VIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLDTAMFDMFLSL
                     LSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLVFKTVVDMLKQ
                     GIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT          653 a          332 c          394 g          635 t
ORIGIN      
        1 aaactggata actagtttat ttaatggcag accgttaagg cctgccattt ttatgtattt
       61 tagcggaata aatataatca tgcgggagtg taaacttact gtttcaaaaa gtatactttt
      121 tgacatgcct tgattttctt tgtttatgta tggcataata accacataat acaatttgaa
      181 acgtttctga tttaatctga ttgttttgaa acaattttag tgaaaaaagg gtagataaaa
      241 acgatgagaa aattttttta tatccgggtc agtagttatg atcaatcgat cgaacgacaa
      301 ctgatttccg cgaaggaatt aggaatccct gaagaatata tatttgtaga aaaagccagc
      361 ggcaaagatt ttaaacgccc tgaatatcaa ttaatgaaac ggatgtttcg aaaaggagat
      421 ttactctatc tacaatcatt agatagacta ggcagaaata aacaaatgat tttagaagaa
      481 tggcgagaat taactaagga aaagcagatt gatatttctg tgttagacat gcctctgtta
      541 gatactgcta gttacaagca actagatggg ttagagacgc ttatctctga cttaattctg
      601 caattactca gctatatggc cgaagacgag cggaaacgaa ttaatagccg gcaaagagaa
      661 ggcgtagaag ctgcgaaaca aaagggtgta aaattcggca gaaagaaaat tgctttacct
      721 cctgaatttc ctgcgatcta ttctgactgg aaagcaggaa agataactgc agtctatgct
      781 atggatcaat tgggcatgaa acgaaatacc ttttatcgac gggtgaaaga atatgaacat
      841 accttgcacc acttttcttg atagatataa cagctattag acgggaaggg agagtttgaa
      901 aatggactta acgaatcaaa gggtcgtttt tactggaacg ctgcagcaaa tgacacgaac
      961 ccaagccatc ggtttggttc actctttaaa cggccattgt caatcaagtg ttacttcaaa
     1021 aacaaatttc ctggtttgtg gtcagtattc taagtcgttg tttgatgatt ctctctatac
     1081 aaaaaaggaa caaaccgcgc gggacttgat tgcgttaggt catgagatta ccattttatc
     1141 agaagtcgaa ttttatgcgt taattagtca acagttgaaa gaatggcaac ggtatttata
     1201 atcgttagta aaatttacaa ggaggaaaat ttttatgaag cgaaaatctt atgatcgttg
     1261 gggcattgaa ataactagtt ttaatcgaaa tattgctttt cacgtggagt gtgactgtgg
     1321 ggaattagca aaaagtgttt atcaaaaaaa ttacttttac tgtcctaaat gccatcgccg
     1381 atacggggta catggaacat agaaacgaaa tagtgcggta gaaaagcgaa tgaatgtagt
     1441 atatttctac aaatcattcg ctttctttat tttataaata gttggtcgag agatcccgtt
     1501 ttcttttgca atctctgcaa ctggaatgcc ttgctttaac atgtccacca cggttttaaa
     1561 aactaagcgt ttttgcggat ctgttgagtt aatagaatac tcgattggtc gtcccttata
     1621 tcggccggct ttttgagcca ataatactcc ttgccgctga cgttcacgaa ttttctcacg
     1681 ctcgttttgt gcaatataac ttagcagtga taaaaacata tcgaacatag cggtatccag
     1741 gagttcattt ccagtattaa aatctaaaaa ctgtgcatct aatattttta acgctacttt
     1801 tttctgtttc ataaaagcaa ctgtattttt tatatcttca taatctcttc ctaaacgatc
     1861 tagtgatgta acgatgacgc aatcaccttc acggatataa tttagtaatt tttgaaactc
     1921 aggacgctca gtatctttac cactggcttt ttcttggaat aatttatcaa tgttaagtcc
     1981 ttcggttctg ttgcgaagtt tgaaaatcaa acgc
//
LOCUS       DACDPS010000187         2012 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.195, whole genome shotgun sequence.
ACCESSION   DACDPS010000187 DACDPS010000000
VERSION     DACDPS010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.195"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            627..959
                     /locus_tag="IWJ07_002784"
     CDS             627..959
                     /locus_tag="IWJ07_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP9334297.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1578..1919
                     /locus_tag="IWJ07_002785"
     CDS             1578..1919
                     /locus_tag="IWJ07_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP9334298.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT          698 a          271 c          427 g          616 t
ORIGIN      
        1 gaaaagggaa cgtatgtttg catactcctt ttttttgagt tataataata cgcgaagaga
       61 gataatttca ttatttaaac aagctaaaaa tcttgatatt acttcttttc aggcaatacg
      121 aatattattc gcttacattt tttgtataaa tttaattgtc tgctatgctt gtgatgaatt
      181 gattttcgtt ctgcactttt ggatgataaa tttggacaat tgaaaataca aaaaaaagaa
      241 aggcggtatg aactcggaag gctggtaacc ttcgtcagcg ttcatacacg gcattaagat
      301 ttggccctta atgcgttaac ctttcagtgt tacggtatta cccgcaatgg atttagtata
      361 acagatattc aaaagtgata ccactttttt tggctgtgtt aaggttaaaa atacgtgata
      421 ttacgatgaa tctatgccta agcacaatgt tataaaaatg tccgtgtggc tctttgccgt
      481 atgacgaacg ctgaaattag agggaacgca tgtttggatg cgttccctct tttttgtttg
      541 tcatgaacga aaatcaattc tctatttttg aaaaataaca agaaaaactt gttaaaaata
      601 tttaaagaaa ttgggtggtt tattttatgt catatacagg aattttgagt ctcaaagata
      661 tttgtcacta cggtaaacgg tgtaccgcta cagagaaaat cacgaagaaa ctatcaactg
      721 gccaaagtaa aaccgttgtg cagtgtaaga aatatataat ccagaaagac aaagtctcag
      781 aggaaatgat ttattacatt gggaaacaaa agcaaataat tttaaaagat cctatcccct
      841 taaaagagct atatccaaca atcaaacatg tttatgacca aaacggtgta ctgattggtc
      901 gaagaaaaaa tggagtcttg cgttgtaccg caaaagggat ggggcgtttg atcggctgaa
      961 ttgatcattc tcataacttg tattttatcg tttttcgctt gcttgaaaat attgtcgaga
     1021 cttctaaagg aacttagagt cactagaaaa ataaggagta gacttgatct atcaacaatt
     1081 cccttcgggg agtgacgggg taggttttaa cgattggtaa cgtaaaaaat tggtcaatcg
     1141 agtaaatgcg cttgtttgga tagtggtgaa atgaatttgt gtaaataata agcgcagagt
     1201 aaacaagagg gaatttagaa gttagacaag ttagaatagc gtctagatta atcatgagca
     1261 gctactaagg gatctagact ttgacgcaaa ctcatggaat aaggagtagg tcttctcgta
     1321 atggggagga cgcacaagaa attgtgtcgt gtgctgcagt acatgtagat acttgcgagg
     1381 acgttgatta ttggatcaat cgagcattta ccgctgtcta ctagggtttc tcgtttcgga
     1441 cgccataaac gagtcgaaat tttttatgta tttttaagta aacgaaaaac gataaaatat
     1501 gaacaagtcc tgaataaagt gaaaaaattg atataaaaca tagagaaaat gaaatttacg
     1561 taatggagga atgacaaatg aaaaattatg atccgaatat acgattgggc acacatacta
     1621 ttaaggtttc tttccaacgt tgggattaca aaggatttgt gacgtttaga agaggcggaa
     1681 attgtaaagg attagatgtt ttagcattag acgaggatga tttatacgat caaacattga
     1741 ctgataatcc aattggattc ggcttgctac ctgaagatga tgaaggtaat gaatggttta
     1801 aaatgacgct tatgaatgat aatggagacg aattatctgt tgaagatact tggtcatatt
     1861 tgagtgatta tattgtcagc tttgaaatca ttgaatttgt agcggataaa gaagaataaa
     1921 aactggagga ataaatttta cagataaagg cttagaagga gtcacgatga ttaataaaaa
     1981 ggtagtatta ccttaggcat tgagtaagga at
//
LOCUS       DACDPS010000188         2010 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.196, whole genome shotgun sequence.
ACCESSION   DACDPS010000188 DACDPS010000000
VERSION     DACDPS010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.196"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            34..1368
                     /locus_tag="IWJ07_002786"
     CDS             34..1368
                     /locus_tag="IWJ07_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334299.1"
                     /translation="MDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAY
                     GLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVV
                     NLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITT
                     YTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQF
                     PWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQS
                     KHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAAD
                     NNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEIT
                     TKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
BASE COUNT          711 a          270 c          345 g          684 t
ORIGIN      
        1 agtttttatt atggagaaaa gttttttaaa aagatagatg ctgctttttt atttagttgt
       61 ttatatactt ttgcaactta tcgaacagtt gatatctatt atcgatccgc aattgcagaa
      121 gcaatcgctc ttacgattat cccagctgtt ttatattatg cttatggatt gatattcaat
      181 agagaaaaaa aagtcatcgc tttatctgtc agtataagtc tacttgtgta tactcatgtc
      241 ttaactacac tattgtgtag tgcattgatt gcttgtatga ttatagttcg tttgttttta
      301 agcaagcaaa taaaaaacga tataaaattt gtggtcgtga atttagtaaa ggctgttttt
      361 ttgacttgtg cattaactag ctatttttgg tatcctatgt tgcagcagat gaattatcaa
      421 gaaatacaca gtcccttcaa aacagatttg cagctagaag cactttctat ttcagatagt
      481 atcattcaag cgttaaataa cgatattaca acatacacta tggggttatt aggattggtt
      541 gcgttgattt ttcctttatt catactgaaa gaaatggaga aaaaagaaaa aatcatctat
      601 gttggcgtag tttctacatg ggtatgcgta actaatttat ttccatggtt tttgtttcaa
      661 aaaacaccga taagcttact ccagtttcct tggcgtatct taggtatcca aatctttttt
      721 tcttcaatga tcataacgct tgtttttatg aaaaagacta tgaataaaaa atattcatgg
      781 ttaatcattg gcgctactgt ggtgtttcta ctcgtcacgt ctatagcagc aaaagaaaat
      841 tacgcaaaag ttatccaatc aaaacacggt catattgtta tcaatgaaga aacactccca
      901 cgttatacaa catcagatat gggaggatta tacgattacg ccccaacaga agcgataaaa
      961 gaaaggcctc acttagaaaa acatgaagta aaagtaggag aatcgtggga aaaagggaag
     1021 tataaagctg cagataataa cattacttac acagtggcaa gcaacaaaaa gcaaaaagtg
     1081 acagtaccag tatatgctta tttaggtaca caggtaaaag taaacggtaa agtcgtaaat
     1141 gactcttata aaaaacatgg cttagtgaat gtggatgtaa atgctggaga aaataagata
     1201 gaaattacaa caaaatatac tccaactgct ctcatggctt tatggatcag tattctgact
     1261 tattgtttag tggtgtattc aagttttaaa cgatttgcac ctaataagtt gaaagattct
     1321 cgatatgtga ctaaaaagga gataaacaag aagaatgaaa ataaataaaa aagtagtaat
     1381 attgagtgga ttaattatag ttagtagtat atattctttc atctttttca aaaatacaca
     1441 gagtatttat accactggag atctttcttt tcacttatct cgaatcaagg ggttatcgac
     1501 tatttttacc agtccgatta attatgagac attcaattat actggatatg gtgtaaatta
     1561 tttttatccc tttctgactt tttttccagc tgtaatgtta tattggatga caaaaaattt
     1621 gattgtaagc tatattattt acgtttggtt attgaacttg tgtacaacac tcgtagctta
     1681 tcattatggc gagcggtttt taaaacaaaa aaaagcggca tttttatttt aagtagttca
     1741 gttttggagt acaaaatgac aaaatgtact tcaaaatgga ctactttttt cattttaact
     1801 tacataccaa attacataca aaatgattta taaaatgcaa ttgaaaatga taattgaatg
     1861 aaaatttgtg attttgtggt attatgataa tataacttat agaaaaaatt gcttattcaa
     1921 ctaacgggca ggataatgag aaaaggtttt ttgatagaaa taagaaatta gatggtaaat
     1981 tagttaaaaa ggctgtgtta taaatgaaag
//
LOCUS       DACDPS010000189         1991 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.197, whole genome shotgun sequence.
ACCESSION   DACDPS010000189 DACDPS010000000
VERSION     DACDPS010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.197"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(631..921)
                     /locus_tag="IWJ07_002787"
     CDS             complement(631..921)
                     /locus_tag="IWJ07_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334300.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1129..1821)
                     /locus_tag="IWJ07_002788"
     CDS             complement(1129..1821)
                     /locus_tag="IWJ07_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP9334301.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          551 a          377 c          300 g          763 t
ORIGIN      
        1 aatacttaat tgttaaatac cttatttgat gcaaaagaaa tctacgatag tcaacaaaaa
       61 aattttcatt atatccagaa aaatttttct tagttaagga cctatggatt gcagtaatgc
      121 agaattcgtc gaataaactg ccaaagacat acaatcatat tgcttttaaa attgacgaac
      181 aagatataga ttcgtttgtc tcaaaaatcc aaatgttagg tttaactgtc gaaccaggac
      241 gttctcgtgt gaaaggcgag gcttcttcaa tttactttta cgactatgat aatcacctct
      301 tcgagttaca taccggtact ctccaggaac gtttaaaatc ctacaattcc actacctgag
      361 actagtaatg actagtctct ttttatggcc tctgatcccc ccattgagcg aatactaata
      421 gggcttcgaa ttgatctgtt caatctttaa gcaatatccc tcaaatgagt ttggaaagtt
      481 tagtgattca tcacaaatac ttaaatacat gtagttatat atagattgag gttcttgtga
      541 gcgtatgctc gtgtgggttc aaatcccatc gtctgcatcc atctttccaa aacaagcggc
      601 taggacattt ttgtctcagc cgcttgtttt ctagtttatt ctttcatctt gtcggacctt
      661 ttttcttggg tatagacttt tctcatattc taccacaatt ttgggtttcc ctaacacatg
      721 ataggatctc acaacaaatt gctttctatt attataataa cctgtaacga ctaacttcat
      781 cccttcgtct acttcagcta ggaagttcaa actatgggaa gcaatcaaac aattgacccc
      841 atttaaactg aaacggatca acggtctggc actaacttgc aaagtacgga tcttatctac
      901 tgttccatat tttttctcca taaccttcac ctcaatacca ttatacgaac atacattccc
      961 tttgtaaaga agaaactctg ttttatcttt ttgacttttt gtctattacc tttcttaaaa
     1021 tcatattggc acttcctgcg aagatgaagg acatgtacta aaaaaaagag gcagcagtat
     1081 agattactgt tgcttcttta ctaatttagg tttacttgtt aagaaagatt atttacgcaa
     1141 atgttcttca acaatttgct gagatttatc agctgtatct tccacttcat cttttgcttt
     1201 ttctaacttt tgaatatatt gtttcaagct tgagttttca gcagttgctt cgtctaattt
     1261 cttttgtgta tctgttaact gagtttctac atttgttttt tcttgtgtca gtgttgcgat
     1321 ttgttgattc aaaccattta cagtattttg taattctgaa agttttgctt gtgcttcttg
     1381 tagtcttgct gtccaatctg cattcagatg attgatttca tcattttttt ggttgatctc
     1441 atctagtttg acattgattt cattttgttt gctttgtagt tgtgcatcct tgctgactaa
     1501 ttgactttct aagttttgtt tttctgctgc cgattgattt ttttggtttt ctaattcatt
     1561 tacttttgtt tggtattctt ggacatctgc ctttgaatct tgcaatgtct gttctttttg
     1621 tgctagtcga gcggtaagat ctgtaagcgc ttgctcttta tctgtcaatg tatgatcaag
     1681 ctgtgtcaga ttctcattga ttgtatcaat atctgtctct ccaccccaca aaataatttc
     1741 atttgcgtac cgcgtgcctg tgtagccaat tagacataga aatgcaacga caatcccacc
     1801 agtaataaat ttctttttca tttgcatagc tccttaatcc aatactttta catgctaaaa
     1861 gtaagtgatc tcaaacacaa tgtcaagcgt ttttaaatta catgtgtaat tttaatataa
     1921 atactatcaa atactcattt aatcctcctt ttttatctat ttaaaaaatc ttttttatag
     1981 agaaaaatgc a
//
LOCUS       DACDPS010000190         1956 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.198, whole genome shotgun sequence.
ACCESSION   DACDPS010000190 DACDPS010000000
VERSION     DACDPS010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.198"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            101..520
                     /gene="ssb"
                     /locus_tag="IWJ07_002789"
     CDS             101..520
                     /gene="ssb"
                     /locus_tag="IWJ07_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP9334302.1"
                     /translation="MMNSVNLVGRLTKEVDLKYTPKGNATGTFILAVNRNYTNANGER
                     EADYIRCVIWRKAAETLAKYTGKGSLIGINGRLQTRSYQNQQGQTMYVTEVLVTDFYL
                     LESKEINEQRSKSMANNQNNSSIPGNEIQISDSYLPF"
     gene            597..893
                     /locus_tag="IWJ07_002790"
     CDS             597..893
                     /locus_tag="IWJ07_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334303.1"
                     /translation="MKIIDSYDQRTMQYLGSFDYTDENIINYVASLPPYQSVRLVDFA
                     SDELVLTTMGNFLDYVGDKKWLEKILSKLIAKQTGEEPINAIQLFNRYEQENND"
     gene            908..1339
                     /locus_tag="IWJ07_002791"
     CDS             908..1339
                     /locus_tag="IWJ07_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334304.1"
                     /translation="MKRLKRSIFIVLALIDFVGTGCEAIDVQKAPLSEIKQAITKQDE
                     EQPLNTQVEIVNQNKPSFSEEDFSLENGTRQFFSDLDRLNRVGVASALLHKSRMPTEE
                     RDRLYIKPTGWKQKKMKNGDYLYNRCHLIGYQRATSFPISM"
     gene            1521..1826
                     /locus_tag="IWJ07_002792"
     CDS             1521..1826
                     /locus_tag="IWJ07_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334305.1"
                     /translation="MKLDMPGRYKLLRVRLFLIKLTNLRMYGSITDLYSKDIRLRMQR
                     TLSKSKNVGHEIRDGRLAVYLSRVGKTCLIDSDESGNATKRFTEEIEQLAELNIRYF"
BASE COUNT          743 a          267 c          398 g          548 t
ORIGIN      
        1 tcatagatat aaaaatcaag ctgtttatga aagtagttat tattaagggg atagtttccc
       61 ctttcaaaaa taataaatag aagaatggag agaaaaaatc atgatgaatt cagtgaattt
      121 agtaggacgg ttaacaaaag aagtggattt aaaatacaca ccaaaaggca atgcgacagg
      181 cacatttatt ttagctgtta atcgaaatta tacgaatgca aacggcgagc gtgaagcaga
      241 ttatatccgc tgtgtcattt ggcggaaagc tgcggaaact ttagcaaaat atactggaaa
      301 aggaagtttg attggcatta atggacgtct tcaaacgagg agctatcaga atcagcaagg
      361 acagaccatg tatgttacgg aagttctagt gacagatttc tatttgttag agagtaaaga
      421 aatcaatgag caacggtcaa aatcaatggc gaataatcaa aataattctt caattccagg
      481 aaatgagatt caaatttcag atagttattt gcccttttaa gtgaaaaata attttgttct
      541 atacctgatt tcttcaggta tagaacaaaa aataaataga gatgaggcga ttttttatga
      601 aaatcattga cagttacgat caacgaacca tgcaatattt gggctcgttt gattatacag
      661 atgaaaatat tattaattat gttgcaagtt tacctcctta tcaaagcgtt cgcttggtag
      721 attttgcaag tgatgaattg gtattaacaa cgatgggcaa ctttttagat tatgtcggag
      781 acaaaaaatg gttggaaaaa attttgtcta aattgattgc gaaacaaaca ggagaagaac
      841 ccataaatgc gattcaattg ttcaatcgat atgaacaaga aaacaatgat taaagaggag
      901 agaaaaaatg aaaagattaa aacgaagtat ttttattgtg ctagcattga ttgattttgt
      961 tgggacaggt tgtgaagcaa ttgacgttca aaaagctccc ttatcggaaa taaaacaagc
     1021 aataacaaaa caagatgaag aacaaccgct aaatacacaa gtagaaatag tgaatcaaaa
     1081 taaaccgagt ttttctgaag aagatttttc gttagaaaat ggaaccaggc aatttttttc
     1141 agatttggat cgtttaaatc gggtaggtgt agcaagtgca cttttgcata aaagcaggat
     1201 gccaacagaa gaacgagacc gattatacat taagccaact ggctggaaac aaaagaaaat
     1261 gaagaatggc gattatcttt ataatcgttg tcatttgatt ggctatcaac gtgcgacaag
     1321 tttccccata agtatgtaat acgaaacggg aggattaatc ttaagattaa tcacaactaa
     1381 cctatttgac agttggaatg aaagctatca tgtctgctga atcaactggt ctgatactcc
     1441 tacatgcaat aaagtctact aggaagttat tgtatgaagc taggtaaagg cggcagaaca
     1501 atttctaagt gtggaatcat atgaagttgg atatgccggg acgctataaa ttactcaggg
     1561 tgagactgtt tttaattaaa ctgacgaact tgcgaatgta cgggtctata accgacttat
     1621 attcgaaaga tataagattg cgaatgcaaa gaacattatc gaaaagtaag aatgttggtc
     1681 atgaaattcg agatggaaga ctagcggtgt atctgtcaag agttggtaaa acatgtttga
     1741 ttgattcaga tgagagtggg aatgcgacaa aacgttttac agaagaaata gagcaactag
     1801 cagaactgaa tatacgatat ttttaaatac tttagcggag aataaaaaaa tcctctacag
     1861 aaaactacgg agggcaacat gtgctattgt ttcaacaata catttttaat ttttaccata
     1921 acaaaaaagt acttgaaaaa aaacagaaat atgtta
//
LOCUS       DACDPS010000191         1945 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.199, whole genome shotgun sequence.
ACCESSION   DACDPS010000191 DACDPS010000000
VERSION     DACDPS010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.199"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            151..1179
                     /locus_tag="IWJ07_002793"
     CDS             151..1179
                     /locus_tag="IWJ07_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAP9334306.1"
                     /translation="MNSKKLQMGEIVRGPSQISIDVTYKCNFRCKHCYNASGGDNCFM
                     DNELTDDELLELTKDICNLKPYNICFCGGETLLRKKIVLSCATLLKEAGIPNIAIVTN
                     GSLVTQDVAKELFKAGINRFQVSLDGKNASSHEALRLYPNSFEKAINAIKLFRELSEE
                     VDVAFCPTSFNYNQLREVYQLVNTLKVKSLRIQPFMLSGRGMQNREIVPTVSQYRKLV
                     SDINYIKRNEDYTKIDWGDPIDHIIRHRTLLKDFSNFVSIRSDGGLMASPYLPIQVGN
                     IKCHSLSKYWRAGLYKVFSLDLVQDIATQVSSIQDMSKELKKGHIKWLNEDIEIDILD
                     EYKHDILI"
     gene            1235..1453
                     /locus_tag="IWJ07_002794"
     CDS             1235..1453
                     /locus_tag="IWJ07_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334307.1"
                     /translation="MISEPQMNNMYESPTIMGAGEAAPRACSAIMVCVLLASAGVGIQ
                     AGAVYTYAVVATVAVAAAAVWKTVGLVG"
     gene            1524..>1945
                     /locus_tag="IWJ07_002795"
     CDS             1524..>1945
                     /locus_tag="IWJ07_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334308.1"
                     /translation="MKYNNLKPKLLNENIEYIREENYGYLTIVPKNGTELKDNVFNPT
                     VRYILANCTGKNSVKKIANGLKKKFPSLEYEKFYQDVVGTIKFFKHYNIIDIGDYSME
                     EKKIYKIEQNLELELLNESHYEEIYNFITDHPESMYFH"
BASE COUNT          752 a          215 c          352 g          626 t
ORIGIN      
        1 tagataatga aactcgcgat aatttgaggg attatatact aaatacaaaa aaagaagttt
       61 gttatataat tataactcat gatcacattc tagataattt tttggattca aatgatgaaa
      121 tattatattt aaataaggag tatgataata atgaatagca aaaaacttca gatgggtgaa
      181 attgttagag gtcctagtca aatatctatt gatgttacat ataaatgtaa ttttagatgt
      241 aaacattgtt acaatgcgag tggtggagac aattgtttta tggataatga gttgactgat
      301 gatgaactgt tagaacttac aaaagatata tgtaatttaa agccatataa tatttgtttc
      361 tgtggaggag aaactttact gagaaaaaaa atagttttat cttgtgccac tttattaaaa
      421 gaggcaggta ttcctaatat tgctattgtt actaatggat cattagtaac tcaggatgtt
      481 gcaaaagaat tattcaaagc aggtattaat cgatttcaag taagtttaga tggaaaaaat
      541 gctagtagcc atgaagcact tagattatat ccaaacagtt ttgaaaaggc aattaatgct
      601 atcaaattat ttagagaatt gtccgaagaa gtggatgttg ctttttgtcc tactagtttt
      661 aattacaatc aattacgaga agtatatcaa ctagtaaata ctcttaaagt gaagtcttta
      721 agaattcaac catttatgct tagtggtagg ggaatgcaaa atagggaaat agtgccaact
      781 gtttctcaat atagaaaact agtttctgat attaactata ttaaaagaaa tgaagattat
      841 accaagatag attggggaga tccaattgat catatcatcc gacacagaac tttattgaaa
      901 gatttttcga attttgtttc tattaggtca gacggaggat taatggcatc accttacttg
      961 ccaattcaag ttggaaatat taaatgtcac agtctttcta aatattggcg agcaggactt
     1021 tataaagttt tttctttaga tttagttcaa gatatagcta ctcaagtttc ttcaatacaa
     1081 gatatgagta aagaattaaa aaaaggacat attaaatggc ttaatgaaga tatagaaatt
     1141 gatattctcg atgaatacaa acatgatatt ttaatttagg tttatcattg gaaagtttag
     1201 ataaacaaag tacttttaga aaggagggaa aaaaatgatt tctgaacctc aaatgaataa
     1261 tatgtatgaa agcccaacta taatgggagc tggtgaagct gcgccaagag cttgttcagc
     1321 aattatggtg tgtgtgttat tagcatctgc aggagtaggt atccaagcag gagcggtgta
     1381 tacttatgct gtagttgcta cagtagctgt agctgcagcc gcagtttgga aaacagtagg
     1441 attagttggt taggaattaa taggagacta actgtctcct attgattcca atgattaagc
     1501 taaggaggaa ttttaagaaa ataatgaaat acaataattt aaaaccgaaa ttacttaatg
     1561 agaacataga atatataaga gaagaaaatt atggatattt aaccatagtt cccaaaaatg
     1621 gaactgaatt gaaagataac gttttcaatc caacagtaag atatatattg gctaactgta
     1681 ctggaaaaaa cagtgtaaaa aaaatagcga atggtttaaa aaagaagttt cctagtttag
     1741 aatatgaaaa attctatcaa gatgttgtag gaactattaa atttttcaaa cactataata
     1801 ttattgatat aggagattat agtatggaag aaaagaaaat atataaaatt gaacaaaatt
     1861 tagaattaga attactaaat gaatcacatt atgaagaaat ttataatttt atcacagatc
     1921 atcctgaaag tatgtatttt catga
//
LOCUS       DACDPS010000192         1882 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.200, whole genome shotgun sequence.
ACCESSION   DACDPS010000192 DACDPS010000000
VERSION     DACDPS010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.200"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..91
                     /locus_tag="IWJ07_002796"
     CDS             <1..91
                     /locus_tag="IWJ07_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP9334309.1"
                     /translation="YFFENVVTGKENWNDLYHANEVLKIAEEK"
     gene            92..844
                     /locus_tag="IWJ07_002797"
     CDS             92..844
                     /locus_tag="IWJ07_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAP9334310.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            849..1796
                     /locus_tag="IWJ07_002798"
     CDS             849..1796
                     /locus_tag="IWJ07_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP9334311.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          652 a          258 c          374 g          598 t
ORIGIN      
        1 atatttcttt gaaaacgtag taacaggcaa agaaaactgg aatgatttat atcatgcaaa
       61 cgaagtattg aaaatagctg aggagaaata aatggaaatt ttatttacta tttgtggtag
      121 agctgggtct aaggggttta aaaataaaaa tttgaaatat ttggcaggaa agcccctagt
      181 ccatttcgct ttagcaatga tagatatcta tcaacaaaaa catcctgaac ataattgcac
      241 gacttgtttg aatacggaca gtaatgagtt agtagaattg acgactaagt taaatccgtc
      301 tgtgaaaatt gtagaacgta aacctgaatt agctacagat actgtaggga aaattgaagt
      361 gatacgtgat tcattcttac aactagaaag agaagataat atatttgatt taattataga
      421 tttagatttg acttcacctt tacgtgaagt ttctaatctt gaagaattaa tagatgaaca
      481 tctaaaaaat ttagataaag acgtctactt ttcagttgtt tctgcaagac gtaatcccta
      541 ttttaatatg gtaaaaagaa ttgaaaatga agttactata gttaataaat caagatacac
      601 atctaggcaa cagacacctg aagtttttga aatgaatgcc tcaatgtatt cttttaaacc
      661 tgaatttcta aaaaaaagta attatatttt tgatgggcaa tgtgggataa taaaaatgag
      721 agattatcta attttagata ttgatagtga agaagatttt aaatggttag aatttttgtt
      781 tcctaaattt ttagaagatt cttcggagct tcaagatatc tataattatt tatatcggag
      841 gtaaaaggat gagctgttat cttatcaccg gtggggctgg cttcattggt tctaccttag
      901 ccaatcattt aggagaagaa catgtggtaa tcgtcgtcga tgacttatca atgggaaaag
      961 tagaaaatct tgatatgacg aaaaatatca cttttattga gggagatgtc gctgagaaat
     1021 ccttgatgga agagatcatg aaagctaata aatttgatta tatctttcat ttagcagcag
     1081 ttgctagtgt ggcagattca gttgcccgac cagtagaaac acatcgtgtg aattttgaaa
     1141 gtgtattaat gattttagaa ttgatccgca agtatcaacc tgatttaaaa cggatcgtgt
     1201 ttagttcttc agcggctgtt tatggggatg aaccgacttt accaaaaaaa gaagagtccg
     1261 tgattcgccc attgacgcct tatgcggttg ataaattcgc tgcagaacaa tatgtgctag
     1321 attattgtca tttatatgat gtacccggta gcgcagttcg ttttttcaat gtctatggtc
     1381 caaatcaaaa tccgaattct ccatacagtg gtgtgatttc tattctcgta gatcgctata
     1441 agaaacaaat cgtaggagaa gcaacttcat ttacattatt tggtgatgga agccagtcta
     1501 gagactttgt ttatatagat gatgtaatcc aagcattact tttagttgcg aatgaggaag
     1561 ctgcactggg gaaacaattc aatgttggaa cgggtaaagc aaccacactt ttggaattga
     1621 ttcatgcaat caatgaaatt ctcggtgtag aattgacact tgaatacaaa gaagagcgtt
     1681 cgggtgatat ccatgactct ctagcagata ttactaagat ccagtcaatt ggctatcaac
     1741 cgaaatatgg tattttaagt ggaatgacta attatttgaa aactgaaatc aattaaaaca
     1801 tactaagata agattagagg gtaaattcag gctctttgtc aataaggact gatgagtttt
     1861 gtaaaatcaa atctgggata ga
//
LOCUS       DACDPS010000193         1876 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.201, whole genome shotgun sequence.
ACCESSION   DACDPS010000193 DACDPS010000000
VERSION     DACDPS010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.201"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            85..699
                     /locus_tag="IWJ07_002799"
     CDS             85..699
                     /locus_tag="IWJ07_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9334312.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(864..1688)
                     /locus_tag="IWJ07_002800"
     CDS             complement(864..1688)
                     /locus_tag="IWJ07_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334313.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQ
                     ITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
BASE COUNT          620 a          322 c          281 g          653 t
ORIGIN      
        1 ccatttaaaa agaaaagtga catatggctt cctcgtgtat gattagggtg tattttaaat
       61 agttgcagaa agtaggtaaa agttttgaga gttgcataca tacgtgtctc atcgatcgat
      121 caaaatgaac aacgccagat tgaagaaatg aaaaaattcg gtgcagagag aattttcatc
      181 gagaaacaat ctggcgcaac gattactcat cgacctgttt tccaagaagc attagattac
      241 gtccgagatc aagatatttt tattgtggaa gctattgatc gacttggaag aaattatgac
      301 gagatcatcg cttcagtcaa ttacttgaag aagaaaaatg tgaagctgat cattactagt
      361 ttaccgatca tggctgaagc aattggcaat cctctcctgg ataaattcat taaggatctg
      421 atcatccaaa ttttggccat gatcgctgaa caagaacgga ctgaatcgaa acgtcgccag
      481 gcacaaggaa ttaagatcgc aaaagcaaac ggcgtctaca aagggcggcc caaattatac
      541 agtgcagacg caaaagatcc ccaacgtcgc cttgtttaca aaagcatcgt tgaagatctt
      601 aaaaatggtg ttgcgattgc taagattgct aaagactaca atgttacgag acaaaccgtg
      661 tatagaataa aaaaagacag catggtcaat gacaaatagt tgatcatgct gcttttatta
      721 tcttctcatc tttaatttcc tacatataat tactatctat aaaattaagt cttactcaga
      781 acagataaag cataaaattc caacaaacca ttttttactt aactaaataa agagtctgtt
      841 aatataatct tcacttattt acgttaatcc cctactgtat ttgttggttc cttatctata
      901 ttttctacaa aatccttatt atcttgtgat ttagcttcgt ataattcttt gtatgtcgac
      961 acattggtta tactaattcc tatagctagt tcttgagact ctacttttct ctctagctcc
     1021 tttgacaaag tgatttgaga tttatttggt tccgttggag tcaattctat agattcttca
     1081 taaaaatctc tatacataaa gctatcatta attttataaa aaaaattaaa tgtttttagc
     1141 actgccatat aacattcaaa acattcttct atagttggtt tacttccttg aggagaatga
     1201 accaatttat tacgtttttc tatgaaattt ttaatttcct ttcttatttc tttcggaaac
     1261 tcaatattaa agtacccttt aaacatgctc cctaataatt tgtctacagg gggagattgt
     1321 aaattctcaa atattagagt taaatcaggt ttctcttgtt gataaaattc ctttacccct
     1381 atttcacagg ctgtaattat cgaaataaac ctatctctag tatttgatat atttttactt
     1441 cttttaagga gtataagaga aaaggggata ctttcgtgtc catgcatgta attaactaat
     1501 tgttgagctt cagaagttat atctctaatt atatagttaa cggaattagg aagtgtcttt
     1561 aactctaatg aaatttctat ccaattacta tcttctaatc tgtatatgct cgaagtataa
     1621 tttgattcgt cagcaagcat cgctacatcc caaacactga tcataaaatc ttttaaacgt
     1681 tcaatcattt cagcaatatt gctaaaatca gctaattttt ctttagaata aaaaaaacat
     1741 ttatatcaga atcaataaat tctatttttt ttattactat atcgtcatca ttatcttgac
     1801 catttatgtt tattgttccc tgagtgggga gtacttcttt caaattcaag ttaaatttat
     1861 attccattac ttctcc
//
LOCUS       DACDPS010000194         1869 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.202, whole genome shotgun sequence.
ACCESSION   DACDPS010000194 DACDPS010000000
VERSION     DACDPS010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.202"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..551
                     /locus_tag="IWJ07_002801"
     CDS             <1..551
                     /locus_tag="IWJ07_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HAP9334314.1"
                     /translation="KKTPMILLIIGIIFTTVGFSLSSSNEANDNNKQESSSSDTQTTT
                     SNSSNKSLTQFEEFLETNNHDWGAFLDSYYSITPATEQNTAFTKYISGKTYTFEGTVI
                     ESMTTRIAIIADKEYDDKSWNDISATPKVSYVIFAKDLNNADTFTKGDKVTFTGEISS
                     RGSNIEKSYAQWDMINSKVSKK"
     gene            664..1299
                     /locus_tag="IWJ07_002802"
     CDS             664..1299
                     /locus_tag="IWJ07_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334315.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            1598..>1869
                     /locus_tag="IWJ07_002803"
     CDS             1598..>1869
                     /locus_tag="IWJ07_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9334316.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTL"
BASE COUNT          742 a          296 c          315 g          516 t
ORIGIN      
        1 ataagaaaac acctatgatt ctccttatta tcgggattat ttttactacc gtaggtttct
       61 cgctttcctc atcaaacgag gcaaatgaca ataacaaaca ggaaagctca tcatctgata
      121 ctcaaactac tacatctaac agcagcaata aaagcctaac tcaatttgaa gaatttttag
      181 agacaaataa tcatgactgg ggtgctttct tagatagtta ttattctatt actccagcta
      241 ctgagcaaaa tacagctttt actaaatata ttagtggtaa aacatatact tttgaaggaa
      301 ctgttatcga atcaatgact actagaatag ccattatagc agacaaagag tacgatgata
      361 aaagttggaa tgatatttct gcaacaccta aagtttcata tgtaattttt gcgaaggact
      421 taaacaatgc tgataccttt acaaagggcg ataaagtaac ttttacagga gaaattagtt
      481 caagaggatc gaacatagaa aaatcctatg ctcaatggga tatgattaat agtaaagtaa
      541 gtaaaaaata aatgaaccac gccccactaa gagttagatt gtgcacaata ataaaaccta
      601 tacaaaagag tgccatcagg agtcacaatg cttgtaaagg aaatgacaaa ataggaggta
      661 actatggata atagcaatga aaaactaacc aaaaaagaat atcttgaatt aatagttgaa
      721 ggcggtattg gagcaattcc ttatattggt ggcgttgtgc agtcacttta ttttgggtct
      781 aaaaacgaga agcgtttcaa acgcatcgag aaattctaca aaaatttaaa cgaatcatta
      841 gaaaacattc gcgatcaaat tccagaagat gttttcaact tagagaacaa ggaccaactc
      901 attggaatat tcgaagggat aaatgacgag gtagaaaaat caaaggcaca aaataaaata
      961 gatatgtata aaaatcttta taagaattgt ttacttcgaa ttaacaacgc ttcatgggat
     1021 aacgaggagt attatcttct tgtactgaat caattaacat cgattgagat tcaattgtta
     1081 gcatgcttaa tgagtcgtgg aaacgaaaat ttcactggaa atatcagcat gtctggctat
     1141 tcacaagagc taatagatgg tagcttaaat agattatctg attttggtct gttagaaaaa
     1201 acgattagct ccatagtatt aggtgatgtc ggcaaacaaa atatgggcta caaaatatct
     1261 catttaggaa ttcaatttat taactcaact cttacttaag ttttctttct aatatgacat
     1321 ctagacctat cagcaatgca acaataaaaa accagacata ccaataatca acaaataatc
     1381 taaactctgg aagttcagtt attcttttaa aaatcacttt aatcataata aacacctctg
     1441 attggatgat ctaatacaca tccaattata ccattaaaac gacaaatagc acatccttgc
     1501 gccggcaagc aaaaaggatg tgcaaaaaat aataaaaccc aaacaatggg cttctttata
     1561 gttcctattg tatcagagaa agagagctga ttcaattatg gcaagattag tcaaacgtgg
     1621 aaatagttgg caatacgaaa tttcatacaa aaaagatgac ggaaaataca cgaagataag
     1681 aaaatcagga tttaagacaa aaggagaagc aaaagatgcc gccaacgaat tagaatataa
     1741 cttgaacaaa ggccttaaag gggatcgcaa aaatctatta ttatcagatt actttgagga
     1801 ttggatgcaa ctttataaag aaggaacagt atctcctatc acttatagaa aatacgaaga
     1861 tacgttaat
//
LOCUS       DACDPS010000195         1867 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.203, whole genome shotgun sequence.
ACCESSION   DACDPS010000195 DACDPS010000000
VERSION     DACDPS010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.203"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..425)
                     /locus_tag="IWJ07_002804"
     CDS             complement(<1..425)
                     /locus_tag="IWJ07_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP9334317.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
     gene            complement(534..1565)
                     /locus_tag="IWJ07_002805"
     CDS             complement(534..1565)
                     /locus_tag="IWJ07_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9334318.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          576 a          313 c          322 g          656 t
ORIGIN      
        1 ggaatctgtt ttgattttgt tccatttgaa atttctctca ctcgcttttt tgtctcatca
       61 tcaaataaaa tgaaagggtc ttctaaacga tcgataaata cttccattgt tgttgttggt
      121 agatgataag aagtcatgac aggcactgca ccaaggtaag acacagaaac agctagaagg
      181 tatgtgtcaa aagcggaact cttataaata ataactttgt ctccggattt cactccaaga
      241 tgagccaatt gataagcccg tttcaacaca ctttcgcagc attttttgta agttgtttca
      301 agtcctaatt ctgggaaagc tgaaagtggc tcatcaaaga taatcggggt ctcggaaaag
      361 ttagctgcag cttcttgaaa attcgtaaat aagttcaaag gggaatacgt tatggtctcc
      421 atcattcaca ctccattatc ttttgttaag taatatacaa attcaagata atcgtaacat
      481 aatcctttat ataataacat agtttattgt gaaaaaaaga tcatacattt gttttatatg
      541 gaagaaaccc gaacgaatgc cgctaattcg tgataagaaa aatcaaccat cttttcccag
      601 atatcttgaa aagaatggga tgagaggtat cgattaccac ataaataatt taccgcgtca
      661 gcatcatcta aatatagttc tgcataataa cgatatgttt ttctgcatag ttctggcgat
      721 acgtttttat atactgtaat tttgctcaaa atttggcgaa tagtccgagg agaaatttgc
      781 tgatcatgtg aggaacgaaa aacaaaagaa ttttcgtccg tattttgtgc tgttaactta
      841 aaatagttct ccagaaatgg catcgcttct tttggtattg gtactttatt cagtctgttt
      901 tcttttctat tcagattaag aatctgcttg gtcaaagata catctttacg tttaagttct
      961 actacttcat ggatctttaa tccgcaatac agaagaatgg cagtaattgc aaggttcctc
     1021 ggtctattct tttcttcaaa gctagcttct ctgcttgttt gaaagccttc aatcgggata
     1081 aacaaatgct caaaatcttt gaaaagttct gcatcgatat atcgcggagc tgctttggtt
     1141 gtttccggta aacttgtacc caaaagagaa atctcttctt caatcagtcc tttttgatac
     1201 aaaaatcgta aaaaatgatt taatgaatat tgcttccttg aaatcccgta atagctattt
     1261 tcaaacaatt gctccgtttc ttggttcgct gtagtttgga acgaacgagt atgtcgatag
     1321 attttagcta agaaattctg ataatcttgt ttttctaaat ggataatcag ctgatcatcc
     1381 acttcttcag aaaatacttt tctcaaataa tcagtaaaca gatgaaaatc ttttgcgtat
     1441 tcatagcgac tgcctaatga aagggaagaa cgattttctt ctaaaaactg ttgaaagacg
     1501 gaagggagca agtgtgtacg ttgctttaac ttttcttgca gttgttttct agttttgctg
     1561 tccattacaa attccctcca atagtgttca ttttctatat tttaccgcat ttcatcaaat
     1621 gaagatagac agtttgtctt gatcttagga agcaaatttt taactaagta ctagctaaaa
     1681 gatatgaagt aagaagatac atttttagtt attttattag tgcagtcatt cttttgatct
     1741 tttaattgat ttatcctctt tttagctatg tggttacttc atgagcaatg aaatggattt
     1801 ggttcgttct aatggcagga ggctctttct tttatttccg aaaaattaga aaacattttt
     1861 tcagaat
//
LOCUS       DACDPS010000196         1847 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.204, whole genome shotgun sequence.
ACCESSION   DACDPS010000196 DACDPS010000000
VERSION     DACDPS010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.204"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(355..1647)
                     /locus_tag="IWJ07_002806"
     CDS             complement(355..1647)
                     /locus_tag="IWJ07_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9334319.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
BASE COUNT          580 a          369 c          307 g          591 t
ORIGIN      
        1 tctgtgatac ttattagcca ttaaactatt ttacccacat agcatatatt ttgaaatcgt
       61 tcattccgta aattcaacta aatatgccta aaaaaataaa aaatctaaga tagaataaga
      121 tttattcagt cttagatgga tttatagcaa ctttttcgta acaactttaa aaaatacgaa
      181 aagaaagagc tgttttactc tgactacgtt gctaaattgt cgtttaatca ttcatttaaa
      241 ccaaatcttt ttttatagaa ttcgttttaa tcctcctgaa tccaaaaact atgaaaaccg
      301 taattaaata gttaagagtt tttaaccacc ataattctct cggctatttt gaaatcagat
      361 aaattttgat cggacacata gttgaataaa cctaccgttg ttacggaagg taaatcgcat
      421 acttttcatt ctagaggaga aggtctcgtt gatcattaac tgattaagca ccatagccca
      481 gttttgaatt cgaccacctg accacttcgc gtgtaattct ttcgtgcgta aataaagtag
      541 ttttaggagt gcattctcat tagagaacgc tccttttttt gtgacttttc tgaagctgga
      601 gtggacactt tcaaccgcat tggtagtgta cataattttt cgaatggcac taccataatc
      661 aaatagttgt tcaacatgtg caaagttccg tttccagaca tctacagcac cagaataatg
      721 agaccaccga ttttgaaaac tgtcaaaagc agcatgtgca gcgtttagag aagaagcacc
      781 gtagaacttt ttcatatctc ggcagacttc cttatagtcc ttacttggaa tatagcgcaa
      841 tgcatttcga acaagatgaa caatacagcg ctgaacaatt accgatggaa agatcgcttt
      901 tgcgccttct tcgaggccag aaacaccgtc catcgaaatg aaaaagacat cttcgacacc
      961 acgtgctttc agttcatcaa atacttgcat ccagcgattt ttagattctg tttgatttaa
     1021 ccataatcct aaaatctcct tatttccttt gagatcatag ccaagaatgg tgtatacagc
     1081 atattctttg gcttcataat tttctcgtaa agtaacatac atacaatcaa cgaatagaaa
     1141 ggcataacac tttgctaggg ggcgggcttg ccattcttcc aattcaggaa ggacagcgtc
     1201 agtgatatct gaaatcattt catgggaaat atcaaagcca taaatagctt cgacggttgc
     1261 ggcaatatct cgttgactca ttcctcgtgc atacatggaa agaaccttcc cttcgatgtc
     1321 ggagacatct cgttttctct taggaattaa ctctggttca aaggaagctt cccggtctct
     1381 aggaacatca atagctactt caccaaaact ggttttaagc gttttagttc catagccatt
     1441 tcgacggtta tcgtgttcct taggctcttt agaatgggca tcataaccta aatgattatt
     1501 caattctcct tgaagcattt tttcaaaaag gggcccaaac acatctttca aagcatcttg
     1561 catgtcatcg acagattcag gttgataggc attcagaatg gattcagcta acttttcggc
     1621 atcaggattt cttttctttc tagccatcgt gtaatcacct cttgatctta ttgtagaaaa
     1681 agaaaaaccg cgaagcaacc acctgcctag gattaatggt tacttacacg gtttatatta
     1741 cactctcatc attaaaaaag tatctttcga tattactaaa caatgaaatt atctatttga
     1801 aatttaaatc tgataatata ttgagtgttc caatgagggc tttgatc
//
LOCUS       DACDPS010000197         1805 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.205, whole genome shotgun sequence.
ACCESSION   DACDPS010000197 DACDPS010000000
VERSION     DACDPS010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.205"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..112)
                     /locus_tag="IWJ07_002807"
     CDS             complement(<1..112)
                     /locus_tag="IWJ07_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9334320.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDY"
     gene            complement(530..1717)
                     /locus_tag="IWJ07_002808"
     CDS             complement(530..1717)
                     /locus_tag="IWJ07_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAP9334321.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          540 a          380 c          327 g          558 t
ORIGIN      
        1 tgtaatctat tttagtgaca gtcaggtata gccaagtaac tagaagatca gtggaacctg
       61 caccataaaa gtttgagttt gtcggtccgt tgacagttaa aagaaaaatc acgttgaagt
      121 tattgatatt tccgattacg ccgcttcgat ttggtcacga tccaaatttt ggataatccc
      181 tgtagaaatt agcatagtag ctgggatacc aattcacata ttgacaaaga taacagagac
      241 ttttacataa aacggatcaa tcaggaaagg aattgctcga tccagcccca gttttccaac
      301 aatgtgttaa ctggcctagc gtcattcagc aaattatgca tgatgaggag cgaaatgaaa
      361 gtcaagtcca caatcttgtg taaaagttca acctcatcaa aagcacaatg ttttgcgttg
      421 tgaacctaac tgccatgcct ttctttctga ggagagaggt caggagcagt agcgcagcga
      481 ctcttgatgt ctctcctcag aaagaagaga ctcgcacagg ttcatggtgc taaatgttat
      541 tcgaattaaa ttctaaatat tttcgtggag aagccagcca ctcagtgtct ttttccatca
      601 aaagtgttcc aattatccga gtgatggaat tgatatttgg aaaaatccga atcactcgtt
      661 ctctccggcg aatctcttcg ttcaaccgtt ccaacacatt ggttgtacga atccttcggc
      721 ggatattttc aggaaaagac aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt
      781 cgcaagcagc acttaatttt gaatcacaac catatttttc caagaagtgc tcttttcttt
      841 cacgtgtcaa ttcaagttca gaggctttaa agatactctt caactcgtct tttacatcag
      901 aagaaacttt ctttggcagc ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt
      961 gccagctcgc acctaaaaag ttcttgcgaa cagccttaac taaccctttg tgtgcatcag
     1021 aaatgaccag ttttacaccg aacaaacctc gttgaacgaa cgattgaaaa acggtatccc
     1081 aattatcttc cgattcgccg tcttgaacat caaaaccaag aatcgtacgt cgtccatcag
     1141 aatcaatgcc gataccaata tatacacctt ttgagacaat acgatgattt tcccttactt
     1201 tcatatacag ggcatcacac atgaaaaatg gatattccga gccttctagg ctccggtttc
     1261 tccactcaaa aacaaatgga tccaactgtt tcatcagatt tgaaacgaat gatttagaaa
     1321 cagtcgcacc atccgttagc agttcaactg ttttagttac tttgcgagtg gagacaccag
     1381 aaatgaccat ttcaatcatg gcagcgatca aagctttttc gcttcgctga tatctttcaa
     1441 agatagtagg actaaactta ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa
     1501 ttcgagttgt aaacgaacga tcgtagtaac catttctttg tgattgccgt tcttctgaac
     1561 gttcataggc atttgcctga atatattgat ttcgttgttc ttccatcagc tgattaaaca
     1621 tgatagtcat aagtgttttt gcggtatcat tcgcaccgct ttcgtttata attgcttgaa
     1681 tttcttcgct ttcaaaagta aaatgtactt gagtcattga tatcagtcct attcgagtat
     1741 gttttctgca aaaaacattg tactcctaac tgagatcaat ggcttttttg atgcttcact
     1801 tttac
//
LOCUS       DACDPS010000198         1795 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.206, whole genome shotgun sequence.
ACCESSION   DACDPS010000198 DACDPS010000000
VERSION     DACDPS010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.206"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..277
                     /locus_tag="IWJ07_002809"
     CDS             <1..277
                     /locus_tag="IWJ07_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9334322.1"
                     /translation="KWLFYLSRLIIQTALLKNLPETLDAQLRTKLQNLLTYEEGIYNA
                     MIYPYSNGKIEAKIPHIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
     gene            436..1731
                     /locus_tag="IWJ07_002810"
     CDS             436..1731
                     /locus_tag="IWJ07_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9334323.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT          577 a          347 c          321 g          550 t
ORIGIN      
        1 taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgttattaa aaaatcttcc
       61 tgaaacactt gatgcacaac ttcgcacgaa acttcaaaac cttttaacgt atgaagaagg
      121 tatttacaat gctatgattt acccgtattc taatggcaaa atagaggcca agattcccca
      181 tatcaagaca ctgaaacgat tatcttatgg atttaaatca tttgagaaca tgaaaatcag
      241 gatttttttg atcaatcaat tgattcaagt gaaataacaa aaaaatctga gacagaaggt
      301 cagtccttct atcggctcta tactttttga tgttggtgaa cttcaccaac atcttttttg
      361 tataaaaaaa tatagaaaag acaccctgaa atcctttaga attaatttgg ctaccaattc
      421 ataggaggat tcaagatgtc tcatactcag cttatcaaag ataccctaaa tattctagac
      481 ctaaatatct atttcgagga aaattgttta actaaagaaa aatacaaagg gcaaatttgc
      541 ttgatttaca aaggaactct tctgtacaag ccagaagagt gttcccattg tctttgtgtc
      601 gtcccctcta ggatcattcg ttgggggacg acaaccgttc ggttgttact gaatgatgtg
      661 tctgagtatc gcacttatct ggagctgaag aaacaacgat ttaaatgtaa gtcttgccaa
      721 agaacattcg tagcggatac ctctgttgct gaaaaacact gtttcattag tcaaaaagtt
      781 cgttggtctg tcattgctcg cttaaaagag aatacttcca tgacggaaat cgctcgtcag
      841 aaaaatattt ccacttcttc ggtctatcgt gtcatgaagc gattctaccg cccattgaat
      901 cctttcaaac agactttgcc aaaggtctta tgctttgatg aattcaaatc ggttcgaagt
      961 gttgccagtg caatgagttt tatcatgatg gacggacaaa gccatgcatt attggatatt
     1021 gtcgaaaatc gacgcttgcc ttatttagaa cgctattttt ctcggttttc attagcgact
     1081 cgtgaagccg ttcaattgat tgttattgat atgtatgcgc cttatgtttc gttggtgaaa
     1141 aagcttttcc cgaatgctca gttgatcatc gatcgtttcc acatcgtcca acatattggc
     1201 agaactttct tgaatcatcg agtgaaagaa accacggtac gacttaaagg aaattcccac
     1261 tctcaacgag ggttagggaa aaaattgaag cgctattgga aactcctaca aaaagaagaa
     1321 gcaaagttaa actatgaaaa acgggtttgg cgtgccagtt ttaaagatta tttgactgaa
     1381 agtgagattg ccgatcgact gttagctgct tgtcccaact tacgccaagg ctatcagtta
     1441 taccaagaca ttctttatgc ggtaaagaaa agagatcagt cactatttga ggattgttta
     1501 actagagaca ttagcggtct gccagaaacc tatacaacca ctttacgaac gtttaaaaag
     1561 tttctgccac agatccaaaa tgcgcttcac tacagctatt caaacggacc attggaatgc
     1621 ttaaacaacc acatcaaagt attaaaacgc aatgcgtacg gctttcgcaa cttttacaat
     1681 ttcaaactac gaattatgat tcgtcatggc aaaacatttt taaccaaata aacagaacag
     1741 aaagaggttt tcttctttct gttctgtcca aattattcta agatttctct ttacc
//
LOCUS       DACDPS010000199         1783 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.207, whole genome shotgun sequence.
ACCESSION   DACDPS010000199 DACDPS010000000
VERSION     DACDPS010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.207"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            189..386
                     /locus_tag="IWJ07_002811"
     CDS             189..386
                     /locus_tag="IWJ07_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334324.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            492..974
                     /locus_tag="IWJ07_002812"
     CDS             492..974
                     /locus_tag="IWJ07_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334325.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1032..1628)
                     /locus_tag="IWJ07_002813"
     CDS             complement(1032..1628)
                     /locus_tag="IWJ07_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP9334326.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
BASE COUNT          560 a          271 c          357 g          595 t
ORIGIN      
        1 atatgtttta attttttgaa aaaaattctt ctaaatacaa cagctgtttt aattgtgaaa
       61 acgaaaacag tacgctataa tgcctgtaag gttgattttt aactgtaaat aaaaatcaat
      121 gagttaaatc tccaaagaaa tagcagtggt aatgtggaga aaagttccag cagtttagga
      181 ggattttgat gacggaagaa atgattaatt taggagaaca atatgcatgc aaaccaattg
      241 gttttacaaa aacggtcatt ggtgaagttg tatcaaaaat gacaaactgc gccgtggtca
      301 aagttgccca atgtgctgca gaggaccaag aattattaga tgaaaaagca agcatggttg
      361 ttgcaaaata tgatacattt gaataaagaa aacaattgga cgtgcggcgt gctgcaagtc
      421 caatttcttt gagaaggtaa atcagtattc ttttcctatt ttctggagaa gaaaaagcga
      481 ggcgagttgt catggtaaca ttctttacgg ttctattgtt tttattcgtt gttgtgatgt
      541 ttttatttct ttggctggtt cgaaaagaaa ttatttatcg gactgtgcga aatcgatggg
      601 tctacttggt gatacccttc ttagtgtcac tagtcatttg gtatacactc atcagtcatc
      661 ctacagcaga tgaattggca aaagggatct tgtcagcaat gatttttatt agttttttgt
      721 tggacagtag aggaatcaca gaagaaggac ttgttttgaa tagctttgac aaaaaaggtg
      781 ttcctttttc tgagatcaat aaaattgtat tatatcagcc aaaaggttct aaaatcgtaa
      841 aaatgaattt tttccgaaac ggatggcgag gaccgatgct gaaattttct gcatcattag
      901 aagaattagt tccatttttg tcccaacgct taaacgatga ggcagagatt gatattatga
      961 ttgatccaga ataaaaacag aggttgcgac aagatgtttg tcacaacctc tgttttttat
     1021 ttcaaataaa attatccaaa tacgcggaag cgatcggcat cgttcatgta ttctttttcc
     1081 ccagccggtg tttctggtga accgaatact aattgcgaac gtagtttcca gtttgaaggg
     1141 atattccatt cttttgcaac tgcttcgtca ataactgggt tgtaatgttg caagtttgcg
     1201 cccaatcctt tatcaactaa tgctacccaa gtattagctg ttgcgattcc gtttgattgt
     1261 tctgaccaat ctgggaagtt gtctgcatat aattcaaatt gttcttgtag accttttacc
     1321 acgtcagtat ctttgaagaa taatactgta ccataaccat ttttgaaacc agctaatttg
     1381 ttttgtgtat ttgggaaagc ttctgctgga gtcaatgggc gaagtgcttc ttcagtgatt
     1441 tcccaaagtt tttcatgtgc atctccgaaa aggataactg cgcgtgttga ttgagcattg
     1501 aaagcagttg ggctttcttt aattgcttct ttgatcaaag ttgttaattc ttcattagaa
     1561 agtgaaacat tacgtcctag atgatagatt gaacggcggt ttttcaaagt gtcagtaaaa
     1621 gttgtcataa taaaatttct cctttgattt gcgttttgca tttttttaat tgattacgaa
     1681 atgaagtata gcaccttaca aaaagtaagt aaacagatat gctcacaaaa aataagcgaa
     1741 gttcaaaaaa caattttaat aagaaaaatc gttatgtaag gat
//
LOCUS       DACDPS010000200         1770 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.208, whole genome shotgun sequence.
ACCESSION   DACDPS010000200 DACDPS010000000
VERSION     DACDPS010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.208"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            144..494
                     /locus_tag="IWJ07_002814"
     CDS             144..494
                     /locus_tag="IWJ07_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9334327.1"
                     /translation="MSRLIIQTAFYKAIIPENLDEVKLYLWEDNGEIVGFSGVSAEEL
                     VMLFLDPDFIGKRYGSQILIELIETEDIKRIDVNSQNKYAKHFYLSHGFEIESEEDLD
                     GFGKPYPITHLVKK"
     gene            908..1726
                     /locus_tag="IWJ07_002815"
     CDS             908..1726
                     /locus_tag="IWJ07_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP9334328.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
BASE COUNT          577 a          233 c          369 g          591 t
ORIGIN      
        1 gcaaccggag tttcttgatg aaaatatgct tctatcatta caagctcgtt tggtgtaaga
       61 tgggtataag tcatttatgt tcactctcct tgtatgcttt agcgggtatt acaatttgag
      121 tgtaacataa atggcttttt tatttgtctc gcttaattat acaaacggcg ttttataaag
      181 caatcattcc tgagaactta gatgaggtta aactttattt atgggaagat aacggagaaa
      241 ttgtcggttt tagtggtgta agtgcagaag aattagttat gctatttctt gatccagatt
      301 ttattggtaa gcgatatggt agtcaaatat taattgagct gattgaaact gaagatataa
      361 aaagaattga tgttaatagt caaaataaat atgcaaaaca tttttattta agccatggat
      421 ttgaaattga atctgaagaa gacttagatg gttttggtaa accataccct ataacgcatt
      481 tagtaaaaaa ataattcatg aatggtttat tacctgattg aacatgaata aaagtaatat
      541 ttttattttg atggcctatt tctaggttat gaattatcca gatcaaattt gtgtattaca
      601 ttcagtaata aggtgcttat cttaggatca acttatgata ctagctgacc aaggatgtac
      661 cagttataga aaatcagtta aagtaaaaat gataaaaatc ttatgtcaac actgatttag
      721 gctgacctgt gggttaggag aaagacaggt cggtagcttt taatacaaaa aatggcttgt
      781 atgtatctag atagttaact gaaattagaa atgttcttat ttttaacgga gtaagaaaaa
      841 taggctgctt taggtctgaa tactttttca atataatgaa gatatcaaag gcgacaggag
      901 tgtttttatg aataagaatc ctcataatct aaagttggta gcagtagata tggatggaac
      961 atttgctcgt actgattaca catacgatat cccaaggttc aaggctgttt tatcgcgaat
     1021 gaaagctgtt gattgtcaat ttgttgttgc aagtggaaat caatactacc aattgagatc
     1081 gttatttcca aattattatg atgaattaag ttttgttgct gaaaatggtg cttttgttaa
     1141 agataaaaca gaggtactat tttcagccga tatgcctaga aaagcgatcg aggtggtcct
     1201 tgatttatgt aaagactatc ctgaagtaaa aaatgtgatg tgtggtctga atagtgccta
     1261 ctgtgaaaga ggaaaggtga gtcaagcatt ttttgatcta accaatatct attaccatag
     1321 attagaatgg gtagctgatt ttgcagaagt tcaagataaa attttaaaat ttgctccgac
     1381 tatgcctgtt gaaaaaaccg aattttacgt tgaattgttc cgtgaaaaat tagccgggct
     1441 attagtacct accagtagtg gtcatggttc tgtagacttg attctttctg agtgccataa
     1501 aactttcggc ttgaaaatgt tagttgagcg cttggggatt aatcctgacc agtgcgttgc
     1561 ttttggagat gggggcaatg acattgagat gttggagtat tgtggtctga gttatgaaat
     1621 ggataatgca acggaagctg ttaagcaagt tgctaagcat caatgtcctt caaatgacga
     1681 agacggtgtt ttagtgacgc tcgatcgatt gttccctaac acgtaaacac taggtaattc
     1741 atcaaaaaac ctcgatataa gaaatattcg
//
LOCUS       DACDPS010000201         1765 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.209, whole genome shotgun sequence.
ACCESSION   DACDPS010000201 DACDPS010000000
VERSION     DACDPS010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.209"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            244..1722
                     /locus_tag="IWJ07_002816"
     CDS             244..1722
                     /locus_tag="IWJ07_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9334329.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT          646 a          244 c          311 g          564 t
ORIGIN      
        1 tttttgtggt tgtttacgca aatggcgcaa atcttgtaca ttttaacaca ataaaacaaa
       61 aaatatagaa tatttcctca taaatttttc cattttttgc aatattctca aaaaaaagat
      121 aaaaaatgag aataactgat gaaaaaacaa gcataatcca tcataaaatc agtataggtg
      181 aaaagatgaa ctttcatctg catatcaagt aattggtatg aggcattttt tgaggtgtag
      241 gatatgaagt tggattacct tatagaagag gaagatagag aaaaactgct gctagctaga
      301 tatttagaat ttgagaaaaa gaattatgta gtgctgccta agttatatga aacattaggt
      361 ttgtctaaat ttaaaacaag aaattatatc atagaattaa ataaagaatt tactgaattc
      421 aaaacaaagc cagagatcca aatttcagaa gaagcagaga tccatgtcga acgtctgaat
      481 ttgacggata tcaaaaattt aagaagaatc tactttcaac gatcaaaaat tgctcgactg
      541 ttaacggaga tcgtggattc aaaagtaacg atcgaaaaat ttgcagagaa tcatttttta
      601 agcatatcaa gagcctatgt aaaaagaaga gaattagtct cttttcttga acagcatcaa
      661 ataaagttaa agaaaaacat attagttggg gaagaaataa acatacggaa cgtattattt
      721 ggggtatatt tcggggtctt taatggtttt tctatgccat ttagagaaag cgtcatgaat
      781 gaagcaaaga gtatcgtgca atattttgaa tatctgttca atttaaaact gtctgcaaca
      841 aaacgaatca aattcaattt gctaaccact attagtttgt tgagggccta tgggaatcaa
      901 ccaatcggaa aacgtttctt ttcagatgaa tactttaaga gctcagaagg aatgaagatc
      961 atcaaggatt tatcacatat ttcttttctt catatcccta aagagcagat cattgatgag
     1021 atttcttata tcatgttgtt tttgatcacg gaagaagaat ttgaattaga tggcgcattt
     1081 ggacttgcgg aagctcattt tcatgatatc cacatgatta gtcaaaaaat gtcagtggaa
     1141 ttgattgatc aattaccatt ttcgattgaa atctctgaag aagaaagact atatatacga
     1201 aaagaacttc gtatgggtat gatgcatgtc aatcgaaaaa attggatatt cgactttgag
     1261 atgactagct ttacgtctaa aaagcagcta cagtttttct tcgagacgta tccaatattc
     1321 agtacagcaa tccaaaaggt catacaagag aataaacgct atttcttggg aaaagatgaa
     1381 cgtttcatga ttcgcttatt ttatgattat ctctttttat tggtcggtat ttgtccggta
     1441 tcatattttg aagcaccgat tcatgtctgt attgattttt cgcaaggatc tgcttatacc
     1501 gattacatta tttctcaagt gaaaggattt aaaaattaca atattgtgat tgagagtcga
     1561 atcacttctc gtacgaatat ttatttatct gattgtttac ttgaaaaatt taataaagaa
     1621 cagataattt ggaaaaatcc accgactcca gatgactggg aatattttgg aaataccatc
     1681 atcagagaaa aaaatagaca tttaactgaa aaaatcagct agtatttatg gatggcagct
     1741 ttgtctcaca tatttattag ctgat
//
LOCUS       DACDPS010000202         1757 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.210, whole genome shotgun sequence.
ACCESSION   DACDPS010000202 DACDPS010000000
VERSION     DACDPS010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.210"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(142..>1757)
                     /gene="uidA"
                     /locus_tag="IWJ07_002817"
     CDS             complement(142..>1757)
                     /gene="uidA"
                     /locus_tag="IWJ07_002817"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAP9334330.1"
                     /translation="GWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNGKFITQHK
                     GGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKKENLVNFD
                     FFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILIAIYDEEA
                     QLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGIRSVQVTN
                     GKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTSHYPYSEE
                     IMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKTVIKELIE
                     RDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYLKSTPDVC
                     KVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTEYGADTVA
                     GFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILRVQGNKKG
                     IFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
BASE COUNT          556 a          360 c          210 g          631 t
ORIGIN      
        1 gaaagcccct ttttttcgaa aaaactagaa aaaaaacata aaaatattta tccttcttta
       61 aacagaaaat ctttctataa aaaataaaaa aaacgattag gctaggctaa tcgttttttg
      121 aacattctta aaaaatccaa attatttttt ataactgaaa ttgggtattg atgaccatcg
      181 tttctgtaga tagtaagcaa tcattttagg ttttctatct ctagtaaaga ttcctttctt
      241 atttccttgc actcttaaaa tcccttgact tgttgcaaaa tctgcaaaat tccacacttg
      301 ttcaccaaca aaattttcac atgtatctat tacttcgtga tttgcttcgt aataagccac
      361 ttgatactct tctgtaaaca ttgttggtac tgtatcatga aatccagcta ctgtatctgc
      421 tccatattct gtaaacatta ttggcgtatc aggacatctt tccatccatc catctagctc
      481 ttttttcaac agatattttg catgtgccag atcccctccc gaaacatacc aaccataata
      541 acgatttaaa cataaaacat cgacaatatc tcctacctta caaacatctg gcgttgattt
      601 tagataagta acgattgtta caggtctttt ctgcggatca agttctttag ttaattcaac
      661 taaaggttca aaatattctt tagcaccttc ggaatccgaa tctggttcat ttgcaataga
      721 ccacattaca acacttgggt ggttcttgtc gcgttctatc aattccttga tcaccgtttt
      781 atgagaattt tctgttcgta tctctttcca cgtattatgt ttttccacat tatcatctaa
      841 taaagtggcc atgaaattta aatgtaaccc tactgcaggt acttcatcaa taactacaat
      901 accttctcga tctgctaatc gcataatttc ttctgagtaa ggataatgtg atgttctaaa
      961 agaattagca cctatccatt tcattaaatt aaaatcttta atatttacag cctcattcaa
     1021 accacgtcca ttaacatacg aatcctcatg cttaccaaat cctttaaaat aaaaaggttt
     1081 attattaatc aaaaatttgc cattggtaac ctgaacactg cgaataccaa attcttctgt
     1141 ataggtatcg atcactttat tatcatcaat taaactaatt ttcatactat ataaataagc
     1201 gtctagaggt tcccaaaaaa ttggattctt aagctgaatc attcctttct ttccttcagc
     1261 ttcagctaca agttgagcct cttcgtcata tatcgctatt aagatttttc cggtccattc
     1321 tgtatcgata ttgaattcta cacttgcatc caaatcagtt attcttgtat taatagtaat
     1381 atcacttata tagtctttcg gagttgtata taacttaaca ggtctatgaa tacctgcata
     1441 attgaagaaa tcaaaattaa ctaagttttc cttttttata tccccatttt cctgtactgt
     1501 ttccttcata atccccacag gtaaagtact atcgtcaata atattgttta cagcaactgt
     1561 aattcgatta ttcccgtctt ttacaaaccg attaatttca gactcaaatg gtgtaaaccc
     1621 acctttgtgt tgagtaataa actttccatt caaaaaaact tttgcttcat gtgttgcaga
     1681 tccaaaacga agaactaatc tttcttctaa taatctttta ggaagagtca cttctttttc
     1741 ataccacacc catccaa
//
LOCUS       DACDPS010000203         1730 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.211, whole genome shotgun sequence.
ACCESSION   DACDPS010000203 DACDPS010000000
VERSION     DACDPS010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.211"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..414
                     /locus_tag="IWJ07_002818"
     CDS             <1..414
                     /locus_tag="IWJ07_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP9334331.1"
                     /translation="KPFLSARDTIKALKKYGKIIIVSSANKEAVQEEWERHELLSLVD
                     ELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFFYPILVNKEKES
                     WENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            427..>1730
                     /locus_tag="IWJ07_002819"
     CDS             427..>1730
                     /locus_tag="IWJ07_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HAP9334332.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRP"
BASE COUNT          631 a          201 c          356 g          542 t
ORIGIN      
        1 aaaccctttt taagtgctag agatactata aaagctctga aaaaatatgg gaagataatt
       61 attgtaagct cagctaataa ggaagctgtt caagaagaat gggaaagaca tgaattatta
      121 tcattagttg atgaactgtg ttgtcaagat aaaggaaaaa aagaagacat tattcgaagt
      181 gtaattgaaa atggttgtga tttggataag atattaatga ttggtgattc tcctggtgat
      241 ctagaagctg caaataaaaa taaagttttc ttttatccta ttttggtcaa taaagaaaaa
      301 gaatcatggg aaaacttaag aactgatgtt ttatttaaat gtttatcggg agattatgta
      361 gatattgaac aaaaatatat agatactttt tggaaaaatc ttactaataa gtagaggaga
      421 aagaaaatga ctataaaagt agatttacga aaaaaaccat ataatttaga tgaagatgct
      481 atttcttgga ttgaagagac gattgattct atgactttgg aagaaaaaat aggtcaattg
      541 tttgttaata tgggatctag tcgtgataaa aattacttga gtaatatggt caatcagtac
      601 catattggag ctgtacgata taatccagga actgctgatg aggtatatga tcaaaactat
      661 atcttacaaa cagaaagtaa aattcctcta ttaattgctg ctaatacgga agcaggtggt
      721 aacggagctt gtacagatgg tactgaagtt ggtgtagaag taaaaattgg tgcaacaaat
      781 gatgcgaaat atgcttatga attagggaga gtttctggga ttgaagcatc ggctattgga
      841 tgtaactgga gttttgctcc tattgtagat attaatagaa attggagaaa tcctatcatt
      901 tctagtcgat cttttggaca aaaccctgat aaagttttag aacttagttt agcttatatg
      961 aaaggtattc aggagagtgg aattttgcct gcagctaaac atttccctgg tgacggtatt
     1021 gacgaacgag atcagcatct atcttttagt attaatagtt taggtacgga agaatgggat
     1081 aattcttttg gaaaagtata ctcaggctta atagaagctg gattaccttc aattatggca
     1141 ggtcatatcc atatgccagc ttattcaaaa cattttaatc ctgatctgaa agatgaagat
     1201 ttacttcctg caactttatc aaaagaatta atcactgact tacttagagg aaaactcgga
     1261 tttaatggat tagtagttac tgatgctagt catatgttag gaatgactgg ggcaatgaaa
     1321 cgatcagaat tattgcccac ttctattgca gctggatgtg atctatttct attttttaat
     1381 gatccagatg aagactttaa ttatatgatg gaaggctatc gtaaaggaat tatcactgat
     1441 gatcggttat tagatgcctt aaaaagaatt ttagggttaa aagctaaatt gggattacac
     1501 aaaaaagcaa aagaacaaat tttattacct aagcaagaag ctctggaaaa aattggttta
     1561 attgaaaata aatcaatttt taaagaggtg gctgaccaag caattacatt agttaaagat
     1621 aaacaagata tttggcctat tgtaccagaa aaatataaaa ggattttact agtagatgta
     1681 catggggtaa aaggtgggtt tggtgaagta gttgctggaa atagacctca
//
LOCUS       DACDPS010000204         1711 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.212, whole genome shotgun sequence.
ACCESSION   DACDPS010000204 DACDPS010000000
VERSION     DACDPS010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.212"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..957
                     /gene="uxuA"
                     /locus_tag="IWJ07_002820"
     CDS             <1..957
                     /gene="uxuA"
                     /locus_tag="IWJ07_002820"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP9334333.1"
                     /translation="WPRERIKALKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIE
                     NYKQTIRNLAEFGIEVICYNFMPVFDWVKSDLDYHLDDGSSTLAYISKNIPTDPEEII
                     RKVENSTNNFELPGWEPHRLSQIKQLLEAYKEVDEAKLRENFAYFLKAIIPTCEEVGI
                     KMAVHPDDPPYSIFGLPRIVKNRDDLEWLCNVVDSPSNGITLCTGSIAERPDNDVYEI
                     LSEFCQRDRIPFAHVRNIKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYI
                     RPDHGRMIWGETGRPGYGLYDRALGAVYLNGIWEALEKGASK"
     gene            1031..>1711
                     /locus_tag="IWJ07_002821"
     CDS             1031..>1711
                     /locus_tag="IWJ07_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP9334334.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAEHQPIIDYHCHLDPACLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGAGNDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLKMMDVKAICTTDD
                     PTDDLRYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAGIPIKNFNGL
                     KQALSQRVD"
BASE COUNT          576 a          243 c          362 g          530 t
ORIGIN      
        1 tggccgagag aacgaattaa agctttaaaa gatgaagtcg aagccaatgg ccttaccttg
       61 aaagttattg agagtgttaa tgttcatgat gatatcaaaa ttggtttgcc aacacgagat
      121 caatacatcg agaattataa acagacgatt cgcaatttgg cagaatttgg gattgaggtt
      181 atctgttata attttatgcc agtctttgat tgggtaaaat cagatttgga ttatcattta
      241 gatgatgggt cttctacgtt agcctatatt tcaaaaaata ttcctactga tccagaggaa
      301 attattagaa aagtagaaaa ttctactaat aattttgaat tgccgggttg ggaacctcat
      361 cggttatcac aaatcaagca attattagaa gcctataaag aagttgatga agcgaaattg
      421 cgagagaact ttgcatattt tctcaaagca attatcccga catgtgaaga agttgggatt
      481 aaaatggcag ttcatccaga tgatccacca tattctattt tcggattacc tcgtattgtt
      541 aaaaatcgtg acgatttaga atggttgtgt aatgtagtgg attctccttc taatggtatt
      601 acgttatgta ctggaagtat tgcagaacga ccagataatg atgtctatga aattctatct
      661 gaattttgtc aacgggatcg gattcctttt gcacatgttc gtaatatcaa atatttaagt
      721 gataatggac tcgattttta tgaagcgccg cataaatcag aatatggttc tctagatatg
      781 tataagatat taaaaacaat gtacgataat ggatttaatg gctatattcg tccagatcat
      841 ggtcggatga tttggggaga aaccggtaga cctggttatg ggttatatga ccgagctcta
      901 ggtgcagttt atttaaatgg tatttgggaa gcgcttgaaa agggggcgag taaataatta
      961 tataatggag tctatttaga agaaaaattt tctcttctaa aaactaaggg aaggaaagga
     1021 gtgcttaaat atgtttttag ataagaactt tttattagag aatgaaatag ctaaaagact
     1081 ttttcataaa tttgcagaac accaaccaat catcgattat cattgtcatt tagacccagc
     1141 atgtttgcta gaaaataagc aatacaagaa tttgactcag gtatggttaa atgataaagg
     1201 agctggagat cattataaat ggcgattgat gcgagctaat gggatagaag aagagttgat
     1261 cagtggtgca ggtaatgatt atgaaaaatt tttagctttt gtaaaaacag tggagaaggc
     1321 gccaggtaat cctatttttg aatggtctca tttggaatta caacgttact ttgatattga
     1381 tttgacaatt tctgagaaga atgcaccaga aatttggcga cgtgccaacg aaaaaatcca
     1441 aacggattcc tatcgcccaa aagaattgct gaagatgatg gatgtgaaag ccatttgtac
     1501 aacggatgat cctacagatg acttgcgata tcatactgag ctttctaaac aggataattc
     1561 tttcaaggta ttacctactt tccgtccaga taaggtctgg agtatcaaat catcaggatt
     1621 ttcgaactat attttaaaac ttagtcaagc agcgggtata ccaatcaaaa actttaatgg
     1681 gttgaagcag gcattgagcc aacgagtgga t
//
LOCUS       DACDPS010000205         1673 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.213, whole genome shotgun sequence.
ACCESSION   DACDPS010000205 DACDPS010000000
VERSION     DACDPS010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.213"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            27..710
                     /locus_tag="IWJ07_002822"
     CDS             27..710
                     /locus_tag="IWJ07_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP9334335.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            857..1471
                     /locus_tag="IWJ07_002823"
     CDS             857..1471
                     /locus_tag="IWJ07_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAP9334336.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVLALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            1487..>1673
                     /locus_tag="IWJ07_002824"
     CDS             1487..>1673
                     /locus_tag="IWJ07_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334337.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGL"
BASE COUNT          608 a          281 c          349 g          435 t
ORIGIN      
        1 tatataacat tactggagga gatattgtgg atacactgga acaaatcaaa ggcggagtcg
       61 tcgtttcttg tcaagctttg gaaaacgagc cgctacacag ttcttttatt atggggagaa
      121 tggctttggc tgcaaaagaa ggcggagcag ttggtattcg agcaaatacg acagaagata
      181 tcaatgaaat caagcgaatc gttgatttac cggtaatagg gattatcaaa agagattacg
      241 atgggtcaga agtgtttatt actgcgacag aaaaagaagt ggaagaactt ttggagacgc
      301 aagcagaaat cattgcactg gatgcaactt ctcgtaaacg tccgacagaa aaaacaactg
      361 ctgaacttgt ccaaatgatt cacgaacacg gtcggttagc tatggcagat atttcgactt
      421 ttgaggaagc gcttcaagcg caagaagatg gttttgatct tttgtctacg actttagcag
      481 gctatacaga atattcacga aagacgcaga cacctgattt tgatttattg aatcaaatca
      541 tagaacaagt cgatatccca gtaattatgg aaggacatac tgactctcct gaacaagtag
      601 agaaagcata tgaattaggt gctttttcag tcgtagttgg atctattatc acccgtccgc
      661 aattgatcac taaaagatat gtcgaagccg caagtaaagc aaataactaa aaaacacgca
      721 cggcacagat tcttgacaaa aaagaaaaat tgatagatag tttaaagcag attctaagaa
      781 gatgaaaaaa gcattacttt tcattttgtc ttcaagatct gctttttaac gaattttata
      841 gaaagaaggc gaaaccatgt ttgaacggag tccttcagaa ttgacagaac gtgaaaatta
      901 caagttgctg attggttcag tcatccctag accagtagca gtagtaacga ctaaatcagc
      961 aaaagatata gtgaatatcg cacccttcag ttacttcaat atcgtcagtt cgaatcgacc
     1021 catcttatcc ttggcgatcc agagaaaaca agatgcagta aaagacacag caaagaatat
     1081 tcttgagaca agagaagctg ttatccatat ccttgatgag gacaacgtag aagaagtaaa
     1141 taaaacagca gccaatctgc ctaatgagga aagcgaactg tacctgactg acttgacttt
     1201 aactgattca gtaatcattt cagtactagc attgacagaa gcaaaagttc gttttgaaac
     1261 atccttatat gaacatgtcg agatacggaa taaaaataaa gtaacagctg atttattttt
     1321 gctagaagta cagaaatatc atttaaatga aacagtctat gatgataaga caggaaggat
     1381 cgatccaaga aaattgaatg ctgtaagccg tttggcagga aatgattatg cagcaatagg
     1441 agaaattttc acgattgcac gtcccgaata aaaaggagca ctagttatgt taaaaataag
     1501 ctataaacca tcgacagatt caaaagaaat gaaaaaagaa tatgaaacag tgaatgattt
     1561 tctgcaaggg cagtacttag aagttccgcc attgcaagac cattttgtcg ttactactgt
     1621 tactttagat ggtaaggaaa tagaaatgcc agatcaaacg attagcggat tgt
//
LOCUS       DACDPS010000206         1649 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.214, whole genome shotgun sequence.
ACCESSION   DACDPS010000206 DACDPS010000000
VERSION     DACDPS010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.214"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..255)
                     /locus_tag="IWJ07_002825"
     CDS             complement(<1..255)
                     /locus_tag="IWJ07_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334338.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDD"
     gene            complement(257..1315)
                     /locus_tag="IWJ07_002826"
     CDS             complement(257..1315)
                     /locus_tag="IWJ07_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAP9334339.1"
                     /translation="MVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPGLLARQGF
                     IEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQIFYITLSS
                     SRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFSFGTNYAK
                     DFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIWKAALKVI
                     RPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLVSSGIIAF
                     ICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFNKRDVISI
                     VFWYYLGSISYLYLKKEN"
BASE COUNT          652 a          272 c          195 g          530 t
ORIGIN      
        1 atcatccgag aaaaattgat tatggttagt taacaagtta attttacgtt taaaggaaga
       61 accgattttc cggtgattca caaaaacaac atcattaaat agccctaatt cttttatacg
      121 ctcactatat cttctagcgt tttcaaattc agaataaata aataaatctc cttttgattc
      181 tcggtaaaaa ttttgataaa catttaatat attaatcaaa tgtaaaggag tccaagcaat
      241 gaaagctgtc ttcatattaa ttctcctttt ttaaatacaa gtaagaaatg cttcccaaat
      301 aataccagaa aacaattgaa atgacatcgc gcttattgaa aagtacatga gcctccacca
      361 tttcatctat aaaaatagat agtataaaaa ttaaaacaat accaagaaac gcataattca
      421 ctttacttct aaaaaatgtt ttgagtaaac taaagatatt taacacatag aaagcataga
      481 tacatataaa agcaattata ccacttgata caagtacttg aagaaatcca ttatgcatgt
      541 ttccatgtgc tctttttagt tcattcacat ctgtagtatc aagatctact tttttcaaaa
      601 attttgcatc gctattccca tagaaatcgg tctctccata accaaataac aaagttggtc
      661 ttataacttt aagcgcagct ttccaaatcg taaaacgacc agctgaaatt tcggagttat
      721 cactagaatg ctgtaatatt acttcttttt ctggaagtgg agttttatct gattcatttg
      781 actctataac ttctactttt ctatcaatcg atacaggaat tgttgctaaa aaatccttag
      841 cataattagt accaaaagaa aaatatacca atagaactaa taatatgctt accatccatt
      901 tgatttttac aggtagtact tctctaatgc tctttctaaa ttttgaattg gcaaacaaca
      961 aaagaaataa tactaaaaaa gaacaaatta ctacctgggc acctctagac gaagaaagtg
     1021 taatataaaa tatttgtacg ataaaattca tcatataaaa aacacgaatc gtttttgtta
     1081 tttttgaaat taatacacta attaaactta aaataataga agtatatcca aacatagctc
     1141 caacatttgg acttgtatag acaccaaata accgatgttc aataaaacct tgtcttgcaa
     1201 gtaatcctgg cgttcctgtt gggacatgat aagaaatcaa aaaaataaat tgatagatag
     1261 atattagcgc agctaaaaaa attatgataa tgaaaacatt attaaaatac accatttttt
     1321 tctttttttc ctcttcactt tgtgaaaagt ctgcaggaaa aattactatc aaagtaatca
     1381 gcaaatatcc aaaattataa atagatggaa ttaaaaaatt ttctctgtta atgattatag
     1441 ttaagataaa actaatcatt gctccaatca gtagccaaga aaaacgcatt ttaaaaaaat
     1501 ttttctttat aaaaacctca tacaaaacta taattccacc ccaagcaaaa gctattttca
     1561 tcgggatatt agttacatta aaagtactag gcataaaata aagcagacaa atgattaaat
     1621 ttattactcg aaacgcatct cgacttaat
//
LOCUS       DACDPS010000207         1642 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.215, whole genome shotgun sequence.
ACCESSION   DACDPS010000207 DACDPS010000000
VERSION     DACDPS010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.215"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            241..912
                     /locus_tag="IWJ07_002827"
     CDS             241..912
                     /locus_tag="IWJ07_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9334340.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
BASE COUNT          590 a          245 c          218 g          589 t
ORIGIN      
        1 ccgttgtcaa tataaaaagg gaataaactt gcttttttct accatatcag aatgattgta
       61 ttttttattc agaaaaataa aatactcctt ctttttggac tgattttatc gaaacttatt
      121 ttttcttttt ttcatgtatt cttttatttt tataactagt gtattacttc aaaatttgat
      181 attatatggc atgaacagaa ttaaaatatc agttaaaata aaagttaagg agcattgtgt
      241 atgacaatta aaactagtct ccaaaatcaa gcttaccaat ctattcgccg acaaattatc
      301 tatgctgact tagaacctgg aaaaaaagta tcagaaaaaa aattagaaga aacactttct
      361 attggtcgta caccaatacg tgaagcactg attcaactcc gacaacaagg tcttgttgat
      421 acaattcctc aaagcggaaa ttatatctca ttaattgatt tagattcggc caaaaatgct
      481 cgatttgtca gagaacattt ggaacgacaa attatgttag agtgttgtgc caaaatgaat
      541 aaccaaaaaa aacggatttt agaaactata atttcagaac aagaaaaagc tgttagtcaa
      601 aaaaatgctc gtagtttttt tcataatgat aatttattcc ataaagcttg ttttgaaatt
      661 gcagagcgag aagaagtatg gcaatggtta gaaaataaca atacacactt agaacgattt
      721 cgttggctac gagtaaaaac ggaaggatta aagtgggaaa aaattatgga acaacattat
      781 caactatttg aggcattaac aaaacaagat ccagaagaag ctaactttct aacatcgttg
      841 catcttcatt taatgcttaa tgagcaaaat attgttttag ctactcatcc aaaatatttc
      901 aaaaatatat aacttttaac tctttttaaa ttaaatggtt gttctgatca gaaaaatatc
      961 gcttgttttc ttattatgta atatatttca taaactattc tgcataacaa ctattgtttg
     1021 tactttttac attggataac taattatgac tgctgataac ttttgatatt aaactagtat
     1081 ctccaataca aataaaaaat agatatcttg ttcatcaaaa ttagttcgtg gatacttacc
     1141 ttcatttaca tcattcagcg gtttcccatt ttcttttatt ctattatttc attttctaat
     1201 agacttggct tcatttcgca atactttttt tcaataggca tcaaattggt gttgtttaga
     1261 tatttagttg tagttttgtg acatgataag tatgctcctt tttctctttt ccactaatac
     1321 aacgatcaaa gggtgctttt ttcaaaaaac tagaaaaatc aaaataagat tcgtattttg
     1381 attctatata tcctgtttca actacatctt atatttcaca atagaataaa tagccttttg
     1441 catagctgct taacttataa gaaattgcaa tttacttctt caaagtgaaa acgaatatga
     1501 gtaaaataaa attattcttc ttacagataa ttaaaaatag cgcgtaacaa aaatcggcat
     1561 tcaatcacat aattaggatt gatgccgatt acttatattc tacgtatagt tttagtattc
     1621 ttatttttta tcaccagtaa cc
//
LOCUS       DACDPS010000208         1634 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.216, whole genome shotgun sequence.
ACCESSION   DACDPS010000208 DACDPS010000000
VERSION     DACDPS010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.216"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..547
                     /locus_tag="IWJ07_002828"
     CDS             <1..547
                     /locus_tag="IWJ07_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334341.1"
                     /translation="KIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYGI
                     DETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTLL
                     ISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLSE
                     EKILLPENWAELRKLVEYGII"
     gene            534..>1634
                     /locus_tag="IWJ07_002829"
     CDS             534..>1634
                     /locus_tag="IWJ07_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP9334342.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAARGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVA"
BASE COUNT          538 a          212 c          262 g          622 t
ORIGIN      
        1 aaaaattccc aaaatagatg aaaacagtaa attgatgaaa aacatacaat caatctttgg
       61 gatagaagaa gtttttgtcg gagataaatg tatctttttt gatcaacctt tattatctga
      121 tggttatggc atagatgaaa cagatatttt cgctaaagta aatagtatat tttctggaaa
      181 aaaagaaact ttaattaagc ttcatcctag agcaaggaca aatcgttatg gtgaaaaaaa
      241 catattgaat tccagtttac cgtttgaagt cttgctactt gaaaatgact tctcttcaac
      301 acttttgatt agtcctatat ctacaatttc tttttcacca tctatgatgt ttgaaaagac
      361 gatcaactct attatgttgg ctaaattaat tttaaatgaa tatacagcat tatctgaaga
      421 taaaaaagaa acattgacaa atattgttct tttctgtgaa cattataacc gtctatcaga
      481 agaaaaaatt ttactcccag aaaactgggc agaattaaga aagttggtag agtatgggat
      541 tatttaacac actttccaag tcaaaattag ttaagtccag tttctggtat actataggga
      601 atttttttat taagggaatt tcattcataa ctattcctat ttttgtaaat attactactc
      661 aagcagaata tggtttgatt aataatttca cttcatttgt atcaattttt tcgattcttg
      721 ttgggctaag cctaaattcg gcaattaata atgcaagttt tgactataaa gatgacataa
      781 atgaatttat gtcatcggta ctttctctag ctacactttc gttaattgtg ttcttattaa
      841 tagggaacat atattttttg ttttcaaatg ctttttttga tatgaaccaa cttgtattta
      901 atttgatgtt attacaaagt tttgctagtt ttgtaattag ttatatccag tcttatttta
      961 cgattaactt taaccatttt aaatatttga ttttatcttt tatatctact tttttgaata
     1021 ttgcaatctc tgtagctttg atgcagacat tatattttga tgataaatat gttggcagag
     1081 tagtcggcgg aacactgtca tttgtgttga taggtttagt aatttattta agaataatga
     1141 taaagggtgc tcattttttt gataaaaaac agtggagata cgctttaaaa atctctttgc
     1201 cgctaatccc acattcctta gcaaatatag ttttagcaca attcgataga attatgatta
     1261 attcttattt aggtcctgct gcgaggggaa tatattccta cattagtaac ataggtatta
     1321 ttctaagcgt aatatggatg tctactaata acgcctgggt tccatggttc tatggtgaga
     1381 tgagtaaaaa taatgaaaaa aaagtgaaga aagttagtaa ctattattta gtagtcttta
     1441 ctttgattac gatgattgta atggtagttt cgattgattt agcaaaattt atggctccga
     1501 aagactatta ttctgggtta tctttagtgg ttccgataac cttaggctat ttctttcagt
     1561 ttttatatag tttaccagtt aatgctgaat tttatgagaa aaaaacatcg tttattgcga
     1621 ttggtacagt agca
//
LOCUS       DACDPS010000209         1627 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.217, whole genome shotgun sequence.
ACCESSION   DACDPS010000209 DACDPS010000000
VERSION     DACDPS010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.217"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..335
                     /locus_tag="IWJ07_002830"
     CDS             <1..335
                     /locus_tag="IWJ07_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP9334343.1"
                     /translation="EITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQT
                     SLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHSTL
                     TKVGEINE"
     gene            328..1230
                     /locus_tag="IWJ07_002831"
     CDS             328..1230
                     /locus_tag="IWJ07_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP9334344.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            1263..1613
                     /locus_tag="IWJ07_002832"
     CDS             1263..1613
                     /locus_tag="IWJ07_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP9334345.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
BASE COUNT          594 a          284 c          303 g          446 t
ORIGIN      
        1 aagaaattac agaaaaaact atccattacc tcagtgaaaa tggcgatcga atcatctatc
       61 acgaccaaga tgctttgaat gctgtacttt atgaagattg ggaacaactt catccaaaat
      121 ggaacatgca gacttcgctg atttttgaac gtcatccagc accaaatgaa aaatatgaac
      181 gtcagtataa agaaggaaat gaaaaacctt cgatcgttca ctttacagga catgataaac
      241 cttggaatac gttgaaagat catccgtata ccaatttata cttaaaaaaa ttagcacaca
      301 gtactctaac gaaagtaggc gagatcaatg aataaaaaag aaatagcagt agtcgcaagt
      361 tgtaacacaa aattcgtacc acatttagct gcattatttg tctcagtatt agataattgc
      421 aacccgtcta aatttgttcg gttctatgtg attgacgatg atatcgattt tgaaagtaaa
      481 cagcttttac gcttctcagt gaaaaacgca cgaatgaact cagatgtgga attcttgaaa
      541 atcaataaag aattctttac aaatgtggta atcagcgatc gtattccaga aacagcttat
      601 tatcgtattg cgattccgga attgttccgc ggcacagaag ttgaacgtat cttatatatg
      661 gattgcgaca tgatcgcttt acaagatatc tctaaattat ggcgtcttga cttcggtgat
      721 tcaatcgtag cagctgttga agacgctggg ttccatcaac gactggaaaa aatgaaaatt
      781 tcagctaaat caatgcgtta tttcaactca ggtttgatgc tgatcaatgt gaaaaaatgg
      841 ttagatgaaa atattacgca aaaagtatta gatttcatcg aacataatcc agaaaaatta
      901 cgattccatg accaagatgc attaaatgct atcttacatg accgttggtt accacttcat
      961 ccgcgttgga atgcacaagg ctatatcatg gcaaaagcaa aaaaacatcc aacagcagca
     1021 ggtgaacgtg aatacgaaga aactcgcaac aatccttaca ttatccattt ttcaggacat
     1081 gtgaaaccgt ggagcaagga ttttgaaggg ccaacgaaga aatattacga aaaatatgca
     1141 gggatgacgg ctttccgttg tgtagctaaa ttccctaaat atccaaaata tgcaaaagtt
     1201 caaccaaaaa cagatgtagt tgttaaataa tagaagacaa caactgaaga gggggagtag
     1261 gaatggaaat tacaatgtct aaaaatgcag tagagacgtt aattgaaaag gttggggaaa
     1321 atacaaaaat cgcactcgcg cttatcaatg attccgatcc atttctaagg gacaaaggtg
     1381 cttttgcaaa aggcagcttt tttcaaatca ttccttttgt ttcggaattt ggagaatatg
     1441 cgactaaaat cgagcatcca ttattggaca tctatacatc taaattagag caaaattatt
     1501 tcggtaaacg attgaacatg gacttcaaca aacagcttga cagcttttca ttagaaaatg
     1561 aaatcgcagt actcgattat aatatcaaat taaagaactg cttcttcagc taatagtttc
     1621 gagaaag
//
LOCUS       DACDPS010000210         1609 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.218, whole genome shotgun sequence.
ACCESSION   DACDPS010000210 DACDPS010000000
VERSION     DACDPS010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.218"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            99..1565
                     /locus_tag="IWJ07_002833"
     CDS             99..1565
                     /locus_tag="IWJ07_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAP9334346.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          450 a          292 c          334 g          533 t
ORIGIN      
        1 gaaaattagt ttatatttct taaataagtt ttttagttaa atttttcggt aaagatgtat
       61 aatattttct gaaaatttaa agaattgggg tatagattat ggaaaaacag caagacaagg
      121 gcttcttcgg gcagccgaag ggattgtcaa ctttgttctt cactgagatg tgggaacgat
      181 ttagttatta cggtatgcgt gcattactta tctattatat gtacgataca gttgccaacg
      241 ggggacttgg attaccacga gcaacagcat tagctatcat gtcgatctac ggatctttga
      301 tctacatgtc tagtatcgtc ggcggatggg tatctgaccg agtgttagga gcaaaacgca
      361 ctgtattctt cggtggagtt ttcattatgc ttggccacat cgtcttggct ttaccatttg
      421 gtgtaagtac attgtttgtt tcgatggcat tgattattat cggaaccgcc tttttgaaac
      481 caaatgtttc tggcatggtc ggacatcttt attcaaagac tgacttgcga agagatgcag
      541 gattttcgat tttttacatg ggtgtcaact taggggcatt attagcacca ctaatcgttg
      601 gtacaatcgg tcaagaatat aactatcatt tagggttctc tttagctgct attgggatgt
      661 tctttggatt attacaatac atcatccaag gacgcaagac attagacgga gtgggtacaa
      721 caccgccgaa tcctttgaca actgaagaac gtaaaggatt catccgtaac ttgatcatcg
      781 cacttgttgt tatcgctgca gtattcggcg gggcacaact aactggtcat ctgacaattg
      841 acttttttgt caatacaatc agtgtattgg ggattatctt accgttgtat tactttatca
      901 aaatgctgac ttcaaaagat gtaactgctg aagaaaaacc aagagtatgg gcttatatcc
      961 cattattcct tgcagcaatc attttctggt cattacaaga acaaggttca tctatcttag
     1021 ctttatttgc tagtgaacgt acacaatcta cgctatttgg tgtaacgatc caagcaagct
     1081 ggtatcaatc attgaatcca attttcatct ttattctgac accagtattc gtaacgatgt
     1141 ggacgaaact tggtgaacgt caaccatcta cagtagtgaa attctctctt ggacttttat
     1201 ttgcaggatt atcgtacttg ctattgatgt tgccgggtat gctttatggt acaagtggaa
     1261 aagtaagtcc attgtggttg ttgggaagtt tcttcatcgt ggttattgct gaattgtgtc
     1321 tttcaccagt tggtttgtct gtgacaacaa aattagcacc acgagcattt gaatcacaaa
     1381 caatcgctat ttggttctta gctgatgctt cttcacaagc aatcaatgca caaattgcac
     1441 gcttctacac accgggaaca gaatctgctt acttcggtat tatcggtgca gtagcaatcg
     1501 tcggtggtat cttgctattt actgtgaaag aaccaatcaa aaaactaatg ggctctattc
     1561 attaatcaat gaacataaaa acaggaattg cttatttgca gcagttcct
//
LOCUS       DACDPS010000211         1578 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.219, whole genome shotgun sequence.
ACCESSION   DACDPS010000211 DACDPS010000000
VERSION     DACDPS010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.219"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            78..193
                     /gene="rrf"
                     /locus_tag="IWJ07_002834"
     rRNA            78..193
                     /gene="rrf"
                     /locus_tag="IWJ07_002834"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            203..276
                     /locus_tag="IWJ07_002835"
     tRNA            203..276
                     /locus_tag="IWJ07_002835"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:237..239,aa:Asn,seq:gtt)
     gene            316..405
                     /locus_tag="IWJ07_002836"
     tRNA            316..405
                     /locus_tag="IWJ07_002836"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:350..352,aa:Ser,seq:gga)
     gene            420..491
                     /locus_tag="IWJ07_002837"
     tRNA            420..491
                     /locus_tag="IWJ07_002837"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:453..455,aa:Glu,seq:ttc)
     gene            507..579
                     /locus_tag="IWJ07_002838"
     tRNA            507..579
                     /locus_tag="IWJ07_002838"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:540..542,aa:Val,seq:tac)
     gene            589..661
                     /locus_tag="IWJ07_002839"
     tRNA            589..661
                     /locus_tag="IWJ07_002839"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:622..624,aa:Asp,seq:gtc)
     gene            697..772
                     /locus_tag="IWJ07_002840"
     tRNA            697..772
                     /locus_tag="IWJ07_002840"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:730..732,aa:Phe,seq:gaa)
     gene            774..854
                     /locus_tag="IWJ07_002841"
     tRNA            774..854
                     /locus_tag="IWJ07_002841"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:808..810,aa:Tyr,seq:gta)
     gene            861..934
                     /locus_tag="IWJ07_002842"
     tRNA            861..934
                     /locus_tag="IWJ07_002842"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:893..895,aa:Trp,seq:cca)
     gene            937..1009
                     /locus_tag="IWJ07_002843"
     tRNA            937..1009
                     /locus_tag="IWJ07_002843"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:970..972,aa:His,seq:gtg)
     gene            1021..1092
                     /locus_tag="IWJ07_002844"
     tRNA            1021..1092
                     /locus_tag="IWJ07_002844"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1053..1055,aa:Gln,seq:ttg)
     gene            1138..1208
                     /locus_tag="IWJ07_002845"
     tRNA            1138..1208
                     /locus_tag="IWJ07_002845"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1170..1172,aa:Cys,seq:gca)
     gene            1229..1313
                     /locus_tag="IWJ07_002846"
     tRNA            1229..1313
                     /locus_tag="IWJ07_002846"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1263..1265,aa:Leu,seq:caa)
     gene            1375..1447
                     /locus_tag="IWJ07_002847"
     tRNA            1375..1447
                     /locus_tag="IWJ07_002847"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1408..1410,aa:His,seq:gtg)
     gene            1457..1528
                     /locus_tag="IWJ07_002848"
     tRNA            1457..1528
                     /locus_tag="IWJ07_002848"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1489..1491,aa:Gln,seq:ttg)
BASE COUNT          370 a          355 c          415 g          438 t
ORIGIN      
        1 actcggaaaa aaacttatgt gacttcaaat ccagttttga gtgaacaaaa tttactcaat
       61 aaaagataac accacagtgt ggtggcgata gcgagaagga tacacctgta accatgccga
      121 acacagaagt taagcttctt agcgccgatt gtagtgaggg gttgcccctt gtgagagtag
      181 gacgtcgcca cgctgatgtt attccggcat agctcagttg gtagtagcgc atgactgtta
      241 atcatgatgt cgtaggttcg agtcctactg ccggagttct cattttgaga aagagtattg
      301 agcttctttc actctggaga gttgtccgag aggccgaagg agcatgattg gaaatcatgt
      361 agatggtgcc aaactgtctc aagggttcga atcccttact ctccgtagca aatttacctg
      421 gcccgttggt caagcggtta agacaccgcc ctttcacggc ggtaacacgg gttcgaatcc
      481 cgtacgggtc attttaattg aatgttggag gtttagctca gctgggagag catctgcctt
      541 acaagcagag ggtcagcggt tcgatcccgt taacctccat aatgaactgg ttccgtggtg
      601 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
      661 gtcgttcttg ttattcaatt aataatttta aaatatggcg cggtagctca gttggtagag
      721 caacggattg aagctccgtg tgtcggcagt tcgattctgt cccgcgccac catggaggag
      781 tagcgaagtg gctaaacgcg acggactgta aatccgttcc ttagggttca gtggttcgaa
      841 tccactctcc tccattttat aggggcatag tttaaaggta gaacagcggt ctccaaaacc
      901 gttagtgtgg gttcaattcc tgctgcccct gccatggcga atgtggcgaa gtggttaacg
      961 caccggattg tggctccggc attcgtgggt tcgattccca tcattcgccc tttttattct
     1021 tggggtatag ccaagcggta aggcaaggga ctttgactcc ctcatgcgtt ggttcgaatc
     1081 cagctacccc agtttctata atttattctt tattttatag attgataata gtataatggc
     1141 ggtatagcca agtggtaagg cagaggtctg caaaaccttc atcaccggtt caaatccggt
     1201 taccgcctta gtactaataa atcaacttgc cggcgtggcg gaattggcag acgcgctgga
     1261 ctcaaaatcc agtgcccgtt gagggcgtgc cggttcgacc ccggccgccg gtattgacta
     1321 agatgtcttc atcttagttt tttattgaga aattaaacaa catcttttat gatggcgaat
     1381 gtggcgaagt ggttaacgca ccggattgtg gctccggcat tcgtgggttc gattcccatc
     1441 attcgccctt tattcttggg gtatagccaa gcggtaaggc aagggacttt gactccctca
     1501 tgcgttggtt cgaatccagc taccccagtt attattttta aatttgccgg cgtggcggaa
     1561 ttggcagacg cgctggac
//
LOCUS       DACDPS010000212         1574 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.220, whole genome shotgun sequence.
ACCESSION   DACDPS010000212 DACDPS010000000
VERSION     DACDPS010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.220"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            32..>1574
                     /locus_tag="IWJ07_002849"
     CDS             32..>1574
                     /locus_tag="IWJ07_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP9334347.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLIIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKK"
BASE COUNT          548 a          217 c          329 g          480 t
ORIGIN      
        1 aaactaacat atcagtttga ggaggttaat tatggtttcg attaaaaatt ggcaaaattc
       61 tatcgaagaa ttcaatgaaa tgcagattct actgccgtgt tttgatgtca aagaagttaa
      121 gcaagaaggc cagcaacatc cagtatggct tcattttggt ggaggaaatc tttatcgagg
      181 atttcatgca gaaattgctc aatgcttagc agatcaaaat gaattagaaa caggagtagt
      241 tgtgtgtgaa acatttgatg aagaagtagt ggaaaaagct tatcatgact ataacaatga
      301 cattttggaa attgttatga gtggagatgg tgaactcaaa aaaagacttt tggcagtaac
      361 ggctgatagt ttatattgca atccgaagaa tatagcggac tatcaaagag tggttaaata
      421 ttttgaagat cctaaattac agtttacaac gtttactatt actgaaaaag gttacagttt
      481 aaaagattct aaagggcaat tcttttcggt agtcaaagct gatatcaaat ctggacctag
      541 caatcctcaa catacgatga gtattgtaac atccttgtta tatagcagat ttaatgctgg
      601 agagttgccg attgcgatgg tttctactga taatttctca cagaatggtc aacgttttca
      661 agatagtatt tttacgattg cagaagcttg gcaaaaagaa ggatatgttt ctgaaaaatt
      721 tattgcttat ttaaaggata aagaaaaagt gacttttcct tggagtatga ttgatcgaat
      781 tacgccaaat ccttcagaga atgtaaagga agatctattg atgatggggc tcgaggattt
      841 agatatcatt cacacaaaaa aacataccaa tattgctgct ttcactaata ctgaggtagt
      901 tcattattta attattgaag atagttttcc aaatggccgg cctaaattgg aaaaggctgg
      961 tgtgattatg acagatagag agacggttga taaagcagac atcatgaagg ttacgacaag
     1021 cttgaatcca cttcatactg ctttggcagt aacaggttgt ctgctaggat atcaatcaat
     1081 ttcagcagag atgcaggatg atgatttggt tggtttaatc aaaggaatag gttatttaga
     1141 aggtttacca gtagttgaaa atccaggcat tattgatcca aaacaattta ttgatgaagt
     1201 aattaataaa cgtttgccta atccctatat tccagataca ccacaacgaa ttgcaacaga
     1261 tacttctcaa aaagtagcga ttcgctatgg tgaaacaatt aaaaagtatg tcgaaaaaga
     1321 cggaaatgca gaaaatctaa catttattcc tatcgctatt gctgcttggt tacgttattt
     1381 actggcagtc gatgatcaag gggaaaattt tgtgccaagt cctgatccgt tattggtaga
     1441 gttgcaggat aaattatcag tattgaaatt aggtgaacaa gatccttcaa agattcattg
     1501 tgcggtacag gaaattttag aaaataaaga aatctttggt ttagatttat atcaagttgg
     1561 ggtaggaaag aaaa
//
LOCUS       DACDPS010000213         1564 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.221, whole genome shotgun sequence.
ACCESSION   DACDPS010000213 DACDPS010000000
VERSION     DACDPS010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.221"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(157..456)
                     /locus_tag="IWJ07_002850"
     CDS             complement(157..456)
                     /locus_tag="IWJ07_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP9334348.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            807..1439
                     /locus_tag="IWJ07_002851"
     CDS             807..1439
                     /locus_tag="IWJ07_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP9334349.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
BASE COUNT          540 a          245 c          216 g          563 t
ORIGIN      
        1 tgtttttgtg ttaattaaag gataaaatac aatataaaga aacatctgtt tctttattta
       61 gaataaaaaa atacagactc tgttcaaata aatgaaccta gcctgcatta tgagattgac
      121 tatacttctt aattcattga gatgaataac tcgtttttat aaatgcccaa aagtaatcgg
      181 tgccgaagcc attcctcgac ctaattcttt ttcaaatttt cctattgttg atatctccat
      241 ataaaacggt gatactggtt tagacaattc tcccctgatt gctaacggac gtaaactacg
      301 ttccaaatta tttatgactt taggataata ctgacctttt tctaagcctg tctttgcgtc
      361 tatcaaaagt atacgttcct tttctttgat ctctggattt aaaacgagat catataattt
      421 tagtaccatg ttttctattt cttcactttt agacatcacg atttcctccc aaaaatatat
      481 ttcttcccac tacattctat aaacaaagta tacgctacat ctagattttt ttaaatcatt
      541 actggattgt taaaaccttt caatacactg ttatggcaac gatttttatt ttaaaaacat
      601 tctaaattaa atcagtaaaa atttttattc taagattttt attagaatta cgcctaattt
      661 gtgtctctca gggcaattct aagaacgttt caagagatag cgacactatc gttatcgaaa
      721 aaggcgaaaa cctatctcat tttctgctaa tgtaagttgg tttagctttt gttaatatga
      781 ctttatgaaa atggaggtaa ttaaatatga tttttcctaa agttaatgaa gcaagcttga
      841 ataaaggatc cttttttcat atctatgaaa acgaagattt tgaactgtat aatatccttt
      901 cacaaaaaga ggtcatcgaa aaccaaataa tctgccatcc aattatttac aaatctattt
      961 tcaagtatgc attttttgct tccaatgatt ttcaaagaat ggagattacc gactgtatat
     1021 tcgaaaactg tgatttttca ggctgtatat tgacaaaaag catgttgcat cgcgtgaaat
     1081 ttgtcaattg taaattaaca ggtactaaat tgatggaaag ttatattggg aacacgttat
     1141 tcgagaattg taaaatggac tatgtaaatt tatctggttc aaacataaaa gaaagtacct
     1201 ttgagcactc tgtcttatca agcgcagatt ttgtagactg ttccctcact aaaataatgt
     1261 tttctactaa tgatttagaa aatgctagct tttttgatac tgatttaaaa aatatcgatt
     1321 taagtagcaa ctcctttgaa atgattgaag tacgctcgga acatctaaaa aattgtacgc
     1381 ttaatcagtt gcaagcactt ggttttgcta gaaaattttt acaaataaaa gtagtataat
     1441 caaaatgaat atataagata aatttttagc aatacaaata tccataaaaa aacattgcct
     1501 attttaactc ttcatgtata ctgactctat cttcaaaaac tgaatagtag gagttattat
     1561 catg
//
LOCUS       DACDPS010000214         1562 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.222, whole genome shotgun sequence.
ACCESSION   DACDPS010000214 DACDPS010000000
VERSION     DACDPS010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.222"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(594..938)
                     /locus_tag="IWJ07_002852"
     CDS             complement(594..938)
                     /locus_tag="IWJ07_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334350.1"
                     /translation="MISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFHYSPYRLF
                     QYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHDEVYLAEC
                     LANLEQKGIYLT"
     gene            complement(1035..1280)
                     /locus_tag="IWJ07_002853"
     CDS             complement(1035..1280)
                     /locus_tag="IWJ07_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9334351.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1350..>1562)
                     /locus_tag="IWJ07_002854"
     CDS             complement(1350..>1562)
                     /locus_tag="IWJ07_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334352.1"
                     /translation="ALVASIVYLMNWNKERFYLAILTLPAIYFMWKVFNYNYFEPDSV
                     FIEELSGLVLSLLIVILYLIRLNKKH"
BASE COUNT          553 a          267 c          283 g          459 t
ORIGIN      
        1 taagcttttt taaagaatat ttagaaatta ttaattttcg tctgaaaaca aaaaaagaaa
       61 gcgttattat tttagtctat tttcaggggg ggaaccttat gataaaaaaa ctatttttac
      121 tatcaatatt aacgctagca atggcaggtt gttctgactc tggcttagca actgaactga
      181 tcacaaaatc aactgaagcc acgacagagg agttaaaatg tggaaacgat cacagtgact
      241 acagaggctt aaatcgacga tgcatgggaa tattaggcag aagcaattgt aagcatcgat
      301 gtagtaatcg aagaaggtgt agaagaagca acagaaaaat caaaagaaag atcaaaagaa
      361 gcaatctgtg ctgtgcatcg tatacagacg tcgaagatgc ggtactttta aaccacgtca
      421 ggagatgttc atttatgtct agggaagttg gcgatgaacc aaaactagag tacgctttta
      481 gttttgcacg tgaggatgaa gataacccta aaacttacga cattttagct tgatctaaga
      541 tggttaaagc tttttaatga agtaagagga gatagaaggc ttttctagtt atattaggta
      601 agataaattc ctttttgctc caaatttgct aaacattctg ctagataaac ttcatcgtgt
      661 tccggataaa tcggcacgtt gcgctctaca gtaggtaaat caaaaaatgc ttcgcatgat
      721 ttgccacgat acataggatt tttccactct ggtgctgggc tgtaaccacg tttttccatt
      781 tcatccatga caagctgatg atattggaat aatctataag gagaatagtg aaaaacataa
      841 tccacagtag catgtttctt tccccagcca tttcctctta atgcacagca ttcccgatgt
      901 tgtcctaaaa gctgttgacg aggaagcttt gaaatcaatt tttcatgcca taatcacata
      961 gaaatgtctc ctttatatat agattgttaa ccttagacgt taaatgaaaa aaccagcacc
     1021 aaggctggaa aaaatcactt cagtttgctt gctgtttctg agcgaccaat taggtaatca
     1081 acggaaatat cgtaaaagtc agcgatatag attaattcaa tgatgcttgg aatttcttct
     1141 ccttcttcgt acttacgata tttgaataaa ggaatactta acgcgctaga aacagctact
     1201 tgagataaag atcggccagt tcttaagaaa ttcagacgac aacgtaaagt atccagtaaa
     1261 atagcttctt cgatactcat ttaatcacct ttcattatag tagaatagtt ataatgacta
     1321 tagaaagcta cacatgcaat caatagtact caatgttttt tatttaaacg tatcaaataa
     1381 agaatgacga tcaataaaga caaaaccagt cctgataatt cttctataaa aacactatcg
     1441 ggttcaaagt agttgtaatt aaaaactttc cacataaagt agatagccgg aagagtcaaa
     1501 atagctaaat aaaaccgttc tttgttccaa ttcataagat aaacaatact tgcgaccagt
     1561 gc
//
LOCUS       DACDPS010000215         1551 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.223, whole genome shotgun sequence.
ACCESSION   DACDPS010000215 DACDPS010000000
VERSION     DACDPS010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.223"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(368..1486)
                     /locus_tag="IWJ07_002855"
     CDS             complement(368..1486)
                     /locus_tag="IWJ07_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334353.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
BASE COUNT          441 a          343 c          252 g          515 t
ORIGIN      
        1 tttttaaacg gtgttccaga agtagcttct tcggaaataa gccgaaattt cacaaaaatt
       61 tgagaaataa tttttgtcaa tctcttctta tttcttgaag ctgaccactt ctgtcacaac
      121 ctcttcctta aacggtgttc aaaagcttgc atctgcggta ataagcccac caaacaaaaa
      181 atatgaagag ctatttttgc ttgctgttct tattacttga tgcagaacag ctttttcaca
      241 acctctcata cacggtgttt aatcagctga ccttcggtat tagctcaaaa aacggaaaaa
      301 tatgtgaaac tatttttccg tttgtttctc taatactcag gtcaaaacac tgatttcaca
      361 acctctttta ataacgaaga atcgctgtca gactcttttc atctggcgca tctttttgat
      421 ccaaaataaa cactttcccg ccattaatga tcacatcatc tgctaatgca ttcaacactt
      481 gacgccgatc atattctgtt tcaggatctt caccgaaacc atcagctaag tttgacgtag
      541 cgataaataa atgtgacact tttccttctt tcgcagctgg aacgatatca actaactgat
      601 cgatgaattt cttatctaag agtttattgt attcttttga ttcttgagct gcgagttcac
      661 ttgacagctt cttcgcccct tcttcgatat ctctaaagga tagctgcgca ggagaagcag
      721 aaatcgcaac gtctgcacaa taatagggat ttttagcaat ttttttgaac atcgtctgat
      781 tttctggtaa tgcaaataaa taaatcggcc attgttcagg attttcgaaa gaatccttca
      841 agtacgtatc aacagcttgg taatagttga cccaatcgat ctctacttct tcatcttttg
      901 gatttacgcc atggaaagtt actccatctc gggctccgcc tcctccatta gaagagaagt
      961 tcaaaccgcc acctgtcagt tcagatccca atgcagtaac gacatcaacc ggtgcccctt
     1021 ctggtagaga tacttctttt acttgtttgt tgctgacctc atacaatttc atggagtcgc
     1081 ggttcaatcc caataaataa taagaatagt tgaactgccc atttttgata atagccagta
     1141 aataaggaag atcacttaca tagtattgat cgtctaccgg gatactcaaa cgatggatat
     1201 aggtctggtc agcagttata atgaccgcta cactcgtagt tgcactacgc caaaacgaag
     1261 gatctcctaa caaattttca attttttctt ggaacgggga ccagtcatgt tcggaatatt
     1321 ttttctcaaa acgtttcttt gcttctttcg caaaattttt gaatttgatc tgatcttttt
     1381 ccacatcttg atgggcgaca tgcgtattca gcatgatcgt gacgaatggt cctttagcgc
     1441 cttctgaagt caattgtgtc aataattctt tatccttttt tgccataaac gtacccctcc
     1501 taaaaaaatc attcctttgt ttctatctaa agtctaccac aacactattt t
//
LOCUS       DACDPS010000216         1534 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.224, whole genome shotgun sequence.
ACCESSION   DACDPS010000216 DACDPS010000000
VERSION     DACDPS010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.224"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..532
                     /locus_tag="IWJ07_002856"
     CDS             <1..532
                     /locus_tag="IWJ07_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HAP9334354.1"
                     /translation="AFFYAQEAKISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQN
                     HSFDQILNMLEDPSLSYLKLALTDKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTG
                     ITKAHSLVFLADYLGIESSDIMAFGDQENDIDMLNYSGLSIAMGNAQRQVKEIANQVT
                     LTNEEAGVAYILKNFY"
     gene            572..1069
                     /locus_tag="IWJ07_002857"
     CDS             572..1069
                     /locus_tag="IWJ07_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9334355.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLFPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(1454..1527)
                     /locus_tag="IWJ07_002858"
     tRNA            complement(1454..1527)
                     /locus_tag="IWJ07_002858"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1491..1493),aa:Ile,seq:cat)
BASE COUNT          526 a          286 c          280 g          442 t
ORIGIN      
        1 agccttcttc tatgctcaag aagcgaaaat atccatcatt gctagccgag aaacccaaga
       61 tgatcagctg acattcgatg aatcaaatct agtcaaagat gacccttatt atgaacattt
      121 caatttacag aaccattctt ttgatcaaat tttaaatatg ttggaagatc cttctcttag
      181 ctatcttaaa ttagcattaa cagataagag tgaagaaaaa ctacagaagc tacaaagaga
      241 tctgcaagca gatggtatcc aatcagtatt ctctgatccg catttcttag aaatcacgcc
      301 tacaggtatc actaaagctc attcacttgt ttttctggcg gattaccttg gaatcgaatc
      361 gagtgacatc atggcttttg gcgatcaaga aaacgacatt gatatgttga attacagcgg
      421 gttgagtatt gcgatgggga atgcacagcg acaagtaaaa gaaattgcaa accaagtaac
      481 actgacgaat gaagaagctg gtgttgctta tattttaaaa aacttctatt aaaaagaata
      541 aaatgacgat atgaatgtag gaggaaagaa aatgttagtt attattgata tgcaaaatca
      601 tattcttgat ccaaacagcg aattttatat tccagagtca gatcaattgg cagaacgtat
      661 tgctcaaaga ttagctaaag cacgccaaaa taatgaatat attctcttca cacgggatat
      721 accagttgaa ctgaaggaca aagaagaaga acgagaagat ttgcaattga tccctcttct
      781 ttttccacaa gaaaatgaac gagtgattaa aaagtattac tttacgattc cacctgaaac
      841 tttagctgaa atccaggaaa ctttatttga aagcaaagaa gaacaaaaag agatcgaagt
      901 agtcgggatt gaaacaaact tgtgtgtact ctctaatctg ataggcctgc agagtgcttt
      961 tcctgaagca gacttttttg ttgatccaat gttagtctcc agtagaaaac acggagaatc
     1021 cgctttagag ctgttgaaag atttcaacgt ctctatcgtc gaatcataaa aatatctatc
     1081 ttcatttacg atatttaaca cacaataaaa aaagcacgca gaactgtttt agtcctacgt
     1141 gctttttcta tcaaagtaga ttggcttttt tatgacacag agtgaaacgg caggctcacg
     1201 acctctttct taaatggtgt tcaatcagct gaccctcggt attagcttaa caaacggcaa
     1261 aatatgagga actatttttc cgtttgttaa gctaatactc aggtcaaaac gctgatttca
     1321 caacctcttc cttaaacggt attcaaaagc ttgcatctgc agtaataaga acagccaagt
     1381 aaaaacataa gaagctgttt ttacttggct gttcttatta cttgatgcag ggcagctttt
     1441 tcacaacctc ttatgtacct tgtaggactc gaacctacga ccggacggtt atgagccgtc
     1501 tgctctaacc aactgagcta aaggtacatc ataa
//
LOCUS       DACDPS010000217         1524 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.225, whole genome shotgun sequence.
ACCESSION   DACDPS010000217 DACDPS010000000
VERSION     DACDPS010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.225"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..223
                     /locus_tag="IWJ07_002859"
     CDS             <1..223
                     /locus_tag="IWJ07_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAP9334356.1"
                     /translation="KVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSLNGI
                     CLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            242..1492
                     /locus_tag="IWJ07_002860"
     CDS             242..1492
                     /locus_tag="IWJ07_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9334357.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT          488 a          237 c          267 g          532 t
ORIGIN      
        1 aaaagttcta gtactaacgg ctaatggaac aagccaagac tcttctgacc aagaaactat
       61 taaaaaaatt gcattagcat caaaagcatg tggagctgat attcaacaca taggggattc
      121 tttaaatggt atttgtcttc cagaaaatat ttttgctttg ggtcaagcaa ttcggggata
      181 tcgtcatcaa attattatgt taggaaaatc taattatcgt taattaagga ggaaatgaaa
      241 gatgatcgac aaagttcaaa aaatagctgc aaaaattagt aatcaaagac atctactagc
      301 tttaagagac agcatggctg cagttatgcc attaatcatt attggttcct tttttatgtt
      361 attagcaaat ttcccgatag aaggatttac aaaatggtta tctgaaattg gagtacaatc
      421 ctttttgaac aaagcatcag attcaacatt tggtattatt ggattagctg ttacctactc
      481 tttgggacac aatctagcaa agacttatgg tgtagactca ttatcagcag gattattatc
      541 tttgagtgga tatgttttac ttacaccttt attggatgca gatggttcta taggatttcc
      601 aacaaaatac tttggtacct cgggtctttt tgttggtatt attgtagcac ttgtatctac
      661 agaaattttc cggtggtttg tacttaaaaa tattacaatt aaaatgccgg caagcgttcc
      721 accaaatgta agccgagctt tcgttgctat tattcctggc ttttttgtcg ttctactatg
      781 gtttattgtt gttgtaattt gttataaact tggaattgaa aatgttcatg ctcttattgc
      841 agatacactc gcaaaaccac tatctttact gacgaaaact ttaccaggaa ttatattggt
      901 tatacttatt caatgtttct tttggatgtt tggtattcat ggtgcacagg taacggggcc
      961 aataattgaa ccgttattat tgcaaaactc tgatgtaaat agaattgctt atcaagctgg
     1021 aaaagaactt cctaacatta taacttatga attcttatat aactttgttt tttctggtgg
     1081 tgcaggttgt ttatttgcac ttgccatttt aacatttttc ttttcaaaga gtcaggaaaa
     1141 taaaacttta ggtaagttat caatcgctcc agtaagtttt caagtagcag agccccttct
     1201 atttgggttt ccaactatat taaatataaa aatgttaatt ccatttgtaa ctgcaccagt
     1261 agtaacaaca ttaattactt atttttcaat gagtatggga ttagttgcaa aacctgtagg
     1321 agcaacaatc ccatggacaa ctccaccgat aattgccgga tttttagctt ctggtggacg
     1381 aataagtggt gcagtgattc aagtaataac aattgcaata aatgttttga tatactatcc
     1441 attctttaaa ttagatgata atgccaagct aaaaagcgaa aaaaatgatt agaactacct
     1501 atatgtttct aactgtagtt aagt
//
LOCUS       DACDPS010000218         1524 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.226, whole genome shotgun sequence.
ACCESSION   DACDPS010000218 DACDPS010000000
VERSION     DACDPS010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.226"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            444..1256
                     /locus_tag="IWJ07_002861"
     CDS             444..1256
                     /locus_tag="IWJ07_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334358.1"
                     /translation="MILLAVSMSLLIYTHILSTFMSVIVIGFLFIGSLFSKRKERKEM
                     SNLISNMIKAVGWTILLTAYVWYPMLQQVLHQKINRPFRTKLQERALNISDSLIGAVN
                     NDLVTFTMGTIGVVSFILPLFFKKKFANKEKIINFCAVTTWFLSTNLFPWEFLQKTPI
                     QIIQFPWRILGFQVLFGSLILIIVFLKWKTSNKKSMCSLVGIVLLIFTVTVATEANYS
                     QKIQSYKGRLIMTKKDVTLYTTSRTGGLYDYAPLDALKYKDHLKKHEVKVYD"
BASE COUNT          499 a          235 c          253 g          537 t
ORIGIN      
        1 agtctttatt cttttttgtt ttttagggat gtacaaaaga tctcaaccac aggagacatt
       61 gcttttcact tttctagaat aaaatgcttg tcctcagtat tttctggtcc aataaatttt
      121 accacattta atagctatgg cggaggcgtg aattttttta tccatatcta acatctttcc
      181 agccgtaata ttttactgga caacaaacaa tttgattcta agttatatac ttactatacg
      241 ttgggatact gaatatctgt acaattctag tgagctatca atatggacaa aagaatagat
      301 gcagccttct tattcagctg tttgtatagc ttttatggat tgatatatat tagcgtactg
      361 caatttcaga atcaattgcg ttgacgattg taccagttgt cttgtattac acatatactc
      421 ttctttatga aagaaaaaac tgcgtgattc ttttagctgt cagtatgagt ttgctcatct
      481 atacccatat tctctccacc tttatgagtg tgattgttat tggcttcttg tttatcggct
      541 ctcttttctc taagcggaaa gagagaaaag aaatgtcgaa tctgatatca aatatgataa
      601 aagctgttgg ttggaccatt cttttaacag cttacgtttg gtatccgatg ttacaacaag
      661 tcctacatca aaaaattaat cgtccttttc gcacaaaact tcaagaaagg gcattaaaca
      721 tttctgatag tttaatcggt gcagtaaata atgatttagt tacatttaca atgggaacaa
      781 ttggtgtggt ttcatttatt cttccacttt tctttaaaaa aaaatttgcc aataaagaaa
      841 agattattaa tttttgtgca gtgaccactt ggttcttatc aacgaatctt tttccatggg
      901 aatttttaca aaaaacacct attcaaatca ttcagtttcc ttggagaatc ttgggttttc
      961 aagtgctttt tggttcattg atcttaatta tagtgttttt aaagtggaaa acaagcaaca
     1021 aaaaaagtat gtgtagcctt gtaggaatag tccttcttat ttttacagtg actgtagcaa
     1081 cagaagcgaa ttattcgcag aaaattcaat cgtataaagg tcgtttaatt atgaccaaaa
     1141 aagatgttac actttataca acctcgagaa ctggaggtct ttatgactat gcaccattag
     1201 atgcattgaa atataaagat catttaaaga aacatgaagt aaaagtctat gattgatggg
     1261 agaatgcatc tcataaagca cataattcta aaataatcta tacagtagga gatgcgaatg
     1321 agcaaaatat cacgttacct gtatttaatt actgggaaca gttatgaaag taaatgggaa
     1381 aaagagcact atagaaaatg acaatggttt agtgtctatt aaaggtacgg gaaaaagaac
     1441 tatagttgag gtttcatcta cgtatccaac tacgatgatt ctatctttat taattagttc
     1501 aataactttt atattcttat ttac
//
LOCUS       DACDPS010000219         1485 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.228, whole genome shotgun sequence.
ACCESSION   DACDPS010000219 DACDPS010000000
VERSION     DACDPS010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.228"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            2..400
                     /locus_tag="IWJ07_002862"
     CDS             2..400
                     /locus_tag="IWJ07_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP9334359.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            384..1079
                     /locus_tag="IWJ07_002863"
     CDS             384..1079
                     /locus_tag="IWJ07_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAP9334360.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
BASE COUNT          558 a          214 c          302 g          411 t
ORIGIN      
        1 gatgacgatc tttgaaacgg ttgtaacaga tgttggtata gaagcagaag tattcaggaa
       61 agaaaaaata atgattttat ttggagatga agcaccggaa acattaacag attattgtta
      121 taagattgaa ttgaatcagg taagaaaaga aataacttgt gatatgaagc tctgttttga
      181 taaacaaatg tatgtaatca cagcagtagg agatgttgtc caaactaatt tgaatgaatt
      241 aggacatatc acgataaaat ttgatggatc tcatgagccg gaacttccag gaacaatgta
      301 tgtagaagat aaggaaatgc cgcatttaag aaaggggaca atcattaaaa tcgatcaaga
      361 ggatcagaat gggggaaagc tatatggaaa tcatgcttga tacagcaaat acaaaaagta
      421 tttattcttg tcaacagttt ttgaatttaa caggcgtaac aacaaatcct acaattctga
      481 aaaaagaagg agcgtcaaac ttcttctcgc atatgaaaga aatcaaaaaa ataatcggac
      541 agattcctat ccatattcag acaataggaa gaacggaaga agaaatgata gaggatgctc
      601 gagttatcat agaagaactg ggaaaagata cgtttatcaa ggtgccggtg aatcaagcag
      661 gccttcaagc gatcaaaaag ttgaaaaaag aaggttattt gattaccggt acagctattt
      721 acacagaatt acaagggtat ttagctatta ataatggagc agattatatt gcaccttatt
      781 ataacaggat gcaaaatcaa ggcattgatg ctatgaaagt gattgattcg ttgcggactg
      841 agatcgaacg tacgcaaaga ccaacaaaaa tattagcagc tagtttcaaa aattgtgaac
      901 aagtcactac agccattcga tctggagctc atgcgataac gttaggtaaa gagctactag
      961 atcaagtatt agagatgccc tcgattgata aagcagtgag gggcttccaa gaagattggg
     1021 tgcaaacttt ccaagcagaa acgattggtg aactaaaaaa ggtcgctgat tacttctgaa
     1081 tcaatttctc cataaaataa acgatagcct tctcgaaaac tcgaggaggc tatctgacga
     1141 ttcaatattc attaatttaa agtttgggtg actattactt atttctcatt taaacgatgt
     1201 attccctaac ctctaataca tccttgtaac tctgaagtta cgccagtttt gaataaattt
     1261 cataaaaaca aatgaaaagt gtcgcataaa aatatctttc gtgtttctct agtataacag
     1321 aataagagga acaagtccag aagggatatt tttagtcttt tttaaaataa cttttcagtc
     1381 gagtttgagt ttatgatagt tgtttttgaa gcgatacggc aagtgaaaaa atagggaagc
     1441 atgaaagaat ctacttaatg gcgcattgat tcatgtgata gaaat
//
LOCUS       DACDPS010000220         1458 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.229, whole genome shotgun sequence.
ACCESSION   DACDPS010000220 DACDPS010000000
VERSION     DACDPS010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.229"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            94..1038
                     /locus_tag="IWJ07_002864"
     CDS             94..1038
                     /locus_tag="IWJ07_002864"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP9334361.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
BASE COUNT          526 a          214 c          285 g          433 t
ORIGIN      
        1 caactattat aattaacatg ttgataaaat gaataaacaa gactatactt ttcatgaaag
       61 taacaaatag ttatgaaaag gagctgttta ttcatgaaaa aaacaagtcg taaagtagta
      121 atcgtcggta caggttttgt tggaacaagt atcgcttatg caatgatcaa ccaaggaatc
      181 tctaatgaat tagtattgat cgatgtgaat caagaaaaag cagaagggga agcattggac
      241 cttttagatg gtatggcttg gggcgatgaa aatgtggctg tatggtcagg tggctatgaa
      301 gaatgtaaag atgctgatat cgttgttatc acagctggta tcaaccaaaa acctggtcaa
      361 tctcgtttag atttagttaa aacaaatgcg tcaatcatgc gccaaatcgt aaaagaaatc
      421 atgggatcag gattcgacgg tattatcgtt gttgcttcaa acccagtaga tattttgaca
      481 tatatcgctt ggaacgaatc tggtctgcca acatcacgtg ttatcggtac tggaacaaca
      541 ttggatacaa ctcgtttccg taaagaaatc gcattgaaat taaaagttga cccacgcagc
      601 gtccacggct atatcttagg tgaacatggt gattcagaag ttgctgcatg gtcacataca
      661 actgtcggtg gtaaaccagt gtttgaaatt gtagaaaaag accatcgtat tgcccaagat
      721 gaattagatg tgattgctga taaagtccgt aatgctgctt atgagatcat tgatcgtaaa
      781 aaagcaactt actatggtat tggtatgagt actgcacgta tcgtaaaagc tatcttgaat
      841 aacgaacaag ctgtattgcc tgtatctgct tatttaactg gtgaatacga cgaaaaagat
      901 atctttacag gtgtgccatc aatcgttgac gaaaatggtg ttcgtgaagt tgttgaatta
      961 tcaatcaacg aagaagaaaa agcaatgttc agcaaatcaa caagtgcttt aagagaagta
     1021 ttgaacactg tattataaga aacaatgaat atgaactaaa aatcctaaaa ctgcttaaca
     1081 gctgaaatag ctgttaagca gttttttatt gatatctaat aggtaagcaa ataaacagaa
     1141 tggttaaaat taatcggtcc gaaaatgtat ccgtacatat gaacatatga aatttaatct
     1201 gttgattaca ataagcgaat cgttatcaat gtttatctgt ggttaattaa aaaatccttc
     1261 cgcaacggca aatacaatga ttcatatcta attcgttcat atttgattaa ctgaataaag
     1321 aaactggttt ttatcattca attaaacgac aaaaaaagta agaaaaactg caaaattttt
     1381 ttataatttt gcataagcag attttgatgg aaaaatgacg atttttgata aaaaaccgtt
     1441 cgagagaggt ttatcatt
//
LOCUS       DACDPS010000221         1447 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.230, whole genome shotgun sequence.
ACCESSION   DACDPS010000221 DACDPS010000000
VERSION     DACDPS010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.230"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            10..420
                     /locus_tag="IWJ07_002865"
     CDS             10..420
                     /locus_tag="IWJ07_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP9334362.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            434..790
                     /locus_tag="IWJ07_002866"
     CDS             434..790
                     /locus_tag="IWJ07_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAP9334363.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          533 a          218 c          268 g          428 t
ORIGIN      
        1 agtgagaaaa tgataaaagt atatacttcg cctagctgta cttcctgtag aaaagcaaga
       61 gcatggttgc aagcgaatca acttgaattt gaagagaaga atatttttgc cgaaccgtta
      121 acagaaagtg agatcaaaaa gatcttggca gcgactgaag gaggagttca agatattatt
      181 tccactcggt cgaaaattta cgaaaagctg ggcatagatt ttaacgaatt aactttaaag
      241 caaatgatcg tcttattcaa agaatatcct tcattattga gacgacctat tttgattgat
      301 gaaaaaagaa tactaatcgg atttaatgaa gatgagatcc ggtcatttgt ccctcgtgaa
      361 attcgatgtg ttgccagaaa tctgatctat ttacaaaaca ataacatctt aaattcatag
      421 ggagggagcc ttcgtggaag tgaaagtcta tggatcagcg cactgtgtaa caagtctgaa
      481 ggcgaaacaa tggttagaac accatcaatt gccttatcaa tttgttgact tagaagaacg
      541 cgatctaagt acagaagaag cagaagaatt atgcagtttt gaagaagtgc aagcagaaca
      601 gctatttgcg acttggtctg aggcttttca caacattgcg attgaccttt cctgtgtagg
      661 aaaaaaacaa ctgatttttt tatgcacaca acataccaat ctcctcagac gtcctttgat
      721 tattatcgat gaagttctat ttattggata caatgagcag ttgatggaac agcttatctt
      781 aaataaatag actttaactg ttttaatcaa gtcactgagc atttcataca gttgtaacag
      841 tcgaacactt agcatcaagc gtgttatgtg ttcttttttt gactttattt ttctactata
      901 gagacatgct caatttatgt ggtcaaaatt caaatctagg tggggatata gagttaaaaa
      961 tatattgaca tgtgtaaatt tacatgatat attcatttct gttgttttaa gtagcaaagc
     1021 aataaaatag tattttaaaa aacatataaa atagttgttg acatgttaga aatgacatga
     1081 taagataact gagttgttaa aaaagaacca cttgtgaagc ttgattctat cgaaaaaaac
     1141 ttcaaaaaaa gttcttgaca aataacaaat gataagttat gataaatgag ttgctgtaag
     1201 atttcgaaaa acgaaatcaa aaaaactttt aaaaaagagt tgacaattac ttgtctctct
     1261 gatatgatat aaaagttgct acggcagcca aaaacgaatc ggggaacact cctgatgaag
     1321 tagacctttg aaaactgaac aaagtaagac aaaccaaatg tgtagggcgt cttgattcaa
     1381 ttcaagacaa caaacatttt ttaacaagca agcaatatgc tagcaaacaa attgagctta
     1441 acaatcg
//
LOCUS       DACDPS010000222         1446 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.231, whole genome shotgun sequence.
ACCESSION   DACDPS010000222 DACDPS010000000
VERSION     DACDPS010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.231"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(137..427)
                     /locus_tag="IWJ07_002867"
     CDS             complement(137..427)
                     /locus_tag="IWJ07_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334364.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(545..805)
                     /locus_tag="IWJ07_002868"
     CDS             complement(545..805)
                     /locus_tag="IWJ07_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334365.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(942..1355)
                     /locus_tag="IWJ07_002869"
     CDS             complement(942..1355)
                     /locus_tag="IWJ07_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAP9334366.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
BASE COUNT          531 a          238 c          214 g          463 t
ORIGIN      
        1 ttgttactat gaataacata ataagaaagt aaagtggatt gatttgagat ctttttatac
       61 ttcaaaaaag ataacaccca actttcaaaa atatgttttg gaagttggga caaacgagtt
      121 aatacatttt aatacattat tttttattca aaaaaaatag cacaatggca aagatcgtaa
      181 gagagatcat aagccccaat gaatgactaa aaagaaaagc agaatcacat gccatatcat
      241 cccatatttc ttcaagaaaa taaataagcg ggaaaaaggg aaggaatagt agaacatcga
      301 tccagcacca tttttttctt cttttgaaat aacataaaac aataaacatg aacgtgcagg
      361 gaagtaaaaa caaagcttta ctcataacta acaaaatacc agctcctttt atttttttct
      421 cccccacatt caaagagtaa caatggtatg tacttaataa tagaaaaatg gatcaacttg
      481 tatgaataac agctgaaaag atctgcacca aataaaaaac ttccagccaa aactgactgg
      541 aagtttattt ttttctgaaa tattctaaca caaatatgaa aaagatggcc gaagctaata
      601 agccgatttc gtagcaaaac aatgagatac cagagtcgta ataataaaat cctctaatct
      661 tatggataaa atagatcatc ggacaagcag ataagagtaa gtatttgtta ggccttttga
      721 ataagtaaaa gaaagcactg gtcattatga taaatggtat ccaaaacaaa agtttactta
      781 agacaatcaa atgatcaact cccatatttt ttaccaagag agatcataac atctttatcg
      841 taaaaacaaa atattttttt gctcattttg aaaatttaaa catactcgtt cttatccaaa
      901 tatataaaaa tcgcccctca ttcatcgaga ggcgataagt tctaatttaa ctcaattttt
      961 tttgtttctt ttggctgata agctgtttta ggtaatacca atttcagcac accgttttta
     1021 aaggttccag tgattttttc ttcatcaaca ttaggaagat agaatgaacg tttgtaagag
     1081 accgaatgtc tttctttccg tacatagttg ccatcttctt tatcttctgt atgggactca
     1141 tgattggcgg agatcgtcaa agtattatct gaatattcaa ctgtcgtgtc ttctttgtca
     1201 cagccaggca tatcagcagt tagtttgtat tctttttcat tttcgactaa atctactttt
     1261 ggataacttg aaacacctaa aaaatcattg aatgctggac taaacacgtc attgaaatca
     1321 gggaacatgt cgcgaatgct aggtacattt gccatttttg atcatccttt caataagtat
     1381 tttttattta tccctattta cgtaataact ctattttgaa aaaaatgcaa gtataatggt
     1441 tagctt
//
LOCUS       DACDPS010000223         1428 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.232, whole genome shotgun sequence.
ACCESSION   DACDPS010000223 DACDPS010000000
VERSION     DACDPS010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.232"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..242
                     /locus_tag="IWJ07_002870"
     CDS             <1..242
                     /locus_tag="IWJ07_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP9334367.1"
                     /translation="NGTYDFDRFYQGKGDINAQILFSYKAEQYYKGYKKHEHEFVPEV
                     KKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            253..>1428
                     /locus_tag="IWJ07_002871"
     CDS             253..>1428
                     /locus_tag="IWJ07_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9334368.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFNVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSLLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKSQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     "
BASE COUNT          504 a          274 c          301 g          349 t
ORIGIN      
        1 cgaatggtac gtacgatttt gatcgttttt atcaaggaaa aggggatatt aatgctcaga
       61 ttcttttctc ttataaagca gaacaatact acaaaggcta taaaaagcat gaacatgaat
      121 ttgtacctga agtgaaaaag gattggaacg atcaattgaa gcatgagatc cagcaacaag
      181 aaattagaaa gcaaaaacgt gcgatgttgt ttcaacaagg cagacaacaa gaaagggagt
      241 gagatcgtca cgatgaaaac tgtcatttta gcagaaaaac cgtcacaagc gaaggcttat
      301 gccgatagct tttctaaagc cacccgaaaa gacgggtatt ttgagatcca agatcgattg
      361 tttcctggtg aaactgtgat tacgtatggt tttggtcatt tagtcgagtt agattcgccc
      421 gatatgtacg atgaaaattg gaaacaatgg tcattagaac atttacctat tttccctaca
      481 cattatcact atcatgttcc taaagataag aaaaagcaat tcaacgtggt gaaacagcaa
      541 cttcaatcgg ccgacaccat tattattgcg acagatagcg atcgcgaagg ggaactgatc
      601 gcttggacga tcattcaaca agcaggcgcc gatcatggaa agatatttaa aagactttgg
      661 atcaactcct tagaaaaaga agccatttat cagggattcc agcagttacg agatgcagaa
      721 gagacctacc caaaatttga agaagcacaa gcacgccaga tcgccgattg gttgatcggc
      781 atgaacggta gcctactgta tagtttactc ttgcagcaaa aaggcatacc aggaagtttt
      841 tccttaggca gagtccaaac cccaacgctt tacatgattt atcaattaca ggaaaagatt
      901 agaaacttcc aaaaagaacc atattttgaa ggaaaatccc aagtaatcgc tcaaaatggc
      961 gcatttgatg cgaagcttga tccgaatgaa acccaagcga cgcaagaagc atttgaagat
     1021 tatttaaaag aaaagggggt tcaattggga aaacagccag gtacgatcca ccaagttgaa
     1081 accgagaaaa aaagtgcggc aagtccacgt cttttttctc tatccagcct tcaatctaaa
     1141 atgaatcagc tcatgaaagc tagtgccaaa gatactttgg aagccatgca agggttgtat
     1201 gaaggaaaat atctaagcta tccgcgaacg gatacccctt atattacgga aggggaatat
     1261 gcgtatctgt tggatcactt agacgaatac aagcattttt taaaagccga agcgattccg
     1321 actcctattc atacaccgaa tagtcggtat gtgaataata agaaagtcca ggaacattac
     1381 gcgatcattc ctacaaaaac ggttatgaca gcagcggctt ttgaacaa
//
LOCUS       DACDPS010000224         1428 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.233, whole genome shotgun sequence.
ACCESSION   DACDPS010000224 DACDPS010000000
VERSION     DACDPS010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.233"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..242
                     /locus_tag="IWJ07_002872"
     CDS             <1..242
                     /locus_tag="IWJ07_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP9334369.1"
                     /translation="DGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPEV
                     KKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
     gene            253..>1428
                     /locus_tag="IWJ07_002873"
     CDS             253..>1428
                     /locus_tag="IWJ07_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9334370.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     "
BASE COUNT          505 a          272 c          300 g          351 t
ORIGIN      
        1 tggatggtac gtacgatttt gatcgttttt atcaagaaaa aggggatatt aatgctcaga
       61 ttcttttctc ttataaagca gaacaatact acaaaggcta taaaaatcat gaacatgaat
      121 ttgtacctga agtaaaaaag gattggaatg atcaattgaa gcatgaaatc taccaacaag
      181 agatcagaaa gcaaaaacgt gccatgttgt ttcaacaagg tagacagcaa gaaagggagt
      241 gaaatcgtca tgatgaaaac cgttatttta gcagaaaaac cgtcacaagc gaaggcttat
      301 gccgatagct tttctaaagc tacccgaaaa gatgggtatt ttgagatcca agatcgattg
      361 ttttcaggag aaactgtgat cacgtatggt tttggtcatt tagtcgagtt agattcgccc
      421 gatatgtacg atgaaaattg gaaacaatgg tcattagagc atttgcctat tttccctaca
      481 cattatcact atcatgttcc taaagataag aaaaagcaat tcaaggtggt gaaacagcaa
      541 cttcaatcgg ccgacaccat tattattgcg acagatagcg atcgtgaagg ggaactgata
      601 gcttggacga tcattcaaca agcaggcgcc gatcatggaa agatatttaa aagactttgg
      661 atcaactcct tagaaaaaga agccatttat cagggattcc agcagttacg agatgcagaa
      721 gagacctacc caaaatttga agaagcacaa gcacgccaga tcgccgattg gttgatcggc
      781 atgaacggta gcccgctgta tagtttactc ttgcagcaaa aaggcatacc aggaagtttt
      841 tccttaggca gagtccaaac cccaacgctt tacatgattt atcaattaca ggaaaagatt
      901 agaaacttcc aaaaagaacc atattttgaa ggaaaagccc aagtaatcgc tcaaaatggc
      961 gcatttgatg cgaagcttga tccgaatgaa acccaagcga cgcaagaagc atttgaagat
     1021 tatttaaaag aaaagggggt tcaattggga aaacagccag gtacgatcca ccaagttgaa
     1081 accgagaaaa aaagtgcggc aagtccacgt cttttttctc tatccagcct tcaatctaaa
     1141 atgaatcagc tcatgaaagc tagtgccaaa gatactttgg aagccatgca agggttgtat
     1201 gaagggaaat atctaagcta cccacgaaca gatacccctt atattacaga aggggagtat
     1261 gcgtatctgt tggatcactt agacgaatac aaacactttt taaaggccga agccattcct
     1321 actcctattc atacaccgaa tagtcgatat gtgaataata agaaagtcca ggaacattac
     1381 gcgatcattc ctacaaagac ggtcatgacg gcagcggctt ttgaacaa
//
LOCUS       DACDPS010000225         1373 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.234, whole genome shotgun sequence.
ACCESSION   DACDPS010000225 DACDPS010000000
VERSION     DACDPS010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.234"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            191..445
                     /locus_tag="IWJ07_002874"
     CDS             191..445
                     /locus_tag="IWJ07_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP9334371.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="IWJ07_002875"
     CDS             446..763
                     /locus_tag="IWJ07_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP9334372.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          493 a          196 c          266 g          418 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaagacaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accttaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaagtcc aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat
     1201 acttccctgc tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa
     1261 tgggataaaa tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat
     1321 agtgaacata cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat ata
//
LOCUS       DACDPS010000226         1364 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.235, whole genome shotgun sequence.
ACCESSION   DACDPS010000226 DACDPS010000000
VERSION     DACDPS010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.235"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            82..>1364
                     /locus_tag="IWJ07_002876"
     CDS             82..>1364
                     /locus_tag="IWJ07_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAP9334373.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          465 a          262 c          257 g          380 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taat
//
LOCUS       DACDPS010000227         1343 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.236, whole genome shotgun sequence.
ACCESSION   DACDPS010000227 DACDPS010000000
VERSION     DACDPS010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.236"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..370
                     /locus_tag="IWJ07_002877"
     CDS             <1..370
                     /locus_tag="IWJ07_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334374.1"
                     /translation="DLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENI
                     DLENEISKLESIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFL
                     TKLVSEWKSIISQNMDKLRY"
     gene            641..1264
                     /locus_tag="IWJ07_002878"
     CDS             641..1264
                     /locus_tag="IWJ07_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9334375.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          524 a          168 c          236 g          415 t
ORIGIN      
        1 tgatttaaaa aatgaccata aagaaagaag aaaaattgaa gaatatactt ctttaatgga
       61 aaagtgtaaa gcaactgatg caatttttag ctttttaaga aaaagtgatg agataatcac
      121 acgtgaaaat atagatttag aaaatgagat tagtaaactt gaatctatct tttccgatat
      181 aaattctctt aagtatttaa tcgtcgaaga aaattcggat agctttttag aatttgaaaa
      241 atatctaaca gatcttatag ataatagaaa taatgaatta atgagacaaa acattgcaag
      301 attccttact aaattagttt ctgaatggaa aagtattatt tctcaaaata tggataaatt
      361 aagatattaa ggatgttagt tttaaaataa actaatgtcc ttatttttgt gttcacattt
      421 ctgttaccta agaaatactt aaaggaggtg atgtagccag caacgactac aacttgaacc
      481 cgctatctac agtttatttg ttgtttcttt tgggtatact ctaatggtac aaatccatat
      541 ttgcttaaag cgttgatttt tcagcggttt ttctttattg caaagtgtac cctaagacct
      601 gtatactaat agtaaataga ttggatagga atgatggcaa atgtatttag cttatttaag
      661 agtttcttcc gacgatcaaa gtttagctcg gcaacataag ctaattgaaa attggtcagt
      721 caaaaataat attgctgaag acgatttaaa aattttcgag gaaaaagtct ctggaaaaaa
      781 tattgaaaac agaccagagc tgcaagagct tatttctttc atacgtgaac gagatacgat
      841 tattattcct agtcttgatc gattagcaag aaatagtaaa gatacaaaag acttacttca
      901 attgtttcgt agtaaaggtg cttctattga aatattagac ctaccgtcgt ttaatggggt
      961 aactgaccct gcattaagag acttgctaac aaatttagtc atagaagtgt ttagctatgt
     1021 tgctgaaaac gaacggaaaa aaatcaaaga gcgtcaaaaa caaggcattg aaattgcaaa
     1081 gaaaaatggt cgttatactg gtaaaaagat tcaataccat gccaatgcaa caggaaaaaa
     1141 caaattgatt tacgatgaaa ttgttgatgg tctgaagaaa aaagaaacta tttctaaaat
     1201 agcaaagaac gcaggagtta caagaaaaac cgtttatcga gtaaaaagag atttaggaat
     1261 gtaatatgtt ccagttagaa agaagggttc tgttgcaaag ttttaaatct actatcaaat
     1321 aaggtagaat aatagaaaaa gat
//
LOCUS       DACDPS010000228         1337 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.237, whole genome shotgun sequence.
ACCESSION   DACDPS010000228 DACDPS010000000
VERSION     DACDPS010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.237"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            109..927
                     /locus_tag="IWJ07_002879"
     CDS             109..927
                     /locus_tag="IWJ07_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP9334376.1"
                     /translation="MDTFQGTTFTVGNFKGGVGKTKIVSMLAYDNAMIRNRKTLIIDL
                     DPQANLTQVIARTFGITDIKTTITNGVANGELASSIIKVNENLDLLACDTSFRSFSAF
                     ANKHENELDQVTVLKKLLLPIKNNYQEIFIDVPPTISEFSDNAMAASDYSIISFQTAE
                     ESLEGVNKYVNYQKFMVDRYDLDLQIIDIVPCMVEPNDDFDEEILEEAIEKYGTVVSK
                     NLIHYQKRLRRYSKIGIHLRKYKNGNFDQWDYKAHQVFINILAELDARQEFLNK"
     gene            946..>1337
                     /locus_tag="IWJ07_002880"
     CDS             946..>1337
                     /locus_tag="IWJ07_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334377.1"
                     /translation="MPKDFDIPKMMENGEFEPLLPNNKRQDVKISGEYNFSNSALSNT
                     RNITLKKRGRPVSIPDKRNKVSVPKKISPALNSKLSILQDYMTELQSEKGRISFEKIV
                     NTLADSYIQHRLGVAKEEHIREEIQEEF"
BASE COUNT          532 a          189 c          226 g          390 t
ORIGIN      
        1 atatccaaac gtttttagat attattattt aagttatact tttaatgttg accatttccg
       61 atatataagt tatactttaa ttatcaatta aagattggga ggatttacat ggatactttt
      121 caagggacta catttacagt tggcaatttt aaaggaggag taggtaaaac taagatagtc
      181 agcatgcttg cttatgacaa tgcaatgata agaaatagaa aaacgcttat tattgattta
      241 gaccctcaag cgaacttaac acaagtcata gcaagaactt tcggtataac cgatatcaaa
      301 accactataa caaatggtgt cgcaaatgga gaattagcaa gtagtattat taaggtaaat
      361 gaaaatcttg accttctggc atgtgacact agttttcgat cattctccgc ttttgctaat
      421 aaacatgaaa atgaattaga ccaagttact gtcttgaaaa aattacttct tccaataaag
      481 aacaattatc aagaaatatt tattgatgta ccaccaacca ttagcgaatt ttccgataat
      541 gctatggcag cttcagacta ttcgataatt tcatttcaaa cagctgaaga atccttagag
      601 ggagtcaaca aatatgtaaa ttatcaaaaa ttcatggtcg atagatacga tcttgatttg
      661 caaattatag atattgtacc ttgtatggta gaaccaaatg atgattttga tgaagaaatc
      721 ctagaagaag caattgaaaa atacggaacc gtggtatcca aaaatctcat tcattatcaa
      781 aaaagattga gaaggtattc taaaattggt atacatttaa gaaaatacaa aaatggaaat
      841 tttgatcaat gggattataa agcacaccaa gtatttataa atattttggc agaactagat
      901 gctagacaag agtttttaaa taaataataa ggagttgcat aagtaatgcc taaagatttt
      961 gatattccta aaatgatgga gaatggtgaa tttgagccat tattacccaa taacaaacgc
     1021 caagatgtga aaataagcgg tgaatataac tttagcaata gtgcattgag taacacaaga
     1081 aatattacat tgaaaaaaag aggccgacca gtttcaattc ctgataaaag aaacaaggta
     1141 tcagttccta aaaagatctc tccggcatta aatagcaaac tctcaatttt acaagactat
     1201 atgacggagt tacaaagtga aaaagggaga atttctttcg aaaaaattgt taatacatta
     1261 gcagactcat atatccagca tagactagga gtagcaaaag aagaacacat aagagaagaa
     1321 atacaagaag agtttga
//
LOCUS       DACDPS010000229         1324 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.238, whole genome shotgun sequence.
ACCESSION   DACDPS010000229 DACDPS010000000
VERSION     DACDPS010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.238"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            278..1324
                     /locus_tag="IWJ07_002881"
                     /pseudo
     CDS             278..1324
                     /locus_tag="IWJ07_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180785.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
BASE COUNT          533 a          157 c          247 g          387 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcaaat tgatgaagca gatcccaaag ggctgcttca tcaatttttt
      121 cgtaagtgtc cgtacactta ctgtgcttgc tcatatatta atcgatcata tactaatcgt
      181 gtaagaacaa ctttaagcat aatacttata ttgttatacc atagtttgga aaaataaaaa
      241 attatatttc atcaacgttt cataaaaggt gtgtagaatg ttattgaaag tagctataaa
      301 atgctattgt gtaaaaaata aaggaggaat aatttatgaa aaaatagtac caagaggagc
      361 atgcctatta cttgcattat gtttgagctt aagcgcagtt gggtgtcaaa aaagtaatga
      421 aaacaataaa ataaaagaag gacaatccat aagttctgaa gaggaaggca tgtcaattaa
      481 tgcaaatggc aaaaatgaaa cttttaagcc atcagattat acattggagg caaagaaaga
      541 atatgtttat gaatatttag ggcttaaatt taaactctca gacaaattta gaaattatat
      601 agctgataaa aagatagcga tgttagatga tcaaagtcct atagataaag aactaaagta
      661 tgctattttg acatttgaga aaatgacaga agaacaaaag aatgcagtta tagaaaagat
      721 gggcgatgga tataaaaatt ggcaaaatga gcttgaaaga attgggacta ttggtatatt
      781 tgaaaaaaat acatcagaag aaaaaaatct aaaattataa aatgtgatac tcatactaaa
      841 ataggagttt caagtgatgg aaaatacgat tgctatttaa gcacaaatag cggagctgaa
      901 agtaatcttc ttgatgaatt aaaaagaact gagattcaaa ttatagataa aaaagaacgt
      961 cctaaaaatg gttttgtgct atcagaaaaa actgatttag aaaatacaga agcatttaac
     1021 aaagaatcgg ttaaagattt acgtaaacta ccaacaaagg acataaatgg caaggatttt
     1081 acaagtaaag attttgagaa gtatgatctt actatggtaa atgtatttgc aacttggtgt
     1141 acagcttgtg taaaggaaat tcctgatttg gttgaggttc agaatgaaat gaaaagcaag
     1201 ggtgtaaata tcgttggagt tgttacagat cctgttgatg ataacggtga aaacaaggaa
     1261 gcaattgaaa agtcaaagtt aatacatgaa aagacaaagg cttcatatcc tttcttaatg
     1321 ccag
//
LOCUS       DACDPS010000230         1316 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.239, whole genome shotgun sequence.
ACCESSION   DACDPS010000230 DACDPS010000000
VERSION     DACDPS010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.239"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            223..1011
                     /locus_tag="IWJ07_002882"
     CDS             223..1011
                     /locus_tag="IWJ07_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP9334378.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTTAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            998..>1316
                     /locus_tag="IWJ07_002883"
     CDS             998..>1316
                     /locus_tag="IWJ07_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334379.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNYKPKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQN"
BASE COUNT          533 a          189 c          226 g          368 t
ORIGIN      
        1 tcgcaaaaca tatttcttat gattaataaa aaattttaaa gtaataaaaa gtgattattt
       61 tactttcgaa taacatatga ataatcttta tatccgaacc gatatatatc tttaaaacaa
      121 aataatcaca caatattaca atataatgca tatacgatat tgcaatatca caatattatg
      181 atataattat attataatat aagatgaatg ggggatattt taatggcaag aatattgtca
      241 gtagccaatc aaaaaggtgg agtttctaaa actacgacta gcgttaattt agcagcatgt
      301 ttgaaatttg tttacggtaa aaatgtactt ttagtggaca ttgatcctca gggaaatgct
      361 acagataatg taggtttcga tatagatgga acaaaccaac ctacaattta tgaagtatta
      421 aaaggggagg ctgacattac agatgctatt ctggactata aaggaattga tgttttgcca
      481 gccgacatag ccctttcttc tgcagagagg gaatttactc aagtaggaag tgaacacagg
      541 cttaaaagag tacttcaacc aatcaaggac aactatgatt atattataat tgattgtcca
      601 ccaagtttag gaatgcttac agtcaacgca tttactgttt ctgatgaaat tatcattcca
      661 gttgaagctg cgtatttctc attaaaagga ctaataaagt taagtgaaac tattgaaacg
      721 gtaaaagaat atacgaacga caagcttgaa gtacgaggag tcctatttac taagtataat
      781 aagcgttata acatttctaa agaaatgacg actgcagcaa ccgaaatttc tagagtcatt
      841 ggtgcaaaaa ttttcaaaac ttatattcgt agaacaataa ccgttgacga agcgcaagct
      901 gctggatcag atctaatcaa ttttaataaa ccctcaacag ctgaagatga ctataaagca
      961 tttacagaag aatatttaaa agaagttggt gattttgatg gcagaagata atcgcagtca
     1021 agctgcagta agtagactac taggaaataa aaataaaaag aactacaaac caaaagaagt
     1081 agaagaaatc gaattaacca agaaaaatat aagtaaagtc ataaaagatc aaggaagtag
     1141 tgtaagaaca gcagatagca ttcgtataaa tccaattcta atgtcagcac tcaaatattg
     1201 gacgactatt gctgaatcag acaaaagtaa accagacgtt atagaagatg cgctattaaa
     1261 atccatacct gaagaatact tgattgaagg atataaacta gcaaagaaac aaaata
//
LOCUS       DACDPS010000231         1291 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.240, whole genome shotgun sequence.
ACCESSION   DACDPS010000231 DACDPS010000000
VERSION     DACDPS010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.240"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(167..>1291)
                     /locus_tag="IWJ07_002884"
     CDS             complement(167..>1291)
                     /locus_tag="IWJ07_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAP9334380.1"
                     /translation="SIIKEYRSDRTTYFFYDYKKLLTDNIKKTKDFHFFTGDFNEMFL
                     LYMFKTRYYLFGPFRANNIDKVFFKLKMNNLNVAMADRERLYNSLQNLTLYSLGDIRD
                     ILILVHYFFTGKIEDLFHEPLIEYTENLSKTIEQIKIDNLLSQNYDPEIYLFLYENKI
                     LEYVKNGDIRNLENMVFNLSNGIIPSVSGDTIRSEKNYSIIVFEKLAQTSITLGMDII
                     EAYQSRDALIQENELAVSLPEVLKVRDSGIVYYTKEIGKTKIEHLSPLISSVVQFIGL
                     NIYKRITVKEIANYFSVSETKLRESFKNEMHITIYNYISKRKISTAKIMLKSNHTISE
                     VSLGLGFSDSSHFSRVFKKYAGVSPKQYQLGLVDNFNNIS"
BASE COUNT          456 a          212 c          148 g          475 t
ORIGIN      
        1 tttgtcatat tcatttaaca cactattatt accattactt agacgctcag attctacgaa
       61 acaactcaaa tcagaaattt cccaaaaaaa ttttttgtta aaatccaatt tcctcaactc
      121 ctccaaacta cgtaaatgat atagtctcat aataaaataa aacctctcag ctaatgttgt
      181 tgaaattgtc cactaaacct aattgatatt gcttaggaga tacacctgcg tactttttga
      241 atactctcga aaagtgtgaa gaatctgaaa atcctaaacc taaagaaact tcactgatag
      301 tatggtttga tttcaacatt attttagccg tagaaatttt tcgcttagag atataattat
      361 aaatcgtaat atgcatttca ttcttaaagc tttctcttaa tttagtttca cttacagaaa
      421 aataattagc tatttccttt acagtaattc ttttatagat atttaagccg atgaattgaa
      481 cgacagaaga aattaaggga gaaagatgct ctatcttagt ttttccgatt tccttagtat
      541 aataaacaat cccagaatcc ctcactttta agacctcagg taacgataca gctaattcgt
      601 tttcttgtat caaagcatct ctactttgat acgcttctat tatatccatt cctaatgtta
      661 tagacgtttg tgctaacttt tcaaaaacaa tgatagaata atttttttca gaccgaatcg
      721 tatctccact aacacttgga attattccat tactcagatt aaaaaccatg ttttctaaat
      781 ttcgaatatc tccattttta acatattcca gtattttatt ttcataaaga aacagatata
      841 tttctggatc gtaattttgt gataacagat tatctatttt gatttgctcg atagttttac
      901 ttagattttc tgtatattca attaacggtt catggaatag atcttctatt tttccagtaa
      961 aaaaataatg aactaaaatc agaatatctc gaatgtctcc taaagaatac aaagtcaaat
     1021 tttgcaatga attatagagt cgttctctat cagccattgc aacattcaaa ttgttcattt
     1081 ttagtttaaa aaaaacttta tcaatattat ttgctctaaa tggaccaaat aagtaatatc
     1141 tggttttaaa catgtataga agaaacatct cattaaaatc accagtaaag aaatggaaat
     1201 ctttagtttt tttaatatta tcagttagta actttttata atcataaaaa aaataagtag
     1261 ttctatctga gcgatactct tttataatac t
//
LOCUS       DACDPS010000232         1269 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.242, whole genome shotgun sequence.
ACCESSION   DACDPS010000232 DACDPS010000000
VERSION     DACDPS010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.242"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            187..753
                     /gene="amaP"
                     /locus_tag="IWJ07_002885"
     CDS             187..753
                     /gene="amaP"
                     /locus_tag="IWJ07_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP9334381.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            766..954
                     /locus_tag="IWJ07_002886"
     CDS             766..954
                     /locus_tag="IWJ07_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP9334382.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            967..>1269
                     /locus_tag="IWJ07_002887"
     CDS             967..>1269
                     /locus_tag="IWJ07_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9334383.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIY"
BASE COUNT          447 a          194 c          270 g          358 t
ORIGIN      
        1 aaaagtaaat agagaataag taagcaattg ttatttcagt atcagactgg caataaaacg
       61 ccgttatgat ccagcaatgt ttggtagcgc tttatttact gatgctatgc taactgttcg
      121 ctatctatac tgtgattgtc ggtagagtta tttacagggt aaaaataagt ttataggagg
      181 tggaaaatga ataaggggtt aaaaataatc ggtgttatca tgagtttact gctactttca
      241 gtatttctag cgattgcgtt gatcaatgcg ccgtatatta tgcctgaaca attggaacga
      301 tttcgcttct ttactttaac gaattactac atgcaacaat atattttctg ggcagctgta
      361 gtatttgccg ttcttgctat tatcatcttg ctagtcgttc ttttctatcc aaagtcaaga
      421 ggaactttcg ttatgaaacg ggaagatggc aaactaacaa tcgacaaaaa agcgatcgaa
      481 gggttggtac gttcccattt acacgaagaa gagttcattc attcacctaa agtacggata
      541 cgttcaacga aaaatcgaat ccatatccat gtcaacggag acttgaaacg aacttcttca
      601 ttagtaggga aaacaggtgt attgatgcaa gatatcgaag aagaagtaac taaagtactc
      661 ggaacaaaag aaacggtcaa agtggcagtc acatacagcg gttatgaaga acaagaagat
      721 acaagagact acaagcactc tcgagtggaa taggagggcc aaataatgaa agaactattt
      781 tcagcataca agctccctat tatctttggg ggtcttggac tactgctagc tgttttatgg
      841 ttaacgattg gatttttcaa gacattgttg atcataatca tgacagcaat tggcgcagga
      901 atcggttttt atttgaaaca gtcaggaata gttgatcaat tttttaaatc ataggaggaa
      961 acaaagatgg aaaacaaaaa catgaataca acaaatggat cagttttggc aaatgaaaaa
     1021 ggagaaaacc gcatgatgaa tggtgaattg acttatgaag acaaagtcat ccaaaaaatc
     1081 atcggttatg cattgaaaga tgtagatggt ctattgacat tagatggcgg atttttctca
     1141 aatatggctg aaaaattagt caacacagat aacgtaacag caggtatcaa tacagaagta
     1201 ggtaagaaac aagtagctgt ggatctagat gtgatcgtag aatacggtaa agacatcgaa
     1261 gatatttac
//
LOCUS       DACDPS010000233         1244 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.243, whole genome shotgun sequence.
ACCESSION   DACDPS010000233 DACDPS010000000
VERSION     DACDPS010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.243"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            129..683
                     /locus_tag="IWJ07_002888"
     CDS             129..683
                     /locus_tag="IWJ07_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9334384.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
     gene            937..>1244
                     /locus_tag="IWJ07_002889"
     CDS             937..>1244
                     /locus_tag="IWJ07_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334385.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     "
BASE COUNT          508 a          129 c          220 g          387 t
ORIGIN      
        1 gatataaaat cataaactaa accccttgat tttcttcact tttaacagag tatatatata
       61 agttcatgat tatggtaaaa tatatatata atcatgaact cagaatattt ttaagaaggg
      121 aaacaatagt gagctataat gtacaaccgt taaggacaaa tgaagagata caagattttc
      181 agttttggct aagaagaaca agtaatccag aaagagatag ttttttattt ttgtttggaa
      241 tcaataatgg gttacgtatg tcagatatta taggattaaa agttggagac atacgaggta
      301 aaagtaagcc tattattgtt gaacataaaa caggtaagcg aaaaccgatt tttatagata
      361 atttgagaga agagatttta ttatatactg aaggtaaaga agaaaatgat tggttatttc
      421 caagtcggca acaaggtcga catataacaa gggatcgtgt ctatcagata tatgcagata
      481 tagcagaaaa attaggaaga gatgatatag gaacacatac attaagaaaa acttttgggt
      541 atcactacta caagaaaact agagacattg ctaccttaat gtttattttt aatcatagct
      601 cgcaagcaat tacaaaaagg tacattggta taactgaaga tgaaataggg gcatcgttac
      661 gtggttttaa attgggaggc taattgagat attaggatat attttttgtg tataatgaaa
      721 acttagaagg tgagattaat gaagataact gaattttcgg aagtatttaa aacgtttagt
      781 aacatttaca atagaatacc agagatagaa caaaatgttg tagttgataa tgattattat
      841 atagagaatg attttaataa accaagaaaa tatgacaacc tatacaattt tatacacaat
      901 agaacaacaa taaataacaa cagtaacact cgagttttga ttaattcagc atattttcat
      961 attaaatatt taaagaaata tgagtatgaa gaagttgtat tagattatgg gctaaataga
     1021 cagtcatttt taatttatgc aataaataac ggaaatttgc caagcaataa aaagaacata
     1081 attattgaaa catatgccta tctggagggg ggatctaaaa aactaattaa gtcatgtaat
     1141 atagagtatc aattatcagt aggagaaatt aaatgtgttt tcgaagaaga tttagaactt
     1201 gacttaaaaa aaatttttgc cagcattcat gattatatag gttt
//
LOCUS       DACDPS010000234         1215 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.245, whole genome shotgun sequence.
ACCESSION   DACDPS010000234 DACDPS010000000
VERSION     DACDPS010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.245"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..>1215)
                     /locus_tag="IWJ07_002890"
     CDS             complement(<1..>1215)
                     /locus_tag="IWJ07_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HAP9334386.1"
                     /translation="LEEKIGQLFVNMGSSRDKNYLSSMVNQYHIGAVRYNPGTADEVY
                     DQNYILQTESKIPLLIAANTEAGGNGACTDGTEVGVEVKIGATNDAKYAYELGRVSGI
                     EASAIGCNWSFAPIVDINRNWRNPIISSRSFGQSPDKVLELSLAYMKGIQESGILPAA
                     KHFPGDGIDERDQHLSFSINSLSTEEWDNSFGKVYSGLIEAGLPSIMAGHIHMPAYSK
                     HFNLDLKDEDLLPATLSKELITDLLRGKLGFNGLVVTDASHMLGMTGAMKRSELLPTS
                     IAAGCDLFLFFNDPDEDFNYMMEGYYKGIISEERLRDALRRILGLKAKLGLHKKEKNK
                     ILLPKQEAMEKIGLIENKSIFKEVADQAITLVKDKQDIWPIVPEKYKRILLVDVHGVK
                     GGFGEVVAGNRP"
BASE COUNT          380 a          254 c          154 g          427 t
ORIGIN      
        1 tgaggtctat ttccagcaac tacttcacca aacccacctt ttaccccatg tacatctacc
       61 agtaaaatcc ttttatattt ttctggtaca ataggccaaa tatcttgttt atctttaact
      121 agtgtaattg cttgatcagc tacctcttta aaaattgatt tattttcaat taaaccaatt
      181 ttttccatag cttcttgttt cggtaacaaa attttattct tttctttttt atgcaaacct
      241 agtttagcct ttaagcctaa aattcttctt aatgcatcac gtaaacgctc ttctgaaata
      301 attcctttgt aataaccttc catcatataa ttaaagtctt catctggatc attaaaaaat
      361 aggaatagat cacatccagc cgcaatagaa gtgggcaata attctgaccg tttcattgcc
      421 ccagtcattc ctaacatatg actagcatca gtaactacta atccattaaa cccgagtttt
      481 cctctaagta agtcagtgat taattctttt gataaagttg caggaagtaa atcttcatct
      541 ttcagatcaa gattaaaatg ttttgaataa gctggcatat ggatatgacc tgccataatt
      601 gaaggtaatc cagcttctat taagcctgag tatacttttc caaaagaatt atcccattct
      661 tctgtactta aactattaat actaaaagat agatgttgat ctcgttcgtc aataccgtca
      721 ccagggaaat gtttagctgc aggcaaaatt ccactctctt gaataccttt catataagct
      781 aaactaagtt ctaaaacttt atcagggctt tgtccaaaag atcgactaga aatgatagga
      841 tttctccaat ttctattaat atctacaata ggagcaaaac tccagttaca tccaatagcc
      901 gatgcttcaa ttccagaaac tctccctaat tcataagcat atttcgcatc atttgttgca
      961 ccaattttta cttctacacc aacttcggta ccatctgtac aagctccgtt accacctgct
     1021 tctgtattag cagcaattaa taaaggaatt ttgctttctg tttgtaggat atagttttga
     1081 tcatatacct catcagcagt tcctggatta tatcgtacag ctccaatatg gtactgattg
     1141 accatactac ttaagtaatt tttatcacga ctagatccca tattaacaaa taattgacct
     1201 attttttctt ctaaa
//
LOCUS       DACDPS010000235         1214 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.246, whole genome shotgun sequence.
ACCESSION   DACDPS010000235 DACDPS010000000
VERSION     DACDPS010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.246"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            70..1026
                     /locus_tag="IWJ07_002891"
     CDS             70..1026
                     /locus_tag="IWJ07_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334387.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
     gene            complement(1080..>1214)
                     /locus_tag="IWJ07_002892"
     CDS             complement(1080..>1214)
                     /locus_tag="IWJ07_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9334388.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
BASE COUNT          427 a          179 c          207 g          401 t
ORIGIN      
        1 ttgacaggga atcctaaaaa cattcggctt tttttatata atctttttaa ctcaacagag
       61 ataagttttg tggaattgta tacgctacct ataattataa aaacagaaaa aatgttaaaa
      121 gatttaaaac agtattatcc tctgtctgct tatgaaaaaa agaaattatt ccacttttta
      181 gcaatcaacc tgttggctga acaagctgat tctttagata ttgctagcaa tcagtcgcta
      241 tttttttcgg atataggatt ttaccactta aaaggagtat taggagaaaa atccgttatt
      301 tttatcaata agacactctc ttggctttat cttcatgata aattggatca aaaatacttg
      361 agaaaaaatg aagatgaaac aatcgaatgg ttgaatgaac gattcattga aagttttgaa
      421 aatcagttta atctgttgcg agaagagacg agccaattga tccgaagaga gttaaccaaa
      481 atacatttta aacttttgca ttacactatt aataaagctg atgaatttcc acttgatgtt
      541 tcttttttta gagaaaacta tccagcattt tattgctatt tgatagagta tataaatgat
      601 ctaacgtcaa aacgcaaaga gttgcttcaa gataaatttt ttcttttttt cagttattta
      661 ttattattga tcaatcacat acccattcaa ctgatttctg aaccagtaaa gatcatcatc
      721 gatttttctt atggtgctgc ctataatcaa tttatcaaga aaaatttaag cttgtacatt
      781 aatctgaaca cggaagtgat agaggcagga aagcctgagt tcccggatgt tataatcacg
      841 aataggaata atctatatga agaaagtgca tctcaagtag tggtttggct cgatccgcca
      901 agagcagtag actgggggaa tcttacaaag attttgttga ctatccaaga agagaaatac
      961 aaagacagca aaattgacaa gcaaatagac gattttcctg aagaaatcat cgacaggatc
     1021 gaatagtgaa cttcttttag aaagtccgac tgctttttct aaaaatatta tttgtcaaat
     1081 taagcgagac aaaactttcc atttacgtaa ctcaaaaaaa cttctaatgg tgtttggtaa
     1141 tttagtgatt ttctagggat atggtttctt ttggacgcaa cggatgagat gaatccttga
     1201 ttgacttggt tgaa
//
LOCUS       DACDPS010000236         1213 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.247, whole genome shotgun sequence.
ACCESSION   DACDPS010000236 DACDPS010000000
VERSION     DACDPS010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.247"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..749
                     /locus_tag="IWJ07_002893"
     CDS             <1..749
                     /locus_tag="IWJ07_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP9334389.1"
                     /translation="IFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITEEKEQ
                     QKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKELYANK
                     IQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEPMDRI
                     KELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLDEIEK
                     VVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            821..>1213
                     /locus_tag="IWJ07_002894"
     CDS             821..>1213
                     /locus_tag="IWJ07_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9334390.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQDMLDYYYFEKNLSYNYV"
BASE COUNT          532 a          137 c          196 g          348 t
ORIGIN      
        1 aaatatttga taaaattaat aatttattca agaatgacaa tgctgaatta aggaagggtc
       61 ttttagagtt taaacttcaa gaactagaac ttcattactc ttatttagtg aaaattaccg
      121 aagaaaaaga acaacaaaaa gcaataaaag aacagatgat agaagaggaa aaagttcgta
      181 gagaaattga tcgtgaaaag aaacgtattg ataaagaaca acgtcaattc aattctgaaa
      241 tttccaaatt aatcgcatat atgcaaaaag ctaatgcaga tgtggaaaaa gaattatatg
      301 caaataaaat tcaagaactg gaagaaaaat taaaagaatt agaagttata cgtgaaaatg
      361 tattacaaag agaattaaat actagagccg gttatgtgta tgtaattagt aatattgggt
      421 cttttggtga agacatatat aaaattggta tgactagaag attagaacct atggatagaa
      481 taaaagaatt gagtagtgca tcggttccat ttgaatttga cgttcatgcg atgatttttt
      541 cagaggacgc tccgtctttg gaaactaaat tgcataatca ttttagaaaa caagaagtta
      601 ataaaattaa tcaaagaaaa gaatttttca aagtttcact agacgaaata gagaaagttg
      661 ttttagaaaa ttataatgga acagtaacat ttacaaaatt agctaaagct gaacaatata
      721 gacgatcttt agaattgtca aaagattaaa tatataaaaa gccttcgggc tttttctttt
      781 aacaattaat aggaacgtat attcgaaagg aatagaaata atgtggttag aaaaaataaa
      841 tgacaaaaaa tttaagtaca ccgaacgtta tacggatccc ttaactgaaa aaaagagaaa
      901 agtgtccgtt actttatcta gcaattcacg ccaagcttgg aatcaagcta acttactttt
      961 gaatgaaaaa atcgcagaaa aaataaaacg aaatgaagaa ttaccactaa cttttggaga
     1021 attaaaaact aaatgggatg aaaaatacaa gccaactgta aaagaaagtt cctatcgaac
     1081 aactcaggtt tatctatcat taatttcaaa atatataaaa gatgatgtac ttgtaaaaaa
     1141 cgttaatagt aatcttatac aagatatgct agattactac tattttgaaa aaaatttatc
     1201 atataattat gta
//
LOCUS       DACDPS010000237         1201 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.248, whole genome shotgun sequence.
ACCESSION   DACDPS010000237 DACDPS010000000
VERSION     DACDPS010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.248"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            151..1191
                     /locus_tag="IWJ07_002895"
     CDS             151..1191
                     /locus_tag="IWJ07_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAP9334391.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
BASE COUNT          484 a          173 c          199 g          345 t
ORIGIN      
        1 tagtacataa ttcattttat ttctgatttt aggtagtatt taattaaaaa acagttttaa
       61 tgcgtttgca ttttttattt atctttgcta ttttcgaaaa gagtaggtaa ttttacataa
      121 ataaaacaac gtgaaagtag aggatctaca atgactgatt ttaaattttt taaggcggat
      181 agagtttaca acgaattatt ttatcaattt ccaaaagtct ttattgtttc tgacgaatac
      241 aaaaaaatga aagattcaac taagattgcc tatatgcttt taaaagcaag attagagatc
      301 gcaatcagca aacggcaaat cgatgaagaa ggtaatgttt attttactta tacgacaaat
      361 gaactatgta gagtattaaa ctgccaaaaa caaaaagcga tagcaatcaa aaaagagttg
      421 gaatcctttg gtttattatt acaaaagcag atgggattta acaaacagtt agggaaaaat
      481 aatcctaata gactatatct agcagaatta aaagtctcag aaaatgatat ctacttactc
      541 gaaaaatttg atagagagaa tagggaaaac gttgataaat cagagggtat gaaaatcata
      601 cccaccctcg acgaaaaatc agacgctgaa tcccttgggg ctcaagaggg tatgaaaatc
      661 ataccgtgcc aaaacgttga taaatcagag ggtatgaaaa tcataccaga acttaataat
      721 aatatattag acactaatag acacaatata gacactgaaa aagaccgcct acaagatcaa
      781 ttgttgttag acaattttga gacaattatg acaaacgaca gcattgctac gtttgtccct
      841 gaacgagtat taaatttgat aaaaacattt tcttcaagtt acagtgaagc tcaaaaaacc
      901 gtccagacta ttcataatgc aaagaaaaaa gctgaaatag aaagtggtat ttcgatagtt
      961 tttgaagaac tcgatagtta ttatgtcaat gcagaacaag aattatacac gacactgtta
     1021 aaagcctatc aaaaattaaa aaccgaaaaa gtcgaaaata tccagaacct gatttttgtc
     1081 tatgtaaaaa attggtttat cgaaaaacca atagctgcta aagtatcaag tgaaaaacgt
     1141 ttgaattatg aaagctcccc aagcactatt acgaaagact ggttagagtg aaactttact
     1201 g
//
LOCUS       DACDPS010000238         1168 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.249, whole genome shotgun sequence.
ACCESSION   DACDPS010000238 DACDPS010000000
VERSION     DACDPS010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.249"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            10..1080
                     /gene="xerS"
                     /locus_tag="IWJ07_002896"
     CDS             10..1080
                     /gene="xerS"
                     /locus_tag="IWJ07_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAP9334392.1"
                     /translation="MYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKNYKLFY
                     EWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNNNLGLS
                     TSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARANAIEKK
                     LFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSEVVNIN
                     LEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKALFLAIY
                     QGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQLGHTS
                     TNTTTLYTHIDNAATIDALNAL"
BASE COUNT          468 a          194 c          180 g          326 t
ORIGIN      
        1 aatacagaaa tatatcaaaa aatagataca atcatacttc atatgcccga ttatataaaa
       61 gattttgttc aagatcgtga ggataaagat caatcacctc gaactacttt agaatatcta
      121 aaaaattata agctctttta tgaatggctt ctgagtgagt ctatcgttcc cgatacttca
      181 atcacctcga taagacagtt aactgcctat gatctaaact tgtacaaatc tcatttgaaa
      241 cgtcgcacaa aggaaaatgt aaaaaaagag acaacaaaaa ctaagctcaa agataataat
      301 aacttaggtc tatctacttc tactatcaat agaaatatca cagcattaaa ggttctattt
      361 aaatatcttt cgaaaagttc caataatcct ttaggaaaac catacttaga aaataatcct
      421 atggatcaag tagcaactat cacagataaa actacgttag ctgcccgtgc aaacgcaatc
      481 gagaaaaaat tatttctaga cgaagataca caaaattatc tagattatat tgccaatgaa
      541 tataaaaaaa ctttgagcaa acgagctttg atctattatc aacgagatgt agaaagagac
      601 ctggcgatca atgcattgat tttaggatct ggattaagac tctccgaggt tgtaaatatt
      661 aatttagagg acctatcttt agaaaagaat aatgtggtag taactagaaa aggaaataag
      721 agagacgcag taaatatagc agcatttgca atggaatatt tagctaatta tctagctatt
      781 cggaaagaac gatacaaagt aacagaaaac gaaaaagctt tatttctagc aatttatcaa
      841 ggagaagcca agcgtatatc cggtattgca atcgaacgga tggtagcaaa atattcaaaa
      901 ggattcagag tccaagtcag tccacataaa ttacgtcata ctttggctac tagactctat
      961 caacagacaa attcattggt attaactgcc caacaattgg gacatacaag tacaaataca
     1021 acaactttat atactcatat agacaatgca gctacaattg atgctttgaa cgctttgtaa
     1081 atgtagtaaa aaatagtata ataatgaagc ctgtctctaa aattagataa aagcccaact
     1141 ttaaaggcaa gcttcattat cattttta
//
LOCUS       DACDPS010000239         1118 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.250, whole genome shotgun sequence.
ACCESSION   DACDPS010000239 DACDPS010000000
VERSION     DACDPS010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.250"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(62..280)
                     /locus_tag="IWJ07_002897"
                     /pseudo
     CDS             complement(62..280)
                     /locus_tag="IWJ07_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(546..740)
                     /locus_tag="IWJ07_002898"
     CDS             complement(546..740)
                     /locus_tag="IWJ07_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334393.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
BASE COUNT          392 a          187 c          159 g          380 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattgta ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt attacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta tataatta
//
LOCUS       DACDPS010000240         1111 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.251, whole genome shotgun sequence.
ACCESSION   DACDPS010000240 DACDPS010000000
VERSION     DACDPS010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.251"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            136..>1111
                     /locus_tag="IWJ07_002899"
     CDS             136..>1111
                     /locus_tag="IWJ07_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HAP9334394.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTI"
BASE COUNT          357 a          233 c          199 g          322 t
ORIGIN      
        1 aattaaaacc atgtgaaact gcctcacggc agcttacaat tactttttca tcttcacccg
       61 ttaagtgatg aaaaaagaac ccctttctgc tatggttaaa gtgactaaac caaccaaaag
      121 aaaggagttc ttttcatgac tagcttacac aaaaaccaag taaaattcaa ttcaaatatc
      181 actatttctc atacaggtgg tcaattatcg agtgattcgg gtctcgtcct agtgaaagag
      241 ctgatgaaca tctttggttt ttctgaactg gctaagcaac acattcacat tgaagacgaa
      301 cgagcgtatt ttactcatga caacttatcc atacttgagc agttcattat gcaattaatt
      361 gctggttact cagctgattc tgcagctaat ctcctgagac aagatcctgt gtttaaagct
      421 gttttagata ggaaagaact cgcttctcaa tcttcacttt cccgattttt agatcgacta
      481 tctgaggaga atatccatga acttcaagct ttgaaccaag aacttatcga taaagcacgc
      541 ctcatccgta atgatacgga attaatcatt gatttagatt cgacccattc agatacattt
      601 ggtcaccaag aacaaacgaa ttataataca cactaccaaa cctatggtta ccatccattg
      661 gtagcttttg acggattgac tggtgacttc ttaaaagctg aactacgttc aggaaatcaa
      721 tatacatcaa aaggcgtaaa ggagtttctc acacctttat tagagcacta taatcactct
      781 ctaccaaaca ctgacatctt ggttcgtgga gacagcgggt tcgctacacc tggtgtgtat
      841 gattcgtgtg aatcaaaaaa gagtcattat gttattcgac tgaagaataa tcgtagacta
      901 ggtcaaatag ctgagaaatc agtactttat ggcgataatc aaaagtggga agaacgagaa
      961 gttcagtact tctcgaccac ttatcaagca caatcctggt cacaaagtcg tcgcgtgtgt
     1021 atacgctcaa cacgtgaagc gggcgaacta ctctttcgac atgaatttat cgtgacgaat
     1081 ctatcagaaa atgtttctcc tgataccatc t
//
LOCUS       DACDPS010000241         1035 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.252, whole genome shotgun sequence.
ACCESSION   DACDPS010000241 DACDPS010000000
VERSION     DACDPS010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.252"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..>1035)
                     /locus_tag="IWJ07_002900"
     rRNA            complement(<1..>1035)
                     /locus_tag="IWJ07_002900"
                     /product="16S ribosomal RNA"
BASE COUNT          218 a          324 c          234 g          259 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg ccttt
//
LOCUS       DACDPS010000242         1009 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.253, whole genome shotgun sequence.
ACCESSION   DACDPS010000242 DACDPS010000000
VERSION     DACDPS010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.253"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..848
                     /locus_tag="IWJ07_002901"
     CDS             <1..848
                     /locus_tag="IWJ07_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9334395.1"
                     /translation="EIADLIEKMYGHHYTPQTMSNITKSFTEEVTAFKGRELHDRYAA
                     IYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQER
                     GLKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVFRNISHKVRVNNRKEVCDDFKM
                     VYQASSKEVALEARGAFAKKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLI
                     ESFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLS
                     QPMEN"
BASE COUNT          329 a          195 c          202 g          283 t
ORIGIN      
        1 ctgaaattgc tgatttgatc gaaaaaatgt acggtcatca ctatactcca caaaccatgt
       61 ccaatataac aaaatcattt acagaagagg taacggcgtt taaagggcga gagcttcatg
      121 accgttatgc tgctatttat atggacgcaa cgtatattcc gttaaagcgg aaaaccgtcg
      181 ccaaggaagc tattcatatc gcagttggca ttcgcccgga cggatcaaag gaagtattga
      241 gctatgcgat tgcaccgact gaatccatca cgatttggga ggaaatttta ttagaccttc
      301 aagagcgcgg tttgaaaaat gtcctcctgt tcatcacgga tggcttaaag gggatggtag
      361 gagcgattag tcggttctat cccaaagctc gttttcaaca ttgttgtgta cacgtttttc
      421 gtaatatcag tcacaaagtg cgtgtcaata atcgtaagga agtctgtgat gattttaaaa
      481 tggtgtatca agcctcatct aaagaggtgg cgttagaagc acgtggtgct tttgcgaaaa
      541 aatggaaaac cagctatcca aaagtggttg aatcgattct ttcgaacgat cacttgctca
      601 ctttctatga tttccccttg gccatacgca agagtattta ttctacgaac ttgattgaat
      661 cctttaataa gcaaatcaag aaatacagcc accgcaagga acagttccaa aacgaagagt
      721 cgatggaacg tttcttagtc tcgtcttttg atacttacaa ccaaaaattc ctaggtcgca
      781 gtcataaagg tttccaacag gccgaaggcg agctcgaaca aatgctgagt caaccgatgg
      841 agaattagaa acaatctaca gtagggaaga accatttaca caaaattatt gacgctccca
      901 agctaaaact cctattaatc gatctactaa aaataaataa aaaaattctt ttcaatttta
      961 tttatggtat gacataaata aagtaaagtt attcttctag gttaatctt
//
LOCUS       DACDPS010000243          991 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.254, whole genome shotgun sequence.
ACCESSION   DACDPS010000243 DACDPS010000000
VERSION     DACDPS010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.254"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..824
                     /locus_tag="IWJ07_002902"
     CDS             <1..824
                     /locus_tag="IWJ07_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9334396.1"
                     /translation="KTMYNDFSPKEQAIADYILENPSKVSHSPISNLASELGIADSTF
                     FQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVFESSISTLND
                     TKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIATSHSTDYHIQ
                     LMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSSLAKLGDVVF
                     ISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHTISEIKE"
BASE COUNT          336 a          180 c          173 g          302 t
ORIGIN      
        1 tcaaaacaat gtacaacgac ttcagcccaa aagaacaggc tatcgccgat tatattttag
       61 aaaatccaag caaagtaagt catagtccta ttagcaattt agcaagtgaa ctgggcattg
      121 ctgactctac tttttttcaa tttacaaaaa aacttggtta taatggattt aaagatttta
      181 aaatggccat gttgatccaa gaaaatgatc tatctgctat ttctatccac gaaaatattc
      241 agaaaacaga tactgagctc actatggcgc aaaaggtgtt cgagtctagt atttcaactt
      301 taaatgacac aaaaaagttg ttacaacaaa aggatctaaa aaaagcggct actattatca
      361 atgaatctaa acgtgtctat tttttcggtg ttggcggttc agagattgta gcaactgacg
      421 cttatcataa atttttgcgt tcccctatcg caacttctca cagcacggat taccatattc
      481 aattgatgga agcttcttta ttgacggaaa aagactgtgc tgttttgatt tctcacacag
      541 ggcaatcaaa agaaacaatc catattgctg aaaccgtaag aaaaactgga gcaaaaacaa
      601 tcgttattac tagccaagct aattcttccc ttgctaaact tggcgatgtc gtttttattt
      661 ctatttctga agaaacagaa ttccgatctg aagctttagc ttcccgtatt tctcaactaa
      721 gcattttgga ctctctatat gtgattttga tgttttacaa cgaaaaagaa tcacgtcgtt
      781 ctatcgctaa agttcgacat actatttctg aaatcaaaga atagcaccat aacagaaaaa
      841 gcgaaaaatc cataaaacaa aaaaagccac taccaaatat ttggtagtgg cttttttggt
      901 tctgcaaggg tggtacagaa catcaatagt cggtgggtta gaacgtatgt catcgcatct
      961 ttggagaagg ttgtattggt attattcgag t
//
LOCUS       DACDPS010000244          979 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.255, whole genome shotgun sequence.
ACCESSION   DACDPS010000244 DACDPS010000000
VERSION     DACDPS010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.255"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            346..979
                     /locus_tag="IWJ07_002903"
                     /pseudo
     CDS             346..979
                     /locus_tag="IWJ07_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          332 a          181 c          178 g          288 t
ORIGIN      
        1 tagttatata tttaaatttt taccagaaag aaatgaacac cttcaattat ctaaatctcg
       61 aaaatggtta gatgtaatca tcaggaaaaa caatcttaaa cgaattacta ttcatggtct
      121 tagacacaca catgctagct tgcttttagc aagctagtgc aaatattaaa gatgtgcaag
      181 agcgtttagg ccactcttct attcaaatca ttatggatct atatattcat attacagaca
      241 aaagaaaaga agaaaccgta gcgcaattcg caaaatatag gctctataat ttatgggact
      301 gatgaatcaa aattgattct tcagtcccat tgttatttta ggtatatgat tcgtacattg
      361 aaagagttga aaaactatct tccgaagccg tcaaaaagga ccctgccaaa agtattgatg
      421 gtggatgaat ttcgttcgca cgcttcaatt gtggataaaa tgagctttat ttgtgcggat
      481 ggcgagactg gaaaactcat tgatattttg cctacacgaa aattgtctcg attaacacac
      541 tattttagtc gctgtgcaaa tcctgaagcg gtggaatatt tagtgacaga tatgaacgcc
      601 gcctacttcc aactgacaaa acgtgtctta ccaaatgcga aagtcgtcat tgattgtttt
      661 catatcatca aacatttgaa tcaggcgttc aatgattttc gtgtacaaga aatgaaaaga
      721 ttacaccagt tagggcaacg acgggaagcc gagaaagtga aaaagaactg gcgctttctt
      781 ctaaaaaacc gtcaaaaaat caatcactat gattacaaaa catggcgcag ttttcgtgca
      841 cctaaatacc cgctactcac tgaagcaatg atgattgatc gattgctggc attttcaacg
      901 cctttaaagg acgcctatac ttttttccat gagctgaccg aagcctttag agacaaggaa
      961 tcagacttat tttttacct
//
LOCUS       DACDPS010000245          943 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.256, whole genome shotgun sequence.
ACCESSION   DACDPS010000245 DACDPS010000000
VERSION     DACDPS010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.256"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            2..>943
                     /locus_tag="IWJ07_002904"
     rRNA            2..>943
                     /locus_tag="IWJ07_002904"
                     /product="23S ribosomal RNA"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DACDPS010000246          925 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.258, whole genome shotgun sequence.
ACCESSION   DACDPS010000246 DACDPS010000000
VERSION     DACDPS010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.258"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..869
                     /locus_tag="IWJ07_002905"
     rRNA            <1..869
                     /locus_tag="IWJ07_002905"
                     /product="23S ribosomal RNA"
BASE COUNT          242 a          197 c          282 g          204 t
ORIGIN      
        1 atgcaggtta cccgcgacag gacggaaaga ccccatggag ctttactgta gtttgatatt
       61 gagtgtctgt accgcatgta caggataggt aggagccgta gaaatcggaa cgctagtttc
      121 gatggaggcg ctggtgggat actacccctg cgttatggcc actctaaccc gcaccactaa
      181 tcgtggtggg agacagtgtc agatgggcag tttgactggg gcggtcgcct cctaaaaggt
      241 aacggaggcg cccaaaggtt ccctcagaat ggttggaaat cattcgaaga gtgtaaaggc
      301 agaagggagc ttgactgcga gaccaacaag tcgagcaggg acgaaagtcg ggcttagtga
      361 tccggtggtt ccgcatggaa gggccatcgc tcaacggata aaagctaccc tggggataac
      421 aggcttatct cccccaagag tccacatcga cggggaggtt tggcacctcg atgtcggctc
      481 gtcgcatcct ggggctgtag tcggtcccaa gggttgggct gttcgcccat taaagcggca
      541 cgcgagctgg gttcagaacg tcgtgagaca gttcggtccc tatccgtcgc gggcgttgga
      601 aatttgagag gagctgtcct tagtacgaga ggaccgggat ggacttaccg ctggtgtacc
      661 agttgttctg ccaagggcat tgctgggtag ctatgtaggg aagggataaa cgctgaaagc
      721 atctaagtgt gaagcccacc tcaagatgag atttcccatt tctttaagaa agtaagatcc
      781 ctgagagatg atcaggtaga taggtcagga gtggaagtac agtgatgtat ggagcggact
      841 gatactaatc gatcgaggac ttaaccaata ggttaaaaga aaagaactcg gaaaaaaact
      901 tatgtgactt caaatccagt tttga
//
LOCUS       DACDPS010000247          900 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.259, whole genome shotgun sequence.
ACCESSION   DACDPS010000247 DACDPS010000000
VERSION     DACDPS010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.259"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(200..748)
                     /gene="aac(6')"
                     /locus_tag="IWJ07_002906"
     CDS             complement(200..748)
                     /gene="aac(6')"
                     /locus_tag="IWJ07_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP9334397.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          254 a          196 c          130 g          320 t
ORIGIN      
        1 caaaaatgta gaaagccatt ttcacaaaat ttttcttact actcgaaact aagcgacttt
       61 ttcacaacct ctggtacacg gtgttccaaa agtagcttct tcggaaataa gttggaattt
      121 cacaaaaatt caagaagtaa ttttcgtaaa ttccttctta tttcttgaag ctgattactt
      181 ctgtcacaac ctctttttat tattgagaat ttggtcgagg aataatcgtt tttgccatcc
      241 aaatatccgg tttgtcccag ccatttgcat ttggtaagac acctacgatt ttataaccta
      301 atttttcata gaattcatac ggatgttcac gaaggttctg gatggaagcc actttatcaa
      361 atgtatgctc atacaggtcc gtttgactta acgttgttcc atggtctaaa tcatccgtac
      421 ctaaataaat cgtgattcct cctctggaag ctacttcttt ttctaagtaa ttgactaatc
      481 gagtacctat ttggttcttt cgtcgggagc tttctacaac taatggatgc aattcccaac
      541 ctgtgatacc gtattgaggg attgcaccaa taaatcctac taactcatct tggtctaccg
      601 ctgctaccgc gattcgttct ggattcatca tttcttctac ttcttctgcc gagctgtctc
      661 catattcttc cggccaagtc agtctcagta aatcagaaag ctgatctttc aatactggat
      721 tattacggtc aaattcactg attatcatac gtcttccccc ttgttcttgc ttattctaac
      781 agtccttttg aaaaaacgaa atgttctcgc tcaaaaatct ttagctacca atcagtatct
      841 ctattctttc attatttatc ttacttataa aataaaaaaa ttatacagaa acactttatt
//
LOCUS       DACDPS010000248          899 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.260, whole genome shotgun sequence.
ACCESSION   DACDPS010000248 DACDPS010000000
VERSION     DACDPS010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.260"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..890
                     /locus_tag="IWJ07_002907"
     CDS             <1..890
                     /locus_tag="IWJ07_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAP9334398.1"
                     /translation="IAYMLFKNEFRRALHEKWIDDDGLLYLEFTQADLMDKLNCYQGK
                     VKTILNELETYNLISVVKGEFNPKTKKNEKNKYYLLQPEISADDLFIQNSFDDDLSET
                     IDKSTSSRNAEIARREKTAETLDNTGNAEIAQDYYINKSLDTKRHLKDTETDQLQNQV
                     LLDNFVEIMKEDSINTFVPEKVLSLIKTFSNTYEEAQQTVTTIHNAKHKAEELEKTVV
                     VYEELESYGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFIEKAIAAKLAN
                     EQRETFNELPTVSTENWLE"
BASE COUNT          377 a          139 c          151 g          232 t
ORIGIN      
        1 aaatagccta tatgcttttt aaaaatgaat ttcgtagagc cttacatgaa aaatggatcg
       61 acgatgatgg tctattatat ttggaattta cacaagcaga cctaatggac aaattaaatt
      121 gctaccaagg caaagtaaaa accattttaa acgagttaga aacatataat ttgatttcag
      181 tcgtaaaagg agaatttaac ccaaaaacga aaaaaaatga aaaaaataaa tactatttac
      241 ttcaacctga aataagtgcc gatgatcttt ttattcaaaa ttctttcgat gacgatttaa
      301 gcgaaactat tgataaatca acctcttctc ggaatgcgga aatcgcacgt cgagaaaaaa
      361 cagctgaaac ccttgataac actggaaatg cggaaatcgc acaagattat tatattaata
      421 aatctttaga cactaaaaga cacttaaaag acactgaaac agaccaatta caaaatcaag
      481 ttttgctaga taacttcgtt gaaatcatga aagaagacag cataaacaca tttgtaccag
      541 aaaaggtatt aagcttgata aaaacatttt ctaacactta cgaagaagcc caacaaaccg
      601 tcaccacaat acacaacgca aaacataaag ccgaggaact tgagaagacg gtagttgtgt
      661 atgaagaact tgaatcttac ggaattgatg cggataaagg attatatatg acgttgctga
      721 aagcttatca aaaacaaaag actgagaaag taaataattt gcagaatttg atttttgtgt
      781 atgtaaaaaa ctggtttatt gaaaaagcta ttgcggcaaa gcttgcaaac gaacaaagag
      841 aaacatttaa cgaattacca acagttagta cggaaaattg gctagagtag ttgataaaa
//
LOCUS       DACDPS010000249          890 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.261, whole genome shotgun sequence.
ACCESSION   DACDPS010000249 DACDPS010000000
VERSION     DACDPS010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.261"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..888
                     /locus_tag="IWJ07_002908"
     CDS             <1..888
                     /locus_tag="IWJ07_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAP9334399.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          386 a          116 c          144 g          244 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt
//
LOCUS       DACDPS010000250          890 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.262, whole genome shotgun sequence.
ACCESSION   DACDPS010000250 DACDPS010000000
VERSION     DACDPS010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.262"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..839
                     /locus_tag="IWJ07_002909"
     CDS             <1..839
                     /locus_tag="IWJ07_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9334400.1"
                     /translation="YQLVETQAPTGYELDATPIEFEIERSQTAVVELTKANRLTPGGV
                     VLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAP
                     TGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDA
                     NGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIF
                     LTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKR
                     NI"
BASE COUNT          313 a          162 c          226 g          189 t
ORIGIN      
        1 catatcagct ggtagaaact caagcgccaa cggggtatga attagatgcg acaccgatcg
       61 agtttgagat cgaaagaagt cagacagcgg tggtagaatt gacgaaagca aaccgactta
      121 caccaggcgg tgtcgtgttg acaaagatcg atgaccaaag cggggaagtc ctgcaaggag
      181 ctgtttttga gttacaagat gccaatggaa ccgttctgca gtcaggtcta accacaggag
      241 cagatggaaa actggcgatt gacggcttag caccaggagt gtatcagctg gtagaaaccc
      301 aagcgccaac ggggtatgaa ttggatgcga caccaatcga gtttgagatc gaaagaagtc
      361 agacagcggt ggtagaattg acgaaagaaa atcgacttac accaggcggt gtcgtgttga
      421 caaagatcga tgaccaaagc ggagaagtcc tgcaaggagc tgtttttgag ttacaagatg
      481 ccaatggaac cgttctgcag tcaggtctaa ccacaggagc agatggaaaa ctggcgatag
      541 atggcttagc accaggagca tatcagctgg tagaaactca agcgccaacg gggtatgaat
      601 tggatgcgac accagtaact ttcaagatcg aaaaagaaca agaagtggcg atctttttaa
      661 caaaagagaa tagaaaagtt gctgatagct cagatataag aggaaacaat ccaacaagca
      721 gtggaaataa gcatcttcca aaaactggag aaaccatcat tagccagttt atattgtcaa
      781 tattgggtgt gctacttgtt ttcatcagta taaaatttag aaagaaacga aatatatgag
      841 ctaaaataaa aaattgaata agcatagttt ttaaaatcaa ctagtaccca
//
LOCUS       DACDPS010000251          848 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.263, whole genome shotgun sequence.
ACCESSION   DACDPS010000251 DACDPS010000000
VERSION     DACDPS010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..848
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.263"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..276
                     /locus_tag="IWJ07_002910"
     CDS             <1..276
                     /locus_tag="IWJ07_002910"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HAP9334401.1"
                     /translation="YEKVQIVDVNNGNRFETYTIAGERGTGMICLNGAAARCVSTGDK
                     IILMAYCELNENEVKDHRPKVVFVDDNNKVERVTSYEKHGRLSDIIL"
     gene            478..848
                     /locus_tag="IWJ07_002911"
                     /pseudo
     CDS             478..848
                     /locus_tag="IWJ07_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          339 a          117 c          162 g          230 t
ORIGIN      
        1 tatgaaaaag tacaaatagt tgatgttaat aatggaaata gatttgaaac ttatacaata
       61 gctggagaga gaggaacagg gatgatttgc ttgaatggag ctgcagctag atgtgtatca
      121 actggagata aaataatttt aatggcatat tgtgaattaa atgaaaatga ggttaaggat
      181 catagaccta aggttgtttt tgtcgatgat aataataaag ttgaaagagt aacatcatat
      241 gaaaaacatg gaaggttatc agatataatt ctttaggttt tgtaatgtta tttatatata
      301 gctagactat tattgaaata tttattgatt actaattgga tagatgtcaa taaagtagac
      361 acaaaaagag aagatacaga ctgtagacaa agtccatttt atgtgaatcg gctacactag
      421 actagacaga aaaaataagg tgtgtagaat aataaaaaac acactggagg atttttcatg
      481 tcaagacgtc aacgaagaac ctattcaaaa gaattcaaac aacaaatcgt caatctctat
      541 ctcgctggta agcctcgcgc agaaattatt cgagagtatg agcttacgcc ttcttctttc
      601 gataaatgga tgaagcaagc acaatcaacg ggctcattca aaaaaagaga caacttaaca
      661 ccagaacaag cagaattgat cgcactaaga aagaaaaata agcaactcga aatggagaat
      721 gatattttaa agcaagcggc gctgatattc ggacgaaaag acaagtaatt gatgccaaca
      781 agcataaata ttccatatca gcgatgtgca aaattctaaa tatttctcgt cagacctact
      841 attatcaa
//
LOCUS       DACDPS010000252          821 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.264, whole genome shotgun sequence.
ACCESSION   DACDPS010000252 DACDPS010000000
VERSION     DACDPS010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.264"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>821
                     /locus_tag="IWJ07_002912"
     CDS             <1..>821
                     /locus_tag="IWJ07_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK signal domain/LPXTG anchor domain surface
                     protein"
                     /protein_id="HAP9334402.1"
                     /translation="PEDKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDK
                     SDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVV
                     VTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEGEKVEVGGTVDLTDNVTNLPTLPEGT
                     TVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGE
                     KVEVGGTVDLTDNVTNLPTLPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGT"
BASE COUNT          327 a          141 c          187 g          166 t
ORIGIN      
        1 ccagaagata aacttccagt gttgaaagca aaagatggtt atacagatgc gaaatggcca
       61 gaggaagcaa ctcaaccaat tacagcagat gatacagaat ttgtatcaag tgcaacaaaa
      121 ctggatgata aatctgacgc tgacaaatat aatcctgaag gtcaaaaagt gactacagaa
      181 ttgaataagg aaccagacgc atttgaggga attaagaaca aagaagattt accaaaagat
      241 actaagtata cttggaaaga aaaagtagat gttagcgcag ctggaaataa aaaagggact
      301 gttgtagtga catatccaga tggatcatct gatgaagttg aagtagatgt tacagtgaca
      361 gacaatcgtt ctgacgccga taaatatgag ccaacagtag aaggcgaaaa agtagaagtt
      421 ggaggcacag tagatctaac agataatgta accaacctac caacattacc agaaggaaca
      481 acagtaacag atgtcactcc tggtggtacg attgatacca acacaccagg taattacgaa
      541 ggggttatcg aagtaacgta tccagatggt acgaaagata cagtgaaagt tccagtagaa
      601 gtgacagaca atcgttccga tgccgataaa tatgaaccaa cagtagaagg cgaaaaagta
      661 gaagttgggg gcacagtaga tctaacagat aatgtaacca acctaccaac attaccagaa
      721 ggaacaacag taacagatgt cactcctggt ggtacgattg ataccaacac accaggtaat
      781 tacgaagggg ttatcgaagt aacgtatcca gatggtacga a
//
LOCUS       DACDPS010000253          811 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.265, whole genome shotgun sequence.
ACCESSION   DACDPS010000253 DACDPS010000000
VERSION     DACDPS010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.265"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..672
                     /gene="tnpB"
                     /locus_tag="IWJ07_002913"
     CDS             <1..672
                     /gene="tnpB"
                     /locus_tag="IWJ07_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAP9334403.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DACDPS010000254          806 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.266, whole genome shotgun sequence.
ACCESSION   DACDPS010000254 DACDPS010000000
VERSION     DACDPS010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..806
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.266"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            1..806
                     /locus_tag="IWJ07_002914"
                     /pseudo
     CDS             1..806
                     /locus_tag="IWJ07_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332662.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          330 a           92 c          126 g          258 t
ORIGIN      
        1 agctttgagc caactgattc atttcagtat cttagcaaag atattgaaat tgaagatatt
       61 tataaaattt tagctcaaga gctatctaaa aatgttatta gtttgccaaa ggttttttat
      121 tttccagccg aaagaactgg tataaatgtc tttaagaacg aactaaatga aagtcgctta
      181 aaaacatatg ataccttgct gaatactgtg caatttacta atttaactaa ccaaaaaaag
      241 aaagctgccc tcaagtacga tctgttaaaa aagaatattg agctgttaat ggagacaacg
      301 gataatagtt cttatccaaa gccaatatcg gattatatta attatctgaa taacatgaaa
      361 aatagatatg aacagaaaaa tgtcaataat atagcatcat atattagaga tgagattatt
      421 atgggtaaat atgaaattga cgaaaaagat aattctgtaa gttttagaca aaagtttggt
      481 aaaaatagat atagaaaaga acgaattcct tttcacgttg tgtcttcttc gattaaatct
      541 ctatatggat tagattatta cttagaaaat tcaagtaaag aaggagacta tcttattatt
      601 gatgagccag aattgagttt acatccagaa aatcaggtta agttagcaaa agtaattaca
      661 gatatagttg agagtaaaat taagctaatt acttctaccc atagtgattt atttgtacgt
      721 gagcttacaa atattgttat tgaaaataaa ttaaatagta agaataataa aaaaataaat
      781 tgtctgataa aaatataggc atatat
//
LOCUS       DACDPS010000255          777 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.267, whole genome shotgun sequence.
ACCESSION   DACDPS010000255 DACDPS010000000
VERSION     DACDPS010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.267"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..446)
                     /locus_tag="IWJ07_002915"
     CDS             complement(<1..446)
                     /locus_tag="IWJ07_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9334404.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYVSVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT          233 a          136 c           96 g          312 t
ORIGIN      
        1 aactttctct tttcaaaaaa aatttgatat ttttttagat ttctttttag tctttgtaat
       61 actttataag ccacagaata actcacgtga tgctgtttag caaaattttc tactgagaca
      121 tatttttcct gaaagaaagc caataccatt ttcatagata aagaatccgt tatataacga
      181 gataacagag ctttgctaga acattttcca ttaatttcca gacatatatc caattcactg
      241 acggttactc gcaaattttt ctctagatcg aacttttgta aatcatgttt cagcttatcg
      301 atcgtttttt tcaagagaaa tgtcgatagc gacaactctt cttgaacatt cgatattgac
      361 tctgataaat ttgagcttgc ttctaaatat ttaagtaatg aataacttct gtgatcttct
      421 ttgcttaaaa aaaactcata attcattagt catcagctct ttctacgtat attactttcc
      481 atgcattcac caactagctg gggataaaac tgatttcctg gatccgagtc actgttgtca
      541 gcgattcttg tttgtataat atacatcgtc ctgtcttttt tttaacattt tggagagcgt
      601 tttatttggc ctttctcgcg atcaaacctg ttattagaat tatatcattt actagtatgc
      661 atttttatta ataaaatggt atattttata ttgttataaa aagagcccaa ccatcctaca
      721 ataataaaaa tttactattt gttatcttaa ccttactttt ctttctcttc ttttttt
//
LOCUS       DACDPS010000256          749 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.269, whole genome shotgun sequence.
ACCESSION   DACDPS010000256 DACDPS010000000
VERSION     DACDPS010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.269"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>749
                     /locus_tag="IWJ07_002916"
     CDS             <1..>749
                     /locus_tag="IWJ07_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAP9334405.1"
                     /translation="LNDELREIIEKQIERFNPQEPLFLLIDYFRVRFPTTDALAIIRD
                     VLQLKSNYMLYEDYGKYGYESKYVLGDINIMCSMQEHLGVLLELKGKGCRQMECYLLA
                     QERSWYDFMLDCMTAGGVMKRLDLAINDKAGILDIPKLKEKYKAGECMSYFRMQKDYS
                     GTEKCGSDLPKNTGETLYLGSTSSELYMCAYQKNYEQYVKNGTEIEDTEIKNRFEIRM
                     KNERAYYAVVDLLTYRDAERTAFSIINHYVR"
BASE COUNT          245 a          124 c          169 g          211 t
ORIGIN      
        1 attgaatgac gaattaagag aaatcataga aaaacagatt gagcgtttca atccgcaaga
       61 accactattc ttattgattg attactttcg tgtccgcttt cctactacgg acgcattggc
      121 gattatccgt gatgtattac aactgaaatc taattacatg ctttatgaag attatggaaa
      181 atacggatat gaaagcaaat atgtacttgg cgacatcaat atcatgtgtt ccatgcagga
      241 gcatttaggt gttttattgg aactgaaagg caaaggctgt cggcaaatgg aatgttatct
      301 gctggcacag gaacgctcat ggtatgactt catgctggat tgtatgacgg ctggtggtgt
      361 gatgaaacgg cttgatttgg ctatcaatga taaagcagga atattggata ttccaaagtt
      421 aaaggaaaaa tacaaggctg gcgaatgtat gtcctacttc cgtatgcaga aagattacag
      481 cggtacagaa aaatgcggta gcgatttacc aaagaataca ggagaaacct tatatctcgg
      541 ttcaacaagt agcgaattat atatgtgtgc ttatcagaaa aattatgagc agtatgtcaa
      601 gaatggcaca gaaattgaag atacggagat taagaaccgt tttgaaatac gcatgaagaa
      661 cgaacgagcc tattatgcgg ttgtagattt actgacctat cgggacgctg aacgcaccgc
      721 tttttctatc atcaatcatt atgtccgct
//
LOCUS       DACDPS010000257          747 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.270, whole genome shotgun sequence.
ACCESSION   DACDPS010000257 DACDPS010000000
VERSION     DACDPS010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.270"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            14..715
                     /locus_tag="IWJ07_002917"
     CDS             14..715
                     /locus_tag="IWJ07_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP9334406.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT          260 a          142 c          131 g          214 t
ORIGIN      
        1 agaggacttt ttaatgcgtg ttaataaaat cgattttcac ttttcaaaac tcagatatca
       61 acctgatttt gaacatttct cggatgaaga gttccaacag atcaaggaac atactgttct
      121 tcgttcttat aaaaaaggac agatcctttt cgacgatggt gatcccagac aacggttgta
      181 tatcttgacc aaaggactcg ttcgtttaga acgttatgat gaaagtgcaa cttattacta
      241 ctatgactat gtgaatacaa ataacctttt tcctatgtca ggaatgtttg gagaagagac
      301 ctacacttat acagctcaag cgatgactga tattgaaaca ttctaccttc ctgtttccta
      361 ctatgaaaaa ttagcacgaa aaaatcccga acagttgatc tatatgatac aaaaaatttc
      421 taaagtagtt gaaatacatg aattgcgtat ccaaatcgga ttgacctcta gcgcctttga
      481 ccgggtaaaa caaaatttat tcattttgaa agaagaattg ggcgtaccaa caaaagatgg
      541 tcatatcacc attccttatc cgatcacctt aaaagaatta gcagtgaata gcggcacgac
      601 acgtgaaact gctggacaag taatcaaaca actacgtgat tccggactgt tagattatga
      661 caaaaagcgt tttactttct gtaagacagc tactactcca gctatgagag aataacgcga
      721 acaaaaataa gcagtgaccc tgatatt
//
LOCUS       DACDPS010000258          735 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.271, whole genome shotgun sequence.
ACCESSION   DACDPS010000258 DACDPS010000000
VERSION     DACDPS010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.271"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            101..631
                     /locus_tag="IWJ07_002918"
     CDS             101..631
                     /locus_tag="IWJ07_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334407.1"
                     /translation="MISDNKWEINLKRFISEYIYTIETKSSFSLNEDSDPLKKYTIQC
                     KKNKDEKLFVIRPDLMKSNTHYIKEKPKDCDYIIISEANKTVFLIELKSSAQTSPAKE
                     ISEQIDGGYKWLQHLSFLTKYDFTEFSYKIIKVCCKFEARQDRKNLFKPNEYGIYNMN
                     GPKLDLRELYRKCNDL"
     gene            complement(626..>735)
                     /locus_tag="IWJ07_002919"
     CDS             complement(626..>735)
                     /locus_tag="IWJ07_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9334408.1"
                     /translation="NIVKDLNEGVSISKIAKNYNVTKQTIYRIKKDLNL"
BASE COUNT          296 a           85 c          111 g          243 t
ORIGIN      
        1 aaatttctaa catcagttat tatgataatt ttgatgatgt caattatgat ttacaggaaa
       61 gatataatga tttaatcgaa gaattagagg agagaggaga atgatttcag ataataaatg
      121 ggaaataaat ctcaaaagat ttatttcaga atatatatat acgattgaaa ctaaatctag
      181 tttttctctt aatgaagaca gtgatcctct aaaaaaatac actattcaat gtaagaaaaa
      241 taaagatgaa aaattgttcg taataagacc agatcttatg aagagtaata cccactatat
      301 aaaagaaaaa cctaaagatt gtgactacat catcataagt gaagcaaata aaacagtatt
      361 tctgatagaa cttaaaagct cagctcagac ttcccctgca aaagaaatta gtgaacaaat
      421 tgatggtgga tataagtggt tacaacatct tagttttcta acaaaatatg attttactga
      481 attcagttat aaaattatta aggtatgttg taaatttgag gcaagacaag atcgtaaaaa
      541 tctttttaaa cctaatgaat atggtattta taatatgaat ggtccaaaat tagacttaag
      601 agagttgtat agaaagtgta acgatctata aattcaaatc ttttttaatt ctatatattg
      661 tttgtttagt aacgttataa ttctttgcaa tctttgaaat agagacgccc tcattcaagt
      721 ccttaacgat gtttt
//
LOCUS       DACDPS010000259          716 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.272, whole genome shotgun sequence.
ACCESSION   DACDPS010000259 DACDPS010000000
VERSION     DACDPS010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.272"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            51..704
                     /locus_tag="IWJ07_002920"
     CDS             51..704
                     /locus_tag="IWJ07_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9334409.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
BASE COUNT          301 a           59 c          133 g          223 t
ORIGIN      
        1 ccataatatg ctgtataata tctagtgtat aggcttaagt ggaggtatat atgaaaatat
       61 taactgttga agatgataat atgataagag agggaataag tgaatatctt tcagaatttg
      121 gatatactgt tattcaagct aaagatggaa gagaagcctt gtcaaaattt aatagcgata
      181 taaatttggt tatcttagac attcagatac cttttataaa tggtttagaa gtgttgaaag
      241 aaattagaaa gaaaagcaat ttaccaattc taatcttgac tgcatttagt gatgaagaat
      301 ataaaattga tgcatttact aatttagtag acggatatgt agaaaagcca ttttcattgc
      361 cggtcttaaa agctagaata gattctttaa ttaagaagaa ttatggacat ttagagaagt
      421 ttgaatataa ggatctatcg gttaatttta atagctatac agctaaaata aatggtgaag
      481 aaatagatgt aaatgcaaaa gaactagaag tattaaaatg tttattggat aataatggac
      541 aagtgttaac aagaatgcaa attattgatt atgtatggaa agatagcgaa gaaactcctt
      601 atgatcgagt aatagatgta tatataaaag aacttagaaa aaaattacaa ttagactgta
      661 taactactat aagaaatgta ggatataaat tggagagaaa atgaaaaaat taaaga
//
LOCUS       DACDPS010000260          709 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.273, whole genome shotgun sequence.
ACCESSION   DACDPS010000260 DACDPS010000000
VERSION     DACDPS010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.273"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..100
                     /locus_tag="IWJ07_002921"
     CDS             <1..100
                     /locus_tag="IWJ07_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9334410.1"
                     /translation="KRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            complement(223..>709)
                     /locus_tag="IWJ07_002922"
     CDS             complement(223..>709)
                     /locus_tag="IWJ07_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_465591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HAP9334411.1"
                     /translation="PVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSR
                     GMGGIGLPGDLSSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGD
                     GKYEYTIYSSCCNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYI
                     N"
BASE COUNT          259 a          113 c           93 g          244 t
ORIGIN      
        1 taaacgaaat aatattccta gaaaatcact ggattatcga acacctttgg aagtattttt
       61 gagttacgta agtattgatg atctgtctaa cttaatttga caattaagaa gttaaaaaca
      121 ttacaaaaaa gacaaggata tcacatcaag aaaaaatgtt ttgaaaatat ctttgccttt
      181 ttgtattata tctatattaa ataatcaatc acaacacatt aactaattta tatatttaat
      241 ttgttgtttt tctataattg gataactaat taacttatga ctatctaagt cttctttatt
      301 catatcaatt gcagtaattt gactgtcttc atatgttcga taataataga ttcctttgtc
      361 aacattgcaa caagaagaat aaattgtata ttcatatttt ccatcaccaa catcacacaa
      421 acctttttgt tgttctactg aacctaagat atggaaaaat tgactaatac tttctgactc
      481 tgaatctcca gatacagaat tcagcttcgt aaaagtcgct ttaacaaaac gagatactga
      541 ggataaatct ccaggcaagc ctatccctcc cataccgcgg ctataggcat tcaaacttat
      601 ttgatttgaa aaattatttt taggagtttc actcgataag acacgataat tgtttaaatt
      661 aaataattga tagtcaaatg aaggattatt ggtaagaacg cccacaggg
//
LOCUS       DACDPS010000261          704 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.274, whole genome shotgun sequence.
ACCESSION   DACDPS010000261 DACDPS010000000
VERSION     DACDPS010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.274"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(281..478)
                     /locus_tag="IWJ07_002923"
     CDS             complement(281..478)
                     /locus_tag="IWJ07_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9334412.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          247 a          125 c           95 g          237 t
ORIGIN      
        1 attgtgatcc acctcttttg tttattagtt taaaccatca aaccattttt aataaaatat
       61 gtaaattcct ttgctcaaaa aagtagcttc tttacgattc gacaatttca aataaagcag
      121 gtaaaaactg gctgtcagaa aatagccaaa ctgaagcaaa aaataatgtt aaaattaatt
      181 aatgaacgaa aaaaatccct cacctaatat tacggtgagg aattttttct accgacataa
      241 acaaaaaaac cacctcatat gaggcggtta acgttcaaca ttattttacg atatttactg
      301 cttgaggacc acgttgtcct tcttcaatat cataagtaac tgcttggcct tcatctaaag
      361 acttaaagcc ttctccttgg attgctgaga aatgagcgaa tacgtcattt ccatcttcac
      421 cagtgatgaa accaaaacct ttatctgagt taaaccattt tactgtaccg ttattcatat
      481 atatttcctc ctgatacgta tataatacgt gttttattgc aattaatatt acttggtaaa
      541 aggagttttt atagtgtgaa tatatcgctc agattacgtt tcaaattaga ttacttattt
      601 actataacac ggatgtaatg ctaacacaag gataatcact cgatatacgg tttaaatatt
      661 attatttgtc aaattaagcg agacaaaact ttccatttac gtaa
//
LOCUS       DACDPS010000262          689 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.275, whole genome shotgun sequence.
ACCESSION   DACDPS010000262 DACDPS010000000
VERSION     DACDPS010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.275"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            82..>689
                     /locus_tag="IWJ07_002924"
     CDS             82..>689
                     /locus_tag="IWJ07_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HAP9334413.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRK"
BASE COUNT          230 a          119 c          135 g          205 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa cccattggac ggctcaaagt tattttgttc aacccagaca ttcaatcgcg
      421 aatcatgtca gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaaatt gtcaggtatc tttgaccacc gttattcgta cgttgaaaga gtttaaaagc
      541 tacttaccaa agcaatctaa gaggattctc cccagagtat tgatggttga tgaatttcgt
      601 tcgcacgctt ccatagaaga taaaatgagc tttatttgcg cagatggcga aacaggacaa
      661 ttaattgatg tcttgcctac gcgtaaatt
//
LOCUS       DACDPS010000263          689 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.276, whole genome shotgun sequence.
ACCESSION   DACDPS010000263 DACDPS010000000
VERSION     DACDPS010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.276"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            350..>689
                     /locus_tag="IWJ07_002925"
     CDS             350..>689
                     /locus_tag="IWJ07_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9334414.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYSFEEKIP
                     HFSTFGKNYVR"
BASE COUNT          224 a          128 c          113 g          224 t
ORIGIN      
        1 tctttaaaag aaattatttt cgcttcattt tattataaaa aggctaggaa ccttgatgtt
       61 atcaggttcc tagcctttat ttaagtatta ccccacagac ccttccatat agatttttaa
      121 attacacact agtttaaact aaaacaaaca aggaaacact taccatttaa tcataaaatt
      181 agctcatatg aatatattag aatgtaaaat agactggtca aaatctggtc aaaaaagagg
      241 actaatacag gtagtcctca gactgtagac aaagtccatt ttatggatga tatctacagt
      301 cttttctttt ataatagatt tatgaaaacg aatgcaagga gtgatagcta tgatgtctaa
      361 aaacaatacg aatggtagaa atcaatttgc gatgcttacg attgatgatc tagttcctca
      421 agatcatctc gttcgaaaga ttgatgccgc gcttgatttt gaatttattt atccaatcgt
      481 cgaagccact tactccgatt taggtcgccc aagtatcgat ccagtgattc tgatcaaatt
      541 ggtgtttatt cagtatttat tcggtatccg ttccatgcga caaaccatta aggaagtcga
      601 taccaatgtc gcttaccgct ggttcttagg ctactccttc gaggagaaaa tcccccattt
      661 ctcaactttt ggaaagaatt atgttcgcc
//
LOCUS       DACDPS010000264          658 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.277, whole genome shotgun sequence.
ACCESSION   DACDPS010000264 DACDPS010000000
VERSION     DACDPS010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.277"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(198..518)
                     /locus_tag="IWJ07_002926"
     CDS             complement(198..518)
                     /locus_tag="IWJ07_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334415.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
BASE COUNT          203 a          128 c          115 g          212 t
ORIGIN      
        1 gctctgatgt tcttctttaa actaaaaaaa ttattctttc tgactactta tcggttctgt
       61 ttcaacggtc gaggctgggg aagattcttt agtaggtctg atgttctcat ggtatttctg
      121 atagcctatg acagttacca gaagaacaag gcaaaccaat acgatcaacg agattagttt
      181 ttcttttttg ctcatatcta tacctcataa ataacaggat gatgtccatc atatttctga
      241 gtatgagtaa aagcaaaagc tgcagctgta gaatgtaaga aatgccagca actttcataa
      301 tcacaggtcg gacagtacca aaagtaacca aatcttggct gcaaagaccg ttcttctgtt
      361 tcaggaatcg cagaatcaag atctgttgtc tgtacataaa catttttgta cagacgtgtg
      421 cctgtaatag gagaccgctg tgtttcttca gcaaaaactg gctgacttgt taaacttagg
      481 gatagaaaca gcaaacacca taacagtctt tttttcataa agacctcctt atcgataagt
      541 ttttcccaat taaaatcata tctcaagaac gcccgttatt cacattttgt tcataaatga
      601 tgttttttgt atcataatcg tcaaaaagag gtcgtgacca cgtcacaacc tctttttt
//
LOCUS       DACDPS010000265          657 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.278, whole genome shotgun sequence.
ACCESSION   DACDPS010000265 DACDPS010000000
VERSION     DACDPS010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.278"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            167..>657
                     /locus_tag="IWJ07_002927"
     CDS             167..>657
                     /locus_tag="IWJ07_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP9334416.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGV"
BASE COUNT          251 a           95 c          129 g          182 t
ORIGIN      
        1 aaaaagccat gaaaaacgaa tagttagcgc ttaccgatgc gttttgtgat aaaaaaattt
       61 taaaatattc aatgaaaaca aagcatatat tttgctttaa aaccaaatga ggaatattct
      121 agatgtagaa caggttagaa aaagattaga aaaaggagtg attttgatgg agaagagata
      181 cggcgtagtt cctgtggtaa ctgcttcaga cgaaaattat gctccttact tgagcgtgat
      241 gatcgcaact gcattagaaa attgcaataa aacaagacgc ataaaatttt atgtgattga
      301 cgatggtcta tcagaatata gtaaagaagg attggaagaa acggtaaata aatattcaag
      361 taatgcaagt atccaattcc tgacagtgga aaaagatatc tacgaagact tcctagtcag
      421 tgaccatatc acaactaccg cttacttaag aatttcttta ccaaacttat tagctaaaga
      481 ggattataag aaagtccttt acttagattc agatgtgtta gtactagacg atatcgtaaa
      541 actatatgat gagccgttaa atggaaaaac aatcggcgct attatcgatc caggccaagt
      601 gaaagctttg gaaagattag gaatcgattc cgatgacttg tacttcaatt caggagt
//
LOCUS       DACDPS010000266          651 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.279, whole genome shotgun sequence.
ACCESSION   DACDPS010000266 DACDPS010000000
VERSION     DACDPS010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..651
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.279"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            132..503
                     /locus_tag="IWJ07_002928"
     CDS             132..503
                     /locus_tag="IWJ07_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334417.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT          200 a          111 c           96 g          244 t
ORIGIN      
        1 cattattttg ataataatga ttatttattt aaaaatacat tagataacat ttgttacgac
       61 acgaaaaagc ttcacaaaca aatatcgtta ttttaaaatg agggcgaact cacacaatta
      121 ggaggaatag tatgccattt attgtttact tttttctttc attactcgtg atctatattc
      181 cattaccaac atttttcctg ctcgtcaatc gtctagcaga gcaacatgat acgattgctg
      241 ctcttacgaa actttgttta cttctatttg tttgtctaga ttactttacg agctattatt
      301 ggctaaaaca gacaagagta aaaaagagat attatttctt ccttgtcttt ttcaatctct
      361 ttgagtttct gatccattgc tttttacttc atcagtatca tacgatccat ttgtggatca
      421 tctgggtttt caaagtggtc gtattatttt gtatgttcac ctttcctatc tcaagaaaat
      481 ttagggaggc attgttttat taaagaagct tctctgtatc aataaagaca catttaagga
      541 aaaactttta aaaatctaat tgggaatttg cccgcataaa taagaggctg tgacaaaagt
      601 cttgtcacag cctcttattc cgtataaaca gtgttcaaaa gcttgcatct t
//
LOCUS       DACDPS010000267          651 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.280, whole genome shotgun sequence.
ACCESSION   DACDPS010000267 DACDPS010000000
VERSION     DACDPS010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..651
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.280"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..156
                     /locus_tag="IWJ07_002929"
     CDS             <1..156
                     /locus_tag="IWJ07_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HAP9334418.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
     gene            175..>651
                     /locus_tag="IWJ07_002930"
     CDS             175..>651
                     /locus_tag="IWJ07_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9334419.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFA"
BASE COUNT          287 a           49 c          114 g          201 t
ORIGIN      
        1 gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat
       61 tcagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt
      121 ccagtggatg caattagtta tggagtaaga aaatagagag gagtagttag gaaagtgata
      181 aaaaatgcta tagcatatat tacaagaaag aaaaatagaa ctttaataat ttttatcata
      241 ttaactatag ttctttcttg cttgtattct tgtttaacaa taatgaaatc aagcaatgaa
      301 atagaaaagg ctttatatga aagttctaat tcttcaatat caataacaaa aaaagatggt
      361 aaatatttta atgttaatca atttaaagat attgaaaaat taaaagaaat tgaagaaaaa
      421 ataattcaat atgatggatt agcaaaatta aaagatgcta aagtagttag cggagaacaa
      481 agaataaata gagaagattt atctgacgaa tttaagaatg ttgtttcact cgaagctaca
      541 aataatacta aaagaaatat tttatttagt agtggagtat ttacaattaa agaaggtaaa
      601 aatatagaag aaaatgataa gaattcaatt attgttcatg aagaatttgc t
//
LOCUS       DACDPS010000268          641 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.282, whole genome shotgun sequence.
ACCESSION   DACDPS010000268 DACDPS010000000
VERSION     DACDPS010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.282"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>641
                     /locus_tag="IWJ07_002931"
     CDS             <1..>641
                     /locus_tag="IWJ07_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9334420.1"
                     /translation="LDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNIT"
BASE COUNT          250 a           58 c          108 g          225 t
ORIGIN      
        1 ctagatattg aaaaaagtgg aaaaataaaa agtcataaat ttaaaattat agggatcttt
       61 tctggtaaaa aacaggaaac atatacagga ttatcttctg attttagtga aaatatggtg
      121 tttgtagatt attcaacaag ccaagagata ttaaataatt cagaaaataa tgaaattgca
      181 aataaaattt taatgtattc tggtagtgca gaatctacag acttagcctt aaacaaattg
      241 aaagagctta aaattgatga gtcaaagtat tttgttgaaa aagattctaa tgcgtttgaa
      301 gagtctttag agtcagtgag tggaataaaa tatataataa aagtaatgac ttattctatt
      361 atgttaggtg ggatggttgt tctttcatta atcttgattc tatggttaag ggaaagaatt
      421 tatgaaatag gtatattctt atctattgga acatctaaga tacaaattat aatgcaattt
      481 atattcgagt taatattcat atcgatacca agtataatat cttccttatt tttaggaaat
      541 gtattactaa aagtaattgt agatggattc attaactcag aggattcaat gatttccggt
      601 ggaagtttaa taaataatag tagttttatg tcaaatataa c
//
LOCUS       DACDPS010000269          625 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.283, whole genome shotgun sequence.
ACCESSION   DACDPS010000269 DACDPS010000000
VERSION     DACDPS010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.283"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            87..572
                     /locus_tag="IWJ07_002932"
     CDS             87..572
                     /locus_tag="IWJ07_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334421.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
BASE COUNT          194 a          114 c          108 g          209 t
ORIGIN      
        1 ggaaggtttt ttcattttaa accatctgct tttctttgtt taagtgatat actgaataaa
       61 gataatctat tttaggagga gctatcatgc ataaagggat ttacgcaggc ttgttgggaa
      121 ctgtaatact tttttctgcc tgtggatata aaacaaagaa ttctgttaaa gaatcttcag
      181 cagccatttc aacaactgag gttggttcaa ctaaaagtac ttctactact tccattacta
      241 atacttctac tggtaatgta gattcgcaac atagccaatc aagtattcag tcgtttagca
      301 tttcttcaac atctgaaatg caagaaagca ctgtaccttc tcttccatta gaaaactctg
      361 acaaagcact tctatttctt attgcccacc gttccgaatt acaaagtgac gatattgtta
      421 ttagctttta tcaaaaaatt gatcgagatt atttgttcac agcttccagc aaacaaatcc
      481 gttcccaagg aggatctgga agcgttggtt tttatcgagt gtcacccgaa ggatcgatta
      541 cgatgacaga tgcaaatgga acaccatttt gattagatga tattatacgg atttacagta
      601 ctagcaagtc attattggta tcatt
//
LOCUS       DACDPS010000270          616 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.284, whole genome shotgun sequence.
ACCESSION   DACDPS010000270 DACDPS010000000
VERSION     DACDPS010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.284"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            95..565
                     /locus_tag="IWJ07_002933"
     CDS             95..565
                     /locus_tag="IWJ07_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAP9334422.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          222 a          108 c           93 g          193 t
ORIGIN      
        1 ttattgtctg aaaagagttc taaacgggaa aacaaatagc tagttttcag tttagcgctc
       61 tttttattat ataaaataga aaggagaaaa ctttatgtct atttataatt ttgaaactac
      121 tttagaagac ggcactgtct attcattgaa aaaatatgca ggcaaaccga taatcattgt
      181 taacactgca acaaaatgcg gtctagcacc tcaatttaaa caattagaag aactttacca
      241 agcttatcag acacaaggtc tggttatctt aggctttcct tccaatcaat ttaaacaaga
      301 gctagattca gcgactgaag cagcagaagc ttgtaaaacc acttatggcg ttaccttccc
      361 tatgcatcaa ttgattgacg taaatggaga aaatacagat cctcttttta cttatcttaa
      421 aaatgaagct cctggtactg tcactaaagc aataaaatgg aattttacta aatttttgat
      481 tgatagaaat ggcaaagtaa tcgaacgatt tgcaccacaa acttctccat tgaaaatgac
      541 cgctgcaatt gaatccgttc tttaacttaa taaaaataaa aaaagatgaa ctatttctag
      601 ttcatctttt tgcctt
//
LOCUS       DACDPS010000271          609 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.285, whole genome shotgun sequence.
ACCESSION   DACDPS010000271 DACDPS010000000
VERSION     DACDPS010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.285"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>609
                     /locus_tag="IWJ07_002934"
     CDS             <1..>609
                     /locus_tag="IWJ07_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP9334423.1"
                     /translation="EYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISF
                     VLSDNLLITCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRI
                     DALDKAARKTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQL
                     LYDVHLRQRQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMK"
BASE COUNT          226 a           99 c          117 g          167 t
ORIGIN      
        1 ccgaatatcc ggaagaagtt tctcggatgg agcgccttta tgagacgaaa ctagagcatc
       61 cattttcttt ggtagtgatt aatctagatc agtcagataa tcgtatcgaa aaaaaattaa
      121 cgccaatatc ttttgtactt tccgataatc tcttgattac ttgtatcgat agaaaaaccg
      181 aatttgtgga tcggctgctg acaaagcatg gaaatcattt aaactcgttt gaaaagatca
      241 tcacgtatac attattagat atttacaccc attacgtgaa agaactaaga gagatgaaaa
      301 agcgaattga cgctttggat aaagcagctc gtaaaacaac ggaaaatgaa gaactgtata
      361 aacaggcaga tcttgaaaga gacatcgtat atatcgatca taccttaaga gatcaacggg
      421 aaacaatgga tcaattatgg aattctgaaa ctttcatcaa acgtttagac gatgaacagc
      481 tactctatga tgtccatttg agacagcgac aaacagaaaa aatgatcgag atctatcgag
      541 atttattaga gagtattggt gatctattca acgggatgat ggacaataat ctaaatcata
      601 tcatgaaat
//
LOCUS       DACDPS010000272          601 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.286, whole genome shotgun sequence.
ACCESSION   DACDPS010000272 DACDPS010000000
VERSION     DACDPS010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.286"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>601
                     /locus_tag="IWJ07_002935"
     CDS             <1..>601
                     /locus_tag="IWJ07_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HAP9334424.1"
                     /translation="YEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGW
                     KKLFGKESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAG
                     KTVDSEEAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVE
                     KEPLLTSAEMTAKWESYLLKIGEQKGTQATFLANIQKFVS"
BASE COUNT          242 a          102 c          138 g          119 t
ORIGIN      
        1 tttatgaaca agtgttaaaa acaacggtgg ccatgtttgc ggaaaagtat acctatgaag
       61 aaacaacgat cttgacgcaa gttcagcagc ttcagctaaa gacagtcgga aaagtaccag
      121 tagatctagg ttggaaaaag ctgtttggca aagagagcga cagaaaagaa aaggaagaag
      181 aaccgctact tcctaaagtg acaaaaggag aagcggtgac ggtagatcta caagtccttg
      241 aaaaagaaac gaagccacca caaccttata ccgaaggtac gttgatcaca gcgatgaaaa
      301 cagctggaaa aaccgtggat agcgaagaag cacaatccat attgaaagaa gtagaaggaa
      361 tcggtacaga agcaacacga gcaaatatta ttgaaacttt gaaacaaaag gaatatatca
      421 aggtagaaaa aaataagctt gtggtgacaa ataaagggat tttactttgc caagccgtgg
      481 aaaaagagcc gctgctaaca agtgccgaaa tgaccgccaa atgggaaagt tacttattaa
      541 agattggtga gcagaaagga acacaagcta ctttcttagc taatatccaa aaatttgtct
      601 c
//
LOCUS       DACDPS010000273          596 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.287, whole genome shotgun sequence.
ACCESSION   DACDPS010000273 DACDPS010000000
VERSION     DACDPS010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.287"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(8..367)
                     /locus_tag="IWJ07_002936"
     CDS             complement(8..367)
                     /locus_tag="IWJ07_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP9334425.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT          205 a          112 c           76 g          203 t
ORIGIN      
        1 aacagattta cgaagcgtct gatgagtcaa aatagacttg gacattgcta tctaaaatac
       61 cactttcatt ctttaaacga attgtttgat ttgatggagt tacctctaat actaaatgcc
      121 cattaataaa ggtagtatcg tattcagatg ttttaactgt tgcttcttcg caaggtattg
      181 ttaaatcata tgctggaata taatgttgtg tggaaactaa taaaaaatga tcaccaatca
      241 tacagcatcc ctctgctgaa gaaaattgat tagagccatc atttaaagtt aacaagaatc
      301 catcctttaa acgaggtact tgcttaaata cttctggttg tacttctaaa aataaattat
      361 cgctcatttt cacgaccacc atcgacttcc acagttgaaa ttgacatttt atttaacaca
      421 cctattcccc cttaactata actcaattct atataaaaaa tattctattc tcaaataatt
      481 gaacttttca aatcgccaat ttacttcggc tttatcagag ctcattcata tcaagagcga
      541 ttaacgctgt aaaaattaaa ccgaagatct gataaatttt ctctattttc tacaat
//
LOCUS       DACDPS010000274          592 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.288, whole genome shotgun sequence.
ACCESSION   DACDPS010000274 DACDPS010000000
VERSION     DACDPS010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.288"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            72..>592
                     /locus_tag="IWJ07_002937"
     CDS             72..>592
                     /locus_tag="IWJ07_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP9334426.1"
                     /translation="MEIFGFYDTAFHINRALSLESIFSSPINFETFRSYGMQVNNFYP
                     WLTLYPLFLLIKFTNLAIGYNLFLYIVTLITLFICHYVMYEITKKHVTSSFFAIIYTT
                     SSFRSVEIFLRGAMGELLAMSILPLILLGFIKLYDSKKESWVMLAISMTLLIYTHVLS
                     VAMTMVMIIIALI"
BASE COUNT          174 a           85 c           82 g          251 t
ORIGIN      
        1 ttttttctgt aaaaatattt atagtttatg catttgttag tttattatat ttatttccag
       61 catttaaaaa catggaaata tttggctttt atgatacggc atttcacatt aatcgtgcgt
      121 taagtttaga atctattttt agctccccta ttaattttga aacgtttaga tcatatggca
      181 tgcaagtaaa taatttttac ccatggttaa ctctatatcc tttattttta ttgatcaagt
      241 ttactaattt agcaataggc tataaccttt ttctttatat tgttacgctc attactctat
      301 ttatatgtca ttatgtaatg tatgaaatta ccaaaaaaca tgttactagc tcctttttcg
      361 ctattatata tacaacatct tcttttagga gtgtagaaat atttcttcga ggggcaatgg
      421 gggaattact cgcaatgtca atacttcctc ttattttact cgggtttata aagctttacg
      481 actcaaaaaa agaatcttgg gttatgttgg cgattagcat gactttatta atttacactc
      541 atgttctttc tgtagctatg actatggtaa tgattataat tgctttaatt at
//
LOCUS       DACDPS010000275          589 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.289, whole genome shotgun sequence.
ACCESSION   DACDPS010000275 DACDPS010000000
VERSION     DACDPS010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.289"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>589
                     /locus_tag="IWJ07_002938"
     CDS             <1..>589
                     /locus_tag="IWJ07_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9334427.1"
                     /translation="DRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHP
                     WEDYRNFFVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTL
                     IIEHETGIVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQL
                     YDEVMHDNNYSHTIIEDAYLEEIKEFFTLLEKKMEP"
BASE COUNT          212 a           73 c          104 g          200 t
ORIGIN      
        1 gatagattac tttatctatc ttctacattt ttgaagagaa aagaaattca atatattaag
       61 tctaaagtga cgcataataa aaaaatttct tatcaatacc atgtaggaca gtatttaccc
      121 gactggcatc cttgggaaga ttatcgcaat ttttttgttt caaacaaaaa aactaatggg
      181 tgtagagaaa tattcgctat tgaattacct tggattatag atacatttgg gaaagttaaa
      241 tcgtttactg taatgaccca gaaattatcc aatcttgata ttgattattc ggacagtttc
      301 actcttatta ttgagcatga aactggtata gttggaactt taagtgtaga tgttgtttca
      361 agagtagcta aaagaaattt aactataatt ggtgaaaaga ttttaataga atgggaaggt
      421 aacccggatt ctctattttc atggaatgaa gaaacttcta gtatgacaga tattcaattg
      481 tacgatgagg ttatgcatga taataattat tcacatacta ttattgaaga tgcatattta
      541 gaggagatta aagaattttt cacgcttttg gaaaagaaaa tggaacccc
//
LOCUS       DACDPS010000276          572 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.290, whole genome shotgun sequence.
ACCESSION   DACDPS010000276 DACDPS010000000
VERSION     DACDPS010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.290"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
BASE COUNT          198 a           84 c           79 g          211 t
ORIGIN      
        1 ttattgcttt tgcaaaattt atttacctgt tttctggatc aacgatagtg tacattatgc
       61 tatccttgtt tggattttag gacgtttaaa gaggcacttt tttacataac ttaatccata
      121 gaccttttaa gcatgctgtg agataataat aaagaagagt ttaaagcgca ccccaaacca
      181 cttccccata agtgtattac gctttaaact cttttatatt tgaattcact aagaagcata
      241 ccatatattt gaatttacta agaagcatac catatttttg aaaaaaagtg agaaaaaagg
      301 cttataatta ggatttagag taattaatta gatgtttatt ttataggact gatactatag
      361 attacaaata gtatcgtatt tcgcaatctt aaaattatct tcttactgtg aataagataa
      421 taaaatttgt atttgtcctt tttaggtaac ttattttaat ctatatacct ttctggatca
      481 acgaaagtat actttatata gtcataacgc cgatggtaac tatgcactgt aacgataatg
      541 ctgctaaaat atctttttac gaaaaacatc ac
//
LOCUS       DACDPS010000277          565 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.291, whole genome shotgun sequence.
ACCESSION   DACDPS010000277 DACDPS010000000
VERSION     DACDPS010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.291"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            302..>565
                     /locus_tag="IWJ07_002939"
     CDS             302..>565
                     /locus_tag="IWJ07_002939"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP9334428.1"
                     /translation="MSAVVTDLTYVRVGTNWAYICLMIDLFNREIIGHSCGNKKDAQL
                     VKRAIQSIPYSLQEIELFHTDRGKEFDNQTIQNLMNGLVIKFLS"
BASE COUNT          193 a          102 c           82 g          188 t
ORIGIN      
        1 tctattattc ttttcataaa tattgtaaat agaagtatta aaaatatata caaaaagtat
       61 tatttcgaga aagataaata ttttcaaaaa ccttttcata atcctcaccc tttcttacac
      121 tgataaatca tgcgtctaat attcaaatta tactcgatat gctttagcta atcagccaac
      181 tctgtttgtc tactatcatt atatcaatag acaatatctg ctcccgtctt tatgatcgtg
      241 ccaaagcaat caaaagaaaa ttgcgaatca cttagatcga aagtttactc aaacaaaagc
      301 attgtcagcg gttgtcacgg atttaactta tgttcgggtc gggacaaatt gggcttatat
      361 ttgtttgatg attgatttat tcaatcgtga aattattggc cattcttgcg gaaataaaaa
      421 agatgcgcag cttgtaaaaa gggccattca aagcattcct tactcacttc aagaaatcga
      481 actcttccat acggatcgtg gaaaagaatt tgataaccaa accattcaga atctcatgaa
      541 tggtttggtt atcaaattcc tttcc
//
LOCUS       DACDPS010000278          543 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.292, whole genome shotgun sequence.
ACCESSION   DACDPS010000278 DACDPS010000000
VERSION     DACDPS010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.292"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..323
                     /locus_tag="IWJ07_002940"
     CDS             <1..323
                     /locus_tag="IWJ07_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9334429.1"
                     /translation="SIRKRNANIKDNEEPLADSNEGRYSQTHFDEQDVYFLFGMEILI
                     SDQKLSAAIDQLSEIRRKIILLYYFAGFNDAEIGKILNMSTSGIWYQRKKAVEQLKMG
                     YDLW"
     gene            317..>543
                     /locus_tag="IWJ07_002941"
     CDS             317..>543
                     /locus_tag="IWJ07_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9334430.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLLEAI"
BASE COUNT          196 a           77 c          103 g          167 t
ORIGIN      
        1 agtctattcg aaaacgaaac gctaacataa aggataatga ggaaccattg gcagattcaa
       61 atgaaggcag gtattcccaa actcattttg atgaacaaga tgtctatttc ttatttggaa
      121 tggaaatatt aatttcagat caaaagttat cagcagcaat tgatcagtta tcggagataa
      181 gaagaaagat tatcctactt tattattttg caggttttaa tgatgctgaa atcggtaaaa
      241 ttctcaatat gagcacaagt ggtatctggt atcaacgaaa gaaggcagtt gaacaattga
      301 aaatggggta tgacctatgg tagataaatc taaaaagcgg cagtacctat tagttccact
      361 atccattatc ttatctgctc tagaaggaga tccaatttcg atcaagatga tactcaaaca
      421 ttatcattct tatatggcta agttatgttt gacaaatgga tttaacgagg taggtcaatt
      481 cgttacctat gtagatgaat atatgataag tcagctagaa attaaattat tagaagcgat
      541 ctt
//
LOCUS       DACDPS010000279          532 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.293, whole genome shotgun sequence.
ACCESSION   DACDPS010000279 DACDPS010000000
VERSION     DACDPS010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.293"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(151..441)
                     /locus_tag="IWJ07_002942"
     CDS             complement(151..441)
                     /locus_tag="IWJ07_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP9334431.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
BASE COUNT          175 a           79 c           80 g          198 t
ORIGIN      
        1 tgcgagcagc aactagaata atttaaacat tattaaactg tctatattgt tactaataat
       61 tcccttctta tagtgcattt tcttagaaaa aaacaatatt acgatatcat gatattaaga
      121 tatcgtaata ttatacaatt gtgattgata ttatatatgt ttttttattt ttttatcatg
      181 tttaatcttg aaggaatcat attctttttc taaagacagg agcttttcat aaacatcaga
      241 catgtttaaa ctagaggcca ggcttaaatc ataaacgaat gaagcaggta atgattttat
      301 tctacgttgt cgggttatcc agcttgcaac cgttccttgt gggtaggcat ataactcaca
      361 aaattgttct acagtgattc ctaaattctt gataatgtaa acattgatcg ggtgcgagta
      421 aacaaatgta ttgatagaca tggtgcgtaa gcctactttc ttttattatt gcgtttgcaa
      481 taatagttta acacacgcat tattctagta tctactccta tttgtaaaaa ca
//
LOCUS       DACDPS010000280          516 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.294, whole genome shotgun sequence.
ACCESSION   DACDPS010000280 DACDPS010000000
VERSION     DACDPS010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.294"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(216..>516)
                     /locus_tag="IWJ07_002943"
     CDS             complement(216..>516)
                     /locus_tag="IWJ07_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HAP9334432.1"
                     /translation="EAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEV
                     QKGMTIHSIRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
BASE COUNT          163 a           99 c           89 g          165 t
ORIGIN      
        1 gagaaaatgt agcctgaata attcatagct ttaattcttt ggcacatttt attgaaatat
       61 gtatcactaa tatccctatc aactgatttt gactaaaaag ttacttcaaa cattcactgt
      121 tcaaaaatat tgaggaataa attttgggtg taaggcattt tgagcatact tctccctgga
      181 taacttccca gtaaaaaaat cgttagattg ttggattaga tccattgact ggctcgcctc
      241 aggcgagcaa agaccctgta gaattcattc tgatacacat gataactaga taatttgaga
      301 tagactcgtc tacccgtttg gactaatttc ccagcaactt tcagaaactt caatcgaata
      361 gaatgaatgg tcattccctt ttggacttct tcaaagccaa tcgttcttaa gaagttgact
      421 aagttgtaag ctatcaagct gagcatcatt cggacatgat tctccaaaaa gcgaggactg
      481 tctgttttgt caaagtaaaa gccagctttc gcttct
//
LOCUS       DACDPS010000281          512 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.295, whole genome shotgun sequence.
ACCESSION   DACDPS010000281 DACDPS010000000
VERSION     DACDPS010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.295"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            94..>512
                     /locus_tag="IWJ07_002944"
     CDS             94..>512
                     /locus_tag="IWJ07_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9334433.1"
                     /translation="MHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQ
                     GYVSWGAGNANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTS
                     LFTKGIISHLWVTNYVWGNHTDPYGYLSQMGVSKEKLA"
BASE COUNT          175 a           84 c          107 g          146 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gc
//
LOCUS       DACDPS010000282          510 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.296, whole genome shotgun sequence.
ACCESSION   DACDPS010000282 DACDPS010000000
VERSION     DACDPS010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.296"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
BASE COUNT          195 a           64 c           71 g          180 t
ORIGIN      
        1 actaccttta ataggtagta ctttattatc aagccattat tccttcttct actagttctt
       61 tttgtcacat aattttgcaa tcaataaatg ttgcgcaata agtataagaa ttccattttc
      121 taaatttgaa aggtgatcca taatttcatg ttcaagtagt ttattaattg tccacttgtc
      181 gcttcatcta aaaattttat aggttttagg aatgctattc gcttatttta ggaagatttt
      241 aactgtttgc tatagatata tatcatacta gtatcgtaat aaaagtactc taaaagaaga
      301 attttcaaat acgcaaaatt aagtacgaaa gagttgaaac aaatttggag aaaatgagca
      361 tagaatgtct gatgagttaa atagacctaa aaattatcta aaagtggagc aaaattagga
      421 attacaaaca aaacactaaa ttagtttata taaatttccg taatatttaa tatatatgaa
      481 aaggaaaacg tagaaaatta cgtctacgtt
//
LOCUS       DACDPS010000283          496 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.298, whole genome shotgun sequence.
ACCESSION   DACDPS010000283 DACDPS010000000
VERSION     DACDPS010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.298"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..387
                     /locus_tag="IWJ07_002945"
     CDS             <1..387
                     /locus_tag="IWJ07_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP9334434.1"
                     /translation="RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGK
                     CAITLEEFQQANEIHCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIAL
                     LKLNKVQLIRLNKYRVLAGNQELNLI"
BASE COUNT          193 a           78 c           89 g          136 t
ORIGIN      
        1 agaaaattta tccacaccaa tctaagtgtt gataaattac tgatgtacca gttaatgaaa
       61 ttatctttgg aaaatcgcac aatagaatac gcagacaatc gaatttcgct attttctgct
      121 caacatggaa aatgtgctat aactctggaa gaatttcaac aagctaatga aatacattgt
      181 catcatattg taccaactgg agctggtgga aatgacgatt ataaaaatct tattttggta
      241 aaagaggcgg tacaccgtct aatacacgct aaaacagaag aaacaataca aaaatatata
      301 gctctattga agctaaataa agtccagctc ataagactaa ataaatatag agtattagct
      361 ggcaatcaag aattaaactt gatataggtc tactacataa atacttttca atataaacac
      421 gatggaacgc ggagtgcggt gaaagtcgca cgctccgtgt gaatgggggg aaaagtttga
      481 gatatttata atatca
//
LOCUS       DACDPS010000284          457 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.301, whole genome shotgun sequence.
ACCESSION   DACDPS010000284 DACDPS010000000
VERSION     DACDPS010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.301"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
BASE COUNT          141 a           73 c          106 g          137 t
ORIGIN      
        1 ggagcgatcc gaccacgttg gcgggaaaat ccttaagatt tttccgccat gcttgcaagg
       61 gggcggttcg cttcggcggt tcggcgagct tgcgagccgt tcaaagcccc cgttatctaa
      121 caaggggggc acaaaaaaca agaaacacta tacactaaca agcggaaaac gctgtattta
      181 taaggcaaaa ccccattttt acagtagtga tataaattgt acctatatca ctatgaaaat
      241 tgaatagtaa atatgagaaa gacagttgat ttcaaaaaat aagaaattga ttgtcgtttt
      301 cttttgcaga aatttaagtg caggcaatta tggtagttgt cgtaattgct gtcagctcag
      361 tgtttatcac tgatggtgtg aaagatttac ggttggagat gttcaataag attattggtg
      421 cttaattatt tttattgtag tgctgatatg agaatgt
//
LOCUS       DACDPS010000285          449 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.302, whole genome shotgun sequence.
ACCESSION   DACDPS010000285 DACDPS010000000
VERSION     DACDPS010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.302"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>449
                     /locus_tag="IWJ07_002946"
     rRNA            <1..>449
                     /locus_tag="IWJ07_002946"
                     /product="23S ribosomal RNA"
BASE COUNT          136 a           99 c          132 g           82 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggca
//
LOCUS       DACDPS010000286          440 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.304, whole genome shotgun sequence.
ACCESSION   DACDPS010000286 DACDPS010000000
VERSION     DACDPS010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.304"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            11..>440
                     /locus_tag="IWJ07_002947"
     CDS             11..>440
                     /locus_tag="IWJ07_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9334435.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          168 a           57 c           88 g          127 t
ORIGIN      
        1 ggtgcttttt ttgcggaaat atacatttgt ttttaagaaa aaagtagttt cagactattt
       61 aaataacgaa ggcggctaca aatatcttgc acataaatat caaataaatc gtacactggt
      121 tagacattgg gtaaggattt ataactatca tggttgggaa ggcttggttg gaggtggcaa
      181 aagctacact acaaaattta aacttgatgt tatagaatat atggaaacaa atggtctttc
      241 tatccaagaa actgctaaaa aatttaatat cggttcaaat agaactctaa gtaaatggat
      301 agagcaatat gaagaaggcg gtgcttcttc acttgagagc caaaaaaggg gcagaaaaat
      361 tagtatgaat tccaagctaa acattcctaa aaaacttaaa gatgagtctc ttgaagaaga
      421 agttattcgt ttaagagcag
//
LOCUS       DACDPS010000287          424 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.306, whole genome shotgun sequence.
ACCESSION   DACDPS010000287 DACDPS010000000
VERSION     DACDPS010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.306"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..375
                     /locus_tag="IWJ07_002948"
     CDS             <1..375
                     /locus_tag="IWJ07_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334436.1"
                     /translation="EWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIVDYFANTP
                     EEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKKKEYNYIV
                     KAKVSKRFIFNPKLEIYSVVIE"
BASE COUNT          182 a           42 c           70 g          130 t
ORIGIN      
        1 gaatggaata atctgactca aaataataaa aataattctg gatgtggaat tatctttatg
       61 tttgttatag ttttgggaat agtagtactt ttaataactg tcaagatagt agattacttt
      121 gcgaatactc ctgaagaaca aatacagaga aaacttcctg agtttgaaag agtaccacct
      181 tatgatccaa gagataattc atttcaagta aaaaaattaa aagagtctcc aaatgaaatg
      241 tggtttgaag ttacagttga atcaagcgac ttcaaagaaa aaaagaaaga atacaattat
      301 attgtaaagg caaaagtatc aaaaagattt atttttaacc ctaaattaga aatatatagt
      361 gttgtaatag aataatagtg ttaaaaaaat tgtaaaagtt aaagaagtca aattctgatg
      421 tgaa
//
LOCUS       DACDPS010000288          421 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.307, whole genome shotgun sequence.
ACCESSION   DACDPS010000288 DACDPS010000000
VERSION     DACDPS010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.307"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>421
                     /locus_tag="IWJ07_002949"
     CDS             <1..>421
                     /locus_tag="IWJ07_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9334437.1"
                     /translation="DRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFD
                     PTRKAVIKGKDSLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFA
                     ELLGLTWEDIDFEDQIITINKTWNYKLNEWGKTKNETS"
BASE COUNT          163 a           61 c           77 g          120 t
ORIGIN      
        1 gatagagtgg gatatcaacg ctttttaaat aagtatgcta aagaccatgt aaaatctacc
       61 gttattaagt ttaataacca tattagagcg tcgttgaaag atgccgtaga agaaggatta
      121 attccgtttg atccaactag aaaagcagta atcaaaggaa aagattcatt gaagccaaaa
      181 gaagataaat atttagatta tgatcaattt aaatctttaa tgaaactcgt agaagaaaac
      241 ctttctgcgc agtactcttc tcctatgctc gtgttagttg ctggtgccac tggaatgcga
      301 tttgctgaac ttctaggatt aacatgggaa gatatcgatt tcgaagatca aatcatcact
      361 attaataaaa catggaatta taaattaaat gaatggggaa aaacaaaaaa cgaaacttca
      421 a
//
LOCUS       DACDPS010000289          420 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.308, whole genome shotgun sequence.
ACCESSION   DACDPS010000289 DACDPS010000000
VERSION     DACDPS010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.308"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            6..>420
                     /locus_tag="IWJ07_002950"
     CDS             6..>420
                     /locus_tag="IWJ07_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HAP9334438.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGETKKLLKNINLANINYSDELPLSPL"
BASE COUNT          147 a           54 c           75 g          144 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaaact aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgatga acttccttta tcccctttac
//
LOCUS       DACDPS010000290          415 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.309, whole genome shotgun sequence.
ACCESSION   DACDPS010000290 DACDPS010000000
VERSION     DACDPS010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.309"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..339)
                     /locus_tag="IWJ07_002951"
     CDS             complement(<1..339)
                     /locus_tag="IWJ07_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9334439.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DACDPS010000291          390 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.312, whole genome shotgun sequence.
ACCESSION   DACDPS010000291 DACDPS010000000
VERSION     DACDPS010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.312"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>390
                     /locus_tag="IWJ07_002952"
     CDS             <1..>390
                     /locus_tag="IWJ07_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HAP9334440.1"
                     /translation="SRFTRLSSNLAYTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKP
                     VRNRQAKLLNQIAKVGYNSTKKSYFYGLKIHMIVTKTGFPITYSITNPGVHDVKVLET
                     LSEEANLPNILGDKGYISHKIHEKLAL"
BASE COUNT          138 a           74 c           63 g          115 t
ORIGIN      
        1 gagtcgattc actcgcttaa gttcaaactt agcttacact atcaagatta ttcgatactt
       61 tttcatcaag aagctcacca aaggtgaact agtcggaatt atagatagtt ttcctagtcc
      121 attatgtaaa ccagttagaa atagacaagc aaaactattg aatcaaatag ccaaagttgg
      181 ctataactca acaaaaaaat cttattttta tggtcttaaa attcatatga tcgttactaa
      241 gactggcttt ccgattactt actcaatcac gaatccaggt gtccatgacg tgaaagtgtt
      301 agagacttta tcggaagaag cgaatcttcc caatatccta ggggacaaag gatatattag
      361 ccataaaatc catgaaaagc ttgcccttaa
//
LOCUS       DACDPS010000292          368 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.314, whole genome shotgun sequence.
ACCESSION   DACDPS010000292 DACDPS010000000
VERSION     DACDPS010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.314"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>368
                     /locus_tag="IWJ07_002953"
     CDS             <1..>368
                     /locus_tag="IWJ07_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HAP9334441.1"
                     /translation="LSQEEKIKNIAGIYAAKRPIKDLTEKYDLIINIANVNPGTVQRI
                     VWPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKL
                     MGLSEFKGISPIDAYCGLKDT"
BASE COUNT          138 a           51 c           64 g          115 t
ORIGIN      
        1 ttatcgcaag aagaaaaaat taaaaatata gctggaatat atgctgctaa gagaccaatc
       61 aaagatttaa ctgaaaaata tgacttgatt attaatatag ctaatgttaa cccaggtaca
      121 gttcaaagaa ttgtttggcc agcaagtaaa ggtactccgg atattccatt ttatattcat
      181 gaaattccaa caatttttgt atctgtacag tgtccgtttc atttagctga tgtgcctcaa
      241 gtaaaaactt atatcaatac ttatgatgga aaacaagaaa ctttagaaat attagtggac
      301 aaactaatgg gcctttctga atttaaggga ataagtccaa tagatgctta ttgtgggtta
      361 aaggatac
//
LOCUS       DACDPS010000293          364 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.315, whole genome shotgun sequence.
ACCESSION   DACDPS010000293 DACDPS010000000
VERSION     DACDPS010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.315"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>364
                     /locus_tag="IWJ07_002954"
     CDS             <1..>364
                     /locus_tag="IWJ07_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HAP9334442.1"
                     /translation="EDIFAYILEQAVKAGFVTEDNLYLDSTHIKANANKHKFTKEMTH
                     DEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPESGYYVKGEREK
                     QFAYSAHTSCDDNGFILST"
BASE COUNT          137 a           62 c           76 g           89 t
ORIGIN      
        1 tgaggatatt tttgcctata ttttagaaca agcagttaaa gcaggttttg taacagagga
       61 taatttgtat ctggattcta cccacatcaa agctaacgcc aataaacata agtttacgaa
      121 agaaatgacc catgatgaag cgaaagccta tcaggatgaa ttagaagatg agatcaatcg
      181 tcaacgaata gaggctggaa aacgcccttt tactttggac ttagaaaagg aagtgaagct
      241 gaaagaaaga aaaataagta aagctgatcc ggaaagcggc tactacgtca aaggcgaacg
      301 agaaaaacaa tttgcttatt ctgcccatac ttcatgcgac gacaatggat ttattcttag
      361 caca
//
LOCUS       DACDPS010000294          356 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.316, whole genome shotgun sequence.
ACCESSION   DACDPS010000294 DACDPS010000000
VERSION     DACDPS010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.316"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>356
                     /locus_tag="IWJ07_002955"
     CDS             <1..>356
                     /locus_tag="IWJ07_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1D family transposase"
                     /protein_id="HAP9334443.1"
                     /translation="VQEYSKVLYYLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAID
                     ADGLTLDIWLRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYT
                     KTEHRTVKYLNNLIEQ"
BASE COUNT          122 a           66 c           74 g           94 t
ORIGIN      
        1 gtgcaagagt acagcaaagt cctctattat ctttggaaga agaaaaatag acaatccttc
       61 tattcatgga aaatggacga aacctatatc aaaattaagg gacgttggca ttatctttat
      121 cgtgcaattg atgcggacgg cttaacctta gatatctggt tacgaaagaa acgggatacg
      181 caagcagcct atgctttctt aaaacgactc cataaacagt ttggtgagcc gaaagcaatt
      241 gtgaccgata aagcaccttc tcttggctcc gcctttagaa agttacagag tgtgggttta
      301 tatactaaga cagagcaccg aactgtgaag tatcttaaca atttaataga acaaga
//
LOCUS       DACDPS010000295          352 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.denovo.319, whole genome shotgun sequence.
ACCESSION   DACDPS010000295 DACDPS010000000
VERSION     DACDPS010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.denovo.319"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(120..332)
                     /locus_tag="IWJ07_002956"
     CDS             complement(120..332)
                     /locus_tag="IWJ07_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334444.1"
                     /translation="MIFQGVITLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIIL
                     SFLVNAIVLFLYVLNYLDINPLIFSR"
BASE COUNT          147 a           54 c           60 g           91 t
ORIGIN      
        1 attgcataca tcacaaatga aatgctagga aaataaaaga agaatagatc aatgaaaaat
       61 gaaaaagcag aacagttttc tgctgataag caggaactgt tccgctgaat gtgtcttatc
      121 tatcgagaaa aaatcaatgg attgatatct aagtaattca atacatataa aaagaggacg
      181 atcgcattca ctaaaaagga aagaatgatg atcccatagc gaatggaaac atatttaaca
      241 aatacttttg tttgaatacg ggcatatctt tttcctttat agctgagcat catgacaaga
      301 agcaaaaggg taatcacacc ttgaaaaatc attccaataa acagaattcc tg
//
LOCUS       DACDPS010000296         4170 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.guided.136, whole genome shotgun sequence.
ACCESSION   DACDPS010000296 DACDPS010000000
VERSION     DACDPS010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4170
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.guided.136"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(160..531)
                     /locus_tag="IWJ07_002957"
     CDS             complement(160..531)
                     /locus_tag="IWJ07_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9334445.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            676..861
                     /locus_tag="IWJ07_002958"
     CDS             676..861
                     /locus_tag="IWJ07_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP9334446.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            1373..1591
                     /locus_tag="IWJ07_002959"
     CDS             1373..1591
                     /locus_tag="IWJ07_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334447.1"
                     /translation="MRQERPEGVHTGPACVKSHTARCIRKKEAAHFAEAWGVPNKQNE
                     NAAGGQVVDQNIRKGYSDPLLASIIGMF"
     gene            1804..2463
                     /locus_tag="IWJ07_002960"
     CDS             1804..2463
                     /locus_tag="IWJ07_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HAP9334448.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            2466..3809
                     /gene="vanS"
                     /locus_tag="IWJ07_002961"
     CDS             2466..3809
                     /gene="vanS"
                     /locus_tag="IWJ07_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP9334449.1"
                     /translation="MERKGIFIKVFSYTIIVLLLVVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQNKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLGNIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            3980..>4170
                     /locus_tag="IWJ07_002962"
     CDS             3980..>4170
                     /locus_tag="IWJ07_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-B/VanY-G family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAP9334450.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLRWR
                     LASVLEATQIPPIPATHTG"
BASE COUNT         1202 a          872 c         1016 g         1080 t
ORIGIN      
        1 gctacactcc ctatgcttgc tacgttactg tttctccact aacaagacgt ttccaaagcc
       61 ccaaactacc cgcaaaatag ccctctggaa agaaaaaagt gcaaaaaaat agaagctacg
      121 ctgtgcagct cccgctaaaa gtaaaacaga ggatgccagt tacacatcct ccgtttcatt
      181 cttcttcggc ttttcaatta gtatggcgct gtcaatcgcg ccctcgatga tcggcaggta
      241 ttcttcgggg cagagtttca gcttgtggct gaccctttgc cgcagttcgc tttcctcccg
      301 catgatctcc gggttaaagt agcgttccac aggaagcccg caggctttaa taagctgtat
      361 gacaaccggc agactgggaa tggccccgtc gttttcgatg ttggcaagat agcgccagtc
      421 aatatttatt ttctctgcca ttgctttgcg cgaaacgcgc tttgcttgcc gggccgcttt
      481 cacatccgcg ccgaaagttt caaagccggg acaatcttca atattcgcca tatcgcacct
      541 cccacttaca ttgtatattt catactctgc ccgtggaatg ttggtatatg cacctttctt
      601 aaagtttata tttcacattt tgtttctttt attcttcgag caaaagaatt ataatgtacc
      661 ctgtggaggt gtactatgga ctacatgacg ttgaaagagg caggcgagaa atggggtgtg
      721 cccccacgat ggataaacta ctattgtgct gcgggacgca tccccggcgc tgtaaagatg
      781 gcgacgatct ggctcattcc caaaactgcg gaaaagccga ttgacaggcg tacaaagaaa
      841 ggaaaggcgg ttactgaata gtataatgaa accggcgcat tttggatatg gttttgaaga
      901 gagagaaagc atggtatttt gtcacggaaa aactataaga tgatagatgg caggctgctc
      961 cagaccgata aaaagtattc tcatctgaaa cagaaagaaa agatttacac atggatgtat
     1021 gaagaagcga aactttatca tgacagaaat ggaaaatgcc cgaaacaaag acgaggaagc
     1081 tgctgctgta tcagccgtat atggtagaat tgaaaaggcc ggcatctgga ttctttatgg
     1141 tgaagtctcc agacattgga taactgctgg tctaatgcgc ctttgagtat ggggtgattg
     1201 attttaatac cggggaggta cggctttttg aggaaaatat tctgggattt acatttggcg
     1261 attattacat ttcctgtaac tgcaaggaca cggatgatga agatatgagg aatatagact
     1321 tgctgatttt ctactgtctt gaacggtatt aggactgaat ctgctgcgcg gagtgcggca
     1381 agagcgaccg gagggagtac acacaggccc tgcctgtgtg aaaagccata ccgcaaggtg
     1441 cattcgcaaa aaagaggccg cgcattttgc tgaggcttgg ggtgtcccca acaagcaaaa
     1501 cgagaacgcc gcaggcgggc aagtggtgga tcagaatatc cgtaaaggat attctgatcc
     1561 attgcttgcc agtattatcg ggatgttttg acaaccgctg taatcgaaac atcccaaaca
     1621 ttaagaaggt gttaattatt attatcttga tttaattcgt ttaaaccaat aatatagata
     1681 catacaccat gaaaagaagc gagtatccca atgacggatg attaaaatcc gaaaagaaat
     1741 cgctttgaaa ctacagggaa actacagact gttatgttat cttcttaaaa tggagggatt
     1801 tttatgtcga tacgaattct acttgtcgag gatgatgatc atatctgtaa tacagtaagg
     1861 gcgtttctgg ctgaggcagg atatcaggtg gatgcctgca cagatggaaa tgaggcatac
     1921 accaagtttt acgaaaacac ttatcaactg gttattcttg atattatgct gcccggtatg
     1981 aacgggcatg aacttttgcg tgaatttcgt gcgaaaaatg atactcccat tctgatgatg
     2041 acagccctgt cggatgacga aaaccaaatc cgggcgtttg atgcagaggc agacgactat
     2101 gtaacaaagc cattcaagat gcagatttta ctaaagcggg tggaagccct gctgcggcgc
     2161 agcggtgcgc tggcaaagga aatccgtgtc ggcaggctga cacttctgcc ggaggatttc
     2221 acggtacttt gtgacggtac agagctgccc ctgacacgaa aagaatttga aatccttttg
     2281 ctgctggtgc agaacaaagg cagaacctta acgcatgaaa tcattttgtc acgtatatgg
     2341 ggatatgact ttgagggtga tggcagcaca gtccacactc atatcaaaaa tttgcgggcc
     2401 aagctgccgg aaaatatcat caaaaccatt cgcggtgtag gataccgatt ggaggaatca
     2461 taataatgga aagaaaaggg attttcatta aggttttttc ctatacgatc attgtcctgc
     2521 tactggttgt cggtgtaaca gcaacactgt ttgcacagca atttgtgtct tatttcagag
     2581 tgatggaatt acagcaaaca gtaaaatcct atcagccatt ggtggaactg attcagaata
     2641 gcgataggct tgatattcaa gaggtggcag ggctgtttca ctacaataac caatcctttg
     2701 agttttatat tgaagataaa gagggaagcg tactctatgc cacaccaaat gccaatacat
     2761 caaatagttt tagacccgac tttctttatg tggtacatag agatgataat atttcgattg
     2821 ttgctcaaaa caaggcaggt gtgggattgc tttatcaagg gctgacaatt cggggaattg
     2881 ttatgattgc gataatggtt gtattcagcc ttttatgcgc gtatatcttt gcgcggcaaa
     2941 tgacaacgcc gatcaaagcc ttagcggaca gtgcgaataa aatggcaaac ctgaaagatg
     3001 taccgccgcc gctggagcga aaggatgagc ttggcgcact ggctcatgat atgcattcca
     3061 tgtatgtcag gctgaaagaa accatcgcaa ggctggagga tgaaatcgca agggaacatg
     3121 agttggagga aacacagcga tatttctttg cggcagcttc tcatgagcta aaaacgccca
     3181 tcgcggctac aagcgttctg ttggagggaa tgcttggaaa tatcggtgac tacaaagatc
     3241 attctaagta tctgcgcgaa tgcatcaaaa tgatggatag gcagggcaaa atcatttccg
     3301 aaatactgga gcttgtcagc ctgaatgatg ggagaatcgt acccatagct gaaccgttgg
     3361 acatagggcg cacggttgcc gagttgctgc ccgattttca aaccttggca gaggcaaaca
     3421 accagcggtt cgtcacagat attccagccg ggcaaattgt cctgtccgat ccgaggctgc
     3481 tccaaaaggc actatccaat gtcatattga atgcagttca gaacacgccg cagggaggcg
     3541 aggtacggat atggagtgag cctggtgctg aaaaatgccg cctttttgtt ttgaacatgg
     3601 gcgttcacat tgatgatact gcgcttccaa ggctgttcac cccattctat cgcattgatc
     3661 aggcgcgaag cagaaaaagt gggcgaagcg gtttaggact tgccatcgta caaaaaacgc
     3721 tggatgccat gagcctccag tatgcgctgg aaaacacctc ggatggcgtt ttgttctggc
     3781 tggatttacc gctcacatca acattgtaaa tatttaaaac ttaaatgatt ttgaccgaca
     3841 ggtataaccc tgccggtctt tttttgctgc tacaggaaaa ctacagactg gctacaggga
     3901 aagtacagat gcgcttgcca taataacaat cgtaccagcc acaaatcgta gttttattgc
     3961 aaaggaggca ttcgatcaaa tggaaaaaag caactatcat tccaatgcgg atcatcacaa
     4021 acggcatatg aaacaatccg tggaaaagcg ggcttttcta tgtgcgttca ttatttcgtt
     4081 cacagtctgc acgctgtttt tgaggtggag actggcttcc gtattggagg caacacagat
     4141 accgcccatc cctgcaactc atacaggcag
//
LOCUS       DACDPS010000297         3965 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.guided.141, whole genome shotgun sequence.
ACCESSION   DACDPS010000297 DACDPS010000000
VERSION     DACDPS010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3965
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.guided.141"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..933
                     /locus_tag="IWJ07_002963"
     CDS             <1..933
                     /locus_tag="IWJ07_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9334451.1"
                     /translation="IVMIAIMVVFSLLCAYIFARQMTTPIKALADSANKMANLKDVPP
                     PLERKDELGALAHDMHSMYVRLKETIARLEDEIAREHELEETQRYFFAAASHELKTPI
                     AATSVLLEGMLGNIGDYKDHSKYLRECIKMMDRQGKIISEILELVSLNDGRIVPIAEP
                     LDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLSDPRLLQKALSNVILNAVQNTP
                     QGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFTPFYRIDQARSRKSGRSGLGLA
                     IVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            1104..1910
                     /gene="vanY"
                     /locus_tag="IWJ07_002964"
     CDS             1104..1910
                     /gene="vanY"
                     /locus_tag="IWJ07_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-B/VanY-G family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAP9334452.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLRWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            1928..2755
                     /gene="vanW"
                     /locus_tag="IWJ07_002965"
     CDS             1928..2755
                     /gene="vanW"
                     /locus_tag="IWJ07_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HAP9334453.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATIPEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            2752..3723
                     /locus_tag="IWJ07_002966"
     CDS             2752..3723
                     /locus_tag="IWJ07_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HAP9334454.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTARGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            3716..>3965
                     /locus_tag="IWJ07_002967"
     CDS             3716..>3965
                     /locus_tag="IWJ07_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HAP9334455.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKES"
BASE COUNT         1144 a          851 c         1044 g          926 t
ORIGIN      
        1 attgttatga ttgcgataat ggttgtattc agccttttat gcgcgtatat ctttgcgcgg
       61 caaatgacaa cgccgatcaa agccttagcg gacagtgcga ataaaatggc aaacctgaaa
      121 gatgtaccgc cgccgctgga gcgaaaggat gagcttggcg cactggctca tgatatgcat
      181 tccatgtatg tcaggctgaa agaaaccatc gcaaggctgg aggatgaaat cgcaagggaa
      241 catgagttgg aggaaacaca gcgatatttc tttgcggcag cttctcatga gctaaaaacg
      301 cccatcgcgg ctacaagcgt tctgttggag ggaatgcttg gaaatatcgg tgactacaaa
      361 gatcattcta agtatctgcg cgaatgcatc aaaatgatgg ataggcaggg caaaatcatt
      421 tccgaaatac tggagcttgt cagcctgaat gatgggagaa tcgtacccat agctgaaccg
      481 ttggacatag ggcgcacggt tgccgagttg ctgcccgatt ttcaaacctt ggcagaggca
      541 aacaaccagc ggttcgtcac agatattcca gccgggcaaa ttgtcctgtc cgatccgagg
      601 ctgctccaaa aggcactatc caatgtcata ttgaatgcag ttcagaacac gccgcaggga
      661 ggcgaggtac ggatatggag tgagcctggt gctgaaaaat gccgcctttt tgttttgaac
      721 atgggcgttc acattgatga tactgcgctt ccaaggctgt tcaccccatt ctatcgcatt
      781 gatcaggcgc gaagcagaaa aagtgggcga agcggtttag gacttgccat cgtacaaaaa
      841 acgctggatg ccatgagcct ccagtatgcg ctggaaaaca cctcggatgg cgttttgttc
      901 tggctggatt taccgctcac atcaacattg taaatattta aaacttaaat gattttgacc
      961 gacaggtata accctgccgg tctttttttg ctgctacagg aaaactacag actggctaca
     1021 gggaaagtac agatgcgctt gccataataa caatcgtacc agccacaaat cgtagtttta
     1081 ttgcaaagga ggcattcgat caaatggaaa aaagcaacta tcattccaat gcggatcatc
     1141 acaaacggca tatgaaacaa tccgtggaaa agcgggcttt tctatgtgcg ttcattattt
     1201 cgttcacagt ctgcacgctg tttttgaggt ggagactggc ttccgtattg gaggcaacac
     1261 agataccgcc catccctgca actcatacag gcagcagcac tgacgtagtg gagaatttgg
     1321 aggaaaacgc tcttgccacc gccaaagaac agggagatga acaggaatgg agcctgattt
     1381 tagtgaacag gcagaacccc atccccgcac agtacgatgt ggagcttgag caactatcaa
     1441 atggtgagcg gatagatatt cggatttctc cctatcttca agatttgttt gatgccgcaa
     1501 gaactgatgg agtttacccg attgtcgcat ccggataccg aacaacagaa aaacagcaag
     1561 aaattatgga tgaaaaaatt gccgaatata aggcgaaagg ctacacctct gcacaggcta
     1621 aagcggaagc agaaacttgg gtggccgtgc cgggaacgag cgagcatcag cttggtcttg
     1681 ctgtggatat caatgcggac ggaattcatt caacaggcaa cgaggtttat agatggctgg
     1741 atgaaaacag ctatcgtttt ggttttattc gtcgctaccc accagacaag acagagataa
     1801 ccggtgtgag caacgagccg tggcattacc gatatgttgg catcgaagct gccacagaga
     1861 tgtacaacca aggggtttgc cttgaggaat atttaaaacc agaaaaatga gaaaaggatg
     1921 ttatgttatg gacagaaaaa gattgacaca gcgtttcccg ttcctgcttc caatgagacg
     1981 ggcgcagaga aaaatgtgct tttatgcggg aatgagattt gacggctgtc gctatgcgca
     2041 gacgataggg gaaaaatcgc tttcccattt gctctttgaa acggattgcg cattatacaa
     2101 ccacaatacc ggatttgaca tgatatacca ggaaaacaag gtgttcaact taaagctggc
     2161 agcaaagacc ttaaacggct tattgataag accgggggaa accttttctt tctggtggct
     2221 ggtacgccat gcggacaaag atacccccta taaagacggc cttacggtga ccaatggtaa
     2281 actcaccacc atgtcgggcg gcggtatgtg ccagatgagc aatttactat tttggatgtt
     2341 cctgcatacg ccattgacaa ttatccagcg cagaggtcac gaagtaaagg agtttccaga
     2401 gccaaacagt gacgagatta aaggggtgga tgcaaccatt ccagagggct ggattgattt
     2461 aaaagtgcga aacgataccg actgcaccta ccaaatatgg gtgaccttag atgatgagaa
     2521 aatcatcggt caggtgtccg ccgacaaaga gccccaagca ttatacaaaa ttacaaatgg
     2581 cagtatccag tatgtccgtg aaagtggcgg gatttatgaa tatgcccagg ttaaacggat
     2641 gcaagttgcc ttaggtaccg gggaaataat agattgcaag ctgctttata caaacaaatg
     2701 caaaatctgt tatcccctcc cggaaagtgt ggatattcag gaggagaacc aatgagaaaa
     2761 agtgtgggca ttactgtttt tggatgcgaa caggatgagg caaatgcttt ccgcgcttta
     2821 tcgccggatt ttcatattat ccctacgctg attagcgatg cgatatcggc agacaacgca
     2881 aaattggccg ctggcaatca atgcgttagc gtaggccata agtccgaggt ttccgaggcg
     2941 acaattcttg cgctgagaaa ggtcggggta aaatacattt ctacccgcag catcggctgc
     3001 gatcacattg atacgactgc cgccgagaga atgggaatct cggttggcac ggttgcgtat
     3061 tcgccggaca gcgttgcgga ttatgctttg atgctgatgc tgatggccat acggggtgca
     3121 aaacccacca tgcacgccgt ggcgcaacaa gatttcagat tggatcgtat ccgggggaaa
     3181 gaactggggg atatgactgt gggagttatt ggaaccggcc atatcgggca agcggtcgtc
     3241 aaaaggctgc ggggatttgg atgccatgtg ctggcctatg ataacagccg aaaaatggat
     3301 gcagattatg tccagcttga tgagcttcta aaaaacagcg atattgttac gctccatgtg
     3361 ccgctttgtg cggatacccg ccatctgatc ggtcagaagc aaattggaga gatgaagcaa
     3421 ggcgcatttt taatcaacac cgcgcgcgga gcgcttgtcg ataccggggc gctggtggag
     3481 gcgttggaaa gcggaaagct gggtggtgcg gcactggatg tgttggaggg cgaggatcag
     3541 tttgtttata ccgattgctc gcagaaagtg cttgaccatc cctttttgtc gcagctttta
     3601 aggatgccga atgtgatcat cacaccccat acggcgtact acactgagcg tgtgctgcag
     3661 gatactacag aaaaaacaat caggaattgt ctcaattttg aaaggagttt acagcatgaa
     3721 tagaataaaa gtcgcaatca tcttcggcgg ttgctcggag gaacatgatg tgtcggtaaa
     3781 atccgcaata gaaattgctg cgaacattga tacggaaaaa ttcgatccgc actacatcgg
     3841 aattacaaaa aacggtgtat ggaagctatg caagaagcca tgtacggaat gggaagccga
     3901 cagtctcccc gccatactct ccccggatag gaaaacgcat gggctgcttg tcatgaaaga
     3961 aagcg
//
LOCUS       DACDPS010000298         3480 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.guided.150, whole genome shotgun sequence.
ACCESSION   DACDPS010000298 DACDPS010000000
VERSION     DACDPS010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.guided.150"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..615
                     /locus_tag="IWJ07_002968"
     CDS             <1..615
                     /locus_tag="IWJ07_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAP9334456.1"
                     /translation="STDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPAQYDVEL
                     EQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIAEYKAKG
                     YTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYRFGFIRR
                     YPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            633..1460
                     /gene="vanW"
                     /locus_tag="IWJ07_002969"
     CDS             633..1460
                     /gene="vanW"
                     /locus_tag="IWJ07_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HAP9334457.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATIPEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            1457..2428
                     /locus_tag="IWJ07_002970"
     CDS             1457..2428
                     /locus_tag="IWJ07_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HAP9334458.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTARGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            2421..3449
                     /gene="vanB"
                     /locus_tag="IWJ07_002971"
     CDS             2421..3449
                     /gene="vanB"
                     /locus_tag="IWJ07_002971"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HAP9334459.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKFG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQIIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTMPGFTSYSRYPRMVAAAGITLPALIDS
                     LITLALKR"
BASE COUNT          986 a          727 c          953 g          814 t
ORIGIN      
        1 agcactgacg tagtggagaa tttggaggaa aacgctcttg ccaccgccaa agaacaggga
       61 gatgaacagg aatggagcct gattttagtg aacaggcaga accccatccc cgcacagtac
      121 gatgtggagc ttgagcaact atcaaatggt gagcggatag atattcggat ttctccctat
      181 cttcaagatt tgtttgatgc cgcaagaact gatggagttt acccgattgt cgcatccgga
      241 taccgaacaa cagaaaaaca gcaagaaatt atggatgaaa aaattgccga atataaggcg
      301 aaaggctaca cctctgcaca ggctaaagcg gaagcagaaa cttgggtggc cgtgccggga
      361 acgagcgagc atcagcttgg tcttgctgtg gatatcaatg cggacggaat tcattcaaca
      421 ggcaacgagg tttatagatg gctggatgaa aacagctatc gttttggttt tattcgtcgc
      481 tacccaccag acaagacaga gataaccggt gtgagcaacg agccgtggca ttaccgatat
      541 gttggcatcg aagctgccac agagatgtac aaccaagggg tttgccttga ggaatattta
      601 aaaccagaaa aatgagaaaa ggatgttatg ttatggacag aaaaagattg acacagcgtt
      661 tcccgttcct gcttccaatg agacgggcgc agagaaaaat gtgcttttat gcgggaatga
      721 gatttgacgg ctgtcgctat gcgcagacga taggggaaaa atcgctttcc catttgctct
      781 ttgaaacgga ttgcgcatta tacaaccaca ataccggatt tgacatgata taccaggaaa
      841 acaaggtgtt caacttaaag ctggcagcaa agaccttaaa cggcttattg ataagaccgg
      901 gggaaacctt ttctttctgg tggctggtac gccatgcgga caaagatacc ccctataaag
      961 acggccttac ggtgaccaat ggtaaactca ccaccatgtc gggcggcggt atgtgccaga
     1021 tgagcaattt actattttgg atgttcctgc atacgccatt gacaattatc cagcgcagag
     1081 gtcacgaagt aaaggagttt ccagagccaa acagtgacga gattaaaggg gtggatgcaa
     1141 ccattccaga gggctggatt gatttaaaag tgcgaaacga taccgactgc acctaccaaa
     1201 tatgggtgac cttagatgat gagaaaatca tcggtcaggt gtccgccgac aaagagcccc
     1261 aagcattata caaaattaca aatggcagta tccagtatgt ccgtgaaagt ggcgggattt
     1321 atgaatatgc ccaggttaaa cggatgcaag ttgccttagg taccggggaa ataatagatt
     1381 gcaagctgct ttatacaaac aaatgcaaaa tctgttatcc cctcccggaa agtgtggata
     1441 ttcaggagga gaaccaatga gaaaaagtgt gggcattact gtttttggat gcgaacagga
     1501 tgaggcaaat gctttccgcg ctttatcgcc ggattttcat attatcccta cgctgattag
     1561 cgatgcgata tcggcagaca acgcaaaatt ggccgctggc aatcaatgcg ttagcgtagg
     1621 ccataagtcc gaggtttccg aggcgacaat tcttgcgctg agaaaggtcg gggtaaaata
     1681 catttctacc cgcagcatcg gctgcgatca cattgatacg actgccgccg agagaatggg
     1741 aatctcggtt ggcacggttg cgtattcgcc ggacagcgtt gcggattatg ctttgatgct
     1801 gatgctgatg gccatacggg gtgcaaaacc caccatgcac gccgtggcgc aacaagattt
     1861 cagattggat cgtatccggg ggaaagaact gggggatatg actgtgggag ttattggaac
     1921 cggccatatc gggcaagcgg tcgtcaaaag gctgcgggga tttggatgcc atgtgctggc
     1981 ctatgataac agccgaaaaa tggatgcaga ttatgtccag cttgatgagc ttctaaaaaa
     2041 cagcgatatt gttacgctcc atgtgccgct ttgtgcggat acccgccatc tgatcggtca
     2101 gaagcaaatt ggagagatga agcaaggcgc atttttaatc aacaccgcgc gcggagcgct
     2161 tgtcgatacc ggggcgctgg tggaggcgtt ggaaagcgga aagctgggtg gtgcggcact
     2221 ggatgtgttg gagggcgagg atcagtttgt ttataccgat tgctcgcaga aagtgcttga
     2281 ccatcccttt ttgtcgcagc ttttaaggat gccgaatgtg atcatcacac cccatacggc
     2341 gtactacact gagcgtgtgc tgcaggatac tacagaaaaa acaatcagga attgtctcaa
     2401 ttttgaaagg agtttacagc atgaatagaa taaaagtcgc aatcatcttc ggcggttgct
     2461 cggaggaaca tgatgtgtcg gtaaaatccg caatagaaat tgctgcgaac attgatacgg
     2521 aaaaattcga tccgcactac atcggaatta caaaaaacgg tgtatggaag ctatgcaaga
     2581 agccatgtac ggaatgggaa gccgacagtc tccccgccat actctccccg gataggaaaa
     2641 cgcatgggct gcttgtcatg aaagaaagcg aatacgaaac acggcgtatt gatgtggctt
     2701 tcccggtttt gcatggcaaa ttcggggagg atggtgcgat acaggggctg tttgtattgt
     2761 ctggtatccc ctatgtgggc tgtgatattc aaagctccgc agcttgcatg gacaaatcac
     2821 tggcctacat tcttacaaaa aatgcgggca tcgccgttcc cgaatttcaa attattgata
     2881 aaggtgacaa gccggaggcg ggtgcgctta cctaccctgt ctttgtgaag ccggcacggt
     2941 caggttcgtc ctttggcgta accaaagtaa acggtacgga agaacttaac gctgcgatag
     3001 aagcggcagg acaatatgat ggaaaaatct taattgagca agcgatttcg ggctgtgagg
     3061 tcgggtgtgc ggtcatgggg aacgaggatg atttgattgt cggcgaagtg gatcaaatcc
     3121 ggctgagcca cggtatcttc cgcatccatc aggaaaacga gccggaaaaa ggctcagaaa
     3181 atgcgatgat tacagttccc gcagacattc cggtcgagga acgaaatcgg gtgcaggaaa
     3241 cggcaaagaa agtatatcgg gtgcttggat gcagagggct tgcccgtgtt gatctttttt
     3301 tgcaggagga tggcggcatc gttctaaatg aggtcaatac catgcccggt tttacatcgt
     3361 acagccgcta cccacgtatg gtggccgccg caggaatcac gcttcctgca ctgattgaca
     3421 gcctgattac attggcgtta aagaggtgat aagtatggaa aatggttttt tgtttttaga
//
LOCUS       DACDPS010000299         3332 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.guided.156, whole genome shotgun sequence.
ACCESSION   DACDPS010000299 DACDPS010000000
VERSION     DACDPS010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.guided.156"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..408
                     /locus_tag="IWJ07_002972"
     CDS             <1..408
                     /locus_tag="IWJ07_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP9334460.1"
                     /translation="IPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKIEQADVI
                     ITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIVLSIHLG
                     PIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            666..2168
                     /locus_tag="IWJ07_002973"
     CDS             666..2168
                     /locus_tag="IWJ07_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP9334461.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            2235..2762
                     /locus_tag="IWJ07_002974"
     CDS             2235..2762
                     /locus_tag="IWJ07_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9334462.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            2973..>3332
                     /locus_tag="IWJ07_002975"
     CDS             2973..>3332
                     /locus_tag="IWJ07_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP9334463.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTI"
BASE COUNT         1138 a          568 c          730 g          896 t
ORIGIN      
        1 attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt gggagcgaag
       61 atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa gatcgaacaa
      121 gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca cggaaaagtt
      181 ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc tatttgcgga
      241 agtagagacc aagagctaga aggactttct gattttctcc caattgtctt aagtattcat
      301 ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga aaaaacaaaa
      361 atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt gacaaaatca
      421 ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg tgcattgaaa
      481 aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca aggaagcagt
      541 gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg tgtgtatcca
      601 acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt acgaggtgat
      661 ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc caaggcacat
      721 tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga cttatcggac
      781 gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta ccttatcaag
      841 ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa gataaagaac
      901 gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa atcgaaagag
      961 agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc acactatcgg
     1021 ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact catttcccgt
     1081 taatcgatga accaacgaat catttggata tctctggtag aaaacaagta gcggcttatt
     1141 tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt atcgatgaag
     1201 tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa gggaatttct
     1261 ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa aatgaaaaat
     1321 tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa tggtctcgtt
     1381 cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa tctagacgag
     1441 tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc aaagcaatcg
     1501 tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat atcgaatata
     1561 tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta agcgtagaag
     1621 atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca atcgagcctc
     1681 atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt atccattatc
     1741 ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa catctgagca
     1801 ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa tttgcagaga
     1861 aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg atggaaagag
     1921 atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa gtggaattgg
     1981 ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg aattatttgg
     2041 atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct gccatgttac
     2101 tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt tctcttgaga
     2161 gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac tttgccaacg
     2221 aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg aacgcacaag
     2281 gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg caggcacaga
     2341 aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc cattcccctc
     2401 ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg ccgctgaccg
     2461 ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca agcaaagatg
     2521 agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg gagtcagtct
     2581 tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac gaagaaaatg
     2641 tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat tttcatccga
     2701 tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt tcttatgagt
     2761 aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg cttagcagtg
     2821 ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag aaatgaaatt
     2881 taattatttt aataaaagta ttgaaatttt gtttctttag atatatactc tagtcataga
     2941 aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata tttatgaacg
     3001 attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat ggctttgtcg
     3061 gtattttcac ttccatgcat ataccagaag atgatgtttc ggcatatcaa aagcgattga
     3121 tcgaactagg aagcgaagcg aaaaagaata aactagaatt gatggtcgat atttcaggag
     3181 aggcattgga acgagcgggt ttttcattag aagatctaac gcctttgaaa gcaattggtg
     3241 taacaggttt aagaatggat tatcatattt ccaatcagca gattgccgac tggtcacatc
     3301 aattgaaaat cagtttgaat gctagcacga tc
//
LOCUS       DACDPS010000300         3096 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.guided.162, whole genome shotgun sequence.
ACCESSION   DACDPS010000300 DACDPS010000000
VERSION     DACDPS010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.guided.162"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            161..292
                     /locus_tag="IWJ07_002976"
                     /pseudo
     CDS             161..292
                     /locus_tag="IWJ07_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            307..1176
                     /locus_tag="IWJ07_002977"
     CDS             307..1176
                     /locus_tag="IWJ07_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAP9334464.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARSTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1157..1891
                     /locus_tag="IWJ07_002978"
     CDS             1157..1891
                     /locus_tag="IWJ07_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9334465.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            1924..2832
                     /locus_tag="IWJ07_002979"
     CDS             1924..2832
                     /locus_tag="IWJ07_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAP9334466.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            2829..3077
                     /locus_tag="IWJ07_002980"
     CDS             2829..3077
                     /locus_tag="IWJ07_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin acetyltransferase"
                     /protein_id="HAP9334467.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLLTRGAS"
BASE COUNT         1084 a          445 c          673 g          894 t
ORIGIN      
        1 atcaggcatt tcctttctac agagtacgtc cgataagcta ataagcccat aggcaccacg
       61 ctccttcaat tggatttgcc aaaccgccac aaactttcta cagtaacatt atatcataga
      121 ataaagaact ttagattgac taaaaaagct aattagattt tttaataaaa aattacaacg
      181 agaaacagta accttggatg agttgaaggc cattgctgat gtgctaggtg tcaagtttgt
      241 gcaggcattt attttaccag atggagatga aataaaaata tctaacgaat aaaggaggat
      301 gtttatatgg taggtaatgt tattcaaata gtaacagaga aattatcatc tttgcctttt
      361 atagaaggca ttgtattagg gggctcacgt gcaagaagca cccatacaga aaattctgat
      421 atagatatcg gaatctatta caattcagac tcatttgacc tgacagcgat taatcaaatt
      481 gctacagagt tggatgatga gaatagaaac aaccttgttg tacctcccgg agcgtggggt
      541 gattgggtta atggcggtgg atggttagtt ataaacgggt gtcatgttga cttgatatta
      601 cgtgatataa aacgggtgga acaaataatc aaagatacgg agcaaggaat tgttactgcc
      661 aattatcaga ccgggcatcc ccatggttat ataagtgcta tgtatcgtgg agaattagcg
      721 attagtaaaa tactatatgc taagaacgaa agcttatgcg aattaaaaaa gcaggcagaa
      781 atttacccta ctgctctaaa gaaaagcttg atgaactttt ttatatttga agctgaattc
      841 tctttaatgt ttgtaaaagc aaatgcgggg gcagaggata aatattatat tgcaggccat
      901 gtttttcgta ttatttcatg cttaaatcaa gtactatttg catgtaataa tgcttattgc
      961 attaacgaaa agaaagctat aaaactgctt gaaacttttg aatataaacc tgaaaaatat
     1021 gccgaaagag ttaatcatat ttttgaagta ctcggtcttt ctctttttga atgctacgac
     1081 atgaccgaga aactttataa agaagtgaat gaaattgtat cggagataaa taacttttta
     1141 aacgaggaga gttcagatga aagaaaacaa atatgatgat aatatatttt ttcaaaaata
     1201 cagtcaaatg agtcgctcgc agaaaggact ggctggtgcg ggagaatggg agactttgaa
     1261 aaagatgcta cctgatttta agggtaagcg tgtgcttgat ttaggatgcg gctatggatg
     1321 gcactgtata tatgcgatgg aaaacggtgc ttcctctgta gtaggtgttg atatttctca
     1381 taaaatgctc gaagtagcaa aaggaaaaac ccattttcca cagattgaat atgaatgctg
     1441 tgccatagaa gatgtggatt tcccagagga gagctttgat gtaatactaa gttcgcttgc
     1501 gtttcattat gtagcagact atgagaattt aataaaaaag atatatagga tgctgaaggc
     1561 tggtggcaat ttagttttta cagttgaaca tcctgttttt actgctcatg gaacacaaga
     1621 ctggtattat aacgaaaaag gagaaatact gcatttcccg gtggacaatt attattatga
     1681 gggcaaacgg acagctatgt ttttggaaga aaaggttaca aaatatcata gaacactgac
     1741 cacatatcta aatacactgc tttcaaatag ttttataata aatcagattg tggagccaca
     1801 gccgccagag aacatgatgg atattccggg gatggcggat gaaatgcgac gcccaatgat
     1861 gctgattgta tcggcaaaaa agaagatgta ataatataga aaaaataaac gaggagtatg
     1921 taaatgagat cagaaaaaga aatgatggat ttagtacttt ctttagcaga acaggatgaa
     1981 cgtattcgaa ttgtgaccct tgaggggtca cgcgcaaata ttaatatacc taaagatgaa
     2041 tttcaggatt atgatattac atattttgta agtgatatag aaccgtttat atctaatgat
     2101 gactggctta atcaatttgg gaatataata atgatgcaaa agccggagga tatggaatta
     2161 ttcccacctg aagaaaaggg attttcctat cttatgctat ttgatgatta caataaaatt
     2221 gatcttacct tattgccctt ggaagagtta gataattacc taaagggcga taaattaata
     2281 aaggttctaa ttgataaaga ttgtagaatt aaaagggaca tagttccgac tgatatagat
     2341 tatcatgtaa gaaagccaag cgcaagggag tatgatgatt gctgcaatga attttggaat
     2401 gtaacacctt atgttattaa aggattgtgc cgtaaggaaa ttttatttgc tattgatcat
     2461 tttaatcaga ttgttcgcca tgagctgctg agaatgatat catggaaggt cggcatcgaa
     2521 acaggcttta aattaagtgt aggcaagaac tataagttta ttgaaaggta tatatccgag
     2581 gatttgtggg agaaactttt gtccacctac cggatggatt cctatgaaaa catatgggaa
     2641 gcattatttc tatgccatca attgttcagg gcggtatccg gtgaggtggc ggaaaggctt
     2701 cattatgcct atccggagta tgataggaat ataacaaaat ataccaggga catgtataaa
     2761 aaatacactg gtaaaaccgg ctgcctggat agcacatatg ccgctgatat agaagagagg
     2821 cgggaacagt gattacagaa atgaaagcag ggcacctgaa agatatcgat aaacccagcg
     2881 aaccatttga ggtgataggt aagattatac cgaggtatga aaacgagaat tggaccttta
     2941 cagaattact ctatgaagcg ccatatttaa aaagctacca agacgaagag gatgaagagg
     3001 atgaggaggc agattgcctt gaatatattg acaatactga taagataata tatcttttaa
     3061 cccgtggtgc tagttaattt taatatccta aagaat
//
LOCUS       DACDPS010000301         2726 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.guided.173, whole genome shotgun sequence.
ACCESSION   DACDPS010000301 DACDPS010000000
VERSION     DACDPS010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.guided.173"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..377
                     /locus_tag="IWJ07_002981"
     CDS             <1..377
                     /locus_tag="IWJ07_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP9334468.1"
                     /translation="GDMQCYEYGEEIYNQAYESGKMPKEVRDQQLAAIIPYYKSFSRE
                     EMNDVVKVDSSLAAMQFMLVARDHGYETNPIGGFEADQLAEAFGLDKDRYVPVIILSI
                     GKAVEEGYESVRLAPEKITMFE"
     gene            731..2209
                     /gene="msr"
                     /locus_tag="IWJ07_002982"
     CDS             731..2209
                     /gene="msr"
                     /locus_tag="IWJ07_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP9334469.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            2256..2702
                     /locus_tag="IWJ07_002983"
     CDS             2256..2702
                     /locus_tag="IWJ07_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP9334470.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
BASE COUNT          923 a          435 c          588 g          780 t
ORIGIN      
        1 ttggcgatat gcaatgttac gaatatggcg aagaaatcta caaccaagct tacgaatcag
       61 ggaagatgcc aaaagaagtc agagatcaac aattagctgc gatcattcct tattacaaga
      121 gtttttcaag agaagaaatg aacgatgtag tcaaagtcga ttctagctta gcggctatgc
      181 aattcatgtt agttgcgcgt gatcatggct atgaaacaaa tccaattggt ggattcgaag
      241 cagatcagtt ggcagaggca tttggtttgg ataaagaccg ttatgttcca gttattattt
      301 tatcaatagg aaaagcggta gaagaaggtt acgagtctgt tcgtttagca ccagaaaaga
      361 tcactatgtt cgaataaaag gttttcagtt actattcagt agctattgct ttttttgaat
      421 cgtgtgtggt aacctaataa aaaaggaggt cggaaaaatg actgcatcga tgaaattacg
      481 tttcgaactt ttgaataaca actaaataaa ttcaaaagac atgtataaac aacaatttca
      541 tggatggagt atgtccattt tttcgaacga cagtagaata ggtgaaagct atgacagatt
      601 tggtgattta gtcatagtta tttcgttcag agtaggaaaa gatgtgatgc agtttttttg
      661 caaacagatt tttctgctct tttttagtat cgataattgt tgttttacat gaaataggag
      721 tgtaaaacat atggaaaatt tagcagtaaa tataacaaat ctacaagtca gctttggcaa
      781 ccagctagaa ctatctattg attctcttcg tgtctatcag caagatcgta tagggatcat
      841 aggagaaaat ggtgtcggta aatcgacttt gctcaaacta atagccggtg aactttttcc
      901 cgatcatggg aaaatccaaa cagagatcac cttcaactac ctgcctcaat taacctatct
      961 cgctgaagca aaggacctaa atttggaatt agccagtcat ttccagttaa gactggaaga
     1021 aacttcagag cggaaatgga gcggagggga agaacgaaag atcgagttga tacgtcttct
     1081 ttcttcttat gaacaaggga tgcttctaga cgagccaaca acccatctag atagaaaaag
     1141 tattgatcga ctgattgaag agcttcgtta ttattatggt acgctggttt ttgttagtca
     1201 tgatcgctat tttctagatg agctagcatc gaaaatctgg gaagtaaaag acggagaaat
     1261 ccgagagttt tcggggaatt atagtgccta tctcactcaa aaggaattgg agaaaaagac
     1321 tcagctacga gaagcagagt cgatcatgaa agagaaaaaa cgattggaaa aatcgatcca
     1381 agaaaagaaa aaacaagcgg aaaagttgga aaaagtgtcc agtaaaaaga aaaagcaaca
     1441 aatcagactg gatcggttgt cttcctctaa acaaaaagac agtgtacaaa aagccatcca
     1501 aaagaatgcg aaaacattag agagaagact ccaaaaaata ggagaaacaa ccaagccgca
     1561 gcagatgaaa caaatccgtt ttccagtacc aaaatctctt gaactccaca gccgctatcc
     1621 catcatggga caaaatgtcc aattggaaag aagtgggaga acattactgg taaatggtga
     1681 ttttcagttt tctttaggta aaaaaatcgc gattgtcggt gaaaatggtt caggtaagac
     1741 aaccttatta gaacatatcc gcaaacaagg agaaggaatc cttctctctc cgaaagtaag
     1801 ctttcaagta tatcagcaaa agggttatca aatgacatct gaagaatcca tcattcgttt
     1861 tgtcatgaga caaacagagt tttcagaatc gcttgtccgt agtttgctga atcacttagg
     1921 gtttgctcag gaaactttga cgaaaccgtt atgtacatta agtgggggag aagcgacccg
     1981 tctgacgatt gctttgcttt ttactaagcc aagtaatgtg ttgctgttag atgaaccgac
     2041 taattttatt gatatggcaa cgatcgaagc tttagagaag ctaatgcaaa tatatccggg
     2101 aacgattttg tttacttctc atgattctta ctttgtcgag cgtacggctg atgaagttta
     2161 tgaaataaaa gggcagaaaa taaaaaaagt acttacgaga aatttttaaa tgtgctacga
     2221 tgattttcgt gacaaattta aaggagtggt acagattgcc tacaaataaa aaagaaggaa
     2281 taatttttac aacaatgatg tgttttttga tggtcttggg gatgagcgcg tataatttat
     2341 ggctgcatca agatcttcat ttatcaagtt tgttgattgg tctagtgcct ggattcgtgg
     2401 tggctttcat tttggatgtc ttcatcatcg gtgtgctggc gaaaaaaatt gcctttaaac
     2461 taccagtaaa tccagaaagc aaggtccaaa tgatcttaac tatttcatgc ttgatggttt
     2521 taggaatggt gacatgtatg tctttattcg gtgtgatcat ggaacaagga atacctgatc
     2581 aattattctc tgcttatatg aatgcttgga aaatgaatgt gattgccgct ttaccgttgc
     2641 agttgatcat cgttggcccg ctttcaagaa gtgtgctaaa agcaattcaa agtaatgaat
     2701 gatggaacac tgttatttga aaaaag
//
LOCUS       DACDPS010000302         2520 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.guided.178, whole genome shotgun sequence.
ACCESSION   DACDPS010000302 DACDPS010000000
VERSION     DACDPS010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.guided.178"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            12..77
                     /locus_tag="IWJ07_002984"
     CDS             12..77
                     /locus_tag="IWJ07_002984"
                     /inference="COORDINATES: protein motif:HMM:NF019687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9334471.1"
                     /translation="MSPINYRASIYHWDFYALLGF"
     gene            93..2012
                     /gene="tet(M)"
                     /locus_tag="IWJ07_002985"
     CDS             93..2012
                     /gene="tet(M)"
                     /locus_tag="IWJ07_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAP9334472.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNVCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2131..2298
                     /locus_tag="IWJ07_002986"
     CDS             2131..2298
                     /locus_tag="IWJ07_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9334473.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(2358..>2520)
                     /locus_tag="IWJ07_002987"
     CDS             complement(2358..>2520)
                     /locus_tag="IWJ07_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP9334474.1"
                     /translation="HVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSIMESLASG
                     INEARNIED"
BASE COUNT          862 a          385 c          500 g          773 t
ORIGIN      
        1 aattctgatt catcagtccc ataaattata gagccagtat ttatcactgg gatttttatg
       61 cccttttggg cttttgaatg gaggaaaatc acatgaaaat tattaatatt ggagttttag
      121 ctcatgttga tgcaggaaaa actaccttaa cagaaagctt attatataac agtggagcga
      181 ttacagaatt aggaagcgtg gacaaaggta caacgaggac ggataatacg cttttagaac
      241 gtcagagagg aattacaatt cagacaggaa taacctcttt tcagtgggaa aatacgaagg
      301 tgaacatcat agacacgcca ggacatatgg atttcttagc agaagtatat cgttcattat
      361 cagttttaga tggggcaatt ctactgattt ctgcaaaaga tggcgtacaa gcacaaactc
      421 gtatattatt tcatgcactt aggaaaatgg ggattcccac aatctttttt atcaataaga
      481 ttgaccaaaa tggaattgat ttatcaacgg tttatcagga tattaaagag aaactttctg
      541 ccgaaattgt aatcaaacag aaggtagaac tgtatcctaa tgtgtgtgtg acgaacttta
      601 ccgaatctga acaatgggat acggtaatag agggaaacga tgacctttta gagaaatata
      661 tgtccggtaa atcattagaa gcattggaac tcgaacaaga ggaaagcata agatttcaga
      721 attgttctct gttccctctt tatcatggaa gtgcaaaaag taatataggg attgataacc
      781 ttatagaagt tattactaat aaattttatt catcaacaca tcgaggtccg tctgaacttt
      841 gcggaaatgt tttcaaaatt gaatatacaa aaaaaagaca acgtcttgca tatatacgcc
      901 tttatagtgg agtactacat ttacgagatt cggttagagt atcagaaaaa gaaaaaataa
      961 aagttacaga aatgtatact tcaataaatg gtgaattatg taagattgat agagcttatt
     1021 ctggagaaat tgttattttg caaaatgagt ttttgaagtt aaatagtgtt cttggagata
     1081 caaaactatt gccacagaga aaaaagattg aaaatccgca ccctctacta caaacaactg
     1141 ttgaaccgag taaacctgaa cagagagaaa tgttgcttga tgcccttttg gaaatctcag
     1201 atagtgatcc gcttctacga tattacgtgg attctacgac acatgaaatt atactttctt
     1261 tcttagggaa agtacaaatg gaagtgatta gtgcactgtt gcaagaaaag tatcatgtgg
     1321 agatagaact aaaagagcct acagtcattt atatggagag accgttaaaa aatgcagaat
     1381 ataccattca catcgaagtg ccgccaaatc ctttctgggc ttccattggt ttatctgtat
     1441 caccgcttcc gttgggaagt ggaatgcagt atgagagctc ggtttctctt ggatacttaa
     1501 atcaatcatt tcaaaatgca gttatggaag ggatacgcta tggttgtgaa caaggattgt
     1561 atggttggaa tgtgacggac tgtaaaatct gttttaagta tggcttatac tatagccctg
     1621 ttagtacccc agcagatttt cggatgcttg ctcctattgt attggaacaa gtcttaaaaa
     1681 aagctggaac agaattgtta gagccatatc ttagttttaa aatttatgcg ccacaggaat
     1741 atctttcacg agcatacaac gatgctccta aatattgtgc gaacatcgta gacactcaat
     1801 tgaaaaataa tgaggtcatt cttagtggag aaatccctgc tcggtgtatt caagaatatc
     1861 gtagtgattt aactttcttt acaaatggac gtagtgtttg tttaacagag ttaaaagggt
     1921 accatgttac taccggtgaa cctgtttgcc agccccgtcg tccaaatagt cggatagata
     1981 aagtacgata tatgttcaat aaaataactt agtgtatttt atgttgttat ataaatatgg
     2041 tttcttgtta aataagatga aatatttttt aataaagatt tgaattaaag tgtaaaggag
     2101 gagatagtta ttataaacta caagtggata ttgtgtcctg tatgtggaaa taaaacacga
     2161 ttaaagataa gggaagatac tgaattaaaa aaattccccc tctattgtcc gaaatgcaga
     2221 caagaaaatt taattgaaat aaagcagttc aaagtaactg tgattacaga gccagacgca
     2281 aagacgcaga gccgataaaa tgagattaat acaatctcat tttatcggct ctttccgtta
     2341 tgtatggatt cttttaatta gtcttcgatg tttcttgctt cgttgatacc gctggctaaa
     2401 gattccatta tggatagttc tttgtctgta aagctatcca tgtatttctc tatctgtaat
     2461 cgtcgggtgc tttttaccaa gttattagca ggtaagaaaa attcatcaac ggaaacatga
//
LOCUS       DACDPS010000303         2026 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.guided.192, whole genome shotgun sequence.
ACCESSION   DACDPS010000303 DACDPS010000000
VERSION     DACDPS010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.guided.192"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..201
                     /locus_tag="IWJ07_002988"
     CDS             <1..201
                     /locus_tag="IWJ07_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP9334475.1"
                     /translation="FVHTLGDAHLYINHIEQMKEQLSREIRAFPTLVLNQEKQSVFDF
                     EMEDIQLEGYDPHPAIKAPIAV"
     gene            221..748
                     /locus_tag="IWJ07_002989"
     CDS             221..748
                     /locus_tag="IWJ07_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9334476.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(811..1464)
                     /locus_tag="IWJ07_002990"
     CDS             complement(811..1464)
                     /locus_tag="IWJ07_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP9334477.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            1596..>2026
                     /locus_tag="IWJ07_002991"
     CDS             1596..>2026
                     /locus_tag="IWJ07_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9334478.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKIL"
BASE COUNT          737 a          372 c          401 g          516 t
ORIGIN      
        1 tttgttcaca cattaggaga tgctcatctt tatatcaatc atatcgaaca aatgaaagaa
       61 caattgagcc gagaaatacg cgcatttccg actttagttt tgaatcagga aaagcagtca
      121 gtatttgatt ttgagatgga agatatccaa ttagaaggat atgacccaca tccggcgatc
      181 aaagctccta tcgctgttta gaatcagaaa ggatgagaaa atgttagctg caatttgggc
      241 acaggatgaa aacggattga ttggaaaaga agaccgtctt ccttggcgtt tgccaaatga
      301 tctgaaattc ttcaaacaaa tgacagaagc aaatacacta gtgatgggac gaaaaacatt
      361 cgaagggatg ggtagccgtc cgttgccgaa tcgtcagacg attgttttga cgagagattc
      421 ttcctataaa gcagaaggcg tgcatgtcat gcatagtgtg gaagacgtgc ttgctctgga
      481 aaaagaaaca gacggtatct ttttcatcgc tggagggtca gctgtttatc aagaatttct
      541 gccattttgt acgatattat accgtacagt catccatcat gtattcgatg gagatgctta
      601 ttttccacct gttgactggt ctgattggtc actgatcaat actagtcaag gagaaatgga
      661 tgaaaaaaat cgttatccgc atcaatttga aacgtatcag cgtaatgaga atcctgtaaa
      721 aagcgggaca ggcgaattga cgatataaaa aagctatgga tagactagct gttatttaga
      781 gctgtcccat ccatagcttt atttttttta ttaagtaaag aataaaacag agaagtacat
      841 aaaacctgca cctaacataa caaacaaatg ccaaacaaca tgcatatagc ggatagaatt
      901 caagctataa aagaatgcgc ctatcgtgta agaaagcccg cctgctacca tcaaaataac
      961 accagtcatt ccaagagagt gataaagagg aacagcagcc acgacacaga gccaccccat
     1021 aaaaatatag atgatcgtag atacttttga tactctctcc cgtttatgca acgtcaacgc
     1081 tttatatata atgccaagaa tcgcaaacaa ccagattaat acaaataaag tccaacctag
     1141 ccatcctcgg atactcagca aacaaaaagg agtataactt cctgcaatca aaagaaaaat
     1201 cgaactatga tcgaatactt ggaacacctt ttttgctttt gtaaaaatca atgaatgaaa
     1261 caaagtagac gttaaaaata aaagaatcat catcgatcca tagatagcat aagagacaat
     1321 atggagggag gaatgcaagt gggcaccttt tactaaaaga accactagtc ccgcgatact
     1381 caatccagtc ccaattccat gggtgactgc attaaacact tcatttaata tttcatacgt
     1441 tcgattaaat ttcgtttttt ccatgaaaac ggctccttta ctaggtgtaa ttaatgaaaa
     1501 atcattattt gtctgttata ctaaaaatag aaattgtctt acatgtagtt taacatgaat
     1561 aaggcatctt gttaaagaaa gagagagaga aaataatgac aaatgtaaaa atcgtaacag
     1621 attcttcatg tacgatggaa aaaaatgtac gcgatgcttt aggcattcgt gtcatgccgc
     1681 tatccatcat gatcgacgga acagtatatc ccgatgatga ccaactagac ggcgaaagat
     1741 ttatggaaat gatggcgaaa gcaaaaaatc tgcccaaaac aagccaacca ccaattggag
     1801 aattcatctc gatgtatgat gagctaggag aagacggcag tgaagtgatt tcgatccata
     1861 tgacaaaagg cttgagtgga acagtagaag cagcaagaca agccagtaat ctaacaaaaa
     1921 cgaaagtaac ggttatcgac agtgatacca ctgaccaagg attatctttt caggtaattc
     1981 gagcagctga attagcaaaa gaaggtaaaa cagtagagaa aatcct
//
LOCUS       DACDPS010000304         1504 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.guided.227, whole genome shotgun sequence.
ACCESSION   DACDPS010000304 DACDPS010000000
VERSION     DACDPS010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.guided.227"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            262..373
                     /locus_tag="IWJ07_002992"
                     /pseudo
     CDS             262..373
                     /locus_tag="IWJ07_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787626.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            394..477
                     /locus_tag="IWJ07_002993"
     CDS             394..477
                     /locus_tag="IWJ07_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAP9334479.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            602..1339
                     /gene="erm(B)"
                     /locus_tag="IWJ07_002994"
     CDS             602..1339
                     /gene="erm(B)"
                     /locus_tag="IWJ07_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP9334480.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          551 a          224 c          245 g          484 t
ORIGIN      
        1 atttgtatcg ataagaaata gatttaaaaa tttcgctgtt attttgtaca tttaacttga
       61 cggtgacatc tctctattgt gagttattag tggtacagtt ttcaaccgtt ttaattataa
      121 aaaagtggtg catttttaaa ttggcacaaa caggtaacgg ttattgcagg tgtatttctt
      181 atctatgggt ttaacatgga ttttatcatt aaaatcatga gtattgtccg agagtgattg
      241 gtcttgcgta tggttaaccc taaagttatg gaaataagac ttagaagcaa acttaagagt
      301 gtgttgatag tgcattatct taaaattttg tataatagga attgaagtta aattagatgc
      361 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgttatgta
      421 gataaaacat ctactgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      481 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      541 acaaacaaat cgtttaactt ctgtatttgt ttatagatgt aatcacttca ggagtgataa
      601 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      661 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      721 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      781 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat
      841 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      901 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccgtgcgtc
      961 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
     1021 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
     1081 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
     1141 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
     1201 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1261 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1321 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
     1381 ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta ccaattaatt
     1441 cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc ttatcgatac
     1501 aaat
//
LOCUS       DACDPS010000305         1286 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.guided.241, whole genome shotgun sequence.
ACCESSION   DACDPS010000305 DACDPS010000000
VERSION     DACDPS010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.guided.241"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            47..439
                     /locus_tag="IWJ07_002995"
     CDS             47..439
                     /locus_tag="IWJ07_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9334481.1"
                     /translation="MEEVLIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            440..>1286
                     /gene="aac(6')-Ie"
                     /locus_tag="IWJ07_002996"
     CDS             440..>1286
                     /gene="aac(6')-Ie"
                     /locus_tag="IWJ07_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ie"
                     /protein_id="HAP9334482.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFL"
BASE COUNT          525 a          102 c          220 g          439 t
ORIGIN      
        1 aacattgtat agcattttac acaggagtct ggacttgact gagtttatgg aagaagtttt
       61 aattgatgat aatatggttt ttgatattga taatttaaaa ggatttctta atgataccag
      121 ttcatttggg tttatagcta aagaaaataa taaaattata ggatttgcat attgctatac
      181 acttttaaga cctgatggaa aaacaatgtt ttatttacac tcaataggaa tgttacctaa
      241 ctatcaagac aaaggttatg gttcaaaatt attatctttt attaaggaat attctaaaga
      301 gattggttgt tctgaaatgt ttttaataac tgataaaggt aatcctagag cttgccatgt
      361 atatgaaaaa ttaggtggta aaaatgatta taaagatgaa atagtatatg tatatgatta
      421 tgaaaaaggt gataaataaa tgaatatagt tgaaaatgaa atatgtataa gaactttaat
      481 agatgatgat tttcctttga tgttaaaatg gttaactgat gaaagagtat tagaatttta
      541 tggtggtaga gataaaaaat atacattaga atcattaaaa aaacattata cagagccttg
      601 ggaagatgaa gtttttagag taattattga atataacaat gttcctattg gatatggaca
      661 aatatataaa atgtatgatg agttatatac tgattatcat tatccaaaaa ctgatgagat
      721 agtctatggt atggatcaat ttataggaga gccaaattat tggagtaaag gaattggtac
      781 aagatatatt aaattgattt ttgaattttt gaaaaaagaa agaaatgcta atgcagttat
      841 tttagaccct cataaaaata atccaagagc aataagggca taccaaaaat ctggttttag
      901 aattattgaa gatttgccag aacatgaatt acacgagggc aaaaaagaag attgttattt
      961 aatggaatat agatatgatg ataatgccac aaatgttaag gcaatgaaat atttaattga
     1021 gcattacttt gataatttca aagtagatag tattgaaata atcggtagtg gttatgatag
     1081 tgtggcatat ttagttaata atgaatacat ttttaaaaca aaatttagta ctaataagaa
     1141 aaaaggttat gcaaaagaaa aagcaatata taatttttta aatacaaatt tagaaactaa
     1201 tgtaaaaatt cctaatattg aatattcgta tattagtgat gaattatcta tactaggtta
     1261 taaagaaatt aaaggaactt ttttaa
//
LOCUS       DACDPS010000306         1219 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.guided.244, whole genome shotgun sequence.
ACCESSION   DACDPS010000306 DACDPS010000000
VERSION     DACDPS010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.guided.244"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            46..297
                     /locus_tag="IWJ07_002997"
                     /pseudo
     CDS             46..297
                     /locus_tag="IWJ07_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            390..1184
                     /locus_tag="IWJ07_002998"
                     /note="aph(3')-IIIa"
     CDS             390..1184
                     /locus_tag="IWJ07_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP9334483.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          388 a          208 c          298 g          325 t
ORIGIN      
        1 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
       61 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      121 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      181 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      241 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      301 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      361 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      421 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      481 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      541 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      601 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      661 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      721 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      781 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      841 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      901 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
      961 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1021 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1081 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1141 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1201 tctaaaaagc tttaactac
//
LOCUS       DACDPS010000307          942 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.guided.257, whole genome shotgun sequence.
ACCESSION   DACDPS010000307 DACDPS010000000
VERSION     DACDPS010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.guided.257"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            15..623
                     /locus_tag="IWJ07_002999"
     CDS             15..623
                     /locus_tag="IWJ07_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HAP9334484.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
BASE COUNT          256 a          189 c          240 g          257 t
ORIGIN      
        1 aagaggtgat aagtatggaa aatggttttt tgtttttaga tgaaatgttg catggcgtcc
       61 gttgggatgc caagtacgcc acatgggata acttcacagg aaaaccggtg gatgggtatg
      121 aggtgaatcg catcatcggc acaaaggccg tggcgtttgc tctgcgcgag gcacaaatcc
      181 atgcggctgc gcttggctat ggcttgcttt tatgggatgg atatcggcca agaactgcgg
      241 tggactgctt cctgcgttgg gcagcgcaac cggaggacaa tctcacaaaa gaaaaatttt
      301 accccaatat agagcgagcc gagttgatta caaagggtta tgtggcctca caatccagcc
      361 atagccgtgg aagcgcaatt gatcttacgc tctaccacct ggatacaggg gaacttgttt
      421 caatgggaag taacttcgat tttatggacg aacggtcgca tcatacagca aaagggatag
      481 gggatgcaga ggcacaaaat cgaagatgct tgcgtaaaat catggaaagc agcggatttc
      541 agtcttatcg ctttgaatgg tggcactata agttgattga tgagccatac cccgatacct
      601 attttaattt tgccgtttca taatgaaagt attggttttg tatcagattt tggatttgat
      661 aaaatctatg ctggtcatgg acaatctttt actggaaagt ggttaaagca tcttttgtaa
      721 tacattgcaa attatagtat tcaatctgcc cagcggcaga aaagaaaaaa accgctgttg
      781 ggcagacctt tcccacgccc attttgtatt ttgctggcgg cggttttgag aaatcaaggc
      841 cgccgttctt ttctggcagc aaaaggtgtg gcggcccata ggaggatgcc gctatgctgt
      901 tttgctattg ccatgctcta atccacttcc accactcaaa aa
//
LOCUS       DACDPS010000308          773 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.guided.268, whole genome shotgun sequence.
ACCESSION   DACDPS010000308 DACDPS010000000
VERSION     DACDPS010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.guided.268"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            184..732
                     /gene="aac(6')"
                     /locus_tag="IWJ07_003000"
     CDS             184..732
                     /gene="aac(6')"
                     /locus_tag="IWJ07_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP9334485.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          273 a          115 c          170 g          215 t
ORIGIN      
        1 attgatggct ttcctgatct ttcctatttt gaataaagtg tttctgtata atttttttat
       61 tttataagta agataaataa tgaaagaata gagatactga ttggtagcta aagatttttg
      121 agcgagaaca tttcgttttt tcaaaaggac tgttagaata agcaagaaca agggggaaga
      181 cgtatgataa tcagtgaatt tgaccgtaat aatccagtat tgaaagatca gctttctgat
      241 ttactgagac tgacttggcc ggaagaatat ggagacagct cggcagaaga agtagaagaa
      301 atgatgaatc cagaacgaat cgcggtagca gcggtagacc aagatgagtt agtaggattt
      361 attggtgcaa tccctcaata cggtatcaca ggttgggaat tgcatccatt agttgtagaa
      421 agctcccgac gaaagaacca aataggtact cgattagtca attacttaga aaaagaagta
      481 gcttccagag gaggaatcac gatttattta ggtacggatg atttagacca tggaacaacg
      541 ttaagtcaaa cggacctgta tgagcataca tttgataaag tggcttccat ccagaacctt
      601 cgtgaacatc cgtatgaatt ctatgaaaaa ttaggttata aaatcgtagg tgtcttacca
      661 aatgcaaatg gctgggacaa accggatatt tggatggcaa aaacgattat tcctcgacca
      721 aattctcaat aataaaaaga ggttgtgaca gaagtaatca gcttcaagaa ata
//
LOCUS       DACDPS010000309          643 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62508_2015
            SAMN12639594-rid9883683.guided.281, whole genome shotgun sequence.
ACCESSION   DACDPS010000309 DACDPS010000000
VERSION     DACDPS010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639594
            Sequence Read Archive: SRR10030841
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742146.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 06:34:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,001
            CDSs (total)                      :: 2,937
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 29 of 62
            Pseudo Genes (incomplete)         :: 32 of 62
            Pseudo Genes (internal stop)      :: 12 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639594-rid9883683.guided.281"
                     /isolate="E62508_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..466
                     /gene="dfr_DGK"
                     /locus_tag="IWJ07_003001"
     CDS             <1..466
                     /gene="dfr_DGK"
                     /locus_tag="IWJ07_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DfrD/DfrG/DfrK family trimethoprim-resistant
                     dihydrofolate reductase"
                     /protein_id="HAP9334486.1"
                     /translation="NRVIGKENDIPWRIPKDWEYVKNTTKGHPIILGRKNLESIGRAL
                     PDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIYNLFFPYVEKMY
                     ITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYERKNLLS"
BASE COUNT          236 a           82 c          129 g          196 t
ORIGIN      
        1 gaatagagtg attggcaaag agaatgacat tccttggagg attcccaagg actgggaata
       61 tgttaaaaat actacaaagg gacatccgat aatattaggt aggaagaacc ttgaatcaat
      121 cggaagagcc ttacctgaca gaagaaatat tattctgacg agagataagg ggtttacctt
      181 taatggttgt gaaattgttc attcaataga agatgttttt gagttatgta aaaacgaaga
      241 agaaattttt attttcggag gagaacagat ttataatttg tttttccctt atgttgagaa
      301 aatgtacatc acaaaaatac atcatgaatt cgaaggagat actttttttc cagaagtgaa
      361 ttatgaggaa tggaatgagg tatttgccca aaaagggata aagaatgata aaaatccgta
      421 taactactat tttcatgtat atgaaagaaa aaacttattg agttaacggg tgcgttaatt
      481 gaagaagaac agtaaagata aagcacaaga gcttgggatt actccaagct ctttttgtca
      541 tttgaaataa attatatctt tccaatcaaa ttgtttatct gtaatcccta atctttgagc
      601 aggtgtcaaa acctttttat ccgctgattt aaaagggcga caa
//
